LOCUS       DAOQCW010000001        61283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_160_length_61283_cov_5.123065, whole genome shotgun sequence.
ACCESSION   DAOQCW010000001 DAOQCW010000000
VERSION     DAOQCW010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 61283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 61283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61283
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160_length_61283_cov_5.123065"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PLS16_00005"
     CDS             <1..320
                     /locus_tag="PLS16_00005"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HNJ59486.1"
                     /translation="FATHDPTTLNRQGNDVGTQYRSVIFYHNETQKKLATEVISELAL
                     NQIYKQPIVTEIVPISNFYKAEDYHQNYFEENSTSNPYCEIVIQPKVEKFEKLFKNLL
                     KDK"
     gene            complement(405..1223)
                     /locus_tag="PLS16_00010"
     CDS             complement(405..1223)
                     /locus_tag="PLS16_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNJ59487.1"
                     /translation="MLLVDVFPNLLDLSVIISLLTLAFLEIVLGIDNIIFISIVSAKL
                     PKAQQPKARQIGLLLAMFFRIGLLFGITYIIKLTNPLFYIPFIEDNDQPIGISIKDLI
                     LLLGGFFLIAKSISEIHHKLEDSIEQEIGANQKSTVSKAVTSFGTVIVQILLIDAVFS
                     IDSILTAIGLVSNVWIMIFAVIISMVIMMIFANPVAEYIDKNPTLQILALAFLVAIGI
                     ILIAEGFHQHINKGYIYTAMAFGLSVEFLNMRLRKNKKYIQLNDQDTLNDKVVN"
     gene            complement(1301..2350)
                     /gene="rlmN"
                     /locus_tag="PLS16_00015"
     CDS             complement(1301..2350)
                     /gene="rlmN"
                     /locus_tag="PLS16_00015"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNJ59488.1"
                     /translation="MMTNALQNIRQLSLDELQAAMKDLGEPKFRAKQIYEWLWKKSVT
                     DFEAMTNLSKPFREKLAEKFTILPIKLYSAQHSNDGTIKTAFQLHDKHFIEGVLIPST
                     DRATACVSSQVGCSLACKFCATGFLKRERNLEADEIYDQVVFINRQAQESLGRNLTNI
                     VFMGMGEPLLNYSNVMEAINKITSEDGLNMAAKRITVSTAGISKMIKKMADDGVRFNL
                     ALSLHAADDEKRNAIMPINEQNNIASLMEALIYFEQKTKSEITFEYILMKEVNDSIED
                     AKNLVALCKQIPNVKVNLIEYNTVEGVDLQRSSVEKQEAFSKYLSSQRVIANIRRSRG
                     KDIDAACGQLANKMK"
     gene            complement(2385..3182)
                     /locus_tag="PLS16_00020"
     CDS             complement(2385..3182)
                     /locus_tag="PLS16_00020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNJ59489.1"
                     /translation="MNAVTDSLQANYPINKNRTYLFGFSNGGFMVQRMACESNERFAA
                     IASIAGTIGNKIYNCNPGRFLPILHFHGTSDLNVGYYITLFGLNVEDMLSVWTKNNQC
                     SNQPPLRTDIPNIKNDGLTVEHYVYQNCKQPVELFKVNNAEHILLNQENNDIDYAVEM
                     WKFFNPHALNEAEVISANHQFKIKEKIKIYPNPVSNLLTIDVVASEFQNNIDINILDI
                     NGKQVLTFESNKNNIHQIDCSKWSNGAYFIKISDDENFSITKFNVIH"
     gene            complement(3140..3535)
                     /locus_tag="PLS16_00025"
     CDS             complement(3140..3535)
                     /locus_tag="PLS16_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59490.1"
                     /translation="MLKKILLFLCIFVVNFTYAEWLNKSFKHNNLTREYRVYLPDNYD
                     ETKLYKLVVGIHGLGSNMTDFSGAFYNFCKIADTADIILVFPQGYDKFFLGRGWNAGA
                     GTLVNIRVLELMTKVLSMLLQIHFKPTIQ"
     gene            3657..3839
                     /locus_tag="PLS16_00030"
     CDS             3657..3839
                     /locus_tag="PLS16_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59491.1"
                     /translation="MSLLEQKDKPDEKLKSETKKLEYSLDVSEVSEVPTIPTSLMPEL
                     EELVAKNPNRLIGCGG"
     gene            complement(3841..4332)
                     /locus_tag="PLS16_00035"
     CDS             complement(3841..4332)
                     /locus_tag="PLS16_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HNJ59492.1"
                     /translation="MEIISDKRPIPEELKKLQGFVKLMDAQFRIPGTNIRFGIDPLLN
                     LIPYGGAAVGYAIGIYLMFAMYKNGASSKVMGKMIWNVSIDALVGAIPVLGTFFDFYY
                     KANKRNMVLAVEHFEEGKHKGSILPYLIPILIAFLLVLAASIYVTIQVFQFLFQLLGG
                     ITL"
     gene            complement(4339..4809)
                     /locus_tag="PLS16_00040"
     CDS             complement(4339..4809)
                     /locus_tag="PLS16_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59493.1"
                     /translation="MAIPQFNHLAEEEVQLLLDAPAIITLLIGTADGNMDDKEIAAGL
                     SSVIVRKDSNDALLQPYYEAVDKNYDQRLFDFSNQYKDKGTEQRTQELSDVLAGLNDI
                     LIKVDPLFVTVLIKSLKSFAKEIAQASGGMAGIGLMSISNEEEHLISLPMITIK"
     gene            4906..5316
                     /gene="tsaE"
                     /locus_tag="PLS16_00045"
     CDS             4906..5316
                     /gene="tsaE"
                     /locus_tag="PLS16_00045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNJ59494.1"
                     /translation="MAFKVKDLDDLEIVVKELLQYTEKYTIFCFFGEVGAGKTTLITQ
                     FCKALGIDDKVSSPTYSIVQEYKHGNQKIFHIDLYRLNSEEEAVDIGLEDYLYSNNIC
                     LIEWPNDFLNLIPKKFVKIEIENMDGVRNISIEV"
     gene            5816..6811
                     /locus_tag="PLS16_00050"
     CDS             5816..6811
                     /locus_tag="PLS16_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004064250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HNJ59495.1"
                     /translation="MDNQRNKDKMDNKFSDIIQIIKKSRTNAIKAVNSELINLYWNIG
                     EHISLKVEQSEWGKSVVKELADFIQSTEPELKGFSDKNLWRMKQFYEAYKDFPKLSSL
                     LREISWTHNLTIFSRCKTIEEQEFYIKLTKNENYTSRELERQISSSLFERTMIGNSKL
                     STSLREASNDLSNTFKDSYVFDFLNLPETHNETDLQKGLINQMKNFILELGRDFLFIG
                     EEYKVQVGSSDFYIDLLFYHRGLQCLVAFELKADKFKPEHLGQLNFYLEVLDRDVKRQ
                     NENPSIGILLCKDKDSEVVEYALSRNLSPTMVSQYETQLPDKKLLQQKLQQLFED"
     gene            complement(7168..7548)
                     /gene="ssb"
                     /locus_tag="PLS16_00055"
     CDS             complement(7168..7548)
                     /gene="ssb"
                     /locus_tag="PLS16_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNJ59496.1"
                     /translation="MAAIRNQVNLIGNLGNKPEVKTFESGKQIVTFSLATSEYYVDNK
                     GERKQVTYWHNIIAMGKVAKVCENYLDKGSEIALNGKITYRQYEDKNGNKRQITEIVA
                     NEILMLGSKRKEMSEIENEAMSEQ"
     gene            complement(7941..10409)
                     /locus_tag="PLS16_00060"
     CDS             complement(7941..10409)
                     /locus_tag="PLS16_00060"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNJ59497.1"
                     /translation="MKKYFIVSCLLFILQLGFAQNTIHLRGVIRDNQNNPIPNVNIIQ
                     NNTNNVTVSNIDGTYTIRLDFEGNFCEVSFKHLQFDTKTIKIFSSNKMRKEVDVEMLE
                     HIESLDSVEIISKSNSNTNTQIIKGKSVEYFPNPTNDIGTIVKTFAGVQSNNELTGQY
                     SVRGGNYDENLVYINDFEIYKPFLTSSGQQEGLSYANPDLIDQLSFSAGGFESKYGDK
                     MSSVLNVKYKTPEKHGGSIMASLLGVNAHLEGVSKKQKFTYLTGVRYKTNAYVLGSLD
                     RKGEYNPNFFDAQIDLHYKPNNKHDLEILAYHAANHFKFTPELQETKTGTFNQLIRFL
                     VDFDGGENSRFKNSMAGLSYKFVPNAKWSLKFNSAFWKMNEVESFNIIGYYALDEIEI
                     DPNKDNFGNVKNSLGAGTFQNWARNSLEATVFNASLSAKVALNKNLLEFGNTVQYEHI
                     DDLMSEWTRVDSAGFSIPYYGNSVATQDRIKSAIQLSSVRTHGYFQNTWNIRQDSVKL
                     SLTTGIRYHFWNVNKEILVSPRIQVFYQPDLKADVSFRFAAGLYQQAPFYREMRSFDG
                     RINRQVKAQKTAQIIVGSEYNFNAWGNRNFKLTTEAYYKYIWNLNPYEIQDIRIRYFA
                     TNAAKGYATGVDVRLFGELVKGTDSWISFSYLRTRENLDNDFYYRVNDINGVPDSQMV
                     YPGMIRRPTDQAFFMNLFFQDYLVKNKSFKVHFNLVVGTGLPFGPPDRQRYKDVLKMP
                     PYRRLDIGFSYELLNGEKRGLKKPNSFGSHFKNIWTSFEVFNIIGIYNTLSYRWIKDI
                     DNNQWAIPNYLTNRRFNLKLNITW"
     gene            complement(10508..12364)
                     /gene="htpG"
                     /locus_tag="PLS16_00065"
     CDS             complement(10508..12364)
                     /gene="htpG"
                     /locus_tag="PLS16_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNJ59498.1"
                     /translation="METGKINVQTENIFPIIKKFLYSDHEIFLRELVSNAVDATKKLK
                     GLSQLGEVKGEIGDTTIQVKLDKENKTLTISDKGIGMTADEVKKYINQIAFSSAGEFV
                     EKLKNTDSKNIIGHFGLGFYSAFMVAEKVEIQTKSYKDEAAAKWICDGSTDYTLETVE
                     KQERGTDIILHIAEDSLEFLEEQRIKSLLNKYCKFLPIPIQFGYKWQSEEDKKAEKPL
                     EPEIINNTEPAWVKSPSSLNDEDYKNFYRQLYPMNFDEPLFWIHLNVDYPFNLTGILY
                     FPKLKKNFEVQKDKIQLYSNQVFVTDNVGEIVPEYLTLLHGVIDSPDIPLNVSRSYLQ
                     SDGNVKKINQHISKKVSDKLQEIFKADRKQFEEKWSDIGLFVKYGMISDEKFYERSNS
                     FALFQTTENTFYTYAELKEKVTANQTDKDGNLVILYTSDSEEQHSYIKQAKDYGYEVV
                     QLDTMIDNHFIQQLEMKNEKTHFKRVDADILSNLISKDENVESALSKEQEDALKVLFE
                     NTVEKAKVDIELKALSPNDAPIVITRNEFMRRMQEMQKMGGGSPFMFGGMDEKINLVI
                     NTNHSINHKLLEETGEQQEKHLKQLYDLALLSQNMLKGEALTNFVNRSFELI"
     gene            complement(12486..14021)
                     /locus_tag="PLS16_00070"
     CDS             complement(12486..14021)
                     /locus_tag="PLS16_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNJ59499.1"
                     /translation="MLVKTFGSAVSGVDAITITVEVNVCSGKDYYLVGLPDSAVKESL
                     SRIESALRHNRFTMPRIKIIINLSPANIRKEGSAYDLPIAIGILAASEQIGHSKLEDY
                     IIMGELSLDGKVQAIKGALPIAIQARKEKYKGIILPKQNAREAAIVDGLEVIGVETID
                     DVVDFLEDKKVIEPTVVSTREEFFSNIQHIEFDFADVKGQENIKRALEIAAAGGHNVI
                     LIGPPGAGKTMLAKRLPTILPPLSLSEALETTKIHSVAGRLKANSALLYERPFRSPHH
                     TVSDVALVGGGGHPQPGEISLAHNGVLFLDELPEFKRTVLEVMRQPMEERKVNISRAR
                     FSVDYPANFMLVASMNPCPCGYFNHPEKECVCGPGVVQRYLNKISGPLLDRIDLHVEV
                     TPVNINELSSYKKGEDSKTIRERVIEAREIQSERFKNGEVYCNAQMSTKQTREMCALD
                     ANSQNLLNVAMNKLGLSARAYERILRVARTIADLAKSPNIQTEHIAEAIQFRSLDRDS
                     WAA"
     gene            complement(14315..15145)
                     /locus_tag="PLS16_00075"
     CDS             complement(14315..15145)
                     /locus_tag="PLS16_00075"
                     /inference="COORDINATES: protein motif:HMM:NF025115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HNJ59500.1"
                     /translation="MKKKNLIIVIGLLFIFTACKNNGQNTNAQSKDAIYTDTLAYEMK
                     SIKRDTVVKGASEYDTTSIEFIYPLFDIHSAFGKQLNDSIIKRVSLIGEENESGYSTI
                     QSIVDDFMQQYKSYVEESPMDFPWYNQTEINILNNNSNIISFRMALESYTGGAHGSHY
                     VIFDNYNRKTNTLIKIEDVLKDKHDKALLEIGEFYFKQIREIPQKESLQENGAFEAGW
                     LDENQKAGFYFNDNFTIDKDNLTFYYNEYEVSSYAFGPTELKIPLEKIRPYLKNSFGF
                     "
     gene            complement(15162..15884)
                     /locus_tag="PLS16_00080"
     CDS             complement(15162..15884)
                     /locus_tag="PLS16_00080"
                     /inference="COORDINATES: protein motif:HMM:NF025115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HNJ59501.1"
                     /translation="MKIVDRIYPMQFHKDKDTTIIKIAYPFFDSSSALGKNLNDTVRE
                     RIILGKEDEEGNIIHINYHTIEDFVDSLLHEYKLFLGDSPISIPWYNYSNVEVLNNTP
                     SLLSLKSFIRIYLGGMHEDISVVFDNFDTKSGKRLTIDDIFKNKRDENLLKIGEFYFK
                     ETRKIAQDKKLTDLGIFNAGWSATNPKGGFYFNDNFLIDKNYLIFYYNEYEISSYSFG
                     TTELKIPLAEIRPYLKDSFKYE"
     gene            complement(16077..16754)
                     /gene="cmk"
                     /locus_tag="PLS16_00085"
     CDS             complement(16077..16754)
                     /gene="cmk"
                     /locus_tag="PLS16_00085"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNJ59502.1"
                     /translation="MEQINIAIDGYSACGKSSTAKIIAQRLNYVYVDTGAMYRAVTFY
                     FINNKIDLKDEQAVKNALSNINIEFKREREQLLIYLNGVDVSQAIRQMEINQLVSEVS
                     TITAVRRKMVQQQQLMAQKKGVVMDGRDIASVVMPDAELKFFMIADIDIRTKRRMKEL
                     NLEDRPEIFEITKANLLHRDHIDSTRADSPLVQVEGAIVIDTSHITMEEQTNKILSMV
                     DKLNITK"
     gene            complement(16758..17795)
                     /gene="porQ"
                     /locus_tag="PLS16_00090"
     CDS             complement(16758..17795)
                     /gene="porQ"
                     /locus_tag="PLS16_00090"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HNJ59503.1"
                     /translation="MIKQYIFLLCLFSVSTIFGQIGGKSVFQFLKFPISARNEALGGG
                     VLSTIDNDLTSAISNPSLIDSALNKQIAFSNSFFIGDINIGNLTYAQYHKRIKSTLTY
                     TAQYASYGKFESRDNAGNLLGEFRAADFNFQVGTAHYWQHLYYGMNLKLIYSNLAQYN
                     SLALATDIALGYHNKDKKISFSVLLRNAGFQLKRYDKNSEREKLPLQVDVSFYKKFTK
                     IPIALIIVAHDLQKFKLTVSGSATTNAFGKTVVNKRKEFANDILGHFIIATEIDAGKP
                     VRLRVGYNHLRRIELASTQKKGMAGITAGLGLNIRQFSIDYGFGAYHAAGSDHFLSLR
                     IKIDEFGKKAK"
     gene            18021..18290
                     /gene="rpsO"
                     /locus_tag="PLS16_00095"
     CDS             18021..18290
                     /gene="rpsO"
                     /locus_tag="PLS16_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNJ59504.1"
                     /translation="MSLTSEKKIEIFKEFGGSEKNTGSVESQVALITHRINHISAHLR
                     ENRKDHSSTRSLYKLVGQRKKLLKYLANQDITKYRALIEKLNLRK"
     gene            18359..20491
                     /locus_tag="PLS16_00100"
     CDS             18359..20491
                     /locus_tag="PLS16_00100"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNJ59505.1"
                     /translation="MIQFNIQKTKTIYNNGQEVTIETGKLARQADGAVVLTVGKCMIL
                     ATVCASDAPKEGVDFFPLTVDYQEKFSAAGRIPGSFHRREAKLSDYEVLISRLVDRAI
                     RPMFADNFVNDTQVLISLISSDETIAPDTLVGLAASAALSVSSIPFEGPISEVRVIKS
                     KGEFIVNPLVAQMEDAELNIILAGTLDNILMVEGEAAEASEADLIEAIKTGHAVIKQH
                     CEAQLELQKAVNKAKREVPEPESDEDLYQSVKTFAEDKIKEIAKSSTGKAERKAAFKA
                     IKEAYLETISEEAAETFNKGLFSKYYAKVEKNAIRHTVLDDKIRLDGRKTDEIRPLSI
                     EVDVLPTPHGSALFTRGETQSLSTVTLGSKQDEQMLDKAESLTYAKFLLHYNFPPFST
                     GEVKPMRGTSRREVGHGNLALRSLKQMMPADDTNPYTVRIVSDILESNGSSSMATVCA
                     GSLALMDAGIPFKKLVSGIAMGMIAEDGKYAILSDILGDEDHLGDMDFKVTGTKDGIC
                     GVQMDLKVDGLSYEVLAEALEQARKGRLHILNAMEQVIAEPRPELKPHVPRAHVMEVP
                     KEFIGAIIGPGGKIIQEIQEKTNTTITIEEFKEKGIGQVIILSNNKDNMNAAINRIKG
                     IVAVPEVGEVYEGKVKSILAFGAFVEFMPGKDGLLHISEVSHKRLENLDGIIKEGDIV
                     QVKLIGIDPKTGKFKLSRKVLINKNEEA"
     gene            complement(20791..21270)
                     /gene="coaD"
                     /locus_tag="PLS16_00105"
     CDS             complement(20791..21270)
                     /gene="coaD"
                     /locus_tag="PLS16_00105"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNJ59506.1"
                     /translation="MKKIAIFPGSFDPITIGHVDVVKRAAKVFDEIVVAIGVNTQKKY
                     LFDLEERTNWIKKVFANETNVRVETYQGLTVNFCKEIGANYIIRGIRSSADFEYEKTI
                     AQLNQMMHAEVETFLILSSPELSAISSTIVREIIIGKGDVSKFIPHEICEAVIKSLH"
     gene            complement(21272..21811)
                     /locus_tag="PLS16_00110"
     CDS             complement(21272..21811)
                     /locus_tag="PLS16_00110"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HNJ59507.1"
                     /translation="MRINAGKLKGKHFSPPKNIPARPTTDIAKEALFNILTNHIDYES
                     INFLDLFAGTGNIGIEMFSRGCNQITSVDLSPISIKFIQEMSAMAQVPNHQIIKADAI
                     RYIKQTPYKYDLIFAGPPYPLECIDEIPDLIFEYKLLTENGWFIMETSPRHSFLEHPN
                     FFNLRKYGQTHFWFFKYAD"
     gene            complement(21801..22586)
                     /locus_tag="PLS16_00115"
     CDS             complement(21801..22586)
                     /locus_tag="PLS16_00115"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HNJ59508.1"
                     /translation="MWIHLQPYAITYLLFDTTNSKVLDIDSVIINKNFENIRYDEVLP
                     KIFSNENEYFKLSFVKVRITFSTNQFMLLPEQTENPNQMMSFLGMHTLPMDKILSDKI
                     SEEQYLYYVLPIDVYNFFIHRFVNVVWHFGDLALLKYTNSKLSFQSHLLAHIAGNDLT
                     IVLKDKNKVSYFNKFYVQDKQDLLYFLKLAYETLGLDTNQFATYLYGFIEEKSPMYTF
                     SYSYIRNFEIDRILHHDLGYSFYYTNDPLHYYYNLLCLGKYEN"
     gene            22768..23961
                     /gene="sucC"
                     /locus_tag="PLS16_00120"
     CDS             22768..23961
                     /gene="sucC"
                     /locus_tag="PLS16_00120"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNJ59509.1"
                     /translation="MNLHEYQGKELLKSFGVKIQEGIVAETLEQANQAYQTIATRTGK
                     DGVVIKAQVHAGGRGKGGGVKFTPNFDSFKEKAAQILGMTLVTPQTGAQGKVVHKILI
                     AEDMYYKGETEPKEFYLSVLLDRNKECNAIIYSQDGGMDIEEVAEKTPERIFKEWINP
                     MVGIQPFQARRIAFNLGLEGQAFKEMVNFAMNVYKAYEKSDASLFEINPVLKTSDNKI
                     VAVDCKVNLDDNALYRQKDLEAQRDLLEEDPTEVEAQEADLNYVKLDGNVGCMVNGAG
                     LAMATMDIIKLSGGEPANFLDVGGTANAERVEKAFRIIMKDENVKAILINIFGGIVRC
                     DRVAQGVIDAYKNIENINIPIIVRLQGTNADIAKKMIDESGLKVQSAILLSEAAELVN
                     KAVAN"
     gene            24173..25471
                     /locus_tag="PLS16_00125"
     CDS             24173..25471
                     /locus_tag="PLS16_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HNJ59510.1"
                     /translation="MSTQKFETLQVHAGQEPDPTTGSRAVPIYQTTSFNFKSTEHAAN
                     LFGLKEFGNIYTRIMNPTTDVLEKRIAALEGGVAGLATSAGMSAQFLALTNILEAGEN
                     FVTTSFLYGGTYSQFNVSFRRLGIDARFADGDNVDSFEKLIDDKTKAIYLESYDNPRG
                     NVPDWDKFAALAKKYDIPLVVDNTFGICGYMFRPFDHGANVVVAATTKWIGGHGNSIG
                     GIIVDGGNYNWGNGKFPRFSEPSDSYNGLNFWNVFGTPGPFGNIAFAIKARVEGLRDY
                     GNAISPFNSFLLLQGLETLSLRAERHNENTIALANWLNEHPKIEKVNFTGLPDSPYYE
                     LAKKYLRKGHFGYVFNFEIKGGYEAAKTFVDNLKMISNLANVGDAKTLVIHAASTTHE
                     QLSPEQQLSAGVLPASVRISVGIEHIDDIKADIEQALALV"
     gene            25503..26519
                     /gene="metX"
                     /locus_tag="PLS16_00130"
     CDS             25503..26519
                     /gene="metX"
                     /locus_tag="PLS16_00130"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HNJ59511.1"
                     /translation="MEPNILKYKAPFPLESGTILPEIDIAYNTFGTLNADKSNVVWIC
                     HAFSANSNPTEWWPGMVGDGLFFDPKDYFIVCANMLGSCYGSTGPLSINPETGKKYYK
                     DFPVVTVRDMVKSLTILRKHLGIEKIYFSCGFSMGGQQLLEWIITEPTIFKNILLGAT
                     NIKHSPWGIAFNESQRMAIEADGTFGQEDDNAGINGMKAARSIGLISYRNYKAYNITQ
                     HDEDNSKLENFKACGYQRYQGEKLAKRFNAYSYYILSKAMDNHNIERGRGAAKEVLEN
                     IKSNIIVLGIKSDFLYPLEEQELMASLMPHSTFVAIDSHFGHDGFLIENEQISKILST
                     FLKA"
     gene            26603..27637
                     /locus_tag="PLS16_00135"
     CDS             26603..27637
                     /locus_tag="PLS16_00135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNJ59512.1"
                     /translation="MLKIAVVILNYNGKHWLEKFLPNVIENTTYSNSEIIVADNASTD
                     DSIIFLKTNFPTVKIIQNKENTGYAGGYNEALKSVNADVYCLLNSDVEVSKNWLEYIL
                     PTFEDEKVMATQPKILSYHNKSAFEYAGAAGGFIDKYGYPFCRGRIFDKVEQDTGQYN
                     EVLEIFWASGACLFVRSSIFHQLGGFDEDFFAHQEEIDLCWRIKNLGYKILYAPKSVV
                     YHVGGGTLNKSNPKKTFLNFRNNRFLLLKNLTQKEYQKISFIRNCLDIIAAFVAILKG
                     NFQEAKAIIHALKDFQKQQSLYLKKRTNNELLIRLSAVDVPNKKGIYSKSILYQYFVK
                     GKKKFSEIFH"
     gene            complement(27634..28791)
                     /locus_tag="PLS16_00140"
     CDS             complement(27634..28791)
                     /locus_tag="PLS16_00140"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HNJ59513.1"
                     /translation="MNKNWIIYRIYLFKIFAILFSLFVPYNFLFAQDDLIFEVHQDGL
                     NAYDTVISVLNFVDTNPIEEPYVGKLKLAKPYKFFENSPELNKRRVALVAGLQGGLYA
                     AANTWWSTAWYSKTEKSKFHTFNDWGDFNNIDKLGHGMSCYFESKWTYDLFKWSGVKE
                     KHAIWIGMLTGNLWQLSIEVHDGFQKKWGFSWGDMGMNLTGSLLFGIQQYWWHEQRVM
                     MKMSSFPINYTKRYKDTEIKQRADKLFGTSFAETMLKDYNAMTIWFSVTPGSFIKEKK
                     QNFARALQVSFGFGAGGMLGGYENRWSKNDLSGDNILDGTDPADIIDRSDIERYRRYY
                     ISMDIDWTKIPVKRKWAKTCLGIINIIKLPFPALEFNTASSHKVRWHWLKF"
     gene            complement(28784..30196)
                     /locus_tag="PLS16_00145"
     CDS             complement(28784..30196)
                     /locus_tag="PLS16_00145"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59514.1"
                     /translation="MKNNNIKFGTDGWRAIIAQEFTTDNVARVAYATAKWVKSIDENA
                     SVAIGHDCRFAGPLFVETAIKILCQEGVKVFAHKGICATPMLSLACVNYKTTAGIVIT
                     ASHNPPSYNGFKLKSNYGGPTIPADIAIVESLIPESIEIPKTNLDDFQKQGLLEYVDM
                     ETDYFNHVEKNFDMEAIRNSGMVFGYDAMYGAGQNIARRLLPNSLVYLHCEHNPGFDG
                     QAPEPIHKNLLEFSELMKNSSDLHFGFANDGDADRIGCYDGEGNFVDSHHILLLLIHY
                     LYKYKGEKGKVVVSFTVSNKVKKLCEAYGLECIVTPVGFKYICEIMVNDDVLVGGEES
                     GGLAIKGHIPERDGVWIALVLMEFMAKTGKTINELIQEVYEIVGSFEYDRYDLHISEE
                     IKQNVIKNCTEDNYKNFGKYNIVGRETIDGWKYFLNDEDWTMIRASGTEPVLRVYAQS
                     NNRANVIDVLESVKKVLLSE"
     regulatory      30277..30374
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            30423..32861
                     /locus_tag="PLS16_00150"
     CDS             30423..32861
                     /locus_tag="PLS16_00150"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ59515.1"
                     /translation="MKTRIVLSSALVCLANILFSQQIFKGKIENEAKQPLTNVAIFNT
                     QDSSVIYSDEKGNFEIEITKKTPISLSLDAYTSIYDSIYPEQQEKTFTLYYNLQQLEE
                     VQVMATRAGNKTPTSYTNLSTEKIQQQNFGQDLPYLLNGTLSTVVSSDAGAGVGYTNM
                     RIRGVDQTRTNVTINGIPINDAESQVVFWVNMPDLASSTENIQIQRGVGTSSNGAAAF
                     GASVNISSNKIKQKPYLELDNSFGSFNTLKNTIKIGTGIFKKHFAIDVRLSNIISKGY
                     IDRSSSNLKSYYVSGIWLNNKSILKFITFGGREITYQAWNGVPESRIKNDQEGMLAYA
                     ERNGLSETENDNLLNSGRTYNYFTYKNQVDHYKQTHYQLHFNHSFSNVLKLNVSLHYT
                     RGKGYYEEYKNQQDLEDYGIEPISIKDTSILNTDLIRRKWLDNHFYGMVFNLNYTKNN
                     LDLVYGGGFNQYLGKHFGDVIWARYTSNSEINHKYYDNDASKIDFNNYLKLNYQFKKN
                     TLFADVQLRVVHYAFNGFADLSGNTNENMQKVNYVFFNPKAGWMYHWNNHHNTFISIA
                     IANREPIRDDFIDNGQGKQPRHETLYNLEFGHKFQKNKWALNANYFMMYYKNQLILTG
                     KINDVGGYTRTNANQSYRTGFEIETSFQVFKWATILAGGTYSFNKIIDFTEYVDDYDN
                     GGQVAIQYKNTDLALSPNLIASWGLNLQPVKDFNIYINGKYVGRQFLDNASDKARSIN
                     GYYTMDLSLAYQLNLKKIKGIEFSVQMNNILNKKYENNGYTYSYIYGGERVKENFYFP
                     QAGIHAMGRISIKL"
     gene            complement(32939..34030)
                     /locus_tag="PLS16_00155"
     CDS             complement(32939..34030)
                     /locus_tag="PLS16_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HNJ59516.1"
                     /translation="MSNLEIKPIKEWGFNFKKPIVISGPCSCETEDQMMETARRLGNQ
                     NIDVLRAGIWKPRTRPGMFEGIGKEGLSWLVNAAKEIGKPTTVEVATAQHVEDCLNAG
                     IDILWLGARTTVNPFSVQEVADALKGVDIPVLVKNPINPDLQLWMGAMERLNKVGITR
                     VGAIHRGFSTYEKTKYRNKPMWELPVELKRLVPEIGIIVDPSHICGNRELLAYVSQKA
                     LDLDFDGIMLESHRDPDNAWSDAAQQITPEQLGELVANLVVRHSKLDDYEEDKLEALR
                     ANIDRIDNYFLELMSERMDIARAIGQFKKDNGITILQTGRWDEIVNDRVKKGAKKELT
                     EEFVKEMFEAIHQESIRNQTRIMNTITAE"
     gene            complement(34095..34391)
                     /locus_tag="PLS16_00160"
     CDS             complement(34095..34391)
                     /locus_tag="PLS16_00160"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNJ59517.1"
                     /translation="MILEAFTLKIKAEQTVKFEENFLKASEIINKSKGYIKHELHACK
                     EIKNQYLLMIYWETYENHMIDFRNSEGFKEWRALLQDFYDELPTVLHYDRVVYP"
     gene            complement(34398..35240)
                     /locus_tag="PLS16_00165"
     CDS             complement(34398..35240)
                     /locus_tag="PLS16_00165"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HNJ59518.1"
                     /translation="MKVVVFGMGLIGGSVAKDLKIRNFATEIIGVGRTDATNQKVLDL
                     KLVDRILPRDEAIEIADLIVLTVPVNALIDELLFVLDSIKSHQVVTDMGSTKGAIVDA
                     VQNHKNRSRYVASHPMAGTENSGPTAAINNLFDHKVCIICDKEKSDSDAVELVEKMYE
                     ALKMNLKYMDAHQHDIHAAYVSHISHISSFVLAATVLEKEKDESAILEMAGGGFESTV
                     RLAKSSPEMWTPIFIQNKTNILEVLDTYIEKMYSFRNYINRGKYDELNQFMKDANQIK
                     KILK"
     gene            complement(35279..36448)
                     /locus_tag="PLS16_00170"
     CDS             complement(35279..36448)
                     /locus_tag="PLS16_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNJ59519.1"
                     /translation="MIIEPANKLAHINEYYFSAKLKEIAQLRAQGKSIINLGIGNPDM
                     PPSEATINALVETAKQPNTHGYQSYIGTPELRQGLASFYQATYNVTFNSNSEVLPLLG
                     SKEGISFISNAFLNPGDKVLIPNPGYPTYTSATYLAGAEPIFYDLDEQNNWLPKLDSI
                     DEKILNEAKLMWVNYPNMPTGSEGNIAYFEKLIAFAKKYKILVCNDNPYSLVLNNERP
                     MSIFHADGAKEVCIELNSLSKSHNLAGARIGMIVGDKAYIDTILKVKSNVDSGMYLPV
                     QAGAIAALTNNQEWHNTRNEVYKKRRTIAYQILDTLNCSYTKEQVGMFLWAKIEDKYN
                     NVEDLTERILHEADVFITPGFIFGTNGARYIRISLCSTENDLQEALKRIEKLFNI"
     gene            complement(36468..37313)
                     /locus_tag="PLS16_00175"
     CDS             complement(36468..37313)
                     /locus_tag="PLS16_00175"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNJ59520.1"
                     /translation="MKTKIAIQGFKASFHEIAAIKFFGEEIETVECGSFTKLFDVMAN
                     QQADFAVCAIENSLAGSLLNNYANIGNSNLEIIGEVYLRIGMNLMALEGQKIEDLEEV
                     HSHPIALLQCRNFFRNYPNIQIVESSDTALSAEEIYKNQIKKRGAVASKRAAKINHLE
                     ILAEDIHDNKRNFTRFLILEDKSKVNSRSEFINKASISFRAFHTPGSLAKILTVIGEH
                     QINLTKIQSLPVIGEEWQYYMYADLEFEDKNAYQTMKKQIEPLCKELKILGEYERGKK
                     IIVSE"
     gene            37560..38408
                     /locus_tag="PLS16_00180"
     CDS             37560..38408
                     /locus_tag="PLS16_00180"
                     /inference="COORDINATES: protein motif:HMM:NF019436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59521.1"
                     /translation="MLNFDTVTNLAPFEKLAYCNTQYPTILVSGLGFQDQNKVTNYWG
                     KVPEYLKLHGADVYLAKQHAFLSIPDNAIRLKYRILDILEATKKDKINIIGHSKGGLE
                     ARYVAGKLGIHGQIASITTLGTPHQGAHLADIVLGKIPIPNFAMAQLTNIYAKILGDS
                     QPDSFRAVLQVSTQQLKKFNAEVPDIDSIYCQSYAGHINKDYPGTMWRTLAGILKPYD
                     GKNDGLVGVESAKWQNYKGLIATEDAPSVSHGDMIGFAQGIGEKKFNARAFIAYLLHD
                     LKERGC"
     gene            38564..38770
                     /locus_tag="PLS16_00185"
     CDS             38564..38770
                     /locus_tag="PLS16_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNJ59522.1"
                     /translation="MKKNIGKTDRIIRFSVAIVIALLYFLGLIPGIAATILGVLAVIF
                     IITSFISFCPLYLPFGISTRRKDA"
     gene            complement(38849..39262)
                     /locus_tag="PLS16_00190"
     CDS             complement(38849..39262)
                     /locus_tag="PLS16_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HNJ59523.1"
                     /translation="MYPKQMTDPMEAELTQNGFQALKSADEVTEALSSKEGTTLLVIN
                     SVCGCAAANARPAAIVSSKNEKHPTKLVTVFAGVDSEAVAKAREFTMPYPPSSPSMAL
                     FKDGNLVHFLERHDIEGYTAQAVAQKLVNAYNQFC"
     gene            complement(39327..39659)
                     /locus_tag="PLS16_00195"
     CDS             complement(39327..39659)
                     /locus_tag="PLS16_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HNJ59524.1"
                     /translation="MIVEKMNKTFIEVKNDVIELLHEIYDPEIPVNIFDLGLVYEAEV
                     DMENNVHIVMTLTSPNCPVAESLPKEVEDKIKEIDGVNNVKVEVTFEPPWDQDMMTEE
                     AKLELGFL"
     gene            complement(39661..40083)
                     /locus_tag="PLS16_00200"
     CDS             complement(39661..40083)
                     /locus_tag="PLS16_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HNJ59525.1"
                     /translation="MATINDIQDEIIEDFSIYEDWTDKYEYLIDFGKSLSILDEKYKT
                     EETRINGCQSKVWLYAEKQADKVVFFADSDAIITKGIVGLLVKVLSGHTPEEIAESDL
                     YFIDKIGLKEHLSPTRANGLVSMIKKIKTYGVAFQAIN"
     gene            complement(40090..41307)
                     /locus_tag="PLS16_00205"
     CDS             complement(40090..41307)
                     /locus_tag="PLS16_00205"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HNJ59526.1"
                     /translation="MLDIQKIRADFPILSEKIRGKQLVYLDNGASTQKPKVLIEAEQY
                     FYEHQYANIHRGVHYLSQIGTDLYEASRKTVQQFINAKHDHEIIFTKGTTNSINLVAY
                     TFGRKFIQARDEIVVTEMEHHSNIVPWQILCEEKGCILKVIPLQKDGTILLEDAARLI
                     TSKTKLVSVVYVSNALGTINPIQELIALAHSKGAKIMIDAAQAIQHIPIDVQALDCDL
                     LAFSAHKLYGPTGVGILYGKEDILNAMPPFEGGGDMIENVSFAKTTFNALPFKFEAGT
                     PHISGCISMSVAIKYIQDLGLQKIMEYEHSLLEYATERLNAINGLTIYGTAKDKSSVI
                     SFLLEGIHPYDVGVLLDNQGIAIRTGHHCAQPVMEYFNIPGTCRASFALYNTKEEVDV
                     LVEGILRAKKMLS"
     gene            complement(41307..41861)
                     /locus_tag="PLS16_00210"
     CDS             complement(41307..41861)
                     /locus_tag="PLS16_00210"
                     /inference="COORDINATES: protein motif:HMM:NF017970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HNJ59527.1"
                     /translation="MRAKQIQQLLKSQEKDIQSLHDIVMKSIEEEDLIMMNLKNKSVE
                     QLSRAQQLTDSIAKFGGSWTFIISFCFILFVWILFNVLAITKYRFDPYPFILMNLVLS
                     CLAALQAPIIMMSQNRMEEKDRQRAENDYIINLKSELEIRSLHQKIDLLLENEIKLLF
                     ESQAQQLDLLNSLRKEFIVRKENK"
     gene            complement(41864..43081)
                     /gene="sufD"
                     /locus_tag="PLS16_00215"
     CDS             complement(41864..43081)
                     /gene="sufD"
                     /locus_tag="PLS16_00215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HNJ59528.1"
                     /translation="MSLEILKNIQLKDKNQQQSLENAISIGSPSMKHEEWKYTYYNKI
                     FQNPFEVNIDTKNENQKIDAELVPLLQEEKYKLVFVDGQFIAELSYFPQSQKFSFWNN
                     NNVTESDSKDFLLELNNALVSDGVFVEIQDGFIEEENIGIYYIATKPNQIFNLKNKVK
                     IGKNVQVKFSEYFINPNNISTYYNTVSQIDIDKNSIVVWDKIQDGGELYYLTDNTFVK
                     QEKSSVFTINTITVSGNIVRNNLVIDVNGEYAENHMNGLYLLHGQAHCDNRTLVRHNE
                     PNTNSNELYKGILADLATGVFNGKIIVAQKAQKTNAFQSNRNLLLSNDANIYTKPQLE
                     IFADDVKCSHGATSSQIEDSELFYLQARGIGKEMSRSLLTLAFAGEVLDKLSDRNKQF
                     VEDKIFTTLGINI"
     gene            complement(43139..43882)
                     /gene="sufC"
                     /locus_tag="PLS16_00220"
     CDS             complement(43139..43882)
                     /gene="sufC"
                     /locus_tag="PLS16_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNJ59529.1"
                     /translation="MLQIKNLHVSIDGREILKGFSLEINKGEIHAIMGPNGTGKSTLG
                     NVLAGKDGYEITSGEILFNGENLLELEAHERAQKGLFLAFQYPVEIPGVSNANFLKAA
                     VNEQRKAMGLEPIAPGEFLKMMREKMELVEMKKDFISRSVNQGFSGGEKKRNEIFQMA
                     MLNPQLAILDETDSGLDIDALRIVANGVNKLHTPENAFIVITHYQRLLDYIVPDFVHV
                     MFDGKIVKTGDKNLALELEAKGYEQFELA"
     gene            complement(43984..45432)
                     /gene="sufB"
                     /locus_tag="PLS16_00225"
     CDS             complement(43984..45432)
                     /gene="sufB"
                     /locus_tag="PLS16_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNJ59530.1"
                     /translation="MSNTQDDILEEVVNADYKYGFVTDIEQEFAPKGLSEDIVRFISA
                     KKEEPEWMLEWRLKAFREWQKMTEPHWANVHFPKVDFQEIIYYAAPKVKPQLDSLDQV
                     DPEILKTFEKLGISLDEQKRLSGVAMDVVFDSVSVKTTFQETLKEKGIIFCSMSDAIK
                     NHGELVKKYIGTVVPQKDNFYAALNSAVFSDGSFCYIPKGVRCPMELSTYFRINSAGS
                     GQFERTLLIADEGAYVSYLEGCTAPQRDENQLHAAVVELIAFDNAEIKYSTVQNWYPG
                     NKDGVGGVYNFVTKRGMCKGNSSKISWTQVETGSAITWKYPSCILAGDNSIGEFYSVA
                     VTNGFQQADTGTKMIHIGKNTKSRIVSKGISAGKSQNSYRGLVKIHKKAEGSRNFSQC
                     DSLLMGDQCGAHTFPYLEVSNKTAKVEHEATTSKIGEDMLFYFQQRGIDEETAVAMIV
                     NGFCKEVMDHLPMEFAVEAKKLLEVSLEGSVG"
     gene            complement(45595..46902)
                     /locus_tag="PLS16_00230"
     CDS             complement(45595..46902)
                     /locus_tag="PLS16_00230"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNJ59531.1"
                     /translation="MKFFHKLLISLILFGASSSYAQEVMTPEEAVAYAVSKNFDIVLA
                     KNDAEVAKVLNNKASAGMLPKINLNTGDVFNLNNLNQKFASGQNVKQNWVAVNSLNAG
                     VSMDWTIFDGMKMFATKDRLAALQSLGEIQLKNQIQDVAAQTLNAYYNIVRQKQQIKA
                     AKELIGISEERVNLSQKKLDVGYSDKTPLLQAKVDLSTQQINILKQETLLEQAKINLN
                     QIIGRNADEPFDVIDNIEVNYQPNLQTIRDTTTSTNYLLAAAKKNIEIAQFQRKEINA
                     QRLPQINFTSGYNFVQNNNKAGFQLFNRVYGPSVGVSAKIPIFNGGMVKKDLQAASIN
                     IAKQQIQYDKLKQDIDAGIVKAYKDFELSEKMLQLNEENVQIAAENVQITMERYRLNQ
                     STSLDIKQAQSSYEDALYNVILARYNSKVAEIELKRIINNLLD"
     gene            complement(46913..50002)
                     /locus_tag="PLS16_00235"
     CDS             complement(46913..50002)
                     /locus_tag="PLS16_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNJ59532.1"
                     /translation="MGLPTLSLRRPVFAVVMNIAIILFGIIGYTFLGVREYPAIDPPV
                     INVRTNYTGANADIIESQITEPLENAINGVQGIKNISSSSNQGSSNITVEFELSADLE
                     TAANDVRDKVSQAIRLLPQDIDAAPVVSKADANSDAIISMTIQSDDKNVLELTDYATN
                     VLQQRLQTIPGVSTIQIWGQKKYAMRLWFDPQKMSAYGLTAVDIQNALNKENVELPSG
                     KLPGANTELTVNTIGRINKPEEYDNIIIKDVGGQTVRLKDVGYAVLGAENFETSLKEA
                     SVSMVALAIVPQPGANYVEISDEFYKRMEQIKDEVPKDIKLNIALDNTVFIKNSISEV
                     KETLIIAIVLVILIVYIFFRDTVMALRPLFDIPVSLIGAFFIMYVFGFTINVLTLLAI
                     VLATGLVVDDGIVVTENIYKKLEKGMPKMQAAIEGSEEILFAVLATSITLAIVFLPIV
                     FMQGFVGSLFMEFGVVVAGAVLISAFVSLTLTPVLNVWLGGKEHKNSWLYDKTEPFFK
                     GLDDGYKEMLESFMKIRWIAVVVIGICFAMIYFISKNLQSELAPMEDRGAFRVMVSAP
                     EGTSYTAMQDYVDRMTDFVVDSVPEQSIVLSVTSPSFAGSGAANSAFMRVALKDKDQR
                     VRSQNDIAQYLQRNFSQFNLGRAFVIQDQTIAVGSGRFGMPVQFVIKNFDFEKLQNII
                     PKFMDEVAKSPVFVGYDVNLKFNKPEVDIIIDKDKAYSMGVSVQDIAQTLQFSMSGRR
                     FSYFLKDGKQYEVIGQALYDLRNKPDNLKDIYVRNNSGALISLDNIITVREIAKPPQL
                     YHYDRYKSATVSSALAPGYTIGDGIDEMNKIAKKLLDDTYSTALSGSSRDFAESSSNT
                     MYALILALVLIYLVLAAQFESFVDPFIIMMTVPLALAGALLSLWIFDQTLNIFSQIGM
                     IMLIGLVTKNGILIVEFANQLREQGMNKLEAIIHASQMRLRPILMTSFAMALGAVPIA
                     VALGKAATSRVSLGIVIIGGIVFSLILTLFIVPAIYSFMSRERKENEADLVLKNQSH"
     gene            complement(50052..51146)
                     /locus_tag="PLS16_00240"
     CDS             complement(50052..51146)
                     /locus_tag="PLS16_00240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNJ59533.1"
                     /translation="MKKGKLIFGLSILAIIAILAYVKIGKNKEAKAKQGSLRAMGGKM
                     PALAVDGFLVEYSKLADNISANGNVLAQDEVQLQPEVSGRVTYLNIKEGAFVAKGTLL
                     LKINDADLQAQVAKLKTQVKIAQTNFNRLAELLKIKGVSQAEYDAAENTLNNIKADMQ
                     ILQAQIDKTELRAPFSGKLGLRNISLGAMVSTTTIVTSLQNTSQLKIDVAVPEKYASV
                     LNLGDVMSCKIAGINEPVLAKVAAIDPVVDEMTRNLKVRAVLLSPNAKIVPGSYVKVD
                     LKLKEIPNSIVIPSGAIIPDDKSTKVVIVGDSSKAQFVNVEVGVRTENSVQILSGLKL
                     GDTVLTTGLLQVRPGMEVKIRKAVSNSTIQ"
     gene            complement(51167..51766)
                     /locus_tag="PLS16_00245"
     CDS             complement(51167..51766)
                     /locus_tag="PLS16_00245"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNJ59534.1"
                     /translation="MTIQTRRDKEKVEMRQLILDVSKAIASEDGWQNLTIRKICQKIS
                     YTAPVIYQYFESKEAILLALQEDGFNQIKQQFELVDEQHKIASKRLFEYAMVWWNFTK
                     ANPELYQVMFSLQGVVCNQNGCQRLEEILEIYKLAFSSLNDGALKSKQTNLEFCDNYI
                     AIIHGYIDIKLIDKIKSKDRYNDQSYKNALMRFIDSIKN"
     gene            51954..52937
                     /locus_tag="PLS16_00250"
     CDS             51954..52937
                     /locus_tag="PLS16_00250"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNJ59535.1"
                     /translation="MLSNIYHRIYDKVQYKFSLIFIGIYIVIYNYQEINIGILFYLFI
                     AVITSIGIAGLGYILNDYKDLEDDIKNNKRNIFIQLNEKEKIIVIVLFLCLAGIPWLY
                     FPFDIISALLILLELILFYIYAFPPLRLKEKGFWGILCDSLYAQVIPCSLAAYTFYKI
                     QTPKQNFSIYFILIVLLWLFIIGIRNILIHQIDDFENDQNTKTKTFATNIGKKQTMKI
                     VYNSILPLEIILFTILLYQLPQNGLFIIALYIIYCIAIFLINKEHFRKHFAHYVNQRL
                     LNEFYEIYLPVLLLIIYYISGIHSLYIPIFHFVLFAPIYLKSFLAFTKKYF"
     gene            complement(52934..53554)
                     /locus_tag="PLS16_00255"
     CDS             complement(52934..53554)
                     /locus_tag="PLS16_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59536.1"
                     /translation="MNKTYVIGVSGGSGSGKTTFVRNLRKFFSEDEVCLLSQDNYYKK
                     REEQVVDKNNHQNFDLPTSFRKDEFEKDVKRLLNGETLELMEYTFNNDLATAEKIIYK
                     PAKVLIIEGLFVYHFREIEALMDLKLFIDIPSYLELTRRIVRDQAERNYPLDDVLYRY
                     ENHVYPAFEKYIFPYKADCDIVINNKTRESLEHSIEILKNHIKTKL"
     gene            53625..54530
                     /gene="folD"
                     /locus_tag="PLS16_00260"
     CDS             53625..54530
                     /gene="folD"
                     /locus_tag="PLS16_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HNJ59537.1"
                     /translation="MQLLDGKKTAASIKDEIKQTVDAIKLQKKRVPHLAAILVGDSTA
                     SKTYINNKIKSCKEVGFKSTLLHLEKDITENELIAQINNLNNDNSIDGFIVQLPLPKH
                     INEERVLLSITPEKDVDGFHPINLGKMMIGLPAFISATPKGIVEILKRYHIQTSGKHC
                     VVVGRSNIVGTPISILLGKKSEAGNATVTLCHSHTKDLKTYTLQADIIIAATGKPNLI
                     TADMVKDGVVIIDVGINRIDDNSERGYHLVGDVDFKNVSKKSSYITPVPGGVGLMTVA
                     SLLQNTLAAYQAAYYPKDFIEENLL"
     gene            54530..55018
                     /locus_tag="PLS16_00265"
     CDS             54530..55018
                     /locus_tag="PLS16_00265"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HNJ59538.1"
                     /translation="MNKDLIFKNAIGAALIYIILKVVTLAIGIEMQYPIDWVIFAIIV
                     GLFIFFGKLYSQSINDESNMGDILLHGFVTSLVYIFFISIFTVIYVKYIDVNYYQKIF
                     DMAEKELQNYSMEPEKMEAMKELSKKYMTIPYLLLSTFLSNIILCNIGNFIGAVIFRN
                     KN"
     gene            55034..55927
                     /locus_tag="PLS16_00270"
     CDS             55034..55927
                     /locus_tag="PLS16_00270"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ59539.1"
                     /translation="MNIHTLALQNFPDYELIDCGNFQKLERFGQYITIRPEPQAVWDS
                     KLNIDEWKKLAHVSFVSKSSSSGEWQKLKTMPDNWNIKYQFRDKKSIQFNLALTKFKH
                     VGVFPEQCVNWDFIYDRSKQIIQQNNTCNVLNLFAYTGGASLAARTAGADVWHVDAIK
                     QVVSWANRNMESSNLDNIRWVVEDALKFVKREVKRGKKYQGIILDPPAFGHGPNGESW
                     KLENQINELLFDVIKLLDEKHQFMVLNVYSLGFSSLIVENLVQSNLDKNGVKISNMEI
                     GEIYIPSQQKQKLPLGVYCRF"
     gene            complement(55949..56284)
                     /locus_tag="PLS16_00275"
     CDS             complement(55949..56284)
                     /locus_tag="PLS16_00275"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNJ59540.1"
                     /translation="MEKLELNSDQIQKAAELLKAVAHPLRLKIVKLINEKGEVNVNVI
                     YNTLKIEQSITSQHLKMLRGVEAVDTRRDGKKIYYTLNAERFNILSRAVAVVDEYKSK
                     APAKKKATK"
     gene            complement(56397..57086)
                     /gene="tsaB"
                     /locus_tag="PLS16_00280"
     CDS             complement(56397..57086)
                     /gene="tsaB"
                     /locus_tag="PLS16_00280"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNJ59541.1"
                     /translation="MSVNIICIDTALSTCSVALANGTNLLGYSECLLPNTASEQINEL
                     INEALSQANLKLSDMQAIAISNGPGSYTGLRIGAAVAKGIAYALNIPIIALPTLAIMT
                     NKAIISENISDFLVVPNIDARRDEVYFAVYNSKLEELYKAQPCILDDDFLSFFENGNQ
                     YFFIGNASEKIHIYLQQKLLNHKFIFHQDFEYTAKDMIELANHYFQLTNFVDVAYFEP
                     SYTKPFYSTKK"
     gene            complement(57086..58753)
                     /locus_tag="PLS16_00285"
     CDS             complement(57086..58753)
                     /locus_tag="PLS16_00285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNJ59542.1"
                     /translation="MKRFFVLLFSLFLSLSILAKENTPSTTSNNKYFDMAKNIELFTN
                     VYKELNNSYVDDIDPNTLMTNGINKMLENLDPFTNYITGADIDEYQMQTTGKYGGIGA
                     RIGRINNEVMITEPYEGFPAQKNGLEPGDVIIEIDGKVCKDYNSSDVSELLKGEPGTS
                     LTVKVRKAISNEIKTYTFNREEIKINSVAYSGMLDNNIGYIKLISFTENASKEVQDAL
                     LNLKKNPNLKGVVFDLRGNGGGLLMEAVNICNAFIDKGNIVVSTKGKVPENNYDYTTQ
                     NSPVDLQIPLAILIDENSASASEIVSGSIQDLDRGVVIGQNSYGKGLVQVTKPVGYKS
                     KLKLTISKYYIPSGRCIQRIDYSHRDENGKASALPDSLRSVFKTKNGRLVKDGAGIEP
                     DIKTEEQVMSPITIALYVNNLIFDYATQYKYDHKVLRDSTKFSMNEEEYKQFVKFLEG
                     KKYDYTSKTEETLDKLNDLIKDENYSAEIKSTLGLLHDKIDADKKQDVFKYEQQIKKL
                     LEDEIVARYYFEKGRIANSLINDNDVAKAKEILLNYIQYKEILSAKK"
     gene            complement(58835..60190)
                     /gene="accC"
                     /locus_tag="PLS16_00290"
     CDS             complement(58835..60190)
                     /gene="accC"
                     /locus_tag="PLS16_00290"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNJ59543.1"
                     /translation="MKITKILIANRGEIALRVIRAAKELGIKTVAVYSTADKESLHVK
                     NADESFCIGAPQSKLSYLNQDAIIQAAILSKADAIHPGYGFLAENEGFARKCAENNIK
                     FIGPTPEQIASMGDKITAKETMIKAKVPVVPGSEGLITDVKKGIQLAKKVGYPIILKA
                     TAGGGGKGMRIVWEESEFEHNMQQARVEAGASFGNDGVYLEKYIEEPRHIEVQVAGDQ
                     FGNACHMSERDCSIQRRHQKLVEESPSPFVDDKLREQLGVAAVNAVKAIRYEGVGTIE
                     FLVDKHKNFYFMEMNTRIQVEHPVTEEVTNLDLIRLQILIAAGEKIEQSVYAPSGHAI
                     ECRINAENPLDNFKPSAGKIINYIPSGGIGVRIDTHVYSGYSIPPFYDSMIAKLIVKA
                     PNRQQAIEKMKRALAEMKIEGIHTTIPFHQKLMNNKDFVAGNFTTKFLEEHTFDYEDD
                     V"
     gene            complement(60382..>61283)
                     /locus_tag="PLS16_00295"
     CDS             complement(60382..>61283)
                     /locus_tag="PLS16_00295"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNJ59544.1"
                     /translation="EDFTTFMQKQHAIYDNLYSNPQYYFMKKLSEINYNNNIRRNLPT
                     KETLDQVNYANAFKFYNERFWNAADFTYYFVGNIDAKQIDTLITKYIASLPGDSTKRE
                     MWKDPNVEKPKGKVETNLKMGNTPKTFVNINEHGTLNYNDAEALKFNAMVKVLSIVLR
                     EELREEKGGVYGVSVQGVVSKIPKEKFAINISFNADPPKAQELIKATYDEIDDFVKNG
                     TTLEKLNKVKETYKREFETDQKENDYWIGKMVYADKYNWDIHKLNQYLQAIDTLSLED
                     IKQAATKYLTTDDVIQVTLSPVQ"
BASE COUNT        20882 a        10402 c         8369 g        21630 t
ORIGIN      
        1 tctttgctac ccacgaccct actactttaa acagacaagg caacgatgtt ggcacacaat
       61 atagaagtgt aattttctat cataatgaaa cgcaaaagaa acttgcaact gaagttattt
      121 ccgaattagc tttaaatcaa atttacaaac aaccaatagt tactgaaatt gttcctattt
      181 ctaatttcta taaagcagaa gattaccatc agaattattt tgaagaaaat agcacttcca
      241 acccttactg tgaaattgtc attcaaccaa aagtggagaa atttgaaaag ctatttaaga
      301 atttattgaa agacaaataa gcattaaaaa aaaggctgtc ctaaatattt tgaacagcct
      361 ttttttatta ttttgataat gttctaaaac attatccatc tgctttaatt tacgactttg
      421 tcattcaatg tatcttgatc atttaattga atatactttt tattctttct taaacgcata
      481 ttcaaaaact caaccgacaa accaaaagcc attgctgtat aaatatatcc tttattgata
      541 tgttggtgga acccttcagc aattaaaata attccaatag ccactaaaaa ggctaaagct
      601 aaaatttgaa gcgtagggtt tttatcaata tattcagcta caggatttgc aaaaatcatc
      661 ataatgacca ttgaaataat cactgcaaaa atcattatcc atacattgct cactaaacca
      721 attgccgtta aaatagaatc tatagagaat acggcatcta tcaataatat ttgaacgata
      781 actgtaccaa atgaagttac tgctttactc actgtacttt tttggtttgc tccaatttct
      841 tgctcaatgg aatcttctaa tttatgatgt atttcagaaa tactttttgc aattaagaag
      901 aatccgccaa gtaataaaat caaatctttt atactgattc caattggttg gtcattatct
      961 tcaataaatg gaatgtaaaa caaaggattg gtcaatttta ttatataagt tattccaaat
     1021 aataaaccta ttctaaaaaa cattgccaaa agcaatccta tttgtcttgc tttaggttgt
     1081 tgggctttgg gtaattttgc cgaaactatt gaaataaaga taatattatc aattcctaag
     1141 acaatttcaa gaaatgctaa agttagtaaa gatataataa ctgaaagatc taataaattt
     1201 ggaaatacat caactaatag cattttttat tttttttgca aaaataattt ttagtcttaa
     1261 caaaaagaac tgtacgattc tataattaag aaaaaataac ttatttcatt ttattggcta
     1321 attggccaca agctgcgtca atatcttttc ctctacttct tcgaatgttg gcaataactc
     1381 tttgtgaact caagtattta ctaaaagcct cttgtttttc tacagatgaa cgttgtaagt
     1441 ccacaccttc cacggtatta tactcaatca aatttacttt tacattagga atttgtttgc
     1501 aaagtgccac taaattttta gcatcttcaa tgctatcatt gacctctttc atcaaaatat
     1561 actcaaaggt aatttcactt ttagttttct gctcaaaata gataagtgcc tccattaaag
     1621 aagctatatt attttgttca ttaatgggca taatagcatt tcgtttttca tcatcagcag
     1681 cgtgtaatga aagtgccaaa ttaaaacgta cgccatcatc tgccattttt ttaatcattt
     1741 tggaaatacc agccgtcgaa actgtaatgc gtttggctgc catattcaaa ccatcttcag
     1801 aagtaatctt attgattgcc tccatgacat tgctgtaatt taaaagtggt tctcccattc
     1861 ccataaatac aatattcgtc aaatttctac ctaaactttc ttgtgcctga cgattaataa
     1921 aaaccacttg atcataaatc tcatctgctt caaggtttct ttctcttttt aaaaatcctg
     1981 tagcacaaaa tttacacgcc aaactacaac caacttgcga tgacacacaa gctgttgccc
     2041 tgtctgtact tggtatcaaa acaccttcga tgaaatgctt atcatgtaat tggaaagcag
     2101 ttttgatagt accatcattt gaatgttgtg cagaatataa ttttattgga agtattgtaa
     2161 atttttcagc taatttttct ctaaaaggtt tagataaatt tgtcattgct tcaaaatcag
     2221 taactgattt cttccaaagc cattcataaa tttgctttgc tctaaattta ggttcaccta
     2281 aatctttcat cgcagcctgc aattcatcta aagaaagttg tctaatattt tgcaatgcat
     2341 tcgtcatcat ctgacgcaaa gataatggaa agtttttgga gcaattagtg tattacatta
     2401 aatttagtaa tactaaaatt ttcgtcatca cttattttaa taaaatatgc accattagac
     2461 cacttactac aatcaatttg atgtatatta tttttattgc tttcaaaagt caaaacttgt
     2521 tttccattta tatctaatat attaatgtca atattatttt gaaactcaga agcaactaca
     2581 tctattgtca ataaattact aactggattg ggatatattt taattttttc tttaatttta
     2641 aattgatgat tagctgaaat tacttcagcc tcattcaatg catgtggatt aaaaaacttc
     2701 cacatttcaa cagcataatc aatatcatta ttttcttgat ttaataaaat atgctctgca
     2761 ttatttactt taaataattc aacgggttgt ttgcaattct gataaacata atgctcaact
     2821 gtcaaaccat cgttttttat atttggtata tctgtacgaa gtggtggctg attagaacat
     2881 tgattatttt tagtccaaac agacaacata tcttctacat ttaaaccgaa taaagtaata
     2941 taatatccaa cattcaaatc tgatgttccg tgaaaatgta aaattggtaa aaatctgcct
     3001 ggattacaat tatatatttt atttccaatt gttcctgcaa tagatgcaat agctgcaaac
     3061 cgttcattag attcacacgc catacgctga accataaatc caccatttga aaaaccaaaa
     3121 aggtaagttc tgtttttatt tattggatag ttggcttgaa gtgaatctgt aacagcattg
     3181 ataaaacctt tgtcatcaat tccagcactc ggatattgac cagtgtacca gctccagcat
     3241 tccagcctct tcctaagaaa aatttatcgt agccttgtgg aaataccaaa attatatctg
     3301 cggtatccgc aattttgcaa aaattataaa aagcacccga aaaatcagtc atattactac
     3361 ccaatccatg tatgccaaca actaacttat acagcttagt ttcatcataa ttatctggaa
     3421 gataaacacg atattctctt gttagattat tgtgtttgaa agatttattt aaccattctg
     3481 cataagtaaa attgacaacg aaaatgcaaa gaaataaaag tattttttta agcatagttt
     3541 tactttttaa attaattaaa attagaataa cttcttcatc aatataaaat taaaaaaaat
     3601 ctatcatttt tacaattgat tataaacaaa ttgcgttatt tcttgttcta cattttatga
     3661 gtttattaga gcaaaaagac aagcctgatg aaaaattaaa atcagaaaca aaaaagctgg
     3721 aatattcact tgatgtaagt gaagtctctg aagtaccaac aatacctaca tctttaatgc
     3781 cagaactcga agaattggtc gctaaaaatc caaatagatt aattggttgc ggtggataga
     3841 ttataaagtt attccaccta aaagttgaaa caaaaactga aatacttgta ttgttacgta
     3901 tattgaagct gccaaaacca acaaaaaggc tatcaatatt ggaattaagt aaggcaaaat
     3961 acttccttta tgttttcctt cttcaaaatg ctcaacagct aataccatat tacgtttatt
     4021 agccttataa taaaaatcga aaaaagtacc caaaacagga attgcaccca ctaaagcatc
     4081 aatacttaca ttccatatca ttttacccat tactttagaa cttgccccat ttttatacat
     4141 agcaaacatt aagtatatac ctattgcata accaactgcg gctcctccat aaggaattaa
     4201 attcaacaat ggatctatac caaacctaat attagtacca ggaatacgaa attgagcatc
     4261 catcaacttg acaaagcctt ggagtttttt taactcttca ggaattgggc gtttgtcaga
     4321 aataatttcc attgaaattt attttatcgt aatcatcggc aaactgatta aatgctcttc
     4381 ttcattagaa attgacatta aaccaatgcc agccattcct ccagatgctt gagcaatttc
     4441 tttagcaaat gattttaaag actttattaa tacagttaca aataatggat ctactttaat
     4501 taaaatatca tttaacccag ctaatacatc agaaagttct tgtgttcttt gttctgttcc
     4561 tttatcttta tattgattac taaaatcaaa caatctttga tcgtaatttt tatctactgc
     4621 ttcataatat ggttgtagta atgcatcatt gctatctttt cttacaatta cagagcttaa
     4681 accagctgca atttctttat catccatatt tccgtcagct gttccaatca aaagtgttat
     4741 aattgctgga gcatctaata ataattgtac ttcttcttct gctaaatgat taaattgagg
     4801 aattgccatt ttaaatatta ttttttgcaa aaatacatta taaagttaaa tatagtaata
     4861 aatttagctt tattttggga tttaatttta tttcacattt agaaaatggc tttcaaagta
     4921 aaagacctag acgatttaga aattgtagta aaagaattgt tacaatatac agaaaaatat
     4981 actatttttt gtttttttgg agaggttggt gcaggaaaaa cgacacttat tacgcagttt
     5041 tgcaaagcat taggcataga tgataaagtt agctctccta cctattccat agttcaagaa
     5101 tataaacatg gaaatcaaaa gatatttcat atagacttat accgattaaa cagtgaagaa
     5161 gaagctgttg atattggttt agaagattat ttatatagca acaatatttg tttgattgaa
     5221 tggccaaatg attttttaaa tctaatacca aagaaatttg tgaaaataga aattgaaaat
     5281 atggatggcg taagaaatat tagtattgaa gtgtaattat taggtatttt ttaataaatt
     5341 tcaactttca aaactggtga tattttccta aaagaatttc tttaaaatca caaaggtatc
     5401 agaattttta aatccactga aataaacaga gagaaagtgt tgcattagat catttctcaa
     5461 agtagcaaca tttagaaaca gtactcaaat gatattcatt ttaaggtgct atactatctc
     5521 aaagttatat ttattcctct caaattccat tatgtttttt acgtattgtt ataaaaaata
     5581 cgaaatacaa tattgcgtat aatatacaaa gaatacaaat aatacgcaat aagacatctg
     5641 tatatattta attagcttat aatttgttat aaatatttta catttttatt taataattta
     5701 aaattattaa ttttatttgt ctatataaat tattatatga tataacttga aaactcttca
     5761 tatatataac aacttctaaa atcgaatcga aactgactaa atattttgta aatttgtgga
     5821 caatcaacgc aataaagaca aaatggacaa taaattttct gatatcattc aaattattaa
     5881 aaaatctcga actaatgcta ttaaagctgt taattcggag ttgattaatt tatattggaa
     5941 catcggagaa catatcagtc taaaggtaga gcaaagcgaa tggggaaagt ccgttgtcaa
     6001 agaattagct gattttattc aatcgacaga acccgaactt aaaggttttt cagacaaaaa
     6061 tctttggaga atgaaacaat tttatgaagc ctacaaagac tttccaaaac tctcatcact
     6121 gctgagagaa attagttgga ctcataatct gactattttc tcaagatgta aaactattga
     6181 agaacaagaa ttctatataa agctaaccaa aaacgaaaat tacacctcac gtgaattaga
     6241 aagacagatt tcaagcagtc tttttgagcg aacaatgatt ggaaattcaa aactctcaac
     6301 atcgctgaga gaagcttcaa atgatctatc aaatacattt aaggatagtt atgtttttga
     6361 ctttctgaat cttcctgaaa ctcataatga aactgattta cagaaaggtt tgattaatca
     6421 aatgaaaaat ttcattctgg agcttggtcg agactttcta tttataggag aagaatacaa
     6481 agtacaagtt ggaagctctg atttctatat cgacctccta ttttatcatc gtggattgca
     6541 atgtttagtc gcatttgaac taaaagcaga caaattcaag cctgaacatt tagggcaact
     6601 taatttttat ttagaagtat tagatagaga tgtgaaacgt caaaacgaaa atccaagcat
     6661 tggaatacta ctgtgcaaag ataaagatag cgaagttgta gaatatgcac ttagtcgtaa
     6721 tctttctcct actatggttt ctcaatatga aacacaactt cctgacaaga aattattaca
     6781 acaaaaatta cagcaattat ttgaagatta aaatagccac atcatataac ggacagtttt
     6841 gcaagagagc aggagaagtt ctctgtttga aatattttag aatatttgaa gtgcagttct
     6901 cgcaagaaaa ttagtgataa tagaccgctc cctcgcaaag gtgcaaaaac gtcctaaaat
     6961 aggtttacac attaataact aaaaaaatgt gtaaaaattg aattagaaaa tattgatggt
     7021 gtaagaaata tcagtattga agctttataa ctgtatattt tagaataaat tccaactttc
     7081 aaagctgttg agattttgcc taaaagaaaa atcggcaaca caatacaatt gagttgccga
     7141 ttctgttttg gttaaatatg caagatttta ttgctcactc atagcttcat tttctatttc
     7201 tgacatttct tttctttttg aacctaacat taaaatttca ttagccacaa tttcagtaat
     7261 ttgacgtttg ttgccatttt tgtcttcata ttgtctatag gtaattttgc cattcaaggc
     7321 aatttctgaa cctttatcta aatagttctc acatactttt gcaactttac ccattgcaat
     7381 gatgttgtgc caataagtta cttgcttgcg ttcgccttta ttgtccacat aatactcaga
     7441 ggtagcaagg ctaaacgtta caatttgttt tccactttca aaggttttca cttcaggttt
     7501 gtttcctagg tttccgataa ggtttacttg atttctaatt gctgccataa taattaattt
     7561 gtaataagga cgcagacagt aaataaattc cataaataat tgttagaaaa atgaaataat
     7621 ttaataatgt agctatgaaa taatgaaata atttaggaat gtagtaatct actaatgtag
     7681 gtatgtaata atgaatttct tactacttaa tactttatac ttacttctca ttcattgtct
     7741 taataaggat ttgcaggatt aaatgattat caagtttttt tttagtagtt aagaaaatag
     7801 tgaaataata aattattgaa ttattgaatt aatttctcac tacttaatac tttctactta
     7861 tatttcaata aagtcttggt tcttggctct aactatctag gcactaaaca tcttgggtct
     7921 ataatctatc aaaaagaaat ttaccaagta atattcaact tcaagttaaa ccttcggttg
     7981 gtgaggtaat tgggaattgc ccattggttg ttgtctatat cttttatcca acgataggaa
     8041 agtgtattgt atatgcctat aatattaaag acttcaaaag atgtccaaat atttttaaaa
     8101 tgactgccaa aactatttgg ctttttcaag cctctttttt ctccatttaa taattcataa
     8161 gaaaacccaa tatctaatcg tcgataaggc ggcattttga gtacgtcttt ataacgctgt
     8221 ctatctggtg gaccaaaagg caaaccagta cctaccacta aattaaaatg aactttgaag
     8281 cttttatttt ttactaaata atcttgaaaa aataaattca taaaaaatgc ttggtcggtt
     8341 ggtcgtcgta tcattcctgg atagaccatt tgagaatctg gaacaccatt tatatcattt
     8401 actctataat aaaaatcatt gtctaaattt tctctagttc ttaaatagga aaaactaatc
     8461 caagaatcag taccttttac caattcgcca aacaaacgca catcaacacc agttgcataa
     8521 ccttttgctg catttgtagc aaaataacga atcctaatat cttgaatttc ataaggatta
     8581 aggttccata tatatttata gtaagcttca gttgtcaatt taaaattacg attaccccaa
     8641 gcattaaaat tatattcaga acccacaatt atttgtgctg ttttttgagc ttttacttgt
     8701 cgatttatac gaccatcaaa gcttctcatt tctcgataaa atggtgcttg ttgatacaat
     8761 ccagcagcaa atctaaaact tacatcagct tttaaatctg gttggtaaaa aacttgtatt
     8821 ctaggggata ctaaaatttc tttattgaca ttccaaaaat gataacggat acctgttgtc
     8881 aaacttaatt ttacagaatc ttgtcgaata ttccaagtat tttgaaagta gccatgtgta
     8941 cgcacagatg acaattgaat cgccgattta attctatctt gtgtggccac tgaattacca
     9001 taatatggaa ttgaaaaacc agcagaatcc actcttgtcc actcactcat aaggtcatca
     9061 atatgctcat attgtactgt attaccaaac tctagtagat ttttatttaa tgctactttg
     9121 gcggataaac ttgcattaaa aactgtagct tctaagctat ttctagccca attttgaaat
     9181 gtaccagcac ccaaactatt cttgacatta ccaaaattat ctttattggg gtcaatttca
     9241 atttcatcta aagcatagta gccgataata ttaaaactct ccacttcatt cattttccaa
     9301 aatgcagaat taaatttcaa actccatttt gcattaggga caaatttata agacaatcca
     9361 gccatagaat ttttaaatct agaattttca ccaccatcaa aatctactaa aaatcgtatt
     9421 aattgattaa aagtgcctgt ttttgtttct tgtaattcgg gcgtaaattt aaaatgattt
     9481 gcagcatggt aggctaaaat ctctaagtca tgtttattgt ttggcttata gtgtaaatca
     9541 atttgagcat caaaaaaatt gggattgtac tctcctttcc tatctaaaga acccaataca
     9601 taagcattgg ttttatagcg aactcctgtt aaataagtaa acttttgttt tttagaaaca
     9661 ccttccaaat gggcattaac acccaataaa ctcgccatta tagaacctcc atgtttttcc
     9721 ggtgttttgt attttacatt caaaactgat gacattttat cgccatattt tgattcaaag
     9781 ccaccagcag aaaaagatag ttgatctatt aaatcaggat tggcataact caatccttct
     9841 tgttgtccac tactggttaa gaatggctta tagatttcaa aatcgttgat atacaccaaa
     9901 ttctcatcat aattacctcc acgaactgaa tattgaccag tcaattcatt attggattgc
     9961 acaccagcaa aagtttttac aatagtgcca atatcattgg ttggattagg aaaatattct
    10021 acacttttcc cttttataat ttgtgtatta gtgtttgaat tacttttgga tataatttca
    10081 acagaatcta aactttcgat gtgttcaagc atttcaacat ctacctcttt tcgcatttta
    10141 ttgcttgaaa aaatttttat tgttttggta tcaaactgca aatgcttaaa actaacttca
    10201 caaaagttcc cttcaaaatc taatcttata gtataagtcc catcaatatt agaaacagta
    10261 acattattgg tattattttg aataatattg acattaggaa tcggattatt ttgattatct
    10321 cgaattacgc cacgtaaatg aatcgtattt tgggcaaaac ccaattgtaa tataaatagt
    10381 aaacagctta caataaaata ctttttcatc gtttgtgaat atatcaaaaa tagctaagat
    10441 ttgatatatt aaaacaaaaa aacagggcaa gaaatcttgc cctgcatttt tatacagatt
    10501 taaaacatta aatcagttca aaacttctat taacaaagtt agtcaatgcc tcacctttca
    10561 acatattttg tgataataat gccaagtcat acaattgttt caaatgcttt tcttgttgct
    10621 caccagtctc ttccaataat ttatgattga tagaatgatt cgtattaatt actaagttga
    10681 ttttttcatc catacctcca aacataaaag gagaacctcc acccattttt tgcatttctt
    10741 gcattcttcg cataaactcg tttcttgtaa ttacaatcgg tgcatcatta ggagaaagtg
    10801 cttttaattc aatatcaact tttgcttttt ctactgtatt ttcaaacaat actttcaagg
    10861 catcttcctg ttctttggac agtgcggatt ctacattttc atctttagaa attagattac
    10921 tcaaaatatc tgcatcaaca cgtttaaagt gtgttttctc atttttcatt tctaattgct
    10981 ggataaaatg attgtctatc attgtatcca actgcacaac ttcgtagcca taatctttag
    11041 cttgtttgat ataactgtgt tgttcttcgc tatctgatgt ataaagaatt actaaattac
    11101 catctttatc tgtttgatta gcggtaactt tctcttttaa ttcagcataa gtatagaatg
    11161 tattttctgt tgtttggaac aaagcaaaac tattacttct ttcataaaat ttctcatcgc
    11221 ttatcattcc atacttcaca aaaagaccaa tatctgacca tttttcttca aattgttttc
    11281 tatcagcttt aaatatttct tgtaatttat ctgatacttt tttagaaata tgttgattga
    11341 tttttttgac attaccatca ctttgtaaat aagagcgaga aacattcaat ggaatatctg
    11401 gagaatcaat tacaccgtgt aataaagtta agtactcagg aacaatttca cctacattat
    11461 ccgttacaaa aacttgattg gaatacaatt gtattttatc tttttgaact tcaaaattct
    11521 ttttcaattt tggaaaatat aaaattccag ttaagttaaa aggataatca acattcaaat
    11581 gaatccaaaa taatggttca tcaaaattca ttggatacaa ttgtctataa aaatttttat
    11641 aatcctcatc attcaaagaa gaaggcgatt ttacccaagc aggctctgta ttattgataa
    11701 tttcaggctc taaaggtttt tctgcttttt tatcttcttc agattgccat ttataaccaa
    11761 actgaatagg tatgggtaaa aacttacagt acttatttaa aagtgattta attctttgtt
    11821 cttccaaaaa ctctaaagaa tcttcagcta tatgtaaaat aatatcagtt cctctttctt
    11881 gcttttctac agtttctaaa gtataatccg tagagccatc acaaatccat tttgcagcag
    11941 cctcgtcttt atatgatttt gtttgaattt ctactttttc tgctaccata aaagctgaat
    12001 agaatcctaa tccaaaatga ccaattatat tttttgaatc tgtattttta agtttctcta
    12061 caaattctcc agctgatgaa aatgcaattt ggttgatgta ttttttgact tcatcagctg
    12121 tcattccaat tcctttatca gaaatggtga gtgttttatt ttccttatct aatttaactt
    12181 gaatggtagt gtctccaatt tcgcctttta cctcgcctaa ttgcgataag ccttttaatt
    12241 ttttagttgc atcaactgca tttgaaacca actcacgaag aaatatttcg tggtcggagt
    12301 ataaaaattt cttaataatt gggaagatat tctccgtctg tacattaatt ttgcctgtct
    12361 ccatattgtt attctatttt attaaaaatt tatcgctttt ttattttcaa ataacatacc
    12421 aaagcaagcc taactgacaa gttgtccgta aaagtattcc tctaaaaaat aaatatttaa
    12481 attgactatg ctgcccaaga atctctatcc aaactacgaa attgaatagc ttcagcaata
    12541 tgttctgttt gtatgtttgg agattttgct aaatctgcaa ttgttcttgc tacacgcaat
    12601 attctttcat aagcacgagc cgataagcct aatttattca ttgccacatt taataaattt
    12661 tgagaatttg catctaaagc acacatttca cgagtttgct tggtactcat ttgtgcatta
    12721 caatacacct cgccattttt aaaacgctca gactgtattt ctcttgcttc aattactctt
    12781 tctcgaattg ttttactatc ctctcctttt ttataagatg acaattcgtt gatatttaca
    12841 ggtgttactt caacatgcaa atctattcta tctaacaaag gaccagaaat tttattcaaa
    12901 tagcgttgta ccactccagg gccgcagaca cattcttttt caggatgatt aaaataacca
    12961 caaggacaag gattcatact agcaaccaac ataaaatttg caggataatc gactgaaaat
    13021 ctagctctag aaatatttac ttttcgttct tccattggct gtcgcattac ttctaaaacc
    13081 gttcttttaa attctggcaa ttcatctaaa aacaaaacac cattatgagc taaagaaatt
    13141 tctcctggtt gaggatgtcc acctccacct accaacgcaa catcagaaac ggtgtgatgt
    13201 ggtgaacgaa aaggtctttc atacaacaaa gccgaatttg cttttaatct tccagctaca
    13261 gaatgaattt ttgtagtttc taatgcttca ctcaatgata aaggcggcaa tattgttggt
    13321 aaacgtttag caagcattgt ttttccagca cctggaggtc caattaaaat tacattatgt
    13381 ccaccagccg cagcaatttc taaagctctt ttgatatttt cttgtccttt aacatcggca
    13441 aaatcaaact ctatatgttg gatattgcta aaaaattctt ctcttgttga aactactgtc
    13501 ggttcaatta ctttcttatc ttctaaaaaa tcaactacat catcaattgt ctcaacgcct
    13561 atcacttcca aaccatcaac aattgcggct tctctagcat tttgttttgg caaaatgatg
    13621 cctttatatt tttcttttct cgcttgaata gcaatgggca aagctccttt tattgcttga
    13681 actttgccat ctaaagaaag ttcacccata ataatataat cctctaattt ggaatgacca
    13741 atttgctccg aagctgctaa aatcccaata gcaattggca aatcataagc agaaccttct
    13801 tttctaatat ttgctggtga taaattgatt attatcttaa ttcttggcat cgtaaaacga
    13861 ttatgccgca atgcactctc aattcttgaa aggctttctt ttaccgcaga atctggtaat
    13921 cctactaaat aataatcttt tcctgaacat acattgactt caacagtgat ggtaattgca
    13981 tctactcctg aaacggcact cccaaaggtc ttcactaaca taataagaaa tttatactca
    14041 atctataaac gcaaaacttc ccaaattcca taaaataaga ccgttgcaag gtaatcatta
    14101 ttcataaatc tcaagccaaa ttccttcaat cttcttcgtt actcatttat ttttaatcct
    14161 ctttatcaat gtgataacta ctgtaaaaat aattgagtgc cttgaatatt tttgtaattt
    14221 gacttttgct acggtcttaa aaaacttgga taattcaata atataaaatt gaaataataa
    14281 aactatttga agacaaaaaa attagaaatc ctcactaaaa cccaaaacta tttttcaaat
    14341 agggtctaat tttttccaaa ggaattttca attcagttgg tccaaatgca taacttgaaa
    14401 cctcatattc attgtaataa aaagtaaggt tgtccttatc gattgtaaaa ttatcattaa
    14461 aataaaagcc tgccttttga ttttcatcta accaacctgc ttcaaatgct ccattttctt
    14521 gtaaactttc tttttgcgga atttcacgaa tctgcttaaa ataaaattct ccaatttcta
    14581 ataatgcttt atcatgctta tctttcagta cgtcttcaat tttaataagc gtatttgttt
    14641 tccgattata attatcaaaa ataacataat gacttccgtg agctccacca gtataagatt
    14701 ctaatgccat acgaaaactt ataatatttg agttattatt taaaatatta atctctgttt
    14761 gattatacca aggaaaatcc attggactct cttcaacata agatttatat tgttgcataa
    14821 aatcatctac aatactttgt atcgttgaat agccactttc attttcctcg cctattaaag
    14881 aaactctttt aataatagaa tcatttaatt gttttccaaa agcactatga atatcaaaaa
    14941 gtggataaat aaactcaatt gaagttgtgt cgtattctga agctcctttt actacggtgt
    15001 ctcttttgat tgatttcatt tcataggcta aagtatccgt gtatattgca tctttcgact
    15061 gagcatttgt attttgtcca ttatttttac atgcagtaaa aataaaaagc aaaccaatta
    15121 caattattaa atttttcttc ttcatttatt taaaaattaa tctattcgta tttaaaacta
    15181 tctttcaaat atggtctaat ttccgctaaa ggaattttta attctgttgt tccaaatgaa
    15241 taagatgaga tttcatattc attataataa aaaattagat aatttttgtc tatcaaaaag
    15301 ttatcattaa aataaaaccc accttttgga ttagttgctg accaacctgc attaaaaata
    15361 cccaagtccg ttagtttttt atcctgagca attttacgag tttccttaaa ataaaactca
    15421 ccaattttta aaagattttc atctctttta tttttaaaaa tatcatctat cgttaatctt
    15481 tttccacttt tcgtatcaaa attatcaaag actacagata tatcttcatg cattcctcct
    15541 aaatagattc taataaaaga ttttaaagac aataaacttg gtgtattatt taaaacttca
    15601 acattagaat agttatacca aggaatactt ataggactat ctcctaaaaa gagcttatac
    15661 tcatgcaata agctatcgac aaaatcctca atagtatgat aatttatatg aataatatta
    15721 ccttcttcat cttcttttcc taatattatt cgctctctaa cagtatcatt caaattcttg
    15781 cctaaagccg atgaactatc aaaaaatgga tatgctattt ttattattgt tgtgtcctta
    15841 tctttatgaa attgcattgg atatatccta tctacaattt tcattttgta ttgtaaagta
    15901 tctaaagaaa cactatttct acgctgatta ttgcgaaaag cattatttgt acaagctata
    15961 accaaagtca atattagcag actaaataaa tatcgaaaga agttcataca gttttatagt
    16021 ataacaaata taaatactaa ttcaattctc aactaaaatt aaatcaaaag ctttaattat
    16081 tttgtaatat ttaacttatc taccattgat aatatcttat tggtttgttc ttccattgta
    16141 atatgagaag tatcaataac aatcgcacct tctacttgaa ccaatgggct atcagctctt
    16201 gttgaatcaa tatggtctct gtgcaataag tttgccttcg ttatttcaaa tatttctggt
    16261 ctatcttcca aattcaactc tttcattctt cgtttggttc gaatatcaat atctgcaatc
    16321 ataaaaaatt tcaattctgc atcaggcatc actacagatg caatatctct accgtccata
    16381 acgacacctt ttttctgtgc cattagctgt tgttgttgaa ccattttacg acgtactgct
    16441 gtaatcgtac tcacctcaga aaccagttga ttaatttcca tttgtcttat tgcttgcgaa
    16501 acatcgacac cattcaaata aataagcaat tgctctcgtt ctcttttaaa ttcaatattt
    16561 atattggaca atgcattttt tacagcttgc tcatctttta aatctatttt attattaatg
    16621 aaataaaacg taacagctct atacattgca cctgtatcaa cataaacgta atttaatcgt
    16681 tgagcaatta tctttgctgt tgaagatttt ccacatgcag aatatccatc aattgctata
    16741 ttaatttgtt ccatttatta ttttgctttt tttccaaact catcaatctt aattcttaat
    16801 gatagaaaat gatcgcttcc cgcagcatgg taggcaccaa atccataatc tatactaaat
    16861 tgtctaatat ttaaacctaa accagccgta attccagcca ttcctttttt ttgcgtactt
    16921 gctaattcaa ttcttcgcaa atgattataa cccactctca aacgtacagg tttccctgca
    16981 tctatttctg tggcaataat aaaatggcct aaaatgtcat tggcaaattc ttttctttta
    17041 ttgactaccg ttttgccaaa agcattggtc gtagcagagc cagaaactgt taatttaaac
    17101 ttctgtaaat catgtgctac aataatcaat gcaataggga ttttagtaaa tttcttataa
    17161 aatgaaacat ctacttgcag tggcaacttt tcacgttcac tatttttatc atatctttta
    17221 agttgaaatc cagcatttct caacaacaca ctaaatgata tttttttatc tttattgtga
    17281 taacctaaag caatatcagt agccaaagcc aaagaattgt attgtgccaa atttgaataa
    17341 atcaacttta aattcatacc ataatacaaa tgttgccaat aatgagccgt tccaacctga
    17401 aaattaaaat cagcagcacg aaattctccc aacaaatttc ccgcattatc tcgactttca
    17461 aatttcccat aagaagcata ttgagccgta taggttaagg tagattttat acgtttatgg
    17521 tattgagcat aggtcaaatt tccaatatta atatcaccaa taaaaaaact attagaaaag
    17581 gcaatctgct tattcaatgc agagtctatc aaactagggt ttgaaattgc tgaagttaaa
    17641 tcattatcga tagtacttaa tacaccacca cccaaagctt catttcttgc agaaatagga
    17701 aatttcaaaa actgaaatac agacttacca ccaatctgtc caaaaatagt agaaacagaa
    17761 aacaaacaga gtaagaaaat gtattgctta atcataaact aaaaacagtc cctaagataa
    17821 ataaaaaatc agaaatcggt aatatttttg aggaatctcc aattactcta agaaattagc
    17881 tattaaataa aaaagaattt atatatcaaa tttaaaattg tcatgtctta aattaaataa
    17941 ttctaaaatc caagatttta ttctttttat taaaaaaaat atttaccttt gcatacttaa
    18001 aaaataataa taactgtatt atgagtttaa catcagaaaa gaaaattgag atttttaaag
    18061 agtttggggg ttccgaaaaa aacacaggat cggttgagtc tcaagtagct ttaattacac
    18121 acagaattaa ccatatttct gctcacttac gtgaaaacag aaaagaccac tcttcaacac
    18181 gttctctata caaattggta ggacaaagaa aaaaattatt aaaatatctt gctaaccaag
    18241 acattacaaa atatagagct ttaattgaaa aattaaatct cagaaaataa gtaacattca
    18301 attttttaag cgaggttttg acctcgcttt tttctttata tacattcaaa atattcctat
    18361 gattcaattt aatattcaaa agacaaaaac catatataat aacggtcaag aagttactat
    18421 cgaaacagga aaactagcta gacaagctga tggtgccgtt gtattaactg ttggaaaatg
    18481 tatgatatta gcaactgttt gtgcctctga tgcaccaaaa gaaggcgtag atttcttccc
    18541 tttgacagtt gattatcaag aaaaattttc tgctgcaggt agaattcctg gaagtttcca
    18601 cagaagagaa gctaaattat ctgactatga agttttaatt tcaagattgg tggacagagc
    18661 tatccgtcca atgtttgcag ataattttgt aaatgatact caagttttaa tttctttaat
    18721 atcaagtgat gaaacaattg cacctgatac attagtaggc ttagcagcat ctgcagcact
    18781 ttcagtttct tcaattcctt ttgaaggacc aatttctgaa gtgagagtga ttaaatcaaa
    18841 aggcgaattt attgtaaatc ctttggtagc ccaaatggaa gatgcagagt taaacatcat
    18901 tttggcagga acattagaca atatcttaat ggttgaaggc gaggctgctg aagcttctga
    18961 agctgattta attgaagcaa ttaaaactgg acatgctgtt attaaacaac attgtgaagc
    19021 tcaattagaa ttacaaaaag ctgtaaacaa agcaaaaaga gaggttcctg aaccagaatc
    19081 tgatgaggac ttatatcaat cagtaaaaac atttgctgaa gataaaataa aagaaattgc
    19141 aaaatctagc actggaaaag cagaaagaaa agcggcattt aaagctatta aagaagctta
    19201 tttagaaaca atttctgagg aagctgctga aactttcaac aaaggtttat tttcaaaata
    19261 ctatgcaaaa gttgagaaaa atgctatcag acataccgtt ttagatgata aaatcagatt
    19321 agacggtaga aaaacagatg aaatcagacc tttatctatc gaagtagatg tactaccaac
    19381 accacacggc tctgctttat ttacacgtgg agaaactcaa tctttatcta ctgttacact
    19441 aggctcaaaa caagatgaac aaatgttaga caaagctgaa agtttaactt atgctaaatt
    19501 tttattacac tataacttcc ctcctttcag tactggcgaa gtaaaaccta tgcgtggtac
    19561 atcaagacgt gaagttgggc atggtaactt agccttacgc tccttaaaac aaatgatgcc
    19621 agctgatgat actaatccat acactgttcg tatcgtttct gatattttag aaagcaacgg
    19681 ttcatcatct atggcgactg tttgtgctgg ttcattagca ttaatggatg cgggtattcc
    19741 atttaaaaaa ttagtatctg gtattgcaat gggtatgatt gctgaagacg gaaaatatgc
    19801 tatcctttct gatattttag gagatgaaga ccacttaggc gatatggact ttaaagttac
    19861 aggtactaaa gacggtattt gtggtgtaca aatggactta aaagtagatg gtttatctta
    19921 tgaagtttta gctgaagctt tagaacaagc tagaaaaggc agattacata tcttaaatgc
    19981 aatggaacaa gtgattgctg aaccacgtcc agaattaaaa cctcacgttc ctagagctca
    20041 cgttatggaa gttccaaaag aatttattgg tgcaattatt ggtcctggtg gaaaaataat
    20101 tcaagaaatt caagagaaaa caaatacgac aattaccatc gaagagttta aagaaaaagg
    20161 catcggacaa gttattattt tatctaacaa taaagataat atgaatgctg ctatcaacag
    20221 aatcaaaggc attgtggcag ttcctgaagt tggagaggtt tatgaaggaa aagtaaaatc
    20281 tattttggca tttggtgctt ttgtagaatt tatgcctgga aaagatggtt tattacacat
    20341 ttctgaagtt tctcataaac gtttagaaaa cttagatggc attataaaag aaggtgatat
    20401 tgtacaggtg aaattgatag gaattgaccc aaaaactggt aagttcaaat tatcaagaaa
    20461 agtattaata aataagaatg aagaagcata attcctaaaa tcctataaat cctgattcag
    20521 acaattacgg agaatcaaga ttattagagc caagaatcaa gactttattg agatagaata
    20581 attaagaaaa aggaatttag acaaaaggcg ataacacctt ttgtttgcga gaaaagaggc
    20641 tgttcgtaaa tttttgtgta gcctcttttt tataatttat ccgaatttag gtcacaatat
    20701 ttcttcccaa ttaaatgtat tctaattgtg tgaaacgatt ttaggataag aaatatcttg
    20761 aagtaaaata caatctcgtt cttaactgaa ttagtgtaaa cttttaataa ctgcttcaca
    20821 aatctcatgt ggtataaact tactgacatc tcctttccca attataattt cccttacaat
    20881 ggtagatgaa atcgctgata attctggaga ggataatatt aaaaaagttt ccacttcagc
    20941 atgcatcatt tggttcaatt gagcaattgt tttttcatat tcaaaatctg cggacgagcg
    21001 gatgcctctg ataatataat tggcaccaat ttctttacaa aaatttactg ttaaaccttg
    21061 ataagtttct actcgaacat tagtttcatt agcaaatact ttttttatcc aatttgttct
    21121 ttcttctaaa tcaaacaaat actttttttg tgtatttaca ccaatagcaa ctacaatttc
    21181 atcaaaaact tttgcagctc tttttacaac atcaacatgg ccaattgtaa ttggatcaaa
    21241 agaacctgga aaaatagcaa tcttcttcat actaatctgc gtatttaaaa aaccaaaaat
    21301 gggtttgtcc gtactttctt aaattaaaaa aattaggatg ttctaaaaag ctgtgtcttg
    21361 gagaagtttc cataataaac caaccatttt cagtcaataa tttatattca aaaattaagt
    21421 caggaatttc atctatacat tctaatggat atggaggtcc tgcaaaaatt aaatcatatt
    21481 tataaggtgt ttgcttgata taacgaatcg catctgcctt aataatttga tgattgggga
    21541 cttgagccat tgcactcatc tcctgaataa acttgatact aatgggagat aaatctacag
    21601 aagtaatttg attacagcca cgtgaaaaca tttcaattcc aatatttcca gtacctgcaa
    21661 acaaatccaa gaaatttata gattcataat cgatatggtt ggtcaaaata ttaaacaaag
    21721 cttcttttgc aatatccgtc gtcggtcgag ctggaatatt ctttggtgga gaaaaatgtt
    21781 ttcccttcaa tttccctgca ttaattctca tattttccta agcacaataa gttataataa
    21841 tagtgtaatg gatcattggt ataataaaac gagtaaccta aatcgtgatg caaaattcta
    21901 tctatctcaa aatttcgaat atagctataa ctaaaagtgt acattggtga tttctcttca
    21961 ataaaaccat acaaataggt tgcaaattgg ttagtatcta agcctaaagt ttcataagct
    22021 aatttcagaa aatacaacaa atcttgttta tcctgcacat aaaacttatt aaaataacta
    22081 accttattct tgtctttcaa tactattgtc aaatcatttc cagcaatatg agctaataaa
    22141 tgactttgga aagaaagttt tgaattggta tatttcaaaa gagctaagtc gccaaaatgc
    22201 caaacaacat ttacaaatcg atgaataaaa aaattatata catcaatagg tagaacataa
    22261 tacaggtatt gttcctctga aattttatct gataaaatct tatccattgg aagtgtatgc
    22321 attcctaaaa acgacatcat ttgatttggg ttttcagtct gctcaggcaa taacataaac
    22381 tgatttgtag aaaatgtaat tcttacttta acgaatgaca atttgaaata ctcattttcg
    22441 ttagaaaata ttttgggcaa tacttcatca taacgaatgt tttcaaaatt cttattaata
    22501 ataacagaat caatatccaa aacttttgaa ttagtcgtat caaatagtaa ataagttatt
    22561 gcataaggtt gcagatgaat ccacaattct aattcaatat ccttttgtat taattcgact
    22621 gctgttctaa taacaatgat ttaagaccac taagttataa tatttttttt atttatctat
    22681 gataaaataa ctatacctac gcatacttta aataaaaaat cattaaaaaa atttgggatt
    22741 tatactaaag tatcctacat ttggcttatg aatttacatg aatatcaagg aaaagaatta
    22801 ttaaaatctt ttggtgtaaa aatacaagaa ggcatagtag ctgaaacctt agaacaagcc
    22861 aaccaagcgt accaaacaat cgcaacaaga acaggaaaag atggcgtagt cataaaggct
    22921 caagttcacg ctggcggacg tggtaaaggc ggtggtgtta agtttacacc taactttgat
    22981 tcttttaaag aaaaagcagc tcagatttta ggtatgactt tagtaacacc acaaactgga
    23041 gctcaaggaa aagtagtaca caaaattcta attgcagaag atatgtatta taaaggcgaa
    23101 acagagccta aagagtttta tctgtctgtt ttattagata ggaataaaga atgcaatgcc
    23161 attatctatt ctcaagatgg tggtatggat attgaagaag ttgcagaaaa aacacctgaa
    23221 agaattttta aagaatggat taatccaatg gtgggtattc aaccttttca agctcgaaga
    23281 attgctttta atttaggatt agaaggacaa gcatttaagg aaatggtcaa ttttgctatg
    23341 aatgtttaca aagcttacga aaaatctgat gcctccttat ttgaaatcaa tccagtttta
    23401 aaaacatcgg acaataaaat tgtggcagta gattgtaaag taaatttaga tgacaatgcc
    23461 ttatatcgtc aaaaagattt agaagcacaa agagatttat tagaagaaga tccaacagaa
    23521 gttgaagcac aagaagccga tttaaactat gtgaaattgg acggaaatgt aggttgtatg
    23581 gtaaacggag ctggtttggc aatggctact atggatatta taaaattaag tggtggcgaa
    23641 cctgcaaact tcttagatgt tggaggtaca gcaaacgctg aaagagtaga aaaagctttc
    23701 agaattatta tgaaagatga aaatgtaaaa gctattctaa ttaatatctt tggtggtatt
    23761 gttcgttgtg atagagttgc ccaaggcgta attgatgcat ataaaaatat tgaaaacatt
    23821 aatattccaa ttatcgttag attacaaggt acaaatgcgg atattgctaa gaaaatgatt
    23881 gatgagtcag gcttaaaagt acaatctgca attttactat ctgaagctgc tgaattagta
    23941 aataaagcag tagcaaatta aaataaacac taaataagtt ttgaaaacta aaaaaaaaat
    24001 ctacatttgc aatatcaata tgagaacagt aaatttttcg atgtgttgtt gttgtttcat
    24061 ttcaaaaaat gggaggcaat aatttattgc aattaaaata aaaataaaag tcttccacca
    24121 aaggaaggct ttttttatat aaaaagatta ttaaacaatc aaacaattaa ttatgagtac
    24181 tcaaaaattt gaaaccttac aagtacatgc tggtcaagaa ccagatccaa ctactggaag
    24241 tagagcagtg cctatttatc aaacaacttc ttttaatttt aaaagcacag aacatgctgc
    24301 taatttattt ggattaaaag aatttggaaa tatctataca agaataatga acccaacgac
    24361 tgatgtcttg gaaaaaagaa ttgctgcatt agaaggcggt gttgctgggt tagcaacttc
    24421 tgctggtatg tctgcacaat tccttgctct aactaatatc ttagaagctg gtgaaaactt
    24481 tgtaacgaca tcatttttat atggtggaac atacagccaa ttcaacgttt ctttcagaag
    24541 attaggcata gatgcaagat ttgctgatgg cgataatgtt gattcgtttg agaaattaat
    24601 cgatgacaaa acaaaggcaa tttatttaga atcttacgat aatccaagag gtaatgtacc
    24661 tgattgggat aaatttgctg cattagcaaa aaaatatgat attccattag tcgtagataa
    24721 tacgtttgga atttgcggtt atatgttccg accatttgac catggagcaa atgttgttgt
    24781 agctgctact actaaatgga taggtggaca tggaaatagt attggtggaa ttatagttga
    24841 tggaggaaat tacaattggg gcaatggtaa attccctcgt ttcagtgaac catcagacag
    24901 ttacaatggt ttaaatttct ggaatgtatt tggaactcct ggaccatttg gaaatatcgc
    24961 atttgcaatc aaagcacgtg ttgaaggttt aagagattat ggaaatgcaa tcagtccttt
    25021 caactcattc ttattacttc aaggattaga aacgctttct ttaagagctg aaagacataa
    25081 tgaaaataca attgctttag ctaattggct aaacgaacat ccaaaaattg aaaaagtaaa
    25141 ctttactggt ttgcctgata gtccatatta cgaattagct aaaaaatact tacgtaaagg
    25201 acattttgga tatgtattca attttgaaat caaaggtgga tatgaagctg caaaaacttt
    25261 tgtagataac ttaaaaatga ttagcaattt agcaaatgtt ggagatgcta aaaccttagt
    25321 tatccatgct gcatcaacta cacacgagca attaagtcca gaacaacaat tatcggcagg
    25381 tgttcttcca gcatccgtaa gaatatcagt aggtattgag catattgatg atatcaaagc
    25441 agatatcgaa caagcattag cattagtata aatttataat caatcaaaaa aagacaatat
    25501 cattggaacc taatatttta aaatataaag caccatttcc tttagaatcc ggcactatcc
    25561 tacctgaaat tgatattgct tacaatacat ttggcacttt aaatgctgac aaatccaacg
    25621 tagtttggat ttgtcatgca ttttctgcca attccaatcc aacagaatgg tggcctggaa
    25681 tggtcggcga tggtttattc ttcgacccca aagattattt tattgtttgt gccaatatgt
    25741 tgggttcttg ctatggctca actggtccat tatctatcaa tccagaaact ggcaaaaaat
    25801 attataaaga tttccctgtt gttacagttc gtgatatggt caaatcttta accatactta
    25861 gaaaacattt aggtatcgaa aaaatttatt tttcgtgcgg attttcaatg ggtgggcaac
    25921 aattattaga atggataatt acggaaccca ctatttttaa aaatatttta ttaggagcga
    25981 ctaatatcaa acattctccg tggggcattg catttaatga atcacaaaga atggctattg
    26041 aagccgatgg aacttttggt caagaagatg acaatgctgg aattaatggc atgaaagcag
    26101 caagaagtat tggcttgatt tcttatcgga attacaaagc atacaatatt acacaacacg
    26161 atgaggataa ttctaaacta gaaaatttca aagcttgtgg ttatcaacgt tatcaaggcg
    26221 agaaattagc taagcgattt aatgcttatt cttattatat tttatccaaa gcaatggata
    26281 atcacaacat agaaagaggt cgtggtgcag caaaagaggt attagaaaat ataaaaagta
    26341 atatcattgt attgggcata aaaagcgatt ttttatatcc attggaagaa caagaattaa
    26401 tggcttcctt aatgccacat tcaacttttg tagcgataga ttctcatttt ggccatgatg
    26461 gcttcttaat tgaaaatgaa caaatctcta aaatattaag cactttttta aaggcttaat
    26521 tattgaagtt tatttgaaat attagaatgt tttttattaa ttgattatac tagataatcc
    26581 ttatttttgt tgaaattaca agatgctgaa aattgcagta gtcatattaa attacaatgg
    26641 caaacattgg ttagaaaaat ttcttcctaa tgttattgaa aataccactt attctaattc
    26701 agaaattatt gttgctgaca atgcctccac tgatgactca attatatttc taaaaacaaa
    26761 ttttcctact gtaaaaatta ttcaaaacaa agaaaacaca ggatatgctg gtggttataa
    26821 cgaggcttta aaaagcgtaa atgccgatgt ttattgcttg ctaaattcag atgttgaagt
    26881 atccaaaaac tggttggaat atatactacc gacttttgaa gatgaaaaag ttatggctac
    26941 acagccaaaa atcctatctt atcacaataa atcagcattt gaatatgcag gtgccgcagg
    27001 tggttttatt gataaatatg gttatccttt ttgtcgtggt cgaatttttg ataaggtaga
    27061 gcaagataca ggacaataca atgaagtcct agaaattttt tgggcttccg gtgcttgttt
    27121 gtttgttcgt tcttctattt ttcatcaatt aggtggcttt gatgaagact tctttgccca
    27181 tcaagaagaa attgatttgt gttggagaat taagaactta ggctataaaa tcttatacgc
    27241 tccaaaaagt gttgtttacc atgtaggtgg tgggacttta aacaaatcta atcccaaaaa
    27301 gacattctta aatttcagaa ataatcgttt tttattactc aaaaatctaa ctcaaaaaga
    27361 atatcaaaag atttcattta ttagaaattg tttagatatt attgctgcat tcgttgccat
    27421 attgaaaggg aattttcaag aagcgaaggc aattatccat gctttaaaag attttcaaaa
    27481 acagcagtct ttgtatttaa aaaagagaac aaacaacgaa cttttaatcc gtctaagtgc
    27541 agtagatgtt ccaaacaaaa aaggcattta ttccaaaagt atcctctatc aatattttgt
    27601 aaagggaaag aaaaaattta gcgaaatatt tcactaaaac ttcaaccaat gccaacgcac
    27661 tttgtgtgaa gatgctgtat tgaattccaa tgctggaaat ggtaatttta taatattgat
    27721 aatgcctaaa caagtttttg cccatttacg ttttacaggt atttttgtcc aatcaatatc
    27781 catagatata taatatcttc tgtaacgttc tatatcactt ctatcaataa tatcagcagg
    27841 gtctgtacca tctaaaatat tatcgccact caaatcattt ttagaccatc ggttttcata
    27901 accacccaac attccaccag ctccaaagcc aaatgatact tgtaatgctc tagcaaaatt
    27961 ttgttttttt tctttaataa atgaaccagg agtaacacta aaccaaatag tcatggcatt
    28021 ataatctttc agcatcgttt ctgcaaaaga agttccaaaa agtttatcag cacgttgttt
    28081 gatttcagtg tctttatatc ttttagtata attaatcgga aaagaagaca ttttcatcat
    28141 tactctttgt tcgtgccacc aatattgctg aataccaaac aataaacttc ctgtcaaatt
    28201 catacccata tcaccccaag aaaatcccca ttttttttga aagccatcat gtacttcaat
    28261 agacaattgc caaaggtttc ctgtaagcat tccaatccaa attgcatgtt tttctttaac
    28321 acccgaccat ttgaataaat cataagtcca tttactttca aaatagcaac tcatgccatg
    28381 tcccaactta tcaatattat taaaatcacc ccaatcgttg aaagtatgaa actttgattt
    28441 ttcagtttta gaataccaag ctgtactcca ccaagtattg gctgcggcat ataaaccacc
    28501 ttgcaaacca gccaccaatg caactctcct tttatttaac tcaggagaat tttcaaaaaa
    28561 tttatagggc tttgctaatt ttaattttcc aacataaggc tcttcaatag gattcgtatc
    28621 tacaaaattt aaaacagaaa tgaccgtatc ataagcattt aaaccatctt gatgtacttc
    28681 aaatattaaa tcatcttgtg caaataaaaa attataaggc acaaatagtg aaaataatat
    28741 cgcaaatatt ttgaataaat aaatacgata tataatccaa tttttattca cttaatagaa
    28801 ctttttttac ggactctaat acatcaatta catttgctct attgttactt tgtgcataca
    28861 ctctcaacac tggttctgta ccagatgcac gtatcatagt ccaatcttca tcattcaaaa
    28921 aatacttcca accatctatc gtttctcgac cgactatatt atattttcca aagtttttat
    28981 agttatcttc agtacaattt ttaattacgt tttgtttgat ttcttcagag atatgcaaat
    29041 catatctatc atattcaaat gagccaacaa tttcatacac ttcctgaatt aattcattga
    29101 ttgttttgcc agtctttgcc ataaattcca tcaaaactaa agcaatccat acaccatctc
    29161 tttctggaat gtgtccctta atcgccaatc ctccactctc ctctccacct actaaaacat
    29221 catcattgac cataatttca caaatatatt taaatccaac tggagttacg atacattcta
    29281 aaccataagc ttcacataat tttttcactt tattagaaac agtaaatgaa acaacaactt
    29341 tgcctttctc tcctttgtat ttatacaaat aatgtatcaa caataataaa atatgatgtg
    29401 aatcaacaaa attaccttcg ccatcgtaac aaccaattct atcagcatca ccatcattag
    29461 caaagccaaa atgcaaatca gaagagtttt tcattaattc tgagaattct aataaatttt
    29521 tatgaattgg ctctggagct tgaccgtcaa aacctgggtt atgctcacag tgtaaataca
    29581 ctaaactgtt tggcagtaat cgtcttgcaa tattttgacc agcaccatac attgcatcat
    29641 aaccaaatac cattccagaa ttacggattg cttccatgtc aaaatttttc tcaacatgat
    29701 tgaaataatc agtttccata tccacatatt ccaacaaacc ttgtttttga aaatcatcta
    29761 agtttgtttt aggaatttca atactttctg gaattaaact ttccacaata gcaatatctg
    29821 cagggatagt tggtccacca taattagact ttaacttaaa cccattataa ctcggtggat
    29881 tgtgcgatgc ggttatcaca attcctgctg ttgttttata atttacacaa gctaatgaca
    29941 acattggtgt tgcacaaata cctttgtgag caaaaacttt tacgccttct tggcacaata
    30001 ttttaatcgc tgtttccaca aaaagaggtc cagcaaatcg acaatcatgt cctattgcaa
    30061 cacttgcatt ttcatcaata gattttaccc attttgctgt tgcataagca actctagcta
    30121 cattatctgt cgtaaattct tgggctatta tcgctctcca accgtcagtt ccaaatttta
    30181 tgttattatt cttcataagc ttcaaatata gcattttaaa tttttattac ataaaaattt
    30241 tgaatgcata ttttacaaac aaaaaacata cttttgcatt gtctctaagg ggtgctggtg
    30301 gaaacactgg ctgagattat acccattgac ctgatcaagg taatgcttgc gaagggaagt
    30361 gaccaaattt atttattatt aaccaagaga attggcccct tgttcttgtt tatttttttg
    30421 aaatgaaaac aagaatagtt ttaagttccg ctctcgtatg tttagcaaac atactctttt
    30481 cccaacaaat ttttaaagga aaaattgaaa atgaagcgaa gcaaccgctt acaaatgttg
    30541 ctatttttaa cacacaagac tcatctgtta tttactccga tgagaaaggt aattttgaaa
    30601 tagaaattac aaaaaaaaca cccattagct tatcattaga tgcttataca agtatttatg
    30661 atagtattta tcctgagcaa caggaaaaaa cttttacact ttattataat ttacaacaat
    30721 tagaagaagt gcaagtaatg gcgactagag ctgggaacaa gacacccaca agttatacca
    30781 acttaagtac tgaaaaaatt caacaacaaa attttgggca agatttaccc tatttattga
    30841 atggtacttt atcaacagta gtttcttctg atgcgggtgc aggtgttggc tataccaaca
    30901 tgcgaatacg tggtgttgac caaactagaa ccaatgttac catcaatgga attcctatca
    30961 atgatgctga atcacaagtt gttttctggg taaatatgcc agatttagct tcctctaccg
    31021 aaaacataca aatacaacgt ggtgtgggta cttcatcaaa tggtgctgct gcttttggag
    31081 ctagtgtcaa tatcagttct aataaaataa aacaaaaacc ttatttagaa ctcgataatt
    31141 cttttggctc atttaataca ttaaaaaata ctataaaaat tggaacagga atattcaaaa
    31201 agcattttgc tattgatgtt agactttcta atatcatttc aaaagggtat attgacagaa
    31261 gcagtagtaa tttaaaatcg tactatgttt caggtatttg gttgaataat aaatctattt
    31321 taaaatttat tacttttggt ggtcgtgaaa ttacttatca agcgtggaat ggtgtacctg
    31381 aaagtcgtat caaaaatgac caagaaggta tgttggctta tgctgaacgc aatggacttt
    31441 cagaaactga aaacgataat ttattgaata gtggtagaac ttacaactat tttacgtaca
    31501 aaaatcaagt tgatcattat aaacaaacac actatcaatt acattttaat catagcttta
    31561 gcaatgtttt aaagctcaac gtttcattgc attatactag agggaaaggt tattatgaag
    31621 aatataaaaa ccaacaagat ttagaagatt atggcattga accaatctcc ataaaagata
    31681 cttccattct aaatactgat ttaattcgca gaaaatggtt agacaatcat ttttatggaa
    31741 tggtatttaa tttgaattat actaagaaca acttagattt ggtttatggt ggtggtttta
    31801 atcaatattt aggcaaacat tttggagatg taatttgggc tagatatact tccaattctg
    31861 aaataaatca taaatattac gataatgatg cttcaaaaat tgactttaat aactacctca
    31921 aattaaatta tcaattcaaa aagaatacac tatttgctga tgtccaatta cgtgtagttc
    31981 attatgcgtt taatggtttt gcagatttaa gtggtaacac aaatgagaat atgcaaaaag
    32041 ttaattatgt atttttcaat ccaaaagctg gttggatgta tcattggaat aatcaccaca
    32101 atacatttat ttctattgct attgcaaacc gagaacctat tcgtgatgat tttattgata
    32161 atggacaagg caaacaacca agacacgaaa ccttatacaa cttagaattt ggtcataaat
    32221 ttcagaaaaa taaatgggca ctaaatgcca actattttat gatgtattat aaaaaccaat
    32281 taatattaac aggaaaaatc aatgatgttg gaggttatac tcgtactaat gccaatcaat
    32341 cttataggac aggatttgaa atagaaacaa gttttcaagt ttttaaatgg gctacgattt
    32401 tagcaggagg cacttatagt ttcaataaaa tcattgactt tactgagtat gttgatgatt
    32461 atgataatgg tgggcaagtt gctatacagt ataaaaatac agatttagct ttatcaccaa
    32521 atcttatagc atcttggggt ttgaatttac aaccagtgaa agattttaat atttacataa
    32581 atgggaaata tgtgggtcgt caatttttag ataatgcatc agacaaagca cggtcaataa
    32641 atggatacta tacgatggat ttatccttag cttatcaatt aaatttaaag aaaataaaag
    32701 gtatagaatt tagtgtacaa atgaataata ttttaaataa aaaatatgaa aacaatggtt
    32761 atacctatag ttatatttat ggaggcgaac gtgtaaaaga aaacttttac tttccacaag
    32821 ctggtattca tgcaatgggt agaatttcta taaaacttta gtcaattgaa aattcaaaat
    32881 gaaatagggt tgcacttggc aaccctattt catttaaaca ataactaact aagtatttct
    32941 attcagccgt aattgtattc attattctcg tttgatttct gatactttct tggtgtatcg
    33001 cctcaaacat ttcttttaca aactcttcag tcaattcttt tttagcacct tttttcactc
    33061 tgtcattaac gatttcatcc caacgacctg tttgcaaaat tgtaatgcca ttatcttttt
    33121 tgaattgtcc aatcgctcta gcgatatcca ttctctcact cattaattct aagaaatagt
    33181 tgtctattct atcaatatta gcacgaagag cttctagttt atcttcttca taatcatcta
    33241 atttactatg acgaactact aaattagcca ccaattcacc tagttgttct ggtgtaattt
    33301 gttgtgctgc atcactccaa gcattgtcag ggtctctatg ggattctaac ataatgccat
    33361 caaaatctaa atctaaagct ttctggctta cataagctaa taattctcta tttccacaaa
    33421 tatgtgatgg atcaacgata atgccaattt caggtactaa tctttttaat tctacaggca
    33481 attcccacat tggtttattt ctgtattttg ttttttcata agttgaaaaa cctctatgaa
    33541 tagcaccaac tcttgtaatg cctactttat ttaaacgttc catagctccc atccataatt
    33601 gtaaatctgg attaatagga tttttaacta aaacaggaat atctacacct tttaaagcat
    33661 ctgctacttc ttgtactgaa aacggattta cggtagttct agctcctaac cataaaatat
    33721 ctatacctgc atttaaacaa tcttcaacgt gttgtgctgt tgcaacttca acagttgttg
    33781 gtttgccaat ttctttagcc gcattcacta accaagataa tccttctttt ccaatgcctt
    33841 caaacattcc aggacgtgta cgtggtttcc aaattccagc tcttaataca tcgatatttt
    33901 gattacctaa tcttcttgct gtttccatca tttggtcttc tgtttcgcat gaacaagggc
    33961 cagaaattac gattggtttc ttaaagttaa aaccccactc tttaattggt tttatttcta
    34021 aattactcat attgcaaatt taatttattg tttgttaatt acttattatt ttttttccta
    34081 ttgttttgaa atttctatgg ataaacaact ctatcataat gtaatacagt tggtaactca
    34141 tcataaaaat cttgtaataa tgctctccat tctttaaatc cttctgaatt tctaaaatct
    34201 atcatgtgat tttcataagt ttcccaataa atcatcaata aatattggtt ttttatttcc
    34261 ttacaagcat gtaattcgtg cttaatatat cctttggatt tattaataat ttcagatgct
    34321 ttcaagaaat tttcttcaaa ctttactgtt tgttcagcct tgatttttaa tgtaaatgct
    34381 tctagtatca tcttgttcta ttttaatatt tttttaattt gattagcgtc tttcataaat
    34441 tgatttaatt catcatattt tcctctgttg atataatttc tgaaactgta cattttctcg
    34501 atgtaagtat ctaaaacttc taaaatattc gttttatttt gaataaaaat tggagtccac
    34561 atttcaggag agcttttggc taaacggaca gtactctcaa atccaccacc tgccatttct
    34621 aaaatagcac tttcgtcttt ttctttttcc aataccgttg cagccaaaac aaaagaacta
    34681 atatgtgaaa tatgagaaac ataagctgca tggatatcgt gttgatgagc gtccatatat
    34741 ttcaaattca ttttaagtgc ttcatacatt ttttccacta attctacagc atcgctatcg
    34801 gatttttcct tgtcacaaat tatacaaact ttatgatcaa aaagattatt gatagcggca
    34861 gttggtcctg agttttctgt tccagccatt ggatgtgatg ccacatatct acttctattt
    34921 ttatgatttt gtacagcatc tacaattgca cccttcgtag aacccatatc cgttacaact
    34981 tgatggcttt ttatactatc caaaacaaat aacaattcat caatcaatgc attgacagga
    35041 actgtaagta ctattaaatc agcaatttca atagcttcat ctctaggtaa aattctatct
    35101 actaacttta aatctaagac tttttgattg gtagcatctg ttcttcccac tccaattatt
    35161 tcagtagcaa aattcctaat ttttaagtct tttgcaacag aaccaccaat taaacccatt
    35221 ccaaatacaa caactttcat ttcttaattt tctttataaa attcaaatat cttcaattct
    35281 aaatattgaa taatttttca attctcttca gtgcttcttg caaatcattt tcagtgctac
    35341 acaaagaaat tctgatatac cttgcaccat tcgtaccaaa aataaatcca ggtgtaataa
    35401 atacatctgc ctcatgtaaa attctttcgg tcaagtcttc tacattatta tatttatcct
    35461 caatttttgc ccatagaaac atacctactt gttctttggt gtaactacaa tttaaagtat
    35521 ctaaaatttg ataagcaata gttcttcttt ttttatacac ttcatttctt gtattatgcc
    35581 attcttggtt attcgtcaaa gcagcaattg ctccagcctg aactggcaaa tacataccac
    35641 tatccacatt agattttact tttaaaattg tatctatata agctttatcg ccaactatca
    35701 ttccaattct tgcaccagct agattgtgtg atttactcaa agaatttaat tcaatacaaa
    35761 cttcctttgc accatctgca tgaaaaatac tcattggtct ttcattattc aaaaccaaac
    35821 tgtatggatt atcattacag actaatattt tatacttttt tgcaaaagca attaattttt
    35881 caaaataagc aatattgcct tccgaaccag taggcatatt gggataattc acccacatta
    35941 atttggcttc attcaatatt ttttcatcaa ttgaatctag tttaggcaac caattatttt
    36001 gctcatctaa atcataaaaa ataggctcgg cacctgctaa ataagttgca gaagtataag
    36061 ttggatagcc aggatttgga attagcactt tatctcctgg atttaaaaat gcattagata
    36121 taaatgaaat tccttcttta gaacccaaca atggcagtac ctctgaattt gaattaaaag
    36181 ttacattata agttgcttga taaaatgatg ccaatccttg tcttaactct ggtgtgccaa
    36241 tataactttg gtatccgtgc gtattgggtt gttttgctgt ttccaccaat gcattaattg
    36301 tagcctcaga tggtggcata tctggattac caatacctaa atttataatt gattttcctt
    36361 gtgctctcaa ttgagctatt tctttcaatt ttgctgaaaa ataatactca ttaatatgag
    36421 ctaatttatt tgcaggttct ataatcataa aaatctttta tctactttta ttctgaaaca
    36481 ataattttct ttcctctttc atattcaccc aaaatcttca attctttaca taatggctca
    36541 atctgttttt tcatggtttg ataggcattt ttatcttcaa attctaaatc agcatacata
    36601 taatattgcc attcttcacc gatgactggt agtgattgga ttttagttag attgatttga
    36661 tgttcaccaa taacagtcaa tattttagcc aaacttccag gtgtatgaaa tgctctaaat
    36721 gaaatagatg ctttatttat aaattcactt ctactattga cttttgattt atcttccaga
    36781 attaaaaagc gagtaaaatt tcgtttatta tcgtgaatat cttctgccaa aatctctaaa
    36841 tgatttattt ttgcagctcg tttacttgca acagctccac gttttttgat ttgattttta
    36901 taaatttctt cagcacttaa tgctgtatct gaagattcta ctatttggat atttggataa
    36961 tttctgaaaa aattacgaca ctgcaataaa gcaattggat gcgaatgtac ttcttctaaa
    37021 tcttcaattt tttgaccttc taaagccatt aaattcattc caattcttaa ataaacttca
    37081 ccaatgattt ctaaattaga attaccgata ttggcatagt tatttaataa tgaaccagct
    37141 aatgaatttt caatagcaca gacagcaaaa tcggcttgtt gatttgccat aacatcaaaa
    37201 agcttagtaa aagaaccaca ttctacagtt tcaatttctt ctccgaagaa ctttatcgct
    37261 gcaatctcat gaaaagatgc tttaaaacct tgtattgcaa tttttgtttt cattttactc
    37321 taattttttc aaaaaaaaag tcccgaaaac tcgggactat aatttatcta ttgaatacag
    37381 cattacaatc ccttacttag aagtaaagta aaagtaataa ccatatacaa aattcataat
    37441 catagaacaa aacaaatcat ttttttcgtt ttttacaagc ataaggatat taaatattta
    37501 ttaagtggta taaaaatagt atagacaaag taaataattt gattatactt gctctcatta
    37561 tgttgaactt tgacactgta acgaacttag ctccttttga aaaacttgct tattgtaata
    37621 cgcaatatcc aacaatctta gtaagtggct taggttttca agaccaaaat aaagtaacca
    37681 actattgggg taaagtgcct gaatatttga aattacacgg tgctgatgtt tatttagcaa
    37741 aacaacatgc attcctaagt attcccgata atgccatacg tttaaagtat agaatattag
    37801 atattttaga agctacaaaa aaggataaaa taaatattat tggacattct aaaggtggtt
    37861 tggaagctcg atatgttgcg ggaaaattgg gtatccatgg acaaattgca agtattacaa
    37921 cacttggaac accacatcaa ggagcacact tggcagatat tgttttaggt aaaattccaa
    37981 ttcccaattt tgctatggca caacttacca atatttatgc taaaatatta ggcgatagtc
    38041 agcctgatag ttttcgagct gtattgcaag tttcaactca acaattaaaa aaattcaatg
    38101 ctgaagtacc tgatatagat agtatttatt gccaaagtta tgcaggtcat atcaataaag
    38161 attatcctgg aacaatgtgg cgaacattag ctggaattct taaaccctat gatggtaaaa
    38221 acgatggatt agttggtgtt gaatctgcaa aatggcaaaa ttacaaaggt ttgattgcaa
    38281 ctgaagatgc accaagtgta agccatggcg acatgatagg ttttgctcaa ggtattggag
    38341 agaaaaaatt caatgcacga gcttttattg cctatttatt acacgattta aaagaaagag
    38401 gctgttaaaa ttataagttt ttctaaaata ttttttgaat ttgaattgat aatatactta
    38461 agaaaacgta taaatattat agataaaatg cttccagaga aattaaattc aacagcatct
    38521 ttaaacattt ccataatcta attgtttgta ttaaaagact tatatgaaaa agaacatagg
    38581 aaaaacagat agaataatta gattttcagt tgcaatagtt attgcattgt tgtatttttt
    38641 aggtttgatt cctggtattg ctgcaaccat tttaggtgta ttagctgtta tttttataat
    38701 aacatcgttt attagttttt gtcctttata tcttccattt ggaattagca ctagacggaa
    38761 agacgcataa tataaaaaaa acggctggaa tataccagcc gttaaatttt agttcagcaa
    38821 tcttgaacgt ttacaaaatc tcaattgctt agcaaaattg attataagca ttcactaatt
    38881 tttgagctac tgcttgtgca gtataacctt caatatcatg tctttctaaa aagtgtacta
    38941 agttgccgtc tttaaacaat gccatacttg gagaactcgg tggatatggc attgtaaatt
    39001 ctcttgcttt tgcaacagct tcactatcta cacctgcaaa aactgtaact aatttggttg
    39061 ggtgcttctc gtttttagaa gatacaattg cagcagggcg tgcatttgcg gcagcacatc
    39121 cacatacaga attgataact aacaaagtag taccttcttt tgatgataat gcttcggtaa
    39181 cttcatctgc tgattttaac gcttggaagc cattttgtgt taattctgct tccattgggt
    39241 cggtcatttg ttttggatac atatttgttt taatttaata atgaaataat tgtatttgaa
    39301 aatatttaaa aataatagtt ctcacactat aaaaatccta attctaattt tgcttcctca
    39361 gtcatcatat cttgatccca aggtggttca aaggttactt ctacttttac attatttaca
    39421 ccgtcaattt cctttatttt atcttctact tctttgggta aactttcagc aactggacaa
    39481 ttgggtgatg ttaatgtcat tacaatatgc acattatttt ccatatctac ttcagcttcg
    39541 tacaccaaac ctaaatcaaa aatatttaca ggtatttctg ggtcataaat ttcgtgtaat
    39601 aattctatta catcgttttt tacttctata aatgttttat tcattttttc tactatcatt
    39661 ttagtttatt gcttggaatg cgacaccata agtttttatt ttttttatca tactcaccaa
    39721 tccattcgca cgtgtaggag acaaatgttc tttcaagcca attttatcta taaaatataa
    39781 atcactttca gctatttctt caggtgtgtg gccactcaaa acttttacca acaatccaac
    39841 tatacctttt gtaataatag catcactatc tgcaaaaaaa acaactttat ccgcttgttt
    39901 ttcagcatac aaccatactt tactctgaca accattaatt cttgtttctt ctgtcttata
    39961 tttttcatct aatattgata atgactttcc aaaatcaatt aaatattcat acttatccgt
    40021 ccaatcttca tagatagaaa aatcttcaat aatttcgtct tgaatatcgt ttatcgttgc
    40081 cataatctat taactcaaca tttttttagc tctcaaaatt ccttcaacta atacatctac
    40141 ttcttctttg gtattataca atgcaaaaga cgctctacaa gttccaggaa tattaaaata
    40201 ttccatcaca ggttgtgcac aatgatgccc tgttcttatt gcaattcctt gattgtctaa
    40261 taaaacacct acatcataag gatgaatgcc ttctaataaa aatgaaatta cagatgattt
    40321 atcttttgct gttccataaa tagtcaatcc attgatggca tttaatcttt ctgtcgcata
    40381 ttcaagcaaa ctatgttcat actccataat cttttgcaag cctaaatctt gtatatattt
    40441 aatggctaca ctcatagaaa tacatcccga aatatgtggt gttcctgctt caaacttaaa
    40501 aggcaatgca ttaaaagttg ttttggcaaa agatacattt tctatcatat cgcctccacc
    40561 ttcaaatggt ggcattgcat ttagaatatc ttcttttcca tataaaatac ctacacctgt
    40621 tggaccatac aatttatgcg cagaaaatgc caataaatca caatctaagg cttgtacatc
    40681 tatcggaata tgttgtatag cctgtgccgc atctatcata attttagcac ccttactatg
    40741 tgctaaagca attaattctt gaataggatt tatggtacct aaagcattag aaacatatac
    40801 aactgatact aatttagtct tagaagttat taaacgtgca gcatcttcca ataaaattgt
    40861 accatctttt tgtaatggaa taacttttaa aatacagcct ttttcttcgc aaagtatttg
    40921 ccacggaacg atattagaat gatgttccat ttctgtaacc acaatctcat ctcttgcttg
    40981 aataaattta cgtccaaaag tataagctac aagattaata gaattagttg tgcctttggt
    41041 aaaaataatt tcatggtcat gtttggcatt aataaattgc tgtacagttt ttcgtgatgc
    41101 ttcgtaaagg tcagttccaa tttgcgataa ataatgtaca cctctatgaa tattagcata
    41161 ttgatgttca taaaaatatt gctctgcttc aattaaaact tttggttttt gagtagatgc
    41221 tccattatct aaatatacca attgctttcc acgaattttt tcagaaagta taggaaaatc
    41281 agctctaatt ttttgaatat ctaacattac ttattttctt ttctaactat gaattccttt
    41341 ctcaaactat tcaacaaatc caattgttga gcttgggatt caaataataa ttttatttca
    41401 ttttctaaca acaagtcaat cttttgatgc aaacttctaa tttccaattc agattttaga
    41461 ttaataatat aatcattttc tgcacgttgt cggtctttct cttccattct attttgactc
    41521 atcattataa taggtgcttg aagtgcagcc aaacaggaca aaactaaatt cattaaaata
    41581 aaaggataag gatcaaatct atatttagta atagccaaaa cattgaataa tatccacaca
    41641 aaaagtataa aacaaaagga gataataaaa gtccaactcc caccaaattt tgcaatagaa
    41701 tcggtcaatt gttgtgctcg acttaattgt tcaacagact tatttttcag attcatcata
    41761 atcaaatctt cttcttcgat agatttcatg acaatatcat gtaaagattg aatatctttt
    41821 tcttgagatt ttaataattg ttgaatttgt tttgctctca tatttatata tttataccta
    41881 aggtagtaaa tattttatct tcaacaaatt gtttatttct atcagaaagt ttatctaata
    41941 cctcaccagc aaatgctaat gttagtaaac ttcttgacat ttcttttccg attcctcttg
    42001 cttgtaaata aaacaactca ctatcttcaa tttgtgaaga tgttgcacca tgtgaacatt
    42061 ttacatcatc agcaaaaatt tctaattgtg gtttagtgta aatattagca tcattactta
    42121 aaagtaaatt tctattgctt tgaaatgcat ttgttttttg tgctttctga gcaacaataa
    42181 ttttaccatt aaacacgcct gttgcaaggt cagccaaaat gcctttatac aattcattgc
    42241 tattggtatt gggttcattg tgtcgcacca aagttctatt atcacaatgt gcttgtccgt
    42301 gcaataagta caaaccattc atgtggtttt cggcatattc tccgttcaca tcaatcacta
    42361 aattatttcg tactatattt cctgaaactg taattgtatt aattgtaaaa acagatgatt
    42421 tttcttgttt aacaaatgta ttatctgtca aataatataa ttctccacca tcttgaattt
    42481 tatcccaaac aacaattgaa tttttatcta tatcaatttg acttactgta ttataataag
    42541 ttgaaatatt atttggattg ataaagtatt cagagaattt cacttgaaca tttttaccaa
    42601 tttttacttt atttttaaga ttaaatattt gattgggctt agttgcaata taataaattc
    42661 caatattttc ctcctcaata aatccatctt gaatttcaac aaatacacca tctgaaacta
    42721 atgcattatt tagctctagc aaaaagtctt ttgagtcaga ttctgttaca ttattattgt
    42781 tccaaaatga aaatttctga gattgaggaa agtaagacaa ttccgctata aattgtccat
    42841 caacaaatac caatttatac ttttcttctt gaagtaaagg cactaattca gcatcaattt
    42901 tttggttttc gtttttggta tcaatattaa cttcaaaagg attttggaaa attttattat
    42961 aataagtata tttccattct tcgtgcttca ttgatggcga accaatactg atagcattct
    43021 ccaatgattg ctgttgattt ttatctttta attgtatgtt ttttaatatt tctaaactca
    43081 tttttaaaat ttgaaatcat tatagattaa attcaaatcg tcgaattata ttcaatgatt
    43141 aagctaattc aaattgctca taacctttcg cttctaattc taaggctaaa tttttatctc
    43201 cagtttttac aattttgcca tcaaacatta catgaacaaa atcaggcacg atataatcca
    43261 acaaacgttg atagtgtgta ataacaataa aagcattttc aggcgtgtgc agcttattga
    43321 caccattcgc tacaattctc aaagcatcga tatctaaacc agaatcagtt tcgtctaaaa
    43381 ttgccaattg tggattcagc atcgccattt ggaaaatttc atttctcttc ttttcaccac
    43441 cagaaaatcc ttgattcacg gaacgagaaa taaaatcctt tttcatttct accaattcca
    43501 ttttttcacg catcattttc aaaaactctc ccggtgcaat tggctctaaa cccatagctt
    43561 ttcgctgttc atttaccgca gcttttaaga agttcgcatt actaactcca ggaatttcaa
    43621 ctgggtattg aaatgctaaa aataaacctt tttgagctct ttcatgtgct tctaattcta
    43681 ataaattttc gccattaaaa agaatctctc cagaagtaat ttcgtaacca tcttttccag
    43741 ccaatacgtt acccaaagta gattttcctg ttccatttgg tcccatgatg gcatggattt
    43801 cacctttatt aatttctaag gaaaagcctt ttaaaatttc tcttccatct atcgatacat
    43861 gtaaattttt tatttgtaac atatttgtct attttgattc gcagttcact ttttatagct
    43921 taccgtattt aatttcaata atctaattta ttctcttaat acttattact tgatactaaa
    43981 aaattaccca acactacctt ctaaacttac ttctaataat tttttagcct ccacagcaaa
    44041 ttccattggt aaatgatcca ttacttcttt acaaaatccg tttacaatca ttgcaacagc
    44101 tgtttcctca tcaattcctc tttgttggaa ataaaacaac atatcttcgc caattttaga
    44161 agttgttgct tcgtgttcca cttttgccgt tttattactt acttctaaat atggaaaagt
    44221 gtgtgctcca cattggtcac ccatcaacaa agaatcacat tgagaaaaat ttcttgaacc
    44281 ttctgctttt ttatgaatct ttactaaccc acgataagaa ttttgagatt tcccagcact
    44341 aattccctta gaaacgatac ggcttttagt attttttccg atatgaatca ttttagtacc
    44401 cgtatcagct tgttggaaac cattagtaac tgcaacagaa taaaactcgc caatactatt
    44461 gtcgccagcc aaaatacaac ttggatattt ccaagttata gctgagccag tttccacttg
    44521 tgtccaagaa atcttgctac tattaccttt gcacatgcca cgtttagtaa cgaagttata
    44581 aacgccacca acgccatctt tattccctgg ataccaattt tgtacggttg aatatttaat
    44641 ttcagcatta tcaaaagcaa ttaattcaac aactgctgcg tgcaattggt tttcatctct
    44701 ttgtggagcg gtacagcctt ctaaataaga aacataagca ccttcatcag caattaaaag
    44761 tgtacgctca aactgaccag aaccagctga atttattctg aaataggtac ttaattccat
    44821 tggacatcta acaccttttg gaatataaca gaatgaacca tcactaaaaa cagcactatt
    44881 taaagctgca taaaaattat ctttttgtgg aacaactgta cctatatatt tttttactaa
    44941 ttcgccgtga tttttaatgg catcactcat agaacagaaa ataattcctt tttctttaag
    45001 tgtttcttga aaagttgttt ttacggaaac tgaatcaaaa acaacatcca ttgcaacacc
    45061 agacaaacgt ttttgttcat ctaaagaaat acctaatttt tcaaaagttt taagaatttc
    45121 gggatctact tggtctaaag aatctaattg aggtttaact ttaggtgctg cataataaat
    45181 aatttcttga aaatctactt ttggaaaatg tacatttgcc caatgtggtt ctgtcatttt
    45241 ttgccattct ctaaaagctt tcaatcgcca ttccaacatc cattcaggtt cttccttttt
    45301 ggctgaaata aaacgaacga tatcttcaga aagtcctttt ggtgcaaatt cttgctctat
    45361 atctgtaaca aaaccatatt tataatcggc atttactact tcttctaata tatcatcttg
    45421 tgtgttactc atactaaaaa tgggttaata gggataccta atatttagta caaaggtagt
    45481 ttaaaaatac gtctataaca aactttattt agatttattc caaataaaag cattttatta
    45541 ctgttaagtc ttaataaaga taagtctaac aaataaagat cggttggcga taaattaatc
    45601 taaaagattg tttatgatac gtttaagctc aatttcagca actttagaat tatatcttgc
    45661 taaaataaca ttatacaaag catcttcata actactttga gcttgtttaa tatctaaaga
    45721 tgtagattga tttaaacgat acctttccat cgtaatttgc acgttttcgg cagcaatttg
    45781 gacattttct tcattcaatt gcagcatttt ttctgacaat tcaaaatctt tataagcttt
    45841 aacaattccc gcatcaatat cctgttttaa tttatcgtat tgaatttgtt gtttggcgat
    45901 attaatactt gctgcttgta aatctttttt gaccattccg ccattaaaaa ttggaatttt
    45961 tgcactcacg ccaactgatg gaccataaac acggttaaac aattgaaaac ctgctttatt
    46021 attattttgt acaaaattat agcctgaagt aaagttaatt tgaggcaatc gctgtgcatt
    46081 gatttcctta cgttgaaatt gagcaatttc aatattcttt tttgcagcag ctaataaata
    46141 attagtagaa gttgtagtat ctctgatagt ttgcaaatta ggttgataat ttacttcgat
    46201 attatcaatc acatcaaaag gttcatcagc atttcttcca ataatttgat tcaaattaat
    46261 tttagcttgt tctaaaagtg tttcttgctt taatatatta atttgctgtg tagataaatc
    46321 aacttttgct tgtagtaatg gcgttttatc actatagcct acatctaatt ttttttgtga
    46381 taaattgaca cgctcttcgg agatgccaat tagttcttta gcggctttaa tttgttgttt
    46441 ttgacgaaca atattataat atgcatttaa agtttgagca gccacgtctt gaatttggtt
    46501 tttcaattga atttcaccca atgactgtaa agcggctaat ctatcctttg tagcaaacat
    46561 tttcatacca tcaaagattg tccaatccat tgaaacacca gcatttagcg aatttactgc
    46621 tacccaattt tgtttcacat tttgtccact agcaaatttt tgatttaaat tgttcagatt
    46681 aaaaacatct ccagtattta gattaatttt aggcaacatt cctgcagatg ctttattatt
    46741 taaaaccttt gctacttcag catcattttt tgccaaaaca atatcaaaat ttttagaaac
    46801 agcataagcc actgcttcct caggtgtcat tacttcttgt gcataacttg atgaagcacc
    46861 aaataatatt aaacttatca gtaatttatg aaaaaatttc atcttatata ttttaatgtg
    46921 attgattttt taagactaaa tcggcttcat tttcctttct ttctcttgac ataaaactgt
    46981 atatagctgg aacaataaat aaggtcaaaa ttaaagaaaa tacaatacct ccaataatta
    47041 caatacccaa agatacacga cttgttgctg ctttacccaa tgccacagca attggaactg
    47101 cacctaatgc catcgcaaaa ctggtcatca aaatcggtct taaacgcatt tgtgatgcgt
    47161 gaataattgc ttccaacttg ttcatacctt gttctcttaa ttgattagca aattcaacaa
    47221 ttagaatacc gttcttcgtg accagaccga taagcataat cataccaatc tgactaaaga
    47281 tattcaaggt ttgatcaaaa atccataatg ataataatgc acctgctaat gccaatggaa
    47341 cagtcatcat aataataaat ggatcaacaa aactttcaaa ttgtgctgct aatactaaat
    47401 aaattaaaac taatgctaag attaaagcat acattgtatt agaagaactt tctgcaaaat
    47461 ctcttgaaga accagataat gcagttgagt aggtatcatc taataatttt ttggcaattt
    47521 tattcatttc atcaatacca tcaccaatgg tgtaacctgg tgctaaagct gatgaaactg
    47581 ttgctgattt atatctatca taatgatata attgcggtgg ttttgcaatt tcacgtacag
    47641 ttataatatt atctaacgaa attaaagcac ctgaattatt tctaacataa atatctttca
    47701 ggttgtcagg tttatttctt aaatcatata aagcttgtcc aattacttca tattgtttac
    47761 catcttttag gaaataacta aaacgacgac cactcataga aaactgtaat gtttgagcaa
    47821 tatcttgtac cgaaacaccc atgctatatg ccttatcttt atctataatg atatcaactt
    47881 ctggtttatt aaatttcaaa ttcacatcat agccaacaaa tacaggactt ttagctactt
    47941 cgtccataaa ttttggtata atattttgta gcttttcgaa atcaaaattt ttaatcacaa
    48001 attgaactgg cataccaaat ctaccagaac ctacagcaat cgtttggtct tgaataacaa
    48061 aagcacgacc taaattaaat tgagaaaagt tacgttgcaa atattgagcg atatcattct
    48121 gacttctaac tctttgatct ttatctttta aagccactcg cataaaagca ctattcgctg
    48181 ctcctgaacc agcaaatgaa ggcgacgtaa cacttaaaac aatactttgt tcaggaacag
    48241 aatccacaac aaaatccgtc attctatcca cataatcttg catagcggta taagaagttc
    48301 cctctggagc tgaaaccatt actctgaaag ctcctctatc ttccattggt gccaattctg
    48361 attgtagatt tttactaata aaatatatca tagcaaagca aataccaatc accacaacag
    48421 caatccaacg tattttcata aatgattcta acatttcttt gtagccatca tctaagcctt
    48481 taaaaaaagg ttctgtctta tcatacaacc aagaattttt atgttccttc ccgcctaacc
    48541 aaacattcaa aactggcgta agtgtaagtg atacaaatgc agaaatcaaa actgcaccag
    48601 caacaacaac accaaactcc ataaacaaac tacccacaaa accttgcata aatacaattg
    48661 gtaagaatac aatcgctaaa gtgattgatg ttgctaaaac agcaaataga atttcctcag
    48721 aaccttctat ggctgcttgc attttaggca ttcctttctc tagttttttg taaatatttt
    48781 ctgttacaac aataccgtca tcgaccacca aacctgttgc caaaacaatt gccaataaag
    48841 tcaatacatt tatagtaaac ccaaaaacat acattataaa aaaagcacct attaaggata
    48901 ccggaatatc aaataatgga cgtaaagcca ttactgtatc tctaaagaaa atatatacaa
    48961 ttaagattac taaaacaatt gcaataatta aggtttcctt tacttctgaa attgaatttt
    49021 taataaatac cgtattatct aaagcgatat tgagtttaat atcttttgga acttcatctt
    49081 taatttgctc cattctttta taaaactcat cagaaatctc tacatagttt gcacctggtt
    49141 gtggaacaat agccaaagcg accattgata cagaagcttc tttcaaactt gtttcaaaat
    49201 tttctgcacc taatactgca tagccaacat cttttaatct taccgtttgt cctccaacat
    49261 ctttaataat aatattatca tattcctctg gcttattaat tctaccaata gtattgaccg
    49321 ttaattcagt atttgcacca ggtaattttc ctgaaggcaa ctcaacattt tctttattca
    49381 atgcattttg aatatctact gcagtcaagc cataagctga cattttttgt gggtcaaacc
    49441 acaaacgcat tgcatatttt ttttgtcccc aaatttgaat tgtactaaca ccaggtatag
    49501 tttgcaatcg ttgttgtaga acattggtcg cataatccgt aagttccaat acatttttat
    49561 catcactctg aatagtcata gaaatgattg catcagagtt cgcatcagct tttgaaacaa
    49621 caggagctgc atcaatatct tgtggtaaca aacgaatggc ttgtgaaacc ttatcacgaa
    49681 catcatttgc tgccgtttct aaatcggccg ataattcaaa ctctactgta atattagaag
    49741 aaccttgatt acttgaagat gaaatatttt taatgccttg tactccattg atagcatttt
    49801 cgagtggttc tgtaatttgc gattcaataa tatcagcatt tgcacctgta tagttagttc
    49861 ttacattaat tactggtgga tcgatagcag gatactcacg cacacctaag aatgtatatc
    49921 caattattcc aaacaaaata atagcgatat tcatcacaac agcaaaaact ggacggcgga
    49981 gactcagtgt aggtaaaccc attttatatt aaatttttat tttagataaa ttcctttgct
    50041 tattcttata attattggat agttgaattt gaaactgctt ttctaatttt aacttccatt
    50101 cctggtctaa cttgtaataa acctgtagtc aaaacagtat ctccaagttt taaaccagat
    50161 aaaatctgta ctgaattttc agtacgcaca cccacttcaa catttacaaa ttgagcttta
    50221 ctactatcgc caacaattac cacttttgta gatttatcat ctggaataat tgcaccactt
    50281 ggaattacaa tactattcgg aatttctttt aattttaaat caacttttac ataagaacct
    50341 ggtacaattt tagcattagg actcaacaaa actgcacgca ctttaaggtt tcttgtcatt
    50401 tcatcaacca ctgggtcaat agctgctact tttgctaata ctggctcatt tattccagca
    50461 attttgcaag acattacatc gcctaaattt agtacgcttg cgtatttctc tggcactgcc
    50521 acatcaattt tcaattgact ggtattttgc aaagaagtaa caattgtcgt tgtagaaacc
    50581 attgcaccta agctaatatt ccgcaaccct aattttccac taaatggtgc tcttaattca
    50641 gttttatcaa tttgagcctg taaaatttgc atatctgctt taatattatt caaagtattt
    50701 tcagcagcat catattcagc ttgagaaacg ccctttattt ttaacaactc agccaatcta
    50761 ttaaaattgg tttgagcaat tttaacttga gtttttaatt tggctacttg agcttgtaaa
    50821 tcggcatcat ttatttttaa caacaatgtt cctttagcta caaaagcacc ttccttaata
    50881 tttagatatg taactcttcc agaaacttca ggttgcaatt gtacctcatc ttgagctaaa
    50941 acatttccat tggcactgat gttatctgct aacttagaat attcaaccaa aaatccatca
    51001 actgctaatg ctggcatttt accacccatt gccctcaaag aaccttgttt tgctttagct
    51061 tccttgtttt taccaatttt cacataggct aaaattgcaa taattgcaag tatcgataag
    51121 ccaaatatta atttcccttt tttcatctaa ataataattt ttcgttttaa ttttttattg
    51181 aatcaataaa acgcattaat gcatttttat atgattgatc attatatcta tcttttgatt
    51241 taattttatc aatgagtttg atgtcgatat atccgtgaat aatagcaata taattatcac
    51301 aaaattctaa attcgtttgc ttgcttttca aagcaccatc attcaaacta gaaaaagcta
    51361 acttatatat ctccaaaatt tcttcaagtc tttgacatcc attctgatta catacaacac
    51421 cttgtaaact aaacataact tgatacagtt cagggttggc ttttgtaaaa ttccaccaca
    51481 ccattgcata ttcaaaaagt cgctttgatg ctattttgtg ttgttcgtcg actaactcaa
    51541 attgttgttt aatttgatta aagccatctt cttgtaaggc taataaaata gcttctttcg
    51601 attcaaaata ttgataaata actggagcag tataacttat tttttgacaa atctttctta
    51661 tcgttagatt ttgccaacca tcttcacttg caatagcttt agaaacatct aaaataagct
    51721 gtcgcatttc cactttttct ttatctcttc tagtttgaat agtcatttac ttaactaagt
    51781 tagattttgt aacaaagtta tatttttaat cttttatgct aaagcctctt tagattattt
    51841 aatatttaat tactctaaaa acattattta attgatatat atcatttgat taagaagaat
    51901 tgatttgtca aatactaaat tcaaatttta tttttttgta catttgaaaa cagatgcttt
    51961 ctaatatcta tcatagaatt tacgacaagg tacaatacaa attcagttta atttttattg
    52021 gtatttatat tgtgatatac aattatcaag aaataaatat tggaatatta ttttatcttt
    52081 ttattgcagt tattacgtca attgggattg ctggattagg ttatatttta aatgattata
    52141 aagatttgga agatgatatt aagaacaaca aacgaaatat ttttatccaa ttgaatgaaa
    52201 aagaaaaaat tatcgtgatc gttttattct tgtgtttagc tggtattcct tggctatatt
    52261 ttccttttga tataattagt gcgttgctta ttttattgga acttatatta ttctatattt
    52321 atgcgtttcc gcctttacgt ttgaaagaaa aaggattttg gggtattctt tgtgatagtt
    52381 tgtatgcaca agtaattcca tgtagtttgg cagcatatac tttctataaa atccaaacac
    52441 ccaaacaaaa cttttcgatc tattttattc tgatagtttt actctggttg tttattattg
    52501 gcattagaaa tatccttatc catcaaattg atgattttga aaatgaccaa aataccaaaa
    52561 caaaaacttt tgctaccaac attggaaaaa agcaaactat gaaaatcgtt tataatagca
    52621 tactaccttt agaaataatc ctttttacca tcttactcta tcaattacca caaaatggtc
    52681 tatttattat tgctttgtat attatatatt gtatcgcaat ttttctaatt aataaagagc
    52741 atttcaggaa gcattttgct cattatgtga atcaaagatt actcaatgaa ttttatgaaa
    52801 tttatcttcc tgttctgtta ttaatcatct attatatatc tggcatacat tcattatata
    52861 tcccaatttt tcattttgtg ttatttgctc caatatatct aaaaagtttt ttagcattta
    52921 ctaaaaaata cttctaaagt tttgttttga tatgattttt caaaatctcg atagaatgtt
    52981 ctaaactttc tcttgtttta ttattaataa cgatatcaca atctgcttta tatggaaata
    53041 tatacttctc aaatgctgga taaacatgat tttcataacg atacaacaca tcatcaagtg
    53101 gataatttcg ttctgcttgg tctctcacaa ttcttcttgt caattccaaa taagatggaa
    53161 tatcaataaa taattttaaa tccattaagg cttcaatttc tctaaaatga tagacaaaaa
    53221 gtccttctat aatcaatact tttgcaggtt tgtagataat tttctcagca gttgcaagat
    53281 cattattaaa agtatattcc attaattcta gagtctctcc attgagtaaa cgcttaacat
    53341 ctttctcaaa ttcatctttt ctaaatgaag taggtaaatc aaaattttga tgattatttt
    53401 tatctacaac ttgttcttct ctttttttat aataattatc ctgactaagc aagcaaactt
    53461 catcttctga aaagaatttt ctcaaatttc tgacaaaagt tgtctttcca cttccacttc
    53521 caccagaaac gccaataaca taagttttat tcatgttagt aatattacga cctaaaatta
    53581 aaatacaaaa attatcttac gtatttttgt aaaaaattat ctctatgcaa ttattagatg
    53641 ggaagaagac tgctgcatct atcaaagatg aaatcaaaca aacggttgat gctattaaat
    53701 tacagaaaaa aagagttcca catcttgcag caattttagt cggagatagt actgccagca
    53761 aaacctatat caataataaa ataaaatctt gcaaggaagt tggttttaaa tctaccctat
    53821 tacatttgga aaaagatata accgaaaatg aattaattgc tcaaattaat aacttaaaca
    53881 atgacaatag tattgatggt tttattgttc aactaccatt accaaaacat attaatgaag
    53941 agcgtgtttt attaagcatt actccagaaa aagatgttga tggttttcat cctatcaact
    54001 tagggaaaat gatgattggc ttacctgctt ttatttcagc aacacctaaa ggtattgtag
    54061 agattttaaa aagataccat attcaaactt caggaaaaca ttgtgttgtt gtaggtagaa
    54121 gtaatattgt aggcacacca attagtattt tattaggcaa aaaatcagaa gctggtaatg
    54181 caactgttac attatgccat agccatacta aagatttaaa gacatatacc ttacaagcag
    54241 acatcatcat tgcagcgact ggtaaaccaa atttaattac ggctgatatg gtaaaagacg
    54301 gtgttgtgat tattgatgtt ggtatcaata gaatagatga taatagtgaa agaggttatc
    54361 atttagtggg cgatgtggac tttaaaaatg tttctaaaaa aagtagttat attacacctg
    54421 ttcctggcgg agtgggttta atgactgttg cttctctttt acaaaacaca ttagctgctt
    54481 atcaagcagc atattatccc aaagatttta tagaagaaaa tttattgtaa tgaataaaga
    54541 tttaattttt aaaaatgcta ttggtgctgc tctaatttat ataattctga aagtagtgac
    54601 tttagctatt ggaatagaaa tgcagtatcc aatagattgg gttatatttg caattattgt
    54661 tggcttattc attttttttg gaaaactata tagtcaatct attaatgatg aaagcaatat
    54721 gggtgatata ttgctgcatg gttttgttac ttcattagta tatatatttt tcatttctat
    54781 atttaccgta atctatgtca aatatattga tgtaaattat tatcaaaaaa tatttgatat
    54841 ggctgaaaaa gaactgcaaa attatagtat ggaaccagag aaaatggagg caatgaagga
    54901 actttcaaaa aagtatatga ccattcccta tctgttgctt tctactttcc tttccaatat
    54961 catattatgt aatattggaa attttattgg agccgtaata tttagaaata aaaattaaat
    55021 acaaaattcc tttatgaata tacatacact tgccttacaa aactttccag attatgaact
    55081 tatagactgt ggtaattttc aaaaactaga aagatttgga caatacatca ctatccgacc
    55141 agaaccacaa gctgtatggg attcaaaatt aaatatagat gaatggaaaa aacttgctca
    55201 tgtaagtttc gtatctaaat caagttcctc tggagaatgg caaaaactaa aaaccatgcc
    55261 tgataattgg aatattaaat atcaatttag agataaaaaa tcaattcaat ttaatttggc
    55321 attgacaaaa ttcaaacatg ttggtgtatt tcctgagcag tgtgtgaatt gggattttat
    55381 ttatgataga agcaaacaaa ttatacaaca aaacaatact tgtaatgtac taaacttatt
    55441 tgcatacact ggtggagcaa gtttggctgc acgcactgca ggtgctgatg tttggcacgt
    55501 agatgccatc aaacaagtgg taagttgggc aaatagaaat atggaaagct ctaatcttga
    55561 caacattcgt tgggtcgttg aagatgcttt aaaatttgta aaaagagaag taaagagagg
    55621 aaaaaaatat caaggtatta ttttagatcc acctgctttt ggacatggac caaatggaga
    55681 aagttggaaa ttagaaaatc aaataaatga acttttgttt gatgtcataa aattacttga
    55741 tgagaaacac caatttatgg ttttaaatgt atattctttg ggcttttcgt cattaattgt
    55801 agaaaattta gttcagagta atttggataa aaatggcgta aaaatatcca atatggaaat
    55861 aggtgaaatc tatattccat ctcaacaaaa acagaaactc cctttgggcg tttattgtag
    55921 gttttaacta caataaacgc caattagatt actttgttgc ttttttctta gcaggtgctt
    55981 ttgatttata ttcatcgaca actgcaacag ctctactaag gatattaaat cgctcagcat
    56041 tcaaggtgta atagattttt tttccatctc tccttgtgtc cactgcttct acgcctctta
    56101 acatttttaa atgttgtgaa gtaatagatt gctcaatttt caatgtgtta taaatcacat
    56161 ttacatttac ttcacccttt tcgttgatta actttacaat ttttagtctt aaagggtgcg
    56221 ctactgcttt tagcaactca gcagcctttt ggatttggtc tgagtttagc tctaattttt
    56281 ccataaaatt taatataaaa agaataatta atacaacaaa tatagtaaat aaatgctatt
    56341 aataacaaat aaatttatca aacattaaat aatcaaagaa aataatatgc cttaaatcac
    56401 ttttttgtag aataaaaggg ctttgtatag cttggttcaa aatacgcaac atcaacaaaa
    56461 ttagttagtt gaaaatagtg atttgctaat tctatcatat cttttgcagt atattcaaaa
    56521 tcttgatgaa aaataaattt atgatttagt aacttttgtt gcaaataaat atgaatcttt
    56581 tcagaagcat ttccaataaa aaagtattga tttccatttt caaaaaaact taaaaaatca
    56641 tcatctaaaa tacatggctg tgctttatat aattcttcta atttggaatt ataaacagca
    56701 aagtaaacct catcacgtcg agcatcgata tttggaacta ccaaaaagtc tgaaatattt
    56761 tcactaatta ttgctttatt tgtcataatt gctaaggttg gcagtgctat aattggaatg
    56821 tttaatgcat aagcaatacc ttttgcaaca gctgcaccaa tccttaaacc tgtataagaa
    56881 cctggcccat tacttattgc aatggcttgc atatcactca acttcaaatt tgcttgactt
    56941 aatgcctcat ttattaactc attaatttgt tctgaagcag tattgggtaa taaacattca
    57001 ctataaccca acaaatttgt accatttgca agcgcaactg aacaagtact caaagcagta
    57061 tcaatacaaa taatatttac agacattatt tcttagcaga taaaatttct ttgtattgaa
    57121 tgtagtttaa taaaatttct tttgctttag caacatcatt gtcattgatt aaactatttg
    57181 caattctacc tttttcaaaa tagtaacgag caacgatttc atcttccaat aactttttga
    57241 tttgttgctc atatttaaaa acatcttgtt tcttatctgc atcaatttta tcgtgtaaca
    57301 aacctaatgt acttttaatt tcagcagaat aattttcatc tttaattaaa tcattcaatt
    57361 tatctaatgt ttcttcagtt tttgaagtat aatcatattt tttaccttct agaaacttca
    57421 cgaattgctt gtactcttct tcattcatac tgaattttgt actatctctt aaaactttat
    57481 ggtcatattt atattgagta gcataatcaa aaatcagatt attcacatat aaagcaatcg
    57541 taataggcga cattacttgt tcttcagttt taatatctgg ttcaatacct gcaccatctt
    57601 tgaccaatct accattttta gttttaaaaa cacttcgcaa tgaatcaggc aaggcactag
    57661 cttttccatt ttcatcacga tgactataat ctatacgttg aatacatctg ccacttggaa
    57721 tataatattt agaaattgtc aattttaatt ttgatttata acctacgggc ttcgtaacct
    57781 gtactaaacc tttaccataa gaattttgtc caataacaac gcctctatct aaatcttgga
    57841 tagaacctga aacaatttca gatgctgaag cactattttc atctatcaaa atagccaatg
    57901 gaatctgtaa atcaactggt gaattttgag tggtataatc ataattattt tcaggtactt
    57961 ttccttttgt acttaccaca atatttcctt tatcaataaa ggcattgcaa atatttaccg
    58021 cctccattaa aagtccgcca ccattacctc ttaaatcaaa gaccacacct ttcaaatttg
    58081 gatttttctt taaattcaac aatgcatctt gtacttcttt tgatgcattt tctgtaaatg
    58141 aaatcaactt tatataacca atattattat ccaacattcc agaataagca acgctattta
    58201 tcttaatttc ttctctattg aatgtgtaag tcttaatttc attacttata gcttttctta
    58261 ctttaacagt caaacttgta ccaggttcgc ctttcaaaag ctcacttaca tctgatgaat
    58321 tataatcttt acaaactttt ccatcaattt caataataac atcaccaggt tctaatccat
    58381 ttttttgagc aggaaaccct tcataaggtt ctgtaatcat cacctcatta ttgatacgac
    58441 caattctggc accaattcca ccatattttc cagtagtttg catttggtac tcatcaatat
    58501 ctgctccagt aatataatta gtaaatggat ctaaattttc caacatttta ttgatgccat
    58561 tggtcatcaa tgtatttgga tcaatatcat caacatacga gttgtttaat tctttatata
    58621 cattggtaaa tagctcaata tttttggcca tatcaaagta tttattattc gatgtagtgc
    58681 taggcgtatt ttcttttgca agaatcgaca aacttaaaaa caaactgaat aggagtacaa
    58741 aaaatctttt cataaatcgg gtataattaa gtaactaagc taagtaaaaa aaacagttta
    58801 taaggttaaa aaaattaaat aaaaaaatca aatactaaac atcatcttca taatcgaaag
    58861 tatgttcttc taaaaattta gtggtaaaat ttcctgcaac aaaatcctta ttattcatca
    58921 atttttgatg aaaaggaatc gtggtgtgaa taccttcaat tttcatttca gctaacgcac
    58981 gtttcatttt ttcaatcgct tgttggcgat tgggtgcttt tacaatcaac ttagcaatca
    59041 tagaatcata aaatggcgga atgctatatc ctgaataaac atgtgtatca atgcgaacgc
    59101 caatacctcc acttggaata taattaataa tttttccagc tgatggttta aaattatcta
    59161 aaggattctc cgcattaatt ctacattcaa tggcatgtcc acttggagca tagacactct
    59221 gttcaatttt ttcgcctgct gcaattaaaa tttgcaatcg aatcaaatct aaatttgtta
    59281 cttcttcagt aacaggatgt tctacttgaa tacgcgtatt catttccata aaatagaaat
    59341 ttttgtgctt atctactaaa aattctattg ttccaacgcc ttcatatcgt attgctttta
    59401 ctgcattaac cgcagccact cctaattgtt ctcgcaattt atcatctaca aatggtgatg
    59461 ggctttcctc caccaatttc tgatgtcttc tttgaataga acaatctctt tcgctcatgt
    59521 gacaagcatt gccaaattgg tcgccagcaa cttgcacttc tatatgtctt ggttcttcaa
    59581 tatatttttc taaataaacg ccatcgtttc caaaagatgc tccagcttcc actctcgctt
    59641 gttgcatatt gtgttcaaat tctgattctt cccaaacaat acgcatacct tttccacctc
    59701 cgccagcagt tgcttttaga ataataggat aaccaacttt cttagctaat tgaataccct
    59761 ttttcacatc tgtaattaag ccttcagaac ctggaactac tggaacttta gctttaatca
    59821 tggtttcttt tgctgtaatt ttatcgccca tcgatgcaat ttgttcgggt gttgggccta
    59881 taaatttgat attattttca gcacacttac gagcaaaacc ttcattttcg gctaaaaaac
    59941 cataacccgg atgaatcgca tctgccttgg ataaaatcgc tgcttgaata atagcatctt
    60001 gatttaaata agataattta ctttgtggag caccaataca aaatgattca tctgcatttt
    60061 tgacatgcaa actttcttta tcagctgtag aataaactgc tactgttttt atccccaatt
    60121 cttttgctgc tcttatgact ctcaatgcaa tttctccacg attggcaatc aatatttttg
    60181 taattttcat tctaatattt tctaattaaa ttaagctatg agtatttata aattgaaatt
    60241 actctcctcc ctcaaatata gaaaaatata ttaaattttt agattaaaaa ctaggcaata
    60301 gtttatctcg cataaaaaag cctcacaaaa atgtgaggct tcaaatagga ttttatttaa
    60361 ttacctagtt aataaattac tttattgaac tggcgaaagt gtaacctgaa tcacatcatc
    60421 tgttgttaaa tacttagttg ctgcttgttt tatatcttct aaactcaatg tgtcgatagc
    60481 ttgtaaatat tgatttaatt tatgtatatc ccaattatat ttatcagcat aaaccatttt
    60541 tccaatccaa taatcatttt ctttttgatc tgtttcaaat tctcgcttat aagtttcttt
    60601 tactttattc aacttttcta aagttgtgcc attttttaca aaatcatcaa tttcatcata
    60661 cgtagctttt atcaattctt gagcttttgg tggatctgca ttaaaagaaa tattaatcgc
    60721 aaatttttct ttcggaattt tacttacaac accttgaaca cttacaccat aaacgcctcc
    60781 tttttcttct cttaattctt ctctcaatac aatacttaaa acttttacca ttgcattaaa
    60841 tttcaaagcc tcagcatcgt tataatttaa agttccgtgt tcattgatat tcacaaaagt
    60901 tttaggcgta ttgcccattt tcaaattagt ttctacttta ccctttggtt tttctacatt
    60961 cgggtctttc cacatttctc tttttgtaga atctccaggt aaagaagcaa tgtatttagt
    61021 aattaaagta tcgatttgtt ttgcatcaat atttccaaca aaataataag taaaatctgc
    61081 tgcattccaa aatctttcat tatagaattt gaaagcattt gcatagttta cttgatccaa
    61141 tgtttctttc gttggcaagt ttcttctaat attattatta tagtttattt cagacaattt
    61201 tttcataaaa taatactgcg gattactata caaattatca taaatagcat gttgtttttg
    61261 cataaaagtg gtaaaatctt ctt
//
LOCUS       DAOQCW010000002        58203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_182_length_58203_cov_5.949938, whole genome shotgun sequence.
ACCESSION   DAOQCW010000002 DAOQCW010000000
VERSION     DAOQCW010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 58203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 58203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58203
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182_length_58203_cov_5.949938"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..570)
                     /locus_tag="PLS16_00300"
     CDS             complement(166..570)
                     /locus_tag="PLS16_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNJ59545.1"
                     /translation="MEKVLVDANIIMDLLEKREEFYQEAQELFTLSDKKNVKLFVSAL
                     TLANVHFLLYKHLKMEARKVLAKFKVLVEVLAVDDKIIDLSLTSDLKDFEDAIQYYTA
                     IENDMDIIITRNKKDFKNVNLPILTAKEFLNR"
     gene            complement(570..815)
                     /locus_tag="PLS16_00305"
     CDS             complement(570..815)
                     /locus_tag="PLS16_00305"
                     /inference="COORDINATES: protein motif:HMM:NF039417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HNJ59546.1"
                     /translation="MNTKLTLNINKSIIESAKDYAKENRVSLSKLIENYLNSIIKKED
                     KEIKVSPLVESLTGIIPTQTEEKNNDDYYEYLSKKYS"
     gene            complement(1009..2157)
                     /locus_tag="PLS16_00310"
     CDS             complement(1009..2157)
                     /locus_tag="PLS16_00310"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNJ59547.1"
                     /translation="MSKQILIIGGAGFIGSNLALKLIEKGHQISILDNLHPQIHGANP
                     EQTSPLYQAIKNKVRFIHHDVANDHPTIWKDALKNQQIIVHFAAETGTGQSMYQIKRY
                     NQTNVMGTSNLLEALMNFEHQVEKVVVASSRAIYGEGKYFSPTINQFVYPKHRNEQDL
                     LNGDFECKCPITNQEVELCATDEMSFVQPNSIYGLNKYEQEKAIEITCSALNIPYVAL
                     RYQNVYGVGQSLSNPYTGILAVFSNRIREKQPILIFEDGKESRDFVYIDDVVQATLLS
                     IENNEANNKAINVGTGKATSVIEVAQELIKNYEIEVDIQITQKFRKGDIRHNFADISL
                     LKNSLNFEPKISFQEGIKKYVDWVKTQPLVKNNFEQSLNEMQAKGLYK"
     gene            2247..3083
                     /locus_tag="PLS16_00315"
     CDS             2247..3083
                     /locus_tag="PLS16_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ59548.1"
                     /translation="MKKIDLIIPGSKDKPITLDIQYQESDNNIKPVVLFMHGFKGFKD
                     WGHFNLIAKTFVENGFVFVKLNTSHNGTTPEHLEDFIDLEAFANNNFSIESDDIDMVI
                     QFIETNIHHYFGDKNQLHIIGHSRGGGLVLLAGHRNPSIKKIVTWATVQNLKYFFDYM
                     DIKKWAADGVAYIPNSRTKQDMPFYYQMYENYLFNQEKLDVEKVVSETEKPILFIHGS
                     GDIGVPQKYSEYLHHQNPNSSLEIIEHADHTFGGKHPWIADELPGHSKILVQKTIDFL
                     RK"
     gene            complement(3099..4649)
                     /locus_tag="PLS16_00320"
     CDS             complement(3099..4649)
                     /locus_tag="PLS16_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HNJ59549.1"
                     /translation="MDTKILWADDEIDLLKPQILFLEQKGYKISAVSNGYDAIDKCNE
                     EDFDLVFLDENMPGISGLETLSKIKFLHNDLPVVMITKSEEENIMEEAIGSQIADYLI
                     KPVRPNQVLLSIKKIIDNKRLVSEKTSVSYRQQFQQIMMELNDDLDAEAWAELYKKLI
                     YWDLELDKSSNTDMLDVFEMQKVEANKEFYKYIQKNYTKWLQKPDAGTPAQSHTLIKK
                     KVLPILDNDVPTFFILIDNLRYDHYKMLEPILKERFKKVSEDFFYSILPTTTQYSRNA
                     IFSGLMPSEINKKYPDKWFFDDENEGKNLHEAFFLEEQLKRNGLNIKSSYTKITNHKD
                     GKELEDNILNMMHNQFNAIVYNFVDMLSHVRTEMEVLKEIANDEAAYRSLTLSWFEHS
                     PLLSALEKLQGKKARIVITSDHGTTRVKTPSKVVGDKNTTTNLRYKTGKNLNFNPKEV
                     FEVKNPDEAFLPKSNVSSSYIFAKEDYYFCYPNNYNYFVNFFRNTFQHGGISLEEVIV
                     PVAIFETK"
     gene            4757..5941
                     /locus_tag="PLS16_00325"
     CDS             4757..5941
                     /locus_tag="PLS16_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNJ59550.1"
                     /translation="MNKRKIFNDPVYGFISIPFDTAFDVIQHPIFQRLRRIQQLGFSS
                     LVYPGATHTRFHHSLGAMHLMTRALDTLKKKDIQIDTEEIEATVLAILMHDLGHGPYS
                     HALEHTLVDGVDHEVISKLLMLELEKEFGDSITKAIQIFEDKHPKKFLHQLVSGQLDM
                     DRMDYLNRDSFYTGVSEGVIGYDRIINMLNVEQDNIVVEEKGIYSIEKFLVARRLMYW
                     QVYLHKTVLCAEILLKSIFNYIRNFGIKLDFDTPLNLFIENKFDKQASIEWLSDFCQL
                     DDTDIVFALKHLSKKKDPILQKLIDALLYRQLFHIEFADVQRINIIYERLKLKYSAEE
                     MDYLVFRGSTSNNMYNTEKDNIFIQMKSGKTIDVASISEQWNLDKISNPVVKYYIIYP
                     KN"
     gene            5967..6299
                     /locus_tag="PLS16_00330"
     CDS             5967..6299
                     /locus_tag="PLS16_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HopJ type III effector protein"
                     /protein_id="HNJ59551.1"
                     /translation="MTLEIIQQIEAGTANFKDVIAYIDSKYSHTPTAFKNGNQENAAD
                     QNQGSAKVFAFGKLNELSEADTLKLFVEHYQSVLATPSGTDHQNIRQFMQNGWSGISF
                     SGAALVLK"
     gene            6423..6653
                     /locus_tag="PLS16_00335"
     CDS             6423..6653
                     /locus_tag="PLS16_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HNJ59552.1"
                     /translation="MAKRTNHVVPSSSGWSVKKSGSSRASKVFDNKEMAIDYARKISI
                     TERTELYIHKKNGTIQDKRSYGNYPNAPKNKN"
     gene            6653..6952
                     /locus_tag="PLS16_00340"
     CDS             6653..6952
                     /locus_tag="PLS16_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59553.1"
                     /translation="MKYENSVFINCPFDDSFINDLLKPTLFFLIYNGYNPRLSLEISD
                     SGQVRLEKITEIIKNCKYSIHDLSIVKAKKVNEYARMNMPFELGIDYGLRNCVDK"
     gene            7248..7454
                     /locus_tag="PLS16_00345"
     CDS             7248..7454
                     /locus_tag="PLS16_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59554.1"
                     /translation="MKKLEKEYNLFKYKECNCEKDAWEEIVVQQDDHFGGKTVIYFHC
                     SDCGIDFRVEDFYTHEELHPKIIE"
     gene            7480..7605
                     /locus_tag="PLS16_00350"
     CDS             7480..7605
                     /locus_tag="PLS16_00350"
                     /inference="COORDINATES: protein motif:HMM:NF022902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59555.1"
                     /translation="MKQGNFKIGRITMQRKGGDNGRETAKMLQFKINPAELFEVE"
     gene            complement(7626..8432)
                     /locus_tag="PLS16_00355"
     CDS             complement(7626..8432)
                     /locus_tag="PLS16_00355"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HNJ59556.1"
                     /translation="MQPLKTATFIGSGNVATHLAQALFHKGIKIKQVCSRLMENATVL
                     ATKVNAEPIDHIDNLDETVDIIFCCTKDELIGKISKRIARKKKIKSIVHTSGTVGLEV
                     FSDFENKFEAVGIFYPLQTFSKLNAVDFSEIPIGITTKNNFFEAELINLASKITQRTF
                     VINDEDRQHLHLSAVLVNNFVNHLFAIAQQYCKDHQLDFNLLKPLIHQTINKIDVVPA
                     IDAQTGPAKRKDFNIIDKHLSMLDKDILTKNIYQSLTESILKMYYKNIKL"
     gene            complement(8419..10842)
                     /gene="ccsA"
                     /locus_tag="PLS16_00360"
     CDS             complement(8419..10842)
                     /gene="ccsA"
                     /locus_tag="PLS16_00360"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNJ59557.1"
                     /translation="MDIYSTGSDLIFGKIGHLFIIITFISVIVSTISYFIATQYSNNS
                     NTASWIGLGRKAFIVHTISAIGIFSILFILLFTHSFQYKYIWQHSSQDLPVFYIFSAM
                     WSGQEGSTMLWIFWTAIVGCVLIFRAKSYETGVMTFVSLSQVMLCAMLLGIYIFDYKL
                     GSSPFALLKDTMDIPIFKMNPNYIPGDGTGLNPSLQNYWMVIHPPTLFLGFALTTVPA
                     AFAFASLWKKEYTQFAEAVLPWTLIALMILGAGILMGGAWAYEALNFGGFWAWDPVEN
                     ASLVPWLILAAALHTVIVYKHTGHSLSITYAFFLMAYLLVLYSSFMTKSGVLGDASVH
                     SFTDLGMSGLLISTILVFLIPSLGLYFYRSFKKQIPFKETEEATSSREFWLFIGALLF
                     TLSAIHITGLTSFPVFNKLPFIKNKLAPPSDIKSHYNHVQIWLAMFAAFGTAMVQFLK
                     FTKTNLKGFFKNFIWSLAGGIIVTIGIVAYYKINRIDYILMLVAGWFTIFSNINYIKN
                     VMKGKVKVSGGYVSHIGFGLMMVGIVVSQGEQEVVSINRYGINYGSGFTADESAENIL
                     LYQHEPQVMGKYKVTYLGDSIHDKNIYFNVLYEQMNKKNEIVKRFTLHPNILLNQKMG
                     NNPVPSTRKTLESDLYTHITMAPLKEDGTPVDSIVEEKHTLGVGDTLMLSRAFAIIEG
                     INTHAEVKGIEKKPNDVAVGVKMRYVDLDTQYTIEPVYFIRDMMATSIEAEAHDHKTK
                     IAVTRIIPESNKFEFTVEETLHKYIIMKAIKFPFINVLWLGILVMVSGIFISMQKRLS
                     DNKRKYAAA"
     gene            complement(10846..11226)
                     /locus_tag="PLS16_00365"
     CDS             complement(10846..11226)
                     /locus_tag="PLS16_00365"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HNJ59558.1"
                     /translation="MKKTHIIALVLVAVMIGVLVTLLGDFSRYETFESNYAKKGKEIN
                     VVGELNKVKGITYEPTKNPNIFSMYIVDAKGVEKKVIVKKAKPRDIERSEKIVVIGKM
                     HDDTFIADDILMKCPSKYQEKTNS"
     gene            11381..11839
                     /locus_tag="PLS16_00370"
     CDS             11381..11839
                     /locus_tag="PLS16_00370"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNJ59559.1"
                     /translation="MEKKHLLFIYHSQSGHTAQLIEKCKQGASKEEVEIRHLKAFDTT
                     LEDVLWADGIVIGTPEYFGTMSGALKDFFDRTYDDAKKAEINKPYALIISAGNDGTGA
                     ERQVQSLTTSYTMKRVLDTIIVNETEFDSKLIDVEELGQTFAAGLALGIF"
     gene            complement(11901..12332)
                     /locus_tag="PLS16_00375"
     CDS             complement(11901..12332)
                     /locus_tag="PLS16_00375"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNJ59560.1"
                     /translation="MNERVAFFRRGIGNDLWKEHYPIIKFLNGSIVEVNEGQLIFEFT
                     ILETQLNPNGILHGGVMATMLDELMGGATWTLNKPYPFATINLQVDYLSPAKVGEVIR
                     GEANVIKAGNTVVHTEAKLYNSKNMVIAKATSNLVVMKPKS"
     gene            complement(12340..13941)
                     /locus_tag="PLS16_00380"
     CDS             complement(12340..13941)
                     /locus_tag="PLS16_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNJ59561.1"
                     /translation="MSFDKEIEKRRTFAIIAHPDAGKTTLTEKFLLYGGAIQTAGAVK
                     SNKIKKSATSDFMEIEKQRGISVATSVMSFEYKNVLVNLLDTPGHKDFAEDTYRTLTA
                     VDSVILVVDCVKGVEMQTEKLMEVCRMRKTPVIIFVNKMDRDGKDPFDLLDELEQKLD
                     IKVRPLSWPINKGTFFKGVYNIFDKNLNLYRTSGTTVEEEFVAIQDINSPELDKQLDK
                     RDADKLREDIDLIEGVYDDFKVTDYLNGDIAPVFFGSGVNNFGVRELLETFIRISPVP
                     RPRNAGDRIIYPTEPKFTGFVFKIHANIDPNHRSRIAFLRVCSGKFERNKFFYHTRSN
                     KNLRFSNPVTFMASDKNIVDDAYAGDVIGLNDTGNFKIGDTLTEGEKLTFKGIPSFSP
                     EIFKELVNTDPMKTKQLEKGVSQLMEEGVAQLFTTHLTNAKIIGTVGELQFEVIQYRL
                     LHEYGASCTFKPMQYYKAFWISSDDANEINQFIKTKYNYVAFDKEENPVFLATSEWIY
                     TLTKQDYPKINFHTTSEMEQDEIVK"
     gene            14091..15017
                     /locus_tag="PLS16_00385"
     CDS             14091..15017
                     /locus_tag="PLS16_00385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNJ59562.1"
                     /translation="MKFKIFGVFVLIMMCFVSIAQDVHFSQAPNNYIYLNPANTGFGP
                     KTNRVVGLYRDQYRTAFATFSTTFMSYDRRLVAWGNGWQLGGGVDFLYDKVGYGIIST
                     FSPNISLAIGKYFNQGRQLLNIGISSGFAFKKLNYSNLTFDNQYIPLIGFDPNSASGE
                     QLDNKHRIYPNLNFGINFSTALGDYSKLDIGAALANLHQPNQSFLANSVSRLPARYTS
                     YIKAFIGFANNEKWSLQPAFVYNFQNKAQNILVIANVETKFKETNRGNYLGLGFGAGY
                     RIQDNDAMIANISVLLGDLRIATSYDFNISKY"
     gene            15249..16934
                     /locus_tag="PLS16_00390"
     CDS             15249..16934
                     /locus_tag="PLS16_00390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNJ59563.1"
                     /translation="MNFRIIIFSIICIFIYQLGFSQDPHFSQIQYNPIYLNPAFAGFA
                     KKDNRVVGVYRDQWRTAFVPYSTTFGSYDRKFKQWEKGWRLGGGISFLYDRAGEGILS
                     TFNPNLTLAVGKYFNNEKQLLNFGITSGITIKSLNYSKLTFDNQYVAGSGYVPGSTPG
                     ESFSNNNVSYPNFTIGLNFTTKLGEKSSLDIGGAASNVHQPNQNFLYFSESKLPARYT
                     AYAKALVGLGKSDKWNLQPAFIYNFQNKANNILVNAIAETKFKENNKGKTLGLGFGAG
                     FRVQDVDAAIAYISVLYDDLRIGASYDINLSKYKGATNTMGAFELLLSYEWGNSKKKK
                     CTPDTIKIKEFEILRDTVYLTDTIIQKDTVIQIKETTVTPEQIQKWNESLSKFLPAIA
                     YFDNDYPDPKSQLTTTNANYKDLYENYISKRSKFVDYIGEEETGKLFDTIGTEFNKLI
                     QIYGALKEILDRGKDVKLQFAGYTSPLSTVGYNQKLSSRRIESVMNYLFSLENGYLKK
                     YLDNKQLTFEEVPLGKSKAPESISDKADDYRNSVYGRNASLQRKVEIKAIYIK"
     gene            complement(17198..20092)
                     /locus_tag="PLS16_00395"
     CDS             complement(17198..20092)
                     /locus_tag="PLS16_00395"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNJ59564.1"
                     /translation="MRKIIVSFVWVCLLFINLKAQQLNIPNGKLIPAPKVNGTLDDLT
                     DFAQRVQVPFMMPDSTILYTDIYLPILQDSLTFDVTIPIINQDIKLTVLGKGFQYIIY
                     DSINGQLNPNPYQLPMILERTPYNKNGGDVMIGSAMALLGYSAAVQDMRGRYTSQGAY
                     LPLYSDSWKKTPYHNNNHLLDITSPDDPRNGNNHEDGYNTIEFIKNNLVRKFDLDKNG
                     TLETTDLVYNGSIGMFGASALGYNQHQAAAAHKIDPTQPGLKSLFPIVGPLEFYKSTG
                     YHNGCFRNMLVKGWLKGQIYDLEDDKMSIDQGINDSIHTAKDYNLPNKFVAAETAVDH
                     FSTYKYPGSTPGYFPNSINRDNMDGSVAMVDENGEGDKNGKFSRYTNMEVASFQVAGW
                     WDLFVDGQIETNNFARQYLTHKKNLQKIVIGPWAHQTIGSRKTGDKTYPVNVTDFTKV
                     DISNFGDDLDVGSIAESELLTWFRYNLNYFGQNNVGEPKILIPKSNTWQTVVGDLKVQ
                     FPAKDYKMKFIDLLNFLLATDGLKQVPVSIKLGNLNPLNININIPKLDDAIVKGISSS
                     KKLESLKKLEYKDIPAYRFYVVGPSDNQGMSLGAGNYWMGADKFPIDDVVWQSLYLHR
                     NNGLSFEMPSEDEGLVSYTDNPNNPIITVGGNNMLVKTPQGDRDSQGQMNLADPNFSS
                     FTMDREGVIKFETDVLSDSLSIIGFPKVKLYAKSNPLNVQNGETDCDFYVRILDVYPD
                     GSEFYVVEGGINARAREWAKSIAEGNENINAPFTNINVGQIYEFFFEMYPIAYTFAEG
                     HKMKVLICSSNYPRFQANPNTPIEDGTFFRRAPNDGKTNIYKGVEYTPRIAVQSVAIS
                     DIYDSQIILPIYGATKVISGIRNNPMKKPNFDFNLFPNPSQGEFSIVMDKNGGYNVNI
                     YNLLGQKLINKDIQDQAYFDLKGYPKGNYVMEVIDTKDNSKKQTKLFSIF"
     gene            complement(20276..21376)
                     /locus_tag="PLS16_00400"
     CDS             complement(20276..21376)
                     /locus_tag="PLS16_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59565.1"
                     /translation="MNHKDKLLEIYNRSSIVTDIKDVNKKTKDFIKSIGTKINTQKGV
                     FTVLVTLITHKIINPKQDIRKHQTSMDGGFSGRSIDTVFITPTLKQLKLPSMAESGWL
                     TRSLEQPYPYNLDYNGKISNKIVKDAFLNVLDFVERNPKSAKNVLRLLLNEAINSKND
                     ALVDIIPLENPETLTIEKIISALDEHFRTKYATSGGSKLPVLAFFAIYKSLIKEIERY
                     KSCTLAEMGSHTASDRTSKSAGDIEIFKKKELFEAIEIKLDKAIDITMVRIAIEKIAR
                     YNPNRYYILSYLGIKETDKLEIDDLINQLKFEHGCQIIINGLLPTIKYYLRLIDSLDE
                     FVKNYNELVQNDNEIKKIHKLKWNELIDKYLK"
     gene            complement(21315..22355)
                     /gene="dcm"
                     /locus_tag="PLS16_00405"
     CDS             complement(21315..22355)
                     /gene="dcm"
                     /locus_tag="PLS16_00405"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HNJ59566.1"
                     /translation="MKYKFIDLFAGIGGFRKGFEDAGFKCVFTSEINKPCQEVYKNNF
                     GEKPFGDITKINPTDIPNFDVLLGGFPCQPFSISGKKMGFEDTRGTLFFDICQIIDKK
                     QPNVVVLENVKHLVHHDKGRTFTVILKSLMDLGYNVTHQILNAKDFGLPQNRERIFII
                     ATKKYYFNFSKLEKKDKVILRDFLDTEGEFEFLEKSEYTLIETPKKQESGLLFVGYRN
                     KNTWKNGIRENTEHLSRVHRQPNRIYSIDGTHPTIPSQETAGRFFIYIPELDAVRKLT
                     INECYKIMGFKSDFIKHSNIGEQYKQIGNSVAIPVIRAIAESIKEQNLLIDEPQRQVV
                     GDLQSLFHSYGY"
     gene            22703..23623
                     /locus_tag="PLS16_00410"
     CDS             22703..23623
                     /locus_tag="PLS16_00410"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ59567.1"
                     /translation="MYWKLKYLLTFLNFIQPTRKVELSKINDERRKNIKVSQLGKVLF
                     DKKEAVLSVVDSKIEHLPIRIYKNSKQEHQKVIVYFHGGGFCFYNIESHDNICRRLCK
                     MNDCTVISVDYRLAPEHVFPSAQEDAFLVIEYIHQHARGFGINSNKIIVCGDSAGGTL
                     SACATHHFKNHPEIKIAGQILIYPWVDGRINSASIEKFSTGYLLTKEAILWFQSVYTP
                     NVEDRLNPLSAPTHHTDFSNLAPAFIATAEFDPLKDEGLAYAEKLKVAGNTVFYKDYS
                     GLIHGFANIPNIAPNGMNLFDDIKQFVEEL"
     gene            23695..24045
                     /locus_tag="PLS16_00415"
     CDS             23695..24045
                     /locus_tag="PLS16_00415"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HNJ59568.1"
                     /translation="MFLNLFRAKLHRVTVTQADLNYRGSITIDEALLDASGIVENERV
                     QIVNVNNGERFETYVIKGERGSGVICLNGPAARKVQVSDILIVIAYGFYSAEEAKVHE
                     PKTVHVDGNNQIIA"
     gene            24069..25085
                     /locus_tag="PLS16_00420"
     CDS             24069..25085
                     /locus_tag="PLS16_00420"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNJ59569.1"
                     /translation="MSSILKVLLSLALGIGAMIWFWNSMSVEDQNQTIFAFKRANYFW
                     VILAPIIGFLANFVRTQRWRLLLRPVGYNPQYTNTFYSVMTMYFFNLFVPRLGEVMRC
                     SILAKYENVPVEKSIGTMVTERVLDILCLGIVFVIVFLFLGQENYDILKANFNERTSG
                     FGNGAIFQILKFSIPALIIIGGGIFSIIYIKKNGLNKLLDLFKDKIGSLFKSIFSVKD
                     VAEKPQFIILTIGMWLLYLVMFYINYLALPETSDLPLFSALVCLLFGSFAVIITPGGI
                     GVYPIVIQMVLATFKVEPSIGLAIGMIAWCVQTVGVLLGGIISLVLLNLTNRNISDSN
                     VSTH"
     gene            25069..25527
                     /locus_tag="PLS16_00425"
     CDS             25069..25527
                     /locus_tag="PLS16_00425"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HNJ59570.1"
                     /translation="MSVLINSLEELTIIVKHHRAVKQTIVFTNGCFDLLHEGHFHLLN
                     ESKKLGEILILGINTDSSIKKLKGDTRPIQKLAERIDAIKKKSIVDYIITFDDDTPIK
                     LIEIIEPDFLVKGGDYQIENIVGNELAKKTIIIPLLEGFSTTQQINKLKR"
     gene            complement(25528..26079)
                     /locus_tag="PLS16_00430"
     CDS             complement(25528..26079)
                     /locus_tag="PLS16_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59571.1"
                     /translation="MQRRLLLLLIIFIGFSFKLDAEEKKEPYLVRTKDFKVLDFGIKQ
                     ISLGITACIYSPYKVKAKIEEVVVDVFVEDKKLGTIFEHPDYVKVPKMDTFDLPLRIE
                     VNTGATLSKFVSESARLAIGKAVKVSYKGYIKIVAMGFIPIKVKVDETEYFTRADLFK
                     KDVELPEDVIAPPLKLEHKKDKK"
     gene            complement(26080..26613)
                     /locus_tag="PLS16_00435"
     CDS             complement(26080..26613)
                     /locus_tag="PLS16_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59572.1"
                     /translation="MKKITIIALFSILLFTSCKQETKVEFKKVADIDMGNLKKPIANL
                     LAVFVFENLAEEEFDVRNLKVHLKIDGKDIGTIFNKEAKNVRGHSEFSIPVKYEYKTE
                     PVIGNNEDPENVYLIEMEGVLTLRDKAGKEFEVPLKHKSSYTYKTKKEERKEKREQRK
                     AEKALQKAEKEKLKSKS"
     gene            26816..26886
                     /locus_tag="PLS16_00440"
     tRNA            26816..26886
                     /locus_tag="PLS16_00440"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26848..26850,aa:Gln,seq:ttg)
     gene            27047..29446
                     /locus_tag="PLS16_00445"
     CDS             27047..29446
                     /locus_tag="PLS16_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59573.1"
                     /translation="MFDSLFNKLFLFHTFFFWVLNSYAVCDFQVDKTSVCADEDVTII
                     LDKPYSAYHKVIVSKGTFPLLSDATPNDFYIVPPYSANDNIVKIRFRGKNNIQDYTIQ
                     LAESAFPNPGIPCGKRVVVRVNPTPDPQITEEGNFSYCNERNPKEIKILNKSQTKNTN
                     TQYEIDWGDGSPVFVATQFDEVLHTYAVGDYVIKYKVVGNKPNTTCNISEKEYYVKIG
                     GISPRISFLPSTQTSFCAPGYYEIYPDTSVMNRNTPNTNYKFYVNDELIQTYNQNNLP
                     TILNYYFEEGSCGKSSPQCGQSSVSFYLEAYNGSCTPAILNTCVFITDSLKPYITGKD
                     VVCQTMTESFTNGNAKKDKYIAGTQCKDPIYKWSILESNGYTLKNGTSNINFDVQFDS
                     IGTYTILLSLDASSGICQDKADTTFHVSVVEKLVLDFNLPSIICMPNIEGSFVDVPIN
                     LEIKEGNPSGIKKITWTIVPDNTAPINVPNYQIISGNTTAQNMLIRFDAWRSYDIILN
                     YETICETGTIVRKVKIASKGAFDTLPIPSICAYPATINPLEYFIWEPKRVNGGDKQAQ
                     FNWTFNGGTPSTQQTKSPKVVSYNASGTYPMTLTITNVCGDTSLSPNVILFDNPQPDA
                     GKDTTLCGISNPITLIGTPSGGIWRGIGIIDSVLGIFDPKIVSAGSYTIFYTTHINSE
                     CPPTDSLIVTVLPTGISAGPDQSICRINGNQQAVLQLLSNPTFPGGSWAGAGVINSAS
                     GLFSPTNLTPGTYAVQYTYTDSVRGCKDVSSKNIVVHPAVSINMQLPNLCTQQTFVLV
                     L"
     gene            29413..31419
                     /locus_tag="PLS16_00450"
     CDS             29413..31419
                     /locus_tag="PLS16_00450"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNJ59574.1"
                     /translation="MHSTNFCFSFVGGNLIDADWDFGDGGNANIPYPNYIYTNAGDYT
                     IRLKAENSNHCSDTFNIPIKVLGEQSHEFLINNATSCNGSDFVISFPPNHDVAQTYKW
                     YYDKDSLISNQKVIPTLNFTKPLVNDTVYTVKLITKYQCSEYSQTLDLKVKANPNANI
                     FLLSDKGCAPFSTQVLNNSTGQIDSVRWFFGNGQTSTIVQPQQTPKYYNPTRADTSYT
                     ITLLTYNICASDTAKKEIVVNANEVYTAFTMSKDAGCTPLTVDFTSTVVGANSFIWNF
                     NDTDSSISFDKNPTFTFDKSGIYPVMLIANGSCGSDTIKRNVTVFGIPDANFEIINAC
                     VNQNALFQNKSTDANSFTWNFGIPNATSSNSNPTFKYTQDGAYQVQLIARNTFCADTI
                     VQDILVQSTPKAKFSIFNPEVCFSELVQFNNESTNAFNYTWHFGDNEISDATNPQHIF
                     PNPGIFNVYLVAKNTFCADSVRLNNAVRVNPNPTADFNVMIIDNDLRKPVQFTNISTN
                     AAQYVWQFGDGNSSTEKNPNHIFQYNKGYKIVLEAVSDKNCRDTIEKSININPSGTLY
                     MPNVFTPNGTGENAFFKPKGNKLKEYQIQIFSTYGQLVWESTKLADGSPVEAWDGTFK
                     GVPMPQDVYVWKIRAIFDDGTFWDGMLDESSGKKSVIGSLILIR"
     gene            complement(31421..32179)
                     /locus_tag="PLS16_00455"
     CDS             complement(31421..32179)
                     /locus_tag="PLS16_00455"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNJ59575.1"
                     /translation="MIFAAGLGTRLRPLTDRIPKALVPINGKPMLQIVIEQLKQQGIQ
                     EIIINVHYLGEQIINFIQQNNQFGIKIEISDERDLILETGGGLLKAKSFFTESNEPFL
                     VCNTDIYTNINLQHFLAKHQENKSLATLAVRNRNSSRYLLFDDEQVLFGWENAKTHTF
                     KIPRKSPRQYFIDNDGIEKLIEHPLHEFAFSGYHIIEPEIFKHCNRNGAFSMTDWYLD
                     LCPNHLIKSYIHNDDIWIDIGSQEKLAEAEKIML"
     gene            complement(32332..32652)
                     /locus_tag="PLS16_00460"
     CDS             complement(32332..32652)
                     /locus_tag="PLS16_00460"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNJ59576.1"
                     /translation="MTVPNIAERIKAIREQKNYSQTYLAQELGISQKAYSKIETGETK
                     LTVDNLFRIASALETTVYELLNTDSFNIYNNYHTHNGEGISINKQISEKTVELYERFA
                     SIKR"
     gene            33090..33245
                     /locus_tag="PLS16_00465"
     CDS             33090..33245
                     /locus_tag="PLS16_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59577.1"
                     /translation="MGVELKKILKDVVKDVDGFSKENILDILAKLVKQIFQQLRKDDY
                     LGSKSES"
     gene            33392..34945
                     /locus_tag="PLS16_00470"
     CDS             33392..34945
                     /locus_tag="PLS16_00470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HNJ59578.1"
                     /translation="MKKLKILSILLFGILAFACKKDNTDNPPNTDTNLTRFYSVEEAA
                     EDALMLTSLDSSRSIMLIAYAAFDADGIPARINSLVLGKVAENYQGNLIFDEQKRPQL
                     FYVTAKNGTKDNVLLSFSYPANDTVVYSLYYYNWSTQEDSLLQQQKINIENFNSTFTY
                     GNRLAEVAYWNKVADELETVHNNMEKVFNTEIVIKAVSDTRFNTQNLANFLETTADAN
                     RSNARIAFSAILYKYLLKLEQDRVHAQLAALSPEDFKNMKVVVHPKGLIPKAEYIYPK
                     ESKSLINYIYALFGIGSPTAEAATIPKPIGTPENPNGLPSTGTVTDIDGNAYKTIKIG
                     DQIWMAENLKTSKYNDGTEIPNVIDDEEWETLTEGAYTYYNKDANHNATYGKLYNWHA
                     VNTGKLCPQGWHIPTDAEWTQLINYLGEDEAGQKMKSTGNSTDGTGLWDKDLGYEGTN
                     ESGFSGLPGGHRYGDGSYGMGAYGGWWSSTEGGTDLAWGMLLFYDYNEVDSEEYNKNY
                     GFSCRCARD"
     gene            complement(35747..36958)
                     /locus_tag="PLS16_00475"
     CDS             complement(35747..36958)
                     /locus_tag="PLS16_00475"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNJ59579.1"
                     /translation="MQTINNINFNQERALIRVDFNVPLDKQFNITDDTRIRAAIPTIQ
                     KILNDGGSVVLMSHLGRPLKKLKEDGSIDYEKFSLKHIVNHLSNLLNTPVQFATDCIG
                     EQTLQTAQSLQAGEVLLLENTRFHKAEEKGESEFAEQLSKLGTIFVNDAFGTAHRAHA
                     STTIVANYFDREHKCFGLLMAAELENADKVLKNAEKPLTAILGGAKVSDKILIIENLL
                     NMANNIIIGGGMAYTFFKAQGGQIGSSLVEEDKLELALGILEKAKQKGVQIILPEDSI
                     IADKFDNDAQTQIANNFNIPEEWMGLDIGADAIATFSEVIQKSKTILWNGPMGVFEME
                     RFQNGTKAIALAIVEATKNGAFSLVGGGDSVAAVNKFNLANDVSFVSTGGGAMLEYFE
                     GKTLPGVAALL"
     gene            37238..38311
                     /locus_tag="PLS16_00480"
     CDS             37238..38311
                     /locus_tag="PLS16_00480"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNJ59580.1"
                     /translation="MDKFIQKLNFDFATTQRIVQLIAYIDGFKGKWNIAEKQKNRYLK
                     ELRKIATIESIGSSTRIEGATLTNKEVQELLNDIKITKLKTRDEQEVIGYYEVLELIY
                     DNYSNIRLSESYIKQLHQMLLKYSKKDERHRGIYKSLSNKVVANYPTGEQRTIFATTE
                     PALVVTEMQELVEWTNEQLEQNDIHQLIVIGSFIYDFLSIHPFQDGNGRLSRLLTTLC
                     LLQNDYTFIQYISFENHIEQHKKEYYEVLMSGQKNRRTNEERINHWLIFFLESMKTLI
                     EKLEQKYDVFKSKGGYLNNRQKLLKDFISKSEPIKVSDLAKQFPEVGLSTLKKDLQYL
                     RDEQVITMIGKGKGAIYILSEET"
     gene            complement(38385..39407)
                     /locus_tag="PLS16_00485"
     CDS             complement(38385..39407)
                     /locus_tag="PLS16_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59581.1"
                     /translation="MQRVIYLSLFVLLLSSCTCNRNKNQPKKEIEVKQSEVKVKIHRF
                     EEDWYAMNPNNIENELHQLKAKDPLIFNAYYNYVMDFPRMGSEAEQQQIITDFITKPQ
                     MRGLYDSVMKKYPNLDFLEKELTDAFVNFKSYFPEKPIPKVYTCITEFAGFPAFTYGD
                     SLLGICIDDYLGPKYIYYPNFFYDYQLYSLDKPFITIQAMHTLATNTIDAPAAQSTLL
                     DKMLVYGKILYFVETMLPKEKEANIMRYTPQQYKWCEDNIKQIWGHFLEKKILYDTKP
                     EYIKYVEEAPSTYGMPDSSPGRVGAWLGWQIIRSYMKNNSNTTLKQLIVNKDSQKILE
                     LANFKP"
     gene            complement(39467..39844)
                     /locus_tag="PLS16_00490"
     CDS             complement(39467..39844)
                     /locus_tag="PLS16_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59582.1"
                     /translation="MKNLWLLLIVVLIFACKKEPTKEKTKTELLTQKNWRRISLIDLN
                     DGQSLFEACATDDMYTFNTNGKFYYHPGTVKCGLEAAHTDNWSFSAEDSLKIGVTSYY
                     ISKLTEDTLTYLQYGWLEYKYVH"
     gene            39928..41583
                     /locus_tag="PLS16_00495"
     CDS             39928..41583
                     /locus_tag="PLS16_00495"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNJ59583.1"
                     /translation="MKIALAQQNYIIGDFEGNFEKISDAIDQAIQQNTDLILFSELAI
                     CGYPPRDFLYFNHFIEQCELIINRICAIAQDRIAVVIGAPSKNPIPDGKDLYNSAYFI
                     ENGKVKHITHKTLLPTYDVFDEYRYFESNRTFELVEYKGKKIAITICEDIWDTGETNP
                     LYTVNPVEELAKLQPDFLLNLSASPFNYKQAIKRIEIVSRNASAFNIPIFYCNNTGAQ
                     TELIFDGGSLVIDNQGRLIDELPYFRTAQKYYELDVNNTIQIINDIQNSNTKDEMALI
                     HDAIICGIKDYFGKLGLKKAILGLSGGIDSALTLYFAVKALGKQNVLSVLMPSQYSTN
                     HSVDDSLKLVENLDTSYKIIPIKEAFDVLDKILQPHFDGKPFDVTEENMQARIRAIYL
                     MALSNKHGYVLLNTTNKSEAAVGYGTLYGDMCGGLAVLADIYKTQVYDLCRWINRNDE
                     IIPKNIIEKAPSAELRPNQKDSDSLPDYEILDRVLFEYIENIKGPDEIRQILGFEEAL
                     IDRILKLVNRAEFKRHQTPPILRISPRAFGSGRRVPIVNKYCQ"
     gene            41654..42103
                     /locus_tag="PLS16_00500"
     CDS             41654..42103
                     /locus_tag="PLS16_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNJ59584.1"
                     /translation="MSLDANLNNEIKNAMLQKEEAKLRTLRAIKSAFLLAKTEKGGNG
                     QISEEQELKVLQKLFNQRKESFEIFTKENRAELAAKEKEEMDIIGAYLPAQLSDEELK
                     STIQQIIQTLGVTSIKEMGKVMGAASKQLQGKAEGGRISSVVKELLE"
     gene            complement(42126..43355)
                     /locus_tag="PLS16_00505"
     CDS             complement(42126..43355)
                     /locus_tag="PLS16_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNJ59585.1"
                     /translation="MSIERIIAQRIAHGEEQSFSRFIIRIAIIAITLSVTIMICSICM
                     VGGFTREIKEKIFGFWGEIHIHNLDNKSSFEAEPIDVSTININKIKSLSGVKIVSPYI
                     TKAGILKSKTEIEGIAVKGIDQHTDWTFINDYIVDGKAINYNDTAFSKDVLISKVLAN
                     KLNLKVDDNIIVYFLKKQTTQPIGRKVHISGIYKTGLYEYDEKFVLCDLRLLQQINMW
                     QPNEVTGLEVRLKEMGKMDEIKDKIYYTIIDNTKYAETIKEVYPSIFEWLNLQKTNEY
                     IILLIMAVVALLNMISALIILILDRTQMIGILKALGVSTRKIEKIFIYQASYIIFYGL
                     LFGNSLAIILCLIQKYTGIIKLNEENYYLSEVPVYFDIPKILVVNILTFVICFLVLYI
                     PSRIIGKISPIKAIRFD"
     gene            complement(43385..45406)
                     /locus_tag="PLS16_00510"
     CDS             complement(43385..45406)
                     /locus_tag="PLS16_00510"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0033543 - fatty acid beta-oxidation,
                     unsaturated, even number, reductase/isomerase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HNJ59586.1"
                     /translation="MANQYPILLSELDLGFTKLKNRIIMGSMHTNLEESKDGFQRLAT
                     FYAERAKGGVGLIVTGGIAPNWEGWLKPFAERMTNRSHVKKHKIVTDAVHAAGGKIAM
                     QILHAGRYAYHPLSVAPSRIQAPINPFKPRALTTWGVKRTINDFADSAALAKEAGYDG
                     VEIMGSEGYLINQFLVKHTNQRTDEFGGSYENRMRFPVEIVRKVREKVGPNFIIIYRL
                     SMLDLIDDGSSWEEIVQLAKAIEKAGATIINTGIGWHEARVPTIATSVPRAAFSWVTQ
                     KLKSEVKIPLCTSNRINMPSVAEKVLADGCADMVSMARPMLADENWVQKAIDGKEEEI
                     NTCIACNQACLDHTFSNKLSSCLVNPRACTETELNYTKTNNKKKIAVVGAGPAGLTFA
                     KVAAERGHQVTLFDSEKEIGGQFNMAKTIPGKEEFYETIRYYDVHLKKYKVDVQLGKR
                     VNADDLKDFDEIAIATGITPRHINLEGADHPKVLSYIDVLKNKAKVGKRVAVIGAGGI
                     GFDVSEYLTHQGESLTLNREEWLKEWGVDRTNETRGGIIPQVIAPPAREVVMFQRTAG
                     KVGAKLGKTTGWIHRATLKMKKVKMIAGVEYTKIDEQGLHFIDKNKMAQVFECDNVVV
                     CAGQISLRELEQPLLALGKTVHVIGGAYEAGELDAKRAINQAAHVAAGI"
     gene            complement(45477..46718)
                     /locus_tag="PLS16_00515"
     CDS             complement(45477..46718)
                     /locus_tag="PLS16_00515"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNJ59587.1"
                     /translation="MLFLLFIIIIVSILSGIEAIFQTTNRLKIELSYQKGDYTGIILN
                     DYIKNPSKYDITITLGIYLLLVIFSIVSFFHFLPLIDILGVPFALAGFILILINTSIF
                     LLLGVYIPKSIFKLFSDNLLPIFIYPFNVLHWILQPISVIVQALIKFTLSLFGIKNDT
                     ATIFYAGIDTDSFIKEHNIEEDNDDDNNVDQEFLENVLDLKNLKARDCMVPRREISAI
                     SKTGTIDALKEIIVKTNHSRILVYDESIDKIIGYIHHFDLHKKPKDILSILIPIKIIP
                     ETIHIKKLLNDFIKDQKSIAWVVDEYGGTAGVITLEDILEEIFGEIDDEYDKDEFIEN
                     QLSANEYILSGRLEIDYINKEFDLNLPEGDYDTISGFIIEHYETIPKQNEIIEIGNYI
                     FKILNVSETKIETVKLEVKQK"
     gene            complement(46760..47302)
                     /gene="lptC"
                     /locus_tag="PLS16_00520"
     CDS             complement(46760..47302)
                     /gene="lptC"
                     /locus_tag="PLS16_00520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNJ59588.1"
                     /translation="MRLIYFFILLILVSCQENDMAKIDALINEKEANIEVADSVKLTY
                     KEGASLKAEIYGKSVSRYLKGDNKLIFEKGIEVRFYDAGQLSSVLSADSASYDDNLRT
                     ILIKGNVDMYNYKHEELSTDELLWDMNTKMIFAKNHIKIKTPHDIVRGSGMIAKEDFS
                     QYSIKRVFGTVAYDDEERFR"
     gene            47424..48164
                     /locus_tag="PLS16_00525"
     CDS             47424..48164
                     /locus_tag="PLS16_00525"
                     /inference="COORDINATES: protein motif:HMM:NF024599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCMD domain-containing protein"
                     /protein_id="HNJ59589.1"
                     /translation="MKRLYYLGLVLITTIAVFTACSKIKIEKPKLKNNLMTCDSIRKG
                     GVFVEIPNLDFEDWTPSSSKRYEEPSLTCFWATPSKANDIISAIPITVSKVTGDDAHS
                     GKYGCKIQSQAWGALFTSGTVASGKFSPDFKNPLQSIQFGQPFNKKIKEVRGWYKFQS
                     VKQDSCSFYCYQLKKVGSQVDTINFSRIVTNVSQPNWTEFVLTPVYRKDDEPNVLVLY
                     FASSEAGSELKGQVGNTLIIDDISITYY"
     gene            48175..48999
                     /locus_tag="PLS16_00530"
     CDS             48175..48999
                     /locus_tag="PLS16_00530"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNJ59590.1"
                     /translation="MKNIFCFVILTFGFFIDGFSDTIVTKKPFYEPKVKMSKMDYGIN
                     VGMTLAFPLGVLVEGAKGMPFPGPSIGINTRYYFSNKFSIQAGINYYWNQTRFETPYA
                     NFSYVGAIDISMPDGSTVQKFDTVNIYYAVVKDGLFSNKYLGFPIQGNLHINKVWSLN
                     AGAYLAILLKGGMTGMATDVVLGDGSSSDFQVSDDVPFDQGDQFAKFDYGMNFGTNAQ
                     LPNGFNFDLKVNMGIASMFKRSFTAPPGIYRNVFFQGTLGYRLGAKERFKPNLPKI"
     gene            complement(49054..49527)
                     /gene="greA"
                     /locus_tag="PLS16_00535"
     CDS             complement(49054..49527)
                     /gene="greA"
                     /locus_tag="PLS16_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNJ59591.1"
                     /translation="MSEIVYVTEESLEKMRQELQHLVAIERPNASKAIAEAREKGDLS
                     ENAEYDAAKEAQGHLESKIRKLEAAIGNARVVDTSTIDTSKASVLCKVKVKNLKIKKE
                     FTYQLVSENEADVKQMKISVKSPIGAALLGKSKGEKVMVQIPAGAMELEILDISI"
     gene            complement(49662..49745)
                     /locus_tag="PLS16_00540"
     tRNA            complement(49662..49745)
                     /locus_tag="PLS16_00540"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49709..49711),aa:Leu,seq:gag)
     gene            complement(49933..50796)
                     /locus_tag="PLS16_00545"
     CDS             complement(49933..50796)
                     /locus_tag="PLS16_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ59592.1"
                     /translation="MSIYKNKETKIAIQELYYEKLNSLKIEYQEIDVNTKYGRTRVIK
                     TGKSNGTKIIVFHGMNTGAPVALEAIKELTDSYNIFAIDTIGQATQSEETRLNIKDNS
                     FALWADEVLEKLQIKTANFIGVSYGGFILQKLISHKPERVSKCIFVVPAGLVDGAFFP
                     SLTKVTFPLIKFLLTKNDNHLHNFLSKFINEDDNFMFRLQKAMLLGVKMDYRRPPILK
                     IKDVMHFEKPVYLMTVDNDIFFPGNESIKKAQNIFKNLQDIYILENNKHMPNKKQYPE
                     IQQKIREWISK"
     gene            50868..51461
                     /locus_tag="PLS16_00550"
     CDS             50868..51461
                     /locus_tag="PLS16_00550"
                     /inference="COORDINATES: protein motif:HMM:NF025338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNJ59593.1"
                     /translation="MIKNRIKDKGRELFNEFGFRNITLRDIAISINKSYGNITYHFPT
                     KEHLLQAIFSDMNDRLIALQSNLPKDINYLEYFLLLPYYSFDISVDYLFFFKDFVEIK
                     RAYPKFYEIVEKTNNFRKEKWKDLFILLQQQSYLENTLSEQDLFYIMELSTGIRIFYF
                     MENDLANLNKQIFVQKVNKLLYPYLTKKGKSIYQMNK"
     gene            51692..52141
                     /locus_tag="PLS16_00555"
     CDS             51692..52141
                     /locus_tag="PLS16_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59594.1"
                     /translation="MRNVLFIIFFIGTITLFANPLLIGDWNGFIVQKYETDNPIFLPC
                     TLNISSVSSSNQFAGTIEISFKHANGKVYKSKATVSGIYDTKTYKISIVDEQFVYQDL
                     LPEKAKWCLGITKGGFYRSKTAKQYLIKGLYSTKCSADVGLLVVYKK"
     gene            52152..53078
                     /locus_tag="PLS16_00560"
     CDS             52152..53078
                     /locus_tag="PLS16_00560"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ59595.1"
                     /translation="MSKWFIKALLQRSFSISKNPEKINRLFQRYLTGKDKLNEEKFGY
                     KILHASDHIAYFKKHSNKSLADVNCLELGTGQFPIVPIFYFLNGMNQITSIDVVSFIT
                     KDRIEDTLNFFIEYRKKFPEKFSEILEERWNILADTLANFAQQDDMHKILKNFGIEIY
                     IADARKTKYPESTFDFISSNNTFEHIYPQILEGILKEFQRILKPEGIMSHFIDMTDHF
                     AHFDNKINVYNFLKFSDKFWHRIDNEILPQSRLRFKDYLAIYQSLNIPIIDQKNWDYD
                     VSLIDSIQLNKKYQNYTKEELAIVHVYVVSSK"
     gene            complement(53040..54434)
                     /locus_tag="PLS16_00565"
     CDS             complement(53040..54434)
                     /locus_tag="PLS16_00565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNJ59596.1"
                     /translation="MKQLQKNKTLYFLLILVFVCRLLLLFIFKPWLPEIQDKVVLLYD
                     AVGYHKLALILLETFRIKNEILRTPIYPAFIAVFYALFGVKPFIVLFAQIFISVATGY
                     FFYKIIAQLSNRMIANIALFIFAIEPTLILYTTFLYSEVLFMFFTMFSLYLIIGGLKQ
                     NKILYFILAGISLGLATLTRPAGQYLIIVYVLLVIFYTLKNNSSKLKFAVLLTVFYLL
                     SIFPWAMRNYIYYGRFKLSNTMDHNALILSSFYTEFQNSKLPRDTIIQHFLYQLKAAA
                     PTNLKDSMPSNFAELNRRQSFENTDIFAKVAKNYLLSHKKEFILAQIQGAINLHVNMG
                     TEQYMLRLHQETKRWDIKDKVGLGLFAAAKKFFATKTTTEIALGIFILSLLVIVYGYT
                     LVGIGYTIKNKDFYILIFCILNIGYYVSLSSIYPTPRFRNPFMPFYIFLAAYGIYNLH
                     QKLFGRNNINVNNR"
     gene            complement(54506..55696)
                     /locus_tag="PLS16_00570"
     CDS             complement(54506..55696)
                     /locus_tag="PLS16_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNJ59597.1"
                     /translation="MTLVKHNESEVSSKKKIIVLGAGPNRIGQGIEFDYCCVHGLLAI
                     KEVGYEAIMVNCNPETVSTDFDIADKLYFEPVYWEHLWEIIEHEKPEGVIVQLGGQTA
                     LKLSKKLHDKGIKIIGTSFEDMDTAEDRGRFSDLLKEIGIPYPDYGTAINADEALEVA
                     NRVGYPVLVRPSYVLGGQRMRIVINDEECETAVINLLRDIPDNVILIDHFLDRAKEAE
                     IDAIFDGEQMQILGVMEHIEPAGIHSGDSNSVLPPYSLGPMIVETMIEYTEKICKALN
                     IKGLINIQFAIKGDKVYVIEANPRASRTTPFICKAYNVSFLNVATKIMLGTHKLKDFK
                     LEPKLEGFAIKEPVFSFDKFPNVDKELGPEMKSTGESIYFIKSLSDPYFRQLYKEKSM
                     FLSK"
     gene            complement(55806..56288)
                     /locus_tag="PLS16_00575"
     CDS             complement(55806..56288)
                     /locus_tag="PLS16_00575"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNJ59598.1"
                     /translation="MTEKLIPIKYVVAETITELDEQEQKLIEQAKLAKEGSHAPYSNF
                     NVGAALLMANGKIVQGSNQENAAYPIGLCAERTAISASVSLFPTEKVKTIAIVASSQN
                     MEVPFPVAPCGICRQTLLETESKQRSNIRILLHGNNGTTFIFNSIKELLPLHFDAEYL
                     "
     gene            complement(56285..56851)
                     /locus_tag="PLS16_00580"
     CDS             complement(56285..56851)
                     /locus_tag="PLS16_00580"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNJ59599.1"
                     /translation="MIDETRNPFQTIDETIVYDTKWISVKHANIITPSQTKGIYGTVH
                     FKNYAIGIIPIDKDGYTYLVGQYRYALNTYSWEIPEGGGPLDMDILESAKRELQEEVG
                     FYANQWTKIAELNTSNSVTDEIGIIFVAQDLIPTNTNYDETEEIQIKKLPITEAIDWA
                     ISGKIKDAIALVGLLKLKVLIEKSEIKL"
     gene            complement(56882..57559)
                     /locus_tag="PLS16_00585"
     CDS             complement(56882..57559)
                     /locus_tag="PLS16_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNJ59600.1"
                     /translation="MKKIVVLSGAGISAESGIKTFRDSDGLWENHRIEDVATFEAWTR
                     NKELVLAFYNERRKQLLECQPNIAHELLAKLESKYDVEIITQNVDDLHERAGSSKVIH
                     LHGELLKVRSTKDERLVYPWKKDLNIGDNCEKGDQLRPHIVWFGEQVPMIEKASEICK
                     RADIFIVIGTSLVVYPAAGLIHYTDFTVPKYVIDPNRPSVSYTENINYITEKASIGMQ
                     LLFDELM"
     gene            complement(57616..>58203)
                     /locus_tag="PLS16_00590"
     CDS             complement(57616..>58203)
                     /locus_tag="PLS16_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNJ59601.1"
                     /translation="IEMNFLPDVYVECPRCLGKRYNRETLEVKYKGKSIYDVLEMPIV
                     EAVSFFEHIPQIYRKLKTLDEVGLGYIRLGQSSTTLSGGEAQRVKLSEELSKRDTGNT
                     FYILDEPTTGLHFKDIKILLEVLQTIVNKGNTVVVIEHNIDVLKVTDYIIDMGPEGGS
                     GGGEIIAEGTPEAIAQNKKSITGKYLKEEFSVNNN"
BASE COUNT        19727 a         8956 c         8815 g        20705 t
ORIGIN      
        1 ttcgtggtct tttatgcttc tgacttacat tttgagcata gcctaagagg cggattatta
       61 gcataaaagc tcaatcgaaa gactaaacta caaatatagc aaaaatgttg aaacgaatca
      121 tttcgcccgc ttccttgcaa aattgcccgt tgtacgacgt cgcaattatc ggtttaaaaa
      181 ttcttttgcg gttaatattg gtaaattcac attcttgaaa tcttttttat ttcgagtaat
      241 aataatatcc atatcatttt caatcgcagt gtaatattga attgcatctt caaaatcttt
      301 taaatcagaa gttaatgaca aatcaataat tttgtcatca acagcaagaa cttcaaccaa
      361 aactttgaat tttgctaata cctttcttgc ttccattttc aaatgtttgt ataacaaaaa
      421 atgaacattt gctaaagtta aagccgaaac aaacaatttt acattctttt tatctgaaag
      481 tgtgaaaagt tcttgagctt cttgataaaa ttcctcacgt ttttctaaca aatccattat
      541 gatatttgcg tcaacaagaa ctttctccat tatgaatatt ttttgctaag atattcgtaa
      601 taatcatcat tatttttttc ctcggtttgt gttggaatta ttccagttaa actttcgaca
      661 agtggactta cttttatttc tttatcctct tttttgatta tagaatttag gtagttttca
      721 attaattttg aaagactaac cctattttct tttgcataat cttttgcact ttcaattatg
      781 cttttgttta tatttaaagt tagttttgta ttcattacat tggtttacgt gtaaaattaa
      841 taatttttta cgtaaaacac aaattttgtt ttgatgagag tctcaatgcg atgttgaaca
      901 atggttttac gacttgacgt aggcaaggca ctttaattct gctgttgatg cgaataccta
      961 agtttaaata tagcacattc catattacag catttactgc ataaagtctt atttataaag
     1021 ccctttggct tgcatttcat ttaatgattg ctcaaaatta tttttaacta aaggttgtgt
     1081 ttttacccaa tcgacatatt ttttgatacc ttcttgaaaa ctgatttttg gctcaaaatt
     1141 taaactgttt tttagcaaac taatatcagc aaaattatgt cgaatatctc ctttcctaaa
     1201 tttttgtgta atctgaatat caacttcaat ttcgtagttt ttaatcagtt cttgagccac
     1261 ttcaataaca cttgttgcct ttccagttcc aacatttatc gccttattgt tggcttcatt
     1321 attctcaata cttaataaag ttgcttgaac aacatcatct atatatacaa aatcacgact
     1381 ttctttgcca tcttcaaaaa ttaaaatagg ctgcttttct ctaattctat tggagaaaac
     1441 cgccaaaatt ccagtatatg gattagataa ggattgacca acgccataca cattttgata
     1501 ccgcagagca acataaggaa tattaagtgc agaacaagta atctcaatcg ctttttcttg
     1561 ttcgtatttg tttaaaccat aaatagaatt aggttgtaca aaactcattt cgtctgtggc
     1621 acatagctca acttcttggt tggtaattgg acatttacat tcaaaatctc catttaataa
     1681 atcttgttca tttcggtgtt ttgggtaaac aaattgattg atagtaggtg agaaatattt
     1741 tccctcgcca tatattgctc tcgatgaagc gacaacaact ttttctactt gatgctcaaa
     1801 attcattaaa gcttccaata aattacttgt acccatcaca ttcgtttggt tgtatctttt
     1861 aatttgatac atactttgac ctgttccagt ttctgcagca aaatgcacta taatttgttg
     1921 attttttaag gcatctttcc aaattgttgg atggtcattt gcaacatcat gatgaatgaa
     1981 acgaacttta ttttttatag cttgataaag tggagatgtc tgttcaggat ttgctccatg
     2041 aatttgagga tgtaaattat ctaaaatcga aatttgatgt cctttctcta tcaattttaa
     2101 agctagatta gagccaataa atccagctcc gccaataatt agaatttgct tgctcatttg
     2161 ttttgttgat tggtaaaagt acaatagttt agtgtaaatt tcaattttag caactttttt
     2221 cgtatcattg agaagaacgc ttttttatga aaaagataga tttaattatt ccaggctcaa
     2281 aagacaaacc aattacttta gatattcaat atcaggaatc agataataat ataaaacctg
     2341 ttgttttatt tatgcatggt tttaaagggt ttaaagattg gggacacttt aatttgattg
     2401 ccaaaacttt tgttgaaaat ggttttgtat ttgtaaagtt gaatacatca cacaatggaa
     2461 caacacctga gcatttagaa gattttatag atttggaagc atttgctaat aataatttct
     2521 ctatagaatc tgatgatatt gatatggtaa ttcaatttat tgaaaccaat attcatcatt
     2581 attttggcga taaaaatcaa ttgcatatta ttggacatag tcgaggtgga ggtttagttt
     2641 tattggctgg acatcgaaat ccaagtatta agaaaatcgt tacttgggca actgtccaaa
     2701 atcttaaata ttttttcgat tatatggata taaaaaagtg ggctgctgat ggagttgcct
     2761 atattccaaa tagcagaact aaacaagata tgccatttta ttatcagatg tatgaaaatt
     2821 acttgttcaa tcaggaaaaa ttagatgttg aaaaagtggt ttctgaaaca gaaaaaccaa
     2881 tactatttat tcatggctct ggcgatattg gtgtgccaca aaaatattct gaatatttac
     2941 atcatcaaaa ccctaattca tctctagaaa ttattgagca tgcagaccat acgtttggcg
     3001 gcaagcatcc ttggatagct gatgaactgc caggacattc taaaatatta gttcaaaaaa
     3061 caatagattt tcttagaaag taacagccta ataaaaattt attttgtttc aaaaatagcc
     3121 actggtacaa taacttcttc taaggaaatg ccaccgtgtt gaaaggtgtt tctaaagaaa
     3181 tttacaaaat aattatagtt atttggataa cagaaataat agtcttcctt agcaaaaata
     3241 taggaagaac taacattgga tttaggcaag aatgcttcat ctggattttt aacctcaaaa
     3301 acttcttttg gattaaagtt taggtttttt ccagttttgt atctcaagtt tgttgtggta
     3361 tttttatcgc caacaacttt tgatggtgtt tttacacgtg ttgtaccgtg atcggaagtg
     3421 ataacgattc ttgctttttt gccttgcaat ttttccaatg cactcaacaa aggcgaatgt
     3481 tcaaaccaag acaatgtcaa agaacgataa gccgcttcat cgtttgcaat ttcttttaaa
     3541 acttccattt ctgtacgcac atgcgataac atatctacga agttatagac aatggcattg
     3601 aattgattgt gcatcatatt caaaatatta tcttctaatt ctttgccgtc tttatgattg
     3661 gtaatcttgg tataagagct tttgatgttt aagccattgc gtttcaattg ttcttccaaa
     3721 aagaaagctt catgtaaatt ttttccttca ttctcatcat caaaaaacca tttatctgga
     3781 tattttttgt tgatttctga aggcattaag ccagaaaata tagcattgcg actatattgt
     3841 gtagtcgttg gtaatatgct atagaaaaaa tcttcactta cttttttgaa gcgttctttt
     3901 aaaatgggtt caagcatttt ataatgatca taacgtaaat tatcaatcaa gataaaaaaa
     3961 gttggaacgt cgttatctag tataggaaga acttttttct ttattaaagt atgagattgt
     4021 gcaggcgttc cagcatcagg cttttgtaac cattttgtat agtttttttg tatgtatttg
     4081 taaaattcct tattggcttc tactttttgc atttcaaaaa catctaacat atcggtattt
     4141 gacgatttgt ctaattctaa atcccaataa attaattttt tatataattc agcccatgct
     4201 tcagcatcta aatcatcgtt taattccatc ataatttgct gaaattgttg tcgataactt
     4261 accgaagttt tttcagaaac caaacgctta ttgtctataa tttttttaat tgacaataaa
     4321 acttgattcg gtcgaactgg tttgattaag taatctgcaa tttgagaacc gattgcctct
     4381 tccataatat tttcctcttc gcttttggta atcatcacaa caggtaaatc attatgcaag
     4441 aattttatct tggataaggt ttctaaacca cttataccag gcatattttc atctaaaaat
     4501 accaaatcaa aatcttcctc attgcatttg tcaatcgcat catagccatt gctcacagca
     4561 cttattttgt agcctttttg ttctaaaaat aaaatttgag gttttaataa atcgatttca
     4621 tcatctgccc aaagtatctt cgtatccatt tgtcgttttt atggtttaaa attattttga
     4681 aagttaatac aattaaaatt agttaggaat atcaagttac taaaaaatgt gaatttgtat
     4741 gattaatttt acaaagatga ataagagaaa gatttttaat gaccctgttt atggatttat
     4801 cagtattcct tttgatacag cttttgatgt aattcaacac cctatttttc aacgactaag
     4861 acgtattcaa caattaggtt tcagtagttt agtttatcct ggagcaacac ataccagatt
     4921 tcatcattca ttaggagcaa tgcatttgat gactagagca ttagatactt tgaaaaaaaa
     4981 agatattcaa attgatacag aagagataga ggccactgtt ttagctattt tgatgcacga
     5041 tttaggccat ggaccttatt ctcatgcctt ggaacatacg ttggttgatg gtgttgatca
     5101 tgaagtaatc tctaaattat tgatgttgga attggaaaaa gaatttggag attcaatcac
     5161 aaaagcaatt caaatatttg aagataaaca ccctaaaaaa ttcttacatc aattggttag
     5221 tggtcagttg gatatggata gaatggatta tttgaatcgt gattcttttt atactggtgt
     5281 aagtgaaggt gtgattggct atgatagaat tatcaatatg ttgaatgttg agcaggataa
     5341 tattgtggtt gaggaaaaag gtatttattc tatcgaaaaa tttttagttg ctcggaggct
     5401 gatgtactgg caggtatatt tacacaaaac ggttttgtgt gctgaaatac ttttaaaaag
     5461 catctttaat tatattagaa attttggcat aaaattagac tttgatacac cgctcaatct
     5521 ttttattgaa aataaatttg acaaacaagc gtcaatagaa tggctgtcag atttctgcca
     5581 attagatgat acggatattg tttttgcttt aaaacacctt tctaaaaaga aagaccctat
     5641 tttacaaaaa cttatagatg cattgttgta tagacaatta tttcatatcg aatttgctga
     5701 tgtgcagcgt ataaatatta tttatgaaag attaaaactt aaatacagtg cagaagaaat
     5761 ggactactta gtctttcgtg gaagtacatc taataatatg tacaatactg aaaaagataa
     5821 tatttttata caaatgaaaa gtggtaaaac cattgatgta gctagtattt cggagcaatg
     5881 gaatttagat aaaatttcta atccagttgt gaagtactat attatttatc caaaaaatta
     5941 aattaattat tacaaaaata aatttcatga cattagaaat tattcaacaa atagaagctg
     6001 gtacagcaaa tttcaaagat gtaattgctt atattgattc aaagtatagc cacaccccaa
     6061 cggcattcaa aaatggtaat caagaaaatg ctgccgatca aaatcaaggg agtgctaaag
     6121 tatttgcttt tggcaagtta aatgaattgt cagaggcgga tacgttaaaa ttatttgtgg
     6181 aacattatca gtcagtttta gctacaccct caggtaccga ccatcaaaat attagacagt
     6241 ttatgcaaaa tggctggagt ggaatttctt tttcaggagc ggcattggtg ctaaagtaat
     6301 tatataaact ataaagaatt tgttattggg tagtattgtg atttgagaat atttctggat
     6361 ggtaaaaatt ataaaaaaat tgtgtggaac aactaaattt agtaatttag tagaaaaaag
     6421 aaatggcaaa aagaacaaat catgttgtgc catcttcatc aggatggtca gtaaaaaaat
     6481 caggttcatc aagagcttca aaagtctttg ataataaaga aatggcgatt gattacgctc
     6541 gtaaaattag tataacagaa agaactgaat tgtacattca taaaaagaac ggtacaattc
     6601 aagacaaaag atcttatgga aactatccta atgcaccaaa gaacaagaat taatgaaata
     6661 tgaaaattct gtatttatta actgtccatt tgacgatagt tttataaatg atttattaaa
     6721 gcctacatta tttttcttga tatataatgg ttataatcca cgcttatcat tagaaatctc
     6781 agattcaggt caagtaagac ttgaaaaaat tactgaaata attaaaaatt gcaaatatag
     6841 tattcatgat ttatctatag taaaggctaa aaaagttaat gaatatgcta gaatgaatat
     6901 gccttttgaa ttaggtattg attatggttt gagaaattgt gttgataaat aatttagtga
     6961 taagcaattt ttaattttag aagcaaatag atatgattat caaaaagcat tatctgatat
     7021 taatgggttt gatattaaag tccatggaaa taatactgaa aaaattattg aatgtttata
     7081 tacatgggga agtgagaccc taaaaataaa aaaacaaaat cctcctttaa aaacatttta
     7141 tgattttatc gactttaatg ctaatctctt tgaagagaaa ttagcggaac taggaagtga
     7201 aaaattggca aaaaattata ttgaaaaaat aagtatagca gaatatattg aagaaattag
     7261 agaaagaata taacctcttt aagtacaaag aatgtaattg tgaaaaagat gcttgggaag
     7321 aaatagtcgt tcaacaagat gaccattttg gaggcaagac agttatttac tttcattgtt
     7381 cagattgtgg tattgatttt agagttgagg atttttatac tcatgaagaa ttacacccaa
     7441 agataataga atagtcatta ctattgagtt tagtatatta ttaaacaagg aaatttcaaa
     7501 ataggtagaa ttaccatgca gcgaaaaggc ggagataatg gaagagaaac tgcaaaaatg
     7561 ttgcaattca aaattaatcc agcagaacta tttgaagtgg aataatttta ttctaaaaaa
     7621 tgtttttaca attttatatt cttatagtac atttttaata tgctttctgt aagcgattga
     7681 tagatgtttt ttgtcaaaat atctttatcc agcatcgata aatgtttgtc aataatatta
     7741 aaatccttac gctttgctgg accagtttgt gcatcaattg caggaacaac atcaatctta
     7801 tttatcgttt gatgaatcaa tggtttgagt aaattaaaat ctaattgatg gtctttgcaa
     7861 tattgttgag caattgcaaa caaatgattg acaaaattat tgactaaaac cgctgacaaa
     7921 tgcaaatgtt gcctatcctc gtcattgatt acaaaagttc tttgtgtaat cttagatgcc
     7981 aaattaatta attctgcttc aaagaaattg tttttagtgg taatgcctat tggaatttca
     8041 gaaaaatcaa cagcatttag ttttgagaat gtttgaagag gatagaaaat accaacagct
     8101 tcaaatttat tttcaaagtc tgaaaatact tctaaaccaa ctgttcctga agtatgaacg
     8161 atgcttttaa ttttcttttt tctggcaatt ctttttgata ttttacctat taattcatct
     8221 tttgtacaac agaaaataat atctacagtt tcatcaagat tatcaatatg gtcgatgggt
     8281 tctgcattta cttttgttgc taaaacagta gcattttcca tcaatcgact acaaacttgt
     8341 ttgattttta ttcctttgtg aaataaggct tgagccaaat gcgtagcaac atttccactg
     8401 ccaataaaag tggcagtttt aagcggctgc atattttctt ttattatcac ttagtcgctt
     8461 ttgcatacta ataaaaattc cagaaaccat cactaaaata cccaaccaca atacattgat
     8521 aaatggaaac ttaatcgctt tcataattat atatttatgt aaagtttctt caacagtaaa
     8581 ttcaaattta ttgctttcag gtataattct agttactgca atttttgttt tatggtcatg
     8641 tgcttccgcc tcaatagatg tagccatcat atctctaata aaataaacag gttctatggt
     8701 gtattgtgta tccaaatcaa catatcgcat ttttacacca actgccacat catttggttt
     8761 tttctcaatt ccttttactt cagcatgtgt attaatgcct tctataattg caaatgctct
     8821 cgaaagcatt agcgtatcgc caactcctaa agtatgtttt tcttcaacaa tactatctac
     8881 tggagtacca tcttctttca aaggagccat agttatatgt gtatataaat cagattctaa
     8941 agtttttcta gtagaaggaa ctggattatt acccattttt tgatttagta aaatgtttgg
     9001 atgtaaagta aatcttttta caatttcatt tttcttattc atttgctcat aaagtacatt
     9061 gaaatagatg tttttatcgt ggatagaatc tcctaaataa gttactttat atttgcccat
     9121 tacttgtggt tcatgctgat acagcaaaat attttcagca ctttcatcgg cagtaaatcc
     9181 tgaaccataa ttgataccat atctattaat agaaacgact tcttgttcgc cttgagaaac
     9241 aacaattccc accatcatta agccaaaccc aatatgtgaa acataaccac cactgacttt
     9301 aacttttcct ttcatcacat tttttatata gttgatatta gaaaaaattg taaaccaacc
     9361 agctaccaac atcaaaatat aatcgattcg attgatttta taataagcta caattccgat
     9421 agtaactata attccaccag ctaaactcca aataaaattt ttgaaaaatc cttttaaatt
     9481 agttttcgtg aattttaaaa attgtaccat tgctgtgcca aaagctgcaa acattgctaa
     9541 ccaaatttgt acatgattat aatgcgattt aatatcagaa ggtggtgcta atttattttt
     9601 gataaatggc aatttattaa atactggaaa agaagttaat ccagtaatat gaatagcaga
     9661 aagcgtaaat aataaagcac cgataaataa ccaaaattct cttgaacttg ttgcctcttc
     9721 ggtttccttg aaagggattt gttttttgaa acttctataa aaatataaac ctaatgacgg
     9781 aattaaaaag accagtattg ttgatattaa caagccactc atacctaagt cagtaaatga
     9841 atgtactgaa gcatcaccca gtacaccact tttggtcata aatgaagaat ataatactaa
     9901 taaatacgcc attaggaaaa atgcgtaagt gatacttaat gagtgtcctg tgtgtttgta
     9961 aacgatgacc gtgtgtaaag ctgctgctaa tattaaccaa ggcactaaag atgcgttttc
    10021 aactgggtcc caagcccaaa aaccaccaaa attcaatgct tcataagccc aagctccacc
    10081 catcaaaatt ccagcaccta atatcattaa agcaattaat gtccaaggca aaacagcctc
    10141 agcaaattga gtatattctt ttttccataa agaagcaaat gcaaacgcag caggcacagt
    10201 agttaatgca aaacctaaaa ataaagttgg aggatgtatt accatccaat agttttgcaa
    10261 agaaggattt aagccagtgc catctccagg aatataattt ggattcatct taaaaattgg
    10321 aatatccata gtgtctttta ataaggcaaa tgggctactt cctaatttat aatcaaaaat
    10381 ataaatacct aaaagcattg cacagagcat aacttgagaa agagaaacaa aggtcataac
    10441 acctgtttca taagactttg ctctgaaaat tagcacacaa ccaacaattg cagtccagaa
    10501 aatccaaagc attgtgctgc cttcctgacc agaccacata gcagaaaaaa tataaaatac
    10561 aggcaaatcc tgagaagaat gttgccatat atatttatac tgaaagctat gggtaaataa
    10621 taaaataaat aaaatagaaa atataccgat ggctgaaatc gtatgaacga taaaagcttt
    10681 tcgtcctaat ccaatccaag atgcagtatt gctattgtta ctgtattgtg ttgctataaa
    10741 atacgaaata gtagaaacta taaccgatat aaatgtaata atgataaaga gatgtcctat
    10801 ttttccgaat attaaatcag agcctgtact ataaatgtcc atgtattagc tatttgtttt
    10861 ttcttgatat ttacttgggc atttcattag aatatcatca gcaatgaaag tgtcatcatg
    10921 catttttccg ataacaacaa ttttttcact tctttcaata tctcgaggtt tggctttttt
    10981 tacaattact ttcttctcaa cacctttggc atcaacaatg tacatagaga aaatattagg
    11041 atttttggta ggttcataag taataccttt tactttattt aattcgccaa cgacgttaat
    11101 ttctttgcct tttttggcat aattagattc aaatgtttcg tatctactga aatcgcctag
    11161 taaagtaacc agtactccaa tcataactgc aaccaatact aaagcgataa tgtgcgtttt
    11221 tttcattatt gcaaatttta aacttcaaag ttactaggaa taacaataca acagtattaa
    11281 ttgttgttat agaatagtaa aatgtattgt cttattatta aatcacaaaa aggactaaaa
    11341 tatcctattt tagattaaaa ctattctgta cttttatttt atggagaaga agcatttatt
    11401 atttatttat cattcacaaa gtggacatac ggctcaatta atagaaaaat gtaagcaagg
    11461 agcatcaaaa gaagaagttg aaatccgaca tttgaaagcg tttgatacaa cattagaaga
    11521 tgtgctttgg gctgatggta ttgtaattgg aacacctgaa tattttggaa caatgagtgg
    11581 tgctttaaaa gatttttttg atagaactta tgatgatgcc aagaaagcgg agattaataa
    11641 accttatgct ttaattattt cagcaggaaa tgatggaaca ggagcggaac gccaagtaca
    11701 aagtttgaca actagttaca cgatgaaacg tgttttggat actataattg taaatgaaac
    11761 agaatttgat tcgaaactta ttgatgtgga ggaactgggt cagacatttg cagcaggttt
    11821 ggctctgggt attttttagt aatataattg tcaatacatt aaaaggaata atagttacag
    11881 cactagtaag ttatacgatt ttacgactta ggcttcataa cgactaaatt actggtggct
    11941 ttagcaatta ccatattttt agaattgtat aatttagctt cagtatgtac aacagtattt
    12001 ccagctttaa taacatttgc ttctccacga atgacttcgc caacttttgc aggacttaaa
    12061 taatctactt gtaaattgat tgttgcaaat ggataaggtt tatttaaagt ccaggttgca
    12121 ccacccatca gctcgtctaa cattgttgcc ataacgccac cgtgcaatat tccatttggg
    12181 tttaattgag tttctaaaat tgtaaactca aaaattaatt gtccttcatt tacttcaaca
    12241 atactaccat ttaaaaattt tatgatagga tagtgttctt tccataaatc attaccaatt
    12301 cctcttctaa aaaatgcgac cctttcgttc atatttaatt tattttacta tttcatcttg
    12361 ctccatttct gaagtagtat ggaaatttat ctttgggtaa tcttgttttg tcaacgtata
    12421 tatccattca ctagttgcta aaaacactgg attttcttct ttatcaaacg caacataatt
    12481 gtattttgtt ttgataaatt gattgatttc atttgcatca tcagagctta tccaaaatgc
    12541 tttataatat tgcattggtt taaaagtaca acttgcacca tattcgtgca acaaacgata
    12601 ttgaattacc tcaaattgta attccccaac cgtcccaatt atttttgcat ttgtaagatg
    12661 tgtagtaaac aattgagcta cgccttcttc cattaattga gaaacacctt tttccaattg
    12721 cttggttttc attgggtcag tatttaccaa ttctttaaaa atttcaggcg aaaagcttgg
    12781 aattccttta aatgttagtt tttcgccttc agtcaaagta tcgccaattt taaaattacc
    12841 agtatcgttc aatccaataa catcgccagc ataagcatca tcaacaatgt ttttgtctga
    12901 tgccataaat gtaacaggat tgctaaatct caaattcttg ttgcttcttg tatgataaaa
    12961 gaatttattg cgttcaaatt tcccagaaca aactcttaaa aaagcaattc tacttctgtg
    13021 attggggtcg atattcgcat gaattttaaa aacaaaacct gtaaattttg gttccgttgg
    13081 ataaataatt ctatcgcctg catttcttgg acgtggcact ggagaaattc ttataaatgt
    13141 ttccaataat tcacgtacac caaaattatt gacaccagaa ccaaagaaaa ctggagcaat
    13201 atccccattc aaataatctg ttactttaaa atcatcataa acgccttcaa tcaaatcaat
    13261 atcttctctt aatttgtcag catctctttt gtccaattgt ttgtctaatt ctggactatt
    13321 tatatcttga attgccacaa attcttcttc tactgtggtt ccagatgttc gatataaatt
    13381 caaattttta tcaaaaatat tataaacgcc tttgaaaaaa gttcctttat taattggcca
    13441 agaaagtggt ctgactttga tgtctaattt ttgttctaac tcatctaata aatcaaaagg
    13501 gtctttccca tctctatcca ttttattgac aaaaataata acaggtgttt tacgcattct
    13561 gcaaacttcc atcagttttt cagtttgcat ttctacacct tttacacaat ccaccactaa
    13621 tattacacta tcgacagcag ttaaagttcg ataagtatct tcggcaaaat ctttgtgtcc
    13681 aggtgtgtcc agcaaattca ctaatacatt tttgtattca aaactcataa cagacgtagc
    13741 cacagaaata cctctctgtt tctcaatctc cataaagtct gatgttgccg atttttttat
    13801 tttattagat tttacagcac ctgctgtttg gatggcacca ccatacaata agaatttctc
    13861 ggttaaagta gtttttccag catctgggtg tgcaataatt gcgaaggttc ttcttttttc
    13921 tatttcttta tcaaaagaca taattatgca aagttaattc tattttattt aagtcaagtt
    13981 taaggattta atatgaatga ctatttccat ttttataaaa tagttcttac aatgaattaa
    14041 ttcagatgtt taaagattta tcgtacattt gtaccctaaa tttaaaatta gtgaaattta
    14101 aaatttttgg tgtattcgta ttaataatga tgtgttttgt gtccatcgca caggatgttc
    14161 attttagtca agctccaaat aattatattt acctaaatcc tgcaaatact ggttttggac
    14221 ccaaaaccaa tagagtggtt ggtttgtata gagaccaata ccgaacagcc tttgctactt
    14281 tttctacgac ttttatgtct tatgatagaa gattagtagc ttggggtaat gggtggcaat
    14341 taggtggcgg ggtagatttc ctttacgaca aagtaggtta tggaattata agtactttca
    14401 gtccaaatat ttcattggct atcggtaaat attttaatca aggcagacaa ttattaaata
    14461 tcggaattag ttctggtttt gcttttaaaa aattaaatta ttcaaatctt acttttgata
    14521 atcaatatat accgcttatt ggatttgacc caaatagtgc ttcaggggag caattggata
    14581 acaaacatag aatttatcca aatttgaatt ttggtattaa tttttctacg gcattaggcg
    14641 attattctaa gttagatatt ggagctgcct tagccaattt acatcaacct aatcaaagct
    14701 ttttagccaa ttcagtaagt agattaccag cacgatatac aagttatatt aaagcattta
    14761 taggttttgc taataacgaa aagtggagtt tacaaccagc atttgtatat aattttcaaa
    14821 ataaagcaca aaatatctta gtaatagcaa atgttgaaac aaaatttaag gaaaccaata
    14881 gagggaatta tttaggatta ggttttggtg caggttatcg aattcaagat aatgatgcaa
    14941 tgatagcaaa tatttcagtg ttattaggag atttaagaat tgctacaagt tatgatttta
    15001 atatatctaa gtattaacct gcaaccaaag gttttggtgc atttgagctt tcattaagct
    15061 atgaatgggg cgaacatgaa aatgcacaaa gaagtaagaa ctttaagttt ggttgcccag
    15121 ttgtgctata aagaaaagta cactctttag cgttttcttc aaataattaa cagaaattat
    15181 atttttttac cactatacta aaaggaactt aaaataattg tatatttgtt ttataattac
    15241 gtagttgtat gaattttaga attattatct tctctattat ctgtattttt atctatcagt
    15301 taggcttctc acaagatcca cattttagcc agattcaata taatcctatc tatttgaatc
    15361 ctgcatttgc tggttttgca aagaaagaca atagagtagt tggtgtttat agagaccaat
    15421 ggagaacggc ttttgttcct tactcgacaa cgtttggttc ttatgacaga aagttcaagc
    15481 aatgggaaaa aggttggaga cttggtggcg gaattagttt tttatatgat agagctggtg
    15541 aaggtatttt aagcactttc aatcctaatt taactttagc tgttggaaaa tatttcaaca
    15601 atgaaaaaca attattgaat tttggtatta cttcaggtat aactattaaa tctctaaatt
    15661 attctaaact gacttttgac aatcaatatg ttgctgggag tggatatgta ccaggaagta
    15721 cgccaggaga atctttttca aataataatg tgtcctatcc aaattttaca ataggattga
    15781 attttacaac taaactagga gaaaaatcat ctttagatat tggtggagct gcaagtaatg
    15841 tacatcaacc caaccaaaat ttcttatatt tttctgagtc aaaattacct gctagatata
    15901 ctgcttatgc aaaagcatta gttggtttag gtaagagtga taagtggaat ttacaacctg
    15961 cttttattta taatttccaa aacaaggcaa ataatatttt agtaaatgct attgcagaga
    16021 caaagtttaa agaaaataat aaaggaaaaa ccttaggatt aggatttggt gcaggtttca
    16081 gagtacaaga tgtagatgct gcaattgcct atatttctgt tttatatgat gatttaagaa
    16141 ttggtgcaag ttatgacatt aatttatcaa aatataaagg tgctacaaat acaatgggtg
    16201 cttttgaact tttattaagt tatgaatggg ggaatagtaa gaagaaaaaa tgtacacctg
    16261 atactattaa aattaaagaa tttgaaatcc ttagagatac tgtatatctt actgatacaa
    16321 ttattcaaaa ggatactgtg attcaaataa aagaaactac tgttactcca gaacaaattc
    16381 aaaaatggaa tgaaagcttg agtaagttct taccagcaat agcgtatttt gataatgatt
    16441 atccagatcc aaaatctcaa ttaacaacta cgaatgcaaa ctataaagat ttatatgaaa
    16501 attatatttc aaaaagatct aagtttgtag attatattgg agaagaagaa acaggtaaat
    16561 tatttgatac aatcggaact gaatttaata agttgattca aatttatggt gctttaaaag
    16621 aaattttaga tagaggcaaa gacgtaaaat tacaatttgc aggttatact tcgccattat
    16681 ctacagttgg ttataatcaa aagttatcta gcagaagaat tgaaagtgtg atgaactatt
    16741 tatttagttt agaaaatggt tatttgaaaa aatatttaga caataaacaa ttgacatttg
    16801 aagaagtgcc acttggaaaa agtaaagctc ctgaaagtat cagtgataaa gcagatgatt
    16861 acagaaattc agtttatggc agaaatgcaa gtttgcaaag aaaagtagaa ataaaagcca
    16921 tttatattaa ataaaataga tagagcagat attaaaatag ccaccattcg gtggctattt
    16981 ttattgaaat tgattttttt aagtcgttga atgatgtcaa attcttttaa tcttcttggt
    17041 tacttatttg tttttaattt tattatcaat gtgaactgcg atgtaaaaat aaatgagagt
    17101 attgaaaatt tttgtaattt gacttttgca acagctaaat atataaaaaa atagctgcca
    17161 tatagcagct attttattga taaagattta taggttgcta gaaaatacta aataactttg
    17221 tttgtttctt tgagttatct ttggtatcaa taacttccat aacataattt ccttttggat
    17281 agccttttaa atcaaaatat gcttggtctt gaatgtcttt gtttattaat ttttgtccca
    17341 ataaattgta tatattgaca ttgtaaccac catttttatc cattacaata ctaaattctc
    17401 cttgacttgg atttggaaat aaattaaaat caaaattggg tttcttcata ggattatttc
    17461 taattccaga aattacttta gttgctccat aaattggtaa tataatttgt gaatcataaa
    17521 tatcagatat tgcaacactt tgtacggcaa ttcttggggt atattctaca cctttataga
    17581 tatttgtttt tccgtcattt ggtgctcttc taaagaaagt gccatcttca attggggtat
    17641 tcggatttgc ttgaaatctg ggataattac tactgcaaat tagaactttc attttgtgtc
    17701 cttctgcgaa ggtataggcg attgggtaca tttcaaagaa gaactcatag atttgaccta
    17761 cattaatatt ggtaaatggt gcgttgatgt tttcatttcc ttcagcaata cttttagccc
    17821 attctcgtgc tctcgcattt atgcctcctt ctacgacata aaattcactt ccatcaggat
    17881 aaacgtctaa tattctaaca taaaaatcac aatccgtttc tccattttgg acatttaatg
    17941 gatttgattt tgcatataat tttactttag gaaatccaat aatggataat gaatctgaaa
    18001 gtacatctgt ttcaaattta ataacacctt ctctatccat tgtaaatgaa gaaaaattgg
    18061 ggtctgctaa attcatttgt ccttgactat ctctatctcc ttgtggcgtt tttaccaaca
    18121 tattatttcc accaactgtg atgattgggt tattaggatt gtcagtataa cttactagac
    18181 cttcatcttc tgaaggcatt tcaaaactca atccattatt tctatgcaag tataaacttt
    18241 gccacacaac atcgtcaatt gggaatttgt ctgcacccat ccaataattc cctgcaccca
    18301 agctcatacc ttgattatca gatggcccaa ctacatagaa tctgtaggct ggaatgtctt
    18361 tgtattctag tttttttaag ctctctaatt tttttgaaga agatatgcct ttaacaatag
    18421 catcatctag ttttggaata ttaatattaa tgtttaaagg atttagattg cctaatttta
    18481 tagaaacagg aacttgtttt aatccatcag ttgccaatag gaaattcaat aaatcaataa
    18541 atttcatttt ataatcttta gctggaaatt gtacttttaa atctcctaca acagtttgcc
    18601 aagtattact ttttggaatt aaaatcttag gttcaccaac attattttgt ccaaaataat
    18661 ttaagttata tctaaaccac gtcaataatt cagattctgc aattgaacca acatctaaat
    18721 catctccaaa attagaaata tctacttttg taaaatctgt tacatttact ggatatgttt
    18781 tatctccagt ttttctagaa cctatggttt ggtgagccca tggtccaatt acaatttttt
    18841 gtaaattctt tttatgagta agatattgtc tagcaaaatt attggtttca atttgaccat
    18901 cgacaaataa atcccaccag ccagcaactt ggaaagatgc aacttccata tttgtatatc
    18961 gtgagaattt tccgttttta tccccttcgc cattttcatc aaccatagct acacttccat
    19021 ccatattatc tcgatttata gaattaggaa aataacctgg tgtacttcct gggtacttgt
    19081 aagtagaaaa atgatcaaca gcagtttctg ccgctacaaa cttattaggt aaattataat
    19141 cttttgccgt atgaatcgaa tcattaatac cttggtctat ggacatttta tcgtcttcta
    19201 aatcataaat ctgtcctttt aaccaacctt taaccaacat atttctaaag caaccattat
    19261 gataaccagt acttttataa aattctagag gtccaacaat cggaaataaa ctttttaaac
    19321 ctggttgagt ggggtcaatt ttatgtgctg ccgcagcttg gtgttgatta taacctaagg
    19381 ctgatgcccc aaacattcca atagaaccat tatagactaa atcagttgtt tctagtgtac
    19441 catttttatc taaatcaaat tttctaacta aattattttt aataaactca atggtattgt
    19501 agccatcttc gtgattattg ccatttcttg ggtcgtcagg tgaagttata tctaataagt
    19561 gattgttatt atgataaggt gttttcttcc aactatcaga gtacaaaggc aagtaagcac
    19621 cttgtgaagt atagcgtcca cgcatatctt gaactgctgc tgagtaacct aataaagcca
    19681 ttgcagaacc aatcatcaca tcacctccat ttttattata aggcgttctt tccaaaatca
    19741 tgggcaattg ataaggattt ggatttagtt gtccattgat tgaatcatag ataatatatt
    19801 gaaaaccttt tcctaaaaca gttagtttta tatcctgatt gataatagga attgtaacat
    19861 caaaagttaa actatcttgt agaataggca aatagatatc ggtatataaa attgtactat
    19921 caggcatcat aaaaggaact tgcactcttt gtgcaaaatc tgttaaatca tctagcgttc
    19981 catttacttt tggagctgga attaatttgc cgtttggaat attaagttgc tgtgccttta
    20041 aattgataaa taataagcaa acccaaacga aagacacgat gatttttctc ataactttaa
    20101 gattatttaa ctaaagtaat accatttaat aaattaacaa aattttttgt taaaaattaa
    20161 tattggagta tttggataat agctatttag tcttttaaat tataaggata ttaaaaagat
    20221 aattttttca attttgtata aaaaaagtta gtctgtaatg tgaatggaaa atatattatt
    20281 tcaaatattt atctattagt tcattccatt tcagcttatg aattttcttt atttcgttgt
    20341 cgttttgaac taattcatta taatttttta caaactcatc taatgaatca atcaaacgta
    20401 aataatactt aatagttggt aaaagtccat tgataatgat ttgacaacca tgttcaaatt
    20461 ttaattggtt tattaaatcg tctatttcta atttatcagt ttctttaatt cccaaataag
    20521 acaaaatgta atatctgttg ggattgtatc ttgcaatttt ttcaatagca attcttacca
    20581 ttgtaatatc aattgcttta tctaatttta tttctattgc ttcaaatagc tctttctttt
    20641 tgaaaatttc aatatctcca gctgattttg atgttctgtc agatgcagta tgacttccca
    20701 tttcagctaa agtacaagat ttatatcgct ctatttcctt gattaaactt ttgtagatag
    20761 cgaaaaatgc taaaactgga agtttagaac ctccacttgt tgcatatttt gttctaaaat
    20821 gttcgtccaa tgctgaaata atcttttcga ttgttagcgt ttcggggttt tctaacggaa
    20881 taatatcaac aagtgcatca ttttttgaat ttatagcttc attcaataat aaacgtagaa
    20941 cgtttttagc tgactttggg tttctttcaa caaagtcaag aacatttaaa aatgcgtctt
    21001 tcactatttt gttactaatc tttccattgt aatctaaatt atatggataa ggttgttcta
    21061 aagaacgtgt tagccaacca ctttctgcca ttgatggtaa cttaagttgc ttcaaagttg
    21121 gtgtaataaa aacggtatct attgaacgac cagaaaaacc accgtccata cttgtttgat
    21181 gttttctaat atcttgtttt ggattgatga ttttgtgtgt aatcaaagta actaaaacag
    21241 taaaaacacc tttttgagtg tttatctttg ttccgataga tttaataaaa tctttggtct
    21301 ttttatttac atctttaata tccgtaacta tggaagagcg attgtaaatc tccaacaact
    21361 tgtctttgtg gttcatctat caataagttt tgttctttta tactttcggc tattgctctt
    21421 attacaggaa ttgctaccga atttccaatc tgtttgtatt gttctccaat gtttgagtgt
    21481 tttataaagt cggacttaaa acccattatt ttataacatt cgttaatcgt caattttcta
    21541 accgcatcta attctggaat ataaataaaa aatctccctg ctgtttcttg ggacggaata
    21601 gtaggatgtg ttccgtcaat cgaataaatt ctattaggtt gtctgtgaac tcttgaaaga
    21661 tgttctgtat tttctctaat cccgtttttc caagtatttt tatttctata accaacaaag
    21721 agtaatccac tttcttgttt tttaggtgtt tcaattaaag tgtattctga tttttctaaa
    21781 aactcaaatt ccccttctgt atctaaaaaa tctctcaaaa taactttatc tttcttttct
    21841 aattttgaaa aattaaaata atattttttt gtggctatga tgaaaattct ttctctgttt
    21901 tgagggagtc caaaatcttt agcgtttaaa atttgatgag ttacattata tcctaaatcc
    21961 attaaggatt ttaagattac agtaaatgtt ctacccttat cgtgatgcac taaatgtttt
    22021 acgttttcta acacaacaac atttggttgt tttttgtcaa taatttggca aatatcaaaa
    22081 aataatgttc ctctagtgtc ttcaaagccc atttttttgc ctgaaatact gaaaggttgg
    22141 caaggaaatc cacctaacaa aacatcgaaa ttaggaatgt ctgtagggtt aattttagtt
    22201 atatctccaa aaggtttttc tccaaagtta tttttataaa cttcttgaca aggcttattg
    22261 atttcagatg tgaaaacaca tttaaaacca gcatcttcaa agccttttct aaagcctcca
    22321 attcctgcaa ataagtctat aaatttatat ttcatcttga tttacgttcg tctttttcaa
    22381 atttacagat taaagattgg tcaatatcaa gttcagaagc aatttttcgc aagatgatat
    22441 tcttttcctc tcgtgtagat tttaatattt tgcataatac gcttattttg atatttcttt
    22501 ttttgactaa tataatcaaa aatacaattt ttaaattaat taaaatgcca attaatatct
    22561 aataattcta tatgtggtaa atacgagtat gaaattttag ataattcatt aataagttaa
    22621 cttatctctt ttaaagtagt ttattttcaa agttatttct ttttttatgt aagaagatgt
    22681 atatttatta ttatcttaca atatgtattg gaaactaaaa tatttattga cattcctaaa
    22741 ttttatacaa cctacaagaa aagtagaatt atcaaaaata aatgatgaaa gaagaaagaa
    22801 tatcaaagtt tcccaattag gaaaagtttt gtttgataaa aaagaggcag ttttatctgt
    22861 tgttgatagt aaaatagagc atcttcctat tagaatatat aaaaacagta aacaagaaca
    22921 tcaaaaagtt atagtttatt ttcatggtgg tggtttttgc ttttataata tagaatctca
    22981 cgataatata tgtagaagat tatgcaaaat gaatgattgt acagttattt ctgttgatta
    23041 tagattagca cctgaacatg tttttccatc agctcaagaa gatgcatttt tagtgattga
    23101 atatattcat cagcatgcta gaggatttgg aattaattca aataaaatta ttgtttgtgg
    23161 cgacagtgct ggtggaactt tgtcagcatg tgcaacgcat cactttaaaa atcacccaga
    23221 aataaaaatt gctggacaga ttcttattta tccttgggtt gatggtcgta taaattcagc
    23281 atcgattgag aaatttagca ctggttattt gcttaccaaa gaagctattt tgtggtttca
    23341 aagtgtatat acaccaaatg tagaagatag acttaatcca ttatccgcac caacacatca
    23401 taccgatttt tctaacttag cacctgcatt tattgccact gccgaatttg accctttaaa
    23461 agatgaaggt ttagcgtatg cagagaaatt aaaagttgct ggaaatactg ttttttataa
    23521 agattatagc ggattgattc atggttttgc taatattcca aatattgcac ctaatggaat
    23581 gaacctattt gatgatatta aacaatttgt cgaagagctt taaagttaaa tccaatacat
    23641 tattctgaaa ataatttaac tttttcctaa aaatagactt agtaacttta tcctatgttt
    23701 ttaaatttat tcagggcaaa attacacaga gttactgtaa cacaagcaga ccttaattat
    23761 agaggtagta ttacaataga cgaagcactt ttagatgctt caggtattgt agaaaacgaa
    23821 cgtgttcaaa tagtaaatgt caataatggt gagcgttttg agacttatgt tattaaagga
    23881 gagagaggct caggagttat ctgcctaaat ggaccagcag ctcgaaaagt acaagtaagt
    23941 gatattctta tagtcattgc ttatggattt tattcggctg aagaagcaaa agtacatgaa
    24001 cccaaaactg ttcatgtaga tggaaataat caaattattg cctaaaatca ttttataatt
    24061 tgaaaaattt gagctctatc ttaaaagttt tgctttctct tgctttggga attggagcaa
    24121 tgatttggtt ctggaattct atgtcagttg aagaccaaaa tcaaacaatt tttgcattta
    24181 aacgggcaaa ttatttttgg gtaatattgg caccaattat tgggttttta gccaattttg
    24241 tacgaactca aagatggcga ttgctattgc gtccagtagg atataatcca caatatacca
    24301 acacttttta ttcggtaatg accatgtatt ttttcaatct atttgtacct agattgggcg
    24361 aagtaatgcg ttgttccatt ttagcaaaat atgaaaatgt tcctgttgaa aagtctattg
    24421 ggactatggt aacggaaaga gttttagata tattatgttt aggtatagta tttgttattg
    24481 tatttttatt cttaggacaa gaaaattatg atatactaaa agccaatttt aatgaacgta
    24541 cttctggatt tgggaatggt gcaatttttc aaattttaaa atttagtatt ccagctttaa
    24601 ttattatagg tggaggtatt ttttctatta tctatattaa gaaaaatgga ttaaataaat
    24661 tattagattt attcaaagac aaaattggta gtctttttaa atcaatattc tccgtaaaag
    24721 atgttgctga aaaaccacaa tttatcatcc ttacaattgg catgtggctg ttatatttgg
    24781 ttatgtttta tatcaactat ttagccttac cagaaacatc agatttgcca cttttctctg
    24841 cattagtttg tttattattt ggttcatttg cagtgattat tacacctggt ggcattggtg
    24901 tttatccgat tgttattcaa atggttttgg ctactttcaa agttgaacca agtattggtt
    24961 tggcaattgg tatgattgca tggtgcgttc aaactgttgg tgtgctttta ggtggaataa
    25021 tttcattggt attgcttaat ttaaccaaca gaaatatttc agattctaat gtcagtactc
    25081 attaattctt tagaagaact tactataata gttaaacatc atagagctgt taaacaaact
    25141 attgttttta caaatggttg ttttgactta ttacatgagg gacatttcca tttattaaat
    25201 gaatctaaaa aattaggaga gatattaata ctaggaatta atacagatag ttctatcaaa
    25261 aaattaaaag gagatacaag acctattcaa aaattagcag agagaattga tgctattaaa
    25321 aagaagtcaa tagttgatta tataataaca tttgatgatg acacacctat aaaattaatt
    25381 gaaattattg agccagattt tttagtaaaa ggtggagatt atcaaattga aaatattgtt
    25441 ggaaatgagc tagccaaaaa aacaattatt attccattgt tagaaggttt ctccacgaca
    25501 caacaaatca ataaattaaa gcggtaatta ttttttatct tttttgtgtt ccaattttaa
    25561 aggtggagca ataacatctt caggcaactc gacatctttt ttaaacaaat cagctcgagt
    25621 aaaatattca gtttcatcaa ctttgacttt aattggaata aaacccatcg ccacaatttt
    25681 gatatatcct ttgtaagaaa ctttaacagc ttttcctata gccaaacgag ctgattcaga
    25741 aacaaatttg gaaagcgttg caccagtatt tacttcgatt cgaagtggta aatcaaaagt
    25801 atccattttg ggaactttca cataatctgg atgttcaaaa atagtaccta attttttgtc
    25861 ttccacaaaa acatcgacaa caacttcttc aatttttgcc tttactttat atggactata
    25921 aatacaagca gtaattccta aactaatttg ttttatacca aaatctaaaa ctttgaaatc
    25981 tttagtgcga actaaataag gttctttttt ttcttcagca tcaagtttga aagaaaatcc
    26041 aatgaagata attaataata atagtaggcg ccgttgcatt tagcttttag attttaattt
    26101 ttctttctct gctttttgaa gagctttttc tgcttttctt tgttctcttt tttcttttcg
    26161 ctcttctttt ttagttttgt aagtatagct tgatttgtgt ttcaaaggta cttcaaattc
    26221 tttgccagct ttatctctaa gtgttaaaac accttccatt tcaattaaat acacattttc
    26281 aggatcttca ttgttgccaa ttactggttc agttttatat tcatatttta ctggaattga
    26341 aaactcagaa tgtcctctta cattttttgc ttctttgtta aaaatagttc caatatcttt
    26401 tccgtctatt tttaaatgta cttttaaatt tctaacatca aactcttctt cagccaaatt
    26461 ttcaaataca aaaacagcca aaagatttgc tataggtttt tttaaattac ccatgtcaat
    26521 atcagcaact tttttaaatt ctacttttgt ttcctgctta caacttgtaa ataaaaggat
    26581 gctgaataga gcaataattg taattttttt catgctatta aaattcgatt taattttcta
    26641 acaataatag tgataataaa ataaatatca ataatgtggc ttattttatt agtatttatt
    26701 aacgactata ttttatgtta tctctatttt ttaaaaagca attttaaaaa atgtttatct
    26761 aataaaataa tgctttgtgg taacaaaaaa tatttctatc tttgcagtcc gttataggct
    26821 gatggtgtaa cggtagcaca gaggtttttg gtgcctttag ttaaggttcg aatccttatc
    26881 ggcctacaca aaaaagagtt tcagaaatga aactcttttt ttgttttatt aagtttattg
    26941 actacttctt tttatagata ttcaattttt ttggatttta cttagcaatc taaagattgt
    27001 taattaatat acgtttgcaa aaaaaaatgt taattttgaa agttgtatgt ttgatagctt
    27061 atttaataaa ttatttttat ttcacacttt tttcttttgg gtattaaata gttatgctgt
    27121 ttgtgatttt caggtagata aaacaagtgt ctgtgcagat gaagatgtta ccataatttt
    27181 agataaacct tatagtgcct atcataaagt aattgtatca aaaggaacct ttccactttt
    27241 gtcagatgca acacccaatg acttctatat agttccacct tattcggcca atgataatat
    27301 tgtaaaaatt agatttcgtg gaaagaataa tatacaagac tatactattc aattagcaga
    27361 aagtgctttt cctaatccag gaattccttg tgggaaaaga gttgttgtca gagttaatcc
    27421 aacaccagat ccacaaataa cagaagaagg gaatttttct tattgcaatg aaagaaatcc
    27481 taaagaaata aagattctaa acaaatctca aactaaaaat accaatactc aatatgaaat
    27541 tgattgggga gatggaagtc ctgtttttgt agcaactcaa tttgatgaag tcttgcatac
    27601 ttatgctgtt ggtgattatg ttattaaata taaagttgtt ggcaataagc caaatacaac
    27661 ttgtaatatt tctgaaaaag aatactatgt aaaaataggc gggatatcac ctagaatttc
    27721 tttcttacct tctacacaaa cttcattttg tgcacctggt tactatgaaa tatatcctga
    27781 cactagcgta atgaatagaa atacgcctaa tacaaattat aaattttatg tcaatgatga
    27841 attaatccaa acctataatc aaaacaattt accaactatc ttaaattatt attttgaaga
    27901 aggttcttgt ggcaaatctt ctcctcaatg tgggcaaagt tccgttagtt tttatttaga
    27961 agcctataat ggaagttgca cacctgcaat tctcaataca tgtgttttta ttacagactc
    28021 tttaaaacct tatattacag gaaaagatgt agtatgtcag actatgactg aatcttttac
    28081 caatggaaat gcaaaaaaag ataaatatat tgcaggaaca caatgtaaag atcctattta
    28141 taaatggagt attctagaat ctaatggata taccttaaaa aatggtacta gcaatataaa
    28201 ttttgatgtt caatttgata gtattggcac atatacaatt cttttgtcat tagatgcatc
    28261 atcaggaatt tgtcaagata aagcggatac tacatttcat gtaagcgtag tagaaaaatt
    28321 agttttagat tttaatttgc catctattat ttgtatgcct aatatcgaag gtagttttgt
    28381 agatgtgcct attaatttag aaataaaaga agggaatcct tcagggataa aaaagataac
    28441 ttggacaata gtccctgata atactgctcc gataaatgtt cctaattatc aaattatttc
    28501 aggaaataca actgctcaaa atatgcttat ccgatttgat gcttggagaa gttacgatat
    28561 aattttaaac tatgaaacta tttgtgaaac tggtactatt gtccgaaaag ttaagatagc
    28621 atctaaaggt gcatttgata cacttcctat cccgagtatt tgtgcctatc cagcaacaat
    28681 aaatccttta gaatatttta tttgggaacc caaacgagta aatggtggcg ataaacaagc
    28741 acaatttaat tggactttta atggtggaac tcctagcact cagcaaacca agagcccaaa
    28801 agtcgtatct tataatgcaa gtggcactta tccaatgact ttaacaataa caaatgtatg
    28861 tggcgatact tcactctctc caaatgttat attatttgat aatccacagc ctgatgcagg
    28921 aaaagataca actttatgtg gtatttcaaa tcctattaca ttaataggaa cacctagtgg
    28981 aggtatttgg cgaggaatag gtattattga tagtgtatta ggtatctttg atccaaaaat
    29041 tgtgtcagcg ggtagttata caatttttta tacaacccat attaattcag aatgtcctcc
    29101 aacagattca ttaatagtta cagttttgcc aacgggaatt tccgcaggtc ccgatcaatc
    29161 aatatgtaga atcaatggca atcaacaggc agttttacaa ttattgagta atcctacatt
    29221 tccaggaggt tcttgggctg gtgctggtgt tattaattct gctagtggtt tatttagtcc
    29281 tacgaattta acacccggaa cttatgctgt tcaatataca tatacagatt ctgtaagagg
    29341 gtgtaaagat gtttctagta aaaatatagt tgtccaccca gctgtgagta taaatatgca
    29401 acttcctaat ttatgcactc aacaaacttt tgttttagtt ttgtaggtgg taatttaata
    29461 gatgcagatt gggattttgg tgatggtggg aatgccaata ttccatatcc aaattatatc
    29521 tatactaatg caggagatta tacaattcga ttaaaagctg aaaattcaaa ccattgttca
    29581 gatactttta atattccaat aaaagtttta ggagaacaaa gtcatgaatt tcttatcaat
    29641 aatgctacct catgcaatgg cagtgatttt gtaatctctt ttcctccaaa tcatgatgta
    29701 gcacaaacct acaaatggta ttatgataag gattcactca taagtaatca gaaagttata
    29761 ccaacactga attttactaa gcctttagta aacgatacag tttatacagt aaaattaatt
    29821 acaaaatatc aatgttcaga atattcacaa actttagatt tgaaagttaa agctaatcca
    29881 aatgcaaata tctttctatt atcggataaa ggctgtgctc cattttcaac tcaagtactc
    29941 aataattcaa caggtcaaat tgatagtgta agatggtttt ttggtaatgg acaaacgagt
    30001 acaattgttc aacctcaaca aactccaaaa tattataacc ctactagagc agatacaagt
    30061 tatacaatta cattactaac ctacaatatt tgtgcatccg atacagcaaa aaaagaaata
    30121 gtcgtaaatg ctaatgaagt ttatacagca tttactatgt cgaaagatgc aggttgcact
    30181 cctttaactg ttgattttac atctacagtt gtcggtgcaa atagttttat ttggaatttt
    30241 aatgatacag atagtagtat aagttttgat aaaaacccaa cattcacatt tgataaatca
    30301 ggaatatatc cagtaatgtt gattgcaaat gggagttgcg gaagcgatac cattaaaaga
    30361 aatgttacag tttttggtat tcctgatgct aactttgaaa ttataaatgc ttgtgttaat
    30421 caaaatgctt tatttcaaaa taaaagtact gatgcaaata gttttacttg gaattttgga
    30481 attcccaatg cgacttcttc aaattctaat cctactttta aatatacaca ggatggtgct
    30541 tatcaagtac aacttattgc aagaaatact ttctgtgcag atacgatagt tcaagatatt
    30601 cttgttcaat ctacaccgaa agcaaagttt tctatattta atccagaagt ttgtttttct
    30661 gaattagttc aattcaataa tgaaagtacc aatgcattta attatacttg gcattttggc
    30721 gataatgaaa tttcagatgc tactaatcct cagcacatat ttccaaatcc aggaattttt
    30781 aatgtttatt tagttgctaa aaatactttt tgtgcagata gtgttcgttt aaataatgca
    30841 gtacgtgtaa atcctaatcc tactgctgat tttaatgtaa tgattataga taatgattta
    30901 agaaaacctg ttcaatttac gaatatttct actaatgcag ctcaatatgt ctggcagttt
    30961 ggagatggaa attcttcaac tgaaaagaat ccaaaccata tatttcaata caataaaggc
    31021 tataaaatcg ttttagaagc cgtaagtgat aaaaattgta gagatactat tgagaagagt
    31081 atcaacatta atccaagtgg aacactttat atgcctaatg tttttacacc aaatgggaca
    31141 ggcgaaaatg ctttttttaa acctaaaggc aataaattga aagaatacca gattcagatt
    31201 ttttcaactt atggtcaatt ggtttgggag tcaactaaat tggctgatgg tagtccagtt
    31261 gaagcttggg acggtacatt caaaggagta ccaatgccac aagatgttta tgtttggaaa
    31321 attagagcaa tttttgatga cggtactttc tgggatggga tgttagatga aagtagcggt
    31381 aaaaaatcgg taataggttc tttaatttta atcagataaa ttatagcatt attttttcag
    31441 cctcagctaa tttttcctga ctgccaatat ctatccaaat atcatcgtta tgaatataac
    31501 tttttattaa atggttagga cataaatcta aataccaatc tgtcattgaa aaagccccat
    31561 ttctgttgca atgtttaaaa atttctggtt caataatatg gtacccactg aaagcaaatt
    31621 catgtaatgg atgctctatc agtttttcaa ttccatcatt atctataaaa tattgtctcg
    31681 gagattttcg tggaattttg aaagtatgtg tttttgcatt ttcccaacca aataaaactt
    31741 gctcatcgtc aaataataaa taccttgatg aatttctatt gcgaacagcc aaagttgcca
    31801 agctcttatt ttcttggtgt tttgctaaaa aatgttgtag gttgatgttt gtataaatat
    31861 cagtattgca aactaaaaat ggttcattac tttctgtaaa aaaggattta gcttttaata
    31921 aaccgccgcc tgtttctaaa attaaatctc gttcatcaga aatctcaatt ttaattccaa
    31981 actgattgtt ttgctgaata aaatttataa tctgctcacc taaataatgt acattaataa
    32041 taatttcttg aataccttgt tgttttagct gctcgataac aatttgcaac ataggtttcc
    32101 catttatcgg caccaaagct ttaggaattc tatccgttaa tggacgcaag cgagtaccta
    32161 aacccgctgc aaaaatcatt gctttcttca taaaatagta attaggaaaa agacattagt
    32221 tcaaagtatc tatataataa ttcaataatt ttatctttca tttgttgtct attgtatcat
    32281 ttattatctt ttaactcatt attgcataga ctttttagta ttgcaatttc atcatctttt
    32341 gattgatgca aaccgctcat aaagttcaac agttttttcc gaaatttgtt tatttataga
    32401 gataccttca ccattatgtg tgtgataatt attgtaaata ttgaaagaat ctgtatttag
    32461 taattcatat acggttgttt ccaatgccga agctatccta aataaattat ctacagttag
    32521 ctttgtttct cctgtttcaa ttttgctgta tgctttctgc gaaatgccaa gttcttgtgc
    32581 aagataagtc tgagaataat ttttctgctc tcttattgcc tttatccttt ctgctatatt
    32641 gggtactgtc attaggtact aacttagaac tttaaatgta ctaattaagt atcaatccta
    32701 caatagtttt cttaaattat tttataaact tgaaaagcta aactatctaa aatcaaaaat
    32761 ttatgacaaa aattaagatt acagtaccca aggtacattg cgttaaaaga aacgatttat
    32821 tcggcaaaga tgaaatcttt atggccattt tagtaagcac agcaaagcca acagacgaag
    32881 cacttgaacc ccaaattctt ttttgggccg ttagtaatac tctgaaagtt gcttaaggta
    32941 aaactaaacc tttagcagga gatagctgga tagtagatat tgacgaacat caatctattg
    33001 atatttcttt tggttaatac gataaagata aaggcgattt gtataaaact tatcaaactg
    33061 attttagcga gattataaaa caggaaaaat tgggagtaga attaaaaaaa atattgaaag
    33121 atgttgtgaa agacgtagat ggatttagta aagaaaatat acttgatatt ttagccaagc
    33181 tcgtaaagca aatttttcag caacttcgta aagatgatta tttaggaagt aaatctgaaa
    33241 gttaagaggg agaagataag acttcggaat ttgatataaa accggacaga aaaacacatt
    33301 ataaagttgc tttaaatgtg gaaaatatca atgaataaaa aaacaaaaat ggcataaact
    33361 tattaaaaaa caaatttaaa catataaaat tatgaaaaaa ctaaaaatac tttcaattct
    33421 gctgtttggc attcttgctt ttgcttgtaa aaaagacaat accgacaatc caccaaatac
    33481 agacacaaat cttacccgct tttatagtgt ggaggaggct gccgaagatg ctttgatgct
    33541 tacaagttta gactcctcaa gaagcattat gctgattgct tatgccgctt ttgatgctga
    33601 cggaattcct gcccgcatta atagtttagt gttgggcaag gtggctgaaa attatcaggg
    33661 caatcttata tttgatgagc aaaaacgtcc tcaattattt tatgttactg ccaaaaacgg
    33721 aacaaaagac aatgtactgc tgtctttttc ctatcctgca aacgataccg tagtgtatag
    33781 tttatattat tataattgga gtacacaaga agattcctta ttgcaacagc aaaaaattaa
    33841 tatagagaac tttaacagca cgtttactta tggcaacaga ttagctgagg tggcatattg
    33901 gaacaaagta gccgatgaat tggaaacagt acacaacaat atggaaaaag tttttaatac
    33961 cgaaatagtg ataaaagctg tttcagacac acgatttaat acgcaaaacc ttgccaattt
    34021 tttagaaaca acagcagatg caaacaggag taatgccaga attgcatttt cagctattct
    34081 ttataaatat ttattaaagt tagagcaaga tagagtccat gctcaacttg cagcattaag
    34141 tccggaagat tttaaaaata tgaaagttgt agttcacccg aaagggttaa ttcctaaagc
    34201 agaatatatt tatcccaaag aatctaaatc gcttataaat tatatatatg cactgtttgg
    34261 tattggcagc cccactgcgg aagcagccac catacccaaa ccaattggca caccagaaaa
    34321 tccgaatggt ttgccaagta ctggaactgt tacggatata gatggtaatg cttataaaac
    34381 cataaagata ggcgaccaaa tatggatggc agaaaactta aaaacatcga agtacaatga
    34441 cggtacagaa ataccaaatg taatagatga cgaagaatgg gaaactctga cggaaggtgc
    34501 ttatacttat tataacaagg atgccaatca taatgccact tacggcaaac tatacaattg
    34561 gcatgcagtg aacactggca aactgtgtcc acaaggttgg catatcccta cagatgcaga
    34621 atggacacag ttaataaatt atttgggtga agatgaagca ggacaaaaaa tgaaatctac
    34681 tggaaatagc acagatggca caggattatg ggataaggat ttaggctatg aaggcaccaa
    34741 cgaaagtggc ttttcaggtc ttccaggcgg ccaccgatat ggcgatggct cgtacggtat
    34801 gggcgcctac ggtggctggt ggagttctac ggagggcggt acagaccttg cttggggtat
    34861 gttattgttt tacgattaca atgaagtgga cagtgaggaa tacaataaga attatggttt
    34921 cagttgtcgt tgtgccaggg attagactct ttgttccttt gacactttga caaaaggaat
    34981 aatagggcga tggccactaa tttttcctca acccgtttgg gtaatgtact gaaaaatggt
    35041 tggtagcttt taagaaaaaa agtgttatac ttcggctgtc aatcagaaga ggaagttggc
    35101 tctgctgagg agcaaccaag cagcaataag attgatgctt atacgaagaa tgaggacagc
    35161 ctacaagctg tcctttttta cattctttgg taagcaaaat ggctaaaaaa aaacctattt
    35221 tttgtttgaa aaataaacat accatttgat aaagtttatc ctatttttaa aggttaaccc
    35281 tctgtgcaaa tccaaatgat ttttcaagtg actaaagtat ccttctatac catttgttgt
    35341 attaggaatc tttggattct gcaagtaatg gaacatatta ggtaaggctc tttttaataa
    35401 aaaataggaa cgtctgacca atttatgagt gtaccagtat cttcctgttt tctcattata
    35461 ggttctttcg gaaagataat cctgatgtct tttataccaa agattaagtt catttatcca
    35521 aaataatcta tcgttttccg ttttaatttt aagtaatacc aaatataaat attaaacaga
    35581 ccaaaatgta tttgaaaaaa tgtatttgaa actgcaaatg cttttgactt ggtctcaacc
    35641 cttgttggtt tgtaaccaac atttcaagaa aaacactatt ggttgcaaac cataaatgat
    35701 tataaaaaat ttagaaataa aaatcttgat tctaaaagtc taaaatctat aacaatgccg
    35761 caactcctgg taatgttttc ccttcaaaat attcaagcat tgcaccacct cctgtactta
    35821 caaaacttac atcgttcgcc aaattaaatt tatttacagc agctacgcta tctccaccac
    35881 caactaagct gaatgctcca ttttttgttg cttcaactat tgctaatgca attgctttag
    35941 tgccgttttg gaaacgctcc atttcaaaaa cacccatagg tccattccat aaaatagttt
    36001 ttgatttttg gatgacttca gaaaatgtag ctatggcatc tgctccaata tctaaaccca
    36061 tccattcttc aggaatattg aaattatttg caatttgtgt ttgtgcatca ttgtcaaatt
    36121 tgtcagcaat tatactgtct tctggtagta taatttgaac tcccttttgt tttgcctttt
    36181 ctaaaattcc taatgctagt tctaatttat cttcctctac taaagaactt ccaatctgtc
    36241 cgccttgtgc tttaaaaaaa gtataagcca taccacctcc aataataata ttattggcca
    36301 tatttaataa attttcaatg attaatattt tatcagaaac ttttgcacct cctaaaattg
    36361 cagtcaatgg tttttcagca tttttcaata ctttatcagc attttctaat tcagcggcca
    36421 ttaacaaacc aaaacattta tgctctctat cgaaataatt tgcgacaata gttgtacttg
    36481 cgtgagctcg gtgagcagtt ccgaaagcat cattgacaaa aattgttccc aactttgata
    36541 attgttcagc aaattcagat tcaccttttt cttctgcttt atgaaatcta gtattttcta
    36601 gtaataaaac ttcgccagct tgaagtgatt gtgcggtttg aagtgtttgt tctccaatgc
    36661 aatcagttgc aaattgaaca ggtgtattta ataaattaga tagatgattg acaatatgct
    36721 ttaaactaaa tttttcgtaa tctattgaac catcttcttt taattttttt aatggtctgc
    36781 ctaaatgcga cattaaaacg acacttccgc catcatttaa tattttttgt attgttggta
    36841 tagccgcacg aattctcgta tcatcagtaa tattaaattg tttatctaaa ggcacattaa
    36901 aatctactcg aatcaaagct ctttcttgat taaaattgat attgtttata gtttgcattt
    36961 caaaaatatt gtgcctaaaa ataagcaaat ttgacaatat taaaaacaat gtttttttag
    37021 cagaatacta actgctacta caatttatag cgattaattt attcgcctta atctttagag
    37081 aatattgact atcgaaaaaa tcaagtaaca agttctgttt ttgaacaaaa aaatgaaaga
    37141 actaggtttt taatttactt ttttggataa aatatgaata aaatacagat aatattatat
    37201 ctttattcat tacttttaga taaaatatga ataagatatg gataaattca ttcagaaact
    37261 taattttgac tttgcaacaa cccaaagaat agtacagcta attgcatata ttgatggttt
    37321 taaaggaaaa tggaatattg ctgaaaagca aaaaaaccgt tatctgaaag agttgcgaaa
    37381 aattgcaact attgaaagta tcggttcctc tacacgaatt gagggagcaa cacttaccaa
    37441 taaggaagtt caagaacttt taaatgatat taaaataact aaactaaaaa ctcgtgatga
    37501 acaggaagta atcggttatt atgaagtttt agaattgatt tatgataatt attccaacat
    37561 cagactttca gaaagctata tcaaacaact acatcaaatg ttgcttaaat acagtaagaa
    37621 agacgaacga catagaggta tatacaaatc tctttctaac aaagtagttg ccaattatcc
    37681 tacaggagag cagagaacca tatttgcaac tacagagcct gctttggtag taactgaaat
    37741 gcaggaatta gtagagtgga caaatgaaca attggaacaa aatgacattc atcagttaat
    37801 tgtaatcggc agttttattt atgatttttt gagcatacac ccatttcaag atggaaatgg
    37861 cagactttca cgattactta ctacactttg tctattgcaa aatgattata ctttcattca
    37921 atatatctcg tttgaaaacc atatagaaca acacaaaaaa gaatattacg aagtcttgat
    37981 gagtggacaa aaaaatcgga gaacaaatga agaaagaata aatcattggc tgattttctt
    38041 tttggaaagt atgaagacac ttattgaaaa attagaacaa aaatatgatg tattcaaatc
    38101 caaaggcggt tatctgaata acagacaaaa gttgctaaaa gattttatct cgaaaagtga
    38161 acctataaaa gtaagcgatt tagcaaagca gtttcctgaa gtcggattaa gtacattgaa
    38221 aaaagattta caatacctac gggacgagca agttattaca atgattggaa aaggcaaagg
    38281 agcgatttat attttgagcg aagaaacata atccaaatac tgccaaacta aactctctag
    38341 gatattacaa aaaacaaaaa gaagatttag atacttttca aaaactaggg tttaaaattt
    38401 gctaattcta aaatcttctg actgtctttg ttgacaatga gttgttttaa agtggtatta
    38461 gaattatttt tcatataact tctaatgatt tgccaaccca accaagcacc aacccttcct
    38521 ggagaagaat caggcattcc ataagtacta ggagcttctt ctacatattt aatgtattct
    38581 ggttttgtat cgtataagat ttttttctct aagaaatgtc cccaaatttg tttgatatta
    38641 tcctcgcacc atttatactg ttgtggcgta taacgcataa tattagcttc tttttctttt
    38701 ggtaacattg tctcgacaaa gtataatatt tttccataaa ccaacatttt atctaacaaa
    38761 gtagattgag ctgcaggagc atctatcgta ttcgtagcta aagtatgcat tgcttgaatc
    38821 gtaataaacg gcttatctaa actgtacaat tggtaatcgt agaaaaagtt gggataataa
    38881 atatacttag gacctagata atcatcaata catattccta acaaactatc accataggta
    38941 aatgcaggaa acccagcaaa ttctgtaatg caagtatata cttttggaat aggtttttct
    39001 ggaaaataac ttttgaaatt tacaaatgca tcagtcagtt ctttttctaa gaaatctaaa
    39061 tttggatatt tcttcattac tgaatcatat aaaccacgca tttgaggttt ggttataaaa
    39121 tcagtgatga tttgttgttg ttctgcctca cttcccatac gtggaaaatc cattacataa
    39181 ttataataag cattaaatat taacgggtct tttgctttta attgatgcaa ttcattttca
    39241 atattatttg gattcatagc ataccaatct tcctcaaaac gatgtatttt tacttttact
    39301 tccgattgct ttacctcgat ttctttctta ggttgatttt tatttctatt gcaggtacaa
    39361 cttgaaagca acaaaacgaa aagggacaaa taaataactc tttgcatact tggaacaaga
    39421 tacgaacaag aaataaatat tgaaagtaat attttcttaa atgatattaa tgaacgtatt
    39481 tatattcgag ccaaccatat tgaagataag tcagagtatc ttccgtaagt ttacttatat
    39541 aataactagt tactcctatc ttcaatgagt cttctgctga aaacgaccaa ttgtctgtat
    39601 gtgcagcttc taaaccacat tttactgtac ctggatgata ataaaatttt ccatttgtat
    39661 tgaaagtgta catatcatca gtagcacaag cttcaaacag agattgtcca tcattcaaat
    39721 caataaggct tattcttctc cagttctttt gcgtaagtaa ttcagtttta gttttttctt
    39781 ttgtgggttc ctttttacat gcaaaaataa gaactacaat taacagaagc cataagtttt
    39841 tcataattat attgatttta tttgtaatat taaatataat cttgaaaaaa gtcaagcgta
    39901 gaatatatat ttttttactt ttacaatatg aaaattgcat tagcacaaca gaattatatt
    39961 attggagatt ttgaaggaaa ttttgaaaaa ataagtgatg cgatagacca agcaatacaa
    40021 caaaatactg atttaattct tttttcagaa ttagcaattt gtggttatcc tccacgtgat
    40081 tttttatact ttaatcattt tatagaacaa tgtgagctta ttattaatcg catttgtgca
    40141 attgcacagg atagaatagc tgttgttatt ggtgctccaa gcaaaaatcc aattccagac
    40201 ggaaaagatt tatataattc tgcttatttt attgaaaatg gaaaagtaaa acatatcact
    40261 cataaaacat tattgccaac ttatgatgtt tttgatgaat atagatattt tgaatctaat
    40321 cgtacctttg aattggtaga atataaaggt aaaaaaattg ctatcaccat ctgtgaagat
    40381 atttgggata ctggagagac aaatccttta tatacagtca atccagttga agaattggca
    40441 aaattacaac ccgatttttt attgaatttg tcggcatcac catttaatta taaacaagca
    40501 ataaaaagaa ttgaaatagt aagccgtaat gcttcagctt ttaatattcc tattttttac
    40561 tgcaataata ctggagctca aacagaatta atttttgatg gtggaagttt ggtaattgat
    40621 aatcaaggtc gtttaattga tgaattgcct tattttagaa ctgcacaaaa atattatgaa
    40681 ttagatgtta ataataccat tcaaattatt aatgatattc aaaatagtaa tactaaagat
    40741 gaaatggcac ttatacacga tgctataatt tgtggtatta aagattattt tggaaaattg
    40801 ggtttgaaaa aagctattct aggattaagt ggaggtatag attcagcttt gaccttatat
    40861 tttgcggtta aggcattagg caagcaaaat gtattgagtg tgcttatgcc ttcacaatat
    40921 tctaccaatc attctgttga tgatagtttg aaattagtag aaaatttaga tacatcttat
    40981 aaaataattc ctattaaaga agcatttgat gttcttgata agatacttca accacatttt
    41041 gatggcaaac cgtttgatgt aactgaagag aatatgcagg caagaattag ggcaatttat
    41101 ttaatggctt tgagtaataa acatggttat gtattattaa ataccaccaa taaaagtgaa
    41161 gccgctgttg gttacggaac tttatacggt gatatgtgtg gcggtttagc tgtattagct
    41221 gatatttata aaactcaagt ttatgatttg tgtagatgga taaatcgcaa cgatgaaata
    41281 attcctaaaa atattattga gaaagcacca agtgcagaat tgcgtccaaa tcaaaaggat
    41341 tcggactctt tacctgatta tgaaatatta gatcgtgttt tatttgaata tattgaaaat
    41401 attaaaggac cagatgaaat tagacaaata ttaggttttg aagaggcatt aatcgataga
    41461 atattaaaat tggtcaatag agcagagttt aaacggcatc aaacaccacc cattttaaga
    41521 atttctccaa gagcttttgg tagcggaagg cgtgtgccaa ttgtaaataa atattgtcaa
    41581 taatacttta aatacttttg gaatgatttt aagaaaaatt tcatttctca tttttatatt
    41641 ataactttgt attatgagtt tagatgccaa tttgaataat gaaattaaaa atgcaatgct
    41701 tcaaaaagaa gaagcaaagt tgagaacact tagagccata aaatctgctt ttttattagc
    41761 aaaaactgaa aaaggaggaa atggtcaaat atcagaagaa caggaattga aagttttaca
    41821 aaagctattc aatcaaagga aagaatcttt cgagattttt accaaagaaa atagagcaga
    41881 attagcagca aaagaaaaag aagaaatgga tattatagga gcatatcttc ctgctcaatt
    41941 atctgatgaa gaattgaaat ctactattca acaaattata caaactttag gtgttacatc
    42001 tattaaagaa atgggcaaag tgatgggtgc tgcttctaaa caattgcaag gaaaagctga
    42061 aggtggtaga atttcttctg ttgtcaagga gttattagaa tagtattcta ttttcttttt
    42121 gtttactaat caaagcgaat tgctttaatg ggagagattt ttccaattat ccttgatggt
    42181 atatacaata ctaaaaagca aattacaaaa gtcaatatat tgacaactaa tattttggga
    42241 atatcaaaat aaacaggaac ttcggacaag taataattct cttcattaag tttgatgatg
    42301 cctgtatatt tttgaattaa acataaaatt attgccaatg aatttccaaa aagtaaacca
    42361 tagaaaataa tataagaagc ttgataaata aatatctttt ctatttttct ggtactgaca
    42421 cccaatgctt ttaaaatacc aatcatttgt gttctgtcta aaattaaaat tataagtgcc
    42481 gaaatcatat tcaacaatgc aaccacagcc ataattaata agataatata ttcattggtt
    42541 ttttgtaggt tcaaccattc aaaaatactg ggataaactt ctttaatggt ttcggcatat
    42601 tttgtgttgt caatgatagt gtagtagatt ttgtctttta tttcatccat ttttcccatc
    42661 tctttcaagc gaacttctaa tccagtgact tcattcggtt gccacatatt gatttgttgt
    42721 aataacctta aatcacataa aacaaacttt tcatcatatt catacaagcc agttttgtaa
    42781 atgccagaaa tatgtacttt tctaccaata ggttgagtcg tttgtttttt tagaaaatag
    42841 acaattatat tgtcatcaac tttcaggttg agtttgtttg ctaaaacttt tgaaattaat
    42901 acatctttac taaatgctgt atcattgtag tttatagctt ttccatcaac aatataatca
    42961 ttgataaatg tccaatcggt atgttggtca atgcctttta cagcaatacc ttcaatctct
    43021 gtttttgatt ttaatatacc tgcttttgtg atataaggcg aaactatttt tacaccagat
    43081 aaactcttta tcttattaat atttattgtt gaaacatcaa ttggttcagc ttcaaaagaa
    43141 cttttgttat ctaaattgtg aatgtgaatt tctccccaaa aaccaaatat tttctctttt
    43201 atttcacgag taaatccacc taccatacag atagaacaaa tcataatagt tacactcaat
    43261 gttatagcaa ttatggctat cctaatgata aacctagaga atgattgttc ttctccatga
    43321 gcaattcttt gtgctataat tctttcaata ctcaacgcat caaagataca taatatttgc
    43381 aggattatat accagctgca acgtgtgctg cttggttgat agcacgttta gcatctaatt
    43441 ctccagcctc ataagctcca ccaatcacat gtactgtttt gccaagagct aatagtggtt
    43501 gctctaattc tcgcaatgaa atttgtcctg cacaaactac tacattatca cattcaaaaa
    43561 cttgtgccat tttattttta tctataaaat gtaaaccttg ctcgtcaatt ttggtatatt
    43621 ctacacctgc aatcattttg accttcttca tttttaaagt ggctctatga atccaacctg
    43681 ttgttttgcc taattttgca ccaactttcc cagcagttct ttggaacatt acaacttctc
    43741 ttgctggtgg tgcaattact tgtggaatga taccaccacg agtttcattt gttctatcta
    43801 cgccccattc tttcaaccat tcttcacggt tgagtgtcaa actttcgcct tgatgtgtaa
    43861 gatattctga aacatcaaat ccaattccgc cagctccaat taccgcaact cttttaccca
    43921 ctttagcttt atttttcaag acatcaatgt atgataggac ttttggatga tcagcacctt
    43981 ctaaattaat atgtcgtggc gtaatgcctg ttgcaatggc tatttcatca aagtctttta
    44041 aatcatctgc attaacacgt ttgcctaatt ggacatcgac cttgtatttt ttcaaatgta
    44101 catcgtaata acgaatggtt tcgtaaaatt cctctttacc aggaattgtt tttgccatat
    44161 taaactgacc tccaatttct ttttcactat caaataaagt aacttgatgt ccacgctcag
    44221 cagccacttt tgcaaaagtc aagccagctg gtccagctcc aaccaccgca attttcttct
    44281 tattattggt tttggtataa ttcaattctg tttctgtaca agctcttgga tttactaagc
    44341 atgaagatag tttattagaa aatgtatggt ctaaacaagc ttggttacaa gcaatacatg
    44401 tattgatttc ttcttctttt ccgtcaatag ctttttgtac ccaattttca tcagccaaca
    44461 ttggtcgtgc catactgacc atatctgcac aaccatcagc caacactttt tctgccacac
    44521 ttggcatatt gattctgttg gaagtacaca aaggaatttt tacttcactt ttgagttttt
    44581 gagttaccca tgaaaatgca gctcgtggaa cagaggttgc tattgttggt actctcgctt
    44641 cgtgccaacc tattccagta ttgataatag ttgcaccagc tttttcaatg gcttttgcca
    44701 attgtacaat ttcttcccaa gaacttccat catcaatcaa atccaacatc gaaagtctgt
    44761 aaataataat aaaatttgga ccaaccttct ctcttacttt tctaacaatt tctactggaa
    44821 atcgcattct attttcataa cttccgccaa attcatcagt tctttgattc gtatgtttta
    44881 ctaaaaactg attgattaga taaccttcac tacccataat ttcaacgcca tcataaccag
    44941 cttcttttgc taatgcagct gaatctgcga agtcatttat cgttctcttt acgccccaag
    45001 ttgttaaagc tcttggttta aaaggattaa ttggtgcttg tattcttgaa ggtgcaacac
    45061 ttaatggatg ataagcatat cgaccagcat gtaaaatttg catagcaatt tttccaccag
    45121 cagcgtgtac agcatcggtt actattttat gtttttttac atgacttcta ttggtcattc
    45181 tttctgcaaa tggtttcaac caaccttccc aatttggagc gataccacct gtaacaatta
    45241 atccaactcc acctttggct ctttctgcat aaaatgttgc caatctttga aatccatctt
    45301 tagattcttc taaattggta tgcattgaac ccattataat tctgtttttc agcttagtaa
    45361 agcctaaatc taattcggat aataaaattg gatattgatt tgccataaaa tatgctatta
    45421 ataggtgcta atatagacaa aaatacaatg cacgcattga actttggata aaaaacttat
    45481 ttctgtttaa cttctaattt tacagtttcg attttggttt cagatacatt gaggatttta
    45541 aaaatatagt ttccaatctc aataatttca ttttgttttg gaattgtttc ataatgctca
    45601 ataataaaac cagatattgt atcataatcg ccttcaggta aatttaggtc aaattcctta
    45661 ttaatatagt caatttctaa tcgaccagaa agtatgtatt cattggcgga taattgattt
    45721 tcaatgaact catctttatc gtattcatca tcaatctctc caaaaatttc ttctaaaata
    45781 tcttctaacg taataacacc agcagttcca ccatattcat caacaaccca agctatactt
    45841 ttttggtctt taatgaaatc attcaatagt tttttaatat gtattgtttc aggaataatt
    45901 tttataggaa ttaatataga aagtatgtct tttggttttt tgtgcaaatc aaaatgatgt
    45961 atatagccta ttattttgtc tatactttca tcatacacta aaattcgtga atggtttgtt
    46021 ttgactataa tttcttttaa ggcatcaatt gttccagttt ttgatatggc acttatttca
    46081 cgacgaggta ccatacaatc tctagctttt aagtttttta aatctaaaac attttctaga
    46141 aattcttgat ctacattatt gtcatcatcg ttatcttctt cgatattatg ttcttttata
    46201 aaagaatctg tatctatccc agcataaaaa atagttgcgg tatcattttt aataccaaat
    46261 aaacttagcg taaattttat aagtgcttgt acaataactg aaataggttg taaaatccag
    46321 tgaagtacat tgaaaggata aataaaaatt ggaagtagat tgtcggaaaa taatttaaaa
    46381 atagatttag gaatataaac gcctaataat aaaaaaatac ttgtgttgat taaaattaga
    46441 atgaaaccag caagtgcaaa cggtacacca agaatatcta ttaaaggtag aaaatgaaaa
    46501 aaggaaacga tgctaaaaat gactaagagt aaataaattc ctaaggtaat tgtaatgtca
    46561 tattttgaag gatttttgat ataatcattt agaataatac cagtataatc tcctttttga
    46621 taacttaact caatttttaa tcggttggtt gtttgaaata ttgcttcgat tccagacaga
    46681 attgagacga taattattat gaatagtaga aaaagcattt gatggtctag aaaagtaaaa
    46741 ttacaaaaat atattaagat tatctaaatc gttcttcatc atcgtatgca actgttccaa
    46801 aaactctttt gatagaatat tggctaaaat cttctttggc aatcatgcca gagccacgta
    46861 ctatgtcgtg tggcgttttt attttgatat ggtttttagc aaaaatcatt ttggtattca
    46921 tatcccaaag caattcatct gtcgatagtt cttcgtgttt gtagttatac atatcgacat
    46981 ttcctttgat gaggattgtt cttaaattat catcataaga tgctgaatca gcagacaata
    47041 cagaactgag ttgcccagca tcataaaatc gaacttctat acctttctca aaaattaatt
    47101 tattatcacc ttttaaatat cgagatactg atttgccata tatttcagct tttaaggaag
    47161 caccttcttt ataagttaat tttacgctgt ctgcaacctc aatattagct tctttctcat
    47221 tgattaatgc atcaattttt gccatatcat tttcttgaca gctaactaaa attaataaaa
    47281 taaaaaagta aatcaatcgc attggagtaa agatattgat aataactcag aatttaaaca
    47341 agatttccac tacctatttt tagtgatgtc taaaaccaaa actgtatgta attttgtaag
    47401 gaataaatta acgaagaaaa ttgatgaaac gactatacta tttaggttta gttttaataa
    47461 cgacgatagc agtttttaca gcttgtagta aaataaaaat agagaaaccc aaattgaaaa
    47521 ataatctaat gacttgtgat agtattagaa aaggcggcgt atttgtagag attcctaatt
    47581 tagattttga agattggact ccatcaagtt ctaaaagata tgaagagccg tcattgactt
    47641 gtttttgggc aacaccatct aaagcaaatg atattatttc ggcaattcca attacggtat
    47701 caaaagtaac aggcgacgac gcccattctg gtaaatatgg ctgcaaaatt caatcacaag
    47761 cttggggtgc tttatttact tcaggtacag tagcatcagg aaaattttcg cctgatttta
    47821 aaaacccttt gcaatcaatt cagtttggcc aaccatttaa taagaaaatt aaagaagtaa
    47881 gaggttggta taaattccaa tctgtaaagc aagattcttg tagtttttat tgttatcagc
    47941 ttaaaaaagt gggtagccaa gtagatacta taaatttttc aagaattgtt acgaatgttt
    48001 ctcaacctaa ttggactgaa tttgtattaa cacctgtgta tagaaaagat gatgaaccta
    48061 acgtattagt tttgtacttt gcatcaagtg aagctggaag tgaactaaaa gggcaagttg
    48121 gaaatacatt aattattgac gatatttcta taacttatta ctaaatataa tcttatgaaa
    48181 aatatttttt gttttgttat attgacattt ggttttttta tagatggttt ttcagataca
    48241 atagttacta aaaagccttt ttatgaacca aaagtaaaaa tgagtaaaat ggattatggt
    48301 attaatgttg gaatgacttt agcatttcca ttaggtgtat tggtagaggg agcgaaaggt
    48361 atgcctttcc ctggaccatc gattggcatc aatacaagat actatttttc caataagttt
    48421 tcaatccaag caggtataaa ctattattgg aatcaaacta gatttgaaac tccttatgct
    48481 aatttttctt atgttggtgc aatagacatt tcaatgccag atggttctac agttcagaag
    48541 tttgatacgg taaatattta ttatgcagta gtgaaagatg gattgtttag caataaatat
    48601 ttaggatttc cgatacaagg taatttacat atcaataaag tttggagttt aaatgctggt
    48661 gcttatttgg ctattttgtt aaaaggcgga atgacaggta tggcaacaga tgtagtttta
    48721 ggtgatggct ccagttcaga ttttcaagtt tctgatgatg ttccttttga ccaaggcgat
    48781 cagtttgcaa aatttgatta tggaatgaac tttggaacaa atgcacaatt acccaatggg
    48841 tttaatttcg atttaaaagt aaatatgggt attgcttcaa tgtttaaaag aagttttaca
    48901 gcacctcctg gaatttatag aaatgttttt tttcaaggaa ctttaggtta tcgacttggt
    48961 gcaaaagaac gatttaaacc aaatttgcca aagatataaa aataaaaaga gcgttcattg
    49021 aacgctcttt ttattttatt ctattgcaca cttttaaatt gaaatatcta atatttctaa
    49081 ttccattgca ccagcaggaa tttgaaccat taccttttcg cctttacttt ttccaagcaa
    49141 tgctgcacca atcggagatt taactgagat tttcatttgt ttaacatctg cttcattttc
    49201 agaaactaat tgatatgtaa actctttttt tatttttaaa ttcttgactt ttactttaca
    49261 aagtacagaa gctttggatg tatcaatcgt gctggtatcc accactctag cgttaccaat
    49321 tgcggcttct aattttctaa tttttgattc taaatgacct tgagcttcct tcgcagcatc
    49381 atattctgca ttttcagaga ggtcgccctt ttctctagct tctgcaattg cttttgaggc
    49441 atttgggcgt tcaattgcaa cgagatgttg taattcttgt cgcattttct ctaagctttc
    49501 ttctgtaaca tatacaattt ctgacataaa tttagttttt tgctttaaaa aaataaaaca
    49561 cgttcttttt agaacgtgct taaacttata ttaacaaaga taaatagaat aagcttcaaa
    49621 agcaaatttt tttaatcttt ttaacagaat agaaatttag gtgcccagaa caggattcga
    49681 acctgcacag ccgtaaagcc actaccacct caaagtagca tgtctaccaa tttcatcatc
    49741 tgggcattgg ttgaatggta caaaaatagt tgtattattt taatcttgca aaataaaatg
    49801 aagtaattct tattggattt tattttgtgg aattcctact aaatttggga ctaatttttc
    49861 aagccaagtg tttatgtgat tcttaaaaag tgtacatttc gtttttgcga ttatattaac
    49921 tctccttttt tattattttg atatccattc tcttattttt tgttgtattt caggatattg
    49981 ttttttatta ggcatatgtt tgttattttc gagaatatat atatcctgaa gatttttaaa
    50041 tatattttgt gcttttttta ttgattcgtt tccaggaaaa aatatatcgt tatcaacggt
    50101 cattaaataa actggttttt caaaatgcat aacgtcttta atttttaaaa ttggaggtct
    50161 tctataatcc atttttacac cgagtaacat cgccttttga agcctaaaca taaaattatc
    50221 atcctcatta ataaatttac ttagaaaatt atgtagatgg ttatcatttt ttgttaatag
    50281 aaattttatt agaggaaaag ttactttggt taatgatgga aaaaatgcac catctactaa
    50341 gcctgcagga acaacaaaga tacatttgga aactctctct ggtttgtgtg aaattagttt
    50401 ttgtaagata aacccaccat aagaaacacc aataaaattt gctgttttta tttgtaattt
    50461 ttctagtact tcatctgccc acagagcaaa agaattatct ttaatattta atcttgtttc
    50521 ttcactctga gttgcttgtc ctatagtatc tattgcaaat atattatatg aatcagtaag
    50581 ttctttaatg gcttcaagag caacaggagc accagtattc ataccatgaa acacaattat
    50641 cttagttcca tttgatttcc ctgttttaat tactcttgtt cttccatatt ttgtgtttac
    50701 atcgatttct tgatactcta tttttaaact atttaatttc tcataataca attcttgtat
    50761 tgcaatttta gtttctttat ttttgtagat agacatttta aaatatttta tacatcaaag
    50821 ttaaagtaaa aaaaacaatt taggctattt ggactaaatt taatattatg ataaaaaata
    50881 ggattaaaga taaaggcaga gaattattta atgaattcgg ttttcggaat attacactgc
    50941 gtgatatcgc aatttctatt aataaaagtt atggaaatat aacctatcat ttccctacca
    51001 aagaacattt gctacaagct attttttctg atatgaatga tagactgatt gccttacaaa
    51061 gtaatttgcc taaggacatt aattatttag agtattttct attattgcct tattatagtt
    51121 ttgatatttc agtagattat ctattctttt ttaaagattt tgtagaaata aaaagagcat
    51181 atccaaaatt ttatgagata gtagaaaaga ctaataattt tagaaaagaa aaatggaaag
    51241 acttatttat attgcttcaa caacagagtt atttagaaaa tactttgtct gaacaagatt
    51301 tgttttatat tatggaattg agtacaggaa ttagaatatt ttattttatg gaaaatgatc
    51361 tagctaattt aaataaacag atttttgttc aaaaagttaa taaattatta tacccttatc
    51421 ttacaaaaaa agggaaatca atctatcaaa tgaacaagta actatttctt ttgttatttt
    51481 ttaaaaaatt ttctgacact aattatatcg ctaaaaggca aattgtttga aatgcttgtc
    51541 tgttatgcta taattattat aatttgagtt tcaattgcct tcatagaaaa ttgtttttga
    51601 agaaattact ttctcattat aaatagataa agtcatatag atataattag acttatattt
    51661 attttggtat aattttttct atctttattt cgtgcgaaat gtactattta taattttttt
    51721 tattggaacg attacgctct ttgcaaatcc attattaata ggagattgga atggatttat
    51781 agtgcaaaaa tatgaaacag ataacccaat ttttttacct tgtacattga atattagttc
    51841 ggtttcatcg agtaatcaat ttgctggaac tatagaaatc tcattcaaac atgcaaatgg
    51901 aaaagtttat aaaagtaagg caactgtttc tggaatttat gatacaaaaa cttataaaat
    51961 tagtattgta gatgagcaat ttgtctatca agatttatta cccgaaaaag cgaaatggtg
    52021 cttaggtatc acaaaaggtg gtttttatag aagtaaaaca gctaaacaat atttgattaa
    52081 aggactttat tcaaccaaat gcagtgcaga tgttggctta ttggtggtgt ataaaaaata
    52141 aaatataaaa gatgagtaaa tggtttataa aggcgttatt acaacgttca ttctcaattt
    52201 caaaaaatcc tgaaaagatt aatcgccttt ttcaaagata tcttacagga aaagataagt
    52261 tgaatgaaga aaaatttggg tataaaattt tacatgcaag tgatcatatc gcttatttta
    52321 aaaaacatag taataagtca ttggcagatg taaactgttt agaattaggt acagggcaat
    52381 ttccaatagt gcctattttt tatttcctaa atggtatgaa tcagattaca tctatcgatg
    52441 ttgttagttt tataacgaaa gatagaattg aagatacttt gaatttcttt attgaataca
    52501 gaaagaaatt tccagaaaaa ttcagtgaaa ttttagaaga aagatggaat atccttgcgg
    52561 atactttagc caattttgct caacaagatg acatgcacaa aatattgaaa aattttggaa
    52621 tagaaattta tattgctgat gcaagaaaaa ccaaatatcc tgaaagtact tttgatttta
    52681 taagttctaa caatactttt gagcatattt atcctcaaat attagaagga atattgaaag
    52741 aatttcagcg aatattgaaa cctgaaggta taatgtctca ctttattgac atgactgatc
    52801 attttgctca ttttgacaat aaaatcaatg tttataattt tttgaagttc agtgataagt
    52861 tttggcaccg aatagacaat gaaatattac cacaaagccg tttgcgtttt aaagattatc
    52921 tagcaatcta tcaaagcttg aatattccga ttattgacca aaaaaattgg gattatgatg
    52981 tgagtttaat tgattctatc caactgaata aaaaatatca aaactataca aaagaagaat
    53041 tagcgattgt tcacgtttat gttgtttctt ccaaatagtt tttgatgtaa attgtatatg
    53101 ccataagctg ctaaaaatat atagaacggc ataaatggat ttctaaatct tggagttgga
    53161 taaatacttg acaacgaaac ataatatcca atattcaaaa tacagaagat taaaatataa
    53221 aaatctttat ttttaatggt atagccaata ccaactaagg tatagccata tacaatgact
    53281 aataaactca aaataaaaat tcccaatgct atttctgtag ttgttttagt tgcaaaaaac
    53341 ttttttgcag ccgcaaataa gcccaatcca actttatctt tgatatccca tcttttggtt
    53401 tcttgatgta aacgcaacat atattgttcc gttcccatat tgacgtgtaa atttatagca
    53461 ccttgtattt gtgctagaat aaattctttt ttgtgcgata gaaggtaatt tttagcaact
    53521 ttcgcaaata tatctgtatt ttcaaaactt tgccttctat tcagttcagc aaaatttgaa
    53581 ggcatactgt cttttaaatt tgtaggtgca gctgctttta attgatatag aaaatgttgg
    53641 atgatagtat ctcttggtaa ttttgaattt tgaaactctg tataaaaact acttaaaatc
    53701 aaagcattgt gatccattgt attagaaagt ttaaatctgc cataatagat ataattccgc
    53761 attgcccacg gaaaaatact tagcaaatag aatacagtta ataatacagc aaatttcagt
    53821 tttgaagaat tgttttttag agtataaaaa attaccaaca aaacataaac gataattaaa
    53881 tattgaccag ctggtcgtgt gagtgttgcc aaacctaaac taataccagc taatataaaa
    53941 tataaaattt tattttgctt taatcctcca atgataagat acaaactgaa cattgtaaaa
    54001 aacataaaca acacttctga gtacaaaaaa gtcgtatata atatcagtgt cggttctatc
    54061 gcaaagataa atagggcaat attagcaatc attctattgc ttaattgagc aataatcttg
    54121 tagaaaaaat acccagtagc aacacttata aaaatttgtg caaacagaac aataaatggc
    54181 ttgactccaa aaagtgcata aaaaacagca ataaaagcag gataaatagg cgttctcaga
    54241 atttcattct ttattctgaa agtttctaaa agaattaaag ctagtttgtg gtagccaaca
    54301 gcatcgtaaa gtaatacgac tttatcttgt atttctggca accatggttt aaagatgaaa
    54361 agcaataaca agcgacatac aaacactaat atcaataaga agtatagtgt tttgtttttt
    54421 tgtaactgtt tcattactcc aaatatacgt tgtttattgg aataattgat gatgattaaa
    54481 agtatctgag aacgaatgct ttgatttact tgctcaagaa cattgatttt tccttataca
    54541 attgtctgaa ataaggatca ctcaaactct taataaaata aatagattct ccagttgatt
    54601 tcatttcagg acctaattcc ttatctacat ttggaaattt atcgaatgag aaaacaggtt
    54661 ctttgatagc aaacccttct aatttcggtt ctaatttaaa atcttttaat ttgtgtgtgc
    54721 ctaacataat ttttgttgcc acattcaaaa atgaaacgtt gtaagcttta caaataaaag
    54781 gagttgttct tgatgcacga ggattggctt caattacata aactttatcg ccttttattg
    54841 caaattgtat attgattaag ccttttatat ttaatgcctt acagattttt tctgtatatt
    54901 ctatcatcgt ttccacaatc attggaccta aagaatacgg aggtaaaacg ctgttgctat
    54961 cgccactatg aatacctgct ggttcaatat gttccattac acctaaaatc tgcatttgtt
    55021 cgccatcaaa tatagcatca atttctgctt cttttgctct atctaaaaag tggtcaataa
    55081 gaatcacatt atctggaata tctctcaata gattaatgac tgcagtttca cattcttcat
    55141 cattgataac tatacgcatt ctttgtccac ccaaaacata gcttggacgt actaaaacag
    55201 gataaccaac tcgatttgca acttccaaag cctcatcagc attgatagct gtgccataat
    55261 caggatatgg aatccctatt tcttttaata aatctgaaaa tcggcctcta tcttcagcag
    55321 tatccatatc ttcaaatgaa gtgccaatga tttttatgcc tttatcgtgt aattttttag
    55381 ataattttaa tgctgtttgt ccacctaatt gtacaattac gccttctggt ttctcgtgtt
    55441 cgataatttc ccaaagatgt tcccaatata caggctcaaa atataattta tcagcaatgt
    55501 caaaatctgt agaaactgtt tctggattgc aattgaccat aatagcttca tagccaactt
    55561 ctttaattgc caataagcca tgtacacaac aataatcaaa ttcaatacct tgcccaattc
    55621 ggtttggccc ggcacccaaa actataattt tcttttttga agatacctcc gattcgttgt
    55681 gttttactaa tgtcatgatt gtacaatttc taacacacga aggtacagct ttttttgttg
    55741 cttttatttt aagaatttat tagataactg ctccttagct gaattaaaac cttaaaggtc
    55801 tgtcattata aatattcagc atcaaaatgt aatggcagta attcttttat agaattaaat
    55861 ataaaggttg tgccattatt tccatgtaat aaaattcgga tattgcttct ttgcttagat
    55921 tcggtttcta ataaagtttg tctgcatata ccacaaggag caacaggaaa tggaacttcc
    55981 atattttgac tgcttgccac aattgctata gtttttactt tttctgtcgg aaataatgaa
    56041 acactagcac ttatggcagt acgctccgca cataaaccga ttgggtaggc tgcattttct
    56101 tgattgctac cttgaacaat ttttccgttt gccattaaca aagcagcacc aacattaaaa
    56161 ttggagtagg gtgcatgact accttctttt gctaatttgg cttgttcaat taatttttgt
    56221 tcttgttcgt ccaattctgt tattgtttca gcaacaacat attttatagg tataagtttt
    56281 tctgtcatag tttaatttcg cttttttcta ttaatacttt tagtttgagt aagccaacca
    56341 aagcaatggc atcttttatt ttgccagaga tagcccaatc tattgcttca gttattggta
    56401 gcttttttat ttggatttcc tcagtttcgt catagtttgt attggttggt attaaatctt
    56461 gtgctacaaa tattattcct atttcatcag taacagaatt ggacgtattt aattcagcaa
    56521 tctttgtcca ttgattagca taaaatccaa cttcctcttg caattccctt ttcgctgatt
    56581 ctaatatatc catatctaat ggcccaccac cttcaggaat ttcccaacta tatgtattca
    56641 gagcatatcg atattgtcca actaaataag tatagccatc tttgtcaatt ggaataatgc
    56701 caattgcata atttttaaaa tgtactgtcc cataaatacc tttcgtttga ctgggcgtaa
    56761 taatattggc atgtttaaca cttatccatt ttgtatcata tacaatagtt tcatcaatag
    56821 tttgaaatgg atttcttgtc tcgtctatca ttttttagga atttcttgtt aatattctcg
    56881 tttacatcag ttcgtcaaac aatagttgca taccgatact ggctttttct gtaatataat
    56941 ttatgttttc tgtgtagcta acacttggtc tgttagggtc tatgacatat tttggaacag
    57001 taaaatcagt ataatgtatc aaaccagctg ctggataaac aactaatgat gttccaatta
    57061 caataaatat atcagcacgc ttacaaattt ctgaagcttt ttctatcatt gggacttgtt
    57121 ctccaaacca aacaatatgt ggccgtaatt ggtcaccttt ttcgcaatta tcaccaatat
    57181 tcaaatcttt tttccaagga taaaccaatc tttcatcttt agtacttctc acttttaata
    57241 actcgccatg taaatgtatt acttttgatg aaccagcacg ctcatgcaaa tcatcaacat
    57301 tctgtgtaat aatttctaca tcatatttag attctaattt agctaaaagc tcatgagcaa
    57361 tattaggttg gcattctaat aattgttttc gtcgttcgtt ataaaatgct aataccaatt
    57421 ctttatttct agtccaagcc tcaaatgttg caacatcttc aattcgatga ttttcccata
    57481 aaccatcact atctcgaaaa gtttttatgc cactttctgc acttatacca gctccagaca
    57541 atacaactat ctttttcatt aatactaata tacaaaaata atctaagacc gttgcaaaag
    57601 tcttaatcct tatatttaat tattatttac tgaaaattcc tcctttaaat acttaccagt
    57661 aatgcttttt ttgttttgag ctattgcctc tggtgtgcct tctgcaataa tttctccgcc
    57721 accgctaccg ccttctggtc ccatatctat aatataatcg gttactttaa gcacatcaat
    57781 attgtgttca atgacaacta cagtattacc tttatttact attgtctgca atacctctaa
    57841 taaaatcttg atgtctttaa aatgcaaacc agtggtaggt tcatctaata tataaaatgt
    57901 attgcctgta tctcgcttac taagttcttc tgatagtttt actcgctgag cttccccacc
    57961 acttaatgtc gtggaggatt ggcctaaacg aatataaccc aaaccaactt catctaatgt
    58021 ttttagcttt ctatagattt gaggaatatg ctcaaaaaag cttactgcct caacaattgg
    58081 catttctaaa acatcataaa tagatttgcc tttatatttt acttctaatg tttctctatt
    58141 ataacgtttt cctaagcatc gtggacattc cacatataca tcaggcaaaa aattcatttc
    58201 aat
//
LOCUS       DAOQCW010000003        54592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_203_length_54592_cov_5.917927, whole genome shotgun sequence.
ACCESSION   DAOQCW010000003 DAOQCW010000000
VERSION     DAOQCW010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 54592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 54592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54592
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203_length_54592_cov_5.917927"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PLS16_00595"
     CDS             complement(<1..531)
                     /locus_tag="PLS16_00595"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNJ59602.1"
                     /translation="MAAERTKEYLPLLQNKKVGLVVNQTSVVGNTHLLDTLIALKINV
                     VKVFAPEHGFRGTADAGESVKSGIDAKTKTPIISLYGNNKKPKPEQLVGVDILLFDIQ
                     DVGVRFYTYISTLEYVMEACAENNKSLIVLDRPNPNGHYVDGPILETTQKSFIGMQKI
                     PIVYGMTIGEYAQMLNG"
     gene            complement(683..1069)
                     /locus_tag="PLS16_00600"
     CDS             complement(683..1069)
                     /locus_tag="PLS16_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNJ59603.1"
                     /translation="MSTRVNYLEGSEWETKIGYCRAVKIGNTIEIAGTTATVNGEVVG
                     KGDMYLQTKTILNKFEKVLSELGASYKDVVRTRIFCTDISRWEEIGKAHGEIFAEIKP
                     TTAMYEIPKLINPDLLIEIEATAIIE"
     gene            complement(1159..2664)
                     /locus_tag="PLS16_00605"
     CDS             complement(1159..2664)
                     /locus_tag="PLS16_00605"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNJ59604.1"
                     /translation="MIKQLVSIFTLGLLINTTNLIAQSDVDALRLGMQYSNGSARNMG
                     LGNTMGSIGGELSSIHTNPAALGRITSSEVSLTPVVSIFNSNSNYIGKITEDSKLKFG
                     VYNFSAAMVSKYVSNYGKWSTPKVGFSFNNLANLNQNFYISGFNQHNSLVDKYFELLD
                     EHASTTNDLENMFPFNASLAYNTDLVYFDTTDHRIYSAINNGNVQQDIVIQRKGNLNE
                     MAIAVASTYSGEKRKIHIGATIGVPTLNFTESTIHKETDTPDSSYNFNFFKQNSSFRV
                     SGLGVNLKLGLLAELSKNVRLSFAFQTPSVLFLNDAFNSKINADYVTYTAEAESPEGL
                     MKYNLRLPWKLNTGMSYISKIGLIAAEYELSDAGASKYNYKNADADIKEEQRALNQTI
                     KNKYKLYHTLKLGLEAKIDPIRLRGGIQIQSSPFDKNFVTDQSGQLNFIYSAGVGYRK
                     NKFAIDLAYQFTKGKESYTPYVLQNEAPQFQYTSENNFKRSVIALTLGYKF"
     gene            complement(2684..3703)
                     /locus_tag="PLS16_00610"
     CDS             complement(2684..3703)
                     /locus_tag="PLS16_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59605.1"
                     /translation="MKRFILNMFLVLPLAINSTYADDVYDDFTPTEKKIKTEKTYSPS
                     SSGSQYYNNNSYQYDNYNNDDYSSNSYNNENSNYYEEDGYDSYNNNGNSRYADRIRRF
                     HNPSVRFSVNFNACRPSYYGYNGYNSYYNDWMWNSGSSFYPNWYGGNTQIIIMNNYNP
                     WNRWNSWSNPWYGFNHWNSWNSWNPWSWGNRYNSWNSWGPYGYDNYYGNNYYGNYWNN
                     NYYPSVNKPSKPAPTYTPRPNISTNKPSYNNNSYPENNNKNNNNENYQINKKTNSNYS
                     KPQYQPIEKGDNNNYKPQYKNNNSGGDNYKQSPSMNNNNNNYKTPSPSNKTTPSGIKV
                     TKTPK"
     gene            3850..5331
                     /gene="proS"
                     /locus_tag="PLS16_00615"
     CDS             3850..5331
                     /gene="proS"
                     /locus_tag="PLS16_00615"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNJ59606.1"
                     /translation="MSKGITSRAENYSEWYNDLVLKGGLADYSAVRGCMVIKPYGFTL
                     WENMRDILDKKFKETGHVNAYFPLFVPKSLFEAEETNAEGFAKECAVVTHYRLKTDPE
                     NKGKLIIDPEAKLEEELVVRPTSEAIIWNTYKKWIQSYRDLPILVNQWANVVRWEMRT
                     RLFLRTAEFLWQEGHTAHATKSEAVEETVQMINVYADFVENYMAIPVIKGVKSANERF
                     AGAEDTYTIEALMQDGKALQSGTSHFLGQNFAKAFDVKFVNKENNEEYVWATSWGVST
                     RLIGALIMAHSDDEGLIIPPKIAPHQVVIIPFLSKNVEETQSMIDKANELKQNLSKLG
                     IRSIVDSDETKRPGFKFAEYELKGIPLRVVIGPRDLANGVVEIARRDTKEKSTFPIES
                     LQETIVGLLDEIQNNLFAKAKRFRDSHITEVDDFETFQKVLEEKGGFISAHWDGTPET
                     EDKIKELTKATIRCIPLGNKKEEGKCVFTGKSSQERVLFARAY"
     gene            5536..6309
                     /locus_tag="PLS16_00620"
     CDS             5536..6309
                     /locus_tag="PLS16_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59607.1"
                     /translation="MKTDQIDKFREALDALVNDFNIKVEEFRINLQNFSSDVQVQVQG
                     QRENLHEYGEKLKSRITNIVDINKIDKVREDLLAEAENVMDDAKQRLDDFFKVITDKV
                     NAGEKKAKEAANNVAKQAKAATESVEKEIKKAAKDVESKVKKAEKEIEKTAKEVEAKV
                     KKAEKNIEKEVKQTVAKAEKEVKANVSKVAKKVEKVAEEVQKNVATKKAPAKKAAAPV
                     AKAPAKKVAPTKATPAKAPAKKATAPAAKAPAKKAASKK"
     gene            6450..7859
                     /gene="asnS"
                     /locus_tag="PLS16_00625"
     CDS             6450..7859
                     /gene="asnS"
                     /locus_tag="PLS16_00625"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNJ59608.1"
                     /translation="MNIKQLLEQKSIGTQATISAWVRTKRQSKNVAFINLNDGSTIHN
                     LQVVAETIEEAILAKINTGACIKVEGNIVESSGQQAIELNAEKITILGEADPEKYPLQ
                     PKKHSLEFLREIAHLRPRTQTFSAIFRIRHALAFAIHQYFNDNGFFYLHTPIITASDA
                     EGAGEMFRVTTLDLNKLPKDENNNINYKEDFFGKETNLTVSGQLEGELGAMALSKIYT
                     FGPTFRAENSNTSRHLAEFWMIEPEVAFYELEDNMALAEDFLKYVIAYALKNCKDDLE
                     FLEKRLLEEEKSKPQNERSELALLDKLNFVVSNDFEKLTYTNAVEILKNAKKKFQYPV
                     DWGTDLQSEHERYLVEKHFQKPVILTDYPTEIKAFYMKQSEDGKTVRAMDVLFPGIGE
                     IIGGSQREDNFEKLKNRAAQVGIHAEQIWWYLETRQFGACPHAGFGLGFERLVLFVTG
                     MTNIRDVIPFPRTPKSAEF"
     gene            7869..8477
                     /locus_tag="PLS16_00630"
     CDS             7869..8477
                     /locus_tag="PLS16_00630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNJ59609.1"
                     /translation="MEKISHKKIIFDLGAVIIDLKSESDWWKEDLFPNFNQEQLEKLF
                     KEGFFHDFETGKISTTDFVKVMESIKINPDIAVEKAWNGILKTIPKHRIDLLNCLKEN
                     NQVYLLSNTNTIHTKTIHESLEFDFGMNIFDTIFDIQFYSQLVGMRKPNTEIYEHVQQ
                     TIGDSNSANFIFLDDKPENLIAPKKLGWQTILVDRDISELIA"
     gene            8525..9778
                     /locus_tag="PLS16_00635"
     CDS             8525..9778
                     /locus_tag="PLS16_00635"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNJ59610.1"
                     /translation="MKFSDYRINEDIKRNLEQLGFKKPTDIQYKAIPSILKGEDVLAI
                     AQTGTGKTAAFAIPIIHLIYEQKKSSRSDAIKCIIMVPTHELAIQITEVFEKIAYKTR
                     VKTLCIYGGVDQAPQIEALYKGVDIVIATPGRVFDLVHQGALALNKINYLILDEADHM
                     LDLGFIKDIQDMIRFLPAKRQTLFFSATINETIKKLAYSLVHNPIRIQISPKDPVSKN
                     VQHAVAFIEMDAKRYFLERVISEHKESKILVFVRTKVRAERVAKAMERVGIQTLTMHS
                     DKSQEERFSTLKEFKDGKVKVLIATDINARGIDIPNVDYVVNYDLPEKSENYVHRVGR
                     TGRGVQKGQAISFCSSEEKEILKEIETFLHNPIHVIDMEQAEQEEILFLAKEKEIPEG
                     DWRSLMAKAEEEEQLYKEKKKKKRK"
     gene            9814..10062
                     /gene="purS"
                     /locus_tag="PLS16_00640"
     CDS             9814..10062
                     /gene="purS"
                     /locus_tag="PLS16_00640"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HNJ59611.1"
                     /translation="MQYQVEINVMPLKELLDPQGKAVEQSLNNLNIHALNVRVGKRIE
                     LKIDATSKEEAFELTEQACKKLLYNPVMEFYKITVSEL"
     gene            complement(10077..12458)
                     /locus_tag="PLS16_00645"
     CDS             complement(10077..12458)
                     /locus_tag="PLS16_00645"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNJ59612.1"
                     /translation="MHFFPTNTPEKINFNTVIDLLSNKCIGSLGKNHIQNTGFITEHS
                     KLETELKIVHQIKSWIEEGLQIPHQGFNSMSFLNKIDIENYIIQVSEFIQLYNALKTV
                     KQLKNTAKKIDSERYTALTEIINQVPELDAVLLEIEKTIIIEQEIINESVSKILQQIR
                     KQKSQIQQQIQQNFKRVLTHYKAKDYLHDTEESIRNERKVLAVLSEKKRSVKGIFVDE
                     SSNGSITFIEPEETVHLNNELISCILEEQKEIEKILINLSNKIRPELDAIQTTQEFLA
                     YFDAQQAKANLAIEYQCTLPEIRKEKVIYLKNFRNLALEYHLRKHKHQIIPNTLHLDE
                     QNRILVISGPNAGGKSVVLKSLGLMQTMLQFGLLLPVDEGSIACVFNQIFSDIGDEQS
                     FENDVSTYSAHLQKMKYFVQKSNKDTLILLDEMGVGTDPSLGGAMAEAVLEILHHKNV
                     FGCVTTHFNNLKVYAAQTKGMQSAAMAFDKVALQPKYELQIGQPGSSFTFEIAQKIGM
                     PQNVIQLAKQKTAHNQKALDETLNEVQIEKHYIKGLRKNVQQKETQLNTIVKDYEQLK
                     EEFEKNKKRLTKSHEQKLLDFYNEQSRLLEKQMREWKESQQKKEDYTELRKNIDEQRK
                     TIEQKQQKEISNFQQKKDGIIQVGSKVRLEEGTEIGEVISIDNKKAIVAFGSMRTTFK
                     LHQLVLVEEKANTKKEFRLQKSSKQIIEKSKFESEIDLRGKLVEEAHNALETFMDQSI
                     MYGIHHLKIIHGRGTGKLRTYIHQYLKNYPYIKNYYFEKEEFGGNGVTLVELR"
     gene            complement(12486..14339)
                     /locus_tag="PLS16_00650"
     CDS             complement(12486..14339)
                     /locus_tag="PLS16_00650"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNJ59613.1"
                     /translation="MRKIKLLLIIITLAIVSLNYETYHTHFIQQTKSYLSASSKDLQV
                     SLDKIITEINAQDSIIDDKSTIIPLFKQARFHFKKIEFIADYNAEDIFKIPLVLDEKR
                     NFIIEAFAFHVLIDIEAMLAEDKIEKQAFKNLIKEFQYRNLKFYEIVQSELISESTIL
                     EALKYNIIKIETLNITDFDCETTRNSIQDIQANFLSIDTCLALLAPQTKSNVQEIINQ
                     TRLVIKSANNYLEQKDFETLERLYFTKQYLHPLNALFSQILKDSEIPLNEAIYNIIRA
                     VNLYSDNIYNKDFLYTPFYSVNKYKIPNQTEIELGKHLFFDKRLSKDGKMSCATCHQP
                     ELFFTDGLATSLTNQVGIFQDRNTPTILNSVYQRNLFYDLRAKNLEHQASQVVENHQE
                     FNNNYDTIVERLKNDKYYNETFKQLYPSSENYINAATINAAIAAFEHQLTALDSKFDK
                     YMRNESPIIDKEIENGYNLFMGKAKCGTCHFAPTFFGTVPPFYSESESEVLGVPVNWD
                     TLNYIQNSDVGRYKLLKFDFFKNSFKTSTIRNAEYTAPYMHNGSFKTLEDVLDFYNRG
                     GGAAFISIPNQTLSDQSLNLTSKETKDIISFINALTDSTWNTLKAPVSPMK"
     gene            complement(14451..14993)
                     /locus_tag="PLS16_00655"
     CDS             complement(14451..14993)
                     /locus_tag="PLS16_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59614.1"
                     /translation="MQGNDVYVAGYEWNGSKFIAKLWKNGVAQNLTDGTHFAYPKSVF
                     VQGNDVYVVGYESNGSKDIAKLWKNGFTQNLSDCTKDAYSRSVFVQGSDIYVAGYEGD
                     GSRYVAKLWKNGIAQNLTDGTHNAYAFSTYVLENDVYVVGYEDNGSKYVAKLWKNGVP
                     QNQTDGTNFAYAKSVFVHKH"
     gene            complement(15394..16389)
                     /locus_tag="PLS16_00660"
     CDS             complement(15394..16389)
                     /locus_tag="PLS16_00660"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNJ59615.1"
                     /translation="MKLRNRFCLLTLLIICFTFVACKTNTTEQAEVLVATSKLEKPSF
                     SSDSAYSFIEQQIDFGARIPNTPAQKKCADYLSNKLKQYGAEVIVQTTKLPIYNGQIV
                     PCYNIIGSFNPTIKRRLLLCAHWDTRPFSDRADKNQSTPNLGVDDGASGVAVLLEIAR
                     QLQIKPSEIGIDIIFFDVEDYGPPASEEAKYSGRDFYALGTQYWCKNPHIANYSASNG
                     ILLDMVGARNATFTFEGVSMQYAPEFMKQVWNNAASLGHGKYFIKRVTQPIIDDHYYI
                     NTLTTTPTIDIIYRTLETESGFAKHWHTHDDTIDNIDKNTLQTVGETVLATLYNY"
     gene            16512..17375
                     /locus_tag="PLS16_00665"
     CDS             16512..17375
                     /locus_tag="PLS16_00665"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNJ59616.1"
                     /translation="MSELKDNKWKQWWQKLKNVYRFQVVEEKTYDVKFVLELNRLNVI
                     TVSGILIAILTLLNFFFIAYTPLKQYIPGYGTVDSRKEIVDLKLKTEEVEDKIDANEK
                     YNKNLKNILTDKIEVKSLEDNITPVVVDPDVLVQISPDEHKLIKDIEKGLTNAELYDN
                     LQYKKTSNTLAALKAVPPINASIVSKFKNSKNAFTTIYKTPNASAIIATIDGYIVSID
                     EQKNGDFTVIIQHQGDLVSKYRNIKQVSKKSTNFVESGEEIGTADTQNNLEYELWYKG
                     IPINVEKYYKK"
     gene            17379..17777
                     /locus_tag="PLS16_00670"
     CDS             17379..17777
                     /locus_tag="PLS16_00670"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNJ59617.1"
                     /translation="MFNSKNSNNNASSLVANHINDNTNINGDIQANSDMRIDGNVKGN
                     IVCTSKVVIGRTSRVEGNIKCSDLTVEGTVIGDIIAEENLHFKSTAVFEGNIKYNTLI
                     VENGANILGSLLHNKKSNTPISKDATEKIG"
     gene            17755..17979
                     /locus_tag="PLS16_00675"
     CDS             17755..17979
                     /locus_tag="PLS16_00675"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNJ59618.1"
                     /translation="MQLKKSDKNQGLRQQLLAFSGMAFELLITIGIFTFIGIQIDKRI
                     ALKLPVATVVMCLFGLIVGFYFIFKQLKKL"
     gene            18001..18354
                     /locus_tag="PLS16_00680"
     CDS             18001..18354
                     /locus_tag="PLS16_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59619.1"
                     /translation="MRKWLLTLVILMLIGLVPAFIQTTLQKTWIYFDTVNFFIAFIFW
                     VIIYLAEQQTAQKKMGYTLGAIGIKFILTIVAVIGYFVVFKEKTTPEIMIVFIITSIY
                     YIIGYFFLYRLTNLK"
     gene            18430..19095
                     /gene="mtgA"
                     /locus_tag="PLS16_00685"
     CDS             18430..19095
                     /gene="mtgA"
                     /locus_tag="PLS16_00685"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HNJ59620.1"
                     /translation="MTRKIFRIFKRAIYLFFVLSILTVILFRFAPIPITPLMLIRSGE
                     FLLEGKSPILRYKWRSSDKISNNLKRAVVAAEDQKFYDHYGFDLEAIKKAAKNNEKGR
                     KIKGGSTISQQCAKNVFLWQGRNWIRKGLEVYFTALIELFWSKERIMTVYLNVIEMGI
                     GVYGAEAASEYYFNKSATSLTKSDAALMAAILPNPRKWDVKNPSGYIQRRQRWILRQM
                     NYQ"
     gene            19148..19534
                     /locus_tag="PLS16_00690"
     CDS             19148..19534
                     /locus_tag="PLS16_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59621.1"
                     /translation="MKIVKLLFIVILCSSTFVGFSGNIILYRKNGDVIPKETSQSWNT
                     NADKKVFVYYNNEDKYPFNTTYKISISKRVGNIFIRTEGKEFPVQKGQVESITFFELK
                     EPGDYILSVLDEQGYILSEQFYTVQK"
     gene            19590..20744
                     /locus_tag="PLS16_00695"
     CDS             19590..20744
                     /locus_tag="PLS16_00695"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNJ59622.1"
                     /translation="MVFIDSSVFYTRAKTHLKFVYLHCIKHFTIKNFFIGIAYGLIIG
                     VIVAFVNVLFRLIDELMFPNYKDIEVKQPVFIISNPRSGTTFLHRLMCLDEEKFVYNL
                     LYHTILPSITLFRMIDFFGKIDKKIGRPMRRFFDYIDNKMFSAWDDIHATGFNKTEED
                     EGLHFVSGISPSIGLITPHLKDFKELYIPDKLDDKANEQMKIFYKATIQRWMYALGYD
                     KMFLCKTVMSTGRLQMLHDIFPDVKIVFLLRNPYEAIPSFTSMFAEPWNTFYPEIPEN
                     SSEYREWGEVGIAYYKYFYEQKQNFKQENLITIPYTDLIKNPKDTVLKIYNQLQLEVT
                     PTFLEKLTAETQKSRKYSSKHEYSLEQYGFEKEHIYKELPMIFDELHLEK"
     gene            20945..23059
                     /locus_tag="PLS16_00700"
     CDS             20945..23059
                     /locus_tag="PLS16_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59623.1"
                     /translation="MQWRLYNKEIIWAFLVGFFVLFLFYVLNIEIFYPIGKNILDGSG
                     DGLKNLYTFGYYLKYGKGLFFNGMLYPFGEHITYVDAQPLYVWLIWLVESIFHFKIEN
                     PIIYIHLIVLLNIYFATFFVFLILKHFKVSFFVAFVGTVVIVLLSPQIYRNGDHFALA
                     SVVVIPFFWWWRLQITTSNTNYFFYAVTLSLVGFIHPYLLFMVVLLLLSYEFVLLLFY
                     KNKRWHFVFPILAFLLFYGIILYTDTITDRPKAAWGAKAFSCNWYDLLLPINGVVKDF
                     FIRKIPSIIPFYTEGHAYITVFGIFILLISFFYLIRHIYTHKNIKIKRGSIEFWLLAA
                     TPILIFAFYIPFRWNEEFFSFINPFKQFRGTGRFIFVFYYVYMVYVFVCMDRLYHKVQ
                     YPIFKIIFWFCCLITIYDIYNASNYLKYRFDSYGNKNAYNSYKDYTDKLTSKIKNKSD
                     YESIIVYPPSTEGTEKLWIDNDWNAKISSYWVSYFTGLPMVNVHSSRVSFDNCMQIFQ
                     LSGFYTMPKPIVQKFNPNKKQLVLLKTDRLNDDVPLVQNATFIDSINDIALLEIDLNS
                     ININKNKRVFWVDSSYTLLERKSYKKGNKLGLFINQTTNILALDLTNKKFNKLRILFW
                     YTPNYYKTSSVPVCSLYYIDAQNKEHFIKDWRESHTDTYNYKDGWLCVDYELNIENNF
                     KQLRFMIDGKQIYLSEFRVYGK"
     gene            complement(23062..24228)
                     /locus_tag="PLS16_00705"
     CDS             complement(23062..24228)
                     /locus_tag="PLS16_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59624.1"
                     /translation="MTVYIIKIPLEYKKISSVIYFISGISIGILFLLSLKIKEQPKVI
                     KFRYAIHFYYAILLLIFGYFLYQINFFFYSYPLNYKWADMLPIMKIMAERFIHNENPY
                     NPIYEIWDGMKPIYLPTMWILYSIPITLKIDIRWINIIVMLFGIFVFLYKRERKIQVL
                     DFLILALVAYFLIESLHHKNQDWYILTEEPLIVGYYLLLAYVLFRNKPILIGIAISLC
                     LLSRYILLFWFIGYLFYVYSDKLFLKKLLLTTIILCISLFIITQAYTQIDVFLNTPKG
                     YLNFMNSGLGTGVYDTTIKKSLGIAKFFEIKQLGFLHYLSMFFSIIVPIFLFYIYRSV
                     RFSKEVRNNYAIGILKISLVIFFNMLIIPMTYLFITSTLFSIALLSIVINKKTT"
     gene            complement(24379..25566)
                     /locus_tag="PLS16_00710"
     CDS             complement(24379..25566)
                     /locus_tag="PLS16_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNJ59625.1"
                     /translation="MNTSNSQISKTAYPILFAIAMAHGLNDTIQSVIPAMYPKLEQRY
                     DLTMAQIGIITLCFQLAASIFQPIVGAYTDKNPKPYSQVLGMFFSGMGIIVLAYAPSY
                     LWILVAVTLVGIGSSIFHPESSRVAFLASGGKRSLAQSIFQIGGNTGAAMAPLLVAWI
                     VIPNDQHYIIWFLLIVLTAKAILFYIGKWYSKILKAEQNGQKKTIVLPDLTQKQISIS
                     VIILLLLIFSKFFYMASITSYFQFYTMDKFGITEVQAQIYLFYFLFAVAIGTLLGGFF
                     GDKFGRKYVIWFSILGAAPLTLVLPYVGIEWTGVLIILIGLILSSAFPAILVYAQELL
                     PKKLGMVSGLFYGFAFGMGGLGSALLGFLADKTSVEYVYFICSFLPLIGLIAYFLPDL
                     RKK"
     gene            25743..26255
                     /locus_tag="PLS16_00715"
     CDS             25743..26255
                     /locus_tag="PLS16_00715"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNJ59626.1"
                     /translation="MLLIQKDMVVSVHYELQVEHDGNMTVADKSQDQPLVFIHGAGML
                     LPEFEQNLAGKTVGDSVSFTIKAENGYGVHDEKNIAHIPAESFHDENGKIDTEMVKVG
                     NVLPMMDNDGNQFQGIVVEVNDDNVVMDFNHPLAGKDLNFSVTVADVRPATAEEIEHG
                     HVHGPGGHHH"
     gene            complement(26339..27481)
                     /locus_tag="PLS16_00720"
     CDS             complement(26339..27481)
                     /locus_tag="PLS16_00720"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HNJ59627.1"
                     /translation="MKFATKVIHAGIEPDISSGAIMTPIFQTSTYVQQSVGVHKGFEY
                     ARTQNPTRNVLQNQLAALENGNFGISFASGLAATDAIIKLLKSGDHIISCNDLYGGTY
                     RIFNKIYGKLGIQSTFVDMNTIQNISDAITPNTKLIWVETPTNPMLNLVDIEAVVKIA
                     KQHNILVCVDNTFASPYLQTPLDLGADIVIHSATKYLGGHSDVIHGAIVVKDKAIADE
                     LYFIQNASGAVPGPQDCFLILRGIKTLHLRVQRACENAQKIADFLVAHPKVEKVNYPG
                     LSTHPSHELAKKQMRGFGAMVSFSLKADTLAAADKVLSSTKLFSLAESLGGVESLIGH
                     PATMTHASIPLEERKKTGVVDSLIRLSIGVEDADDLIADLEQALNA"
     gene            27623..29068
                     /locus_tag="PLS16_00725"
     CDS             27623..29068
                     /locus_tag="PLS16_00725"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HNJ59628.1"
                     /translation="MSIAKKLASETAAYSLSSILAKAINYLFGLFIVKYISTEEYGVY
                     SKLYAYAGFLLVFLTHGMETTFFRFLHKQGYKDKAYSVAFTSITNAVIFFIALVFIFI
                     IPIGNFVEESHRLIKIFAFIMVFDVLCALPFASLRAQNRPLKFAFYKILNIGIFIFFN
                     GLLFIGLPVLDIDFGISKVENIFIANLTASILTFILLAFQTEKFKFTYDAKLHKELWI
                     YGFPIMLVGIAGMTNEMLDRAMLTKLLPYDAATNNIQLAIYSFNYKFAMPISMFLQAY
                     RFAAEPIFFKEASQANDRNMYAEMMKFYIICACFIFLGIMLFIPAFQSFFIWYTPNAK
                     VLFEGVFMVPILLLANLCLGIYFNISTWYKITDKTHFGAIISGIGAVITIVLNLILVP
                     VWGYAGAAWATLFVYIIMVAIGFTLERKYFPIPYQYGRIGFYLLASLFLFVIYTYFIA
                     NNIESLLIKSAIALLILGIYVILVYKTEKRK"
     gene            29114..29557
                     /gene="dut"
                     /locus_tag="PLS16_00730"
     CDS             29114..29557
                     /gene="dut"
                     /locus_tag="PLS16_00730"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNJ59629.1"
                     /translation="MKKMILKIVNESGNPLPEYETVGSAGMDLRANLTEPIMLKPLQR
                     ALIPTGLFIELEQGYEAQIRPRSGLALKKGLTILNSPGTIDSDYRGEIKIIIINLSNE
                     TTVINTGERIAQMVIAKYEQATLKEVDELGETERGEGGFGHTGII"
     gene            29605..31410
                     /locus_tag="PLS16_00735"
     CDS             29605..31410
                     /locus_tag="PLS16_00735"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HNJ59630.1"
                     /translation="MHILVINCGSSSLKADIINTINKETVAELRVERISTTPEIKINQ
                     NEIAYTGIVNTEEIIRFSLIQLKQFDTNIQISAIGHRVVHGGDKYTQPILVNEDVELQ
                     IEQLISLAPLHNPSNLSGIRICKQVFEGLPQVAVFDTAFHQTIPNRAKHYAIDKTIAE
                     KYGIRRYGFHGTSLKYVSERVAEYLQTDIKHLRMVACHLGNGASICAVEFGRSAETSM
                     GMTPLEGLVMGTRSGDIDPGIILSLIHEHKMSVEEVEELLNKNSGLSGLSGIGNDMRD
                     ILKQAEEGNEDCRMAIQVYTHRLTKYIGAYVALMGGVDVLLFTGGIGENAATIRKRVC
                     NKLNFMGIVLDEDKNIYQKLTNEQDVIELQDSISRVKIVAVKTNEELAIALDTEKIVS
                     EKNKVNTIPKIPIAISARHIHLKQATIDLLFGKGYQLTEYKPLSQPGQYAANEKLTLV
                     GPKNKIENVRILGPARNYDQVEISRTDEFFLGIDAPIRESGNIAGSPGIILEGPNGTV
                     QIHEGVIQAWRHIHMHPDDAAMFGVHDKDIVSVDVEDKDRPLTFKNVLIRVSDKFKLE
                     MHIDTDEGNAAQIHSGEEGVLELGNVSCQLHEKNI"
     gene            complement(31407..32342)
                     /locus_tag="PLS16_00740"
     CDS             complement(31407..32342)
                     /locus_tag="PLS16_00740"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNJ59631.1"
                     /translation="MFEQRFDTVLDLFNGTDFPTWKEKIISDLKGKVFEDLITLTEDG
                     IEMLPIYTAETTKDYILNIPTRKTQDWQITEYITVENCEKANQEALNALQNGANTILF
                     NFKNNAFNQEQIAILVKDFLLDAITIYAKNIGNTSVHLLSEYSINYNPKIFVPQQKST
                     VNELLFAVHYLFQNEAEISNVHFNIGQNYFLEIAKIRAFRWLVAQINQLQIKNKQFVL
                     FSETGFENRNDDFIENNILRNTTEAMSAILGGCDALIIHPHENSNLGRRIARNIHHLL
                     YYESNFKQIPDVVKGTYYIEYLTYQLAKKTWEQLN"
     gene            complement(32399..33541)
                     /locus_tag="PLS16_00745"
     CDS             complement(32399..33541)
                     /locus_tag="PLS16_00745"
                     /EC_number="3.1.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF027980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ca2+-dependent phosphoinositide-specific
                     phospholipase C"
                     /protein_id="HNJ59632.1"
                     /translation="MRNFILFICISFCIFSCKKETNQRIKKSSEQINPQLDTLKFNQI
                     QCIGSHNSYRKRTDPDIYKLVSAIGGILPSDLNPKAWDYTHIPFNEQFDLGVRSLELD
                     IYYDPAGGRYYKRIGNALVGKSVDSRVPELMQPGMKMMHIPDVDYNTHYYTFKSGLKA
                     IKEWSNQNPSHLPIVLLVEYKEFGVGNVLPFFTKILPFTEQAVNDVDQEIIDVFGANS
                     SQIFRPDDLRKTYPNLKTAIQQEGWPKISTMRGKIIFVFYANNLYTKNHPNLEGRMMF
                     QFSPLGSDNGAFVKMDGSEEVSKIQAAVKAGAIIRTRSDSDTEEARTGNTKTREDAFN
                     SGAQIISTDYYLPDSRAGQKGWTDYKVIFPTQNFARVNVVNAPEII"
     gene            complement(33608..34540)
                     /locus_tag="PLS16_00750"
     CDS             complement(33608..34540)
                     /locus_tag="PLS16_00750"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNJ59633.1"
                     /translation="MKRILYFIATVICSSLLTLLFVRAASSPDGLFTFFNPNASQTTT
                     TSLPNQHVNAPKIPATLSFAGEKVPLDDIEIRERLDNELIVNSYKHSATILMIKRANR
                     FFPIIEKILAENNIPDDFKYLCMAESGLENVVSSAGASGYWQFMKSTAPIYGLVVNSE
                     VDERYDIEKSTIAACKYLTSAKASNGSWTNAAAGYNMGINGINNQANKQGDSDYYNLF
                     LNSETSRYIFRILALKVIYENQQKYGFYLDKDDLYEPYPIRETIAVDGYVDWVTFAQN
                     NNTSYKMIRRMNPWIRDPKLYNKERRTYQVKIAK"
     gene            complement(34547..35053)
                     /locus_tag="PLS16_00755"
     CDS             complement(34547..35053)
                     /locus_tag="PLS16_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HNJ59634.1"
                     /translation="MMEIKNKIAESGLITIDLEDYFPKEAIIAFDIKPLLFKELILKE
                     KDFRLALKAQNWVEFSDKTIAIFCSSDAILPQWSFMLITQYLLPFTNKIYYGNPKIVA
                     QNLLLDNINKINIESFINQKVVIKGCSQETLSSAAYVEISKKLIPHVTSLFFGEPCST
                     VPVYKKKK"
     gene            complement(35132..36043)
                     /locus_tag="PLS16_00760"
     CDS             complement(35132..36043)
                     /locus_tag="PLS16_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59635.1"
                     /translation="MAFYFVALLLSSPQNHTLKNTTLNFLYFLGITVFTAALGFIINE
                     WSDIADDKIAQKHNHLADSSSAYKVILLSGTLLGITLTLFVMTWNLKSILLYAIQISL
                     LLLYSLKPFRFKRYKILALILDALYSGTLFYILAICLYSNKLNYLLLASLSLWAFVRG
                     LRNFLLHLISDANFDKQLDFKTIATYYDKEKIMHFLKIYLLPLEIISFLTLLLQLPYN
                     FIFISSYIIFLIYIHQRQQYIIPFLVRRKSIISTNILREVNLYYELFFIYILIFVLAL
                     NQVYFLIFIPIALLLFPKSFNWFKHLF"
     gene            36301..38001
                     /locus_tag="PLS16_00765"
     CDS             36301..38001
                     /locus_tag="PLS16_00765"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNJ59636.1"
                     /translation="MVTDFNVNNDAVEQKEEVRVFEEYIERKPSSLFVRYFITQMHLS
                     GLLLGGGFAYLRHRKETKAKFSFGKDVFLRLFLWLHWIFLNKKLIKQPFKVQLRRRLE
                     MLGATYIKLGQIMSLREDVLPVEITDELKKLLDSLPAVKYERFAELVEENIQQPIHRV
                     FVDISTNPLGSASIAQTHKATLKTGEEVVIKLLKPGTRELIKTDSKIIKFMGRVLNLF
                     VPELQPKNMFTEFCDYTNKEVDFRLEAENAEEFAGNFTKHPDIVFPIIYKEYSNENMI
                     VMELFKGIKPDKKAYEKYNLQERERIVDIGAYAIIQMLYKDGFFHADLHPGNLMIMDG
                     PNGPMCGFIDLGMVGRFEETTRKAMLYYFNSLVMGDPENAARYLSALAKTDKYSDLDG
                     FRREFIGVGNKWLKNSNFKDFSLAKLIMESVVLGAKYRLYYPLELVLMVKAIITYEGV
                     GNVILPGFDIQKVSKKHIRTMVLGEVNPMDMLRQQLLNAPEIMDVIIKAPMLITESVK
                     RYESRLVEKKGKSGSESIKTMIFASCCILGASMMASAGLEWFTFVPLFIIGGIIALKS
                     "
     gene            38061..38507
                     /locus_tag="PLS16_00770"
     CDS             38061..38507
                     /locus_tag="PLS16_00770"
                     /inference="COORDINATES: protein motif:HMM:NF033205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HNJ59637.1"
                     /translation="MAKTIKHKIDGIDFNFHIWGINSPLVDYRLCWLLNNLLDWNFER
                     VDDIEISSEKDDSIIYFNAYKYENKEDFYTVEIIQNKNDGFVLLPELRNIDYLFIFDG
                     EEDYFDKDEITPIFQQIQGIQSIFEIEVDNLKSKYKLLMRHFNDTK"
     gene            38494..39924
                     /gene="pyk"
                     /locus_tag="PLS16_00775"
     CDS             38494..39924
                     /gene="pyk"
                     /locus_tag="PLS16_00775"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNJ59638.1"
                     /translation="MIQNNRKTKIVATVGPASNSLENLEKLYLAGVNVFRLNFSHGTQ
                     QQHLLVIQNIKKINQKHKSNIGILADLQGPKLRVDAIQNNAILLEEGRNIIVSTEEVL
                     GTNEMISVTYPHLSQDIKLGESILIDDGKIELKVTKIINKTQIECVIENGGILSSKKG
                     FNLPDTEISIPSLTEKDIADLDFIIQHHVDWVALSFVRKSEDIIDLRKRIAAQHGTQK
                     IIAKIEKPEAIKNIDSIIHDSDAIMIARGDLAVEVPMEEMPIIQKMIIEKCIDHARPV
                     IIATQVMESMIEAPRPTRAEITDIANGVFDGADAMMLSAETSVGKYPVKVIQAMDKII
                     LKTEEQNKIYFKNLSPKKDSPTFLSDAVCYNACSIAEDVEATALLGMTYSGYTAFMLA
                     SYRPKSKIFIFTSNPQLIYQLSLVWGVEVFFYDNFETTDKSIRDIIGVLKDAGKIQQD
                     DIVINTASMPIKGRGRTNMIKITIVD"
     gene            complement(39943..40482)
                     /locus_tag="PLS16_00780"
     CDS             complement(39943..40482)
                     /locus_tag="PLS16_00780"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNJ59639.1"
                     /translation="MLNSILFLAIGIISWTFLEYIIHRFLGHQKNGKHIIKKEHQRHH
                     AEVNYFVPIAKKLGLAIVVLFSSTLITGLIFNLKDGFLFSIGLASMYFIYETAHKLFH
                     IKEPIIRYGLRMRKHHFYHHFGNPKFNHGVTTAFWDRIFGTYHKVDMVKVPRKMPLIW
                     LQDQNKNLKTKYSNHFKIV"
     gene            complement(40572..42653)
                     /gene="ppk1"
                     /locus_tag="PLS16_00785"
     CDS             complement(40572..42653)
                     /gene="ppk1"
                     /locus_tag="PLS16_00785"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003917.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNJ59640.1"
                     /translation="MEKLTIINRDLSWLSFNERVLQEAENPAVPLLERLKFLGIYSNN
                     LDEFFRVRVATISRMTDFKKQAKNKMGFNPIKLLDEMQKQILVLQERYDNAYTNILAD
                     LEKENIIFVNEKELSKQQGNFVRQYFHDKVRLEVFPIMIRNDKSFPILQDASIYLAVQ
                     LYKKKAKTKHYSLIEIPTNSIPRFVEIPSEDDKKYIIFLDDVIRFNLKSIFKLYDFDI
                     IEAYTIKFTRDAELDIDNDVAVSFLESVELSLEKRKNADALRFIHDAKIPKDFLNFLI
                     KKMHLDKEAHVVAGARYHNFKDFIKFPTLNRNHLRYDYLPQIPHADLKNVHSILSKIK
                     EKDILLYYPYHSFDYFLDLLREAAIDPSVSHIRCTLYRIAKNSNVVKSLITAVRNGKD
                     VTVFIELRARFDEEANIEWANILTKEGAKVITGIPDLKVHSKLCLITRRERGQKIYYA
                     AIGTGNFHEKTANIYTDAMLLTSNQLLTKEVRNLFTFLDKNYYAPKFNRLLVAPFNLR
                     TKLEKLINREIKFAKMGLTASITIKINSLTDEDMIRRLYQASKNGVKVKLIVRGICSL
                     IPQLPKLSENIEVISIVDRFLEHTRVFIFHNNNEPEYFIGSADLMARNLDYRVEVITP
                     IIDKKIQQTLANIIDIQWQDNVKARIISGIQTNQFRYIDEKTSIRSQYKIYDYLLGNS
                     KKKIEKKALKK"
     gene            42788..43606
                     /gene="panB"
                     /locus_tag="PLS16_00790"
     CDS             42788..43606
                     /gene="panB"
                     /locus_tag="PLS16_00790"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNJ59641.1"
                     /translation="MSTAKSEIKRVTTHKLKEMKQRGEKISMLTAYDFSMAKILDDAG
                     IDILLVGDSASNVMAGHETTLPITLDQMIYHAQSVVRAAKRCFVVVDLPFGSYQGNSR
                     EALNAAIRVMKETGGHGVKLEGGQEIGESVIRILTAGIPVMGHLGLTPQSIYKFGTYT
                     VRAKEENEAERLIHDAKYLESIGCFAIVLEKIPADLGKQVAEALSIPVIGIGAGMHVD
                     GQVLVTHDMLGITAEFKPRFLRQYLHLYDDIKNATVQYIADIKAKDFPNEKEQY"
     gene            43728..44675
                     /gene="nusB"
                     /locus_tag="PLS16_00795"
     CDS             43728..44675
                     /gene="nusB"
                     /locus_tag="PLS16_00795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNJ59642.1"
                     /translation="MINRRSVRVKTMQALYAFQSSDTQSTNDFEQYLIKSIQSVKEQY
                     LFILLYIREIANYVEQDAHIKAKKYIQTEKDKQFSTKLLSNTFVQYLNHNEEFQNEIK
                     KSNTQLLINVDEVRKFYKKFSETAAYKSYLQNESEFNIDEDQKIIAFLFNNVLLEDEH
                     FLEIIEEFFINWNDDALLVVDAVREAIKKSKNELALHIGKQNVKSKIDELTKFGKDLF
                     FNVVHKKEEYIGLIEPKLKNWETDRLAMLDVLILRMALSEFIDFPSIPVKVTMNEYLD
                     IAREYSTSKSKDFINGVLDKIMQELKAKNQLNKTGRGLL"
     gene            44766..45722
                     /gene="metF"
                     /locus_tag="PLS16_00800"
     CDS             44766..45722
                     /gene="metF"
                     /locus_tag="PLS16_00800"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HNJ59643.1"
                     /translation="MKITEILEAEKGKNLISFEILPPVKGKSIEAITKILDPLMEFKP
                     SFIDVTYHREDYKYKQTAAGYFEKISIRKRPGTVGICAAIKYRYNIEAVPHFLCGGFT
                     KEETENALIDLAFLGINNILALRGDARGMEKKFIPEANGHAYAVDLIRQIEGMNSGIY
                     LDDDLVNPVHTDFCIGVAGYPEKHFEAANLEEDLQYLKDKIDAGGDYIVTQMFFNNEA
                     FFKFVEDCRKIGITVPIIPGLKAITRKSQLNSIPSAFYIDLPDALVKELRNAKDDIQA
                     KQIGIEWCIQQSKELLAYGVPCLHYYTMGDVKTTSAICSGVV"
     gene            complement(45700..47328)
                     /locus_tag="PLS16_00805"
     CDS             complement(45700..47328)
                     /locus_tag="PLS16_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HNJ59644.1"
                     /translation="MNQNWIYTTTIYQIYPRSFFDSNNDGIGDIQGIIKKLDYIKDLG
                     FETIWISPFYTSPQVDFGYDIADYTNIAPEYGTLEDAQQLIDECHKRKLKIVFDMVMN
                     HTSDQHPWFLESRKDKTNPKADWYIWRDKPNNWKSIVGPKAWKYAPERKQYFYSSFLN
                     FQPDLNYRNEEVKKTMFDICRFWLAKGVDGFRLDIFNCIIKDKDFRDNPFSLIRAIPS
                     HDYPGGNFQIRKYNVNQAGNFEFAKELRQVSDEFTPERMLLGEVFGLHSLKKQFLGKN
                     NGLHLIFLFDICFYKFNASFFRKKFLEYEREYPAPLKPTLVFSNHDQFRSIRRVGNNP
                     EKAKLVTLLQLTARAVPTSYYGEEIGMVNQKIPLKNAQDGMSKTFKWIPQIIVDALPV
                     PINRDNCRTPMQWDKTKNAGFSTADTTWLPVHEDFETCNVETIQQDKNSIYYTYKNLL
                     AIRKNSNSLQNGNLEIVDVNNDNILAYTRTKDTESLLILINFKHKKQTFSIPKNIEIT
                     EFIYGIKSKDFKQAELFGIDAVLLKCSIKQHQNK"
     gene            47517..50204
                     /locus_tag="PLS16_00810"
     CDS             47517..50204
                     /locus_tag="PLS16_00810"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HNJ59645.1"
                     /translation="MRKVFKGFFIFLFVLILSAFALPFFFKDKIVQLAYKEIEKRIDA
                     DVNIGDADLSILKNISNFPNIALALEDVTLVGKGAFAGDTLLNLGNLKTSLDLMSVIK
                     GDEYKIEAIELKDVYVDAIVNKAGMQNWDILKETPTDTSTASPFKLALKKFILDNVNL
                     EYNDFTNGDELKIENIEHQGKGDFSTEVFDYISKTSIEKLSLKQGLISYLKNSKLNFD
                     SKININQTENKYSFKDAKLTLNDLGLLFNGFVQTFDDKTNLDVQFKADKTSFKSILSM
                     IPAIYTKDFDKVKASGNLALDGSIKGFLQGENYPNFNLNLKVDNGKFQYPDLPTSVND
                     VFINANIAHAQGNLDNSIIDLSKLDFKMGTEPITARLKVLTPISDPNVDLMAKGKLDL
                     ANIMKIYPVEDIEQLSGNVNVDLAVKAKKSDIDKKNYTAITALGSIVSNNILFKSKEV
                     KDAVSISSLLLNFSPQFVALDNFKANLFESDFDMKGRLDNLIGYFLSKDAILSGNLNL
                     VSQQINANKFLPDSTSANQSKAQQAKDVVRLPKNIDFTTNTQIGKLLYDKLVIENLSG
                     NMNLKDEQINLNNMSANLLGGNAIISGFYNTKTDIPTAQINYNISNFDVQETYKFVNT
                     AQKALPFMKYINGKYNSNMNIKTNLYPDLSPNLNTLNGTASFKMPWANIEGATIINKI
                     AETTKIKQLQKLRLENIDIKTTFVDGRLLIEPFTVKSNKMDITIGGSQGLDETMDYAI
                     ALDVPWEELNQDATAFAKNLLAKNPIPQLNNAIPEVIRLNLNLTGTFKNPKITMGKPD
                     GKVGGGTMKDVVKEQAQQQIQNIKEDAIQVKEELKQQGKQTLDTLKTQVKDQAKQALQ
                     NLISGDNNDSTNTTKPIETIKKSGESVKDALKNKLPWMK"
     gene            50276..51907
                     /locus_tag="PLS16_00815"
     CDS             50276..51907
                     /locus_tag="PLS16_00815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNJ59646.1"
                     /translation="MRKPKNRILGGLLSILRNEIQSHRTLDGRRNFLKQTAKATVAFT
                     VASSIPAVFTSCKTATGNGASVNSNKILDIAILGGGMAGLNCANHLLDSGLSFRIFEA
                     DRRIGGRILTHYNDSLGLGIYPEFGAEFIDSDHEDMLALCKEFNLELLDMIAEQEKNN
                     WIKDIFYFDNRRISEAEIIKEFKKVAPKIAKDLDALGENYDTEEAVKFDNMPLSDYLK
                     SLKCAKWLKELFDASFVAEFGLDCKDQSTINFLDMVDTDTSEGFKVFGTSDERYRVKG
                     GNSEVIKHLSAKVGMDNIDINYEVNAIEELEDSTYLISFKNGEQVKSKSVVCTIPYTI
                     LRNIDLKLKGMSPEKRKCIDELGYGINTKLLLGYDSLPWRSKENNAMGYLFTTEMTNG
                     WDASVNKKEDNPNGAYIAFFGGDLSQELANKSFKNLYQPPTHVWRTGLPDETVDYYLS
                     RLDKIFKHSKEHYIGRYVFVNWIDYPFVKASYSCYRIGQWTSIAGLEMQPIGNFLFAG
                     EHCSAQFQGFMNGAAETGRRVAEEILKAKEVVTNN"
     gene            51978..52394
                     /locus_tag="PLS16_00820"
     CDS             51978..52394
                     /locus_tag="PLS16_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59647.1"
                     /translation="MFDELAKYKNKGHFFFEKGDVLKTKSKDVPNLPGVYYVIKLAKG
                     KVELVYIGKSGSIQQNGQFKEQLLNQRLNNKQDGMNREQYFLQKIELENIDALDIYWF
                     VTMDEQYQDLPSFVEGLLLQRYFEIYACLPKWNKSF"
     gene            52715..52951
                     /locus_tag="PLS16_00825"
     CDS             52715..52951
                     /locus_tag="PLS16_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59648.1"
                     /translation="MFCILESITATPINERQRLMLNKLFDGFDGKLQTSKWAKITKTS
                     TDTALRDIKDLIEKGILKPTDEGGRNANYKLYYE"
     gene            complement(52990..53622)
                     /locus_tag="PLS16_00830"
     CDS             complement(52990..53622)
                     /locus_tag="PLS16_00830"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ59649.1"
                     /translation="MKSELKKIVIIEDNPDVGSGFELLINATENYKVIQVFENCKDAL
                     VDIKTLKPDIVLMDIDLPGMNGIEGTSIIKSLLPKTEIIIITVFENSERVFGAMNAGA
                     SGYLTKNNGYKELIDALDEVVKGGAPMSAKIARMVVKSFAKETINNPLSKQESKVLSQ
                     LAEGKSYKSISNLLDVTLDTVKFHIKNIYIKLQVNNKEDAISEGKKNKYI"
     gene            complement(53633..54481)
                     /locus_tag="PLS16_00835"
     CDS             complement(53633..54481)
                     /locus_tag="PLS16_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59650.1"
                     /translation="MDTYGNPAFKNYIIENSSTHNIFTFGFVILLMSSLLISFNKVLQ
                     RTNQKLETLKQEQIEELQEKIKEKTEELNLLRKDLAKDFHDEMGNKLASITILSQSIG
                     LKMDIGKSEAEIKKLLNTIEERSIELYHGTKDFIWSIDFKSDYIDEWFIYTRDFGEDF
                     FEKLGISFYSSSDIKQDSNLTFDTNVSRQLIYILKEIMTNTAKHSNATQTNLQLLLLQ
                     ETNEIDIVFSDNGNGFNIENIQQRGINNIRERIAKINGKLDINSDKTTGSVFKIRLPL
                     QHNYKL"
BASE COUNT        19205 a         7541 c         8870 g        18976 t
ORIGIN      
        1 tccatttaac atttgagcat attctcctat agtcattccg taaacaattg gaattttttg
       61 cattccaata aaactttttt gagtagtttc caagatagga ccatctacat aatgtccatt
      121 tggattgggt ctatctaaaa cgatgagaga tttattattt tcagcacaag cttccattac
      181 atattctaaa gtagaaatat aagtatagaa acgaacgccc acatcttgaa tatcgaacaa
      241 taaaatatct acaccaacta attgttctgg tttgggtttt ttattattgc catataatga
      301 aataattggt gtttttgttt tagcatcaat accacttttt acactctcgc cagcatcagc
      361 tgttcctcgg aatccatgtt caggtgcaaa aactttgact acatttattt ttaatgcaat
      421 taaagtatct aaaaggtgtg tattgccaac aactgaagtt tgattaacga ccaatcctac
      481 ttttttattt tgtaataatg gcaaatattc ctttgtcctt tctgctgcca caattacttc
      541 tttgccaaaa gaaaaattag aataaaggca aaataatatt agtccaagat atgtgatacg
      601 cttcataaaa atcaaatgta caaatatcat gcaaatttct attctaaatg aatagaacaa
      661 atttttatta ctattctaaa gattactcta ttattgcagt agcctcaatc tcaattaata
      721 aatcaggatt aattagtttg gggatttcgt acattgctgt tgttggtttt atttcagcaa
      781 atatttctcc atgagctttt cctatttctt cccaacgaga aatatctgta cagaatattc
      841 ttgtacgcac tacatctttg tagctagcac ctaattcgct tagcactttt tcaaatttgt
      901 taagaatggt ttttgtttgt aaatacatat ctcctttacc aaccacttct ccatttacag
      961 ttgcagttgt tcctgcaatt tcaattgtat ttccaatttt tacagctcta caatagccaa
     1021 tttttgtttc ccattcgcta ccttctaaat aatttactct agtactcatt atattttttt
     1081 agagccaaat atatctaaaa ataatcaaga aactttaaag cttgaattat ctacaaatta
     1141 aatttttttg tacaccaatt aaaatttata accaagcgtg agtgcaatta ccgagcgttt
     1201 gaaattattt tcagaagtat attgaaattg tggagcttca ttttgtaaaa cataaggggt
     1261 gtaactttct ttgccttttg taaattgata agctaaatca attgcaaatt tgttttttcg
     1321 ataaccaaca cctgcactat aaatgaaatt caattgtcca gattggtctg taacaaagtt
     1381 tttgtcgaat ggactacttt ggatttgtat cccacctctt aatctaattg gatctatttt
     1441 tgcttctaat cctaatttta atgtatggta taatttgtac ttgtttttaa tcgtttggtt
     1501 taatgctctt tgttcttctt ttatatcagc atcagcgttt ttgtaattat attttgaagc
     1561 tccagcatca ctcaattcgt attcagcagc gattaaacca atcttagaaa tataactcat
     1621 tccagtattt aacttccaag gtaaacgaag attgtatttc attaaacctt caggcgattc
     1681 agcttctgcg gtgtaggtaa cataatcagc atttattttt gagttaaatg catcatttaa
     1741 aaacaataca cttggtgttt gaaaggcgaa agacaatcgg acgtttttag ataattcagc
     1801 caataaacct aatttcagat ttacacctaa gccactcact ctaaagcttg aattttgttt
     1861 aaaaaaatta aaattatagc ttgaatctgg agtgtctgtc tctttatgga tagtactttc
     1921 agtaaaattt aaagttggaa caccgattgt tgcaccgata tgtatttttc ttttttcgcc
     1981 tgaataggta cttgctactg caattgccat ttcatttaga ttgccttttc tttgaataac
     2041 gatatcttgt tgtacattcc cattattaat agctgaataa attcggtggt cagtagtatc
     2101 aaaataaact aaatctgtat tgtatgcgag gcttgcatta aaaggaaaca tattttccaa
     2161 gtcatttgtt gtggacgcat gttcatctaa aagctcaaaa tatttatcaa ctaaactatt
     2221 gtgttgattg aatccagaaa tataaaagtt ttgatttagg ttagctaaat tattaaaact
     2281 gaaacccact tttggagtac tccatttgcc ataatttgat acatatttgc ttaccatagc
     2341 tgcactaaaa ttgtaaaccc caaattttag tttggaatct tctgtaatct ttccaatata
     2401 atttgaatta gaattaaaaa ttgaaacaac tggcgtaagt gatacttcag aggaagtaat
     2461 cctacctaaa gcagctggat tggtatgtat agagcttaat tctccaccga tactacccat
     2521 tgtgtttcct aagcccatat ttctagcact tccgttactg tattgcatac ctaaacgcaa
     2581 tgcatctaca tctgattgtg caattagatt ggttgtatta attagtaatc ccaaagtgaa
     2641 tatggaaaca agttgcttaa tcataggttt tgttttttta gaattattta ggtgttttag
     2701 ttactttaat accagaaggt gtagttttat tagatggcga tggtgtttta tagttattgt
     2761 tattattatt cattgatggc gattgtttgt agttgtcgcc accactgtta ttatttttat
     2821 attgaggttt gtaattgtta ttatcgcctt tctcaatagg ttggtattgt ggtttagaat
     2881 aattagaatt tgttttttta ttgatttgat agttctcgtt gttattattt ttattgttgt
     2941 tttcaggata agaattgtta ttatagctcg gtttattggt tgagatgttg ggtcttggtg
     3001 tgtaggtagg tgcaggtttt gatggtttgt ttacagatgg ataatagtta ttattccaat
     3061 agttgccata gtaattattg ccataataat tgtcatagcc ataaggaccc caactgttcc
     3121 atgagttgta tctatttccc caactccaag gattccaact attccaagaa ttccaatgat
     3181 tgaaaccata ccacggattg ctccatgagt tccatctgtt ccaaggatta taattattca
     3241 taatgataat ctgagtattt ccgccatacc aatttgggta gaaagaagaa cctgagttcc
     3301 acatccaatc attataatag gagttgtatc cattatagcc ataataagat ggacgacatg
     3361 cattaaagtt tacactaaaa cgcacactcg gattgtgaaa tcttctaatt ctatccgcat
     3421 atcttgaatt gccattatta ttataagaat catacccatc ttcttcgtaa taattgctat
     3481 tttcattatt atagctattg ctagaatagt catcattatt atagttatca tattgataac
     3541 tattgttgtt ataatactga ctaccagaac ttgaaggcga atatgttttt tctgtcttta
     3601 tttttttctc tgttggtgta aagtcatcat acacatcatc tgcataagtt gaatttattg
     3661 ccaagggcaa aaccaaaaac atatttaaaa tgaatctttt cattgtttta taatttaatt
     3721 atttagcaaa ataaattatc ctacatttgt aaaaccaaaa ttacaacaaa taagtttcaa
     3781 aaagtgtacc aatatttgtt aaaaggtatg ataaatagtt agattaatct aaaaatatac
     3841 attaataaaa tgagtaaagg aattacgtca agagcagaaa attacagtga gtggtataat
     3901 gatttagtat tgaaaggtgg tttggcagat tatagtgcag ttcgtggttg tatggtcatt
     3961 aaaccctatg gatttacact ttgggaaaat atgcgtgata ttttggataa gaagttcaaa
     4021 gaaactggac atgtgaatgc ttattttcca ctatttgtgc caaaaagtct gtttgaagca
     4081 gaagaaacaa atgctgaagg ttttgcaaaa gaatgtgcag ttgtaacaca ttatagatta
     4141 aaaacagatc ctgaaaataa aggtaaatta atcattgatc cagaagcaaa gttagaagaa
     4201 gaattagtag tgcgaccaac ttcagaagct attatttgga atacttataa aaaatggatt
     4261 caatcataca gagatttacc aattttagta aatcaatggg caaatgtagt tcgttgggaa
     4321 atgcgtacac gtttgttttt acgaactgcc gaatttttat ggcaagaagg acatactgca
     4381 catgctacaa aatcagaagc agttgaagaa actgtacaaa tgattaatgt gtatgctgat
     4441 tttgtagaaa actatatggc aattccagtt attaaaggtg taaagtcggc taatgagcgt
     4501 tttgcaggtg ctgaagatac ttatacaata gaagctttaa tgcaagatgg aaaagctttg
     4561 caatcaggaa catcacattt tttaggacag aattttgcaa aagcatttga tgtgaaattt
     4621 gtcaataaag aaaataatga ggaatatgtt tgggcaacga gttggggcgt ttcaacccgt
     4681 ttgattggag cattaattat ggcacattct gatgatgaag gtttgattat tcctccaaaa
     4741 attgcacctc atcaagtggt tattattcct tttttatcta agaatgttga agaaacacaa
     4801 tcaatgattg ataaagccaa tgaattaaaa caaaaccttt caaaattagg tattcgttca
     4861 attgtagata gtgatgaaac caaaagacct ggatttaaat ttgcagaata tgaattaaaa
     4921 ggtattcctt tgagagtagt gattggccca agagatttag ccaatggtgt agtagaaatt
     4981 gcaagacgtg ataccaaaga aaaatcaact tttcctatag aaagcttgca agaaacgata
     5041 gttggtttat tagatgaaat tcaaaataat ttatttgcta aggcaaaaag gtttagagat
     5101 agtcatatta ctgaagtaga tgattttgaa acttttcaaa aagtattaga agaaaaaggc
     5161 ggctttattt ctgcacattg ggacggcaca cctgaaactg aagataaaat taaagaattg
     5221 actaaagcta cgattcgttg cattccatta ggtaataaaa aggaagaagg aaaatgtgtg
     5281 ttcacaggca aatcaagtca agaaagggta ttatttgcaa gagcatatta attattccat
     5341 ttaataaaaa aaaacacgct taattaagcc ttttaattat tgtcaaaagc gataagctca
     5401 ctaaaattgg gtagtttaag tgttgtttta gtgattactt tacatagtat tgattattag
     5461 tgattttttc ttaaaagatt tctaaaactc tgttaaacta catatactat ttttgtatta
     5521 tcaaaatata caataatgaa aacagaccaa atcgacaaat tcagagaagc tttagatgct
     5581 ttagtaaatg attttaatat taaagttgaa gagtttagaa ttaatcttca aaatttttca
     5641 tctgatgttc aagttcaagt tcaaggacaa agagagaact tacatgagta tggtgaaaaa
     5701 ttaaaatcaa gaattacgaa tatcgttgat atcaataaaa ttgataaagt aagagaggat
     5761 ttgttagcag aggctgaaaa tgtaatggat gatgcaaaac aaagattaga tgatttcttt
     5821 aaagtaatta cggataaagt aaatgcagga gagaaaaaag ctaaagaagc agctaataat
     5881 gttgcaaaac aagctaaggc agctacagag tctgtagaga aagaaattaa aaaagctgca
     5941 aaagatgtag aatctaaagt aaaaaaagct gaaaaagaaa tcgaaaaaac tgcaaaagaa
     6001 gttgaagcga aagtaaagaa agcggaaaaa aatattgaaa aagaagtaaa acaaacagtt
     6061 gctaaagctg aaaaagaagt gaaagcaaat gtttctaaag ttgctaaaaa agtagaaaaa
     6121 gtagcagaag aagttcaaaa aaatgtagca actaaaaaag cacctgcaaa aaaagcagca
     6181 gctcctgtag ctaaagctcc agcaaaaaaa gttgcaccaa caaaggcaac accagcaaaa
     6241 gctccagcta aaaaggcaac ggctccagct gcaaaagcac ctgcaaaaaa agcagcatct
     6301 aagaaatagt ttttttgttt agtgggatat ttattttaag aacctgtatg attgcaggtt
     6361 cttttttatt tcattaatac ataacaacag ctcttaaaat ttttaaatca taataattaa
     6421 cttagcagtc aaaattcctt ggaatgaaaa tgaatataaa acaactatta gaacaaaaaa
     6481 gtattggcac gcaagcaaca atatctgcat gggtaagaac aaaaagacaa agtaaaaatg
     6541 ttgcatttat aaatttgaat gatggttcaa caattcataa tctacaagta gttgcagaga
     6601 caattgaaga agctatttta gctaaaataa atacaggtgc ttgtatcaaa gtggaaggaa
     6661 atattgtaga aagttcagga caacaagcca tcgaattgaa tgcagaaaag attacgatat
     6721 taggcgaagc tgacccagaa aaataccctt tgcaacctaa aaaacattcc ttagaatttt
     6781 tgagagaaat cgctcatttg cgtccaagaa ctcaaacttt tagtgcaatt tttagaatta
     6841 gacatgcttt agcatttgca attcatcaat attttaatga taatggattt ttctatttgc
     6901 acacaccaat tattacagcg tctgatgcgg aaggtgctgg agaaatgttt agagttacaa
     6961 ctttagattt gaacaaattg ccaaaggacg aaaataataa tatcaattat aaagaagatt
     7021 tttttggaaa ggaaacaaat cttactgtgt caggacaatt agaaggcgaa ttgggtgcaa
     7081 tggcattgag taaaatatat acttttggtc caacatttag agctgaaaat tctaatacat
     7141 ctagacatct tgcggaattt tggatgatag aaccagaggt tgctttttat gaattggaag
     7201 ataatatggc attagctgaa gatttcttaa aatatgtaat tgcttatgct ttaaaaaatt
     7261 gcaaagacga tttagaattt ttagagaaaa gattattaga agaagaaaaa tctaaacctc
     7321 aaaatgagcg aagtgaatta gctttattag ataagttgaa ttttgtagtt tctaatgatt
     7381 ttgaaaaatt gacttatacc aatgctgttg aaatattgaa gaatgctaaa aagaaattcc
     7441 aatatccagt cgattgggga accgatttgc aaagtgaaca tgaaagatat ttggtagaaa
     7501 agcatttcca aaaacctgta atacttacag attatccaac ggaaataaaa gctttttata
     7561 tgaagcaaag tgaagatggt aaaacagtaa gagctatgga tgttttattt ccaggaattg
     7621 gcgaaataat tggtggttca caaagagaag ataatttcga aaaattgaaa aacagagcgg
     7681 ctcaagtggg tattcatgca gagcaaattt ggtggtattt ggaaactaga caatttggag
     7741 cttgtccaca cgctggattt ggtttaggat ttgaacgttt agtattattt gttacaggaa
     7801 tgacgaatat tagagatgtt attcctttcc caagaacgcc aaaaagtgca gagttttaat
     7861 ttatagtttt ggagaaaatt agccataaaa aaatcatttt tgatttaggt gccgtcatca
     7921 ttgatttaaa atcggaatca gattggtgga aggaagattt atttccaaat ttcaatcaag
     7981 agcaattaga aaagctattt aaagaaggct tttttcatga ttttgaaact ggaaaaatta
     8041 gtacaacaga ttttgtaaaa gtgatggaga gtattaaaat aaatcctgat attgcagttg
     8101 aaaaagcttg gaatggtatt ttaaaaacga ttccaaaaca taggatagat ttattgaatt
     8161 gtctgaaaga aaataatcag gtttatttat tgagtaatac aaataccata catacaaaga
     8221 ctattcatga atcattagag tttgactttg gtatgaatat ttttgatact atttttgata
     8281 ttcaattcta ttcgcaattg gtaggaatga gaaagccaaa tactgaaatt tatgagcatg
     8341 ttcaacaaac tattggagat tccaattctg ctaattttat ttttttagat gataagcctg
     8401 agaatttaat agcacccaaa aaattaggtt ggcaaacaat tttggtggat agagatattt
     8461 ccgaattaat cgcttaaatt tattctatac aaagagcaag tttatcgttc aattaattaa
     8521 tattatgaaa ttttcagatt atagaataaa tgaagacatc aaaaggaatt tggaacaatt
     8581 aggtttcaaa aagccgactg atatacaata taaagcgatt ccatctattt taaaaggaga
     8641 agatgtattg gcaattgcac aaacaggtac tggaaaaacg gcagcttttg ctattcctat
     8701 tatccatttg atttacgagc aaaagaaatc aagtaggagt gatgctatca aatgtattat
     8761 tatggttcca acacacgagt tggcaatcca gataactgaa gtttttgaaa aaattgctta
     8821 taaaacgaga gtaaaaacct tatgtattta cggcggtgtc gatcaagccc cacaaatcga
     8881 ggctctgtat aaaggcgtgg atattgtcat tgcgacacca ggtagagtgt ttgatttggt
     8941 gcatcaagga gcattagcct taaataaaat caattatttg attttggacg aagctgacca
     9001 catgttggat ttaggattta tcaaagatat tcaagatatg attcggtttt tgccagccaa
     9061 aaggcaaacc cttttctttt ctgcaaccat caatgaaacg attaagaaat tagcctattc
     9121 gttagttcac aatccaattc gtatccaaat ttctcctaaa gatccagttt ctaaaaatgt
     9181 acaacatgct gtagctttta tagagatgga tgccaaacgc tattttttag aaagagtaat
     9241 ttcagaacat aaggaaagta aaatacttgt ttttgtaaga acaaaagtaa gagccgagcg
     9301 tgttgcaaaa gcaatggagc gtgtaggtat tcaaactttg acgatgcaca gtgataaatc
     9361 tcaagaagaa agattttcta ctttaaagga atttaaagat ggaaaagtga aggtcttaat
     9421 tgcaacagat attaatgcaa gaggtattga tataccaaat gtggactatg ttgtgaatta
     9481 tgatttgcca gaaaaatcag aaaattatgt acatcgtgta ggtagaacag gacgtggtgt
     9541 acaaaaagga caagctatct cgttttgtag ctcagaagaa aaagaaattt tgaaagaaat
     9601 tgagactttt ttgcacaatc caattcatgt gatagatatg gaacaagcag aacaggaaga
     9661 aatcctattt ttggcaaaag aaaaggaaat tcctgaaggc gattggcgtt ctttgatggc
     9721 aaaagcggaa gaagaggagc aattgtataa agaaaagaaa aagaagaagc gaaaatagta
     9781 attggataag aaataaagcg taattttgta gccatgcaat atcaagtaga aataaacgta
     9841 atgccattaa aggaattatt agatcctcaa ggaaaagctg tagaacaaag tttaaataat
     9901 ttgaatattc acgctttaaa cgttagagta ggcaaaagaa ttgaattaaa aattgatgct
     9961 acttcaaaag aggaagcttt tgaattaaca gagcaagcgt gtaaaaaatt attgtataat
    10021 ccagtaatgg agttttacaa aattacagtt tcggaactct aatacgaaaa gttagcttat
    10081 cttaattcca ccaaggtaac gccgtttccg ccaaattctt ctttttcaaa atagtaattt
    10141 ttgatataag gataattttt taggtattga tggatataag tccgcaactt tcctgtacct
    10201 ctgccatgaa ttatttttaa atgatgaatg ccgtacataa tagattgatc cataaatgtt
    10261 tctaatgcat tgtgggcttc ttcaaccaat ttgccacgca aatcaatttc gctttcaaac
    10321 ttactttttt ctataatttg cttggaagat ttttgtaatc taaattcttt tttggtattt
    10381 gctttttctt ccactaatac caattgatgc aatttaaaag tagttcgcat actaccgaaa
    10441 gcaacgatag cctttttgtt atctatcgaa atgacttcgc caatttcagt gccttcttct
    10501 aagcgaactt ttgaaccaac ttgaattata ccatctttct tctgttggaa attagagatt
    10561 tctttttgtt gtttttgttc tatcgtttta cgttgttcat caatattttt tctaagttca
    10621 gtataatctt ctttcttttg ttggctttct ttccattctc gcatttgctt ttccaacaag
    10681 cgactttgtt cattataaaa atccaataac ttttgctcat gagattttgt taatcgcttt
    10741 ttgtttttct caaattcttc ttttaattgc tcataatctt taacaatagt attgagttgt
    10801 gtttcctttt gttgcacatt ttttcgtaaa cctttgatat aatgtttttc aatttgtact
    10861 tcgtttagtg tttcatctaa agctttttgg ttatgtgctg ttttttgttt tgctaactga
    10921 atgacgtttt gtggcatacc aattttttga gcaatctcga aagtaaaaga acttcctggc
    10981 tgaccaattt gtaattcata tttaggttgt aatgctactt tgtcaaatgc cattgccgca
    11041 ctttgcattc cttttgtttg tgctgcataa acttttaaat tattaaaatg cgtagtaaca
    11101 caaccaaaaa catttttatg atgtaagatt tctaaaacgg cctcagccat tgctccgccc
    11161 aaacttggat cggtgccaac acccatttca tctaataaaa tcagggtatc tttattagat
    11221 ttctgtacaa aatatttcat tttttgcaaa tgagcggaat aggtactcac atcattttca
    11281 aaagattgct catcgccaat atccgaaaaa atttgattga aaacacaagc gatagagcct
    11341 tcatcaacag gcaataaaag tccaaattgc agcatggttt gcatcaaccc taatgatttc
    11401 aaaacaacag attttccacc agcgtttggt ccgctaatta ctaaaattct attttgctca
    11461 tcaagatgta aagtattggg aataatttga tgcttatgtt ttctaagatg atattctaaa
    11521 gctaaatttc taaaattttt tagataaatt actttctcct ttctaatttc tggtaaagta
    11581 cattgatatt ctattgcgag atttgctttt gcttgttgtg catcaaaata agctaaaaat
    11641 tcttgtgtcg tttgaatggc atctaattca ggacgaattt tattagataa attaataaga
    11701 attttttcaa tttctttctg ctcttctaat atacaagaaa tcaattcatt gtttaaatgg
    11761 acagtttctt caggctcaat aaatgtaata cttccattag aagattcatc aacaaaaata
    11821 ccttttacac tccgcttttt ttcgcttaaa actgctaata cttttctttc atttctaata
    11881 ctttcttcag tatcgtgcaa ataatctttt gctttgtagt gtgtcaaaac acgtttgaaa
    11941 ttctgttgta tttgttgttg tatttgcgat ttttgttttc tgatttgttg caaaatttta
    12001 gaaacacttt cattgataat ttcttgttca ataataattg tcttttctat ttcaagaagt
    12061 acagcatcta attctggcac ttgattaata atctctgtta atgcggtata tctttctgaa
    12121 tcaatttttt tagctgtatt ttttaattgc tttacggttt ttagtgcatt gtataattga
    12181 ataaattcgg aaacttggat aatgtaattt tcaatatcaa ttttatttaa aaaagacatg
    12241 gaattaaagc cttgatgtgg aatttgtaaa ccttcttcaa tccaagattt tatttgatgt
    12301 acaattttta attctgtttc aagtttagaa tgctcagtta taaaaccagt attttgaata
    12361 tgatttttac ccaaagaacc aatgcattta ttggataata aatctataac agtattgaaa
    12421 ttaatcttct cgggtgtatt ggtaggaaaa aaatgcatag gaatgcaaaa ttacgatttt
    12481 ttatttcact tcataggcga aactggagct tttagtgtat tccaagtgct atccgtcaaa
    12541 gcattaataa aagaaatgat gtctttcgtt tcttttgaag ttaggtttag tgattgatcg
    12601 gaaagggttt gattgggaat agaaataaaa gcagcaccac caccacgatt gtagaaatct
    12661 aaaacatctt caagcgtttt gaaacttccg ttgtgcatat aaggtgcagt atattcagca
    12721 tttcttatcg ttgatgtttt gaaggaattt ttaaaaaaat caaactttaa taatttgtat
    12781 cgaccgacat ctgaattttg aatgtagttt aaagtatccc aatttacggg aacgcccaaa
    12841 acttctgatt ctgattcaga ataaaatggc ggtactgttc caaaaaaagt aggtgcaaaa
    12901 tgacaagtac cacatttggc tttgcccata aataaattgt aaccattttc aatttcttta
    12961 tcaattattg gcgattcatt tcgcatatat ttatcaaact tagagtcaag tgcagtcagt
    13021 tggtgttcaa aagctgcaat tgctgcgttg atggttgctg cgttgatata attttcagat
    13081 gaaggatata attgtttaaa tgtttcgttg taatatttgt cgttttttaa gcgttcaaca
    13141 atggtatcat aattgttgtt aaattcttga tgattttcta ccacttgact ggcttgatgc
    13201 tctaagtttt tagcccttaa atcataaaat aaattgcgtt gataaacact gtttaaaatg
    13261 gttggtgtat ttctatcctg aaaaattcca acttgatttg tcaatgaagt tgctaatcca
    13321 tcagtaaaga aaagttctgg ttgatgacaa gtagcacaac tcatctttcc atctttggat
    13381 aatcttttat caaaaaataa atgcttgcct aattcaattt cagtttggtt aggaattttg
    13441 tatttattga cactataaaa aggcgtataa agaaaatcct tattgtaaat attgtcagag
    13501 tataaattta cagctcgaat gatattgtaa attgcttcat tgagtggaat ttcactgtct
    13561 tttaaaattt gagaaaataa ggcatttaat ggatgaagat attgctttgt aaaatataag
    13621 cgttctaagg tttcaaaatc cttttgttct aaatagttat tcgctgattt aattaccaaa
    13681 cgtgtttgat taattatttc ttgaacattt gatttggttt gcggtgctaa taatgctaaa
    13741 caagtatcaa tagatagaaa atttgcttga atatcttgaa tagaattacg agtggtttcg
    13801 caatcaaaat cagtaatatt taaggtttct atttttataa tattgtattt taaggcttct
    13861 aaaatggttg attcagagat aagttctgat tgtactattt cataaaattt caaattccta
    13921 tattgaaact ctttgattaa atttttaaaa gcttgttttt ctattttatc ttcagccagc
    13981 atcgcttcaa tgtcaatcaa tacgtgaaaa gcaaaagctt ctataataaa attacgtttc
    14041 tcatctaaaa caagagggat tttaaaaata tcttctgcat tataatccgc tataaattca
    14101 atttttttaa aatgaaaccg agcctgttta aataatggaa taattgttga tttgtcatct
    14161 ataatactat cttgtgcatt aatttcagtt ataatcttat caaggcttac ttgcaaatct
    14221 ttagaagacg cacttaaata actttttgtt tgttgaatga aatgagtatg ataagtttca
    14281 taattcaatg aaacaatagc cagtgtaatt attataagta ataatttaat tttcctcatt
    14341 caatctatga aaatacaaat tttaaagtaa atataggata ttacttatta gagaatatag
    14401 aagttatatt gattatatac tattctattt aaaactatta atgcaatatt ttaatgttta
    14461 tgtacaaata cagattttgc ataagcgaaa tttgtgccat cagtttgatt ttggggaact
    14521 ccgtttttcc atagttttgc tacatattta cttccattat cttcgtaacc tactacatag
    14581 acatcatttt ctagcacata agtagaaaat gcgtaagcat tatgagtgcc atctgtgagg
    14641 ttttgagcaa taccgttctt ccatagtttt gctacatatc tacttccatc accttcgtaa
    14701 ccagctacat aaatatcact gccttgtaca aaaacagaac gtgaataagc atcttttgtg
    14761 caatctgaga ggttttgagt aaaaccattc ttccaaagtt tagctatatc tttacttcca
    14821 ttagattcgt agccaaccac ataaacatca tttccttgta caaaaacgga ttttggatat
    14881 gcaaaatgtg taccatcagt tagattttga gcaactccat ttttccatag cttagctata
    14941 aatttacttc cgttccattc atatcctgct acatagacat cattaccttg cacaaaaacg
    15001 gattgtggat atgcatcatt tgtgccatca gtgaggtttt gaacaactcc atttttccat
    15061 agttttgcaa ctgtattact tccattattt tcatatcctg caacatagac atctattatt
    15121 tcaggctttg tacttttgtc ttttttacaa gcataaaaaa agggtactta cactacaaaa
    15181 tataaatgtg atgaaaatga tttttttaat atttttcata aatagtgaat attattattt
    15241 aaagttaagt ataaaattga tttaagtcaa caaataagtc aatgtttgtg tcaagaatca
    15301 tacgagattg agtataaaga taaattactc tttatcctat atttaaaaaa atagttttat
    15361 tagatgctat aataattatt gtgcctaaaa taattaataa ttgtagagtg ttgctaagac
    15421 tgtttcacca actgtttgaa gcgtattttt atctatattg tcgatagtgt catcgtgtgt
    15481 atgccaatgt ttagcaaatc cagattccgt ttctaaagtt ctataaataa tatctatagt
    15541 tggtgttgtg gttaatgtat tgatataata atggtcatca ataattggct gagtaactct
    15601 ttttataaaa tattttccat gccctaaact tgcagcattg ttccaaactt gtttcataaa
    15661 ttcaggcgca tactgcatag atacgccttc aaaagtaaat gttgcatttc ttgcacctac
    15721 catatctaat aatattccgt tgcttgcact ataatttgca atatgtggat ttttgcacca
    15781 atattgagtt cctaaagcat agaaatctct gccactatat ttagcttctt ctgaagctgg
    15841 tggtccataa tcttcaacat caaaaaatat aatatctatt cctatttcgc ttggtttgat
    15901 ttgtaattgt ctagcaattt ctaaaagtac agcaacgcca ctagcaccat catcaacacc
    15961 taaatttgga gtgctttggt ttttatctgc tctatctgaa aatggtcggg tatcccaatg
    16021 ggcacaaagt aataatcttc tttttatagt tggattaaag cttccaatga tattataaca
    16081 aggcactatt tgtccattat agataggtaa ttttgtggtt tgaacaatta cctcagctcc
    16141 atattgtttt agtttattag acaagtaatc ggcacatttt ttttgtgctg gtgtattagg
    16201 gattcttgca ccgaaatcaa tttgttgttc tataaatgaa taagcagaat cagaggagaa
    16261 acttggtttt tctaatttag atgtagcaac taatacctca gcttgttcag ttgtgttcgt
    16321 tttgcaagca acaaaagtaa aacatattat tagtaaggtt aatagacaaa agcgattcct
    16381 aagcttcata ttttgaactt ttatgcaagg tacagaaaaa ctaaatttta tcaaagatag
    16441 gtatatggat ttctgttttt cgttttctat ttaaacatta tttggtattt ttatacattg
    16501 aattgacttg aatgagcgaa ttaaaagata ataaatggaa acaatggtgg cagaaactga
    16561 aaaatgtcta tcgctttcaa gtcgttgagg agaaaactta tgatgtaaaa tttgtacttg
    16621 aacttaatag attgaatgtt attacagttt caggaatttt aattgcaatt cttacactac
    16681 ttaatttctt ttttatagct tatacacctt tgaagcaata tattcctggt tatggaacag
    16741 tggatagtcg aaaagaaatt gttgatttga aactaaaaac tgaagaggtg gaagataaaa
    16801 ttgatgctaa tgaaaagtac aataagaatc taaaaaatat tttaacagat aagatagaag
    16861 taaaatcgct agaggataat attacacctg ttgttgttga tcctgatgtg ttggttcaga
    16921 tttcacctga tgaacataaa ttaataaaag atattgaaaa aggattgaca aatgcggagt
    16981 tgtacgataa tttacaatac aaaaagacga gtaatacatt agctgcttta aaagctgttc
    17041 ctcctataaa tgcttcgata gtttcaaaat ttaagaatag taaaaatgct tttacaacga
    17101 tttacaaaac acccaatgct tcagcaatta ttgcgacgat agatggttat attgtatcaa
    17161 tcgatgaaca gaagaatgga gattttactg taattattca gcatcaaggt gatttagttt
    17221 ctaagtatag aaatattaaa caagtaagca aaaaaagtac taattttgta gaaagtggcg
    17281 aggaaatcgg aactgctgat actcaaaata atttagagta cgagttgtgg tacaaaggaa
    17341 ttcctataaa tgtagaaaaa tattataaaa agtaaattat gtttaattcc aaaaattcta
    17401 ataataatgc ttctagttta gtagctaatc atatcaatga caatacaaat atcaatggag
    17461 atattcaagc aaatagtgat atgcgaattg atggtaatgt aaaaggaaat atagtttgca
    17521 catctaaagt ggttattggt agaacttcaa gagtagaagg caatatcaag tgttcagatt
    17581 tgacagttga gggaaccgta attggagata ttattgctga agaaaatcta cactttaaat
    17641 caacagcagt ttttgaagga aatattaaat acaacacact aattgttgaa aatggagcaa
    17701 atattttagg ctctctattg cataataaaa aaagtaatac tccaattagt aaagatgcaa
    17761 ctgaaaaaat cggataagaa tcaaggactc cgacagcaat tactcgcatt tagtggaatg
    17821 gcttttgaac ttttaattac tatcggaatt tttacattta taggtattca aattgataaa
    17881 agaattgcac tcaaattacc agttgcaaca gttgttatgt gtttatttgg actgattgtt
    17941 ggattctatt ttatttttaa gcaactcaaa aaattataga aaatgatgca aaaacaactt
    18001 ttgagaaagt ggttgctaac tttagtaata ttgatgttga tcggattagt tccagctttt
    18061 attcaaacaa ctttacaaaa aacttggata tattttgata cagtcaattt ttttattgct
    18121 tttatctttt gggttataat ttatttagct gaacaacaaa ctgctcaaaa gaaaatgggc
    18181 tataccctcg gagcaattgg aataaaattt atattaacca tcgttgcggt aattggatat
    18241 tttgttgtat ttaaagaaaa aacaactcca gaaattatga tagtttttat aataacaagt
    18301 atttactata tcataggcta tttttttcta tatcgattaa ctaatttgaa ataatttgct
    18361 actttgtaag tcttttactt ttggaaattt tactgtatat tttaatatat ttgccgaata
    18421 aaatccatta tgactagaaa aatttttaga atatttaaac gtgctattta tctatttttt
    18481 gtattaagta ttttaacggt cattctattt cggtttgctc caattccaat aacacctttg
    18541 atgttaattc gtagtgggga gtttttacta gaaggtaaat ctccaatttt aagatataaa
    18601 tggcgatcta gtgataaaat atcaaataat ttaaaaagag cagtcgtcgc agcagaagac
    18661 caaaagtttt atgaccatta tggttttgat ttggaagcca taaaaaaagc ggctaaaaat
    18721 aatgagaaag gcagaaagat aaaaggtgga agtacgatta gtcagcagtg tgctaaaaat
    18781 gtatttttgt ggcaaggaag aaattggatt cgcaaaggat tagaagttta ttttacagct
    18841 ttaatcgaat tgttttggag taaagaacga ataatgacgg tatatctgaa tgtcatagaa
    18901 atgggaattg gtgtctatgg tgcagaagcg gcttcggagt attattttaa taaatcagcg
    18961 acttcactta ccaaaagcga tgctgcatta atggcagcaa ttttaccaaa tccaagaaaa
    19021 tgggacgtta aaaatccatc tgggtatatt cagagaagac aaagatggat tctaagacaa
    19081 atgaattatc aataacttgt tgataattaa gtgaacaagt gtttcaaaaa ttaaataaat
    19141 ttgaactatg aaaatagtta aactcttgtt tatcgttatt ctgtgtagtt ctacttttgt
    19201 aggattttca ggaaatataa tactatatag aaaaaatgga gatgtgattc caaaggaaac
    19261 ttcccaaagt tggaatacaa atgctgataa aaaagttttt gtatattata acaatgaaga
    19321 caaatatcca tttaatacaa cgtataagat ttctatcagc aaacgtgttg gaaatatttt
    19381 tattagaacc gaaggtaaag aatttccagt acaaaaaggt caagttgaat caattacctt
    19441 ttttgagtta aaagaaccag gtgattatat tttatctgtt ttagatgaac aagggtatat
    19501 tcttagtgag caattttata ctgtccaaaa ataataacta atagctacga ttttttctct
    19561 aaaagaccta tctttgtaga taatagcgta tggtttttat tgattcatcg gttttttata
    19621 caagagcaaa aacgcattta aaatttgtct atttacattg tataaaacat tttaccataa
    19681 agaatttctt tattggtata gcttatggat taattattgg tgttattgtt gcttttgtca
    19741 atgtcctatt tagattaatt gatgaattga tgtttccaaa ctacaaagat attgaagtca
    19801 aacaacctgt ttttattata agcaacccca gaagtggaac cacatttttg catcgtttga
    19861 tgtgtttgga tgaagaaaaa tttgtatata atctactata tcataccatc ttgccttcta
    19921 ttactttatt taggatgata gatttttttg gcaaaattga taaaaaaatt ggtcgcccaa
    19981 tgcgtcgttt ttttgattat atagataata aaatgtttag tgcgtgggac gatattcatg
    20041 caacaggatt taataaaaca gaggaagatg aaggcttaca ttttgtttct ggaatttctc
    20101 caagtatagg tttgattaca ccgcatttaa aagattttaa agaactttat attcctgaca
    20161 aactagatga taaggctaat gagcagatga aaatatttta taaggcaaca attcaaagat
    20221 ggatgtatgc attaggatat gataaaatgt ttttgtgtaa aactgtaatg agtacaggcc
    20281 gtttgcagat gttacacgat atattccctg atgtgaaaat cgtgttttta cttagaaacc
    20341 cttatgaagc aattccatca tttacaagta tgtttgcaga accttggaat actttttatc
    20401 ccgaaatacc tgaaaattca tcagaatata gagaatgggg cgaggttggg attgcatatt
    20461 acaagtattt ctatgaacaa aagcaaaact ttaaacagga gaatttaatt acaattcctt
    20521 ataccgattt aataaaaaat cctaaagaca cggtactgaa aatatataac caattgcagt
    20581 tggaagttac gccaacgttc ttagaaaaat taactgctga aactcaaaaa tcaagaaaat
    20641 acagtagtaa acatgaatat agtttagaac aatatggatt tgaaaaagaa catatttata
    20701 aagaactacc aatgatattt gatgaattac atttagaaaa ataagtgtag ctttatacaa
    20761 taaaagaccg ttgcaaaagc caaattacaa aaatattcaa ggctttcatt tatttttaca
    20821 tcgcagttat catattgata attaggatta aaaataaatg aataatgaag aagattgaaa
    20881 gaattgggct tgaaatttat gaataaagat taacttgcaa cagtcttaaa aagtaaggtt
    20941 aggaatgcaa tggcgtttat ataataaaga gataatttgg gcattccttg taggattttt
    21001 tgtactcttt ttattctatg tcctaaatat agaaatcttt taccctattg gaaaaaatat
    21061 cctagatggc tctggagatg gattgaaaaa cctctataca tttggctatt atttgaaata
    21121 tgggaaagga ttatttttca atggtatgtt gtatccattt ggagaacata ttacttatgt
    21181 agatgctcaa cctttgtatg tttggttaat atggttagta gaatctattt ttcactttaa
    21241 gattgaaaat ccaataatat atattcatct aattgtgttg ttgaatatat actttgcaac
    21301 attctttgtt tttttgattt taaaacattt taaagttagt ttttttgtcg cttttgtcgg
    21361 aacagttgtg attgtcttat tatctccaca aatttatcga aatggtgacc attttgcttt
    21421 agcatcagtt gtagtaattc catttttctg gtggtggcgt ttgcaaatta ctacgagtaa
    21481 tacaaactat tttttttatg cagtaacatt aagtttagta gggtttatcc atccgtactt
    21541 gctttttatg gtcgtattgc tcctgctttc ctatgaattt gttttgttgc ttttttataa
    21601 aaataaaaga tggcattttg tttttcctat acttgcattt ttgctttttt atggcatcat
    21661 cttatataca gatacaataa ctgatagacc aaaagcagct tggggagcaa aagcatttag
    21721 ctgtaattgg tatgatttgc tattgccaat aaatggagtt gtaaaggatt tttttattag
    21781 aaaaattcca tcaataatac cattttatac tgaagggcat gcctatatta cggtatttgg
    21841 tatttttatt ttattgattt cgttttttta tcttattcga catatttata cccataaaaa
    21901 catcaagatt aaaagaggtt cgatagaatt ctggctattg gcagcaacac ctattttaat
    21961 atttgctttt tatattccat ttagatggaa tgaagaattt tttagtttta ttaatccatt
    22021 caaacaattt agaggcactg gtcgattcat ttttgtcttc tattatgttt atatggttta
    22081 tgtatttgtt tgtatggata ggctttatca caaagttcaa taccctattt ttaaaattat
    22141 tttttggttt tgttgtttga taacgatata tgatatttat aatgcttcaa attatttgaa
    22201 atatagattt gatagctatg gaaataaaaa tgcttataat tcttataaag attacaccga
    22261 taaattgact tcaaagatta aaaacaaaag tgattatgaa agtataattg tctatccacc
    22321 atctactgag ggtacagaaa aattatggat agataatgat tggaacgcaa aaatttcaag
    22381 ttattgggtt tcttatttta caggtttacc tatggtaaat gtacattctt caagagtaag
    22441 ttttgataat tgtatgcaaa tttttcaact atcaggtttt tatacgatgc caaaaccgat
    22501 tgttcaaaaa tttaatccga ataaaaagca attagtttta ttaaaaacag atcgattaaa
    22561 tgatgatgtt ccattggtac aaaatgccac atttattgat agtatcaatg atattgcatt
    22621 gttggaaatt gatttgaatt caataaatat aaataaaaat aaaagagtgt tctgggttga
    22681 ttcatcttat accttacttg aacgtaaatc ttataaaaag ggaaataaac ttggattatt
    22741 catcaatcaa acaacaaata ttttagcatt agatttgact aataaaaaat ttaataagct
    22801 tagaatttta ttttggtaca caccaaatta ttataaaact tcaagtgttc ctgtatgttc
    22861 attatattat attgatgcac aaaataagga acattttatt aaagactggc gtgaaagcca
    22921 tacagatact tataattata aagatggctg gctttgtgtg gattatgaat taaatattga
    22981 aaataatttc aagcaactta gatttatgat tgatggtaaa cagatttatt tatctgaatt
    23041 tagagtgtat ggaaaatagt tttaggtagt ttttttattt ataactatac ttaataacgc
    23101 aattgaaaac aaagtagatg taataaataa ataggtcatt ggaataatta gcatattaaa
    23161 aaagattact aaacttattt taagaatacc aattgcataa ttattcctca cttcttttga
    23221 aaatctcacg cttctataaa tatagaatag aaatattgga actattatgc tgaaaaacat
    23281 agataggtaa tgcaaaaaac ccaattgttt aatttcaaaa aactttgcaa tacctaatga
    23341 tttttttata gttgtatcat acacacctgt tcctaatcct gaattcataa aattcagata
    23401 gccttttgga gtatttaaaa atacatcaat ttgagtataa gcctgtgtga ttataaacaa
    23461 tgagatgcat aaaattatag ttgtaagcaa taactttttt agaaataatt tgtcgctata
    23521 aacataaaat aggtagccta taaaccaaaa taataatata tatctgctca ataaacataa
    23581 actaattgca atgccaatta agattggttt atttctgaat agaacataag caagaaggag
    23641 ataataacca acaatcaaag gttcttcggt taagatatac caatcctgat ttttatgatg
    23701 caaactttct attaaaaaat aagctactaa agctagaatt aagaaatcaa gcacttgtat
    23761 ttttcgctct cttttatata aaaacacaaa aataccgaat agcattacga taatatttat
    23821 ccatcttata tctattttta atgtaatagg aatgctgtat aaaatccaca ttgtaggcaa
    23881 atatattggc ttcattccat cccaaatttc atagattggg ttataaggat tttcattatg
    23941 tataaacctt tcagccataa ttttcataat tggcaacata tcagcccatt tataatttaa
    24001 aggataactg taaaagaaaa aattaatttg ataaagaaaa taaccaaaaa ttaataggag
    24061 aattgcataa taaaaatgaa tagcatatct aaattttatg acttttggtt gttctttaat
    24121 ttttaaacta agtaagaata atattccaat agaaatccca gaaataaaat aaattacgga
    24181 gcttattttt ttatactcta aaggtatttt aataatatat acagtcaaaa aaaattctat
    24241 aagtactgct ataaatagta atggcttgat gaatttatta aaacttattt gcatatagta
    24301 gctcaatgaa actaaatagc taaatatata aataatttct tagtttttta gaggagtata
    24361 agtagattga aattattatt acttctttct caaatcgggc aaaaaataag caatcaatcc
    24421 aattaaaggt aagaatgaac agataaaata gacatattct acactagtct tatctgcaag
    24481 gaagcccaaa agagcagaac ctaatccgcc cattccaaaa gcaaagccat aaaataaacc
    24541 agaaaccata cccaattttt tgggtaataa ttcttgtgca taaactaaaa ttgctggaaa
    24601 tgcagatgat aatattaatc cgattaatat aattaaaaca cctgtccatt caatgccaac
    24661 ataaggtaat acaagtgtaa gtggtgccgc tccaagtata gaaaaccaaa ttacatattt
    24721 tcgaccaaat ttgtctccaa aaaaaccacc caataaagta ccaatagcaa ccgcaaatag
    24781 aaaataaaat aaatagattt gtgcctgcac ttcagttata ccaaatttat ccatcgtata
    24841 aaattggaaa taactagtaa ttgatgccat atagaaaaat ttggaaaaga ttaggagcaa
    24901 taaaataata acagaaatac ttatttgctt ttgagttaaa tcaggtaaga caatagtttt
    24961 cttttgacca ttttgttctg cttttaatat tttactatac cattttccga tataaaataa
    25021 aatagccttt gctgttaaaa caatcaataa gaaccaaata atatagtgtt ggtcattagg
    25081 aataactatc caagctacta ataatggagc cattgccgca ccagtatttc cgccaatttg
    25141 aaaaatcgat tgtgccaaac ttcgtttgcc acccgatgcc aagaatgcaa ctcttgatga
    25201 ttcagggtga aaaattgaag agccaatgcc tactaatgta acagctacta aaatccaaag
    25261 ataactcggt gcgtaggcta aaacaataat gcccattcct gagaaaaaca tacctaaaac
    25321 ttgcgaataa ggtttgggat ttttatctgt atatgctcca actattggtt ggaaaattga
    25381 tgctgccaac tgaaaacaaa gtgtaataat accaatttgt gccatagtta aatcgtaacg
    25441 ctgttctagt tttggataca ttgcaggaat aacagactgt atcgtatcat tcaatccatg
    25501 agccattgca atagcaaata ggataggata agctgttttt gatatttgag aatttgacgt
    25561 attcatacgc ttcagacaac aaaattagga cttatgttat gaatgcttat aaagtttaca
    25621 ataagaaaaa atgatattaa ggtatttgta aagcaaatga attaagaatt caattaaata
    25681 tcaataattt agaaacaagt aattctcaga tatttcacgt atctttgcaa aaatttttta
    25741 caatgttgtt aattcaaaaa gatatggtgg taagtgtcca ctacgaactt caagtagagc
    25801 atgacggaaa catgaccgtt gctgataaat ctcaagacca gcctctagtt tttattcatg
    25861 gtgcaggaat gttattgcct gagtttgagc aaaatttagc aggtaaaaca gttggtgatt
    25921 ctgtaagttt tactatcaaa gctgaaaatg gttatggtgt gcatgatgaa aaaaatatag
    25981 cacacatacc agctgaatca ttccatgatg aaaatggaaa aatagataca gagatggtta
    26041 aagttggaaa tgtattacca atgatggata atgacggcaa ccaatttcaa ggtattgttg
    26101 ttgaagtaaa tgatgataat gttgtgatgg atttcaatca tccattagct ggaaaagatt
    26161 tgaatttttc agttactgtt gctgatgtaa gacctgcaac tgctgaagaa attgagcatg
    26221 gacacgtaca cggaccaggt ggacaccatc attaatttag aattataagt gtttaataat
    26281 aaaaagaggt tgcaatcgca acctcttttt ttatttaaag ctaaagtaaa atggattatt
    26341 aagcattcaa agcttgctct aaatcagcaa ttaaatcatc agcatcttcg acaccaatac
    26401 tcaatctgat taaagaatct acaactcctg ttttttttct ttcttccaaa ggaatagagg
    26461 cgtgtgtcat tgtagctggg tgtccaatta aagattctac accgcctaag ctttctgcca
    26521 acgaaaataa tttagttgaa gataaaactt tatctgcagc agctaatgta tctgctttca
    26581 aagaaaatga aaccattgca ccaaagcctc gcatttgttt ttttgctaat tcgtgtgaag
    26641 ggtgcgtact caatcctgga taatttactt tttcaacttt tggatgtgct actaaaaaat
    26701 ctgctatttt ttgagcattt tcacaagcac gttgtactct caaatgtaaa gttttaatgc
    26761 ctctcaaaat taggaaacaa tcttgtggac caggaactgc accacttgca ttttgaataa
    26821 aatataattc atctgctatg gctttgtctt ttaccactat cgctccatga ataacatcac
    26881 tatgtccacc taaatatttt gttgccgaat gaataacaat atccgcacct aaatctaatg
    26941 gtgtttgtag ataaggagac gcaaaagtat tgtccacaca aaccaaaata ttatgttgtt
    27001 tagcaatttt taccacagct tcaatatcta ctaaattcaa cattggattg gtaggcgttt
    27061 ctacccaaat taatttggtg tttggcgtaa tggcatcact aatattttga atcgtattca
    27121 tatcaacaaa cgttgattgg atacctaatt tgccatatat tttattaaaa atacgatagg
    27181 ttccaccata taaatcatta caagaaataa tatggtcgcc actttttaat aattttataa
    27241 tggcatcagt ggcagctaaa ccacttgcaa aagaaattcc aaaatttcca ttctctaatg
    27301 cggctaattg attttgcaat acatttctag ttggattttg agtacgtgca tattcaaaac
    27361 ctttgtgaac acctacactt tgttgaacat aagttgatgt ttgaaaaata ggtgtcataa
    27421 tcgctcctga acttatatct ggttcaattc ctgcgtggat tactttcgtg gcaaatttca
    27481 tgattaaatt actttataaa gctaaagata cgactattaa tgtttcccta taattaatta
    27541 aggtatttta ttaattgttt cattgtttta taatttcatt atggaatgac tacattttcc
    27601 tatttttgta caaaatctta ttttgagtat agcaaaaaaa ttagcatcag aaacagcagc
    27661 gtatagcttg agtagtattt tggcaaaggc tatcaattat cttttcggat tatttattgt
    27721 aaaatatatt tctactgagg agtatggtgt ttatagtaaa ctttatgctt atgctggttt
    27781 tttgttggtt tttttgacac atggaatgga aacaactttc tttagatttt tgcataaaca
    27841 aggttataaa gacaaagcct attctgttgc ctttacttca atcacgaatg cagtaatctt
    27901 tttcattgca cttgttttta ttttcattat acctatcggt aattttgtgg aggaaagtca
    27961 tcgcttgata aaaatatttg cgttcataat ggtttttgat gtgctttgtg ctttaccttt
    28021 tgcttcgttg agagcccaaa atcgcccact taaatttgca ttttataaaa ttctgaatat
    28081 tggtattttc attttcttta atggactttt gtttattggt cttccagtct tagatattga
    28141 ttttggaatc tctaaggtcg agaatatctt catagctaat cttactgcga gcatactaac
    28201 ttttatttta ttagccttcc aaactgagaa attcaaattt acttatgatg caaaattgca
    28261 taaagaattg tggatttatg gtttccctat tatgttggta ggaattgcag gcatgaccaa
    28321 tgaaatgcta gatagggcaa tgcttacaaa actactgcct tatgatgcgg caaccaataa
    28381 tattcaatta gcgatttata gttttaacta caaatttgcg atgcctatta gtatgttttt
    28441 acaagcttat cgttttgcag cagaacctat attttttaaa gaagcaagtc aggcaaacga
    28501 taggaatatg tatgccgaaa tgatgaaatt ttatattatt tgtgcttgtt ttatttttct
    28561 tggaattatg ctattcatac cagcatttca atcatttttt atttggtaca cacccaatgc
    28621 aaaagtattg tttgaaggag tctttatggt gccaatttta ttattagcca atttatgttt
    28681 aggtatttat tttaatattt ctacttggta taaaattacg gacaaaacac attttggtgc
    28741 aattatttca ggtattggtg ctgtgataac aattgtatta aatctaattt tagtacctgt
    28801 ttggggatat gccggagctg cttgggcaac tttatttgtt tatattatta tggttgctat
    28861 tggttttact ttggagcgaa aatattttcc aataccgtat caatatggaa gaattggttt
    28921 ttatctatta gcgtcattat ttctttttgt aatatatact tattttatcg caaataatat
    28981 tgaatcactt cttataaaaa gtgcaattgc tttattaata ttaggcattt atgttatatt
    29041 agtatataag actgaaaaaa gaaaataact tttatctttg ttttatatct ttactcttta
    29101 aataattagg atgatgaaaa aaatgatttt aaaaatagtc aatgaatcgg gaaatccact
    29161 gccagaatat gaaacggtag gttcggcagg tatggattta agagcaaatt tgactgagcc
    29221 aattatgcta aaaccactcc aaagagcatt aattccaact ggattattta tagaactaga
    29281 acaaggatac gaagctcaaa ttcgaccaag aagtggattg gcactcaaga aagggttgac
    29341 aattttaaat tctccaggca ctattgattc tgattatcgt ggtgagataa aaattatcat
    29401 tattaattta tctaatgaaa caacagttat caatactggt gaacgtattg ctcaaatggt
    29461 gattgcaaaa tacgaacaag caacattaaa agaggtcgat gaattgggtg aaacagagag
    29521 aggtgaaggt ggatttggac atactggaat catctaattg taaattatta ctgaaatact
    29581 atctattaaa ttttttagct taaaatgcat atattagtta tcaattgtgg cagttcttct
    29641 ttaaaagctg acatcatcaa tacaatcaat aaagaaactg ttgcagaact tagagtagag
    29701 agaataagca ctacacctga aatcaaaatt aatcaaaacg aaattgctta tactggtata
    29761 gttaataccg aagagattat tcgattttca ttaatacaat taaaacaatt tgatactaat
    29821 atacagatta gtgcaattgg acatcgtgtc gttcatggtg gagataaata cacacagcct
    29881 attttagtta atgaggatgt ggagcttcaa atcgaacaat tgataagttt agcaccacta
    29941 cacaatcctt caaatttaag tggtattcgt atttgcaaac aagtttttga gggattgcca
    30001 caagttgcag tttttgatac cgctttccat caaacaatac ccaatagagc aaaacattat
    30061 gcgatagata aaactattgc agaaaaatat ggcattagaa ggtatggatt tcatggtact
    30121 tctttaaaat atgtaagtga gcgagttgct gaatatttac aaacggatat taaacatttg
    30181 agaatggtag cttgccattt gggaaatggt gcaagtattt gtgcagtaga atttggacga
    30241 agtgctgaaa cctcaatggg aatgacacct ttagaaggtt tggtaatggg tacacgctct
    30301 ggagatattg acccagggat tatactttct cttatccatg aacataaaat gagtgttgaa
    30361 gaagtggagg aattattaaa taaaaattct ggattaagtg gtttgagcgg tataggaaac
    30421 gatatgagag atattttgaa gcaagctgag gaaggaaacg aagattgtag aatggctatt
    30481 caagtttata cgcatagatt gactaaatat attggtgctt atgttgcact aatgggtggt
    30541 gttgatgtct tattatttac tggagggata ggagaaaatg ctgctacaat tagaaaaaga
    30601 gtttgtaata aactcaattt tatgggaatt gtcctagatg aagataaaaa tatctatcaa
    30661 aaactaacga atgaacaaga tgttattgag ttgcaagata gtatatcaag agtaaaaatt
    30721 gttgctgtaa aaaccaatga agaattagca attgccttag atacagaaaa gatagtttca
    30781 gaaaagaata aagtaaatac aattccaaaa attccaattg caatttctgc acgacatatc
    30841 catcttaaac aagcaaccat agatttgtta tttgggaaag gttatcaatt gactgaatat
    30901 aaaccgctgt cgcaaccagg gcaatatgca gctaatgaaa aattgacttt agtaggtcct
    30961 aaaaataaaa ttgagaatgt acgaatttta ggtccggcaa gaaattacga ccaagtagaa
    31021 atctccagaa ctgatgagtt tttcttaggc attgatgctc caattcgtga aagtggaaac
    31081 attgcaggaa gcccaggtat tattctagaa ggtccaaacg gcacagtaca aattcatgaa
    31141 ggagttatac aagcatggcg acatattcac atgcatccag atgatgctgc aatgtttggt
    31201 gtgcatgata aagatattgt gagtgttgat gtggaagata aagacagacc tcttactttt
    31261 aaaaatgtat taattcgtgt gtccgataaa ttcaaattag aaatgcatat cgataccgat
    31321 gaaggtaatg ctgcacaaat tcattctggc gaagagggcg ttttggaatt aggaaatgta
    31381 agttgtcagt tacacgaaaa gaatatttaa ttcagttgtt cccaagtttt tttggctaat
    31441 tggtacgtta aatattcaat ataataggtg cctttaacca catcaggaat ttgtttgaaa
    31501 ttgctttcat aatagagtaa atgatgaatg tttcgagcaa tacgtctgcc tagattggaa
    31561 ttttcgtgtg gatgaatgat taatgcatca caaccaccta aaatggcact catagcttca
    31621 gtggtatttc ttagaatatt gttttctata aaatcatcgt ttctattttc aaaaccagtt
    31681 tctgaaaaaa gaacaaattg cttgttttta atttggagtt gatttatttg agccactaac
    31741 caacgaaaag ccctgatttt tgcaatttct aagaaataat tttgcccaat attgaagtgt
    31801 acattactga tttcagcttc attttgaaat aaataatgaa cggcaaaaag taattcgttt
    31861 acagtacttt tttgttgtgg aacgaatatt tttggattgt aatttataga atattcagaa
    31921 agtaggtgaa cagaagtatt gccaatattt ttagcataaa tggtaattgc atctaataga
    31981 aaatctttaa caaggatagc tatttgctct tgattaaagg cattattctt aaaattaaat
    32041 aaaattgtat ttgctccatt ttgtaatgca ttcagagctt cttgattggc tttttcacaa
    32101 ttttcaacag taatatattc tgtaatttgc caatcttgtg tttttcttgt aggaatgttt
    32161 aatatataat cctttgttgt ttcggcagta tatataggca acatttctat accatcttca
    32221 gttaatgtaa ttaaatcttc aaaaactttt ccttttaaat ctgaaataat tttctctttc
    32281 caagtcggaa aatcagtacc attaaataaa tctaagacgg tgtcaaatcg ttgttcaaac
    32341 atattgcaaa tttcagtaaa ataaattaaa ctaccaatta ctctacgatt ttctcgcctt
    32401 agataatttc aggagcatta acgacattta ctcttgcaaa attttgggtt ggaaaaataa
    32461 ctttataatc tgtccaacct ttttgtccag ctctcgaatc tggcaagtaa taatctgtag
    32521 aaataatttg tgcaccacta ttgaaagcat cttctctagt ttttgtattt cctgttctag
    32581 cttcttctgt atcagaatca gatcgagttc ttataattgc accagctttt acagcagctt
    32641 gtattttact gacctcctca ctaccgtcca tttttacaaa tgctccatta tctgaaccaa
    32701 gcggagaaaa ttggaacatc attctacctt ctaaattcgg atgatttttg gtatataaat
    32761 tattagcata aaagacaaaa attattttcc ctcgcatagt tgaaattttt ggccaacctt
    32821 cttgctgtat cgcagttttt aagttgggat atgtttttct taaatcatca ggtctgaaaa
    32881 tttgtgatga atttgcacca aaaacatcaa taatttcctg atctacatca ttaaccgctt
    32941 gctctgtgaa aggtaatatt tttgtaaaaa atggcaatac attacccacg ccaaattctt
    33001 tatattctac taataaaaca ataggtaaat gtgaaggatt ttgattgctc cattctttta
    33061 tggcttttaa accagatttg aaagtataat aatgcgtatt ataatccaca tcaggaatat
    33121 gcatcatctt cataccaggt tgcattaact ctggcactct cgaatcaaca cttttaccca
    33181 ctaatgcgtt gccaattcgt ttgtaatatc gaccaccagc aggatcgtaa taaatatcta
    33241 attctaaact tcgtacacct aagtcaaatt gctcattaaa aggtatatgt gtataatccc
    33301 aagcttttgg attcaaatca ctgggtaaaa taccaccaat agcagatacc aatttataaa
    33361 tatcaggatc tgttcttttt ctataactat tgtgtgaacc tatgcattgt atttgattaa
    33421 attttaaagt atctaattgt ggatttatct gttctgaact ctttttaatc cgttggttgg
    33481 tttctttttt acaagagaaa atgcaaaatg aaatacaaat aaatagaatg aaatttctca
    33541 taattacaat tagttaccta aatgtaggta aaaaatcaga aaaataataa aattatcaaa
    33601 agataattta cttagcaatt tttacttgat aagttctacg ttctttattg taaagttttg
    33661 gatcgcgaat ccaaggattc atgcggcgta tcattttgta agaggtattg ttgttttgag
    33721 caaaagttac ccaatctaca tatccatcga cagctattgt ttctctgata ggatatggtt
    33781 catataaatc atctttatct aaataaaaac catatttctg ttgattttca tagattactt
    33841 ttaaagctaa aattctaaag atatatctac tggtttcact atttaaaaat aaattataat
    33901 aatcactatc gccttgctta ttcgcttgat tattaattcc attgatgccc atattataac
    33961 ctgctgctgc atttgtccaa gagccatttg atgcttttgc actggttaaa tatttacaag
    34021 ccgcaatagt tgatttttca atatcgtacc tttcatcaac ttcagaattg actactaatc
    34081 cataaattgg agcagtgctt ttcataaatt gccaatagcc agatgctcca gcagaagaaa
    34141 ctacattttc caatccactt tcagccatgc ataagtactt gaaatcatcg ggaatattat
    34201 tttctgctaa tattttctct ataattggaa aaaatcggtt ggctcttttt atcattaaaa
    34261 tagttgcaga atgtttgtaa gaatttacaa tcagttcatt gtctaaacgc tctctaattt
    34321 caatatcgtc taaaggcact ttttcgccag caaaggatag ggttgccgga atttttggtg
    34381 cattcacatg ttggtttggt aaagatgttg tggtggtttg agaagcattt gggttaaaaa
    34441 aagtaaatag accatcaggc gaactcgctg ctctgacaaa taataatgtc aataatgatg
    34501 aacaaataac ggtggcgata aaatacaata ttcttttcat agatttttat tttttctttt
    34561 tatacactgg cactgttgaa caaggttcgc caaaaaacaa actagttaca tgtggtatta
    34621 acttcttact aatttctaca taagctgcac ttgataaagt ttcttgacta catcctttta
    34681 taactacttt ttgatttata aacgattcaa tatttatttt attgatatta tctaataaca
    34741 aattttgtgc tacaattttt ggattgccat aatatatttt atttgtaaaa ggtaaaagat
    34801 attgtgtaat caacataaaa ctccattggg gtaaaattgc atctgatgaa caaaatatag
    34861 caatagtctt gtctgaaaat tctacccaat tttgtgcttt taaagctaat ctaaagtctt
    34921 tctctttaag aatcagttct ttaaataata aaggtttgat atcaaatgct attatggctt
    34981 cctttggaaa ataatcttcc aaatctattg tgattaaacc actttcagca attttattct
    35041 ttatttccat catttttgct aaaataatat ttattctttg taaaagatgt taattaagca
    35101 aaattgaaca aactctatat tttcaatatc gttagaataa atgcttaaac caattgaaag
    35161 attttggaaa caagagcaat gcaattggaa taaagattag aaaataaact tgatttaatg
    35221 ccagaacgaa gataagtata tagatgaaaa ataattcgta atatagattg acctctctga
    35281 gtatatttgt agaaataata gattttcgtc ttactaagaa cggtataata tattgttgtc
    35341 tttggtggat ataaattaag aaaatgatat agctactaat gaaaataaaa ttatatggta
    35401 gttgtaataa taatgtaaga aagctaataa tttccaaagg caataaatat atctttaaga
    35461 aatgcatgat tttttctttg tcataataag tagctattgt tttgaaatct aattgtttat
    35521 caaagttggc atcactgatt aaatgcaaca aaaaatttct taaaccacgt acaaatgccc
    35581 ataatgataa agatgctaaa agtagataat taagtttatt tgagtataag catatcgcaa
    35641 gaatatagaa cagtgttcca ctatataaag catcaagaat taaagctaaa attttatacc
    35701 gtttaaaacg aaatggtttt agagaataaa gcaataataa gctgatttga attgcataaa
    35761 gtaaaatact ttttagattc catgtcatca caaataatgt gagtgtaatt cctaataatg
    35821 taccacttaa taaaattact ttataagccg aactactgtc agccagatga ttatgttttt
    35881 gagctatttt atcatcagcg atatcactcc attcattgat aataaaccct aaagcagcag
    35941 taaaaactgt tattcctaaa aagtatagaa aatttaaagt tgtgtttttt aaggtatgat
    36001 tttgtggaga tgaaagtagt aaggcaacaa aataaaatgc catagttggt ggaataatat
    36061 agtcccacca atccttaatc ctaatatgtt tactaattga cttgacttct attctatgaa
    36121 ttcataaata tattcagctt tatagaatag aacgaagtcg tcttaaaaag agataggttt
    36181 tgaatcataa atttgataaa ttgttgattt taaaggaatt ttttagtaca aaataattta
    36241 attttaactt ttatattaat acataattta gtaacttcgt tgcttaacta aaaaagtgcc
    36301 atggtaactg atttcaatgt aaataatgat gcagtagaac aaaaagaaga agttagagta
    36361 tttgaagaat atattgaacg aaaaccaagt tcattatttg taagatattt tattacccaa
    36421 atgcatctta gcggattatt attgggtgga ggctttgcct atcttcgtca tagaaaagaa
    36481 accaaagcaa aatttagttt tgggaaagat gttttcctta gattgttttt atggttgcat
    36541 tggatttttt taaataaaaa acttatcaag cagccattta aagtccaatt gagaagaaga
    36601 ttggaaatgc ttggtgcaac gtacatcaaa ctcggtcaga ttatgagttt gagagaagat
    36661 gttttaccag tggaaattac agatgaatta aaaaagttat tagatagttt acctgctgta
    36721 aagtatgaac gctttgctga attagttgaa gagaatattc aacaaccgat tcatcgagtt
    36781 tttgttgata taagcactaa tccattaggt tcagcatcca ttgcacaaac acataaagca
    36841 acattaaaaa ctggagagga agtggttatt aaacttctaa aacctggaac tcgtgagttg
    36901 attaaaacgg atagtaagat tattaaattc atgggtcgtg ttctgaattt atttgtgcca
    36961 gagttacagc caaaaaatat gtttactgag ttttgtgatt ataccaacaa agaagtagat
    37021 tttagattgg aagcagaaaa tgctgaagaa tttgcaggta attttacaaa acatccagat
    37081 attgtttttc cgattattta taaagaatat tccaatgaaa atatgattgt catggaatta
    37141 tttaagggta ttaaacctga taaaaaagcc tacgaaaaat ataatttaca agaacgtgaa
    37201 agaattgtag atattggtgc ttatgccatt atacaaatgc tttataaaga tggctttttt
    37261 cacgctgatt tacatccagg caacttaatg ataatggacg gtccgaatgg cccaatgtgt
    37321 gggtttattg atttgggtat ggttggaaga tttgaggaaa ccaccagaaa agcaatgctt
    37381 tattatttca acagcttagt gatgggcgac ccagagaatg cggctagata tttatcggcg
    37441 ttagcaaaaa cagataaata tagcgattta gatggtttta gaagagaatt tattggtgtt
    37501 ggtaataagt ggttgaagaa ttcaaatttc aaagatttct ctttggcaaa attaattatg
    37561 gagtctgttg tattgggagc aaaatatcgt ttgtattatc ctttggaatt ggtattgatg
    37621 gttaaagcaa ttatcactta cgaaggtgta ggaaatgtaa ttttaccagg atttgatata
    37681 cagaaagttt caaaaaaaca tattagaaca atggttttag gagaagttaa tccaatggat
    37741 atgctccgtc agcagttgtt aaatgcacct gaaataatgg atgtaattat caaagctcca
    37801 atgctaatta ctgaaagtgt aaaacgatat gaaagtagat tagtagagaa aaaaggcaaa
    37861 tcaggttctg aatcgataaa aacaatgatt tttgcaagtt gctgtattct tggtgcaagt
    37921 atgatggcat ctgctggttt agaatggttt acttttgttc cattattcat tataggtggc
    37981 attattgcat taaaatcata aggaataaac ctttacagca agttttattt ttgtagcttt
    38041 gtactgataa ggatatattt ttggcaaaga ctataaaaca taaaatcgat ggtattgact
    38101 tcaattttca tatttgggga atcaatagtc ctttagttga ttatagattg tgttggttac
    38161 taaataatct tttagattgg aattttgaaa gagttgatga tatagaaatt agttcagaga
    38221 aagatgattc tataatctat tttaatgcct ataaatacga aaacaaagaa gacttttata
    38281 cggtagaaat tattcaaaat aaaaatgatg gttttgtact tttgccagaa ttgagaaaca
    38341 ttgattactt atttatattt gatggcgaag aagattattt tgataaagat gaaataacac
    38401 caatatttca acaaattcaa ggaatacaat ctatttttga aattgaagtt gataatctca
    38461 aatcaaagta taaattatta atgagacatt ttaatgatac aaaataatag aaaaaccaag
    38521 atagttgcca cagttggccc agcaagtaat tccttagaaa atttagaaaa gctttattta
    38581 gctggagtca atgttttcag attaaatttt tctcatggaa cacaacaaca gcatcttttg
    38641 gtaattcaaa atattaaaaa aatcaatcaa aagcataaaa gcaatattgg aattttagca
    38701 gatttgcaag gtccaaaatt gcgtgttgat gcaatacaaa ataatgcaat tttattagaa
    38761 gaaggaagaa atattatcgt ttctactgag gaagttttgg gtacaaatga aatgattagt
    38821 gttacatatc cacatctttc tcaggatata aaattaggcg aatcgatttt aattgatgat
    38881 ggaaaaattg aattaaaagt tacgaaaata attaataaaa ctcagataga atgtgtgatt
    38941 gaaaatggtg gtattttgtc ttcaaaaaag ggatttaatt tgccagatac agaaatttct
    39001 attccaagtt tgaccgaaaa agatatcgca gatttagatt ttatcataca acatcatgtt
    39061 gattgggtcg cactttcatt tgtgagaaaa tctgaagata ttatagattt aagaaaaaga
    39121 attgcggcac agcacggcac tcaaaaaatt atcgcaaaga ttgaaaaacc tgaagctatt
    39181 aagaatattg atagtattat tcatgattct gatgcaataa tgattgctag gggcgatttg
    39241 gctgtggaag ttccgatgga agaaatgccc attattcaaa aaatgattat tgaaaaatgt
    39301 atcgaccatg ctcgtccagt tattattgcc acacaagtta tggaatctat gattgaggca
    39361 ccacgaccta cacgtgctga aattacggat attgcgaatg gtgtttttga tggagcagat
    39421 gcaatgatgt tgagtgcaga aacatcagtt ggaaaatatc ctgttaaagt cattcaagca
    39481 atggataaaa ttattctaaa aactgaagag cagaataaaa tttattttaa aaacctttct
    39541 cctaaaaagg attctccaac tttcttgtcg gatgctgttt gctataatgc ttgtagtatc
    39601 gctgaagatg ttgaagcgac agcacttttg ggcatgactt attctggtta tacggctttt
    39661 atgttggcaa gttatagacc aaaatctaaa atttttattt tcactagcaa tcctcaatta
    39721 atttatcaat tgagtttggt ttggggtgtt gaagtgtttt tctatgataa ttttgaaaca
    39781 acagataaat ctattcgaga tattattggc gttttgaaag atgcaggaaa aatacaacaa
    39841 gacgatattg taatcaatac agcaagtatg cctatcaaag gaagaggcag aaccaatatg
    39901 attaaaatta cgattgtaga ttaattttat tacaatctac aatcaaacaa ttttaaagtg
    39961 atttgaatat ttagttttta gatttttatt ttgatcttgt aaccaaatta aaggcatttt
    40021 tcgtggaact tttaccatat caactttatg gtaagttcca aaaattctat cccaaaatgc
    40081 agttgttaca ccgtgattga attttggatt tccaaagtga tgataaaaat gatgttttcg
    40141 cattcgcaaa ccatatctga ttattggttc ttttatatga aataatttat gtgcggtttc
    40201 atatataaaa tacatacttg ccaatccaat tgaaaataag aaaccatctt taagattaaa
    40261 aataagccca gttattaaag ttgagctaaa taatacaaca attgctaaac ctaacttttt
    40321 ggctattgga acaaaataat tgacttcagc atgatgtctt tgatgttctt ttttgatgat
    40381 atgctttcca ttcttttgat gtcctaaaaa acgatgaatg atatattcaa ggaatgtcca
    40441 acttataatt ccaatggcta aaaataatat tgagttcagc ataaaatatt ttttattgaa
    40501 gttaatctat atttttttaa ttgtataagt tgctgatagt taataagaaa atttaactaa
    40561 ataaaattcg gctacttttt tagcgctttc ttttctattt tctttttgct attacctaat
    40621 aaataatcat aaattttata ttgacttcta atgcttgttt tttcatcaat atatctaaat
    40681 tgattggttt gaataccgct aataattctt gcttttacat tatcctgcca ttgaatatct
    40741 ataatattag caagtgtttg ttgtattttc ttatctataa taggcgtaat gacttctact
    40801 cgataatcta aatttcttgc cattaaatcg gctgagccaa taaaatattc tggttcattg
    40861 ttgttgtgaa aaataaaaac ccttgtatgt tctaaaaatc tatcaactat actgattact
    40921 tcaatatttt cactcagttt gggcaattgt ggaattagtg aacaaatacc tctaacaatc
    40981 aattttactt taacaccatt tttacttgct tgatataacc tacgtatcat gtcttcatcg
    41041 gtaagactat taatttttat agttatagaa gcagttaagc ccattttggc aaattttatt
    41101 tctctattga tgagtttttc taactttgtt ctcaaattaa aaggagcaac caacaagcga
    41161 ttaaattttg gagcgtaata gtttttatct aaaaaagtaa ataaatttct tacttctttt
    41221 gttagtaatt ggtttgaagt aagcaacatt gcatctgtat aaatgttggc agttttttcg
    41281 tgaaagtttc cagtaccaat tgcagcataa tatatttttt gacctctttc tcttcgagtt
    41341 attaggcata atttagaatg tacttttaaa tcaggaatac cagtgataac tttagcacct
    41401 tctttggtta agatatttgc ccattcaata tttgcttctt catcaaatct ggctctcaat
    41461 tcaataaaaa cagtaacatc tttaccattc ctcactgcgg taatcaatga ttttacaaca
    41521 ttggagttct tagctatacg atacaaagta catcgaatat gtgaaacact tgggtcaatc
    41581 gctgcttctc ttaataaatc taaaaaataa tcaaatgaat gatatgggta atacaacaat
    41641 atatctttct ctttaatttt tgaaaggata ctatgcacat ttttcaaatc agcatgaggt
    41701 atttgaggta gataatcata acgtaaatga tttctgttaa gtgttggaaa tttgataaaa
    41761 tctttgaaat tatgatagcg agctccagca acaacatgtg cctctttatc taaatgcatt
    41821 ttcttaatta gaaaattcag aaaatctttt ggtattttag catcatgaat gaaacgcaaa
    41881 gcatctgcat tttttctttt ctccaaactt aattcaacag attctaaaaa gcttactgca
    41941 acatcattat cgatatccaa ttcagcatct ctagtgaatt tgatggtata agcttcaata
    42001 atatcaaaat catatagttt gaaaattgat tttaaattaa aacgaataac atcatctaaa
    42061 aatataatat atttcttatc atcttctgaa ggaatttcaa caaaacgagg aatgctattg
    42121 gttggaattt caataaggga ataatgtttg gttttggctt tctttttata taattgaaca
    42181 gccaaataga tggaggcatc ttgtaaaatt ggaaatgatt tatcatttct aatcataata
    42241 ggaaaaactt ctaatcgaac tttgtcgtga aaatattgtc taacaaaatt tccttgttgt
    42301 ttacttagct ctttttcatt tacaaaaata atgttttcct tttctaaatc tgctaagata
    42361 tttgtatagg cattatcgta tcgttcttgt aaaacaagaa tttgcttttg catttcatct
    42421 aataatttaa taggattgaa gcccattttg ttttttgcct gttttttaaa atccgtcatt
    42481 ctgcttatgg tagcgacacg aactctaaaa aattcatcta aattatttga ataaatacct
    42541 aaaaatttta aacgttcaag taatggaacg gctgggttct ctgcttcttg cagcactctt
    42601 tcattgaacg aaagccaact caaatcccga ttaatgattg ttaatttttc catctaagtc
    42661 aaagatattc taatttaatt aaaagaaagt gattgatatt gtaaaaaatt ataaattcaa
    42721 ttgtttaata tagttttaaa taaaaataaa attggaatag aaaatataaa aatagtaact
    42781 ttgagttatg tcaacagcga aatctgaaat taaaagagta actacgcaca agcttaaaga
    42841 aatgaagcaa cgtggcgaaa aaatatctat gttaactgcc tatgattttt ctatggcaaa
    42901 aatattagat gatgcaggaa tagatatctt attagtaggc gattctgctt ccaatgtaat
    42961 ggctggccac gaaacgacct tgcctattac tttagatcaa atgatttacc atgctcaaag
    43021 tgttgtacga gctgcaaaac gttgctttgt tgtggtcgat ttaccttttg gttcttacca
    43081 aggaaattct agagaagcct tgaatgccgc cattcgtgtg atgaaagaaa caggtggtca
    43141 tggtgtgaaa ttggaaggtg gacaagaaat tggagaatct gttatcagaa ttttgactgc
    43201 tggtattcca gtaatgggac atttgggttt aacgcctcag tctatttata aatttggaac
    43261 ttatactgtt cgtgcaaaag aagaaaatga agcagaaaga ttaattcatg atgcaaaata
    43321 tttagaaagt attggctgtt ttgctattgt cttagaaaaa attcctgccg atttaggtaa
    43381 acaagtagca gaggcattaa gtattccagt tattggaatt ggtgctggta tgcatgttga
    43441 tggacaagtt ttggtaacgc acgatatgtt gggtattacc gcagaattta aaccaagatt
    43501 tttacgtcaa tatctacatt tatacgatga cataaaaaat gcaacggtac aatatattgc
    43561 agacataaaa gcaaaagatt ttccgaacga gaaagaacaa tattaaatca agtatttgta
    43621 ttaataccaa aatgcaaacc ttgtattaaa aaataattta ttctaaggtc taattgttta
    43681 ttgttgaaat aaacatttac ctttgcatta aatgttctta ttcatcaatg attaatcgta
    43741 gaagtgtccg tgtcaaaacc atgcaggctt tgtatgcatt tcaaagttct gatactcaaa
    43801 gtaccaatga ttttgagcaa tatcttatca aaagcatcca atctgttaaa gaacaatacc
    43861 tttttatttt attatacatt cgtgaaattg caaattatgt tgagcaagat gcacacatta
    43921 aagcaaagaa gtatattcaa acagaaaaag ataagcaatt tagtacaaaa ttattaagta
    43981 atacttttgt tcaatatttg aatcataatg aagaatttca aaatgaaatc aagaaatcaa
    44041 atacacaatt attaattaat gtagatgaag taaggaagtt ttataagaaa ttcagcgaaa
    44101 cagcagctta taaaagttat cttcaaaatg aatcagaatt caatattgat gaagaccaaa
    44161 aaattatagc attccttttc aataacgtat tattagaaga tgaacatttc ttagaaatta
    44221 ttgaagagtt ttttatcaat tggaatgatg atgctctatt ggtagtagat gctgttcgtg
    44281 aagcgatcaa gaaaagtaaa aatgaattag ctttacatat tggcaagcaa aatgtaaaat
    44341 ctaaaattga tgaattgact aaatttggaa aagatttatt tttcaatgtt gttcataaaa
    44401 aggaagaata tattggtttg atagaaccaa aattaaaaaa ttgggaaacc gatagattag
    44461 ctatgttaga tgtccttatt ttgagaatgg cattgtctga atttatagat ttccctagca
    44521 ttccagtaaa agttactatg aatgaatact tggatattgc acgagaatat tcaacatcaa
    44581 agagcaaaga ttttatcaat ggcgttttag ataaaattat gcaagagttg aaggcaaaaa
    44641 atcaactcaa taaaactgga cgaggtttat tataatttca aaatatccat attgtaactt
    44701 acagattcaa atttcgtttc ttagttgctt tatttttgat atatttgttg ctaattatat
    44761 agaaaatgaa gattacagaa atcctagagg ctgaaaaagg caaaaattta atttcattcg
    44821 agatattacc acctgtaaaa ggtaaaagta tagaggcaat tactaaaatt ttagatccat
    44881 taatggaatt taagccatca tttattgatg ttacttatca tagagaagat tataaataca
    44941 aacaaacggc agctggttat tttgaaaaaa tctctatcag aaaaagacca ggaacagtag
    45001 ggatttgtgc tgcaatcaaa tatagatata atatcgaagc ggttccccat tttttgtgtg
    45061 gaggatttac taaggaagag actgaaaatg ctttgataga tttagcgttt ttaggaatta
    45121 ataatatttt ggcattacgt ggcgatgccc gtggtatgga gaaaaaattt attcctgaag
    45181 caaatggaca tgcttatgcc gtggatttaa ttagacaaat tgaaggaatg aactccggta
    45241 tttatttaga tgatgattta gtaaatcctg tacatacaga tttttgtatt ggcgttgcag
    45301 gatatcctga aaaacatttt gaagctgcaa atttggaaga agatttacaa tatctaaaag
    45361 ataaaattga tgcgggtggc gattatatcg taacgcaaat gttctttaac aatgaggctt
    45421 tctttaaatt tgttgaagat tgtcgaaaaa taggtattac agttccaata attccaggtt
    45481 tgaaagcaat tacaagaaaa tcccaattaa attctattcc atcagcattt tatattgact
    45541 tgcctgatgc attagtgaaa gaattgcgaa atgctaaaga tgatattcaa gccaaacaaa
    45601 ttgggataga atggtgtatt cagcaaagta aagagttgtt ggcttatggt gtgccttgtt
    45661 tacattacta tacaatgggt gatgtgaaaa caacttcagc tatttgttct ggtgttgttt
    45721 aattgaacat tttaatagca cagcatcaat tccaaaaagt tctgcttgtt taaaatcttt
    45781 tgattttatt ccatatataa actctgtaat ttcaatgttt tttggaatac taaaagtttg
    45841 ctttttatgc ttaaaattga taaggataag taagctttct gtgtcttttg ttcgagtata
    45901 agccaagata ttatcattat ttacatcaac gatttcaaga tttccatttt gtaacgaatt
    45961 actatttttt cttatggcta ataagttttt atatgtataa tagatactgt ttttatcttg
    46021 ttgtatcgtt tcaacattac aagtttcaaa atcttcatga acaggcaacc aagttgtatc
    46081 agcagttgaa aatcctgcat tttttgtttt atcccattgc attggagtcc tacaattatc
    46141 tcgattgata ggaactggca aggcatcaac aattatttgt ggtatccatt tgaaagtttt
    46201 ggacatacca tcttgagcgt tttttagagg tattttttga ttgaccattc caatttcctc
    46261 gccataatag gatgtaggaa cagctcgtgc agttaattgt aaaagtgtta ccaatttagc
    46321 cttttcagga ttatttccaa ctcttctaat acttctaaat tgatcgtggt tggagaatac
    46381 caaagtgggt tttaaaggag ctggatattc cctttcatat tctaaaaatt tcttcctaaa
    46441 aaatgaggca ttgaatttat aaaagcaaat atcaaataag aaaatgagat gcaaaccgtt
    46501 gtttttgcct aaaaattgtt tctttaaact atgtaaacca aaaacttcac ctagaagcat
    46561 tctttctggt gtgaattcgt cagatacttg acgcaattct tttgcaaatt caaaatttcc
    46621 agcttgattg acattgtatt ttctaatttg aaaattgcca ccaggataat cgtgtgatgg
    46681 aattgcacga attaatgaaa atggattatc tctaaaatcc ttatctttta taatgcaatt
    46741 aaatatatct aatcgaaaac catctacacc ttttgctaac caaaatctgc aaatatcaaa
    46801 cattgttttt ttgacttcct catttctata attcaaatca ggttgaaaat ttaaaaagga
    46861 ggaataaaaa tattgcttac gttcaggagc atatttccaa gcttttgggc caacaatact
    46921 tttccaattg ttaggtttgt ctcgccaaat ataccaatcc gctttaggat tcgttttgtc
    46981 ttttcgagat tctaaaaacc aaggatgttg gtcggaagta tggttcataa ccatatcaaa
    47041 aacaattttt agcttacgtt tgtggcattc gtctatcaat tgttgagcat cttctaatgt
    47101 accatattct ggagcaatat ttgtataatc agcaatgtca tatccaaaat ctacttgtgg
    47161 tgaagtataa aaaggagaaa tccaaatagt ttcaaatccc aaatctttaa tataatctaa
    47221 ttttttgata atgccttgta tatcgccaat tccatcgttg ttgctgtcaa aaaaagaacg
    47281 tggataaatt tgataaattg tagtcgtgta aatccaattt tgattcattt tttaaaaata
    47341 aggaatactt tcagatataa caatgattaa acccaacttt aattgaatga actataataa
    47401 tgctttctaa ttcttaaata atatgaaata gttggtattt tgaaggcttt cttttataat
    47461 ttaatgatat ttagatagtt catttctata tttgtagttt aattctaaat ttatatatga
    47521 gaaaagtttt taagggattt tttattttcc tattcgtact aatattgagt gcatttgctt
    47581 tgcctttctt ttttaaagat aaaattgttc aattagccta caaagaaatt gaaaaaagaa
    47641 ttgatgcaga tgtaaatatt ggcgatgcag acttatctat cttgaaaaat attagcaatt
    47701 ttccaaatat tgctttggct ttagaagatg taactttagt tgggaaagga gcttttgcag
    47761 gagatacttt gttgaattta ggaaatttaa aaacatcatt agacttaatg agtgtcatca
    47821 aaggagatga atataaaatt gaagccattg agttgaaaga tgtttatgta gatgcaatcg
    47881 taaataaagc aggaatgcaa aattgggata ttttaaaaga aacaccaacc gatacttcaa
    47941 cagcttctcc attcaaatta gctttaaaga aatttatctt agataatgta aatttagaat
    48001 ataatgattt tacaaatggt gatgaattaa agattgaaaa tatagaacac caagggaaag
    48061 gtgattttag tacagaagta tttgactata tttcaaaaac tagcattgaa aaactatcct
    48121 taaaacaagg acttataagt tatttaaaga attcaaaact aaattttgat agtaaaataa
    48181 atatcaacca aacagaaaat aaatatagtt ttaaagatgc aaaattgacc ttgaatgatt
    48241 taggattgtt gtttaatggc tttgttcaaa cttttgatga taaaacaaat ttagatgtac
    48301 aatttaaagc agataaaaca tcttttaaat ctattttgtc tatgattcct gctatttata
    48361 ctaaagactt tgataaagta aaagcttctg gaaacttagc tttagatggt agtattaaag
    48421 gatttttaca aggcgaaaac tatccaaatt tcaacctaaa cttaaaagtt gataatggaa
    48481 aattccagta tccagatttg ccaacttcgg tgaatgacgt ttttattaat gcaaatattg
    48541 cacatgcaca aggcaatctt gataattcaa ttatagattt gtcaaaatta gattttaaaa
    48601 tgggtactga acctattact gctagattaa aagttttaac accaatttct gacccaaatg
    48661 tagatttaat ggcaaaagga aaattagact tggcaaatat catgaaaatt tatcctgttg
    48721 aagatataga gcaattaagt ggaaatgtaa atgttgattt agctgtaaaa gcaaaaaaat
    48781 cggatataga taaaaagaat tataccgcaa ttactgcact cggttccatt gtttctaata
    48841 atattttatt caaatctaaa gaagtaaaag atgccgtttc tatatcttct ttgttgctta
    48901 atttctctcc tcaatttgtt gctttagata attttaaagc caatctcttt gaatctgact
    48961 ttgatatgaa aggaagattg gataacctta ttggttattt tctttcaaaa gatgcgatac
    49021 tttctggaaa tttgaacctt gtttctcaac aaattaatgc caataaattt ttgcctgact
    49081 caacaagtgc aaatcaatca aaggcacaac aagcgaaaga tgttgttcga ttacctaaaa
    49141 atattgactt cacaacaaat actcaaattg gaaaattact ttatgataaa ttagtcatag
    49201 aaaatctatc aggaaatatg aatctgaaag atgaacaaat aaacctaaat aatatgagtg
    49261 ctaatctttt aggtggcaat gccatcatat caggatttta taatactaaa acggatattc
    49321 ctacggcaca aataaattat aatattagta attttgatgt acaagaaact tataaatttg
    49381 taaatacagc tcaaaaagcc ttgcctttta tgaagtatat aaatggtaaa tataattcaa
    49441 atatgaatat caaaacaaat ttatatcctg atttatctcc gaatcttaat accttaaatg
    49501 gaactgcaag tttcaaaatg ccatgggcaa atattgaagg agcaactatc ataaataaga
    49561 tagccgaaac aacaaaaata aaacaattac aaaaattgcg attagaaaat attgatatca
    49621 aaacaacgtt tgttgatgga aggcttttaa tagaaccatt tactgtaaaa agcaataaaa
    49681 tggatattac aattggtggt tcacaaggat tagatgaaac aatggattat gctattgctt
    49741 tagatgttcc ttgggaggaa ttaaatcaag atgcgactgc atttgcaaaa aatttattgg
    49801 ctaaaaaccc aattccgcaa ttgaataatg caatacctga agttattaga ttaaatttga
    49861 atcttaccgg tacttttaaa aatcctaaaa ttacaatggg aaaacctgat gggaaagtag
    49921 gtggaggaac aatgaaagat gttgtgaaag aacaagctca acaacaaatt cagaacataa
    49981 aagaggatgc catacaagtt aaggaagaat tgaaacaaca aggcaaacag accttagata
    50041 ctttaaaaac acaagtgaaa gaccaagcaa aacaggcttt acaaaatctt ataagtggcg
    50101 ataataatga tagtacaaat acaactaagc ctatcgaaac cataaaaaaa tcaggcgaaa
    50161 gtgttaaaga tgctttgaaa aataaattac cttggatgaa ataatttcta tgtttattta
    50221 gcaataattt gctttttact tttaattaat gtaaatttaa acaaatcctt acattgtgag
    50281 aaagccaaaa aatagaatct taggtggttt actatctatt ctaagaaatg aaattcaaag
    50341 tcatagaact ttagatggtc gcagaaactt cttaaaacag actgcaaagg caactgttgc
    50401 ttttactgtt gcgtcaagta ttccagctgt ttttacttct tgcaaaacgg caactggaaa
    50461 tggtgcttca gtaaatagta ataaaatttt agatattgca atacttggag gaggaatggc
    50521 aggattaaat tgtgccaatc atcttttaga ttctgggtta agttttagaa tttttgaagc
    50581 agacagacgt atcggaggac gtattttgac acattataat gactcattgg gactaggtat
    50641 ttatcctgaa tttggtgctg aatttattga ttcggaccat gaagatatgc ttgctttgtg
    50701 caaagaattt aatttggagt tgttagatat gatagcagag caggaaaaaa ataattggat
    50761 taaagatatt ttttactttg ataatcgaag aattagtgag gctgaaatta taaaagaatt
    50821 taaaaaagtt gcaccaaaaa tagcgaaaga cttagatgct ttgggcgaaa attatgacac
    50881 tgaagaagcc gtaaagtttg ataatatgcc actttcagat tatttaaaat ctttaaaatg
    50941 tgccaaatgg ttaaaggaat tatttgatgc atcttttgtt gccgaatttg gtttagattg
    51001 taaagatcaa tctaccatca atttcttaga tatggtagat acagatactt ctgaaggttt
    51061 taaagtcttt ggaacaagcg atgagcgtta cagagttaaa ggtggtaatt cagaggtaat
    51121 aaagcatttg tcggctaagg tgggaatgga taatatagat attaattatg aagttaatgc
    51181 aattgaagaa ttagaggaca gcacttattt gatatctttt aagaatggag aacaagttaa
    51241 atcgaaatct gtagtatgta caattccata taccatactt cgaaatattg atttgaaatt
    51301 aaaaggaatg tcgccagaaa aaaggaaatg tattgatgaa ttgggctatg gaattaatac
    51361 aaaattactg ttaggttatg atagtttgcc ttggagaagt aaagaaaaca atgcgatggg
    51421 ttatttattt acaactgaga tgaccaatgg ttgggatgca agtgtgaata aaaaagaaga
    51481 taatcctaat ggtgcataca ttgctttctt tggaggagat ttatcacagg aacttgccaa
    51541 taagtctttt aaaaatctct atcaaccacc aacacacgtt tggagaacag gtttacctga
    51601 tgaaacagta gattactatt tgagtcgctt agataagata tttaaacatt caaaagaaca
    51661 ctatattgga agatatgttt ttgtgaattg gattgattat ccatttgtga aagcaagtta
    51721 tagttgttac agaattggac aatggacaag tattgcaggg ttggaaatgc agcctattgg
    51781 aaatttcttg tttgctggtg aacattgcag tgctcaattt caaggattca tgaatggtgc
    51841 tgctgaaact ggcagacgtg tagcagaaga aattttaaag gcaaaagaag ttgttactaa
    51901 taattagtct taaatttggt gggaattcca aaatgtaaga ttaaattatt taactatttg
    51961 caataaaaaa attaaatatg tttgacgaac tagcaaaata taaaaataaa ggtcattttt
    52021 tctttgaaaa aggagatgtt cttaaaacta aaagtaaaga tgtgccgaat ttgccaggtg
    52081 tttattatgt aataaaattg gcaaaaggta aagtggaatt agtttatata gggaaatcgg
    52141 gttcaataca acaaaatggt caatttaaag aacagctttt aaatcaaaga ttgaacaata
    52201 agcaagatgg tatgaatagg gaacaatact ttcttcaaaa aattgagtta gaaaatattg
    52261 atgctttaga tatctattgg tttgttacta tggacgaaca atatcaagac ttacctagct
    52321 ttgttgaagg gttgctatta cagcggtatt ttgaaatcta tgcttgtttg cccaaatgga
    52381 acaaatcctt ctaactcaaa atcaatggag taacgaagat tgagattagt ctaaataaga
    52441 agccgaatgg tggttgatcg ttgtcaaagc ccaaactttt ttagaccata tccataaaaa
    52501 cttaaatttt attattattt ttatggttgt aaccataaaa atatgataga gcgaaataca
    52561 tacattcagc agatagtacc atttatcgac aagccgcaag tcaagattat cactgctatc
    52621 tgaagaagtg gtaaatctta tgtgttgcgt ttattactag aagagcacta ttagtaattt
    52681 tatattttat ttggttttgt tcaataattg gtgtatgttt tgtattttgg aatctattac
    52741 agcaacacct attaatgaaa gacagcgact aatgcttaat aaactttttg acggttttga
    52801 tgggaagttg caaacttcaa aatgggcaaa aattaccaaa acttctaccg acactgcctt
    52861 acgagatata aaagacttaa tagaaaaagg cattttgaaa ccaacagatg aaggagggcg
    52921 aaatgcaaac tataaattat attatgaata gttttatctg actataattt aatttatttc
    52981 attattgaat tatatatact tatttttctt tccttctgaa atggcatctt ctttgttgtt
    53041 tacttgaagt ttgatgtaaa tatttttgat gtgaaatttg acagtatcta aagtaacatc
    53101 aagtaaatta gaaatagatt tataactttt accttctgcc aattgcgaaa gtactttaga
    53161 ttcttgtttg gaaagtggat tgttaattgt ttctttcgca aaagatttta ccaccattct
    53221 tgcaatcttt gcactcattg gagctccacc ttttacgact tcgtctagtg catcaatcaa
    53281 ttctttatag ccattgtttt ttgtaagata gccagaagct cccgcattca ttgcgccaaa
    53341 aacacgttcg ctgttttcaa aaactgtaat aataataatt tcagttttag gcaataatga
    53401 ttttataatt gatgtacctt cgataccatt cattcctggt aaatcaatat ccattagaac
    53461 aatatcgggt tttaacgttt ttatatctac taaggcatct ttacaatttt caaaaacctg
    53521 aattactttg taattttctg ttgcattaat caacaattca aagccagaac caacatctgg
    53581 attatcttca ataattacaa ttttttttaa ttctgatttc attttgtttg ttttaaagtt
    53641 tatagttatg ttgtagtgga agtctaattt taaaaacact tccagtcgtt ttgtcagaat
    53701 tgatatctaa ttttccatta atttttgcaa tacgttctct aatattattt atacctcttt
    53761 gttgaatatt ttcgatatta aaaccatttc cattatctga aaaaacaata tcaatttcgt
    53821 tggtttcttg taacagcaaa agttgtaaat tagtttgtgt ggcgtttgaa tgtttggctg
    53881 tgttcgtcat tatttctttc aaaatataaa tcagttgcct acttacattt gtgtcaaaag
    53941 ttaagttact gtcttgttta atatcagaac tagagtaaaa gctgatgcct aatttttcaa
    54001 aaaaatcttc gccaaaatca cgtgtataaa taaaccattc atcaatataa tcgcttttaa
    54061 aatctattga ccaaataaaa tcttttgttc catgatataa ttctattgat ctttcttcta
    54121 ttgtgtttag taattttttt atttctgctt cactttttcc tatgtccatt ttcaagccaa
    54181 tagattggga aagtatagta atacttgcca gtttattgcc catttcatca tggaaatctt
    54241 ttgctaaatc ttttcttaaa agatttagtt cttcagtttt ttctttaatt ttttcttgta
    54301 attcttcaat ttgttcttgt ttaagggttt ctaatttttg attagttctt tgcaaaactt
    54361 tattaaatga aattaaaaga gaagacatca ataaaataac aaatccaaaa gtgaaaatat
    54421 tatgcgttga tgagttttct ataatataat ttttaaaagc agggttacca taagtatcca
    54481 taaaatatag aatcgtcaag tatattattg atatggctga aataatgctt ccaatagtaa
    54541 tagacctctt gcgaaattca ttttagactt agattataaa ttcctgcaat aa
//
LOCUS       DAOQCW010000004        44800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_273_length_44800_cov_5.233702, whole genome shotgun sequence.
ACCESSION   DAOQCW010000004 DAOQCW010000000
VERSION     DAOQCW010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 44800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44800
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_273_length_44800_cov_5.233702"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1652)
                     /locus_tag="PLS16_00840"
     CDS             complement(<1..1652)
                     /locus_tag="PLS16_00840"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59651.1"
                     /translation="MNDLLNVQMSGRELDDFLSGGDDGVCGMQNIHSGINNVAITSVQ
                     PCMFSDLQESRISCSSDGSTENYGTRWAWKWFWNGPSLTAEHAGQIAFNNSGNPTFIK
                     LCTPGTVSFANIVSPIDPVPHTVGIWENIYWQRQMEGSGGILLPDQWQTLGFVGLTPT
                     DNNLGLGRWHYRRVARFCTGDNGVLDFKEVFSNEIIVEVVPQPVAPVGNKLPNINTVC
                     VGQTITLQNVGYGTPEDAVTNCGIEYQYSVDNGDNWSPISNTLPSIQAVGNVDPTKSI
                     NIIRARVKGNCNYGSCNPSPWMERSWTVDYVTSPSVSGNRDVCKGQSTTLTVTNVIPG
                     ATVKWYLDAALTNEVLPRTQSKSFVVNNNMSIWVLQSRDATGCPSLPTKVDLTVKETT
                     ASLPSSTPVVCLGNSISPSITHTTTGATGIGVATGLPNGVAAFFSGNTITISGVPTQT
                     GTFTYNIPLIGDCPNAKATGIITVVTSNTAGVPSAKPTLCIGTLLPNITHTTLGATGI
                     GTPVGLPTGVTASWSPNTIVISGTPTVSGVFNYTIPLTGGCSG"
     gene            complement(1685..1825)
                     /locus_tag="PLS16_00845"
     CDS             complement(1685..1825)
                     /locus_tag="PLS16_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59652.1"
                     /translation="MNLTQELSSELKIVLLVPGYLKQQQMMQIGAEQIVAVGLEELRL
                     HQ"
     gene            complement(2249..3220)
                     /gene="hemB"
                     /locus_tag="PLS16_00850"
     CDS             complement(2249..3220)
                     /gene="hemB"
                     /locus_tag="PLS16_00850"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNJ59653.1"
                     /translation="MLIRPRRNRVSNAIRNLVQETHLLPQHLVYPLFIIDGQDIKNEI
                     KSLPNVFQLSLDNILLEIKECIDLGIHSFIMFPVVEEKLKDKIASYSYSDDNFYLKAI
                     AVIKKHFPQVCLISDIAMDPYSSDGHDGYVDEFGNINNDATLPILAKMAVAQAKAGFD
                     ILGPSDMMDGRIHFIRTALDENGFSNVGIMSYTAKYASAFYGPFRDALDSAPKHGDKK
                     TYQMNPANSQEALIEADLDVVEGADFLMVKPAVHYLDIIQLLKQNYNLPIVAYHVSGE
                     YAMLHAAAKNTWLDYEKSMQETLLSIKRAGADIIISYAAKDFAKLLI"
     gene            complement(3235..4680)
                     /locus_tag="PLS16_00855"
     CDS             complement(3235..4680)
                     /locus_tag="PLS16_00855"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNJ59654.1"
                     /translation="MRKGIVFFGFILGLGLLFSSCKKENDGKRIAQNKFVEDPSIIQD
                     QYGRQLILHGLNTSASAKSDPLGSPWITEADVDREAIDFGFNFVRYLIFWDGIEPQKG
                     VFDEAYLDRVQERVEWYTKRGMYVMLDMHQDLYAQKFDGDGAPEWAIRSGNNKPIEIG
                     DDMPWWLKNIDPAIIAAWINFWEYKEHKDLQDHYALMWQKAINRFKDNPYVIGYDLMN
                     EPWGGDLIKSFITREFERVQLTAFYNRLIPKIREVDPDGYIFFEPTPAPVTFGFPSTL
                     MPIRDTRVPSRLVYAPHCYPFDTHEGNGYTQSSKKNLIDWENQRKKEVSPIVHGNIPL
                     MTGEFGLGYWQTGFDTYLKDFNNMSDRNQWHWAYWSYDLYGWSPIDGNRNPTPIRDYI
                     VRAYPRAVAGKIVSFDYNPDTRIFNLKYNNNNDIDKPTEIAVPKFCYPNGFDAIVSGA
                     SDYSTSFDEQFQIFKIKSKLNTEITVNIKPK"
     gene            4832..>7673
                     /locus_tag="PLS16_00860"
     CDS             4832..>7673
                     /locus_tag="PLS16_00860"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNJ59655.1"
                     /translation="MKNISFILLFISLKLSLVGASIVQPKLDYIENKGQWHEHVLYKA
                     DLKGGIAFLEKDGITYSFVERIDHHHENKSNIWNFLKNIDNPELKKNEIQKGHAFNFK
                     WVNANPGVNISAKQKQSYFNNYFIGDDENKWASSVGNYRQVVYKNLYYQIDALLYSEQ
                     NSMKTDYLIKVGGNPTDIKLQYNATDGLTINEKGNLKIKTSINEIYELRPYAYQKIGK
                     IRIEVPCEYELTDNILSFKLPKGYDKNYDLIIDPTLIFSTYSGSPVDNWGSSATNDAD
                     GNMFLGGIALGQNYPTTLGAFQRNYGGGDSGSDIGEPSDIVITKFNATGTSRIYSTYL
                     GGNGNELLQSLYCTPQNELVILMTTGSINFPTTTNAFDRTFNGGTSTSIMGIAFPNGT
                     DIALTKLNANGSALVGSTFFGGSENDGQGIYVNYGDETRGDIALTQQGDIIIGTSTAS
                     SNLPGTSGRFQSNFGGAARDGFIAKFNSNLSLLNWASYFGGSANENLNGIKLDKNENI
                     FICGGTNSNNLKNITNNFNGYFDGYIAKINSNATQVIASRYLGTTDYDQAFLIDLDDN
                     DNIVLFGQTAGAYPVSSGVYYNNHAGQFIHKLNNQLNSTIFSTVFGSDSAMNITPTAL
                     MVDICGHIYAVGWGGGFYINENIGNTFGMPVTPDAFQDSTDGNDFYFINLNRDATQLI
                     YATYFGEYGGIGDHVDGGTSRFDKHGVVYQAVCASCAGTDWFPVTPGAYGQHNESNNC
                     NMAGFKFKFDLLSMEITSVDPKTGCINDSVQFSFTATQPAKFYTWEFGDGTSSNLAAP
                     KHKYTQTGTYTIKLKITNEDNCNVVDSTTSTIIISEPSNQTIDTTICQGQSIILGNQT
                     ISTTGKYTENFKNIGGCDSIVTLNVTVKSSSFTTIDTTICQGEFVTINNQKYTNSGIY
                     TIIIPLALGCDSTITLKLKVNPTNNIRTTTYIC"
     assembly_gap    7674..7773
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7774..11556
                     /locus_tag="PLS16_00865"
     CDS             <7774..11556
                     /locus_tag="PLS16_00865"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNJ59656.1"
                     /translation="LKLKVNPTNNIRTTTYICKGETYIFDNQILSEPGIYIAKFKNSR
                     GCDSTITMTLNVIQPDSTSIDTVMCLGHPIIFNNQTISAIGSYTAVLKNIFNCDSIVT
                     LNLTAINLPSKITIDTTICQGDIINFHGQIISTSGTFTASITNSKGCDSIITLNVKTN
                     PLNSVTIDTSICQGGSIIFHNQVIKNSGIYTIQFKNTNGCDSLITLNVSINNLPIVSY
                     KDTLICQGDYILFNNQIINSAGIYSDTIHNTAGCDTVIILNVKINPQSAAIQNETICE
                     GESFVFRNQILTKQGIYYDTLLNHLQCDSIVILYLTVTNQPKFISLDTSICQGDFILF
                     NNQIINSTGIYLDTLYNTIGCDTIIALQLKINPSNNLTINKTICEGDSVNFNNQIIYN
                     AGDYTEKLKNNFGCDSIITLHLQTTNQSFLITIDSTICPGESIVFNQQIIQNNGIYKD
                     TIKSPFLCDTIITLNVHINNTPIQILKDTSICQGEIIIFNNQNIIAEGVYKDTISHLI
                     GCDTIITLKVSIKQHHNITTNQTICLGDSIIFNNQVLYNSGTYTANLTNVFGCDSTVT
                     LNLQTNNTSTTITIDTSICQGQTILFDNIIINKAGNYSYTKQNPFGCDTTFNLNVQVN
                     NTPIQVTLDTTICEGDFIIFNQQIIQTAGNYSSTIHNPFGCDSTINLKVNIDNSSVRV
                     IDTSICAGSTITFNNQTISTAGKYSYQIQNNLGCDSIVTLNLNITEPIFSSLKAQICK
                     GDKYTIGNKEFTQNGNYTITLTSSVTKCDSIVQLELTVVDTLKTTLDIKICEGDSVII
                     SNSVFKNSGTYYIPLKSAAQCDSLVILNLNVIAPTFSTLNEKICDGDSLFVGGQYFKR
                     SGIFTIKTKSSELCDSTITLNLIVNEQKQTNIDTTVCNGTIIYIGNHSYTKTGTFKDT
                     LKTTEGCDSVITLNLNVLPTYNLSLNPEICEGDFYKNGDSTFTKTGNYSIVYTAVNGC
                     DSTVNLQLTVHPKKTTNLEHNICEGDSIVVGTQVFKTTGTYTILLKSSKDCDSTVNLN
                     LQVFQHQERTIDTTICEGTIIKIGNEAFNSTGNYTIHLETIHHCDSTIFLNLLVNPAP
                     IINAVVDSSIIQKGKQVQLNVEATEAYNYVWENDNISNPLIQNPTAIITAPVWFVVEA
                     ENPKTNCKRVDSVFVDVLILPCNKDYIYIPNAFTPNNDGINDIFRVRSNNLLKGTLII
                     FDRWGNKVFESDDINIGWDGFYKGNKQQVETYGYYFSGTCDGGATITLKGSVTILE"
     gene            11631..12275
                     /locus_tag="PLS16_00870"
     CDS             11631..12275
                     /locus_tag="PLS16_00870"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNJ59657.1"
                     /translation="MQDTFRHKGLRRKLVEELKELGIKDEQVLQTIGSIPRHWFIESS
                     FEQKAYENTPFPIGSGQTISNPYTVAYQSELLQLKPTDIVLEIGTGSGYQATVLALLA
                     TKVYSIERHKPLVNHTEKLLQHLNLKNLQCFYGDGFEGLPQFAPFDKIIITAAAPEIP
                     LKLIEQLKPGGLIVLPYGEGEVQQMMRIKKKEDLSIEKELFEYFTFVPMLKGTK"
     gene            12451..13083
                     /locus_tag="PLS16_00875"
     CDS             12451..13083
                     /locus_tag="PLS16_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59658.1"
                     /translation="MKKLKKFALFALVTMLVFQSCKKEETSPLSSPKFKELSANMQKL
                     WSEHMQWTYQTVDAFFHEPDALNANLERLLENQKNIGAAIVPYFGQAAGDTLAKLLTE
                     HIQLAVPVLTAAKENNETALNDALADWNANAKQIGDFLSAANPSNWAKHDMEHMMQMH
                     IDQTVAYSVALLQNDHAKAVELYDEASNHMVLEMSKMLSEGIAKQFPEKF"
     gene            13266..14777
                     /locus_tag="PLS16_00880"
     CDS             13266..14777
                     /locus_tag="PLS16_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59659.1"
                     /translation="MQFVFFTNLLIFIYIPETLAQSDVTKNLNKMSFEEFGVSKDSIP
                     KTFNLPKAEQPGGSTQKELNKIQLERFQHKQRIDSLKKDSLKNISNKLKIDSVNKAIT
                     PKIDTVPTVLKTQTDSLKFQTIDAKEEKEIKISIPKDTTSIKNTEIKNIPNNIVENVI
                     KQDTIPQKATSISQVEKKDTIIKKEEVKIPITKPIENKKADTIQTTKPVSNSVIISKI
                     DTIKKEVIQQPLSKPESDFSTQPITKTSKETIKNEIEDIAIQNKNQLDSIKDKPLKDF
                     YFETQPLINKNGKVVAGESAKQAEIKAPTEIKPNYTAEELAEQLKKEKYNQYLREADS
                     IRFSNQKMLDSLLATLNVKVPVEVKPKDYIEIYVSGGGLTGGLNSKTYDRTIFYPNGL
                     IQREYHSKLGGVVKYEKKISKEELTQLAQFIVDLGFFDFEDEYECAKDDVACKYRMTQ
                     NPSPIPLTITVAVGVRRKNIDIDFFAPRMEQNWVNYPKNIEKILNAIYSLAEQ"
     gene            14867..15754
                     /locus_tag="PLS16_00885"
     CDS             14867..15754
                     /locus_tag="PLS16_00885"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNJ59660.1"
                     /translation="MKTLKTILFSFGLLISVTTIAQDGVNGVNGIYYSFTEALKNPLA
                     VKQLVLNNKGYYVLPDTLLAFPNLEILDLALNNISEIPNWIMYLTKLQSINLNFNNFK
                     SLPLALCYMENLSIIAINRNCINSIPEQIKQLGKLEFLDISNNEFESIPPVIFKIESL
                     KQLIMMDGKLKEIPKEISNLKNLYKVIFSLNQVTEIPDELILLPKIKYIAFANNEIKT
                     INPNICKAKNLNYIAVVGNKLTKFPDCIIDLPNIEYINAGDNFYSKADELYYTQLCYT
                     KGTIPYYTFDPEMDFIKFH"
     gene            15800..16639
                     /locus_tag="PLS16_00890"
     CDS             15800..16639
                     /locus_tag="PLS16_00890"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HNJ59661.1"
                     /translation="MKSTLSSLILLISFLSFAKAQEFTMPVLSPSAKVVQQFSTSSIS
                     IEYSRPSVRNRVIFGDLVPFGKAWRTGANAATKINFGEDVNFGGQNIEAGTYSLHTIP
                     NPTEWTVILSKDLETTNAANYKEEDNIAKINIKATNTKDKIETFTISINDITTISATI
                     QLAWENTIISIPVIADNKAKIFAYLEKELKKTNPIYQKAANFYLEENYKLDDALTYIN
                     KAIEGNPNAYWLFWTKAQILAKLNKKTEAIQAGEITLEKAKLAGSGDEYQKKLDELKK
                     SFK"
     gene            complement(16723..16941)
                     /locus_tag="PLS16_00895"
     CDS             complement(16723..16941)
                     /locus_tag="PLS16_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59662.1"
                     /translation="MGTISVIFGVLSVLGMLLGFIPFLGWFNWLNIPFAVVGLILALV
                     SNSRNGKILNIVAIIIGVIRLFLGGGII"
     gene            17036..18985
                     /locus_tag="PLS16_00900"
     CDS             17036..18985
                     /locus_tag="PLS16_00900"
                     /inference="COORDINATES: protein motif:HMM:NF016915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNJ59663.1"
                     /translation="MKLIYSLIICFITSITIYAQSITEYNLPPAPIFACGDAPSKLNQ
                     ILYGAIPPNSSNKPIVVFVHGWGDNGYGWFVVKNEWYKKCYAEGFRTAFLFQPVAGSY
                     QENGKIIAEMLKKIIKKYNSNNIVIVAHSKGGVDVEWALFNYNMADKVNGVISMSSPY
                     FGTPLADYLGNPIIRSIAELIPIVSDIVKGGGTLQMQPAYSVGVMRQMFDNHPQNNPQ
                     KFRCFGAYNGNHKTELPSPNIPDDFLSLVFYDYKPLCLDLPIGKLGGELVSLPMSIGN
                     ILNLLVMVPKYNQNKAGDIKINDGVAPYYSAIRPGSIEISSTTSPTSNINHIDELFSK
                     YTWEMVRDNVYYFNEHPQYIKTTNPESSSNKRTIEPKETLEIQSKMQLIQNKNIHFTV
                     GEHSIANLILIGNYNNIEYKITNSKDEVISSSNINNDAKTIYQIFNKIDINQLPEGYY
                     TLKSSTPINAILVDDSKAEIELDIKDNQISVQLNNWTDNIDNIKVEVTANRNIDEEGN
                     VIWDKIIPIQLDKDVANNRFISKNFNFEKDGTYNISVYAKSNSTQRFLTTSIYNSNKR
                     EFPITKAIQIFPNPTSGDFTVEWQNALEHNNLVQIFDLQGKTMYQKIIDGNKKYLQLN
                     TKLLGLQNGIYLININGKSEKLLVQ"
     gene            complement(18988..19488)
                     /locus_tag="PLS16_00905"
     CDS             complement(18988..19488)
                     /locus_tag="PLS16_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNJ59664.1"
                     /translation="MHSLAFQYLENDHNQLEAHLNKAIEDFDNIDMEAYDKFRRGLLR
                     HISIEEKIAIPTILQLTGAAVEDAERIRSDHGGIGALLVMSPTKSIIATLRSILDVHN
                     AMEEKEDGFYSRLDEIKGVEADELLARMHASPPVPASKYNDKPGVLDAIKRAVVRSGY
                     EYIEVE"
     gene            complement(19571..21175)
                     /gene="bshC"
                     /locus_tag="PLS16_00910"
     CDS             complement(19571..21175)
                     /gene="bshC"
                     /locus_tag="PLS16_00910"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HNJ59665.1"
                     /translation="MNCSIKNFSFAQTNAFSKLMLDYTSNSKDLQTFIQYPNSIDVIP
                     QIIEDKNKENIDRKVLVEVLENQYQSLEQMKLVAENIRLLENENTFCIVTAHQLNIFG
                     GPLYYIYKIAQTISTCNQLKVKYPEYNFVPIYWMGSEDHDFEEINHTYLYNKKIEWND
                     EQGGISGFYKTNSLQPILEELEILISNQTYGNELMTIFRNAYSQDNLASATRTWLHQV
                     FGNYGLVIIDGNNVVFKQQCIEIIKDDLLNHTAFDLVQDTIVKIEELGYKHQAKPRAI
                     NLFYIEKNKRNRIEFDEQEQKYFVVNSDKSFTKEEILKELEKSPEFFSPNVILRPLFQ
                     QKVLPSIMYIGGGGEVAYWLQLKSVFQNYKVNFPQLIVRNSALLCNNNSMKRLEKFNL
                     SIDDMFQSVDELKKDFIDKDNSISIIQERQSLEQTFAAIQEKIKAIDASLVNYVGAEA
                     QKTFKSMENIEARINKSLKQKNEIHLNQIEKLKNQLFPNNSLQERVENFSSYYAEFGK
                     DFINELINSFDVYQKQFLVIELDKKL"
     gene            complement(21179..22492)
                     /gene="rimO"
                     /locus_tag="PLS16_00915"
     CDS             complement(21179..22492)
                     /gene="rimO"
                     /locus_tag="PLS16_00915"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNJ59666.1"
                     /translation="MKVRTNKKDKVNVITLGCSKNLVDSEVLIGHLKHNQIDVEHQSE
                     KNNANIIVINTCGFIDRAKEESINTILDYADLKEKGKIDKLYVTGCLSHRYKDDLEKE
                     IPAVDAYFGTNEMPYLLKKFKIDYQHELIGERFLTTPKHFAYLKISEGCNRTCSFCAI
                     PLMRGGHISKSIEDVVTEAKALAAKGVREIILIAQELTYYGLDIYKKRALADLLYQLN
                     ELEGIEWIRLHYAYPSKFPIEIIEAIKKCNKVCDYLDIPLQHANNAVLDRMKRQITQE
                     ETRALIHEIRKIHPDIAIRTTMLVGFPGETEAEFEDLCSFVKEMRFERLGVFQYSHED
                     GTAAYELEDDVDTDTKEERASILMQIQQEISMQKNEDKIGKEYRVLIDRKESGYFVGR
                     TEYDSPEVDNEVLINAEKFYCRLGDFTNVVVTDATDFDLYADVIE"
     gene            22601..23398
                     /locus_tag="PLS16_00920"
     CDS             22601..23398
                     /locus_tag="PLS16_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59667.1"
                     /translation="MKNLIIALLLLISVAVNAQTIVGCENYDENTGKTTGEYSSWDIS
                     ANGGYVYLHYSQPKKITKPLTLYVDKKNTSGNFVAYATEYFENDVKSGKNFAVYDFKF
                     TEAGTYKIIALADGQELASKILTIQFKEGEEPNSDSSNDENSDEEVDTYYYEESTVVI
                     ATDVDEDYNPIGEADEFYAKRDGTTPLKIVVSNPERFATELFYIDIYYTDPETEKEVK
                     ETTLETEVNPDWIVSSTDYTFKKRGKYYIDVYNGNDVFINSASIEIK"
     gene            23421..24083
                     /locus_tag="PLS16_00925"
     CDS             23421..24083
                     /locus_tag="PLS16_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNJ59668.1"
                     /translation="MSDSLLSNLIQAEQVAIELFREIELRNLIISGKDEAILNEEIYQ
                     LAKLNFGIEKHWHKRIVRCGQNTLVPYKENPPNLKIQDNDIIFLDFGPIIDNWEADLG
                     RTFVVGNNPKHLKIKNEIEQAWLETKDWVLQQKELKASKLYLFVQEKAKEFGWEFGGS
                     IAGHIIGMFPHEKLDPENFKFYIHPENHLDIFQLNKHWILEMHFIDKEQQFGAFYEQL
                     ML"
     gene            complement(24088..25407)
                     /locus_tag="PLS16_00930"
     CDS             complement(24088..25407)
                     /locus_tag="PLS16_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNJ59669.1"
                     /translation="MGIPNIKGNLLLELYNFSNTPIDFLQQQFDKNGNQFKVNILNRN
                     IICTRNPVWLEHVFIKNKKNYTKDFTMKQIEFILGKGLLTNTGESWLNQRRIIQTAFY
                     KGRLESLAEVMEDIIELHLSKLKAMASGTKFFIDKEMLELTATVALETILGEQMNTEM
                     TEVQKKMAELQEFVVKRIKFPPYILLSYLDGSHQKAKQKLNEIDKVLYKIIEKRKVHQ
                     IESFNLIALLLNAVDADTGEKMTDKQLRDELITFYFAGHETTAFGLSWAFYELVQQPD
                     VVEKIRVEVKSVMQNGKIGSDGLRKLSYTAAVVNEALRLHPPAYIVSREALEEDVIEG
                     EPIKKGDMIVVSLMDMHQHQDYWENPKQFTPDRFLNKDADKETYFNPFGAGPRICIGK
                     HFALMEITLVIAKLFAALDFELVPNQKIDYEALFTLKPKNGIQLIKI"
     gene            complement(25540..26043)
                     /locus_tag="PLS16_00935"
     CDS             complement(25540..26043)
                     /locus_tag="PLS16_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNJ59670.1"
                     /translation="MMSNVFNNKVSEARGVLKPFAIKLTRDITDAEDLVQETVYKAIV
                     NYEKFQTGTNLKAWLYTIMKNIFINDYRKKVSRNVIVDNTPNAYLLNSSITISNNAER
                     AFVMEDINLALQKISAELRVPFLMHYKGYKYKEIADKLNLPLGTVKSRIFFARKDLAQ
                     ILEKDYR"
     gene            complement(26149..27681)
                     /locus_tag="PLS16_00940"
     CDS             complement(26149..27681)
                     /locus_tag="PLS16_00940"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HNJ59671.1"
                     /translation="MIAKLNFKPTLLSYVKYSLILIAFLMSPIVIAQNNDAMYYKKEY
                     YKISYSKTITYKTAKDFRGNNINLQADLNYPGDASDNSGNVKRPAVILYHGGGFFPPG
                     GRNNVINKQFSDYFNKRGFVAIPASYRLGWNYKGKGIQDVLTACFTTNPEDYKDAMYR
                     AFQDSRDLVRYLKDHADEFNIDPNAIFVLGYSAGAILALTHLGDDEEMFNADRAKRLG
                     PMPIDEKSTQVAGVISIAGAHTMPNAQYRNVPTVFFHGTCDFAVPYDGEAKMIACPRF
                     PKTYGPDVISENLKKQNIFYEMYSFCGYDHDFLSKGEPATTKMSKGLQYVVDETTEFI
                     NKVINGNATQSKEVTVTSLPEFPNTAAKGGCAKSKNFEACNGSLQPITITNTNNKPIP
                     NLRNELYPNVGQVVDNNNNSNQQEYPEFPLKFELVEKEVSIYEAIQIFVSTPKSTTLT
                     IEIFNIVGSKISQTQVAVNAGESVINLNSSDWNEGNYVIAISEGGEAKYVDFFTLKDT
                     FF"
     gene            27737..29260
                     /locus_tag="PLS16_00945"
     CDS             27737..29260
                     /locus_tag="PLS16_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HNJ59672.1"
                     /translation="MKSQFFNEEHELFRSSVKLFIDREVMPFADQWEKDEKIPRDLFL
                     KLGEQGFLGINHEEKFGGSKADIFYTVAYLEELAKCGYAGVCAAVSVHQYMATNHLAE
                     AGSDELRECFLRPAIEGKKVGAIAITEPFGGSDVQSMRTTAVLEGDHYVINGSKTFIT
                     NGHFADFVVVACKTDANAGINGISLIVVERGTPGFTSSQLKKIGWHSSDTGELAFDNV
                     KVPKENLVGKVGMGFFYIMESFQIERLVAAVMGVGGGYACLEQTLKYMNEREAFGKQI
                     KKYQVLRHEMVQLYTELEAGKHMTYNAAWLMANGEVPVKECTMAKMYMTELGNKIVDK
                     CLQIFGGYGYMEDFPIARAYRDARVSTIVGGTTQIMREILAKIIIDDIKYKKVYNDEV
                     ATEGKPWGNPETAKDIILSIPLRIKKDKAANYSTIFNFDISGANGGAYSVVINNGDAV
                     VSEGLNDAAVCTISADDETYANVELGKLDPTAAFMAGQIKVDNIAAMMQFSKMFNRI"
     gene            complement(29247..30068)
                     /locus_tag="PLS16_00950"
     CDS             complement(29247..30068)
                     /locus_tag="PLS16_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HNJ59673.1"
                     /translation="MLDKNRIYQIIFNHDTKAGRTFDIVLLWAILFSVLVVIIESVPY
                     FKLNFYNLFRTVELFLTALFTIEYVLRIWSSPDRKKYLLSFWGIIDLLAILPTYLELF
                     FIDSHYLVTIRIFRLLRIFRILKLSNFSRAANVLIVTLINSSYRISVFFFVVIFIVTI
                     LGTLMYIVEGGKGDFDSIPHSIYWAIVTITTVGFGDIVPTTVAGKFLASFVMLLGYAI
                     IAIPTGIVTAEMTMSRSGKISCLKCSLKQSKENKFCSECGTELSSKENLDKTKFD"
     gene            complement(30110..31441)
                     /locus_tag="PLS16_00955"
     CDS             complement(30110..31441)
                     /locus_tag="PLS16_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59674.1"
                     /translation="MKKILALIVCFIMVQNFGFAADPVQLKSENYLLKYTLVKAHTVE
                     TIKAVWKKNGIPKVILPVKNDVDIYEIIYKAPWIDGTWIEASGLCYVPKVKNKVPTMM
                     YGHGTQIEKDRNVSDGDAQQGICLGFATDGYLALYPDYYGIGKSNARHLYQHSWSEAM
                     SFIYMLYAVEELKVKIGSVATNGQLFLTGYSQGGHSSFAAQKYIEALNDPRFQVTATS
                     PMSGAYDMTGEQEKYMFQEYPRPFYLPYLLTSYQMAYKVLDIDNIYDIFKTPFDTLLP
                     DYFEDNRNKTLYALDSLMPKIPADIVKKQYVNDYVNKPNYPFKERLKENSLTDWKPKA
                     PVQLCYCKGDREVNYKNSEVTYENMKALGVKNIKLNNLSDYLDHNTCAIFAVLSTKYF
                     FDRFRDKGENPKMKDVPKFKKMLSNIIKKKEEKKYLSGGADVAYTTEHKTE"
     gene            complement(31514..31807)
                     /locus_tag="PLS16_00960"
     CDS             complement(31514..31807)
                     /locus_tag="PLS16_00960"
                     /inference="COORDINATES: protein motif:HMM:NF013939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNJ59675.1"
                     /translation="METPTKSDLNNLRLLIDDIDNALLQLFKDRMEVSKAVGEFKKAN
                     QLPIFQENRWNELLKDRLLKGEKIQLSETFIKSIFEAIHEESLRLQNHLQENK"
     gene            complement(31915..32697)
                     /locus_tag="PLS16_00965"
     CDS             complement(31915..32697)
                     /locus_tag="PLS16_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNJ59676.1"
                     /translation="MEFSNLLWDVEDGILIITINRADKLNALNGATIEEIKTAFARAK
                     AVSGLKGIILTGAGEKAFVAGADISEFQGKNQEQAKALAQRGHDVFFSIEQMPIPVVA
                     VVNGFALGGGCELSMACHLRIATQNAKFGQPEVNLGIIAGYGGTQRLIQYIGKSKAME
                     LHLTGDMIDANVALNLGLVNYIENNKDAAIAKAKEIINKTASKGPVAIAKVIECINAY
                     YNECENGFDAEVNAFAALFETEDVKEGVAAFLEKRKPDFKGK"
     gene            complement(32700..34070)
                     /locus_tag="PLS16_00970"
     CDS             complement(32700..34070)
                     /locus_tag="PLS16_00970"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNJ59677.1"
                     /translation="MKELLLLSGLGMGTLLLEIFNLRKLILPIVIIGLLANVGLCIYD
                     YNQPQEVYQMLFLDNTALFITAILSFCTACWFIMNLDLQNDEHTLSDHTSLVLFSLVG
                     AFIISSYINLFMLFIGIEILSIPLYVLAGSNKRNIYSNEAAYKYLIIGAFISSFLLLG
                     ITFLYGATGSFDIRQIAIGTLSPKLNAQLYLIGVLLIFFSISFKASIAPFHFWAPDVY
                     SGSPTRVTAFMVTIVKVAALAAFLRFFIMALGIVHQVYANVILTFALLSVVFANLIGT
                     VQQDVKKLMAYSGITHAGFVFLGMAYNSPNTSGVILYYLATYAITGIALFWVIVELSK
                     QGINEIDGYKGLAKKNPLLAFTLILSLLSMAGIPPMAGFFAKYLVLVQVITLGKVWVA
                     IVAIIASLISVYYYFKLIITSVQTAEIEENDKIKLNLLSKITLVVLNLLIIGMGIFAN
                     YIINLI"
     gene            complement(34073..35545)
                     /locus_tag="PLS16_00975"
     CDS             complement(34073..35545)
                     /locus_tag="PLS16_00975"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNJ59678.1"
                     /translation="MATLILIIIPVLFALLVLVANKNMSKYLSVAGASSSLILTLIIL
                     YLFKSSNGSSQLLAWHAEWLPQIGADFYTKVDGLNVVMILLTALVTPFIFLSVNKEKS
                     YYSDKLFYVLMLLMQSAIFGVFIAKNLFLFYIFWELALIPIFFIVLLYGAENRQKITF
                     NFFVYTLMGSLFMLIAIIYLGTKTSASNFNVEQIYFVAKSLSLNEQYFLFACFFLAFA
                     IKIPIFPFHTWQPNTYVVAPTQGTMMLSALMLKMGLFGLIFWLIPIAPLAWQKLSFFL
                     ILLAAISTIYGGLMAFIQKNFKRLLAYSSMAHVGLIAAGIFAFNLASLQGAIIQMLAH
                     SINAVAMFYIYDIIQTRTQIDEIKKLGGIRAKNTLFSVFFIILLMDSIALPLSISFIG
                     EFSLLYGIFQFNYFLAGFAGLSIVLGTTYMVVAYHRMMHGQTSEAAQNFKALNTSEIF
                     ILSALSVLVILFGVFPNIILNLTESDAIHLIEHVKTILNK"
     gene            complement(35567..37483)
                     /gene="nuoL"
                     /locus_tag="PLS16_00980"
     CDS             complement(35567..37483)
                     /gene="nuoL"
                     /locus_tag="PLS16_00980"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNJ59679.1"
                     /translation="MNQLTILIPFLPLISFLIIALLGKKLPKSIISLLAPGSIIVSFV
                     FALIVLIQQIQHQHTVEIQLFSWINIDHFSVPFAFLIDPLSAIFICIITGVGSLIHIY
                     SIGYMHDDEDYARFFSYLNLFVFFMLLLVMGNNLLLTFIGWEGVGLCSYLLIGFWYKN
                     RAYSRAGNKAFIINRIGDLGFLLGTFLVFGVFGTLNYTQIGSNSNAILNNGHLITAIT
                     LLLFVGATGKSAQIPLYTWLPDAMAGPTPVSALIHAATMVTAGIYLIARCNILFSSAE
                     ITMSLIAIIGVATSLLAAIIAVLQNDIKKVLAYSTVSQLGLIVLALGVGAYTAGVFHV
                     LTHAFFKALLFLGAGSVIHGMSGEQDIRKMGGLKSKMPITHITFLIATIAIAGIPPLA
                     GFFSKDQILASTFVSSKILFGLGLFASLLTSFYMFRLYFLTFHGEFRGTEEQEHHLHE
                     SPKSMTFPLIILAVLSVIAGFIGFPHALGDAIGVPNIFDHFLEETVAPLHAEISLNTE
                     LALMGATTVLILITIFIAKVLFIDKKNIPLSDEEPMSLSKEIVYHKFYVDEIYQVFVV
                     KSLYGISAFINKIVEQFALKNIVDGVGKVVVGLGSVLRYSQVGTISLYIVFMVFAIAL
                     FVMYKFYFLIFHQF"
     gene            complement(37503..38399)
                     /locus_tag="PLS16_00985"
     CDS             complement(37503..38399)
                     /locus_tag="PLS16_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNJ59680.1"
                     /translation="MNGTRIIGLVVLLMMLQGCVTKKKYLATETQKDNIQLSLNQTTS
                     DLAKCEKKFGNKVTDYNNLNDELAKAQAKIKELNKEIDYQKNSNVKLLDQLSNLSVIS
                     KEGAESIKKSLEAIDRQNQEINNLNAQIRTKDSLNLALVMNLKRSLDDVNDQEVDVQV
                     QKGVVLISLSDKMLYKSGSYEITATAGTVLGKIAKIINDYKTFDVLIEGNTDNVPIKN
                     NCITDNWDLSAKRATSIARALQLKYGVDPSRIIAGGRSEFYPKVANDTPANRSINRRT
                     EIIIMPKLDEFFKLLEIKPTGN"
     gene            complement(38407..38727)
                     /gene="nuoK"
                     /locus_tag="PLS16_00990"
     CDS             complement(38407..38727)
                     /gene="nuoK"
                     /locus_tag="PLS16_00990"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNJ59681.1"
                     /translation="MAEVITGVPIQWYIYLSILLFTIGSLGVMLRRNAIIIFMCIELM
                     LNAVNLLLVAFSKYLGDSSGQVFVFFIMVVAAAEVAVGLAILMMIYRNNGSVDIDELT
                     KIKY"
     gene            complement(38727..39230)
                     /locus_tag="PLS16_00995"
     CDS             complement(38727..39230)
                     /locus_tag="PLS16_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNJ59682.1"
                     /translation="MPLTTYLFLFLAVVSVLSALMVILTRNAVYSVLYLIVTFFSIAG
                     HYFLMKAEFLGVVHIIVYTGAIMVLFLYIIMMMNLNKDSDERRKNIHAVLIGLSAGIL
                     LLSLVYAVKGAENSMLPSVNNANVGSINVLGKVLFQQYMLPFEVASVLFLSAMVGAVF
                     LSKKKLD"
     gene            complement(39243..39863)
                     /gene="nuoI"
                     /locus_tag="PLS16_01000"
     CDS             complement(39243..39863)
                     /gene="nuoI"
                     /locus_tag="PLS16_01000"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HNJ59683.1"
                     /translation="MATLQLTNRKKIVSKKEMTFSEKLYLPAIAAGMGITLKHFFKKP
                     ATIKYPEQKRPIAPIYRGQHVLKRDENGAERCTACGLCAVACPAEAITMEAEERRPGE
                     EHLYREEKYAKVYEINMLRCIFCGLCEEACPKEAIFLTDRIVPVDVTREDMVYGKDKL
                     VEFIDQPISIMLRQTTDMPEFKKETFHYHNQTWKDEEEIRKSEKKY"
     gene            complement(39869..40897)
                     /gene="nuoH"
                     /locus_tag="PLS16_01005"
     CDS             complement(39869..40897)
                     /gene="nuoH"
                     /locus_tag="PLS16_01005"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNJ59684.1"
                     /translation="MELVILYKFILVVVVFGISLGIAAYSTYAERKVAAFMQDRVGPD
                     RAGPFGLMQPLADGLKMFMKEEIIPNVSNKFLFILGPSIAMLTASMTSVVLPWGGDLQ
                     IGDVKFSLHIADINVAVLYVFAVVSFGIYGIIIGGWASNNKYSLMGALRASSQMISYE
                     LGLGLSLVALVMTTGTMSVKEIVEQQSGGLWNIFYQPLGFLIFFICSMAELNRAPFDL
                     PECETELVGGYHTEYSSMKLGFYLFAEYINMFVTSAIIVSLFFGGYNIPFQKYLPLEG
                     NLLSILQVITFFGKILFSIFVIMWIRWTLPRFRYDQLMKLGWLILIPLALFNIFGTGI
                     VMFLTGKI"
     gene            complement(40897..41925)
                     /locus_tag="PLS16_01010"
     CDS             complement(40897..41925)
                     /locus_tag="PLS16_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNJ59685.1"
                     /translation="MPKVTIDNVTIDVPEGTTILHAARMIDEQHGTNVAPPTMCYYSK
                     LETTGGYCRTCLVKVEKGSEKDPRPMPKPVASCRTTVMDGMVVTNTTSEEIKEARGGV
                     VEFLLINHPLDCPVCDQAGECDLQNLSYEYGKMETRTEFRRRTFEPIDIGDKIKLNMN
                     RCIMCYRCVKVCDQITDERVHGVLHRGYHSEISTYIEKAIDNDFSGNVIDVCPVGALT
                     DRTFRFKSRVWFTKPVDAHRDCKTPKCNGRVRLWLKGADVLRVTGRKDRWGEVEEFIC
                     NECRFDKKATSDWVIEGPTKVNHHSVIAQGQYEFSPLRLHQTKQKGLNGVRAELGEGA
                     LNPNNIKK"
     gene            complement(42159..42407)
                     /locus_tag="PLS16_01015"
     CDS             complement(42159..42407)
                     /locus_tag="PLS16_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59686.1"
                     /translation="MKKLTDLEEQLKQEKTNFNKNTKNGKEQQQSLSKQLDTIGQAKT
                     HTTTDTGRNSSIQKNNESSKSSEREKGGGNIERPKLED"
     gene            complement(42417..>44800)
                     /locus_tag="PLS16_01020"
     CDS             complement(42417..>44800)
                     /locus_tag="PLS16_01020"
                     /inference="COORDINATES: protein motif:HMM:NF037420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59687.1"
                     /translation="DWEEFVSKHKALQGFKSLEDLAFSQTLKNVNDTFNEQLDKLIKG
                     TLPKGHIFQLGNAGAILKASGIEELPIELSADRTKEKSEQPNHPFDLNDLKNLTKAVQ
                     NPIAVFDSTKKDGSKIILTELKDKKGNNYVVALRVSNKGAGRLNVEINDVKSVYPKDH
                     TQGVLDWINSEGNLLRYADKEKALNYISVQSTNLIGNGNTEKGSNTKIQHKLNNAVEK
                     IKNFKNPISDKEVDDVRFSVNKNYIGEALYIINKNAKKLRDLKRDLYNFQKYGDVTSG
                     LAERAGVSEFNFSEEKNSLVVYEENGKAYYDIKDIEDAIKNQEREVSFIENNDNYDED
                     DLDFEKSYLNSLESIKETFENNIEKIKGVYMFDLSNELNRLYEFKGKVISKIGGKAIG
                     VHVFPKGDVRELYKIGDFVFHGEDVSDDYTKEEILKLDKLGEISSDIKIDNIKSLEDA
                     ISEIEKKLGIQFMQYPDGTIYGAVLPDGTMYFNPEHLNANTPIHEHTHLFNQVIQQTN
                     PKLWNRMVEAVKGIGLWNEVKNDPNYKNLKTDSQIADEVYSRLTGNKGEVNWQERIAK
                     ADSKSRLAKVGELIKEYWNNVMDILGIKKMSADDFANMTLDKLFSGKEIKGVKDVLEA
                     GNNLNNQIKSATDNVGTFSSESNDIRFSISDNGGQNLSEAERQEIKEDLKLGIRLGNT
                     VEEITQMYAESNDLDISEKDFKTLLKEVQDEIEAENNKGKQADEQETTTKEKDTTEKD
                     SGKKMKTFAYNRVFKGDFPQELKDALKEHNLEHETVIQDLENNLNSPFHSVLN"
BASE COUNT        15901 a         7607 c         6184 g        15008 t
ORIGIN      
        1 ccagaacaac ctccagtcaa aggaatagta taattaaaga caccgcttac tgtaggtgtt
       61 ccacttatta caatagtatt tggagaccaa gaagctgtaa ctccagtagg taatccaact
      121 ggtgttccta ttccagttgc tcctaatgtg gtatgtgtaa tattagggag taatgttcca
      181 atacataaag ttggttttgc agaagggact ccagcagtat tacttgttac aacagtaata
      241 atacctgttg ctttagcatt agggcaatct ccaattaaag gaatattata tgtaaatgta
      301 cctgtttgag ttggtacacc tgaaatcgta attgtattac cactaaagaa tgcagccaca
      361 ccattaggta aacctgttgc tactccaata cctgttgctc ctgtagttgt atgtgtaatt
      421 gaagggctta tagagttccc taagcaaact acaggtgttg aagatggtaa actagcagtt
      481 gtttctttaa cagttaaatc aacttttgta ggtaaagaag ggcaaccagt tgcatctcta
      541 gattgcaata cccaaatgga catattatta tttactacga atgacttact ctgagttctc
      601 ggtaatactt catttgttaa agcagcatcc aaataccatt ttacagtagc accaggtatt
      661 acattcgtta cagttaaagt tgtactttga cctttacata catctctatt tcctgaaact
      721 gaaggcgaag taacatagtc aacagtccat gaacgttcca tccaaggact aggattacaa
      781 cttccgtaat tacagtttcc tttaactcta gctcttatta tatttatact cttagtagga
      841 tctacatttc ctacagcttg aattgaaggt agcgtgttac taattggact ccaattatct
      901 ccattatcta cagaatattg gtactctata ccacaatttg taactgcatc ttcaggagta
      961 ccataaccta cattttgtaa ggttattgtt tgacccacac acactgtatt tatattaggt
     1021 aatttattac ctacaggtgc tactggttga ggtacaactt ccactataat ttcattagag
     1081 aagacttctt taaaatccaa aacaccatta tctccagtac aaaatcttgc aactcttcta
     1141 taatgccatc tacccaatcc aagattatta tctgtaggtg tcaatccaac aaaacctagg
     1201 gtttgccatt gatcaggtaa taaaattcca ccactacctt ccatctgtct ttgccaatag
     1261 atattctccc atattcctac agtgtgagga actggatcta tagggcttac aatatttgca
     1321 aatgaaacag ttcctggagt acacagctta ataaatgtag gattaccaga attattaaat
     1381 gcaatttgtc ctgcatgttc agcagttaaa gatggaccat tccagaacca tttccaagcc
     1441 caacgtgtgc catagttttc tgttgagcca tctgatgagc aagaaattct tgattcttgt
     1501 agatcagaaa acatacaagg ttgaactgaa gttattgcga cattattaat accagaatga
     1561 atattttgca taccacaaac accatcatct cctccactta aaaagtcatc taattctcta
     1621 cctgacattt gcacattgag taaatcattc ataggaaccg cagtacgagt ttgtggaata
     1681 cctcctactg gtgtaagcgt aattcctcca aaccaactgc cacaatttgt tccgccccaa
     1741 tttgcatcat ttgttgttgt ttgaggtaac caggtaccag aagaactatt tttaactctg
     1801 aactcaattc ttgggtcagg ttcattaaga acaccaccac agtcattcag ttagtacccc
     1861 agcctccatt taacatagac atcgaattga ccgtaacaga taagtcgatc gtaggagcag
     1921 ctccaaagga attgtgttgc aagaaaatta aaacaagcaa caaacttgtt aattttaata
     1981 atctaagttt cattctattt atcatattta caaatattta caaaataatg ttaaaaaatt
     2041 gaccaatatc aagacataat tatcaaaatt tagtcaatat tatgcaaata ttataaaaaa
     2101 gtagataatt aatcaattta tgttaattat attatgcaca aacgaattat acttatccat
     2161 attttatcaa aactcttgtg cataaaccta gatttatgat taaatttcac aataaaaact
     2221 aattgtatat gattgtagta ataagatatt atattaataa ttttgcaaaa tcctttgcag
     2281 cataggagat gataatatct gctccagcac gtttaatact taataaggtt tcttgcattg
     2341 atttctcata atctaaccaa gtgtttttag cggcggcatg taacattgca tattctccac
     2401 tcacatggta agcaacaatt ggtaaattat aattctgttt caatagttga ataatatcta
     2461 aataatgaac tgctggtttt accattaaaa aatcagcacc ttcaacaaca tctaaatcag
     2521 cttctataag tgcttcctga ctatttgcag ggttcatttg gtacgttttt ttatcgccat
     2581 gtttgggtgc tgaatctaaa gcatctctaa aaggtccata aaatgcactt gcatacttgg
     2641 cagtataaga cattattcca acattagaaa aaccattttc atctaatgct gttctaataa
     2701 agtggattct tccatccatc atatcagaag gtccaagaat atcaaaacca gcctttgctt
     2761 gtgctaccgc catttttgct aatattggca aagttgcatc attgtttata tttccaaact
     2821 catctacata accatcatgc ccatcagaac tataaggatc cattgcgata tcacttatta
     2881 gacaaacttg aggaaaatgt tttttaatca ctgcgattgc ttttaaataa aagttatcgt
     2941 cagagtaact ataagaagcg attttatctt ttaacttctc ctctaccaca ggaaacatta
     3001 taaaagaatg aatacctaaa tctatacact ctttaatttc caacaatata ttatccaagc
     3061 ttaattgaaa tacattaggc agagatttta tttcattttt gatatcttgt ccatcaatga
     3121 taaataatgg atacactaag tgttgtggca ataaatgtgt ttcttgtacc aaatttctta
     3181 tcgcatttga tactctgttt cttcttggtc taataagcat aatgtaaaat tacattattt
     3241 tggttttata ttaactgtaa tttcagtatt tagtttagat ttaatcttaa aaatttgaaa
     3301 ttgttcatca aaagaagttg aataatctga agcaccactt actatagcat caaaaccatt
     3361 aggatagcag aatttaggaa ccgcaatttc agtaggctta tcaatatcat tattattgtt
     3421 atactttaaa ttaaaaattc tagtatcagg attataatca aaagacacta tctttccagc
     3481 aacagctctt ggataagcac gaacaatata atctctaatt ggagttggat ttctattgcc
     3541 atcaattgga gaccatccat ataaatcata actccaatat gcccaatgcc attgatttct
     3601 atcagacata ttattaaaat cctttagata ggtatcaaaa ccagtttgcc aatagcctaa
     3661 tccaaattca ccagtcatta atggaatatt tccgtgtact atagggctca cttctttttt
     3721 tctttgattt tcccaatcaa ttaaattttt cttagagctt tgagtataac catttccttc
     3781 atgagtatca aaaggataac aatgtggtgc ataaaccaaa cgacttggaa ctctagtgtc
     3841 tctaataggc attaatgttg aaggaaatcc aaacgtaact ggtgcaggcg ttggctcaaa
     3901 aaagatatat ccatcaggat ctacttctct aattttgggt attaatctat tataaaaagc
     3961 ggttagttgc actcgctcaa actctcgtgt aatgaatgat tttattaaat cacctcccca
     4021 aggttcgttc attaaatcat atcctattac ataaggatta tctttaaaac gattaatcgc
     4081 tttttgccac attaaagcat aatggtcttg caaatctttg tgttctttat actcccaaaa
     4141 atttatccat gctgcaataa ttgctgggtc aatatttttt aaccaccaag gcatatcgtc
     4201 tcctatctct attggcttgt tatttcctga acgaattgcc cattcaggcg caccatcacc
     4261 gtcaaacttt tgtgcatata aatcctgatg catatctaac atgacataca ttcctctttt
     4321 tgtgtaccat tctacacgct cttgtactct atctaagtaa gcctcatcaa atacgccttt
     4381 ttgtggttca attccatccc aaaagattaa atatctaaca aaattaaagc caaaatctat
     4441 agcttctcta tccacatctg cttctgtaat ccaagggctt cctaaaggat cagacttagc
     4501 acttgcagat gtattcaatc catgtaaaat taattgtctg ccatattgat cttgaataat
     4561 tgatgggtct tcaacaaatt tattttgagc aatccttttg ccatcatttt cttttttaca
     4621 agaactaaaa agaagtccta atcctaaaat aaatccaaaa aaaacaattc cttttctcat
     4681 aaaaaattaa tgttgcacaa gatagccatt cttttttacc tacccaaata tagttactat
     4741 tttattatga ctaaatgcta ttaaataatc tctaaaaaaa acaataggaa ctcctaaata
     4801 ttttatactt ttactaatta aagctttagg tatgaaaaat attagtttta ttctgttatt
     4861 tatttcatta aaattaagtt tagtaggggc atctattgtc caacctaaat tagattacat
     4921 tgaaaataaa ggacagtggc atgagcatgt tttgtacaaa gccgatttaa aaggaggaat
     4981 cgcttttttg gaaaaagatg gtattaccta ttcatttgtt gaaagaatag accatcatca
     5041 cgaaaacaaa agtaacattt ggaatttcct aaaaaatata gataatcctg agcttaaaaa
     5101 aaatgaaatt caaaaagggc atgcttttaa ttttaaatgg gttaatgcta atcctggagt
     5161 aaatatttca gcgaaacaaa agcaaagcta ttttaataat tattttattg gagatgatga
     5221 aaataaatgg gctagttcag tggggaatta cagacaagtt gtttataaaa atctatacta
     5281 tcaaattgat gctttactct atagtgaaca aaattctatg aaaactgact atttaattaa
     5341 agttggtgga aatcctacag atataaaact tcaatataat gcaacagatg gattaactat
     5401 taatgaaaaa ggtaatttaa aaataaaaac ctctatcaat gaaatatacg aattaagacc
     5461 ttatgcctat caaaaaatag gaaaaattag aatagaagtt ccttgtgaat atgaattgac
     5521 tgacaatatc ctttcattta aattgccaaa aggttatgac aaaaactatg atttaattat
     5581 tgacccaact ttaatattct ctacatattc aggaagccca gtggacaatt ggggttcttc
     5641 tgcaacgaat gacgctgatg gcaatatgtt tttaggtggt atcgctttag gtcaaaatta
     5701 tccaacaaca ttaggtgctt ttcaaagaaa ttatggtggt ggtgatagcg gcagtgatat
     5761 tggtgagcct agtgatattg tcatcacaaa atttaatgca actggtacaa gtagaattta
     5821 ttcaacttat ttaggtggca atggaaacga acttttacaa agtttatatt gtacaccaca
     5881 aaatgaattg gttattttaa tgactactgg ttctattaat ttcccaacta ctacaaatgc
     5941 gtttgaccga acatttaatg gcggaacatc aacttctata atgggtattg cttttccaaa
     6001 tggaaccgat attgcactaa ctaaacttaa tgctaatgga agtgcattag tcggttcgac
     6061 attttttggt ggtagtgaaa atgatggaca agggatttat gtaaactacg gtgatgaaac
     6121 acgtggggat attgcactta cacaacaagg tgatattatt attggaacaa gtactgcatc
     6181 aagtaattta ccaggaacat caggtagatt ccaatcaaat tttggtggtg ctgctagaga
     6241 tggatttata gctaagttca acagcaatct ttccttatta aattgggcta gttatttcgg
     6301 tggctcagcc aatgaaaatt tgaatgggat aaaattagat aaaaatgaga atatatttat
     6361 ttgtggtggt acaaatagca ataatttaaa aaatataaca aataatttca atggatattt
     6421 tgatggatat attgctaaaa ttaatagcaa tgcaacacaa gttattgcat caagatactt
     6481 gggaacaact gattatgatc aggcattttt aattgattta gatgacaatg acaatattgt
     6541 tttatttgga caaacagcag gtgcataccc agttagctct ggcgtttatt ataacaatca
     6601 tgctgggcaa tttattcata aactaaacaa tcaacttaat tctactattt tctcaactgt
     6661 ctttggcagt gatagtgcca tgaatattac accaactgca ttaatggtcg atatttgtgg
     6721 acatatttat gctgtcggtt ggggtggcgg tttttatatc aatgaaaata ttggcaatac
     6781 ctttggtatg cctgtaacgc cagatgcatt ccaagattca acagatggaa atgattttta
     6841 ttttattaat ctgaatagag atgcaactca gctaatttac gcaacatact ttggtgaata
     6901 cggtggaatt ggcgaccacg tagatggagg aaccagccga tttgacaaac atggcgttgt
     6961 ttaccaagca gtttgtgcta gttgtgctgg gacagattgg tttcctgtaa cacctggagc
     7021 ttatggacaa cacaacgaat cgaataattg caatatggca ggcttcaaat ttaagtttga
     7081 tttattatct atggaaatca caagcgttga cccgaaaact ggttgcatta atgattctgt
     7141 tcaatttagt ttcacagcaa cccaacctgc aaaattttac acttgggaat ttggtgatgg
     7201 tacaagttcc aatttagctg cacctaaaca taaatacact caaacaggaa catatacaat
     7261 caaacttaaa attactaatg aagataattg caacgtagta gattctacaa cttctactat
     7321 aataattagc gaaccaagca accaaacaat tgacacaacg atttgtcaag gtcaatcgat
     7381 tattttaggc aatcaaacta tttcaacaac aggaaaatat acagaaaatt tcaaaaacat
     7441 tggaggttgc gatagtattg ttaccttaaa tgtaaccgta aagtcaagta gttttacaac
     7501 tattgacact acgatttgcc aaggagagtt tgtaacaatc aataatcaaa aatataccaa
     7561 ttcaggtatt tataccatta ttattccact tgctttagga tgtgatagca ctataacttt
     7621 aaaactaaaa gtcaatccaa ccaataatat tagaacaacc acttatatat gcannnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnttaaaac taaaagtcaa tccaaccaat
     7801 aatattagaa caaccactta tatatgcaaa ggagaaactt atatttttga caatcaaatt
     7861 ttatctgaac ctggcatata tattgctaaa tttaaaaact caagaggttg tgatagtaca
     7921 ataacaatga cactaaatgt tattcagcca gactctacta gtattgatac agttatgtgc
     7981 ttaggtcatc ctattatatt taacaatcaa accatatctg caattggttc ctataccgca
     8041 gtattgaaaa atatatttaa ttgtgatagc attgttactc taaaccttac agcgattaat
     8101 ctaccaagta aaattactat tgatacaaca atatgccaag gagatattat taattttcat
     8161 gggcaaatta tttctacttc agggacattc actgcttcaa taacaaatag taaaggatgc
     8221 gatagcatta ttacattaaa tgtaaaaact aatcctttaa attctgttac aattgacact
     8281 agtatttgtc aaggaggttc aattattttt cacaatcaag ttattaaaaa tagtggtata
     8341 tatactatcc aatttaaaaa tacgaatgga tgtgatagcc taataacatt gaatgtatca
     8401 ataaataact tgcctatcgt ttcatacaag gacactttga tctgtcaagg agattatatt
     8461 ttatttaata atcaaatcat taatagtgct ggtatctatt ctgatactat ccataataca
     8521 gcaggttgcg acactgttat tattttaaat gtaaaaataa atccgcaaag tgcagctata
     8581 caaaacgaaa ctatttgtga aggcgaatcg tttgtattca gaaatcagat actaacaaaa
     8641 cagggcattt actatgatac attattgaac catttgcaat gtgatagtat agtaatactt
     8701 tacttgacag taaccaatca acctaaattt attagtttag atacaagtat atgtcagggt
     8761 gattttattc tatttaataa tcaaataatt aatagcactg gtatatattt agacacctta
     8821 tataatacaa taggctgtga tacgattatt gcacttcaat tgaaaataaa cccttctaat
     8881 aaccttacca tcaacaaaac aatttgtgaa ggagattcag taaatttcaa caatcaaatt
     8941 atatataatg ctggtgatta tactgaaaaa ttaaaaaaca attttggttg tgatagtata
     9001 attacacttc atttacaaac gacaaatcag tcatttttaa tcacgattga tagcacaatt
     9061 tgccctggcg aatcaattgt atttaatcaa caaattatcc aaaataatgg aatctataaa
     9121 gacacaataa aaagcccatt cttatgcgat acgataataa ctttaaatgt gcatataaat
     9181 aatacaccaa ttcaaatttt aaaagacaca agtatctgcc aaggtgaaat aattattttc
     9241 aacaatcaaa atattatagc agaaggtgtt tataaagaca ctatttctca tttaataggt
     9301 tgtgatacaa taattacatt aaaagtaagt atcaaacaac atcataatat aacgactaac
     9361 caaacaatat gcttgggcga ttccattatt ttcaacaacc aagttttata taattcagga
     9421 acatatactg ccaacttaac aaatgttttt ggttgcgata gtactgttac attaaactta
     9481 caaaccaata atacttcaac cactattaca attgatacta gtatttgtca aggtcaaacg
     9541 attttatttg acaatataat tattaataaa gccggaaact attcttatac gaaacagaat
     9601 ccatttggtt gtgatacaac attcaattta aatgtacaag ttaataatac acccattcaa
     9661 gtaacactag atactacaat atgtgaagga gattttatta tatttaatca acaaatcatt
     9721 caaacagcag gtaactattc ttctacaatt cataatccat ttgggtgcga tagtactatt
     9781 aacctaaaag taaatattga taattcgagt gttagagtaa tagatacaag tatctgtgca
     9841 ggttcaacta taaccttcaa caaccaaaca atttcaactg ctggaaaata ttcttatcag
     9901 atacaaaata atttaggttg tgatagtatt gttactttaa atttaaacat cacagaacct
     9961 atatttagtt cactaaaagc tcaaatttgc aaaggagata aatacactat tggcaataaa
    10021 gaatttacac aaaatggaaa ctatacaatt acattaacgt catctgtaac taaatgcgac
    10081 agtatcgtac aattggaact cacagtagtt gatacattaa aaactacttt agatattaag
    10141 atttgcgaag gagatagtgt aataattagt aactctgtat ttaaaaacag tgggacttac
    10201 tatatcccat taaaatctgc tgcacaatgt gatagtttag taattttaaa tcttaacgta
    10261 attgctccaa cattttcaac cctaaacgaa aaaatttgtg acggtgattc tttattcgtt
    10321 ggagggcaat attttaaaag aagtggaata tttacaataa aaacaaaatc ttcagagctt
    10381 tgcgatagta caattacttt gaatctaatt gtaaatgaac aaaagcaaac aaatattgac
    10441 actacagttt gtaatggaac tatcatttat attggaaacc attcctatac aaaaactgga
    10501 acatttaaag atactttaaa gactactgaa ggttgtgata gtgtaattac cttaaactta
    10561 aatgttttac caacttataa tttaagcttg aatcctgaaa tttgtgaagg tgatttttat
    10621 aaaaatggag attctacctt tacgaaaaca ggcaattatt ccattgttta tactgcagtg
    10681 aatggttgcg atagtactgt aaatcttcaa ttaacagttc atccaaaaaa gacaactaat
    10741 ttagaacata atatctgcga aggagattct attgtagttg gaacacaagt tttcaaaact
    10801 actggaacat atacaattct tttaaaaagt tcaaaagatt gtgatagtac tgtcaattta
    10861 aacttacaag tttttcaaca tcaagaacga acaatagata ctactatttg tgaaggcaca
    10921 atcattaaaa taggtaatga agctttcaat tcaaccggaa actataccat acatttagaa
    10981 acaatacatc attgtgatag tacaattttc ttaaatttat tagtaaatcc tgcaccaatt
    11041 attaatgccg tggttgattc gagcattata caaaaaggta agcaagttca attaaacgtt
    11101 gaagcgactg aagcttataa ttatgtttgg gaaaatgata atattagtaa tcctctaata
    11161 caaaacccaa cagcgataat aactgcacca gtttggtttg tagtagaagc agagaatcct
    11221 aaaaccaatt gtaaacgtgt agattcagta tttgtagatg ttttaattct tccttgcaac
    11281 aaagattata tctatattcc taatgcattt acaccaaaca atgatggcat caatgatatt
    11341 ttcagagtac gctcgaataa tttattgaaa ggtacattaa taatatttga tagatgggga
    11401 aataaagttt ttgaatcaga tgatatcaac ataggttggg atggttttta taaaggcaac
    11461 aagcaacaag tagaaactta cggttattat ttttcaggca cttgtgatgg tggagcaacc
    11521 ataacattaa aaggcagtgt tactatttta gaatagtatt gacaagtgtt gatattccta
    11581 aataaataat ccaaataaaa aactttcttt tgcagaaaag gaaaagtgtg atgcaagata
    11641 cgtttagaca taaaggactc agaagaaaac tagtagaaga actaaaagag ttaggcatta
    11701 aagatgaaca agtacttcaa acaattggtt ctattccaag acattggttt attgaatctt
    11761 cttttgagca aaaagcttat gaaaatacac cttttccgat aggcagtgga caaacaattt
    11821 ccaatccata tacagtagct tatcaatctg aattattaca attaaagccg acagatatag
    11881 tacttgaaat tggaactggt tctggctatc aagcaactgt attggcttta ttggcaacaa
    11941 aagtatattc tatcgaaagg cataaacctt tggttaatca cactgaaaaa ttacttcagc
    12001 atttgaattt aaaaaaccta caatgctttt acggcgatgg atttgaaggc ttaccacaat
    12061 ttgctccatt tgataaaatc atcataacag cagcagctcc agaaattcct ttaaaattaa
    12121 ttgaacaact aaaaccaggt ggtttaattg ttttacctta tggtgaaggt gaagtccaac
    12181 aaatgatgag aataaaaaag aaagaagatt taagtattga aaaagaactt tttgaatatt
    12241 ttactttcgt accaatgtta aaaggaacta aataaactaa aaaattattt aaaaataaat
    12301 ttaatatttt attgggattc aatgcattcc aaacaattaa gttgcttaat ttcaaattaa
    12361 aataaagagt tttcattttt attgtaacct tttcatttct tatttcctct aataggttat
    12421 aatcaatttt ataactctaa aaaaattaaa atgaaaaagt taaaaaagtt tgccttattc
    12481 gctctagtaa ctatgctagt ttttcaatct tgtaaaaaag aagaaacttc tccacttagc
    12541 agtccaaaat ttaaagaatt aagtgctaat atgcaaaaat tatggtctga acacatgcag
    12601 tggacatatc aaactgttga tgcattcttt catgaaccag atgcattaaa tgctaattta
    12661 gagcgattac tagaaaatca gaaaaacatt ggtgccgcaa ttgtacccta ttttggtcaa
    12721 gcagctggcg acaccttagc taaattatta actgaacata ttcagttagc agttcctgtt
    12781 cttactgccg caaaagaaaa caatgaaact gcattaaatg acgctttagc agattggaat
    12841 gcaaatgcaa aacaaattgg agatttttta agtgctgcaa atccttcaaa ttgggcaaaa
    12901 catgatatgg aacacatgat gcaaatgcat attgaccaaa ctgtagctta ttcagtggca
    12961 ttacttcaaa atgaccatgc taaagctgta gaactatacg atgaagccag taatcacatg
    13021 gttttagaaa tgtcaaaaat gctatcagaa ggaattgcta aacaatttcc tgaaaaattc
    13081 taaaaataga aataagtaca tagaattgta tttgataggc tgtccgaaaa gggcagcctt
    13141 ttttagtaca tcaacttaat attatagcag caagttttca aaataagaaa ataatattca
    13201 aacaattaaa aaagaatgat ttgatttttt ttacatttgt acttatgcaa aatatcctaa
    13261 aaataatgca gttcgttttt tttactaact tattaatttt tatttatatt ccagaaacac
    13321 ttgcccaatc tgatgttact aaaaatttga ataaaatgag ctttgaagag tttggcgtgt
    13381 caaaagatag cattccaaaa acttttaatt tacctaaagc tgaacagcct ggtggatcta
    13441 ctcaaaagga actaaataaa attcaattag aaagattcca acacaaacaa agaatagatt
    13501 ctttaaaaaa agacagtcta aaaaacatct caaataagct aaaaattgat agtgtaaata
    13561 aagcaattac acctaaaata gatacagttc ccacagtatt aaaaactcag actgacagtc
    13621 tgaaatttca aactattgat gctaaagaag aaaaggaaat aaaaattagt attccaaaag
    13681 atactacatc catcaaaaat accgaaataa agaacattcc aaataacatc gttgaaaatg
    13741 taataaaaca agatactatt cctcaaaaag ctacctctat ctcacaagta gaaaaaaaag
    13801 atactataat taaaaaagaa gaagtaaaaa tccctataac taagcccatt gaaaacaaga
    13861 aagcagacac cattcaaaca acaaaacctg tatcaaatag tgtaattatt tccaaaattg
    13921 ataccataaa aaaagaagtc atccaacaac cgctttcaaa acctgaatca gattttagta
    13981 cacaacctat cactaaaact tcaaaagaaa caataaaaaa tgaaatagaa gatatagcca
    14041 ttcaaaataa aaaccaatta gattctatta aagacaaacc actaaaagat ttctattttg
    14101 aaacgcaacc attaattaat aaaaatggaa aagttgttgc aggagaatct gcaaaacaag
    14161 ctgaaataaa agcaccaaca gaaataaaac caaactatac cgcagaagaa ttggctgaac
    14221 aactaaaaaa agaaaaatac aatcaatatt tacgtgaagc agactctatt cgatttagta
    14281 accaaaaaat gctagatagt ttattagcaa ctctaaatgt taaagttcca gtcgaagtta
    14341 aaccaaaaga ttatatagaa atttatgtaa gtggcggtgg tttaactggc ggattgaact
    14401 caaaaactta tgacaggact attttttatc caaatggatt aatacaaaga gaatatcatt
    14461 caaaattagg tggcgttgta aagtacgaaa agaaaatcag caaagaagaa ctcacccaat
    14521 tagcacaatt tattgtagat ttaggcttct ttgattttga agatgaatat gaatgtgcaa
    14581 aagatgatgt tgcttgtaaa tatagaatga ctcaaaatcc atctcctatt ccattaacta
    14641 ttacggttgc agttggtgtt cgtagaaaaa atatagatat agattttttt gctccaagaa
    14701 tggaacagaa ttgggtaaac tatcctaaga atattgaaaa gatacttaat gctatttata
    14761 gtttagcaga gcaataaaat agttgtttaa actagctata atctttaatt ggttgcaata
    14821 gatgctttat tatggtataa attttgtagc ttcataaact caagcaatga aaactttaaa
    14881 aacaatacta tttagtttcg gtttattgat aagcgtaacg acaatcgcac aagatggcgt
    14941 aaatggcgta aatgggattt attattcatt tacagaagca cttaaaaatc cattagctgt
    15001 taaacaactt gttttaaata ataaaggtta ctatgtttta cctgacacat tacttgcatt
    15061 tccaaattta gaaattttag atttagcatt aaataatatc tctgaaattc caaattggat
    15121 aatgtactta accaaattgc aaagcattaa tctaaacttt aataatttca aatcgctacc
    15181 attagctttg tgttatatgg aaaacttgag tattattgca attaaccgaa attgtatcaa
    15241 tagtattcct gagcaaataa agcaacttgg aaaacttgaa tttttagata tttcaaataa
    15301 tgaatttgaa agtattcctc ctgttatctt taaaatagaa agtttaaaac aattaattat
    15361 gatggatgga aaactaaaag aaattccaaa agaaatctca aatcttaaaa atttatataa
    15421 ggtaattttc agtttaaacc aagttactga aattcccgat gaattaattt tacttccaaa
    15481 aataaaatat attgcttttg caaacaatga aattaaaact attaatccaa atatttgcaa
    15541 agctaaaaac ttaaattaca ttgcagttgt tggaaataaa cttacgaaat ttccagattg
    15601 tattatagac ttaccaaata ttgaatacat taatgctggt gataatttct attcaaaagc
    15661 agatgaactt tattacactc aactttgtta tacaaaagga actattccat attatacttt
    15721 tgaccctgaa atggatttta ttaaatttca ttaagaaaga tacccattat attcatcata
    15781 ttaaaaaatt acaactaaca tgaaaagtac tttaagctca ttaattttac taatttcttt
    15841 tttgagtttt gctaaagctc aagaatttac gatgcctgtt ttaagtccaa gtgcaaaagt
    15901 agtacaacaa ttttctactt catcaataag catagaatac agcagaccat cagtaagaaa
    15961 tcgtgtcatt tttggagatt tggtaccatt tggaaaagct tggaggactg gtgcaaatgc
    16021 ggctactaaa attaactttg gagaagacgt aaattttggt ggtcaaaata tagaagctgg
    16081 aacttatagt ttacacacta ttccaaatcc tacagaatgg actgtgattt taagtaaaga
    16141 cttagaaaca acaaatgctg caaactataa agaagaagat aatattgcta aaataaatat
    16201 taaagcaact aacactaaag ataaaataga aacatttaca atcagcatta atgatattac
    16261 aacaatttct gcaaccatac aattagcttg ggaaaacacc attatatcta ttccagtaat
    16321 agctgataac aaagctaaaa tctttgctta tttagaaaaa gagcttaaaa agacaaatcc
    16381 tatttaccaa aaagctgcta atttttattt agaagaaaac tacaaactag atgatgcatt
    16441 aacttacata aataaagcaa ttgaaggcaa tccaaatgca tattggttgt tctggactaa
    16501 agctcaaatt ttagcaaaat taaataaaaa gaccgaggct atccaagctg gagaaattac
    16561 tttagagaaa gctaaacttg ctggaagtgg tgatgaatat caaaagaagc ttgacgaatt
    16621 aaagaaaagt tttaaataat ttttatacta tacaaataaa aaatgggcga cgattttgtc
    16681 gcccattttt tgtatcaata taaagtaaag aaaaactaaa ctctaaataa ttccaccacc
    16741 taagaataat cttattactc caataataat agcaacaata tttaaaattt tcccatttct
    16801 agaattgcta actaaagcaa gaattaaacc aacaactgca aatggaatat tcaaccaatt
    16861 aaaccaacct aaaaacggaa taaatcctaa aagcatgcct aaaacagaaa gcacaccaaa
    16921 aataacactt atagttccca taatttattt ctttaaatac aaatataaag tataatacag
    16981 acaatatcaa ttttgtgtat attttattat atttgataag ccaaacttta gaaacatgaa
    17041 actaatatac agtcttatta tttgcttcat aacttccatt actatttatg cccaaagcat
    17101 tactgaatac aatcttccac cagctccaat atttgcttgt ggagatgctc catcaaaact
    17161 caatcagatt ttatatggag caataccacc aaattcttca aataaaccaa tcgttgtctt
    17221 tgttcatggc tggggcgata atggctatgg ttggtttgtt gtaaaaaatg aatggtataa
    17281 aaagtgctat gctgaaggat ttaggactgc ctttttattt caacctgttg caggttctta
    17341 tcaagaaaat ggaaaaatta tagcagaaat gctaaaaaaa atcattaaaa aatacaattc
    17401 aaacaatatt gtaatagtcg cacacagcaa aggtggtgtt gatgtggaat gggcattatt
    17461 taattacaat atggcagata aagtaaatgg tgttatttct atgtcatctc cttattttgg
    17521 aactcctctt gctgattatt taggaaatcc tataattcgt tctattgctg aactcattcc
    17581 tatagttagt gatattgtaa aaggtggtgg tacgctccaa atgcaaccag cctattccgt
    17641 tggtgtgatg cgtcaaatgt ttgacaacca ccctcaaaat aatcctcaaa aatttagatg
    17701 ttttggtgcc tataatggca atcacaaaac agagctacct tctccaaata ttcctgatga
    17761 ttttttaagc ttggtatttt atgactataa accactttgt ttagatttac ctattggaaa
    17821 acttggtggg gagttggttt cattaccaat gagcattgga aatatattga atctactcgt
    17881 tatggttcca aaatataatc aaaacaaagc tggagatata aaaatcaatg atggtgttgc
    17941 accttattat tctgctataa gacctggttc tattgaaatt tcatcaacaa caagtcccac
    18001 atcaaacatc aatcatatcg atgaattatt ttcaaaatat acttgggaaa tggtaagaga
    18061 taatgtttat tatttcaatg agcacccaca atatataaaa acaaccaacc ctgaatcttc
    18121 atcaaataaa agaactattg aaccaaaaga gactctcgag attcaatcaa aaatgcaatt
    18181 gattcagaat aaaaatatcc attttactgt tggagagcat agtatagcaa atctaatttt
    18241 aattggtaac tacaataata ttgaatacaa aattacaaat agtaaagatg aagttatttc
    18301 tagttcaaac atcaataatg atgcaaaaac tatctatcaa atttttaata aaatagatat
    18361 aaaccaacta cccgaaggtt attatacatt aaaatcaagt actccaataa acgccatttt
    18421 agttgatgat tctaaagccg aaattgaatt agatataaaa gataatcaaa taagtgttca
    18481 actcaataat tggacggaca atattgacaa tattaaagtt gaagtaactg cgaatagaaa
    18541 tattgacgaa gaaggcaatg taatttggga taaaataatt cccatacaac tagataaaga
    18601 tgtcgcaaac aaccgattta ttagtaaaaa tttcaacttt gagaaagatg gtacttataa
    18661 catttcagta tatgcaaaaa gtaattcaac gcagcggttt ttaaccacaa gtatttacaa
    18721 ttctaacaag agagaatttc cgattacaaa ggcaatacaa atttttccaa atccaacaag
    18781 cggagatttt actgttgagt ggcaaaatgc tttagaacac aacaatctag ttcaaatttt
    18841 tgatttacaa gggaaaacta tgtatcaaaa aataatagac ggcaacaaaa aatatttaca
    18901 attaaacacc aaactacttg gtttgcaaaa tggaatttac ctcattaata taaatgggaa
    18961 aagtgaaaaa ctattggtac aataaattta ttcaacttct atatactcat aaccagaccg
    19021 aacaacagct cgttttatag catctaaaac accaggttta tcattgtatt ttgaagctgg
    19081 tacaggtggc gaagcgtgca tccttgctaa taattcatca gcttcaacgc ctttaatttc
    19141 atcaaggcga ctataaaatc catcttcttt ttcttccata gcattatgta catctaaaat
    19201 ggaacgcaat gttgcaataa tagattttgt aggcgacata accaataaag ctccaatgcc
    19261 tccgtggtct gaacgaatac gttctgcatc ttcaacagca gcacccgtaa gttgtaaaat
    19321 agtaggaatg gctatttttt cttctatact aatatgacga agtaatccac gacgaaattt
    19381 atcataagct tccatatcaa tattatcaaa atcttcaatg gctttattta agtgagcttc
    19441 aagttggtta tggtcgtttt ctaaatattg aaaggctaaa ctatgcataa aaacaaatta
    19501 aaactgtgta aaaattaaag tataaagata agttttatat aatgtgctaa aaataaaatc
    19561 caactatatt ttatagcttt ttatccaatt ctatgactaa aaactgtttc tgataaacat
    19621 caaacgaatt aatcaattca ttaataaaat ctttgccaaa ttcagcataa taacttgaaa
    19681 aattttctac tcgttcttgc aaactattat ttggaaaaag ttgatttttt aatttctcaa
    19741 tttgattcaa atgaatttca tttttctgct tcaaactttt attaattcta gcctcaatat
    19801 tttccataga tttaaaagtt ttttgagctt cagcacctac ataatttact aaacttgcat
    19861 caattgcttt tattttctct tgaatcgcag caaatgtttg ttctaaagat tgacgttctt
    19921 gtataataga aattgaatta tctttatcga taaaatcctt ctttaattca tcaacagatt
    19981 gaaacatatc atcgatagat aaattaaatt tttctaatct tttcatgcta ttattgttgc
    20041 ataacaacgc actatttctt acaattaatt gaggaaaatt cactttataa ttttgaaaaa
    20101 cagatttcaa ttgcaaccaa taagcaactt ctccacctcc accgatatac atgatacttg
    20161 gcaacacttt ttgttgaaac aaaggtctta aaattacatt gggtgaaaag aattctgggc
    20221 ttttttccaa ttcctttaaa atctcttctt tggtaaatga tttatcagaa tttactacaa
    20281 aatatttttg ttcttgctca tcaaactcaa tacgatttct tttatttttt tcaatataaa
    20341 ataggtttat tgctctcggt tttgcttgat gtttgtaacc taattcttca atctttacaa
    20401 tcgtatcctg aactaaatca aatgcggtat gatttagcaa atcatctttg ataatttcaa
    20461 tacattgttg cttaaaaaca acattatttc catcaataat aaccaatcca tagtttccaa
    20521 aaacttgatg caaccaagtt ctagttgcac ttgccaaatt atcttgactg tatgcatttc
    20581 taaagatggt catcaattca tttccatacg tttgattgct aattagaatt tctaattctt
    20641 caagaattgg ctgcaaggaa ttggttttat agaaaccaga aatgccacct tgttcatcat
    20701 tccattctat tttcttatta taaagatagg tgtggttaat ttcttcaaaa tcatgatctt
    20761 cgcttcccat ccaataaatt ggtacaaaat tatattcagg atactttact tttaattgat
    20821 tacaagtact aattgtctga gcaattttat agatataata taatggacct ccaaaaatat
    20881 tcaattgatg tgcagttaca atgcaaaaag tattttcatt ttctaataag cgaatattct
    20941 cagcaacaag cttcatttgt tccaaagatt ggtattgatt ttctagtacc tccaccaata
    21001 cctttctatc aatattttct ttgtttttat cttcaataat ttgaggaatt acatcaatag
    21061 aattgggata ctgaatgaat gtttgcaaat cttttgagtt gctagtataa tccaacatca
    21121 attttgaaaa agcattcgtt tgagcaaatg aaaagttttt aatactgcaa ttcatctatt
    21181 attcaattac atcagcatat aaatcaaaat cagtagcatc tgtaactact acattagtaa
    21241 aatctcctaa acggcaatag aatttttcag cattaatcaa cacttcatta tctacttctg
    21301 gcgaatcata ttctgttcta ccaacaaagt agccactctc ctttctatca attaaaactc
    21361 tatattcttt gcctattttg tcttcatttt tttgcataga aatttcttgt tggatttgca
    21421 tcaaaatact tgcacgctcc tcttttgtat ctgtatctac atcatcttcc aattcataag
    21481 ccgcagttcc atcttcgtgc gaatattgaa acacacccaa tcgctcaaaa cgcatttctt
    21541 ttacgaagct gcacaaatct tcaaattctg cttcagtttc accaggaaag cctactaaca
    21601 ttgtagttcg aatggctata tctggatgta tttttcttat ttcatgaatt aatgctcttg
    21661 tttcctcttg cgtaatttgc cttttcattc tatctaaaac agcattattt gcatgttgta
    21721 aaggaatatc taaataatca caaactttat tacatttctt aattgcctct ataatttcaa
    21781 taggaaattt tgaaggataa gcataatgca aacgaatcca ttctatacct tccaattcat
    21841 ttaattgata taataaatct gctaaagcac gttttttata aatatctaaa ccataatagg
    21901 tcaattcttg agcaattaaa ataatttctc ttaccccttt tgctgccaaa gccttagctt
    21961 ctgtaaccac atcttcaata gattttgaaa tatgaccgcc acgcattaat ggaattgcac
    22021 aaaacgaaca agtacgatta cagccttctg aaatttttaa ataagcaaaa tgttttggtg
    22081 ttgttaaaaa acgctcacct attaattcat gctgataatc aattttaaat tttttaagta
    22141 gatacggcat ttcatttgtt ccaaaatagg catcaactgc aggaatttct ttttccaaat
    22201 catctttgta acgatgcgac aagcaacctg taacatacaa tttatcaatc ttcccttttt
    22261 cttttaggtc tgcataatct aatattgtat tgattgattc ctctttggct ctatcaataa
    22321 atccacaagt attaatgacg ataatatttg cattattttt ctcagattga tgttccacat
    22381 caatttgatt atgttttaaa tgtccaatta atacttctga gtccactaag tttttagaac
    22441 aacccagcgt aatgacattt actttatctt ttttattcgt tctaactttc atcttttgca
    22501 aaattaatca aaattggcac gattttaggt taattaatga tataactatt gtataattga
    22561 taaaaattaa acatatttac aaaaataaaa acacaaaatt atgaaaaatt taatcattgc
    22621 tcttctttta cttattagtg tagctgtaaa tgctcaaact attgttggtt gcgaaaacta
    22681 tgacgaaaat acgggcaaaa ctactggcga gtatagctct tgggatatta gtgcaaatgg
    22741 cggttatgtt tatttacatt attctcaacc aaaaaaaata acgaaaccac taactttata
    22801 tgtggacaaa aaaaatacat ctggcaattt tgtggcttat gctactgaat attttgaaaa
    22861 tgatgtaaaa agtggaaaaa actttgcagt ctatgacttt aaatttacag aagctggaac
    22921 ttataagata atcgcattag cagatggaca agagttggct tccaaaattt taactattca
    22981 atttaaggaa ggagaagaac caaattctga ttcctcaaat gatgaaaact ctgatgaaga
    23041 agttgataca tactattacg aagaatctac tgttgttatt gcaacagatg ttgatgaaga
    23101 ttataatccg attggagaag ctgatgaatt ttatgcaaaa agagatggta ctactccatt
    23161 aaaaattgta gtttcaaacc ctgagcgttt tgcaactgaa ttattttata tagacattta
    23221 ctatactgac ccagaaactg aaaaagaagt caaagaaact acattagaaa ctgaagttaa
    23281 tccagattgg attgtttcat ctactgatta tactttcaaa aaaagaggaa aatattatat
    23341 tgatgtttac aatggaaatg atgtctttat caattcagca agtattgaaa tcaaataaga
    23401 tttcaagcat tcttctttaa atgtcggatt ctttattaag caatttaatt caagcagagc
    23461 aagttgctat tgaattattt agagaaatag aacttagaaa tttgattatt tctggtaaag
    23521 atgaagcaat attgaatgaa gagatttacc aattagcaaa gttgaacttt ggaatagaaa
    23581 agcattggca caaaagaatt gttcgttgtg gacaaaacac attagttcct tataaggaga
    23641 atcctcctaa tttaaagatt caagataatg atattatttt cttagatttt ggacctatta
    23701 ttgataattg ggaagctgat ttaggaagaa cttttgttgt aggaaacaat ccaaaacatc
    23761 taaaaattaa aaacgaaatt gaacaagctt ggctcgaaac aaaagattgg gtgttacaac
    23821 aaaaagaatt aaaagcctca aaactttatt tgtttgttca agaaaaagca aaagaatttg
    23881 gttgggaatt tggcggatca attgcaggtc atataattgg tatgtttcct cacgaaaaat
    23941 tagaccctga aaattttaaa ttttatatac accctgaaaa tcatttggat atttttcaac
    24001 tcaacaaaca ttggatttta gaaatgcatt ttatagacaa agaacaacaa tttggagcat
    24061 tttatgaaca attaatgctt tagccaacta aattttaatg agttgaattc catttttggg
    24121 ttttaaagta aacaatgctt cataatcaat tttttggttg ggtaccaatt caaaatctaa
    24181 tgcagcaaat agtttagcaa tcactaatgt aatttccatt aaagcaaaat gtttgccaat
    24241 acaaattctt ggacccgctc caaatggatt aaaataagtt tctttatcag catctttatt
    24301 tagaaatcta tcaggagtaa attgctttgg attttcccaa taatcttgat gttgatgcat
    24361 atccattaag gacacaacta tcatatctcc ctttttaatt ggttctcctt caatcacatc
    24421 ttcttccaat gcttctctgg aaacaatata tgctggtgga tgtaatctta aagcttcatt
    24481 cacaactgct gcggtatagg ataatttgcg aagtccatca cttccaattt ttccattttg
    24541 cattactgat tttacttcta ctctaatttt ttcaacaaca tctggctgtt gcaccaattc
    24601 ataaaatgcc caacttaacc caaatgctgt tgtttcatgt cctgcaaaat aaaaagtaat
    24661 caattcatct cttaattgct tatcagtcat tttttcacca gtatcggcat cgaccgcatt
    24721 taacagaagt gcaattagat taaaactttc tatttgatga acttttctct tttcaattat
    24781 cttatataaa accttatcaa tttcatttag tttttgtttt gctttttgat gtgatccatc
    24841 taaataagac aataaaatat aaggtggaaa ctttattctt tttacaacaa actcttgcaa
    24901 ttccgccatt ttcttttgaa cctcagtcat ctctgtattc atctgttctc caagtatcgt
    24961 ttctagagcc acagttgctg tgagttccaa catttcctta tcaataaaaa acttagttcc
    25021 agaagccatc gcctttagtt tagaaagatg caattcaata atatcttcca tcacttcagc
    25081 caaactctct aatcttcctt tataaaatgc agtttgaata attcttcttt gatttaacca
    25141 cgattctcca gtattcgtaa gcaagccttt gcctagaata aactcaattt gtttcatcgt
    25201 aaaatccttg gtataattct ttttattttt tataaaaaca tgttctaacc aaacaggatt
    25261 tctagtgcaa ataatatttc tatttaaaat atttacttta aattgattgc catttttatc
    25321 aaattgctgc tgtaagaaat ctattggcgt attggaaaaa ttatacaatt ccagcaatag
    25381 atttcctttt atattaggaa ttcccatgtt tatatttaaa aactaaaatt acaagattga
    25441 aacttaaaaa aaatatttat tttatttaat tcagatttca atcatcaaaa gaaatttata
    25501 agcctaatta agagtgctta aaaaaataag atttagaaat tatctataat ctttctccaa
    25561 tatttgtgct aaatctttac gagcaaagaa tatacggctt ttaactgttc ctaaaggcaa
    25621 atttaattta tccgcaattt ctttgtattt ataacctttg taatgcatta aaaatggtac
    25681 tctaagttca gctgaaattt tttgcaatgc aagattaata tcttccatca caaatgctct
    25741 ttctgcatta ttagaaatcg taatagaaga atttaataaa taagcattgg gtgtattatc
    25801 aacaattaca tttctagaaa ctttttttct ataatcattg ataaatatat ttttcattat
    25861 agtatataac cacgccttca aatttgtacc tgtttgaaat ttctcatagt ttacaatcgc
    25921 cttataaact gtttcttgca ctaaatcttc agcatctgta atatctctag taagtttaat
    25981 agcaaatggt ttcaacacgc ctctagcctc gcttacttta ttgttaaata cattggacat
    26041 cataatattc aatttaatac aataaagtta ggcattataa aaatataaag caaatttata
    26101 agctattgct tatttagttt aacactaatt aaggttagcg tttcttattt agaagaaagt
    26161 atctttaagc gtaaagaaat caacatattt agcctctcct ccttcagaaa tcgcaattac
    26221 ataatttcct tcattccaat cactactatt caaattaatg actgactctc ctgcattcac
    26281 agcaacttga gtttgggaaa tcttagaacc aacaatatta aatatctcaa ttgttaaagt
    26341 cgtagatttt ggtgttgaaa caaatatttg tatggcttca taaatagata cttctttttc
    26401 taccaactca aatttaagcg gaaactcagg gtattcttgc tgattagaat tattattatt
    26461 atctacaact tgtccaacat ttggatagag ttcatttctt aaattcggaa ttggtttatt
    26521 attggtattg gtaattgtaa taggttgtaa gcttccatta caagcctcaa aatttttgct
    26581 tttagcacaa ccaccttttg ctgcagtatt aggaaactca ggtaaagatg ttacagttac
    26641 ttctttactt tgagtagcat tcccgttaat aaccttattt ataaactctg tcgtttcatc
    26701 aacaacatat tgcaaacctt tagacatttt agtggttgct ggttctcctt tacttagaaa
    26761 atcatggtca tatccacaaa aactgtacat ttcataaaag atattttgtt tttttaggtt
    26821 ttctgaaatt acgtctggac cataagtttt aggaaaccgt ggacaagcaa tcatttttgc
    26881 ctcaccatca taaggaactg caaaatcaca agttccgtga aagaaaacag taggcacatt
    26941 tctatattga gcatttggca tagtgtgagc accagcaata cttattactc cagcaacttg
    27001 agttgatttt tcatctatag gcataggtcc taaacgcttt gctctatctg cattaaacat
    27061 ttcttcatca tccccaagat gcgttaaagc caaaattgca cctgcactat aacctaaaac
    27121 aaatatagca ttagggtcta tattaaattc atcagcatgg tctttcaaat atctaaccaa
    27181 atctctagaa tcctgaaaag cacgatacat cgcatcttta tagtcttcag gatttgtggt
    27241 aaaacaagca gttaaaacat cttgaatgcc tttcccttta taattccaac ccaatctata
    27301 agaagcagga atagcaacaa aacctctttt attaaaataa tctgaaaact gtttatttat
    27361 cacattattt ctgcctcctg gaggaaaaaa tccaccgcca tgatataata tcacagcagg
    27421 acgttttaca tttccactat tatcacttgc atcacctgga taattcaaat ctgcttgaag
    27481 attaatatta ttacctctaa aatcttttgc agttttatat gtaattgttt tactataaga
    27541 aatcttatag tattcctttt tataatacat cgcatcatta ttttgtgcaa taactatagg
    27601 cgacatcaaa aaggctatca aaattaaaga atacttaaca taagataaaa gcgtaggttt
    27661 gaaattcagt ttagctatca ttattagttt atttttatat ttttattcaa ataacaagcc
    27721 aaaattaata aaaaaaatga aatcacaatt ttttaatgaa gaacatgagt tattcagaag
    27781 ttccgtaaag ctatttatag atagagaagt aatgcctttc gctgaccaat gggaaaaaga
    27841 tgaaaaaata cctagagact tatttctaaa attaggagag caaggttttt taggcatcaa
    27901 ccatgaagaa aaatttggtg gttctaaagc agatattttt tatactgttg cctacttaga
    27961 agaattagca aaatgtggtt atgctggtgt ttgtgcagcc gtttcagttc atcaatatat
    28021 ggcaaccaac cacttggctg aagctggaag tgatgaattg agggagtgtt ttttacgtcc
    28081 tgcaatagaa ggcaaaaaag ttggtgcgat tgctatcact gaaccttttg gtggttcaga
    28141 tgtacaatcc atgcgaacaa ctgcggtctt ggaaggcgac cattatgtaa ttaatggatc
    28201 taaaactttt attaccaacg gtcattttgc agattttgtt gttgtcgctt gtaaaacaga
    28261 tgccaatgct ggaatcaatg gtattagttt aattgtggtg gagcgtggca ctccaggctt
    28321 tacttcttct caattaaaga aaattgggtg gcactcttct gatactggag aactagcttt
    28381 tgataacgta aaagtaccca aagaaaactt agttggaaaa gttggaatgg gctttttcta
    28441 tatcatggag agttttcaaa ttgaaagatt agttgctgcg gtaatgggcg tcggtggtgg
    28501 atatgcttgc ctagaacaaa cattaaaata tatgaacgaa cgtgaagcat tcggtaagca
    28561 aatcaaaaaa taccaagtat tgcgtcatga gatggttcaa ctttatacag aattagaagc
    28621 tggaaagcac atgacttata atgctgcttg gctgatggca aatggcgaag ttcctgtaaa
    28681 agaatgtacc atggcaaaaa tgtatatgac agaattgggg aataaaattg tagataaatg
    28741 tttacaaata ttcggtggtt atggctatat ggaagatttc ccaattgctc gtgcatatcg
    28801 tgatgctcga gttagtacta ttgtaggtgg aactacacaa attatgcgtg agatattggc
    28861 taaaattatc attgacgata ttaaatacaa gaaagtatat aatgatgaag ttgctactga
    28921 agggaaacct tggggtaatc cagaaacagc aaaagatatt attttgtcca ttccattaag
    28981 aattaaaaaa gataaggctg ctaattattc taccatattc aattttgata tttctggtgc
    29041 aaatggtgga gcatattcag tagttattaa taatggagat gcagtcgttt ctgaaggctt
    29101 aaatgatgca gcagtttgta caattagtgc agatgatgaa acctatgcta atgttgaatt
    29161 aggaaaatta gatccgacag ctgcgtttat ggcaggacaa attaaagtag ataatattgc
    29221 agcgatgatg caattctcaa aaatgtttaa tcgaatttag ttttatctaa attctcttta
    29281 gaagataatt ctgttccgca ctcggaacag aatttatttt ctttactttg ttttaatgag
    29341 cattttaggc aacttatttt tccacttcta ctcattgtca tttctgctgt tacaatacct
    29401 gttggaatag caatgatggc ataacctaat agcatcacaa aggaagctaa aaactttcca
    29461 gcaactgttg ttggtacaat atcaccaaag ccaactgttg ttatcgttac gatagcccaa
    29521 taaatagaat gaggaatgct atcaaaatct cctttcccac cttcaacaat atacatcaaa
    29581 gtacccaaaa tggttacaat aaaaataact acaaaaaaga atacacttat tctgtaacta
    29641 ctattaatca gtgttactat taaaacattt gcagcacgac taaaattaga taacttgagt
    29701 attctaaaaa ttctcaataa cctgaaaatt ctaattgtaa ccaaataatg cgagtctata
    29761 aaaaataact ctaaataagt tggaagtata gctaataaat caataatgcc ccaaaaactt
    29821 aataagtatt ttttcctatc tggcgaactc caaattctta gaacatattc aattgtaaat
    29881 aaagcggtta aaaataactc gaccgttcta aataaattat aaaaatttag tttaaaataa
    29941 ggtacgcttt ctataattac gacaagtaca ctaaaaagaa ttgcccacaa tagaacaatg
    30001 tcaaaagtgc gacctgcttt tgtatcatga ttaaaaataa tttgatatat cctattttta
    30061 tctaacatag gacaatatta caaagatttt tttgatttca gaatttaggt tattccgtct
    30121 tatgttcggt agtataagca acatctgccc cacctgataa atattttttc tcttctttct
    30181 ttttgatgat attagaaagc atctttttaa acttaggaac atctttcatt ttagggtttt
    30241 ctcctttatc tctaaatcta tcaaagaaat atttagtaga tagaacagca aaaatagcac
    30301 aagtattatg gtctaaataa tcacttaaat tattcaattt aatattctta acacccaaag
    30361 ccttcatatt ttcataagtt acctctgaat ttttgtaatt tacttctcta tcgcctttac
    30421 aataacacaa ttgaacagga gccttaggtt tccaatctgt taaactattt tcttttaatc
    30481 tttctttgaa tggatagtta ggtttattta cgtaatcatt tacatattgc tttttaacaa
    30541 tatcagcagg aattttaggc attaaactat ctaatgcata taatgtttta tttctattgt
    30601 cttcaaaata atcaggcaat aatgtatcaa aaggagtttt gaaaatatca tatatattat
    30661 caatatctaa tactttataa gccatttgat aagaagtcaa taaataaggc aaataaaatg
    30721 gacgagggta ttcttgaaac atatattttt cttgctcgcc tgtcatatca taagctccag
    30781 acattggaga agttgctgtt acctgaaatc taggatcatt taaggcttct atatactttt
    30841 gtgctgcaaa agaagagtgt ccaccttgag aatagcccgt taaaaacaat tgaccattag
    30901 ttgcaacact accaatcttt acttttaatt cttctactgc atacaacata taaataaaag
    30961 acatcgcctc actccaagaa tgctgatata aatgcctagc attacttttg ccaataccat
    31021 aataatctgg gtataatgct aaataaccgt cagtagcaaa tcctaaacaa ataccttgct
    31081 gtgcatcacc atctgaaaca tttctatctt tttcaatttg cgtaccatga ccatacatca
    31141 ttgtgggtac tttatttttc acttttggaa cataacataa accagaagct tctatccaag
    31201 taccatctat ccacggtgct ttatatataa tttcataaat atctacatcg ttttttacag
    31261 gcaaaatcac tttaggaatt ccattttttt tccaaacggc tttaattgtt tcaactgtat
    31321 gagcttttac taaggtatat ttaagtaaat aattttcaga tttcaattga actgggtcag
    31381 cagcaaaacc aaaattctga accataataa aacaaacaat taatgctaat attttcttca
    31441 tacctatatt ttggtataaa gatatcacta tttacagtga tgtcaaaatt tgaaatgtaa
    31501 aaaaattata aaatcattta ttttcttgta aatgattttg taagcgtagg ctttcttcgt
    31561 gaattgcctc aaaaattgat tttataaaag tctcagacaa ttgtattttt tctcctttaa
    31621 gcaatctgtc cttcaataat tcattccacc gattttcttg aaaaatgggt aattgatttg
    31681 cttttttaaa ttcgcctacg gcttttgaaa cctccattct atctttaaac aattgtaata
    31741 aagcattatc aatatcatca atcaataggc gtaaattgtt taagtcagat ttggttggtg
    31801 tttccattta tcaatataaa tgtttaatag gtaaatataa tacctaatat tttagatacc
    31861 aaaaattagc aaatttttag cagtacaatc gacaacaaaa ataaaaaaga gtaattattt
    31921 tcctttaaag tctggcttgc gtttctctaa gaatgcagca acaccttctt taacatcttc
    31981 tgtttcaaat agagccgcaa aagcattcac ctctgcatca aaaccattct cacattcatt
    32041 ataataagca ttgatgcact caattacttt agcaattgca acaggacctt tagacgctgt
    32101 tttgtttata atttctttag ccttagcaat tgcagcatct ttattatttt ctatataatt
    32161 tactaagcct aaatttaaag ccacattcgc atcaatcata tcaccagtta ggtgcaattc
    32221 cattgcttta gattttccaa tatattgaat caatctttgc gtcccaccat aaccagcaat
    32281 aatgcctaaa tttacttcag gctgtccaaa tttggcattt tgtgtagcta ttcttaaatg
    32341 acaagccata gacaattcac aaccaccgcc taatgcaaaa ccattaacaa cagcaactac
    32401 aggaattggc atctgctcaa tcgaaaagaa aacatcatgc cctctttgtg ccaatgcttt
    32461 tgcttgttct tgattttttc cttgaaactc agaaatatca gcacctgcta caaatgcttt
    32521 ttctccagct ccagttaaaa taataccttt caatccagat acagctttcg ctctagcaaa
    32581 tgctgttttt atttcttcaa ttgtagcacc attgagtgcg ttcaacttat cagctctatt
    32641 aattgtgatg attaaaatac catcttcaac atcccataaa agattagaaa attccatatt
    32701 tagattaaat tgataatata attagcaaaa atacccattc ctataatcaa aaggtttaat
    32761 acgactaatg taattttaga taataggttt aatttaatct tatcgttttc ttcaatttca
    32821 gcagtttgta cgcttgtaat tattaatttg aaataataat ataccgaaat taaagaagca
    32881 ataattgcaa ctattgcaac ccatactttt cctaaagtaa tgacttgtac taaaactaaa
    32941 tatttagcaa aaaaaccagc catcggtgga atacctgcca ttgataataa ggataagatt
    33001 aatgtaaatg ccaataaagg gttctttttt gccaaacctt tataaccatc aatttcatta
    33061 atgccttgtt tacttaattc cacaattacc cagaaaagag caatgccagt aattgcataa
    33121 gtagctaaat aatacaaaat cacaccagat gtatttggcg aattataagc catacctaag
    33181 aaaacgaaac ctgcatgcgt aattccagaa tatgccatca atttttttac atcttgttgt
    33241 actgtaccga ttaggtttgc aaaaactacc gacagcaaag caaaagtcaa aatcacatta
    33301 gcatatactt gatgtacaat acccaaagcc ataataaaga atcttaaaaa tgcagctaaa
    33361 gcagcaactt ttactatcgt taccataaat gcagtaactc ttgttggcga acctgaatat
    33421 acatcaggag cccaaaagtg gaatggtgca atcgaagcct tgaatgatat agaaaagaaa
    33481 attaagagta cgccaattaa gtataattga gcatttaatt tcggacttaa agttcctatt
    33541 gcaatttgac gaatatcgaa agagcctgta gctccataaa ggaaagtaat acccaacaat
    33601 aagaaactac taataaaggc accaattatt aaatatttat aagcggcttc atttgaataa
    33661 atatttcttt tattagaacc agccaaaaca tataaaggaa tcgaaagaat ttcaatccca
    33721 ataaatagca taaacaaatt gatataactc gaaataataa aagctccaac taatgaaaaa
    33781 agaaccaaag aagtgtggtc ggacaatgta tgctcatcat tttgtaagtc taaattcata
    33841 ataaaccaac aagctgtaca gaaagatagt atagcggtaa taaacaatgc tgtattatcc
    33901 aagaacaaca tttgataaac ttcttgtggt tgattgtaat cgtaaatgca aagtccaaca
    33961 ttggctaata atccaataat aacaataggt aaaatcaact ttcggagatt aaaaatctct
    34021 agcaacaatg tgcccatacc taaacccgat aataacaata gttccttcac acttatttat
    34081 ttagtatagt ttttacatgt tctattaaat gaatagcatc actttctgtc agattaagta
    34141 ttatattcgg gaatacacca aataaaatga ccaatacaga caaagcagat aaaataaaaa
    34201 tctcagatgt atttaatgct ttaaagtttt gagcagcctc acttgtttgt ccgtgcatca
    34261 ttctatgata agcaaccacc atataagttg ttcctaaaac aattgacaag ccagcaaatc
    34321 cagctaaaaa gtaattaaat tgaaagatac catacaataa agaaaactct ccaataaatg
    34381 atattgataa aggcaaagca atactgtcca ttaataggat aataaaaaat acggaaaaca
    34441 acgtattctt cgcacgaata cctcctaatt tcttaatctc atcgatttgt gttcttgttt
    34501 gaataatatc atagatataa aacatagcaa ccgcattgat actatgagcc aacatttgaa
    34561 taattgcacc ttgtaagctt gctaaattaa aagcaaaaat accagcagca attaaaccaa
    34621 cgtgagccat tgaagaatag gctaataatc ttttaaaatt cttttgtata aatgccatca
    34681 atccaccata aatagtacta attgcagcta atagaattaa aaagaaagat aatttttgcc
    34741 aagccaatgg tgcaattgga atcaaccaaa aaatcaaacc aaacaatccc attttcaaca
    34801 tcaaagctga taacatcatt gttccttgag ttggagcaac gacataagta ttaggttgcc
    34861 aagtatgaaa tggaaaaatt ggaattttta tggcaaaggc taaaaagaaa caagcaaaca
    34921 aaaaatattg ttcgtttaaa gataaagatt ttgcaacaaa gtaaatttgc tctacattaa
    34981 agtttgaagc acttgttttt gtgcctaaat agattatagc aattaacata aacagacttc
    35041 ccatcaatgt ataaacaaag aaattaaaag ttattttctg tctattttct gctccataca
    35101 ataaaacaat aaagaaaatt ggaattaatg ctaactccca aaatatatag aacaaaaaca
    35161 aattcttagc aataaatact ccaaaaattg cactttgcat taaaagcatt aaaacataaa
    35221 acaacttatc agaatagtaa gatttctctt tattgacgga taaaaatata aaaggggtaa
    35281 ccaatgctgt caataaaatc atcacaacat ttaagccatc aactttagta taaaaatcag
    35341 caccaatttg tggcaaccat tcagcatgcc aagctaataa ttgactactt ccatttgaac
    35401 ttttaaataa gtacaaaata attagtgtca atattaaact tgaactagca ccagctaccg
    35461 acaagtattt ggacatattt ttattggcta ccaaaacaag caatgcaaat aaaaccggta
    35521 ttataataag tatcaaggtt gccatatcta ttttttcagc agtttattag aattgatgga
    35581 agattaaaaa ataaaattta tacattacaa atagtgctat ggcaaaaacc ataaaaacaa
    35641 tatacaaact aattgttcct acttgtgaat atcttaaaac actacctaat ccgacaacga
    35701 cttttcctac tccatcaact atatttttca aagcaaattg ttccactatt ttattaatga
    35761 atgcagaaat accatataaa gatttaacta caaaaacttg ataaatctca tcaacataga
    35821 atttatggta aacaatttcc ttacttaaag acattggttc ttcatctgaa agtgggatat
    35881 ttttcttatc gataaataat acttttgcaa taaaaattgt aattaatatc aaaactgttg
    35941 ttgctcccat caatgccaat tccgtattca aactaatttc agcgtgtaaa ggtgctactg
    36001 tttcctctaa gaaatgatca aaaatattag gcacaccaat tgcatcacct aaagcgtgtg
    36061 gaaacccaat aaatccagca atgacagaca ataccgccaa aataatcaac gggaaagtca
    36121 ttgattttgg cgattcatgt aaatgatgct cttgttcttc tgtacctcta aattctccat
    36181 gaaaagttaa gaaatacaat ctaaacatat aaaaactcgt caataaagag gcaaataaac
    36241 ctaaaccaaa caatatttta ctagatacaa aagtacttgc tagaatctgg tctttagaaa
    36301 agaaacctgc taatggagga attccagcaa tagcaatagt tgcaattaaa aaagtaatat
    36361 gtgtaatagg cattttactt ttcagacctc ccatttttct aatatcttgc tcgccactca
    36421 ttccatgaat aacactacct gcacctaaaa acaataatgc tttaaaaaaa gcatgtgtta
    36481 atacatgaaa aacaccagcg gtataagctc caacacctaa tgctaaaact attaatccta
    36541 attgactaac cgtcgaatat gccaatactt ttttaatatc attttgaagc accgcaatta
    36601 ttgctgctaa taaagatgtt gcaacaccaa ttattgcaat tagactcatt gtaatttcag
    36661 ctgaagaaaa taaaatatta catcttgcaa ttaaatatat tccagcagta accattgttg
    36721 cggcatgaat taaagcagaa actggagttg gtccagccat cgcatcaggc aaccaagtgt
    36781 acaatggaat ctgagctgat tttccagttg caccaacaaa taaaagtaaa gtaatggctg
    36841 taatcaaatg tccattgttt agaatagcat ttgaattaga accaatttga gtatagttta
    36901 aagtaccaaa aacaccaaaa actaaaaaag ttcccaataa aaaacctaaa tcaccaattc
    36961 tattgataat aaaagctttg tttccagctc tactataagc tctattttta taccaaaaac
    37021 caatcaataa ataagaacat aatcctacac cttcccaacc aataaatgtc aataataaat
    37081 tattgcccat caccaataac aacataaaga atacgaataa attcaaatag gaaaagaatc
    37141 ttgcataatc ttcatcatcg tgcatatatc caatggaata gatatgtatc aaacttccca
    37201 cacctgtaat aatacaaata aagattgctg aaagtgggtc aatcaaaaat gcaaatggaa
    37261 cagaaaaatg gtctatatta atccagctaa ataattggat ttcaacagta tgttgatgtt
    37321 gaatttgctg aattaaaact attaaagcaa agacgaaaga aacaattata ctaccaggag
    37381 ctaacaaact aattattgat ttaggtaatt tttttcctaa aagagcaata attagaaaac
    37441 taattaaagg caaaaatggt attaatatcg tcaattgatt cattatctaa ttcttttaac
    37501 ttttagtttc cagtaggctt aatttctaat aatttaaaaa actcatctaa ttttggcata
    37561 atgataattt ctgttcttct atttatagaa cgatttgcag gtgtatcatt agctacttta
    37621 ggataaaatt cagaacgacc accagcaata attctactag gatctactcc atattttaat
    37681 tgcaatgctc tagcaattga tgtagctcgt tttgcactca aatcccaatt atctgtaata
    37741 caattatttt taattggaac attatctgta ttgccttcaa ttaatacatc aaacgtttta
    37801 taatcattaa taattttagc aatttttccg agaacagttc ctgctgttgc agtaatttca
    37861 taacttccag atttgtataa cattttatca gacaaggaaa ttaaaacaac ccctttttga
    37921 acttgtacat caacttcttg gtcgtttaca tcatctaatg aacgttttaa attcattact
    37981 aaagctaaat tcaaagaatc ttttgttcta atttgagcat tcaaattatt aatctcttga
    38041 ttttgtctat ctattgcttc caatgatttt ttaatactct cagctccttc tttactgatt
    38101 accgaaagat ttgacaattg gtctaataat ttgacattac tatttttttg gtaatcaatt
    38161 tctttattta attccttaat tttcgcttga gcttttgcca attcatcatt taagttattg
    38221 taatccgtaa ctttattacc aaattttttc tcacatttag ctaaatcaga agttgtttga
    38281 tttaaagaca attggatatt atccttttgt gtttctgttg ccaaatattt tttctttgtt
    38341 acacaacctt gtaacatcat taataatact accaatccaa taattctcgt tccgttcatt
    38401 tctatattaa tattttatct tagttaattc atcaatatct acacttccat tatttctata
    38461 aatcatcatt aaaattgcta atccaacagc cacttctgcg gcagccacga ccataataaa
    38521 gaatacaaaa acttgtcctg agctatcgcc caagtatttt gaaaatgcca ccaataataa
    38581 attgaccgca ttcaacatca actcaataca cataaaaatg ataattgcat ttcgacgtaa
    38641 catcacgcct aagcttccta ttgtaaacaa taagatgctt aaatagatat accattgtat
    38701 aggaacgcct gttataactt ctgccattag tctaattttt tcttacttaa aaatacagca
    38761 cctaccattg ctgataaaaa taatactgat gctacttcaa aaggcaacat atattgttga
    38821 aatagcactt ttcctaaaac atttatactt ccaacattag cattgtttac tgaaggtaac
    38881 attgaatttt cagcaccttt cacagcataa actaaactta ataacaaaat ccctgctgat
    38941 agtccaatca aaacagcatg tatattttta cgtcgttcat cggaatcttt atttaggttc
    39001 atcatcataa ttatatataa gaataaaacc ataatagctc cagtataaac tataatatgt
    39061 acaactccaa gaaactcagc tttcattaaa aagtaatgtc ctgcaattga gaaaaatgta
    39121 acaattaagt acaaaacact atacactgca tttcttgtaa gaattaccat taatgcacta
    39181 agtacagaaa cgacagctaa aaacaaaaat aaataggtag ttaatggcat agtattattt
    39241 gtctaatatt ttttttctga ttttctgatt tcttcttcat ctttccaagt ttgattatgg
    39301 taatgaaaag tctccttttt aaattctggc atatcagtcg tttgtcgtaa cataatactg
    39361 ataggctgat ctataaattc aactaattta tctttaccat ataccatatc ctctctagta
    39421 acatcaacag ggacaattct atcggttaaa aatatagcct cttttgggca agcctcttca
    39481 cacaatccac aaaaaataca tctcagcata tttatttcat acactttagc atatttttct
    39541 tctctatata gatgttcttc acctggtctt ctttcttcag cctccattgt aatcgcctca
    39601 gccggacaag caactgcaca taaaccacaa gcagtacatc tttcagctcc attctcatct
    39661 ctttttagta cgtgttgtcc tctatatata ggtgctatcg gtcttttttg ctcaggatac
    39721 tttatagtag ctggtttttt aaaaaagtgt tttaaggtaa tacccatacc cgcagcaatt
    39781 gcaggaaggt ataacttttc agagaaagtc atttcctttt tggatactat tttttttcta
    39841 ttggttaatt gtaaagttgc cataatgctt aaatttttcc tgttaaaaac attacaattc
    39901 cggttccgaa tatattaaat aaagctaatg gtattaaaat caaccagcct aatttcatca
    39961 attggtcata tctaaagcgt ggaagcgtcc aacgaatcca cataattaca aaaattgaaa
    40021 ataatatttt tccaaaaaat gtgataactt gtaatatact taataggttg ccctccaatg
    40081 gtaaatactt ttgaaaagga atattataac ctccaaaaaa taaggaaaca ataattgcag
    40141 aggttacaaa catattgata tattctgcaa ataaataaaa acctaatttc attgatgaat
    40201 attctgtatg atatccacct actaattcag tttcacattc aggtaaatcg aaaggagctc
    40261 tatttaattc agccatagaa caaataaaga aaattaaaaa acccaaaggt tgatagaaaa
    40321 tattccataa acctcctgat tgttgttcta caatttcttt aacgctcatt gttcctgtgg
    40381 tcattactaa ggcaactaat gataagccta atcctaattc ataactaatc atttgggagc
    40441 ttgcacgcaa agcacccatc aaagaatatt tattatttga tgcccaacca ccaatgataa
    40501 tgccataaat tccaaaagaa acgacggcaa aaacatataa gacagcaaca tttatatctg
    40561 caatatgcaa tgagaatttt acatctccaa tttgtaaatc tccaccccaa ggcaacacaa
    40621 cactcgtcat acttgccgtg agcattgcaa tacttggccc taaaataaat aaaaatttat
    40681 tggaaacatt tggaataatc tcttctttca taaacatttt caagccatct gccaaaggtt
    40741 gcattaaacc aaaaggaccc gctctatctg gaccaactct gtcttgcata aatgcggcaa
    40801 ctttacgctc tgcataagta gaataagctg caatacctaa tgaaatacca aaaacgacga
    40861 caacaagaat gaatttatat aaaataacta attccattat ttcttaatat tatttggatt
    40921 aagagctcct tcgcccaatt ctgcacgaac accatttaaa cctttctgtt ttgtttggtg
    40981 taatctcaat ggtgaaaatt cgtattgacc ttgagcaatt acagagtgat gatttacttt
    41041 tgttggacct tcaattaccc aatcacttgt cgctttttta tcaaaacgac attcattaca
    41101 aataaactct tctacttctc cccaacggtc ttttctacct gttacacgta atacatcagc
    41161 acctttcaac cataaacgta ctcttccatt acattttggt gttttacaat ctctatgggc
    41221 atcaacaggt ttggtaaacc ataccctgct tttgaaacgg aaagttctgt ctgttaatgc
    41281 accaacagga caaacatcta tcacatttcc agagaagtca ttatcaattg ctttttcaat
    41341 atacgtagat atttctgaat gatatccacg atgtaaaacg ccgtgtactc tttcatctgt
    41401 aatctgatca caaaccttaa cgcatctgta acacataatg caacgattca tgtttagttt
    41461 tatcttatca ccaatatcaa taggctcaaa agtacgtctt cggaactctg ttctagtttc
    41521 catttttccg tactcataac ttaaattctg taaatcgcac tctccagctt ggtcacaaac
    41581 tggacaatct aaagggtggt taattaataa aaactctacc actccacctc tcgcttcctt
    41641 aatttcttca gaggtagtat tggtaacgac cataccgtcc ataactgttg tcctgcaact
    41701 tgcaacaggc tttggcattg ggcgagggtc tttttcagaa cctttttcta ctttcaccaa
    41761 acaagttcta caatatcctc cagttgtttc taacttagag taataacaca ttgtaggtgg
    41821 tgcaacatta gttccgtgtt gttcatctat cattcttgct gcatgcaaga tggtagttcc
    41881 ttcgggaaca tctatcgtaa cattatcaat agtaacctta ggcataatta ggacgcaaat
    41941 ttacaaagta taaatgacat tattaagaaa taacgttaat tcttttataa ggttttaaat
    42001 tttggacaaa aattggacaa ttgtatcttt ttttagaagt aatttttact ctacaataaa
    42061 tttacataat tagattaaaa taaaatatat gtttttagtg gtactaaaaa gaagatatta
    42121 aaatttagaa tgatagattt acttaggagt acctttaatt aatcttctag tttaggtcgt
    42181 tcaatatttc cgcctccctt ttctctctcg ctgcttttac tgctttcatt atttttttga
    42241 atactgctat ttcttccggt gtctgtggtg gtatgcgttt tggcttgtcc gatggtgtca
    42301 agttgtttgc ttaatgattg ttgttgttct tttccatttt tggtattttt attaaagtta
    42361 gttttttctt gtttaagttg ctcctctaag tctgttaatt ttttcatttc ctctgcttaa
    42421 tttaatacag aatggaaagg agaattgagg ttattttcta aatcctgaat tactgtttcg
    42481 tgttcaagat tatgttcttt caatgcatct tttaactcct gtggaaaatc gcccttaaat
    42541 actctattat atgcgaatgt tttcattttc ttaccactgt ctttttcggt tgtgtctttc
    42601 tctttagtag tagtttcttg ctcatctgct tgttttcctt tgttgttttc cgcttctatt
    42661 tcgtcttgta cttcttttaa aagggtttta aaatcctttt cagatatatc taagtcattt
    42721 gattcagcat acatttgtgt aatctcttct actgtatttc ctaaccttat acccaacttt
    42781 aagtcctctt ttatttcttg cctttctgct tcggataaat tttgtcctcc attgtcggat
    42841 atagaaaacc ttatgtcatt actttcacta ctgaatgttc ctacattgtc tgtggcagat
    42901 ttgatttggt tgtttaaatt atttccagct tccaatacat cttttactcc cttaatctct
    42961 ttaccgctaa ataacttatc taaggtcata tttgcaaagt catcggcact cattttttta
    43021 atacctaaaa tgtccattac attgttccag tattccttta tcaattcgcc taccttagca
    43081 agtctgctct tgctgtctgc ttttgcaatc ctttcttgcc agtttacctc ccctttgttt
    43141 cctgtcaatc tactgtaaac ctcatctgca atttgactat cggtttttaa gttcttatag
    43201 ttagggtcgt tctttacctc attccataat cctatgcctt tgaccgcttc taccattcta
    43261 ttccataact tagggttagt ctgttggata acttgattga ataagtgtgt atgctcgtga
    43321 ataggcgtat tagcgtttaa gtgttcggga ttaaaataca tcgttccgtc aggcagtacc
    43381 gccccataga ttgtgccgtc aggatattgc atgaattgga tacctaactt tttttcaatt
    43441 tctgatatag cgtcctctaa cgatttgata ttgtctattt ttatatcact actgatttct
    43501 cctaatttgt cgagtttcaa tatttcctcc ttagtgtaat catcagaaac atcttcgcca
    43561 tgaaatacaa aatctccaat tttgtacaac tctcttacat ctcctttagg gaatacatgg
    43621 acaccaatcg ctttccctcc tattttagaa ataactttcc ctttaaattc atatagtcta
    43681 tttaattcgt tacttaaatc aaacatatat actcctttaa tcttttctat attgttttca
    43741 aaagtctctt ttatactttc aagactattc aggtatgact tttcaaagtc taagtcatct
    43801 tcatcataat tatcgttatt ttctatgaaa gatacctctc tctcttggtt ttttattgcg
    43861 tcttctatat cctttatatc ataataagcc ttaccatttt cttcataaac aactaaacta
    43921 tttttttctt ctgaaaaatt aaattctgaa actcccgctc tttcggctaa tccacttgtt
    43981 acatccccat acttttgaaa gttgtataaa tcccttttta aatctcttaa tttctttgcg
    44041 tttttattta taatataaag agcttcccct atatagtttt tattaacact aaacctcaca
    44101 tcatcaactt ctttatccga tattgggttt ttaaagtttt tgattttttc taccgcatta
    44161 ttgagtttat gttgtatctt tgtatttgag cctttttctg tatttccgtt tccaatcaaa
    44221 ttggtcgatt gaaccgaaat atagttaagg gctttttctt tatcagcata tcgcaataaa
    44281 ttaccctcac tattaatcca atctaaaaca ccttgcgtgt ggtcttttgg ataaacgctc
    44341 ttaacatcgt ttatttcaac attcaacctc cctgctcctt tgttagaaac ccttaacgct
    44401 actacataat tatttccttt tttatctttt aattcagtaa gaataatctt tgagccgtct
    44461 ttttttgtgc tatcgaatac tgctatcgga ttttgtactg cttttgttag gttttttaaa
    44521 tcgtttaaat caaatgggtg gttgggttgc tccgacttct ctttggttct atcagcactt
    44581 aactcaatcg gcaattcttc aatcccactt gctttcaata tcgctcctgc attccctaat
    44641 tggaaaatat gtcctttggg taatgtgcct tttatcagct tatccaactg ctcattaaag
    44701 gtatcattta catttttcag cgtttggcta aatgccaaat cttccaacga tttaaagccc
    44761 tgtaatgctt tgtgcttgct tacaaactcc tcccaatcaa
//
LOCUS       DAOQCW010000005        41915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_305_length_41915_cov_5.677568, whole genome shotgun sequence.
ACCESSION   DAOQCW010000005 DAOQCW010000000
VERSION     DAOQCW010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 41915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41915
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_305_length_41915_cov_5.677568"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PLS16_01025"
     CDS             <1..470
                     /locus_tag="PLS16_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HNJ59688.1"
                     /translation="GPLIDKYAQSGDVLKFPFSEPKIPELNFSFSGFKTQVLYFLQKQ
                     EKLNPEFVKENMADICASIQYNLIKILMSKLKMASKQFGINEIAIAGGVAANSELRKQ
                     LKSTAQQLDWKVYIPDFQYCTDNAGMIAITAYYKFLKKQFCAINIPIQPRLNF"
     gene            472..897
                     /locus_tag="PLS16_01030"
     CDS             472..897
                     /locus_tag="PLS16_01030"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNJ59689.1"
                     /translation="MTRIDITVPDKILFETHFFIKEEDINFANHMGNERILVFANTVR
                     TNFYKHLDLLEGDWANGHGTIVANHSVKYITEGFLDDEIICKVGVNELTTCSFDMVMQ
                     FIKKETQKTLALVRSAVVYFDYNERRIRELPQSYLDKFL"
     gene            complement(913..3888)
                     /locus_tag="PLS16_01035"
     CDS             complement(913..3888)
                     /locus_tag="PLS16_01035"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HNJ59690.1"
                     /translation="MSSKEQAYNKIARLVERFDEQKEFYKKADYNETQTRRDFIDPFW
                     KALGWDIDNENGYAESYREVIHEDRIKVGSATKAPDYSFRLVGGKRLFFLEAKKPSVS
                     VKDDIAPAYQVRRYGWSAKMPISVISDFEEFAIYDCANKPKPTDKASNGRIKYLQYTE
                     YLNEFDFIWDTFSKEKVLKGSFDKYISSDKNKKGTSTVDKEFLQSLDKWRVDLAVNIA
                     LRNKDISEEELNFIVQHTIDRIIFLRIAEDRSAEPYGGLATDIKSGTFYQNLLYRFYQ
                     ADQKYNSGLFDFQKDKISEKITIDNKVIKEIISELYYPICPYEFSVLSVEILGSAYEQ
                     FLGKQITLSKNGKAVIEEKPEVRKAGGVYYTPQYIVDYIVNNTIGKLTSPNPSKGGEN
                     ITPKEVSRIKIVDPACGSGSFLLGAYQFLLNWHKDFYTQNGKIYKGNKDNPLTPTGEL
                     TTAEKKRILLNNIYGVDLDSNAVEVTKLSLLLKCMEGETKESIEAQAKLFHDRILPTL
                     DNNIKSGNSLIDLDYFDNEIDFGEEKKVKPFSWEKAFPEVFNRNVPIDERLPYKKQFQ
                     KVKKLSDDTDKLLSQLLNEPTKEYKKNNGGFDVIIGNPPYVKVADKHIFNYFNKKFIH
                     QDYQQDLYLLFLEQYKNLLVEGGKLGVIIPNTWLQSIKFRNIRNYLMNYYYWERILHN
                     KDKVFNAVVDTHVLIFEKNNYIKNTELEIDILKHKTITQFQSIDQNNLPENGDIINIL
                     ANEEEKYLFEKIKSKSIQISEIAKSYSGITLYEKGKGNPAQTAETMKVKPFESNDFQK
                     PKGKNWLPLMRGSLMNRYINIWNNNYWVDYGEWLAAPRNRKIWEANEKIIVRQTGDRI
                     IATIIGKDIICRKNLHIIISNKLDHKFILGILNSKLTDFYYFQINPERGEALAEVKKQ
                     HVEQLPIPKNISGKQENEIIKLVEQILQLNKEKQTATLSHQKEQLHHRIEYAEDKINQ
                     IIYALYDLTADEIKIIEDK"
     gene            complement(3972..4059)
                     /locus_tag="PLS16_01040"
     tRNA            complement(3972..4059)
                     /locus_tag="PLS16_01040"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4023..4025),aa:Ser,seq:gga)
     gene            complement(4117..5265)
                     /locus_tag="PLS16_01045"
     CDS             complement(4117..5265)
                     /locus_tag="PLS16_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNJ59691.1"
                     /translation="MNFQLTEEHLSIREAARKFTQDVLKKEVIERDRDMRHPTEELKQ
                     MSEMGFMGMMVDPKYGGTGLDTISYVLAMEEISKLDSSCSVAMSVNNSLVCWGLEKYG
                     TEEQKQKYLVPLAKGEIWGAFALSEPEAGSDATSQRTIAEDKGDYYLLNGTKNWITNG
                     GKASTYLVIAQTHPEKGHKGINVLIVEKGMPGFEIGAKEDKLGIRASDTHSLLFNDVK
                     VPKENRIGDDGFGFTFAMKTLEGGRIGIAAQALGIAAGAYELALEYSKTRKAFGTEIS
                     NHQAIAFKLADMATEIEAARMLVYKAAWLKDSGQDYAVASAMAKYYASEVAMKTTIEA
                     VQIHGGYGYVREYHVERLMRDAKITQIYEGTTEVQKIVISRAVLTGKA"
     gene            complement(5325..6380)
                     /locus_tag="PLS16_01050"
     CDS             complement(5325..6380)
                     /locus_tag="PLS16_01050"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNJ59692.1"
                     /translation="MRNSLDKAPVGVIGAGSFGTAVANLLAENGRVILYSNRSEAVDK
                     MKAERSNIGQQLHENIIPTNNIQDVTNNCYLIFPALSSDKFRTTIQGFAPHLKPDHII
                     IHCTKGFDIHKEDGTSLVDKDLKISLTDVKTMSQVILEETCVKRVGCMSGPNLAREIA
                     NLQPAATVIASKFDEVIREGESAIRSKRLQVYGNNDIYSVELAGVLKNSMALAAGALG
                     GLGFGQNAMAFLITRGIGEIIRLATAMGGNKNAFLGLAGIGDLVATCTSPLSRNYTVG
                     ARLAKGETIEHIIATSSEVAEGIKTVNISKKISESKNIRTPIIDNIYKVIYEDLSVEK
                     ALQYLMEYRWGQDADYV"
     gene            complement(6361..8037)
                     /locus_tag="PLS16_01055"
     CDS             complement(6361..8037)
                     /locus_tag="PLS16_01055"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNJ59693.1"
                     /translation="MIYKPVIDRIEDWPINKISLQKDRILQETISEAILQLKKDYPND
                     QKIREQVQKTLYLERIRIKTEPWEVDPEDEAEFWNGIKKKVLHSVTNETQDSELVAKR
                     NNETLEAIVSRYAHEMMGNFSPKLFWFARQLLRRIFARLFNAHFSSFKELFRPQDALK
                     DKLIVSGAVEKIRALATKGTVVLVPTHFSNLDSAMIGFAMDYIGMPAFQYGAGLNLFN
                     SKFFALFMGNLGAYKLDRRKKNPIYLETLKQFSKTNVMAGAHTIFFPGGTRSRNGAIE
                     TNLKLGLLGTVIDAQREHYENNPVDLAPKIYVVPLVLSYNFVLEASSLVSDYLRRTGK
                     ENYLVKEPAQVPFKAIWNFLWETFSKSSDVHVSLGEPMDVFGNNIDDDGNSLDHNNNI
                     LEIDKYFYTNGIFKRDNQREMIYTQFLGERIIDSFHKNNVVKTAHVVPFVAFELLKKK
                     IGTNDIYTILRLPEEDREIDWEDFKMNMKLILDELYHLNNKGKLKLSQNLITKELDEV
                     IELGIQNISIYHTNLPLLKNKKGNITSEDLQLLYYYHNRMEGYGLEKFIR"
     gene            8137..8916
                     /gene="rsmA"
                     /locus_tag="PLS16_01060"
     CDS             8137..8916
                     /gene="rsmA"
                     /locus_tag="PLS16_01060"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNJ59694.1"
                     /translation="MNVKAQKSYGQHFLKDTQVLHAIVDAVNQSRQVLPLIEIGPGMG
                     ALTQYLIQIEAPLTCIEVDNRCVTYLEENFLNKSKQFNLLNADFLKVDLNKLIAEEAF
                     VVGNFPYNISTQIIFKLLDVYQKVPVVIGMFQKEVAERLAAKHGNKVYGITSILTQLI
                     YDVEILFDIAPESFLPPPKVMSSVIKLTRKKDIPTDFNYSFFKSIVKTSFNQRRKMMR
                     SSLKSVVNKEFLQDKYFDLRPEQLSVADFIELAKKIENYGK"
     gene            8906..9829
                     /locus_tag="PLS16_01065"
     CDS             8906..9829
                     /locus_tag="PLS16_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNJ59695.1"
                     /translation="MENKKAFITADIHPFLQQQLEQKYIVDVDVAVSREKLLSVIEHY
                     EILVITTYLKVDKELIDKALSLKVVARVGSGLENVDVDYAKSKGINVINSPEGNANAV
                     GEHSLGMLLNLMNKINISNLALKQQIWDRESFRGEELDGKTIGIIGYGHTGPAFAKKL
                     AGFEVNILVHDIVSKEQVKQVDIETIQKECDIISLHVPYTSLTHHLINLEFIRKCDKK
                     PIIINTSRGSVVDTPAIIHALKHQNIRGFCVDVFEDEPIGAGKVYPPMLYHQLLNFEH
                     VVATSHVAGWTIESKFKLVEVLWQKMQDFIA"
     gene            9872..10303
                     /locus_tag="PLS16_01070"
     CDS             9872..10303
                     /locus_tag="PLS16_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59696.1"
                     /translation="MAENNQANHAQGMLTGIMITLFAALIGVLAYGSVFTLAHYMGKD
                     FGQLQNTVFTVLSVIVAVVLAQLLSRGIQGNRLSFMQAFLSGWMASLILGMFINFFFT
                     IFIQITKMPPLQEGSFARMMMLFSMLGIFTSLILSLITKKN"
     gene            10305..11252
                     /locus_tag="PLS16_01075"
     CDS             10305..11252
                     /locus_tag="PLS16_01075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNJ59697.1"
                     /translation="MDISVVIPLLNEAESLPELFSWIEKVMQENNFTYEIIAVDDGST
                     DNSWEIIQNLSKENQNIKGIRFQRNYGKSAALQTAFQATNGNVVITMDADLQDSPDEI
                     PELYSMIVREKYHLVSGWKQERFDNKLTKNLPSKLFNFVTQQVSGIKNLHDFNCGLKA
                     YNSEVVKNIEIYGEMHRYIPVIAKWAGYSKITEKPVKHQKRKYGVTKFGIERFINGFL
                     DLLSITFVGRFGKRPMHLFGVLGTLSFFFGFVILIYMAYSKLILGEAGMANRPLFYLG
                     ILTVIVGTQLFLAGFLGELIARNSNERNLYNIKETLGLS"
     gene            11333..11938
                     /locus_tag="PLS16_01080"
     CDS             11333..11938
                     /locus_tag="PLS16_01080"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNJ59698.1"
                     /translation="MELILESQNLTDYLAEIPNIIDFNTPLVHQKVKWIKAQTNEPIK
                     QAELAFEIARDEIQHSFDIESKAVSISAEETLNSCEGICFAKAHLLATLLRAMQIPCG
                     FCYQRVLRKGTVESGYALHGLNAVYLPRIGWFRLDPRGNKKGIDSQFLIATEKLAYPI
                     REELGEIDYPNIFTKPLDSVIQSMLNSTSTKELFFNRPTSI"
     gene            complement(11959..12627)
                     /locus_tag="PLS16_01085"
     CDS             complement(11959..12627)
                     /locus_tag="PLS16_01085"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HNJ59699.1"
                     /translation="MLAWDEILIRLITASILGAFIGLERERKTWSAGMRTHMLVCVGS
                     CLIMIVSAFGFQDILGKENITLDPSRIAAQVVSGIGFIGAGTILFQKQGTVKGLTTAA
                     GLWTVAGIGLATGGGLYITAIMTTGIVLIILWILQPIEKKYIRHFVRQTLRITTDIDS
                     KEVDILNILNENEFNIEGLNLIKSDETHILEINFEGANPEKMALLIDQLRKLDSIKEV
                     NWSQ"
     gene            12690..13355
                     /locus_tag="PLS16_01090"
     CDS             12690..13355
                     /locus_tag="PLS16_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59700.1"
                     /translation="MNFSKLLILSLCFIVVACKNKPVSDNQNVLNGKYEIKKAIVETS
                     ITIPNFMEMNIHLMFDDYGKLHKQITKAKQIGEIGAPEIISYSLIQDDYIYNWDNTGK
                     HGTRIHLSPDDEDVFKTGFNFKKVSADFKDKYQLKKIGSKTIKGKDASIYTLVINGNK
                     LKYSIWKNIPLEFEAQLEDRKIQSKLISIDENPNFEQSTFEIPKNITFDDENQGIEGI
                     MEH"
     gene            complement(13357..13944)
                     /locus_tag="PLS16_01095"
     CDS             complement(13357..13944)
                     /locus_tag="PLS16_01095"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNJ59701.1"
                     /translation="MFKLAAEMFMIAKGWRYDVDLIKPIDKCILIVAPHTSAWDFVIG
                     KAAFILGNIPARIAIKKEAFFFPAGIVLRILGGMPIDRKPKDKNSKERLSLVDAIADI
                     IIHSKKIAMIITPEGTRGKTNHWKTGFYYVALKANVPIALGYLNFETKEAIIDKLIHP
                     SGDIDKDMRLIMEYYKDKIHLGKYPNRCTLDERWS"
     gene            complement(14062..15000)
                     /locus_tag="PLS16_01100"
     CDS             complement(14062..15000)
                     /locus_tag="PLS16_01100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNJ59702.1"
                     /translation="MKILLEDKLRKLPWLKSFTLPGFQKIPIYIVLKRLSLEIKSNNI
                     ATRASSISFFFILAIFPGIIFLFSIFPYIPIQNFQANVMQFLASSIPANIFSVLEHTI
                     IDIVGAKKSGSLVSINFIIAFFVASNGVNSILRAFDKSNHTFKKRNFIEKKLTSFKIL
                     FLITFQLILSVILIIKGKEFVQLILNLIGTDDHWMGHAIRTVKSLIILISTFNLYALI
                     YYFGPATKEKYKYFSVGATFATIVSIAFSYIFTIYTTYLNNFNQLYGSLGIIIVIMLW
                     INVNAFVLLFGFELNHSVFINKLEHKQNEEQLLSES"
     gene            complement(15101..15949)
                     /locus_tag="PLS16_01105"
     CDS             complement(15101..15949)
                     /locus_tag="PLS16_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59703.1"
                     /translation="MNKKQIYNVKPLRIKNYNLYLYMRTILLGIIIFINFWSFAQTNK
                     IDTLQAGEHFEFIAPLRILDDQSAQFYYAEANNYFLKQQFTPASFLINKAITMQAENA
                     EYWLLKAWIFSNLEKATALHAAQQAAILKPNDWKMTYCLAYCKQISGDFVGAVIDYSK
                     VIAVQTDNHLAYVGRANCKEALKDYEGAIADYSIAIMFKPTDEKSYLSRGILQYKINQ
                     FYEAISDLSGAIVKMPNNATAYYYRGLAYIELKDYATACTNFTKASELGNKEAKTEIG
                     KYCIRN"
     gene            complement(15927..17078)
                     /gene="hemW"
                     /locus_tag="PLS16_01110"
     CDS             complement(15927..17078)
                     /gene="hemW"
                     /locus_tag="PLS16_01110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNJ59704.1"
                     /translation="MAGIYIHIPYCKHACSYCNFHFSTQTDSSAKMIQAMENEIALQK
                     HFFSKNTKLDSIYFGGGTPSFIDLNYIVKLLKTIYDNYEINEQAEITLEANPDDLTLE
                     YLQDLKQYTPINRLSIGIQSFNYTDLKFMQRVHNATQAKQCILDAQQIGFDITCDLIY
                     GTPTLSNADWKKNIDTLIQLNIKHFSCYALTVEEKTILHHQIQTKKVEAIDEEKIAEQ
                     FTILQDTIAQNGYEQYEISNFCKDKKYAVHNTNYWKNKPYLGIGPSAHSYNGQNRFWN
                     ISNNNLYIKQLLQNELAQSCEHLNNIDHYNEYTLTNLRTIWGVDSQYIETKFGTELRK
                     YFEFEIKPFIDNNWVNLNKTTYTLTNKGKLFCDLITEQVFWIDEQETNL"
     gene            17301..19016
                     /locus_tag="PLS16_01115"
     CDS             17301..19016
                     /locus_tag="PLS16_01115"
                     /inference="COORDINATES: protein motif:HMM:NF015803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HNJ59705.1"
                     /translation="MDKFLKIGFLLLLGLVLQNLIAQSIYLQSHFIESNKSNIKTFKK
                     QADILTYLEELVKKKQNAGYWQYSVDSFYCTKDTCFAQIFEGNKHFLQSNITVSKKTT
                     QLDFLKLKVQLDKQLQKLENSGYPFAQILQDSIHLNKDSISIAYKIDTGQLILVDSFI
                     NHENARISKGFIQNYIGIKSGKPYNESKIVEIDKLLAKLPYTELKQPSDIYFHEDKAD
                     IHLYLKKRKVNKFDFIVGFLPNSSNSNKLMITGEARIHLQNAFKRGEELFFEWTRLHP
                     NTQRLNLKFQYPFLLNTQLGINTSFQLEKRDSTSLDLNLNLGVPYFFKTNNYFKGFYK
                     YAQTIILQADTNFVKSFKRLPGNLDATYNQYGVELYFETLDYLLNPRKGVELRVSTSV
                     GTKKLKTNNQILAINSNDGFNYPALYDSIKKKSLKAELFWNINYYLPFGKRSTIKMSQ
                     NGGSVFNNNILRNELLRIGGNKLLRGFDEESIYASTYIVNTLEYRLLLQKNAYMSVFY
                     DFAYVREQFQQQIKNNFPFGFGIGINFESKIGIFGLSYALGQQKNQPISFRNSKIHFG
                     YVALF"
     gene            19000..19860
                     /locus_tag="PLS16_01120"
     CDS             19000..19860
                     /locus_tag="PLS16_01120"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HNJ59706.1"
                     /translation="MLPYFKQIVLIILCCSIFYSFSADKKDYNPIWVDSILKNKTDSA
                     QMLQLKQSIFIDGQKATPTTVIIQPLRAQQKALDYMYAALLILLIIAFLYIFFGDYFL
                     AVRESLVSIKQYQVYLAANKFDNFFLIVALFLFKIILIAFVVNYFVNIYVSKYYTIFN
                     YKQFTYILSVIAIFFSIKMILEVIVLFIIGQIKTYRLFYFNKLFFDIAFACMVALIIV
                     LMYYNTSTDIRAFLILFGAIYTLYIVYKGFKITQFAKISNKIYFILYLCTFKIMPLML
                     VVKYFWLNLK"
     gene            19866..21071
                     /locus_tag="PLS16_01125"
     CDS             19866..21071
                     /locus_tag="PLS16_01125"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNJ59707.1"
                     /translation="MAKKVTEKKISSTKKVATPKKAAVSTLKKTVKPTKSKDEIKAVE
                     KKTAKTPVKKTASISKEKKTVASTEAKKKNTVKQTIKSSASKSSEKAKSIKVSAKIEP
                     KSTKITKNKIEAEKPKRKYVKKSDKEITNKIIVPDNKVVIKVSSDKPYSVKSILISLP
                     KPESGKSPYIDLANQFKIKLDWRPFTQVEGIKALDFRTQKIYLDQFQNVILTSKTSIE
                     QYFRMLEDMRLKISPENKYFCLNEAIALYLQKYINYRKRKVFFGDGKLNKLIEVILKN
                     KDKAGDATYLIPSSETKDSLLTDTLKSNKIEYKEAILYKTIASDLSDLKNVKYDMLIF
                     FNPSAIDSLYSNFSDFVQDKTRLAIFGNSTGETVMAHKLRIDVFAPTPENPSLVDAIK
                     KYITVGNKK"
     gene            21130..22116
                     /locus_tag="PLS16_01130"
     CDS             21130..22116
                     /locus_tag="PLS16_01130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNJ59708.1"
                     /translation="MKIKRVLFYVLLINWLLPVYAQIKPISSHFNFLNELYNPAFYGI
                     DEQYKFALNYRNQWARLDGAPSTINFLSSFYLPKLKSGIGMNITNDQIGAFKNNQFQL
                     GYNYIIPIKDKLKIGIGAQAGFEFSKLDGTKLITPNGDYSSGINHNDDYLNNQKIKSF
                     RPFLNIGISMNTKYLDLGIAYLNSINNANSFDGILKELKPTYKSVLQTNLKSKIKTGE
                     NFSIEPALMINTDFVNIQTDFQLMFNYRSFVGLGTNIRGYNKNAFESVSTIIKISPLK
                     NKMLGIIYSYDFTFKELGNVNKGSHEITLYYNMRSIGMKTKLPKFINNPRFL"
     gene            22213..23487
                     /locus_tag="PLS16_01135"
     CDS             22213..23487
                     /locus_tag="PLS16_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNJ59709.1"
                     /translation="MKRVTYSLLIVFTLVLSGCKGGKTTDGQLIGSQDRPTWDNNLMP
                     FGMVYVPSGVFHMGPSDQDINYSFNTKMKQISIVGFYMDETEITNNEYRQFVEWVRDS
                     IAHKMLGGDHVQEGENGSQAINWDMPIDWSGATGETEQLESLYYTEADRINGKKELDP
                     RKLIFEYTWYRWKEAAARENKDKPRSQFMDKKQISIYPDELCWIRDFSYSYNEPMTRV
                     YFTHPAYDDYPVVGITWPQANGFCAWRTKLWNGYRESNGMAPVEEFRLPTEYEWEYAA
                     RGGRNLSPYPWGGPYIRNTKGCLLANFKPGRGNYPEDGGLYTVNAKSYWPNDYGLYNM
                     SGNVSEWTSTAYDDASVMFIHDMQPDYRYDAKEDDGSVMKRKVIRGGSWKDIAFYLQT
                     GTRNSEYQDSSKSYIGFRCLLPFLGRGLEDFK"
     gene            23549..24358
                     /gene="gldL"
                     /locus_tag="PLS16_01140"
     CDS             23549..24358
                     /gene="gldL"
                     /locus_tag="PLS16_01140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HNJ59710.1"
                     /translation="MSSFLHSKSFGKLKNMIFGLGASVVIMGALFKLMHWPFASEMLI
                     VGMSVEAFVFAFSALAPAEPTYYWEKLYPGLDDYDAKISAVDVSVAGGAGNKKSLAGE
                     LDKMMEAAKLDQNLVNRFGDNLKGFSENVAKMGSITDATVASNEFANSAKSAATALTG
                     VQTAFTGAAEGMSKLASASGDASKYHEQVQTAAKNLSALNAVYEMELQDTNNHLKSMN
                     KFIGNLDAAMTNLESSIADTDKFKAQMGGLASNLERLNNIYGNMLSAMKAQ"
     gene            24404..25948
                     /gene="gldM"
                     /locus_tag="PLS16_01145"
     CDS             24404..25948
                     /gene="gldM"
                     /locus_tag="PLS16_01145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HNJ59711.1"
                     /translation="MAIPKENRQQMINMMYLVLTALLALNVSAEILNAFKVMNKSLEN
                     SKTIADSNIQATFQAFADKKVKEPANMDVVKYEQQAQQVKTEIDNLMSYISGVKQEIV
                     KRANGPDTEDPDGILSKKDDLEIANAYIVGEGKGEGYKIKSKLEETNNKLMSFVSEED
                     KKKMSELIGLNTNPDKKDGDWVRERYYQMPAIAVYSMLTKLEVDAKNAENQMMAYLYS
                     KIGSMKDLKPKEIVLDEFSAQIASPSAYVLQGEPFKAYVSLAAGSSESSVSITVNGRS
                     VPVKNGVGEFTVGTGSVGEFPVNGVVTVKNNRTGEVSTFKTPDFKYTVAPPFASVAAD
                     KMNVFYIGVENPITVSAAGVSPQKLQVGLSGGGSLSGSGGKYTVKVTQQGKVQVNVSA
                     NGKTYANSEFRVKMIPDPQAEVGNKPGGRMPSAQFKAQNGLIAALKNFDFDARFNVLS
                     FQLFYQPRMADPAIIPNNGPTFNGAAEQAINKAKPGDVFYFEEIKVQGPDGSVRKIPG
                     IAFKID"
     gene            25962..26798
                     /gene="gldN"
                     /locus_tag="PLS16_01150"
     CDS             25962..26798
                     /gene="gldN"
                     /locus_tag="PLS16_01150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HNJ59712.1"
                     /translation="MRYLLSFFFLLSFAPAIFAADATEVNTEIQKLPWSQEISGKRIP
                     MEDVFIRESDVMWYKNVWRVMDCREKLNLHFKWPKRPFIGILLDAIKSGDVPVYSGLD
                     EDFSTPITASEALSVAGGSTDSILVPDAANPDVMVWKVITNEVNWDNVNKFRMKEVWY
                     FDKQHSTMKVRIMGICPLIDNYDPTTGDFRAEVPVFWVYFPSIRKTLVNSEAFNPFPN
                     GVRLNWDQVFQLRLFSSYIYKVDNVLDQRIKDYKSGIDILYESEAQKQQLFQFEHDLW
                     EY"
     gene            26928..27872
                     /locus_tag="PLS16_01155"
     CDS             26928..27872
                     /locus_tag="PLS16_01155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HNJ59713.1"
                     /translation="MKLAFIINGSVRKLDALVEAIHSTFSTYSIEVFISEYAQHYTEL
                     VHKALHNQISHLIFVGGDGTINEGINAFINFYKLDDNSFDWVAIAAIKLAVYPAGSGN
                     DFVKSLYKFKIDSLFALKSLIDSDRVQLVDVGLVNYMDKSKLPSSRFFINITDVGVGG
                     DTVQRKENVPKWLGANFSYMWAITTSITQYKPIAMTAVYDGYNWTGKAMSLVVANGRF
                     FGNGLCVAPDAHLDSGIFELTIVGDISLVEYLKNLGKIKNGIKIIHPEVHYLQAKEID
                     ISSSNSDLMTIDMDGEFIGYAPLNLNCLSKKLTFLSDY"
     assembly_gap    28198..28297
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            28421..29092
                     /locus_tag="PLS16_01160"
     CDS             28421..29092
                     /locus_tag="PLS16_01160"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HNJ59714.1"
                     /translation="MQFAEDIRRIENKINEYQKAGKLMFASSSFQTHSIPMLHIISII
                     DNTIPILFLNTGYLFPKTITYKDQIADLYNLNVIDVKSATPRNMQKDYNGRLLFASDP
                     DFCCHLNKVQPMEPYLQQYDIWINGVRADQNANRANMKEEQATPQRAMRYHPMLPWTK
                     QMIWKYIKENKIPQHPLENEGYTSIGCEPCTRKIDPTMMQDERMARWFGLNKTECGLH
                     TELIK"
     gene            29101..30027
                     /locus_tag="PLS16_01165"
     CDS             29101..30027
                     /locus_tag="PLS16_01165"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ59715.1"
                     /translation="MRVLITGGAGYLGTEIVKKLVQIKTVKEIVVYDNLSKGNYNFFL
                     CTCIGGDNIRFVEGDILDTRKLKKELAKVDVVIHAAAKVATPYTDHDPHLYEQVNHWG
                     TAELSYAIEESNVQKVIHISSVSVYGSSVKSKLIDENTPLNPKTYYSISKMRGESFMA
                     RLGEKIPTHIIRIGNLYGFNQSMRFDAVINRFAFDANFHKKITIHGNGMQERAFINVD
                     KAAKIITNLLVDENKKSSLCNLVDFNKSVMDIAMTFKEIYPELEMQFINQHLKLRNLA
                     IGTNCSMFDEIRTGEVDIKQELQHIIMQFAFH"
     gene            30052..31710
                     /locus_tag="PLS16_01170"
     CDS             30052..31710
                     /locus_tag="PLS16_01170"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59716.1"
                     /translation="MKINYIVSFDESNTHYATINLTISNIQEDYLELKMPVWIPGSYL
                     VREFAKNIDFILDIDTQKRLKKKNKNTWFLEVQDRKTIHLQYKLYCFEWSVRTNHINR
                     EHAFLNGAATFLYIEKYQQLPIEIQFIPSSGWKEISTNLESINEDFWHRIASNFDELY
                     DSVFEIGNQKIYYFDVQQTAYTLAIYGEHDAEISKVISDLQAIIEQQIMIFGQHPCRQ
                     YLFIIHHSENAFGGLEHCHSSVNHIPRNYFHNQVKYQQAISLLAHEHFHLWNVKRIKP
                     SEFIPFDYEHENYTEMLWFFEGITSYYDDLTCYRAGVFSNLIYLKVIEDLLDIVENNA
                     GNDCQTLAESSFDTWIKYYRPNENTGNTQVSYYRKGALVVMLMDIIIQYYSGGKFSLD
                     NLMHSAFKDTLVSNYSGITKPYIIEKLEQICLYDWNTFYENCIESTQPLAISEIFKLS
                     NYQLSISDKNSKTIGVKLKKVGEQFAISSMDKNYFKFNGQLQVDDIVLKIDNQELINN
                     FDEIIEQKAIGKILNFYIRRDGIDKEVEVMLTRSIDKRFTVSPK"
     gene            complement(31766..32098)
                     /locus_tag="PLS16_01175"
     CDS             complement(31766..32098)
                     /locus_tag="PLS16_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNJ59717.1"
                     /translation="MEHITIYIQQENFEFIPLEIPLGVDMSLMEVLKGEEHPIEAICG
                     GMALCATCRVEVLNQDELNLDEKSDSELDILETLPCYTGNCRLTCQIRVSKEIDGMRI
                     KFPEEVFA"
     gene            complement(32119..33120)
                     /locus_tag="PLS16_01180"
     CDS             complement(32119..33120)
                     /locus_tag="PLS16_01180"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNJ59718.1"
                     /translation="MIQTDICIIGAGPVGLFAIFEAGLLKLRCHLVDYLPQVGGQLSE
                     IYPKKPIYDIPGFPSVLAQELIDNLMEQAKPFSPTFTLGERVEGIEKQEDETFILTTH
                     KGTKIHAKIIAIAGGLGVFEPRKPAIENINDYENKGVEYMVIDPEKFRNKKVVVAGGG
                     DSALDWTMYLSNVASELTMVHRGEMFRGAPDSVDKCKQLEAEGKLKMVLNSNITHLHG
                     DEHLNKVTITHKDGDVHDIEVDHYLALFGLSPKLGPIADWGLNIEKNAILVDTKDYST
                     NIKGIYAIGDINTYENKMKLILCGFHEAALMCQSAAEIINGKKPTLKYTTVNGVNAF"
     gene            33332..36283
                     /gene="leuS"
                     /locus_tag="PLS16_01185"
     CDS             33332..36283
                     /gene="leuS"
                     /locus_tag="PLS16_01185"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNJ59719.1"
                     /translation="MIYNFSQIEEQARQKWNYDDKNNSIYKVENNSSKPKYYVLDMFP
                     YPSGAGLHVGHPLGYIFSDIVSRYKRMKGFNVLHPMGFDAFGLPAEQYAIQTGQHPAI
                     TTQKNIETYRSQLRKIGFCYDWSREISTCEPQYYQHTQWIFLQLFNHYYDKKQTKALP
                     ITTLITVFENEGNTKIQAACEDKVIKFSAAEWKSFSSTQQQQILMDYRLAFQKDSFVN
                     WCPALGTVLANDEIVNGVSERGGHPVEKKKMSQWFLRITAYAERLLQGLDTLDWSDAM
                     KEMQRNWIGKSKGAMVAFQLKNHQDKLDVFTTRVDTIFGVSFMVIAPEHDLVKQITTE
                     EYKQAVDEYVKKASAKSDIERQAEVKETTGQFTGAFVFHPFTNAEIPVFIADYVLAGY
                     GTGAVMAVPSGDQRDWLFAKKFDLPIIAISDAQTKLENAADDTKEGKYINSDFINGLT
                     YNEAVDRLITKLEEQKIGFGKIQYRMRDAGYSRQRYWGEPFPIYYDEQGIIHTVDEDK
                     LPLTLPSVESYKPTGTGQSPLATASNWVHQFGANTRMETDTMPGYAGSSWYYLRYMQP
                     DNSNEIVDFKVQDYWKNVDLYVGGSEHAVGHLLYSRFWNKFLKDIGKVSAEEPFQKMI
                     NQGMIQGVSKFVYRISSKFQFDSSKAKISDTDAFILWEKYFGNLFISKNIIDKVNLLD
                     FLKIKLQDGLQILNDNGLYLEFDSISLLKLHIDVKYVKNDILDVERLKTEDHNYKDAV
                     FITEEDGNYYCGSEVEKMSKSKFNVVNPDEMIAQYGADVFRMFEMFLGPIDTAKPWDT
                     NGISGVSGFFRKFWRLFFDDNGIKKYADTEATADELKILHKCIKKINQDIEQLSLNTC
                     VSTFMIAVNDLGKLPSISKSTLVELSKLLAPFAPHTSEVLWSELGENNCIVNENYPVH
                     NEAYLIESSYTYPIQVNGKHRANLEISLTATQEEVEQIVLGDEQITKYLEGKTPKKII
                     LVKGRIVNIVI"
     gene            36351..36929
                     /locus_tag="PLS16_01190"
     CDS             36351..36929
                     /locus_tag="PLS16_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59720.1"
                     /translation="MDEIKYNYRKSILRHYVNYTLTKDGLLITSPDYKDRIIPYHEIT
                     SLRFEYLLNNRYDSNRYSCTIYTIDNKEYQILSTSYAGIANFENQAEPYVVFVKGLLA
                     IVRKINPNSKIYLGQSKIKYFGNMVLLILVLLLLLSVMSYFPVSASFSIVFKLIMIVF
                     FSFYMVLSFTKNYPRELEIGQEIDESVLPKIK"
     gene            complement(36938..37930)
                     /locus_tag="PLS16_01195"
     CDS             complement(36938..37930)
                     /locus_tag="PLS16_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl transferase"
                     /protein_id="HNJ59721.1"
                     /translation="MINASKLEEKIKSNNYDFDDLALEIFYFQYKYNKVYREFVDLSN
                     IKIDSIQSIEQIPFLPIQFFKTHNIYCGSEQPNFYFESSGTTQSINSKHYVKDIELYT
                     HIFKNGFQDFYGAIQDYNIIALLPNYLERQNSSLVYMFDHLIKLSNSPHSGFYLSDFE
                     QLNHVLQQTKTDNRKTLLLGVTFALLDFANAYPQDLSHCIIMETGGMKGRRKEITKQE
                     VHATLQKAFNVDAIHAEYGMSELLSQAYSKENGQYHTIPSMKILCRDIYNPKQILNFS
                     ERGILNIIDLGNLYSCSFIETQDMGIAYSNTHFEVLGRIDYAEIRGCNLMVSDL"
     gene            37929..38483
                     /locus_tag="PLS16_01200"
     CDS             37929..38483
                     /locus_tag="PLS16_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59722.1"
                     /translation="MTTKLSNISLKFFILLVCLFCFLACFKEAFYPNTPTVKFNKYDL
                     ENNAYQYNSSKGSLIVDFTDGDGDLGVVYKDTLIIVGTDTIKKIPISGKVYYTIPKYD
                     SIRNYVIDEYAFPEISSAYNGSKNGILEIKFTSIFDEVKYSKITNSFPGIQRDTINFK
                     LWMKDRAGNQSNIITTPDFIINYN"
     gene            38488..38739
                     /locus_tag="PLS16_01205"
     CDS             38488..38739
                     /locus_tag="PLS16_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59723.1"
                     /translation="MRILGQIPHPIIKISVLALNDKFILKLEYDLLEQVYKIRNCDEI
                     SNFGTLEELVDEKFLESCIKRFNEMNKDLYDTFERNNIM"
     gene            complement(38839..40419)
                     /locus_tag="PLS16_01210"
     CDS             complement(38839..40419)
                     /locus_tag="PLS16_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNJ59724.1"
                     /translation="MKKLIFFLCIVFSTFIIFAESGKKENTKKNTKALYNIETKKYLK
                     DWAFIIHGGMTSPFTDIRSYDWTRSAKSPSEIQWAAGLGLTKMFNSAFGINIDYTLGR
                     ISGKTAEQGGFAEERQYWKQMGFEEPVYFRTSVFHQASVNFYIDWLGLAFGYNKFIKS
                     QIKQKPVKPRIFALYNKIGIGIWRGESNIYNIGDDLAIKNAAYLRGYTNRFTETIFPF
                     TTGMKFKVSKAFDLGLEGTFVFSNSDKLDAFNFQNNYNTLTGQTINSLSKINRDAYLY
                     MNINFTYKFGRIGSQAEHIEWVNPMEAIMAYQNQEKPEPMVLLDTDGDGVFDIIDEEP
                     ETPKGAKVDTKGRTLDSDGDGCPDHLDPEPFSNPRFKIVDCINIIDTPKIIIEKKEIQ
                     TNTIETIKETTIKKEDTWKLTSIYFDYDKYNITPTAATELKKIAAVMQKKSDLIVEVQ
                     GHTDTRGSLEYNQKLSENRVNYAINYLIKNFGIKEDRFKRLPLGKIDPTVKDAHTENQ
                     HQINRRVDFKPVNMQEGE"
     gene            complement(40481..41134)
                     /locus_tag="PLS16_01215"
     CDS             complement(40481..41134)
                     /locus_tag="PLS16_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59725.1"
                     /translation="MKNYFLLTLLALFIVSYAQDNSIIDTPNKSDFSYNNKTSDENQK
                     LDTLYTYDVNLYETSTPFGIKYTVNGKEITKKKYDKYRQYWQAMDGCTPCLLRTFDED
                     DKIKYEAFQYIDCLCGEYTEYYKDGSTKVEGQFMKNPNGTWENIKTQQFCNRRDGKWT
                     YFLPNGTFEKLEVYKNGVLIETKGNSELVPAMNVKNREDSTQDSEIEKKKPFFKRKN"
     gene            complement(41209..>41915)
                     /locus_tag="PLS16_01220"
     CDS             complement(41209..>41915)
                     /locus_tag="PLS16_01220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNJ59726.1"
                     /translation="IKSALKIKNTYTFNKQSIKQWLLTSFKINLLNNHIIDRYFTAVK
                     KLNVINDGKGLDYFIPEADEILIANLPFTHIAGYCVIVVGAKHFTKTIPLSKLKVLCQ
                     EIKMPIILVGGTDDYERAEQLASIDDFKIYNACGKLNINNSASIIKKAKFVITGDTGM
                     MHIAAAFQKRIISVWGGTHKNLGYYPYQNNNNTVYIENESLSCRPCHRFGRANCPKKH
                     FKCMNDLDMQKIIELV"
BASE COUNT        14245 a         5972 c         6663 g        14935 t
ORIGIN      
        1 gaggtccttt aatagataaa tatgcacaaa gtggtgatgt tttaaaattt ccattttcag
       61 aacctaaaat tccagaattg aattttagtt ttagtggatt taaaactcaa gtcttatatt
      121 tcttacaaaa gcaagaaaag ttaaacccag aatttgtaaa agaaaatatg gctgatatct
      181 gtgcttctat tcaatataat ttgataaaaa tattgatgag taaactcaaa atggcaagta
      241 aacaatttgg tattaatgaa attgctatcg caggtggagt tgccgcaaat tctgaattga
      301 ggaaacaatt aaaatcaacg gcacaacaat tagattggaa ggtatatatt ccagattttc
      361 aatattgtac tgataatgct ggaatgattg cgattacggc ttattataaa ttcttaaaga
      421 aacaattttg tgctatcaac ataccaattc aaccaagatt aaatttttag aatgacaaga
      481 atcgacatca cagttccaga caagatatta tttgaaactc attttttcat caaagaagaa
      541 gacattaatt ttgcaaacca catgggcaat gaacgtatat tggtttttgc taatacagtt
      601 cgaacaaatt tttataaaca cctagatttg ttagaagggg attgggcaaa tggacacggg
      661 acaattgtcg cgaatcattc agtaaaatat attaccgaag gttttctgga tgatgaaatt
      721 atttgtaagg tgggtgtcaa tgaattaaca acatgtagct ttgatatggt gatgcaattt
      781 attaaaaaag aaactcaaaa aaccttagca ttggtaagaa gtgcagttgt ttattttgat
      841 tataatgaaa gaagaattag agaattgccc caaagctatt tagataagtt tttgtaataa
      901 atttttgagg atttatttat cttctataat ttttatttcg tctgctgtta agtcgtaaag
      961 ggcataaatg atttgattga ttttatcttc agcatattca atgcgatgat gtagctgttc
     1021 tttttgatga gataaagttg cggtttgttt ttctttattg agttgtaaaa tttgttcaac
     1081 tagtttgata atctcatttt cttgtttgcc agaaatattt tttggaattg gcaattgctc
     1141 aacgtgttgc tttttcactt ctgccagtgc ttctccccgt tcgggattga tttgaaaata
     1201 gtaaaaatct gtcagttttg agttcaaaat tccgagaata aatttgtggt ctaatttgtt
     1261 ggaaattata atatgtaaat tttttctaca gataatatct ttccctataa tagttgcaat
     1321 aattctgtcg cctgtttgac gaacaataat cttttcattg gcttcccata ttttacgatt
     1381 tcttggtgca gcaagccact ctccataatc aacccaataa ttgttgttcc aaatatttat
     1441 gtatctattc attaaacttc ctcgcataag tggaagccaa ttttttcctt taggtttttg
     1501 aaaatcatta ctctcaaatg gtttcacttt catagtttct gccgtttgtg ctggatttcc
     1561 tttccctttt tcatatagtg taattcctga atatgatttt gcaatttctg atatttgaat
     1621 tgatttactt tttatttttt caaataaata cttttcttct tcatttgcca aaatattgat
     1681 aatgtctcca ttttctggta aattattttg gtctatactc tgaaattgtg ttattgtttt
     1741 gtgcttgagt atatcaattt caagttctgt atttttgata taattattct tctcaaaaat
     1801 caatacatga gtatctacaa cagcattaaa tactttgtct ttattgtgta atattctttc
     1861 ccaataatag taattcatta aatagtttct aatattcctg aacttaatac tttgcaacca
     1921 tgtatttgga ataattacac ctaatttccc accttccacc aaaaggtttt tatattgctc
     1981 taaaaacaat aaatacaaat cttgctgata atcttgatgt ataaattttt tattgaaata
     2041 attaaagata tgtttgtctg caacttttac ataaggcgga tttccaataa taacatcaaa
     2101 accaccattg ttttttttat attctttagt tggttcattt agtaattggg ataacaattt
     2161 gtctgtatca tcacttagtt ttttaacttt ttgaaattgt tttttatatg gtaagcgttc
     2221 gtcaatgggt acattcctat tgaaaacttc agggaatgct ttttcccaag agaatggctt
     2281 tacctttttt tcttcgccaa agtcaatttc attatcaaaa taatctaagt caattaaaga
     2341 gtttccactt ttaatattgt tatccaaagt tggtaaaata cggtcgtgaa acaattttgc
     2401 ttgcgcttct atactttctt tggtttcgcc ttccatacat tttaaaagta atgaaagttt
     2461 ggttacttca acagcattgc tatccaagtc cactccataa atattattga gcaaaatacg
     2521 tttcttttca gcagtggtca attcgccagt tggagtcaaa ggattgtctt tgttgccttt
     2581 gtaaattttg ccgttttgcg tataaaaatc tttgtgccag ttcaatagaa attgatatgc
     2641 accaagtaaa aaacttccac ttccacaagc tggatcaaca atttttattc tgcttacttc
     2701 tttcggcgta atattttccc ctcctttgga ggggttaggg gaggttagtt ttccaattgt
     2761 gttgtttaca atataatcaa caatatattg cggtgtataa taaacgccac ctgcttttct
     2821 tacttctggt ttttcttcaa ttactgcttt tccgtttttg gaaagtgtga tttgtttacc
     2881 taaaaattgc tcataggcac ttcctaaaat ctctacggat aaaactgaaa attcgtaagg
     2941 acaaattgga taatacaatt ctgaaatgat ttccttaatg actttgttgt caatcgtgat
     3001 tttttcactg attttatctt tttggaaatc aaataagcct gaattatatt tttgatctgc
     3061 ttgataaaaa cgatacaaaa gattttgata aaaagtccca cttttgatgt cggttgctaa
     3121 gcctccataa ggttcagcac ttcggtcttc tgcaattctc aaaaatatta ttctgtcaat
     3181 cgtatgttga acaataaagt tgagttcttc ttcagaaata tctttgttgc gaagtgcaat
     3241 atttacagcc aagtccactc gccatttatc caaggattgt aaaaactctt tatcaacggt
     3301 gcttgttcct tttttgtttt tgtcgctgct gatgtatttg tcaaaactgc ctttcagcac
     3361 tttttctttg ctgaaagtat cccaaataaa atcaaactca tttaaatact ccgtgtattg
     3421 taaatatttt attcttccat tgctggcttt gtctgtgggt tttggtttat tggcacaatc
     3481 gtaaatagca aattcttcaa aatctgaaat tacagaaatt ggcattttgg cactccaacc
     3541 atatcttcgt acttgatagg ctggtgcaat atcgtctttt acagatacac ttggtttctt
     3601 ggcttctaaa aagaataatc ttttacctcc aaccaatctg aaagaatagt cgggagcttt
     3661 tgtcgcactg cctactttta tacggtcttc atgaatgact tctctgtaac tttctgcata
     3721 gccgttttca ttatcaatat cccaaccaag agctttccaa aatgggtcta taaaatcacg
     3781 tctagtttgc gtttcgttat agtctgcttt tttgtaaaat tctttttgtt catcaaatcg
     3841 ttctactaaa cgtgcaattt tgttatatgc ttgttctttt gaagacataa gttcaagata
     3901 acaatttatt ttgtttaatt gtagattatt tttgaaaata taaaattata ttgttcaaag
     3961 aaaattaaat gcggagacgg cgagattcga actcgcggta cccttttgga gtacacacac
     4021 tttccaggcg tgctccttca accactcgga cacgtctcct gatagaattt ctaaaaaaaa
     4081 attaaaaacc ctttatatca ttaaaataaa aaacttttat gcttttccag tcaatacagc
     4141 tcttgaaatt acaattttct gtacttctgt tgtgccttca tagatttgcg taattttcgc
     4201 atctctcatt aatctttcta catggtattc tctcacatat ccatatccac catgtatttg
     4261 tactgcttca attgttgttt tcatagcaac ttctgaggca taatattttg ccattgcaga
     4321 tgcaactgca taatcttgcc cactatcttt caaccaagct gctttataaa ctaacattct
     4381 agctgcttca atttctgtag ccatatctgc taatttgaat gcaatagctt gatgatttga
     4441 aatttcagtt ccaaatgctt ttcttgtttt tgaatattct aaagctaatt cataagctcc
     4501 agctgcaatt cccaaagctt gtgctgcaat tccgatacgt ccgccttcta aggttttcat
     4561 agcgaaagta aatccaaaac catcatcacc aattctattt tctttaggaa cttttacatc
     4621 attgaataat aaagaatgtg tatctgatgc acgaattcct aatttatctt ctttagcacc
     4681 aatttcaaaa cctggcattc ctttttctac gattaaaaca ttgatgcctt tatgtccttt
     4741 ttctgggtgt gtttgagcaa tcactaaata agttgaagct tttccaccgt ttgtaatcca
     4801 gttttttgta ccatttaata agtaataatc tcctttatct tcagcaattg ttctttgtga
     4861 agtcgcatca ctaccagctt ctggctcaga taaggcaaat gcaccccaaa tttctccttt
     4921 tgccaatggt actaaatatt tttgtttttg ttcttctgtt ccgtattttt ctaatcccca
     4981 acaaactaaa gagttgttta cagacattgc aactgagcaa gaactatcta attttgatat
     5041 ttcttccatt gctaaaacat aagaaattgt atctaatcca gttccgccat actttggatc
     5101 aaccatcatt cccataaagc ccatttcaga catttgcttt aactcctcag ttggatgacg
     5161 catatctcta tctctttcta tcacttcttt tttcaataca tcttgtgtaa actttctagc
     5221 agcttctcta atagataggt gttcttcggt aagttgaaaa ttcattttta tcaattttgt
     5281 ttgcaaaagt acaaaattaa ttaaagagaa caaagcctat tttattaaac ataatcggca
     5341 tcttgtcccc aacgatattc cattagatat tgtagtgctt tttcaacgct caaatcttca
     5401 taaattactt tgtaaatatt atcaataata ggtgttctga tatttttaga ttcagagatt
     5461 ttcttactga tattgaccgt tttaatacct tcagccactt cagatgaagt tgcaataata
     5521 tgttctatgg tttcgccttt tgctaatcta gctccgacag tataattacg acttagtgga
     5581 cttgtacaag tggcaaccaa atcgccgata cctgccaaac ctaagaaagc atttttatta
     5641 ccgcccattg ctgttgcaag tctgatgatt tctccaatac ctcttgtaat taaaaatgcc
     5701 attgcatttt gtccaaagcc taatccgcct aaggctccag ccgctaaagc cattgagttt
     5761 tttaaaacgc ctgctaattc aacagaatag atatcgttat tgccataaac ttgtaaacgt
     5821 ttacttctaa tggcactttc gccttctcgt atgacttcgt caaatttact agcaataaca
     5881 gtagctgctg gttgcaaatt agcaatttcc ctagctaaat tagggccaga catgcaacct
     5941 actcttttta cacaagtttc ctccaatatc acctgactca tggtttttac atctgttaaa
     6001 ctgattttta aatctttatc aactaatgaa gtaccatctt ctttgtgaat atcaaaacct
     6061 ttggtgcaat gaatgatgat gtggtcgggt tttaaatgag gtgcaaatcc ttgaatggtt
     6121 gttctgaatt tatcagaaga aagtgcagga aaaataagat agcaattgtt tgttacatct
     6181 tgaatgttat tggttggaat aatattttcg tgtaattgtt ggccaatatt ggaacgttca
     6241 gctttcattt tatcgacagc ttcgcttctg ttggaataca atataacacg accattctcc
     6301 gctaataaat ttgctactgc agtaccaaaa ctaccagctc caatgacacc aacaggtgcc
     6361 ttatctaatg aatttctcaa gtccatatcc ttccattctg ttatgataat aatataataa
     6421 ttgcaaatcc tcgctggtaa tattcccttt tttgttttta agtaaaggta aatttgtatg
     6481 ataaatacta atgttttgaa tgcctaattc aatcacttca tctaattctt tggtaatgag
     6541 attttgggat agctttagtt ttcctttatt gttcaaatga tacaattcat ctaggattaa
     6601 tttcatattc attttaaaat cttcccaatc aatttctcgg tcttcttcag gtaatctaag
     6661 tatggtgtaa atgtcatttg ttccaatttt tttctttaat aactcaaaag ctacaaacgg
     6721 aacaacatgt gctgttttta caacattatt cttatgaaaa ctatcaataa ttctttcgcc
     6781 tagaaattga gtatatatca tttctctttg attgtctcgt ttaaaaatgc catttgtata
     6841 gaagtattta tcaatctcta aaatattatt gttgtggtct aaactattgc catcatcatc
     6901 aatattatta ccaaaaacat ccattggttc gcccaaagaa acatgcacat ctgaggattt
     6961 tgaaaatgtt tcccaaagga aattccatat tgctttaaaa ggtacttgtg ctggttcttt
     7021 tactaaatag ttttcttttc cagtgcgtct tagataatca ctaaccaaag aagatgcttc
     7081 taatacgaaa ttataactta aaactaaagg aactacataa atttttgggg ctaaatctac
     7141 tggattattt tcatagtgtt ctcgttgggc atctatcaca gttcccaata aacctaattt
     7201 taaattggtt tcaatagcac catttctaga acgagtacca cctgggaaaa atatggtatg
     7261 tgctccagcc attacattgg ttttggagaa ttgttttaaa gtttctaaat aaataggatt
     7321 tttctttctt ctatctaatt tatacgctcc taaatttccc ataaatagtg caaaaaattt
     7381 agagttgaat aaattcaaac ctgcaccata ttggaatgct ggcataccga tataatccat
     7441 cgcaaagcct atcattgcag aatctaaatt actgaaatga gtcggaacta aaacaaccgt
     7501 tccttttgtt gctaaagctc tgattttttc aactgcacca gacacgatta atttgtcttt
     7561 taaagcatct tgaggtcgaa aaagttcttt gaaactacta aaatgagcat tgaataaacg
     7621 agcgaaaatt cttcgcaaca attgtcttgc aaaccaaaat aatttaggac tgaaattacc
     7681 catcatttca tgggcatacc tagaaacaat tgcttctagc gtttcattat tccttttagc
     7741 aactaattct gagtcttgag tttcattagt tacagaatgt aagacttttt tcttgattcc
     7801 attccaaaat tcagcttcat cttcaggatc aacttcccaa ggttctgtct taattctgat
     7861 tcgttcaaga tatagtgttt tctgtacttg ttctcttatc ttttggtcat ttggatagtc
     7921 tttttttaat tgtaatatcg cttcactaat tgtttcttgc aatattctgt ctttttgcaa
     7981 agaaatttta ttaattggcc aatcttcaat tctatctatt accggtttgt aaatcatagt
     8041 tcttttatta agaattctgt aaaaatttac ggaacaaaat actaatatag tactttttca
     8101 attagtggaa ataaaaaata acgatacctt tgtattgtga atgtaaaagc tcaaaaaagt
     8161 tacggacaac attttttaaa agatacacaa gtattacatg ctattgttga tgccgtaaat
     8221 caaagcagac aagtattgcc tttgattgaa ataggccctg gaatgggtgc tttaacccaa
     8281 tatcttatac aaattgaagc accattgact tgtatagaag tggataatcg gtgtgttact
     8341 tatttagaag aaaatttctt gaataaatcc aagcaattca atttattaaa tgctgatttt
     8401 ctgaaagttg atttgaataa gttgatagct gaagaagcat ttgtagttgg aaactttcca
     8461 tataatattt caactcagat tatttttaag ctattagatg tttatcaaaa agttcctgtg
     8521 gttattggta tgtttcaaaa agaagttgca gaacgattgg cggcaaagca tggtaataaa
     8581 gtttatggca taacgagcat attaactcag ttaatttatg atgttgaaat tttgtttgat
     8641 atagctccag aaagtttttt accaccacca aaagttatga gttctgtaat taaattaact
     8701 cgaaaaaaag atattccaac ggattttaat tattcttttt ttaaaagtat tgtaaaaacg
     8761 agctttaatc aaagacgaaa aatgatgcgt tcttccttaa aatctgtagt taataaagaa
     8821 tttttgcaag ataaatattt tgatttgcga ccagaacaat taagtgtagc agattttatt
     8881 gaattggcga aaaagattga aaattatgga aaataagaaa gcttttataa cggctgatat
     8941 tcatccattt ttgcagcaac aattagaaca aaaatatatt gtagatgtcg atgttgcggt
     9001 aagtcgtgag aaacttttgt cagtaattga acattatgaa atattggtta ttacgaccta
     9061 tttaaaagtg gacaaggaac taattgacaa agccttaagt ttaaaagttg ttgcacgtgt
     9121 gggaagtggt ttggaaaatg tagatgttga ttatgcaaaa tcaaagggaa taaatgttat
     9181 caattcgcca gaaggaaatg cgaacgcagt tggtgagcat tctttaggaa tgttattgaa
     9241 tttgatgaat aaaatcaata tcagtaacct agcattgaag caacaaattt gggatagaga
     9301 atcttttcga ggtgaagaat tagatggaaa aacgatagga ataattggat atggacatac
     9361 tggaccagcc tttgcaaaaa aattagcagg ttttgaggtc aatattttag tccatgatat
     9421 tgtatccaaa gagcaggtga aacaagtgga tattgaaacg attcaaaaag aatgcgatat
     9481 tataagtttg catgtacctt atacgtcttt aacacatcat ctaattaatt tagaatttat
     9541 tagaaaatgt gataaaaagc ctattattat taatacttca agaggaagtg tggtggacac
     9601 tccagcaatt attcatgcat taaaacatca aaatattaga ggattttgtg tcgatgtgtt
     9661 tgaagatgaa ccaataggag caggaaaagt ttatcctcca atgttatatc atcaattatt
     9721 gaattttgag catgtagtag ctacttctca tgttgcaggt tggacaattg aatctaagtt
     9781 taaattagta gaagtacttt ggcaaaaaat gcaagatttt attgcataaa acctataaga
     9841 aaattttgta tttttgcatt tagaatttac gatggcagaa aataatcaag caaatcacgc
     9901 acaaggaatg cttacaggta ttatgattac tttgtttgca gcattaatag gtgtgttggc
     9961 gtatggtagt gtttttacct tggctcatta tatgggtaag gattttggac aattacaaaa
    10021 tacagttttt acagtcttgt ctgtaattgt agctgtggta cttgctcaat tattatcaag
    10081 aggtattcaa ggtaatagat taagttttat gcaagcattt ttgagcggtt ggatggcaag
    10141 tttgatatta ggtatgttta tcaatttttt ctttactata tttattcaaa ttacaaaaat
    10201 gccacctttg caagaaggaa gttttgcacg aatgatgatg ttgtttagta tgttgggtat
    10261 ttttacatct ttaattttat cacttattac taaaaaaaat tagcttggat atttcagtag
    10321 tcataccttt attaaatgaa gcagaatctt tgcctgaatt attttcatgg attgaaaagg
    10381 ttatgcaaga aaataatttt acttatgaaa ttattgcggt tgatgatgga agcacagata
    10441 attcttggga gattattcaa aatttatcca aagaaaatca aaatataaaa ggaattcgtt
    10501 ttcagcgtaa ttatggaaaa tcggcagcat tacaaacagc ttttcaagca acaaatggaa
    10561 atgttgtaat tacaatggat gctgatttac aagattctcc tgatgaaatt cctgaattgt
    10621 atagtatgat agtaagggaa aaatatcatt tagtttcggg ttggaaacaa gagcgttttg
    10681 ataataaatt gacgaaaaat ttaccttcta aattattcaa ttttgttaca cagcaagttt
    10741 ctggaattaa aaatctacat gattttaatt gtggattaaa agcatataac tcagaagttg
    10801 ttaagaatat tgagatttat ggcgagatgc atcgttatat tcctgttatt gcaaaatggg
    10861 ctggttatag taaaattaca gaaaagccag taaaacatca gaaaagaaaa tacggcgtta
    10921 ctaaatttgg gatagaacgt tttattaatg gatttttaga cttattgagc attacttttg
    10981 ttggaagatt tgggaaaaga ccgatgcatt tatttggtgt tttaggaact ttatctttct
    11041 tttttggatt tgtaatatta atttatatgg catattccaa actgatttta ggagaagcag
    11101 gaatggcaaa tcgacctttg ttttatttag gaattcttac cgttattgta ggtacacaat
    11161 tattccttgc tggctttcta ggagaattaa ttgctagaaa tagtaatgaa cgaaatctct
    11221 ataatatcaa agaaacttta ggtttgtcgt aataattttg attcataaat tatcttaaaa
    11281 atttcaatag ctaagcttta gattttaact ttattgcata gaatttgtag aaatggaatt
    11341 aatattagaa tctcaaaatt taactgatta tttagctgaa attcctaaca taattgattt
    11401 taatacgcct ttagtacatc aaaaagtaaa atggattaaa gcacaaacaa atgagcctat
    11461 aaaacaagcc gaacttgctt ttgaaattgc tcgtgatgaa atccagcact cttttgatat
    11521 tgaaagtaaa gcagtgagta tttcagcaga agaaacctta aattcttgtg aaggaatttg
    11581 ttttgcaaaa gcacatcttt tagcaacttt actcagagcg atgcagattc cttgtggttt
    11641 ctgttatcag cgtgtactta gaaaaggaac tgtagaatct ggttatgcat tacatggatt
    11701 aaatgctgtt tacttaccaa gaattggttg gtttcgttta gacccacgtg ggaataagaa
    11761 aggtatagat agtcagtttt taattgctac agaaaaatta gcttatccaa ttcgagaaga
    11821 attaggagag attgattatc caaatatttt tacaaaacca ttggatagtg taattcaaag
    11881 tatgctcaat tctacgagta caaaggagtt gtttttcaat agaccgacaa gtatttaagc
    11941 attttcttat tagaaatttt attgactcca atttacttcc ttaatggaat caagtttgcg
    12001 tagttggtca atgagtaatg ccatcttttc tggatttgct ccttcaaaat ttatttctag
    12061 aatatgtgtt tcgtctgatt tgattaaatt taaaccttct atattaaatt cattttcatt
    12121 taaaatattt aaaatatcta cttctttaga atcaatatca gtggttattc taagcgtttg
    12181 tcgaacaaaa tgacgtatgt atttcttttc aataggttgt aatatccata ggataatcaa
    12241 tacaatgcca gttgtcatta ttgcagtgat atataagccg cctccagttg caagaccgat
    12301 tcctgcaacc gtccacaatc ctgcggctgt tgttaaaccc tttacagttc cttgtttttg
    12361 aaatagaatt gttccagctc ctataaaacc aattccacta accacttggg ctgcaattct
    12421 tgatggatct aaagtgatat tttctttgcc taatatatct tgaaaaccaa atgctgaaac
    12481 tatcataatc aagcaagacc cgacacaaac aagcatgtgt gttcgcattc cagcagacca
    12541 tgtttttcgt tctctttcca atccgatgaa tgcacctaag attgatgcag tgattaagcg
    12601 gattaatatt tcatcccaag caagcatatc catgtatttc taactcaaac ttacttattt
    12661 tttcttattt ttgacctata ttctagctta tgaatttttc taaattgctt attctttctt
    12721 tgtgttttat tgtagttgct tgcaaaaata aaccagtatc tgataatcag aatgtcttaa
    12781 atggaaaata tgaaattaag aaagcaattg ttgaaacaag tattactatt ccaaatttta
    12841 tggaaatgaa tattcatttg atgtttgatg attatggaaa attacataag caaattacca
    12901 aagccaaaca aataggcgaa attggagctc ctgaaattat ttcttattca ttgattcaag
    12961 acgattatat ttataattgg gacaatacag gtaaacatgg tacaagaata catttgtctc
    13021 cagatgacga agatgttttt aaaacgggat ttaattttaa gaaagtaagt gctgatttta
    13081 aagataagta tcagttgaaa aaaattggtt caaaaacaat caaaggaaaa gatgcatcta
    13141 tctatacttt agtaataaat ggcaataaat tgaaatattc tatttggaag aatattccat
    13201 tagaatttga agctcaattg gaagatagaa aaattcagag taaattaatt agtattgatg
    13261 aaaatccaaa ttttgagcaa tctacatttg aaattcctaa gaatattaca tttgatgatg
    13321 aaaatcaagg tatagaagga ataatggaac actaaattaa ctccaacgtt catcgagcgt
    13381 acaacgattt ggatattttc ccaaatgaat tttatctttg tagtattcca ttattaaccg
    13441 catatcttta tcgatatcac cacttggatg aattaattta tcaataattg cttctttagt
    13501 ttcaaaattc aaatatccca aagcaatagg aacgtttgct tttaatgcaa cataataaaa
    13561 gccagttttc caatgattgg ttttccctct tgtaccttct ggtgtaatta tcatcgctat
    13621 tttcttgcta tgaataatta tatctgcaat ggcatctact aaacttaatc gttctttact
    13681 atttttatcc tttggttttc tatcgatagg cattcctcct aaaattctaa gaactatacc
    13741 tgctggaaag aaaaatgctt ctttttttat tgcaattctt gcaggaatat tacccaaaat
    13801 aaatgcagct ttcccaatta caaaatccca agcagaagtg tgcggagcaa ctatcaagat
    13861 gcacttgtca attggtttta ttaaatcaac atcatatcgc caacctttgg caatcataaa
    13921 catctctgca gcaagtttga acatagtgat ttatttattt tgcaaaatta aataatgtaa
    13981 atcactaagt attcactata aataattagt gcggtttaaa aaaatcttat cgtaatggaa
    14041 aattattttt aaaatggatt attatgattc tgaaagcaac tgctcttcat tttgtttgtg
    14101 ttctagttta ttgataaaca cactatgatt taattcaaaa ccaaataaca gcacgaatgc
    14161 gtttacattt atccaaagca taattacaat gataatcccc aaagaaccat acaattggtt
    14221 gaaattattt aagtaagtcg tgtaaattgt aaaaatataa gagaatgcaa tggaaacaat
    14281 agttgcgaaa gttgcaccaa cagaaaagta tttgtatttc tcttttgtag caggtccaaa
    14341 ataataaatc aatgcataga gattgaaagt agaaattaaa ataattagag attttacagt
    14401 tctaattgcg tgtcccatcc aatggtcatc tgtgccaatt aaatttagta tcaattgtac
    14461 aaattctttt ccttttataa ttaagatgac agataagatt aattgaaaag taattaaaaa
    14521 tagtatctta aatgaggtaa gtttcttttc aataaaatta cgctttttaa aagtatgatt
    14581 gcttttgtca aatgctctca aaatagaatt taccccatta gaagccacaa aaaaagcaat
    14641 gataaagttg atagaaacta aacttccact tttctttgca ccaacaatat ctataattgt
    14701 atgttccaaa acactaaaga tgtttgcagg tattgaactt gctaaaaatt gcatcacatt
    14761 agcttgaaaa ttttggatag ggatatatgg gaaaattgaa aataagaaaa taatacccgg
    14821 aaatatcgcc aaaataaaaa agaaggaaat acttgatgct ctagtcgcaa tattattgga
    14881 tttaatttct agtgaaagcc tttttaatac tatgtatata ggaattttct ggaatccagg
    14941 aagtgtgaaa ctttttaacc aaggaagttt tctgagttta tcttctaata gtattttcaa
    15001 tagattcttt tagttctgag tataaaaata caaattttta tgccaagtgc aaaattttac
    15061 gaattgtaga aagtctatct aatttgaatt tgttggtaga ttaatttcta atacaatatt
    15121 taccaatttc ggttttagct tctttattgc ctaattctga tgctttggta aaattagtac
    15181 aagccgttgc ataatctttc aattcaatat aagccaagcc tctataataa taagctgtag
    15241 cattattagg catttttaca atagcacccg ataaatcgct aatcgcttca taaaattgat
    15301 tgattttata ttgtaatatt cctctactta gataagattt ttcgtcggtt ggtttaaaca
    15361 ttatagcaat actataatct gcaatagcac cttcataatc ttttagtgct tctttacaat
    15421 tggctctacc aacataagct aaatgattat ctgtttgaac cgcaattacc ttactgtaat
    15481 ctattacagc accaacaaaa tctccagaaa tctgcttaca atatgccaaa caataagtca
    15541 ttttccaatc atttggtttt aatattgcgg cttgttgtgc agcatgcagt gcagtagctt
    15601 tttctaaatt agaaaaaatc catgctttta ataaccaata ctctgcattt tcagcttgca
    15661 tagtaattgc cttattaatc aaaaaactag caggtgtaaa ttgttgtttt agaaaatagt
    15721 tattggcttc tgcatagtaa aattgtgcag attgatcatc taaaattcgt aaaggagcaa
    15781 taaattcaaa atgttctcct gcttgtaaag tatctatttt atttgtctgt gcaaaagacc
    15841 aaaaattaat aaagataata atgcccaata aaatagttcg catgtacaaa tatagattat
    15901 aattttttat tcttaaaggt tttacattat agatttgttt cttgttcatc aatccaaaag
    15961 acttgctctg taattaaatc acaaaataat tttcctttat ttgttaaagt ataggttgtt
    16021 ttattaaggt ttacccaatt gttgtctata aatggtttga tttcgaattc aaaatattta
    16081 cgcaactcag taccaaattt agtttctata tattgactgt ccacacccca aatagttcga
    16141 agatttgtca aagtatattc attataatgg tcaatattat ttagatgttc gcaactttgt
    16201 gccagttcgt tttgtaataa ttgttttata tataaattat tattactgat attccaaaat
    16261 ctattttgac cattatagct gtgtgcagat ggaccaatac ctaaataagg tttatttttc
    16321 caatagttag tattgtgaac agcatatttt ttgtctttac aaaaattaga aatttcatat
    16381 tgctcgtatc cattttgtgc aattgtatct tgtaaaatag taaattgctc cgcaattttt
    16441 tcttcatcta tggcttctac ttttttagtt tgtatctgat gatgtaaaat cgttttttct
    16501 tctacagtca aagcatagca agaaaaatgt tttatattta gttggattaa agtgtcgatg
    16561 ttttttttcc aatcagcatt acttaaagtt ggcgtaccat atatcaaatc acacgtgata
    16621 tcaaaaccaa tttgttgagc atcgagaata cattgtttgg cttgtgtggc attatgaact
    16681 ctttgcataa attttaaatc tgtataatta aatgattgaa tgccgattga aagtctattg
    16741 ataggtgtgt attgttttaa atcttgaaga tattccagcg ttaaatcatc aggatttgcc
    16801 tctaaagtga tttctgcctg ctcattaatt tcataattat catatatcgt tttgagtaat
    16861 tttacaatat aattcaaatc aataaaactt ggtgtaccac caccaaaata aatactatct
    16921 aattttgtgt tttttgaaaa aaaatgtttt tgcaaagcaa tttcattttc cattgcttga
    16981 atcattttag cactcgaatc tgtttgtgtc gaaaaatgaa aattacagta actacaagcg
    17041 tgtttacaat acggaatatg aatataaata cctgccattt tgcctcaaaa ttaaacaaat
    17101 actttattat taatgtataa gaccattgca aaagtcaatt tacaaaaata ttcaaggcac
    17161 taaattattt ttaaaccgca gttatcacat tgataatgag gattaaaaat aaatgagtaa
    17221 cgaagaagat tgaaggaatt tggctggaga tttatgaata atgattacct tgcaacggtc
    17281 ttaaatttgg acttgtataa atggataaat ttctaaaaat agggttttta ttgttattgg
    17341 gtttagtatt acaaaaccta atagctcaat caatttattt acaaagtcat tttatagaat
    17401 caaataaatc taatattaaa acgtttaaga agcaagctga tatattaact tatctggaag
    17461 agttagtgaa gaaaaaacaa aatgctggtt attggcaata ttcagtcgat agtttttact
    17521 gtacaaaaga tacctgtttt gctcagattt ttgaagggaa taaacatttc ttacaatcta
    17581 atattactgt aagtaagaaa acaacccaat tagatttttt gaaattgaaa gttcaattgg
    17641 ataaacaatt acaaaaatta gaaaattctg gctatccttt tgcacaaatc ttacaagatt
    17701 ctattcatct aaataaagat tccatttcaa tagcttataa aatcgatact ggacaattga
    17761 ttttagtgga tagttttatt aaccacgaaa atgctcgaat ttctaaggga tttattcaga
    17821 attatattgg aattaaatct ggaaagcctt acaatgaatc gaaaattgtg gagatagata
    17881 aattattggc aaaattgccc tataccgaat taaaacaacc atcagatatt tattttcacg
    17941 aagataaagc tgatattcat ttgtatttaa agaagcggaa agtcaataag tttgatttta
    18001 tagttggttt tttgcccaat agtagcaatt ctaataaatt gatgattaca ggcgaagcta
    18061 gaattcattt gcaaaatgca tttaaaagag gcgaggagtt gttttttgaa tggacacgat
    18121 tacatcccaa tacacaacga cttaatctga aatttcaata tcccttttta ttaaatacac
    18181 agttgggaat taatacaagt tttcaattag aaaagcgaga ttctacctca ttggatttga
    18241 atctaaattt aggcgttcct tattttttca aaaccaataa ctattttaaa ggtttttata
    18301 aatacgcaca aactataatt ttacaagcag atactaattt tgttaagagc ttcaaaagat
    18361 tacctggaaa cttagatgca acttataatc aatatggagt ggaattgtat tttgagactt
    18421 tagattattt attaaatcca cgaaaaggtg ttgagttgcg agtttcaacc tcagttggga
    18481 caaaaaaact gaagaccaat aatcagattt tagccattaa ttcaaatgat ggctttaatt
    18541 atccagcttt gtatgatagt atcaaaaaga aaagtctgaa agcagaatta ttttggaata
    18601 tcaattatta cttacctttt ggaaaacgaa gtaccataaa aatgagccaa aatggtggta
    18661 gtgtgtttaa taataatata ctaagaaatg aattgttaag aattggcggc aataaattac
    18721 tacgtggttt tgatgaagaa tcaatttatg caagtacata tattgtgaat acactagaat
    18781 atagattgct tttacaaaaa aatgcttata tgtctgtttt ttatgatttt gcgtatgttc
    18841 gagagcaatt tcaacaacaa ataaaaaata attttccgtt tggttttggt ataggtataa
    18901 attttgaatc aaaaatcggt atttttggac tctcgtatgc actcggacag cagaaaaatc
    18961 aacctatcag ttttcgcaac tctaaaatcc attttggtta tgttgcccta ttttaaacag
    19021 atagttttaa taatattgtg ttgttctata ttttattcat tttctgctga taaaaaagat
    19081 tacaatccaa tttgggtaga ttctatacta aaaaataaaa ctgattctgc tcagatgctg
    19141 caacttaagc aatccatttt tattgatggg caaaaggcaa caccaaccac agtgattatt
    19201 caaccgttac gagctcagca aaaagcctta gattatatgt atgcagcact gcttattttg
    19261 ttaattattg catttttata tatttttttt ggagattatt ttttagcagt cagagaaagt
    19321 ctagtaagta taaaacaata tcaggtttac ttagccgcca ataaatttga taactttttt
    19381 cttatagtgg cactttttct atttaaaatt attttaattg cttttgttgt aaactatttt
    19441 gtaaatatct atgtatcaaa atattatact atatttaatt acaaacaatt tacttatatt
    19501 ttatctgtaa ttgcaatttt ttttagtatc aaaatgatat tagaagtcat tgttttattt
    19561 attattgggc aaataaagac atacagactg ttttatttta ataaactatt ttttgatatt
    19621 gcatttgctt gtatggtagc cttaattatt gtcttgatgt attacaatac ttcaacagat
    19681 ataagagcgt ttttaattct ttttggagca atatatacac tttatattgt gtataaggga
    19741 tttaaaatta ctcagtttgc aaaaatttct aataaaatct attttattct gtatctttgc
    19801 acctttaaaa ttatgccatt aatgttagtt gtaaaatatt tttggcttaa tttaaaataa
    19861 aaattatggc gaagaaagtt acagaaaaaa aaatatcttc tactaagaag gtagctacac
    19921 ctaagaaggc tgctgtatct acattaaaaa agacagtaaa gccaactaaa tctaaagatg
    19981 aaattaaagc agtggagaaa aaaacagcga agactcctgt aaagaagact gcctccattt
    20041 caaaagaaaa gaaaactgta gcttcaacag aagctaaaaa gaagaatact gtaaagcaaa
    20101 ctataaaatc ttccgcttca aaatcttcag aaaaagcgaa gtcaattaag gtaagtgcta
    20161 aaattgagcc taaatctacc aagattacta aaaataaaat tgaagccgaa aagcctaaaa
    20221 gaaaatatgt taagaagtct gataaagaga ttacaaataa aattattgta cctgataata
    20281 aggtagtcat aaaagtttcg tcagataaac catattctgt aaaatcaatt ttaatttcct
    20341 taccgaaacc agaatcagga aaatcacctt atatagattt agccaatcaa ttcaagatta
    20401 aattagattg gcgaccattt actcaagttg aaggtattaa agccttagat tttagaactc
    20461 aaaaaatata tctagatcaa tttcaaaatg tcattcttac aagcaaaaca tcgattgagc
    20521 aatactttag aatgttggaa gatatgcgtt tgaaaatttc tccagaaaat aaatatttct
    20581 gtctaaatga agcaatagca ttatacctcc aaaaatatat caattataga aaacgtaaag
    20641 tcttttttgg tgatggaaaa ttaaataagt tgatagaagt cattcttaaa aataaagata
    20701 aagctggaga tgcaacctat ttgattccaa gttcagaaac taaagatagt ttattgaccg
    20761 atacattaaa atctaataaa attgaatata aagaagcaat tttatataaa accattgcga
    20821 gtgatttgtc tgatttaaaa aatgtaaaat atgatatgtt gatttttttc aatccatcag
    20881 caatagattc attgtattct aatttctcag attttgtaca agataagacc agattagcaa
    20941 tctttggaaa ttcaacaggt gagactgtaa tggcacataa attaagaatt gatgtctttg
    21001 caccaacacc tgaaaatcct tccttagttg atgctattaa aaaatatatt actgtaggaa
    21061 ataaaaagta agaattttta tttacctttc aattagaatt gacttttaaa ttctttagat
    21121 ttaattaaaa tgaaaataaa aagagtttta ttttatgtac ttttaataaa ttggcttttg
    21181 cctgtttatg ctcaaataaa accaatctct tcacatttta attttttaaa cgaattgtac
    21241 aatcctgcat tttatggaat tgatgagcaa tataaatttg cacttaatta cagaaaccaa
    21301 tgggcgagat tagacggtgc tccaagtact attaattttt tatctagttt ctatttacca
    21361 aaattaaaaa gtggtatcgg aatgaatata accaatgatc aaattggagc gtttaagaat
    21421 aaccaatttc agttaggtta taattatata atacccatta aagataaact aaaaattggt
    21481 attggagctc aagctggttt tgaatttagt aaactagatg gtacaaaatt aattacacca
    21541 aatggtgatt atagtagtgg aataaatcac aatgatgatt acctaaataa tcaaaaaata
    21601 aaatcctttc gaccttttct gaatataggc atttctatga atactaaata tttagatttg
    21661 ggtatagcat acttaaattc cataaataat gctaattcat ttgatgggat tttaaaggaa
    21721 ttaaagccta cttataaaag tgtattacaa acaaatctaa aatcaaaaat taagacgggt
    21781 gaaaattttt cgattgaacc tgctttgatg attaatacag attttgtgaa tatacaaact
    21841 gattttcagt tgatgtttaa ttatagaagt tttgtaggtt taggtacaaa tattagaggg
    21901 tacaataaaa atgctttcga atccgtttct actattatta aaataagtcc attgaaaaat
    21961 aaaatgttgg ggattattta tagttatgat ttcactttta aagaattagg aaatgtcaat
    22021 aaaggttcac acgaaattac attgtattat aatatgagga gtattggaat gaaaacaaag
    22081 ttgccaaagt tcataaataa ccctcgattt ctgtgaaaaa aatgctaaaa atatagattc
    22141 ttttaatttt tttttgattt aatatttttt tactattttt acaattactt tttatataaa
    22201 ataatgatta agatgaaaag agttacctac tcattgttaa tagtattcac actcgttttg
    22261 agtggttgta agggtggaaa aaccactgat gggcaactta taggctcaca ggatcgtcca
    22321 acttgggata ataacttgat gccatttgga atggtttatg ttccgtcggg tgtgtttcac
    22381 atgggacctt cagatcaaga tattaattat tcatttaata caaagatgaa acaaatctct
    22441 attgttgggt tttatatgga tgaaacagaa atcacaaaca atgaataccg tcaatttgtg
    22501 gaatgggtaa gagattcaat tgcccataaa atgttgggtg gagaccatgt tcaagaaggc
    22561 gaaaacggta gtcaagccat caattgggat atgcctattg attggtctgg tgctactggc
    22621 gaaacagaac aattagaatc attatattat acagaagccg atagaattaa tggtaaaaaa
    22681 gaattagatc cacgtaaatt aatatttgaa tatacttggt atagatggaa agaagctgct
    22741 gcaagagaaa ataaagataa acctcgtagt caatttatgg ataaaaaaca aatttctatt
    22801 tatcctgatg aattatgttg gattagagat ttctcttatt cttataatga accaatgaca
    22861 cgtgtatatt tcactcaccc agcttatgat gattatccag tggtgggtat tacttggcca
    22921 caagcaaatg gtttctgtgc ttggagaaca aaattgtgga atggttatag agaatcaaat
    22981 ggaatggctc cagttgaaga atttagatta ccaacagaat acgaatggga atatgcagca
    23041 agaggtggtc gaaatttatc tccatatcct tggggtggtc catatatcag aaataccaaa
    23101 ggatgtttat tggcaaactt taaacctggt agaggtaatt atcctgaaga tggtggctta
    23161 tatacagtaa atgcaaaatc ttattggcct aatgattatg gtttatataa tatgtcagga
    23221 aacgtttctg agtggacatc taccgcttat gatgatgcat ctgttatgtt tatccatgat
    23281 atgcaaccag attatagata tgatgctaaa gaagatgatg gctctgtaat gaaaagaaaa
    23341 gttattagag gtggttcttg gaaagatatt gctttctact tacaaactgg aactagaaat
    23401 tctgaatatc aagattcttc taaatcttat atcggattta gatgtttatt accgttctta
    23461 ggaagaggtc ttgaagattt taaataaaat aaataaatat tccaaataat taaacaaaca
    23521 attttaaact aatcaaaaaa aacaaattat gtcaagtttt cttcattcaa aatcttttgg
    23581 taagctcaag aatatgatat tcggtttggg agcttctgtg gtaattatgg gtgcattatt
    23641 taagttaatg cattggcctt tcgctagtga aatgctgatt gttggtatgt cggttgaagc
    23701 attcgtattt gcattctcgg ctttagcacc agcagaacct acttattatt gggagaagtt
    23761 atatccagga ttagatgatt atgatgcaaa aatttctgct gttgatgttt cagttgctgg
    23821 tggagctgga aataaaaaaa gtttagcagg cgaattagat aaaatgatgg aagctgcaaa
    23881 attggatcag aatttagtta atagatttgg agataactta aaaggattct ctgaaaatgt
    23941 tgcaaaaatg ggttcaatta ctgatgcaac tgttgcttca aatgaatttg ctaattctgc
    24001 aaaatctgct gcaacagcat tgacaggtgt acaaactgca tttactggag ctgctgaagg
    24061 tatgagtaaa ttagcttctg cgagtggtga tgcttctaaa tatcatgaac aagttcaaac
    24121 tgctgctaaa aatttatctg ctttgaatgc tgtttatgaa atggaattgc aagacactaa
    24181 caaccattta aaatcaatga ataaatttat tggcaattta gatgctgcta tgacgaattt
    24241 agaatcttct attgctgata ctgataaatt taaagcacaa atgggtggtt tagcttctaa
    24301 cttagaaaga ttaaataata tatatggtaa tatgctttct gcaatgaaag ctcaataatt
    24361 ttgctgagaa actgtattgt taaaaattaa aaattaaaaa gatatggcaa ttcctaagga
    24421 gaaccgtcag cagatgatca atatgatgta tctggtactg actgcccttt tggcattgaa
    24481 tgtttcagca gaaattttga atgcctttaa agtaatgaat aagagtttag aaaactcaaa
    24541 aactatcgca gattccaata tacaagctac atttcaagcg tttgctgata aaaaggttaa
    24601 agagcctgca aatatggatg tggtaaaata tgagcaacaa gctcagcaag ttaaaactga
    24661 aattgacaat ttaatgagtt atatttcagg cgtaaaacaa gagattgtta aaagagcaaa
    24721 tggtccagat accgaagatc cagatggtat tttatctaaa aaagatgact tagaaattgc
    24781 taatgcgtat attgttggtg aaggaaaggg tgaaggctat aaaattaagt ctaaattaga
    24841 ggaaactaat aataaactga tgtcgtttgt aagtgaagaa gataagaaaa agatgtctga
    24901 acttataggg ttaaatacaa atccagataa aaaagatggt gattgggtta gagaaagata
    24961 ttatcaaatg ccagctattg ctgtttattc aatgttgaca aaattagaag tagatgccaa
    25021 gaatgctgaa aatcaaatga tggcttattt atatagcaaa attggctcta tgaaagattt
    25081 aaagcctaaa gaaattgttt tagatgaatt ttctgctcaa attgcttcgc ctagtgctta
    25141 tgttttgcaa ggagaaccat ttaaagctta tgtatcttta gctgcgggta gttctgaaag
    25201 ttctgtgtct attacagtaa atggtcgttc tgttccagta aagaatggag ttggtgagtt
    25261 tactgtaggt acaggtagtg taggagaatt tccagtgaat ggtgtggtta cagttaaaaa
    25321 caatagaaca ggtgaagtta gtacttttaa aactcctgat tttaaatata ccgtagctcc
    25381 tccatttgct tcagttgctg ctgacaaaat gaatgtattt tacattggtg tggagaaccc
    25441 tattactgtt tctgctgcag gtgtttcacc acaaaaatta caagttggtc tttctggtgg
    25501 cggtagtctt tctggctctg gtggtaaata tactgttaaa gtaactcaac aaggaaaagt
    25561 tcaagtaaat gtttctgcta atggcaaaac gtatgctaat tctgaattcc gtgttaaaat
    25621 gatacctgat ccacaagctg aagtgggtaa taaaccaggt ggaagaatgc catctgctca
    25681 gtttaaagca cagaatggtt tgatagctgc attaaagaat tttgatttcg atgcaagatt
    25741 taatgtatta agtttccaat tattctacca acctagaatg gcagatcctg caattattcc
    25801 aaataatgga ccaacattta acggtgctgc tgaacaagct ataaataaag caaaaccagg
    25861 tgatgttttc tattttgaag agattaaagt tcaaggacca gatggttctg ttcgtaaaat
    25921 acctggtatt gcatttaaaa ttgactaata ttttttataa tatgagatat ttattatcat
    25981 ttttcttttt attgtctttt gctcctgcga ttttcgctgc agacgcaaca gaagtaaata
    26041 cagaaattca aaaattacct tggagtcaag agatttctgg aaagagaatt cctatggaag
    26101 acgtgtttat cagagaatct gatgtgatgt ggtataagaa cgtttggaga gtaatggatt
    26161 gtagagaaaa attaaatcta cattttaaat ggccaaaaag accttttatc ggtatcttat
    26221 tagatgctat taaatctggt gatgttccag tatattcagg tttagatgaa gatttttcta
    26281 cacctataac ggcttctgag gcacttagtg ttgctggtgg aagtacagat tctatcttag
    26341 ttcctgatgc tgcaaatcct gatgttatgg tttggaaagt aattacgaat gaagtaaact
    26401 gggataatgt taataaattc cgtatgaaag aagtatggta ttttgacaaa caacattcta
    26461 caatgaaagt tagaattatg ggaatttgtc ctttaattga taattatgac ccaacaacgg
    26521 gcgattttag agctgaagtt cctgtattct gggtgtactt cccatctatt agaaaaacat
    26581 tagttaatag tgaagctttc aacccatttc caaatggtgt tcgtttaaat tgggatcaag
    26641 tttttcaatt aagattattc tctagttata tctataaagt ggataacgtt ttagaccaac
    26701 gtataaaaga ctacaaatct ggtattgata ttttatatga atctgaagct caaaaacaac
    26761 aattgtttca atttgagcac gatttgtggg aatattaata cttagtattt acaatttaac
    26821 ggataacttt tagttatccg ttttttttct aattaaaatt atttttattc agtatttata
    26881 tttgtttttc aatttttatt ttggatattt atattaatat ccagtgtatg aaattagctt
    26941 ttattattaa tggaagtgtt aggaaattag atgctcttgt agaggcaatt cattctactt
    27001 ttagtactta tagtattgag gtttttattt ctgaatatgc acaacattat actgaattag
    27061 tacataaggc tttacataat caaatatcac atcttatttt tgttggtggt gatggtacaa
    27121 ttaatgaagg tatcaatgct tttattaatt tttacaaact ggatgataac tcttttgatt
    27181 gggtagctat tgctgctatt aaattagctg tctatcctgc tggtagtggt aatgattttg
    27241 taaaatcctt gtataaattt aagattgatt ctttgtttgc acttaaatca ttgattgata
    27301 gtgatagggt tcaattggta gatgttggat tggtgaatta tatggataaa agtaaactgc
    27361 caagttctcg tttttttatc aatattactg atgttggggt tggtggagat accgtccaac
    27421 gcaaagagaa tgttcctaaa tggctaggtg caaattttag ttatatgtgg gctatcacga
    27481 cttctattac acaatacaaa cctattgcca tgactgctgt ttatgatggt tataattgga
    27541 ctgggaaagc tatgagtctt gtggttgcaa atggtcggtt ctttggaaat gggctttgtg
    27601 tagctcctga tgctcattta gatagtggta tttttgaatt gacgattgtg ggagacatta
    27661 gtttggtaga gtatttaaaa aatttaggga agataaaaaa tggcataaaa attatacatc
    27721 ctgaagttca ttatttacaa gctaaagaaa ttgatatttc atcttcaaat agtgatttaa
    27781 tgaccattga tatggatggt gaatttattg gttatgcccc actcaatctt aattgtttat
    27841 ccaaaaaact tacatttttg tcagattatt agttaagttt ctagtattac cgagaaacac
    27901 gatttttaga gccaagaatc aagacttttt tgagtagaaa gtattgagta ttaagtattg
    27961 agaaattcat taatttatta tttcattact acattactac attaaaaaaa tcctgtccat
    28021 catttaatcc taaaaatcaa ggttcagaca attgccgagt attgagaaat tcattatttc
    28081 atcatttcat catttcatta ttacactaat cactaatcac taatcactaa aataaatcct
    28141 acccatcatt taatcctaaa aatcaaggtt cagacaattg ccgagtattg agaaattnnn
    28201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncat tatttcatta ttacattatt
    28321 taattactac attatttcat tacttcattt ttttttattc ctaataattt gataacccat
    28381 taaatgttgt attagatttt gattagttat actttagagt atgcaatttg cggaagatat
    28441 tagacggata gagaataaaa ttaacgaata ccaaaaggct ggtaagttga tgtttgcaag
    28501 ttctagtttt cagacacatt ctattccaat gttgcatatt atttctatta tagataatac
    28561 tattccaatt ttatttttga atacgggtta tctatttcca aaaacaatta cttataaaga
    28621 ccaaattgct gatttgtaca atttgaatgt cattgatgtt aaatctgcaa cacctagaaa
    28681 tatgcaaaaa gattataatg gtcgtttatt atttgcatct gatcccgatt tttgttgcca
    28741 tttgaataaa gtgcaaccaa tggaacccta tttgcaacaa tatgatattt ggataaatgg
    28801 cgttcgtgct gaccaaaatg cgaatagagc caatatgaaa gaggaacaag ctacgccaca
    28861 acgtgctatg cgttatcatc cgatgttgcc ttggacaaaa caaatgattt ggaaatacat
    28921 taaagaaaat aagattccgc aacatccttt agaaaatgaa ggttatacga gtattggttg
    28981 tgaaccatgt acaagaaaaa ttgacccaac tatgatgcaa gatgaacgta tggctcgatg
    29041 gtttgggttg aataaaacag agtgtggtct tcatacagaa ttaattaagt aaatataaaa
    29101 atgagagttt taatcacagg aggagcaggt tatttgggta ctgaaatcgt caagaaatta
    29161 gttcaaataa agacggttaa agaaattgta gtgtatgata atttaagtaa aggtaattac
    29221 aattttttct tgtgtacatg tattggaggt gataatattc gatttgttga aggagatatt
    29281 ttagatacta gaaagttaaa aaaggaatta gctaaagttg atgtagtaat tcatgctgct
    29341 gcaaaagtag ccacaccata taccgaccat gacccacatt tgtacgaaca agtaaaccat
    29401 tggggaaccg ctgagttgtc ttatgctatc gaagaaagta atgtacaaaa agtaattcat
    29461 attagtagtg tttctgttta tggatcatct gttaagagta aattaattga tgaaaataca
    29521 ccacttaatc ccaaaaccta ttacagtatt tctaaaatgc gtggcgagtc atttatggcg
    29581 cgattaggcg agaaaattcc tacacatatt attagaattg gtaatttata tggctttaac
    29641 caaagtatgc gttttgatgc agtgattaat agatttgctt ttgatgctaa ttttcataaa
    29701 aaaattacca ttcatggtaa tggaatgcag gaacgtgctt ttattaacgt tgataaagca
    29761 gcaaaaataa ttactaatct tttagttgat gaaaataaga aaagtagtct ttgtaattta
    29821 gtagatttta ataaaagtgt aatggatatt gctatgactt ttaaagaaat ttatcctgaa
    29881 ttggagatgc aatttattaa ccaacacctg aagttaagaa accttgcaat tggaactaac
    29941 tgctctatgt ttgatgaaat tagaacagga gaagttgata ttaaacaaga attgcaacat
    30001 attatcatgc agtttgcatt tcattagaga tttatttatt tttatcaata gatgaagata
    30061 aattatatag ttagttttga tgagtccaat actcattatg ccacaataaa tttaacgatt
    30121 tccaatattc aagaggatta cttggaattg aaaatgcctg tttggattcc tggaagctat
    30181 ttagtgcgtg agtttgctaa aaacattgat tttattttag atatagatac tcaaaaaaga
    30241 ctcaaaaaga agaataagaa tacttggttt ttggaggttc aagacagaaa aacgattcat
    30301 ttgcaatata aattgtactg ttttgaatgg agtgtaagaa ctaatcatat aaatcgggaa
    30361 catgcatttt taaatggagc tgcaactttt ttgtatattg aaaaatatca acaattgccc
    30421 attgaaattc agtttattcc aagttcaggt tggaaagaaa tatctacaaa tttggaatct
    30481 ataaatgaag atttctggca tagaatagca tcaaattttg atgaattgta tgatagcgtt
    30541 tttgaaattg ggaatcaaaa aatatattac tttgatgttc aacaaaccgc ttatactttg
    30601 gcaatttatg gtgaacatga tgctgaaata agtaaagtaa ttagcgattt acaggctata
    30661 attgaacaac aaatcatgat ttttgggcag catccttgtc gtcaatatct gtttattatt
    30721 catcatagtg aaaatgcatt tggtggctta gaacattgtc attcatcagt aaatcatatt
    30781 cctagaaatt attttcataa tcaagtaaaa tatcaacaag caattagttt attggcacat
    30841 gagcattttc atttatggaa tgtaaaaaga attaaaccgt ctgaatttat tccttttgat
    30901 tatgagcatg aaaattatac ggaaatgctt tggttttttg aaggcattac aagttattac
    30961 gatgatttaa cttgttatcg ggctggtgta ttttccaatc ttatttattt aaaagtgatt
    31021 gaagatttac tcgatattgt agaaaataat gctggaaatg attgccaaac tcttgcagaa
    31081 tctagttttg atacttggat aaaatattat agacccaatg aaaatacggg caatacacaa
    31141 gtgagttatt atcgtaaagg agctttggtg gttatgctca tggatattat tattcagtat
    31201 tattctggag gtaaatttag tttagataat ttgatgcatt cagcttttaa agatacatta
    31261 gtttctaatt actcaggaat tactaagccc tatatcattg aaaaattaga acaaatttgc
    31321 ttgtatgatt ggaatacttt ttacgaaaat tgtattgaaa gtactcaacc attagcgata
    31381 agtgaaatct ttaaattgag taattatcaa ttatcaattt ctgataaaaa ttctaaaaca
    31441 attggagtga aattgaagaa agtaggagaa caatttgcca taagttctat ggataaaaac
    31501 tattttaaat ttaatggaca attacaagta gatgatattg tcttgaaaat agataatcaa
    31561 gaactcatca ataactttga tgaaattata gagcaaaaag ctataggtaa aattttaaat
    31621 ttctacatac gaagagatgg tattgataag gaagttgagg taatgctgac tagaagtata
    31681 gacaagcgtt ttacagttag tcctaaataa aaaaaagcag gaactaaaat tcctgctttt
    31741 ttataaatta tttattgtat aattattagg caaatacttc ttctggaaat tttattctca
    31801 ttccatcaat ttctttagaa accctaatct gacaagttaa tctacaattt ccagtataac
    31861 aaggtaaggt ttctaaaata tctaattcac tatcagattt ttcatcaaga ttaagttcat
    31921 cttggtttaa aacttccact ctacaagtag cacacaaagc cattccacca caaattgctt
    31981 caattgggtg ttcttctcct ttaagtactt ccatcaaact catatctaca cctaaaggaa
    32041 tttcaagtgg tatgaattca aaattttctt gttgaatgta tattgtgata tgctccataa
    32101 atataaataa ctgttttttt aaaatgcatt aacgccgttt actgttgtat attttaaagt
    32161 tggtttttta ccattaataa tttctgctgc actttgacac atcaatgctg cttcatgaaa
    32221 accacaaaga attaatttca ttttattttc ataagtattg atatcgccaa tggcataaat
    32281 tccttttatg tttgtactat aatcttttgt atctactaag atagcatttt tttcaatatt
    32341 taaaccccaa tcagcaattg gtcctagctt cggacttaaa ccaaataaag ctagataatg
    32401 atcaacttca atgtcatgaa catctccatc tttatgcgtg atggttactt tattcaaatg
    32461 ctcatcgcca tgtaaatggg taatatttga atttaaaacc atttttaatt taccttcggc
    32521 ttctagttgt ttacatttat cgacactatc tggtgcccct ctaaacattt ctcctctgtg
    32581 aaccatagtc agttcagaag cgacattgga caaatacatc gtccagtcta atgcactatc
    32641 gccaccacca gcaacaacaa cttttttatt tctgaatttc tcagggtcaa taaccatgta
    32701 ttcaacacct ttattttcat aatcattgat gttttcaata gcaggttttc tcggctcaaa
    32761 aacgcccaat cctccagcaa tcgcaatgat tttagcatga atttttgtgc ctttatgtgt
    32821 tgttaagata aaagtttcgt cttcttgttt ttcaattcct tccactcttt cgcctaatgt
    32881 aaatgttggt gagaaaggtt ttgcttgttc cattaaattg tcaattaatt cttgagctaa
    32941 tactgatgga aagccaggaa tatcataaat tggtttttta gggtatattt ctgaaagttg
    33001 tccacctact tgtggtaaat aatccactaa atgacaacgt aacttgagta atcctgcttc
    33061 aaaaatagca aaaagtccaa caggaccagc tccaattata caaatatcag tttgaatcat
    33121 aagcttaaat taattttata tccaaaagta aataaagtga tattaaaaca atattattaa
    33181 atgagaaaaa ttatagtaat gatattattt tatgattgaa ttaacgatga gttgaaatta
    33241 cagagaataa atcttgcaat aaaagataat ttaatagttt ccttactcgc taagtgatta
    33301 tttcctacat caaaatgctt atttttgtaa tatgatatac aatttcagtc agatagaaga
    33361 gcaggcacgt caaaaatgga attatgatga taaaaataat tctatttata aagtagaaaa
    33421 taattcttca aaacctaaat attatgtatt ggatatgttt ccctatccaa gtggagcagg
    33481 tttgcatgtt ggtcatccat taggttatat tttttctgat attgtttcca gatataaaag
    33541 aatgaaaggc tttaatgtct tgcatccaat gggatttgat gcttttggat tgccagctga
    33601 acaatatgct attcaaactg gacaacatcc agcgattaca actcaaaaaa atattgaaac
    33661 ttatcgttct caattaagga aaattggttt ttgttatgat tggagtagag aaatttcgac
    33721 ttgtgaacct caatattatc aacatacaca gtggattttt ctacaattat tcaatcatta
    33781 ttacgataaa aaacaaacaa aagcacttcc aattacaaca cttattactg tttttgaaaa
    33841 tgaaggtaat acaaaaattc aagcagcttg tgaggataaa gtgataaagt ttagtgcagc
    33901 agaatggaaa agtttttctt ctacacaaca acaacaaatt ttaatggatt accgactagc
    33961 ctttcaaaaa gattcttttg tgaattggtg ccctgcattg ggtacagttt tggctaatga
    34021 tgaaattgtt aatggcgttt cagagcgtgg tgggcatcct gttgagaaaa agaaaatgag
    34081 ccaatggttt ttgagaataa cagcttatgc agaacgtttg ttgcaaggct tggatacctt
    34141 agattggtca gatgctatga aagaaatgca acgcaattgg atagggaaat ctaaaggtgc
    34201 gatggtagct tttcaattga aaaatcatca agataaatta gatgttttta cgacacgtgt
    34261 agatacaatt tttggtgttt catttatggt aattgctccc gaacatgact tagtgaaaca
    34321 aattactact gaggaatata agcaagcagt agatgaatat gtgaaaaaag ctagtgcaaa
    34381 atcagatatt gagcgacaag ctgaagtcaa agaaacaact ggacaattta ctggagcttt
    34441 cgtttttcat ccattcacaa atgctgaaat cccagttttt attgcagatt atgttttggc
    34501 aggatatgga actggtgcag taatggctgt gccttcaggc gaccaaagag attggctttt
    34561 tgctaagaaa tttgatttgc caattattgc tatttctgat gcacaaacta aattagaaaa
    34621 tgctgctgat gataccaaag agggcaaata tatcaattca gattttatca atggtttgac
    34681 ttataatgaa gcagttgata gactaattac taaattggaa gaacaaaaaa ttggatttgg
    34741 aaaaattcaa tatagaatgc gtgatgctgg ttatagtcgt caaagatatt ggggagagcc
    34801 atttccaatt tattatgatg agcaagggat tattcatacc gttgatgaag ataaattacc
    34861 attgactttg ccaagtgtag aaagttataa accaacagga acaggacaaa gtccgttggc
    34921 aactgcaagc aattgggtgc atcaatttgg agcaaatact cgaatggaaa ccgatacaat
    34981 gcctggttat gctggaagtt cgtggtatta tttacgttat atgcaaccag ataattcaaa
    35041 tgagattgta gattttaaag tgcaagatta ttggaaaaat gttgatttat atgttggtgg
    35101 aagtgaacac gcagtagggc atttattgta cagtcgtttt tggaataaat tcttgaaaga
    35161 tattggaaaa gtgagtgcag aagagccatt ccaaaagatg ataaatcaag gaatgataca
    35221 gggtgtaagt aagtttgttt ataggattag ctctaaattt caatttgata gttcaaaagc
    35281 aaagatatca gatactgatg cttttattct ttgggaaaaa tattttggta atttatttat
    35341 ctctaagaat attattgata aagtaaattt gttagatttc ttaaagataa aattacaaga
    35401 tggattacaa attttaaatg ataatggatt atatcttgaa tttgattcta tctctttatt
    35461 gaaactacat attgatgtaa aatatgtaaa aaacgatatt cttgatgttg aaagattaaa
    35521 gacagaagac cataattata aagacgcagt ttttataaca gaagaagatg gaaattatta
    35581 ttgcggaagt gaggtagaga aaatgtctaa atctaaattc aatgttgtga atcctgatga
    35641 aatgatagca caatatgggg ctgatgtttt taggatgttt gaaatgtttt taggcccgat
    35701 agatacggct aaaccttggg atactaatgg aatatctgga gtttctggat tttttagaaa
    35761 attctggaga ttgttttttg atgataatgg cattaaaaaa tatgcagata ctgaagcaac
    35821 tgctgatgaa cttaaaattt tgcataaatg catcaaaaaa atcaatcaag atattgagca
    35881 attaagtctt aatacttgtg tttctacttt catgattgct gttaatgatt taggaaaatt
    35941 accttctatt tcaaaatcta ccttagttga attgagtaaa ttattagcac catttgcacc
    36001 acatacctca gaagttttgt ggtctgaact tggtgagaat aattgtatcg ttaatgaaaa
    36061 ttatccagtt cataatgaag cttatttgat agaaagtagc tatacttatc caattcaagt
    36121 taatggaaaa cacagagcca atttagaaat tagcttgaca gcaacccaag aggaagtaga
    36181 gcaaattgta ttaggtgatg aacaaataac taaatattta gaaggtaaaa cacctaaaaa
    36241 aattatttta gttaaaggaa gaattgtgaa tattgtcatt tgattattaa gtgtaatctc
    36301 tcttttttca aaaaagatat ttaatttagt tatctaaact ttgaaaaatt atggatgaaa
    36361 tcaaatataa ttatcgtaaa tctatcttgc ggcattatgt aaattatacg cttacaaaag
    36421 atggattgct tatcacaagt cctgattata aagatagaat tataccttat catgaaataa
    36481 cttctttaag atttgagtat ttgctaaata atagatatga tagcaataga tatagttgta
    36541 ctatttatac aatagataat aaagaatatc aaatcctatc aacttcttat gcaggaatag
    36601 caaattttga aaatcaagct gaaccttatg ttgtttttgt aaaaggattg ttagcaatag
    36661 ttagaaaaat aaatccaaat tctaaaatct atttaggaca gtcaaaaata aaatattttg
    36721 gaaatatggt tttattaatt ttagtacttc tattgttgtt gagtgttatg tcctattttc
    36781 cagtttcagc ttcattctct attgtattca aacttattat gattgtcttt tttagttttt
    36841 atatggtgtt gtcatttact aaaaattatc caagagaact agaaattgga caagagattg
    36901 atgaaagcgt attacctaaa attaaatagt catattatta taaatcggaa accattaagt
    36961 tacaacctcg tatttctgca tagtcgattc tacctaaaac ttcaaaatgt gtgttggaat
    37021 aagcaatgcc catatcttgt gtttcaataa agctgcacga atataaattt cctaaatcta
    37081 taatattcaa aatacctctt tcactaaaat ttagaatttg ttttggattg tatatatcac
    37141 gacacaatat tttcatactt ggaatagtat ggtattgacc attttctttt gagtaagctt
    37201 gagacaacaa ttcagacata ccatattcag catgaattgc atcaacatta aaagcttttt
    37261 gtaaagtagc atgtacctct tgtttggtaa tttcttttct cctgcctttc attccaccag
    37321 tttccataat tatacagtga gataaatctt gaggataggc atttgcaaaa tccaataaag
    37381 caaaagttac acctaaaagt aaggtttttc tattgtcagt ttttgtttgt tgtaaaacat
    37441 gatttaattg ttcaaaatca gataaataaa acccagaatg tggtgaattg cttaatttga
    37501 ttaagtggtc aaacatatag accaatgaag aattttgtcg ttctaaataa tttggaagta
    37561 aagctatgat attataatct tgaatagcac catagaaatc ttgaaatcca tttttaaaaa
    37621 tgtgtgtata aagttctatg tctttaacat aatgtttgga attgatactt tgtgtagtgc
    37681 cactactttc aaaatagaaa tttggttgct ccgaaccaca ataaatatta tgtgttttga
    37741 aaaattgaat aggcaaaaaa ggaatctgct caatagattg aatagaatct atttttatat
    37801 ttgataagtc aacaaactca cgataaactt tgttatattt gtattgaaaa tagaagattt
    37861 ccagtgctaa gtcatcaaag tcatagttgt tggatttaat tttttcttct aactttgatg
    37921 cattaatcat gacaacaaaa ttaagtaata tatcattaaa attttttatt cttttagtct
    37981 gtttattttg ttttttagcg tgttttaagg aagcatttta tccaaatact ccaactgtta
    38041 agttcaataa atatgattta gagaataatg cttatcaata taatagctct aaaggttcgt
    38101 taattgtaga ttttactgat ggcgatggcg atttaggcgt agtttataaa gatacactga
    38161 taattgttgg cacggataca attaaaaaaa ttccaatttc aggtaaagta tattatacta
    38221 ttcctaaata tgatagtatt agaaattatg ttattgatga gtatgctttt cctgaaatta
    38281 gtagtgctta taatggttca aaaaatggta ttttagaaat taaatttact tctatttttg
    38341 atgaagtaaa gtattcaaaa attacaaata gctttcctgg aatacaaaga gatactatca
    38401 attttaaact ttggatgaag gatagagctg gtaatcaaag taatattata acaacaccag
    38461 attttataat taattataat taaacaaatg agaatattag ggcagattcc acatccaata
    38521 attaaaatta gtgttttagc attgaatgat aaatttatat tgaaattaga atatgattta
    38581 ttggagcaag tttataaaat tagaaattgt gatgaaatat ctaattttgg tacattggaa
    38641 gaattagtag atgaaaagtt tttagaatct tgtatcaaaa gattcaatga aatgaataaa
    38701 gatttatacg atacatttga acgaaataat attatgtgat tttctttttc tttcgtgaat
    38761 caatagtaaa ttataagcta caaaaaaagc ggactttata taaattaatg tccgcttttt
    38821 attataaaat ggtttgaatt attcgccttc ttgcatattt acaggcttaa aatctactct
    38881 tctgttgatt tgatgttgat tttcagtatg agcatctttt acagttgggt ctatttttcc
    38941 tagtggtaat cttttaaatc tatcttcttt gattccaaaa tttttaatca aataattaat
    39001 agcataattt actctatttt cagataattt ttggttatat tctaaagaac ctcttgtgtc
    39061 tgtatgacct tgtacttcaa ctattaaatc agatttcttt tgcataacag ctgcaatttt
    39121 ctttaattca gtagctgcag tcggcgtgat attatacttg tcataatcaa aatagataga
    39181 agttaatttc caagtatcct cttttttaat cgttgtttct ttaattgttt caatcgtatt
    39241 tgtttgaatt tcttttttct caataataat tttaggtgta tcaatgatgt taatacaatc
    39301 tacaatctta aatcttggat tagaaaatgg ctctgggtct aagtggtctg ggcaaccatc
    39361 accatcgcta tctaaagttc taccttttgt atccactttt gcaccttttg gtgtttcagg
    39421 ttcttcatca ataatatcaa aaacgccatc accatcagtg tccaataata ccattggttc
    39481 tggtttttct tgattttgat aagccataat tgcttccatt ggatttaccc attcaatatg
    39541 ctctgcttgt gaaccaattc taccaaactt ataggtaaaa ttaatattca tatataagta
    39601 tgcatcacga ttgatttttg aaagagaatt gatagtttgt ccagtcaatg tattgtagtt
    39661 attttggaaa ttaaaagcat ctaatttgtc agaattggag aaaacaaaag ttccttccaa
    39721 gcctaagtca aatgcttttg atactttaaa tttcattcct gttgtaaatg ggaaaatggt
    39781 ttcagtaaat ctgttggtat aacctctaag gtaagcagca ttttttatag ctaaatcatc
    39841 tccaatatta taaatattag attctccacg ccaaattcca atgcctattt tattatacaa
    39901 agcaaagatt cttggcttta ctggtttttg tttaatttgt gatttaatga atttattata
    39961 gccaaaagct aagcctaacc aatcgatata gaaatttaca gatgcttgat gaaatactga
    40021 tgttctgaaa tacacaggtt cttcaaatcc catttgtttc caatattgtc tttcttctgc
    40081 gaagccacct tgttcggcag ttttaccaga tattcttcct aaagtgtaat ctatattaat
    40141 accaaaagca gagttaaaca ttttagttaa tccaagtcca gctgcccact gaatttcact
    40201 cggacttttc gcacttctcg tccaatcata acttctgatg tcagtgaaag gagaagtcat
    40261 gcctccatga ataataaatg cccaatcttt taagtatttt ttggtctcta tattataaag
    40321 tgcctttgtg ttttttttgg tattttcttt cttaccagat tccgcaaata ttatgaaagt
    40381 tgaaaataca atacacaaga aaaaaataag ctttttcata ctaaaattat tttagtaacg
    40441 aaaatatgta aataatctta aattacaatg ggtttttgga ttaattcttt cttttaaaaa
    40501 aaggtttttt cttttctatt tcagaatctt gagtactgtc ttctctattt ttaacgttca
    40561 tagcaggtac taattcagaa tttcctttgg tttcaataag aaccccattt ttatatacct
    40621 ccaacttttc aaaagtaccg tttggtaaaa agtatgtcca ctttccatct cttcgattgc
    40681 agaattgctg tgttttgata ttctcccaag ttccatttgg atttttcata aactgacctt
    40741 caacctttgt acttccatct ttataatatt cagtgtactc gccacaaaga cagtctatat
    40801 attgaaatgc ttcatacttt attttatcat cttcatcaaa agttcttaat aggcatggtg
    40861 tacagccatc cattgcttgc caatattgac gatatttatc gtattttttt ttagtaattt
    40921 cttttccatt tacggtatat tttattccaa atggggtaga agtttcatat aaatttacgt
    40981 cgtaagtata aagcgtatct aatttttgat tttcgtctga tgttttatta ttataggaaa
    41041 aatcagattt atttggagtg tctattattg agttgtcttg agcataagaa acaataaata
    41101 aagccaataa agtcagtaga aaatagtttt tcataattac aaaagtataa actcttttat
    41161 acaataaaag gtttgtttaa ttaacaattg tgcaaatgaa atcaaaaact ataccaattc
    41221 aataattttc tgcatatcca aatcattcat acatttgaaa tgctttttag gacaatttgc
    41281 tcttccaaaa cgatgacaag gtctgcaact taaactttca ttttcgatat aaacagtgtt
    41341 gttgttgttt tgataaggat aatatcctaa gtttttatgt gtgccacccc agacagaaat
    41401 aattcttttt tgaaacgcag cagctatatg catcatgcca gtatctccag taattacaaa
    41461 ttttgctttt tttattattg atgcggaatt attaatattt agtttgccac aagcgttgta
    41521 gattttaaaa tcatcaatcg atgctaattg ttcggctctt tcataatcat ctgtaccacc
    41581 tactaaaatg attggcattt ttatttcttg acatagaact ttcaattttg acagcggaat
    41641 tgtttttgta aagtgtttgg ctccaacaac gataacacag taaccagcaa tatgcgtgaa
    41701 tggtaaattt gcaattaaaa tttcatcagc ttctggaata aaataatcta atccttttcc
    41761 atcatttatt acatttagtt tttttactgc tgtaaaatat ctgtcgataa tatgattatt
    41821 taatagattt attttaaaag aagtcaatag ccattgtttt atactttgtt tgttgaaagt
    41881 ataagtattc ttaatcttta acgcagattt tattt
//
LOCUS       DAOQCW010000006        38759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_360_length_38759_cov_6.306557, whole genome shotgun sequence.
ACCESSION   DAOQCW010000006 DAOQCW010000000
VERSION     DAOQCW010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 38759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38759
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_360_length_38759_cov_6.306557"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PLS16_01225"
     CDS             complement(<1..361)
                     /locus_tag="PLS16_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59727.1"
                     /translation="MDSNYLQDSNAENRKQDHIELAFKSQTAQSLSDKRFFYEPALAS
                     HPIEKNENIHFLEKNIQTPIWVSSMTGGTEKAYFINSNLAKACAKFKMGMGLGSCRSL
                     LDNNERIKDFDFRKIIGD"
     gene            complement(471..1157)
                     /locus_tag="PLS16_01230"
     CDS             complement(471..1157)
                     /locus_tag="PLS16_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HNJ59728.1"
                     /translation="MNSGMLYILMFVIMGVSWWVSYRLKSKFKKYSEIPIGLTGKEVA
                     EMMLQQYGITDVTVTSVPGALTDHYNPLNKTVNLSDWVYAQSNVAAAAVAAHECGHAV
                     QHATAYNWLTMRSKMVPMLEISSKYMQWIILAGLGVLAVTNNPYVLVVGVALFALTTL
                     FSFITLPVEFDASARAIHWIDQANIMRADQLDGAKDALKWAAMTYVVAALSSLVTLLY
                     YISLILRRRD"
     gene            complement(1276..3057)
                     /locus_tag="PLS16_01235"
     CDS             complement(1276..3057)
                     /locus_tag="PLS16_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59729.1"
                     /translation="MLFFAVQLYAQQGNYNHYLARQFVENKEFEKAAEYYADLFNQAD
                     GLQYYKEYYAVLNKIGNKENIEKLIKAAYKKSYNNPIYLIDLGIFYQQQNNRTEAEKI
                     YTKIIKDLPKNKFDILGIANYFEQSNLFEEAKKTYLKGKEILKSPYEFNLQIANLYLY
                     QDNISGMVQAYLDEAPSQADNLEIIENGLLKAINKNDKIKDEIEKSLYQRIGKDKTML
                     VYQDLLVWLYMQINDFEGAIIQAKSLDILRNENGNNIFVIANAALQQKDYNAAIKGFQ
                     YIVQKGNNNPWYYAASLSLINAQKEKITNQTNYTQTDLLKLKVDYLDFINSNADNQMV
                     YNATIDLASLEALYLHNTQDAIELLEELVKNPRLQATTIATAKLNLGDYYIMENNPWD
                     ARLLYTQVEKDQKGTALGEEAKFRNARLSYFKGDFEWAQTQLKIIKANTTELISNDAI
                     DLSVFILDNLNTDDTDEILLAFSRANLLEFQNKLDAAKDSFALLLQHASNSSLIDDIY
                     FEQYKIAKKQQKYSEALEYLLKIEKQYPTDILADDAIFYAAELYQNFLNDNLKAQNYY
                     EKIILEYKDSTFVIEARKRYRLLRGDI"
     gene            complement(3145..5352)
                     /gene="recQ"
                     /locus_tag="PLS16_01240"
     CDS             complement(3145..5352)
                     /gene="recQ"
                     /locus_tag="PLS16_01240"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HNJ59730.1"
                     /translation="MQTTFNLEQELKNYFGFDKFKGNQKKIIESILSKKDTMVIKPTG
                     GGKSLCYQLPAVVSEGTAIIVSPLIALMKNQVDLIRGYSNVDNIAHFLNSQLNKTQIN
                     TVKKDITSGITKLLYVAPESLIKQENIDFLKTISISFVAIDEAHCISEWGHDFRPEYR
                     KIRQMLNEIDKNIPVMALTATATPKVQADILKTLGMKDPEVFISSFLRENLYYEIKLK
                     QNKQDTVKDIIKFIKKQNGSSGIIYCLSRNTTEEIAEILAVNGINAVAYHAGLDANTR
                     SERQDQFLMEDLQVIVATIAFGMGIDKPDVRFVIHFDIPKSIENYYQETGRAGRDGLQ
                     GDCVAYFSPKEVHKFEKFILSNKEKSVTEKEIAVQQLAEVETFAQSAECRKKIVLHYF
                     GEETVNCGKCDNCIKPKEPIEIKNEILLALQALLETNEQTTSVNLVNILVGRKSAEIT
                     TKNHDKLKIFGKGSDKDMLFWSSVVSKAILENLLRKEIEEFGILKLTEKGKKFLKKQE
                     SIKGVLNHKFEEDEDDEDIITSTQKNTNVLEPLLLDVLKDIRKKVAKQHKVPTDIVFQ
                     EASLIEMATFFPITTEELSNIFGVNKGKAERYGKDFLEAIKKYIEENGIERYSDFSTI
                     KTADKNSNTKINIIKAIDKRMPLEHIASTYNMNMESLIIELETIINSGTKVNIDYYLN
                     EKIDEDIREEIFDYFREAETTDLKIAYKILKEDDIEYDEIQLMRIKFISDLAN"
     gene            5523..6605
                     /locus_tag="PLS16_01245"
     CDS             5523..6605
                     /locus_tag="PLS16_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HNJ59731.1"
                     /translation="MNSNTYVAIMAGGIGSRFWPASRTSTPKQFLDILGTGKSLIQGT
                     YERFLKICPKENIFILTHSDYIDLIKEQLPSITNNQILAEPARKNTAPCIAYAAFKIK
                     KINPNANIIVAPSDHIILNEDEFVRVSNLALDFVQKEKALVTLGIRPTRPDTGYGYIQ
                     YIEKEIQAEIHKVKTFTEKPNKEVAETFIESGDFLWNAGIFIWNVNSIIDAFEHLLIE
                     VYDAFQETESQLCTENENDAIQRAFIISPSISIDYGILEKADNVYVVPASFGWSDLGT
                     WASLYSEKEKDFMNNAVSSDNIIVYESYNNIIQADKDKLVVVSGLENYIIVDTDDVLL
                     ICKKEEEQRIKEFTYDIKLKKGESYL"
     gene            complement(6616..7803)
                     /locus_tag="PLS16_01250"
     CDS             complement(6616..7803)
                     /locus_tag="PLS16_01250"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNJ59732.1"
                     /translation="MYRKLKADFVFPISTAPVENGVIILNTNNEVVKIDEDKNYDDSE
                     LEIYEGFLVPGFVNAHCHLELSHLKNVAQTGTGLIDFIGQVIKNRNHSQEIIQAKIIA
                     AENEMLQNGIVAVGDISNAVDTFEQKQKGNLHYYTFVEYFDMFQNINTLPTIEQYDAV
                     YQQLAESETNKKSKVPHAPYSVSPKLFDYLIQNFDNNTDKTISIHNQETLSEDALFFN
                     KSGKFVDFYKNLNYTLEPIPKHLRSIHYALEFLKPHHRNLFIHNTCTTKEDILLAQKK
                     LGFQNTYWCTCPNANLYIENSLPNYQYFIDTKAQVCIGTDSLTSNWQLNILEEIKTIL
                     KYCSYLSFETVLNWATINGAKALGWQQKFGTLELGKTPGIVHINHTENKSITDKSQAR
                     RIV"
     gene            complement(7807..8400)
                     /locus_tag="PLS16_01255"
     CDS             complement(7807..8400)
                     /locus_tag="PLS16_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNJ59733.1"
                     /translation="METTTNIPVMIYTESTPNPNTLKFVINRPLYPNDFAEFESVSET
                     DKAPLAKALFSIEQITSVYISNNFVSVAKDDFIDWYELMPKIKSFLKNYIENGLPIIA
                     EDFIKPEKTATNTIFENDSDIEIKIKTALDKYVKPAVEMDGGNITFVSFINGILTVQL
                     QGSCNGCPSSTVTLKQGIENIMKKFIPEVQEVVAENG"
     gene            complement(8408..9430)
                     /gene="nadA"
                     /locus_tag="PLS16_01260"
     CDS             complement(8408..9430)
                     /gene="nadA"
                     /locus_tag="PLS16_01260"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNJ59734.1"
                     /translation="MENIASMDLKEKIAELPQRGFIDMDIDPTLDLFEEIQKLKKEKN
                     AVLLAHYYQNADIQDVADYIGDSLQLAQYAEKTDADLIVFAGVHFMAETAKILNPTKK
                     VVLPDLHAGCSLADSCLPGPFAEFKKQYPDHVVLSYINCSAEIKALSDYIVTSSSAET
                     IINTLPKDQKIIFAPDRNLGAYLSKKLGRDMVLWNGSCIVHEIFSLEKITKLQEKHPN
                     AKMIAHPECEEAVLNVADYIGSTSALLKYTSTDEATEFIVATEPGIIHKMMQASPNKT
                     FIPAPPNNACACNDCPFMKMNTLEKIYLCLKYEMPEIIIDENLRLAAKKSIDRMMEIS
                     AQAGIK"
     gene            complement(10036..10611)
                     /gene="nadD"
                     /locus_tag="PLS16_01265"
     CDS             complement(10036..10611)
                     /gene="nadD"
                     /locus_tag="PLS16_01265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HNJ59735.1"
                     /translation="MKIGLYFGSFNPIHIGHLLVAIHIREAANLDEIWFIVSPQNPFK
                     KNVDLAPENHRLEMVKLAIEPISNFKVLDIEFNLPKPSYTHLTLKELKKNYPKYDFKL
                     IIGADNIEKFHEWKETEWIQKNVEILVYNRNKNQASSVNNQDNYFKIQNLPLLDISST
                     EIRNRIKENKSILYFIPEKVENYIVDNQIYL"
     gene            complement(10608..11774)
                     /locus_tag="PLS16_01270"
     CDS             complement(10608..11774)
                     /locus_tag="PLS16_01270"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNJ59736.1"
                     /translation="MLKINETIYQNINIALLSTFVVGLFFSNAVLSISTLLFFAWVIT
                     NKFKEIYLKENLPLGFLLLFMVYLFGAFWKTNNILDSLQTIISKISLLGFAFIYSIKI
                     ISNQQKKILFNLILFFLIGISFYTIIQYFSDKNYWNNLYSKGQVLPTFMHHVKFSLLI
                     VLSINILTYTNIINNKFKNIIILYFIIYLHILAVKSGLLILYISIIIYIIQKIYYKKY
                     QYIIILLFPVIGYLFSTNLQNKINYLKYDIQQLHQENVLDYSDARRIVSFQIAWNIFK
                     ENKLLGVGLTNIKKETENKYNAIYKYFDASKMMYVHNSYLQILVSTGVVGFILFTFSI
                     IIILKEYWINNKLLFILNSLFLMVCFWDALTEQLVGLSIFILIQILGKQENLKQ"
     gene            complement(11793..12302)
                     /locus_tag="PLS16_01275"
     CDS             complement(11793..12302)
                     /locus_tag="PLS16_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59737.1"
                     /translation="MLKNFLFIFLIFSLTNSFTQESKGAIGLRFGYGWGLSGKYFLDR
                     NGNALEFTVKQGVHGTLYNTSVLNFGTAYQKHFEIDRRGKWFVYVGGGGNIGFGKNAP
                     KQAIISGGLAPVVGLDFWTQNLIVPFNLSFDYAPTFYYDRITKIKKNDFSFSYLNFNV
                     AVRVGLGRL"
     gene            complement(12363..13112)
                     /locus_tag="PLS16_01280"
     CDS             complement(12363..13112)
                     /locus_tag="PLS16_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNJ59738.1"
                     /translation="MSAPFLIHLPQFEGPFDLLLFFIERDELDIEDIPIFKITKDFLN
                     YIHHLERLNIEVASEFMLVAASLMRIKAKMLLPRKELDEQGNEIDPRQELIQQLVEYK
                     KIKEVVEEIKDLEAQRSLRHTRGNQKAEVEDLIKILADDVELETVSLIKLMRAYQRAL
                     DKYKTRTEKITHKIVQYEYTIEEERNKLASLIKEKKKSSFEEILTACENKMHAVFIFL
                     GMLELIQQQVIFLVLGEGNNNFWLSSTRIDD"
     gene            13177..14442
                     /locus_tag="PLS16_01285"
     CDS             13177..14442
                     /locus_tag="PLS16_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59739.1"
                     /translation="MQIVIYTTEVTNRLKYVLDFIFVQYFGIDYFLETDIVVFKKQTL
                     IANFICINYSKEQINNCINISCEKLLFQTNIIPQKIFIALIDEMPVIFPSEFLDSSFQ
                     FDIFAAIFYLITRYEEYLPHEQDEHGRYCSHNSILAKPEFSFKPIVEIWLAYFKSILL
                     NIQPGLEFKRHKFTKIFTFDIDNAFQYKHRNWFKHPPNIFITEVRNVLFGKQNDKYNT
                     FDELFQFCNQLSHKPYFFFLLNDSSRRNSNVSPKANALHHIIKNCTEFYIGIHSSYHF
                     SQTFFIREIQLLSKIVNYPIVFSRQHFLKISFPDYFRTLVEYGITKDFSLSYPNISGC
                     RAGTTQAFFFFDIINDKQTSLLIQPFIFMDATYQYYIHDDNYTIQHKVEKLLDEIQRI
                     NGNFVSLFHNDLLHDSLPFKNIMKNIQEY"
     gene            14452..15486
                     /locus_tag="PLS16_01290"
     CDS             14452..15486
                     /locus_tag="PLS16_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNJ59740.1"
                     /translation="MFNYIYRGVWILFIFLFSFVQLYSQDYKFAQYYNSPLNLNPALT
                     GKINSLYRLTANYRMQYFTVQAPSPYTTISGSADFGLFRDKLNNDIFGLGIVFGNDRQ
                     TILRTNKLDLSAAYHKSIGYKKQHYFSAGLQIGFIQRTIDLNNLYFGAQFDEDRFDLT
                     KPNLENFNSNKFIKPDFNLGLFWTSSLNKDKIGIFAGVSAFNMFLPNQSFLSSNYERS
                     MRFVVYSGASFQIKKFMMIIPNTLFALQAKSIDWSLGSNFAFNISEKRYAFKSSVLFG
                     VFYNGNGLISTNVGIQHQGFMAMLGYDADLVKDINNAVKSVGAMEISLIYTGNPIKSK
                     IYQPLFCPKF"
     gene            16034..17896
                     /gene="mnmG"
                     /locus_tag="PLS16_01295"
     CDS             16034..17896
                     /gene="mnmG"
                     /locus_tag="PLS16_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNJ59741.1"
                     /translation="MFQTYDVIVVGAGHAGCEAAVAAAKMGSKVLLVTMNMQTIAQMS
                     CNPAMGGVAKGQILREIDALGGYSGIVSDKTMIQFRMLNLSKGPAMWSPRTQNDRMLF
                     HTTWREMLEQTPNIDFWQDMANGLIVENGRVCGVETSLGIKIKAKAVVLTNGTFLNGI
                     IHIGEKQIGGGRAGEKAAKGITENLVRLGFESGRMKTGTPPRIDGRSLDYSKMDIQNG
                     DEAKYGFSYKNISRPKEQRCCWITYTNENVHELLKEGFDKSPMFQGRIQGIGPRYCPS
                     IEDKINRFADKDRHQIFVEPEGWNTVEIYVNGFSTSLPEDVQYKAIRQIPGFENCKMF
                     RPGYAIEYDYFPPTQLHVSLETKAIKNLFFAGQINGTTGYEEAAAQGTMAGINAHLAI
                     NDKEPLILKRSEAYIGVLIDDLVNKGTEEPYRMFTSRAEFRILLRQDNADIRLTELSY
                     KLGFAEEERMKRVEEKINATNEIRNFAKNYSVEGKDIDTYLEDVGTTKLYQKTKLDKI
                     ITRPQINLLPLVEYVPSIKQHLEKYEEEFLEQAEINIKYEGYIEKEFEQVKKLEKLED
                     YKLKPDFDYNQIKSLSIEGRQKLNKIKPLTIGQASRISGVSPADISVLMVYLGR"
     gene            17925..19682
                     /locus_tag="PLS16_01300"
     CDS             17925..19682
                     /locus_tag="PLS16_01300"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNJ59742.1"
                     /translation="MIINKGAFINGISRFLHTHLRSFLYLFILSFLMNACAVISAPTG
                     GEKDKIAPKAIKINPQNETVNFNAKEINIDFDEFIQLQNPQNILITPDINPKPVFTSN
                     RKSLNIKFKTTLDSNTTYSIFFGSELKDYTEGNPTDNFTYVFSTGEYIDSLKIKGSIQ
                     NYEDKLPQNTYAILYKDLGDSVFTTKRPNYISRIQTDGTFEINHLKEGQYQIFALADN
                     NSNYYYDLPTEAIGFLKENIQVDSNSQNIILPLFIPEVAQIRIIEKDKAIQNGIFNIT
                     WNKTLSPKTDQFEIVSLPETKALAFFEDKTMRIYFPNLQTDTGHIQLFVKHNGKIVDS
                     IETKLTSKKTSNIFFDSIQYKSIKQLQSNPLKLVASKISIADIDTNKIYIIDTSENKI
                     PFSITQEEDFKTYKLTGNFLPQNYTIYILDSCFLDLAGNFNTAQQISLSVLDNKKGGN
                     LLINIELDTIYPNLIFILKDNQGKVLKNEFLSNSQTVKINIGLIQAGEYKVELIRDDN
                     QNEIWNSGSFINKTLPEKIYYHPTPIIVKENWDAEETIQPKFDEVKKSILNLQNKKQE
                     LNQQINNNSSIPFQNRKLK"
     gene            19830..20234
                     /locus_tag="PLS16_01305"
     CDS             19830..20234
                     /locus_tag="PLS16_01305"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ59743.1"
                     /translation="MKEITQLIFDSNLDNIAKVEQAIEDIKQNYTITDTIYGNVMIAS
                     IEAVTNAIEHGNSFDNTKNVFFKAYICNQSLKISVEDEGCGFDVDTLPDPTTIENKGN
                     PDGRGVFLMRHLADDIKFENNGTRVELFFRLN"
     gene            20474..21331
                     /locus_tag="PLS16_01310"
     CDS             20474..21331
                     /locus_tag="PLS16_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ59744.1"
                     /translation="MSKIKEANVLVTGGGNGIGKQMGLKCLQDGCANLVIWDINKDNL
                     DKTKKDFSNRGFKNVHTFLVDVSDIDDVEKAGTEVLLEIGNIDILINNAGIVAGTKSF
                     WDYTAKDIDRTVDINIKGVMYVTRVFIKEMLRQKKGHVVNIVSASAYLGLPKGSVYAS
                     SKWGAMGWSESLRLELEREEGDLHVTAVCPSYIDTGMFKGVKAPLLFPLLEPENTAER
                     IIKAVKSNQVHLLLPDTLNLVPVLKGIFSPKIFDVVADWLGVYTSMNSFEGRAEKERI
                     PESKKQKSK"
     gene            21358..22746
                     /locus_tag="PLS16_01315"
     CDS             21358..22746
                     /locus_tag="PLS16_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HNJ59745.1"
                     /translation="MATKQARKTGQIQQIISEQQAFFETDKTKDVNFRIAALKKLKAS
                     IQKNEGKIYEALHNDFSKSGFETYTSEIGTLYEEIGFLLKHIKNWAMPQAVKDTVANF
                     PSKNYIYPEPYGVCLVIGAWNYPLQLTLSPVIGAIAAGNTVIVKPPRASMHTYQITQQ
                     ILSVFEEDYICVLPEDSNNDEMLQCRYDYIFFTGGVEVGRKVARAAAEHLTPTTLELG
                     GKSPCIVDASADVDISAKRIVWGKFMNGGQTCIAPDYLLLNSKVKDSFYQSFKKYVKE
                     FYGENPFFSADFPRIINDRHFSRLNKMLVDGKIIVGGETDKESKYIAPTLIEIKDLNH
                     PLMEDEIFGPILPVLEVDSVDESIAIIKQFEKPLALYLFSKNYEEQQKVLKKISFGGG
                     CINDTIAHFVNKELPFGGVGNSGIGAYHGKYSFDTFSHHKGIVHKVTWPDVPLRYPPY
                     KGKTPIIKQILK"
     gene            22865..23509
                     /locus_tag="PLS16_01320"
     CDS             22865..23509
                     /locus_tag="PLS16_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNJ59746.1"
                     /translation="MEGLNENKEINLELEDQVFCKAKDLFMSIGIRSTTMDDIAKDLG
                     ISKKTLYKVVENKGDLVTQCVIHDANARRLEVDAIVKEYSNPIEQMLQLGIHILNSIK
                     QHKISIIHDLMKFYPESWRIITEHKETYVKEIIKQNLKKGIQSGAYRKDIKIDIYANF
                     FIAGTDICIDTRIFPESEFEFPDIFREYLLAHLRGIVNESSFTELNQLIEKVKF"
     assembly_gap    23651..23750
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <23751..24689
                     /locus_tag="PLS16_01325"
     CDS             <23751..24689
                     /locus_tag="PLS16_01325"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNJ59747.1"
                     /translation="NEELNKLKLQKTEEDVIYNTVAAYYQLLIINENEKLLNESEVRI
                     NKTLPIVQLQYDKGVARKIDVDKIKVNLNNILANKEILRINKSMAMNNLKYTIGMPLE
                     TEIVIDPKFENEVSTDILNNSDNVKDKVELKLLNTSLILNEINYRRQKAAYYPTIAFA
                     AKYAGNALGDKLADSFKKWTQLAVVGVKITVPIFDGLRNSSALKQMSLNNENIKYNIK
                     QTEDGLKLQLQNAKTGISNAVQNYTINKENLELAKSIFDVTTLQYQKGVVSYTEVLSS
                     EFSYKEAEVNYLQSLVKYLNYKLELDKANNNIQSYK"
     gene            24709..25767
                     /locus_tag="PLS16_01330"
     CDS             24709..25767
                     /locus_tag="PLS16_01330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNJ59748.1"
                     /translation="MKTKIFLIIAGLLIVGGAAYKLAKNKKIIDDNAKPIDRSGVPIA
                     VTAFEAAYFPVSSDFALPGVLDQNNTGNVNAINPGKIVSLNIEVGQQVTKGQIIGKVD
                     SKLKEIALQQAELTIKKLETDAVRTKDLIEGNAAPATSILDLDYNIENTKIQKDNIKA
                     QIANDNIYAPISGMIIQKNSNAGEFINPGTPIATIMDISILKAVVYVSENNVYELKIG
                     QQAQVLSSIYPDKIVTGTIKYIAPRGDENHNYKVEVHIPNSGYKAGTYVSVKFAFKKP
                     AEALLIPKIALVEGVKNPYVYVVSGNNVIVKKIEVGEEVGENIVVRNGLVAGDKVVTS
                     GQINLSEKSKIQIVKSNQ"
     gene            25774..28911
                     /locus_tag="PLS16_01335"
     CDS             25774..28911
                     /locus_tag="PLS16_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNJ59749.1"
                     /translation="MSITEVAIKRPLLISVLFLVLILAGFLGYKELNYNLLPKFEAPV
                     LSIQTVYRGASAEEVETNVTKKIEDAVSSIEGLDIISSTSMENASIVILQLKQNTVVN
                     DAQQEAQRKLNNVKNELPDGVDDPVISKFSSSDLPVLKLTLFADNLGESQLYDFADVT
                     IKPIILNLDGVGQAKLIGGSEREISVMLDNEKLRGYNLSSAQVSQAISMGNMSFPVGS
                     IENNSNRFNLRLDAKLVSVEQLRNLIVRENKNGSKILLKDIATVTDGTIEPVTINRIN
                     GRVGLGMEINKQTDANAVEVAKIVKEKLDTLVNQYKSTGLNYEIAVDQSKYTLEAANA
                     VQHDLILAIFIVALVMLFFLHSLKSSFFVLVALPSAMIPTFMMMYLMGFSLNLMTLLA
                     LSLVVGVLVDDSIVILENIYRHMEMGKNRVKASLEGRSEIAFTALAITLVDVVVFVPL
                     SLAGGLIGNILREFALVVVFSTLMSLLVSFTLTPLLASRFGSVAHLDENTLWGKLNLK
                     FESIIDALINQYAKALDWGLSHKRYVVLGVICLFAGAFSLAGFGFIGSSFAGSGDRGE
                     FNLIVELDPQSTLIQTNKVVKKIEDIILEYPEVDKVFTKVGAVGTQMGESIATPNQAD
                     ITTTLIDKTKRKITTEQLSIQLRNRFDSIPGIKFSIKPTTVTGNNTPQIQIVVMNTNH
                     DSLQKATQMIFDFVKQTPGTDYVELSTKKSKTEVEIVLDRDKIAKMGLSIPMVGAAVQ
                     LAFRGNDQSKFKDKGEEYTINILYDKEDRKSITNIENTILQTPSGAIIRVGDIADVKE
                     ISAPEKLERYNRLSSLQVLASTVGRPTGSVMADIKLDIEKANFPIGTSVDYLGEEKNQ
                     ADSFGSLGIAMGIGILLVYLIMVALYESMLYPFVVLFSIPVALIGAILALALTMESLS
                     IFGIIGFIMLMGLVAKNGILIVDFTNHIKAQGYGLKESLIEAGRERLRPILMTTLAMV
                     FGMLPIALSNGPGAEFKKSMAWVIIGGLTSSLLLTLFVVPTVYYILETLKNKIQGRKG
                     ENAPKKKTISEMISE"
     gene            28934..29467
                     /locus_tag="PLS16_01340"
     CDS             28934..29467
                     /locus_tag="PLS16_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HNJ59750.1"
                     /translation="MEFSYLKALHIIFVVNWFAGLFYMPRLFIYTKEASEKKEPARSI
                     LLEQNLIMQQRLWNIITWPSAIITLLLATLILIQNPFFLQQPWLHTKLVFVGLLYGYH
                     IYCHIIYKQQQQHIFKLSSKQLRIWNELATILLFIIVFLVVVKSQMSILFAIIGVFSL
                     AVLLMAGIKFYDKIRKK"
     gene            29567..31240
                     /locus_tag="PLS16_01345"
     CDS             29567..31240
                     /locus_tag="PLS16_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59751.1"
                     /translation="MNLFYKNFYRYYWLISLFTLYLFFRNYQTFLDVSLLDESGYLLL
                     SRFNPLIYPGGYGALYILCYKILHQLISNSMQLHFFAIIFLTWLPSIIVFYFLFFRKV
                     PAFLCVFISWTLMLSPYIAAFDWWPRISHFAISFIFIFTIISFKYHFNKYKFLLFGIL
                     VCGILSYARPELMLSVYMLLGVLIAIFSYERIVQKKQSNVGFTKRNKILFSIMTFVFL
                     AFLLVWKNPLKNTGRAYFALGQHYTFNVLRWENKDMTYFIRWPEVFEKKFGKSRTFKQ
                     MYKYNPSETKRHVVENIKHYFEQTYRFLPELWMPSILFKISATIKYIVFFLIYLVCLF
                     YLGLNTYWQQLKQKVIDNFFYVLFFLIIATPSLFASFIIYPREHYFIMQLPLFLFLIY
                     LLIEPFFSLKVKPSLSKVTLVITIFIILILTPNISAYPRFNKFDRYEQPNYVPYIKYL
                     ESLKIDKKVNFLTMEFLQIYLPNNYAYATEFFTKKPFYDSLIVAKDINLIYLSPYMEN
                     EPRHQSDSTFKYFMNNYSRLGWNKVQLKDKTGYLLIKQELLGLHSLIIE"
     gene            31492..31776
                     /locus_tag="PLS16_01350"
     CDS             31492..31776
                     /locus_tag="PLS16_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59752.1"
                     /translation="MKDIFKILFIVCISTFYSSSYGQSCFKNNYSGFSNTTNYLDVDT
                     TNFIVTNGGSQLENYHLSSLKLAIEIGKIADTLGNEISCSSAGFHQNTPN"
     gene            31781..33379
                     /locus_tag="PLS16_01355"
     CDS             31781..33379
                     /locus_tag="PLS16_01355"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNJ59753.1"
                     /translation="MNKFTAYRVEYTPELSVVTGLDKIKFKLRALVIKPNNASNAPCV
                     LVTHGNDGVLRDWRGNLLYGVSDLLMKGYVVVIFENLSTQRRVPRSDIWGCISKTYKY
                     VSAPGTPICMSTMLFHIAYINCGGQQSGSRWKLYNFLTIDALAKYIAQNSSTYNVNPN
                     KLFTYGFSFGSGVAEGFLTQKTEFPSALLYNTSVNHTPIRQAGFNSTVGVNFTISSAD
                     ISKYTNLGGIPMSSPLMNADNNCGKVDVYTADDSTKRLLLIHGFADSATTGSIYFNVY
                     PRKQEILDSVNIKNHLITVCGEGHSIFNGFDNFISATGNIFKTTDRNYPFYSFVKGLT
                     ISNINTMKDSLNLNTTYKNFMDTIRGIDNQIFQLYKSGSQFFVNAINNTSYILCSTEN
                     SYDRKTYIGNDGIYGSTYNANDWLYKIYNASCELPSGERKAKEMNSIKEMGFNIPMLY
                     PNPSNGIFNIDIPVIFNIKELTLVIYNSNGQVVSSQIINKTYQTGEIIKEQFDISNQS
                     NGNYYIQIRSDTRILLSQIVILKI"
     gene            33422..34633
                     /locus_tag="PLS16_01360"
     CDS             33422..34633
                     /locus_tag="PLS16_01360"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNJ59754.1"
                     /translation="MQPYFQTNNTNITQFDSIRFVIAIFVIIVHHQFTNINIPFAFAY
                     MAMHIFFIMSAFLISRGLLLSKSKAQTFSLFFKDFYKKRILRIFPVYFAYLFFLIGIS
                     IALYLLKFGDILKIITELKKFGWMLFTFTYNYREFLLHIKNMPLIESFFFSHLWSLSM
                     EEQFYIVIPFVIYFMNRKQLVWLSVIAIIITPIFRVWYYENVLVNEEDYILRGLIYYR
                     ATFLQFDCFFYGILLATYNFKDFIKTYKIIFYLLLIVYLFVIIYNGFIISKEMNLSFF
                     SSIIRYDFMILNKQYLYHDVLINWLIFFMFLNCFYNPNFLSFLNFKFILNAGNKFTYS
                     SYVYQYLFILPSVGILYPLLTKVLGLTEVLAQLIALIVSSVGIYYLSSFSYKKMEIKF
                     LNLLKKEKHAK"
     gene            34623..35915
                     /locus_tag="PLS16_01365"
     CDS             34623..35915
                     /locus_tag="PLS16_01365"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNJ59755.1"
                     /translation="MQNSATQNIEIKKPSVQYFEVIDGLRGSSMLMTIMNHMFFLQIL
                     KIPVVFAHFGLHGFFILSAFLISSILYKEKEKFGVYKPYVKNFYIKRILRIFPVYFLY
                     LFAVLMLGLATKGTPLQAPLGIIYDLKHYGWMLLTFTFNYRELYSWLINESHLRCMFF
                     PHLWSISLEEQFYLVAPTLVFFFRKETIKKIAIASIIIYPFFRFFGYLYLLNDVKMIS
                     PFTVDGHDELSALYDFFYRSSLFQFDAFMYGILIPLVSYDNRKVLRWIVIISFFTLLA
                     SQLYNIIDLAHDSGRPILQVANHADVIMRNGQFGYINTLYNILGASLFYYLLKFPDSS
                     IQKPLRFSFFKNCGRIVYGIYVYHPIFIMITLVLYNLFFSMISKEVGDMFIVKLLADI
                     GAVAFCFTTTYYVCKLSFYKFEMPFLLLKAKRSEQKKI"
     gene            36098..37231
                     /locus_tag="PLS16_01370"
     CDS             36098..37231
                     /locus_tag="PLS16_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HNJ59756.1"
                     /translation="MDFERLTFSIHQTNDFLLKNAVKAVNVNITLRNWLIGFYIVEFE
                     QKGSDRAKYGTKLLENLAKNISIKGLSAPELSRCRQFYNTYPQILATVTQKFKTLVPT
                     HFIEDFVHKSILGIASQELDNNIISQSTTDQSQNEHLHQLLSSSSYSHLVELIKIEDS
                     TKRRFYELLILKTQPSVKELKRQIDTLTFERVGLSGNYEKSFDKIIKKITPQQTNDLV
                     KSHYFFEFLNIEQPHLIEESALEQALIQNLKQFITELGNGFCFEARQKRILIGDEYYF
                     IDLVFYHRILKCHILIELKTEIAKHEHIGQLKTYLNYYKKNIQEVNDNPPVGILLVTN
                     QNKALVEYAIADSDLDIFVSKYQLQLPDKVELETFINNELKNR"
     gene            37407..38651
                     /locus_tag="PLS16_01375"
     CDS             37407..38651
                     /locus_tag="PLS16_01375"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNJ59757.1"
                     /translation="MSEEQKVHKEQTTRSIGYIESIDGIRGFYVFPIIIMHLHLQHIK
                     MPAFIPHFTLHLFFILSAYLISSILYKQKEKSESFKLFAKDFYIKRALRILPLYWAYI
                     LFFFGVAVVLRTVAPALEQNFLGVIHDLKHYGWMLLTFTFNYRELFSWSQDLSHLRCH
                     VFPHLWSISIEEQFYLIIPTLIFFLNKKSIKIISIIVVCVYPLIRTFGYIYFRDVVEM
                     RTTYGDEWNALFNFFYRSSFFQFDAFFYGLLIPLIRIEKRYILYAGYFISLLLMILSQ
                     SYNAVLIADEQKISFWSAIHLPTVIYSNNQFMYMNTLYNIFGFFLIYIMINDPKSLLN
                     KFYNAKVFRPIGKVIYGMYVYHPVFALLTLALYYGFLVKVIPNILADIIAIAICTTAT
                     YYFAKLSYYKFEIYFLNLKSKK"
BASE COUNT        13463 a         5210 c         5810 g        14176 t
ORIGIN      
        1 catctccaat aatttttctg aaatcaaaat ctttgatacg ttcattatta tccaagagtg
       61 aacgacatga acctaaaccc atacccattt taaatttagc acatgctttg gctaagttgg
      121 aattgataaa ataggctttt tcagttccac cagtcatact agaaacccaa atcggtgttt
      181 gaatattttt ttctaaaaaa tgaatatttt catttttttc aatcggatgt gaagctaaag
      241 caggttcata aaaaaaacgt ttatccgaca aactttgggc agtctgagat ttaaaagcta
      301 attctatatg atcttgcttt ctgttttcgg cgttgctatc ttgtaaataa tttgaatcca
      361 ccttttaggt ttatataagc aaaagtacaa atattttatg gtaataaaag tctataaaag
      421 aaaagaggat tgctttggca atcctctata aaacaatata atcctgaaaa ttaatctctt
      481 cttctcaaaa tgagtgaaat ataatataat aaagtaacta atgaacttaa agctgctaca
      541 acataagtca ttgctgccca ttttaaggca tctttagcac cgtctaattg gtctgctctc
      601 ataatatttg cttgatctat ccaatgaatt gctcgtgctg atgcatcaaa ttctactggt
      661 aaggtaatga aagaaaataa agttgttaaa gcaaacaaag caacaccaac tactaaaaca
      721 tagggattat tcgtcactgc tagaactcct aatcctgcca aaataatcca ttgcatatat
      781 tttgaactta tttcaagcat tggtaccatt ttagaacgca ttgttagcca gttatatgca
      841 gttgcatgtt gcacggcatg tccacattcg tgtgccgcaa ctgctgctgc tgcaacatta
      901 gattgtgcat aaacccaatc acttaaattg acagttttgt tcaaaggatt gtaatggtct
      961 gttaatgctc ctggaactga agttactgtt acatctgtaa ttccatattg ttgcaacatc
     1021 atttcggcaa cttcttttcc tgttagtccg attggaattt cagaatattt tttgaatttg
     1081 ctttttaatc tgtaactcac ccaccagctt acgcccatga tgacaaacat taaaatatac
     1141 aacattcctg aattcataat ttataaaatt tatctgggac aaaattactt caaatttttg
     1201 atgtaatctc tgttttaaca atatttaatt aacttttttt agatagttaa aaaatgaaaa
     1261 acaatagcta taattttaga tatcgccacg taataatcta tatctttttc gtgcttcgat
     1321 aacaaaggtg ctgtctttgt attctaaaat tattttctca taataatttt gtgctttaag
     1381 gttatcattc aagaagtttt ggtataattc tgcagcataa aaaatagcat cgtctgctaa
     1441 aatatcagtt ggatattgct tttctatttt taataaatat tctaaagcct cagaatattt
     1501 ttgttgtttc ttggcaattt tatattgttc aaaatatata tcgtcaatta acgatgaatt
     1561 gcttgcatgc tgtaataata atgcaaatga atctttggct gcatctaatt tattctgaaa
     1621 ttctaataaa ttggctcttg aaaatgctaa taaaatttca tctgtatcgt ctgtatttag
     1681 gttgtctaaa ataaatacag ataaatctat tgcatcattt gaaattaatt cagttgtatt
     1741 ggcttttata atttttaatt gcgtttgagc ccattcaaaa tcgcctttaa aataagacaa
     1801 acgagcattt ctaaatttag cttcttcgcc aagagctgta cccttttggt ctttttctac
     1861 ttgtgtataa agtaatcgag catcccaagg attattttcc ataatataat aatcgcctaa
     1921 attcaatttt gccgtggcga tagttgtcgc ttgtaatcta ggatttttta ctaattcttc
     1981 taataactcg atagcatctt gggtattgtg taaatacaaa gcttctaaac ttgctaaatc
     2041 aatagttgca ttatacacca tttgattatc tgcatttgaa tttataaagt ctaaataatc
     2101 tacttttaat tttagtaaat cagtttgtgt ataattcgtt tggttcgtta ttttttcttt
     2161 ttgagcatta atcaaagaaa ggcttgctgc ataataccaa ggattattat ttcccttttg
     2221 gacaatatac tgaaatcctt taattgctgc attgtaatct ttttgttgta aagcagcatt
     2281 agcaatcaca aatatattat tgccattttc attgcggagt atatccaaag attttgcttg
     2341 aataattgca ccttcaaaat cattgatttg catatacaac caaaccaata aatcttgata
     2401 aaccaacatt gttttatctt ttccaattcg ttggtaaagt gatttttcta tttcatcttt
     2461 tatcttatca tttttattta tggcttttaa taaaccattt tcaataattt ctaaattatc
     2521 tgcttgtgat ggtgcttcgt ccaaataagc ttgcaccatt ccagaaatat tatcttgata
     2581 taaataaaga ttggctattt gcaggttaaa ttcatacgga ctttttaaaa tttctttgcc
     2641 ttttaaataa gttttcttcg cttcttcaaa taaattagat tgttcaaaat aattggcaat
     2701 acctaaaata tcaaatttgt ttttgggtaa atctttgatg attttggtat aaattttttc
     2761 tgcttctgtt ctattatttt gttgctgata aaatattcct aaatctatta aatatattgg
     2821 attgttatag gattttttat aggctgcttt tattagtttt tcaatatttt ctttgtttcc
     2881 aatcttattt agaacggcat aatattcttt ataatattgt aagccatcag cttgattgaa
     2941 taaatcggca taatattcag cagctttttc gaactcctta ttttctacaa attgacgtgc
     3001 taaataatga ttataatttc cttgttgggc ataaagttgc acagcaaaaa atagcattaa
     3061 aaaaaacaat agtctttttg gattttgcat catcaagatt aaaagtaaga aaaataggga
     3121 atattaaacg ttaaattagc ttttttaatt cgccaaatca ctaataaact taatacgcat
     3181 caattgaatt tcatcgtatt caatatcatc ttcttttaaa attttatagg ctatttttaa
     3241 atcagtcgtt tctgcttctc tgaaataatc aaaaatttcc tctcgtatat cttcatcaat
     3301 tttttcattc aaataataat caatattgac tttagttcct gaattaataa tagtttctag
     3361 ctctattatc aagctttcca tattcatatt gtaggtagaa gcgatatgtt ccaaaggcat
     3421 acgtttatca attgctttaa taatattgat tttagtattt gaatttttat ctgccgtttt
     3481 tattgttgaa aaatcagaat atctttcgat accattttcc tcaatatatt tttttatcgc
     3541 ttccaaaaaa tctttaccat atcgttcagc ttttccttta tttacaccaa aaatatttga
     3601 aagttcttct gtggtaattg ggaaaaatgt tgccatttct attaatgaag cttcttgaaa
     3661 aacaatatct gttggaactt tatgttgttt ggcaactttc tttctgatat cttttaatac
     3721 atctaacaat aatggttcta aaacattggt atttttttga gtgcttgtga taatgtcttc
     3781 atcgtcctca tcttcttcaa atttatggtt caaaacacct tttatagatt cttgtttttt
     3841 taagaatttt ttgccttttt cagtaagttt aagaattcca aattcttcaa tttctttgcg
     3901 tagtaaattt tccaaaatgg ctttactcac cacagaactc caaaacaaca tatctttatc
     3961 tgaacctttg ccaaaaatct tgagtttgtc atgatttttg gttgtgattt ctgctgattt
     4021 tctacccact aaaatattca ctaaattcac agatgttgtt tgttcgttag tttctaacaa
     4081 tgcttgaagt gctaataaaa tttcattttt aatttctatt ggctctttag gtttgatgca
     4141 attatcacat ttcccgcagt ttacggtctc ttctccaaaa taatgcaaca caattttttt
     4201 tctacattct gcactttgag caaaggtttc cacttcagcc aattgttgta ctgcaatttc
     4261 cttttctgtt actgattttt ccttattgct cagaataaat ttttcaaatt tatgtacttc
     4321 ttttggagaa aaataagcaa cacaatctcc ttgcaaacca tctctacctg cacgacctgt
     4381 ttcttgatag taattttcaa tagattttgg gatatcaaaa tggattacaa agcgtacatc
     4441 tggtttatca atacccattc caaatgcaat agttgccact atcacttgca aatcttccat
     4501 caaaaattgg tcttgtcttt cactacgtgt attggcatct aagccagcat gatacgccac
     4561 cgcattgata ccattaacgg ctaaaatttc ggcaatttct tcagtcgtat ttctgcttaa
     4621 acaataaata ataccactac ttccgttttg ttttttgatg aatttgatga tgtctttaac
     4681 agtatcttgc ttattctgtt ttagttttat ttcgtaatac aaattttctc tcaaaaatga
     4741 agatatgaac acttcaggat ctttcatgcc taaggttttc aaaatatctg cttgaacttt
     4801 tggtgtggca gttgccgtca atgccatgac aggaatattt ttgtcaattt cattaagcat
     4861 ttgcctaatt ttacgatatt ctggtcgaaa atcatgtccc cattctgaaa tacaatgagc
     4921 ttcatctatc gctacaaagg aaatagaaat tgttttaagg aaatcgatat tttcttgttt
     4981 aataagagat tcaggagcaa catacaaaag tttagtaata ccagaagtta tatccttttt
     5041 tacagtatta atttgtgttt tattaagttg agaattaagg aaatgtgcga tattatccac
     5101 attgctataa ccacgaatta aatctacttg atttttcatc aaagcgatta aaggagaaac
     5161 gataatcgca gtaccttcag aaactacggc tggtaattga taacataaag atttacctcc
     5221 gccagttggc ttaataacca tagtatcttt tttggacaaa atactttcaa tgattttctt
     5281 ttgattgcct ttaaatttat caaagccaaa ataatttttt agctcttgtt ctaaattgaa
     5341 tgttgtttgc ataaattctt ttaatcctaa agagaaaaca ttgtaataca atgctttcaa
     5401 aataaagata atcaaaaata agaataaaag caagtatttt ttatagggtt tcgtttatag
     5461 aattcttaat aaattgcata acttcgccat ttcaataata ttagtttaaa attacacaca
     5521 atatgaactc aaatacttat gttgctatca tggcgggagg aattggaagc agattttggc
     5581 cagcaagtag aacatccaca cctaagcaat ttttagatat attaggaact ggaaagagct
     5641 taattcaagg aacttatgag cgatttttaa aaatttgtcc taaggaaaat atttttattc
     5701 ttacacattc tgattatata gatttaatta aagaacaatt accttcaatt acaaataatc
     5761 aaattttagc agaaccagca cgaaaaaata cggctccttg tattgcgtat gctgctttta
     5821 aaataaaaaa gattaatcca aatgccaata tcattgtagc accttcagat catatcattt
     5881 tgaatgaaga tgaatttgtt cgtgtatcaa atttagcttt agattttgtt caaaaagaaa
     5941 aagcattggt aactttaggt atccgtccaa cgagaccaga tactggctat ggttatattc
     6001 aatatataga aaaagaaatt caagcagaaa tccataaagt aaaaacattt actgaaaaac
     6061 caaataaaga agtggcagaa acttttatag aaagtggcga ttttttgtgg aatgctggca
     6121 tatttatttg gaatgtgaat tcgattattg atgcttttga gcatttatta attgaagtat
     6181 atgatgcttt tcaagaaaca gaaagtcaat tgtgtactga aaatgaaaat gatgctattc
     6241 agcgtgcttt tatcatatcg ccgagtattt cgatagatta tggtattttg gaaaaggccg
     6301 ataatgtata tgttgtacct gcatcatttg gttggagtga tttgggtact tgggcatctt
     6361 tatattctga aaaggaaaaa gattttatga ataatgctgt ttcaagtgat aatattattg
     6421 tttacgaatc ttataataat attattcagg cagataaaga taaattggtt gtggtgagtg
     6481 gtttagaaaa ttatattatt gtagatactg atgatgtttt gttgatttgc aaaaaagagg
     6541 aagagcaacg cattaaagaa tttacttatg atataaagtt gaagaaaggc gaatcttact
     6601 tataattcct aaattttaga caattcttct tgcctgagat ttatcagtaa ttgatttatt
     6661 ttctgtatga ttgatatgaa caattccagg agtttttcca agttctaaag taccaaattt
     6721 ttgttgccat cctaaggctt ttgcaccatt aatagttgcc caatttaata ctgtttcaaa
     6781 tgatagatat gaacagtatt ttaaaattgt ttttatttct tccaaaatat tcaattgcca
     6841 gttagatgtt aaactgtctg ttccaataca aacttgagct tttgtgtcga taaaatattg
     6901 atagtttggc aaactatttt cgatatacaa attagcattt gggcatgtac accaataagt
     6961 attttgaaag cctaattttt tttgagcaag taaaatatct tcttttgtgg tacaagtatt
     7021 atgaataaat aaatttctgt ggtgtggttt taaaaattct aaagcataat gaatagaacg
     7081 caaatgtttt ggaattggtt ctaaggtata atttaggttt ttataaaaat ccacaaactt
     7141 gcctgattta ttaaaaaata aagcatcttc cgacaaagtt tcttgattgt gtatagaaat
     7201 ggttttgtct gtattattat caaaattttg aattaaataa tcgaataatt ttggagaaac
     7261 agaataaggt gcgtgtggca cttttgattt tttattggtt tcactttctg ctaattgttg
     7321 ataaactgca tcatattgct caatagttgg tagggtattt atattttgaa acatatcaaa
     7381 atattctacg aaagtatagt aatgtaaatt acctttttgt ttttgttcaa aagtatcaac
     7441 tgcatttgaa atatcaccaa ctgcaacaat tccattttga agcatttcat tttcagctgc
     7501 tatgattttt gcttgaataa tttcctgact atggtttcta tttttaataa cttgtcctat
     7561 aaaatctatc aaacctgtac ctgtttgtgc aacatttttt aaatgggaca attctaaatg
     7621 acaatgagca ttaacaaaac ctggaactaa aaaaccttca taaatttcta attctgaatc
     7681 atcataattt ttatcctcat caatttttac aacttcattg ttggtattca atataataac
     7741 accgttttca acaggagctg tggatatcgg aaatacaaaa tctgctttta gttttcgata
     7801 catagcttaa ccgttttctg ccactacttc ttgcacttct ggaataaatt ttttcattat
     7861 attttctatt ccttgtttta aagttacagt agatgatgga catccattac aagaaccttg
     7921 taattgaaca gttaaaattc catttataaa acttacaaat gtaatatttc caccgtccat
     7981 ttctacagca ggttttacgt atttatctaa agcagtttta attttaattt ctatatctga
     8041 gtcattttca aaaatagtat tggttgcagt tttttcaggt tttataaaat cttcggctat
     8101 aattggtaaa ccattttcta tataattttt aagaaatgat ttgattttcg gcattaattc
     8161 ataccaatca ataaaatcat cttttgcaac agaaacaaaa ttattactga tataaacaga
     8221 agtaatttgt tctatactaa acaacgcttt agctaaaggt gctttatcag tttcagaaac
     8281 agattcaaat tcagcaaaat cattaggata taatggtctg ttgataacaa atttaagtgt
     8341 atttggattg ggtgttgatt ctgtataaat catcaccgga atattagttg ttgtttccat
     8401 ttttaattta tttaattcct gcttgtgctg atatttccat cattctatca atagattttt
     8461 tagctgctaa tcgcaaattt tcatctataa taatttctgg catttcatat tttaagcaca
     8521 aataaatttt ttctaatgta ttcattttca taaatggaca atcattacaa gcacaagcat
     8581 tatttggtgg tgcaggaatg aaagttttat taggtgaagc ttgcatcatt ttgtggatga
     8641 taccaggttc tgttgctaca ataaattctg ttgcttcatc tgttgatgta tattttaata
     8701 aagctgaagt agaaccaata taatcagcaa catttaatac agcttcctca cattctgggt
     8761 gagcaatcat tttagcattt ggatgttttt cttgaagttt tgtaattttt tctaaagaga
     8821 aaatttcgtg tacaatacaa gagccattcc ataaaaccat atctcttccc aattttttag
     8881 aaagataagc acctaaattt ctgtcgggtg caaaaattat tttttggtct tttggtaaag
     8941 tgttgataat tgtttcagca cttgaagaag taacaatata atctgaaagt gcttttattt
     9001 ctgcagaaca attgatataa gacaaaacca catggtcggg atattgcttt ttaaactcag
     9061 caaatggtcc tggtaaacaa ctatctgcca aactacaacc agcatgtaaa tcaggtaaaa
     9121 ctactttttt ggttgggttt aaaatttttg ctgtttctgc cataaaatga acaccagcaa
     9181 aaacaattaa atcggcatct gttttttcgg catattgagc taattgtaaa ctatcaccaa
     9241 tataatcagc aacatcttga atatcagcat tttggtaata atgagccaat aaaactgcat
     9301 ttttttcttt tttcaatttt tgaatttctt caaataaatc taaggttgga tcaatatcca
     9361 tatcgataaa accacgttgt ggaagttctg caattttttc ttttaaatcc atacttgcta
     9421 tattttccat tttttgaaaa ttttgttcac acaagatacc ccattttttt tgaattttag
     9481 aaaatctaag ttttagaaac tatgactaat aaaatataaa ttaattaaaa aaaattatta
     9541 ttacttattt attacctctg tgcattcgtt gatacttttt tttcaatatg ttttgttttt
     9601 tgttttttat aattaataaa tgagtttatt cacattgatt aaacatttta tatctattaa
     9661 tgttctactt tattttcaaa gacttatatt ttttatttgt ccttaatacc aattttacac
     9721 acaagtacta tttatttttt ctaacatact ttgtggattc ttttttttct atttaatgtt
     9781 tatttgtttg aatttttagt gcttaaattt ctaataagtc atcttgctcg tggagatttt
     9841 tttacattaa aattactttg cacttaaaat aaattaatac acaaattaat tgagttttaa
     9901 acataaaata ttgttagtaa gttgagcttt tttcaattgt tttgtacgaa tactaacaat
     9961 attgtgtata tatttatagt tacttttatt actaataata aaaaatatta tctaatttat
    10021 tttgaaagta attttttata gatagatttg attatcaact atataatttt ctactttttc
    10081 aggtataaaa taaagaatgc ttttattttc ttttattctg tttcttattt ctgttgatga
    10141 aatatcaagt aagggtaaat tttgaatttt aaaataatta tcttgattat tgacgcttga
    10201 cgcttgattt ttatttctat tatacactaa tatctcaacg ttcttttgta tccattcagt
    10261 ttctttccat tcgtggaatt tttctatatt atcagcaccg ataattaatt taaaatcata
    10321 cttaggataa ttttttttca attcttttaa ggtaagatgt gtataagatg gtttaggtaa
    10381 attaaattct atatctaaaa ctttaaaatt tgatataggc tcaatggcta attttaccat
    10441 ttctaatcga tgattttcag gagctaaatc aacatttttt ttgaaaggat tttgtggtga
    10501 tacgataaac caaatttcat ctaaatttgc agcttcacga atatgtatag caactaataa
    10561 atgaccaata tgtattggat taaatgatcc aaaatataaa cctattttca ttgttttaga
    10621 ttttcttgtt ttcctaaaat ttgaattaat ataaatatac ttaaaccaac taattgttct
    10681 gttaaagcat cccaaaagca aaccattaaa aatagactat ttaatataaa taatagctta
    10741 ttatttatcc aatattcttt taaaataatg attattgaaa atgtaaataa aataaatcca
    10801 acgacaccag tactcactaa aatttgtaaa taagaattat gtacatacat cattttagaa
    10861 gcatcaaaat atttatagat agcattgtat ttattttcag tttctttttt tatattagtc
    10921 aatccaacac ctaaaagttt attttcttta aaaatattcc aagcaatttg aaaggaaact
    10981 attcttcttg catctgaata atctaataca ttttcttggt ggagttgttg tatatcatat
    11041 tttaaataat tgattttatt ctgtaaatta gtactaaata aataacctat tactggaaaa
    11101 agtaaaataa ttatatattg atatttcttg taatatattt tttgaatgat ataaattatt
    11161 atagaaatat atagtattaa taatccagat tttactgcca aaatatgtaa gtatattata
    11221 aagtatagta taattatatt tttaaactta ttgttgataa tatttgtata agttagaata
    11281 tttatactta atacgattag taaactgaat tttacatggt gcataaaggt aggtaatact
    11341 tgacctttag aatataaatt gttccaatag tttttatcgg aaaaatattg gataatagta
    11401 taaaatgaaa ttccaattaa aaagaaaagt attaaattga aaagaatttt tttttgctga
    11461 ttacttataa ttttaattga ataaatgaac gcaaacccta ataaactaat ttttgaaatt
    11521 atagtttgga gactatctaa tatattattt gttttccaaa aagcaccaaa taaataaacc
    11581 atgaataata aaagaaatcc taatggtaaa ttttctttta ggtaaatttc tttaaattta
    11641 tttgttatta cccaagcaaa aaatagtagc gtagaaatac ttaaaacagc attactaaaa
    11701 aataagccaa ctacaaaagt agaaagtagg gcaatattaa tgttttgata tattgtttca
    11761 ttaattttca acacattaaa aatagaaatt ttttacaatc ttcctaaacc tactctcact
    11821 gctacattga aatttagata actaaacgaa aaatcatttt tcttgatttt tgtaattcta
    11881 tcgtaataga aagtaggagc gtagtcaaat gaaagattaa atggtacaat taagttttgt
    11941 gtccaaaaat ctaaacccac cacaggagca agaccaccag aaataatagc ttgttttggt
    12001 gcattttttc caaaacctat atttccacca ccaccaacat aaacaaacca tttgcctctt
    12061 ctgtctatct caaaatgttt ttgataagct gttccaaaat ttaaaactga agtattgtat
    12121 aatgtaccat gaacaccttg tttcacagta aattctaatg catttccatt tctatctaaa
    12181 aagtattttc ctgataaacc ccaaccataa ccaaagcgta aaccaatagc tcctttactt
    12241 tcttgggtaa aagaattggt taggctaaaa attaaaaata taaatagaaa attttttagc
    12301 ataaattatt taatacaaga taatattttt tctcttaaaa tagagtttta taatttttta
    12361 gcttaatcat ctattcttgt actactcaac caaaagttgt tatttccttc acctaatact
    12421 aagaaaatta cttgttgttg gattaactct aacataccta aaaaaataaa aacagcatgc
    12481 attttatttt cacaagcagt taaaatttct tcaaaggaac tttttttctt ttctttaatt
    12541 aaagatgcta atttatttct ttcttcttca atcgtatatt cgtactgaac aattttatgt
    12601 gtaatttttt cagttctagt tttgtattta tccaatgcac gttgataagc acgcatcaat
    12661 tttatcaaag aaaccgtttc cagttcaaca tcatcagcta agattttaat taaatcttca
    12721 acttcagctt tttgattacc acgagtatgt cttaaactac gttgagcttc taaatcttta
    12781 atttcttcaa caacctcttt aattttttta tattccacta attgttgaat aagttcttgt
    12841 cttgggtcga tttcatttcc ttgctcatct aattcttttc taggcagaag cattttagct
    12901 tttattctca tcaaagatgc agcaactaac ataaattcac tggcaacttc aatatttaat
    12961 ctttctaaat gatggatata attcaaaaaa tcttttgtga ttttgaaaat tggaatatct
    13021 tcaatatcta attcatctct ttcaataaag aacaataaca aatcgaatgg tccttcaaat
    13081 tgtggtaaat ggattaaaaa aggagctgac atatatttta tttacttaga atgtttattt
    13141 ttgaatactc taagatacat acaagttgtt ttttttatgc aaatagttat ctacacaact
    13201 gaagttacta acagactaaa atatgtttta gattttatat ttgttcaata ttttggaata
    13261 gattattttt tagaaacaga tattgttgtt tttaaaaagc aaactttaat tgcaaatttt
    13321 atttgtataa attattccaa agaacaaatt aataattgta taaatatttc ttgtgaaaaa
    13381 ttactgtttc aaacaaatat aatacctcaa aaaatattta ttgcactgat tgatgaaatg
    13441 cctgttatat ttccttcgga atttctggac tctagtttcc aatttgatat ttttgcggct
    13501 atattttatc ttataactcg ttacgaagaa tatctacctc atgaacaaga tgaacatgga
    13561 cgttattgtt cgcacaattc aattttagct aagcctgaat ttagttttaa acccatagtt
    13621 gaaatttggt tagcttattt taaatccatt ttattaaata ttcaaccagg tttagaattt
    13681 aaaagacata agtttactaa gatttttact tttgatattg ataatgcttt tcaatataag
    13741 catagaaatt ggtttaagca tccacctaat atatttataa cagaagtaag aaatgtgttg
    13801 tttggaaagc aaaatgacaa gtataataca tttgatgaat tatttcaatt ttgtaatcaa
    13861 ctttcccata aaccttattt cttttttcta ttgaatgaca gttctaggag aaatagcaat
    13921 gtttctccaa aagccaatgc attacaccat attatcaaaa attgtacaga attttatatt
    13981 ggtatccatt catcttatca tttttcgcaa acttttttta ttcgcgaaat acaattgtta
    14041 tcaaaaattg taaactatcc tattgttttt tcaagacaac attttctaaa aattagtttt
    14101 ccggattatt ttagaacact tgttgagtat ggcattacta aagatttttc tttaagctat
    14161 ccaaatattt caggatgtag agcaggtact acacaagcct ttttcttttt tgatataata
    14221 aatgataaac aaacatcgtt actaatacaa ccatttattt ttatggatgc cacctatcaa
    14281 tattatattc acgatgataa ttatactatt caacataaag tagaaaagtt attagatgaa
    14341 atacaaagaa taaacggtaa ttttgtatcc ttatttcata atgatttatt acatgatagt
    14401 ttacccttta aaaacattat gaaaaatatt caagaatatt aatagcaaag tatgtttaat
    14461 tatatatata gaggtgtttg gattttattc atatttttat tttcttttgt acaattatat
    14521 agtcaagatt ataagtttgc acaatattat aattccccat tgaacctcaa tcctgctttg
    14581 acaggtaaaa taaatagttt ataccgttta acagcaaatt atagaatgca atattttact
    14641 gttcaagcac catcacctta tacaacaatt tctggctctg ctgactttgg tttatttcga
    14701 gataaattga ataatgatat tttcggactt ggaattgttt ttggaaatga tagacaaaca
    14761 atattgcgaa cgaataaact agatttatct gcagcttatc ataaaagtat tggctataaa
    14821 aaacaacatt attttagtgc agggttgcaa attggattta tacaacgaac aattgacttg
    14881 aataatctat attttggtgc tcaatttgat gaagatagat ttgatttaac aaagccaaat
    14941 ttagaaaact tcaattctaa taagtttatt aagcccgatt ttaatcttgg tttattttgg
    15001 acatcttcat tgaataaaga caaaattggc atttttgctg gagtttctgc atttaatatg
    15061 tttttaccga atcaaagttt tttatcttca aactatgaac gatctatgcg ttttgttgtc
    15121 tattcaggtg cgtcattcca aatcaagaaa tttatgatga ttattccaaa tacattattt
    15181 gcattacaag caaaaagtat tgactggagc cttggatcta attttgcttt taatatttcc
    15241 gaaaagcgat atgcttttaa atcttctgta ttgtttggtg tgttttataa tggaaatggt
    15301 ttaatatcta caaatgtagg aattcaacat caaggattta tggcaatgct aggttatgat
    15361 gctgatttag taaaagatat taacaatgct gttaagtctg taggtgcgat ggaaatttct
    15421 ttgatttata ctggaaatcc tattaaatcg aagatatatc aacctttatt ttgtcctaag
    15481 ttttaatttt gattgttcca cgtgaaacac taacatttta taaacatcaa acttattaag
    15541 atgtttatag ttttgtgtat tgtttgcttt gtttgactat actctttatt tttcttagga
    15601 tacaaaattt ataatttttt tcttattaag ttatttattt caaagcaagt tgtatattta
    15661 ttctcgattt gctcattttg tttcacgtga aacaattttt tcttctcctt ttaatacttc
    15721 ttatgtttta ttgttcttta ttttattgtc tttgctttat attttattat agaaactttt
    15781 ttcaactttg aatattatca attttaattt gtgatattat actttggttt gttccacgtg
    15841 aaacaattca catttattcc acatgtttat tatctacatg tttataattt tgttgatgag
    15901 caaaatactt agtatttgat ttgttagttt gattgttttc tcttttggtt caatttaagc
    15961 tataaaagtt aagtattcta tatttttttt atctgtttat atattttctt agatattcac
    16021 ttaactttgt attatgtttc aaacttatga tgttattgta gttggtgctg gtcatgctgg
    16081 atgtgaagcc gcagttgctg ccgcaaaaat gggtagtaaa gttttgttgg ttaccatgaa
    16141 tatgcaaact attgcacaaa tgtcttgtaa tcctgcgatg ggtggcgtag caaaaggtca
    16201 aatccttaga gaaattgatg ctttaggtgg atattcaggt atcgtttctg ataaaactat
    16261 gatacagttt agaatgctta atctatccaa aggaccagcc atgtggagtc ctcgtactca
    16321 aaatgataga atgttatttc atacaacttg gcgtgaaatg ttggaacaaa cacctaatat
    16381 agatttttgg caagatatgg caaatggttt aattgtagaa aatggacgtg tttgtggtgt
    16441 tgaaactagt ttaggtatta aaataaaggc taaagcagtt gtattaacca atggtacttt
    16501 cttgaatggt attattcata tcggtgaaaa acaaattggt ggtggaaggg caggtgagaa
    16561 agccgctaag ggtattaccg aaaatttagt gcgtttaggt tttgaaagtg gtagaatgaa
    16621 aactggcaca ccaccaagaa ttgatggtcg tagcttagat tattctaaaa tggatataca
    16681 aaatggcgat gaggctaaat atggtttttc ttataaaaat atatcacgac ctaaagaaca
    16741 acgttgctgt tggattactt ataccaatga aaatgtgcat gaacttttaa aagaaggttt
    16801 tgacaaatct cctatgtttc aaggtcgtat tcaaggaatt ggtccaagat attgcccttc
    16861 tattgaagat aaaattaatc gttttgcaga taaagataga catcaaattt ttgtggagcc
    16921 tgaaggttgg aataccgtag aaatttatgt gaatgggttt tctacctctt taccagaaga
    16981 tgtacaatat aaagcaatac gtcaaatacc aggttttgaa aattgcaaaa tgtttcgtcc
    17041 tggttatgct atcgaatatg actatttccc acctacacaa ttgcatgttt cgttagaaac
    17101 taaagcaatt aaaaatttat tctttgcggg tcaaattaat ggaacaacag gatatgaaga
    17161 agctgctgca caagggacaa tggctggtat aaatgctcat ttagcgatta atgataaaga
    17221 acctttaatt ttaaaacgtt cagaagctta tatcggtgta ttaattgatg atttagtgaa
    17281 taaaggaact gaagagccat atagaatgtt tacatcaaga gctgaattta gaatcttatt
    17341 aagacaagat aatgcggata ttcgtttgac agaattgtcc tataaattag gttttgcaga
    17401 agaagaaaga atgaaaagag ttgaagaaaa gattaatgcc acaaatgaga ttagaaattt
    17461 tgctaaaaat tatagtgttg aaggtaaaga tattgacact tatttagagg atgtaggaac
    17521 aacaaaattg tatcaaaaaa caaaattgga taaaattatt actcgccctc aaatcaattt
    17581 attaccttta gtagaatatg ttccatctat taaacaacat ttggaaaagt atgaagagga
    17641 gtttcttgaa caagccgaaa taaatattaa gtacgaagga tatattgaaa aagaatttga
    17701 gcaagtaaaa aaacttgaaa aattggaaga ctataaacta aaaccagatt ttgattacaa
    17761 tcaaattaaa tcactttcta tagaaggcag acaaaaattg aataaaatta aaccattaac
    17821 tattggacaa gccagtagaa tttcaggcgt ttctcctgct gatatttctg ttttaatggt
    17881 ttatttagga cgatagttga agtactataa tgatgaataa tgaaatgata ataaataaag
    17941 gtgcttttat aaatggaatt tctagattcc tacatactca tttacgctca tttctttatt
    18001 tgtttattct ttcttttcta atgaatgcat gtgccgttat ttctgctcca actggcggag
    18061 aaaaagataa aattgcacct aaagctatta aaattaaccc acaaaatgag acagtaaatt
    18121 ttaatgctaa agaaattaat atagattttg atgaattcat tcaattacag aatccccaaa
    18181 atattctaat tactccagac attaacccca aaccagtttt tacttcaaac agaaaatctc
    18241 ttaatataaa attcaaaacc acattagata gtaatacaac ctattcaatt ttttttggat
    18301 cagaactaaa agattatacc gaaggtaatc caaccgataa ttttacttat gttttttcga
    18361 caggagaata tattgattct ttaaaaatta aaggtagtat tcagaattat gaagataaat
    18421 tgccacaaaa tacttatgct attttatata aagatttagg agattctgtt tttactacaa
    18481 aaagaccaaa ctatatatca agaattcaaa ccgatggtac ttttgaaata aatcatctga
    18541 aagaaggtca atatcaaatt tttgcattag cagataataa tagcaactat tattatgatt
    18601 taccaactga agcaattggt tttttaaaag aaaatataca agttgattca aattctcaaa
    18661 atattatatt accacttttt attccagaag tagcacaaat cagaattata gaaaaagata
    18721 aagcaattca aaatggtatt tttaatatta cttggaataa aaccttatct ccaaaaacag
    18781 atcagtttga aattgtaagt ttgccagaaa ccaaagcttt agcatttttt gaagacaaaa
    18841 caatgcgaat ttattttcct aatctacaaa cagatactgg gcatattcag ttattcgtga
    18901 aacataacgg gaaaattgtt gattcaatag aaactaaatt gaccagtaaa aaaacaagca
    18961 atatcttttt tgattccatt caatacaaaa gtataaagca attgcaaagt aatcctttaa
    19021 aattagttgc atctaaaatt tcaattgctg atattgatac aaataaaata tatattattg
    19081 atacctctga aaataaaata cctttttcaa ttactcaaga ggaagatttt aaaacgtata
    19141 aacttactgg aaatttctta cctcaaaatt atacaatcta catcttagac tcttgttttc
    19201 ttgatttagc tggaaatttc aatacagccc agcaaatttc actctccgta cttgataata
    19261 aaaaaggagg taacttattg ataaacatag aattagatac tatataccca aatttaattt
    19321 tcattttaaa agataatcaa gggaaagtcc tgaaaaatga atttttaagc aattctcaga
    19381 cggttaaaat taacattggc ttaatacaag caggtgagta taaagtcgag ctaattcgtg
    19441 atgataacca aaatgaaatt tggaatagtg gaagttttat aaataaaacc ttacctgaaa
    19501 aaatttatta tcacccaaca ccaattattg taaaagaaaa ttgggatgcc gaagagacca
    19561 tacaaccaaa atttgatgaa gtaaaaaaat caatacttaa tttacaaaat aaaaaacaag
    19621 agttgaatca gcaaattaat aacaattctt caataccatt tcaaaatcga aagctaaaat
    19681 aataacgttt cttcttttgt tttgtcttaa aatatgttaa tctttatata ttacataaga
    19741 tttttaattt aaacattgat tttctgtaac tatttttcgt agttatcgta tatctgtgct
    19801 gtaacatttt ttatccaagt agcataataa tgaaagaaat aacacaatta atatttgatt
    19861 ctaatttaga caatatagca aaagtagaac aagcgataga agatataaaa caaaattata
    19921 ccattacaga tacgatttac ggcaatgtta tgattgcttc cattgaagca gttactaacg
    19981 caatagaaca cggtaatagt tttgataata ctaaaaatgt attttttaaa gcctacattt
    20041 gtaatcaatc cttaaaaatt tctgttgaag atgaaggttg tggttttgat gtggatactt
    20101 tgccagatcc aacgacaatc gaaaataaag gaaaccctga tggacgtggt gtatttctga
    20161 tgcgacactt agccgacgac atcaaatttg aaaacaatgg cacacgtgtt gaactatttt
    20221 ttcggttgaa ttagctaata attttttcgg aattattcta ttttaaaatt gtgaaatatt
    20281 attttaaata cggaaaaaca aaagccgttt taaatttttt ttaagttgaa attagacaat
    20341 atgaatcgag ttatgtgaat atttctaact aaagatttta taatctcctc ctaacaaaag
    20401 ttaattaatc taccgaatag atacagatgt tccaattatt ttggtaactt tatattaaca
    20461 aaataaatga aatatgtcaa agataaaaga ggctaacgtt ttagttactg gaggtggtaa
    20521 cggaattggg aaacaaatgg gcttaaaatg tttgcaagat ggttgtgcaa atttagtgat
    20581 atgggatatt aacaaagaca atttagataa aacaaaaaaa gatttttcca atcgtggatt
    20641 taaaaatgta catacttttt tagtcgatgt ttctgatatc gatgatgttg aaaaagcagg
    20701 aacagaagtt ttattagaaa ttggaaatat tgatatctta attaataatg ctggtattgt
    20761 agctggaact aaaagttttt gggattatac cgcaaaagat attgacagaa ctgttgatat
    20821 caatataaaa ggagtgatgt atgttactcg tgtttttatc aaagaaatgt tgagacagaa
    20881 aaaagggcat gttgtcaata tcgtttcggc atctgcttat ttgggattgc caaaaggctc
    20941 tgtttatgct tcaagtaaat ggggagcaat gggttggagc gaaagtttac gtttagaatt
    21001 ggagagagaa gaaggtgatt tacacgtaac agcagtttgt ccaagttata ttgatactgg
    21061 aatgtttaaa ggcgtaaaag ctcctctttt atttccatta ttagaaccag aaaatactgc
    21121 agaaagaata attaaagctg taaaatctaa tcaagtgcat ttattattgc cagatacttt
    21181 aaatttagta cctgtattaa aaggcatttt ctctccaaaa atatttgacg tagtcgccga
    21241 ttggttggga gtatatactt caatgaattc atttgaagga agagcagaaa aagaaagaat
    21301 tcctgaaagc aaaaaacaaa agtcaaaata atacgtgggt attaaaatat aaatgctatg
    21361 gcaacaaaac aagcaagaaa aacaggacaa atccaacaaa tcatcagtga gcaacaagcc
    21421 ttttttgaaa ctgataaaac aaaggatgtt aattttagaa ttgcagcttt gaaaaaatta
    21481 aaagcttcga ttcagaaaaa tgaaggtaag atttatgaag ctttgcataa tgattttagc
    21541 aaatcaggtt ttgaaactta tacttcagaa ataggtacac tttacgaaga aatcggcttt
    21601 ctgttgaagc atattaaaaa ttgggcaatg ccacaagccg taaaagatac agttgccaat
    21661 tttccatcta aaaattatat ctatccagaa ccttatggtg tttgtttggt tattggagcg
    21721 tggaactatc ctttgcaatt aactttaagt cctgtaattg gtgctattgc ggcaggaaat
    21781 acggttattg taaaaccacc acgagcgtct atgcatactt atcaaattac acaacaaatt
    21841 ttatctgttt ttgaagagga ttatatttgt gttttgccag aagatagcaa taacgatgag
    21901 atgttacaat gccgatatga ttatattttc tttactggtg gtgttgaagt gggtagaaaa
    21961 gtagctcgtg ctgctgccga acatcttacg ccaacgactt tagaattagg tggtaaaagt
    22021 ccttgtattg ttgatgcaag tgccgatgtt gatatttctg caaaaagaat tgtttggggt
    22081 aaatttatga atggtggaca aacttgcatt gctccagatt atttattatt gaactcaaaa
    22141 gtgaaagata gtttctatca atcttttaaa aaatatgtca aagaatttta tggtgagaat
    22201 ccatttttta gtgctgattt cccaagaatt attaatgata gacatttttc aagattaaat
    22261 aaaatgttgg tggacggcaa aattattgtc ggcggagaaa cggataagga atcaaaatat
    22321 attgctccaa ctttgataga aattaaagac ctcaatcatc cattaatgga agatgaaata
    22381 tttggcccaa ttttacctgt ccttgaagtg gattctgttg atgaaagtat tgccattatc
    22441 aaacagtttg aaaaaccatt ggcattatat cttttttcta aaaattatga ggaacaacaa
    22501 aaagttttaa agaaaatttc ttttggaggt ggttgtatta atgatacaat tgctcatttt
    22561 gtcaataaag aattaccatt tggtggcgtt ggcaatagtg gaattggtgc ttatcatgga
    22621 aaatatagtt tcgatacgtt ctcacaccac aaaggaattg tacataaagt aacttggcca
    22681 gatgtgccac tgaggtatcc gccgtataaa ggcaaaacac ctattattaa acaaatcctt
    22741 aaataaatat agcctcccaa atctgggagg tttttttata ccattttctt aacattaaat
    22801 tattggaaac tttagatact ttaatagttt ctttagtttt ttgtgtataa ttttgtcttc
    22861 ggttatggaa ggattaaatg aaaacaaaga aataaattta gaattggagg atcaagtatt
    22921 ctgtaaagct aaagatttat ttatgtctat tggtatccga agtacaacca tggacgatat
    22981 tgctaaagac ttaggcattt ctaaaaaaac attgtataaa gttgttgaaa ataaaggcga
    23041 cttagttact caatgtgtta ttcatgatgc aaatgcaaga agattagaag tagatgcaat
    23101 tgtaaaagaa tattctaatc caattgaaca gatgttgcag ttaggcattc atattttaaa
    23161 ctctatcaag caacataaaa tttctataat tcacgacttg atgaaatttt atcctgaaag
    23221 ttggcgaatt ataaccgaac ataaagaaac ttatgtcaaa gagattatca aacaaaattt
    23281 aaagaaaggt atccaatctg gagcttatag aaaagatata aaaattgata tttatgctaa
    23341 tttctttata gcaggtacag atatttgcat cgatacacgt atttttccag aatcagaatt
    23401 tgaatttcct gatattttta gagaatacct tttagctcat ttacgtggca ttgtaaatga
    23461 aagctcattt acagaattaa atcaattaat agaaaaagta aaattttaat atgaagcaaa
    23521 tattcgttat agcatttgcc ttttggagta catttattac agctcaggaa actatgagct
    23581 tgaaagattg tgtgaattac gcactaaaaa atcataccag catttcgatg gcaaaaaatg
    23641 atttagaatc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aatgaagaat
    23761 tgaataaatt aaaattacaa aaaacagaag aagatgtaat ttacaatact gttgcagctt
    23821 attatcaatt attaatcatt aatgaaaatg aaaaattatt gaatgaaagt gaagtgagaa
    23881 tcaataaaac tttacctatt gttcaattgc aatatgacaa aggcgtagca agaaaaatcg
    23941 atgtggacaa aatcaaagta aacctcaata atattttagc caacaaagaa attcttagaa
    24001 taaataaatc tatggcgatg aataatttga aatatacaat aggtatgcca ttagaaactg
    24061 aaattgtaat tgatccaaaa tttgaaaatg aagtatctac tgatatttta aataattccg
    24121 ataatgtaaa agataaagtg gaattgaaat tattaaatac aagtttgatt ttaaatgaaa
    24181 tcaattatag aagacaaaaa gcagcttact atccaacaat tgctttcgca gcaaaatatg
    24241 ctggcaatgc attgggtgat aaattagcag actcttttaa aaaatggacg cagttggcag
    24301 tagttggtgt aaaaattacg gtaccaattt ttgatggctt aagaaattca agtgctttga
    24361 agcaaatgtc tttgaataat gaaaacatta aatacaatat aaaacaaact gaagatggtt
    24421 taaaattgca attacaaaat gcaaaaacag gcattagtaa tgctgttcag aattatacta
    24481 tcaataaaga aaacttagaa ttagccaaaa gtatttttga tgttacaact ttacaatatc
    24541 aaaaaggcgt ggtttcttat actgaagtac tttcatcaga attttcatat aaagaagcgg
    24601 aggttaatta cttacaatct ttagtaaaat acttaaacta taaattagaa ttagataaag
    24661 caaacaataa tattcaatca tacaaataaa aaataaatag atagctatat gaaaactaaa
    24721 atctttttaa ttatcgcagg tttgctcatc gttggtggtg ctgcttataa attagctaaa
    24781 aataaaaaaa tcatagatga caatgcaaag cctatcgaca gaagtggcgt tccaattgca
    24841 gttacagcat ttgaagctgc gtattttcca gtaagttcag attttgcttt gccaggtgtt
    24901 ttagatcaaa ataatacagg aaatgtcaat gcaatcaacc caggtaaaat tgtttcttta
    24961 aatatcgaag ttggacagca agttacaaaa ggacagataa ttggaaaagt agattctaaa
    25021 ttgaaagaaa ttgctttgca acaagcagaa ttgaccatca aaaaattgga aacagatgct
    25081 gtcagaacta aagatttaat tgaagggaat gccgcaccag ccaccagcat tttagattta
    25141 gattataata ttgaaaatac aaaaatccaa aaggacaata tcaaagcaca aattgcaaat
    25201 gataatattt atgcaccaat aagtggtatg attattcaaa agaattctaa tgctggagag
    25261 tttattaatc ctggaactcc aattgcaacg attatggata tttcaatttt gaaagcagtt
    25321 gtttatgtga gtgagaataa tgtgtatgaa ttaaaaatag gacaacaagc acaagtattg
    25381 tcttcgattt atcctgataa aattgttaca ggaacgataa aatatattgc accaagaggc
    25441 gatgaaaatc acaactataa agtggaagta catataccaa atagtggcta caaagcgggt
    25501 acttatgtgt ctgtaaaatt tgcattcaaa aaacctgctg aagctttatt gattccaaaa
    25561 attgctttag tagaaggtgt aaaaaatcct tatgtatatg tagttagtgg caataatgtt
    25621 atcgtgaaaa aaattgaagt tggcgaagaa gttggcgaaa atattgttgt gagaaatggt
    25681 ttagttgctg gagataaagt agtaacatct ggtcaaatta acctatctga aaaaagtaaa
    25741 atccaaattg taaaatctaa tcaataaaat attatgtcaa ttaccgaagt tgcgataaaa
    25801 cgcccattgc tgatttcagt actcttcttg gtattgatat tggctggttt tttaggctat
    25861 aaagaactaa attataactt acttccaaag tttgaagcac cagtactttc tatacaaaca
    25921 gtgtatcgtg gagcatctgc ggaagaagta gaaaccaatg ttactaagaa aattgaagat
    25981 gcggtttctt ctattgaagg tctggatatc atttcttcaa cttcaatgga aaatgcttct
    26041 attgtgatat tgcaactaaa acaaaatacc gttgtaaatg atgcacagca agaagctcaa
    26101 cgcaaattaa ataatgtcaa aaatgaatta ccagatggtg tagatgaccc tgttatttca
    26161 aaattcagct caagtgattt accagtttta aaactcacat tatttgctga taatttagga
    26221 gaatctcaat tatatgattt tgcagacgta accataaaac ctattatttt gaatttagat
    26281 ggcgtaggtc aagcaaaatt aattggcggt agcgaacgtg aaatctctgt gatgttggat
    26341 aatgaaaaat taagaggtta taatttgtct tcagcacaag tgagtcaagc gatatctatg
    26401 ggcaatatgt cgtttcctgt aggtagtatt gaaaacaatt caaaccgttt taatttacga
    26461 ctagatgcca aacttgtttc tgtagaacaa ttgcgtaatt taattgtaag agaaaataaa
    26521 aatggcagta aaattttatt gaaagacatt gcaacggtaa ctgacggaac aatagagcca
    26581 gttactatca atcgtattaa tggaagagtt ggtttgggta tggaaatcaa taaacaaaca
    26641 gatgcgaatg cagtggaagt tgcaaaaatc gttaaagaaa aattagatac tttagttaat
    26701 caatataaat caacaggatt gaattatgaa atcgctgttg accaatctaa atatacttta
    26761 gaagcagcaa atgcagttca acacgattta atcttagcga tttttattgt ggcattagtg
    26821 atgttatttt tcttacacag tttaaaaagt tcattctttg tattggtggc tttgccatct
    26881 gcgatgattc caacttttat gatgatgtat ttgatgggct tttccctcaa tttaatgacc
    26941 ttattggcat tatctttagt tgttggtgta ttagttgatg atagtattgt gattttggaa
    27001 aatatttatc gccacatgga aatgggcaaa aacagagtaa aggcatcttt ggaaggtcgt
    27061 tcagagattg cttttacggc tttggcaatt accttggtgg acgtggtggt atttgtgcca
    27121 ttgtctttgg ctggtggatt gattggaaat attttgcgtg aatttgcact cgtcgtcgtg
    27181 ttttcaacat tgatgagttt attggtttct tttactttaa caccattgtt agcatcaaga
    27241 tttggaagcg ttgcacattt agatgaaaat acattatggg gaaaactcaa tttaaaattt
    27301 gaaagtatta ttgatgcatt aattaatcaa tacgcaaaag cattggattg gggattgagc
    27361 cataaaagat atgttgtttt aggcgtaatc tgcttatttg ctggtgcttt tagtttagca
    27421 ggatttggat ttattggcag ttcgtttgca ggaagtggcg atagaggaga gtttaatctt
    27481 attgtagaat tagatcctca atcaactttg atacaaacaa ataaagttgt gaaaaaaatt
    27541 gaagatatta ttttagaata tccagaagtg gataaagtat ttactaaagt gggtgccgtt
    27601 ggaacacaaa tgggcgaaag tattgccaca ccaaaccaag ccgatattac tactactttg
    27661 attgataaaa ctaaaagaaa aatcacgaca gagcaattga gtatccaatt aagaaataga
    27721 tttgatagta ttccaggaat aaaattctct attaaaccaa ctacggttac tggaaataat
    27781 acgcctcaaa ttcaaattgt tgtgatgaat accaatcatg actcacttca aaaagcaact
    27841 caaatgattt ttgattttgt gaaacaaaca cctggaacag attatgttga attgagcact
    27901 aagaaaagta aaacagaagt tgaaattgta ttggatagag ataaaattgc caaaatgggc
    27961 ttgagtattc ctatggtagg tgcagcagta caattagcat ttagaggcaa tgaccaatct
    28021 aagtttaaag ataaaggcga ggaatatact atcaatattt tatacgataa agaagataga
    28081 aaatctataa ccaatattga aaatacaatt ttacaaacac caagtggtgc aattattaga
    28141 gttggagata ttgctgatgt taaagaaatt tctgcaccag aaaaattaga acgatacaat
    28201 cgattgagtt ctttacaagt attggcatca actgttgggc gaccaactgg ttctgtaatg
    28261 gcagatatta aattagatat tgaaaaagcc aatttcccaa taggcacttc tgttgattat
    28321 ttaggcgaag agaaaaacca agctgattca tttggaagtt taggtattgc aatgggtata
    28381 ggtattttat tggtttattt aattatggtg gcattgtacg aaagtatgtt gtatccgttt
    28441 gtggtattat tctctattcc tgttgcctta attggtgcaa ttttagcttt ggctttaacg
    28501 atggaaagtt taagtatttt tggtatcatc ggttttatta tgttgatggg attggtagcc
    28561 aaaaatggta ttttgattgt cgattttacc aatcatatta aagcacaagg gtatggttta
    28621 aaagaatctt tgatagaagc aggtagagaa cgtttgcgac ctattttaat gactacgtta
    28681 gcgatggtat ttggtatgtt gccaattgca ctatctaatg gaccaggagc tgagtttaaa
    28741 aaatctatgg cgtgggtaat tattggaggc ttaaccagtt cattattact gaccttattt
    28801 gttgtgccaa cggtttatta tattttagaa actttgaaaa ataaaattca aggaagaaaa
    28861 ggcgaaaatg caccaaagaa aaagacgatt tctgaaatga tttctgaata ggattccgta
    28921 tctttatttt tagatggaat tcagttattt aaaagcccta cacattattt ttgtagtcaa
    28981 ttggtttgct ggtttatttt atatgcctcg tttatttatt tataccaaag aagcgagtga
    29041 aaaaaaagag ccagcacgtt caattcttct tgaacaaaac ctaataatgc aacaacgact
    29101 ttggaatatt ataacgtggc cttcagcaat tattacattg ttgttggcta cgcttatttt
    29161 aattcaaaat cctttttttt tacaacaacc ttggttgcat actaaattgg tttttgtggg
    29221 attgttgtat ggctatcata tttattgcca tattatttat aagcaacaac agcaacatat
    29281 tttcaaactc agttccaaac aattgagaat ttggaatgaa ctagcaacaa ttttactgtt
    29341 tattattgtt tttttggtgg tggtaaaatc acaaatgagt attttatttg caataatcgg
    29401 agtattttcc ttagctgttt tactaatggc aggaattaaa ttttacgata aaataaggaa
    29461 gaaataaatt attgcaatta aaaaattcac atcttaaaat gtacagcaat actatatttt
    29521 aaaaatattt ttattaggat tttttatgct tatattgtaa ttaattatga atttgtttta
    29581 caaaaatttt tataggtatt attggttaat ttcattattt acactttatt tattttttag
    29641 aaattaccaa acttttttag acgtttcatt attagacgaa agtggttatt tattgttatc
    29701 aagatttaac cctttaattt atcctggtgg ctatggtgca ttatatattt tatgttataa
    29761 aatattacat cagttgatta gcaattctat gcaattacat ttttttgcta ttatttttct
    29821 tacatggttg ccttctataa ttgtttttta ttttttattt tttagaaaag ttccagcttt
    29881 tttatgtgta tttatttctt ggacattgat gctctcgcct tatattgcag catttgattg
    29941 gtggcctaga attagtcatt ttgcaatttc ctttattttt atttttacga ttattagttt
    30001 taagtatcat ttcaataagt ataaattcct tttatttgga attttagttt gtggtattct
    30061 ttcttatgca cgacctgaat taatgttgag tgtttatatg ttactgggtg ttttaatagc
    30121 tatattttct tatgaaagga ttgtacaaaa aaaacaatcc aatgttggtt ttacaaagcg
    30181 taataaaata cttttttcca ttatgacatt tgttttctta gcctttcttt tagtatggaa
    30241 aaatcctcta aaaaatacag gaagagctta ttttgctcta ggacaacatt atacttttaa
    30301 tgtattaaga tgggaaaata aagatatgac ctattttatt cgatggcctg aagtctttga
    30361 aaaaaaattt ggaaaaagta gaacttttaa gcagatgtat aaatacaatc ctagtgaaac
    30421 gaaaagacat gttgtagaaa atattaaaca ttattttgaa cagacttatc gatttttacc
    30481 agagctttgg atgccaagta ttctttttaa aatatcagca actattaagt atatagtatt
    30541 ttttttaatt tatttggtct gcttgtttta tttaggatta aatacttact ggcaacagtt
    30601 aaaacaaaag gtaattgata atttttttta tgtgttgttt tttttaataa tagcaacgcc
    30661 ctcattattc gcatcattta ttatttatcc gagagagcat tattttatta tgcaattgcc
    30721 attgtttctc tttttaattt atttattgat agagccattt ttttcattaa aagtaaaacc
    30781 atcactttct aaagtaaccc tagtaattac gatattcata attttaattt taacgccaaa
    30841 tatttcagct tatcctcgat tcaataagtt tgatagatat gaacaaccaa actatgttcc
    30901 ttatataaaa tacttagaaa gtttgaaaat tgacaaaaaa gttaatttct taactatgga
    30961 gtttttacaa atttatttac ccaataatta tgcttatgca actgaatttt tcactaaaaa
    31021 gccattttat gattcattaa ttgttgcaaa agatattaat ttaatttatt tatcacccta
    31081 tatggagaat gaacctagac atcaatctga cagtactttt aagtatttta tgaataatta
    31141 ttcaagatta ggttggaata aagtccaatt aaaagacaaa acaggttatt tattaataaa
    31201 gcaggaactc ttgggtctgc attcactaat tatagagtaa aataaattaa tagcctttaa
    31261 gcggaattgt atattgttta ttttgtagag gtagtctaaa ggctacctca attgattttt
    31321 ttaatattct gaatatcaat agccttatca atattttgaa tatcaatagc catcagcttt
    31381 agctgtggct actgtggcca ttaaaaaatc aagcagtttt attcggtttg tattattttt
    31441 tttgttatta ttgtaaggta tttaagttta cataaaaatc acataattaa aatgaaagat
    31501 atatttaaaa ttttatttat agtatgtata agcacgtttt atagcagtag ttatggacag
    31561 tcgtgcttta aaaataatta ttctgggttt tcaaatacaa ctaattattt agatgtagat
    31621 acaactaatt ttatagtaac aaatggaggt agccagttag aaaactatca tttatcttct
    31681 ttaaaattag caatagaaat tggtaaaatt gcagatacat tggggaatga aatatcttgt
    31741 agttctgctg gatttcacca aaatacacca aattaatatt atgaacaaat ttacagcgta
    31801 tagagttgaa tatacacctg aattatctgt tgttactggt ttagataaaa taaaatttaa
    31861 attaagagca ttagttatta agccaaataa tgcaagcaat gcgccttgtg ttttagttac
    31921 acatggaaat gacggtgtgt tgagagattg gagaggtaat ttgctttatg gcgtttctga
    31981 ccttttaatg aaaggctatg tagtagtaat ttttgaaaac ttatctacgc aaaggcgagt
    32041 gccacgttct gatatttggg gttgtatctc aaaaacctat aaatatgtat ctgcaccagg
    32101 aacacctatt tgtatgtcaa ctatgctttt tcatattgca tatataaatt gtggaggaca
    32161 acagagtgga agtagatgga aactttataa ttttctaacc attgatgcct tagctaaata
    32221 tattgcccaa aatagcagta catataatgt taatccaaat aaattattta cttatggttt
    32281 tagttttggt tctggagttg cggaaggttt tctaactcaa aaaacggaat ttccaagtgc
    32341 tttgctatat aacacgagtg ttaatcatac gccgattaga caggcaggct ttaattcaac
    32401 agttggcgtt aattttacaa tttcaagtgc agacatttct aaatatacaa atttaggcgg
    32461 tattccaatg agtagtcctt taatgaatgc agataataat tgtggtaaag ttgatgttta
    32521 tacagcagat gactctacta aaagattatt attaattcat ggatttgctg atagtgctac
    32581 tactggtagt atttatttta atgtttatcc tcgtaaacaa gaaatattag atagcgtaaa
    32641 tataaaaaac cacttaatca cagtttgcgg agaaggacat tctattttta atggttttga
    32701 taattttatt tcagctactg gaaatatatt taaaactaca gatcgtaatt atccttttta
    32761 ttcatttgta aaaggtttaa ctatttctaa tattaataca atgaaagact cgctaaatct
    32821 gaatactact tataaaaact ttatggatac gataagaggt atagataatc aaatttttca
    32881 attgtataaa tcaggaagcc agttttttgt taatgctata aataatactt cgtatatttt
    32941 gtgttctaca gaaaacagtt atgatagaaa aacatatata gggaatgatg gaatatatgg
    33001 aagtacttat aatgcaaatg attggttgta taaaatatac aatgcgagtt gtgaactgcc
    33061 aagcggagaa agaaaagcaa aagagatgaa tagtattaaa gaaatgggct ttaatatacc
    33121 tatgttgtat ccaaatccga gtaatggtat ttttaatatt gatatacccg ttatatttaa
    33181 tataaaagaa ctaactttag taatttataa ttcaaatgga caagttgtta gttctcaaat
    33241 aataaataaa acataccaaa ctggcgaaat tataaaagaa caatttgata tatcaaatca
    33301 atctaacgga aattattata tacaaatcag aagtgataca agaatattat tgagtcagat
    33361 tgtcatatta aaaatttaat tttttattga tttaggttat taatctttca aaaaaaggaa
    33421 aatgcagccg tactttcaaa ctaataatac aaatattact caatttgata gtatacgatt
    33481 tgttatagca atatttgtaa ttattgtaca tcatcagttt actaatataa atattccttt
    33541 tgcatttgca tatatggcga tgcatatttt ttttatcatg tctgcttttc tgatttcgag
    33601 aggcttgttg ttaagcaaat ctaaggcaca aacatttagt ttgttcttta aagattttta
    33661 taaaaaaaga attttaagaa tatttccggt atattttgct tatctgtttt ttctaatcgg
    33721 aatatctatt gcgttatatt tattaaaatt tggagacata ttaaaaataa ttactgaatt
    33781 aaagaagttt ggatggatgc tttttacatt tacttataat tatagagaat ttttacttca
    33841 tataaaaaat atgccattga tagaatcttt ctttttttct catctttggt cgctttctat
    33901 ggaagaacaa ttttatattg taattccttt tgtaatttat tttatgaata gaaaacaatt
    33961 agtttggtta tctgtaattg caattattat tacaccaatt tttagagttt ggtattatga
    34021 gaatgtatta gtgaatgaag aagattatat attaagagga ttgatttatt acagagcaac
    34081 ctttttacaa tttgattgtt ttttttatgg tattttgtta gcgacctata attttaaaga
    34141 ttttattaaa acatataaga taatatttta tttactgctt attgtatatt tatttgttat
    34201 tatttataat ggatttatca tttcaaaaga aatgaatttg agctttttta gttcaattat
    34261 taggtacgat tttatgatat tgaataagca atatctttat catgatgtat taataaattg
    34321 gctgatattt tttatgtttt taaattgttt ttataatcca aattttttat cctttctaaa
    34381 ttttaaattc atattgaatg ccggaaataa gtttacttat agttcttatg tttatcaata
    34441 tttatttatt ttacctagcg taggcatttt atacccttta ttaacaaaag ttcttggact
    34501 aacagaagta ttggcacaat taatagcact aattgtttca tctgttggaa tttattattt
    34561 atcttcattt agttataaaa aaatggaaat aaagtttcta aatttactaa aaaaagaaaa
    34621 acatgcaaaa tagtgctacg cagaacattg aaattaaaaa accttctgtt cagtattttg
    34681 aagtaataga tggtttgcga ggttcttcta tgttgatgac tattatgaat cacatgtttt
    34741 ttttgcaaat tttaaaaatt cctgttgttt ttgctcattt tggtttgcat ggctttttta
    34801 tattatctgc ttttttaatt agcagtattt tatataaaga aaaagaaaag tttggtgtat
    34861 ataaacctta tgttaaaaac ttctatataa aaagaatatt acgaattttt ccagtctatt
    34921 ttctctatct ttttgcagta ttaatgctgg gtttagctac aaaaggcaca ccattacaag
    34981 cacctttggg cattatttat gatttgaaac attacggctg gatgttgctc acttttacgt
    35041 tcaattatag agaattgtat tcctggttaa tcaatgaaag ccatttgcgt tgtatgtttt
    35101 ttccgcattt gtggtctata tcattagagg aacaatttta tttggttgca ccaaccttag
    35161 tgttcttttt tagaaaagaa acaattaaga aaattgctat tgcatcaatt ataatctatc
    35221 ctttttttag gttttttggc tatttatatt tgctaaacga tgtaaaaatg atttctccat
    35281 ttactgtaga tggacacgat gaattaagtg cattatatga tttcttttat cgttctagtt
    35341 tatttcaatt tgatgcattt atgtatggca ttttaattcc cttggttagc tatgataatc
    35401 gtaaagtttt aagatggata gtaattattt cgttttttac actattagca agtcagttat
    35461 ataatattat agatttagct cacgatagtg gcagaccaat tttgcaagtt gccaaccatg
    35521 cagatgtcat tatgagaaat ggacaatttg gttatatcaa tacattatac aatattttag
    35581 gagcctcttt gttttattat ttattaaaat ttcctgattc ttctattcaa aagccgcttc
    35641 gtttttcttt ctttaaaaat tgtggtcgaa ttgtttatgg tatttatgtt tatcacccaa
    35701 tttttattat gattactttg gtattgtata acttgttttt tagtatgatt tctaaagaag
    35761 ttggagatat gtttattgta aaattattag cagatattgg agctgttgca ttttgtttta
    35821 caactaccta ttatgtatgt aaactgagtt tctataaatt tgaaatgcct ttcttattat
    35881 taaaagccaa acgttcagag cagaaaaaga tttaagaatg agatgtgaga tataagagat
    35941 gaccgtgcct tcatttcgac gataggataa atatctttta gatttgcaga aacccaaaac
    36001 aaattttagc tctgctgaat tttcctttct cgctttgctc gaaatgaaaa aaaatgaaac
    36061 aaccaataat aatttactat cgactaataa ctaaaaaatg gattttgaga gattaacttt
    36121 tagcatacat caaactaacg attttttact aaagaatgca gtaaaggcag taaatgttaa
    36181 tattactctt agaaattggc taattggttt ctatattgta gaatttgaac aaaagggaag
    36241 tgacagagcg aaatacggaa ctaagttgct agaaaatttg gcaaaaaaca tcagcattaa
    36301 aggactttct gcacctgaat tatcacgttg ccgacagttt tacaatactt atcctcaaat
    36361 tcttgcgaca gtaacccaaa aattcaaaac cttagttcct actcatttta ttgaagattt
    36421 tgtacataag tcaattcttg ggatagcatc ccaagaatta gataataata taataagtca
    36481 atcaacaact gaccaatctc aaaacgaaca tttacatcaa ctcttgtctt cctcatcgta
    36541 ttcacacctt gttgaactca taaaaataga ggatagcacc aaaaggcgat tttatgaatt
    36601 actaatttta aagacacaac catctgtaaa agaactcaaa agacaaattg atacacttac
    36661 ttttgaaaga gtagggttat cgggtaatta tgaaaaatca ttcgataaaa taataaagaa
    36721 gataacacca caacaaacaa atgatttggt taagtcgcat tatttttttg agtttttaaa
    36781 tatagaacag ccacatttga tagaagaaag tgcattagag caagcactta ttcaaaattt
    36841 aaaacagttt attacagaac ttggtaatgg gttttgcttt gaagcccgac aaaaacgtat
    36901 attgattggc gatgaatatt attttataga tttagttttt tatcatcgta ttttaaaatg
    36961 tcatattttg atagaattaa aaacagaaat agccaaacat gaacatatag gacaattgaa
    37021 aacgtatcta aattattaca aaaaaaatat tcaagaagta aatgacaatc cacctgtcgg
    37081 aattttgctg gttaccaatc aaaacaaggc attggtggaa tatgctattg ctgacagcga
    37141 tttagatatt tttgtaagta agtaccagct tcaattacca gataaagtag aattggaaac
    37201 atttattaat aacgaattaa aaaatagata aaaagtgata agggaagaca agaattattg
    37261 tgtatcaatc aataaaccaa acagcgaacg tcatttcgac gttaggaaga atttcttttt
    37321 agcatgatga aacttgaaac agattcagct ccgctgaatt gctgtttatt ctgatggtaa
    37381 attcgataaa aatcaaaatt ttgaaaatga gtgaagaaca aaaagtgcat aaagaacaaa
    37441 ccacaagaag tattggctac atcgaatcta ttgatggtat cagaggtttt tatgtgtttc
    37501 ctataattat tatgcacttg catttgcaac acataaaaat gccggctttt attccgcatt
    37561 ttacgttgca tttatttttt attctgtctg cctatttaat cagtagtatt ttatacaagc
    37621 aaaaagaaaa atcggagtct tttaaattat ttgccaaaga tttttatatt aagagagcct
    37681 tgcgaatttt gccattatat tgggcatata ttttattctt ttttggagta gcagtagttt
    37741 tgcgtacagt agcaccggca ttagaacaaa attttctcgg cgtaattcac gatttaaaac
    37801 attatggttg gatgttactc acgttcacct tcaattacag agaattattt tcgtggtctc
    37861 aggatttgag tcacttgcgt tgtcacgtat ttccgcactt gtggtcaatt tctattgaag
    37921 aacaatttta tttgataata cctacactaa tattttttct gaataagaaa agcataaaaa
    37981 ttatatcaat aattgtagtt tgtgtgtatc cactgattcg cacattcgga tatatttatt
    38041 ttcgtgatgt tgtagaaatg agaactactt atggtgacga atggaatgct ttatttaatt
    38101 tcttctatcg ctccagtttt tttcagtttg atgctttctt ttatggactt ttaatcccat
    38161 taattcgtat tgagaaaaga tatatattgt atgccggtta ttttatttct ttattattga
    38221 tgatactttc tcagtcgtat aatgctgtat taattgctga tgagcagaaa atcagttttt
    38281 ggtcagcaat tcatttacca acagttattt acagcaataa tcagtttatg tatatgaata
    38341 ctttgtataa tattttcgga tttttcctta tatatataat gataaacgac ccaaaatctt
    38401 tattgaataa attttataat gcaaaagtat tcagaccaat aggtaaagta atttatggta
    38461 tgtatgttta tcatccagtt tttgcattgt taacgttagc actttattat ggctttttag
    38521 taaaagtaat tccgaatatt ttagcagata taatagctat tgcaatctgt acaacagcta
    38581 cttattattt cgccaaatta agctattata aatttgaaat ctatttcttg aacctgaaat
    38641 ctaaaaagta gtttttcaaa aaatataaaa taaaaaaacc acagaaatgc tgtggtttta
    38701 atttaaaatc aaatctgata ttgtatcagt ctctgaaata tggagcggag agagaggga
//
LOCUS       DAOQCW010000007        37024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_389_length_37024_cov_5.838351, whole genome shotgun sequence.
ACCESSION   DAOQCW010000007 DAOQCW010000000
VERSION     DAOQCW010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 37024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37024
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_389_length_37024_cov_5.838351"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..2264)
                     /locus_tag="PLS16_01380"
     CDS             complement(213..2264)
                     /locus_tag="PLS16_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNJ59758.1"
                     /translation="MAQNFSGKYPKTKKGKQKDIYFGTKVADPYRWLEDDLSADTKKW
                     IKEENAVTFDYLEKIPFRDALKKQLTDLWNYEKISAPFKKGTYSYFYKNDGLQQQSVL
                     YRISPEGNEEVFINPNEFSKDGTTSLADVSFSKDGSLCAYQISEGGSDWRKVIVINTD
                     NKKQIGDTLFDVKFSGISWKMNEGFYYSSYDKPKGSELSEKTDKHKLFFHQLGTTQKE
                     DKLIFGGEQTPRRYIGGGVSEDGRFLIISTAVSTTGNELYIQDLSVKNAPIIPIVSDF
                     KNSTDFVYNIGDDLFLLTNFNAPNNRLVKVNLKNLASDNWVDVIPETENVLGVSTSGG
                     YFFAQYMLDAIDVVIQFDTKGNKIRNIELPGKGSVAGFSGKQEDTTLYYTYSNYVTPS
                     TIFEYNIETGKSLMYKTPKIKFNPEDYISEQIFYTSFDGTKIPMMISYKKGIVLDGKN
                     PTILYGYGGFNVSLTPSFSVSNAVWMENGGIFAVPNLRGGGEYGQKWHDGGRKLNKLN
                     VFKDFIAAADYLKNNKYTSTDYLALSGGSNGGLLVGATITLQPDIAKVALPAVGVLDM
                     LRYHKFTAGAGWAYDYGTAEDSKEMFDYLKAYSPIHNVNKGVSYPATLITTGDHDDRV
                     VPAHSYKFAAALQSKQVSTNPVLIRIDTKAGHGAGKSTEAYINEACDRMSFALFNMGR
                     K"
     gene            2469..2858
                     /locus_tag="PLS16_01385"
     CDS             2469..2858
                     /locus_tag="PLS16_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HNJ59759.1"
                     /translation="MNSIKMPDIKDINDIETIILDFYEKVKVDKLLRPFFFGEKPTKW
                     EKHIPLMISFWENVLFYSGNYEGDPLLIHRKINKIKATETKHFERWNNLFDETVNAHF
                     KGANAEKMKQHAQAIAEIMLSKIEPSN"
     gene            2945..3841
                     /locus_tag="PLS16_01390"
     CDS             2945..3841
                     /locus_tag="PLS16_01390"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HNJ59760.1"
                     /translation="MIRPRFLQIGDTIGITCPARKVALEEIQIAIQILESWGLKVVLG
                     STIGKANHQFGGTDKERLDDLQNMLNNPDIRAIIAARGGYGTVRIIDQLNFNTFMEQP
                     KWLIGFSDFTYLHTVLNSNIGIETIHGIMPITFEKSTPEALESLRKCLFGESLEYHFS
                     SHKLNRPGTIEGEIVGGNLSILYSLLGTKTIVNTSNSILFIEDLDEYLYHIDRMMMAL
                     KRANKLSNLRGLIVGGMTEMKDNTIPYGKTAEEIILEHVAEYNYPVCFDFPAGHIEDN
                     RSILMGKKAKLFVQKDKCIFNQ"
     gene            complement(3846..4304)
                     /locus_tag="PLS16_01395"
     CDS             complement(3846..4304)
                     /locus_tag="PLS16_01395"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNJ59761.1"
                     /translation="MLSKIQQEAKEVLTNYIEKNQLRKTPERFSILEMIYDTEGHFDA
                     EQLYINIKNKSINVSRATVYNTLDVLVACELVTKHQFGSNHAVYEKAYGSRQHDHLIC
                     MECGEVFEFCDPRLHQIKQTAAELFDQKVRNHALTLYGNCQKEKCPNKKK"
     gene            complement(4385..6193)
                     /gene="typA"
                     /locus_tag="PLS16_01400"
     CDS             complement(4385..6193)
                     /gene="typA"
                     /locus_tag="PLS16_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNJ59762.1"
                     /translation="MIQNLKVRNIAIIAHVDHGKTTLVDKILWECKTFSEHENTGVLI
                     LDNNELERERGITITAKNAAVKYKDYKINLIDTPGHADFGGEVERVLKMADGVLLLVD
                     AFEGPMPQTRFVLQKALQLGLKPIVVINKVDKENCTPDEVHEKVFDLMFQLDATEDQL
                     DFPTLYGSAKGGWMSLDHKRPTTDLVELMDAIIEHIPAPRIEEGAAQMQVTSLDYSSF
                     VGRIAIGRVFRGVLKTNMQVALIKRNGAISKSRIKELLLFEGMNKVKVDEVHAGEICA
                     IVGIEGFDIGDTIADAENPEAMPPIAIDEPTMSMLFTINDSPFFGKEGKFVTSRHIKD
                     RLTKELEKNLALRVQQGETADSFLVYGRGVLHLSVLIETMRREGYELQVGQPQVLFKE
                     IDGVKSEPIEELTIDLPETLAGTAIEMITKRKGDMKNMILKGNRSIMEFEIPSRGLIG
                     LRTEMITATSGQAVMAHRFIKYEKHKGDIQGRINGSMISKETGEAIAYSLDKLQDRGR
                     FFVEPGEEIYTGMVIGENSRQDDLVVNCSVTKKLTNVRASGTDDKVKLPPPVKFSLEE
                     ALEYIQGDEYVELTPKSIRIRKIYLDEGERKRKSGK"
     gene            complement(6488..6561)
                     /locus_tag="PLS16_01405"
     tRNA            complement(6488..6561)
                     /locus_tag="PLS16_01405"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6525..6527),aa:Ile,seq:cat)
     gene            complement(6663..7742)
                     /locus_tag="PLS16_01410"
     CDS             complement(6663..7742)
                     /locus_tag="PLS16_01410"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ionic transporter y4hA"
                     /protein_id="HNJ59763.1"
                     /translation="MKKIVKLYEWTTSLPIITCLLYFSGLLDNNVFFQVFAGILLLFC
                     VMSAVHHSEIVAHRVGEPYGTIILAVSITIIEVAIIISLMMTGGNEYASFARDTVYAA
                     VMLILNGIIGLSLFIGGRKHHTQTFSPHSVKIALVALVSIITFTLILPTFTSSIVGPY
                     YTERQLLFEIIACLVIYIAFISAQTIRHREYFITESTSNAENKHNNISNIAFIISLTF
                     LLICLVIVVLLAKSLSTPIESLLTSYGLPRSLVGIIIAAVILLPEGIAAVSSAKQNKL
                     QNSINLALGSALASIGLTIPSVSFVSYLFDLPLVLGLEILPIILLVISVFTVMLSLIG
                     GKSNFVYGVVLLVNLFAYVFLTINP"
     gene            complement(7815..8852)
                     /locus_tag="PLS16_01415"
     CDS             complement(7815..8852)
                     /locus_tag="PLS16_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59764.1"
                     /translation="MLKKSSFCILFCLLMSISSLFAQNASSINDTTKWANTPLKFVFA
                     TIKFPQQTNLEKKDIYTTNGLQTLYFFKSELTAIDLTLSVSMRQVSTKEQKKATQEEI
                     NRMAVQYGGYPNVYKEKDKNGRNYDYVLIHTLEGKAISSRIFYIDGYLVILSSVANQT
                     NKNQTVVDYFFDSFTPFSSINSNENNVAEKNKQASSEIPWAVFKEVNFTSKFPIEPNS
                     KTYVVQQADSSYYLIKNYYLQKGDKSLSYLVSERSYTYQLGITADSLFKTAFDALKLE
                     GKGKLKSETNLFGYKFPAKEYVFTSRKAYYRLRYFFANNALYQVMIAGNKKQVTDLKN
                     EQFFQSFQILQ"
     gene            complement(8883..10001)
                     /gene="dnaN"
                     /locus_tag="PLS16_01420"
     CDS             complement(8883..10001)
                     /gene="dnaN"
                     /locus_tag="PLS16_01420"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNJ59765.1"
                     /translation="MRFIISSAQLLKNLQKISGVISSNTVLPILEDFLFDINNGKLTV
                     TATDLDTTMIVEMEVESKENFKIAIPAKILLDSLKALPEQPITIAVDENTNAIEITTD
                     NGKYKLSGENSADFPKEPVADEVEQVSVASNVLANGIQKTIFAVSNDELRIAMTGVFF
                     TIDENGMTLVSTDAHKLVKFNRSDITSSNPSSFIVPKKALNLLKGILANNDTAVSLQF
                     NKSNAFFVFDNAKLICRLVDARYPDYNAVIPKDNPNLLTINKDDLHAALRRTSIFSNK
                     TTHQVVLRLSGSELSVSAQDLDFSNEASEKLVCEYQGDPMEIGFNAKFLIEMLSTLDN
                     AEIVMELSTPNRAGIIKPTDKNENEDLMMLVMPVMINV"
     gene            complement(10122..11045)
                     /gene="miaA"
                     /locus_tag="PLS16_01425"
     CDS             complement(10122..11045)
                     /gene="miaA"
                     /locus_tag="PLS16_01425"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNJ59766.1"
                     /translation="MKTKIQKYLIVIAGPTAVGKTKVAIEIAKYFDTDIISADSRQIF
                     RELNIGVARPSEEELNTVKHYFIANKSIDEYVSAGTYECEVLELLNTLYQKKDIVVLC
                     GGTGFYTNAVLNGFDEMPKVNPEIRSELNAIFRNEGIVPLQEKLKILDIDSYNTLDIM
                     NPQRVIRALEICIGTGKKYSDFKTKKSIERNFMPIKIGLDLPKEILHKNINNRVDKMM
                     EEGFLDEAKSVYEHKKNNALQTVGYKELFDFLDHRTSMEEAVELIKLHTRQYAKRQMT
                     FFKKDKEIQWFAPNEIDNMLQFINIKRLEYF"
     gene            complement(11042..12076)
                     /locus_tag="PLS16_01430"
     CDS             complement(11042..12076)
                     /locus_tag="PLS16_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HNJ59767.1"
                     /translation="MFKQQHILAILKCLTIGKLYNMLLVYTSFYLTKYLKKPIQWGLP
                     FSISIEPTTSCNLRCPECPSGLRAFTRNTGMLEPAFFEKTIQEIKQHLLYLTFYFQGE
                     PYLNRDFLKMVKVAKNNKIYTSTSTNGHYLTDAVAKETVVSGLDRLIISIDGTTQETY
                     QQYRVGGHLDKVLEGTKNIIKWKKALKSTSPYVIFQFLVVRHNEHQIEDLKKIAKEMQ
                     VDEVILKTAQVYNYENGNDLIPLQEKYSRYKKLSNGKYALKNKLDDACWKLWHSNAVT
                     WDGKVVPCCFDKDAKHILGDLQKDSFQTIWHNQNYWNFRRQITKGRKEIDICRNCSEG
                     CKVNFNVEID"
     gene            12198..13280
                     /locus_tag="PLS16_01435"
     CDS             12198..13280
                     /locus_tag="PLS16_01435"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ59768.1"
                     /translation="MNSKQKILIAFLDWGLGHATRCMPIVDALLERNYQVIIAGNGKS
                     LLMMQEQYPNLKSYELPDYNIQYNGDKNAAWQILFQARKIYNAIKKEHQFLSTILEIE
                     NINIIISDNRYGIYHQNCKNIFIGHQIAIQAPRPFKFVEPFLLKQILKLISKFNELWI
                     PDMEDEVHNLSGVLSHKIKFTIPVKYIGILSRFNKDNHSSKIDDKRYKIVALISGVEP
                     SRSDFEQKIIKSLEETNRTCLLICGRIEKQKSIKQHKNITIINFLQGDKLEHYLQQAE
                     VVICRSGYSTVMDLMALEKKAIFIPTPGQTEQEYLAENLAKQGIAIYQKQSSMNLVAA
                     LSQIESIKGFKNNTSFLFRKEVLDDL"
     gene            complement(13291..14106)
                     /locus_tag="PLS16_01440"
     CDS             complement(13291..14106)
                     /locus_tag="PLS16_01440"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HNJ59769.1"
                     /translation="MDKIQQIIEQAWENRTLLQEKSTLDAINEVIAKLDSGEFRVAEP
                     TENGWKVNDWIKKAVILYFPTQKMETIEVGPFEFYDKIPLKKNYEKLGVRVVPHAIAR
                     YGAFLQKGVVMMPSYVNIGAYVDSGTMVDTWATVGSCAQIGKNVHLSGGVGIGGVLEP
                     VQAAPVIIEDNCFIGSRCIVVEGVRVEKEAVLGANVVLTMSTKIIDVSGTEPKEYRGV
                     VPARSVVIPGTIPKEFPAGTFQVPCALIIGQRKESTDTKTSLNDALRDFGVSV"
     gene            14175..14792
                     /locus_tag="PLS16_01445"
     CDS             14175..14792
                     /locus_tag="PLS16_01445"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59770.1"
                     /translation="MHFRIYILLFTVLLFSACKTRTVEEKDLNGNILKKYSVLKKDVS
                     KKHGAYTAYYDSGELLENANYKDGKLDGERILYFQNGKIMSKEHYNMDNMEGVYENYY
                     ENGNIKQKGIYKNNQIADTWTNYYEQPANQIKESFTIKDGMINGLYKEYATNGTLIIE
                     GNKVEVEVGLDFFDGKVTMFDSISKKPVMVFEFNKGKMIKKDSII"
     gene            14805..15215
                     /locus_tag="PLS16_01450"
     CDS             14805..15215
                     /locus_tag="PLS16_01450"
                     /inference="COORDINATES: protein motif:HMM:NF027592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HNJ59771.1"
                     /translation="MKKVNLLLLSLILFLATACKMPEPKELGVKFEENNPISIDDLIA
                     QLQTTPLIKAVQLKGEINKSCLSEGCWFTIKDKNGESHTIDVKDKKFRLPNSSAGKTV
                     VALLDAKIDTTQDEPEIILETIGIKYLDENSSTQ"
     gene            complement(15204..15887)
                     /locus_tag="PLS16_01455"
     CDS             complement(15204..15887)
                     /locus_tag="PLS16_01455"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphatase"
                     /protein_id="HNJ59772.1"
                     /translation="MHSHLLPGIDDGVKSIEESIEIIRHFIGLGYKKIITTPHIMKGT
                     YDNTSEIIKNKLQIVRQEIQSQNLDIEIEASAEYYLDTHFLDLLFNGDILSFGKENYV
                     LVEFSYLQKQNDYQNIFMNISQTDYQPILAHPERYAYFYDKNLSQLKQIKEQGVLFQL
                     NLFSILGVYGKGAKMVAEHLVKENMIDFVGTDIHKPKHIAYLDDCLKDKYLQKLLQSG
                     NLKNKSLLS"
     gene            complement(15972..18368)
                     /locus_tag="PLS16_01460"
     CDS             complement(15972..18368)
                     /locus_tag="PLS16_01460"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNJ59773.1"
                     /translation="MEKEPLFSNEKFSDEDINVRLILDQYLRYWPLYLVFIIIFSVGA
                     YLYLKYSIPVYQATSSILIEKENQSLMVDFSSIEDLFGGNKVVEDQMLVVKSLPVLMK
                     VVDNLLLDVKVTRKTKYTQKTVGVYDNRPFIIERKYLNGDIKDPLVFKIEIIDLENYQ
                     LTYNEKTYKFPFGKPLQIPELVSFKLILKKDLPQAVEGEEYTITLNPITATLLNLQEN
                     LVVEQAIKSSKVSNAINLSIESSNENKSKDILNNVVQEYFNNDILSKKIVSEKTYAFI
                     QDRLGVISTELSGVESDLEKYKTTNKVYSIVDESRLFADNSMSTKQALTDAELQLSII
                     NLMLGELNTNKNEILPVNIGLADVNIAVNIKAYNDLVLQDMQYKSSVSELNPERIALS
                     KSISSLRENIKASLNNLRVNNQMLVNNLRKQDNILEGELSLIPNKERVLRDISRQQQT
                     KEALFLFLMQKREEVAISTISIKPDAKIVNPAFSSGVPISPQKGKIILIFLVLGLIIP
                     TVIIYVLSLLDTKIHNLSELERLSPIPVLSSIVKTRKPETKILEKDDRRGIVESFRVL
                     VTNLVFSLSHISDSKSKTIMLTSTIAGEGKTFISSNLAAFLAYSNNKVVLLGMDFRAP
                     KLNLYFKNVKNKGITHFIKEENLEIEDIVNSTNVEGLDVIYPGLIAPVFLDITKSDRL
                     RSMMDQLKVRYDYIIIDSAPVGLVSETLSLFPFVDMCLYIVRADHLDKRALKISEKLY
                     NEGRFADFRLVLNAEDIRKSEYGYGNYGKYGGYGAYGDFEQEEKYKIWQYKYWKDLFS
                     "
     gene            complement(18374..19198)
                     /locus_tag="PLS16_01465"
     CDS             complement(18374..19198)
                     /locus_tag="PLS16_01465"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNJ59774.1"
                     /translation="MDKFNETTMNKNYYLKKNKLLYLLLIILITSCASPKEILYFQDF
                     EAGKERDISQKYTTKLQPDDVLNIIISSKDNLGTAPFNITSMAGTVGNSNITTQPKLM
                     NYIIRQDSTIEFPVLGKIEIAGKTILETIEYFKEQLKPYINEPVVVIEWLNFKYTVLG
                     EVRVPGLYRSTSERVSILDALGTAGDLTIYGRRDNIMLVRETNGKQKYYKIDLTSKNL
                     FESEVFYLKQNDVIIVSPNKAQIQSSAFNRNTPIFVSIASLLVSTITGVFLLVRKK"
     gene            complement(19404..21719)
                     /locus_tag="PLS16_01470"
     CDS             complement(19404..21719)
                     /locus_tag="PLS16_01470"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNJ59775.1"
                     /translation="MNSLFYVVPFLGIFGLVITAIKFAWVKKQDAGDSNMKELSGYIA
                     DGAIAFLKAEWKVLSYYGIIAAILLAFLGWKGENSSPVIAIAFLIGAIFSAFAGYVGM
                     KVATLANVRTTQAARTSLAQALKVSFTGGTVMGLGVAGLAVFGLGGLFIVFFFMFTDG
                     GAAANSHGLLKAIEVLTGFSLGAESIALFARVGGGIYTKAADVGADLVGKVEAGIPED
                     DVRNPATIADNVGDNVGDVAGMGADLFGSYVATVLATMVLGQETISNDNFGGLAPILL
                     PMAIAGVGIIFSWLGTFFVKVSNENGDVQGALNLGNWGAIVLTGIASFFLVKYLLPTE
                     MVLRGNTFTSTGVFGAIIVGLVVGALMSIITEYYTAMGKRPVLSIVRQSSTGHATNII
                     GGLAVGMESTVLPILVLAAGIFGSFLCAGLYGVAIAAAGMMATTAMQLAIDAFGPIAD
                     NAGGIAEMSQLPEEVRGRTDILDAVGNTTAATGKGFAIASAALTSLALFAAFVGVSGI
                     EAIDIYKAPVLAGLFVGGMIPFIFSALAISAVGRAAMDMVNEVRRQFREIPGIMEYKA
                     KPEYEKCVAISTQASIREMILPGAIALVTPILVGFLFGPEVLGGVLAGVTVSGVLMGM
                     FQSNAGGAWDNAKKSFEKGVDINGEIYYKKSEPHKAAVTGDTVGDPFKDTSGPSMNIL
                     IKLMSIVSLVIAPYITVGGLLNVDGTQGASCHTEMVETSELHSEKAACCSADAEAVTE
                     KESQELTEEVTNTTNNNEVIEASKDAATINH"
     gene            complement(21863..>23170)
                     /locus_tag="PLS16_01475"
     CDS             complement(21863..>23170)
                     /locus_tag="PLS16_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59776.1"
                     /translation="LFSFLFFASIGMVKAQDCPRHIDGDSLKTAPVYFYYSQYIKKKD
                     FEKAYPFWRTLYDKAPGLSSIIFYDGEEILKDKIEKTKDNPALQASYQDTLIQLYDKW
                     VKCHGNDDYVLGTKKAQAQIQYKNDIEGAKKSLERSLELSDKYPIVMQTYFNLLNYQY
                     QNGQISGDSITAKYDVLMAKLDKNIAKNGQYVNSYKDAKTAIENAYIQNFADKSNPEV
                     CAKLLAIYTKQYNANPNDPSTVQNVYEKTRGCADSALNVELLQKLNQLKPSYSYAVRL
                     ANIYIKANKLDDAYLLYENAIAHEPDASNKSDLYLLLANMKADKDLFPESRELAKKAI
                     ELDSTNARAYFLIGYLYASSGKLCGPGVGFQSQIVLWPAFDYFRKAVEFGDEEIKTEA
                     NKMLSMYQQYLPTKADITAKKLTVGAPYTVKCWIEEETTVQIK"
     assembly_gap    23171..23270
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23271..24521)
                     /locus_tag="PLS16_01480"
     CDS             complement(<23271..24521)
                     /locus_tag="PLS16_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59777.1"
                     /translation="MHKKIIFLLLQLIIVNNIITAQESSVYSRYGLGIMANHNNTATN
                     MMGGLGTAYRSLEGQSYINPASLSAVSLVSFDIGISGTFQNLKTTNLKAKQNSFNLDY
                     LSIAVPVKKFWSMSAGLMPFSKKDYEVTKYNQLDSSSSYLLETNGNGSLNQVFWNNGF
                     KIKDVSVGLGLGYIFGRIDNISATTTQTNGVADLTSYTSYQKGTYKAKALNVDLGVQY
                     QLNLKNKKDTTSPYRMVFGFAVNTPFFLNGQQYSNQLKSFYNEILVGRASDETLSDFI
                     NDKVENASYLQQFYSGKDWTTFAPDTFKTQTNHLSGMKAIPAYNIGIVFSRDYKFKVG
                     IDFRYQMWKNFKGFEQNTTADLNNSWRIGIGGEILPNIKKYNKLFSKLKYRAGFYYSK
                     TNISIRNNDINEFGIDFGVGIPMFN"
     gene            complement(24514..25233)
                     /locus_tag="PLS16_01485"
     CDS             complement(24514..25233)
                     /locus_tag="PLS16_01485"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNJ59778.1"
                     /translation="MNLTIDIGNTRTKVGVFSEMQLVEDWSVDRLSIVTVRKIIKQYK
                     IQYSIVSSTKLISDKLLEVLKSQTINIVLGEQTKLPFKNNYTTPNTLGYDRIALIAGI
                     QAKYPSQNNLVIGCGTCITYNFINKKNEFEGGSIHPGLKMRLKAMHTFTKKLPLIDLN
                     KPKNFVGKSTEDNLTVGTSLATALEIDAMINHYHVKFSKLNTIITGGDAVFLVSLLKN
                     KIFVHPNLTLVGLNHILEYNA"
     gene            25444..25938
                     /locus_tag="PLS16_01490"
     CDS             25444..25938
                     /locus_tag="PLS16_01490"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNJ59779.1"
                     /translation="MSISHSLLSELKHEAESTKNLLAVVPLEKGDWKPHEKSMSLSTL
                     TIHVAELFAWIADVVQETELDFANYQHPPKINTTEELLNYFNNAVQKSENALQNIDDA
                     HFFENWTLRKGDQVFFALPRVAAIRSFVLNHIYHHRGQLTVYLRLLDVKLPGIYGPSA
                     DSVM"
     gene            25942..26391
                     /gene="ybeY"
                     /locus_tag="PLS16_01495"
     CDS             25942..26391
                     /gene="ybeY"
                     /locus_tag="PLS16_01495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNJ59780.1"
                     /translation="MAINFYFDEVQKPKDFKTRRFKVWLSSIEKYYQTKIDDLSYIFV
                     NDEQLLAINQKYLAHDTYTDILTFDLSEIEKHINGEIYISLDRILDNAQKFNVPILDE
                     LLRVIAHGLLHLIGFDDKTKTKKSKMTEQENICIQLYHSLLIEKNNP"
     gene            complement(26388..27488)
                     /locus_tag="PLS16_01500"
     CDS             complement(26388..27488)
                     /locus_tag="PLS16_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNJ59781.1"
                     /translation="MRIGVNARFLLKDKMEGIGVFTDEICKRLVKLMPEHTFYFYFDR
                     DFDLSFIYSENIIGKKVFPPTRHPILWKWWFDYVLPKQMEKDKIDIFLSTDGYCSLNT
                     NIPQILTIHDIAFEHFPEYIPFLHAKFMRYYTPKYIEKAAKIITVSEFSKQDIIQQYK
                     LDSNKIEVVYNAFFEAQNDSQEYKFKINVPYFIFIGAIHPRKNVLNLIEAFEKFKQKT
                     SSEFKMVLIGRDAWHNEEFKTNLRKSSVQKDIIWIEKINRSSLIEYLKNAYALTYLSE
                     FEGFGIPLVEAMELGIPIIASNTSAIPEIAGNAAVYCEPNNIHQIAETMNNLVENKVL
                     YKQLKQAGLERKNLFSWEKSAEKIAKILLKYS"
     gene            27525..27830
                     /locus_tag="PLS16_01505"
     CDS             27525..27830
                     /locus_tag="PLS16_01505"
                     /inference="COORDINATES: protein motif:HMM:NF025479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HNJ59782.1"
                     /translation="MYLHNTTFKVALAHNNDFIYFIKDEIIPQYQEELELIDIVLLEL
                     LNVDMEDGKTYCLHIVFKDLQGYNEYKIYKEGKLLKAIFDKYNENVLFFSSILKKVD"
     gene            complement(27827..28834)
                     /gene="gap"
                     /locus_tag="PLS16_01510"
     CDS             complement(27827..28834)
                     /gene="gap"
                     /locus_tag="PLS16_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNJ59783.1"
                     /translation="MRRPRIAINGLGRIGRLVFQILFDKNLVDIVAINDIAPDAALVY
                     LLKYDTAQGKWDKEIKEIDDKFYIEGREILSTSFGNINQLPWKDLDIDIVIECSGRYR
                     TKDKLAEHIAAGAKKVIISAPADNEVKTIVLGINDNIISKDDLILSNASCTTNCLAPM
                     VKILQDNYGIQYGMMNTIHAYTQDQKLHDSIHPTDFRRARAAAQNIVPTTTNASKALE
                     WVMPEIKGKIIGGAVRVPVIAGSTTELYVQLEKQASIQEINEVFHKASKEKFKGILQY
                     TEDPIVSSDIIHNPYSCIFDAGLTQQNGGLTKIVGWYDNEYGYANRLAELVELVALKY
                     N"
     gene            28948..29022
                     /locus_tag="PLS16_01515"
     tRNA            28948..29022
                     /locus_tag="PLS16_01515"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28981..28983,aa:Glu,seq:ttc)
     gene            complement(29227..31185)
                     /locus_tag="PLS16_01520"
     CDS             complement(29227..31185)
                     /locus_tag="PLS16_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNJ59784.1"
                     /translation="MPENNKLDKLSIAGSLITLGIVYGDIGTSTLYTLKAIMGMSIEG
                     NHAVTEELILGGVSCIFWTLTIITSFKYIYLALNADNKGEGGIFALYALVRRYKAKWT
                     IVPALIGCAALMADGFVTPPISISSAVEGLTILYPELHTVPIVLVIIAFMFMIQRSGT
                     DTVGKLFGPIMFIWFVMIGVFGFINVIKMPFVLKAINPIYAFNLIFNYDGGFWFLGAV
                     LLCTTGAEALYSDLGHCGKSNIRVSWTFVKICLVLNYFGQASFILSHNNGEYNWLETA
                     PFYALMPREFLPYGIAIATAAAIIASQALISGAFTLVNEAMKLRLWPNMIVNYPTEEQ
                     GQIYIPSINWLLFFGCTAVVLVFRESSKMEAAYGLAITINMLMTTALLAYYLIIKRKS
                     KWIVYGLLILFISIEGSFFLSSILKFAHGGWFTVVIAIVIFGSMYLFNEGRKIRNRHL
                     EFVEMDDLLPQLIDLQNDITIPREATNLVYLVNSNDKRKIDSNIIYSLFRKKPKRADA
                     YWFVHVEITNEPYGAVYSVDTIIPEKCFFVKLQFGFKVEHKVHAMFMKIVEEMGKNGE
                     IDTLSHYPSLRKHNYPADFKFVLITSSVSADNELNAFNKLAMDAYAMIKSLSVPVEVD
                     FGLERTNVRTEKVPINIAKKSKIRLERL"
     gene            complement(31240..32421)
                     /locus_tag="PLS16_01525"
     CDS             complement(31240..32421)
                     /locus_tag="PLS16_01525"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HNJ59785.1"
                     /translation="MAKKTAQTKTSTTKKVVLPAKKAVANALVKKAAVPAKKVAVKTP
                     TKKALTPAKKAVAKAPAKKAVVPAKKVVVKTPTKKALTPAKKVVAKALVKKAVVPAKK
                     VVVKTPTKKALTPAKKVVAKALVKKAVVPAKKVAVKTPTKKALTPAKKAAVPTKKVTA
                     KVETSIVTTKKSATKVPVKKASVASKVTATKASTVKTPAKKVVATKPSTKIAPKKTET
                     KASSVNATATVKKVVVKTKLSALEKSRTKAHVSTQPTPTMTTETYVHKEKDLVKPQGR
                     LVRYNDEDLQEFKILIEGKLAKAQEEISFYQEQIKNAAENDTKSTGMEDGAFTSEKES
                     LNQTIARQQKLIAHLDNALSRIQNKTYGICRETGQLIPKDRLRAVPHATLCVEAKKRQ
                     K"
     gene            complement(32424..35768)
                     /gene="ileS"
                     /locus_tag="PLS16_01530"
     CDS             complement(32424..35768)
                     /gene="ileS"
                     /locus_tag="PLS16_01530"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNJ59786.1"
                     /translation="MSNTDKKFYKEYKQFNPTEIEQSINAFWKSNDIFKKSITQRNDS
                     PNFVFYEGPPSANGMPGIHHVMARAIKDIFCRFKTIQGYKVNRKAGWDTHGLPIELQV
                     EKSLGITKKDIGTKISVEDYNKECRKEVMKFKDKWDELTDKIGYWVDLDNPYITYENE
                     YIESVWWLISELYKKGYLYKGLKIQPYSPAAGTGLSSHELNQPGCYRDVKDTTAVACF
                     KLKADDEQLQNLFSSNQAIYCLAWTTTPWTLPSNTALAVGKNIDYVLVNTINPYSHQP
                     MQVILAKNLLGKYFSPENENANFSNYQNDGKNLPYSIVNEVKGSDLAGINYEQLLPFV
                     SNSKEKINGVAFKIILGDFVTTEDGTGIVHIAPSFGADDMKVANENGIGGLTLVDKEG
                     KFVDGTGEFSGRYVKDYKDDPNYISVDIDIAVKLKKENKAFKVEKHEHSYPHCWRTDK
                     PIIYYPLDSWFIKVTAAKDRLIELNKTINWKPESTGTGRFGQWLENLVDWNLSRTRFW
                     GTPLNIWRTEDGTEEKCISSIEELQTEIKQSIDKGFLPASFLEKAIDLHKPYVDEIIL
                     VSALGSKMYREADLIDVWFDSGAMPYAQIHYPFSGEHLSGVNFPADFIAEGVDQTRGW
                     FYTLHAIATMLFDSVAYKNVVSNGLVLDKNGNKMSKRLGNAVDPFITIAKYGADAVRW
                     YMVSNGQPWDNLKFDIDGVLETQRKLFSTLYNTYNFFAIYANVDAFAYKEQELVPYQE
                     RAEIDKWILSRLNSLIDEVAKALEDYEPTKAARAIEAFVVDDLSNWYVRLCRRRFWDT
                     EASNDKNAAFQTLFECMLVTAQLMSPIAPFFSDWLYNNLVGNESGSELSKIYESVHLV
                     YLPKVDTSKIDTTLEQRMDYAKNISSLVLSLRKKTKIKVRQPLQKILIPNIDIEFINQ
                     IDKVKDLILSEVNVKEIEYISDTSGIISKKVKPNFKLLGKKLGGKMKLVADEITQFGY
                     NEINTLEQNKKIDITVEGATYEILLEEVEIISDDIQGWLVANERGITVALDITITENL
                     KNEGFAREIVNKIQSDRKEQNFNVADKIQINVQNHLVLNNVINKFKDYICNETLCESI
                     DFSDNLADAKSFDINDETLLMTIKKI"
     gene            complement(35914..>37024)
                     /locus_tag="PLS16_01535"
     CDS             complement(35914..>37024)
                     /locus_tag="PLS16_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ59787.1"
                     /translation="IIQIFNAQDREFKKFEKINYEHYKANDKSVLYFSIFFPFVEIVL
                     AVSMALMLWFGSKIVLEETANVGIFAAFIMYLNMAFRPLRMLADKFNTLQMGVIAAER
                     VFKLLDNQEKNEDHGRIEKESFDGSIEFKNVSFAYKEDEYVLNNISFKIEAGKTLALV
                     GATGSGKSSITNLFNRFYEINKGEILIDDINIYDYSLENLRSAFAYVLQDVFLFSGSI
                     LDNITLKNPTIDKAKVIEAAKLVGAHDFIMDLPNGYDYNVQERGATLSVGQRQLLSFI
                     RALVYDPAILVLDEATSSVDTESELLIQQAIAKLIENRTAIVVAHRLSTIQNADKIIV
                     LQKGNIIEEGTHQTLMNTSNGYYKNLYEMQFANIH"
BASE COUNT        12329 a         6385 c         5002 g        13208 t
ORIGIN      
        1 ctaaaaaggc agcagcgaaa gcacctgcta aaaaagcagc agctccagct aaaaaagcag
       61 tagcaaaagc acctgcaaaa aaagcagcag ctccagctaa aaaagcacct gcgaagaaaa
      121 aataatttaa tctattctgc gcagaaaaaa agtccgataa tattatcggg ctttttttat
      181 tttaatattt actattcttt aatgatatca ttttattttc ttcccatatt aaataaagca
      241 aatgacattc tatcacaagc ttcatttata taagcttcag tagattttcc agcaccatgc
      301 cctgcttttg tatcaatacg aataagaact ggattggtac ttacttgctt ggattgtaat
      361 gcagcagcaa atttataact atgtgctggc actactctat catcatgatc gccagttgta
      421 attaatgttg caggatatga aacaccttta tttacattat gtataggcga ataagctttt
      481 aaataatcaa acatttcttt actatcttca gccgttccat aatcataagc ccaaccggct
      541 ccagcggtaa atttatggta acgcaacata tctaaaacac ctacagcagg caaagcaact
      601 tttgcaatat ctggttgtaa tgttatagta gctccaacta aaagtccacc attagaacca
      661 cccgaaagtg ctaaataatc tgttgatgtg tatttattat tttttaaata atcagcggct
      721 gcaataaaat ctttgaacac atttagtttg tttagttttc taccgccatc gtgccatttt
      781 tgtccatact ctcctccgcc acgtaagttt ggaactgcaa aaattccacc attttccatc
      841 caaactgcat tgcttacact aaagcttggt gtaagactca cattaaagcc accataacca
      901 tataatatag taggattttt tccatctaaa acaattcctt ttttatacga aatcatcatc
      961 ggaatctttg tgccatcaaa agatgtatag aatatttgtt ctgaaatata atcttctggg
     1021 ttgaatttaa tttttggagt tttatacatc aatgatttac ctgtctctat attgtattca
     1081 aaaatagttg atggcgtaac ataattactg taggtataat ataatgtcgt gtcttcttgc
     1141 tttcctgaaa aacccgctac agaacctttt ccaggcaact caatatttct aatcttattg
     1201 cctttagtat caaattgtat tacaacatca attgcatcta gcatatattg tgcaaagaaa
     1261 taaccaccag atgtcgaaac acctaaaaca ttttctgttt ctggaattac atccacccaa
     1321 ttgtcagaag caagattctt cagatttact tttaccaatc tattattggg tgcattaaaa
     1381 tttgtcaata aaaataaatc atcaccaata ttataaacaa agtcagtact atttttgaaa
     1441 tcacttacaa ttggaataat tggagcattt tttactgata aatcttgaat gtacaactca
     1501 ttacctgttg tagaaactgc tgtagaaata attaaaaatc ttccatcttc agaaacgcca
     1561 ccgccaatat atcttctcgg ggtttgttct cccccaaaaa ttaatttatc ctctttttga
     1621 gtcgttccta attgatgaaa gaataattta tgtttatccg ttttttcaga caattcactt
     1681 cctttgggct tatcgtaact agaataatag aaaccttcgt tcattttcca agatatacca
     1741 ctaaatttta catcaaataa ggtatctcca atctgttttt tattatcagt atttatgaca
     1801 ataactttac gccaatcgga accaccttca gaaatttgat acgcacaaag tgaaccatct
     1861 tttgaaaaac tcacatcggc taatgacgtt gttccatctt ttgaaaattc atttggatta
     1921 ataaaaacct cctcattccc ttctggtgaa attctataaa gtacagattg ttgttgcagt
     1981 ccatcatttt tataaaaata agaatatgta ccttttttaa atggtgctga aattttttca
     2041 taattccaca aatctgttag ttgctttttt aatgcatcac gaaatggaat tttttctaaa
     2101 taatcaaatg ttactgcatt ctcttctttt atccactttt ttgtatctgc agataagtca
     2161 tcttctagcc aacgataagg gtcagcaacc ttagttccaa aataaatatc tttttgtttt
     2221 ccttttttgg tttttgggta tttccctgaa aaattttgtg ccataagatt tgtacttatt
     2281 ataatgctaa ataaaaaaat acttaatatt ctcattttaa ttgttttaac aaatacaaag
     2341 gtaattaatc attacaagaa atacctcatc tgataaattg actaaagtca tataaattag
     2401 taaatcttaa cgttaaacat ctaaaaaaat aaaataaaaa ataaaaaaca agttatattt
     2461 gagtaataat gaattcgatt aagatgcctg atattaaaga tataaatgat atagaaacta
     2521 taattttaga tttttacgaa aaagtaaaag ttgataaatt attacgtcca tttttctttg
     2581 gtgagaaacc aaccaaatgg gaaaaacata ttccattaat gataagtttt tgggagaatg
     2641 ttttgtttta ttctggaaat tatgaaggcg atccattatt gattcataga aaaataaata
     2701 aaattaaagc cactgaaaca aaacactttg agcgttggaa taatttattt gatgaaactg
     2761 taaatgcaca ttttaaaggt gcaaatgctg aaaaaatgaa acaacacgca caggcaattg
     2821 ctgaaatcat gctttcaaag attgaaccca gtaattagtt ttacaaaata gtattccttt
     2881 gtacgccttc cattcttact aataaatcaa gtatttttag tttagaaaag tgtatttttg
     2941 aaatatgatt agaccacgat ttctacaaat aggagatact atcggaatta cttgtccagc
     3001 aagaaaagta gcattagagg aaattcagat agccattcaa atattagaaa gttggggttt
     3061 gaaagtcgtc ttaggtagca caatagggaa agcaaatcat cagtttggag gcacagataa
     3121 agaacgctta gatgatttac aaaatatgtt aaacaatcct gatattcgtg caattattgc
     3181 agcacgagga ggatatggaa cagttcgaat aatagaccaa ttgaatttta atacttttat
     3241 ggaacaacca aaatggctaa ttggtttttc agattttacc tatttacata ccgtattaaa
     3301 tagcaatatt ggcatagaaa ctatacatgg aataatgcca ataacctttg aaaaaagtac
     3361 gcctgaagct ttagaatctt taagaaagtg cttatttggg gaaagtttgg aatatcattt
     3421 ttcttcccat aaattaaatc gaccaggaac gatagaaggc gaaattgtag gtggaaactt
     3481 atctatttta tatagtttat taggtacaaa aacaattgta aacacctcaa attcaatttt
     3541 atttattgaa gatttagatg aatatttata tcatattgac agaatgatga tggctctaaa
     3601 acgtgctaat aaactctcta atttacgtgg gttgatagtt ggtggtatga cagaaatgaa
     3661 agacaatacc attccttatg gcaaaaccgc tgaagaaatt atcttggaac atgtggcgga
     3721 atataactat ccagtttgtt ttgattttcc tgctggacat attgaagata atcgttcaat
     3781 attaatggga aaaaaagcaa agttatttgt acagaaagat aaatgtattt ttaaccaata
     3841 gaaatttact ttttcttatt tgggcatttt tctttttggc aattgccgta tagtgtcaag
     3901 gcatggtttc tcactttttg gtcaaaaagt tctgccgctg tttgcttaat ttggtgtaat
     3961 cttgggtcgc aaaattcaaa tacttcgcca cattccatgc aaattaagtg gtcatgttgt
     4021 cgagagccat aagctttttc atagactgca tgattacttc caaattgatg tttagttact
     4081 aattcacaag ctaccaaaac atctaaagta ttataaacag tagcacgact aacatttata
     4141 cttttattct tgatattgat atataattgt tctgcatcaa aatgaccttc tgtatcataa
     4201 atcatttcca aaatagaaaa tctttcaggt gtttttcgca actgattttt ttctatataa
     4261 ttggttagaa cttctttggc ttcctgttgt attttactta acatcagtat aaaaatacga
     4321 aaagtaattt aaaataaaac taaataaatg cgaaactatt tatatgcacc tataaaagcg
     4381 aaaattattt cccactttta cgttttcttt ctccttcatc taagtaaatt tttctaattc
     4441 tgatagattt tggagttaat tctacatatt catcgccttg aatatattct aaggcttctt
     4501 ctaatgagaa ttttactgga ggtggtaatt tcactttatc atctgttcca gaagcacgaa
     4561 cgttagttag ttttttagtt actgaacaat tcacaaccaa gtcatcttgt ctagagtttt
     4621 ctccaataac cattcctgta taaatttctt ctcctggttc aacaaagaaa cgacctctat
     4681 cttgtaattt atctaaagaa taagcaatag cctcacctgt ttctttagaa atcatagatc
     4741 cattaattct accttgaata tcgcctttgt gtttttcata tttaataaat cggtgagcca
     4801 taactgcctg accagaagtt gctgtaatca tttctgttct taatccaatt aatccacgag
     4861 atggtatttc aaactccatg atagaacggt tgccctttaa tatcatgttt ttcatatccc
     4921 cttttcgttt ggtaatcatc tcaattgccg tacctgctaa tgtttctgga aggtctattg
     4981 taagttcctc aattggctct gatttaacgc catcaatttc tttaaataaa acttgtggct
     5041 gtccaacttg caattcatat ccttctcttc tcatggtctc aatcaataca gataagtgta
     5101 atacgcctcg accatatact aaaaatgaat cagcagtctc tccttgttgc actcttaaag
     5161 ccaagttttt ttctaattct tttgtcaatc tatctttaat atgcctagat gttacaaatt
     5221 ttccttcttt accaaaaaat ggagaatcat taattgtaaa caacatactc atagttggtt
     5281 catcgattgc gataggtggc attgcctctg ggttttcagc atctgctatt gtatcgccaa
     5341 tatcaaaacc ttcaatacct acaattgcac aaatttcccc tgcatgtact tcatcaactt
     5401 ttactttgtt cattccttca aacaacaaca attctttaat tcttgattta gaaattgctc
     5461 catttctctt aattaaagca acctgcatat ttgttttcaa aacgcctcta aatacacgtc
     5521 ctatggcaat acgaccaacg aatgaagaat aatctaaaga ggtaacttgc atttgtgccg
     5581 ctccttcttc aattctaggt gctggaatat gttcaataat tgcatccatt aattctacta
     5641 agtcagtagt tggacgttta tggtctaaac tcatccatcc accttttgca gaaccatata
     5701 aagttggaaa atctaattga tcctcagttg catctaattg gaacattaaa tcaaatactt
     5761 tctcatgtac ttcatcaggt gtacagtttt ctttatctac tttattaata acaacaatag
     5821 gttttaatcc taattgtaat gctttttgta acacaaaacg agtttgtggc ataggtcctt
     5881 caaaagcatc tactaataat aaaacaccat ctgccatttt taatacacgt tctacttctc
     5941 caccaaaatc ggcgtggcct ggcgtgtcta ttaaattaat tttatagtct ttatatttta
     6001 ccgcagcatt ttttgcagta attgtaattc ctcgctctcg ctctaattca ttattgtcta
     6061 atattaaaac tccagtattt tcatgctcag aaaatgtttt acattcccaa aggattttat
     6121 ctactaaggt agttttacca tggtcaacat gtgcgatgat ggcaatgttt cttactttta
     6181 agttttgtat catatatatt taaaactatt ttttttgata ttaattctga ttccaatcat
     6241 taataagcct aaataattta gaaaaataat tagaattagg gtgcaaaaat acggttttat
     6301 tttttgcctt tagaataaga aaagtttaag agaatagaaa ccaatgcatt ctattagatt
     6361 gatttaaaat tgaaaatgaa tactaagaca acaaaacaat tagaataatt caagcaagac
     6421 tatcacttaa tttcaacagt atttatgtca aataaaaaag gcattccgaa gaatgccttt
     6481 gtggctgtgg tcccacctgg gctcgaacca gggaccacct gattatgagt caggtgctct
     6541 aaccgactga gctataggac ctttttgaca ttgaattagc atttctatct aatccgtcaa
     6601 aattgactgc aaatgtagta ttttttttct gaaaaaatac ttacaatagc atttttttat
     6661 ttttaagggt taattgttaa aaacacataa gcaaacaaat tcactaaaag tactacacca
     6721 tagacaaaat tactttttcc acctattaat gatagcatta cggtaaagac agatatgact
     6781 aataaaataa ttggcaatat ttccagtcct aaaacaagag gtaaatcgaa cagatagctt
     6841 acaaaagaaa cagaaggaat tgtcaatcct atacttgcaa gtgctgaacc caaagctaaa
     6901 tttatactgt tttgtaattt attttgctta gctgatgaaa ctgcagcaat tccttctggt
     6961 agtagtatta cggcagcaat aattattcct actaaacttc gaggaagtcc gtaagaagtc
     7021 aatagacttt ctattggtgt agaaagtgat tttgcaagta atacgactat tacaagacat
     7081 attagtaaaa atgtcagact aataataaat gctatgtttg aaatgttgtt atgtttattt
     7141 tcggcattag atgttgactc cgtaataaaa tactctctat gtctaatggt ttgggcagaa
     7201 ataaatgcta tataaataac taaacaagct ataatttcaa atagcaattg tctttctgta
     7261 taataaggac caacaatgct actagtaaat gtaggcaata tcaatgtaaa tgtaataatt
     7321 gacacaagtg caactagagc aatttttacg gaatgaggag aaaaagtctg ggtatgatgt
     7381 tttctacctc caataaaaag agaaagtcca ataattccat ttaaaatcaa cataaccgca
     7441 gcatatacgg tatctcttgc aaatgaagca tattcatttc cacctgtcat cattaaggaa
     7501 ataattatag caacttcaat aatggtaatt gatactgcca agataatagt tccataaggt
     7561 tcacccactc gatgtgcaac tatttcagaa tgatgaaccg cagacattac acaaaacaat
     7621 agtaaaatcc cagcaaatac ttgaaaaaaa acattattat ctaataatcc tgaaaaataa
     7681 agtaaacaag taattattgg taaggaagtc gtccactcgt ataattttac tatttttttc
     7741 atattatatt tatttacatt tccaatttta gatgaaataa ttatctgtac ttagcattgc
     7801 aagaaataca gatgttattg caaaatctgg aatgattgaa aaaattgttc attttttaaa
     7861 tctgttactt gctttttatt gcctgctatc atcacttgat acaaagcatt atttgcaaag
     7921 aaataacgta atctataata agctttacga cttgtaaaaa catattcttt agctggaaat
     7981 ttatacccaa ataaattagt ttcagatttc aatttccctt ttccttctaa ttttaatgca
     8041 tcaaaagctg tcttaaacaa ggaatcagca gttataccta attggtaagt gtagcttctt
     8101 tcactgacta aataagataa acttttatcc cctttttgca aataataatt tttaattaaa
     8161 taataggagc tatctgcttg ttgtacaaca taagtttttg aatttggctc gataggaaat
     8221 ttgcttgtaa aatttacttc tttaaaaact gcccaaggaa tttcagaact cgcttgctta
     8281 tttttttctg caacattatt ttcattacta tttatactag aaaaaggtgt gaaagaatca
     8341 aaaaagtaat ctacaaccgt ttgattttta tttgtttgat tggcaactga agacaatata
     8401 actaaatatc catctatata aaaaatacga ctactaattg cttttccttc taaagtatga
     8461 atcaacacat aatcataatt acgaccattt ttatcttttt ctttataaac attcggatag
     8521 ccaccatact gaactgccat tctatttatt tcttcttgag tagctttttt ttgttcttta
     8581 gtactcactt gtcgcatcga aacagaaagt gtcaaatcaa ttgctgttaa ttcagattta
     8641 aaaaaataca aggtttgtaa tccattagtg gtgtatatat cttttttttc aagattcgtt
     8701 tgttgcggaa attttatcgt tgcaaataca aatttcaatg gtgtatttgc ccattttgtg
     8761 gtatcattaa ttgaactagc attttgtgca aaaagagagc taattgacat caacaaacaa
     8821 aaaagaatac agaaactgct ctttttaagc atattcataa atttatagcc taaagttata
     8881 tcttaaacat taatcattac tggcatcacc aacatcatta aatcttcatt ttcattttta
     8941 tctgttggtt taataattcc agctctgttt ggtgtagata attccattac aatttcagca
     9001 ttatctaagg tagataacat ttcaattaaa aattttgcat taaatccgat ttccatagga
     9061 tcgccttggt attcgcaaac taatttttca gaagcttcat ttgaaaaatc caaatcttgt
     9121 gcagaaacac tcaattcaga acctgataat cttaacacca cttgatgtgt cgttttattg
     9181 gaaaatatag aagttcttct caatgcagca tgtaaatcat ctttattgat agtcaataaa
     9241 tttggattat ctttaggaat tacagcattg taatctgggt atctagcatc aaccaatctg
     9301 caaatcaatt ttgcattgtc aaaaacaaaa aaggcattgg atttattgaa ttgcaaactt
     9361 acggcagtat cattatttgc taaaattcct ttcaacaaat tcaaagcttt tttcggaacg
     9421 ataaatgaac ttggatttga agatgtaata tcacttctat tgaattttac taatttatgt
     9481 gcatctgtag aaactaatgt cattccattt tcatcaattg taaagaacac accagtcata
     9541 gctattctca attcatcgtt acttactgca aaaatggttt tttgaattcc atttgccaaa
     9601 acatttgaag ccacagaaac ttgttctact tcatcagcaa ctggttcttt tggaaaatct
     9661 gcactatttt cacctgacaa tttatattta ccattatctg tagtaatttc aatagcattt
     9721 gtattttcat caacagcaat tgtaattggt tgttcaggaa gtgctttcaa cgaatctaat
     9781 aaaatcttag ctggaatagc aattttgaaa ttctctttag actctacttc catttctaca
     9841 atcattgtcg tatctaagtc tgtggcagtt acagtaagct ttccattatt tatatcaaac
     9901 aaaaaatctt ctaaaatagg caatactgta ttcgaactga ttacgccact aattttctgt
     9961 aaattcttta aaagttgagc tgatgatatg ataaatctca taataattct atattttata
    10021 gacaaaaata catttttttt attacaatac caatgctgat aaaaaatgat ttgactttag
    10081 cctattcttg tgtaattaag ttattgaaat actaatattc attaaaaata ctccagcctt
    10141 ttgatattga taaattgaag catattatct atttcatttg gagcaaacca ctgtatttcc
    10201 ttatcttttt taaagaaagt catttgacgt ttggcatatt gacgtgtgtg taattttatt
    10261 aattcaactg cttcttccat actcgttcta tggtctaaaa aatcaaataa ctctttgtat
    10321 ccaactgttt gtaaagcatt attttttttg tgttcataga cagattttgc ttcatctaaa
    10381 aagccttcct ccatcatttt atctactcga ttgtttatgt ttttatgtaa aatttcttta
    10441 ggcaaatcca atccaatttt gataggcata aaatttcttt ctattgattt tttggtttta
    10501 aagtctgagt atttcttgcc tgttccaata cagatttcca aagcacgaat aacacgttga
    10561 ggattcataa tatccaacgt attataggaa tcaatatcca aaatttttaa tttttcttgc
    10621 aatggcacaa ttccttcatt tctaaaaatt gcattcaact cgcttctaat ttcaggatta
    10681 acttttggca tttcatcaaa cccattcaaa accgcattag tataaaagcc agttcctcca
    10741 cacaaaacaa cgatatcctt tttttgataa agcgtattta acaattccaa aacttcacac
    10801 tcataagttc ctgctgaaac atattcatca atagatttat tggctataaa atagtgcttt
    10861 actgtattta attcttcttc acttgggcga gcaactccaa tatttaattc tctaaaaatt
    10921 tgtcttgaat ctgctgaaat aatgtctgtg tcaaaatatt ttgctatttc gatagcgact
    10981 ttcgttttgc caactgctgt tggtcctgct ataacaatta ggtatttttg tatttttgtt
    11041 ttcaatcaat ttcgacatta aagttcactt tgcaaccttc agaacaattt ctacaaatat
    11101 ctatttcttt tctacctttt gtaatttgtc gtctgaaatt ccaataattt tgattgtgcc
    11161 aaattgtttg gaaactatct ttttgcaaat cgcccaatat atgtttcgca tctttatcaa
    11221 aacagcaagg cactactttt ccgtcccaag taactgcatt actatgccat aatttccaac
    11281 aagcatcatc taatttattt ttgagtgcat actttccatt actcaatttt ttatatctgg
    11341 aatatttttc ttgcaatgga attaaatcat taccgttttc ataattatac acttgggcag
    11401 tttttaaaat aacctcatct acttgcattt cttttgcaat cttttttaaa tcttcaattt
    11461 gatgttcgtt gtgtcttacc accaaaaact gaaagataac ataaggcgaa gtactcttca
    11521 atgctttctt ccatttgatg atattttttg ttccttccaa aactttatcc aaatgaccgc
    11581 ccactcgata ttgctgataa gtctcttgcg ttgttccatc gatagaaatt atcaatctat
    11641 ccaatccaga cactaccgtt tctttagcca cagcatccgt taaatagtgt ccatttgtag
    11701 aagtagaagt atatatctta ttatttttag ccacttttac catttttaga aaatctctat
    11761 tcaaataagg ttctccttga aagtaaaaag ttaagtacaa caaatgttgc ttgatttctt
    11821 gtattgtttt ttcaaaaaat gctggctcta acatccctgt atttcgtgta aaagcacgca
    11881 aaccacttgg acattctggg caacgtaaat tacaagaggt tgttggttct atagaaatag
    11941 aaaacggcaa accccattga attggctttt ttaagtattt tgtcaaataa aaagaagtgt
    12001 aaactaaaag catattgtac agcttcccaa ttgtcaaaca ttttaaaatt gctaagatgt
    12061 gttgttgctt aaacatcttt gctaaggtac gatttcaaaa ggataaaata caaaattctt
    12121 taattaccta aatcgtaaca attgttatcg agagctataa aacgctgatt attttatgta
    12181 atattacaag attgaaaatg aatagtaaac aaaaaatatt aattgcattt ttagattggg
    12241 ggcttggtca cgccacacga tgtatgccta ttgttgatgc attattggaa cgaaattacc
    12301 aagtaataat tgctggaaat ggcaaatcat tactgatgat gcaagagcaa tatccaaatt
    12361 taaaaagcta tgaactgcca gactataaca ttcaatacaa tggagacaaa aatgctgctt
    12421 ggcaaatttt atttcaagct agaaagattt acaatgctat aaaaaaagaa catcaatttt
    12481 taagcactat tctagagata gaaaatatta atattattat ttcagacaat agatatggta
    12541 tttatcatca aaattgtaaa aacattttca taggtcatca aattgcaata caagcacctc
    12601 gaccttttaa atttgtcgaa ccatttttat taaaacaaat cttaaaattg atttcaaaat
    12661 ttaatgaact ttggattcct gatatggaag atgaagtcca taatttatca ggagttttat
    12721 ctcataaaat taaatttaca attcctgtaa aatatatcgg aattctatca aggtttaata
    12781 aagataatca ttcatcaaaa atcgacgata aacgatataa gattgttgct ttaatttctg
    12841 gagtagaacc cagtcgctcc gattttgagc aaaaaataat aaaatcatta gaagaaacaa
    12901 atagaacctg tctattaatt tgtggaagaa ttgaaaaaca aaaatctata aaacaacata
    12961 aaaatattac aatcatcaac tttttgcaag gtgataaatt agaacactat ttacaacaag
    13021 ctgaagtagt tatttgcagg tctggttata gtactgtaat ggacttaatg gctttagaaa
    13081 aaaaagcaat ttttattcca acaccaggac aaacagaaca agaatattta gctgaaaatt
    13141 tagctaaaca aggtattgca atttatcaaa agcaatcgtc catgaattta gtagctgctt
    13201 tatcacaaat tgaatctata aaaggcttta aaaacaatac ctcttttcta ttcagaaaag
    13261 aagtattaga tgatttataa gaataagcta ttatacagaa acaccgaaat ctctcaatgc
    13321 atcattcaaa gatgttttag tatcagtact ttcctttctt tgtccaataa ttaaagcaca
    13381 aggcacttga aaagttcctg ctggaaattc tttaggtatg gttcctggaa taactacgct
    13441 tcttgcaggt actacgcctc tatattcttt tggttcagtt cctgaaacat caataatttt
    13501 tgtactcatc gtcaatacaa catttgcacc caaaacagct tctttctcaa cccgcacacc
    13561 ttctacaaca atacatcttg acccaataaa acaattatct tcaataatta ctggagctgc
    13621 ttgtacaggt tctaaaacac caccaattcc aacacctccg cttaaatgta catttttccc
    13681 aatttgagca caacttccaa cagttgccca agtatccacc atagtaccac tatcaacata
    13741 agcaccaata tttacataag atggcatcat tacaacacct ttttgtagga acgctccgta
    13801 tcttgcaatt gcatgaggca caactcgaac acccaatttc tcgtaatttt tctttaaagg
    13861 aattttatcg taaaactcaa atggaccgac ttctatagtt tccatttttt gagttggaaa
    13921 atacaaaatt actgcttttt taatccaatc atttactttc cagccatttt cagttggctc
    13981 agccactcga aattcaccac tatctaattt tgcaattacc tcatttatgg catctaatgt
    14041 acttttttct tgcaataaag ttctattttc ccacgcttgc tcaataattt gttgtatttt
    14101 atccatttta ttccgaaatt tgttttgcaa aaatagcctt tttaattaag tttacgaaca
    14161 acaaaaaaac aaagatgcat tttagaatat atatcctgct ttttacagta cttttatttt
    14221 ctgcttgtaa aacaagaacg gttgaagaaa aagacctcaa tggaaatatc ctaaaaaaat
    14281 atagtgtttt aaaaaaagat gtgtcaaaaa aacatggagc atacactgct tattatgatt
    14341 caggcgaatt attggaaaat gcaaattaca aagatggaaa attagatgga gaaagaattt
    14401 tgtattttca aaatgggaaa ataatgagta aagaacacta taatatggat aatatggaag
    14461 gtgtttatga aaattattat gagaatggaa atataaaaca aaaaggaatt tataagaaca
    14521 atcagatagc agatacttgg acgaattatt atgaacaacc agctaatcaa ataaaagaga
    14581 gctttacaat aaaagatgga atgattaatg gtttgtataa agaatacgca acaaatggca
    14641 cattaatcat tgaaggcaac aaagttgaag tagaagttgg tttagatttt tttgatggaa
    14701 aagttactat gtttgatagt atcagcaaaa aacctgtgat ggtgtttgaa tttaataaag
    14761 gaaagatgat taaaaaagat tcaattatat aaaaaataca ttttatgaaa aaagttaatt
    14821 tgctgttatt gagcttaatt ttatttcttg caacagcatg caagatgcca gaaccaaagg
    14881 aattgggagt gaaatttgaa gaaaataatc caatttcaat tgatgactta attgctcaat
    14941 tacaaacaac tccattaatt aaagctgttc aattaaaagg agaaatcaat aagtcttgct
    15001 taagtgaagg ttgttggttt acaattaaag ataaaaatgg agaaagccat actattgatg
    15061 taaaagataa gaaatttaga ttgccgaatt caagtgctgg aaaaactgta gttgccttat
    15121 tagatgctaa aattgataca acacaagacg aaccagaaat tattttagaa actattggga
    15181 ttaaatatct tgatgaaaat tcatcaactc aataaagatt tattttttaa atttcctgat
    15241 tgcaataatt tttgaagata tttgtcttta aggcaatcat ctaaatatgc aatatgtttg
    15301 ggtttatgaa tatctgtacc tacaaaatct atcatattct cttttactaa gtgttctgct
    15361 accatttttg caccttttcc gtaaacacct aaaatagaaa acagatttaa ttgaaacaaa
    15421 acaccttgct ctttgatttg ctttagttgg cttaaatttt tatcataaaa ataagcataa
    15481 cgttctggat gagctaaaat aggttggtag tctgtttggg aaatattcat gaatatattc
    15541 tgataatcat tctgtttttg aaggtaggaa aactccacca aaacataatt ttcttttcca
    15601 aaagaaagaa tgtcgccatt aaatagcaaa tccaagaagt gtgtatcaag ataatattcc
    15661 gctgaggctt caatttcaat atctaaattt tgggactgaa tctcttgacg gacaatttgc
    15721 agtttatttt taatgatttc ggaggtatta tcataagttc ctttcataat atgaggtgtt
    15781 gtgataattt ttttataacc caaccctata aaatgtctaa tgatttcaat gctctcttct
    15841 atagatttaa caccatcatc tatgccagga agcaaatgag aatgcatgtc tactttaatg
    15901 ttttccacta aaactgaatc agatatttct gactttgaga atattgattt taaagaatca
    15961 aacattatag attaactaaa taaatccttc caatacttat actgccaaat tttgtatttc
    16021 tcttcttgtt caaaatctcc ataagcacca tagcctccat attttccata attaccataa
    16081 ccatattcag attttcttat gtcttcagca ttcagaacta atctaaaatc tgcaaagcga
    16141 ccttcattat atagtttttc agatattttt aatgctcttt tatctaaatg atctgctctt
    16201 acaatataca aacacatatc aacaaaagga aacaaagata aagtttctga tactaaacca
    16261 actggagcag agtcaataat tatataatca tatctcactt ttaattgatc catcattgat
    16321 cttaatctat cacttttagt aatatctaag aaaactggtg caatcaatcc aggatatata
    16381 acatcaagac cttctacatt agttgagtta acaatatcct caatttctaa attttcttcc
    16441 ttaataaaat gtgttattcc tttattttta acattcttga aatagagatt taatttagga
    16501 gctctaaaat ccattcccag taagacaact ttattgttcg aataagccaa gaatgccgct
    16561 aaatttgaag atataaatgt cttcccttct cctgcaattg tagaagtcaa cataatcgtt
    16621 ttacttttac tatcactaat atgagagagc gaaaaaacca aatttgtaac taaaactcta
    16681 aaagattcca caatacctct tctgtcatct ttttctaaga ttttagtttc tggtttccta
    16741 gttttaacta ttgaagacaa aactggtata ggtgataagc gttctaattc agataagtta
    16801 tgtattttgg tatccaataa tgataagaca taaattatta ctgttggaat aatcaagccc
    16861 aaaacaagaa atatcaaaat aattttacct ttttgtggag atataggtac acctgaacta
    16921 aaagctgggt taacaatctt tgcatcgggt ttaattgata tagtggagat ggcaacctct
    16981 tctcgctttt gcatcaaaaa taagaacaaa gcctctttgg tttgttgttg tcttgatata
    17041 tctctcaaaa ccctttcctt attaggaatt aaacttaatt ctccttctaa aatattatct
    17101 tgctttctta ggttattgac caacatttgg ttatttaccc ttaaattgtt taaggaagcc
    17161 ttaatattct cacgtaatga acttatgctt tttgaaagtg ctatacgttc tggatttaac
    17221 tcactaactg agcttttata ttgcatatct tgtaatacta aatcattata tgctttaatg
    17281 tttacagcaa tattcacatc agctaatcca atatttacag ggagaatttc attcttattg
    17341 gtattaagtt cacctaacat caaattaata atactcaatt gtaattcagc atcagtaaga
    17401 gcctgtttag tactcatact attatctgca aataatctgc tttcatcaac aatactataa
    17461 accttatttg tcgttttata tttctctaaa tcagattcaa ctccagataa ttcagtacta
    17521 ataacaccta atctatcctg aataaacgca taagttttct cagatactat ttttttagaa
    17581 agtatatcat tattaaaata ttcttgtaca acattattta aaatatcttt tgatttattt
    17641 tcattggagg actctataga taagttaata gcattagaaa cctttgatga tttaatggct
    17701 tgttctacta ctaaattttc ttgcaaattc aataatgttg cagtaatcgg atttaaagta
    17761 atcgtatatt cttctccttc aactgcctga ggcaaatctt ttttaagaat taatttaaaa
    17821 gatactaact caggaatttg caaaggtttt ccaaatggaa atttataagt cttttcattg
    17881 taagtcaatt gatagttttc taagtcaatg atttcaattt taaaaactag tgggtctttt
    17941 atatcaccat tcaggtactt cctttcaatt ataaaaggcc tattgtcata gacgccaacc
    18001 gtcttttgag tatattttgt ttttctagtt actttcacat ctaaaagtag attatcaact
    18061 accttcatta atacaggaag cgactttaca actaacattt gatcttctac caccttattc
    18121 cccccaaata aatcttcaat agatgaaaaa tcaaccatta atgattgatt ttccttttcg
    18181 attaagatag aactcgtcgc ttgataaact gggatagaat attttaaata taaatatgct
    18241 ccgacagaga aaataataat aaaaacaaga tacaatggcc aatatcttaa atattggtct
    18301 aaaatcagac gtacatttat atcttcgtct gaaaattttt catttgaaaa caacggttct
    18361 ttctccacac cttttatttt ttccgaacta ataaaaatac tcctgttata gttgacacta
    18421 ataacgaagc aatagacaca aaaatagggg tattcctatt aaacgcagat gattgtattt
    18481 gagctttatt cggggatacg ataattacat cgttttgttt taaataaaac acttcagatt
    18541 caaaaagatt tttactagtt aaatctattt tataatattt ttgtttacca tttgtttctc
    18601 tcaccaacat tatattatct ctccttccat aaatagttaa gtctcctgct gttcctaaag
    18661 catctaaaat acttactcgt tcacttgttg agcgatacaa tccaggcact ctaacttcac
    18721 ccaaaacagt atatttaaaa ttcaaccatt caataacaac aacaggttca tttatataag
    18781 gctttaattg ttctttaaaa tattctattg tttctaagat ggttttacca gcaatttcaa
    18841 tttttcctaa aactggaaat tctatcgtac tgtcttgtct aataatatag ttcattaatt
    18901 tcggctgggt tgttatattg gagtttccaa cagtccctgc cattgatgtt atattgaatg
    18961 gtgctgttcc taaattatct tttgacgaaa taatgatatt taatacatca tcaggctgta
    19021 atttagttgt atatttctga cttatatctc tttcttttcc tgcttcgaaa tcttgaaaat
    19081 acaatatttc tttaggtgaa gcacatgatg taattaatat aataagtaga agatatagga
    19141 gtttattttt tttcaagtaa tagtttttat tcatagttgt ttcattaaac ttatccactc
    19201 taaaagaaga caagcactca tacaatattt aagaacttac aaaaatacaa ttttattttt
    19261 tatccattaa aaataaataa aaattcattt attatttcaa tatattaaca ccgtattaac
    19321 gatatttcta aaaaatttta aaatataaaa caaaaaagga tagcttttag ctatcctttt
    19381 caatacatta tattatataa tgattagtga tttattgttg cagcgtcctt tgatgcctca
    19441 ataacttcgt tattatttgt agtatttgtc acttcttctg ttagctcttg actttctttt
    19501 tctgttactg cttcagcatc agcagagcaa caagctgctt tttcagaatg aagctcactt
    19561 gtttctacca tttctgtatg acaagaagct ccttgagttc catctacatt taacaaaccg
    19621 ccaacagtta tatatggtgc aattactaaa gaaactatag acatcaattt aattaatata
    19681 ttcattgatg gacctgatgt atctttaaat ggatctccta ctgtatctcc agttacggca
    19741 gctttatgtg gttctgattt tttatagtag atttcaccat taatatctac tcccttttca
    19801 aatgattttt tagcattatc ccaagctcct ccagcattgg actggaacat acccatcaat
    19861 acgcctgaaa cggtaactcc cgccaaaact cctcccaaaa cttcaggacc aaataaaaat
    19921 cctacaagaa tcggtgttac taaagcaata gcaccaggta aaatcatttc tcgaatagat
    19981 gcttgtgttg aaatggctac acatttctca tattctggtt tcgctttata ttccataata
    20041 ccaggaattt ctctaaattg acgacgtact tcatttacca tatccattgc agctctacct
    20101 actgcagaaa tcgccaatgc agaaaaaata aatggaatca ttcccccgac aaataaccct
    20161 gccaaaactg gtgccttata aatatcaatt gcttctatac cagaaacacc cacaaatgca
    20221 gcaaataacg ccaaagatgt taatgcagct gatgcaatcg caaatccttt tcctgtagca
    20281 gctgtcgtat tacccacagc atctaaaata tctgttcttc cacgaacttc ttcaggcaac
    20341 tgactcattt cggcaattcc tccggcatta tctgcaattg gtccaaaagc atcaattgct
    20401 aattgcattg cagtggttgc catcatacca gcagcagcaa ttgcaacacc atataaacct
    20461 gcacataaaa aagatccaaa aatacctgct gccaaaacta aaattggcaa tactgtactt
    20521 tccataccta ctgccaaccc accaataata tttgtagcat gacctgtgga agattgtctt
    20581 acaattgata aaactggacg cttacccatt gctgtataat attcagtaat gattgacatt
    20641 aaagcaccta caactaaacc tacaattatt gctccgaaaa cacctgttga agtaaatgta
    20701 ttacctctca ataccatttc agtaggtaat aaatatttta ctaagaagaa agacgcaata
    20761 ccagtcaaaa caatagctcc ccaatttcct aaatttaatg caccttgtac atctccattc
    20821 tcgttactca cttttacaaa gaaagtacct aaccaggaaa atattattcc cacaccagca
    20881 attgccattg gtaataaaat tggagccaat cctccaaagt tgtcattaga aatagtttct
    20941 tgacccaaaa ccatagttgc taagacagtt gccacataag agccaaacaa atccgctccc
    21001 atacctgcaa catcacccac attatcgccc acattatctg caatcgtagc cggatttcta
    21061 acatcatctt ctgggatacc agcttctacc tttccaacta aatctgcacc aacgtcagct
    21121 gctttggtat aaattcctcc acctacacgt gcaaacaatg ctattgattc tgcacctaat
    21181 gaaaatccag ttaatacctc aatggctttt aataagccat ggctatttgc tgcagcacca
    21241 ccatctgtaa acataaaaaa gaatacaata aatagtcctc ctaagccaaa tacggctaag
    21301 cctgctactc ctaatcccat tacagttcct ccagtaaaag acacttttaa tgcttgtgct
    21361 aaacttgttc tagctgcctg tgttgttcta acattagcca gagtagctac tttcataccc
    21421 acatatcctg caaatgcaga aaatatggct cctattaaaa atgcgattgc aattactgga
    21481 cttgagtttt cacctttcca acctaaaaat gccaataaaa tggcagcaat aattccataa
    21541 taagaaagca ccttccattc tgcttttaaa aatgcaatgg ctccatctgc aatatatcca
    21601 gataactctt tcatatttga atcaccagcg tcttgctttt ttacccaagc aaactttatt
    21661 gctgtgataa ctagtccgaa aattcctaaa aatgggacta cgtaaaataa actattcatt
    21721 ttcctctatt ttgatttatt aacaatttta taaaagagag caaaggtaag ggattttttt
    21781 atatctccta catattatta taacattaat agcaaaaaag cctgagtgaa ctcaggcttt
    21841 tataaaattt aggatgattt tactatttaa tttgaactgt agtttcttct tctatccaac
    21901 attttacagt atatggagct ccgacagtca attttttagc tgtaatatct gctttagttg
    21961 gtaaatattg ttgatacata ctcaacattt tatttgcttc agttttaatt tcttcatctc
    22021 caaattctac tgcttttcta aaataatcaa atgctggcca gagtacaatt tgagattgga
    22081 aaccaacgcc tggaccacac aattttccac ttgatgcata taaataacct atcaaaaagt
    22141 aggctctcgc atttgtagaa tctaattcga ttgctttttt agccaactct ctagactctg
    22201 gaaataaatc tttatcggct ttcatattgg ctaacaataa atacaaatct gacttattag
    22261 aagcatcagg ttcgtgtgct attgcatttt catataacaa ataagcatca tctaatttat
    22321 tagctttgat ataaatattg gctaatcgta cagcataact atagctaggt tttaattgat
    22381 ttaacttttg taataactca acattcaatg cactatctgc acagcctctt gttttttcat
    22441 atacattttg aaccgtacta gggtcatttg gattagcatt atattgttta gtatatatag
    22501 ctaataattt agcacaaacc tctggattac ttttatctgc aaaattttga atataagcat
    22561 tttcaattgc tgttttggca tctttataac tgtttacata ctgaccattt ttcgcaatat
    22621 ttttatctaa ttttgccatc agaacatcat attttgcagt aattgaatct ccagaaattt
    22681 gaccattttg atattgataa ttcaataagt tgaaataggt ttgcatgaca ataggatact
    22741 tatctgataa ttccaatgaa cgctctaaag atttttttgc tccttcaata tcatttttat
    22801 attgaatttg agcttgtgct ttctttgttc ctaaaacata atcatcatta ccatgacatt
    22861 ttacccactt atcgtataat tgaattagag tatcttgata agacgcttgc aatgctggat
    22921 tgtcttttgt tttttcaatt ttatctttta agatttcctc tccatcataa aaaatgattg
    22981 atgaaagtcc aggtgctttg tcataaagtg ttctccaaaa tggataagct ttttcaaaat
    23041 ctttcttctt aatgtactga ctataataga aataaacggg agcagttttt aaactatctc
    23101 catcaatatg gcgtgggcaa tcttgagcct ttaccattcc aatagatgcg aaaaataaaa
    23161 aagaaaaaag nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gttaaacatc
    23281 ggaataccta ctccaaagtc tatgccaaac tcattaatat cattgtttct aatgctgata
    23341 ttagttttag aataatagaa tcctgctcta tattttaact tagaaaataa tttattgtat
    23401 ttcttaatat tcggtaaaat ttctcctcca ataccaattc gccaactgtt attcaaatca
    23461 gcggttgtat tttgttcaaa gcctttgaag ttcttccaca tttgatatct aaaatctatc
    23521 cctactttaa atttataatc gcgagagaaa acaattccaa tattataagc aggaattgct
    23581 ttcattcctg aaagatgatt ggtttgtgtt ttaaatgtat caggagcaaa tgttgtccaa
    23641 tctttacctg aataaaattg ctgtaaatag gaagcatttt ctactttatc attaataaaa
    23701 tcagataagg tttcatcaga ggctcttcca actaaaattt cattatagaa tgattttaat
    23761 tgatttgaat attgttgtcc atttaagaaa aatggtgtat ttactgcaaa accaaaaacc
    23821 attcggtaag gactagttgt atctttttta ttttttaggt ttaattgata ttgtacacct
    23881 aaatcaacat ttaaggcttt ggctttataa gttccttttt gataagaagt ataactggtt
    23941 aaatctgcaa caccgtttgt ttgtgttgtt gtcgctgaaa tattatcaat ccttccgaaa
    24001 atataaccca agcctaaccc aacagaaaca tcttttattt tgaatccatt attccaaaat
    24061 acttgattta aacttccgtt tccatttgtt tccaataaat aagacgaact actatctaat
    24121 tgattatatt ttgttacttc ataatctttt tttgaaaatg gcattaaccc tgctgacatt
    24181 gaccaaaatt tctttactgg aacagcgatg gaaagataat ccaaattaaa actgttttgt
    24241 tttgctttta aattggtcgt ttttaagttt tggaaagttc cagaaattcc aatatcaaaa
    24301 gaaactaaac taactgcaga aagtgaagct ggattaatat aactttgacc ttctaaagag
    24361 cgataagcag ttcccaagcc tcccatcata ttggttgctg tattattatg attagccata
    24421 attcctaatc catatctact ataaactgag ctttcttgtg ctgtaataat attgtttaca
    24481 attataagtt gtaacaataa gaaaattatt tttttatgca ttgtattcta aaatgtgatt
    24541 tagtccaact aatgttaaat tggggtgtac aaatatctta tttttaagta aagaaaccaa
    24601 aaaaactgca tctccacctg taattatagt atttaatttt gaaaatttaa cgtgataatg
    24661 gtttatcatg gcatcaattt ctaatgctgt tgccaaagat gttccaactg taaggttgtc
    24721 ttctgttgat tttccgacaa aatttttagg cttattcaaa tcaattagtg ggagtttttt
    24781 agtaaatgtg tgcattgctt tcaaacgcat tttcaatcct ggatggatac tccctccttc
    24841 aaattcattt tttttattaa taaaattata agtaatgcaa gtgccacaac caataaccaa
    24901 attattctga cttgggtact ttgcctgaat tcctgcaatc aatgctattc tatcataacc
    24961 taaagtattt ggagtagtat aattattctt aaatggcaat tttgtttgtt cgcctaaaac
    25021 aatatttata gtttgtgatt ttaagacctc taataactta tccgagatta gttttgtact
    25081 tgaaacaata gaatattgta ttttgtattg tttaattatt ttgcgaaccg ttactatact
    25141 caatctatcg acactccaat cttccacaag ttgcatttca gagaaaacgc ctactttggt
    25201 tctagtattg ccaatatcaa ttgttaaatt caaaattaaa tttcagtatt aaaccgccaa
    25261 aattacaaaa aaaatatttg ccaaaaaagt attgtgtcgt ttagaataga ttgactatta
    25321 atttgttttt gtgttttaat aattcctttt aaggcaattt aaatttcatt tagctctatt
    25381 tatatcacta aaaatgttgc atttttctta aaaagatata tttttgaaat aaaattttta
    25441 aaaatgagta tttcacattc actactatca gaactaaaac acgaagctga aagtaccaaa
    25501 aaccttttgg ctgttgttcc attggagaaa ggcgattgga aacctcatga aaaatctatg
    25561 agtttaagta ctttaacaat tcatgtggca gaattatttg cttggatagc tgatgtagtt
    25621 caagagacag agttagattt tgcaaattat caacatcctc cgaaaataaa tacaactgaa
    25681 gaattgctaa attattttaa taatgccgtt caaaaaagtg aaaatgcatt acaaaatatt
    25741 gacgatgctc atttctttga aaattggact ttaagaaaag gcgatcaagt gttttttgca
    25801 ttaccaagag ttgctgcaat tcgttctttt gttttaaatc acatttacca tcatcgtggg
    25861 caactcacag tatatcttcg tttgctagat gtaaaacttc ctggaattta tggaccatct
    25921 gctgattcag taatgtaaga aatggctata aatttttatt ttgatgaagt acaaaagcca
    25981 aaagacttta aaactagacg ttttaaagtt tggttaagtt ctatagaaaa gtattatcaa
    26041 acaaagattg atgatttgtc ttatattttt gtgaacgatg aacaattatt ggcaattaat
    26101 caaaagtatt tagcacacga tacttataca gatatcctca catttgattt gtctgaaatt
    26161 gaaaagcata ttaacggcga aatctatatt agtttagata gaattttaga taatgctcaa
    26221 aaattcaatg ttccaatttt agatgaatta ttgcgagtta ttgctcatgg attattgcat
    26281 ttaattggat ttgatgacaa aaccaaaaca aagaaatcta aaatgactga acaagaaaat
    26341 atttgcatcc agttatatca ttctttactt attgaaaaaa ataatcctta actatacttc
    26401 aataatattt tagcaatctt ttcagcactc ttttcccaac taaataaatt tttacgttca
    26461 agaccagctt gctttaattg cttgtacaaa actttatttt ctactaaatt attcattgtt
    26521 tccgctatct gatgaatatt gttgggttca caataaactg ctgcatttcc tgcaatttca
    26581 ggaatagcac ttgtatttga tgcgataatt ggaatgccca attccatcgc ttcaaccaaa
    26641 ggaataccaa atccctcaaa ttcgctcaaa taagtgagtg cataagcatt ttttaagtat
    26701 tcaattaagg aactgcgatt gattttttct atccaaataa tatccttttg cacacttgat
    26761 tttcttaaat tagttttaaa ttcttcatta tgccaagcat ctctaccgat taaaaccatt
    26821 ttgaactctg atgaggtttt ttgtttgaat ttttcaaatg cttctattaa attcaataca
    26881 tttttacgtg gatgtatagc tccaataaaa ataaaataag gaacatttat tttaaactta
    26941 tattcttgac tgtcattttg tgcttcaaaa aatgcattat aaaccacttc aattttattt
    27001 gaatctagct tatattgttg aataatatct tgtttggaaa actctgaaac tgtaataatt
    27061 ttagcagctt tttcaatata ttttggcgta taataacgca taaatttagc atgtaaaaaa
    27121 ggaatatatt ctggaaaatg ttcaaatgca atatcgtgga ttgttaatat ttgaggaata
    27181 tttgtattca aagaacaata accatctgtt gagagaaaaa tatcaatttt atctttttcc
    27241 atttgtttgg gtaatacata atcaaaccac catttccata aaatagggtg tctagttgga
    27301 ggaaaaactt ttttcccaat tatattttct gaatagataa aacttaggtc aaaatctcta
    27361 tcaaaataaa aatagaaagt atgctcaggc atcaatttta ctaggcgttt gcatatttca
    27421 tcagtaaaaa caccaatgcc ttccatttta tcctttaata aaaaacgagc attgactcct
    27481 attcgcatat accaaaagta tagaatattt ttatatttgc attaatgtat ttacacaata
    27541 ccacttttaa agtcgctttg gcacacaata atgattttat ttattttata aaagatgaaa
    27601 ttattccaca atatcaggaa gaattagaac tcattgacat tgttctttta gaattgctaa
    27661 atgttgatat ggaagacgga aaaacttatt gcctacatat tgtctttaaa gatttacaag
    27721 gctataatga gtataaaata tacaaagaag gtaaactcct aaaagccatt tttgacaaat
    27781 acaatgaaaa tgttttgttt tttagtagta ttttaaaaaa agtagattaa ttgtatttca
    27841 aagcaactaa ttctactaat tcagcaagtc tattggcata tccatattca ttatcgtacc
    27901 agcctacaat ttttgtcaat ccaccatttt gttgtgttaa tcctgcatcg aaaatacaag
    27961 aataaggatt gtgaataata tcgctagaaa caattggatc ttcagtatat tgcaaaatgc
    28021 ctttaaattt ttctttagac gctttatgaa atacttcatt aatttcttga attgatgctt
    28081 gcttttctaa ctgtacatac aattcggttg tagagccagc aataacagga acacgaactg
    28141 ccccgccaat aattttccct ttaatttctg gcataaccca ttctaatgct ttactagcat
    28201 ttgttgtggt cggcacaata ttttgtgcgg cagctcttgc acgtctaaaa tctgttggat
    28261 gtatagaatc gtgtaatttt tggtcttgtg tataggcatg aattgtattc atcataccat
    28321 attgaatgcc ataattatct tgtaaaattt taaccattgg ggctaaacaa tttgtggtac
    28381 aagaagcatt ggaaagaatt aaatcatctt tagagattat attgtcattg atacccaata
    28441 caattgtttt tacttcatta tcagcaggag ctgaaataat aacttttttt gctccagcag
    28501 caatatgttc agctaattta tctttcgttc gatatcttcc gctacactca attacgatat
    28561 caatatctaa gtctttccaa ggcaattggt tgatattacc aaaagaagtt gataggattt
    28621 ctcttccttc gatataaaat ttatcatcaa tctcttttat ttctttatcc cactttcctt
    28681 gagcagtatc atattttaat aaataaacta aagcagcatc aggtgctata tcattaatag
    28741 caacaatatc cactaaattt ttatcaaaaa ggatttgaaa aaccaatctt ccaattcttc
    28801 ccaatccgtt aatagcaatt ctaggtcgtc tcattaggta tatttttcat aaaattacga
    28861 tagaaaaata caaatgcaat aataatttta tgtaaactat tgtgagataa aaaaaatggt
    28921 tgtatatttg cactcgcttt agagaacggc ccgttcgtct aggggtcagg acaggagatt
    28981 ttcattcttc aaacaggggt tcgattcccc tacgggctac caaaaaaaac cgcaattttt
    29041 aaattgcggt tttttttatt ctatcaatat catttttcta ctatatttct tgattattct
    29101 acgctgttgt atcaatcctt agcattcata ttttgttcaa tttcgtaata aatggctaaa
    29161 aatttaattc catctttact ataaatcaga caagatgaat aataacagaa ataaataaag
    29221 tggctattac aatctttcta atcgaatctt cgattttttt gcaatattga taggaacttt
    29281 ctctgttcgc acattggttc tctctaagcc aaaatcgact tcaactggca cacttaatga
    29341 ttttatcatt gcataagcat ccattgccaa tttattaaat gcattcaatt cattgtcagc
    29401 agacacactt gaagtaatca gtacaaattt aaaatctgct ggataattgt gttttcttaa
    29461 agatggataa tgactaagcg tatcgatttc tccattttta cccatttctt ccacgatttt
    29521 cataaacatc gcatgtactt tatgctctac tttaaagcca aattgtaatt taacaaagaa
    29581 gcatttctct ggaataattg tatctacaga ataaacagct ccataaggtt cgtttgtaat
    29641 ttctacatgc acaaaccaat aagcatccgc tcttttaggt ttctttctaa acaaagaata
    29701 aataatattt gaatcaattt ttcttttgtc attagaattt accaaataaa ctaaatttgt
    29761 tgcttctctt ggaattgtaa tatcgttttg caaatcaatt aattgaggta ataaatcatc
    29821 catttcaaca aattctaaat gacggtttct tatttttctt ccttcattaa ataaatacat
    29881 tgacccaaaa attacaattg ctatgacaac tgtaaaccaa ccaccatgtg cgaactttaa
    29941 tatactagat aagaaaaatg aaccttctat cgaaataaat aaaataagaa gaccataaac
    30001 tatccacttc gactttcttt ttataattaa ataataagct aaaagtgcgg ttgtcattag
    30061 catattgatt gtgatagcca gaccataagc tgcttccatt ttactagatt ctctaaatac
    30121 taaaacgact gcggtacagc caaaaaacaa taaccaatta attgaaggaa tataaatttg
    30181 accttgctct tcagtaggat aatttactat catattcggc caaagtctta atttcatcgc
    30241 ctcattcact aaggtaaaag caccagaaat taaagcttgt gatgcaataa ttgcagcagc
    30301 tgtggcaatg gcgataccat aaggcaaaaa ctcacgaggc attaatgcat aaaaaggtgc
    30361 cgtttctaac caattatatt cgccattatt atgactcaaa ataaaagatg cttgaccgaa
    30421 ataattaagt actaagcaga ttttaacaaa agtccaactt actctgatat ttgattttcc
    30481 acaatgtcct aaatctgaat ataaagcttc agcaccagtt gtacataata atactgcacc
    30541 taaaaaccaa aatccgccat cataattgaa aattagatta aatgcatata ttggattaat
    30601 tgcttttaaa acaaatggca ttttaatcac attgataaat ccaaaaacgc ctatcattac
    30661 aaaccaaata aacatgatgg ggccaaataa tttacctaca gtatctgttc cagaacgttg
    30721 aatcataaac ataaaagcaa taataactaa tacgatggga actgtatgta attctggata
    30781 tagtatcgtt aaaccttcaa cggctgatga aatagaaatc ggtggtgtta caaagccatc
    30841 tgccataagt gcagcacagc caattagtgc aggtacaatc gtccattttg ctttatatct
    30901 acgaaccaat gcatacaatg caaaaattcc tccctctcct ttattatccg catttaatgc
    30961 tagatatata tatttgaaag aagtaataat cgtcaaagtc caaaatatac aagaaacgcc
    31021 tcctaatatc aattcttcag taacagcatg attgccttct atggacattc ccataattgc
    31081 tttgagtgta tagagcgtag aagttccaat atcgccatat acaataccaa gtgtaattaa
    31141 gcttcctgct atactaagtt tgtctaactt attgttttct ggcataaaat atgtttgcgt
    31201 tgaattgcaa agctaatttt tttgataaat aatgcaatat tatttttgtc ttttttttgc
    31261 ttcaacacat aatgtcgcat gaggaactgc tcttagtcta tctttaggaa taagctgacc
    31321 agtttctcta caaatgccat aagttttatt ttgaatcctt gataaagcat tatctaaatg
    31381 agcaattagc ttttgttgtc gtgctatcgt ttgatttaga gattcttttt cagatgtaaa
    31441 agcaccatct tccattccag tagattttgt atcattttca gcagcatttt taatttgctc
    31501 ttgatagaaa cttatttctt cttgtgcttt tgccaattta ccttcaatta aaatcttaaa
    31561 ttcttgcaaa tcttcatcat tatatcttac caatctacct tgaggtttta caaggtcttt
    31621 ttctttatgt acgtaagttt cggtagtcat tgttggtgta ggctgtgtcg aaacgtgtgc
    31681 ttttgttcta gatttctcta aggcacttaa ctttgttttc actacaactt tcttaactgt
    31741 tgctgttgca ttaactgaac ttgctttagt ttccgttttt ttaggagcta ttttagtaga
    31801 tggtttagtt gcaacaactt tcttcgctgg agttttaact gtgcttgctt ttgtcgctgt
    31861 tactttagaa gccacacttg cttttttaac tggaactttt gttgcggatt tctttgtagt
    31921 tactattgaa gtttcaactt tagccgtaac ttttttagtt ggaactgctg cttttttagc
    31981 tggtgttaat gcctttttag tcggcgtttt taccgcaacc tttttagctg gaactactgc
    32041 cttttttact agtgctttcg ctactacttt ttttgctggt gttaatgcct ttttagtcgg
    32101 cgtttttacc acaacctttt tagctggaac tactgccttt tttactagtg ctttcgctac
    32161 tacttttttt gctggtgtta atgccttttt agtcggcgtt tttaccacaa cctttttagc
    32221 tggaactact gccttttttg caggtgcttt tgctactgct ttttttgctg gtgttaatgc
    32281 ctttttagtc ggcgttttta ccgcaacctt tttagcagga actgctgcct tttttactag
    32341 tgcatttgcc actgcttttt tagctggtaa aacaactttt tttgtagtac ttgtttttgt
    32401 ttgggctgtt ttctttgcca tatttatatt ttttttatgg tcattaataa agtttcatca
    32461 ttaatatcaa aagattttgc atcagctaag ttatcgctaa aatctatcga ttcacacaat
    32521 gtttcattac aaatataatc tttaaattta tttataacat tattgagtac caaatggttt
    32581 tgcacattta tttgaatttt atcagcaacg ttgaaatttt gctctttcct atcactttgt
    32641 attttattga caatttctct agcaaaacct tcatttttta aattttctgt aatggtgata
    32701 tctaaagcaa ctgtaatacc tctctcattt gcaaccaacc aaccttgaat atcatcagaa
    32761 attatctcta cttcttctaa taaaatttcg taagtagcac cttcaactgt tatatctatc
    32821 tttttatttt gctctaaagt atttatttca ttgtatccaa attgagtaat ttcatctgca
    32881 actaatttca tttttccacc caatttttta cctaatagtt taaaatttgg ctttactttt
    32941 ttagaaataa tgcctgaagt atctgaaata tattctatct cttttacatt gacttctgat
    33001 aaaattaagt ctttgacttt atcaatttga ttaataaatt ctatatcaat atttggaatt
    33061 aagatttttt gtaatggttg tcttacttta atttttgttt ttttacgcaa tgacaatacc
    33121 aatgaagaaa tatttttagc ataatccatt ctttgttcta atgtcgtatc aattttagaa
    33181 gtatctactt ttggcaaata aaccaaatgt accgattcat atatttttga caattcagaa
    33241 ccactttcgt ttccaactag attgttgtat aaccaatctg aaaagaatgg agcaattggc
    33301 gacattagtt gtgcggttac caacatacat tcaaacaatg tttggaacgc agcattttta
    33361 tcatttgagg cttcggtatc ccaaaatctt cttctacata aacgaacata ccaattagat
    33421 aaatcatcga caacaaaggc ttcgatagca cgtgctgctt ttgttggctc ataatcttct
    33481 aacgctttag cgacttcatc tattaaagaa tttaatctag atagaatcca cttatcaatc
    33541 tccgctcttt cttgatatgg aactaattct tgttctttat atgcaaatgc atcaacatta
    33601 gcataaattg caaagaaatt ataggtatta taaagtgtgc taaataattt tctttgagtt
    33661 tcaagaacgc catcaatatc aaattttaaa ttatcccaag gttgtccatt acttaccata
    33721 taccatcgca ccgcatcggc accatatttt gctattgtga taaatggatc tacggcattt
    33781 cctaaacgct tggacatttt attgccattc ttatccaaaa ctaatccatt tgaaacaacg
    33841 tttttatacg ctacactatc aaacaacatg gttgcaatgg catgtaatgt ataaaaccaa
    33901 ccacgtgttt gatcgacacc ttctgcaata aaatcggcag gaaaattaac gccgcttaaa
    33961 tgctctccac taaatggata atggatttga gcataaggca tagcaccaga atcaaaccaa
    34021 acatcaatta aatcagcttc tcgatacatt ttactgccta atgctgaaac tagaataatt
    34081 tcatctacat aaggtttgtg taaatcaatg gctttctcta aaaatgaagc tggcaaaaat
    34141 cctttatcaa tagattgctt tatctctgtt tgtaattctt cgatagaaga gatacatttt
    34201 tcttccgttc catcttccgt tctccaaata tttaaaggtg ttccccaaaa tcttgttctt
    34261 gataaattcc aatcaactaa attttctaac cattgaccaa aacggcctgt gccagtactt
    34321 tctggcttcc aattgatggt tttatttaat tcaatcaatc tatctttggc agcagtaact
    34381 tttataaacc aagaatctaa tggataataa ataattggct tatccgttct ccaacaatgc
    34441 ggataactgt gctcatgttt ttcgacctta aatgctttat tttccttttt gagttttacg
    34501 gcaatatcga tatcaaccga aatataattt gggtcatctt tataatcttt tacatatcta
    34561 cctgaaaatt ctccagtacc atcaacaaat tttccttctt tgtccaccaa agtcaatcct
    34621 ccaataccat tttcatttgc cactttcata tcatcagcac caaaacttgg agctatatgt
    34681 acaatccctg taccgtcttc tgttgttaca aagtcgccta atataatttt gaaagccact
    34741 ccatttattt tttctttgct attggataca aaaggcaata attgttcata attaattcct
    34801 gctaaatcgg agcctttgac ttcatttaca attgaataag gcagattttt gccatcattt
    34861 tgatagtttg aaaaattagc attttcattt tctgggctaa aatattttcc taataagttt
    34921 tttgccaaaa taacctgcat tggctgatgc gagtatggat ttattgtatt gaccaaaaca
    34981 taatcaatat ttttaccaac tgccaatgct gtattggaag gtaatgtcca aggcgtggtt
    35041 gtccaagcta aacaataaat agcttgattg ctggaaaaca agttttgcaa ttgctcatca
    35101 tctgctttta atttaaaaca agcaactgcc gttgtatctt ttacatcacg ataacaaccc
    35161 ggttgattga gttcgtgtga gcttaaacct gttccagctg ctggtgagta tggttggatt
    35221 tttagtcctt tgtataaata acctttttta tataattctg aaattagcca ccaaacactt
    35281 tctatatatt cattttcata agtgatgtat ggattatcta aatctaccca atagcctatt
    35341 ttgtctgtca attcatccca tttgtcttta aatttcatga cttccttgcg gcactcttta
    35401 ttgtaatcct ctacagagat ttttgttcca atatcttttt tggtaattcc taatgatttt
    35461 tctacttgta actcaatagg taagccgtgt gtgtcccaac ctgcttttct atttacttta
    35521 tatccttgaa tcgttttaaa tcgacaaaaa atatctttta tggctcttgc catcacatgg
    35581 tgaatcccag gcattccatt tgcactcggc ggaccttcat aaaaaacgaa gtttggactg
    35641 tcatttcttt gagtgatgct ttttttaaaa atatcattgg atttccaaaa tgcattgatg
    35701 gattgttcaa tttctgtagg attgaattgt ttatattctt tataaaattt tttgtcagta
    35761 ttagacataa tttgcttagt attagattaa aaatcggtgc aaaaatacga aaaatatagg
    35821 aaaaataata aaaaacctat ggatactatg gctgaaattc agtgatttcg ggaaatattt
    35881 aagttttttg catttctaaa cacaccataa ttattagtga atattagcaa actgcatttc
    35941 atataagttt ttatagtacc catttgatgt gttcatcaga gtttgatgtg ttccttcttc
    36001 aatgatattg cctttttgta aaacaataat tttatctgca ttttgtatcg tcgataaacg
    36061 atgtgctact actatagctg ttctattttc aattaattta gcaattgctt gttgaatgag
    36121 taattcagat tcagtatcta cagatgatgt tgcttcatct aaaactaaaa ttgctgggtc
    36181 ataaaccaat gctctaataa aagataacaa ttgtctttgt cctacggata aagtagcacc
    36241 tctttcttgt acattataat cataaccatt aggcaaatcc ataataaaat cgtgtgcacc
    36301 aacgagcttt gccgcttcaa tgacttttgc cttatctatc gttggatttt taagtgtaat
    36361 attatccaat atacttcctg aaaacaagaa aacatcttgc aaaacatacg caaatgcact
    36421 tctcaaattc tctaaagaat aatcgtaaat attaatatca tcaatcagaa tttcaccttt
    36481 attgatttca taaaaacgat tgaatagatt ggtaatactt gattttcctg agccagtggc
    36541 acctaccaaa gctaaagttt tcccagcttc aattttgaaa gaaatattat ttaaaacata
    36601 ttcatcttct ttataagcaa atgaaacatt tttaaactct attgagccat caaaactttc
    36661 tttttcaata cgtccatggt cttcattttt ttcttgattg tccaacaatt tgaataccct
    36721 ttcggcagca ataacgccca tttgtaatgt attgaactta tctgctagca tacgcaaagg
    36781 tctaaatgcc atattcaaat acataataaa tgcggcaaaa atcccaacat ttgccgtttc
    36841 ttctaaaaca attttacttc caaaccaaag cattaacgcc atagatactg ctaaaacaat
    36901 ctcaacaaat gggaagaaaa tcgaaaaata caaaaccgat ttatcgtttg ctttataatg
    36961 ttcgtaattt atcttttcaa attttttaaa ttctctatct tgagcattaa aaatttgaat
    37021 aatt
//
LOCUS       DAOQCW010000008        35506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_416_length_35506_cov_5.707963, whole genome shotgun sequence.
ACCESSION   DAOQCW010000008 DAOQCW010000000
VERSION     DAOQCW010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 35506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35506
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_416_length_35506_cov_5.707963"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1413)
                     /gene="rpoN"
                     /locus_tag="PLS16_01540"
     CDS             complement(<1..1413)
                     /gene="rpoN"
                     /locus_tag="PLS16_01540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNJ59788.1"
                     /translation="MLGQRLSQKMLQKLSPQQIQLMKLLQIPTSLLEQRIKEEMEINP
                     ALEENPNDFDNQKEDLAEPQEIKEQEDDYENQSNEDDEFGSDLDINEYTKDDYDYNAD
                     DDYTGRQEEKKQMPLTVGKSFHDYLLEQLALQNELSDHEYLIADHLIGSLDDDGYLRR
                     ELEAIVDDLAFQRNISTDEKEVEYILSLIQRFDPAGIGARSLQECLILQLERKKQTKT
                     VELATEVLNKYFDAFTKKHYDKLAKALNISEDELKAVLDEIIQLNPKPGGTNSNSGAS
                     ISHQTIIPDFTVEEIGGKLEIYLNGKNAPDLRISDSFKEMLVSYGKGNVKDKKNKEAV
                     TFIKQKIDAAKWFIDAIKQRQHTLFATMQAIVQFQEKYFFSGDEMDLRPMILKDIAEI
                     TGLDISTVSRVANSKYVQTEFGIFLLKYFFSESLQTDSGEEVSTREVKKILSDIIENE
                     EKSKPKSDEDLTELLKEKGYN"
     gene            complement(1582..2577)
                     /locus_tag="PLS16_01545"
     CDS             complement(1582..2577)
                     /locus_tag="PLS16_01545"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNJ59789.1"
                     /translation="MKVAVVGSTGLVGSEMLQVLEERNFPVTELVPVASEKSAGKKIQ
                     FKGNEYTVVTPQQAIALKPDVAFFSAGGDTSKEWAPKFAEAGITVVDNSSAWRMDPTK
                     KLVVPEINGATLTKEDKIIANPNCSTIQMVAVLNPLHLKYKINRVVVSTYQSVTGTGK
                     KAVDQLMNERATGSSDEMAYKYKIDLNVIPQIDVFLENGYTKEEMKMIQETCKIMQDD
                     SIKVTATTVRIPVMGGHSESVNIEFENEFDVKEVIDILSKTEGIIVENDDANQIYPMP
                     MNAHKKDEVFVGRIRRDESQPKTLNLWIVADNLRKGAATNTIQIGEFLLKNGLIG"
     gene            2765..4552
                     /locus_tag="PLS16_01550"
     CDS             2765..4552
                     /locus_tag="PLS16_01550"
                     /inference="COORDINATES: protein motif:HMM:NF040455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNJ59790.1"
                     /translation="MNRFILHLNILILAIVVLVQCKTAKTEDNVNAIINSEEISDKDF
                     NEQISVLIKSEIETLANNKVDTANFTSLQTILHRVYKSTDFQPIWVNKHILNENGKSL
                     FYFLANAELHALIKEKYNYEIIKQLKDSLEHNLPSIQFLLIKKLDIELSKAFLQMALH
                     IDKGIYNEQKKVINKNFFEKEDTYTQLLASIIAGKNPEQALVSLEPQHPMYKRLKAAV
                     YQFVSKSNISPQKISIRNYKTDSIGAINDAKLALTYHHYLHDTLMNDNVAYLSALKAF
                     QKDQGYSQDGVVGNMTARALERDNSQKFRLLLINVDRWRSKKNDIKIEPKYVWVNLPS
                     YKLSIIRNDSLLLQKNVVIGKAGGKTESPEIISAINQIVVWPTWSVPQSIIKNEMKSF
                     KGYIVSKKNGYTSVVQPPGLNNSLGAVKILFPNKHSVYIHDTPSKYLFKSDYRSHSHG
                     CIRCQDPFEVAGMLLNMDNPAIDYDSLIRLKEKKIATKNISLKNPIPVFLQYFTAEAD
                     WNGNLKFYADTYKNDSVIIATIFGKIDNTTKPITIQKIKIDSILNTTNRPLKKDSTIL
                     NKSDSLPKKEDEIILLKNDSFEKSSTEPF"
     gene            4695..6059
                     /locus_tag="PLS16_01555"
     CDS             4695..6059
                     /locus_tag="PLS16_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNJ59791.1"
                     /translation="MENRTKIKSVIILLLLTILNSSILLAQWGTLGNQIKNGLSNKAQ
                     QKIKQEINDGVDKAYDKSKQKTKESVKNSKEKDDESNSNSSSDVSNENNKNSSSNSTA
                     NSSLKAYGKFDFIPGEKVIAQEDFTQDAIGDFPSKWNTNGSGELVTIDGQQGKWLKFA
                     PQSIIYPEFVTNLPENFTVEFMLASTQPFSFYSSAFNFFFAPLVSPSKNYVQWKQYSG
                     GRKDGVLVAIHPEAAGGNAMGQKWITTYDETGAEIVKNQSDFPDFNHENKNVVKVSIW
                     RQKQRLRVYINETKIWDIPKAFSATAKYNFMGFRTDSYHNDNDAYFLSNLRVAVGAPD
                     TRHKFLELGKYSTTGIKFDVNSDKIKGESYGTIKDFADVLKENPDVKVKIVGHTDSDG
                     DDASNLALSKKRAEAVKAILNKEFGIELSRMETEGKGELQPTDTNATAEGKANNRRVE
                     FIKL"
     gene            complement(6093..6731)
                     /locus_tag="PLS16_01560"
     CDS             complement(6093..6731)
                     /locus_tag="PLS16_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HNJ59792.1"
                     /translation="MKTLDKSAKEHLTPEMALQILKNGNERFVNNLEAHRNLLEQVNA
                     TRDGQSPFAIILSCIDSRTSAELIFDQGLGDIFSVRIAGNVINTDVIGSMEYACKLAN
                     SKLIVVLGHTKCGAIKGACDFVELGSLTELLTKIQPAVYAEKTTPTELRNSKNLQFVN
                     NVSAINVRKSVQAILDRSFILEQMIASGEIGIIGAMYDIETGKVDFYEEFHH"
     gene            complement(6876..7577)
                     /locus_tag="PLS16_01565"
     CDS             complement(6876..7577)
                     /locus_tag="PLS16_01565"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNJ59793.1"
                     /translation="MKKIILVLGILVSISTFAKEPITDKKIMIGFNIGTSANSAFTEG
                     EKSYEAKNRTKVGGFAGFTFEGRLADRLSLETGVSYVNKGAANRLKKNLLINSGSSTY
                     NFHSIDIPLIVKFYIGKKKIFNVNGGGFFSYAFKMQQVTNVDFVNPGTPDVKNKKEDI
                     PTNPANKEKLFNPIDAGVNLGLEFISQKGFGAGFKLNQGFINVMNKKFFTAPLTPPDK
                     KTLNTGAQLYFIAKF"
     gene            7709..8791
                     /locus_tag="PLS16_01570"
     CDS             7709..8791
                     /locus_tag="PLS16_01570"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HNJ59794.1"
                     /translation="MQQDYFAIGLMSGSSLDGLDLCYVHFSRKDNLWFSYTILATDCI
                     SYNEAFREILKQAPNLSAINFAKLHIDLGRFFGNTTLDFIQRHQIEKIDFIASHGQTI
                     FHQPQQHFSTQIGCGATIAQVTQVKTISDLRMSDVAAGGQGAPIVPIAEKYLFPNYDI
                     FLNIGGICNIAFHHKTANHILAYDVCAGNTLLNYLAQQKSKTFDEGGQIAQQGNVCAE
                     LLHQLNSIPFCSEKSPKSLGSEHIYQDWIALTDISNISIEDKMATCVEHIAQQIGNQI
                     NQYSTSKSQQLLVSGGGAFNTFLIQRIQNYTSIKVVVPDKATIEQKEALAIAFFGLLR
                     ILQIPNCISSVTGANKNVVGGAVYLP"
     gene            complement(8793..9740)
                     /locus_tag="PLS16_01575"
     CDS             complement(8793..9740)
                     /locus_tag="PLS16_01575"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNJ59795.1"
                     /translation="MFLYFKIENGKLLQIDDASNANWIHITPPFVAKDLEQFALENNI
                     PNSHFTDCMDLYEQSRYEIGKELKQIIINTPIYNNAVDVDDEADYLTIPVGIIQTKEK
                     LFTFSAINNPMMDWCTKNMLEKQLEFTLDTVVLYIFKRTVFYFIFYLREINVQISKIE
                     KNLKHSSSNDGLNKLLKIQKSLIYFLNDLRADELILEKHKEALSIQNDSIENNKIIRD
                     VMIEYSQAIDMSNVYSGILGNMMSAFGSIISNNLAYVVNRLTGVTIVLMIPTLIAGIY
                     GMNIPLPFQEHPFAFSIVTIISALITGVSLYYFKHKKWL"
     gene            complement(9742..10518)
                     /gene="dapF"
                     /locus_tag="PLS16_01580"
     CDS             complement(9742..10518)
                     /gene="dapF"
                     /locus_tag="PLS16_01580"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNJ59796.1"
                     /translation="MNLEFYKYQGTGNDFVLVDNRTLFFPKENHNLIAKLCDRKFGIG
                     ADGLMLLENAEGYDFRMVYYNSDGKEGSMCGNGGRAISKFAIDLGIVTKKEAEFIAVD
                     GKHEFLYKNNLIRLKMIDVEKINAYQNDFVLNTGSPHYIKIVPNVEMDNFVEDAKSIR
                     YNDDFKKDGINVNYISIDDEVKIRTYERGVEDETLSCGTGNVAAALCVAQIKSIDAGS
                     INLITKGGNLNVYFTKQDNQYTDIWLEGPAEFVFKGEIKI"
     gene            10660..12135
                     /locus_tag="PLS16_01585"
     CDS             10660..12135
                     /locus_tag="PLS16_01585"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HNJ59797.1"
                     /translation="MKKVSVYVFISLLSAFLAVMIYGKLGNHSTIIKEESSGNANFKF
                     ASVTGGASQDAFIQAAKVSTPAVVHINTEVKVKQRKNMQMDPFWQFFGMPYDQGQMPD
                     QMQQGAGSGVIISNDGYIVTNNHVIEGADKINVNLNDNRNYTAKVIGSDPSTDLAVIK
                     IDAKDLSFLTFGNSDELEIGQWVLAVGNPFNLSSTVTAGIVSAKARNINILREKAGNL
                     AVESFIQTDAAVNPGNSGGALVDLSGNLIGINAAIATPTGSYAGYSFAIPSNLAKKVV
                     RDIIDFGVVQRGFLGVNIREVDEELAKDLKLDKIQGAYITDVVKGGAAEEAGLKNGDV
                     ITKVGGAEIKNIADLTEHVARYRPGDAVKFEYIRKGALQQKEIVLKSKDNSTKLLSKT
                     DVVTGNISADLGIEYSEVSSLQAKKMGLNGGVRIDKINEGIVKQNTNIQPGFVVFSIN
                     NQAVRTKEDFDSLIKENKGGGILLQGKYEGSSGVQYYAFGY"
     gene            12220..13200
                     /locus_tag="PLS16_01590"
     CDS             12220..13200
                     /locus_tag="PLS16_01590"
                     /inference="COORDINATES: protein motif:HMM:NF022732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HNJ59798.1"
                     /translation="MLKVLFFVVFLQSICFAQVSNYALEKNWAALPTIENASFWVPKN
                     ADLKNNQKEAEVDVFFIHPTTDIYGFKASGNTNIDNKKVNIKTDELSIKYQASVFNGT
                     CKVYAPRYRQAVLHNFFSKNSDKSKAAFNLAYSDIKAAFEYYLANYNHGRPIIIAGHS
                     QGTMHAARLLKEFFDGKPLQKQLVVAYLIGYPIYASEFQFIKVADDADSLGGFVSYNT
                     FLMGADNFFTEEYKNAVVVNPLSWKTDKQFVDATKHLGSIKTNHEQIFPKLFGAKCGN
                     GILEIQKPNVSGYVPLVKNNYHLHDYSLFYMNLRENIAHRANLYINLKKL"
     gene            13222..19326
                     /locus_tag="PLS16_01595"
     CDS             13222..19326
                     /locus_tag="PLS16_01595"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNJ59799.1"
                     /translation="MKKTLFITAMLFSFLTYAQNMNYKNDWDKISKLEGEGKTKDALD
                     LVSKILEQARKDQNIAQQIKSNLFVYKYKMILEEDSEWKIVQDIDNEIQKADVAEKAL
                     LHSISAELLNQYYSGNSWKFADRTKTEQKQSEDFRNWDLQTLYEKITYHYLASLENKI
                     VLQSIQLEDIKDIIITADKSKIFRPTLYDLLANRAIDFFSKDFADLTQPQNAFNINNS
                     EYFNEATSFVKQTISSEDTLSNAYWTMKIFQDLLAFRLQQNNPEALADADIKRLNYVK
                     EHYTDKDEANFLYYQALLNLNTQFAKSAYGDEIQYLLANFFNQNLDNQYLKNKIENKD
                     VLTTQNIMKICNDCIKNYPNSSGATNCLALIKLLQQKNIHITTEEVLLPNQQSLAKVT
                     YKNINKVYLKWIKVDYDKRDKLFELKNKETQDDIIKRLNELKVTKSWSQNLSQKIDYT
                     EHTSEIILPSVEHGFYVLMASTNSNFNLKNDGNAVVINQVFVSNIGYITKANSNENFE
                     IYVLDRKTSKPLQNATVTLFKREYNYQSGKYIKIKVTKLSTDSNGYAQYKNTLKPAET
                     YSSNYFLVEVNYKDDYLVGDETYYQSNYKTYEETPQKTVHFFTDRAIYRPSQIIYFKG
                     ILTEYYREENYILPNKPVTVVFRDANGQEISKQELTTNEFGSFTGIFTAPSNGLLGRM
                     SLETSYGSVGIQVEEYKRPRFEVLLDTIKTAYNLNDTIKVTGKAQSYSGANVDGAKVK
                     YTVTRVPNFPVWFGWRWFFPMPSTSDKIIATGATTTDEQGKYEFNFEAIPDLSLDKKY
                     KPYFNYKVNVEVTDINGETQTNHTEVRVGYLAIDASVNVPSYIDTKQANEISVNTNNL
                     NGQPEPTDVEIKIYSLKQPKNPLKSKLWTTVDEQLIAKTEFEKLFPYDVYNNENDARN
                     WEKGSLVKTYSFNTKDQKAILFKANEWEAGTYLVEFSCKDKNDNLIAFSNTFTAGTYA
                     DKLPQPPEFLSLQIDKSSLQPGDTLNYKISSSLKNVYVVLEVKHQNKWIDKKIILIEN
                     NTEFFKLPITEELRGGLQIRCYALLENRAFAQYGFVAVPYPSKDLKMEWLTFRDKLLP
                     GQKETWKLKISGNDKDKVVAELVTTLYDASLDAFVKHNFDFYLRGKTITNNLSAFQSN
                     DGYGVSSSSIYTSKKWNEYGKTIYQGYDALNLFGLQIAYPRYYLYRKGGGGIMMNAKS
                     GVMTESVTLEAPSLSPKEMDKNVSDDIAIEENYKDKVETTEPKPTEKVDVNPRTNLNE
                     TAFFIPQMQTDNNGNILLEFTMPEALTKWNLLGFAHTKDLYTTQFSESIITQKELMVV
                     PNAPRFLREGDTLYFSSKITNLSEKDLNGNVSLVIKDALTEREVNTEFSNKNHTQAFA
                     VKQGQNTAVFWKIIVPKGYSAVTYEVLAKAENFSDGEKNVLPVLSNRIIVTESLPLWI
                     SGKEKKKVTFNKLFNNKSKTLEHHALTLEMSSNPAWYAVQALPYIMEYPYECAEQLFS
                     RYYANSLASHIANSNPRIKSVFEEWKSTDSKALLSNLEKNQELKNVLLEESPWVREAQ
                     DETEQKKRIGLLFDLTKMAYEQQSALEKLTKLQSPNGGFMWFAGMPENRFITQYIVQG
                     IGHLNQLNVIDISKDKNLSQVVEKAITYLDGKMQEDFESIKKYDKDYLKTNHLGYDVI
                     QYLYMRSFFKENKIDKKYSEAYNYYLDQAKKYWNENKNLQMKALLSMILYRNNEFKLA
                     KTIIESFRQNAINSEEMGMYWKALDNPSWYWYEAPVETQALIIEAFNEITDDEKSIDA
                     MKMWLLKQKQTTNWKSTKATAEACYALLLSGTDWVSNDKQVEVKINNKILDTKQLDIE
                     AGTGYFKTKWFGEEIKPEMAKIELKKVDKGPAYGAMYWQYFEDLDKITGANTNLQLLK
                     KYFIKETTNKGTVLKEITANTPIKVGDLITVRIELKTDRNLEYVHLKDLRAAGTEPTN
                     VISRYKWQDGFGYYETTKDVATHFFIDWLAKGNYVFEYTLRASLAGNFSSGITQIECM
                     YAPEFKAHSQGERQIIEE"
     gene            complement(19332..20174)
                     /locus_tag="PLS16_01600"
     CDS             complement(19332..20174)
                     /locus_tag="PLS16_01600"
                     /inference="COORDINATES: protein motif:HMM:NF005881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ59800.1"
                     /translation="MNNFFQNKKCLITGAASGIGKAVALQLSKLGAVLILTDIQQEQL
                     ESTANEIKANDGNIAYLEALDITNFEKVKQFGEQVIQKVGNLDIVMNIAGIAIWGAVD
                     KMQHEEWKQVIDVNLMGPIHIIETFLPKMISEKNGGHLVNVASAAALFGLPWHGAYCA
                     SKYGLRGLSDVLRHDLKRHKIKVHLVCPGAVDTGLVSTIRISGLEITDEKFKTLKKQF
                     QRHAVSPENAAKSILKGVKNNDYYIFTSPDIKFGNWGKDKFTFASEWIMQIANDFFQK
                     TMKA"
     gene            complement(20181..20669)
                     /locus_tag="PLS16_01605"
     CDS             complement(20181..20669)
                     /locus_tag="PLS16_01605"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNJ59801.1"
                     /translation="MQISIIIAVSNNGVIGRDNQMPWHLSDDLKRFKKLTSSHCIVMG
                     RKTYDSIGKPLPNRTNIIISRNKDLTIEGVVICNNIEQAIDFAKAQNEKELFIIGGGE
                     ICRQALSITDTIYLTKVDTELEGDAFFSFDESKWQISEIEYIPKNEKNEFDSEFMILK
                     RK"
     gene            20811..22079
                     /locus_tag="PLS16_01610"
     CDS             20811..22079
                     /locus_tag="PLS16_01610"
                     /inference="COORDINATES: protein motif:HMM:TIGR03805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="parallel beta-helix domain-containing protein"
                     /protein_id="HNJ59802.1"
                     /translation="MKFLKVLAILFFAVGIFNKCKNNKEDSVKTEVLTDEAFQTKLQE
                     QLILVEDGDTIEIPEGKYSFTKTLSLEGKKNITIKGAGIDKTILSFINQTEGAEGINI
                     TNCDGITIELFTVQDAKGDNIKLKDCNNVIIRNMNSTWTTGADSTNGSYGYYPVLCKN
                     VLVENCEVSYCSDAGVYVGQSTNVIVRNCYAHHNVAGIEIENCINSDVYDNLAENNAG
                     GILIFDLPKLFQANGRNARIFNNKCLNNNFKNFAKPGAIVGSIPSGTGMIIMATDSAD
                     IYNNIFQDNKTVGVSIVSYHITGKPVNDSAYGAYSYAISVHDNQFSRKAVLIPDLKND
                     LGKLLLATAKSPVDIIYDGASDKSVRGKNGSLPEDKRICIRNNGDIKFMNLNAWKAEG
                     IVDLVKYYDMDITKFDCEIQGILGNNTPTK"
     gene            22082..23149
                     /locus_tag="PLS16_01615"
     CDS             22082..23149
                     /locus_tag="PLS16_01615"
                     /inference="COORDINATES: protein motif:HMM:TIGR03806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SO2930 family diheme c-type cytochrome"
                     /protein_id="HNJ59803.1"
                     /translation="MNKTKFIFFLIVCLLIFAQCKTKSKTVNLYQDGTPFQKLSEYNF
                     FKGKISDLIPNESVLPYDVATPLFTDYAEKARFVWLPEGTSANYNKDEALDFPEGTIL
                     IKNFYYWNDFRDHSKGKRIIETRLLVKEKKGWNTNNYLWNDAQTDATLDVVGKTIPIK
                     WINEKGEQMGTNYSVPNKNQCKNCHNFNNTFMPIGPKVRNLNHDFTYQEGTKNQMEKW
                     TEVGYLKGFVAADNIHNKLPKWNDQKSGTLEQRAKAYLESNCAHCHRPEGNANTSGLF
                     LLTTDHNPESWGIMKSPVAAGKGSGNRLFDIVPGKSDESILTFRMESLDVDAMMPELG
                     RTMVHKEGVQLIKDWIASMPQ"
     gene            23197..23736
                     /locus_tag="PLS16_01620"
     CDS             23197..23736
                     /locus_tag="PLS16_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59804.1"
                     /translation="MIRTNRLSSLLFIFIISFVFSACKYSDKPKTFNNGAVSLEYPSY
                     LKSSKDIYPVENLLIGVKNDYRNVFFILLDYGQKPGEQGFELMYDSLSTQLKRGIREP
                     FVEKDSSFNINGFKVREMQVSGILASVGQEKRMLFTFDLFEDKNGHLYQTAGWCFRHR
                     RDLWEKDLQQIAYSLKSLK"
     gene            complement(23737..25965)
                     /locus_tag="PLS16_01625"
     CDS             complement(23737..25965)
                     /locus_tag="PLS16_01625"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNJ59805.1"
                     /translation="MELSSEAEELERKEILKDYKQLFRLLRPRMQAGDDKRVRRAFEM
                     AVEAHRHQRRKSGEPYIHHPIAVAKITCGDIGLGVNSVICALLHDTVEDTDVTIDDIK
                     KEFGPVIAKIIDGLTKISKISDQTESIQAENYRKILLTLSDDIRVILIKLADRLHNMR
                     TLNDVSKKNQLKISSETLYLYAPLAHRFGLYSIKSEMEDLAMKYLEPEKYKEIARLLD
                     ETKKERTRFINDFVKPLQDEMDRIGIKARVFGRPKSISSIAHKMKKKGVSFDDVYDKF
                     AIRIIIDAPPEKEKADCWQAYSIVTDKYTPQPNRLRDWLSHPKANGYESLHTTVMGPG
                     GNFVEVQIRSERMDEIAEKGLAAHYKYKENNTAQKQVHTAEEAIETWLMQVREFLKNP
                     ETNPVEFINDFKLELYNKEIYVFTPKGEMRVLPVGATTLDFAYAIHTNVGDQTIGAKV
                     NNKLVPISFKLSNGDQVEIITSKKQKPTEDWINIVTTSRARQKIKNSLKEEKKKIAEH
                     GKEIVERKLSHLKCSLTNENINVLCQFYKSPSSLDLFYDIGREAITLNNIKQLKVVGG
                     NLEIPKIEKEKKEEVFEAPKSNFSNNSELVIFGESSSQFPYELAKCCSPIPGDDIMGF
                     ISINGVIKIHRTGCNNIINLASRYGYRIIKTSWTSKAAPSFLTTIKLNGLDHIGIMNK
                     LTHIISQEMKLNMQSISLESIDGMFEGIIKIYVKDNNQLNKLMEKLNATEGIHQVIRE
                     EF"
     gene            complement(26043..26645)
                     /locus_tag="PLS16_01630"
     CDS             complement(26043..26645)
                     /locus_tag="PLS16_01630"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNJ59806.1"
                     /translation="MKNLSLILNAVLFILVGILFYLHFNGKKSAAPVTVKSLDGKTQA
                     VQGQVIAYFDIDSFQNNYTYYKQKKHEIETKQNAIESEMARDEKKIQSMIEGYQKKAE
                     TMTEEEYYIAQQKLAVEQQKAQEKVQNSTDALMKMTEKFNLELMESITVYLKEYNADN
                     RYTYILPYTKSNPNLLYVDERFDITKDIIEGMNAKLKQKK"
     gene            26797..27462
                     /locus_tag="PLS16_01635"
     CDS             26797..27462
                     /locus_tag="PLS16_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M10"
                     /protein_id="HNJ59807.1"
                     /translation="MCLIKIDKSNNVISINSVFNIYGTYANEHYANCIKNEIETMWNA
                     PKGIVQLYNQSFNVCFKVDVVIYDLISPTSILENDNFLNKYIRIEEVSKMQVSFVDGI
                     NSSTGYFTINNLEQGTTVAHEFGHMLGLKHPENLDYRGKGRPHIMYPRGTLVDAEFQY
                     DANAIAGQPGGTVNPTVRVVTQENIDDLKLGNIIEQGQAHIGKISTNYHDIFIKSAPT
                     QSA"
     gene            27630..28079
                     /gene="umuD"
                     /locus_tag="PLS16_01640"
     CDS             27630..28079
                     /gene="umuD"
                     /locus_tag="PLS16_01640"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF007621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HNJ59808.1"
                     /translation="MTPLKLHTGSIIDIFSANTETKLALPYVEEGISAGFPSPALDFV
                     DLTIDLNRHLIKNPSATFYGKVKGDSLKNAGINNGDLLVIDRSLDPINGKIAVCFIDG
                     EFTAKRIQILKNEIWLVPENENYPSIQITEENNFIIWGIVTHAIKNL"
     gene            28080..29339
                     /locus_tag="PLS16_01645"
     CDS             28080..29339
                     /locus_tag="PLS16_01645"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HNJ59809.1"
                     /translation="MFALIDCNNFYASCERVFRPDLNGKPVVVLSNNDGCVIARSNEA
                     KALGIPMGAPAFEYEQLFQKEAVHVFSTNFALYGDMSNRVMSILSDYAPEIEIYSIDE
                     AFLKMDGFDLFNLQTYGEEMRHKVTKCTGIPISVGIAPTKALAKVANRIAKKYPKQTN
                     GVYIIDNEEKRIKALKWLKVEDVWGIGRQHANRLNAMGVKTAWDFTLLDDGIVRKQMS
                     VVGLRLKHDLQGIPTLDLEAIQIKKNIATTRTFETHYTEYGQLAERITTFASSCAEKL
                     RKQNSCCNSLMVFIQTNMHRNDLAQYNRSMVLKLPFPTNSSIELAKFAKQALQYIFRQ
                     GYHYKRAGVVVQDFSQGNQLQQTLFEQRDERHIRLMQAIDQLNNRLGQQKIRLASQDI
                     KRVWKMKQEKRSPQYTTKLNDIITIKV"
     gene            29732..30778
                     /locus_tag="PLS16_01650"
     CDS             29732..30778
                     /locus_tag="PLS16_01650"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNJ59810.1"
                     /translation="MKEIVSQKIRQGRVLNAMSLQELADLIGVSKQMISKYENGTSMP
                     SSKILIQLAKSLNVKLDYFFAPSSVELGNVNFRKRSSFSVKKINALKEDIKIQLSNYL
                     EIENILKIDNSFENPLKKKKVNSLEDIEKVVRELRNDWEIGFDPIHNIIQLLEDNEIK
                     VIEIDEPQNNFDGLAAIVNDKYPVIVVNKNFIVERKRFTLLHELGHLLLDLPECDMKF
                     EEQICNRFASEFLFPKSMVLKEFGEGRGSISFKELIEVQRKYGISIKAIIYRLKDCGI
                     LSENRVAEFYRKINFNPRLKDEVDAQRFQTPEFSNRFEQLVYRAYSQELISSSKAASL
                     LNININEVLENSTM"
     gene            30784..31299
                     /locus_tag="PLS16_01655"
     CDS             30784..31299
                     /locus_tag="PLS16_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59811.1"
                     /translation="MRIVINDANILIDLFHLDLVEVFFSLQGFEVKTTDFVFEELYDE
                     QKNIIKQFIQNQSLILIESSEDDLINIFEIFSSTKGLSVEDCSVWYHAKKNKGILLTG
                     DGNLRKQSLADGVEVRGILYIFDQLLISGLISFEFAIEKLNQLYSINERLPIDAKKQR
                     LDCWSRSEHIA"
     gene            complement(31488..34799)
                     /locus_tag="PLS16_01660"
     CDS             complement(31488..34799)
                     /locus_tag="PLS16_01660"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HNJ59812.1"
                     /translation="MKGKYYESDYEEALIQLLTQQGWAYTHGGSIARNNREVLLVDDL
                     TQYLQQGYPDLQANDIEEIINKLRHVSGQTQFELLRNTYYLLRDGFRYIRSQDGASFD
                     IELINFEPQNNNNIYRCVNQFEVGYGLKDDVRIPDVLLFVNGIPLCIFELKNPTDFNA
                     SIANAYEQIHTRYKRDIPHLLCYCPLSCISDASINNTKLGTTYTPYNHYYAWKKVNNE
                     DQSAQRGIDQVKTIVAGVYEPNRFLEILRDYVYFPDKNHDKEEEIICRYPQFFATRML
                     RESVLTAHEANSRKGGTYFGATGCGKTYTMLFLARQLSLRCTELGSPTIVMIVDRDDL
                     QTQAGKLFLRSEEFLSLGAAKIIADRKDLKTELSIRESGGFFICTIQKFCEEIGELNT
                     RKNIICFSDEAHRTQVKLNKQLKIKDQKSLKAQNENGEILEVEGLGAFITKPYAEHLR
                     AAFPNATFVGFTGTPIDETIQVFGDVVDSYTMQQSVEDEITVELKYIPRIANVTLDSE
                     KVKQIDDFYKKCADEGATEDDIAASKTAMSAMEVILGDEERLDRLAKDIIAHYSNACE
                     NKADVVQKAMIVCSKRKIAYRLLQKFKTHKPEWFEEKKSPDDSKLTAEELRELSPMPT
                     IAMVATRGANDPEDMYDYLGDKARHKKLDDAFKTEQSNFRIVIVVDMWITGFDVPSLT
                     YLYNDKPLQKQTLIQTISRVNRKYEGKEYGYIIDYIGIRDNMMEAMRKFGGETFGPSE
                     DDVKQALEALIIELQILKDVFVDFDITPFTDPKATPLERLECLSAAADYIITLTETLN
                     LSDEKEKPKAVGVKTFFLAHVKRLRTAYDICQPSNVLDNERLSLSQCFMAVASYIRKT
                     SGEKHDTESMNRAVEKMVAEALKCNAVVSILQTDVEENIFSPEFVEQLDSIKLPATKL
                     EVLIKMLRKSIKEYKDTNKIAAEKYEELLNKTLEEYHNRRASLSSSEASETQAEAVDT
                     IIRNATQQALDILSKLGEDKDSFRKLGLTFEEKAFYDILMHLRDKYNFEYGEDKKVGG
                     LIINDKCKVLAQKIKELIDVQSSFTDWLNNSNVRADLNQKIFFCLHNSGYPPQYNDEI
                     FDQVMGQVENFKHKN"
     gene            complement(34828..>35506)
                     /locus_tag="PLS16_01665"
     CDS             complement(34828..>35506)
                     /locus_tag="PLS16_01665"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNJ59813.1"
                     /translation="QQELVDTYNGLKALAEQNEALIQPLTQACQAYIVDCKKKYPEVQ
                     LGEYIELSDERNNDLKFTLDDVKGISILKKLIPTKADMQDVSLSPYKLLKPKDFSYVT
                     VTSRNGGKISLAINDTEETFIVSSSYLVFRTINAEKLLPEFIFLLLSREEFDRYSRFN
                     SWGSARETFDWNELCRTKIPLPPLEVQQAIVNLYHCAEEAKNIAAEAREKMKTLCPAL
                     VQKAMHG"
BASE COUNT        12183 a         5422 c         5878 g        12023 t
ORIGIN      
        1 attataacct ttctctttca ataattcagt taaatcttcg tcactttttg gtttggattt
       61 ctcttcattt tcaataatat cagagagtat tttttttact tctcttgttg aaacttcctc
      121 accactatct gtttgcaatg attctgagaa aaagtatttt agtaagaaaa taccaaattc
      181 tgtttgtaca tatttgctgt tagctactct cgaaacggta gaaatatcta atcctgtaat
      241 ttcagcaata tcttttaaaa tcataggacg caaatccatt tcatcgcccg aaaaaaagta
      301 cttttcttga aactggacaa tggcttgcat cgtagcaaaa agtgtgtgtt gcctttgttt
      361 tatcgcatcg ataaaccact tggcagcatc aatcttttgt ttgatgaaag ttacagcttc
      421 tttgtttttc ttatctttta cattgccttt tccataagaa accaacatct ctttaaaaga
      481 atcagatatg cgtaaatcag gtgcattttt accattcaaa taaatctcta attttcctcc
      541 aatttcttct actgtaaaat caggaataat agtttgatga gagatagaag ccccactatt
      601 gctattcgtt ccgcctggct ttggatttag ttgtataatt tcgtctaaaa ctgctttaag
      661 ttcgtcttcg ctaatgttta atgctttggc taatttatcg tagtgttttt ttgtaaatgc
      721 atcaaagtat ttatttaaaa cttcagttgc aagttctact gtttttgttt gtttttttct
      781 ttctaattgt aaaatcaaac attcttgtaa acttctagct cctatcccag cagggtcaaa
      841 gcgttggata agactgagta tgtattctac ttctttttcg tctgtgctaa tatttctttg
      901 aaatgctaaa tcatcaacaa tggcttctaa ctcacgtctt aaataaccat catcatctaa
      961 acttccaatt aaatggtcag cgattaaata ttcgtgatcg cttaattcat tttgcaaggc
     1021 taattgttct aatagatagt catgaaaaga ctttccgaca gttaaaggca tttgcttttt
     1081 ttcttcttgt cttccagtat agtcatcatc tgcattataa tcgtagtcat ctttagtata
     1141 ttcattgata tctaaatcgc taccaaattc atcatcttca ttactttgat tttcataatc
     1201 atcttcctgt tccttgattt cttgaggctc tgctaaatct tctttttggt tgtcaaaatc
     1261 atttggattt tcttccaatg caggattgat ttccatttct tctttaatgc gttgttctaa
     1321 taaagaagtt ggtatttgca ataacttcat tagctgaatc tgttgaggag acagcttttg
     1381 caacattttt tgtgataatc tttgtcctaa catctatact tgtatcacaa caaaaataca
     1441 aaaaataagc caaaataaac tatcttaatt caaatatgca attgcatttg aagtgttttt
     1501 taatgattta aagtacactg ctcaagttca cacataaaaa aaacccgacg aagtcggact
     1561 tttttctata attaaattgg attatccaat tagtccattt tttaataaaa attctccaat
     1621 ttggatagtg ttggtagctg ctccttttct taaattatct gcaacaatcc ataagtttaa
     1681 agtttttggt tgagattcat ctcttctgat tcttccaaca aaaacttcat cttttttatg
     1741 tgcattcatc ggcattggat aaatttggtt agcatcatca ttttctacaa taataccttc
     1801 cgttttactt aaaatatcaa tgacctcttt tacatcaaat tcattttcaa actcaatatt
     1861 tacactttca ctatgtccac ccattactgg aatcctcaca gttgtagcag taactttgat
     1921 actatcatct tgcataattt tgcaagtttc ttgtatcatt ttcatctcct cttttgtgta
     1981 accattttct aaaaaaacat caatttgagg aatgacattt aagtcaattt tatatttata
     2041 agccatttca tcagagctac cagttgctct ttcattcatt aattggtcaa ccgctttttt
     2101 gccagttcct gtaacacttt gataagttga aacaacaact ctatttattt tatattttaa
     2161 gtgcaaagga ttcaaaactg ccaccatttg gatagtagaa cagtttggat tagcaataat
     2221 tttatcttct tttgttaaag tcgcaccatt aatttcaggc acaactaatt ttttagtagg
     2281 gtccattctc caagcagatg aattatctac caccgtgata cctgcttcag caaattttgg
     2341 ggcccattct tttgaagtat ctccacctgc tgaaaagaag gctacatctg gttttagtgc
     2401 aatagcttgt tgtggtgtta ccacggtata ttcgtttccc ttaaattgta tttttttacc
     2461 tgctgatttt tctgacgcaa ccggaactaa ttctgttacc ggaaaatttc tttcttctag
     2521 tacttgtaac atttcgctac caactaatcc tgtagagcct acaactgcaa ctttcatata
     2581 tttctaattt aattaagcta aattaccatt ttcgagagga aatcttttta aattaatatt
     2641 attaattagg cgttttatac ttaaaataaa taaattatga ctgaactaat ttctgttaaa
     2701 taagcttact attcagactg atgcataata tttagtacat ttgtatttct atcaacctat
     2761 gaaaatgaat agatttatct tgcacctaaa tattcttata ttggctattg tagttttggt
     2821 acaatgtaaa actgcaaaga cggaagacaa cgttaatgct ataattaatt cagaagagat
     2881 ttctgataag gattttaatg aacaaataag tgttttgata aaaagcgaaa ttgaaacatt
     2941 agcgaacaat aaagtggata ctgctaattt tacctctttg caaactatac ttcatcgtgt
     3001 ctataaatct acagacttcc agccgatttg ggttaataag cacatattaa atgaaaatgg
     3061 caaatctctt ttttattttt tagcaaatgc tgaattgcat gcattaatta aagaaaaata
     3121 taattatgaa atcataaaac aactaaaaga ttctttagag cacaatctgc cgtctatcca
     3181 atttctttta attaaaaaat tagatattga gctaagtaaa gcatttttgc agatggcttt
     3241 acatattgat aaaggtattt ataatgagca gaaaaaggta attaataaga atttctttga
     3301 aaaagaagat acttacactc aactgttagc ttcaataatt gctggcaaaa atcctgaaca
     3361 ggctttagtg tcattagagc cgcaacaccc gatgtataaa cgccttaaag cggctgttta
     3421 tcagtttgta tctaaaagta atatcagccc tcaaaaaatt agtattcgca attataaaac
     3481 agattctatt ggtgctatca atgatgcaaa attagctttg acttatcatc attatttgca
     3541 tgatacctta atgaatgata atgttgccta tttatcggca ttgaaagcat ttcaaaaaga
     3601 ccaaggttat agtcaagatg gtgtagtagg caatatgaca gctcgtgctt tagaaagaga
     3661 taatagccaa aaatttagat tgctgttgat taatgttgat aggtggagaa gtaagaaaaa
     3721 cgatattaaa atagaaccaa aatatgtttg ggtgaatttg ccttcttaca agttaagtat
     3781 aattcgtaat gattctctgt tgttacagaa gaatgttgtg ataggaaaag ctggtggcaa
     3841 aacagaatct ccagaaatta taagtgcgat taatcaaatt gtagtttggc caacttggtc
     3901 ggttcctcaa tctatcatca agaatgagat gaaaagtttt aaaggttata ttgtttctaa
     3961 aaaaaatggt tatacgtcag tagttcaacc tccaggactt aacaattcat tgggagcagt
     4021 taaaattctt tttcctaata aacattctgt atatattcat gacacacctt caaaatattt
     4081 gtttaagagt gattatcgtt cacatagcca tggttgtatt cgttgtcaag acccatttga
     4141 agtggcagga atgttgttaa atatggataa tccagcgata gattatgatt ccttaatcag
     4201 attgaaagaa aaaaagattg caacaaaaaa tattagttta aaaaatccta ttcctgtttt
     4261 tttgcaatat tttacggctg aagcagattg gaatggaaac ttaaaattct atgcagatac
     4321 ttataaaaat gatagtgtta ttatagctac tatttttgga aaaatagata atacaacaaa
     4381 accaataacg atacagaaaa tcaagattga tagcatctta aatactacta atcgtccatt
     4441 gaaaaaggat tctacaatat taaacaaatc agatagttta ccaaagaaag aagatgagat
     4501 tattttactt aaaaatgata gttttgaaaa atctagtact gaaccttttt agtctttaca
     4561 atagtttgat tataatatta ttattaaaat attgaacatt atttttatca cattattgta
     4621 atttttttgt ttcatataat ataattttga tttaatttta aaacatcatc ttttaaatta
     4681 aaaatagttt taatatggaa aatcgaacaa aaattaagtc agtaataatt ttattattgc
     4741 taacaatatt aaattcatca atattacttg cacaatgggg aactttaggt aatcagatta
     4801 aaaatggatt gagcaataaa gcacaacaaa agataaaaca ggaaataaat gatggtgtag
     4861 ataaggctta tgataaatcc aaacaaaaaa ctaaagagag tgtaaaaaat agcaaagaaa
     4921 aagatgatga gagcaatagt aattcttctt ctgatgtttc taacgaaaat aataaaaata
     4981 gttcgtcaaa ttcaacagcc aactctagtt taaaagcgta tggaaaattt gatttcattc
     5041 ctggagaaaa agttatagca caagaggatt ttacgcaaga tgcaattgga gattttcctt
     5101 ctaaatggaa tacaaatggt tctggcgaat tggttacgat tgatggacaa caaggaaaat
     5161 ggttaaagtt tgcacctcaa agtattatct atcctgaatt tgttaccaat cttccagaaa
     5221 attttacagt agagtttatg ttggcaagta cacaaccatt tagtttttat tcttctgctt
     5281 ttaacttctt ttttgcacct ttagtaagtc catcaaagaa ttatgtacaa tggaaacaat
     5341 atagcggagg tagaaaagat ggtgttttag ttgcaattca tcctgaagct gctggtggta
     5401 atgctatggg acaaaaatgg attacaactt atgatgaaac tggagcagaa attgttaaga
     5461 atcaatcaga ctttccagat tttaatcatg aaaataagaa tgttgtaaaa gtatctattt
     5521 ggcgacaaaa acaacgctta cgagtttata taaacgaaac aaaaatctgg gatattccga
     5581 aagcatttag tgcgacagca aaatataatt ttatgggatt tagaacagac agttatcata
     5641 atgacaatga tgcctatttt ttaagtaatt tacgtgtagc tgttggagct ccagatactc
     5701 gacataagtt tttagaatta ggcaaatact caactactgg aataaaattt gatgtcaata
     5761 gtgataaaat aaaaggcgaa tcttatggaa caattaaaga ttttgctgat gtactcaaag
     5821 aaaaccctga tgtgaaagtt aaaattgtag gacatacaga tagcgatggt gatgatgcta
     5881 gtaatttggc attatctaag aaaagagcag aagctgtgaa agctatctta aacaaagagt
     5941 ttggtattga attaagcagg atggaaactg aagggaaagg agaattgcag cctacagaca
     6001 cgaatgcaac tgctgaaggt aaggcaaata atagacgtgt agaatttatt aaactttaga
     6061 cagacaagct tattttagta atttggtttt ggttaatgat ggaattcttc atagaaatca
     6121 actttcccag tttctatatc atacatcgca ccaataattc ctatctctcc agatgcaatc
     6181 atttgttcta aaataaagct tcggtcaagt atggcttgta cactttttct tacattgatt
     6241 gcagatacat tattgacaaa ttgtagattt tttgagtttc tcagttctgt tggtgttgtt
     6301 ttttcagcat aaactgctgg ttgaattttg gttaataatt cagttaaact tcctagctct
     6361 acgaaatcac aagcaccttt gatggcacca catttagtat gtccaagtac tactattagt
     6421 tttgaatttg ctaatttgca tgcatattcc atactgccaa ttacatcagt attgataaca
     6481 tttccagcta ttctaactga aaatatatca cctaagcctt ggtcgaaaat caactcagca
     6541 gaagtcctac tatcaataca acttaaaatt attgcaaaag gcgattgtcc atctctggtg
     6601 gcgtttactt gttcaagtaa attgcggtgt gcttctaggt tattgacaaa tcgttcattg
     6661 ccatttttta ggatttggag tgccatttct ggtgttaaat gttcctttgc acttttgtct
     6721 aatgttttca aattgtaaga gagattaaat tgcttgagaa tgaatttaac aaatgtacaa
     6781 tttgatttta ttaaatttta atttgttgta atattttaaa aataaaaaag gtcattcaag
     6841 agaatgaccc tatttatata taaagttttt gtttattaga acttagcaat aaaatataac
     6901 tgagcaccag tgtttaaagt ttttttatct ggtggcgtta atggtgcagt gaaaaatttc
     6961 ttattcatta catttataaa tccttggttt aacttaaaac cagcaccaaa tcctttttga
     7021 gaaataaatt caagtcctaa attaacacca gcatcaattg gattgaataa tttttcctta
     7081 tttgctgggt ttgtaggaat atcttctttt ttgtttttaa catcaggtgt gcctggatta
     7141 acaaaatcta catttgtaac ttgttgcatt ttaaatgcat aagagaaaaa gccaccccca
     7201 ttcacattaa atattttttt ctttccgata taaaacttta caattaaagg aatatcaatt
     7261 gaatggaagt tataagtaga gcttccgcta ttaattaaaa ggttcttttt taatcgatta
     7321 gcagctcctt tgttcacata acttacgcca gtttctaaag aaagcctgtc tgctaatcta
     7381 ccctcaaaag taaaaccagc aaatcctcct acttttgttc tgtttttagc ttcataactt
     7441 ttctcgcctt cagtaaatgc tgagttagca cttgttccaa tattaaaccc aatcataatt
     7501 tttttgtcag tgattggttc ttttgcaaat gtgctgatgc taactagtat acccaacact
     7561 aaaattattt ttttcatata gttgttttta attaaaaaaa tagtttggca aaaataatgc
     7621 cattaataag attcttttac cactaaatat aggtattttt tctacatatc tattgtgtca
     7681 attgaatttt gtacattcac atcatattat gcaacaagat tattttgcta ttggattaat
     7741 gagtgggagt tccttagatg gattagattt gtgttatgtg catttttctc gcaaagataa
     7801 tttatggttt tcttatacga ttttagctac tgattgtatt tcttataatg aagcgtttag
     7861 agaaatacta aaacaagcac caaacttgtc tgcaataaat tttgcaaaac tacatattga
     7921 tttaggtcgt ttttttggaa atactacatt agattttatt caaagacatc aaatagaaaa
     7981 aatagatttt attgcttctc atggacaaac catttttcat caacctcagc agcattttag
     8041 tacgcaaata ggttgtggtg caactattgc acaagttacc caagttaaaa ctatttcaga
     8101 tttaagaatg agtgatgtgg cggcaggtgg acaaggtgct cctattgtac ccattgcgga
     8161 gaagtatttg tttccaaatt atgatatatt tctaaatatt ggaggtattt gcaatattgc
     8221 cttccaccat aaaacagcca atcatatttt agcgtatgat gtttgtgcag gaaatacatt
     8281 gctcaattat cttgctcaac aaaaaagcaa aacttttgat gaaggtggtc aaattgctca
     8341 acaaggaaat gtttgtgcag aacttttaca tcagttaaat tctatacctt tttgctcgga
     8401 gaaatcacca aaatcattag gttctgaaca tatttatcaa gattggattg cattaactga
     8461 tatttccaat atctctattg aagataaaat ggcgacttgt gtcgaacata ttgctcaaca
     8521 aatcggaaat caaattaatc agtattctac atcaaagtct caacaacttt tagtttctgg
     8581 cggtggtgct tttaatactt ttttaattca aagaattcaa aattatacat ctataaaagt
     8641 tgtagttcct gataaagcaa cgatagagca aaaagaagca ttagctatcg ccttttttgg
     8701 attgctacga atattacaaa ttccaaattg tatttcgagt gttactggtg ctaataaaaa
     8761 tgtggtaggc ggagctgttt acttgcctta atttataacc actttttatg tttaaaataa
     8821 tacaaagata cgcctgtaat caatgctgaa attatagtta caattgaaaa agcaaatgga
     8881 tgttcttgaa atgggagtgg aatattcata ccgtaaattc cggcaatcaa agtgggaatc
     8941 attaagacaa tagtaacgcc agttaaacgg tttaccacat aggctaaatt attagaaata
     9001 atagaaccaa aggcactcat catatttcct aaaatacctg aataaacatt agacatatct
     9061 attgcctgac tgtattctat cattacatct ctaattattt tattgttctc tatagaatca
     9121 ttttgaatag aaagtgcttc tttatgtttt tctaaaatca attcatctgc tcgtaaatca
     9181 ttcagaaagt aaattaagga tttttgaatt tttaaaagtt tatttaagcc atcgttagaa
     9241 ctcgaatgtt ttaaattctt ttcaatttta gaaatctgaa cattaatttc tcttagataa
     9301 aaaataaaat aaaaaacagt tcttttgaaa atatataata ctactgtatc caaagtaaat
     9361 tccagctgtt tttctaacat attttttgta caccaatcca tcattggatt atttattgca
     9421 ctaaacgtaa aaagtttctc ttttgtctga attatcccaa caggaattgt caaataatca
     9481 gcttcatcat cgacatcaac tgcattatta taaattggtg tgttaatgat aatttgtttt
     9541 agttcctttc caatttcata tcgtgattgt tcatataaat ccatacaatc tgtaaaatgt
     9601 gaattgggaa tattattttc tagggcaaat tgctccaaat ctttggctac aaatggcggt
     9661 gtaatatgta tccagttcgc atttgatgca tcatcaattt gcaagagttt tccgttttca
     9721 attttaaagt ataaaaacat cttagatttt tatttcgcct ttaaatacaa attcagcagg
     9781 accttctaac caaatatccg tgtattgatt gtcttgcttg gtaaaataaa catttaagtt
     9841 gccacctttg gtaattaaat tgatggaacc agcatcaata gatttaattt gtgctacaca
     9901 aagagcagca gccacatttc cagttccaca agaaagtgtt tcatcttcaa cgccacgctc
     9961 ataagtgcga atcttgactt catcatcaat agaaatgtaa ttaacattga taccgtcttt
    10021 cttgaaatca tcattatatc taatactttt ggcatcttct acaaaattat ccatttctac
    10081 gttaggtaca attttaatat aatgcggcga accagtattt aaaacaaagt cattttgata
    10141 agcattgatt ttttctacat ctatcatttt tagacgaatc aaattgtttt tatataaaaa
    10201 ctcatgtttg ccatcaactg caataaattc agcttctttt ttagtaacaa tacctaagtc
    10261 aatggcaaat tttgaaattg ctcttccgcc atttccacac atactgcctt ctttcccatc
    10321 tgaattgtaa taaaccattc tgaaatcata tccttcagca ttttctagta acatcaaacc
    10381 atctgcacct attccaaatt ttctgtcgca taactttgct atcaaattat gattttcttt
    10441 aggaaaaaat aaagttcgat tatctactag cacaaaatca tttccagtac cttgatattt
    10501 ataaaattcc aaattcatat tggtaaaaat aagagaaaat taaatgattt gtatctctta
    10561 atttcgatta acttttttta accgctattt gtgttttatt agtacttaac aaaaaacatc
    10621 tgcactaaat ttgtagttag atatcaaaag attcaaagta tgaaaaaagt tagtgtttat
    10681 gtttttattt ctttattgtc tgctttttta gcagtaatga tttatggtaa actgggcaat
    10741 cattccacca tcataaaaga ggaaagttcg ggtaatgcca attttaagtt tgcaagtgta
    10801 acgggtggtg catcacaaga tgcattcatt caggctgcaa aagttagtac accagcagtc
    10861 gtccatatca atactgaagt gaaagtcaaa caaagaaaaa atatgcaaat ggatccattt
    10921 tggcagtttt ttggaatgcc ttatgaccaa ggacaaatgc ccgaccaaat gcaacaagga
    10981 gctggttctg gtgtcataat ttcaaatgat ggttatattg taacaaacaa ccatgtaata
    11041 gaaggtgcgg ataaaataaa cgtcaatttg aatgataata gaaactatac agcaaaagta
    11101 attggctctg acccttcaac ggatttagca gttattaaaa ttgatgctaa agatttatcc
    11161 tttttgactt ttgggaactc cgatgaatta gaaattggac aatgggtctt ggcggttggg
    11221 aatcctttta atttatcttc aacagttact gctggaattg tgagtgcaaa ggcaagaaat
    11281 attaatatat taagagagaa agctggaaac ttagccgtag aatcatttat ccaaacagat
    11341 gcagctgtaa atcctggaaa tagtggtgga gcattggtgg atttgagtgg caacttaatt
    11401 ggcattaacg ctgcaattgc tacaccaaca ggaagttatg ctggttattc ctttgcaata
    11461 ccttcaaact tagccaaaaa agtagttaga gatattatag attttggagt agtgcaacgt
    11521 ggttttttag gtgtaaatat tcgtgaagta gatgaagaat tagcgaaaga cttaaaatta
    11581 gataaaattc aaggtgctta tattacagat gttgtcaaag gtggtgcagc tgaagaagct
    11641 ggattaaaaa atggagatgt cattacaaaa gttggtggtg ctgaaattaa aaacattgcc
    11701 gatttaacag aacatgttgc aagatataga cctggagatg ccgtaaaatt tgagtacatc
    11761 agaaagggtg ctttgcaaca aaaggaaatt gtattgaaat caaaagataa ttctactaaa
    11821 ttattgagta aaacagatgt tgtaacgggc aatattagtg cagatttagg catagaatat
    11881 tcagaggtat cttcgctaca agctaaaaag atgggtttga atggtggagt aagaatcgat
    11941 aaaatcaatg aaggaatagt gaagcaaaat acaaatattc agcctggatt tgttgtgttt
    12001 tctataaata atcaagcagt acgaactaaa gaagattttg actcattgat taaagaaaat
    12061 aaaggcggag gtattctatt acaagggaaa tatgaaggct ctagtggtgt tcaatattat
    12121 gcttttggct actaatagat tttagataat agatttaata atgtttattt gtaaaatatt
    12181 tcattaactt tattagagct aaataatata aaatattaag tgttgaaagt attgtttttt
    12241 gtcgtttttt tgcaaagtat ttgttttgct caagtaagta attatgcctt agaaaaaaat
    12301 tgggctgcct tgccaactat tgaaaatgca tcattttggg tgccaaaaaa tgctgattta
    12361 aagaataatc aaaaggaggc tgaagttgat gtttttttta ttcatccaac aacggatatt
    12421 tatggattta aagcttctgg aaacacaaat attgacaata aaaaagtaaa tattaaaaca
    12481 gatgagctta gtataaagta tcaggcgagt gtttttaatg gtacttgtaa agtttatgca
    12541 ccaagatatc gtcaagcagt attgcataat ttcttttcta aaaatagcga caaatcaaag
    12601 gcggcattta atttggctta tagtgatatt aaagcagctt ttgagtatta tttggcaaat
    12661 tataatcacg gaagacctat tattattgcg ggtcattcgc aaggcacaat gcatgccgct
    12721 cgtttgttaa aagagttttt tgatggcaaa cccttacaaa agcaactagt agtagcttat
    12781 ttaattggat acccaattta tgcaagtgaa tttcaattca taaaagtggc tgatgatgct
    12841 gatagtcttg gcggatttgt gtcgtacaac acatttttga tgggagcaga taattttttt
    12901 actgaagaat ataaaaatgc ggttgttgta aatccattga gttggaaaac agacaagcaa
    12961 tttgtagatg caactaagca tttaggaagt atcaaaacaa atcacgaaca aatttttcct
    13021 aagttatttg gtgcgaaatg tggcaatgga attttagaaa ttcagaaacc aaatgtctca
    13081 ggatatgtgc ctttagttaa aaataattat catttacatg attattcctt attttatatg
    13141 aatttgagag aaaacatagc acatcgtgct aatttatata taaatttgaa gaaactataa
    13201 gctattcatt aaatcacttt tatgaaaaaa acactattta ttacagccat gctgttttca
    13261 ttcctgactt atgctcaaaa tatgaactat aaaaatgatt gggataaaat atccaaatta
    13321 gaaggcgaag gtaaaactaa agatgctttg gatttggtgt ctaaaatctt ggaacaagct
    13381 agaaaagacc aaaatattgc acaacaaata aagtccaatc tatttgttta taaatacaaa
    13441 atgattttgg aagaagattc tgaatggaaa attgttcaag atatagataa tgaaattcag
    13501 aaagccgatg ttgcagaaaa agctttattg catagtattt cagcagaact attgaatcaa
    13561 tattatagtg gcaactcttg gaaatttgcg gatagaacaa aaaccgaaca aaaacaatca
    13621 gaggatttta gaaattggga tttgcaaacc ttatatgaaa aaataaccta tcattatttg
    13681 gcttctttag aaaataaaat tgtattgcaa agtattcaat tagaagatat aaaagatatc
    13741 attattactg cggataaatc caaaatattt cgaccaactt tgtatgattt attggccaat
    13801 cgagcaatag atttttttag taaagatttt gcagatttga cacagccaca aaatgcattt
    13861 aatattaata attcggaata ttttaatgaa gcaactagtt ttgttaaaca aactatttca
    13921 tctgaagata ctttgtcgaa tgcatattgg acaatgaaaa tttttcaaga tttattggct
    13981 tttagattgc aacaaaataa tcctgaagca ttagcggatg cggatattaa aagattgaac
    14041 tatgtcaaag agcattatac tgacaaagat gaagctaatt tcctttatta tcaagcatta
    14101 ttaaatctaa atactcaatt tgcaaaatcg gcttatggcg atgaaataca atatttattg
    14161 gccaatttct ttaatcaaaa tttagataat caatatttaa aaaataaaat tgaaaataaa
    14221 gatgttttga cgactcaaaa tattatgaag atttgtaatg attgtataaa aaattatcca
    14281 aattcatcag gagcaactaa ttgtttggct ttaattaaat tattacaaca aaaaaatatc
    14341 catattacaa ccgaagaagt tttgttgcct aatcaacaaa gtttagcaaa agttacttat
    14401 aaaaacatca ataaagttta tctaaaatgg attaaagttg attatgataa aagagataaa
    14461 ttatttgaac taaaaaataa agaaacacaa gatgatatca tcaagcgatt aaatgaatta
    14521 aaagttacaa agtcttggag tcaaaattta tcgcaaaaaa tagattatac agaacatacg
    14581 tctgaaataa ttttgccaag tgttgaacac ggattttatg ttttaatggc atcaacgaat
    14641 tcaaatttta atcttaaaaa tgatggaaat gcagttgtga ttaatcaagt atttgtatct
    14701 aatattggat atattacgaa ggcaaattca aatgaaaatt ttgaaattta tgttttagat
    14761 agaaaaacaa gtaagccatt gcaaaatgca acagttacac tttttaaacg tgaatataat
    14821 tatcaaagtg ggaaatatat aaaaattaaa gtaaccaaac tttcaacgga ttccaatgga
    14881 tatgcacaat ataaaaacac tttaaaacca gcagaaacct attcttctaa ttatttttta
    14941 gttgaagtaa attataaaga tgattactta gtaggagatg aaacgtacta tcaaagtaat
    15001 tataaaactt atgaagaaac acctcaaaaa acagtacatt tctttaccga tagagccatt
    15061 tatcgaccat cacaaattat ttattttaaa ggtattctga cggaatatta cagagaggaa
    15121 aattatattt tgcctaataa acctgttacc gttgttttca gagatgctaa tggtcaagaa
    15181 atttctaagc aagaattgac aacaaatgaa tttggttctt ttacaggaat atttactgca
    15241 ccatcaaatg gtttgttagg tcgcatgtcg ctggagacaa gttatggttc tgttggaatt
    15301 caagtggagg aatacaaaag acctagattt gaagtgcttt tggatactat aaaaacagct
    15361 tataacttaa atgataccat aaaagttaca ggtaaagcac aaagttattc tggagcaaat
    15421 gttgatggtg caaaagtaaa atatacagta actcgagtgc caaattttcc agtatggttt
    15481 ggttggcgat ggttttttcc tatgccatca acctctgata aaattattgc aacaggagca
    15541 actacaactg atgaacaagg aaaatatgaa tttaattttg aagcaattcc agatttaagt
    15601 ttagataaaa aatacaaacc ttatttcaat tataaagtaa atgttgaagt aacggacatt
    15661 aatggagaaa cccaaacaaa tcacacagaa gtgcgtgtcg gttatttagc aattgatgca
    15721 tcggtaaatg tgccgagtta tatagataca aagcaagcga atgaaatttc agtgaacact
    15781 aacaacttaa acggacaacc agaacctact gatgttgaaa taaaaattta tagcttaaag
    15841 caacccaaaa atcctttaaa atcaaagctt tggacaacag ttgatgaaca attaattgca
    15901 aaaactgaat ttgaaaaact tttcccttat gatgtttata acaatgagaa tgatgcaaga
    15961 aattgggaaa aaggaagctt agtaaaaacg tatagtttca atactaaaga ccaaaaagct
    16021 attttattta aagctaatga atgggaagca ggaacttatt tggtagagtt ttcttgtaag
    16081 gataaaaatg acaatttgat tgcgttttca aatacattta cggctggaac ttatgcagat
    16141 aaattacctc aacctcctga atttttgagt ttgcaaatag ataaaagtag tttgcaacca
    16201 ggtgatactt taaactataa aattagtagt agtttaaaaa atgtctatgt cgttttagaa
    16261 gtcaaacatc aaaataaatg gattgataaa aagattattt taattgaaaa caatacggag
    16321 ttttttaaat tacccatcac tgaagaatta agaggcggat tgcaaattag atgttatgct
    16381 ttattagaaa atagagcctt tgcacaatat ggatttgttg cagtacctta tccaagtaaa
    16441 gatttaaaaa tggaatggct gacatttcga gataaattat tgccaggaca aaaggaaact
    16501 tggaagttaa aaatttcagg aaacgataag gataaagttg ttgctgaatt agtaacaaca
    16561 ctatatgatg catctctaga tgcattcgta aagcacaatt ttgatttcta tttacgtgga
    16621 aaaacgatta caaataattt gagtgcgttt caatcaaacg atggttacgg cgtttcaagt
    16681 agttctattt atacatcaaa aaaatggaat gaatatggca aaaccattta tcaaggatat
    16741 gatgctttaa atttatttgg gttgcaaatt gcttatccaa gatattattt ataccgaaaa
    16801 ggtggaggcg gaatcatgat gaatgcaaaa tcaggagtaa tgacagagtc agttacgtta
    16861 gaagctccaa gcctttctcc aaaagagatg gataaaaatg tatcagacga tattgcaatc
    16921 gaagaaaatt ataaagataa agtagaaact actgaaccta agccaacaga aaaagtagat
    16981 gtaaatccaa gaacaaattt gaatgaaacc gcatttttta taccgcaaat gcaaacagat
    17041 aacaatggaa atatcttgtt agaatttacg atgccagaag cattaaccaa atggaactta
    17101 ttaggttttg cacatactaa agatttatat acaactcaat tctcagaaag tattattacc
    17161 caaaaagaat taatggtcgt gccaaatgct ccaaggtttt taagagaagg agatacatta
    17221 tatttttcat ctaaaattac caatttatcg gaaaaagatt taaacggtaa tgtatcatta
    17281 gtaattaaag atgctttgac agagcgagaa gtaaatactg agttctcgaa taaaaatcat
    17341 acacaagcat ttgctgtaaa acaaggacaa aatacagctg ttttttggaa gattattgtg
    17401 ccaaaaggct attctgcggt tacttatgaa gtattggcaa aagcggagaa tttttctgat
    17461 ggcgaaaaaa atgtattgcc agtgttgagt aacagaataa tagtaacaga atcgttgcca
    17521 ttatggattt caggcaaaga aaagaaaaaa gttactttca ataaattgtt caataacaaa
    17581 tcaaaaacat tagaacatca tgcattgact ttagagatgt cgagtaatcc tgcgtggtat
    17641 gctgtacaag cactaccata tataatggaa tatccttatg aatgtgcaga acaattattc
    17701 tccagatact atgctaatag tttagcatca catattgcca attcaaatcc aagaataaaa
    17761 agtgtgtttg aagaatggaa aagtacggat agcaaagcat tgttgagtaa tttggaaaaa
    17821 aatcaagaat taaaaaatgt gttgctggaa gagtcacctt gggttcgtga agcacaagat
    17881 gaaacagaac agaaaaagcg aattggctta ttattcgact taacaaaaat ggcttatgaa
    17941 caacaatcag cattagaaaa attgaccaaa ttacaaagtc caaatggtgg gtttatgtgg
    18001 tttgctggta tgcctgaaaa tagatttatt acacaatata ttgttcaagg aatcggacat
    18061 ttgaatcaac taaatgtgat agatatttca aaagataaaa atctttcaca agttgtagaa
    18121 aaagcaatta cttatttaga tggaaaaatg caagaagatt ttgaaagtat caagaaatac
    18181 gataaagact atctaaaaac aaatcattta ggttatgatg tcattcaata tttatatatg
    18241 cgttctttct ttaaagagaa caaaattgat aaaaaataca gcgaagcgta taattattat
    18301 ttagaccaag ccaaaaagta ttggaatgaa aataagaact tacaaatgaa agctttgcta
    18361 agtatgattt tgtatagaaa taatgaattt aaattagcta aaacaattat tgaatctttc
    18421 cgtcaaaatg ctatcaattc ggaagaaatg ggcatgtatt ggaaagcatt ggacaatcca
    18481 tcgtggtatt ggtatgaagc accagttgaa acacaagcat taattattga agcatttaat
    18541 gaaattactg atgatgaaaa atctattgat gccatgaaaa tgtggttgct aaaacaaaag
    18601 caaacgacaa attggaaatc aacaaaagca actgctgaag cttgttatgc attattatta
    18661 agcgggacgg attgggtaag taatgataaa caagtggaag taaaaatcaa taataaaatt
    18721 ttagatacca agcagttaga tattgaagca ggaacaggct attttaaaac caaatggttt
    18781 ggtgaggaaa ttaaacctga aatggcaaaa attgaattaa aaaaagtgga taaaggtcca
    18841 gcttatggtg cgatgtattg gcaatatttt gaagatttag ataagattac aggagcaaat
    18901 accaatttgc aattattaaa aaaatatttc ataaaagaaa caaccaataa aggcacagtt
    18961 ttaaaagaaa ttacagccaa tacgccaatc aaagtaggtg atttaattac ggtaagaatt
    19021 gaactgaaaa ctgaccgaaa tttggaatat gtacacttaa aagacctaag agccgcagga
    19081 acagaaccta caaatgtcat ttcaagatac aaatggcaag atggatttgg ctactacgaa
    19141 acaacaaaag acgtggcaac gcacttcttt atagattggt tggctaaagg aaattacgtg
    19201 tttgaatata cattaagagc atctttagca ggaaatttct cttcaggaat tacacaaatt
    19261 gaatgtatgt atgcaccaga atttaaggca cacagtcaag gcgaaagaca aataatagaa
    19321 gagtaacgaa gttacgcttt cattgttttt tggaaaaaat cattggcgat ttgcattatc
    19381 cattccgaag cgaaagtgaa tttatctttt ccccaatttc cgaattttat atctggagag
    19441 gtgaaaatat aataatcatt atttttgaca ccttttagga ttgattttgc agcattttca
    19501 ggacttaccg catgtcgctg gaattgcttt tttaaggttt taaatttctc atcggtaatt
    19561 tctaaacctg aaattcttat ggtacttacc aatccagtat ctactgcacc aggacaaact
    19621 aaatgtactt ttatcttatg tcgttttaaa tcgtgtctaa gtacatcaga caaaccacgt
    19681 aatccatatt tgctggcaca ataagcacca tgccaaggta aaccaaacaa ggctgctgct
    19741 gatgccacat ttactaaatg accaccattt ttttcagaaa tcatttttgg caaaaaagtt
    19801 tctataatat ggataggtcc cataagatta acatctataa cttgcttcca ttcctcatgt
    19861 tgcattttat ctaccgcacc ccaaattgca atacctgcaa tattcattac aatatctaaa
    19921 ttgccaactt tttggattac ttgttctcca aattgcttga ctttttcaaa gtttgtaata
    19981 tccaaagctt ctaaataagc aatatttcca tcattagctt ttatttcatt tgcagttgat
    20041 tccaattgct cttgctggat gtctgtaagg ataagcactg cacccaattt gctcaattgt
    20101 aaagcaactg ctttcccaat accacttgca gcaccagtaa tcaaacattt tttgttttgg
    20161 aagaaattat tcatattttt ctattttctt tttagaatca taaactcaga atcaaactca
    20221 ttcttttcat tttttggaat atattctatt tctgatattt gccatttact ttcatcaaaa
    20281 ctaaaaaaag catcgccttc tagctccgta tctactttag taagataaat agtatctgta
    20341 atggacaatg cttgcctaca gatttctcca ccaccaatga taaataattc tttctcattt
    20401 tgtgctttag caaagtctat ggcttgttct atattattgc aaatcaccac accttcaata
    20461 gtcaaatctt tattcctgct gataataata ttggttctgt taggtagtgg cttcccaata
    20521 gaatcatacg tttttctacc cattacaata caatgtgaag aggtcagttt tttaaatctt
    20581 tttaaatcgt ctgataaatg ccaaggcatc tggttatcac gaccgataac accattattt
    20641 gaaactgcta taatgataga gatttgcata tattattttg tttttaaaga tacaattaaa
    20701 ccttagaaat tcagaaaaat taaattaatg tggatagaat gtcaattgaa agttaaaata
    20761 ttacttataa ttaattgatt ttagctaact ttataaaaaa aagcaacaca atgaagtttt
    20821 taaaagtttt agccatatta ttttttgcgg taggtatttt taacaaatgt aaaaacaata
    20881 aagaagattc agtaaaaacg gaggttttaa cggatgaagc atttcaaaca aaacttcaag
    20941 aacaattgat attagttgaa gatggtgaca caatcgaaat tccagaagga aaatattcat
    21001 ttacaaaaac actatcctta gaaggcaaaa aaaatataac tataaaaggt gcaggaatcg
    21061 ataaaactat tttgtcattc attaaccaaa cagaaggtgc agaaggtata aatattacga
    21121 attgtgatgg tattactatt gaattattta cggttcaaga tgccaaagga gataatataa
    21181 aattgaaaga ttgtaataat gtaattatta gaaatatgaa ttctacttgg acaactggag
    21241 cggattcaac caatggaagt tatggctatt atcctgtttt gtgtaaaaat gttttggtag
    21301 aaaattgtga agtttcttat tgctcagatg cgggtgttta tgttggacaa tcaaccaatg
    21361 taattgtaag aaattgctat gcacaccata atgttgctgg aatagaaatt gaaaattgca
    21421 tcaattctga cgtatatgat aatcttgcag aaaataatgc aggtggaatt ttgatttttg
    21481 atttgccgaa attatttcaa gcaaatggta gaaatgcccg tatttttaat aataaatgcc
    21541 tcaataataa ttttaaaaac tttgccaaac caggggcaat tgttggcagt attccatcag
    21601 gaactggtat gattattatg gcaacagata gtgcagatat ttataataat attttccaag
    21661 ataataaaac ggtgggagtt tcgattgtaa gttatcatat tacaggtaaa cctgtaaatg
    21721 attctgctta tggagcttac agttatgcta tttctgttca tgacaaccaa ttttcaagaa
    21781 aggcagtatt aattccagat ttgaaaaatg atttaggtaa attattattg gcaactgcta
    21841 aatctcctgt agatattata tatgatggag cttctgacaa aagtgtacgt ggtaaaaatg
    21901 gtagtttgcc agaggataaa agaatttgta ttagaaataa tggcgatatt aagtttatga
    21961 atttgaatgc atggaaagcg gaaggtattg ttgatttagt gaaatactat gatatggaca
    22021 ttactaaatt tgattgcgaa atacaaggta tattaggcaa taatactcca acaaaataga
    22081 tatgaataag acaaaattta tatttttttt aattgtatgt ttattgattt tcgctcaatg
    22141 taaaacaaaa tcaaaaacag taaatcttta ccaagacgga acgccgtttc aaaaactatc
    22201 ggaatataat ttttttaaag gaaagataag cgatttgatt ccaaatgaaa gcgttttgcc
    22261 ttatgatgtt gccacgccac tttttacaga ttatgctgaa aaagcaagat ttgtctggtt
    22321 gccagaagga actagtgcaa attataataa agatgaagca ttagattttc cagaaggcac
    22381 catcttgata aaaaacttct attattggaa tgattttaga gaccattcta aaggaaaaag
    22441 gattatagaa acaagacttt tagtaaaaga aaaaaaaggt tggaatacta ataattattt
    22501 gtggaatgac gcacaaacag atgccacact ggatgttgtt ggcaaaacta ttcctataaa
    22561 atggataaat gaaaaaggtg aacaaatggg tacaaattat tcagttccaa acaaaaatca
    22621 atgtaaaaac tgtcataatt ttaacaatac ttttatgcca atcggaccta aagtgcgtaa
    22681 tctaaatcac gattttactt atcaagaggg tactaaaaat caaatggaaa aatggacgga
    22741 agtaggttac ttaaaaggat ttgttgccgc agataatatt cataataaac taccaaaatg
    22801 gaatgaccaa aaaagtggca cattggaaca aagagctaaa gcatatttag aatctaattg
    22861 tgcacactgc cacagaccag agggaaatgc caacaccagc ggtttgtttt tgctgacaac
    22921 agaccataat ccagaaagtt ggggcattat gaaaagtcca gttgctgcag gaaaaggaag
    22981 cggtaataga ttgtttgata tagttcctgg aaaatctgat gaatctattt taactttcag
    23041 aatggagagt ttagatgtag atgcaatgat gcctgagtta ggaagaacaa tggtacataa
    23101 agaaggtgtt cagttgatta aagattggat agcgagtatg cctcagtaac aagatttata
    23161 atactaacta aatattctgt tttctatctt tgtaatatga tacgtacaaa tcgtttaagc
    23221 agtttgcttt ttatttttat aatttccttt gtttttagtg cttgcaaata tagtgataaa
    23281 ccaaaaacat ttaataatgg tgcggtttca ttagagtatc cttcttattt gaaaagtagt
    23341 aaagatattt atccagtgga gaatttatta attggcgtaa aaaatgatta tcggaatgtc
    23401 ttttttattt tgttagatta tggacaaaag ccaggggaac aaggttttga gttgatgtat
    23461 gattcattat cgactcaatt aaaacgaggc atcagagaac catttgtaga aaaagattcc
    23521 agctttaata ttaatggatt taaagttcgt gaaatgcaag tttctggtat tttagcatct
    23581 gtaggccaag aaaaaagaat gttgtttact tttgatttat ttgaagataa aaacggacat
    23641 ttgtaccaaa ctgcgggttg gtgtttcaga catcgaagag atttatggga aaaggatttg
    23701 caacagattg cctattcttt aaaaagtttg aaataactaa aattcttctc taataacttg
    23761 atgaatacct tctgtggcat tcaatttttc catcaattta tttaattgat tgttgtcttt
    23821 gacatagatt ttaataatgc cttcaaacat gccatcaatc gattctaaag aaatagattg
    23881 catatttagt ttcatttctt gagaaataat atgcgtcaat ttgttcatta ttccaatatg
    23941 atctaatcca tttaatttta tagttgtcaa gaaagaaggt gctgcttttg aagtccaact
    24001 tgttttgata attcggtagc catatcttga tgctaaattg atgatgttgt tgcaacctgt
    24061 cctatgaatt tttataacac catttattga aataaaaccc ataatatcat cgccaggaat
    24121 tgggctgcaa cattttgcca attcataagg gaattgactt gaactctcgc caaaaataac
    24181 caattcagaa ttgtttgaaa aattggactt tggagcttca aagacttctt cttttttttc
    24241 tttttctatt ttaggaattt ctagattgcc accaacaact ttaagctgtt ttatgttatt
    24301 cagtgtgata gcttctctgc caatatcata aaataaatcc aaactacttg gtgatttata
    24361 aaactggcaa agcacattta tattctcatt cgttaatgaa cattttaaat gagaaagctt
    24421 tcgttccact atttcttttc cgtgttcagc aatcttcttt ttttcttctt tgagggagtt
    24481 tttaatcttt tgtctagctc ttgaagttgt tacgatatta atccaatctt cggtaggttt
    24541 ttgttttttt gaagtaataa tttcaacttg gtcgccatta gataatttaa aactaatagg
    24601 cactaatttg ttattgactt ttgctccaat tgtttggtcg ccgacattgg tatgtatagc
    24661 ataggcaaaa tcaagtgtag ttgcaccaac aggaagaaca cgcatttctc cttttggtgt
    24721 aaaaacataa atttccttat tgtataattc taacttgaaa tcattgataa attcaacagg
    24781 attggtttct gggtttttca aaaactctcg cacttgcatt aaccacgttt caatggcttc
    24841 ttcagcagta tgcacttgct tttgtgcggt attattttct ttatatttat aatgtgctgc
    24901 taaacctttt tcagcaattt cgtccattct ttcagatcga atttgcactt caacaaagtt
    24961 tccaccaggt cccatcactg tagtatgcaa agattcataa ccatttgctt ttgggtgcga
    25021 caaccaatct cgtaaacgat taggctgtgg tgtatattta tcagtaacaa tagaataagc
    25081 ttgccaacaa tctgctttct ctttttcagg cggagcatca atgataattc taatcgcaaa
    25141 cttatcatat acatcatcaa aacttacgcc ttttttcttc attttatgtg caatagaaga
    25201 gatggatttt ggtcttccga aaactcgagc tttaatacct atcctatcca tttcatcttg
    25261 taaaggcttt acgaagtcgt tgataaatcg agtgcgttct tttttggttt catctaataa
    25321 tctagcaatt tctttgtatt tctctggctc caaatatttc attgccaaat cctccatctc
    25381 acttttgata gaatataaac caaaacgatg tgctaaaggt gcatacagat ataatgtttc
    25441 agaagaaatt tttaattgat tctttttaga aacatcattt aaggtacgca tattgtgcaa
    25501 cctatcggct aattttataa gaataacacg gatatcatct gaaagcgtca gtaaaatttt
    25561 tctataattt tctgcttgta tagattctgt ctggtctgat attttactga tttttgtaag
    25621 tccatcaata attttagcaa tgacaggccc aaattctttt ttaatatcat caattgttac
    25681 atcggtatct tcaacagtat catgcaataa agcacagata acagaattaa cgcccaaacc
    25741 aatatctcca caagtgattt tggcaactgc aataggatga tgtatataag gttctccgct
    25801 ttttcttctt tgatgtctat gtgcttcaac agccatttca aaagcacgtc gaactcgctt
    25861 atcgtctccg gcttgcattc ttggacgcaa cagtcggaat aactgcttgt aatccttcag
    25921 gatttccttt ctttctaact cttctgcttc cgaacttaac tccattactt gtattgtata
    25981 ataaaatcct aaattagaat taaatttaca atataagaaa taaagtttag gttaaactcg
    26041 aattattttt tctgctttaa ttttgcgttc atgccttcaa tgatatcttt agtgatatca
    26101 aatctttcat caacatataa taagtttgga ttagactttg tataaggtaa tatataagta
    26161 tatctattgt ctgcattata ctctttcaag taaacagtta tcgattccat taattcaaga
    26221 ttgaattttt ctgtcatttt catcaaagca tctgtagaat tttgtacttt ttcttgagcc
    26281 ttttgttgtt ctacagccaa tttttgttga gcaatatagt attcttcttc agtcattgtt
    26341 tcggctttct tttgatagcc ttctatcatt gattgaattt tcttctcatc tcgagccatt
    26401 tcactttcga tagcgttttg tttagtctct atttcatgtt ttttttgttt gtaataggtg
    26461 taattatttt gaaatgaatc aatatcaaaa tatgcaatta cttgaccttg taccgcttga
    26521 gtctttccat ctaaagattt tactgtaact ggagctgcac ttttcttgcc gttgaaatgt
    26581 aaatagaata aaatgcctac taaaatgaat aaaacagcat ttaagattaa agataaattt
    26641 ttcatggtgc aaagataact tttttaaaag aataattaga tagttcgtaa atgcttaaat
    26701 tagaatgatt attgtctttt aattaggtat tttatttcta aatatattaa aagcgaattg
    26761 aaaaattagt ttatttttga aatcaaaaat tagggaatgt gccttattaa gattgataaa
    26821 agcaataatg tcattagtat aaatagtgtt tttaatattt atggtactta tgccaacgag
    26881 cattatgcca attgcattaa aaatgagatt gaaaccatgt ggaatgctcc aaaaggaatt
    26941 gtacagcttt ataatcagtc atttaatgtt tgttttaagg tagatgttgt tatttatgat
    27001 ttaatttcgc ccacatcaat tttagaaaat gataatttcc ttaataaata tatcagaatt
    27061 gaagaagtaa gtaaaatgca ggtgtctttt gttgatggga ttaattcttc aacgggctat
    27121 tttactatca ataatttaga acaaggaaca actgttgcac atgaatttgg acacatgctt
    27181 ggtttgaaac atcctgaaaa tttagactat agaggcaaag gtcgcccaca tattatgtat
    27241 ccaagaggca cactggtaga tgccgaattt caatatgatg caaatgctat tgcagggcaa
    27301 cctggtggaa cagtgaaccc aacagtaaga gttgttaccc aagaaaatat tgatgaccta
    27361 aaactgggca atataataga acaaggacaa gcacatattg gcaaaataag caccaattat
    27421 cacgatattt ttattaaatc agcacctaca caatcagcgt aaaattgctt atttataaag
    27481 ttcttttgct tgtgttttct taggcaatta atcaaaatta ttttcaaaca aaggctttaa
    27541 tttctagttc agaatttagt attagtatta aaagatttta taacttagct atccgaattc
    27601 cacataaaat tgaattggat ttttgaacga tgacaccttt gaaattacat acaggcagta
    27661 ttatagatat attctcggca aataccgaaa caaaattagc attaccttat gtagaagaag
    27721 gaatatctgc aggctttcca tctccagctt tagactttgt cgatttaacg attgatttaa
    27781 atcgacattt aataaaaaat ccttcagcaa cattttatgg taaggtaaaa ggagattctc
    27841 ttaaaaatgc aggcattaat aatggagatt tactcgtcat tgaccgaagt ttagacccaa
    27901 ttaatggaaa aattgcagtt tgttttattg atggagagtt tacagcaaaa cgtattcaaa
    27961 ttttaaaaaa tgaaatttgg ttagttccag aaaacgaaaa ttacccttcg atacaaataa
    28021 cagaggaaaa taattttatt atttggggta ttgtaacaca tgccataaaa aacctttaga
    28081 tgtttgcttt aattgattgt aataattttt acgcttcctg cgaacgggta tttcgtcctg
    28141 acctaaatgg taaacctgtt gttgttttgt cgaataatga cggctgtgtc attgcacgca
    28201 gtaatgaggc aaaggcattg ggtattccaa tgggagctcc cgcatttgaa tatgaacaat
    28261 tatttcaaaa agaagcagtt cacgtatttt ctacaaactt tgcattgtat ggagatatga
    28321 gcaatcgtgt gatgagcata ctttctgact acgctccaga gattgaaatt tatagtatag
    28381 atgaggcatt tttaaaaatg gatgggtttg atttgtttaa tttacaaact tatggcgaag
    28441 agatgcgtca taaagtaaca aaatgtacag gaattccaat aagtgtgggc attgctccaa
    28501 caaaagcctt agcaaaagta gcaaatcgta ttgctaaaaa atatcccaaa caaaccaatg
    28561 gcgtttatat tattgataat gaggaaaagc gaattaaggc attaaaatgg cttaaagtgg
    28621 aagatgtttg gggcattggg cgacaacacg ccaatcggct aaatgcaatg ggtgttaaaa
    28681 cagcttggga ttttacatta ttagatgatg gaattgtgcg aaaacaaatg tctgtagttg
    28741 gattgcgttt aaaacacgat ttgcaaggta ttccaacgct tgatttggaa gccattcaaa
    28801 tcaaaaagaa tattgcaacg acacgaacct ttgaaacaca ttataccgaa tatggacagt
    28861 tagcagagcg tatcacaacg tttgcatcat cttgtgctga gaaattacgc aaacaaaact
    28921 cttgttgtaa ttccttgatg gtgtttatac aaacaaatat gcatcggaat gatttggcac
    28981 aatataaccg cagtatggta ttaaaattac cttttccaac aaactcaagt attgaattag
    29041 caaaatttgc aaagcaagca ttgcaatata tttttaggca aggatatcat tataaaaggg
    29101 caggcgttgt ggtgcaagat ttttctcagg gaaatcagtt acagcaaact ctttttgaac
    29161 aacgagatga acgacatatt cgattgatgc aagcaattga ccaattgaat aatagacttg
    29221 ggcaacaaaa aatacgcttg gcatcgcaag acattaaaag agtttggaaa atgaaacaag
    29281 agaaacgctc cccacaatat accactaaac taaatgatat tattacaatt aaggtatagc
    29341 attatattac agcatttact gcataaagtc ttatttataa agccctttgg cttgcatttc
    29401 atttaatgat tgctcaaaat tattttcaac taaaggttgg aattcctact atttttggta
    29461 ctcaaaagct taacaaaata gcgtagataa aagctacaaa accacttata gaaaaaaact
    29521 taatcgtgtc gttgaatgat atgctgagct tgtcgaagca caatctcgct taaagcgaga
    29581 caaaaaaaca gagccaacgc tcagggtttt acatttcagt gcttttttct attcgtcata
    29641 atgttgggca atgtgttaaa attggaaaaa aatttgtttc gattgacaaa atttataact
    29701 ttgcaataag tgtcaactaa tgattaatgt tatgaaagaa attgtttccc aaaaaatacg
    29761 acaaggtagg gttcttaatg cgatgtcatt acaagaactt gctgacttaa taggtgtttc
    29821 aaaacagatg atttcaaaat atgaaaatgg aacttcaatg ccatcaagta aaatattaat
    29881 ccagttggca aagtcactga acgtaaagtt agattatttt tttgcacctt catctgtaga
    29941 attgggtaat gttaatttcc gtaaaagaag ttctttttct gtaaaaaaaa ttaatgcttt
    30001 aaaggaagat attaaaattc aattgtcaaa ttatttagaa attgaaaata ttttaaagat
    30061 tgataactca tttgagaatc ctttaaaaaa gaaaaaagtt aactcattag aagacatcga
    30121 aaaagtagtg agagaattaa gaaatgattg ggagattggt tttgacccaa tacataatat
    30181 tattcaactt ttagaagata acgaaataaa ggtaattgag attgatgaac cacaaaataa
    30241 ttttgatggt cttgctgcta ttgttaatga taagtatcca gtaatcgttg taaataaaaa
    30301 ctttattgtt gaaagaaaac gttttactct tttacacgaa ttaggacatc ttctattaga
    30361 tttaccagag tgtgacatga aatttgaaga gcaaatttgt aataggtttg catcagaatt
    30421 tttatttcct aaatcaatgg tccttaaaga attcggtgaa ggtagaggaa gtatatcttt
    30481 taaagaatta atagaagttc aacgcaaata cggaataagt atcaaagcta taatttatag
    30541 gttaaaagac tgtggtatat taagtgagaa tagggtagct gaattttata gaaaaattaa
    30601 tttcaatcca agattaaagg acgaggttga tgctcaaaga tttcaaactc ctgaattttc
    30661 aaatagattt gaacaattag tttatcgagc atactcacaa gagttaatat catctagtaa
    30721 agctgcttca ttattaaaca ttaatatcaa tgaggtttta gaaaattcaa caatgtaaat
    30781 ggaatgagga tagtaataaa tgatgcaaat atcttaattg acctatttca tttagattta
    30841 gttgaagtgt ttttcagttt acaaggtttt gaagtgaaaa caactgattt tgtctttgaa
    30901 gaattatatg atgagcaaaa aaatattatc aagcaattta tacaaaatca atcattgatt
    30961 ttaatagagt catctgagga tgatttaatt aatatttttg aaattttctc ttctactaaa
    31021 ggattaagtg ttgaagattg ttcagtatgg tatcacgcaa agaaaaataa aggaatactt
    31081 ttaacaggcg atggcaattt aagaaagcaa tctttagctg atggtgtgga agttagaggg
    31141 atactgtaca tttttgatca attattaatt tctggcttaa tatcatttga gtttgcaatt
    31201 gaaaagttga accaattata ttcgataaat gaacgtttgc ctattgatgc aaaaaagcag
    31261 agattggact gttggtctag gtcagaacat atcgcctaac ttcactcctt ataaaaaatc
    31321 atttaattga aatataacca tttacccgat tcaaacaatc ggttctaagc tacaatacaa
    31381 gcactgaaat ttagactaga aaaattggtt ttgaaaaata tagagcattg tcctaatcag
    31441 tattgccttt tagtaccatt ggctacttca ttgccctttg ttttccctta atttttgtgt
    31501 ttaaaattct ccacttgtcc catcacttga tcaaaaatct catcattgta ttgaggtgga
    31561 taaccgctat tatgtaaaca gaagaatatt ttttgattga ggtctgccct tacattgcta
    31621 ttattgagcc aatcggtaaa tgatgactgt acatcaatca actccttaat tttctgagct
    31681 aagaccttac atttatcatt tataattaag cctcccactt ttttatcttc gccatactca
    31741 aagttgtact tgtcacgcag atgcatcaga atatcataaa atgctttttc ctcaaaagtc
    31801 aatcccaact ttctaaagct gtctttgtct tcccctaatt ttgaaagtat atccaaagcc
    31861 tgttgcgtag catttctgat aatggtgtcc acggcttctg cttgtgtttc actagcctct
    31921 gaagaagata aactcgctct tcggttgtga tattcttcca atgttttatt cagcaattct
    31981 tcatattttt ctgctgctat tttgttggtg tctttatatt ctttgattga tttacgaagc
    32041 atcttaatta agacttccaa tttggtggca ggtaatttta tgctatccaa ctgttctaca
    32101 aactcggggc taaatatatt ttcttctaca tcggtctgta aaatgctcac cacagcattg
    32161 cattttaagg cttctgctac cattttttca actgcacggt tcatactttc tgtatcgtgt
    32221 ttttcgcccg aagtttttct gatataagat gccactgcca taaaacactg cgataaggaa
    32281 agtcgctcat tgtcaagtac attagatggc tgacaaatgt cataagctgt tctcaaacgc
    32341 tttacgtgag ctaagaaaaa tgtttttacg ccaaccgctt ttggtttttc tttttcatcg
    32401 ctcaagttta gtgtctccgt taaggtaatg atatagtcgg ctgctgccga caagcactct
    32461 aatctttcta atggagtggc tttggggtca gtaaacggag tgatgtcaaa atcaacaaat
    32521 acatctttga gtatttgcaa ttcaataatt aatgcctcta atgcttgttt tacatcatct
    32581 tcgctcggtc caaatgtttc accgccaaat tttcgcatcg cctccatcat attgtctcga
    32641 atgccgatat agtcaataat atatccgtac tctttgccct catattttct attgacacga
    32701 ctgatggttt gaataagcgt ttgtttttgc agtggtttgt cgttgtatag ataggttaga
    32761 gaaggcacat caaaacccgt aatccacata tcgacaacaa taacaatccg gaaattagat
    32821 tgctctgttt taaaggcatc atctaacttt ttatggcgtg ctttatcgcc caaatagtcg
    32881 tacatatctt ctgggtcatt ggctccacga gttgccacca ttgcaatagt tggcattggc
    32941 gagagttctc ttaactcctc agcagtgagt ttactatcgt ccggtgattt tttctcttca
    33001 aaccattcgg gtttatgtgt tttgaatttt tggagtaatc ggtaggctat tttgcgtttg
    33061 ctacatacaa tcattgcttt ttgtaccaca tcagctttgt tttcgcaagc attggaatag
    33121 tgtgctatta tgtctttggc aagtctatcc aaacgctcct catcacccaa aatgacttcc
    33181 atagcactca ttgccgtttt gctggcagct atatcatctt cggttgctcc ctcatcagca
    33241 cattttttgt aaaagtcgtc tatctgtttt actttctcac tgtctaaggt aacattggca
    33301 atgcgaggaa tgtattttag ctccaccgtg atttcgtctt ccacagattg ttgcatggtg
    33361 tagctgtcca ccacatcgcc aaatacttga atggtttcat caatgggcgt acccgtaaaa
    33421 ccaacaaatg tagcattggg aaaagcagca cgcaaatgct cggcataagg cttggtaata
    33481 aatgcgccta agccttctac ttctaggatt tctccatttt cgttttgagc ttttagactt
    33541 ttttggtctt ttatttttaa ctgcttattc aattttactt gcgtacggtg tgcctcgtct
    33601 gaaaaacaga taatgttttt tcgggtattg agttcgccta tctcttcgca gaatttttga
    33661 atggtacaaa taaaaaatcc acctgattcc ctaatggata attctgtttt aaggtctttt
    33721 ctgtcggcaa tgattttggc tgctcccaag gataaaaatt cctctgaacg aaggaataat
    33781 ttgcctgctt gagtttgcag gtcgtctcta tctacaatca tcacaatggt tggcgaaccc
    33841 aactccgtgc aacgcaagga gagctgacgt gccaaaaaca acatggtata ggttttgcca
    33901 caaccggttg caccaaaata agttccgcct tttctgctat tggcttcgtg ggctgtgagt
    33961 acgctttctc tcagcatacg ggtagcaaaa aattgtggat atcgacagat tatctcttct
    34021 tctttgtcgt gatttttgtc cgggaaatac acataatccc gaagaatttc taaaaaacga
    34081 ttgggctcat acacacctgc tacaatggtt ttcacctggt ctattcctct ttgggcagac
    34141 tggtcctcat tatttacttt tttccaagcg tagtaatgat tgtagggtgt gtaggtagtg
    34201 ccaagtttgg tgttgttaat actggcatcg ctgatacaag agagtggaca ataacacaaa
    34261 agatggggta tgtctctttt gtatcgggta tgaatttgct cataagcgtt ggcaatgctg
    34321 gcattaaagt cagttgggtt tttaagctcg aagatgcaaa gcggaatgcc attgacaaag
    34381 agtaatacat cgggaatgcg aacatcgtct tttaatccat agcctacttc aaactgattg
    34441 acacagcggt aaatattgtt gttgttttgt ggctcaaagt ttatcaattc tatatcgaaa
    34501 gaggctccgt cttggctgcg gatataacga aaaccatcac gcagtaaata ataagtattt
    34561 cgcaataact caaactgcgt ttgaccgctt acgtgtcgta gtttattgat gatttcttct
    34621 atatcattag cctgtaaatc gggatagcct tgttgaagat actgggtcaa atcatctaca
    34681 agcagcactt cgcggttgtt gcgggcaatg ctgccaccgt gagtatatgc ccaaccttgc
    34741 tgtgtaagca gttggataag ggcttcttcg taatcgcttt cgtaatattt acctttcatt
    34801 tttatttatg cgtttctttg gtgtgcacta tccgtgcatg gctttttgca ccaatgccgg
    34861 gcagagcgtt ttcatttttt cacgagcctc agcagctatg tttttagcct cctcggcaca
    34921 gtgatagaga ttgacaatgg cttgttgcac ttcgagcggt ggaagtggga ttttagttcg
    34981 acacaattca ttccagtcaa aagtttcacg agcacttccc caagaattga aacgtgagta
    35041 acgatcaaat tcctctcgac ttaacaaaag aaatataaat tcaggtaata atttttcagc
    35101 atttattgtt ctaaaaacca aatatgaaga cgaaactata aaggtttctt cagtatcatt
    35161 tattgctaag gatattttac ctccatttcg tgatgttaca gttacataac tgaaatcttt
    35221 tggctttaat aatttatatg gagacaatga tacatcttgc atatctgcct ttgtaggaat
    35281 aagttttttt aagatactta ttccttttac atcatcaagt gtaaatttca aatcattatt
    35341 tctttcgtca ctaagttcta tatactcccc caattgcact tcgggatact tttttttgca
    35401 atctacaata taggcttggc aagcctgggt gagtggttgt attaaggctt cgttttgctc
    35461 tgccaatgct tttagtccgt tataagtatc tactaactct tgttgg
//
LOCUS       DAOQCW010000009        35152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_426_length_35152_cov_5.485284, whole genome shotgun sequence.
ACCESSION   DAOQCW010000009 DAOQCW010000000
VERSION     DAOQCW010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 35152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35152
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_426_length_35152_cov_5.485284"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="PLS16_01670"
     CDS             complement(<1..1040)
                     /locus_tag="PLS16_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNJ59814.1"
                     /translation="MRLQKSFLLPFLLLMAIVAAGAFNPGQSIQGSTASTIDTGNTAW
                     MIVATALVMIMTPGLGLFYGGLANKKNIISTVLQSFISLGIISVIWVAFGFSLCFGED
                     VGGYGIIGNPFTYFMFKGVTLTPQEGSTIPFILFAMFQLKFAVITPALITGSFAQRVK
                     FSAYLIFIVLFFIFIYAPLCHMTWHADGIFFKMGVLDFAGGTVVHLSAGFAALAGAIF
                     LGPRTESERTHEYSNIPLIVIGTGLLWFGWFGFNAGSALACNTVAANAVATTNTAAAA
                     AMVAWVFLDALLGKKISAIGACVGAVVGLVAITPACGFVNTGESIAIGAISAMVSNFA
                     VHYKNKSSLDDT"
     gene            1326..2048
                     /locus_tag="PLS16_01675"
     CDS             1326..2048
                     /locus_tag="PLS16_01675"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HNJ59815.1"
                     /translation="MEKFKVEVWSDIVCPFCYISKRNYEKALTLFEFSEQIQLEFKSY
                     QLDPDFIQNQDEKYDIKAYLANKYRRSLEEIEDMLTQITSSAKSVGLNYDFNKVIRFN
                     TLQAHRILMKAQDEGLGNKLMEVLFSAYFEQGLDLGNTEVLKSIALKSGLNEPAFEEA
                     ITDEQFAYRVKQDIQEAANLGISGVPFFVFNRKYGVSGAQPVSVFSDTLEKAFHEWKA
                     STESKIENIGNGATCSIDGNCE"
     gene            2457..2825
                     /gene="ssrA"
                     /locus_tag="PLS16_01680"
     tmRNA           2457..2825
                     /gene="ssrA"
                     /locus_tag="PLS16_01680"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            2890..3906
                     /locus_tag="PLS16_01685"
     CDS             2890..3906
                     /locus_tag="PLS16_01685"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HNJ59816.1"
                     /translation="MDLQLSITYNILLFIFSAIVVWVLCNKLSEIVDAIDLQFNLGAA
                     FGGTIMLAIVTNLPEIAIVFNGALNGSIDLAVGNILGGIAIQSTLLILFDFVSRKRET
                     LPFSTLASSETSILQGLFLVAILSMVLIGKQFPESFIVFRTTPPVFFILILWILSILA
                     MKQFQNSNLLKNTTIEEKNMPSKYSKKSALTWLFIISVFVLFFGVILENTSNAIANHY
                     NINGVIFGATVLAFVTSLPEISGGLAFVKSRSYTPIIDDIFGGNAFLPVLFVPTIFLT
                     NNAILPKADRIDLYLTGVAMLITINYLIGMIIKKEQRYFGMGMDSWIALSIYLLSVIG
                     LFFI"
     gene            complement(3925..4695)
                     /gene="trpA"
                     /locus_tag="PLS16_01690"
     CDS             complement(3925..4695)
                     /gene="trpA"
                     /locus_tag="PLS16_01690"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNJ59817.1"
                     /translation="MNRIQKLFKEKKENILSIFITAGYPHLNDLPQILEALQENGVDM
                     VEIGMPFSDPTADGPTIQYSNTIAIQNGMTIQKLFNQLKNIREKIHIPLVLMGYINPA
                     LQYGFENFLKEAAHVGIDGVIIPDLPMYEYENMYQTSFKENGIENIFLVTPQTSNERV
                     LQIDSISDSFIYVVSTHSITGTNIDLEAQQSTYFNRLKQLNLKNPTIIGFGIKDRNTF
                     ELACKNANGAIIGSAFVKHIEQASNLKESIQEFIKGIK"
     gene            complement(4709..5737)
                     /locus_tag="PLS16_01695"
     CDS             complement(4709..5737)
                     /locus_tag="PLS16_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ59818.1"
                     /translation="MIRKLTYIAFIFLLFSCKKDKNSNQFNNYYFLKIDDVALPINIT
                     GNKIAEHAVIYINDGLNSNVNTEKNNVYWQYIENKYKVVYYDQRGSGTAQGNTKPSEM
                     SIEQFAADLDKVIDFTFKIAKVRSVILHGSGIGGAVACFYASDSTRQEKINGLILEAP
                     AYDLKNGLALSKQKVIDLADERIATNNNPGGYWTNLKNYYTEHPVFNSEVFEKHINVL
                     MQNNGLFFNLNNVKQRKTDAPSTPLDVIYKNQLNAYAQIKYNNSYFSEMDMTPLLSKL
                     DLPILLVWGEQDMFLPKDNLALKFKLATGSNVTYNPTKYIQTGHIPHAEDFFNFQVDA
                     LSFINSYK"
     gene            complement(5740..6414)
                     /locus_tag="PLS16_01700"
     CDS             complement(5740..6414)
                     /locus_tag="PLS16_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59819.1"
                     /translation="MNSNYRFIFFIFLSFYFGSLFAQDATLKYKNLLPVEGLKYKKTI
                     ISVAGIADIEFIKPVSINFGMEFGGYIRLFSKAKQIQKKSAYDIKVADRDLFIKPTIG
                     FLYRERYQTGLYFMPSFVYRHTTPKIFYTEISISGGYYYAKLNVPTYEVTPSGEVNKI
                     KVGFSNAIVGGNFNAGLDFSKSGRTPLNLFGGIGLYYNFPNNHNWTRHIIVQAGVAYI
                     IRRNIE"
     gene            complement(6506..7102)
                     /locus_tag="PLS16_01705"
     CDS             complement(6506..7102)
                     /locus_tag="PLS16_01705"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNJ59820.1"
                     /translation="MFTGIIQTIGEIIAIEKKGTNIDFTVKSNFSNELKIDQSIAHNG
                     VCLTVIEQIGDTHKVTAIKETLDKTTLNSWTIGAKVNLEKAMMANAFLDGHLVQGHVD
                     QTAICEKIEDKDGSKYFYFEFVKNPLFTVVDKGSICIDGTSLTIVETAENKFSVAIIP
                     YTLVHTIFHQYTVGTEVNIEFDILGKYIDKILAQRMKH"
     gene            complement(7105..9786)
                     /locus_tag="PLS16_01710"
     CDS             complement(7105..9786)
                     /locus_tag="PLS16_01710"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNJ59821.1"
                     /translation="MQINIKRILLILILFIVSKNIFATHNRAGEIMYRHIAAYTYEFT
                     VVTYTKLSGESEGADRPKLEIFWGDGTKDSIVRISRDVTPFPDVFKNTYRMLHSYTGP
                     ARYIVSMTDPNRIANIINMQNSVNTIFYIEDTVRIFNANDMGYNSSPILYNPPVDAAN
                     VGQRFVHNPNAFDPDGDSLVFSLIPPKQGQSANVSGYLYPNQIQAGINNQFKINPKTG
                     ELIWEYPQVQGIYNIAILVQEYRAGFLIGTVVRDMQIFVESTSNRPPVLTIPENICVV
                     AGDSVNFSISATDADVTQKITLTSNGIVYSLPTQAAIFTTQTPQNPISGSFKWKTDCS
                     HLNKNEYPILFKAEDDFVSPPLVDIKTVFIKVLAPAPQNLTAIGDVSANTVTLNWDNP
                     YSCSTNPKFNKFSIWRKKSCGNPNDSCSTDLEAQGYTFIGYTKNYTYTDNTVQIGEQY
                     SYKIKAEFADLTATNIEVNPFSSLPSDEVCVRLPLSLPIIYNVDVKTTNEINGQIYVE
                     WSRPNADELDTLINQGYYTFTLYRADANSQNYSVIHSKTFTSYSAINDTSYLDTNLNT
                     EFLSYKYKIQFIANAGLDTLGFTNEATSVFLNVTPKYKSIELHWDFNTPWQNDSFVVY
                     RKDPGRSTFDSVTTTTVNNFKDVGLLNDSLYCYKIKSIGTYNIDGLKTPLLNFSQEAC
                     SVPIDTTFPCKPLLNLSNFCLDRHLPENDFVNYLVWNFDPTCDTSTIIKTNIYYKANN
                     YNDYVLLDTMPKNGLLAFTHRLDSVQSLAACYQLETVGKNGNKTMSNMVCSNDCPRYE
                     LPNTFTPNNDGQNDWFTPIIPYRSVEKIDIKIYDRWGILVYQTENPDINWNGTDIKNG
                     KELPTAVYYYTCYLYYKTANGVEKIVEPLSGYIHLFR"
     gene            complement(9869..10912)
                     /locus_tag="PLS16_01715"
     CDS             complement(9869..10912)
                     /locus_tag="PLS16_01715"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNJ59822.1"
                     /translation="MLTDSQKITLIRQEISQSYKDLKQKYPLLKHQNWIGMTFFLLSI
                     SMIVLASVFYIKGHLSVWILIPFNAFWMGILHELEHDLIHWMYFRKNKIIQDIMLFSV
                     WILRPLTINPWLRRELHFHHHKYSGTLHDVEERGVTNGEKWSLKRLFGTPDLIFGGIM
                     RAKILVKDIRNEVRNGNLSKEQALRFKKIKTISMLPFGLIAYSIWYVYLVHLLIVIFT
                     KIFNIDYQTPLFMANQFIWIKPLVAIIIAPNLLRQFCLHFITSNMHYFGDVESGNIMQ
                     QTQVLNIWWTFPLQIFCFFFGWTHAIHHFVVNETFYIRHIARARALKAMQENGVRFND
                     IGTFKRANRYKII"
     gene            complement(10941..11672)
                     /locus_tag="PLS16_01720"
     CDS             complement(10941..11672)
                     /locus_tag="PLS16_01720"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNJ59823.1"
                     /translation="MRQFGLIGYPLSHSFSKGYFAEKFKNENITDAHYDTFPLENIHL
                     IEQLFNEKPNLFGLNVTIPYKQQVIPFLDSLDEAAAKIGAVNTIKFIDGKKIGYNSDV
                     YGFEMSLKPLLEKHHNFALILGTGGASKAVEYVLEQLNINFQYVSRTKSKQTITYQEI
                     DEQLMNKSTVIINTSPIGMYPNIETAPEIPYQFISVQHLLYDLVYNPEVTLFLKKGIE
                     KGAKTKNGLEMLHLQAERSWEIWNS"
     gene            complement(11696..13114)
                     /locus_tag="PLS16_01725"
     CDS             complement(11696..13114)
                     /locus_tag="PLS16_01725"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ59824.1"
                     /translation="MDFFDFDNEEFENADEIRELILQFENAVKQNKPAFFDQDDFESI
                     IDYYEQIAQYDKALRATEQALQIHPNSPSLILRKANLAFQLKDTATAFELIELIQSLD
                     ATDIGSYILKAEILTAQSRYKEALDILKKLENTIESEDLVDICLQISDIYEETSRYDH
                     VFEYLKKCIILNPDEQEALRRINYCIDVTQLYDEGITFFQHMIDMYPYNHHLWYNLGC
                     AFRGIGLTEKAIEAFEYVIAISEDEDYAYIDMIELLIKEKNITKALDVINDLTEVYEP
                     DEEVFLLHGKCFEELGNYKLARYYYKKALHLEPAFGFIYYKIGETYKKEGLWEQAYQS
                     FQKAAELEKEMYDFHIALIESAMEINEIEIAIDACEAAIENYINKEEAYILLAKIFFN
                     LNDFGTALEILHNCKKACKEYTQAIHAEIAVLFMQNKVKECELKLTYLMEEDNLNSDY
                     IYKFHQELEDNTDFQMLIIHYL"
     gene            13167..14447
                     /locus_tag="PLS16_01730"
     CDS             13167..14447
                     /locus_tag="PLS16_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59825.1"
                     /translation="MKNYQIVIHIFALLFLNASFIYAQDSLETTIKHPKLYQIQQKYL
                     NWIDKKDKEKPFALPVIAGNKYDGVQIGAALINLRQPVKNIDFSGVLLYGTKSKKING
                     IADIAYYIEPKNEVLNKIKIATNFKSFSNAISPNKLKYYALKPELEFQLFSKKNSKIT
                     HTINFKSNLIFEQGKVYFSFNHNSDSTLKRFYANDLTYAFDFKNKNYPFNIQVQLEQS
                     KQLLKLGIEANAFIRYQLKDYNTGLHLRFFSGSILYQNSTFRPRSFPNYGYTISGTTG
                     LNDYKYDNFYFGRNVTEGLASRQISANDGFMKLTTPANKNYQEGRTLNYLLAMNIVLD
                     FPIPHIPIKLFADFGYASDKKIHTIQNLPFNKFYYDAGLMFSFFNRGLEFYIPFIMNK
                     ERDTPKRPKLGQRISFLVDMQKLTLHKQIRKMQF"
     gene            14478..15434
                     /locus_tag="PLS16_01735"
     CDS             14478..15434
                     /locus_tag="PLS16_01735"
                     /inference="COORDINATES: protein motif:HMM:NF040769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SelL-related redox protein"
                     /protein_id="HNJ59826.1"
                     /translation="MSFIYSRNSKIFKSILLASGLCGIVWGIIAIIYAQNWFDNLGHA
                     ELFQAMGLYELALGIAYLMASNNPLRNWQIVLIGFIIKITLTLGYLYYSLVKVEPDFI
                     FQMILITDLVWILPFALVLYNAYIHDQLLDNEMIGLQAASLNSIMHTMDTNKQNNLYK
                     LSEQQPVLLVFLRHFGCSFCRDTLMRIGELRKGIEDKQIKIVLVNMYDNATAEDNLKE
                     YNLADLDYIADEESILYKTFKLRRATFSQIFGIKVFIKMLKIFFIKGTFSSLTEQADT
                     FQMPGIFLVKNGNIIQQYNYTSIADTPPILEIIEDAALIQTN"
     gene            complement(15437..16351)
                     /gene="xerD"
                     /locus_tag="PLS16_01740"
     CDS             complement(15437..16351)
                     /gene="xerD"
                     /locus_tag="PLS16_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNJ59827.1"
                     /translation="MDSNVLWQSYILNFQAHLKIEKSLSINSIEAYLHDVELLKQYIS
                     IHHPELKIEAIDYKLLENFVAFLYDLGLNDRSQARIISGIKSFFKYLVLDNYLEKNPS
                     VLLETPKLSKKLPDTLSFNEIEQLIGALDLSKDENIRNKAMLETLYSCGLRVAELVNL
                     RVSHIYFDVEFIRVIGKGNKERLVPIGQEAIKHIQIYMDFIRNKLNIEEQYQDFLFLN
                     RRGKPLTRVMVFIVIKQLAEKINLQKNISPHTFRHSFATHLIEGGADLRAVQQMLGHA
                     SITTTEIYTHLDQEYLRTSLIMHHPRFQ"
     gene            complement(16351..16611)
                     /locus_tag="PLS16_01745"
     CDS             complement(16351..16611)
                     /locus_tag="PLS16_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59828.1"
                     /translation="MPFNILRIFDFIYAKEYIVIQMKIDEDAYKDNVLDIRMSSIVLK
                     VEAINKSVAIEKFKIATQKIRAIKKLEIECYELDKIPRISIM"
     gene            complement(17273..17746)
                     /locus_tag="PLS16_01750"
     CDS             complement(17273..17746)
                     /locus_tag="PLS16_01750"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNJ59829.1"
                     /translation="MQIKKVVFYTIIAALFVTACNINKPDEINNTASSEVDNLFNTTI
                     DSTKMDSLNQHKTTLKFEKELYDFGEVKEGTKLNKKVVYTNTGNNPLVIFMAFGSCGC
                     TTPTFSREPLAPGASDTLDIGFNSEGRNGNQSKTVKVYANTEPAFNEFKFTVKVN"
     gene            complement(17762..18082)
                     /gene="trxA"
                     /locus_tag="PLS16_01755"
     CDS             complement(17762..18082)
                     /gene="trxA"
                     /locus_tag="PLS16_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNJ59830.1"
                     /translation="MAKELTDSNFQNEVLDLQGVALVDFWAEWCGPCKLIGPVIEELA
                     GEFEGKALIGKLNVDDNRDVAAQYGIMSIPTLLFFKNGEVVSKHVGLASKGDLKKKID
                     SLLA"
     gene            complement(18156..18446)
                     /locus_tag="PLS16_01760"
     CDS             complement(18156..18446)
                     /locus_tag="PLS16_01760"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNJ59831.1"
                     /translation="MAKHQFSPLKDIFDEMIREMKIGDKMLELNIKKIWFEQMQGIIA
                     KNTKNIFFRENNLYITIESSVLKQELFMAREKICQIINEKLGKNVIKEVVIR"
     gene            complement(18450..19547)
                     /gene="recF"
                     /locus_tag="PLS16_01765"
     CDS             complement(18450..19547)
                     /gene="recF"
                     /locus_tag="PLS16_01765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HNJ59832.1"
                     /translation="MNFPAQIVSHIELLYFKQYLQQSFEFHHKLNIITGNNGLGKTNL
                     LDAIYYNCFLKSYFFKNDQFVFPFEKDKIQLNTNIQIQDNNFSIKVLNVKDSKKQAWL
                     NNIPIEKQIEISGRFPIVFIEPYDQDLIIGSAENRRKFLDATLALGNPIYLQKLIDYQ
                     KLIKQKNALLKQLPFSNSSETVLDIYNTKLIDLNTYIFKERIEFITQFNTIFKTKYKQ
                     IFDGDEHIQLEYESKFKGQNIDTLINQNKQDEILAQRTLIGIHNDDISFMMNNIPAKK
                     IASQGQQKTIVLALKFAQYEYIARCTKKLPLLFLDDIFDKLDMVRIQKILQIIHEENF
                     GQIFITYTDVNRILSILQKLSIKDFKHILIN"
     gene            19625..20305
                     /locus_tag="PLS16_01770"
     CDS             19625..20305
                     /locus_tag="PLS16_01770"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ59833.1"
                     /translation="MAKKQQESQALQKVNDFMDNIDLFIEQYKNIIIGVIVGLVLLFG
                     GLYAYKHFIKAPKEAKAKDAIFNAQYLFAKDSFQLALNGSGNIQGFKKIADEYGSTAA
                     GNTAKFYAGVCNLNLKKYAEAVKYLEDFSTKDPLVNARKYGCLGDAYAEQKKMDEAIN
                     NYKKAYAYDNEVTAPTYMYRAALALELSDKKNEALELYKKLNTTYPNSQEGQAAELSI
                     GKLEQQVK"
     gene            20409..20882
                     /gene="ribH"
                     /locus_tag="PLS16_01775"
     CDS             20409..20882
                     /gene="ribH"
                     /locus_tag="PLS16_01775"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNJ59834.1"
                     /translation="MASVLQNLSNYDEKSVPSGKGKKIGIAVAEWNKHITFSLLSGCT
                     DTLTKFGVNEEDIEISIVPGTFELPYAAKQLYDKNMNAVICIGCVIKGETDHDVYINY
                     SVANALQNLNIQYNIPFIFGVLTPNNEQQALDRAGGKHGNKGTEAAITALKMMQV"
     gene            21050..22465
                     /locus_tag="PLS16_01780"
     CDS             21050..22465
                     /locus_tag="PLS16_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HNJ59835.1"
                     /translation="MSKYTFKTSSKKIIADMLTPVSIYLRLREQFKNTVLLESSDYHG
                     DEHNFSYICFGAIAGYRLEKDVFSSFYPDGHTENIQTNSQMHFGILVNQFIKQFETPS
                     TNISDKIMTNALFGYMTYDVIEQFEDIQLRHEKPNIPTAQYYAYQFIIAFNHFNHELY
                     IIKNAIEGIESKYNFSLDAIETMIKNNEFNLGHFSSIGEKTSNVDNETYIEMVKRGIA
                     HCYRGDIFQIVLSRRFQKEYEGDDFNLYRALRSINPSPYCFYFNFEDFRLIGSSPEAQ
                     LIIKNNKVTIHPIAGTFKRTGNDVQDANLAKQLSEDEKENAEHIMLVDLARNDLSRNG
                     KNTVVEVYKEIQYYSHVIHLVSKVSSFIDDTADKIKIFGDTFPAGTLSGAPKYKAMQL
                     IDKIENTPRSFYGGAIGCLGFNGDFNHAIMIRTFLSKDNLLTFQAGAGVVADSKPESE
                     LQEVENKLNALNKAIDLANTI"
     gene            complement(22526..23698)
                     /locus_tag="PLS16_01785"
     CDS             complement(22526..23698)
                     /locus_tag="PLS16_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNJ59836.1"
                     /translation="MISEKYNQRGVSATKDEVHAAIKNENKGLFPQAFCKIYPDYLTN
                     DEQFCSIIHADGAGTKSVLAYIYWKETGDISVWKGIAQDAIVMNTDDLICIGATSNFL
                     LSSIINRNKHLITGEVIKTIIDGTNEVVEMLNEHGVTVFPMGGETADMGDVIRTVSVD
                     AVMTARMHRNDVIDNANIKAGNVIIGLASYGQATYEKEYNGGMGSNGLTSARHDVFSN
                     IYAQKYTECFDPNTASEFIYQGTKLVTDKIEGTPIDAGKLVLSPTRTYAPVIKKIIQT
                     IGTKNINGLIHCSGGAQTKVLHYIENLHIIKDNLIAVPPLFNLIQSESNTNWQEMYQV
                     FNCGSRMEIYCNEQFANEVIAISKSFNIDAQIIGRVEPADSKKVSIISDKGNFEYH"
     gene            complement(23764..24207)
                     /locus_tag="PLS16_01790"
     CDS             complement(23764..24207)
                     /locus_tag="PLS16_01790"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HNJ59837.1"
                     /translation="MESLFEKSTQQAIAQRIQNLNANSKRAWGTMTVAQMLKHLEIAF
                     AVPINKVSLPKENLQFLVANPLMRKLVIDIIPWPKNMMTAKDFIVKTDPEFEQAKQSF
                     LEVYKQFIEAKNLTGAHPIFGEMDKDTWGRAMYKHLDHHLKQFGV"
     gene            complement(24274..25467)
                     /gene="argG"
                     /locus_tag="PLS16_01795"
     CDS             complement(24274..25467)
                     /gene="argG"
                     /locus_tag="PLS16_01795"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNJ59838.1"
                     /translation="MSNNKVVLAYSGGLDTSYCVKYLSQDLKLDVYAITVNTGGFSVD
                     ELKGIEERAKYMGVKEYVVADIIQNYYDNCIKYLLFGNVLKNNTYPLSVSAERVFQAM
                     AVADYAKKIGAGYIAHGSTGAGNDQVRFDMIFNIVMPEAKIITPIRDNKLSREEEIEY
                     LKQHGIEIDAKKAAYSINQGIWGTSVGGKETLTSRNTLPESAYPTQLEENTPIDIEIE
                     FKQGEPIALNGKAYNHPTELIIELNKIAAPYAIGRDVHVGDTIIGIKGRVGFEAAAPL
                     ILIKAHHLLEKHTLTKQQLFWKDQIAQVYGSNLHEGLFYEPLMRDIEAMLSSTQKCVS
                     GKVFVQLLPYRFNLIGIESKHDLMSSKFGSYGEMNNSWSGDDVKGFAKIYSNQMMIWK
                     KVNDQ"
     gene            complement(25490..26203)
                     /locus_tag="PLS16_01800"
     CDS             complement(25490..26203)
                     /locus_tag="PLS16_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ59839.1"
                     /translation="MKALKPKFSIIVAEEQHAKYVHELCKLYEESAKKRGVGISKRKP
                     EYLEAKIKEQKAIIALTHEGGEEIIVGFCYIETWTGKNYVANSGLIVKEEYRKNGLAK
                     KIKKFVFEYTRKKYPNAKVFGITTSLAVMKMNSDLGYKPVTYSELTRDSAFWDGCMSC
                     QYYDVLTRTGRTNCLCTAMLFDPKASKKKEFVATIKKVDKKIPQKLKSPKAQRIKEVK
                     TAGRSKANKKKTEDKDNSN"
     gene            26324..27163
                     /gene="lgt"
                     /locus_tag="PLS16_01805"
     CDS             26324..27163
                     /gene="lgt"
                     /locus_tag="PLS16_01805"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNJ59840.1"
                     /translation="MTLFSLFVFDWYAKPEIFMITDNFGIRWYSLFYGMAFFIGYYIL
                     SWMFKKENKSIEDADSLLMYMFFAVLIGARLGHVFFYDWPYYSQHLSEIPQIWKGGLA
                     SHGAAIAIPIALYLYKRKHPDQTYLWIIDRIAVPLALGSSFIRFGNFFNQEIVGNYTD
                     GSWGVFFHRHIDELTGMLDTGPRVPVQLFEGFLYVFLFIVVLTYYIAKKYKPKEGSVM
                     AIFLFIMFIGRFFLEFWKDGEKIVHLGSIELNRGNVLSIPFIIIGFIVFFISTRKKDA
                     VKI"
     gene            27249..29096
                     /locus_tag="PLS16_01810"
     CDS             27249..29096
                     /locus_tag="PLS16_01810"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HNJ59841.1"
                     /translation="MIKKVFAIVFCILLSYTVFAQAEVINRSAADSLLAPFYHGVASG
                     DPTPNSVIIWTRLTTYDEGILSGTWKVATDTNMLNVIQQGVYSTDSSKDYTVKIDVGG
                     LEPGTWYFYEFEYKDRFSLRGRTKTAPVGGVEQLRFAFASCTNYPAGYFNVYDRILER
                     NDIDAVLFLGDYIYEYGPNQYGSTRKDVMPTKEIVKLEDYRLRYSTYRLDPALRKLHQ
                     QFPFFTVWDDHEVANNSYKDGAENHTPGVEGNYYVRKSAAQIAYSEWLPIRLPEESNP
                     SKIWRSYKYGDLAEIFMLDTRLYDRSYEKMNKNDTTKKLLGNEQMEWLKNGLINSTAK
                     WKILAQQVMMTNLDPFGLVLNGDQWDGYPLERKKLYDIILDNDIKNVITLTGDIHTAW
                     AMDLPYDKTKYDPKTGKGSVGVEFVCTSVTSPSVPFPLDPLYPIVYGVLPHIKYTDLH
                     WKGFSILDLKTTQAQGDFYTVSRIDKLDAGKKYVTGYYTLDNTRWLKKANQETNKIVP
                     NIYFSPFYPRLASQTPIKNNLKSGVIIGVYPNPFIQDIQMQFNLFHTNPVMIQIVDMN
                     GKMVFQKDMGTLNNGLHNEKITVENIPAGVYQLVIRSGNDIIAKSIQKF"
     gene            29108..29722
                     /locus_tag="PLS16_01815"
     CDS             29108..29722
                     /locus_tag="PLS16_01815"
                     /inference="COORDINATES: protein motif:HMM:NF009807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HNJ59842.1"
                     /translation="MKKTKASASLSANADKTKVKKVAPEKKIIDTKIYPIGIFEFNEQ
                     IAKENIETYIEEIKELPKNLKKVLKQVTKHNKENSYRDEGWTIEQIVHHLADSHINAF
                     IRFKLALTEYFPTIKPYNQDLFAETSDVFLSSLKSSYHIIDGIHKRWTILLENMDNED
                     FKLGYIHPEDNQKHFLLKVLAMYAWHGKHHIEQIKVALGSDSED"
     gene            29794..31680
                     /gene="yidC"
                     /locus_tag="PLS16_01820"
     CDS             29794..31680
                     /gene="yidC"
                     /locus_tag="PLS16_01820"
                     /inference="COORDINATES: protein motif:HMM:NF002356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNJ59843.1"
                     /translation="MDKNTITGIVLIILIWVAMSVYNSKQQEKFETTQKIEQKSEKKD
                     TSVASLPNIDSVQKNAVDSSVDNKLDLVEYGVFGAAANGTKQEIIVENEDCIFTFSNK
                     GGVLTKIELKKFKTYDHKPLILFNQTPEDFNFTFVVNSAKIPIQTSQLFFTPNNGNKV
                     IKGNEIYQLDYTISDISGKLYSQSYTISGKGYVVGFVADAKGLSKEIPQSANIIPLAW
                     SQEIQSHELNHKEEKMSSGVYYQDIDKNVDDIGLMKDKDEKIEKAINWVSLSQKFFNT
                     TLIAKNQPFLANSTVKTIAGTDDSSALVKKLFVELKVPFNNETNYQFDGKFYAGPSSF
                     KELKKVESGLQQIIPVGGSILGWVNKFLVIPIFNFLGKFISNYGIIILILTLIIKLIT
                     LPFTYKSQLSMIKMRVLKPEMDALKEKYGKDQATFGAKQMELYQQTGVSPFGGCIPML
                     LQWPFLIAMYRFFPSAIELRQEKFLWANDLSTYDAVIYLPFNIPILGSHLSLFAILGV
                     VTSFGMTLYTMKMQPQQTSGGGEFAEAMQKQMKVMQYFFPFMILFFFNSSSAALSYYF
                     FLYNGFSLLQQWIMSKFFIDENAIRAQIEHNKKNPKKKSNFQTRMEEMMKQQQALKEQ
                     QKKK"
     gene            31739..33229
                     /locus_tag="PLS16_01825"
     CDS             31739..33229
                     /locus_tag="PLS16_01825"
                     /inference="COORDINATES: protein motif:HMM:NF014214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HNJ59844.1"
                     /translation="MNVRNLLLVLLSIIFFSTNVIAKSDVNINIALYYIKNNYKEIGL
                     EELDIKDLKLKDIYFDDFSSINRIWFQQYNNYLPLKNGFIGVHIKDGQVVQVTNNGKA
                     NLKLNSQNLIVSIDNKTAILSAIAKVLPKSELENLKQTSSDKRVQKYAYTANDVEAID
                     DNIDTYLSYVLDENNKIDLAWIVNFGTNHKNYWEIEVSAVSGKVIRIQDRVLHCSFGK
                     GGFLESISDEEIHSEEIHNHSNSNQRTEGAPSAAYRVFPFSKESPIYTPRLLIKNPSD
                     DIASPFGWHDANGQDGIEFTITRGNNVYAYADKAGNNKVTPGSPPDGGANLNFDFPLD
                     FSKRLDTLDNSKAVITQLFYSNNVIHDILFNNGFTDANRNFQAKNYASGNTATEKDFI
                     WAEAHDGAGENNASFYSAVDGSNSPVAGGRSRMQMYMWSDISNYNLILSRPSGIIDTL
                     RCGTQSDWGVCNFDITGAVKNAFSGSVSLPSYVCSAVSNSAEILEK"
     gene            33235..>35152
                     /locus_tag="PLS16_01830"
     CDS             33235..>35152
                     /locus_tag="PLS16_01830"
                     /inference="COORDINATES: protein motif:HMM:NF014214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HNJ59845.1"
                     /translation="MDRGDCDFSAKVYNAQQAGAIAAIVVNRQSAGDTIINMAAGTSA
                     NLVIIPAFFVTYADGQKIRQYIDSGNVIRMNKTSLGNCLRKDGSLENAFVAHEYAHGL
                     SIRLTGTGVNCLNNKEQGGEGWSDFIALALTVKPSDNKNTARGVGNYVVSEPINGKGI
                     RMYPYSYDMSINPLTYGSLMDMDGTPILDSEGEFIGYVPNSVEVHDMGEVWAVTLWDM
                     YWNFIEAYGFNPDFYNGNSGNNKAIKLVIEGLKLQKCSPGFLDARNAILKADSIMNGY
                     ANKCLIWQSFARRGMGWSAIQGSSDHAKDQTEAFDMPPSCNSNPIPNFTLSDTSICLG
                     ELIQLTNTTTGNIDSLRWKMIGGSPVSSNETTSLSVNYPSAGEYFITLYIYKNSYIDS
                     ITKKVKVYNIPVITTNISSSTICPGSSVTLSASGASTYNWSNGANNAQISVSPTVNTV
                     YKVVGKSNGCISDSVAVSIFLKTKPTVSINASSLSICVGETDTLTASGATSYIWRDGS
                     TSNSIVVNPTVTSSYKVIGILNGCSSDSVEIQITVKPKPIVSISPTNTTICAGQSVTL
                     TASGASSYVWSTGQSGNTIVVNPSVNSTYKVLGITNNCSSDSATAIVNITTTNPTVSI
                     TKSPNVSTICEGDSV"
BASE COUNT        12162 a         5152 c         5343 g        12495 t
ORIGIN      
        1 aacgtatcat ctaaagaaga tttgttttta taatgtaccg caaagttact taccatagca
       61 gaaattgcac caatggcaat actttcacct gtgtttacaa aaccacaagc aggagtaatc
      121 gctaccaagc ctacaacagc tccaacgcaa gctccaattg ctgatatttt tttacctaat
      181 aaggcatcta agaaaaccca agcaaccatg gcagcagccg cagccgtatt tgttgtagca
      241 acagcattag cagcaactgt gttacaagca agtgcagagc cagcattgaa accaaaccat
      301 ccaaaccata gtaaacctgt tccaattaca attaatggaa tattagaata ttcatgtgtg
      361 cgttctgatt cagttcttgg tcctaagaaa attgcaccag ctaaggctgc aaaaccagca
      421 cttaaatgaa ctactgtacc accagcaaaa tctaatacac ccattttaaa gaatattcca
      481 tcagcatgcc aagtcatgtg gcaaagtgga gcatagataa agatgaaaaa tagcacaatg
      541 aaaattaaat aagctgaaaa cttaacacgt tgtgcaaatg aaccagtaat taatgctggt
      601 gtgataacag caaattttaa ttgaaacatt gcaaatagaa taaatggaat ggttgaacct
      661 tcttgaggtg tcaatgttac gcctttaaac ataaaatagg taaaaggatt tccaatgata
      721 ccgtatccgc caacatcttc tccaaaacat aagctaaatc caaaggctac ccaaatcaca
      781 ctgatgatac ctaaagaaat gaaactttgc aaaaccgttg aaatgatatt ctttttatta
      841 gcaagtccac cataaaataa cccaagaccg ggcgtcataa tcataactaa tgcggttgca
      901 acaatcatcc aagcagtatt gcccgtatca attgtactag cagtacttcc ttgtatagat
      961 tgtccagggt tgaaggctcc tgcagctact attgccatta ataaaagaaa tggtaataaa
     1021 aacgattttt gtagtctcat aaaattaatt tttaatgttt tttaataatt ttaatcgaat
     1081 ttatatcttt tcttcaaaaa tagtttaatt atttttagca aaagtgtcaa aataatgatt
     1141 ttttaaaata aaaatgcaat aattttaact taaatcgtaa ttttttggct tttataagga
     1201 aaatacttgt gttttttttc tttaaatagc aaaagctgaa ttagaaccaa atcaagattg
     1261 gtaatttttc ttaaattaaa ttagaattgg caaaattaca gtaactttgt tgcttaattt
     1321 atagtatgga aaagtttaaa gtagaggtgt ggagtgatat agtttgccca ttttgttata
     1381 taagcaaacg taattatgaa aaagcattaa cattatttga attttcagag caaattcagt
     1441 tggaatttaa aagttaccaa ttagatccag attttattca aaatcaagat gaaaaatatg
     1501 atattaaagc ttatttagca aataaatacc gtcgttcatt agaagaaatt gaagacatgc
     1561 taacacaaat tacatctagt gcaaaatctg ttggcttaaa ctacgatttt aataaagtta
     1621 ttagatttaa tactttacaa gctcatagaa ttttaatgaa ggcacaagat gaaggattag
     1681 gaaataaatt aatggaggta ttattttcag cttattttga acaaggatta gatttaggaa
     1741 atactgaagt tttgaaaagt atagctttga aaagtggatt aaatgaacca gcgtttgagg
     1801 aagcaataac tgatgaacaa tttgcatacc gtgtaaaaca agatattcaa gaagcagcaa
     1861 atttaggcat ttcgggtgtt cctttctttg tttttaatag aaaatatggc gtgtcaggtg
     1921 cacaaccagt ttcggtattt tcagatactt tagaaaaagc ttttcatgaa tggaaagcaa
     1981 gtactgaatc taaaattgaa aatataggaa atggtgccac atgttctatt gatggaaatt
     2041 gtgaatgagt tttaatagga taaaatattt tagggatatc aggttctaaa gaagtttata
     2101 gtcttttgcg caggatatat atttgatggt aacttttgga acagtaaaaa aaacgaatta
     2161 actgatgatt tattattcat tcacattaat tcacctgcaa aatcatttat gagcagaatt
     2221 ggaaacatta tgacacaact tggaatggaa gaaaattttg tagaaatagt attgaagtaa
     2281 aaaagccgaa cagtaaatat tatgacgaat agaagaaagt cctgaaaagt aaagtgtctt
     2341 gtcctgtaat acgtgtaatt cctactcaat tttggatagt gtcttttttt acttatcttt
     2401 gcaattcaaa taggcatact ttttgtagtt attgataaaa atgttctttg atttatgggg
     2461 ctgttcggtt ttgacggcag atgttcgttt aagtaagcat gtcgagtatt gtacaaattg
     2521 actcgttaat cctaaagtac ataacttttt taattggcga gagatctttc gctttagcag
     2581 cttaatacaa ggtattaaca cgctaatgct cccacgtttg attttcttgt tgtcagcgtc
     2641 tggagtatca ttaataacaa gatagtttgt tgatgtttct aatgcaaatg aaattataga
     2701 aactaaggtt taatttggta agtataaaca cctaattatt cccgacaatt aagtttgtac
     2761 tacacatgta gaaagcttat gggtgctttg tccggacgtg agttcgattc tcaccagctc
     2821 cacatttttt tctcttatgc ttaaattcct ataaaaaaaa ctatatttat tacaaaaaaa
     2881 ataataaata tggatttaca gcttagcata acctataata ttcttttatt tattttttct
     2941 gctattgtag tttgggtttt atgcaataaa ttatcagaaa tagttgatgc tattgacctt
     3001 caatttaatt tgggtgctgc atttggtggt acaattatgc ttgcaatagt tacgaatttg
     3061 cctgaaatcg ctattgtctt taatggtgct ttaaatggca gtattgattt ggcagttgga
     3121 aatattttag gtggcattgc aatacaatct actttattga ttttatttga tttcgtgtcc
     3181 agaaaacgag aaacgctacc attttctaca cttgctagtt cagaaacaag tattttacaa
     3241 ggattatttt tagtagccat actttctatg gtgcttatag gcaaacaatt tcctgaaagt
     3301 tttatcgttt ttagaacgac acctcctgta ttttttattc tgatactctg gatactaagt
     3361 attttagcaa tgaagcaatt tcaaaattct aaccttttaa agaatacaac gattgaagaa
     3421 aaaaatatgc catcaaaata tagcaaaaaa tccgctttaa cttggttgtt tataatttca
     3481 gtatttgtac ttttctttgg tgtaattctt gaaaatacga gtaatgccat tgcaaatcat
     3541 tataacatta atggcgtaat atttggagca acagttttgg cttttgtaac ttcacttcca
     3601 gaaatttcgg gtggtttagc atttgtaaaa tcacgctctt atactccaat cattgatgat
     3661 atttttggag gaaatgcatt tcttcctgta ttatttgtgc ctaccatttt cctaactaac
     3721 aatgctattt taccaaaagc cgataggata gatttatacc taactggagt cgctatgctg
     3781 attactataa attatttaat tggtatgatt ataaaaaagg agcaacgtta ttttggtatg
     3841 ggtatggatt cttggattgc gttaagtatt tatttattat ctgtaattgg tttatttttt
     3901 atataaatct acagattcaa atttctattt tattccctta ataaattctt gaatactttc
     3961 tttcaaatta gaagcttgtt caatatgttt tacaaatgca gagccgataa tagcaccgtt
     4021 tgcattttta caagctagtt caaaagtatt tctatcttta attccaaaac caattatagt
     4081 aggatttttt aaattaagct gcttcaatct attaaaataa gttgattgtt gagcctctaa
     4141 atcaatattt gttccagtaa tagaatgcgt agatacaaca taaataaaac tatctgaaat
     4201 gctatcaatt tgcagtaccc tttcattgga agtttgcggt gtaactaaaa atatattttc
     4261 aataccattt tctttaaatg atgtttgata catattttca tattcataca ttggcaaatc
     4321 tggaataatt actccatcaa taccaacatg agcagcttct ttcaaaaagt tttcaaatcc
     4381 atattgtaaa gctgggttga tatagcccat taaaactaaa ggaatatgaa ttttttctct
     4441 aatattttta agttgattaa atagcttttg gattgtcatt ccattttgaa tagcaatggt
     4501 attagaatat tgaatcgttg gaccatctgc agttgggtcg gagaatggca ttccaatttc
     4561 aaccatatcg acaccatttt cttgtaatgc ttctaaaatc tgtggtaggt cgtttaaatg
     4621 aggatatcca gccgtgataa aaatgcttaa tatattttct tttttctctt taaagagttt
     4681 ttgtattcta ttcatttttt aaatacaatt atttgtagct attgataaaa gacaaggcat
     4741 ctacttgaaa attaaagaaa tcttcagcat gcggtatatg tccagtttgt atatattttg
     4801 tagggttata agttacattt gaaccagttg ccaatttgaa ttttagagct aaattatctt
     4861 tgggtaaaaa catatcttgc tcaccccata ctaataaaat tggtaaatct aatttcgata
     4921 acaaaggtgt catatccatt tctgaaaaat aggaattatt atattttatt tgagcataag
     4981 cattgagttg gttcttgtaa attacatcta aaggtgtact cggagcatca gtttttcttt
     5041 gttttacatt attcagattg aaaaataatc cattgttttg catcaaaaca tttatatgct
     5101 tttcaaatac ttctgagtta aaaacaggat gttcagtata ataattttta agatttgtcc
     5161 aatagcctcc agggttatta tttgtagcaa ttctttcatc tgctaaatct ataactttct
     5221 gttttgataa agctaatcca ttttttaaat cataagcagg agcttccaaa ataagtccat
     5281 taattttttc ttgtcgtgtt gaatctgacg cataaaaaca agcaactgca ccaccaattc
     5341 ctgaaccatg tagaataaca cttcttactt tagcaatttt aaaagtaaaa tcaatgactt
     5401 tatctaaatc agcagcaaat tgctcaatac tcatctcact tggctttgta ttaccttgtg
     5461 ctgttcctga gcctctttgg tcataataaa ctactttata tttattttca atatactgcc
     5521 aatacacatt atttttttct gtatttacat ttgaattcag tccgtcattg atataaatta
     5581 cagcatgttc agcaatttta ttacccgtga tgttgatagg caatgcaaca tcatcaattt
     5641 ttaaaaaata atagttatta aactgattgg aattcttatc ctttttgcaa gaaaacagta
     5701 agaatataaa tgcgatatag gtaagttttc ttatcatttt tattcaatat ttcttctaat
     5761 aatataggct acacctgctt gtacaatgat atgtcttgtc cagttatggt tgtttggaaa
     5821 attgtaatac aaaccgatac caccaaataa attcaatggc gttcgtccac ttttgctaaa
     5881 atctaaacca gcattaaaat taccaccaac aatagcattt gaaaaaccta ctttaatttt
     5941 gtttacttct ccagatggtg taacttcata agtaggcaca tttaacttgg catagtaata
     6001 accaccacta atgctaattt ctgtatagaa aatcttaggt gtagtgtgac gatatacaaa
     6061 agaaggcata aagtacaaac ctgtttggta tcgttctcgg tataaaaatc caatagttgg
     6121 tttgataaat aaatctctat ctgccacttt tatatcataa gctgattttt tttgaatttg
     6181 ctttgcttta gaaaacaatc ggatataacc tccgaattcc attccaaaat tgatagaaac
     6241 aggttttata aattcgatat ctgcaattcc agcaacagaa ataattgtct tcttgtattt
     6301 caatccttca acaggtaata aatttttgta tttaagtgtg gcatcttgag caaacaaact
     6361 accaaaataa aagcttagga atatgaaaaa tataaatcta taatttgagt tcatctacta
     6421 gtttaaaaat gtttatgaaa atacaacaac ttactgacta aaaaaaaggt tgtaggatta
     6481 ataacgatta tttttaacac tatttttaat gtttcattcg ctgtgcgagt attttatcga
     6541 tatactttcc taaaatatca aattctatat tcacttctgt cccaactgta tattgatgaa
     6601 aaattgtatg tactaaggta tatggaatga tagcaactga gaatttattt tctgctgttt
     6661 ctacaattgt gaggctagta ccatcaatac aaattgaacc tttatcaaca acagtaaaaa
     6721 gaggattttt tacaaattca aaatagaaat atttactgcc gtctttatct tcaatttttt
     6781 cacaaatagc cgtctgatcg acatgtcctt gcactaaatg tccatctaaa aatgcatttg
     6841 ccatcattgc tttttctaaa tttactttag ctccaatggt ccaactattc agcgttgttt
     6901 tgtctaaagt ttcttttatg gctgtaactt tatgcgtatc accaatttgt tcaatgactg
     6961 tcaagcaaac accattgtga gcaatacttt ggtctatttt taattcattt gaaaaattac
     7021 tttttacagt aaaatcaata ttagttcctt ttttttctat ggcaataatt tcgccgattg
     7081 tctgtataat tcctgtaaac atatctatct aaacaaatga atatagccac ttaatggttc
     7141 gacaattttc tcaacaccat tggctgtttt ataatacaaa taacatgtat aataatatac
     7201 agcagtcggt aattcttttc catttttaat gtccgtccca ttccaattaa tatcaggatt
     7261 ttcagtttgg taaactaaaa ttccccatct atcataaatt ttaatatcaa ttttttctac
     7321 acttctatat ggaataattg gcgtaaacca atcattttgt ccatcattat ttggtgtaaa
     7381 agtattgggc aattcatatc ttggacaatc gttagaacaa accatattgg acatagtttt
     7441 gttgccattt ttaccaacag tttctaattg ataacatgct gctaaagatt gaacagagtc
     7501 caaacgatgc gtaaatgcta aaagtccatt ttttggcatt gtatctaata atacataatc
     7561 attgtaattg ttggctttat aataaatatt ggttttaata atggtgcttg tgtcgcatgt
     7621 ggggtcaaaa ttccaaacta aatagtttac aaaatcattt tctggcagat gtctatctaa
     7681 acagaaatta ctcaaattca ataaaggttt acatgggaaa gttgtatcta tcggaaccga
     7741 acaagcctct tgcgagaagt ttaataatgg tgtttttaat ccatcaatat tataggtgcc
     7801 aatagatttt attttataac aatatagact atcatttagc aatccaacat ctttaaaatt
     7861 attaactgtt gttgttgtta cagagtcaaa agttgagcgg cctgggtctt ttcgatacac
     7921 tacaaaacta tcattttgcc atggcgtgtt aaaatcccaa tgtaattcaa tgcttttata
     7981 ttttggtgtt acatttagga atacgcttgt tgcttcattt gtaaaaccta atgtatccaa
     8041 tccagcattt gctataaatt gaattttata tttgtaagaa agaaattctg tattcaaatt
     8101 agtatctaaa taacttgtat cattaattgc agaatatgaa gtaaaagttt tagaatgaat
     8161 tacactataa ttttggctat ttgcatcagc tcgatacaaa gtaaaggtgt aataaccttg
     8221 attgattaag gtatctaatt catcagcatt tgggcgagac cattctacat atatttgacc
     8281 atttatttca tttgttgttt ttacatcaac attataaata ataggcaaac tcaaaggcaa
     8341 acgcacacaa acttcatcag aaggcaaact actaaaagga tttacttcaa tatttgttgc
     8401 cgttaaatcg gcaaactctg ctttgatttt ataactgtat tgttctccaa tttgaaccgt
     8461 attgtccgta taagtatagt ttttagtata gccaataaat gtataacctt gtgcttccaa
     8521 gtcagtacta caagagtcgt taggattgcc acaagacttt ttacgccaaa tagaaaattt
     8581 attaaatttt ggattagtac tacaagaata tggattatcc caatttaatg taaccgtatt
     8641 tgctgagaca tcacctattg ctgttaagtt ttgaggtgca ggtgctaaaa cttttataaa
     8701 tactgttttt atatctacca atggtggact aacaaaatca tcttcagctt taaataaaat
     8761 gggatattca tttttgttta gatggctaca atctgttttc catttaaaac ttccagaaat
     8821 tggattttgt ggtgtttgtg tcgtaaaaat ggctgcttgt gtaggtaaac tatacacaat
     8881 tccattagaa gttaaagtaa ttttttgggt aacatctgca tctgttgcac ttatagaaaa
     8941 atttacggaa tcacctgcaa ctacgcaaat gttttctgga atggtcaaaa caggtggtct
     9001 attacttgtt gattctacaa atatttgcat atcacgaaca acagtaccaa ttaaaaaacc
     9061 tgctctatat tcttgaacta aaattgcaat attataaatt ccttgaactt gtgggtattc
     9121 ccaaattaac tctccagttt tgggattgat tttaaattga ttattaattc ctgcttgtat
     9181 ttgatttgga tataaataac cgctcacatt cgcagactga ccttgttttg gaggaatgag
     9241 tgaaaatacc aaactatcac catctgggtc aaaagcattt gggttatgga caaaacgttg
     9301 accaacattg gctgcatcaa ctggaggatt atacaatatt ggtgaactat tgtaacccat
     9361 atcatttgca ttaaatattc taacggtatc ttcaatataa aaaatcgtat tgacagagtt
     9421 ttgcatgtta ataatgttag caattctatt tgggtctgtc attgaaacaa tataacgagc
     9481 tggacctgta taagaatgca acattctata tgtattttta aagacatcag gaaatggtgt
     9541 tacatctctt gatattctca cgatagaatc tttagtacca tcgccccaaa agatttctag
     9601 ttttgggcga tctgcacctt cactttcccc acttaatttt gtataagtta caactgtaaa
     9661 ttcataagta taagcggcaa tatgcctata cataatttct ccagctctat tatgtgttgc
     9721 aaaaatattt tttgaaacta taaatagtat caaaatcagt aagattcttt taatgtttat
     9781 ctgcataaaa tactttgtaa atatacatag ctttttgtga atataaaata ttcctttatt
     9841 ttcagattaa tagaaaagat acgattaatt aaataatctt ataccgattc gcacgtttaa
     9901 atgtgcctat atcattaaaa cgtacaccat tttcttgcat tgcttttaat gctcttgcac
     9961 gtgcaatatg tcttatataa aaagtttcat tgacaacaaa atgatgtatt gcatgtgtcc
    10021 acccaaaaaa gaagcagaaa atctgtaatg ggaaagtcca ccatatattt aaaacttgtg
    10081 tttgttgcat aatattacca ctttcaacat ctccaaaata gtgcatatta gaagtaataa
    10141 aatgcaaaca aaattgacgc aataaattgg gtgcaataat aatagctact aaaggtttta
    10201 tccaaataaa ttgattagcc ataaataaag gtgtctggta gtcaatatta aaaatttttg
    10261 taaaaatcac aattaataaa tgcactaaat atacatacca aattgaatag gcaatcaaac
    10321 caaatggcaa catagaaatt gttttaatct tcttgaaacg caaagcttgt tctttactta
    10381 aatttccatt tctgacttca tttctaatat ctttaactaa aattttggct ctcataatgc
    10441 caccaaaaat taaatctggc gtaccaaaca atctttttaa tgaccatttt tcgccatttg
    10501 taacaccacg ttcttctaca tcgtgcaaag ttcctgaata tttatgatga tgaaaatgta
    10561 attctcttct caaccaagga ttgatagtta atggacgtaa aatccaaact gaaaataaca
    10621 taatatcttg tatgattttg ttttttctga aatacatcca atggattaaa tcatgttcta
    10681 attcatgtaa aatgcccatc cagaacgcat tgaatggaat taaaatccaa acagataaat
    10741 gtccctttat ataaaaaact gatgctaata caatcataga aatagataac aaaaaaaatg
    10801 tcattcctat ccaattttga tgtttcagca acggatattt ctgtttcaaa tctttatacg
    10861 attgactaat ctcttgtcgg attagcgtaa ttttttggct atctgtcagc ataatagttt
    10921 ctaagataat aaaaataaaa ctaactattc caaatctccc aacttctttc agcttgaaga
    10981 tggagcattt ctaaaccatt ttttgttttt gctccttttt ctattccttt ttttaagaat
    11041 aaagttactt caggattgta aactaaatca tacaataaat gttgcacact tataaattga
    11101 taaggaattt ctggagcagt ttcaatattt ggatacatac caattggaga agtattgatg
    11161 atgacagtgg atttattcat caattgctca tcaatttctt gataagtaat tgtttgtttg
    11221 gatttagttc tcgaaacata ttgaaagttg atattcaatt gttctaaaac atattccact
    11281 gcttttgaag ctccgccagt tcccaaaatc aaggcaaaat tatggtgctt ttctaacaaa
    11341 ggttttaaac tcatttcaaa accataaaca tcagaattat aaccaatttt cttgccgtca
    11401 ataaatttaa tcgtatttac agctccaatt tttgcagcag cttcatctaa actatctaaa
    11461 aatggaatta cttgttgttt atagggaatg gtaacattta atccaaaaag attgggtttt
    11521 tcattaaaaa gttgctctat taaatggata ttttctaatg gaaaagtatc ataatgagca
    11581 tcggtaatat tttcgttttt aaacttttca gcaaaatatc ctttagaaaa agaatgggac
    11641 aaaggataac caattagacc aaattgtctc ataaatgagt atatattttc aattgttata
    11701 aataatgaat aataagcatt tggaaatcag tattatcttc taattcttga tggaatttat
    11761 aaatatagtc tgaatttaga ttatcttctt ccattagata agtaagcttg agttcacact
    11821 cttttacttt attttgcata aacaatacag caatttcggc atgtattgct tgagtatatt
    11881 ctttacaagc ttttttacag ttgtgtaata tttctaatgc cgttccaaaa tcattcaaat
    11941 taaagaatat ttttgccaac aaaatataag cctcttcttt attaatataa ttttcaatcg
    12001 cagcttcaca agcatcaata gcaatttcaa tttcattaat ttccattgca gattcaatta
    12061 aagcaatatg aaaatcatac atctcttttt ctaattctgc agctttttgg aatgattgat
    12121 atgcttgttc ccaaagtcct tcttttttat aagtttctcc aatcttataa taaataaagc
    12181 caaaagccgg ctccaaatgc aatgcttttt tataataata tctcgctagt ttgtaattgc
    12241 ctaactcctc aaaacatttt ccatgtaata aaaaaacctc ttcatctggt tcataaactt
    12301 ccgtcaaatc attgattaca tctaatgctt tggtaatgtt tttttctttg attaaaagct
    12361 caatcatatc aatataagca taatcttcat cttcgctaat ggcaataaca tattcaaaag
    12421 cttctatagc tttttctgtc aatccaatac ctcgaaaagc acaacctaaa ttataccata
    12481 aatgatgatt atatggatac atatcaatca tgtgttgaaa aaatgtaatc ccttcatcgt
    12541 acaattgagt aacatcaata caataattta ttcttctgag tgcttcttgt tcatctggat
    12601 taaggataat acatttcttt aaatattcaa aaacatggtc atatcttgat gtttcttcgt
    12661 aaatatcact gatttgtaaa caaatatcca ctaaatcctc actttcaatt gtattttcca
    12721 gcttttttaa aatatccaat gcttctttgt atcgagattg agcagttaat atttctgctt
    12781 ttaaaatata ggaaccaata tccgtagcat caaggctttg gattaattca attaattcaa
    12841 aagcggttgc cgtatctttt aattgaaaag ctaaatttgc ttttcttaaa attaaggaag
    12901 gtgagtttgg atgaatttgt aatgcttgct ctgttgcacg caacgcttta tcatattgag
    12961 caatctgttc ataataatca atgatgcttt caaaatcatc ttgatcaaag aatgctggct
    13021 tattttgctt tacagcattt tcaaattgca ggattaattc tctaatttca tccgcatttt
    13081 caaattcttc gttatcaaaa tcaaaaaaat ccatagattt atacaaagtt aagtttaaat
    13141 tctcacattc aaaatagagc ttattaatga aaaattacca aatagttata cacatctttg
    13201 cattactttt tcttaatgca agttttatct atgctcaaga ttctttagaa acaactataa
    13261 aacatccaaa actataccaa atacaacaga aatatttgaa ttggattgat aaaaaagata
    13321 aagaaaagcc gtttgccttg cccgtaattg ctggaaataa gtatgacggt gttcaaattg
    13381 gtgcagcact gataaattta agacagccag taaaaaatat agatttttca ggtgttttat
    13441 tatatggaac taaaagtaaa aaaataaatg gcattgctga tattgcttat tatatcgaac
    13501 cgaaaaatga agtacttaat aaaattaaaa ttgctacaaa tttcaaatct tttagcaatg
    13561 cgattagccc aaataaatta aaatattatg ctctaaaacc ggaattggaa tttcaattat
    13621 tttctaaaaa aaatagcaaa ataacacaca ccatcaattt taaatctaat ttaatatttg
    13681 agcaaggaaa agtctatttt agtttcaacc acaattcaga cagtacttta aagcgatttt
    13741 atgccaatga tttaacttat gcttttgatt tcaaaaacaa aaattatcct ttcaatattc
    13801 aagtacaatt agaacaatcc aaacagcttt tgaaactagg cattgaagcg aatgctttta
    13861 ttcgttatca attaaaagat tacaatactg gcttacactt acgatttttt agtggttcaa
    13921 tattatatca aaatagcact tttcgtccta ggtcttttcc aaattatggt tatacaatta
    13981 gtggaacgac tggcttaaat gactacaaat acgacaattt ctattttggc agaaacgtaa
    14041 ctgaaggttt agcatcaagg caaattagtg caaatgatgg ttttatgaag ttaacgacac
    14101 cagcaaataa aaattatcaa gaaggtagaa cattaaacta tttattagcg atgaatattg
    14161 ttttagattt tccaattcca catattccaa ttaagttatt tgcagatttt ggatatgctt
    14221 cagacaagaa aattcatacg atacaaaacc taccattcaa taaattttat tacgatgctg
    14281 gtttgatgtt tagctttttc aatagaggtt tagaatttta catacctttt attatgaata
    14341 aagaaagaga tacgcctaag cgtccaaaat tgggacaaag aatttctttt ttggttgata
    14401 tgcaaaaact tactttacac aaacaaatca gaaaaatgca attttaaaca atctattcta
    14461 tttgttgttt taaatttgtg tcgttcatat attctagaaa tagcaagatt tttaaaagta
    14521 ttttactcgc atcaggttta tgtggaatcg tttggggaat aattgctata atctatgcac
    14581 agaattggtt tgataatctt ggacatgctg aacttttcca agcaatgggt ttatatgaat
    14641 tggctttagg cattgcttat ttaatggctt ctaacaatcc attaagaaat tggcaaattg
    14701 tattaattgg tttcattata aaaatcacac taaccttagg ctatttatat tatagtttag
    14761 taaaagttga acctgatttt atattccaaa tgatactaat tactgatttg gtctggattt
    14821 taccatttgc gttggtgctt tataatgctt atatacacga ccaattatta gacaatgaaa
    14881 tgattggatt acaagctgca tcactgaaca gcattatgca tacaatggat actaataagc
    14941 aaaacaatct atataaacta tctgaacaac agcctgtttt gttggtattt ctaaggcatt
    15001 ttggttgctc attttgtaga gatacattaa tgagaattgg tgaattaaga aaaggtattg
    15061 aagataaaca aattaaaatt gtgttagtga atatgtatga taatgcaact gcagaagaca
    15121 atttaaaaga atataatttg gcagaccttg actatatagc cgatgaagaa tctattttat
    15181 ataaaacttt taagttgaga agagccactt tctcccaaat ttttggcatt aaagtattta
    15241 taaaaatgct caaaatattt tttataaaag gaacattttc atctttaaca gaacaagctg
    15301 atactttcca aatgcctggt atatttttag ttaagaatgg taatattatt caacaatata
    15361 attatacaag tattgctgat acaccaccaa tattagaaat tattgaagat gcggcattaa
    15421 ttcaaaccaa ttaaaattat tgaaatctag ggtgatgcat gatgagtgaa gtacgcaaat
    15481 attcttgatc caaatgtgta taaatttctg tggtagtaat agaagcatgt cctaacattt
    15541 gctgtactgc acgtaaatca gcaccacctt caataaggtg agttgcaaaa gaatgtcgaa
    15601 aagtatgcgg actaatattt ttttgcaaat ttatcttttc tgccaattgt ttaatgacaa
    15661 taaataccat aactctagtc agtggctttc ctcgtctgtt taaaaataaa aaatcttgat
    15721 actgctcttc gatattcaat ttatttcgga taaaatccat atatatttga atgtgcttta
    15781 tagcttcttg tccaattggc accaaacgtt ctttattgcc tttgccaatt acacgaataa
    15841 attctacatc aaaataaata tgagaaacac gaagattgac taactctgct actcttaatc
    15901 cacaactgta taaggtttca agcattgctt tatttcttat attttcatct ttggataaat
    15961 ctaaagctcc gattaattgt tctatttcat taaatgacaa agtatcaggt aatttcttgc
    16021 tcagttttgg agtttctaat aaaacacttg gatttttctc caaataatta tctaatacta
    16081 aatatttaaa aaaagacttt atgcctgaaa taatacgagc ttgacttcta tcgtttaaac
    16141 ctaaatcata taaaaatgca acaaaatttt ctaatagttt atagtcaata gcttcaattt
    16201 ttaattctgg atgatggata gatatatatt gttttaataa ttctacgtcg tgtagataag
    16261 cctcaataga attaatcgat aaggactttt cgattttcaa atgagcctga aaattcagaa
    16321 tataagattg ccaaagtaca ttgctatcca ttacattata gaaatccttg gaattttatc
    16381 taattcataa cattcaattt ctaatttctt tattgctcga attttttgtg ttgcaatttt
    16441 aaatttttca attgccacac ttttatttat tgcttccact tttaagacga tacttgacat
    16501 tctgatatct aaaacattat ctttataagc atcttcatca attttcattt gaattacaat
    16561 atactccttt gcataaataa aatcaaaaat tcttaaaata ttaaatggca taattattct
    16621 aattgattgt tttgtttaag ttgaagatat aaatttttat actttttgta taagaaatac
    16681 aaaacaatag ttattatagg atgaattatt acaaataata ttacatttaa ttgctgataa
    16741 gatatgccca ttaaatcact tattaatacc atcaatttta cacatatcca aaatattgta
    16801 tccatataaa ttaacttatt ttattttgag aaacttttaa aaaatattaa acaaagtaaa
    16861 aattctcaaa tatatagtca aatatagaat ataattacga atcatgaagg ccaattttag
    16921 ttgttgttaa gtcagttttg tatcgtttgt ataaattttc aaaactaatt ggtttttttg
    16981 ttgataccta tatatagatt gtaaactttt ttaggacgat attagtcgtg aaattctacc
    17041 attgccatcg ccaaaaggca aatgtttatt tgtagcaaat tactatgcca aagcctggag
    17101 catttaattt tttcagttct gtctttcaat tctactataa atatacactt ttttaattat
    17161 taaggtgaga accaatttca gaagaagaca ttatattttc ttaatatgaa agaaccacag
    17221 ccaaagctgt ggttctttat tctagagaat aaatattctt attgaaaaac tattaattta
    17281 cttttacagt aaatttaaac tcattaaatg cgggctctgt attggcataa acttttacag
    17341 tcttagattg atttccattt ctaccttcag agttaaaacc tatatctaaa gtatctgaag
    17401 ctccaggtgc taatggttct cttgaaaaag ttggtgtggt acaaccacag cttccaaaag
    17461 ccataaaaat cactaatggg ttattgcctg tatttgtata aactactttt ttattcaatt
    17521 tagtaccttc ctttacttcc ccaaaatcgt ataattcttt ttcaaatttt agagtcgttt
    17581 tatgttggtt caaactatcc attttagtag aatcaatagt ggtattgaat aaattatcta
    17641 cttcagatga cgcagtatta tttatttcat caggtttatt aatattacaa gctgtaacaa
    17701 atagagctgc aataattgta taaaaaacta cttttttaat ttgcatataa agtaaaaaag
    17761 attaagctaa taaactatca atttttttct ttaaatcacc tttagaagct aagcctacat
    17821 gtttacttac aacttctcca tttttaaaga ataataatgt tggaatactc ataatgccat
    17881 attgtgctgc aacatctcta ttgtcatcta catttaattt tccaatcaaa gctttccctt
    17941 caaattcccc cgctaattct tcaataacgg gaccaattaa tttacacgga ccgcaccatt
    18001 ccgcccaaaa atctaccaat gcaacacctt gtaaatctaa aacttcattc tgaaaattac
    18061 tgtctgttaa ttcttttgcc atgtctattt ttttaatgtt tgtatttaat aatgtaagaa
    18121 caaatatagt caaataatgt tccgaaaaaa taaaattatc gaattacgac ttcttttatg
    18181 acatttttgc ctagcttctc attaattatc tgacagattt tctcacgtgc cataaataat
    18241 tcttgcttca aaacagatga ctcaatcgtg atatataaat tgttttctct aaaaaagatg
    18301 tttttagtgt ttttagcaat tatgccttgc atttgttcaa accatatttt tttgatgttc
    18361 aattcgagca ttttatctcc aattttcatc tctcgaatca tttcatcgaa gatatctttt
    18421 agtggactaa attgatgctt tgccaaaatt taattaataa gtatgtgttt aaaatctttt
    18481 atagataact tttgcaagat acttaaaatt cggttgacat cggtataagt aataaatatt
    18541 tgtccaaaat tttcttcatg aattatttga agaatttttt gaatacgaac catatctaat
    18601 ttatcaaaaa tatcatctag aaataaaagt ggaagcttct tagtgcatct tgcaatatat
    18661 tcatattgtg caaatttaag tgccaaaaca atagtttttt gttgtccttg tgatgcaatt
    18721 ttttttgctg gaatattatt catcataaat gaaatatcat cgttatgaat gcctattaat
    18781 gttcgttgtg ctaatatttc atcttgtttg ttttgattga ttaatgtatc aatattttga
    18841 cctttgaatt tgctttcata ttctaattga atatgctcat cgccatcaaa aatctgcttg
    18901 tatttagttt taaaaatggt gttgaattgt gtaataaatt ctattctttc cttgaaaata
    18961 tatgtgttta agtcaattaa ttttgtattg taaatatcta atacggtttc acttgaattg
    19021 ctgaatggca attgttttaa tagagcattt ttttgtttta taagtttttg atagtctata
    19081 agtttttgta aataaattgg attacctaag gctaatgtag catctaaaaa ttttcgtcta
    19141 ttttcagcac ttccaataat taaatcttga tcataaggtt caataaacac aataggaaat
    19201 cggccactga tttcaatttg cttttcaatg ggaatattat tcagccaagc ttgtttttta
    19261 gaatctttta catttagcac tttgattgaa aaattattat cttgaatttg aatatttgtg
    19321 tttaattgaa ttttgtcttt ttcaaaaggg aaaacaaatt ggtcattttt gaaaaaataa
    19381 ctttttagaa aacaattgta ataaatagca tctagtaaat tggtttttcc taaaccgttg
    19441 tttcctgtga tgatatttaa tttgtgatga aattcaaagc tttgttgtag atattgttta
    19501 aaatataaca attcaatatg tgatacaatt tgagcaggga agttcatatt tggaacaaat
    19561 taattaaaaa aattcttctt ttgctaaatt aattgtattt ttgcgattct aaaaattagg
    19621 ttgaatggca aagaagcaac aagaaagtca agcacttcag aaagtaaatg attttatgga
    19681 taatatcgat ttatttatcg aacaatataa aaatattatt atcggtgtta ttgttggttt
    19741 agttttactt tttggtggat tatatgctta caaacatttt ataaaagcac ctaaagaagc
    19801 aaaagcaaaa gatgcgattt ttaatgctca atatttattt gctaaagatt ctttccaatt
    19861 agcattaaat ggttctggaa atatccaagg atttaaaaaa attgctgatg aatatggcag
    19921 cacggcagct ggaaatactg caaagtttta tgctggtgtt tgtaatttga atttaaaaaa
    19981 atatgctgaa gccgttaagt atttagaaga ttttagtacg aaagaccctt tggtgaatgc
    20041 tagaaaatat ggctgtttag gtgatgctta tgcagaacaa aaaaagatgg atgaagctat
    20101 caataattac aaaaaagctt atgcttatga taatgaagtt acggcaccaa cttatatgta
    20161 tagagccgca ttggcattgg aattatcaga taagaaaaat gaagctttag aactctataa
    20221 aaaattaaat acaacttatc caaattcaca agaaggacaa gctgctgaat tatccattgg
    20281 aaaattggaa caacaagtaa aatagactta tagtgtcaag atttctctga tttaatgata
    20341 catataataa atgcgttttg tactgaaata actattgact aacaactatt gactaataac
    20401 taataaaagt ggcatcagta ttacaaaatt tatcaaacta cgatgaaaaa agtgttcctt
    20461 caggaaaggg caaaaaaatt ggtatagctg tcgcagaatg gaataaacat attactttta
    20521 gtttattatc aggttgtaca gatactttaa caaagtttgg tgttaatgaa gaagatattg
    20581 aaatttctat tgtaccagga acttttgaat taccgtatgc tgcaaaacaa ttgtatgaca
    20641 aaaatatgaa tgcggttatt tgtataggat gtgtgattaa aggtgaaacc gaccacgatg
    20701 tttatatcaa ctattctgta gcgaatgctt tacaaaattt gaatattcaa tacaatattc
    20761 cgtttatttt tggtgtttta acacctaata atgaacaaca agcattggat agagcaggag
    20821 ggaagcacgg aaataaaggt actgaagctg caatcactgc attaaaaatg atgcaggtat
    20881 aatatttaga aaaaattact attttagcaa aatgaaattt aataataata attggtattg
    20941 gcgtaaatca taacttcagt gtgatgcaat tttcaataca atacgaattt atatagattg
    21001 acttgcttca cgcaagtcat ttttaattta agctcaataa aataacaata tgtcaaaata
    21061 tacttttaaa actagctcca aaaagattat cgcagatatg cttacgccag tatctatata
    21121 cttacgtttg cgtgaacaat ttaaaaatac agttttatta gaaagttctg attatcatgg
    21181 cgatgaacat aatttttcct atatctgttt tggtgcaatt gctggttatc gattagagaa
    21241 agatgttttt tcatcgtttt atcccgatgg tcatacagaa aatattcaaa ccaattcaca
    21301 aatgcatttt ggtattttgg taaatcaatt tatcaaacaa tttgaaactc caagtacaaa
    21361 tatttctgat aaaattatga ccaatgcctt atttggttat atgacctatg atgtaattga
    21421 gcaatttgaa gatattcaat taagacacga aaaacccaat attccaaccg ctcaatatta
    21481 tgcatatcaa tttataatag catttaatca tttcaatcat gaattgtata tcataaaaaa
    21541 tgctatagaa ggtattgaat ctaaatataa tttttcttta gatgcaattg aaacgatgat
    21601 taaaaataat gaatttaatt tgggacactt ttcatcaata ggcgaaaaaa cttcaaatgt
    21661 tgataatgaa acgtatattg aaatggtcaa acgtggaatt gcccattgtt atcgaggaga
    21721 tatttttcaa attgtgttgt ctcgaagatt tcaaaaggaa tatgaaggtg atgattttaa
    21781 cttgtaccga gcattgagaa gtatcaatcc atcgccatat tgtttctatt tcaattttga
    21841 agattttaga ttaattgggt cttcgccaga agctcaattg attattaaaa acaataaagt
    21901 aacgatacac ccaattgctg gaactttcaa acgtactggt aatgatgttc aagatgcgaa
    21961 tttagcaaag caattatcgg aagatgagaa agaaaatgcc gagcatataa tgttggtaga
    22021 tttagcacga aatgatttgt ctagaaatgg gaaaaatacc gttgttgaag tttataaaga
    22081 aatacaatac tattcacatg taattcactt agtatctaaa gtatcaagtt ttattgatga
    22141 tacagcagat aaaataaaaa tatttgggga tacatttcct gctggaactt tgagtggtgc
    22201 tccaaaatac aaggctatgc aattgattga taaaatcgaa aatacaccga gaagttttta
    22261 tggtggagca atcggctgtt taggttttaa tggagatttt aatcatgcga ttatgattag
    22321 aaccttttta agtaaagata atttattgac tttccaagct ggagcaggag tggttgcaga
    22381 ttcaaagcca gagagtgaat tacaagaggt ggaaaataaa ttaaatgcct taaacaaagc
    22441 tatagattta gccaatacaa tttaatagat tgccttattt ggtatgtgtt tgttgcatta
    22501 aatagtacat caatttaaaa acttcttagt gatactcaaa atttccttta tctgaaataa
    22561 tactaacttt tttagaatca gcaggttcta ctcgcccaat gatttgtgca tcaatattaa
    22621 aactttttga tatagcaatg acctcattag caaattgttc attgcaataa atttccattc
    22681 ttgaaccaca attaaaaact tgatacattt cttgccagtt ggtattgctt tcagattgta
    22741 ttaaattgaa taaaggagga actgcaatta aattatcttt gatgatatga aggttttcaa
    22801 tgtaatgcaa aactttagtt tgggcaccgc cactgcaatg tatcaaacca ttgatgtttt
    22861 ttgttccaat agtttggatt atttttttaa taacaggtgc ataagttcga gtaggagaga
    22921 ggactaattt tccagcatct ataggtgttc cttctatttt gtcagttact aattttgtac
    22981 cttgatatat aaattcagaa gcggtattgg gatcaaaaca ttccgtgtat ttttgagcat
    23041 aaatatttga aaaaacatcg tgtcttgctg aagttaaacc attactgccc attcctccat
    23101 tatattcttt ttcgtaagta gcttgcccat aagaagcaag tccaataata acattaccag
    23161 ctttgatatt tgcattatca atcacatcat ttctgtgcat tcttgcggtc ataacagcat
    23221 ctaccgaaac agttctaatg acatcgccca tatccgcagt ttctccgccc attggaaaaa
    23281 cagtaacgcc atgttcattt agcatttcaa cgacttcatt cgttccgtca atgatggttt
    23341 tgattacttc tccagttatt aagtgtttat tacgattaat tatagatgaa agtaaaaaat
    23401 ttgaagttgc acctatacaa atcaagtcat ctgtattcat gacaatagca tcttgtgcaa
    23461 tgcctttcca aaccgaaata tcgcctgttt ctttccaata gatataagct aaaactgatt
    23521 ttgttccagc accatcagca tgaatgatag agcaaaattg ctcatcatta gtcaaataat
    23581 caggataaat tttacaaaag gcttgtggaa ataatccctt attttcattt tttatagcgg
    23641 cgtgtacttc atcttttgta gcagaaacac cacgctgatt atatttttca gaaatcattg
    23701 tatcttattt agaaaatcta ttgtagtctt acaaaggtaa ttaatttaaa ctacttcatt
    23761 tgattaaacg ccaaattgtt tgaggtgatg atctaagtgt ttatacatcg cacgtcccca
    23821 agtatcttta tccatttctc caaaaatagg atgtgcgcca gttaagttct tagcttcaat
    23881 aaattgtttg taaacttcca aaaatgattg tttggcttgc tcaaattctg gatctgtttt
    23941 aacaataaaa tctttagcag tcatcatatt tttaggccat ggaataatat caattactaa
    24001 ttttcgcatc aagggattgg caaccaaaaa ttgtaaattt tccttaggta agctgacttt
    24061 attaatcgga acagcaaaag ctatttctaa atgttttagc atttgagcaa cagtcatcgt
    24121 tccccatgct ctttttgagt tcgcatttag attttgtatt ctctgagcaa tggcttgttg
    24181 agtacttttt tcaaataaag attccatatt attttgttat tgcaaaaatt agtatcatga
    24241 tttattacct tgttttctta cgattttttc aaattactga tcatttacct tcttccaaat
    24301 catcatttgg ttagagtaaa ttttcgcaaa accttttaca tcatcgccag accaagaatt
    24361 gttcatttcg ccataacttc caaatttgct actcattaaa tcgtgttttg attcaatgcc
    24421 aataaggtta aatcgataag gcaataactg cacaaatact tttcctgaaa cacatttttg
    24481 agtactgctc aacatcgctt caatatctcg cattaatggt tcatagaata atccttcatg
    24541 taaattactt ccataaactt gtgcaatttg gtctttccaa aacaactgtt gtttggttaa
    24601 agtatgtttt tcaagtaagt gatgtgcctt aattaaaatc aaaggagctg ctgcttcaaa
    24661 cccaactctc ccttttattc caatgatagt gtcgccaacg tgtacatctc ttccaattgc
    24721 ataaggagca gcaattttat tcaactctat gattaattct gttgggtgat tataagcttt
    24781 tccgttcaaa gcaataggtt ctccttgctt aaattcaatc tctatatcta ttggcgtatt
    24841 ttcttctaat tgagtaggat atgcactttc aggtaaagta tttcttgatg ttagggtttc
    24901 cttcccacca acactcgttc cccaaatacc ttggttgata gaataagcag cttttttagc
    24961 atcgatttct atgccatgct gttttaaata ctcaatttcc tcttctcttg ataatttatt
    25021 gtctctaata ggtgtaatga tttttgcttc aggcataaca atattaaaaa tcatatcgaa
    25081 tcgaacttgg tcatttccag cacctgtact tccgtgtgca atataacctg caccaatttt
    25141 tttagcataa tcagctacag ccatagcctg aaaaactcgt tctgccgaaa ctgaaagtgg
    25201 gtatgtattg tttttaagca cattgccaaa taataaatat tttatgcaat tgtcataata
    25261 attttgaata atatcagcga ctacatattc tttgacaccc atatattttg ctcgttcttc
    25321 aatgcctttt aactcatcaa cactaaaacc tccagtattt acagtaatgg catatacatc
    25381 taattttaaa tcttgggata aatattttac acaataagag gtgtctaatc ctccgctata
    25441 ggctaatact actttgttgt tgctcatctt tatattttat ttaattacat taattagaat
    25501 tgtctttatc ttctgttttt ttcttatttg ccttgcttct tccagcagtt tttacttctt
    25561 taattctttg tgcctttggc gattttagtt tttgaggtat ttttttatca acttttttaa
    25621 tagttgcgac aaattctttt ttcttacttg cttttgggtc aaataacata gcagtacata
    25681 aacagtttgt tctacctgtt cttgttaaaa catcataata ttgacaactc atacagccat
    25741 cccaaaacgc actatctctc gttaattcag aataagttac aggtttatat cctaaatctg
    25801 agttcatttt catcacggct aagcttgttg taatgccaaa aaccttagca tttggatatt
    25861 tttttctagt atattcaaaa acaaattttt tgattttttt tgctaaaccg ttttttctat
    25921 actcttcttt aacgattaaa ccagagttag caacataatt tttaccagtc caagtttcaa
    25981 tatagcaaaa tcctacaata atttcttcac caccttcgtg tgttaaagca atgatagcct
    26041 tttgttcttt tattttagct tctaaatatt ctggcttcct ttttgaaatt cctacacctc
    26101 ttttttttgc agattcttcg taaagtttgc ataattcatg cacatatttt gcgtgttgct
    26161 cctctgcaac aataattgaa aatttaggtt taagtgcttt caattttaat agaattagtt
    26221 ctaacaaaaa taagtgtagt aatttaaaat caatgttatt tatttaaact tattttgcac
    26281 taataatact aattgttttt ttacatttgt ctaaaatttt agtatgacct tatttagttt
    26341 gtttgttttt gattggtatg caaaaccaga aatatttatg ataaccgata attttggtat
    26401 tagatggtat agtttgtttt atggtatggc attttttatt ggatactata ttttaagctg
    26461 gatgtttaaa aaagaaaaca aaagtattga agatgccgat tctttattga tgtatatgtt
    26521 ttttgcagta ttgattggtg caagattagg acatgtattt ttttatgatt ggccatatta
    26581 tagtcagcat ttgtcagaaa ttcctcaaat ttggaaagga ggtttagcta gtcatggagc
    26641 tgcaattgcg ataccgatag ctttatattt atataaaaga aaacatccag atcaaaccta
    26701 tttatggata attgatcgaa ttgctgttcc attagcatta ggctcttctt ttattcgttt
    26761 tggaaatttc tttaatcaag aaattgtggg taattatact gatggatctt ggggtgtatt
    26821 ttttcataga catatagatg aattaacagg catgttggac acaggaccaa gagttccagt
    26881 tcaattgttt gaaggctttt tatatgtgtt tttatttatt gttgttctga cttattatat
    26941 tgctaaaaaa tacaaaccta aagaaggctc agtgatggct atttttctat ttataatgtt
    27001 tataggtcga ttctttttag aattttggaa agatggcgaa aagatagttc atttaggaag
    27061 cattgaatta aatagaggta atgtattgag tattccattt attataatag gattcattgt
    27121 cttttttatt tcaacaagaa agaaagacgc cgtaaaaatt taaacaaaat tgtagcaaaa
    27181 aattagttaa tttttattgg acgaatttcg ttgatttgct tatctttatg caataagttt
    27241 aatcatttat gataaagaaa gtttttgcta ttgttttttg tatattactt agttacacag
    27301 tatttgcaca agctgaggtt attaatcgtt cagctgctga ttctttatta gctccgtttt
    27361 atcatggtgt ggcatctgga gacccaacac ccaattcagt tattatttgg acaagattaa
    27421 ccacttatga tgaaggtatt ttgagtggta cttggaaagt agcaacggat acgaatatgc
    27481 taaatgtaat acaacaaggt gtttattcaa cggatagctc aaaagattat actgtaaaaa
    27541 ttgatgtggg tggtttggag cctggtactt ggtattttta tgaatttgaa tataaagata
    27601 gattttcttt gagaggaaga acaaagactg caccggttgg tggtgtcgag caattacgtt
    27661 ttgcatttgc ttcttgtacg aattatcctg ctggatattt taatgtatat gatagaattt
    27721 tggaaagaaa cgatattgat gctgttttat ttttaggtga ttatatttat gaatatggtc
    27781 ctaatcagta tggctcaaca cggaaagatg ttatgccaac taaagaaatc gtaaaactag
    27841 aagattatag attaagatat tctacttata gattagaccc agcattgaga aaactacacc
    27901 aacaatttcc attttttaca gtttgggacg atcatgaagt ggcaaataat tcctataaag
    27961 atggtgctga aaatcataca cctggtgtag aaggaaatta ttatgtaaga aaatcagcag
    28021 cacaaatcgc ctatagtgaa tggttgccga ttcgattgcc agaagaaagt aatccaagta
    28081 aaatttggag aagttataaa tatggagact tagctgaaat ttttatgtta gatactcgtt
    28141 tgtatgatag aagctatgag aagatgaata aaaatgatac tacaaagaag cttttaggaa
    28201 acgaacaaat ggagtggctg aaaaatggat taattaattc aactgcaaaa tggaaaattt
    28261 tagctcaaca agtgatgatg actaatttag atccttttgg actggtttta aatggcgacc
    28321 aatgggatgg ctatccttta gagagaaaaa aattatatga tattatatta gacaatgata
    28381 ttaaaaatgt aattacctta actggtgata ttcatacagc ttgggcaatg gatttgcctt
    28441 atgataaaac aaaatatgat ccaaaaactg ggaaaggctc tgtcggtgtg gagtttgtat
    28501 gtacgagtgt aacttctcca agtgtgccat tcccgctaga cccattgtat ccaattgtct
    28561 atggcgtttt accacatatt aaatatactg atttacactg gaaaggtttt agtattttag
    28621 atttaaaaac gactcaggct caaggcgatt tttatacagt ttctagaatt gataaacttg
    28681 atgctggtaa aaaatatgta acaggttact atactttgga caatacacgt tggttgaaaa
    28741 aagcgaatca agaaacgaat aaaatagttc caaatatcta tttttctcct ttttatccaa
    28801 gattggcaag tcaaacacct ataaagaata atctaaaaag tggtgtaata ataggtgttt
    28861 atccaaatcc ttttattcaa gatattcaaa tgcagtttaa tttgtttcat accaatccag
    28921 ttatgataca aatagttgat atgaatggca aaatggtttt ccaaaaagac atgggaacac
    28981 tgaataatgg tttgcacaat gaaaagataa cagttgaaaa tattccagca ggcgtttatc
    29041 aattagtaat tagaagtgga aatgatataa ttgccaaaag catacagaag ttttaaatat
    29101 aatagtcgtg aaaaaaacaa aagctagtgc atctttatct gcaaatgcag ataaaactaa
    29161 ggttaaaaag gtagctcctg aaaagaaaat aattgatact aaaatatatc caatcggaat
    29221 atttgaattt aatgaacaaa tagcaaagga aaatatagaa acctatattg aagaaataaa
    29281 ggaattgcct aagaatttaa aaaaagttct aaaacaggtt actaaacaca ataaagaaaa
    29341 ttcttatagg gacgaaggtt ggacgattga acaaattgta catcatttgg cagatagtca
    29401 tattaatgct tttattcggt ttaaattagc attgacagaa tattttccta caataaaacc
    29461 ttataatcaa gatttgtttg ccgaaacttc agatgtattc ttgtcgagcc tgaaatcttc
    29521 ttatcatata attgatggaa tacataaaag atggacgatt ttattggaaa atatggataa
    29581 tgaagatttc aaattgggat atattcatcc agaggacaat caaaaacatt ttttgttaaa
    29641 agtgttagca atgtacgctt ggcatggcaa acatcatatt gaacaaataa aagtagcctt
    29701 aggttctgat tctgaagatt aagacattca taacttttca gtgtttctaa ggagttattt
    29761 tttattatct ttgctacctc attattataa aatatggata aaaatacgat tactggtatt
    29821 gttttaatta ttttgatttg ggttgctatg agtgtttaca actccaagca acaagagaaa
    29881 tttgaaacta cacaaaagat agaacaaaaa tcagagaaga aagatacctc agttgcgagt
    29941 ttaccaaata ttgattcagt tcaaaaaaat gcagttgata gttcagttga taataaatta
    30001 gatttagtag aatatggtgt ttttggagca gctgcaaatg gaacaaaaca agaaattata
    30061 gttgaaaatg aagattgtat ttttactttt tccaataaag gtggcgtatt aactaaaatt
    30121 gaacttaaaa aattcaaaac ttatgaccat aaaccattaa tcttatttaa tcaaacgcct
    30181 gaagatttca attttacctt tgtagttaat agtgctaaaa taccaattca aacttctcaa
    30241 ttatttttta ctccaaataa tggaaacaaa gtcataaaag gtaacgaaat ataccaatta
    30301 gattatacta tttcagatat ttcaggtaag ttatatagtc agtcatatac aatttctgga
    30361 aaaggatatg ttgtaggttt tgttgctgat gccaaaggat tgtctaaaga aattccacaa
    30421 agtgccaata ttattccatt agcttggagt caagaaatac aaagccatga attaaatcat
    30481 aaggaagaaa aaatgagtag tggtgtatat tatcaagata tagataaaaa tgttgatgat
    30541 attggtttaa tgaaagataa agatgaaaaa attgaaaaag ctattaattg ggtttcactt
    30601 tctcaaaaat ttttcaatac aacattaatc gcaaaaaatc aaccatttct tgccaattca
    30661 acagtgaaaa caattgctgg aacagatgat tcttctgctt tagtaaagaa attatttgta
    30721 gagttaaaag taccattcaa taatgaaact aactaccagt ttgatggtaa gttttatgct
    30781 ggaccaagta gttttaaaga attaaaaaaa gttgaaagtg gattacaaca aatcattcca
    30841 gttggtggaa gtattttggg ttgggttaat aaattcctag taattccaat ttttaatttc
    30901 ttaggtaaat ttatttctaa ttatggtatt attattttaa ttcttacttt aataattaaa
    30961 ttaattacgc ttccatttac ttataaatct caattatcta tgataaaaat gagagttcta
    31021 aaaccagaaa tggatgcatt gaaagaaaaa tatggaaaag accaagcaac ttttggagct
    31081 aaacaaatgg agttatatca acaaactgga gtgagtccgt ttggtggttg tattccaatg
    31141 ttattacagt ggccattttt aattgcaatg tatagatttt tcccatctgc aatagaatta
    31201 agacaagaga aatttttatg ggcaaacgat ttatcaactt atgatgcagt aatttattta
    31261 ccattcaata ttccaatttt aggaagtcat ttaagtttat ttgcaatttt aggcgttgta
    31321 acatcatttg gaatgacttt atatactatg aaaatgcaac cacaacaaac aagtggcggt
    31381 ggtgaatttg ctgaagcaat gcaaaagcaa atgaaagtaa tgcaatattt ctttccattt
    31441 atgattttat tcttctttaa tagttcttca gctgcattga gttactattt cttcttgtac
    31501 aatggatttt cgttgttaca acaatggata atgagtaaat tctttattga tgaaaatgct
    31561 atcagagcac aaatagaaca caataagaaa aatcctaaaa agaaatcaaa cttccaaact
    31621 agaatggaag aaatgatgaa acaacaacaa gctttaaagg aacaacaaaa gaaaaaataa
    31681 actaaaattt atttattttt tctaattatt gtatatttat attaaaatat atcaatatat
    31741 gaatgttaga aatttactct tagttttatt aagtattata ttttttagta cgaatgttat
    31801 tgcaaaatca gatgtaaata ttaatatagc actatactat attaagaata attataaaga
    31861 aattggtctt gaagaattgg atattaaaga cttaaaacta aaagatattt actttgatga
    31921 tttctcgtct ataaatcgaa tttggttcca acaatacaat aattatcttc cacttaaaaa
    31981 tggttttata ggcgttcata taaaagatgg acaagttgta caagttacca ataatggaaa
    32041 agcaaaccta aaattaaata gtcagaatct tattgtttca attgacaata aaactgcaat
    32101 actttctgca attgccaaag ttttaccaaa gtcagaatta gaaaatttaa aacaaacttc
    32161 aagtgataaa agagtacaaa aatatgctta tacagcaaat gatgtagaag ctattgatga
    32221 taatattgat acttatttgt cttatgtact tgatgaaaat aataaaattg atttagcatg
    32281 gatagtaaat tttggtacaa atcataaaaa ttactgggaa attgaagtga gtgccgttag
    32341 tggcaaagtt attagaattc aagatagagt tttgcattgt agctttggga aaggaggatt
    32401 tttagaatca attagcgacg aggaaattca ttctgaggag atacataatc attcaaatag
    32461 caatcaaaga acggaaggag ctccaagtgc tgcttatcga gtttttcctt ttagtaaaga
    32521 atctccaatt tatacaccac gtttattgat taaaaatcca tcagatgata ttgcttctcc
    32581 ttttggttgg cacgatgcaa atgggcaaga cggtatagaa tttacaataa cgagaggaaa
    32641 taatgtatat gcttatgctg acaaagcagg taataataaa gttacaccag gttctccacc
    32701 tgatggtgga gcaaatttga attttgattt tcctttagat tttagtaaaa gattagatac
    32761 attagataat tcaaaagcag ttattactca attgttttat tccaataatg taatacatga
    32821 tattttgttt aataatgggt tcacagatgc aaatagaaat tttcaggcaa aaaattatgc
    32881 atctggaaat actgctactg aaaaagattt tatatgggca gaagctcatg atggtgcggg
    32941 agaaaataat gcaagttttt atagtgctgt tgatggaagt aattctccag tagcaggagg
    33001 tcgctcaaga atgcagatgt atatgtggtc agatatttca aattataatt taatattatc
    33061 tcgaccaagc ggtattattg atacattgag atgtggtact caatcagatt ggggcgtttg
    33121 taattttgat atcactggag ctgtgaaaaa tgctttttct ggttcagtta gcttaccaag
    33181 ttatgtttgt tcagcagtaa gcaatagtgc agaaattttg gaaaaatagc tataatagac
    33241 agaggtgatt gtgatttttc tgcaaaagta tataatgctc aacaagctgg tgcgattgct
    33301 gcaattgtag tgaatagaca atctgcaggt gatactatta ttaatatggc tgctggtaca
    33361 tctgctaatt tggttattat accagcattt tttgttactt atgcagatgg gcaaaagata
    33421 agacaatata ttgatagtgg gaatgtaatt cggatgaata aaacgtcact aggtaactgt
    33481 ctaagaaaag atggctcatt agaaaatgct tttgttgcac atgagtatgc tcatggactt
    33541 tcaatccgat tgactggaac tggtgttaat tgtttaaaca acaaagaaca aggaggtgaa
    33601 ggttggagtg attttattgc tttagctttg acggtaaaac catcagataa taaaaataca
    33661 gctcgaggtg ttggtaatta tgttgttagt gaaccaataa atgggaaagg cataagaatg
    33721 tacccatact cttatgatat gagtataaat ccattaactt atggttcttt aatggatatg
    33781 gatggcacac caattcttga ttcggaaggc gaatttattg ggtatgtacc taatagtgta
    33841 gaagtgcatg atatgggtga agtttgggct gttactttat gggatatgta ttggaatttt
    33901 atagaagctt atggatttaa cccagatttt tataatggca atagtggaaa taataaagcc
    33961 attaaattag taattgaagg cttgaaatta caaaaatgta gccctggatt tttagatgct
    34021 cgaaatgcaa tactaaaagc ggattcaatt atgaatggtt atgctaataa atgtttgatt
    34081 tggcaatctt ttgctcgaag aggaatgggt tggtctgcaa ttcaaggttc atctgatcat
    34141 gcaaaagacc aaacagaagc gtttgatatg ccaccatctt gtaattcaaa tccaattcca
    34201 aatttcacat taagtgatac ttctatttgc ttaggagaat taatacaatt aacaaataca
    34261 acaacaggta atattgattc attaagatgg aaaatgatag gaggaagccc tgttagttca
    34321 aatgaaacta caagtctttc tgtaaattat ccatctgctg gagaatattt tataacctta
    34381 tatatataca agaatagtta tatagactct ataactaaaa aagtaaaagt atataatatc
    34441 ccagttatta ctactaatat ttcttcctca acaatttgcc caggaagttc tgtaacattg
    34501 tcagcatctg gtgctagcac atataactgg agtaatggag caaataatgc tcaaatatct
    34561 gtaagtccta ctgtaaatac ggtttacaag gtggtaggta aatcaaatgg atgtatatct
    34621 gattctgtag ctgtgtccat attcttaaaa actaaaccaa cggttagtat aaatgcgtca
    34681 tctttatcta tctgtgttgg tgaaactgat acattaacag caagtggtgc aacaagctat
    34741 atttggagag atggttctac aagtaatagt attgtggtca atccaacagt tacgagttct
    34801 tataaagtaa taggaatatt aaatggttgt tcttcagatt cagttgaaat acaaataact
    34861 gtaaaaccta aacctattgt aagtattagc cctacaaata caacaatttg tgctggacaa
    34921 tctgtaacac ttacagcaag tggagccagt agttatgttt ggagtacagg acaaagtgga
    34981 aatactatag ttgtaaatcc ttcagtaaat tctacttata aagtattagg aattacaaat
    35041 aactgttcat cagattcagc tactgctata gtaaacatta caacaacaaa tccaacggta
    35101 agtattacaa aatctccgaa tgtaagtaca atttgtgagg gcgattctgt aa
//
LOCUS       DAOQCW010000010        34384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_444_length_34384_cov_5.635002, whole genome shotgun sequence.
ACCESSION   DAOQCW010000010 DAOQCW010000000
VERSION     DAOQCW010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 34384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34384
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_444_length_34384_cov_5.635002"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1349)
                     /locus_tag="PLS16_01835"
     CDS             complement(<1..1349)
                     /locus_tag="PLS16_01835"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNJ59846.1"
                     /translation="MNKLTKNLLGLMMLLVSFVAYAQTVISGKVTDEAKEPVIGANIS
                     IKGSSEGTITDIDGNFELTTEQSTPFTIVVSMVGMENVEKEITGSTSGLDIGMKEEHN
                     ILNTVVVSASRVEEKILESPVTIEKMDQKMVKQSSSNDYYDDLSKLKGVQVINGSMNL
                     TSVNTRGFGGISNTRFVQLMDGMDNAAPLLNFPTGNIVGIGELDVQNVELVPGAASAL
                     YGPNAFNGILLMNSKNPFDSPGFSAQVKGGFASASNGYGTKPLGSVAMRVGHVWKRKG
                     QDYVAIKFNANAFQGTDWYANDMSTRDDNGPLGAPNYDGMNVYGDETPIQVPYGGIRG
                     NLIASTSGSLQNNPAFVGAVTAGVTAQVTAAVTQQVNANRPAIEAGVTAVVRQNAYNQ
                     AYAAAVAASDPTPEATANAQADGYISSPAGIATIQQQTNIKIQQLIQQNVAAVLPGQ"
     gene            1559..3412
                     /locus_tag="PLS16_01840"
     CDS             1559..3412
                     /locus_tag="PLS16_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNJ59847.1"
                     /translation="MNYLSVQNLSKYYGERLLFKDITFFIDKGQKIALIAKNGSGKSS
                     LIKILQGIEPPESGEIKFHKDIKVGFLIQDPIFDKNQTVYEAILNTDNELLKTCLNYE
                     QALAGNGNLNDAMEQMEKLQAWDYEQQIKQILSRFLIHDLEQKVNTFSGGQIKRLALA
                     ILLLQQPDLLILDEPTNHLDLDMIEWLEGYLSKDNITVLMITHDRYFLENICTDILEM
                     EDNILYKHKGNYSRYLENKEIREEHQRVNVEKAQNIYKKELEWMRRQPKARTTKAKSR
                     EDNFENVKKAATQKAQEKKVEIEIKMNRLGGKIVELHNISKKYGQKVLLDKFSYKFQK
                     GERVGIVGRNGCGKSTLLNIIIGNDKPDEGNIVLGETIVFGYYNQNGMHLKEDKRVIE
                     VIKDIAEYIPVAGGQLSAAQLLERFLFAPEQQYNYVSTLSGGERRRLYLLTILIKNPN
                     FLILDEPTNDLDILTLNVLEEFLLDYKGCLIIVSHDRYFLDKLTDWLFVFEGNGKIKT
                     FLGNYKEYRDDLKEQLRLEKGNQTKIVSNNSKSTNEVPKKKLNFKEQQEFIQLDKDIS
                     TLSEEKDTLTAQLSSGAIDHNEIQNISSRLQEIIDLLDEKEMRWLELSEFA"
     gene            3461..4078
                     /locus_tag="PLS16_01845"
     CDS             3461..4078
                     /locus_tag="PLS16_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59848.1"
                     /translation="MQKRLSHNYYCLLLNIIVFALCLTTLNSNANDLNKPFLSFELLP
                     TAFKKQLNIVNSEFTFAYGMRQDSVMRDIRNYTKEDIETFIHSRKDYEYIFRIPFWAA
                     MDSIRYSWLIPKLINDLSDTTFVGLINASDVTIWSRVKTGDMVHNTLNYQIDDDIFTV
                     AGRASWILKRLSKNEYGIIKPNMPSKEIKEIINRWNKWYNTLIRK"
     gene            4195..7302
                     /locus_tag="PLS16_01850"
     CDS             4195..7302
                     /locus_tag="PLS16_01850"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HNJ59849.1"
                     /translation="MKLKQYKIINTLLGLIVFAISLWQYASTAESAGSLWDCGEFISC
                     AYKLQIAHPPGAPLFLMMGRVFSLFASDPSHVAFMINLLSAVMSAGAVMFLFWIITII
                     AKRIVARNEEDLTTDRVISIMGSGLVGALACSFSDSVWFSAVEGEVYAASLFFISITF
                     WAVMKWDEEADEPHANRWLVFASFMIGLSIGVHLLSLLVIPAAALIYYFRKYEPNIKG
                     FIIAFIVGFFVLGLVQVGVVSTAPLIASKFEYFAVNILRLPFNSGLIFAIILFFGILV
                     AIIRYANKIKHADMQLSAICALVIFIGFSSYAMVPIRATAFPPINMNNPSDAYSLISY
                     LNREQYGERPLLKGPMFDAQPYAIDEKGEVLYPNKETKKYEVKDIKRAYKYKDEEMLF
                     FPRMGPTNDGANAKQLFQYWTDFQGTPTFSDNISYFLSYQVGYMYWRYFMWNFAGRQD
                     DYQGMAGTQKINGDWLSGLSFIDNSRLGSQENLPYQITNQKARNKFYLLPLILGLIGL
                     VYMVNRSKEYALVLLLLFLNTGLFLIIYINEPPREPRERDYALVGSFYTFCIWIGFAV
                     LAVAEWIREKSKGLALPVSIAVSLLFLGVPYIMGKEGWNDHNRHNRFMARDFASNYLE
                     SCPPNAILFTQGDNDTYPLWYAQEVEGIRTDVRIVNLSLLGVDWYIDGLFRAENKSTP
                     IPLQKDFTPDKYRGSTRQVLSYNPQSGFADPNQYYDLSNILSFVLSDDMKYKAQTQGG
                     ETVNYFPSTKFKLKVNKEAALKNNVVPDKYKDLITDEIVWDLGKDRVIIYDLAVLAMI
                     AAEDWSRPICFANTVESSYYQGLDKYMIQEGLINRIVPVKFEENQRGFMAMNTDKNFD
                     LVQNKFKYGGLGEREMFVDENSARIMNSQKSVHLSIADDLIRNGKNDEAIKVLDKAKK
                     EYQYVNAPYYAPNNRFFNILAIQWIDLYYRAGKPEGAKEIKELFLKDMADCLRFYLLP
                     NNSYAERYADEKKSAEDMIQRMQYLAINYKDNDFMKQLNQAFPNYVQSPTIEPNMVAP
                     QQIFR"
     gene            7330..7708
                     /gene="mscL"
                     /locus_tag="PLS16_01855"
                     /pseudo
     CDS             7330..7708
                     /gene="mscL"
                     /locus_tag="PLS16_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587729.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
     gene            complement(7760..8407)
                     /locus_tag="PLS16_01860"
     CDS             complement(7760..8407)
                     /locus_tag="PLS16_01860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ59850.1"
                     /translation="MSAFIPKFSIVLTTYNREQYIERAILSVIQQTESNWELLVIDDG
                     STDNTEKLIQPYLNINQSVYYFKQENRGEVSAKNRGISLSCGEYITFLDSDDAYKMEH
                     LQYRSQIINQHPMVDLLYGGVEVIGDEFVPDRRNKGRRIHLSDCVIGGTFFIKREVLF
                     ELGGFRTIDIGADADLFDRIVRAKKNILKMNSPTYIYHREYEDSITHNYFQERKH"
     gene            complement(8451..10688)
                     /locus_tag="PLS16_01865"
     CDS             complement(8451..10688)
                     /locus_tag="PLS16_01865"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNJ59851.1"
                     /translation="MSNFNISPKIIWKFFFAGLGFVFLFFLLIRIGLFGKLPSFEEIE
                     NPNSNLASEIYSADSVLIGKFYVNNRSNVSFDQLSPWLEKALISTEDKRFYEHSGIDI
                     FRTFKAVFTLGQDGGGSTITQQLAKNMFHKRGGGIITRVIQKAKEYVIAVMLERRYTK
                     EEIIAMYFNTFDFVNNAIGIESASRIYFNKQPKDLNIEEAAMFVGMLQNPSLYNPNRN
                     ETFRKRCEERRSIVLRLMRDNGHISNAQLSELLMKPIELNFHPESASDGLAPYFRQTL
                     AEYLKKWAKENKKPDGTSYNIYTDGLKIYTTLDTRMQKHAEEATVEHLRNHQKIIDAQ
                     FRSGWNPWKKELGQKILLSACKNTPRWRNMKAEGMSDDAILEVFKTQKNKMTVFSYNG
                     MKDTTFTPYDSVRYYKSFLQVGFMVMEPNTGYVKAWVGGPNFNYFKVDHCNTQRQVGS
                     TFKPIVYALAVDNGWSPCMVVTPGKVTIGNWSPRGGSGGPQTLKHALTKSDNQVAAYI
                     TLKFGVSAIIDMSRKMGIVSDLPPYAPISLGASDITLSEMMTVYSTFPNAGISTKPQF
                     LVRIEDKDGNIVQNFTTEMREVLSDNIAYKMVDMLKGPVGPGGTGGSLRGRFGLGDIK
                     NICGKTGTTNNNTDGWFIGFTPQLVAGAWVGCDDPILHFLTTGNGMGAASAMPIFGKF
                     MYKAYNDPKLKKLKKDMDFFIPSDAELAKNVCSGDEVETDIYNSTVGGNTDAVEENYD
                     TEYKF"
     gene            complement(10681..13878)
                     /locus_tag="PLS16_01870"
     CDS             complement(10681..13878)
                     /locus_tag="PLS16_01870"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ59852.1"
                     /translation="MLIRKSIYIIVFLCTFFLPQQSFGKGKKKQPSQYKKATLIQKAW
                     GDLTTRNNWYFNANERYKELLRQNALNFKIDFSKEIPFYWANKDNLKSYTSELQQIEK
                     KMGIVLQIRNYGRWRDNAYLLLGKAQYFQNKIDTSLITFQYIVTTMKPEKMNAQVGLS
                     NKARLKYLQKRQKEIKKKAGENQKIIELKLSQQKKDAAKKADELKQKQAEAIANKKKS
                     IEEIIKAKKKIIALQKKGKKIPPELLAKVKKSSTPIDSTAIKQKQQEVQIQKKQEQNK
                     NQPYILLDDEWIKNPYYIDTTQNRKDENPLADLSKKEDAKWDKLTFWEKIKHKKSRPE
                     ALVWMARSLIDMNRYADAQSMIAYAKALRKLTTNQKKDIFLISAYYQLNSKNIPLTIE
                     ELENAMPFIKKKQEKAYYEFLLAQLYKQNDQPADAVDYFVKNIKHTNDDKFAFYSKLK
                     MADIFAKYPGLSDANIEKMLAKLVRFGKNKDQADEVYYELANYALNNNKDTAQALIYL
                     DKSVKLSTSNSIQKGLSYLLMGKINFDTEKYAVAKLNYDSAVAFIPKENFEYPEAITR
                     QSVLDELAKNREIVSVQDSLQRLGKMSPKELAQFLAEKEAEKEKINKKKSRISIEDNV
                     GGGNVDLSAGFTPQNYSSNGLWYFYNDELKSKGYNQFKAVWGNRPFEINWRRSSKSIF
                     DTEDGENTTENVPAENENLSTDKNSKQPTKETKVDIPKTPEDFIASDKKIANALFNIG
                     VVFKNKLQNIPKAKQAFDELIRRFPNSEFDAIAHYYQYLIYLEQDLKGLASKEKNYIL
                     ENYPLSDVAAKLQEQPQKVQETTPTQNQNEALYAQTYQAYLNDDFETVLNNKKLAQKS
                     KMPDEEMAKIDFLEAMVYGKQKVFKKYKLALSDIVLKYPNTEVKKKAQEYLIALIQFE
                     NGLEDSTKTVQEMIITPQKSDTTNIEQIEFNMDTSDFMIVVRLNDSYFKLQQVVSDLQ
                     GYCESKASNQKVKVNPVFFEKGEALITLKRFDDMEIVSKLLLNLNIDKQKLFNEKANS
                     VSYYVISTDNFKKIKSFKDLDTYEQFFTKKYINNE"
     gene            14046..16073
                     /locus_tag="PLS16_01875"
     CDS             14046..16073
                     /locus_tag="PLS16_01875"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HNJ59853.1"
                     /translation="MKKTFLILFTISVGLVSCTNNQKIKTDPLVEAIDTTIAADDDFF
                     MFANGKWIKEHPIPDAYSNWGIGNLVQEQIWEQLKKTNENAQKRANNTTEQKIGDFYT
                     SAMDTNTIEKNGLKDLQPELDRINKISNLKDLTEAVAYFNTIGVPLFFDFGIYQDLMN
                     SDKNIIYLYQGGLGLPNRDYYFNTDQRTSEIRKEYQNNFIKNIFTFSTDKTTEFNFSA
                     DAIFKLEEKMAKSSRKLEDLRDAYANYNKKSIRDLQKLTPNFDWNLYFKELNIQNIES
                     VIVGQPEFFVALNNLLQEQNLKTLKNYMQYHLIANYAPYLNKAIDEVDFNFYSKLIKG
                     KKEQLPRWKRALIWEEDAMGEELGKLYVKENFSETAKKRYIDIVKNVMTAYEKRIKLL
                     IWMSDSTKQKAIEKLHSITYKVGYPDKWKDFSKLEISKNSLVQNQKNISKFWLDDAIN
                     KLGKPVDKSIWEMTPQTYNAYYNPSNNEIVLPAAIFTVPGYKDYQLDDALVYGYAGAS
                     TIGHEITHGFDDEGKEFDSKGNLNKWWTATDDKMFEEKTKAYVNVFDNLKVLDSLKVN
                     GSATLGENIADLGGIAIALDAFKQTKQYKENQLIDGYTPLQRFFLGYALGWRMHMRNE
                     EIANRVLTDVHALYFHRVNIPFSHTPEFYDAFNIRPDSKMYVTPDKRVKIW"
     gene            16067..17227
                     /locus_tag="PLS16_01880"
     CDS             16067..17227
                     /locus_tag="PLS16_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59854.1"
                     /translation="MVEKQEIKCPNCGSNQIKTEFGEVRVCLNCNHKFNVHLVQKNSP
                     KIILIGILFIVGLIAIIQYTIPKFDEQKAMEELMQQQTQTSQKNESSKRKIITEKIEF
                     IHNNKIWKSRIDANSDLITISLQIIDADNKKIDDEIALDVIQVKEDNPYDLVTVPLEK
                     SLFAIINQNKIIEINIASKEIVAKNNAFIAKYKELKNGIQKIEYSKAMQAIIFQNLDG
                     ATFYYFPKTQLFVSNIELTQLRKMEQTGDNWKFEKKYIISNGKLFAITKKVQQYTMEL
                     SSSDIQTILEGADWMKKNYAIVAIEPNLKNVDFNNTEILYSNETEFVCVNEPKNEVVY
                     YNLKTNKTWHRKIDDIQNKQLQAAISRSEVLIYHSNNAYAFDKKDGNLIWKY"
     gene            complement(17229..17927)
                     /locus_tag="PLS16_01885"
     CDS             complement(17229..17927)
                     /locus_tag="PLS16_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59855.1"
                     /translation="MGFVQNIRQKIVDFIVKKAIENRTIDILEVRTKAINLTYVDLET
                     DIKISNSFFLPIKVLEIKTDIVNTAELKIGALHYNKVQKINAHQSAIFTTHTQISNIT
                     ALFNLISKLLTLSIKMRSVGVAKIKVFWFVVEIPVDDVFEIKSHQLRIIGELTPEEKA
                     EREALKKIKKDAYEQKKTERKQKRLEIKELKRQKSAESEDAENTLDITINEDALKSME
                     KELDDELNRQKGKE"
     gene            18059..19255
                     /locus_tag="PLS16_01890"
     CDS             18059..19255
                     /locus_tag="PLS16_01890"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNJ59856.1"
                     /translation="MLQTSDTLNRMSESATIKMAQLSRELAAKGVHVISLSLGEPDFQ
                     TPKHIKEAMKTAIDSGKYDSYSPISGYADLKQAICDKLKRDNNLDYKPENIVVSTGAK
                     QSLANVFVAFLNEGDEVIIPAPFWVSYPEQVKMCNATPIYISTNVDAEFKITAEQLEN
                     AITPKTKFLVYSSPSNPTGSVYTREEIESFVKVLERHPQVYIVSDEIYEHINYVGKHV
                     SIAEFPSIKDRVILVNGQAKGYAMTGARIGYIAAPAYIAKACDKLQGQITSGTNSIAQ
                     RGTIEALLGDQEPTYAMREAFKKRRDLVLSILNTIPNIKCNIPTGAFYVFPDVSAYFG
                     KTFEGEVLKDADDLCLYLLNVGHVAAVSGSGFGAPECIRFSYAASEENLKEAFTRVKN
                     ALEKLA"
     gene            complement(19310..19912)
                     /locus_tag="PLS16_01895"
     CDS             complement(19310..19912)
                     /locus_tag="PLS16_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59857.1"
                     /translation="MKYFIFSVLVMSILGNLQAQNDWALERDKNGIKVWTKKRPNSSL
                     KEYKGVTVINATVDELVSIFKNTKLHDQFLYKAQAGSVKVVKKISDNDFYTYMVVNAP
                     IVKDRDVVTHYKLIKDNNTGTVTVELEAAPNLVPINDNYVRVPEMKGYWKFIPKGNST
                     EVIHQAYSLTGGNIPSGLANSAAVDTPFSMLSELKNLAEK"
     gene            20014..20955
                     /locus_tag="PLS16_01900"
     CDS             20014..20955
                     /locus_tag="PLS16_01900"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNJ59858.1"
                     /translation="MKTYKLIFGFLLLIILPTSFAQGYLGVLVKDYDLSGIKGALILN
                     ILDDGAAKNSGLKENDIIAQLDNNAITNKSDLINLLNKKNWGDKIHLQYYRNGSLNHV
                     DIILGYKRNTKTYNVHKKIIDKNQHWIFEDDNTVIVIDKTNQPISINKIGNGCLLDTW
                     YIQQDYKDDEIPQCYLDLDDKLYTIERIKASQEKRKVTIDNIIYIKKITEGEQSKSKT
                     EAIEKPILDFHTFGISPNPSDGLFTISIDTKSTTSFGIKILNASGLAVHETNVSSSGA
                     GIIKQFDLRNLAKGIYLIQLAEVGNEQNVIAKKIIFK"
     gene            20962..>21205
                     /locus_tag="PLS16_01905"
     CDS             20962..>21205
                     /locus_tag="PLS16_01905"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HNJ59859.1"
                     /translation="MAEIGSNIEIAADWLKKGNVVSIPTETVYGLAANALNETAVLQI
                     FDIKKRPSFDPLIVHISSINEIEKYTLSFPEVLKPLA"
     assembly_gap    21206..21305
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            21359..23155
                     /locus_tag="PLS16_01910"
     CDS             21359..23155
                     /locus_tag="PLS16_01910"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNJ59860.1"
                     /translation="MSKEEASELKYQAPETKYFKSKEEFNEAVAKDFIEYANRSLKKG
                     EKFLVGMSHGKSPSGAYKYVVKHFDKIIHPHNLYFTFVNSPLKSQESVELETMDARSF
                     IKTLLKLGLIKIEQVLGKRFNEAEIEQYMLEFNQTVQKFLDENNKEGLDYAMITSNPK
                     GRIAGIERKSKAFESNEITTIVTIDKEKELTCTPSFLLKTKRIAFLATKADKRRPLAW
                     LYSTDGQADESPSFIRFIDTNKQKITVFVDDKALTWPQIEIYRETPYGVTTIRLDTTY
                     PYKENSKEKLPVLIMVHGFLGLNSFDGLLTKLPTTKYIAAAMHYGTIPDDLPVKEYSN
                     HVMLNIDAVIQYFGSKGHPVYLFDHSMGNTYFLMMDREYDNLVGVKKYLKGRIGANPF
                     FGEEVKHAILGFMDYVIIPSMKFVNAPIETSFLAMFRRIVPLDSKFGVRKRAMNLASM
                     VIGKEHTENSAIWSAIKARLLYQMGNLDSVPELNRIPIEKAMNRIPPKIFAIQSSSAL
                     RESKKFDKQVGLTNTLKHHLPVMILKSELDGVAKYVPRLYESENVKVVDVTNGSEHDF
                     FREHLYHMVEPIHTSQLISEFIDEIEANNKKT"
     gene            23233..23772
                     /locus_tag="PLS16_01915"
     CDS             23233..23772
                     /locus_tag="PLS16_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59861.1"
                     /translation="MKHERYYYNPKTLRYEKYKLTVVQRVIRVIGFFTSAFFFGFLAM
                     ILGYNFFETPQQKTDSLKIKSIQDRYDILGKRVDALASVINELGKKDDNIYRAIFEAD
                     PLPNELRQKQINRSKAYDELKNIPEGKIVAQTLAKIEDLEKKIYIQSKSYSELTQLIN
                     NKEKLLAAFLLFSLLPTKT"
     gene            23802..24203
                     /locus_tag="PLS16_01920"
     CDS             23802..24203
                     /locus_tag="PLS16_01920"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNJ59862.1"
                     /translation="MDPVYKTTRFHAGLDFSAPTGTPVYTTGDGVVEKAGSEGDGYGY
                     KVIIKHGYGYSTLYGHNSKVLVKVGQVVKRGETIALVGSTGKSTGPHCHYEVWKNGQK
                     INPINFFFNDLSPTQYDEILKIAETSNQSFD"
     gene            24351..26102
                     /gene="ric"
                     /locus_tag="PLS16_01925"
     CDS             24351..26102
                     /gene="ric"
                     /locus_tag="PLS16_01925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HNJ59863.1"
                     /translation="MQTINAPIINVQEIEPKLRHLTIFQTFDKLNPGEYLIIHNNHDP
                     KPVFYQLQEIRGNVFDWVYLQAGPEWWDIKVTKHENAPKPAVIINEKGEIEITVPALE
                     PRLKHETIFFAFEKLGSGKTMIIHNDHDPKPVYYQLQSMHGDIFTWDYITEGPEWWQI
                     RVTKKASAQPNPYINDKGEVVINVPALEPSQKHPTIFNTFERLIPGKTLVIHNDHDPK
                     PLHYHLQNMHGDTFSWEYLQEGPEWWDVKIIKKQKEENTSEPNYLKVGKNPSNQKETT
                     ITIPLIEPKLKHATIFKIFDTLEVGESLVIHNDHDPKPVYYQLLGERGETFTWEYLEQ
                     GPQWWDIRVTKKGTENDETIGEIVAKDIRKAEVLKKFGIDFCCGGNKTVRQVCTEKGI
                     DANLVEQALQQPIQNLTRNNHNYNEWNIDFLADYIVNVHHSYVKKYLPEIVGYAAKVA
                     KVHGPNHAELASINDLVAKINTELLKHLKEEEGVLFPLIKKIVQAKHSNTKLDSTNNF
                     AQVLEELEKEHEQVGEMVDKIRILSNEFAIPEDACTSYKLLYKMLQDFEDDLHIHIHL
                     ENNILFPKSIAIEKELV"
     gene            26129..26404
                     /locus_tag="PLS16_01930"
     CDS             26129..26404
                     /locus_tag="PLS16_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNJ59864.1"
                     /translation="MNFTEVHKNENYKVTEVFLELGEKMPLHQATSNAFVICRNGKSK
                     LTFFDKTLVLSQGESILIQANDPHELEVLENFSASIIMDPDAKIDFL"
     gene            26446..27267
                     /locus_tag="PLS16_01935"
     CDS             26446..27267
                     /locus_tag="PLS16_01935"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HNJ59865.1"
                     /translation="MLINEQTKIATLIKHHPDALETIVTLSPDFKKLRNPILRKLMAG
                     RTSIAMASKIGGCKPEDFFKALQPLGFEVDTKGVIPKEKEPEQKALPIYLKNAKAEQI
                     VNFDVREMLAGGSDPLRDIQLKVKDLKAGEILKIINTFEPVPLVKLLEKQGFQTYVDA
                     ISDNLYETYFFKTDGTDSSADLPTEIQNSTDWEQLLQHYKDKLREIDVRHLEMPMPMM
                     TILENLETLPEDMALYVNHKRIPVFLLTELKDRNFDFRIKNIQDGEVYLLIFKNK"
     gene            27270..28556
                     /locus_tag="PLS16_01940"
     CDS             27270..28556
                     /locus_tag="PLS16_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit I"
                     /protein_id="HNJ59866.1"
                     /translation="MIGSLVGNGLQQTTSYKVVIPFYVYAAVSLLIGTILLFFHTDIA
                     HQHYFHPYTLAITHTMALGWGTMIIFGASHQLLPVLVEGKLDSNLLAYLTFIFAAIGI
                     PFLVAGFYMFQMGWLVQLGAILINIAVICYLTNVIMSIYNSKKYNIHAWFIATASLWL
                     FSTTFFGLLLVFNFTRSILPKDSVAYLTIHAHLGIVGWFLLLVIGVGSRLIPMFLISK
                     YTNDKTLWQIFILINLSLVGFIIFKLMNCPTTYYYIVLALGLIGIALFGNFCRMAHKV
                     RIRKSVDEQMKVSLLSVLQMLFPIIALLIVIILFPSKKYADMSILYGFCIFFGWLTAI
                     ILGMTFKTLPFIVWNKVYSKKAHAGRTPVPKDLFNENIFNGMTLAYILGFILFITGMI
                     LSLNIVLKIATIALVLAASLYVSNITIILLHKPNIK"
     gene            28553..28876
                     /locus_tag="PLS16_01945"
     CDS             28553..28876
                     /locus_tag="PLS16_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HNJ59867.1"
                     /translation="MNEVTNDSIKLNIALSDLYNVYDPEIGLNVVDLGLIYKIIFEEV
                     TQRLDCEMTLTTQFCPMGESITNDVIQSLTKSFPSWDINVNLTFEPAWDYTKISPEGQ
                     EFLGH"
     gene            28879..29310
                     /locus_tag="PLS16_01950"
     CDS             28879..29310
                     /locus_tag="PLS16_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNJ59868.1"
                     /translation="MLSLTCKTAIKAVIYLSSKSVNGEKVGLKEIAEHINASEHTVGK
                     TLQTLVRHGIINSLKGPTGGFYITKAQQNQPISNIVETIEGKEIFKECGLGLSKCSAQ
                     HPCPIHNEYKVARELIEQLFREKKVKDLCDPVTNGIAYLVG"
     gene            29334..29723
                     /locus_tag="PLS16_01955"
     CDS             29334..29723
                     /locus_tag="PLS16_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNJ59869.1"
                     /translation="MGEKSNQSTPLRPQGERVLSSNLVEMDVNQFIQQIKSEKVWAES
                     DHNSITIFKSDKMRIVLIGMHEGAALKTHTANAIISVQVVEGSIKFTADEQTIALEKG
                     KMIALQEKIPHSVFAIEDSFFLLTLAM"
     gene            complement(29770..31017)
                     /gene="odhB"
                     /locus_tag="PLS16_01960"
     CDS             complement(29770..31017)
                     /gene="odhB"
                     /locus_tag="PLS16_01960"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HNJ59870.1"
                     /translation="MKQEIKVPSIGESISEVTLASWLVEEGSYVELDQPIAEFESDKA
                     TFELPAEFAGVITRAANEGDDIQIGAVVAYIETDAAKPQSTAGNSEKPEVAKTEDIKV
                     SANIQQTTNNTSYASGTPSPAAKKILDEKNIPASDIKGSGVDGRITKEDAQKAEAPKS
                     ATNVPASTKPTSPAIVAPTAVGLREERIEKLTRIRKTISRRLVEVKNQTAMLTTFNDV
                     DMSAINEIRAKYKDAFYEQNGVGLGFMSFFTRAVCLALQKFPAVNAYITDENIIYHDY
                     CDVSIAVSTEKGLVVPVIRNAETLSFAGIEREIKRLAVLARNNELSMQDMEGGTFTIT
                     NGGVFGSLMSTPIINQPQSAILGMHRVDERPVVINGNIEIRPMMYLALSYDHRIIDGK
                     DSVSFLATVKSYLENPEKMLIAL"
     gene            complement(31089..31973)
                     /locus_tag="PLS16_01965"
     CDS             complement(31089..31973)
                     /locus_tag="PLS16_01965"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HNJ59871.1"
                     /translation="MKYAAIDIGSNAVRLIFTNVYHTPEGKQYIKDAMYRVALRLGEE
                     SFTRGSFSKSKIEDMLSTMKAFKNLIDVHKPRKVMACATSAMRDASNSQEIVDLVHKK
                     TGIQIEIISGQREAEFVLSNHVELYDFKEHTNYLYIDVGGGSTEIILLSGGKTIAKRS
                     FDIGTLRMCMGKVKDKQWEKLENWLLEINSTYSNIQGIGVGGNINSIQKLFANSKKTI
                     ATKHTIETTIHKLEKLSIEERIIQYQLKPDRADVIVPAAKIFNFIMEKATIENLIVPK
                     VGLGDGMIHYMAKSEKNK"
     gene            complement(31986..33032)
                     /locus_tag="PLS16_01970"
     CDS             complement(31986..33032)
                     /locus_tag="PLS16_01970"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HNJ59872.1"
                     /translation="MQVNIPLLAKICEVPGTSGFENEVRKIVYEELKSEDLTIETDNL
                     GNVYAIKKGKDSSKRVMIGAHMDEIGFIVKYIDDNGFIRFHTLGGFDPKTLTAQRVIV
                     HGKKDLLGVMSSKPIHIMTPEERNKVAQISDYFIDLGMPADEVKALVQVGDPITRERT
                     LVEMGNCVNCKSIDNRVAVYILIETLKALKDCPYDVYGVFTVQEEVGIRGANVAAHNI
                     NPTFGFGLDTTIAYDLPGARPEEQITKLGEGVAIKIMDASTICDYRMVRFQKQIAEEF
                     QIKWQSELLPMGGTDTAGIQRMGKTGAIAGAISVPTRHLHQVIEMAHKEDISNSVLLL
                     KHCCENLDRINLEF"
     gene            complement(33198..33809)
                     /locus_tag="PLS16_01975"
     CDS             complement(33198..33809)
                     /locus_tag="PLS16_01975"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013352894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNJ59873.1"
                     /translation="MAFELPQLPYAHNALEPNIDTQTMEIHHGKHHAAYVNNLNATIQ
                     GTENEGKSIEELLANVSRLSPAVRNNGGGHFNHDLFWKVMSPNGGGEPTGKLADAINA
                     AFGSFEAFKDAFAKAAATRFGSGWAWLAVTADKQLCVTSTPNQDNPLMDVADCKGTPI
                     LGIDVWEHAYYLHYQNRRPDYVNAFFNVINWAEVAARYEQAIA"
     gene            34068..>34384
                     /locus_tag="PLS16_01980"
     CDS             34068..>34384
                     /locus_tag="PLS16_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59874.1"
                     /translation="MNFKYQFFDSREGFVKLSKLVIKANGKKIGESPAKLENIENKVS
                     VQLPKGNYEIDATVYSMYNGVWEERTEANDYTMDCKYIIDSLLLESDNNITLTFNAVT
                     GYK"
BASE COUNT        11825 a         5407 c         5238 g        11814 t
ORIGIN      
        1 atttgtccag gtaacacagc agctacattt tgttgaatta actgttgtat tttaatattt
       61 gtttgttgtt ggatagtcgc gattcccgct ggagaggaaa tatatccatc agcttgagca
      121 tttgcagttg cctccggagt tggatcgctt gctgcaacag cagcggcgta agcttgatta
      181 taagcatttt gtcttactac tgctgtaaca ccagcctcaa tagctggtct atttgcattt
      241 acttgttgtg taactgctgc tgtaacttgt gccgtaaccc cagctgttac cgcaccaaca
      301 aaagcaggat tattttgtaa agagcctgaa gtacttgcaa ttaagttacc tctaattcca
      361 ccataaggaa cttggattgg tgtttcatca ccataaacat tcataccatc atagtttggt
      421 gcaccaagtg gaccattgtc atctcttgtg gacatatcgt ttgcatacca gtcagttcct
      481 tgaaatgcat ttgcattaaa cttaatagcc acatagtcct gtccttttct tttccataca
      541 tgaccaacac gcattgccac acttcctaat ggtttggtac catagccatt actagcactt
      601 gcaaaaccac cttttacttg tgcagagaaa cctggagagt caaaaggatt ttttgaattc
      661 attaataaaa taccattgaa agcatttggt ccatataatg cagaagctgc acctggcact
      721 aattcaacat tctgaacgtc caactcacca ataccaacga tatttccagt agggaaattt
      781 aatagaggtg cggcattatc cattccatcc attaactgaa caaaacgtgt atttgaaatt
      841 ccaccaaatc ctcttgtatt tactgatgtt aaattcattg aaccattaat aacctgaaca
      901 ccttttaatt tagataaatc atcatagtaa tcattagaag atgattgctt taccattttc
      961 tgatccatct tttcaatcgt aactggagat tctaaaatct tctcctctac tcttgacgca
     1021 gaaactacta ctgtatttaa aatattgtgt tcttctttca ttccaatatc taagccagat
     1081 gtacttcctg taatttcttt ttctacattt tccattccta ccattgaaac aacaatagta
     1141 aatggagttg attgttcagt agttagttca aaattaccat caatgtcagt aatcgttcct
     1201 tcagatgagc ctttgatgct aatatttgca ccaattacag gctcttttgc ttcatcagtt
     1261 accttgccac ttatgacagt ttgggcataa gcaacaaaac tgaccagcaa catcattaat
     1321 cccagtaagt tttttgttaa tttgttcata ataatttgta tttatctgtt gcaatataca
     1381 ataagttttt aataatgcta atttttgtta atgttttttt aatcaatatg aaataataaa
     1441 tcaataaatg gctctttatt attaattata agtaagtata ttgtttagaa atgttgtatc
     1501 ccacaattct tgcttatctt tacaaactat tagaaaatat taagtttcaa cgttttttat
     1561 gaactatctt tccgtccaaa acctaagtaa atactatgga gaacgtcttt tatttaaaga
     1621 tattacattt tttattgaca aaggtcaaaa gattgcttta attgcaaaaa atggttcagg
     1681 aaaatcttct ttaattaaaa tcttacaagg catagagcct cctgaaagtg gcgaaattaa
     1741 atttcataaa gatataaaag ttggtttttt aattcaagat ccaatttttg acaaaaatca
     1801 gactgtttat gaggcaattc tcaatactga taatgagctt ttgaaaactt gtctaaacta
     1861 tgaacaagca ttggctggaa atggtaatct gaatgatgcc atggaacaaa tggaaaaatt
     1921 gcaagcttgg gattatgagc aacaaatcaa acaaattctt tcaagatttt taatccatga
     1981 tttagaacaa aaagtaaata ctttctctgg tggtcaaatt aagcgtttag ctttggctat
     2041 tttactatta caacaacctg atttattaat ccttgatgag cctaccaacc atttagattt
     2101 agatatgatt gagtggctgg aagggtattt atcgaaagat aatataactg ttttgatgat
     2161 tacgcacgat aggtatttct tagaaaatat ttgtactgat attcttgaaa tggaagataa
     2221 tattttatat aagcataaag gtaattattc tcgttattta gaaaataaag aaattagaga
     2281 agaacatcaa cgtgtgaatg tagaaaaggc tcaaaatatt tacaaaaaag aattggaatg
     2341 gatgcgacgc caacctaaag ctcgaacaac taaggcaaaa tcaagagaag ataattttga
     2401 gaatgtaaaa aaagccgcta cccaaaaagc tcaagagaag aaagtagaaa ttgagattaa
     2461 aatgaatcgt ttgggtggca aaatcgttga attacataac atctctaaaa agtacggtca
     2521 gaaagttttg ttggataaat tttcatataa atttcaaaaa ggcgagcgag tcggtattgt
     2581 aggcagaaac ggttgtggaa aatctacatt attaaatatt atcattggca atgataagcc
     2641 agatgaaggc aatattgttt taggcgaaac tatagttttt ggttactaca accaaaatgg
     2701 aatgcatcta aaagaagaca aacgtgtcat agaagtaatt aaagatattg cagaatatat
     2761 tcctgtagct ggaggacaac tttcggcagc acaactttta gaacgtttcc tatttgcacc
     2821 cgaacaacaa tataattatg tctccacatt gagtggtggc gaaagaagaa ggttatattt
     2881 attaaccatc ttaataaaaa atccaaattt tttaattctt gatgagccta ccaacgattt
     2941 ggatattctg actttaaatg ttttagagga atttttatta gattataaag gttgcttaat
     3001 tatcgtttca cacgaccgtt attttttgga caagctcacc gattggcttt ttgtctttga
     3061 aggtaatgga aagattaaaa cctttttagg aaactataaa gaatatagag atgatttgaa
     3121 agagcaatta cgcttagaaa aaggaaatca aactaaaata gtttctaata attcaaaatc
     3181 aacaaatgaa gttcctaaga aaaaactaaa ttttaaagaa caacaagaat ttattcaatt
     3241 agacaaagat atttcaacgc ttagtgagga aaaagataca ttaacagctc aattgagctc
     3301 aggagcaata gaccataatg aaattcagaa tatttcaagt cgtttacaag aaatcattga
     3361 cttacttgat gaaaaagaaa tgcgttggtt agaattaagt gaatttgctt aatcaatttt
     3421 ttaaaccatt gatggcatat aatttgaagt aaaatagcgg atgcaaaaaa gactttccca
     3481 taattattat tgcttattgc tgaatattat agtatttgct ttatgtctga ctactttaaa
     3541 tagtaatgca aatgacttaa ataaaccatt tttaagtttt gaattattgc ctactgcttt
     3601 caaaaaacaa ttaaacattg taaatagtga atttaccttc gcttatggaa tgcgtcaaga
     3661 ttctgtgatg cgtgatattc gaaattatac aaaagaagat attgaaactt ttatacattc
     3721 aagaaaagat tacgaatata ttttcagaat tccattttgg gcagctatgg atagtataag
     3781 atatagttgg ctcattccta aattaatcaa cgatttatcg gacactacat ttgttggatt
     3841 aattaatgcc agtgatgtta ctatttggag tagagtaaaa acaggtgata tggtacacaa
     3901 taccctaaac tatcaaatag atgatgatat atttacagtt gcaggacgag ccagttggat
     3961 tcttaaaagg ctttctaaaa atgagtacgg aattataaaa cctaatatgc cgagcaaaga
     4021 aattaaagag ataataaata ggtggaataa atggtacaac acactaatta gaaagtaaat
     4081 tatgtatcaa taataaaagt aaacgccact ttaatcaaag cattttaaca actatttaca
     4141 agccaatcaa aataatctat acttttttac tatttttgag caaaaaatag ctgaatgaaa
     4201 ctaaagcagt ataagattat caatacttta ttagggttaa ttgtattcgc catttcatta
     4261 tggcaatatg cttctaccgc agaatcggca ggaagtttat gggattgcgg tgagtttatt
     4321 tcttgtgcct acaagttgca aattgctcac cctcctggtg cacctttatt tttgatgatg
     4381 ggaagggtat tttccttatt tgcatcagat ccttcacacg ttgcatttat gattaacctt
     4441 ttatcagcag tcatgtcggc tggagcggtt atgttcttat tttggattat tacaataatc
     4501 gcaaaaagaa ttgttgcacg caatgaagaa gatttaacta cagacagagt aatatctatt
     4561 atgggcagtg gtttggttgg tgctttagct tgttcatttt ctgattcagt ttggttttcg
     4621 gcagttgaag gcgaggttta tgccgcatca ttatttttca tttctatcac tttctgggca
     4681 gtcatgaaat gggatgaaga agcagatgaa ccacacgcta acagatggtt ggtctttgca
     4741 agttttatga taggcttatc tattggtgtt cacttgcttt ctttattagt gattccagct
     4801 gctgctttaa tctattattt tagaaaatat gaaccaaata ttaaaggatt tataatcgct
     4861 tttatagtag gtttctttgt attaggatta gtacaagttg gtgttgtatc tacagctcca
     4921 ttaatagcaa gtaaatttga atactttgct gtaaatattt taagattacc ttttaattca
     4981 ggtttaattt ttgctattat cttattcttt gggattttag tagccattat acgttatgcc
     5041 aataaaatca aacatgcaga tatgcaatta tctgcaattt gtgctttagt gatttttatt
     5101 ggcttctctt cttatgctat ggttccaatt cgtgctacgg catttcctcc aatcaatatg
     5161 aacaacccat ctgatgccta ttctcttatt tcttacttaa acagagagca gtatggcgaa
     5221 agaccattgt taaaaggccc aatgtttgac gctcaacctt atgctattga tgaaaaaggt
     5281 gaagttcttt atcctaataa ggaaacaaaa aaatatgagg ttaaagacat caagcgtgct
     5341 tataaatata aagatgagga aatgttgttt ttcccaagaa tgggaccaac aaatgatgga
     5401 gcaaatgcaa aacaactgtt tcaatattgg acagattttc aaggaacacc aacattcagc
     5461 gataatataa gttacttttt atcttatcaa gttggctata tgtattggcg atattttatg
     5521 tggaattttg ctggacgaca agatgattat caaggaatgg caggtacaca aaaaatcaat
     5581 ggcgattggc tttcaggcct ttcatttata gacaattcaa gattagggtc tcaagaaaat
     5641 ttaccttatc aaattaccaa tcagaaagca agaaataaat tttatttact gcctttaatt
     5701 ttaggattaa ttggcttagt ttatatggtt aatagaagca aagaatatgc tttagtttta
     5761 ttgctcttat ttttaaatac tggattattt ttaataattt atattaatga accaccaaga
     5821 gaaccaagag aaagagatta tgcattagtg ggttcatttt ataccttctg tatatggata
     5881 ggctttgcag tattggcagt tgcagaatgg atacgtgaaa aatctaaagg actagcatta
     5941 cctgtaagta tagctgtatc acttttattc ttgggcgttc cttatattat gggaaaagaa
     6001 ggttggaatg atcacaacag acataataga tttatggcta gagattttgc cagtaattat
     6061 ttagaaagtt gtccgccaaa tgcgattcta tttacacaag gcgataatga tacttaccca
     6121 ctttggtatg ctcaagaggt tgaaggtatc agaacagatg taagaatcgt aaatttgagt
     6181 ttattaggtg ttgattggta tattgacggg ttatttagag cagaaaacaa atctactcca
     6241 attcctctgc aaaaagattt tacgcctgac aaatacagag gaagtacaag acaagtgctg
     6301 tcttataatc ctcaatcggg ttttgcagac ccaaatcaat attatgattt atcgaatatc
     6361 ctttcttttg ttttgagtga tgatatgaaa tataaagctc aaactcaagg tggcgaaacc
     6421 gttaattatt ttccaagtac taaattcaaa ttaaaagtaa ataaagaagc tgctcttaaa
     6481 aataatgtag tacctgataa gtacaaagac ctaatcacag atgaaatagt ttgggattta
     6541 ggaaaagaca gagttattat ctatgattta gctgttttag caatgattgc tgctgaagat
     6601 tggagcagac ctatttgttt tgcaaataca gtagaatcct cctattatca aggtttggat
     6661 aaatatatga ttcaagaagg attgattaat agaattgttc ctgtaaaatt tgaagaaaac
     6721 caacgtggtt ttatggctat gaatacagat aaaaattttg atttagtaca aaacaaattc
     6781 aaatatggcg gattaggaga aagagaaatg tttgttgatg aaaattccgc taggattatg
     6841 aatagtcaaa aaagtgtaca tctttcaata gcagatgatt taattagaaa tggaaagaat
     6901 gatgaagcaa ttaaagtact agataaagct aaaaaagaat atcagtatgt aaatgctcca
     6961 tattacgctc cgaataatcg tttctttaat attttagcca ttcaatggat agatttatat
     7021 tatagagctg gaaagcctga aggtgcgaaa gaaatcaaag aattattttt aaaagatatg
     7081 gctgattgtt tgagattcta tttactgcca aataattctt atgctgaaag atatgctgat
     7141 gagaaaaaat ctgctgaaga tatgattcaa agaatgcaat atttagctat taattataag
     7201 gataatgatt tcatgaagca gttgaatcaa gcttttccaa attatgttca gagtccaaca
     7261 atagaaccta atatggttgc tccgcaacaa atatttagat aattttttaa tcattaaaaa
     7321 caaaacatta tgtcattact aaaagaattt aaagagtttg cactcaaagg caatgtgatt
     7381 gacttagcag ttgctgttgt tatcggtggt gcatttggta aaatagtatc ttcactaata
     7441 gaagatataa ttacccccag taattttaaa tcctgcacta aaagccgcag gtgtagatca
     7501 gattgcacaa ttatctgcta atggtataaa atatggtgta ttcctatctg ctgtaatcaa
     7561 ttttattgta attgctttct ctatgttttt agtagttaaa tctatcaata agatgaagaa
     7621 ggaagaaaag gaagctccag ctgttgcacc tgaacctact aatgaagaaa aactacttgg
     7681 agaaattaga gatttattaa agaaataaat tattctataa atattaaaac ggtcgggatt
     7741 atttccgacc gtttttattt tagtgcttac gttcctgaaa ataattatgc gttatagaat
     7801 cctcatactc tctgtgataa atataagttg gagaattcat ctttaagata ttctttttag
     7861 cacgaactat cctatcgaat aaatctgcat cagcaccaat atcaattgtt ctaaagcctc
     7921 ccaattcaaa tagcacctct ctttttataa agaaagtacc accaataacg caatcagaaa
     7981 gatggattct ccttccctta tttcttctat ctggcacaaa ctcatcacca ataacctcta
     8041 ctccaccgta caataaatca accattgggt gttgatttat tatttgactt ctatattgta
     8101 ggtgttccat tttataagcg tcatcagaat ctaaaaaggt aatatactcc ccgcatgaca
     8161 aacttatccc tctgtttttt gctgacactt ctcctctatt ctcttgtttg aaatagtaaa
     8221 cagattgatt gatattcaaa tagggttgaa taagtttttc agtattatct gtactgccat
     8281 cgtcaataac caataattcc caattacttt cagtttgttg aattacagat aaaatcgcac
     8341 gttcaatata ttgctctcta ttataagttg ttaagacaat agaaaactta ggaataaaag
     8401 cactcaagga caagttattt attgtataac aataatctta acttttagtt ttaaaactta
     8461 tattcagtat cataattctc ttcaaccgca tcggtatttc ctccaacagt actattataa
     8521 atatcagtct ctacttcatc tccagaacat acatttttag ccaattctgc atcagaaggg
     8581 ataaagaaat ccatatcttt tttgagcttt ttcagttttg gatcattata ggctttatac
     8641 ataaattttc caaatatagg cattgcagat gctgcaccca taccatttcc tgtagtcaag
     8701 aagtgtaaga ttgggtcatc gcaaccgacc caagctccag caaccaattg tggtgtaaat
     8761 ccaataaacc atccatcggt attattatta gttgttcctg tttttccaca aatattttta
     8821 atgtcgccca atccaaatct acctctcaag cttccacctg tcccgcctgg accaactgga
     8881 ccttttaaca tatcaaccat tttgtaggct atattatctg acaatacttc tcgcatctct
     8941 gttgtaaaat tctgaactat attaccatct ttatcttcaa ttctaactaa gaattggggc
     9001 ttcgtactaa ttccagcatt tggaaatgta ctataaacag tcatcatttc tgacaatgtg
     9061 atatccgaag ctcctaatga aattggtgca tacggtggca agtctgatac gatacccatc
     9121 tttctggaca tatctataat tgcagagact ccaaatttta atgtaatata agcagccact
     9181 tggttatccg atttggttaa tgcatgcttc aaggtttgtg gacctccact tccgcctctt
     9241 ggactccaat ttccaatcgt tacttttcct ggcgtaacaa ccatacaagg cgaccatcca
     9301 ttatcaactg ccaatgcata tacaattggc ttgaaagttg agcctacttg tctttgcgta
     9361 ttgcaatgat ctactttaaa ataattaaaa ttaggaccac caacccaagc ttttacatat
     9421 ccagtatttg gctccattac cataaatcct acttgcagaa aagacttata atatctaaca
     9481 gaatcataag gtgtaaaggt agtatccttc attccgttgt aggaaaatac cgtcatttta
     9541 ttcttctgag ttttaaaaac ttctaatatt gcatcatcac tcatcccttc cgctttcata
     9601 tttctccaac gtggtgtatt cttacaagct gataataata tcttttgtcc caattctttt
     9661 ttccatggat tccaaccact tctaaattgt gcatctataa ttttctgatg atttcttaaa
     9721 tgctcaacag ttgcctcttc agcatgtttt tgcattcttg tatctaaggt agtgtagatt
     9781 ttcaatccat ctgtataaat attataggaa gttccatctg gtttcttatt ttctttagcc
     9841 cattttttta aatactctgc taatgtctgt ctaaaataag gtgccaaacc atcactcgca
     9901 gattcaggat gaaaattcaa ctcaattggt ttcatcaata attctgatag ttgtgcatta
     9961 gaaatatgcc cattatcccg cattaatcta agtacaatag accttctttc ttcacatctt
    10021 tttctaaatg tttcatttct atttggatta tataaagatg gattttgtaa catcccaaca
    10081 aacattgcag cttcctcaat attcaaatct ttaggttgct tattaaaata aatccttgat
    10141 gcactttcta tacctatggc attatttaca aaatcaaatg tattaaaata catggcaatg
    10201 atctcttctt tggtatatct tcgttccaac attacagcaa taacatattc tttagctttt
    10261 tgaattactc tagtgataat tccaccgcct cttttatgaa acatattttt ggctaattgt
    10321 tgtgtaatgg tacttcctcc accatcttgt ccaagtgtaa aaaccgcttt aaatgttcta
    10381 aaaatatcaa ttccagaatg ttcataaaag cgtttatcct ctgttgagat gagtgctttt
    10441 tccaaccaag gcgataattg atcaaaagaa acattacttc tattattcac ataaaattta
    10501 cctatcaaaa cagaatctgc agaatagatt tctgatgcta agtttgaatt tgggttttct
    10561 atttcttcaa aagatggtaa tttaccaaac aatccgattc taatcagaag aaaaaataaa
    10621 aaaacaaaac ctaaaccggc aaaaaagaat ttccaaatta tctttggact tatattaaaa
    10681 ttactcatta tttatatatt tcttagtaaa aaactgttca taagtatcta aatctttaaa
    10741 agattttatc tttttaaaat tatccgttga tataacataa tagctcacag aatttgcttt
    10801 ttcattaaat aatttctgtt tatctatatt caaattcaat aataatttac taacgatttc
    10861 catatcatca aatcgtttca atgtaattag cgcttctcct ttttcaaaga aaactgggtt
    10921 cacttttact ttttgatttg atgcttttga ctcacaatat ccttgtaaat cgcttacgac
    10981 ttgttgcaat ttaaaatagg aatcattcag gcgtacaaca atcataaaat cactagtatc
    11041 catattaaat tcaatctgct ctatattggt tgtatctgat ttttgtggtg ttattatcat
    11101 ttcttgtact gttttggtcg aatcttctaa tccattttca aattgtatca atgcaattaa
    11161 atattcttga gctttctttt ttacttctgt atttgggtac ttcaaaacaa tatctgacaa
    11221 ggccaatttg tattttttaa atactttctg tttaccataa accatcgcct ctaaaaaatc
    11281 aatttttgcc atttcttcat ctggcatttt gctcttttgg gctaattttt tattattcaa
    11341 aacggtttca aaatcatcat taagataagc ttgataagtt tgtgcataca aagcctcgtt
    11401 ttgattttgc gttggtgttg tctcttgtac tttttgtggt tgttcttgca gctttgcagc
    11461 gacatcactc aatggataat tttctaaaat atagttcttt tcttttgatg ccaatccttt
    11521 taaatcttgt tccaaataaa tgaggtattg gtaataatgt gctattgcat caaactcact
    11581 atttgggaat cgtcttatca attcatcaaa agcttgtttt gctttaggta tattttgtag
    11641 tttattttta aaaacaacac cgatattaaa caaagcattc gcaatttttt tatcagaagc
    11701 tataaaatct tcaggagttt taggtatatc aacttttgtt tctttagtcg gctgtttgct
    11761 atttttatct gtacttaaat tttcattttc tgctggtaca ttttctgtag tattttcgcc
    11821 atcttcagta tcaaaaatag atttgctact tctacgccag ttaatttcaa atggtcgatt
    11881 tccccaaact gctttgaatt gattataccc ttttgatttt aattcgtcgt tataaaagta
    11941 ccacaaacca ttagatgaat aattttgagg tgtaaatcca gcacttaaat ctacatttcc
    12001 gccaccaaca ttgtcttcta ttgaaattct actttttttc ttatttatct tttctttttc
    12061 tgcttccttt tcagctaaaa actgggccaa ctcttttggc gacatttttc ctaagcgttg
    12121 taaactatct tgaacactta caatttctct attcttggct aattcatcta atacgctttg
    12181 tcttgtaatt gcttctgggt attcaaaatt ttcttttgga ataaatgcca cagcactatc
    12241 ataatttaat tttgctactg catacttttc tgtatcaaaa tttatttttc ccatcaacaa
    12301 ataggataag cctttttgta tggaatttga agtactcaat tttacagatt tatctaaata
    12361 gattaatgct tgagctgtat ctttattatt atttaaagca taatttgcca gctcataata
    12421 aacttcatct gcttggtctt tattcttgcc aaatcgtaca agtttagcca acattttttc
    12481 aatattagca tctgacaaac ctgggtattt tgcaaaaata tctgccattt ttaatttgga
    12541 ataaaaggca aatttatcat catttgtatg cttgatattt tttacaaaat aatctacggc
    12601 atctgctggt tggtcattct gtttatataa ctgagctaat aaaaattcat aataggcttt
    12661 ttcttgtttc tttttaatga atggcattgc attttccaat tcttcaattg tcaatggaat
    12721 atttttacta ttcaattgat aataagcact tattaaaaaa atatcttttt tctgatttgt
    12781 tgttaattta cgaagtgctt ttgcataagc aatcatactc tgtgcatcgg catatctatt
    12841 catatcaatc aagcttcgag ccatccagac caatgcttct gggcgacttt tcttatgttt
    12901 aatcttttcc caaaatgtca atttatccca ttttgcatct tcctttttag acaaatcagc
    12961 caaaggattc tcatctttcc tattttgtgt tgtatcaata taataaggat tttttatcca
    13021 ttcatcatct aacaaaatat aaggttgatt cttattttgc tcttgtttct tttgaatttg
    13081 tacttcttgt tgcttttgtt ttattgcagt tgaatcaata ggtgttgatg atttttttac
    13141 ttttgccaat agttccggtg ggattttctt tccctttttc tgaagtgcaa ttattttttt
    13201 ctttgcctta ataatttctt ctatagattt tttcttatta gcaatagctt ctgcttgttt
    13261 ttgctttagc tcatcagctt ttttagcagc atctttcttt tgttggctca gttttagttc
    13321 aattattttc tgattttctc ccgctttttt cttaatttct ttttgtcttt tttgtaggta
    13381 tttcaatctt gccttattgg ataaaccaac ttgtgcattc attttttcag gcttcattgt
    13441 ggtaacaatg tattgaaatg taattaaaga agtatcaatt ttattttgaa aatattgagc
    13501 ttttcctagt aacaaataag cattatctcg ccatcgacca taattcctga tttgaagaac
    13561 aatacccatt tttttctcaa tctgttgcaa ttccgaagta taactcttta agttatcttt
    13621 attcgcccaa tagaaaggta tttcttttga gaaatcaatt ttgaaattta gagcattctg
    13681 tcttaaaagt tctttgtatc gttcattggc attaaaatac caattatttc ttgtggttaa
    13741 atcgccccat gctttctgaa tcagtgtagc tttcttatat tgactaggct gttttttttt
    13801 gccttttcca aaactttgct gaggcaaaaa gaaagtgcat aaaaagacaa ttatatatat
    13861 agattttcta atcaacattt ttagtttaaa acaatacgca atttccaaaa tttatttcac
    13921 ttaataaagc aatttgatat tattaataaa taataaaact aataaatgtt aaaaataaaa
    13981 tgctactgaa aatcaaattt ctatatttct gtttattcta ttatctttaa tacaaacaaa
    14041 tagatatgaa aaaaacgttc ctaattctat ttactatttc agtaggttta gtttcttgta
    14101 ccaataatca gaaaataaaa accgatcctt tagtagaagc aattgatacc accattgcag
    14161 cagatgatga cttttttatg tttgccaatg gaaaatggat aaaagaacat ccaattcctg
    14221 atgcttattc taattgggga attggaaatt tagtacaaga gcaaatttgg gaacaactca
    14281 aaaaaacaaa tgaaaatgct cagaaaagag caaacaatac tactgaacaa aaaataggcg
    14341 atttctatac gtctgctatg gataccaata caattgaaaa aaatggctta aaagatttac
    14401 aacctgaact cgatagaatt aataaaatct caaatttaaa agatttaaca gaagctgtag
    14461 cttattttaa taccattggg gttcctttat tctttgattt tggtatctat caagacttga
    14521 tgaacagcga taaaaatatt atttatttgt atcaaggtgg cttaggcttg cctaatcggg
    14581 attattattt caatactgac caaagaactt cagaaatccg aaaagaatat caaaataatt
    14641 ttatcaaaaa catatttacg ttctccacag ataaaacaac tgaatttaat ttttcagcag
    14701 atgctatttt taaattagag gaaaaaatgg caaaaagctc aagaaaattg gaagatttaa
    14761 gagatgctta cgccaattat aacaaaaagt caatacgaga tttacaaaaa ctaactccaa
    14821 attttgattg gaatctttat ttcaaagaat taaatatcca aaatatagag agtgtaattg
    14881 taggacagcc tgagtttttt gttgctttaa ataatttact acaagaacaa aatttgaaaa
    14941 cattaaagaa ttatatgcaa tatcatttaa ttgcaaacta tgctccatat ttgaataaag
    15001 caattgatga ggttgatttt aatttttatt ctaaattaat aaaaggaaaa aaagagcaat
    15061 taccacgatg gaaacgtgct ttgatttggg aagaagatgc tatgggtgag gaacttggta
    15121 aattatatgt taaagaaaat ttctcagaaa ctgcaaagaa aagatacatt gatattgtaa
    15181 aaaatgtgat gacagcttac gaaaaaagaa tcaaactatt gatttggatg agcgatagca
    15241 cgaaacaaaa agcgatagaa aaactacaca gtattactta caaagttggc tatccagata
    15301 aatggaaaga tttttcaaaa ttagaaattt caaaaaacag cttggtgcaa aatcaaaaaa
    15361 atatttctaa gttttggtta gatgatgcaa taaataaatt aggcaaacca gttgataaaa
    15421 gtatttggga aatgacacca caaacttata atgcctacta caatccaagt aataatgaga
    15481 tagttttgcc agccgcaatt tttactgttc ctggatataa ggattatcaa ctagatgatg
    15541 ctttagttta tggttatgca ggtgcatcta ccattggaca cgaaattaca cacggttttg
    15601 atgatgaagg taaagaattt gacagcaaag gcaacttaaa taaatggtgg acagcaacag
    15661 acgacaaaat gtttgaagaa aaaacaaaag cttatgtcaa tgtttttgat aatctgaaag
    15721 ttttagacag tttaaaagta aatggttctg ctacattagg agaaaatatt gcggatttag
    15781 gtggcattgc aattgcttta gacgctttta agcaaacaaa acagtataaa gaaaatcagt
    15841 taatagatgg ttatacacca ttacaaagat tctttttagg ttatgcttta ggctggagaa
    15901 tgcacatgcg taatgaagaa attgcgaata gagttttgac agatgttcat gcattatatt
    15961 tccatcgtgt aaatattcct ttctcgcata caccagaatt ttatgatgca tttaatatta
    16021 gacctgatag taaaatgtat gtaactcctg ataaacgggt aaaaatttgg tagaaaaaca
    16081 agaaataaaa tgtccaaatt gtggttctaa tcaaataaaa actgagtttg gtgaagtaag
    16141 ggtttgttta aattgtaatc ataaatttaa tgtacatctt gtacaaaaaa actcacccaa
    16201 aattatttta attggtattc tctttattgt tggattgatt gctattattc aatacactat
    16261 cccaaagttt gatgagcaaa aggctatgga agaattaatg caacaacaaa ctcaaacttc
    16321 tcaaaaaaac gaatcctcta agcgaaaaat tattactgaa aaaattgaat ttatccataa
    16381 taataaaatt tggaaaagta gaattgatgc aaattctgat ttaattacaa tttcacttca
    16441 aatcattgat gccgataata aaaaaattga cgatgaaatt gctttagatg taattcaagt
    16501 aaaagaagac aatccttatg atttagttac tgtaccactt gaaaaaagtt tatttgctat
    16561 cataaatcaa aataaaatta ttgaaattaa cattgcttca aaagaaattg ttgctaaaaa
    16621 caatgccttt attgctaaat ataaagaact aaaaaatggc atacagaaaa tagaatattc
    16681 caaagcgatg caagccatta tatttcaaaa tttagatggt gcaacattct attactttcc
    16741 taaaacacaa ctttttgtct cgaacataga attaactcaa ttgagaaaaa tggaacaaac
    16801 tggcgacaat tggaagtttg agaagaaata catcatttca aatggaaaat tatttgccat
    16861 tactaaaaaa gtacaacaat atacgatgga attatcaagc tctgatatcc aaacaatttt
    16921 ggaaggtgct gattggatga aaaaaaatta tgccatagtt gctattgagc ctaatcttaa
    16981 aaatgttgat tttaacaata cagaaatttt atacagcaac gaaactgaat ttgtttgtgt
    17041 aaatgagcca aaaaatgaag ttgtctatta taatctaaaa actaataaaa cttggcatag
    17101 aaaaattgat gatattcaaa acaaacaact ccaagcagct atttcacgca gtgaggtttt
    17161 gatttatcat tctaacaatg cttatgcttt tgataaaaaa gatggcaatt taatttggaa
    17221 atattaagct attcttttcc tttttgacga ttcagctcat catctagttc cttttccatt
    17281 gatttcaatg catcttcatt gatggtaata tccaaagtat tttcagcatc ttcagattca
    17341 gcagattttt gccttttcag ttcttttatt tctagacgtt tttgttttcg ttccgttttt
    17401 ttctgttcat aagcatcttt cttgattttt tttagtgctt ctctctccgc tttttcttct
    17461 ggtgttaatt ctccaataat tctcaattga tgtgatttta tttcaaaaac atcatcaaca
    17521 ggaatttcta ctacaaacca aaatacctta atttttgcta ctccaacact tctcatttta
    17581 attgacaaag tcaataactt agaaattaaa ttaaaaagag ctgtaatatt actaatctga
    17641 gtatgtgttg taaaaatagc agactgatga gcatttattt tctgcacttt attataatgc
    17701 aaagctccaa ttttaagttc tgctgtatta acaatatccg tttttatttc taaaacttta
    17761 ataggcaaaa agaacgaatt acttatttta atatctgttt ccaaatcaac ataagtcaaa
    17821 ttaattgctt ttgttcgaac ttcaagaata tcaatagttc tattctcaat tgctttcttt
    17881 acaataaaat caactatttt ttgtctaata ttttgaacaa atcccacaat ctttaagctt
    17941 ttctcaggtc aaagttaaaa aaaatgcatc taatccaact tagtatttca ttttatgata
    18001 atatttaacc ttagtataga aaaagcataa taattatttt tgtttaaaat tagttcttat
    18061 gttacaaact tcagatactt taaatagaat gtcggaatcg gcaactatca aaatggcaca
    18121 attaagtaga gaattagctg caaaaggtgt tcacgtaatt tctctttctc ttggagaacc
    18181 agattttcag acaccaaaac atataaaaga agccatgaaa actgctattg atagtggcaa
    18241 gtacgattca tattctccaa tttctggtta tgcagattta aaacaagcaa tttgcgataa
    18301 attaaagaga gataataact tagactataa accagaaaat atcgttgtat caacaggtgc
    18361 gaaacaatca ttagctaatg tttttgttgc ttttttaaat gaaggtgatg aagttattat
    18421 tccagcacca ttttgggttt cttatcctga acaagtaaaa atgtgtaatg ctactcctat
    18481 ttatatttct actaatgttg atgcagaatt taaaattacc gcagaacaat tagaaaatgc
    18541 aattacacct aaaactaaat ttttagtgta ttcatcgcca agcaatccta caggctctgt
    18601 ttatactaga gaagaaattg aaagttttgt gaaagtatta gaaagacatc ctcaagttta
    18661 tattgtttct gatgaaattt atgaacatat caattatgta ggcaaacacg tttcaattgc
    18721 agaatttcca agtattaaag atagagtaat ccttgtaaat ggacaagcaa aaggttatgc
    18781 tatgacaggt gcaagaatcg gttatattgc tgctcctgca tacattgcaa aagcttgcga
    18841 taaattacaa ggtcaaatta cttcaggtac aaattctatt gctcaaagag gtacaattga
    18901 agcacttttg ggcgaccaag aaccaaccta tgcaatgcgt gaagctttta agaaaagaag
    18961 agatttagtg ttgagtatat taaacacaat tccaaatata aaatgtaata ttccaactgg
    19021 agcattttat gttttccctg atgttagtgc ctatttcgga aaaacttttg aaggtgaagt
    19081 attgaaagat gctgatgatt tatgtttata tttattaaac gtaggtcatg ttgctgctgt
    19141 ttcaggaagt ggctttggag cacctgaatg tattcgattt tcttatgctg cttctgaaga
    19201 aaacttaaaa gaagcattta ctagagtaaa aaatgcatta gaaaaattag cttaggattt
    19261 actactttat tataaaaaaa atgagagctt aaaagctctc attttttatt tatttctccg
    19321 ctaagttttt cagttcagac aacatcgaaa agggcgtatc tactgctgcc gaattggcca
    19381 atcctgaagg aatattccca ccagtcaaac tataagcttg gtgtattact tcagttgaat
    19441 tacctttagg aataaacttc caatagcctt tcatttctgg tactcttaca taattatcat
    19501 ttataggtac tagatttggg gctgcttcca attcaactgt aacagtccca gtattgttgt
    19561 cttttataag tttataatga gttacgacat ctctatcttt tacaatcgga gcattgacta
    19621 ccatataagt ataaaaatca ttatcactaa tctttttaac aacttttaca cttcctgctt
    19681 gtgctttata caagaattga tcatgcaatt ttgtattttt aaaaattgac actaattcat
    19741 caactgtcgc attgattact gttacacctt tatattcctt tagactggaa tttggtcgct
    19801 tctttgtcca tacttttatg ccatttttat ctctttctaa agcccaatcg ttttgagcct
    19861 gtaagttccc taatatactc atcactaaaa cactaaatat aaaatacttc atctaattcg
    19921 tttttttaat ttgtatgcaa aatacgtacc atttttaatt tgtaaaagct tatatttttt
    19981 aataattttg tatttaactt tttttatatt aatatgaaaa catataaatt aatatttgga
    20041 tttttattgt tgattatttt gccaacatcg tttgctcaag gctatttggg tgttttggtt
    20101 aaagattatg acctctcagg aattaaaggt gctttaatcc ttaatatttt agatgatggt
    20161 gctgctaaaa atagtggact caaagagaat gatattatcg ctcaacttga taataatgca
    20221 attacaaata aatcagactt aatcaattta ttaaataaaa aaaattgggg tgataaaatt
    20281 catttacaat attatagaaa tggtagttta aatcatgtag atatcatttt gggatataaa
    20341 agaaatacaa aaacatataa tgttcataag aaaataatag acaaaaatca acattggatt
    20401 tttgaagatg ataataccgt tatagttatt gacaaaacca atcagccaat ctccattaat
    20461 aaaataggca acggttgttt attagatact tggtatatcc aacaagatta taaagacgat
    20521 gaaatacctc aatgttattt agatttggac gataaattat acactattga aagaattaaa
    20581 gccagtcaag aaaagagaaa agtaaccata gataatatca tttatataaa aaaaattact
    20641 gaaggagagc aatcaaaaag caaaactgaa gctatcgaaa agccaatttt agattttcat
    20701 acttttggaa tttcaccaaa tccaagtgat ggcttattta ccatatcaat agatacaaaa
    20761 tccaccacat cgtttggaat aaaaatattg aatgcttcgg gtctagccgt tcatgaaaca
    20821 aatgtatcaa gttctggagc cggaattata aagcaatttg atttaagaaa ccttgcaaaa
    20881 ggaatatatt taatacaatt agcggaagtt ggaaacgagc agaatgtaat cgcaaaaaaa
    20941 attatcttta aataaaataa attggctgaa attggttcga atattgagat agctgctgat
    21001 tggctaaaaa aagggaatgt tgtatctatt ccaacagaaa cagtttatgg attagctgca
    21061 aatgctttaa atgaaactgc tgttttacaa atttttgata taaaaaaacg cccttcgttt
    21121 gacccactga ttgtacatat ttcttccata aatgaaattg aaaaatatac cttatcattt
    21181 cctgaagtat taaaaccttt ggcagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21301 nnnnnattcc atttaatatt tataaattgt tttctttatt ttaaaataga tataagcaat
    21361 gtcgaaagaa gaagcctcgg aattaaagta tcaagctcca gaaacaaaat attttaaatc
    21421 aaaagaagaa tttaatgaag ccgtagctaa agattttata gaatatgcca accgttcatt
    21481 aaaaaaagga gaaaaatttc ttgttggaat gtctcatggg aaatcgccat ctggtgctta
    21541 caaatatgta gtaaagcatt ttgacaaaat catccatcca cacaatttgt attttacatt
    21601 tgtcaattct ccacttaaaa gtcaggaatc tgtagagtta gaaactatgg atgcacgctc
    21661 atttattaaa actttactaa aattaggatt gataaaaatc gaacaagttt taggaaaacg
    21721 atttaatgaa gcagaaattg aacaatatat gcttgaattt aatcaaaccg tccaaaaatt
    21781 cttagatgaa aacaataagg aaggtttaga ttatgccatg attacttcta atccaaaagg
    21841 aaggattgca ggcattgaac gaaaatcaaa agcctttgaa tccaacgaaa tcactactat
    21901 tgttacaatt gataaagaaa aagaattaac gtgcacacct agttttttat taaaaacaaa
    21961 acgtattgca ttcttagcta caaaagccga taaaagaaga cccttagcct ggttgtatag
    22021 caccgatgga caagcagatg aaagtcctag ttttattaga tttatcgata ctaataaaca
    22081 aaaaatcact gtttttgttg atgacaaagc actaacttgg ccacaaatcg agatttatag
    22141 agaaacacct tatggcgtta cgacgatacg tttagatact acttatccat ataaagaaaa
    22201 ttccaaagag aaattgccag ttttaattat ggtacatggt ttcttaggtt taaattcttt
    22261 tgatggctta ttgacaaaac tacctaccac aaaatatatt gcagctgcta tgcattatgg
    22321 tacaatacct gatgatttac ctgttaaaga atattccaat catgtaatgt tgaatattga
    22381 tgccgtaatt caatattttg gctcaaaagg acatcctgtt tatttatttg accattctat
    22441 gggtaatacc tatttcttaa tgatggatag agaatatgat aatttagtgg gtgtaaaaaa
    22501 atatctaaaa ggaagaattg gtgccaatcc cttctttggc gaagaagtaa aacatgctat
    22561 tttgggtttc atggattatg taatcattcc ctccatgaaa tttgtgaatg ctccaattga
    22621 aacttctttt ttagcgatgt tccgtagaat tgttccccta gatagtaaat ttggcgttcg
    22681 caaaagagct atgaatttag cgagtatggt aattgggaaa gaacatacag aaaatagtgc
    22741 gatttggagt gcgattaaag caagattgct atatcaaatg gggaatttag attctgttcc
    22801 tgaattaaat cgtattccaa ttgaaaaagc aatgaataga attccaccaa agatatttgc
    22861 aattcaaagt agttcagcat taagagaatc taaaaaattc gataaacagg taggcttaac
    22921 caatacatta aaacatcatc ttccagtaat gatacttaaa agcgagttag atggtgtagc
    22981 taaatatgtt ccaagattat atgaatcaga aaatgtaaaa gtagttgatg ttacgaatgg
    23041 ttctgaacat gattttttta gagagcattt ataccacatg gttgagccta ttcatacctc
    23101 acaattaatc agtgaattta ttgatgaaat agaagcaaat aataaaaaaa cttagattac
    23161 tctaaatatt gaagctttta atttttcttt gataaatggt ctaattatta tagttttata
    23221 ctttgatttc agatgaagca cgaacgttat tattataacc caaaaactct tcgatacgaa
    23281 aagtacaaac ttacagttgt ccagcgagtc ataagagtta ttggattttt tacttctgct
    23341 ttcttttttg gctttttagc aatgatattg ggttacaatt tttttgaaac accacaacaa
    23401 aaaacagata gcttaaaaat aaaatctatt caagataggt acgatatttt aggaaaaagg
    23461 gttgatgcat tagcttcagt aatcaatgaa ttgggaaaaa aagacgacaa tatttataga
    23521 gcaatttttg aggcagatcc tttacctaac gaattaagac aaaagcaaat caatcgttct
    23581 aaagcgtatg atgagttaaa aaacatacct gaaggtaaaa ttgtagcaca aacattggca
    23641 aaaattgaag atttagaaaa gaaaatttat attcaatcta aatcgtacag tgaacttacc
    23701 caattaatca ataataaaga gaaattattg gctgccttcc tgctattcag cctgttgcca
    23761 acaaagactt aaaacgtatt gcttcaggtt ttggttatcg tatagatccc gtttataaaa
    23821 ctacaagatt tcatgctggt ttagattttt ctgctcctac tggaacgcct gtttatacta
    23881 ctggcgatgg tgtcgtggag aaagctggat cggaaggtga tggctatgga tataaagtta
    23941 ttataaaaca tggttatggt tatagtactt tgtatggaca taatagtaag gtcttagtaa
    24001 aagttggaca agttgtaaaa cgtggagaaa cgattgcatt ggtgggtagt acaggaaaaa
    24061 gcactggtcc acattgtcat tatgaagtat ggaaaaatgg tcaaaaaatt aatccaatca
    24121 acttcttctt taatgattta tcgccaacac aatacgatga aattctaaaa attgcagaaa
    24181 ctagcaatca atcttttgat taaaaaaact atcgaaattt attgataagc tattatcaaa
    24241 actaaaattt atattttatt aacagcataa aagtacttta atacttttat ataaaaattt
    24301 gtatgtatat ttgtattgaa atttgataat taatctttaa attaagcaat atgcaaacaa
    24361 taaatgcacc aataatcaat gttcaagaaa tagaaccaaa gcttcgtcat ctaactattt
    24421 tccaaacatt tgacaaacta aatcctggag agtatttgat tatccataat aatcatgacc
    24481 caaaacctgt tttttatcaa ttacaagaaa ttagaggcaa tgtttttgat tgggtatatt
    24541 tacaagctgg tccagaatgg tgggatatca aagttactaa acatgaaaac gctccaaaac
    24601 ctgctgttat tattaatgaa aaaggggaaa ttgaaattac tgtacctgct ttagaaccaa
    24661 gattaaaaca cgaaacgata ttttttgcat tcgaaaaatt aggttctggt aaaactatga
    24721 taattcataa cgaccatgac ccaaaacctg tttattacca attacaaagt atgcacggcg
    24781 atatttttac ttgggattat attacagaag gtccagaatg gtggcaaata agagtaacca
    24841 aaaaagcatc tgctcaacca aatccatata tcaatgacaa aggtgaagtt gtaattaatg
    24901 ttcctgcttt agaaccaagt caaaagcacc caacaatatt taatactttt gagcgattaa
    24961 ttcctggaaa aacattagtt atccataatg accatgatcc caaaccattg cactatcatt
    25021 tgcaaaatat gcatggggat actttttctt gggaatattt acaagaaggt ccagaatggt
    25081 gggatgttaa aattataaaa aaacagaaag aagaaaatac tagcgaacca aactatctaa
    25141 aagttggaaa aaatccatct aatcaaaagg aaacgactat cactattcca ttgatagaac
    25201 ctaaacttaa acacgctact attttcaaaa tctttgatac attagaagtt ggagaaagtt
    25261 tagttatcca taatgatcac gacccaaaac ctgtttatta tcaattatta ggagaacgtg
    25321 gagagacttt cacttgggag tatttggaac aaggaccaca atggtgggat attagagtta
    25381 ctaagaaagg cactgaaaat gatgaaacta ttggcgaaat tgttgcaaag gacattagaa
    25441 aagctgaagt actcaaaaaa tttggaatag acttctgttg tggcggaaat aaaacagtcc
    25501 gacaagtatg tactgaaaaa ggaattgatg ccaatttagt tgaacaagca ttgcaacagc
    25561 cgattcaaaa tctaactaga aataatcata attataatga atggaatatt gatttcttag
    25621 cagattatat tgtaaatgta caccatagct atgtaaaaaa atatctacca gaaatagttg
    25681 gatatgctgc aaaagttgca aaagtacacg gaccaaatca tgcagaatta gcatcaatta
    25741 atgacttggt ggcaaaaata aatactgaat tattaaaaca tttgaaagaa gaagaaggtg
    25801 tgttattccc tttgataaaa aaaatagtac aagctaagca ttcaaataca aaattagata
    25861 gtacaaataa ttttgcacaa gttttagaag aactagaaaa agaacacgaa caagttggcg
    25921 agatggtaga taaaattaga atattaagta atgaatttgc tattcctgaa gacgcttgca
    25981 ctagttataa attattgtac aaaatgctac aagattttga agatgactta catattcaca
    26041 ttcacttaga aaataacatt ctttttccaa aatcaatagc aattgaaaaa gaattagttt
    26101 aaaatattat tcaatttaaa ataatattat gaattttaca gaagtgcata agaatgaaaa
    26161 ttataaagta acagaagttt tcttggaact cggcgaaaaa atgcctttac atcaagcaac
    26221 atctaatgcg tttgtaattt gtagaaatgg aaaaagtaaa ctgacatttt ttgataaaac
    26281 tttagtgctt agtcaaggcg aatccatatt aattcaagct aacgacccac acgaattgga
    26341 agtattggaa aattttagtg catcaattat tatggatcca gatgctaaaa tagatttttt
    26401 gtagtattat agtagaatga ttgctaagca ataatacttt tatttatgtt aattaatgaa
    26461 caaaccaaaa tagccacatt gataaagcat cacccagatg ctttagaaac tatcgtaact
    26521 ttaagtccag atttcaaaaa attacgtaat ccaatcttgc gtaagttaat ggcaggaaga
    26581 acaagtattg caatggcatc taaaattggc ggatgcaaac ctgaagattt tttcaaagct
    26641 cttcaaccat taggttttga agttgataca aaaggagtaa ttccaaaaga aaaagaacct
    26701 gaacaaaaag cacttcctat ctatttaaaa aatgcaaaag cagaacaaat tgttaatttt
    26761 gatgttcgtg aaatgttggc tggtggcagt gatccattac gagatattca gcttaaagta
    26821 aaagatttaa aagctggaga aatactaaaa ataatcaata cttttgagcc tgtaccttta
    26881 gtaaaactat tagagaaaca aggtttccaa acttatgttg atgcgatttc agacaacctt
    26941 tatgaaactt atttttttaa aacagatggg acagattcat cagcagattt accaactgaa
    27001 attcaaaact caacagattg ggaacaatta ttacaacatt ataaagataa attaagagag
    27061 atagatgttc ggcatttgga aatgcctatg cctatgatga cgattttgga aaacttagaa
    27121 actttacctg aggatatggc gttatatgta aatcataaaa gaatacccgt atttctttta
    27181 acagaattaa aagatagaaa ctttgatttt agaatcaaaa atattcaaga tggtgaagta
    27241 tatttattaa tctttaaaaa taaatagttt tgattggtag tttagttggt aatggcttac
    27301 aacaaacaac atcttataaa gttgtaatac ctttttatgt ttatgcagca gtttcattat
    27361 taattggaac tatattacta ttctttcata ctgatattgc acaccaacac tattttcatc
    27421 cttatacact tgctattacg cacacaatgg cattaggttg gggaactatg attatttttg
    27481 gagcatcaca tcaattattg cctgttttag tggaaggaaa attagatagt aatttattgg
    27541 cttatctaac atttattttt gcagctatag gcattccatt tttagtagct ggattttata
    27601 tgtttcaaat gggttggttg gtacagcttg gtgctatttt aataaatatt gccgtgattt
    27661 gttacctcac caatgttata atgagtattt ataatagcaa aaagtataat attcatgctt
    27721 ggtttatagc aacagcgtca ttgtggttat tttcaaccac tttctttggc ttactattag
    27781 ttttcaattt tacacgttcc attttaccaa aagattctgt cgcttactta acaatacacg
    27841 cacatttagg tatagtaggt tggtttttat tattagtaat tggcgttggt tcacgcttaa
    27901 ttccgatgtt cctaatttca aaatatacca atgataaaac attatggcaa atctttattt
    27961 taatcaattt gagcttagta ggttttatca tttttaaatt aatgaactgt cctacaactt
    28021 attattatat tgtcttggca cttggattga ttggtattgc attatttgga aatttttgtc
    28081 gaatggcaca caaagtaaga attcggaaaa gtgtagatga acaaatgaaa gtttccctct
    28141 tatctgtatt gcaaatgtta tttccaataa ttgctttgct cattgtcatc atacttttcc
    28201 cttcaaaaaa atatgctgat atgtcaattt tatatgggtt ttgcatattt tttggttggc
    28261 taacagctat tattttggga atgactttta aaactttacc atttattgtt tggaataaag
    28321 tatatagtaa gaaagcacac gccggcagaa caccagttcc caaagattta ttcaatgaaa
    28381 atatttttaa cggaatgact ttggcttata ttttaggatt tatattattt ataacaggaa
    28441 tgattcttag cctaaatatt gttttaaaaa tagcaacaat agcattagtt ttagcagcta
    28501 gtctatatgt ttcaaatata actattattt tattgcataa accgaatatt aaatgaatga
    28561 agtaacaaac gacagtataa aattaaacat tgccttatct gatttgtata atgtttatga
    28621 tccagaaatt ggcttaaatg tagttgattt aggattgata tataaaatta tctttgaaga
    28681 agtaacgcaa cgtttagatt gtgagatgac attgaccacc caattttgtc caatgggaga
    28741 aagtattaca aatgatgtta tccaatcact gacaaaatca tttcctagtt gggatatcaa
    28801 tgtaaatctt acttttgaac ctgcttggga ttataccaaa atttcgccag aagggcaaga
    28861 attcttaggt cattaattat gttgagtcta acttgtaaaa cagccataaa agcagtcatc
    28921 tatctttcca gtaaaagtgt aaatggagag aaagttgggc taaaagaaat tgcggaacac
    28981 atcaatgcaa gtgagcatac tgtgggcaaa acgctacaaa ctttagtgcg tcatggcatt
    29041 atcaatagtt taaagggacc aactggtgga ttttatatca caaaagcaca acaaaatcag
    29101 ccgatatcga atatcgtaga aaccatagaa ggtaaagaaa tttttaaaga atgtggtttg
    29161 ggtttaagca aatgttctgc tcaacatcct tgtccaatac acaacgagta taaagtagct
    29221 cgtgaactca tagaacaatt atttagagag aaaaaagtaa aagacctttg cgatccagtt
    29281 accaacggaa tagcatattt ggttggataa taattcataa aagatatttt tgtatgggag
    29341 aaaaaagtaa tcaatcaaca cctttgcgtc cacaaggaga aagagtttta agttctaatc
    29401 ttgtagaaat ggatgtcaat cagtttattc agcagattaa atcagaaaaa gtttgggcag
    29461 aaagcgacca caattcaatt acaattttta aatctgacaa aatgcgaatt gtactgattg
    29521 gtatgcatga aggagcagct ttaaaaacgc atactgctaa tgctattata agtgttcaag
    29581 tggttgaagg ctcaataaaa tttacagcag atgaacaaac cattgcttta gaaaaaggga
    29641 aaatgatagc tttacaagaa aagattcctc atagtgtatt cgctattgaa gatagtttct
    29701 ttttattgac cttagctatg taattttatt tcttgcaata attctgcaag aaataaaata
    29761 aactataaat tacaaagcaa tcaacatttt ttctggattc tctaaataag attttacagt
    29821 agccaagaaa ctaacactat cttttccatc aataattcta tggtcataag ataatgccaa
    29881 atacatcatt ggtctaatct caatatttcc attaataacc actggacgct catctactct
    29941 gtgcattccc aaaatagcgc tttgtggttg attgataatt ggcgtactca ttaatgaacc
    30001 aaaaacgcca ccatttgtaa tcgtaaaggt gcctccttcc atatcttgca tacttaattc
    30061 attattacga gctaaaacag ccaagcgttt aatttctctt tctattccag caaagctcaa
    30121 tgtttcagca tttctaatta ctggaacaac caagcctttt tctgtagaaa ccgcaataga
    30181 aacatcgcaa taatcgtgat aaataatatt ttcgtctgtg atgtatgcgt tcactgctgg
    30241 gaatttttgt aatgccaagc aaactgctct tgtaaagaaa gacataaagc ctaaaccaac
    30301 accgttttgt tcataaaatg catctttata tttcgctcta atttcattga tggcagacat
    30361 atctacatca ttgaatgtgg ttagcattgc cgtttggttt ttcacctcca ccaatcttct
    30421 ggaaatagtt tttcttattc tagtaagttt ttcaattcgt tcttctctta atccaactgc
    30481 tgttggagca actattgcag gtgatgtagg ttttgttgaa gcaggtacgt ttgttgctga
    30541 ttttggagct tctgctttct gagcatcttc ttttgtaatt cgaccatcaa caccacttcc
    30601 tttaatatct gatgctggaa tatttttttc atctaatatt ttctttgcag ctggcgatgg
    30661 agtgccactt gcatagcttg tattattcgt agtttgttga atattagcac ttacttttat
    30721 atcctcagtc tttgcaactt caggtttctc cgaattacct gcagtcgatt gcggtttggc
    30781 tgcatctgtt tcaatataag caacaacagc accaatttga atatcatctc cttcattagc
    30841 tgcacgagta attacgcctg caaactcagc tggcaattca aacgtagctt tatctgattc
    30901 aaactctgca ataggttggt ctaattctac atagcttcct tcttctacta accatgatgc
    30961 taaggttact tcactaatag attcgccaat tgaaggtact tttatttcct gtttcatata
    31021 ttgtaaatta ttgtgtcaat agatataaat acaaatttat ccttattttg ttttaaaaga
    31081 aaataaagct atttattttt ttcagatttt gccatataat gtatcattcc atcgcctaaa
    31141 cctactttcg gtacgattaa attttcgata gttgcttttt ccattataaa attaaaaatt
    31201 tttgcagcag gaacaatcac atcagcccta tctggtttta attgatattg gataattcgt
    31261 tcttcaatac tcaacttttc caatttatgt atcgtagttt ctatagtatg cttagttgca
    31321 atggtttttt tggaattcgc aaataatttc tgaattgaat taatatttcc tccaactcca
    31381 ataccttgaa tattactata tgtcgaattg atttctaaga gccagttttc taatttctcc
    31441 cattgcttat cttttacttt tcccatacac atacgaagcg taccaatatc aaatgaacgc
    31501 ttagcaattg tttttcctcc acttaaaaga ataatttcag tactgccgcc tccgacatca
    31561 atatacaagt aattggtatg ctctttaaaa tcatacaatt ccacatggtt agacagcaca
    31621 aattcagctt ctctctgccc tgaaataatt tcaatttgaa tacctgtctt tttatgaacc
    31681 aaatcaacaa tttcttgact attgcttgca tcacgcatcg ccgaagttgc acaagccata
    31741 acctttctag gcttatgaac atctataaga tttttaaatg ctttcatcgt tgaaagcata
    31801 tcttcaattt tgcttttaga aaacgaacct cttgtaaatg attcttcgcc caaacgcaaa
    31861 gctactctat acatcgcatc tttaatatac tgtttgcctt ctggcgtatg atatacgttt
    31921 gtaaaaatta aacgtacagc atttgaacca atatctattg cagcatattt catttatagt
    31981 tacgattaaa attcaagatt gattctatct aaattttcgc aacaatgttt gagcaataaa
    32041 acagagtttg aaatatcttc tttatgtgcc atttctatga cttggtgcaa atgtctagtc
    32101 ggaacagaaa ttgctcctgc tattgctcca gttttaccca ttctttgtat tcctgccgta
    32161 tcggtaccac ccattggcaa taattcagac tgccatttta tttgaaattc ttcagcaatt
    32221 tgtttttgaa aacgtaccat tctataatca caaatagtgc tagcatccat gattttaata
    32281 gcaacacctt ctcctaactt tgtaatttgt tcttcaggtc ttgctcctgg caaatcataa
    32341 gcaatcgtgg tatctaaacc aaacccaaaa gtagggttaa tattgtgtgc agcaacattt
    32401 gcacctcgaa ttccaacttc ttcttgaact gtaaatacac catatacatc gtaaggacaa
    32461 tcttttaaag cttttaatgt ttcaatcaag atataaactg ccactctatt atctattgat
    32521 ttacaattca cacaattgcc catttcaacc aaagtacgct ctctagtaat tgggtctcca
    32581 acctgaacca aagcttttac ttcatcagca ggcataccta aatcaataaa ataatcagaa
    32641 atttgtgcta ctttatttct ttcctcaggt gtcataatat gaataggttt gctactcatt
    32701 acacccaata aatctttttt accatgtaca atcactcttt gggcagtcaa tgttttggga
    32761 tcaaaaccgc ctaaagtatg aaatcgtata aatccattat cgtcaatata ttttactata
    32821 aagccaattt catccatgtg agcaccaatc atcacacgtt tactgctatc ttttcctttt
    32881 tttatagcat atacattccc taaattatct gtttctattg ttaaatcttc agattttagt
    32941 tcttcataaa ctatttttct tacttcgttt tcaaaccctg aagttcctgg cacttcacat
    33001 attttggcta ataatggaat attaacttgc atatatatta taatttttta tcaaaattac
    33061 atgtatttct gataaaataa agaaattaag tctaaaatta tgatttatta gaataaagca
    33121 atcaaaatat atacaaaata agaacaggca tttctttcga aatgcctgtt cttataaaac
    33181 aaatgaaaac aactctatta agcaattgct tgttcgtatc ttgctgctac ttcagcccaa
    33241 ttaattacat tgaaaaatgc attaacataa tctggtcttc tattttgata gtgcaaataa
    33301 taagcatgct cccaaacatc aattcctaaa attggtgttc ctttgcaatc tgcaacatcc
    33361 attaatggat tatcttgatt tggtgtagat gtaacacata attgcttatc tgcagtaact
    33421 gccaaccatg cccaacctga gccaaaacgt gttgcagcag ccttagcaaa agcatcttta
    33481 aaagcttcaa atgagccaaa tgcagcatta attgcatctg ctagttttcc agtaggttct
    33541 cctcctccat ttggagacat tactttccaa aacaaatcgt gattaaaatg tccgccacca
    33601 ttatttctaa ctgctggtga taatcttgaa acgtttgcta ataattcttc aatagatttc
    33661 ccttcgttct ctgtaccttg tatagttgca tttagattgt ttacataagc tgcatgatgt
    33721 tttccatgat ggatttccat tgtttgcgtg tcaatgtttg gttctaatgc attgtgtgca
    33781 taaggtaatt gtggtaattc gaatgccatg atttataaat tttaaatgtt aaatatttag
    33841 attcacaaat ataacaatat tgaccacttt ttgtttaatt atatggtgga ttcaacttat
    33901 aaatgtaaaa aattaagaat ttttcacttg taataaaaat aaatgatgtt taatattgca
    33961 ttacaaacaa tccataaaaa taaaacaata ctataaaaaa aacctttgta ctacttgcac
    34021 ttttgctatt cgttatttca tttgcttaca cacaggatat tgtaaacttg aactttaagt
    34081 atcaattttt tgattcaaga gaaggctttg ttaaactgag caaattggta attaaagcaa
    34141 atggcaaaaa gattggggaa agtcctgcaa aactagagaa tattgaaaat aaagtttctg
    34201 ttcagctacc taaaggaaat tatgagatag atgcaactgt ctattcaatg tataatggag
    34261 tttgggaaga acgtaccgaa gcgaatgatt atactatgga ttgtaagtat ataatagatt
    34321 ctttattgct tgaatcagat aataatataa cgcttacatt taatgcagta acaggttaca
    34381 aaaa
//
LOCUS       DAOQCW010000011        33741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_464_length_33741_cov_5.609511, whole genome shotgun sequence.
ACCESSION   DAOQCW010000011 DAOQCW010000000
VERSION     DAOQCW010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 33741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33741
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_464_length_33741_cov_5.609511"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="PLS16_01985"
     CDS             complement(<1..650)
                     /locus_tag="PLS16_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59875.1"
                     /translation="MTESKYQINMTENLYFEYRKWCFFSGLTLDQKEEVQSVMNEFNA
                     KGWKVRDFEWSGFPKLSFFKMLKVIIITTITLGFCSYWVGFCVIFESTLNNSTTDINA
                     QLPQTDIKTESLNADSELDILAKLKSLNVITESEYSEKVESLEINKTLSENLNKITDL
                     LKTAKAKGVITETEYIDKVNKVLLEEKAKIKEWLSRKPTINDIPAEIVNELPEHLR"
     gene            complement(712..1161)
                     /locus_tag="PLS16_01990"
     CDS             complement(712..1161)
                     /locus_tag="PLS16_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59876.1"
                     /translation="MSTVIESQDPNLQPKPSIFHKGKKATVSFFKKLFLILFAFLIIG
                     IVGFFAWANFTRSSGSRAGVLITFNQEGYLFKTYEGELNLGGINALPGNTIANNMWKF
                     SVRDANVASKLDTLQGKKVRLQYKEKAKNFFWQGNTNFFVDGVEVIP"
     gene            1267..2565
                     /gene="ricT"
                     /locus_tag="PLS16_01995"
     CDS             1267..2565
                     /gene="ricT"
                     /locus_tag="PLS16_01995"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNJ59877.1"
                     /translation="MGCSGCGSSTPNGCKNNGHCASGGCSKLEVYDWLADISIDNFST
                     YNIFEISFKNGSRKGFYKLEKPLEVFVGDYVVVEATQGYDIGRINLKGELVKLQMRKK
                     KINEKTYEFKQVLRIAKDHELELMDKVRDLEKETMLKARVLARNLGLDMKLGAVEYQA
                     DGKKATFYYFADGRVDFRELIKVLASEFKIKIEMRQVGARQEAAIVGGIGSCGRELCC
                     STWMTSFKSVNTTAARYQNLSINQTKLSGQCGRLKCCLNFELDTYLDALRDMPQKAEF
                     IESEVGVAKLIKTDILKGLMTYIEKETNKAYTLTFENAKSVLEMNKAGKKPVNLTDLA
                     EAPEEQNDNAEFVELVGQISLSSLERSDKKRKQKARQDNNKQRNKPNPNASNKQEGQT
                     IAKNEDKKNTPTENSEPKPQKSNNRPNHHRRNRKPGDNKQ"
     gene            2811..4640
                     /gene="mutL"
                     /locus_tag="PLS16_02000"
     CDS             2811..4640
                     /gene="mutL"
                     /locus_tag="PLS16_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNJ59878.1"
                     /translation="MSEKIKLLPDAIANQIAAGEVIQRPASIVKELLENSVDAGAKNI
                     KLIVKDAGRALVQVIDDGSGMSEIDARMCFERHATSKISNINDLFSLHTMGFRGEAMA
                     SIAAVAHVELKTKMQDKNVGTCVIIEGSKIILQEPCATNDGTSISVKNLFYNVPARRN
                     FLKSNQIETKHIIDEFLHVALANPSIFFSLHNNGSEIYHLKQGNLRQRIVSVFGKNHN
                     DKLVPIEENTEYVSVYGFIGKPELAKKTRGEQFFFVNNRFIKSNLFNHAIQSAYENLI
                     QDKTYPFYCLFIDILPSAIDINVHPNKQEIKFEDEKSVYALLRASAKYALAQYSIMPT
                     LDFEHQANINFLTNPQSSAENEFKTLGSRMNKTEMNQQLHSEHYEVRHAPAFDNWEKL
                     YQLDAETEQKIITLQSKLDYEDDEVLETKVTFKPIQIHQKYILMQVKKGFILIDQNRA
                     HQRILFEQYLERLETKESSSQQTLFPRTINLSAGDYQYMEEILPDLRKIGLDIEPFGN
                     NSIIVHGLPPELVNEDEHSLIENILQHYKESLQTDNLSQHQQIAKSVAYHTAIKPSTI
                     LDEKSMQNIIDSLFACKQPNINPVGLKTFTTYTIEDIQLLFDK"
     gene            4704..5390
                     /locus_tag="PLS16_02005"
     CDS             4704..5390
                     /locus_tag="PLS16_02005"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNJ59879.1"
                     /translation="MTQFGRSGFLQNLTPIVKNIILINIIVFVIQFIFPQVTDLLAMH
                     YPSNPAFKPYQLFTHLFAHGGFMHILFNMYALFLFGSVLERIMSPKRFIQLYFISGFG
                     AIAFHLLISAIMAYKTAGTVMITYEMAEVNKTLSEIYFPGLVGASGALYGLMAAFGIL
                     FPNTPLMFMFVPVPIKAKYMIPLVILIYDIGFAQYGLDNVAHYAHIGGAIFGAIVTIY
                     WRKTDRSSFY"
     gene            complement(5387..6886)
                     /locus_tag="PLS16_02010"
     CDS             complement(5387..6886)
                     /locus_tag="PLS16_02010"
                     /inference="COORDINATES: protein motif:HMM:NF016946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HNJ59880.1"
                     /translation="MNKYIKSIEYLRLDERSVAIFRIVLGISILYSLIILKLPYTVEF
                     WGEHTIIPINILQKANGIGSWSIFDYVRNDGFAYFWMSVSIVLAVLFTIGWQTKIVSF
                     LLLFFFFNILQAYTRYNTGFDKYTFQLLTWACFLPLSNFFSIKNNTQKIKTPLWVSVI
                     VIVQIAMVYFITGLAKTGDAWKNGYAVQVLTSDLWMRYTTAEIFRDNAWLYMPLTYAT
                     LLLDYLFPFFLFIKFKNNVLRYFAIIYLLGFHISIFSLVDVANFSITGVAAAMIIIPS
                     SFWEKLKIQQGFKFYSTIKSNSQKMMIYGFTIFVLYTIVQKNLLFYTRLDVYKTKHES
                     IAYKFFNAINIPTVVQNSFQFQYWKMYAPSPASEGGWLSIEYKGEDGFLYDYFTKGLL
                     SNTEHKISFRPKGMEAFLLFNGRSFKYEDRWYSAIFMRYWLSYQLKKNKIPVSSYNNY
                     YLAVYSLDVSQQDADLSQVHKEIIPLDVLETFVKPYEQNLLKKNNEETN"
     gene            6971..8929
                     /locus_tag="PLS16_02015"
     CDS             6971..8929
                     /locus_tag="PLS16_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59881.1"
                     /translation="MIFSKRNIIYYLLFTFITFVFYSSAIKNGYSLDDQYIFNNIPAE
                     GSSFKDIFSVFAKRFDISDYRPIAMFTFALEQFIQGSINPKVSHIINLLIYVILCCTI
                     FYVLQRLPIKNAIPIALVTTLLFIVHPVHAGVVNNLKSRDGLFSMLFIVLALNSYINY
                     EVTKKRFYFLFIALFFVLGIYSKRDAYNLIFILPLLAFFVYNRSLKKDILLYFVGLFM
                     LIILSTSIIDLLIPFNAKVFEKSFMITENPLIADNTFFNRLGMSVATYLYYLKFMVIP
                     KGYYFYFGFNMIPLRSILNPITLVQLVVVILPLLAAFIMYKKNKHFTLGILIFYSSLL
                     YCANFVTEVQGIVADRYVFIASLGFIFAFAVLIVDILPSKIEKQIANLSKTIQAKHIP
                     IVITILIMLLYFPFVKARNNAWFSKESLFEADMPHLQQSFEANRIASTHYAQVGMNSR
                     NPSEQESNFMKALQYAKQANALNSDDIHTQETEGIAYYGLGKTAEAEKQFLKIIKKFD
                     TSVVSWDLMGDMTFKRQNYDSATLCYANVYRLEKSNESVYYKYPNALVQNGKRDSAFN
                     FLEKLSKTYPDWYVPYESAAYLYYQNGDTYYGHSFLVVALEKGLNNRQTYERTKNEII
                     QGTQNPDVKISTAYKQLLVRIEKIPIPN"
     gene            complement(8937..10463)
                     /locus_tag="PLS16_02020"
     CDS             complement(8937..10463)
                     /locus_tag="PLS16_02020"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNJ59882.1"
                     /translation="MAQATDSLQKIVAHCKEYGFIFPSSEIYDNLGAVYDYGPFGVEL
                     KNAIKEYWWKSMVQLHENIVGIDASIFMHPTTWKASGHVDSFSDPLIDNKDSKKRYRA
                     DVLLEEKIDKFKEKGQKELEKKGIDKNATEEDKAKASRDYAKGVELEKAYTEAAQASD
                     FARYKALIEEYEIVCEISGTKNWTDVRQFNLMFGTQLGSVAEESNTIYLRPETAQGIF
                     VNFLNVQKSARLKIPFGIAQIGKAFRNEIVARQFIFRMREFEQMEMQFFIKPGTQKEW
                     FAKWKETRLNWHLALGTNPESLRFHVHEKLAHYADAAEDIQFRFPIGFKELEGIHSRT
                     DFDLGNHQQYSGKKMQYFDSEINENYVPYVVETSIGLDRMFLRVMSENLKEETLENGE
                     TRTVLSLPPALAPIKVAILPLTKKDGLPEKAREIEAKLRFVGRTFYEEKDTIGKRYRR
                     MDAIGTPYCITIDHQTLTDNTVTLRDRDTMQQERISIDNLEQFIADKVNMNTLLRKLV
                     "
     gene            complement(10496..11572)
                     /gene="gcvT"
                     /locus_tag="PLS16_02025"
     CDS             complement(10496..11572)
                     /gene="gcvT"
                     /locus_tag="PLS16_02025"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNJ59883.1"
                     /translation="MKTTALTDTHIALGAKMVDFAGFNMPVRYTSDIEEHNIVRNGVG
                     VFDVSHMGEFIVKGVEALPFLQKIISNDISTLEIGKAMYGYLPNETGGIVDDLLVYKM
                     ADEEYMCVVNAGNIDKDWAWFTQHNTNNADLKNISDNIALLAIQGPKATEALQCLTDV
                     DLNSIPYYHFKIGKFAGIDNIIISATGYTGAGGFEIYFDKSNAATIWNQIFEAGKPQN
                     IQPIGLGARDTLRLEKGFCLYGNDIDDTTSPLEAGLGWVTKFNKDFIAKDILLKQKEN
                     GISKKLVGFKMIDRGIPRSHYLIKDADNNEIGHVTSGTQSPTLGYGIGLGYIDLAHSK
                     VGTEIFIEVRNKLIKAVVTKIPFL"
     gene            11841..14270
                     /locus_tag="PLS16_02030"
     CDS             11841..14270
                     /locus_tag="PLS16_02030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HNJ59884.1"
                     /translation="MNRLKLLFVALSLNGLVYAQMADLKIKSIDGDKNGITLFQASLI
                     EADKDTVANVDGVALLSNIPYGSYQLEVLSPGYQPYTTTVNLNEEEQDLTVTLTTDTI
                     LNLDNVEIFGERNDKPKGLEMITRLPINPNDNIQTVSVISYKVIEAQGALTITDAVRN
                     IPGVTLFGSYGGVKESMSTRGFRGVPVLKNGIRMDSQFQTASGVVDMQGVESIQMIKG
                     SAAITQGVITDIGNAGGVINVVTKTPNFTNLGNVSLRYGSWHQVRPSFDIQRVLTQKK
                     NIGIRFNGVYEYGKTFREGTSAHRFYFNPSFAWKVSEKTSFIIEGDYFNDNRTPKNDA
                     VNLAPGQGENALYIIPKDKFLGFNTDNNNTKMSSFMARFDHNINDKLMIRAAYGFSTY
                     QVDNISTSSNAAFKNKDFNLRKRDVTKSLRDDKNSTLQFDFIAQNIKTGPFKHTIQTG
                     FDYRSANATTTSFDAVTIDTIDIYGDFTNDLSKVVKYDNMGNEKLGVNVVFNPKTPVV
                     STYHTFGILAQDIIELHKYLKLFIGVRYSEIYTKDPLGTKGKRQSAWNPSVGLMLSPT
                     ENINIFGSYTNSTSLRSASNLMSNGQEIGPSRTQQFEAGIKTAWFKDRLRYNLTYFHI
                     YTANLSNSEYVEGTNVTTGYYFKAGDLLRDGIETELNGRITKDLTVMVGYAFLKARYK
                     NSPSYVNGSAPMNAPTHTANGWLQYQFSQGALRGLSLSAGVYYVGKRPVNEYSLTPDG
                     HGNMGGEKPFDMPAYTTLNAQIGYTYKAFSARLYMNNLTNKIGLNSYFRGGYINQTEP
                     INASLSISYKF"
     gene            14346..15536
                     /locus_tag="PLS16_02035"
     CDS             14346..15536
                     /locus_tag="PLS16_02035"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HNJ59885.1"
                     /translation="MKKKEIYTFKKLIGDLHLWLGVASGIILFVICFTGTIYVFKTEI
                     EEYLEPHKYNIEAQQQILPIEILIANTEKETKGKVSRIAYTDKQTRPYEINVKTNPED
                     RRGTNFMVNQYTGKVIGSVKGPATEFFFTMFKLHRWLLLDMEKGRPITGAATLIFTFL
                     CLSGFILWLPKKIKGLRSFKPGLTIMWRARWKRINHDLHNVLGFYSLIFLLIMSLTGL
                     TWSYEWYNNGLYKLLTGKEMEQKGGPGGGKGNKGEKGKKDNKSIEAQESIIIPTITYE
                     KALDIANTKLAYQGKTIINKIAQDGSPYEVIKFNKDRFNEEAADKLTINATSGNIEKV
                     ELFDDLSLGERVMKQIRGLHVGTTYGMWSKVIYFIVCLIATSLPITGIIIWINKMKFG
                     SSTD"
     gene            complement(15539..16060)
                     /locus_tag="PLS16_02040"
     CDS             complement(15539..16060)
                     /locus_tag="PLS16_02040"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ59886.1"
                     /translation="MIRIAITGPESTGKTTLSKTLAQNFNCHWVPEFARIFLENTNGY
                     YTFEDLDLIAKGQLEAWQRHTNDELCFYDTEMLVMKIWSSFKFGKVSDFIHQAYQYQA
                     IDLFLLCKPDIDWEEDELREHPEKRDELFELYFNALSENKIPFKIIEGNYDERIQLAI
                     KIVSDFLHKSIKK"
     gene            complement(16057..16662)
                     /gene="pnuC"
                     /locus_tag="PLS16_02045"
     CDS             complement(16057..16662)
                     /gene="pnuC"
                     /locus_tag="PLS16_02045"
                     /inference="COORDINATES: protein motif:HMM:NF016837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HNJ59887.1"
                     /translation="MNDFLSSFFQGIYATSYWEWQAIICGILYLYFAIKGKVICWIFG
                     FINAATYVYVCADAKLYLDAILQVFYVIMAIVGYLNWNKNDTTQIHTWNLKQHFFIII
                     LTTICSLILGFIFKKYTLQASPFLDALSTCFSLVTTYMVARKVLENWIYWIVIDSILA
                     YLYYTRGLEMSSILMLIYTFMALKGFLEWKKNYHERLVQKV"
     gene            complement(16753..18327)
                     /gene="ftsZ"
                     /locus_tag="PLS16_02050"
     CDS             complement(16753..18327)
                     /gene="ftsZ"
                     /locus_tag="PLS16_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNJ59888.1"
                     /translation="MEFEIFNENNSIIKVLGVGGGGCNAVNHMFAGGIPGVNFAVCNT
                     DNQDLQKSPVATKIQLGKDLLEGLGAGADPEEGRRAALEAEEEIRMYLSNNTKMVFVT
                     AGMGGGTGTGAAPVIAKIAKDLGILTVGIVTSPFMNEGKPKREKANRGIQNMKENVDA
                     LILISNNKLYEIFPDLDIDEAFGKADDILTTAAKGVTEIITKPGKINVDFNDVKRVMQ
                     NSGVAIIGIGHGSGENRAKDAIESAVNSPLLEEQDIYGAKGVVLYISYGSDFKMKELD
                     MIIQKVQNETETDCEVIWGVCKDESLGEDIAITLIATGFESGESNKERKKQLEQEQQR
                     KVQEEKNRVVHTLSNTSTNKLPEVNREEPSTNEQNTVELETKKNNIQIEFEIENLSTQ
                     NEAKIEEEDFEIKLVTKPRISIDDDFVVVNKSNQADLFKEDTFSNANRNLFSMEDQSL
                     ERDLIKKRIALEMQKNEVSLHEMEKQPAYKRYGIHLDQPTSAEKSEISNYVIYQNEVS
                     KSIEIKPNAMKDITLD"
     gene            complement(18375..19820)
                     /gene="ftsA"
                     /locus_tag="PLS16_02055"
     CDS             complement(18375..19820)
                     /gene="ftsA"
                     /locus_tag="PLS16_02055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNJ59889.1"
                     /translation="MQNILSLEMNEVKIDSEKIVCAIDIGTSKVAALVGKRNQFGKVE
                     ILGVGLSQSFGVQRGAVVNITRTVESIIKAVRQAEASSGVKFEKVHVGIAGQHIKSIK
                     NRSQVNRANTDTWITHEDVRLLKEESNKLALPPGDRIIHVLQQEFIIDNEPNIQDPVG
                     MMGVKLEGNFHIITGGISHIKNIERCVNAAGLEVASIQLEPLASADAVLSKQELEAGV
                     ALVDIGGGTTDIAIFENYIIRHTAVIPLGGNIITEDLRDFKILREQAERLKVELGSAI
                     ATDDLKDQQVRIKGIQPGAPGIELNLQSVAKVIGARMIEILANVDHQIRLSGYDKKLI
                     GGIVLTGGGSQLKHLIQLTELETGQVARLGYPREHLAPSKIKNIDNPIFATGIGLLIR
                     AFKMYDEEQLIAKEKNLISTIVEEPIAPVNVVEPIVIEPVQPKAEEVPVTTIDDEKTD
                     STKPLKKPSIFSVWGRKIENYMKSDNDFLDV"
     gene            complement(19832..20596)
                     /locus_tag="PLS16_02060"
     CDS             complement(19832..20596)
                     /locus_tag="PLS16_02060"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HNJ59890.1"
                     /translation="MNKQTKNIIIRLVVLLLVIGFFTVSLIARASVNNFKVADISILI
                     DELEDNFMISKDDVYYLITKNFSLKNQAIDNKTLFDIEQMVDTISCVESTQAFIDKNK
                     KLCVSIKQRTPIARVYNQLGQNFYIDKFGIKFMPKQNGTIKVPIITGVVTETLGGNDT
                     IKQKSLIETLKVCQYLIQENTWYSLIGQININEKKEIELIPRIGNALILLGNSNDLEE
                     KFNKLNVFYSEVLDKVGWEKYRVINIMYKDQAICLK"
     gene            complement(20589..21968)
                     /gene="murC"
                     /locus_tag="PLS16_02065"
     CDS             complement(20589..21968)
                     /gene="murC"
                     /locus_tag="PLS16_02065"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNJ59891.1"
                     /translation="MELDKIKNVYFLGIGGIGMSALARYFNILGKKVSGYDKTPTVLT
                     HELEQEGIDITFEDSVETLDKQADLVVLTPAIPKDSKQLAYYNSSDILVWKRAKVLGL
                     IANSGFNISIAGSHGKTSTSTFTAHLLKVGNKDIAAFLGGISINYQSNFIEGRTYIVA
                     EADEFDKSFLNLTPNIALITSVDTDHLDIYGNLDAIKASFSAFCKQIRPNGICIYNKQ
                     VDTKILNPEVKNLSYSLLDTTADFYTEHLSVNNGTYVFDVIHPNGKIENVICSYGGRH
                     NIENALGAIAIALQVGVDIAKIRTAIASFTGVKRRFETHFKNKRFVYIDDYAHHPREI
                     DAALAATRELYPNRKITAVFQPHLFSRTRDLCDEFAQSLSNVDELILLDIYPARELPI
                     EGITSQIILDKVTIPNKKIISRENLLSYLGNEIENIDVVITIGAGNIDTTVHSITELL
                     ENNSKEINE"
     gene            complement(21978..23087)
                     /gene="murG"
                     /locus_tag="PLS16_02070"
     CDS             complement(21978..23087)
                     /gene="murG"
                     /locus_tag="PLS16_02070"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNJ59892.1"
                     /translation="MGESKKYIIAGGGTGGHIFPAIAIADAIRQNNVDAEVLFVGASG
                     RMEMEKVPQAGYKIIGLEVVGIQRRLTIENLLVPIKLLKSLLHARKIIKEYHPNACIG
                     VGGYASAPVLFMAALMGIQTFIQEQNSYPGITNKILSKFANKIFVAYDNLNLFFNPNK
                     IKLTGNPVRKDISGTLPSKKDALDFFQLDEFKKTILVIGGSLGARTVNEAIEANLELF
                     VKQDYQVLWQTGKAYYTGIIERTEKLAIKQIKIHQFLKEMNLAYAAADVIVSRAGALS
                     ISELCIVGKPCVLIPSPNVSEDHQTKNAMALVHKNAALMIKDNEAIKKLGNVLVDLLN
                     NEQQQDALAVNIKMLAKSDAANEIVKEIISSNQNK"
     gene            complement(23101..24315)
                     /locus_tag="PLS16_02075"
     CDS             complement(23101..24315)
                     /locus_tag="PLS16_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNJ59893.1"
                     /translation="MFKNLINNIKGDKYIWYIAAILAVFSMLLVYSSARTLAYKSMHG
                     STEILLVKHLMNLGIGFGVIYLVHRVDHRYFSRIAQLMLILSIFLLAYTLVFGISTND
                     ATRWVKIPVIGLRFQSSDIARLALIMYTSRTISKMQGVIKDFKEVSIYLMGPIVLICA
                     LIMLENLSTAAILFMTCIVILFIGRVSTKYILFIFGGGLLVGGMLFALSFFKIVDFGR
                     ADTWVGRVDSWLHPDYSSDDLFQQKQANIAMAKGGFFRIAPGKSTQCNFLPEAYSDYI
                     FSTLVEEYGLVGAIFILCLYLFFLYRIVVLYNKSTKAFGALMAVGLGISITMQAVMHM
                     AVNTHLLPNTGITLPLISWGGTSIVANSIALGIILSVSAYVEKAPATNSEVGLVKKRS
                     PKKKDIVNDIHG"
     gene            complement(24340..25683)
                     /gene="murD"
                     /locus_tag="PLS16_02080"
     CDS             complement(24340..25683)
                     /gene="murD"
                     /locus_tag="PLS16_02080"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNJ59894.1"
                     /translation="MNNNRNIVLLGSGESGVGAAILAQKQGYGVFVSDFGTIKEVYKL
                     ELNKRNIEFEEGQHSEEKILAADIIIKSPGIPDKAPIIKKIKEKQIEIISEIEFAYRF
                     TKAKIIAITGSNGKTTTTSLVYHILKNAGLNVGLGGNIGDSFAKQVAEEDKEYFVLEI
                     SSFQLDNCTTFKPHIAILTNITEDHLDRYDYKFENYISSKFSIAKNQTEDDFFIYCLD
                     DEVSKKYLNQFDIKAKLIPFSYYQKLEEGGYIEDKNLIININKNKFTMPTNELALQGI
                     HNTYNSLAAGIGAKLFNVRNEKIRDSFQDFKSLEHRLEFVATVKGVDFINDSKATNVN
                     STWYALESMIKPTVLILGGVDKGNDYTLIKDLVKEKVKAIICLGKDNEALHQAFANEV
                     GYIVDTQSMEQAVNMAYNIAEKEEVVLLSPCCASFDLFENYEDRGRQFKRNVLNL"
     gene            complement(25690..26988)
                     /gene="mraY"
                     /locus_tag="PLS16_02085"
     CDS             complement(25690..26988)
                     /gene="mraY"
                     /locus_tag="PLS16_02085"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNJ59895.1"
                     /translation="MLYYLFKYLKEHYDFVGAGLFQYISFRASMAILLSLIITMIIGR
                     GIIKWLRKQQIGESVRDLGLEGQTQKKGTPTMGGIIIIAAILIPTLLFAKLDNIYVLL
                     LILTTVWMGGVGFIDDYIKVFKKDKEGLKGRFKIIAQVLLGLIIGAVLYFHPSVKVRD
                     FNNVFVYKYENGQVNWREPIEVKPTTKIIYESQGVQDGEYLYSHETKTSKTNVPFFKD
                     NLLDYGYVLKPFVNDYHKWAWIIFIPMVIFVLTAVSNAANLTDGIDGLASGVSAIVLI
                     TLAIFAYVSGNIKIADYLNVLYIPNSGEVVVVCAALIGACIGFLWYNAYPATVFMGDT
                     GSLMLGGVIATIAIILKKELLIPVLCGIFLVENLSVMIQVSYFKYTKKKFGEGRRIFR
                     MAPLHHHYQKMGYHETKIVTRFWIVSILLSVLTFLTLKVI"
     gene            complement(26999..28456)
                     /locus_tag="PLS16_02090"
     CDS             complement(26999..28456)
                     /locus_tag="PLS16_02090"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNJ59896.1"
                     /translation="MSILNEILYGLNYKMVGRDTIEIHELKIDSKSIQPNDVFIAIKG
                     TNVDGHSFIDTVIQAGARVIVCEQLPENINDTITYIQVEDTRKTAALLACNFYHHPSK
                     KTKLVGVTGTNGKTTIATLLYQLYRKAGFNVGLISTIENKINEEIIPATHTTPNPIAL
                     NQLLDLMIQQKCDYVFMEVSSHAAHQDRIAGLNFAGAIFSNISHDHLDYHKTFDEYIK
                     AKKIFFDQLPSSAFALVNIDDKRGEVMLQNTLAKKYTYALKSLADFNFKILENNISGL
                     VLKYKHQDIYTHLVGNFNAYNLLAILATAILLGMDEQEALGRISELQSAEGRFDIVKS
                     NRNNIVGIVDYAHTPDALKNVIDTVNAVRTGNESLITIIGCGGNRDKKKRPEMALIAA
                     TLSNKVILTSDNPRDEEPEQIIAEMQAGIPPHLAKKVVTILDRKEAIKTSVLLAQPND
                     VILLAGKGHEKYQEIKGVKYPFDDKEILNEVLNNI"
     gene            complement(28462..30564)
                     /locus_tag="PLS16_02095"
     CDS             complement(28462..30564)
                     /locus_tag="PLS16_02095"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HNJ59897.1"
                     /translation="MNIKTNILLRIYIVGILCAVFGFVVMGRVYQIQTERDGYWRNIA
                     DSLTTKVFTIPADRGNIYSAENELLATTVPYFNLSIDFGSTAMSDEIFKANIDSLAYY
                     MSVTKKDKSIQQYKKDLTTARKLKRRYYPIFKNIEFVQLNKIKQWPLFREGKYSGGLV
                     VETLQKRHNPFGYIAGRTIGYIKKDGPSVGLEGTYNSYLAGVSGIMPKQRIAGGTWMP
                     IHRAGSIEPKNGKDIHTTIDISVQDIAETTLMDAVINYNADFGCAVVMEVKTGAIKAI
                     ANLGKNKNGTYSETYNYAVGMGNEPGSVFKLAGYLALFDDGYITLNDSINTNHASATF
                     GKFRLTDDGHNTQYQFLKPDKAMAISSNVAIAKWVTQFYEGNKKKYYAKLAQFGLTTK
                     TKIELSGEPTPFIKSPDKWSIYSLPWMAHGYEVKLTPLQILAFYNAVANGGIRTQPHL
                     VNYISEGTNVIKTFDYTDGQRICSKEAALYATEILKSVVNDQSGTGRNIRTPYYQIAG
                     KTGTAKISFGKEGYSNKNLSTFVGFFPADNPLYSCIVVMGNPQGLVTTGGYVAAPVFR
                     TIADKIISSNVKNNLAINKDTSLKSINPPTFIANTQQIKSIAKLYDVKVDANHLDAEY
                     AYVDIDPVDKKVKVRTIKNDPTIIPNVRGMLLDDAIYLLESMGFKVGFTGKGKVQTQS
                     IESGQNFMKGSYIHLTLN"
     gene            complement(30576..30959)
                     /locus_tag="PLS16_02100"
     CDS             complement(30576..30959)
                     /locus_tag="PLS16_02100"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HNJ59898.1"
                     /translation="MEDEKDIATNSNILPVEKSKVQRIVEGILKLLGLDEFVSYIQVL
                     KNAFVIIVIVMVAIVEIFNTHLAERMTRNINKKKQHIKELRWEYMSANAEKNEKTKQS
                     EIQKIIAPESVKALQEPPKKVELEN"
     gene            complement(30959..31855)
                     /gene="rsmH"
                     /locus_tag="PLS16_02105"
     CDS             complement(30959..31855)
                     /gene="rsmH"
                     /locus_tag="PLS16_02105"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNJ59899.1"
                     /translation="MNYHQPVMLQECLEGLNLQPDGIYVDVTFGGGGHSKEILKRLGE
                     NGKLFAFDLDDDAQYNKIEDKRFVLIQANFRHLKRFLRLHNITKVDGILADFGVSSHQ
                     IDAADRGFSIRYNADLDMRMQQTQMLTAKQVVNTYSAEQLQNILGFYGEVPNAKTLAN
                     AIVQEREVKDIETTMQLVDLCLNYSRGKEHKYMAQVFQAIRIEVNDELNAIKDFLASA
                     LEILNVGGRLVTMSYHSLEDRLVKNFIKTGNVEGDVQKDFYGKIAKYFKLINKKPIVA
                     TEEEIKDNSRARSAKLRIAEKI"
     gene            complement(31866..32321)
                     /locus_tag="PLS16_02110"
     CDS             complement(31866..32321)
                     /locus_tag="PLS16_02110"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59900.1"
                     /translation="MLIFTGRYEIKIDAKGRLKLPADLLRQVPEETRTTYVLNKGLDN
                     CLRLYPIQQWKQITEEMAKQLSYFRNEDRQFLKYFYQGAINVELDGSDRILVPKRLQD
                     LVGIKSDIVINAYHDAIEIWGSEAYYADLKEPENFADMADAIWSKLSNK"
     gene            complement(32718..>33741)
                     /locus_tag="PLS16_02115"
     CDS             complement(32718..>33741)
                     /locus_tag="PLS16_02115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNJ59901.1"
                     /translation="FATNNTFTITDGRASTISNSKNGNVAYSDTLKGFDFIEFIRERL
                     VLLKMLLSDKGSIYLHIDYKIGHYVKIIMDEIFGIENFRNDITRVKCNPKNFARKGYG
                     NIKDMILFYSKSDNLIWNEPKTPYTDEDKIKLFQKTEKDGRRYTTIPLHAPGETQNGK
                     TSQAFKGILPPKGRHWRSDVAILEQWDNDGLIEWSNNGNPRKKIYFDEQEGKRMQDIW
                     EFKDPQYPVYPTEKNADLLDIIVKTSSNEDSIILDCFAGSGTTLKSAQTNGRKWIGID
                     QSEEAIKVIIAKLDRVEQDLFIDKSEYKHMTENQLNSQQGIDVNEEKVLRLKGNIISE
                     SLKLYQ"
BASE COUNT        11257 a         5812 c         4675 g        11997 t
ORIGIN      
        1 tttcttaaat gttcaggaag ttcattaaca atttcagcag gaatgtcatt tatcgtcggt
       61 tttctcgaaa gccattcttt tattttagcc ttttcttcca acaaaacttt attaacctta
      121 tcaatatatt ctgtctcggt aattacacct tttgctttag cagtcttaag caagtctgtt
      181 attttgttta agttttcact aagtgtttta ttgatttcta atgattctac tttctctgaa
      241 tattccgatt cggtaatcac gtttaatgat tttaactttg ctaaaatatc taattcgcta
      301 tctgcattta aactttcagt cttaatatct gtttgaggta attgcgcatt aatgtctgtc
      361 gtactattat ttagagtact ttcaaaaatt acacaaaaac ctacccaata actacaaaat
      421 cctaaagtaa tagtcgtaat gataattact ttaagcattt taaaaaaaga taatttagga
      481 aaaccactcc actcaaagtc tcttactttc caaccttttg cattaaattc attcattact
      541 gattgcactt cttctttttg gtctaaagtc aaccccgaga aaaaacacca ttttcggtat
      601 tcaaaatata aattctctgt catattgatt tgatatttgc tttcagtcat atttctatat
      661 tttggtcatc ttatcaatgt tcggagtgaa gtcctaccat tggttatata attaaggaat
      721 aacttcaacg ccatctacaa agaaatttgt gttgccttgc caaaagaaat ttttggcttt
      781 ttccttatat tgtaatctaa cttttttacc ttgcaatgta tctaattttg aagctacatt
      841 agcatctcta actgagaact tccacatatt attggcaatt gtattccctg gaagtgcatt
      901 aattccgcct aaatttaatt cgccttcata agtcttaaaa agatatcctt cttgattaaa
      961 agtaattaag acacctgccc tacttcctga actgcgcgta aagtttgccc aagcaaagaa
     1021 tcctacaatg ccaataatta agaaagcaaa taagattaaa aataattttt taaagaaact
     1081 aacagttgcc ttttttcctt tatggaaaat acttggttta ggttgaagat tggggtcttg
     1141 gctttcaatt actgtactca taacataaat attcttatca aaaatagtaa aaaaatgaat
     1201 gataacattt gctttcaaaa aataaattta aaatacttac ctttgataaa ataaattttt
     1261 tgaattatgg gatgttcagg ttgtggttct tctactccga atgggtgtaa gaacaacgga
     1321 cattgtgcga gtggtggttg ttcaaaactt gaggtgtatg attggttggc agatatttct
     1381 atagataatt tttcaacata caatatattt gaaatcagtt ttaaaaacgg ctcgcgcaag
     1441 ggtttttata aattagagaa accattagag gtttttgtag gcgattatgt agttgttgaa
     1501 gctactcaag gctatgatat tggacgtatc aatctaaaag gagaattggt taagctacaa
     1561 atgcgtaaga aaaaaatcaa tgaaaaaact tatgagttca agcaagtact tagaattgca
     1621 aaagaccacg agttggaatt gatggacaaa gtacgtgatt tggaaaaaga aacgatgtta
     1681 aaagctagag tattggcaag aaacttaggc ttagacatga aacttggagc tgttgaatat
     1741 caagcagatg gcaaaaaagc aactttttat tattttgccg atggcagagt cgattttaga
     1801 gaattgataa aagttctagc atctgaattt aagattaaaa ttgaaatgcg tcaagtgggt
     1861 gcaagacaag aagctgcaat cgttggtggc attggctctt gtggtcgtga gctatgttgt
     1921 tctacatgga tgactagttt taaatctgtg aatacaactg cggcaaggta tcagaattta
     1981 tctatcaacc aaaccaaatt gtctgggcaa tgtggaagat tgaaatgttg tttaaacttt
     2041 gaattggata cctatttaga tgcattgaga gatatgcctc aaaaggctga atttattgaa
     2101 agtgaagttg gtgttgcaaa gttaatcaag accgatattc taaaaggatt aatgacttat
     2161 attgagaaag aaaccaataa agcatacact ttaacttttg agaatgcgaa atctgttttg
     2221 gaaatgaata aagcaggaaa gaaacctgtg aacttaactg atttggccga agctcctgaa
     2281 gagcaaaatg acaatgctga atttgtagaa ttagttggtc agatttcttt atcttcatta
     2341 gaacgaagtg ataaaaaaag aaaacaaaaa gctagacagg ataataataa gcaacgcaat
     2401 aagcccaacc caaatgcttc aaataagcaa gaaggacaaa ctattgcaaa gaatgaggat
     2461 aagaaaaata cgcctacgga aaattcggaa ccaaaaccac aaaagtctaa taatagaccc
     2521 aatcatcata gacgaaacag aaagccagga gataataaac aatagttttt tagtaagcaa
     2581 acttatttcc tcgcctttgt tggtttgcaa ccaacaaact acttattttt cattgtgcaa
     2641 ttactcatct tcacttgctt tcaaatctta atttttttta tttaatatat aacattttta
     2701 ttgtgttgtt ggttacaaac caacaatggc aaagggagat gttgattttt tgtcgataat
     2761 taatattggt ttgtaaaggt ggactggctg gaaatgtgta tttttgcttt atgtctgaaa
     2821 agataaaatt actaccagat gcaattgcta atcaaatagc tgcgggtgaa gttattcaac
     2881 gtcctgcatc tatcgtaaaa gaattattag aaaattctgt tgatgctgga gcaaagaaca
     2941 tcaagctaat tgttaaagat gctggtcgtg cattggtaca agtcattgat gatggaagtg
     3001 gcatgtcaga aattgatgct cgtatgtgtt ttgaacgtca tgcgacttct aaaataagta
     3061 atatcaacga tttatttagc ttacatacaa tgggctttcg aggcgaagcc atggcatcta
     3121 ttgccgctgt ggctcatgtg gaattaaaga ctaaaatgca ggataaaaat gtcggtactt
     3181 gcgttattat agaagggtct aaaattattt tacaagaacc ttgtgctaca aatgatggca
     3241 cttcaatttc agtaaaaaat ttattttaca atgttccagc acgccgaaat tttctaaaat
     3301 ccaatcaaat tgaaaccaaa catattattg atgagttttt gcatgtagct ttagcaaatc
     3361 cttccatatt tttctcattg cataataatg gttctgaaat ttatcattta aaacaaggca
     3421 atttaagaca acgtattgtg agtgtttttg gcaaaaatca caatgacaaa ttagttccaa
     3481 ttgaagaaaa tacggaatat gtaagtgttt atggttttat tgggaaacct gaactagcaa
     3541 aaaaaacaag aggcgaacaa ttcttctttg taaacaatcg ttttattaaa agtaacttgt
     3601 tcaaccatgc gattcagagt gcttatgaaa acctaattca agataaaaca tatccatttt
     3661 attgtttatt tattgatatt ttaccttctg caatagatat taatgttcat ccaaataagc
     3721 aagaaataaa atttgaagat gagaaatctg tttatgcctt attgcgtgct tcagcaaaat
     3781 atgctttagc acaatatagc atcatgccaa ccttagattt tgaacatcaa gcaaatatca
     3841 attttctaac taatcctcaa agcagtgccg aaaatgagtt caagacctta ggttcaagaa
     3901 tgaataaaac tgaaatgaat cagcagcttc attctgaaca ttacgaggta agacatgcac
     3961 ctgcttttga taattgggaa aaactatatc aattagatgc tgaaactgaa caaaaaatta
     4021 taacacttca gagtaaattg gattacgaag atgatgaagt tttagagact aaagtgacat
     4081 ttaaaccaat ccaaattcat cagaaataca ttctgatgca ggtaaaaaaa gggttcatat
     4141 tgatagacca aaatcgagcc catcaacgta ttttatttga acaatattta gaaagattag
     4201 aaacaaaaga aagtagttcg caacaaactt tatttcctcg tactatcaat ttaagtgctg
     4261 gagattacca atatatggaa gaaatattac ctgatttacg aaaaattgga ttggatattg
     4321 aaccatttgg aaataatagc attattgttc atggtttacc acccgaattg gttaatgaag
     4381 atgaacattc gttaattgaa aatatcttac aacattataa agaaagtttg caaacggata
     4441 atttatctca acaccaacaa attgcaaaat cagtagccta tcataccgcc ataaaaccat
     4501 ctacaatact tgatgaaaaa agtatgcaaa atattataga tagcttattt gcttgtaagc
     4561 aacctaacat caatccagtt gggttaaaaa catttacgac atacacgata gaagatattc
     4621 aattattatt tgataaataa aaaatattaa caatccaaca acaagatttc gtttaatttt
     4681 gttgcgataa aatagatgag tttatgacac aatttggacg ctctggattt ctacaaaacc
     4741 ttacccctat cgtaaaaaat attatcctaa tcaatatcat tgtttttgtt atccaattta
     4801 tatttcctca ggtaacagat ttgttggcaa tgcattatcc gagcaatcct gcatttaaac
     4861 cttatcaatt atttacgcat ttatttgcac atggcggatt tatgcatatc ctttttaata
     4921 tgtatgcgtt gtttcttttt ggttccgtgt tagaaagaat tatgtcgcca aagagattca
     4981 tccaacttta ttttatttct ggatttggag caatagcctt tcatttatta atcagtgcaa
     5041 taatggccta taaaactgct ggaacggtaa tgattactta tgaaatggca gaagtcaata
     5101 aaacactttc agaaatttat tttcctggtt tagtgggtgc ttctggtgcg ttgtatggat
     5161 tgatggcagc ttttggtatt ttatttccaa atacgccttt gatgtttatg tttgtgccag
     5221 ttccaattaa agcaaaatat atgattccgc tggttatttt aatttatgat attggattcg
     5281 ctcaatatgg attagataat gttgcacatt atgcacatat tggaggagca atttttggtg
     5341 ctattgtcac tatttattgg agaaaaacag accgtagtag tttttattag ttggtttctt
     5401 cattgttttt ttttagaaga ttttgctcat aaggttttac aaatgtttct aacacatcta
     5461 agggaataat ttccttatga acttgagata aatctgcatc ttgctgagat acatcaagtg
     5521 aataaaccgc taaataataa ttattataac tagaaactgg aattttattc tttttcaact
     5581 gataggacaa ccaatacctc ataaatattg cagaatacca tctgtcttca tacttaaatg
     5641 accttccatt aaataataaa aacgcttcca ttcccttggg tcgaaatgaa attttatgtt
     5701 cagtattaga caataaacct ttagtaaaat agtcgtacaa gaagccatct tctcctttgt
     5761 attctatact caaccaaccg ccttcactag ctgggctagg tgcatacatc ttccaatatt
     5821 gaaattgaaa tgaattttga acaacagtag gaatattaat tgcattgaaa aatttgtaag
     5881 caatactttc gtgtttggtt ttatatacat ctaatcgagt ataaaacaat aagttttttt
     5941 gaacgatggt ataaagtaca aatattgtaa aaccataaat catcatcttt tgtgaattcg
     6001 atttaattgt actataaaac ttaaatcctt gttgtatttt aagtttctcc caaaatgatg
     6061 acggaataat tatcatggca gcagccacac cagttataga gaaattcgct acatctacta
     6121 aagaaaatat actaatatga aaacccaaga gataaataat tgcgaaatac cttaacacat
     6181 tatttttaaa ctttataaag agaaaaaatg gaaaaagata atctaataaa agtgtggcat
     6241 aggttaatgg catatataac caagcattat ctctaaagat ttcagcggtt gtgtatcgca
     6301 tccacagatc agaagttaga acttgaaccg cataaccatt tttccaagca tcgcctgttt
     6361 tggctaatcc tgtaataaaa taaaccattg caatttgaac aataacaata accgaaaccc
     6421 aaagcggtgt tttaatcttt tgtgtattat ttttaatact aaaaaaattt gacaaaggta
     6481 aaaaacatgc ccaagtcaat aattggaatg tgtatttatc aaaccctgta ttataacgag
     6541 tataagcttg taatatattg aaaaagaaaa aaagcaataa aaaagaaact attttagttt
     6601 gccaaccgat tgtaaaaagt acggctaaaa ctatagaaac actcatccaa aaataagcaa
     6661 agccatcatt tcgtacataa tcaaaaatag accagcttcc tattccattt gctttttgta
     6721 aaatgtttat tggaataata gtatgttctc cccaaaattc aaccgtatag ggtaatttta
     6781 atataataag agaatataaa atactaattc caagaacaat tctgaatata gccaccgaac
     6841 gctcatctaa tcgcaaatat tcaatagatt taatatattt attcaacttt gaattcatag
     6901 ctattcaaca aactacaaaa taatgataaa atagattaga aaagacaaat ttactttata
     6961 tttttatcgt atgatatttt ccaagagaaa cataatatac tatttattat ttacattcat
     7021 cacttttgtt ttttattctt ctgctataaa aaatggatat agcttagatg accaatatat
     7081 ttttaataac attccagctg aaggttcatc atttaaagat atatttagtg tatttgctaa
     7141 acgttttgac atttctgatt atcgtccaat tgcgatgttt acttttgctt tagaacaatt
     7201 tatacaaggc agtattaatc caaaggtatc gcatattatc aacctactta tttatgtaat
     7261 tttatgttgt actatatttt atgtattaca aagattgcct ataaaaaatg ctattcctat
     7321 tgcattagtt actactttac tttttatagt tcatcccgtt cacgctggtg ttgtcaataa
     7381 tctaaaaagt agagatggtt tgttcagtat gttgtttatt gtgttggcac ttaatagtta
     7441 tatcaattat gaagtaacga aaaagaggtt ttattttcta ttcattgctt tattttttgt
     7501 attgggtatt tattctaaga gagatgcgta taacttaata tttatcttgc ctttacttgc
     7561 attctttgtt tataatcgct ctttaaaaaa agatatttta ctctatttcg taggattatt
     7621 catgttaata attttatcta catcaataat agatttatta attccattta atgctaaagt
     7681 gtttgagaaa agttttatga taacagaaaa tccattaatt gctgacaata cattttttaa
     7741 tcgtttggga atgagtgtgg ctacttacct ctactattta aaatttatgg tcattccaaa
     7801 aggttattat ttctattttg gatttaatat gattccattg cgatcaattt taaatcctat
     7861 tacattagta caattagtgg tggttatctt gcctttattg gctgctttca ttatgtacaa
     7921 aaaaaataag cacttcacac taggtattct aattttctat agttcattat tgtattgtgc
     7981 caactttgtt actgaagtgc aaggcattgt tgcggataga tatgtattta tcgcaagttt
     8041 aggttttatt tttgcatttg cagttttaat tgttgatatt ttgccctcaa aaattgaaaa
     8101 gcaaattgct aatttgtcca aaacaataca agcaaaacat attcctattg taattacaat
     8161 tttaataatg ttgctatatt tcccatttgt aaaagcaaga aataatgctt ggtttagcaa
     8221 agaatcttta tttgaagcag atatgccaca tttacaacaa tcttttgaag ccaatagaat
     8281 tgcatctaca cattatgctc aagtgggtat gaatagtaga aacccaagcg aacaggaaag
     8341 taattttatg aaagctttac aatatgcaaa gcaagcaaat gcactaaatt ctgatgatat
     8401 ccatacacaa gaaaccgaag gtattgctta ttatggctta ggaaaaactg ctgaagctga
     8461 aaaacaattt ttaaaaatta taaagaaatt tgatacttca gttgtgagtt gggatttgat
     8521 gggagatatg acttttaaaa gacaaaatta tgatagtgct actctatgtt atgctaatgt
     8581 ctatcgatta gagaaaagca atgaatcggt atattataaa tatcctaatg ctttagtgca
     8641 aaatggcaaa agagatagtg catttaattt tttagaaaaa cttagtaaaa cttaccctga
     8701 ttggtatgtt ccttatgaaa gtgctgctta tttatattat caaaatggag atacttatta
     8761 tggtcattca tttttagttg ttgcattaga aaaaggatta aataataggc aaacttacga
     8821 gagaacaaag aatgaaataa ttcaaggtac tcagaatcca gatgtcaaaa taagtacggc
     8881 ttacaaacaa ctcttggttc gtatagaaaa aatccctata cctaactaaa tcatgtttac
     8941 actaactttc taagtagagt attcatattt accttatctg caataaattg ttctaaatta
     9001 tcaatactaa tacgctcttg ttgcatcgta tctctatctc gtaaagtaac cgtattatct
     9061 gtaagtgttt gatggtctat ggtaatacaa taaggcgttc ctattgcatc cattcttcta
     9121 tatcttttcc cgatagtatc tttttcttcg tagaaagttc ttccaacaaa tcttaatttc
     9181 gcttcaatct ctcttgcttt ttcaggcaaa ccatcttttt tagttaatgg gagaatagct
     9241 acttttattg gtgctaaagc tggtggtaaa ctcaatactg ttcttgtttc gccattttct
     9301 aatgtttctt cttttaagtt ttcactcata acacgcaaga acattcggtc taaaccaata
     9361 gaagtttcta ccacataagg tacataattt tcattgattt cactatcaaa atattgcatt
     9421 tttttacctg aatattgttg atggtttcct aagtcaaaat ctgttcttga atgaatacct
     9481 tccaactctt tgaatccaat aggaaaacga aattgtatat cttcagcagc atctgcatag
     9541 tgtgctaatt tttcgtgtac atgaaaacgt aaactttcag ggtttgttcc taaagccaaa
     9601 tgccaattta aacgtgtttc tttccacttt gcaaaccact ctttttgagt tccaggtttg
     9661 ataaaaaact gcatttccat ttgttcaaac tcacgcattc taaaaataaa ttgccttgca
     9721 acaatctcat ttctaaaagc cttaccaatt tgagcaattc caaaaggtat ttttaatcga
     9781 gcagacttct gcacatttaa gaaatttaca aaaattcctt gtgcggtttc tggtcttaaa
     9841 tatattgtgt tagattcttc agcaactgaa cctaattgtg ttccaaacat caagttaaat
     9901 tgacggacat ctgtccaatt tttagtgcct gatatctcac aaactatttc atattcttca
     9961 atcaaggctt tgtaacgtgc aaaatcacta gcttgtgcag cttcagtata tgctttttcc
    10021 aattctacac ctttggcata atctctactt gcttttgctt tatcctcttc agtagcattt
    10081 ttatctatgc cttttttttc taattctttc tgtccctttt ctttaaattt atcaatcttt
    10141 tcttccaaaa gcacatctgc tctatacctt ttttttgaat ctttattgtc aattaatggg
    10201 tctgaaaaag aatctacgtg acctgatgct ttccaagttg ttgggtgcat aaaaattgag
    10261 gcatcaatac caacaatatt ttcgtgtaat tgcaccatac ttttccacca atattcttta
    10321 atcgcatttt ttaattctac accaaatggg ccgtaatcat aaacagctcc caaattatca
    10381 tatatctcag atgaaggaaa aataaagcca tattctttgc aatgagctac tatcttctgt
    10441 aaactatctg tcgcttgtgc catattatat ttctacttaa ttttttaata ctattttata
    10501 ggaaaggaat ttttgtaact actgctttta ttaatttgtt tcttacttca ataaaaattt
    10561 cagtacctac ttttgagtgt gctaaatcta tgtaacccaa gccaattcca taacctaaag
    10621 ttggtgattg agttcctgat gttacatgac caatttcatt attatcggca tctttaatca
    10681 aataatgact acgaggaata cctctatcaa tcattttgaa accaactaat tttttactaa
    10741 ttccattttc tttttgtttc aataaaatat cttttgcaat aaaatcttta ttgaattttg
    10801 ttacccaacc caatccagct tctaatggtg aagtggtatc atcaatatca tttccataca
    10861 aacaaaaacc tttttccaaa cgtaatgtat ctctagctcc caaaccaatt ggttgtatgt
    10921 tttgtggctt acctgcttca aaaatttgat tccaaatagt tgctgcattt gatttgtcaa
    10981 aatagatttc aaaaccacca gcacctgtat atcctgttgc agaaataata atattatcga
    11041 tacctgcaaa tttcccaatt ttgaaatgat aatatggaat agaattcaaa tctacatctg
    11101 ttaagcattg taatgcctca gttgctttag gtccctggat tgccaataaa gcaatattat
    11161 ctgatatatt ttttaaatct gcattattgg tattgtgttg tgtaaaccaa gcccaatctt
    11221 tatcaatatt tccagcattc accacacaca tatattcttc gtcagccatt ttatacacca
    11281 ataaatcatc tacaattccg ccagtttcat taggcaagta accatacatc gcttttccaa
    11341 tttctaaagt tgaaatatca ttagaaataa ttttttgtaa aaatggtaat gcttcaactc
    11401 ctttcactat aaattcgccc atgtgtgata catcaaaaac gcctactccg tttctaacaa
    11461 tattatgttc ttcaatatct gaagtataac gaactggcat attaaatcct gcaaaatcta
    11521 ccatttttgc tccaagtgca atatgtgtat ccgtaagtgc tgtagttttc atctaaaatt
    11581 ataactgatt taaaaataat gccaaaaata ctgaattaaa ttctattaaa taaatttttc
    11641 aggataatgc caatttaaaa atataaaaac aaggttcgct tataaaaaaa tcgaataaac
    11701 ttcattttaa ttaagttatt ttcacattta cccaaattta ggtatgaaat taaccaattt
    11761 aggtattgca aaatgccttt tatcactata cttttgcgtc attattatta tttaatctta
    11821 gtctaaataa acaaaacaaa atgaatcgct taaaattatt attcgtagca ttatctctga
    11881 atggattggt gtatgcacag atggcagact taaaaatcaa aagtattgat ggggacaaaa
    11941 atggcattac cctatttcaa gcaagtttaa ttgaagctga caaagacact gttgctaacg
    12001 tagatggtgt tgctttgcta tcaaacattc cttatggttc ctatcagctg gaagttttga
    12061 gtcctggcta ccaaccttat acgacaactg tcaatttaaa cgaagaagaa caagacttga
    12121 ctgtaacact aacaactgat acaattttga acttagataa tgtagaaatt tttggggaac
    12181 gtaatgacaa acccaaaggt ttggaaatga ttacacgttt gcctatcaat ccaaatgaca
    12241 atatccaaac tgtttcggta atttcttata aagtaataga ggcacaagga gcattgacta
    12301 ttacagacgc tgttcgtaat attccgggtg taaccttatt cggaagttat ggtggcgtaa
    12361 aggaatcaat gtccacaaga ggttttagag gtgtgcctgt tttgaaaaat ggtatccgta
    12421 tggattcaca atttcaaaca gcatctggtg ttgtagatat gcaaggcgta gaatctattc
    12481 agatgataaa aggctctgct gcgattactc aaggtgtcat aacagatatt ggaaatgctg
    12541 gtggtgttat caatgtggtt acgaaaacac ccaactttac aaatttaggc aatgtgagtc
    12601 ttcgttatgg aagctggcat caagtccgtc cttcatttga tatccaaaga gttttaacac
    12661 aaaagaaaaa tattggtatt cgtttcaatg gtgtatatga atatggcaaa acttttagag
    12721 aaggaacaag tgcacatcgc ttctatttca atccatcttt tgcttggaaa gtatcagaaa
    12781 aaacaagttt tataatcgaa ggtgattatt ttaatgacaa tcgtactcca aaaaatgatg
    12841 cagtaaattt agcacctggt caaggagaaa atgcattata cattattccg aaagataaat
    12901 tcttaggatt taatactgat aataacaata ctaagatgtc aagctttatg gcaaggtttg
    12961 accacaacat taatgataaa ctaatgattc gtgcagctta tggtttctca acttatcaag
    13021 tagataatat ctcaacttca tcaaatgctg catttaaaaa caaagatttt aatttaagaa
    13081 aacgtgatgt tacaaaaagt ttgagagatg ataaaaactc aacattacaa tttgacttca
    13141 ttgctcagaa tataaaaacc ggaccattta aacatacgat tcaaactggt ttcgattata
    13201 gatctgccaa tgcaacgaca acttcttttg atgccgtaac tatcgatact attgatattt
    13261 atggagactt tacaaatgat ttaagtaaag tagttaagta cgataatatg ggcaatgaga
    13321 aattaggtgt caatgtagta ttcaatccta aaacgcctgt tgtatctaca tatcatactt
    13381 ttggtatctt agcacaagat attattgagt tacacaaata cctaaaacta tttattggtg
    13441 ttcgatattc tgaaatttat acaaaagacc cattaggaac gaaaggcaaa agacaatcag
    13501 cttggaatcc tagtgttggt ttaatgcttt ctccaacaga aaatataaat atttttggtt
    13561 cttataccaa ctctacaagt ttacgttctg caagtaattt aatgagtaac ggacaagaaa
    13621 ttggaccatc tagaactcaa caatttgaag ctggtataaa aactgcttgg ttcaaagata
    13681 gacttcgtta taacttaact tatttccata tttatacagc aaatttatct aattcagaat
    13741 atgtagaagg aacaaatgta actactggct attatttcaa agctggagat ttattaagag
    13801 atggaattga aacagagttg aatggacgta ttactaaaga tttaacagtt atggtaggtt
    13861 atgcattttt aaaagcacgt tataaaaatt caccttctta tgtaaatggt tctgctccaa
    13921 tgaatgctcc aacacacaca gcaaatggtt ggttacaata ccaattcagt caaggagcat
    13981 tgagaggatt gagtttaagt gctggtgttt attatgtagg aaaaagacca gtgaatgaat
    14041 acagcttaac gcctgatgga cacggaaata tgggtggtga aaagcctttt gatatgccag
    14101 catatacaac tttgaatgct caaattggct atacttacaa agcattttca gcaagattgt
    14161 atatgaataa ccttacgaat aaaataggat tgaattcata tttcagaggt ggttatatca
    14221 atcaaactga acctatcaat gcatcacttt ctattagtta taaattttaa aaaacatatc
    14281 aaatcccaaa caacaggcat cttcatcaca gaagatgtct gtttatttaa agcaaaaatt
    14341 attttatgaa aaaaaaggaa atatatactt ttaaaaaact tataggtgat ttacatttat
    14401 ggttaggtgt tgctagtggc attatattat ttgttatttg ttttactgga acaatttatg
    14461 tattcaaaac agaaattgaa gaatacttag agccacataa atataatata gaagctcaac
    14521 aacaaatttt acctattgaa atattaattg caaatactga aaaagaaact aaaggaaaag
    14581 tttcaagaat cgcttatacg gataagcaaa ctcgtcctta tgaaatcaat gtaaagacaa
    14641 atccggagga tagaagaggt actaatttta tggttaatca atatactgga aaagtaattg
    14701 gcagtgtaaa aggaccagca accgaattct tttttacgat gttcaaatta catagatggt
    14761 tattattaga tatggaaaaa ggcagaccta taacaggagc agcaacttta atctttacct
    14821 tcctatgtct atcaggattt atattatggt taccaaagaa aataaaagga ttgcgaagtt
    14881 tcaaaccagg attaaccatc atgtggcgtg ctagatggaa acgtatcaac catgacttgc
    14941 ataatgtatt aggattttat agtttaatat tcttattaat catgagtttg acaggcttaa
    15001 catggtcgta tgaatggtac aataacggac tttacaaatt actcacagga aaagaaatgg
    15061 aacagaaagg aggtccaggt ggtggcaaag gaaataaagg tgaaaaagga aaaaaggata
    15121 ataaatcaat tgaagctcaa gaatcaataa taattcctac aattacttat gaaaaagctc
    15181 ttgatattgc caatacaaaa ttagcttatc aaggtaaaac aataataaat aaaattgctc
    15241 aagatggaag tccttatgaa gttataaagt ttaataaaga ccgttttaac gaggaagcag
    15301 cagacaaatt aacgataaat gccacaagtg gaaatattga aaaagtagaa ttatttgatg
    15361 atttgtcatt aggcgaaaga gttatgaaac aaatacgtgg actacatgtt ggaactactt
    15421 atggaatgtg gtcaaaggta atttatttta tagtttgttt aatcgcaaca agcttaccaa
    15481 ttactgggat aatcatttgg ataaacaaaa tgaaatttgg ctcatcaaca gattaaaatt
    15541 actttttgat agatttatgt aaaaaatcac taacaatttt tatagctaat tgaattcgtt
    15601 catcataatt cccttcaata attttgaagg gaattttatt ttcacttaat gcattaaaat
    15661 ataattcaaa tagctcatct cttttttcag gatgttctct taattcatct tcttcccaat
    15721 caatatctgg cttacataat aaaaacaaat ctattgcttg atattggtat gcttgatgaa
    15781 taaaatcact tacttttccg aatttgaaag atgaccatat tttcattact aacatttctg
    15841 tatcataaaa acaaagttca tcatttgtat gcctttgcca agcttctaac tgaccttttg
    15901 caatcaaatc taaatcctca aacgtataat atccatttgt attttctaaa aaaatacgtg
    15961 cgaattcagg tacccaatgg caattaaagt tttgtgctaa ggtttttgaa agcgtagttt
    16021 ttcctgtaga ttctggacct gttatagcta ttcgtatcat actttttgta ctaatcgttc
    16081 atgataattt ttcttccatt ctaagaatcc tttcaaagcc ataaaagtat aaattagcat
    16141 caaaatactt gacatttcta aacctcttgt ataatacaaa tatgctaaaa tactatctat
    16201 tacaatccaa tatatccaat tttccaatac tttacgagca accatataag tcgtaaccaa
    16261 actaaaacat gtgcttagag catctaaaaa aggtgatgct tgaagggtat attttttaaa
    16321 aataaatccc aatattaaac tacaaatagt agtcaatatt attataaaaa agtgttgttt
    16381 taaattccat gtatgaattt gagttgtatc atttttattc caattcaaat aaccaacaat
    16441 tgccattata acataaaaaa cttgcaagat ggcatctaaa tataatttgg catcagcaca
    16501 aacataaaca taagttgcag cattaataaa tccaaaaatc caacagatta ctttaccttt
    16561 tatagcaaaa tagagatata aaataccaca aataatggct tgccactccc aataacttgt
    16621 tgcataaatt ccttgaaaaa aactactcaa aaaatcgttc atcgatgttc aagataaatg
    16681 aaaaaattaa gttttcaaat aaaaaagcga acctaaaagg ttcgcttaaa ttatagatta
    16741 ttacatttta atttagtcta atgtaatatc cttcatcgca ttaggcttaa tttcaatact
    16801 tttgcttact tcattttgat aaataacata gttagaaatt tcagattttt ctgcagatgt
    16861 tggttggtct aaatgtattc cgtatctttt atatgccggc tgtttttcca tttcatgcag
    16921 actaacttca tttttctgca tctctaaagc aatacgtttc tttattaaat ctctttctaa
    16981 actttgatct tccattgaaa aaaggtttct attagcattg ctaaaagtat cctctttaaa
    17041 taaatccgct tggtttgatt tgttcaccac aacaaaatca tcatcaatag aaatacgagg
    17101 ctttgttact aattttattt caaaatcttc ttcttcaatc tttgcctcat tttgagttga
    17161 tagattttct atttcaaatt caatttgtat attatttttt tttgtttcaa gttcaacagt
    17221 attttgctca ttagttgaag gctcttctct attcacttca ggcagtttat ttgttgatgt
    17281 attagataaa gtatgtacta ctctattctt ttcttcctga acttttcttt gttgttcttg
    17341 ctccaactgt ttttttcttt ctttattcga ttcgccagat tcaaatccag ttgcaattaa
    17401 agtaattgca atatcttcac ctaaactttc atctttacaa acaccccaaa taacttcaca
    17461 atcggtttct gtttcgttct gtactttctg gataatcata tccaactctt tcattttaaa
    17521 atcagaacca tagcttatat acaatacaac accttttgct ccatagatat cttgttcttc
    17581 caataatggc gaattaacgg cagattcaat cgcatctttg gctctatttt cgccacttcc
    17641 gtgtccaata ccaattattg caacaccact attttgcatg acccttttaa catcattaaa
    17701 atccacattg attttaccag gtttggtaat aatttcagta acaccttttg ctgctgtagt
    17761 taagatatca tcagctttac caaaagcttc atcaatatct aaatctggaa aaatttcgta
    17821 taatttattg ttagaaatta gaattaaggc atctacattt tctttcatat tttggatacc
    17881 tctattcgct ttttctcttt ttggtttacc ttcattcata aatggagaag taacaatacc
    17941 aactgtaaga atacctaaat ctttagcaat ttttgcaata acaggagctg caccagtacc
    18001 tgttccacca cccattcctg ctgtaacaaa aaccattttt gtattatttg ataaatacat
    18061 tctaatttct tcttcagctt ctaaagcagc gcgtctgcct tcttcaggat cagcaccagc
    18121 acctaaacct tctaataaat cttttcctaa ttgaattttg gtagcaactg ggcttttttg
    18181 taaatcttga ttgtctgtat tacaaacagc aaagttcaca cctggaatac cacctgcaaa
    18241 catgtgattc acagcattgc aaccaccacc gccaacacct aatactttaa tgatagagtt
    18301 gttttcgtta aatatttcaa attccataat tgtatatttt ttaattttta ttaatcttta
    18361 attattttta atttttaaac atctaaaaag tcattatcgc ttttcatata attttcaatt
    18421 ttccttcccc ataccgaaaa aatagatggt ttctttaatg gtttagtcga atccgttttt
    18481 tcatcatcta ttgttgttac aggaacttct tctgccttag gttgcacagg ttcaattaca
    18541 atcggctcta ccacatttac tggtgcaatc ggctcttcaa caatagtact tattaaattc
    18601 ttttctttag caatgagttg ttcttcatca tacattttaa atgcacgaat caataaacct
    18661 ataccggttg caaaaatagg attgtctata tttttaattt ttgatggtgc taaatgttct
    18721 cttggataac ctagtctcgc cacttgtcca gtttctaatt cagttaattg aattagatgt
    18781 tttaattgag agccacctcc tgttaaaacg attcctccaa ttaatttttt atcatatcct
    18841 gacaatctaa tttgatgatc tacattcgcc aaaatctcaa tcattctcgc accaattact
    18901 ttagcaacac tttgtaagtt taattcaatc ccaggagctc ctggctgaat tcctttaata
    18961 cgaacttgtt ggtcttttaa atcatctgtt gctatagcag aacctaattc aacttttaat
    19021 ctttcagctt gctcacggag aattttaaaa tctcttaaat cttcagtaat aatatttcca
    19081 cctaaaggaa taacagcagt atgacgaata atataatttt caaaaattgc aatatccgtt
    19141 gttcctccac caatatctac caaagcgaca cctgcttcta attcttgctt agacaaaaca
    19201 gcatctgcag aagctagtgg ttctaattga atacttgcaa cttcaaggcc tgctgcattt
    19261 acacaacgtt caatattttt tatatgagaa ataccaccag taataatatg gaaattacct
    19321 tctaatttca cacccatcat cccaactgga tcttggatat tcggttcatt atcgataata
    19381 aattcttgtt gtaaaacatg aataattcta tcacctggag gtaatgccaa tttattagat
    19441 tcctctttta acaaacgaac atcttcatgt gtaatccacg tatccgtatt cgctctattt
    19501 acctgacttc tattcttaat gcttttaata tgctgccctg caataccaac atgcactttt
    19561 tcaaacttta cgcctgaact tgcttctgct tgtctaactg ctttgataat agattcaaca
    19621 gttctggtaa tatttacaac agctccacgt tgtacaccaa aagattggct taatccaacg
    19681 cctagaattt ctactttacc aaactgattt cgttttccaa ctaatgctgc aacttttgat
    19741 gttccaatat caatagcaca tacaattttt tcggaatcaa tttttacttc gttcatttct
    19801 agtgataata tattctgcat atttctgaat tttattttaa acaaatagct tgatctttat
    19861 acattatgtt gataactcta tatttttccc accctacttt atccaaaact tctgaataga
    19921 aaacattgag tttattgaat ttttcttcta aatcatttga attgcctaat aaaataaggg
    19981 cattcccaat tcttggaatc aattcaattt cttttttctc attaatattt atttgaccaa
    20041 ttaaactata ccaagtattt tcttgtatta aatactgaca tacttttaat gtttcaatta
    20101 atgatttttg ttttattgta tcatttcctc caagcgtttc agtaacaaca ccagtaataa
    20161 taggcacttt gattgttcca ttttgctttg gcataaactt gataccaaat ttatctatat
    20221 aaaaattttg tcctaattga ttataaactc ttgcaattgg tgttctttgt tttatgctaa
    20281 cacataattt tttattctta tcaataaatg cttgagtcga ttctacacac gaaattgtat
    20341 ctaccatttg ttcaatatca aacaatgttt tattatctat tgcttggttt tttaaactga
    20401 aatttttagt gattaaataa taaacatcat cttttgaaat cataaaatta tcttccaact
    20461 catcaatcaa aatggatata tcagccactt taaaattatt gacacttgct cttgcaatca
    20521 atgaaacggt aaagaatcct attactagaa gcaatacaac caaacggata ataatatttt
    20581 tagtctgctt attcattaat ttctttacta ttattttcta ataattcagt aattgaatgc
    20641 actgttgtat caatatttcc agcaccaatt gtaattacta cgtcaatatt ttctatttca
    20701 tttcctagat aactcaataa gttttctctt gaaataattt tcttatttgg aatcgtaact
    20761 ttatctaata taatttggct cgtgatacct tcaattggca actctctggc aggataaata
    20821 tctaataaaa tcagttcatc tacattagaa agagattgtg caaattcatc acataaatct
    20881 ctagttcttg aaaatagatg tggttggaaa acagctgtta tttttctatt tggatataat
    20941 tctctcgttg ctgccaacgc ggcatctatt tctcttgggt gatgtgcata atcatcaata
    21001 tagacaaaac gtttattttt aaaatgtgtt tcaaatcttc ttttaacacc agtaaaacta
    21061 gcaatagctg ttctaatttt tgctatatca acacccactt gcaaagcaat agctatcgca
    21121 cctaatgcat tttcgatatt gtgcctgcct ccatagctgc aaataacatt ttcaattttt
    21181 ccgtttggat gaataacatc aaatacataa gttccattat ttacgctaag atgttcagta
    21241 tagaaatctg cagtcgtatc caacaaacta taactcaaat ttttgacttc aggatttaga
    21301 attttagtat ctacttgttt attgtaaata caaattccat ttggtctaat ttgcttacaa
    21361 aatgcactaa aagaagcttt aattgcatct aaatttccat aaatatccaa gtgatcggta
    21421 tccactgaag tgatgagtgc aatatttgga gtaaggttta aaaacgactt atcaaattca
    21481 tcagcttctg caacaatata ggttctgcct tcaataaaat tggattgata attaatcgaa
    21541 attccaccta aaaacgccgc aatatcttta ttgccaactt taagtaaatg tgctgtaaat
    21601 gtagaagtgg aagtctttcc atgcgaaccc gcaatcgaaa tattaaatcc tgaatttgca
    21661 attaatccta atacttttgc acgtttccaa actaaaatat cagatgaatt ataatatgct
    21721 aattgtttgc tatcttttgg aattgctggt gtaagaacaa ctaaatcggc ttgtttatct
    21781 aaagtttcaa cactatcctc aaaagtaata tcaattcctt cttgctctaa ttcatgcgtt
    21841 aatacagttg gggttttatc gtagccactt acttttttac caaggatatt aaaataccta
    21901 gctaaagcag acattccaat tccaccaatt cctaaaaagt aaacgttttt tattttatct
    21961 aactccatct aaataactca tttattttga ttactactaa taatttcttt aacgatttca
    22021 tttgcagcat ctgattttgc taacattttt atattcactg ctaaggcgtc ttgttgttgt
    22081 tcattattca ataaatcaac aagtacgttc cctaattttt ttatcgcttc attatctttt
    22141 atcatcaaag cagcattctt atggactaat gccattgcat ttttagtctg atggtcttca
    22201 gaaacatttg gtgaaggaat taggacacaa ggcttaccaa caatacaaag ttctgaaata
    22261 gacaaagctc ctgctctcga cacaatcaca tctgcagcag cataagctaa attcatttcc
    22321 ttgaggaatt gatggatttt aatttgttta atagccaact tttctgttcg ttcaataatg
    22381 cctgtataat aggcttttcc tgtctgccac aagacctgat aatcttgctt tacaaacaac
    22441 tctaaattag cttcaattgc ttcattaact gttctagctc caaggcttcc accaattact
    22501 aaaatcgtct ttttgaattc atctaattgg aaaaaatcta aagcatcttt ctttgatggc
    22561 aaagttcctg aaatatcttt ccttacagga tttcctgtaa gttttatttt atttggatta
    22621 aaaaataaat tcaaattgtc ataagcaaca aatattttat ttgcaaactt ggataaaatt
    22681 ttattggtaa taccaggata agaattttgt tcttgaataa atgtttgaat gcccatcaaa
    22741 gctgccataa acaataccgg tgcagatgca taaccaccta caccaataca tgcattgggg
    22801 tgatattctt taataatttt acgtgcatgc aataaacttt tcaataattt aattggcact
    22861 aataaatttt caattgtcaa tctacgttga attccgacaa cctctaatcc aataatttta
    22921 tagcctgctt gtggtacttt ttccatttcc attcttccgc ttgctccaac aaataatact
    22981 tctgcgtcaa cattattttg gcgtatcgca tcagcaatag caatagctgg gaagatatgt
    23041 ccaccagttc ctcctcctgc aataatatat tttttacttt ctcccaattc cttttattta
    23101 ttatccatga atatcattaa caatatcctt tttcttagga cttcgctttt ttactaaacc
    23161 aacttctgaa tttgttgctg gtgctttttc aacataagca cttacactca aaataatacc
    23221 taaggctatt gagttagcga ctatacttgt acctccccaa cttattaatg gcaaagttat
    23281 tccagtattc ggcaataaat gcgtatttac agccatgtgc atcacagcct gcatcgtaat
    23341 actaatacct aatccaacag ccattaaagc accaaatgct tttgtacttt tattatataa
    23401 tacaacaatc cgatacagaa aaaacaaata caaacacaaa ataaaaatcg ctccaactaa
    23461 accatattct tccactaaag ttgaaaatat ataatccgaa taagcctctg gcaaaaaatt
    23521 acattgtgtg ctttttcctg gagcaattct aaaaaagcca ccttttgcca tagcaatatt
    23581 agcttgtttt tgttgaaata ggtcatcgct actataatca ggatgcaacc aagaatcaac
    23641 acgacccacc caagtatccg ctctaccaaa atctacaatc ttaaagaaag aaagtgcgaa
    23701 taacataccg ccaacaagca ggcctccacc aaatataaac aagatgtatt tcgtactcac
    23761 tctgccaata aataagataa caatacaagt cataaataag atagcagcag tagataaatt
    23821 ttctaacata attaatgcac aaatcagcac aattggaccc atcaaataaa tacttacttc
    23881 tttaaaatct tttattacac cttgcatctt agatattgtt cttgaagtgt acattatcaa
    23941 agctagacgt gcaatatctg atgattggaa tctcaaacca attacaggaa tcttaaccca
    24001 tctagttgca tcgttagtac taattccaaa aaccagcgta taagccaata aaaatatcga
    24061 tagtatcaac attaactgtg ctatcctaga aaaatatcga tgatctactc tatgaactaa
    24121 atagataacg ccaaaaccaa ttcctaaatt cattaggtgt tttactaata atatttctgt
    24181 acttccatgc attgatttat atgccagcgt tcgagcagaa ctatacacca ataacataga
    24241 aaagacagcc aatatggcag ctatgtacca tatatactta tctcctttta tattattgat
    24301 taaattctta aacattactt agtttttcaa tttctagtat tatagattca aaacatttct
    24361 tttaaattgt ctgcctctat cttcataatt ttcaaataaa tcaaagcttg cacaacaagg
    24421 tgataataat acgacttctt ctttttcagc aatattataa gccatattta cagcttgttc
    24481 catactttgt gtatccacga tgtatccaac ttcattagca aatgcttgat gtaaagcctc
    24541 attatcttta cccaaacaaa ttatcgcttt aactttctct ttgactaaat ctttaatgag
    24601 cgtataatcg tttcctttat ccacaccacc aagaatcaat actgttggct taatcataga
    24661 ttctaaagca taccaagttg aattgacatt tgtagctttt gagtcattga taaaatcaac
    24721 acccttgaca gttgcaacaa attctaaacg atgttctaaa gatttaaaat cctgaaaact
    24781 atctctaatt ttttcatttc ttacgttaaa gagttttgct ccgattccgg cagccaaact
    24841 attatacgtg ttgtggattc cttgtaatgc aagttcgttt gttggcatgg taaatttgtt
    24901 tttgtttata ttaataatga gattcttatc ttcaatgtat ccgccttctt ctaatttttg
    24961 ataataagaa aatggaataa gttttgcttt tatatcgaat tgatttaagt attttttgct
    25021 tacttcatca tctaaacaat agatgaaaaa atcatcttct gtttgatttt tagcaataga
    25081 aaacttagag ctaatataat tttcaaactt atagtcgtat ctatccaaat ggtcttcagt
    25141 aatattggtt aagatggcaa tatgtggttt gaaagttgtg caattatcta attggaaaga
    25201 agaaatctct aatacaaaat attctttatc ttcttctgcc acttgcttag caaagctatc
    25261 tccaatattt ccgcctaaac caacgtttaa ccctgcattc ttcagaatat gatagactag
    25321 tgatgttgtt gtagttttcc cattagaacc tgtaattgca ataattttag cttttgtaaa
    25381 tctataagca aactcaattt cagatataat ttctatctgt ttttccttta tttttttgat
    25441 aattggagct ttatcaggaa tgccaggact tttaattata atatcagctg ctaatatttt
    25501 ttcttcagaa tgttgtcctt cctcgaactc aatatttcgc ttattcaatt ctaatttata
    25561 tacctcttta atagttccaa aatcagaaac aaatacacca tatccttgct tttgtgcaag
    25621 aattgccgct ccaactccac tttcgccact acctaataat actatgtttc tattgttgtt
    25681 cactatcgtt tatattactt ttaatgttaa aaatgtaagc actgaaagta gaatgcttac
    25741 tatccaaaat ctagttacaa tttttgtttc gtggtaaccc atcttttgat aatgatgatg
    25801 caaaggtgcc attctgaata tccttctacc ttcaccaaat ttcttcttcg tgtatttgaa
    25861 ataagaaact tgtatcatta ccgataaatt ttctactaag aaaattccac acaaaacagg
    25921 aatcaataat tctttcttta agattatagc tatcgtggca ataaccccac ccaacattaa
    25981 agagccagta tcgcccataa aaactgttgc aggataggca ttgtaccata aaaaaccaat
    26041 acaagcacca attaaggcag cacaaaccac cacgacttct ccagaattag gaatatataa
    26101 tacattcaaa taatctgcaa ttttaatatt cccagataca taagcaaaga ttgctaaggt
    26161 aattagtaca attgcggaaa cgccagatgc caaaccatca attccatcag tcagatttgc
    26221 ggcattagaa acggcagtaa gcacaaaaat aaccatagga ataaaaatta tccaagccca
    26281 tttgtggtaa tcatttacaa atggttttaa gacataacca taatccaata aattatcttt
    26341 aaaaaatggt acattggttt tgcttgtttt tgtctcgtga gaatataagt actctccatc
    26401 ttgaacacct tgactttcat atataatttt tgtagttggt ttgacctcaa ttggctctct
    26461 ccaatttact tgaccatttt catatttata tacgaagaca ttattaaaat ctctaacttt
    26521 tacagaaggg tggaaatata aaaccgcacc aataattaat cctaataaaa cttgagcaat
    26581 aattttaaat cttcctttta aaccttcttt atcttttttg aatactttta tataatcatc
    26641 tatgaagcct acacctccca tccaaacagt tgttaatatc aacaacaaca cataaatatt
    26701 gtctaatttt gcaaataata aagtaggtat taaaatagcc gcgataatga tgattccacc
    26761 cattgttggt gtgccttttt tttgtgtttg cccctctaat cccaaatcac gcacactttc
    26821 tccaatttgt tgtttacgca gccattttat tattcctcta ccaattatca tcgtaattat
    26881 caaagacaat aaaattgcca tagatgctcg aaatgagata tattggaata aacctgcacc
    26941 aacaaaatca taatgttctt ttaggtattt gaataaatag tatagcatct tctatttttt
    27001 atatattatt taatacttca tttaatattt ctttatcatc aaacgggtat tttacgcctt
    27061 ttatctcttg atatttctca tgtcctttac cagccaacaa aatgacatca ttcggttgag
    27121 ctaataaaac acttgtttta attgcctctt ttctatctaa aatggtaact acttttttag
    27181 ctaaatgagg cggaatacct gcttgcattt cagcaataat ttgttcaggt tcttcatctc
    27241 taggattatc agatgtcaaa ataactttat tactgagcgt tgctgcaatc aaagccattt
    27301 ctgggcgttt cttcttatct ctattacctc cacaaccaat tatagtaatc aatgattcat
    27361 tgcctgtcct tactgcattt acagtatcaa taacattttt taaagcatca ggcgtgtgtg
    27421 cataatctac aattccgaca atattatttc gattagattt aacaatatca aatctacctt
    27481 cagcactttg taattcagaa atacgaccaa gtgcttcttg ttcatccata cccaataaaa
    27541 tagccgttgc taaaatggct aatagattat atgcattaaa attgccaact aaatgcgtat
    27601 aaatatcttg atgtttatat ttcaagacta aacctgatat attattctct aaaattttaa
    27661 aattaaaatc agctaatgat tttaaagcat aagtgtattt tttagctaat gtattttgca
    27721 acattacttc accacgttta tcatcaatat tcaccaatgc aaaagctgat gatggcaatt
    27781 gatcaaagaa tattttcttt gccttaatat attcatcaaa tgttttgtga taatctaaat
    27841 ggtcgtgaga aatatttgaa aaaattgctc ctgcaaaatt taagcctgca attctatctt
    27901 gatgtgctgc atgtgaactt acttccatga atacataatc acacttttgt tgtatcatta
    27961 aatccaacaa ttggttcaat gcaattgggt ttggtgtcgt atgtgttgca ggaataattt
    28021 cttcattgat tttattttct attgtagaaa ttaaaccaac gttaaaacca gcttttctat
    28081 ataattgata taataaagta gcaattgtag tcttgccatt ggtgccagtt acacctacaa
    28141 gttttgtctt tttagatgga tgatgataaa aattacatgc taataaagca gctgtttttc
    28201 ttgtgtcctc tacttgaata taagttatgg tatcattgat attttctggt aattgttcac
    28261 aaacaataac tctggcacct gcttgaataa ctgtatcaat gaatgaatga ccatcaacat
    28321 ttgtaccttt aattgcaata aaaacatcat ttggttgaat acttttagaa tcaattttta
    28381 attcatgaat ttcaatagta tcacgcccta ccattttgta gttcaatcca tataatattt
    28441 catttaatat gctcaattct tttagtttag tgttaaatgg atataacttc ctttcataaa
    28501 attctgtcct gattctatag attgcgtctg cacttttcct ttgcctgtaa agccaacttt
    28561 aaagcccata ctttctaata aataaatagc atcatctaac aacattcctc tcacatttgg
    28621 tatgattgtc ggatcatttt ttatggttct aactttaact tttttatcaa ctgggtcaat
    28681 atctacataa gcatattctg catctaaatg attggcatct accttaacat catataattt
    28741 tgcaatagat tttatttgtt gtgtattcgc aataaatgta ggaggattga tgcttttcaa
    28801 tgatgtatct ttatttattg ccagattatt ttttacattg gaagaaataa ttttatcagc
    28861 aatagttcta aaaactggtg cagcaacata accgccagtt gttaccaaac cttgagggtt
    28921 tcccattacc acaatacatg aatacaatgg attatcagca gggaaaaagc ctacaaaggt
    28981 ggataaattt ttattagaat aaccttcttt cccaaaactt atttttgctg ttccagtttt
    29041 acctgctatt tgataataag gcgtacgaat atttcttcct gttccacttt gatcatttac
    29101 gactgattta agaatttctg ttgcatataa agcggcttct ttagaacaaa ttctttgtcc
    29161 atctgtataa tcaaaagtct taataacatt agttccttca gaaatataat tcactaaatg
    29221 tggttgtgtt ctaattccac catttgcaac tgcattatag aaagctaaaa tttgtaaagg
    29281 tgtaagtttt acttcataac cgtgtgccat ccaaggtaaa gaataaatcg accatttatc
    29341 aggcgatttt ataaatggag ttggctctcc agaaagctct attttagttt ttgttgttaa
    29401 tccaaactga gccaattttg cataatattt tttcttattt ccttcataaa attgcgttac
    29461 ccatttagct atagccacat ttgaggaaat agccatggct ttatctggtt ttagaaactg
    29521 atattgagta ttatgtccat catctgtcaa acggaatttc ccaaaagtag ctgatgcgtg
    29581 gttagtatta atagaatcat ttaaggtaat ataaccatca tcaaacaatg ctaaatatcc
    29641 agctaattta aaaacagaac ccggctcatt acccattccc acagcataat tataagtttc
    29701 actataagtt ccatttttat ttttaccaag attggcaatt gctttaattg caccagtttt
    29761 tacttccata acaacagcac agccaaaatc tgcattgtag ttaataacag catccattaa
    29821 tgtagtctca gcaatatctt gaaccgaaat atcaattgtg gtatgtatat cttttccatt
    29881 tttaggctca atacttcctg ctctatgaat aggcatccat gttccaccag caattcgttg
    29941 tttaggcatt atgccactaa cacctgctaa ataagaatta taagtacctt ccaaaccaac
    30001 acttggacca tcttttttaa tatatccaat cgttcttcct gcaatatatc caaaaggatt
    30061 atgtcttttt tgtagtgttt cgacaaccaa acctccagaa tattttcctt ctctaaaaag
    30121 cggccattgc ttaatcttat tgagttgtac aaattctata tttttaaaaa ttggataata
    30181 ccttcttttc aattttcttg ctgtcgttaa atccttttta tattgctgta tagacttatc
    30241 tttcttagtt acagacatat aataagctaa agaatcaata tttgctttga aaatttcatc
    30301 actcatggct gttgaaccaa agtcaataga taaattaaag taaggtactg ttgttgctaa
    30361 taattcattt tcagcagaat aaatatttcc tctatcagcg ggaattgtaa aaacttttgt
    30421 tgtcaaagag tctgcaatat ttcgccaata accatctctt tctgtttgaa tttgataaac
    30481 cctacccatt acaacaaaac caaatacagc acacagaatg cctactatat atattcttaa
    30541 taatatgttg gttttaatat tcaatgtatt cctttttaat tctctagttc aactttcttt
    30601 ggtggctctt gtaatgcctt tacactttct ggagctatta ttttttgtat ttctgattgt
    30661 ttagtttttt catttttttc agcatttgca gacatgtatt cccatctcag ttcttttata
    30721 tgttgttttt ttttgttgat atttctagtc attctttctg ccaaatgcgt attaaaaatt
    30781 tcaacaattg caaccatcac gataactata attacaaatg cattctttaa tacttggata
    30841 taacttacaa attcatctaa acccaataat ttgagtattc cttctacaat ccgttgtact
    30901 ttagatttct caacaggtag aatgtttgaa ttagttgcta tatctttttc atcttccatt
    30961 atattttctc tgctattcgc aattttgcac ttcttgctct tgaattgtcc tttatttctt
    31021 cttcagttgc aacaattggt tttttattaa ttagtttaaa atatttagct atctttccat
    31081 agaaatcctt ttgtacatct ccttctacat tgcctgtttt gataaaattc ttaaccaatc
    31141 tatcttctaa tgaatgatag gacatggtaa ccaatcgacc gccaacattt aatatttcta
    31201 atgctgaagc caaaaaatct ttaatcgcat tgagttcatc attcacttca attcgtatcg
    31261 cttgaaaaac ttgagccata tacttatgct ccttccctct tgaatagttc aaacacaaat
    31321 ccactaattg catagtagtt tctatatctt ttacttcacg ctcctgaact attgcatttg
    31381 ctaatgtctt agcgttcggc acctcaccat aaaagcctaa aatattttgc aattgctcag
    31441 cagaataggt attcaccact tgtttagcag taagcatttg agtttgttgc atacgcatat
    31501 ccaaatcggc attataacga atagaaaagc ctctgtcagc agcatcaatt tgatgagagg
    31561 acactccaaa atcagctaaa ataccatcta ccttcgtaat attatgaagt cgtaagaacc
    31621 tttttaaatg tctaaaatta gcttgtatca acacaaatcg tttatcctct attttattat
    31681 attgagcatc atcatctaaa tcaaaagcaa ataattttcc attttctccc aatcttttta
    31741 gaatctcttt tgaatgtcca ccaccgccaa aggttacatc aacatagata ccatcaggct
    31801 gtaaattcaa tccttctaaa cattcttgca acataaccgg ttggtgataa ttcattatat
    31861 tccgattatt tgttacttaa tttactccaa atcgcatcag ccatatctgc aaaattttca
    31921 ggttctttta aatctgcata gtatgcttca ctaccccaaa tttcaatagc atcatgatag
    31981 gcatttatca caatatctga tttaatacct actaaatctt gcaaacgttt aggtactaaa
    32041 attctatcac taccatctaa ttcaacattg atagctcctt gatagaaata ttttaaaaac
    32101 tgtctgtctt catttctgaa atagcttagt tgttttgcca tttcttcagt gatttgtttc
    32161 cattgttgga taggatacaa tcgcaaacaa ttatctaatc ccttatttaa aacatacgtt
    32221 gttcttgttt cctcaggcac ttgacgtaat aaatccgcag gcagttttag tctgcctttt
    32281 gcatctattt taatttcata tcgtcctgtg aaaatgagca ttgctgtata tcttatacaa
    32341 aattatataa aacctgccat tttctgccac tttctgcata aaattcctac tacaaacagt
    32401 gtggataatt gaaaatatgt ggataatctt gtatataagt aatactacga tgtgaaaata
    32461 aagctgagat gcctaatttt attatattgt ttatcaacaa acgttaatca acaaaaatta
    32521 attttatgga aaacttctaa aaaattggtg ggagtgagtg gcaggatttc tgtgttttta
    32581 ctcaaaaaag gaacaaattc tattcaaaaa gtcgttttac ttagctgaat ttgaactgaa
    32641 ataaatagaa aatgataaaa attaaatact agagtttgat tcgtaattta acgatagcag
    32701 acgacttttc aatactatca ttgatacaac ttcaaagatt cagaaattat attacctttt
    32761 aatcgaagaa ctttttcctc atttacatca attccttgtt gtgaattaag ttgattttca
    32821 gtcatgtgtt tgtattctga tttatcgata aataaatctt gctctactct atcaagtttt
    32881 gcaattatta ctttaatagc ttcttccgat tggtcaattc caatccattt ccgtccgtta
    32941 gtttgtgcag attttaaagt agttccagaa cctgcaaaac aatctaaaat aatgctatct
    33001 tcattcgatg aagtcttaac aataatatcc aataaatcag catttttctc ggttggataa
    33061 acaggatatt gcgggtcttt aaattcccaa atatcttgca tacgtttacc ttcctgttcg
    33121 tcaaaataga ttttctttct cggatttcca ttgttgctcc attcaattaa accgtcattg
    33181 tcccattgct ccaaaatagc tacatcactt ctccaatgtc tcccttttgg tggcaaaatt
    33241 cctttaaatg cttgtgaagt ttttccattt tgcgtttcgc ctggtgcgtg aagcggaatt
    33301 gttgtatatc ttcttccgtc tttttcggtt ttttgaaaaa gtttgatttt atcttcatcg
    33361 gtgtaaggtg ttttcggttc gttccaaatt aaattgtcag atttagaata aaacaaaatc
    33421 atgtctttga tatttccata accttttctt gcaaagtttt tcgggttgca ttttaccctt
    33481 gtaatgtcat ttcgaaaatt ctcaattcca aaaatttcgt ccattatgat ttttacataa
    33541 tgtccgattt tataatcaat gtgtaaataa attgaacctt tatcagagag caacattttg
    33601 agtaaaacca aacgttcacg aataaattct ataaaatcaa aaccttttaa agtatctgaa
    33661 taagcaacat ttccattttt agaattactg atggtactag ctcttccgtc tgtaatagta
    33721 aatgtattgt ttgttgcaaa c
//
LOCUS       DAOQCW010000012        33244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_479_length_33244_cov_6.726054, whole genome shotgun sequence.
ACCESSION   DAOQCW010000012 DAOQCW010000000
VERSION     DAOQCW010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 33244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33244
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_479_length_33244_cov_6.726054"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /gene="atpH"
                     /locus_tag="PLS16_02120"
     CDS             complement(<1..521)
                     /gene="atpH"
                     /locus_tag="PLS16_02120"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNJ59902.1"
                     /translation="MSAEKLANRYADAIFSESTSTNKLEQSYADLQVVAGAIAESREF
                     SNYLKSPIIDSSKKLVAFKEIFESKVQASTMQFLDIIINNKREQYLPLILSKFFSKYN
                     QIKGIAEVNVTVASELDADTQKKIEDYIKKESGKANVKINQKIDASILGGFIVDFGDK
                     ILDNSVKQKLKKV"
     gene            complement(521..1036)
                     /gene="atpF"
                     /locus_tag="PLS16_02125"
     CDS             complement(521..1036)
                     /gene="atpF"
                     /locus_tag="PLS16_02125"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNJ59903.1"
                     /translation="MLLKFDTLSLLTPDLGLVFWTTIIFLVFWFIVGKKALGPIANAI
                     KDREASINDALNAAEKAKAEMSQLQADNANLLKEAREESAKILKEAKDIKESVIADAH
                     NRAKDEAAKIIADAKAEIDTQKKKALEEVKNELSQFSVEIAEKILNRELSTSKDHQQY
                     INSLVENINNN"
     gene            complement(1103..1306)
                     /gene="atpE"
                     /locus_tag="PLS16_02130"
     CDS             complement(1103..1306)
                     /gene="atpE"
                     /locus_tag="PLS16_02130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNJ59904.1"
                     /translation="MTGFAALGAGLAALAAGIGIGLIGSNSVQSIARQPEAANDIRGA
                     MILTAALIEGVAIIGMILSFLMN"
     gene            complement(1351..2457)
                     /gene="atpB"
                     /locus_tag="PLS16_02135"
     CDS             complement(1351..2457)
                     /gene="atpB"
                     /locus_tag="PLS16_02135"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNJ59905.1"
                     /translation="MKYIFLALFSLTFAINPALANTDTVANEGIMLINEKSIAHEEVE
                     KEETDIVETILHHVSDANENHLGTMGHTHLTIPLPCILINKETKKVSFFMSSAFHHAP
                     EGIIDGYKMEHSRVVDAQTGKRDTFYDLSITKNVFNMLIGFVLLTILFFVIKSSYEKR
                     KGQAPKGAQSFMEPIIDFLIDDIVVKNIGPNYKKYLPFLLSVFFFILINNLLGLVPFL
                     GSINSSGNISFTMALALCSLLVINLNGTKDYWGHIFAMPGVPKWVLIILTPIELLGVI
                     LKPAVLMLRLFGNIIGGHITVLSIVSLIFIMGKMGQSMGGAAAGGAVAFPILLFVNAL
                     ELFVAFLQAYVFTLLTAVFIGAAIEEHHHAEEHH"
     gene            2827..3543
                     /gene="hisA"
                     /locus_tag="PLS16_02140"
     CDS             2827..3543
                     /gene="hisA"
                     /locus_tag="PLS16_02140"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HNJ59906.1"
                     /translation="MLIIPAIDIIDAKCVRLTKGDYDTKKTYNENPLEQAKIFEASGL
                     THLHLVDLDGAKKGQVQNWKVLETIASNTNLHIDFSGGIKTREQAQQAFDLGAKQITV
                     GSLAVKNQKEFTDWIWEFGEDKLILGADVKDKQIAIQGWQENSNQDIFSFLDYYFEEG
                     IDYVLCTDVSKDGMLQGASVDLYKELLEEYPEIKLIASGGVSCLQDLVELKAIGCYAA
                     IVGKAIYEGKISLKELKEFL"
     gene            3543..4295
                     /gene="hisF"
                     /locus_tag="PLS16_02145"
     CDS             3543..4295
                     /gene="hisF"
                     /locus_tag="PLS16_02145"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNJ59907.1"
                     /translation="MLTKRIIPCLDIKDGQTVKGVNFVDLIHAGDPIELAQRYVEQGA
                     DELVFLDITATVEQRKTFAELVSKIAKAINIPFTVGGGISTIADVDKLLNAGADKVSI
                     NSSAVKKPNLIDDLSKQFGAQCIVVAIDTKLDEDWKVYVKGGREATPLYTKDWAKEVQ
                     ERGAGEILLTSMNHDGTKQGFALDITGWCANNLKIPVIASGGAGKKEHFIDVFQKANA
                     DAALAASIFHFGEIPIPELKQFLQKNNISVRI"
     gene            complement(4292..5095)
                     /locus_tag="PLS16_02150"
     CDS             complement(4292..5095)
                     /locus_tag="PLS16_02150"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HNJ59908.1"
                     /translation="MKRIVIILFFCVIVAQTQAQTSTNKPNKPSFTDDFLNENLMNDI
                     MSQLDAFEKEVKYEDKYTFSEYSKIKISNTNKEKTSNVVYDIYLSENASMMKMNVGNG
                     NKSEDDINIIMDSKNKSMITLNEQDKSAIAINTEGLMDLANSFSNKHLSTQKNTTQVS
                     IQKTGRTKTILGYACNEYKITNSDSEGLNEIFAWVTEDPSIKNIDFFSALGKQLKLPN
                     PPKSEIIGTTLEFNGKNLKTQDTFSMQVLEISKSTYTKDLSTYKMEGGF"
     gene            5169..5762
                     /gene="hisIE"
                     /locus_tag="PLS16_02155"
     CDS             5169..5762
                     /gene="hisIE"
                     /locus_tag="PLS16_02155"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HNJ59909.1"
                     /translation="MQIDFEKMDGLVPAIVQDANTGNILMLGFMNEEAYKKTVELRKV
                     TFFSRTKNRLWTKGEESGHFLALKDIKVDCDNDTLLIKAIPNGPTCHTGKDTCWFEKN
                     NEKFSFLFDLENIIEDRKNNPSEKSYTTSLFNRGINKIAQKVGEEAVELVIEAKDEND
                     DLFKNEAADLLFHYLILLQAKGFKLEDIIEVLRQRHK"
     gene            complement(5765..6619)
                     /locus_tag="PLS16_02160"
     CDS             complement(5765..6619)
                     /locus_tag="PLS16_02160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNJ59910.1"
                     /translation="MTGFGKVLCVINNTNFSVEIKSLNSKQIEINLRNANFLRDLELE
                     LRKLLQQKLLRGKIDVSISEEINDKQSILNLDNIEQLYRELIDFGQNKNIPLGDIFPT
                     VLKLSEQNKRDSFTIEDEKKEAILETLETGIDALIEFRKKEGLELEQDIVSRIKWIQK
                     LRTNIVPFENNRIVKIRERIENELTQLKNITIDSNRLEQEIIFYAEKLDITEEHTRLQ
                     SHCTYFLEIVENNTIEKGKKLGFIAQEIGREINTIGSKANDADIQKLVVQMKDELEKI
                     KEQLNNIL"
     gene            6731..7954
                     /locus_tag="PLS16_02165"
     CDS             6731..7954
                     /locus_tag="PLS16_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ59911.1"
                     /translation="MSNQAIESPTKKIWKRLKKNKPAIFSLCVIIIALFLAIFAPVIA
                     QDKTPDADDQVLELTNLSPNTKVKMLLVRKNRLENPSSFLKTVFIGKDNPYQMIPISN
                     YEMNDTSIVVQTYAGEDRPVETKAFSLAQVVYAVSNEKPNIQYQDKKALFYDINNQFE
                     KKDISYIKADIEKYNIVTKRYLLGTDGYGRDILSRLLLGVRVSLTVGFIAVLISLSIG
                     IFMGAIAGYFRGWVDNVVMWLINVIWSIPTILLAMTIRFAIGDKINSFLAIFIAVGLS
                     MWVDTARVVRGQVLGVREMEYIQAARSLGYNHFRTIFKHILPNIIGPILVIAAADFAS
                     AILIEAGLSFVGIGVKPPTPSWGTMLNDHLAYLTTNKAFLALAPGFCIMILVLAFNLL
                     GNGLRDAFDVKGQAV"
     gene            8053..9666
                     /locus_tag="PLS16_02170"
     CDS             8053..9666
                     /locus_tag="PLS16_02170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNJ59912.1"
                     /translation="MQKTFLSLAIIFSVFVSFAQTYQSKMSSFLNLVDNYYVEKVNMD
                     SIVEVGIKSMLKGLDPHSIYYSADEYKKTNEPLEGNFEGVGIQFQIFQDTLLIVHVIH
                     GGPSQKVGLQDGDKIIYIDTAKVAGIGLDNDKVFKLLRGKKGTTVTVKVKRYGEPDLI
                     EFNIVRDKIPIYAIEAQYMATSEIGYIKLSRFSATATDEVIKAIDSLKKQGAKKLILD
                     LTGNGGGYLNQAHALSDLFLTEGKLVVYSEGRSQTRIDLNATNFGNFENGNLVIMVDQ
                     GSASASEIVAGAIQDWDRGLIIGRRTFGKGLVQKAYNLADWSVIRLTMAHYYTPSGRN
                     IQKPYGKNDGVYSEDINERYESGELFDEHKTKIIDSTKFYTNNKRVVYGAGGIIPDIF
                     VPLDTTWTSKFYSKLIRTGAFNQFALTYVNENRDILMQKYNSANKYIQSFEVDSTLYN
                     NFIAFAKTKNAEPDSAEVYKQSIPVIKNQLKGLIGQNLFDYSVYYQITNIQNPIYKRA
                     LEAFEDKSFKEHNITPAPFKKEKNKRASQ"
     gene            9773..10375
                     /gene="cysC"
                     /locus_tag="PLS16_02175"
     CDS             9773..10375
                     /gene="cysC"
                     /locus_tag="PLS16_02175"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HNJ59913.1"
                     /translation="MQNDNNIHPIFGRLLQREDKESLLKQKSICIWMTGLSGSGKSTI
                     AEGLEKKLHQNGILSMVLDGDNIRSGINKNLSFSEEDRTENIRRIAEINKLFLDCGII
                     TINCFVSPTLDIREQAKNIIGDADFNEIYINASFDECAKRDVKGLYAKALKGEIKNFT
                     GLDAPFEAPKNPSLEIKTAELSIEESIDKIYNHFINKIKQ"
     gene            10379..11287
                     /gene="cysD"
                     /locus_tag="PLS16_02180"
     CDS             10379..11287
                     /gene="cysD"
                     /locus_tag="PLS16_02180"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HNJ59914.1"
                     /translation="MALDYHLNHLRELESESIYVLREVAAQFENPCILFSGGKDSIVV
                     THLAMKAFYPANIPFTLLHVDTGHNFQEALDFRDQLVEKNGLKLIVASVQEAIDKGLV
                     REEKGYNASRNALQTVPLLEAIEKNKFDCAIGGARRDEEKARAKERFFSHRDEFGQWD
                     PKNQRPELWNIFNGRKHIGEHFRVFPISNWTEMDVWQYIYQEQIEMPNLYFSHKRMVF
                     ERDGVWYSDNPYMQKKPNEVAEEKVVRFRTIGDISCTGAVFSEASTLEDIIQEVAASR
                     TTERGTRADDKRSEAAMEDRKKAGYF"
     gene            11389..12675
                     /gene="cysN"
                     /locus_tag="PLS16_02185"
     CDS             11389..12675
                     /gene="cysN"
                     /locus_tag="PLS16_02185"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HNJ59915.1"
                     /translation="MTTTQENFDSKAYLDMELLRFTTAGSVDDGKSTLIGRLLYDSKS
                     IFEDQIEAIESASEKRGEEYVNLALLTDGLRAEREQGITIDVAYRYFSTPKRKFIIAD
                     TPGHIQYTRNMVTGASTANLAIILVDARKGIIEQTFRHSYIASLLQIPHIVVCVNKMD
                     LVDWDEATFDKIVSDYKAFASKLDIKDVTFIPISALTGDNVVERSKNMTWYNGSTLLY
                     LLENIHIGSDLNHIDARLPVQYVIRPMKNEYHDYRGYAGRVAGGVFRVGDIVKALPSG
                     FTSKIKAIKTMDGDLDEAFTPQSVTLLLEDEIDISRGDMIVRENNVPQTGQELDVMLC
                     WMNEKPMPLRGKYILRHTSKECKTIITEIKYVLDTNTLTRVEGQTELKLNEIGRVSIK
                     TTVPLFFDSYRKNRQTGSIILVDEATNETVAAGMIV"
     gene            12803..13489
                     /locus_tag="PLS16_02190"
     CDS             12803..13489
                     /locus_tag="PLS16_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNJ59916.1"
                     /translation="MRNINIIRLFSLFGLIILLSCQKNHSIPSAFVNLKKLIPNLELD
                     LRYKGNHNFMGRPVRGYEANKLYITLEAANALKKVQKELNQEGLGIKVYDAYRPQRAV
                     QNFMEWAKNIDDTIAKREFYPEVDKKDLFVLGYIAEKSGHSRGSTVDLTIIELSNKKE
                     IDMGSAFDFFGIQSHHDYTLLSEQQISNRLKLRTIMEKYGFKTIEEEWWHYTLKNEPF
                     PESYFDFPIR"
     gene            13655..14209
                     /locus_tag="PLS16_02195"
     CDS             13655..14209
                     /locus_tag="PLS16_02195"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNJ59917.1"
                     /translation="MILLSAITIIIAILIVLFLIFVYNQLTKSRILLKEAWSGIGIFL
                     QQRNDVIPSLVEIVKGYVEHENKTLVEVTKWRNKSAMATSPAEQSEASAGLNKALLDF
                     YQVSEQYPELKANENFMHLQNELTEIEVKLNNSRRYYNGTVREYNQQRAVFPQNIIAS
                     LFNFLPEEFFEEEESAKIAPKINF"
     gene            14225..16318
                     /locus_tag="PLS16_02200"
     CDS             14225..16318
                     /locus_tag="PLS16_02200"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HNJ59918.1"
                     /translation="MKRCFFISIQILILISNLYAKNISFSSETDALFNDSSYSSLIKK
                     ATFDVINVRDIDLYFNLNYAVLENEIIAISKKQIVEDLSKVSNSKRQLKTSKAYRIYR
                     QTIYDAVQKVLLKNDTLLQKLLEKNLDDQILDFQSEIHLSKDGKIRVIEKIKVANFNH
                     GNNNLIQRGITRDFPIQYCNKLGFLYSVPFHVNSITRNGEPENFFLENLTNGVRIYCG
                     QSDIILPEGIYEYIIDYTTARQLKFHNNRDEFYWNITGNGWLFKINKVKGNVFSPQTS
                     TPLDMSVYTGKQGRKESNAKIYLADSTNVEVVSTQSLLENEGLTISVSYPKETFIEPN
                     FLIQAIQLLKDNKYLFFIIFFVFIHFILLYLHWKAVGKDPKSGVIIPLFEPPNELSPA
                     SVGYINKQSFNDTLFSAALIDLAVKHKINIDVESKGIVFKSNVYSFRTNNIDQDDYCK
                     KNYGFEETDLNGITISKGTYNSKFASIYFLFKKHLDNQILKIDEKDSSITKYLSLNYD
                     KIGIGFFALFGIFIATIIYLGTYKPPANHIIFLVCLFLLAVVVQIIFTLIIKAYTNLG
                     RKTADKIQGFKIYLSTTEQDRFERMNPPEMSLQLYEKYLPYAIALNVENEWSEKFNDI
                     VEKAINNGEQPVMYRSYQSMSSSSFSSFTSSLSQGLSSSISSASTIPSSSSSSGSSGG
                     GSSGGGGGGGGGGGW"
     gene            complement(16328..17482)
                     /locus_tag="PLS16_02205"
     CDS             complement(16328..17482)
                     /locus_tag="PLS16_02205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ59919.1"
                     /translation="MFYYQWRQTETITNTENSVNTSVAIIIPARNEAQNISNLLDAIL
                     QQDFPKELIEIIVVDDCSEDETLAIVQQKLQQNTFAYKVLELKNFIDVEHLNAYKKKA
                     ISIGVEQTNQELIITTDADCIVPKNWLKKIVQFYESTNANLIASPVLLHSSTHFTFFS
                     RFQILDFCGMQLITAACIKSGIFNMGNGANLAYKRSAFKQVNGYEGINQKASGDDMLL
                     VYKIAQIDDKKVFFNKNIEATTQTEIIHNLKSFLQQRFRWTSKAMDYQDKRMTMMLGL
                     VLLFCVSMVVNFAMIIGIILYYQIFPNHTIVGYLQFLSVLFFLIVFSFQFVLKTIADY
                     ILLNEASSFFNEKRQLKGFIISEILHIAYIVYVGIVGNFVSVKWKDRAVK"
     gene            complement(17556..18578)
                     /gene="pheS"
                     /locus_tag="PLS16_02210"
     CDS             complement(17556..18578)
                     /gene="pheS"
                     /locus_tag="PLS16_02210"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNJ59920.1"
                     /translation="MNLFEQTQQLLEDVKAFPIASAQELEQFRIKFLGTKNVLKDIFA
                     QIKAVPNEQKKEFGQLVNQVKLVAEEKFATAKVQFSTTQQTASNIDLTLPGEHLPIGS
                     RHPVNIVKSQIISIFNKIGFSIALDREMEDDWHNFTALNMPEDHTARDMQDTFYLQVN
                     PAMLLRTHTSSVQTRLMEQQKPPIRIIAPGRVFRNETISARAHCFFHQVEGLYVDENV
                     SFADLLQTLDFFVKEMFGKETKIRLRPSYFPFTEPSAEVDVSCFICNSKGCNVCKHTG
                     WVEILGCGMVDPAVLENCGIDSNKYTGFAFGMGIERITMLKYQINDLRLFSENDMRFL
                     KQFQSV"
     gene            complement(18647..19732)
                     /locus_tag="PLS16_02215"
     CDS             complement(18647..19732)
                     /locus_tag="PLS16_02215"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HNJ59921.1"
                     /translation="MRKNKLILLLSLMSFVVLFSCKRDDNAKNDRGRTLNRILFGSCS
                     SQHNDIVGIYNYMGAQYPDLYLAMGDNIYGDFFAILPGTYDYMRMAYENMFAKPEVKR
                     FYDKVETLAVWDDHDFGQNDGVIDNTAKYYAKTLLFDNFKVAPDDPRRFNPSGEIYAN
                     YEYGDEAHRVQIILLDNRWNRTPYAAPGPLSALSGYDSIVTPDARMMSDEQWAWLEAQ
                     FKRPAKLRFVVSGTQFSASYNGGEAWSVMPNEKRRMVELIKSTRVNGLAFLSGDVHFG
                     DENVLKVPDCYPIFDFTSSGLTHYTSSPYASTNRIKGSGAPYAGLNFGQIDIDWNKTP
                     IEYTVTIRNSTAKPVITRKISVADISF"
     gene            19865..20497
                     /locus_tag="PLS16_02220"
     CDS             19865..20497
                     /locus_tag="PLS16_02220"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNJ59922.1"
                     /translation="MIAKISTNLDRKLPGTSAHKKLQPYYPSAKNLSIPTNPFAKKSA
                     VLVLLFPKNNQINVVFIERPANTGVHSKQISFPGGGREKNDKNYTATALRETYEEIGI
                     EASKIQVLGKLSKLYVVASNYLIQPIVGFINEVPTYRISKNEVDNIIEMPLVDLIQAE
                     IMEKPIHSAMGITLQAPYYDLEGRTLWGATAMITSELVDLSTDILKNIEL"
     gene            complement(20502..21746)
                     /locus_tag="PLS16_02225"
     CDS             complement(20502..21746)
                     /locus_tag="PLS16_02225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ59923.1"
                     /translation="MAQQGKINTLFLSYDGMTDSLGQSQVLPYLVGLSKHNYHITIIS
                     FEKEEVFEKNKATIQQIADTNQIIWQPLKYTKKPPVFSTIYDVYWLKKLVKKQIKANN
                     IQIIHCRSYITALIGLWAKKQFGTKFIFDMRGFWADERVDGKIWNIEHPLYRKIYQFF
                     KKKEIEFLQNADYTISLTHNAKNEILSWENFTKNKPRIEVIPCCVDTNLFSKEKVDIQ
                     QVENWKQKLQIQDSDFVLTYLGSVGTWYMLDEMMAFFAQLKSKITNAKFLFINKDEHQ
                     HILEVAKKYNVEDSVILQGGNRTEVPALLSLSAISIFFILPSYSKKASSPTKQGELMA
                     LQIPIVCNAGVGDTDFVVERYHSGFIVRDFKYDAIIEQIHQEHWHQIFDLDKIRNGAI
                     DYFSLANGIEKYANVYTKVLSS"
     gene            complement(21748..23151)
                     /gene="rlmD"
                     /locus_tag="PLS16_02230"
     CDS             complement(21748..23151)
                     /gene="rlmD"
                     /locus_tag="PLS16_02230"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNJ59924.1"
                     /translation="MRKVQKIEVLKIDKIAAEGNGIAKTEQGIYFVEKAIPGDVVEAK
                     IIKKKKDYGLAKIQKILEPSKERIDAVCSHFGTCGGCYWQSVNYTTQAEFKQQIVLDT
                     FWKIAKIKIENPLPILAAQQIFEYRNKMEYTFSNRAWLTDEQIQSDEVFEKRALGFHV
                     AGSFASILQIDKCYLQDDFANTIRNHIYQFCVDNNFEFYNLKLQEGFVRNLIIRNTTL
                     GEWMITVCVAQANQKQIDLLMQDIQTNFPKIISLNYVINTKKNDSIYDQEVINFSGRP
                     YIIEVLDGIKYKISTKSFFQTNSFQAKILYDVTVKFAKIEPNDIVYDLYCGTGSIALY
                     VAKYCKQVVGIEQIDDAIIDARENARMNQITNAKFYSGTVEKLLDEEFIQENQMPDIV
                     ILDPPRAGLHNQVVETLLIAKPKRIVYVSCNPATQARDVQLLSAQYNLLESQAVDMFP
                     HTYHIENVVLLELKEEA"
     gene            23253..25178
                     /locus_tag="PLS16_02235"
     CDS             23253..25178
                     /locus_tag="PLS16_02235"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNJ59925.1"
                     /translation="MKKSLLVFFLGFTLFSSKAQDDVLFTIGNEPVKLSEFEYIYNKN
                     NFSGANKYSKKSVDDYLDLYINFRLKLKEATELGLFNNNKFKEEFATYENQLINNYVE
                     KEVLDKVLLQEYERSKTDVSLSHIFVPFYNDSTKTIADKKIKAIYEKIKSGKSSFEIA
                     AKESEDKNTNTKDGYLGWFNSYQIILPELENVAYTLKPKEISAPFQSKYGYHILKLND
                     TRPARPMLRVAIIKKNILNQNDPASVQALRDTMQKIYHLFKSGTPFNDLVQNYTDDEN
                     SKQFGGKIDWFGIHYYTPVFEEAAYSIKKIGDISEPVETKLAFYLIQKIDETKPKSFE
                     EEKTNLRSKLLVSDIYEQAMQSFLNTQKSKYNFIAFNNNIYKFKQHLNNLIGNTAFKY
                     KDTIPNNILYKIGNQDFDQNKIGKAIEKIYYSYAPRNTADSRIDVLYDEIEKENILAQ
                     VKADIRSNNEEYKQLINEYKNGIMIFDLSEQKIWNKASSDTAGLKQFYNTHAADFLQK
                     ATYKERNITISQSKSAKKIYKIILSNPNISNNVLKDKLSLLDEKNTTIDEITHTVSSN
                     NAIDMSIKKPIKKEDKFVITQQYQYTPQQPKPYDEIKGYVIAAYQEQLEENWINDLKA
                     KYPVKINEPILNKIIKH"
     gene            25228..26568
                     /locus_tag="PLS16_02240"
     CDS             25228..26568
                     /locus_tag="PLS16_02240"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNJ59926.1"
                     /translation="MIRILVLTFLFGLISNNLNAQNGIVLDKIDAIVADKIILQSDIE
                     NQMEIMNLRDENAAENKCDLMYQMIFNKILTVQAEKDSLPIAESDVEAELDRKINYFI
                     SMAGSVEAFEAYYNKNVQQIKDDFRDDIREQMYADEMRRKIVGELKVTPSEVKTFFEN
                     IAPEDIPYFNAEVELGQIVVKPKISDEQRQIALNKINDIKKRLTEGESFDLLAGLYSE
                     DPGSAQEGGDLGWANRGTFVKEFEAVAFKLKPEEISDVVETTFGFHIIQLIERRGDQI
                     NLRHILIRPKSTSAGAELAYKRIDSIRTAILDNKLAFSEAVKHFSEDEQSKNVGGYLI
                     NQETGSTTNEVKDLDPEMYKAIENLKIGDITEPQMFQSGDGSPAYRILKLFSKTEPHV
                     ANLQQDYDRIQSAALNQKEQGVVEKWLQKNLGKTYLMLDPKYKSCENLKDWVKE"
     gene            26578..27543
                     /locus_tag="PLS16_02245"
     CDS             26578..27543
                     /locus_tag="PLS16_02245"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ59927.1"
                     /translation="MQKFTSDTEGIQLLAKDYQSLKNEIHKVIVGQDKVIQFVLLSIF
                     SDSHSLLIGVPGLAKTLLVKTVADTLDLDFKRIQFTPDLMPSDIIGAEVINENRQFQF
                     NKGPLFANIVLADEINRTPPKTQSALLEAMQERQITTAGHQYNLDLPFFVLATQNPIE
                     QEGTYPLPEAQLDRFMFSIQLDYPSYEEEVAVVKNTTTDQKIVTKKVITKADIIAYQH
                     LIRRMPIADNVLEYAVKLTSSTRPNTSNTPKIIQDFVAWGAGPRASQALVLGAKTHAA
                     INGKYSPDIEDVQNVALPILQHRVIRNFRAEAENISIEKIIKDLL"
     gene            27601..30750
                     /locus_tag="PLS16_02250"
     CDS             27601..30750
                     /locus_tag="PLS16_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNJ59928.1"
                     /translation="MNKNSDLKIFKASAGSGKTYTLAKEFVKILIVQPYHYKNILAIT
                     FTKKATAEMKSRILAFLKEIKEDKNSKLTLEIIEEIKEEKAVDVSQNIQEQATKAINL
                     ILHDYGHFQVTTIDSFFQLIIRSFAKELKLPIGMQIEMDTDSVLHNSVIQLLEESETE
                     NEIAQWLEEYALNNIDNDKGWKIEKNIFTLSQELLKEEFLLLNENFEQIRQPDFSKYK
                     AIADEQQNIINIYKKNIQNKAKLAEEIIKESGINAKLCSRNCLNSFIKNSKELSFDSY
                     KTIRNVVFENANLVSNDNAKNYVEATAKIMSVWDNQLKVIIEDIINHHEKHISNYITA
                     RSIQKNIYSMALLDALSIKISEYRSQENVILITDASFFISKIADTEATPFIFEKTSSF
                     INYILIDEFQDTSTLQWKSFMPIVIEILSKGYGLALIVGDPKQSVYRWRGGKMELIIK
                     KVDDDLNNFTSLKKEKTLRNNFRSLTNIIDFNNVFFRTTIETYKDFELLSKAYHEQEQ
                     TQTEEVKAKKNGGLIRCDWIESSKNKKDSTLEDDEEDKETENKILIVLTERLYELNKK
                     TNWKDVAILVRTNKEGIKIANYFQQLEKPIPFVSGESLLIKNNLNIQLIIATLEYFIS
                     DEVFYKYKLENFYCNYIGIKNYDEILYHKQSFEFEKRFKEFKISKHIKNNKLIDIVHT
                     LLHIYEMDKESNAYIVRLLDDIYKFSQNKGHNIISYLYYWEEKKDKISVLPSETTDAV
                     KIITIHKAKGLEYPIVILPFANWSLMPKANTIFWAKSEVFKTEGQVMPLSYVKNLEQS
                     EFKTAYLKEKEQSIIDNINLLYVAFTRAEKELHIFCKQKKANPDEAKSINDIITNTLN
                     NAFSENFVTDKSFILGTSANFEVKEKENNDANNLKLASINTNQNFQLLSSSYSDENTK
                     KGDIIHHLVNNIHSLKMNKLDLYSIQRQYLLDDEAIVYYKNKLIEIFKLFIEKGFIQA
                     ENTYLYEKNFYYKNKVLRADLVIITPDENIIIDYKTGAEEDKHKTQINTYKKAYELMN
                     KQKTKAFLLYTESMTLKEI"
     gene            30752..31711
                     /locus_tag="PLS16_02255"
     CDS             30752..31711
                     /locus_tag="PLS16_02255"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HNJ59929.1"
                     /translation="MHFIKKYIEESFEIIELSNQFINAKIVVQQGFNLFSLVYKNIET
                     IYFDKTLIENFQDRKLAGIPFMHPWANRLDRDKIFNRSFTMNDILNAVLYRDGNNLAL
                     HGLILKSDKWQIIDMSATDLKASCTAKLAFNETHLLDLFPFKHNIFITLELESEKLFY
                     KVEIENLSSNEMPICFGFHPYFNIKHSENNILIVPDAEVALVDEKLIPTNKYDEKEAL
                     FSFKENQIPIKNQNIDHAFLHQNQNENYQLKTDNYLVTFNLDENYTVVQIYHPNNEQK
                     PYICIEPMTALANALNTGNYNTIAPNSKFNAVFSIEYCSTIEH"
     gene            complement(31779..31851)
                     /locus_tag="PLS16_02260"
     tRNA            complement(31779..31851)
                     /locus_tag="PLS16_02260"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31816..31818),aa:Lys,seq:ttt)
     gene            32381..32626
                     /locus_tag="PLS16_02265"
     CDS             32381..32626
                     /locus_tag="PLS16_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNJ59930.1"
                     /translation="MEVQVINIGNSKGIRLSKSILEQYNISDTLDLILEKGRIILKPK
                     SVPRKGWEKSFKLMHANGDDQLLIDDIFEDETFGEWK"
     gene            32617..32940
                     /locus_tag="PLS16_02270"
     CDS             32617..32940
                     /locus_tag="PLS16_02270"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HNJ59931.1"
                     /translation="MEIKQYEIVLVNLDPTIGSEIKKTRPCAVISPNEMNKYLQTIVV
                     APLTSSSKPYPTRVEIKQAKTKGWIVLDQIRTVDRKRIFKKLGTLTKIEIEAVKNVIM
                     ETYVE"
BASE COUNT        12132 a         4884 c         5081 g        11147 t
ORIGIN      
        1 tttacttttt taagtttttg ctttacagaa ttatctaaaa ttttatctcc aaaatcaaca
       61 ataaaaccac ctaaaataga agcatctatc ttttgattga tttttacatt ggctttccca
      121 gattcctttt taatataatc ttcaattttt ttctgtgtat cagcatctaa ttctgacgct
      181 accgtaacat ttacttctgc aattcctttt atttggttgt atttgctgaa aaatttggat
      241 aaaattaaag gcaaatattg ttctctttta ttattaataa taatatctaa gaattgcata
      301 gtgcttgcct gtacttttga ttcaaaaatc tctttaaatg ctaccaattt tttagaagaa
      361 tctataattg ggctttttag atagttacta aattcccgac tttcagcaat tgcaccagcc
      421 accacttgta agtctgcata agattgctct aacttatttg tagaagtaga ttccgaaaat
      481 atagcatctg cgtatctatt ggctaatttt tctgctgaca ttagttgtta tttatatttt
      541 ctactaatga attaatatat tgctgatgat ctttggaagt gcttagttct ctgttcaaaa
      601 ttttctcagc aatttcaact gaaaattggc taagttcatt tttaacctct tctaaagctt
      661 tcttcttttg agtatcaatt tcagcttttg catcggcaat gatttttgca gcttcatctt
      721 tagcacgatt atgtgcatca gcgataacag actctttaat gtcctttgct tcttttagaa
      781 ttttagcact ttcttctcta gcttctttta ataaatttgc attgtctgct tgtaactgcg
      841 acatttctgc cttagctttt tcagctgcgt tcaatgcatc gttaatagat gcttctctat
      901 ccttaatagc gttagctatc ggacctaaag ctttcttacc aactataaac caaaatacta
      961 ggaaaataat agttgtccaa aacaccaagc ctaaatcagg tgtcaataaa gataatgtat
     1021 caaattttaa aagcataaag cataaaattg aattacaata ataatattgt aattagattt
     1081 aataattatt ctaactcttg tattagttca tcaagaatga aagaatcata ccgatgattg
     1141 ctaccccttc aataagagct gctgttaaga tcattgctcc tctaatatcg ttagctgcct
     1201 ctggttgtct tgcaatactt tgtactgagt tacttccgat taaaccgatt ccgattcctg
     1261 ctgctaatgc tgctaatcct gcgcctaatg ctgcaaatcc tgtcataatt taaaatttaa
     1321 ttgttttgtt aataatttta ttcttaataa ttaatgatgt tcttctgcgt gatgatgttc
     1381 ttctattgct gcacctataa atactgcagt caataatgta aatacataag cttgtaagaa
     1441 tgcaacgaat aattctaatg cattcacaaa taataatatt ggaaatgcaa cggctccacc
     1501 agctgctgct ccacccatac tttgtcccat ttttcccatt ataaaaatca gagaaacaat
     1561 acttaatact gtaatatgac ctccaataat atttccaaat aaacgcaaca ttaaaacggc
     1621 aggtttcaat attacgccta ataattcaat tggtgtcaaa ataatcaata cccattttgg
     1681 aacgcctggc attgcaaaga tatgtcccca ataatctttt gtaccattta agttaataac
     1741 caataatgaa cacaatgcca aagccatagt gaaagaaata tttcctgatg agttgatact
     1801 tcctaaaaat ggaactaatc ctaaaaggtt attaattaat ataaaaaaga atactgacaa
     1861 taaaaatggt aaatattttt tatagtttgg tccaatattt tttacaacaa tatcgtcaat
     1921 taagaaatca atgataggct ccataaaaga ttgtgctcct ttgggtgctt gaccttttct
     1981 tttttcgtaa gaagatttga taacaaagaa caatatagtc aatagtacaa atccaatcaa
     2041 catattaaaa acattctttg taattgacaa atcgtagaat gtatctcttt ttcctgtttg
     2101 agcatctact actcttgaat gttccatttt atatccatca ataatacctt ctggtgcatg
     2161 atgaaatgca gaagacataa agaaagatac ttttttagtt tctttattaa ttaaaataca
     2221 tggcaacgga atagtaaggt gtgtatgacc catagttccc aaatgatttt catttgcatc
     2281 agaaacgtgg tgtaaaattg tctctacaat gtctgtctcc tctttttcta cttcttcgtg
     2341 agcaatactt ttttcattaa ttagcataat gccttcattc gcaacagtat ctgtattggc
     2401 taatgctgga tttatagcaa aagttaaaga aaataaggct aaaaatatat atttcaattg
     2461 agttctgaac acggtttcca aaaattattc ggcaaagata gtaaattgaa gttatttata
     2521 ataaaaaatt tagaattaaa tgtttgttaa gcgattgtag aaaataagaa attttggaat
     2581 tttagtaatt caataattat taaaaaaatc taaagcatct atttacaatt tctataataa
     2641 aaataggctt atgcacttgt tccttttttt ataattatat acctaaccat cttaacacaa
     2701 caaaaagtgt gtcatttcaa aaataaagca aaaagaaaac actctacttt ccatttaagg
     2761 gaatttttct aaactttata agaacaatac tattttaaac cttacttttg cagcatattt
     2821 atttttatgc taattattcc tgcaattgat attattgatg ccaaatgtgt gcgactcaca
     2881 aaaggtgatt atgatactaa aaaaacttac aatgaaaatc cacttgaaca agcaaaaata
     2941 tttgaagctt caggcttaac acatttacat ttagtagatt tagatggtgc taaaaaagga
     3001 caagtgcaaa actggaaagt tttggaaaca atagcctcta atacaaactt acatattgat
     3061 ttttctggtg gtattaaaac tagagaacaa gcacaacaag catttgatct tggagcaaaa
     3121 caaataactg tgggcagttt ggctgttaag aatcaaaagg aatttacgga ttggatttgg
     3181 gaatttggtg aagataaatt gattttagga gcagatgtta aagacaagca aatcgcaatt
     3241 caaggctggc aggaaaattc aaaccaagat attttttcct ttttagatta ttattttgaa
     3301 gaaggcattg attatgtttt atgtactgat gtcagcaaag atggtatgtt gcaaggagct
     3361 tcggtagatt tgtacaaaga actactagaa gagtaccctg aaataaaatt aatcgcatct
     3421 ggtggagtct cttgtttaca agatttagta gaattaaaag ctatcggttg ttatgccgcc
     3481 attgttggta aagcaattta tgaaggcaag attagtttaa aagaattaaa agaattttta
     3541 taatgcttac aaaaagaatt attccttgtt tggatataaa agatggtcaa actgtaaaag
     3601 gtgtcaattt tgtagattta atacacgctg gcgaccccat tgaattggct caaagatatg
     3661 tagaacaagg tgcagatgag ttagtttttt tagatataac tgcaacagtt gaacaacgca
     3721 agacttttgc tgaattagtt tctaaaattg caaaagcaat caacattcca tttactgtcg
     3781 gcggtggtat ttctacaatt gctgatgtcg ataagctatt aaatgctgga gctgataaag
     3841 tttctatcaa ttcatcggca gtaaaaaaac cgaatttgat agatgattta tccaagcaat
     3901 ttggtgctca atgtattgtt gtggcaattg atactaaatt agatgaggat tggaaagtat
     3961 atgtaaaagg cggaagagaa gcaactccat tatatactaa agattgggca aaagaagtac
     4021 aagaacgtgg tgctggcgaa attttattaa cttctatgaa tcacgatgga accaaacagg
     4081 gttttgcatt agatattacg ggctggtgtg ctaataattt aaaaattcct gttattgcaa
     4141 gcggtggagc tggaaaaaaa gaacacttta tcgatgtctt tcaaaaagca aatgctgatg
     4201 cagctttagc tgcttcaatc ttccactttg gcgaaattcc aattcctgag ctaaaacaat
     4261 ttcttcaaaa aaataatatt tctgttagaa tttaaaaacc tccttccatt ttgtaagtac
     4321 ttaaatcttt agtataagtt gattttgaaa tctcaagaac ttgcatagag aaagtatctt
     4381 gcgtttttaa atttttacca ttaaattcta aagtagtccc aataatttca ctttttggag
     4441 gatttggcaa tttcagttgc tttcctaatg cactaaaaaa atctatattt ttgatacttg
     4501 gatcttctgt aacccaagca aatatttcat ttaagccttc actatcacta ttggtaattt
     4561 tatattcatt acatgcataa cccaaaatag tcttggttct tcctgttttt tgaatactta
     4621 cttgcgttgt atttttctga gtagaaagat gtttattaga aaatgaattt gccaaatcca
     4681 tcaaaccttc ggtatttatt gcaatcgcag atttatcttg ttcatttaag gtaatcatag
     4741 atttgttttt ggaatccata attatattaa tatcatcttc agatttattt ccatttccaa
     4801 cattcatttt catcattgat gcattttctg aaaggtaaat gtcataaacc acattacttg
     4861 ttttttcctt attggtattt gaaattttaa tttttgaata ttcactaaac gtgtatttat
     4921 cttcatactt aacctctttt tcaaatgcgt caagctgtga cattatatca ttcataaggt
     4981 tttcgttcag aaaatcatca gtgaacgacg gtttattggg tttatttgtt gaggtttgtg
     5041 cttgagtttg agctacaatt acacaaaaaa ataaaattat tactatcctt ttcatagttg
     5101 cctcgttata cttttaaagt tataagatat taatgaaaaa acaaaacttt atttagtatt
     5161 tttgcccaat gcaaatagat tttgagaaaa tggatgggct tgttcctgca attgtacaag
     5221 atgcaaatac aggaaatata ttaatgttag gctttatgaa tgaagaagcc tacaaaaaaa
     5281 cagttgaatt aagaaaagtt acttttttct caagaacaaa aaataggctc tggacaaaag
     5341 gtgaagaaag cggtcatttt ttggccttaa aagatataaa agtagattgc gacaacgata
     5401 cattattaat taaagcaatt ccaaacggac caacttgcca cacaggaaaa gacacttgtt
     5461 ggtttgaaaa aaataatgaa aaattttctt tcctttttga tttagaaaat ataattgaag
     5521 atagaaaaaa caacccttca gaaaaatctt atacgacttc tcttttcaat agaggtatca
     5581 acaagattgc tcaaaaagta ggagaagaag ctgtggaatt agtcattgaa gctaaagatg
     5641 aaaatgatga tttattcaaa aatgaagcag cagatttatt gttccattac ttgattttat
     5701 tacaagctaa aggtttcaaa ctagaagata tcattgaagt tttaagacaa agacataaat
     5761 aaaatcacaa aatattattc aattgttctt tgattttttc taattcatct ttcatctgaa
     5821 caactaattt ttgaatatca gcatcatttg cttttgagcc aattgtatta atttctctgc
     5881 caatttcttg tgctataaac cctaattttt ttcctttttc aatagtatta ttctctacta
     5941 tttctaaaaa ataagtacaa tgcgattgca agcgtgtatg ttcttctgtg atatctaatt
     6001 tctcagcata aaaaataatt tcttgttcta aacgattaga atctattgtt atatttttta
     6061 attgagtcaa ttcattttca attcgttctc taattttaac tatcctatta ttttcaaatg
     6121 gaactatatt tgttctaagt ttttgaatcc attttatacg gcttacaata tcttgctcca
     6181 attctaatcc ctcttttttt ctaaactcaa ttaaagcatc aataccagtt tctaaagttt
     6241 ctaaaatagc ctcttttttt tcatcttcaa ttgtaaaact atctcttttg ttttgctcac
     6301 ttaattttaa aaccgttgga aaaatatcgc ctaatggaat atttttattt tgaccaaaat
     6361 ctatcaattc acgatacaat tgttcaatat tatctaaatt tagaatactt tgcttatcat
     6421 taatttcttc actgatgctg acatcaattt ttcctctcaa taatttttgt tgaagtagtt
     6481 ttctgagttc caattcaagg tctctcaaaa aattagcatt tctaaggttg atttctattt
     6541 gtttgctatt tagcgatttt atttcaacag aaaaattggt attattaatg acacacaaaa
     6601 ctttaccgaa accggtcata gaatataaca tacaaaaatg attggttgga cgcaaattta
     6661 ctgttttacg aatacttatt ctaaggaata aaaaataatt gtatcttgct ttgcaaaatt
     6721 ggtgtaaaga atgagtaatc aagcaataga atctccaact aaaaaaatat ggaaacggct
     6781 aaaaaagaac aagcctgcaa tttttagttt atgtgtgatt ataatagctt tatttcttgc
     6841 catttttgct cctgttattg ctcaagacaa aacacctgat gctgatgacc aagttttaga
     6901 attaactaat ttgtcgccta atacaaaggt aaaaatgctt ttggttcgca aaaatagact
     6961 agaaaatccg agttcctttt taaaaaccgt ttttattgga aaagataatc cttatcaaat
     7021 gattccaata agtaattatg aaatgaatga tacgagcatt gttgtacaaa cttatgctgg
     7081 agaagatagg ccagttgaaa caaaagcctt ttcgttagca caagtagtct atgcggtttc
     7141 aaatgaaaag ccaaacattc aatatcagga taaaaaagca ctcttttatg atattaataa
     7201 tcaatttgaa aagaaagata ttagttacat aaaagcagat attgaaaaat acaatatcgt
     7261 aacaaaacgc tatttattag gaactgatgg ttacggtcgt gatattttaa gtcgattatt
     7321 gttaggtgtt cgagtatcac tcacagttgg ttttattgca gttttaattt ctttgtctat
     7381 cggtattttt atgggtgcca ttgctggtta ttttagaggt tgggtggaca atgtggtaat
     7441 gtggctcatc aatgtaattt ggtctatccc aacaatttta ttagcaatga ccattcgttt
     7501 tgctattggc gataaaatca attctttctt agctattttt attgctgttg gtttgtccat
     7561 gtgggtggat acggcgagag tcgtgcgtgg tcaagttttg ggcgtgcgtg aaatggaata
     7621 tattcaagcc gcaagaagtt taggctacaa tcattttaga acaattttta agcatatttt
     7681 acctaatatt attggtccta ttttggtaat cgcagcggca gattttgctt ctgcaatatt
     7741 gattgaagcg ggtttgagtt ttgtaggaat cggcgttaaa cctcccactc caagttgggg
     7801 aactatgctc aatgaccatt tggcttacct aaccacaaat aaagcatttt tagctttagc
     7861 tcctggcttt tgcattatga ttttggtgtt ggcattcaat ttattaggca acggacttag
     7921 agatgcattt gatgtgaaag gacaagcagt gtaatagctt atctaattat aatttctatt
     7981 tatttaacaa tttttgatgt agtttgccat atttaggtat ttattttgta atatcattct
     8041 aaattcgtga gtatgcaaaa aacctttctt tccttagcta taatttttag tgtttttgtg
     8101 agtttcgctc aaacctatca atccaaaatg tcttcatttt taaatcttgt tgataattat
     8161 tatgtcgaga aagttaatat ggactctatt gttgaagtcg gcatcaaatc tatgttaaaa
     8221 ggactagacc cacattctat ttattattct gccgatgaat acaaaaaaac gaatgaacca
     8281 ttagaaggaa attttgaagg tgttggtatt caatttcaaa tttttcaaga tactttatta
     8341 attgtacatg ttattcatgg cggtccatca caaaaagtag gattgcaaga tggtgataaa
     8401 attatttata ttgatacggc taaagttgct ggaattggct tggataatga taaagttttt
     8461 aaattattaa gaggcaaaaa aggtaccact gttactgtaa aagttaaaag atatggcgag
     8521 cctgatttga ttgaatttaa tattgtgaga gataaaattc ctatttatgc aattgaagca
     8581 caatatatgg caacatcaga aattggatat atcaaactct ctagattttc tgctaccgca
     8641 actgatgaag ttattaaagc cattgatagt ttaaaaaaac aaggtgccaa aaaattaatt
     8701 ttagatttaa caggcaatgg cggaggctat cttaatcaag cacatgcttt atccgatttg
     8761 tttttaacag aaggtaaatt agtggtttac tctgaaggac gttcacaaac aagaatagat
     8821 ttgaatgcta ctaattttgg aaattttgaa aatggaaatt tagtcattat ggttgatcaa
     8881 ggttcggcat cagcatcaga aattgttgct ggagcaatac aagattggga tagaggctta
     8941 atcatcggac gcagaacatt tggtaaaggt ttagttcaga aagcttataa tctcgcagat
     9001 tggtcggtaa tacgattaac tatggctcat tactacacac caagtggcag aaatatccaa
     9061 aaaccttatg gaaaaaatga tggtgtatat agtgaagata ttaatgaacg atatgagagt
     9121 ggggaattat ttgatgaaca taaaacaaaa attattgatt ctacaaaatt ctataccaat
     9181 aataaaagag ttgtttatgg tgcaggtggt atcattcctg atatttttgt gcctttagat
     9241 actacttgga cctctaaatt ctatagtaaa ttaataagaa ccggagcttt caatcaattt
     9301 gcattaactt atgtgaacga aaatagagat atacttatgc aaaaatataa ttcagcaaat
     9361 aaatatattc aatcatttga agtagattcc actttgtaca ataattttat tgcatttgca
     9421 aaaaccaaga atgcagaacc tgattctgct gaagtttaca aacaatctat tcctgttatt
     9481 aaaaatcaat taaaaggctt aattggacaa aatttatttg actattctgt ttattatcaa
     9541 attacaaaca ttcaaaatcc aatatataaa agagcattag aagctttcga ggataaaagt
     9601 tttaaagaac acaatattac accagctcct tttaaaaaag aaaaaaacaa aagagcaagt
     9661 caataaaaca agctccaatt ttgaaaaata agttaaaata aaaattaata gtctatatag
     9721 tcaatatact attggaagtt taagaaattt atttatcttt gtacctcaaa ttatgcaaaa
     9781 cgataacaat atacatccaa tttttggcag attattgcaa cgagaagata aggaaagctt
     9841 attgaagcaa aagtcaattt gtatatggat gacaggttta tctggttctg gaaaatctac
     9901 gattgcggaa ggcttagaga aaaaactaca tcaaaacggc atactttcta tggtcttaga
     9961 tggcgataat attcgttcag gcatcaataa aaatcttagt ttttctgaag aggataggac
    10021 tgaaaacatt agaagaattg ccgaaatcaa taaactattt ttagattgtg gcattattac
    10081 catcaactgt tttgtatcgc caactttaga tattcgtgag caagcaaaaa atatcattgg
    10141 agatgctgat tttaatgaaa tttatatcaa tgcctctttt gatgaatgtg caaaaagaga
    10201 tgttaaaggc ttatatgcta aagcattaaa aggagagatt aagaatttta ctggattaga
    10261 tgctccattt gaagctccaa aaaatccaag tttagaaata aaaacagcag aactttctat
    10321 tgaagaaagt attgataaaa tatacaatca ctttataaat aaaattaaac aataaattat
    10381 ggcattagat taccatttaa accatctcag agaattagaa tcggagtcga tatatgtgct
    10441 tcgtgaagtt gctgcacaat ttgaaaatcc ttgtattctt ttttcaggtg gaaaagattc
    10501 aattgtagtt acacatttgg caatgaaggc attttatcca gctaatattc ctttcacact
    10561 tttacatgtt gatacaggac ataattttca agaagcctta gattttagag atcaattagt
    10621 agagaaaaat ggtttgaaat taattgttgc ctctgttcaa gaagcgatag acaaaggatt
    10681 agtaagagaa gaaaaaggat ataatgcatc aagaaatgcc ttacaaacag ttcctttatt
    10741 agaagcaatt gaaaaaaata aattcgattg tgcaattggt ggagctcgaa gagatgaaga
    10801 aaaagctaga gcaaaagaaa gatttttctc acacagagat gaatttggac aatgggatcc
    10861 aaaaaatcaa agacccgaat tatggaatat tttcaacgga agaaaacata taggcgagca
    10921 ttttagagtt ttcccaattt caaattggac agaaatggat gtttggcaat atatttatca
    10981 agaacaaata gagatgccta atttatattt ctctcacaaa agaatggtat ttgaaagaga
    11041 tggtgtttgg tattctgaca atccatatat gcaaaagaaa cctaatgaag tagcagaaga
    11101 aaaagtagta cgttttagaa ccattggtga tatttcttgt actggagctg tattttctga
    11161 ggcttcaacc ttagaagata tcatccaaga agttgccgca agtagaacca ctgaaagagg
    11221 tactagagcc gatgataaac gaagtgaagc agctatggaa gatagaaaaa aagcaggcta
    11281 tttttaatca taaaagtagt aagaaataag acataagtat taagactgtt tttatatcaa
    11341 gtaattgaga aataaggaat aataaaagaa actttagaag aaaataatat gacaacaaca
    11401 caagaaaatt tcgatagtaa agcatattta gatatggagt tattgcgttt taccacagca
    11461 ggctctgttg atgacggaaa atctacttta atcggcagat tattatacga tagtaaatct
    11521 atttttgaag accaaattga agccattgaa tcagcttctg aaaaaagagg cgaggaatat
    11581 gtcaatttag cacttttaac tgatggtttg agagctgagc gtgagcaagg tattaccatt
    11641 gatgttgctt atagatattt ttctactcca aaacgtaaat ttattattgc tgatacacct
    11701 ggacatattc aatacactag aaatatggtt actggtgcaa gtactgccaa tctagccatc
    11761 attttagttg atgcaagaaa aggtattatt gaacaaacat tcagacattc ttatattgct
    11821 tctttattac aaattccgca tattgttgtt tgtgtcaata aaatggattt ggtagattgg
    11881 gacgaggcta cttttgacaa aatcgttagt gattataaag cttttgcttc aaaattagat
    11941 ataaaagatg ttacttttat tccaatttca gcattaacag gcgataatgt agtggaaaga
    12001 agcaaaaata tgacttggta caatggctca actttattgt atctattgga aaatattcat
    12061 attggctcag acttaaacca tatagatgca agacttccgg tacaatatgt aatccgtccg
    12121 atgaaaaatg aataccacga ttacagaggt tacgcaggga gagttgcagg cggtgttttt
    12181 agagttggag atattgtaaa agctttacca agtggcttta cttctaaaat aaaagcaata
    12241 aaaacaatgg atggcgattt agatgaagca tttacaccac aatccgttac ccttttgtta
    12301 gaagatgaaa ttgatatttc aagaggcgat atgattgtta gagaaaacaa tgtccctcaa
    12361 actggtcaag aattagatgt aatgttgtgc tggatgaatg aaaagccaat gccattacgt
    12421 ggaaaatata tcctaagaca tacttcaaaa gaatgtaaaa ccatcattac cgagataaaa
    12481 tatgtattgg ataccaatac acttactaga gtagaaggtc aaacagagct aaaacttaac
    12541 gaaattgggc gtgtaagcat taaaacaacc gtacctttat tttttgatag ttatagaaaa
    12601 aatagacaga ctggaagtat tattttagta gatgaagcga ccaatgaaac cgttgcagca
    12661 ggtatgattg tgtaaagact aaattcattt tttatcaagc caaactaaaa agtttggctt
    12721 tttttattat tctttgaaaa aatgatattt ccatttttaa attgatttac tttgtaagta
    12781 taaaaaaata aaatcctaaa caatgcgtaa tattaatatt atcaggttat tcagtttatt
    12841 tggcttaata atattattat cctgtcaaaa aaatcattcg ataccatctg catttgtaaa
    12901 tttaaaaaag ctgataccta atttagaatt agatttacga tataaaggca atcataattt
    12961 tatgggacga ccagtaaggg gttatgaagc aaataaacta tacattactt tagaagcggc
    13021 taatgcctta aaaaaagttc aaaaggaact aaatcaagaa ggtcttggta ttaaagttta
    13081 tgatgcctat cgacctcaaa gagccgtaca aaattttatg gaatgggcaa aaaatattga
    13141 tgatacaatt gctaaaaggg aattttatcc agaagttgat aagaaagatt tatttgtact
    13201 aggttatatt gctgagaaat ctggacatag cagagggagt actgttgatt taaccatcat
    13261 tgaactttct aataaaaaag aaatagatat gggttctgca tttgactttt ttggaataca
    13321 atctcatcat gattatacct tattgagtga acaacaaatc tccaatagac taaaactaag
    13381 aacgataatg gaaaaatatg gttttaaaac tattgaagaa gaatggtggc attatacttt
    13441 gaaaaatgaa ccctttcctg aatcctattt tgatttccca attcgctaaa tgtcttatcc
    13501 tgaaataggt ttatacaata ataggtaaaa tgtgggtata aatgaaactt gtaaagtaat
    13561 tttagtttga tactgactaa atacattttt tataaaacca aacgaatagg tttcgctttt
    13621 tttatttaaa tttgatagta ataaaacttt cattatgata cttttaagtg caattacaat
    13681 tattatagcc atcttaatag tgctatttct aatctttgtc tataatcaac ttacaaaatc
    13741 tagaattttg ttaaaagaag cttggagtgg aatcggaata ttcttacaac aacgaaacga
    13801 tgtaatacca agtttagtag aaattgtaaa aggttatgta gaacatgaaa ataaaacatt
    13861 ggttgaggtt actaaatggc gtaataaaag tgctatggca acttcgcctg ccgaacaaag
    13921 tgaagcttca gcaggtttaa ataaagcact tttagacttc tatcaagtga gtgagcaata
    13981 tcctgaactt aaagcaaatg aaaactttat gcatttgcaa aatgaactaa ctgaaataga
    14041 agttaaatta aataatagca gaaggtatta taatggaaca gttagagaat ataaccaaca
    14101 aagagctgtc tttcctcaaa atattatagc atctttattt aattttttac ctgaagagtt
    14161 ttttgaagag gaagaaagtg caaaaattgc acctaaaatt aatttttagt gtaataaata
    14221 ttatatgaaa cgctgtttct ttataagtat tcaaatccta attctcatta gtaatttata
    14281 tgcaaaaaat atttcttttt cctctgaaac ggacgcactc ttcaatgatt ctagttattc
    14341 ttctttaata aaaaaagcaa cttttgatgt aataaatgta agagatattg atttgtattt
    14401 taatttgaat tatgcagtgc ttgaaaatga aattattgca atttcaaaaa aacagattgt
    14461 agaagactta tctaaagttt caaatagtaa aagacaacta aaaacttcaa aagcatatag
    14521 aatttataga caaacaattt atgatgccgt tcaaaaagta ttacttaaaa atgatacttt
    14581 actacagaaa ttactagaaa aaaacttgga tgatcaaata ctagactttc aaagtgaaat
    14641 tcatttaagt aaggatggaa aaattagagt aattgaaaaa attaaggttg caaattttaa
    14701 tcatggaaat aataatttaa ttcaaagagg aataacacgt gattttccaa tacaatattg
    14761 caataaattg ggttttctat attcagttcc attccatgta aattcaatta cacgaaatgg
    14821 agagcctgag aatttctttc ttgaaaatct aacaaatggg gtaagaattt attgtggtca
    14881 atcagatatt attttacctg aaggtattta tgaatatatt attgattata ccactgcaag
    14941 acaattaaaa tttcataata atcgagatga attttattgg aatataactg gcaacgggtg
    15001 gttgtttaaa ataaataaag taaaaggcaa tgtattctca cctcagacaa gcacgccatt
    15061 agatatgagt gtttatactg gaaaacaagg cagaaaagag agtaatgcca aaatttatct
    15121 tgctgattct actaatgttg aagtagtatc gactcaaagt ttattagaga atgaaggact
    15181 tacaatttct gtttcttatc caaaagaaac ttttatcgaa cctaattttc taattcaagc
    15241 cattcaatta ctaaaagaca ataaatattt attttttata atattttttg tttttataca
    15301 ttttattttg ctgtatcttc actggaaagc tgttggaaaa gacccaaaat caggcgtgat
    15361 tatcccctta tttgaaccac caaatgagct ttctccagct tctgttggtt atataaataa
    15421 acaatctttt aatgatactt tattttctgc tgcattaatt gacttagctg ttaagcataa
    15481 aattaatatt gatgttgaat ctaaaggaat tgtatttaaa tcaaatgttt actcttttag
    15541 aaccaataat attgaccaag atgattattg caaaaagaat tatggttttg aggaaacaga
    15601 tctaaatgga ataactatct caaaaggaac ttataatagc aaatttgctt ctatttactt
    15661 cctcttcaaa aaacatctag ataatcaaat tttaaaaata gacgaaaaag actcttcaat
    15721 tacaaaatat ttatcattaa actatgacaa aataggaatc ggattttttg cactttttgg
    15781 aatatttatc gctacaataa tttatctagg aacatataag ccacccgcca atcatattat
    15841 ttttttagtt tgtttgtttc tattagctgt tgtcgttcaa ataatattca cactaataat
    15901 taaagcttat accaatttag gtagaaaaac tgctgataaa atacaaggtt ttaagatata
    15961 cttatctaca acagaacaag atagatttga acgaatgaat ccgccagaaa tgtcgcttca
    16021 attatatgaa aaataccttc cttatgccat cgctttaaat gtagaaaatg aatggtctga
    16081 aaaatttaat gatattgtag aaaaggctat taataatggt gagcagcctg ttatgtatcg
    16141 ttcttaccaa tcaatgtctt cttcttcatt ttcaagtttt acatcaagtt tatcacaagg
    16201 cttatcaagt agcattagtt ctgcatctac tatcccttct tcatcaagta gtagtggaag
    16261 ttctggtggt ggaagttctg gtggcggcgg cggaggtggc ggaggtggag gttggtaaga
    16321 tgtaattcta ttttactgct ctatccttcc attttaccga aacaaaattg cccacaatac
    16381 caacataaac aatataagca atatgtaaaa tttcagaaat aatgaatcct tttaattgcc
    16441 ttttttcatt aaaaaatgaa ctggcttcat ttaataagat ataatctgct atcgttttta
    16501 aaacaaactg aaaactaaaa actatcaaga aaaataatac ggataaaaat tgtaaatagc
    16561 caacaatagt atgatttgga aaaatttgat aatacaaaat aataccaatt atcattgcaa
    16621 aattaacgac catactcaca caaaacaata aaaccagtcc taacatcatc gtcatacgct
    16681 tgtcttgata atccattgct ttacttgtcc aacgaaaacg ttgttgtaaa aatgatttta
    16741 aattatgaat aatttcagtt tgagtggtag cttcaatatt tttattaaaa aacacttttt
    16801 tatcatctat ttgggcaatt ttatacacca ataacatatc atcgccactt gctttttgat
    16861 tgataccttc atatccatta acttgtttaa aagcacttct tttataagct aaattggcac
    16921 cattccccat attaaaaata ccacttttga tgcaagctgc ggttattaat tgcataccac
    16981 aaaaatctaa aatctgaaat ctcgaaaaaa aggtaaaatg tgttgaagaa tgtaacaata
    17041 ctggagacgc tattaagttg gcattagtag attcataaaa ttgtacaatt tttttaagcc
    17101 aattttttgg tacaatgcaa tctgcatctg ttgtgataat tagttcttgg ttggtttgct
    17161 caacacctat cgaaattgct tttttcttat aggcattcaa atgttctaca tcaataaaat
    17221 ttttaagttc taaaacttta taagcaaaag tattctgttg taatttctgt tgaacaatag
    17281 ccaatgtttc atcttcagaa caatcatcta caacaataat ttctattaat tcttttggga
    17341 aatcttgttg taatatagca tctaataaat tagaaatatt ttgagcttca tttcttgctg
    17401 gaataatgat agcaacgctt gtatttactg aattttctgt atttgtaatg gtttcagttt
    17461 gcctccactg gtaataaaac aaaagttgta gcagaaaata ggctattgct aaacaaatag
    17521 aaattatcag ccataaagta aacatatttt tgcgtttata ccgattggaa ttgcttcaaa
    17581 aaacgcatat cgttttctga aaacaatctt aaatcattaa tttgatattt taacatcgtt
    17641 atacgttcaa tgcccattcc aaatgcaaac cctgtgtact tgttagaatc aattccgcag
    17701 ttttccagca ccgcaggatc aaccattcca caacctaaaa tttctaccca acctgtgtgt
    17761 ttacaaacat tacaaccttt gctattacaa ataaagcaag aaacatctac ttctgcagat
    17821 ggttctgtga atggaaaata ggaaggacgt aatctgattt tagtctcttt gccaaacatt
    17881 tcttttacaa agaaatctaa agtttgtagt aaatctgcaa atgaaacatt ttcatcaaca
    17941 tacaaacctt ccacttgatg aaagaagcaa tgagctcgag cagaaattgt ttcgtttctg
    18001 aaaacacgac ctggtgcaat aattctaatc ggtggttttt gttgctccat caatcgagtt
    18061 tggactgaag aggtatgtgt tcgcagtaac atcgcaggat taacttgcaa ataaaaagta
    18121 tcttgcatat cacgtgccgt atggtcttct ggcatattta atgctgtaaa attatgccaa
    18181 tcatcttcca tttctctatc taatgcaata gaaaatccaa ttttattaaa aatagaaatg
    18241 atttgacttt ttacaatatt cacaggatgc ctgctaccaa taggcaaatg ttcgcctggc
    18301 aaagttaaat ctatatttga agccgtttgc tgggtggtgg agaattgaac ttttgctgtt
    18361 gcaaactttt cttctgcaac caacttcact tgattcacta attgtccaaa ttcctttttc
    18421 tgttcattag gcacagcttt aatttgggca aatatatctt ttagaacatt ttttgtacct
    18481 aaaaatttta tcctgaattg ctctagttct tgtgctgaag caatcggaaa tgcttttaca
    18541 tcttctaaaa gttgttgtgt ttgctcaaat aaattcatac ttcaaaaata aaaaaagtgg
    18601 cttaataaaa ccacttttaa tataatataa tttagttaat ttcaatttag aaagaaatgt
    18661 cggcaacgct tatctttctt gtaatgacag gttttgctgt tgaatttcta attgtaacag
    18721 tatattcaat tggtgttttg ttccaatcaa tatcaatttg tccaaagttt aaaccagcat
    18781 acggagctcc acttcctttt attctgtttg tacttgcata cggcgaactt gtataatgtg
    18841 ttaatccact agaagtaaaa tcaaaaattg gataacaatc aggtactttc aaaacattct
    18901 catcaccaaa atgcacatct ccacttaaaa atgcaagacc attcactcga gttgatttaa
    18961 ttaattcaac cattcttctt ttctcatttg gcattacact ccaagcttct cctccattgt
    19021 aagatgctga aaattgagtg ccagaaacta caaatcttaa tttagcaggt cttttaaatt
    19081 gtgcttctaa ccaagcccat tgctcatcag acatcattct tgcgtctgga gttacaattg
    19141 aatcataacc agataatgca gaaagtggtc caggtgctgc ataaggtgtt ctgttccatc
    19201 tattatctaa caaaataatt tgaactctgt gtgcttcatc tccatattca taatttgcat
    19261 aaatttcacc actcgggtta aatcttcttg gatcatctgg tgcaactttg aaattatcaa
    19321 acaacaaagt ttttgcataa tactttgcag tattatcaat cacgccatca ttttgaccaa
    19381 aatcgtggtc atcccaaacg gctaatgttt caactttatc ataaaatctt tttacttctg
    19441 gctttgcaaa catgttttca taagccattc tcatatagtc ataagttcct ggtaaaatag
    19501 caaagaaatc accataaata ttatcgccca ttgctaaata taagtcagga tattgagcac
    19561 ccatataatt gtaaattcca acaatatcgt tgtgctggct tgaacacgaa ccaaaaagga
    19621 ttctattcaa ggttctacct ctatcatttt ttgcattatc atctctttta caagaaaaaa
    19681 gcacaacaaa actcattaat gataataata atattaactt attttttctc atgggtcgct
    19741 ttgattaatt aattctgtaa atttacgaaa actaaataaa ttaccataac atttttgaca
    19801 gaatatttat gataactctc atagattttt aaataaagac aggattatgc aaagttttga
    19861 acaaatgata gcaaaaattt ctaccaattt ggatagaaaa ctgcctggta cttcagcaca
    19921 caaaaaactc caaccttatt atcctagtgc gaaaaattta agtattccga caaatccatt
    19981 tgccaaaaaa agtgcagttc ttgttttgtt atttccaaaa aacaatcaaa ttaatgtggt
    20041 ttttatcgag cgaccagcga atacaggtgt tcacagtaaa cagattagtt ttcctggcgg
    20101 aggtagagaa aaaaacgata aaaattatac cgctactgct ttaagagaaa cgtatgaaga
    20161 aattgggatt gaggcctcaa aaattcaagt tctaggaaag ttatctaaac tctatgtggt
    20221 tgctagtaat tatttaattc agccgattgt tggttttatt aatgaagtac caacttatcg
    20281 tatcagtaaa aatgaagtgg acaatattat tgaaatgcct ttggttgatt taattcaagc
    20341 tgaaataatg gagaaaccta tacattctgc catgggcata actttacaag caccttatta
    20401 tgatttagaa ggcagaacac tttggggtgc aacagctatg attacaagtg aattagttga
    20461 tttatctact gatattctta aaaatatcga gttatagcat tttacgaaga caatactttt
    20521 gtataaacat ttgcatattt ttcaatccca tttgctaatg aaaaatagtc aattgctcca
    20581 ttgcgaattt tatctaaatc aaaaatttga tgccaatgct cctgatgaat ttgctctata
    20641 attgcatcat atttaaaatc tcggacaatg aatcctgagt ggtaacgctc taccacaaaa
    20701 tctgtatcgc ctacgcctgc attacaaaca ataggaattt gtaatgccat taactctcct
    20761 tgctttgttg gcgatgaggc ttttttagaa taagatggca aaataaaaaa aatagaaata
    20821 gcactcaaac tcaataacgc tgggacttct gttctattac cgccttgtaa aatgacacta
    20881 tcctcaacat tatatttctt agcaacttct aaaatatgtt gatgttcatc tttattaata
    20941 aacaaaaact tagcatttgt aattttggat tttaattgag caaagaatgc catcatttca
    21001 tctaacatgt accacgtacc aacagagcct aaataggtaa gaacaaaatc actgtcttga
    21061 atttgtaatt tttgtttcca attttcaact tgttgtatat caactttttc tttactaaat
    21121 aagttggtat caacacaaca tggaataact tctatacgtg gtttattttt tgtgaaattt
    21181 tcccaactta agatttcgtt ttttgcatta tgtgttaaac ttatagtata atcagcgttt
    21241 tgtaaaaatt caatttcttt tttcttaaaa aattgataga tttttctgta caatggatgt
    21301 tcaatattcc aaatttttcc gtccactcgc tcatcagccc agaagcctcg catatcaaaa
    21361 ataaacttag tgccaaattg cttttttgcc cataagccaa tcaatgctgt gatgtaactt
    21421 ctgcaatgaa taatttggat attatttgct ttgatttgtt tttttacaag tttttttaac
    21481 caataaacat cataaatcgt agaaaataca ggtggttttt tagtatattt taaaggttgc
    21541 caaataattt gatttgtatc tgcaatttgt tgaatcgtag ctttgttttt ctcaaaaact
    21601 tcttcttttt caaaacttat aatcgtgata tgataattat gtttagacaa tccaactaaa
    21661 tacggcaaaa cttgcgattg tcctaagctg tctgtcatgc catcatacga caagaaaagt
    21721 gtatttattt ttccttgttg agccatttta cgcttcttct tttaattcta ataacactac
    21781 attttcaata tgataggtat gtggaaacat atccactgcc tgactttcta ataaattata
    21841 ttgagctgac aacaattgca catctcttgc ctgtgttgca ggattacaag atacataaac
    21901 aatacgtttg ggctttgcta tcaataaggt ttcaacgact tgattgtgca atcccgcacg
    21961 tggtgggtct aggataacaa tatctggcat ttgattttct tgtataaatt cctcatctaa
    22021 caatttttct actgttccag aataaaattt tgcattcgta atttgattca ttcgagcgtt
    22081 ctcacgagca tcaataattg catcatctat ttgttcgata cccactactt gcttacaata
    22141 ttttgccaca tacaaggcaa tgcttcctgt gccacaatac aaatcataca caatatcatt
    22201 aggctcaatt ttagcaaatt ttacggtaac atcatacaat attttggctt gaaaactatt
    22261 agtttgaaaa aaagattttg tactaatttt atattttatg ccatctaaaa cttctataat
    22321 ataaggtctt cctgaaaaat ttataacttc ttggtcataa atactatcat tctttttcgt
    22381 attaattaca taattcaaag aaatgatttt ggggaaattt gtttgtatat cctgcatcaa
    22441 taaatcaatt tgtttttgat tggcttgagc cacacaaact gtaatcatcc attcgcctaa
    22501 ggtggtattt ctaataatta aatttctaac aaaaccttct tgcaatttca aattataaaa
    22561 ctcaaaatta ttatctacac aaaattgata aatatgattg cgaattgtat tggcaaaatc
    22621 atcttgtaaa taacacttgt ctatttgtag aattgaagca aaactacctg caacgtgaaa
    22681 acctaaagct cttttttcaa aaacctcatc actttgaatt tgttcatctg tcaaccatgc
    22741 acgattggaa aatgtatatt ccattttatt ccgatactca aatatttgtt gagcggccaa
    22801 tatcggcaaa ggattttcga ttttaatttt tgcaattttc caaaaagtat ccaacacaat
    22861 ttgctgctta aattccgctt gagttgtata gttgacactc tgccaataac aacctccaca
    22921 agttccaaaa tgcgaacaaa ccgcatcaat tctttctttg gaaggttcaa gtattttttg
    22981 aattttagct aaaccataat cttttttctt ttttatgatt tttgcctcca caacatcgcc
    23041 aggaatcgct ttttctacaa aataaatgcc ttgttcagtt ttggctattc catttccttc
    23101 tgcggcaatc ttatcaattt ttaaaacttc tattttttga acctttctca ttggttgtta
    23161 aaaataatta aatgccttta caatagtgca tatttcttta tatattcatc tttaaattct
    23221 atatttttgt ttatctcaat attttatgca gtatgaaaaa gtctctactt gtcttttttt
    23281 taggttttac tttattttct tccaaagctc aagatgatgt tttatttacg attggtaatg
    23341 agcctgtaaa gctgagtgag tttgaatata tctacaataa gaataatttc tcaggagcaa
    23401 acaaatacag caaaaaatca gttgatgatt atttggactt atatatcaat tttagactga
    23461 agctaaaaga agccacagaa ttgggtttat tcaacaacaa taaatttaaa gaagaatttg
    23521 ctacttatga aaatcaactt atcaataatt atgtagaaaa agaagtattg gataaagttt
    23581 tattgcaaga atatgaacgc tctaaaactg atgtttcatt gagtcatatt tttgtgcctt
    23641 tctacaatga ttcgactaaa actattgcgg ataaaaaaat caaagctatc tacgaaaaaa
    23701 ttaaaagtgg aaaaagctcc tttgaaattg ccgcaaaaga atcagaagat aaaaacacaa
    23761 atacaaaaga tggttattta ggttggttca atagttatca aataatttta ccagaattgg
    23821 aaaatgttgc ctatacctta aaaccaaaag aaatttcagc tccattccaa tccaaatatg
    23881 gctatcatat tctaaaatta aatgatacaa gaccagcacg tcctatgttg agagtggcga
    23941 ttattaagaa aaatattcta aatcaaaatg accctgcaag tgttcaagca ctgagagata
    24001 cgatgcaaaa aatatatcac ttatttaaaa gtggcacacc tttcaatgat ttggttcaaa
    24061 attacaccga tgatgaaaat tcaaaacaat ttggtggaaa gattgattgg tttggtattc
    24121 attattatac gcctgttttt gaagaagctg catatagtat aaagaaaatt ggtgatattt
    24181 ctgaacctgt tgaaaccaaa ctagcttttt atctgattca aaaaattgat gaaacaaaac
    24241 caaaaagttt tgaagaagaa aaaacaaatt tgagaagtaa attacttgtt tcagatattt
    24301 atgaacaagc tatgcaaagc tttttaaata ctcaaaaatc aaaatataat tttattgcct
    24361 ttaataataa tatctataaa tttaaacaac accttaataa tttaatagga aatacagctt
    24421 tcaaatataa agacacaatt ccaaataata ttttatacaa aattggcaat caagattttg
    24481 accaaaataa aattggaaaa gctatcgaaa aaatctacta cagttatgca ccaagaaaca
    24541 ctgctgattc aagaattgat gtattgtatg atgaaataga aaaagaaaat attttagctc
    24601 aagtaaaagc agatatcaga agtaataatg aggaatacaa acaactcatc aatgaatata
    24661 aaaatggaat tatgatattc gatttatctg aacaaaagat ttggaataag gcatcttcag
    24721 atactgctgg attaaaacaa ttctataaca cacacgctgc tgatttttta caaaaagcaa
    24781 cttacaaaga aagaaacatt accatttctc aatcgaaatc ggcaaaaaaa atatataaaa
    24841 ttattttaag caatccaaat ataagcaata acgttttgaa agataaactc tcactcttag
    24901 atgagaaaaa tacaacgatt gatgaaatta cacatactgt ttcaagcaac aatgctattg
    24961 atatgagtat taaaaaacct attaagaaag aggataaatt tgtcattaca caacaatatc
    25021 aatacacgcc gcaacaacca aaaccttacg acgaaataaa aggatatgta attgctgctt
    25081 accaagaaca attagaagaa aattggataa atgatttaaa agctaaatat cctgtaaaaa
    25141 ttaacgaacc tattttgaat aaaataatta aacattaaaa cattcatttt aagtaaaaaa
    25201 cacaaacaaa accttacttt tgtatttatg ataagaatat tggtgcttac ttttcttttt
    25261 ggtttgattt caaataactt gaatgcccaa aatggaattg tattggataa aattgatgca
    25321 atagttgcag ataaaattat tttacaatct gacattgaaa atcagatgga aataatgaat
    25381 ttgagagatg agaacgctgc tgaaaataaa tgtgatttaa tgtatcaaat gattttcaac
    25441 aaaatcttaa ctgttcaggc agaaaaagac agtttgccca tagctgaaag cgatgttgag
    25501 gctgaattgg atagaaaaat caattatttt atttcgatgg caggttctgt tgaagcattt
    25561 gaagcttatt acaataaaaa tgttcaacaa attaaagacg attttagaga tgatatacgt
    25621 gagcaaatgt atgctgatga aatgagaaga aaaatcgtgg gggaattaaa agttacacca
    25681 tcagaagtaa aaacattttt tgaaaacatt gctcctgaag atattccata ttttaatgca
    25741 gaagtagaat tgggacaaat cgtggtcaaa ccaaaaataa gtgatgaaca aagacaaatt
    25801 gctctaaata aaatcaatga tataaaaaag cgattaacag aaggagaatc ttttgattta
    25861 ttggctggtc tatattctga agacccaggt tctgcacaag aaggcggaga tttaggttgg
    25921 gcaaatcgtg gtacttttgt gaaagaattt gaagctgtcg ctttcaaatt aaagcctgaa
    25981 gaaatttcag atgttgttga aaccacattt ggttttcata tcattcagtt aatagaaaga
    26041 agaggcgatc aaattaattt acgtcatatc ttaattcgtc cgaaatcaac ttcggctggt
    26101 gcagaattag cttacaaaag aatcgacagt attagaactg caattttaga taacaaatta
    26161 gctttctctg aagcggtaaa gcatttttcc gaagatgaac aatctaagaa tgtaggtgga
    26221 tatttaataa atcaagaaac aggaagtaca accaacgaag taaaagattt agacccagaa
    26281 atgtataaag caatagagaa tttaaaaatt ggcgatatta cagaaccaca aatgtttcaa
    26341 tctggcgatg gttctccagc atatagaatc ctaaaattat tttcaaaaac agaaccacat
    26401 gttgccaatt tgcaacaaga ttacgataga attcaatcag cagctttaaa tcaaaaagaa
    26461 caaggtgttg ttgaaaagtg gttacaaaaa aatcttggaa aaacatattt gatgttagat
    26521 ccaaagtata aatcttgtga aaatttaaaa gattgggtaa aagaataaag ttcaattatg
    26581 caaaaattta cttctgatac cgaaggcatc caactattag caaaagacta tcaaagtcta
    26641 aaaaatgaaa ttcataaagt cattgtcggt caagataaag tcattcaatt tgtattatta
    26701 tctatttttt cagacagtca tagtttgttg attggcgtac caggattagc caaaacatta
    26761 ttagtcaaaa ccgttgcaga tactttagat ttagatttta aacgcattca atttacaccc
    26821 gacttaatgc cttcagatat tattggagct gaagtcatta atgaaaatag acaatttcaa
    26881 ttcaataaag gcccgttatt tgcaaatatt gtattggcag atgaaatcaa tagaacaccg
    26941 cccaaaacac aatctgcatt attggaagcg atgcaagaaa gacaaattac gaccgcagga
    27001 catcaatata atttagattt gcctttcttt gttttggcta ctcaaaatcc aatagaacaa
    27061 gagggaactt atccattgcc tgaggcacaa ttggatagat ttatgtttag tatccaattg
    27121 gattatccaa gttatgaaga agaagttgct gttgtaaaaa atacaactac agaccaaaaa
    27181 attgttacca aaaaagtaat tactaaagca gatattattg catatcaaca cttaataaga
    27241 agaatgccga ttgcggataa tgttttggaa tatgctgtaa aacttacttc atcaactcga
    27301 ccaaatactt caaatactcc aaaaattatt caagattttg tggcttgggg agctggccca
    27361 agagcatcac aagcattagt tttgggagcg aaaacccatg ccgctatcaa tggaaagtat
    27421 tctccagata ttgaagatgt tcaaaatgtt gctttaccaa ttttacaaca tcgtgtaatt
    27481 agaaatttca gagctgaagc tgaaaatatc agtattgaaa aaatcataaa agatttattg
    27541 taagcaagtt ttctcctttt tattttttta attctacact tttatactta ttttgtataa
    27601 atgaataaga attccgattt aaaaattttt aaagcctctg ctggttctgg aaaaacatat
    27661 acactggcta aagaatttgt aaaaattctt attgtacaac cttatcatta taaaaatata
    27721 ttggcaatta cttttaccaa aaaggcaact gccgaaatga aaagtagaat tttagccttt
    27781 ttaaaagaaa ttaaagaaga taaaaattca aaactaacac tagaaattat tgaggaaata
    27841 aaagaagaaa aagcggttga tgtcagtcaa aacattcagg aacaagccac gaaagccatc
    27901 aatcttattt tacatgatta tggacatttt caagtaacaa ccatcgatag ttttttccaa
    27961 ttaatcattc gttcttttgc taaagaattg aaactaccaa ttggtatgca aatagaaatg
    28021 gatacagata gtgttttaca caatagcgtt attcaattat tagaagaatc tgaaacagaa
    28081 aatgaaattg cccaatggtt ggaagaatat gctctaaata atattgataa tgataaaggt
    28141 tggaaaatag aaaaaaatat ttttacccta agtcaggaat tgctgaaaga agaatttcta
    28201 ttgctgaatg aaaattttga acaaataaga caaccagatt tttctaaata caaagctatt
    28261 gctgatgaac aacaaaatat tattaatata tataagaaaa atatccaaaa caaagcaaaa
    28321 ctggctgaag aaattataaa agaatcagga attaatgcaa aattatgctc ccgaaactgc
    28381 ctaaatagtt ttattaaaaa ttcaaaagaa ttaagttttg atagttataa aaccattaga
    28441 aatgttgttt ttgaaaatgc caatttagtt tcaaatgata atgccaaaaa ttatgttgaa
    28501 gcaacagcaa aaattatgtc tgtttgggat aatcaactaa aggttattat tgaagatatt
    28561 attaatcatc atgaaaagca tattagcaat tatatcaccg ctagaagtat tcaaaaaaat
    28621 atctattcga tggcactctt agatgcattg tctatcaaaa tcagtgaata ccgaagtcag
    28681 gaaaatgtga ttttaattac agatgcatca ttttttattt ccaaaattgc tgatacagaa
    28741 gctacgccat ttatctttga aaaaacgagc agtttcatca attatatatt gatagatgaa
    28801 tttcaagata cctccacttt gcaatggaaa agctttatgc caattgtcat tgaaatttta
    28861 tccaaaggat atggtttggc tttaattgtt ggcgacccaa aacaaagtgt atatcggtgg
    28921 cgtggtggaa aaatggaatt gattattaaa aaagttgatg atgacttaaa taattttaca
    28981 agcctaaaaa aggaaaaaac actcagaaat aactttagaa gtttgacaaa tattattgac
    29041 tttaataatg ttttttttag gacaacaata gaaacctaca aagattttga attgctttca
    29101 aaagcatatc acgagcaaga acaaacgcaa acagaagaag taaaagctaa aaaaaatggt
    29161 ggattgattc gttgtgattg gattgagtct tcaaaaaata aaaaagattc aacgttggaa
    29221 gatgatgagg aagataagga aactgaaaat aaaattttaa ttgttttaac agaaagactt
    29281 tatgaattaa ataaaaaaac caattggaaa gatgtggcta ttcttgttcg cactaataaa
    29341 gaaggtatta aaattgctaa ctacttccaa caattagaaa aaccaattcc atttgtttct
    29401 ggtgaatctt tgctaataaa aaacaaccta aatatccaat tgattattgc tacattagaa
    29461 tactttatta gtgatgaggt tttttataaa tataaattag agaattttta ttgtaattat
    29521 attggtataa aaaattacga tgaaatatta tatcataaac aaagttttga atttgaaaag
    29581 cgtttcaaag aatttaaaat aagcaagcat ataaaaaata acaagctaat tgatattgtg
    29641 catacactgc tccatattta tgaaatggat aaagaaagca atgcctatat tgtacgtttg
    29701 ctagatgata tttataaatt ttctcaaaat aaaggccata atatcattag ttatttatac
    29761 tattgggaag agaaaaaaga caagatttct gtgttgcctt ctgaaacaac cgatgctgta
    29821 aaaatcatta cgattcacaa agctaaagga ttagaatatc cgattgtaat actacctttt
    29881 gcgaattgga gtttaatgcc taaggcaaat actattttct gggcaaaaag tgaagtattt
    29941 aaaacagaag gtcaagtaat gccattgtct tatgtcaaaa accttgaaca atccgaattt
    30001 aaaactgcct atttaaaaga aaaagaacaa agtattattg acaatatcaa tttgttatat
    30061 gttgcattca cacgagcaga gaaagagttg catatttttt gcaaacaaaa aaaagctaat
    30121 cctgatgaag caaaaagcat aaatgatatt ataaccaaca ccttaaacaa cgcattttct
    30181 gaaaattttg taacagacaa aagctttata cttgggactt ctgcaaattt tgaagtaaaa
    30241 gagaaagaaa ataacgatgc aaacaactta aaattagctt cgattaatac caatcaaaat
    30301 ttccaacttt tatcatcgtc ttattccgat gaaaatacta aaaaaggaga tattatacat
    30361 catttggtca ataatattca tagtttgaaa atgaataaac ttgatttata ttccattcaa
    30421 cgacaatatt tattagatga tgaagctatt gtttattata aaaataaatt gattgaaatt
    30481 tttaagctat ttatagaaaa aggttttatt caagctgaaa atacctattt gtatgaaaag
    30541 aatttttatt ataaaaataa ggttttacgt gcagatttgg tgattataac acctgacgaa
    30601 aatattatca ttgattataa aacaggtgcg gaagaagata aacataaaac tcaaattaat
    30661 acttataaaa aagcgtatga gttaatgaat aaacaaaaga ctaaagcctt tttgctttat
    30721 accgaatcca tgacattaaa agaaatttag tatgcatttt ataaaaaaat atattgaaga
    30781 aagttttgaa attattgagt tgtctaatca atttataaac gccaaaattg ttgtacaaca
    30841 gggatttaat ttatttagtt tagtctataa aaatatagaa accatctatt ttgataaaac
    30901 attaattgaa aattttcagg atagaaaatt agctggtatt ccgtttatgc atccttgggc
    30961 aaatagattg gaccgtgata aaatttttaa tcgttcattt acgatgaatg atatattaaa
    31021 tgctgtttta tatcgagatg gaaataattt agcccttcat ggtttaattt taaaatcaga
    31081 taaatggcaa attatagata tgagtgcaac agatttaaaa gcttcttgta ccgcaaaatt
    31141 agcctttaat gaaacacatt tattagactt atttcctttt aagcataata tttttattac
    31201 cctagaatta gaaagtgaaa aattatttta taaggttgaa atagaaaact taagttcaaa
    31261 tgaaatgccg atatgctttg gatttcatcc ctatttcaat ataaaacatt ctgaaaataa
    31321 tattttaatt gttccagatg cagaagtggc attggtcgat gaaaaattaa tacccacaaa
    31381 taagtatgat gaaaaagaag cccttttttc atttaaagaa aatcaaatac ctatcaaaaa
    31441 ccaaaatatt gatcacgcat ttttgcacca aaaccaaaac gaaaactatc aattaaaaac
    31501 agataattat ttggtgacgt ttaatttaga cgaaaattat actgttgtac aaatttatca
    31561 tcctaataac gaacaaaaac cttatatctg tatagaacct atgacagctt tggcaaatgc
    31621 tttgaatact ggaaattata acactattgc acccaactca aaatttaatg ctgtttttag
    31681 tatagaatat tgttctacaa tagaacatta aaggaaagtt tattaaataa aaatgccctt
    31741 gaactaacga gttacaaggg cggaggtttt ggcacctgtg atcccgctgg gattcgaacc
    31801 caggacccat acattaaaag tgtattgctc taccagctga gctacggaat cctgcttctt
    31861 gaatgtttat ccataatagc taaacattaa aagcgagtgc aaaggtataa aattatttct
    31921 atttgaacaa ataaaaataa aatctaatgt ttatttaaag taaggaaagt ttggattgta
    31981 cgataaacat tgataaactg atttatttat cattgaatta ctttaatatt gatttgattt
    32041 ttggctgaat aagttgttga acatattggt ctgctccaaa ttgggtttta atattaattg
    32101 gtaaggtaca aagtcaggtt cttgtactga tgcaaatgga aaaattgata acagaatcta
    32161 tgttatgaat gaaactcttc aacctgtatt acatcgattt ctaggaggtg aagttacatc
    32221 tacctattaa atatatttag tttatggatt tgaaaataca gttactacaa ctattttgca
    32281 acaattgact ttgaaaaatt tattgaagaa attgaacaga tattaaattt tgttttttaa
    32341 aaaaaaagta attactttgt aattacaaat aggatttttt atggaagtac aagtaatcaa
    32401 tattggaaac tcaaaaggca ttagattgtc aaaatcaatt ttagagcagt ataatatcag
    32461 tgatacgctt gatttaattt tagagaaagg cagaattatt ttaaaaccaa aatctgttcc
    32521 tagaaaaggt tgggaaaaat cttttaagct tatgcacgca aacggagatg accaattact
    32581 gattgacgac atttttgaag atgaaacttt tggagaatgg aaataaagca atacgaaata
    32641 gtgttggtaa atcttgaccc aaccatagga agcgagataa aaaaaactcg tccttgtgct
    32701 gttatttcgc caaatgaaat gaataaatat cttcaaacga ttgttgttgc accactcaca
    32761 agcagttcaa aaccttatcc aacaagagtt gaaattaaac aagcaaaaac aaaaggttgg
    32821 atagttttag accaaattag aacggtggac agaaaaagaa ttttcaagaa attaggcacc
    32881 cttacaaaaa tagaaatcga agccgtgaaa aacgtaataa tggaaactta tgttgaataa
    32941 taatgccata taacaaaagt gcttatgaaa aacagactaa aaatcaaaat ttcaactttt
    33001 ttgcaaaatg caactgaagt gcttcaattt cccatccttc ataaatacca gaaccgatag
    33061 aatatgctag agaaacaaat aaaaatgaaa tctaatgttt atttaaagta aggaaagttt
    33121 ggattgtacg ataaacattg ataaactgat ttatttatca ttgaattact ttaatattga
    33181 tttgattttt ggctgaataa gttgttgaac atattggtct gctccaaatt gggtcaagtg
    33241 atta
//
LOCUS       DAOQCW010000013        30489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_552_length_30489_cov_6.060262, whole genome shotgun sequence.
ACCESSION   DAOQCW010000013 DAOQCW010000000
VERSION     DAOQCW010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 30489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30489
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_552_length_30489_cov_6.060262"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..5700
                     /locus_tag="PLS16_02275"
     CDS             <1..5700
                     /locus_tag="PLS16_02275"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNJ59932.1"
                     /translation="TVTADNGSCTSTSATFTIAGQLTTPATPTVSVTAPTCTDAGFAQ
                     VTNYDGTLNYTLSSNNISIDGSGKITGAAGNYTVTVGNGSCSATSASFSIASKLVVPS
                     VPTVNVIAATCSTAGSAQVSNYNSSLVYTVSPNTANIDGSGKITGAAGPYTVTADNGS
                     CTSTSATFTIDALISSAPTPVLSADNTTICAGNSALLSSDVTTVKWYRDAAKTNVLGT
                     GTPISTGNLSATTTFYAENTSGACIGALASITINVVAAPSAPTATKLPNKSSVCIGTT
                     LTIQNPQYGTNSGNSCPFEYQSSTDAGQTWSAVSSTVPSLIADGTENIIRIRVSACNG
                     CTASNWTDYSWTVISDTARPAITGNLSVCTGTSTTLVANAPIGSTVRWYSDNGLTTLV
                     NTGTSFSPIITSNTSFFVTQTQDLCTSAATQVDVVALPAPAAPTLTLGHSPICGGQNT
                     DLYSSSSTVVWYLDAAKSNRIGVGSTINTGTIYTTMTYYAADTSGLLCPSPLSSITVD
                     VIPAPDLGNIVDVSICSGTSALLTGGSSATEWYSDDAMTNNINMGATYQTPILTATTN
                     YYLRVPSSCGVTLDTVTVTVKPIPAAPTANDVEVCSGLSASIALANVSPGATVQWYNA
                     PSLDNMIHVGSVLITPILTSDAVYYVTQTVDGCTSPSSSFNVYVKPSSNAPTLTANPN
                     VICFGDASALSSSSTATIWYSDAGQTVIGNGQTISVSPLLTTTYYAQDTAVGCSALAS
                     VTVKVDSILPAPNIQDVTVCQGADAVLTGGNSGTAWYAADQTTLIGTGATIIVTNVQS
                     ASLYYARNEGSSCVSAFDDVTIHITTTPPPTAADAEVCSGQSASLSATGQNIVWYSDA
                     GLTNAVQSGSVLITPALTSDVTYYVTNESNGCESEATAVQVTVTTPAPAPVITANPTS
                     VCSGGTTELSSSAGANTVWYSDAGMTVVGSGSPFTSAPLLATTTFYARDNSVPNCPGE
                     LASVQVLISASETAPNVSNTSVCEGSSATLTGGTANTHWYSDASLTTEVNVGTNYTTP
                     VLSTATTYYLRNENGLCVSPVTEVTVSVNPIPAAPTADVVEPICGSGSASLSAQGDAN
                     AIITWYADGAMTNALHIGSSYITPILTANTSYYVTQELNGCESAVTEVEVEINELPTI
                     DPVDPQQVCSGSTAIFEADNPNGYDIEWYSDPAGLDLWQIGDEFETPVLTQYTAWYYR
                     AVDPITGCEGEIQSTWADVVSNKDITITATSKPTCVDEDIVVNVASYDFSGLVGIFDY
                     DGNLVAVEDINGTTGGTQVILPGLPVAGVYSYYVLEAGDIFCATSSSTFTVEVVDRPV
                     IDNVVGDEICIGEVAQITATATPANAIITWYSDATLANAVGTGNTLTIPGLTVNTTYY
                     AVASNGLCASDPMDVEVIVNPIPATPELSSNSPVCEGSELTLSTTTAGNVTYIWSGPA
                     GFLSGDQNPVIASVTMANQGLYSLKVQDNVSGCTSLTASVQVNINTAPTAPTASNSGA
                     TCEGGSAILSATTIPGATYEWTGPNGFSGSQQVITLNQVTLAQAGTYSVRAFVNGCWS
                     SATTTDLVVNEKPIVSIPDVKPVCEGGNLEIIATTNSANPVYDWRGPNNYVGQNATVT
                     IQDVKESKHQGAYTLQVTDGTTGCKSDVVATWVVINEKPNNIMAINNSPKCEGGSVQL
                     NASNIIGATYTWTGPDGFASQDRSPIITDLNANKVGTYTLTVTLGDCLSETVTTTVEM
                     NDKPVANAGDDITTQTNVPVKLNGSGGIIYAWSPANMLSFSNIPNPTFIAGSVGVYEM
                     ELTVTNEFGCSDADTVVVNVEKATTNLDSVVIPDLITPNGDGINDVWRLDFLKDINMS
                     YEIAVYARGGSKIMISTNYNNDWTGTYKGKELPDGVYWYAIVLENGAVYKGSLTIKR"
     gene            5744..7285
                     /locus_tag="PLS16_02280"
     CDS             5744..7285
                     /locus_tag="PLS16_02280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNJ59933.1"
                     /translation="MKSIKYLITCLFLSLTFVVSKAQQTPVYNNYTINPFLYNPARAG
                     DNPDGGVINLGFRKQWDRIPYGPVTGNATWDSRIKESNMALGASLTHDRVGKHIMNTM
                     ASFTYNYRLPFNKEKNHGLNFGIQAGIISQKFNFTDAITTDGIFNDNAAVTNNSRATK
                     FDLSLGINYHWKGLNVGFSVPQLLNGKAKFRGNGMDNETSMFHYEREYLVLASYEARF
                     GGKDKKTWMITPSLMFRKYKSIDPQIDLNVMFGYKQLVWLGAGYRTGGGYGGFIKTNS
                     AGFHVTAGAGIKERVNLFYSFEMPMKDVRSNFGYTHEVTIQCNLAKKVDKSEYERNKK
                     KVEDDIAKLNQKTDEVGSKAIEAMDKGNDLADQVKNADNSIKEAFEKLNAQEDNIKEI
                     ASRLDNIVFKKFGSIYFENDKSELTAEAKASLDAFKSKLAGMKGNYFIYLAGNASQEG
                     ATDYNLALSAKRSDAVKKYLEGVGISQRILLLPYGENSWVIDKQDNEADKAKNRRVDI
                     YLAGE"
     gene            complement(7788..8846)
                     /locus_tag="PLS16_02285"
     CDS             complement(7788..8846)
                     /locus_tag="PLS16_02285"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNJ59934.1"
                     /translation="MKHFNSQQILDDLKNWQSMVKKYQVPNTKEAIIQMANSFSFYVL
                     LLGLQFYLYEKSFFWSALVALLNGFILSRIFIIQHDCGHSSFLKSKKANTIIGTICSI
                     CTLIPYKYWAKNHGFHHAHNGQLEVSDIGDVDCMTVEEYVKANWKTKIYYRVYRNPFY
                     LFTIGGFIYVVVYNRFAFSNLSYFKKVKNNVNISNAIFIITYAIAAYILGPERFLVVQ
                     FTNLFFFGIYALWFFYIQHQYEFIYKSGKENWNYVVAAIKGTTYYKLPRIFHWLSGNI
                     GYHHIHHLSSSIPNYNLRKCHQENPILAKHTNTITFWQSLKTIHSNLWDEEQKKMISF
                     KEYSRRKKLAKQMALKAQ"
     gene            complement(8976..9869)
                     /locus_tag="PLS16_02290"
     CDS             complement(8976..9869)
                     /locus_tag="PLS16_02290"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNJ59935.1"
                     /translation="MIKKTIGYITIIFIKIMSFLSNHFFGIISYLIYILIYKIIKYRV
                     TTVRINLMTAFSDKSSYELKQIEKQFYKYFAHLISQNLQSYSFKEHQVKKYIWLHHDA
                     KQLIWALEKNNQPIFIVTSHFGNWELSVFLAAMETRFKTYGIYQKLSNQTIDRFVKKN
                     RAKFGIELIETKLIKNIFKNLDNNNSMFCFVADQTPVAVNKATWINFFGVETPFYNGY
                     AHLAQKINAVILYASIEPINNTHFEIKLKEITKNSANESIENIVDGFAKNLEQDIKKQ
                     PAYWLWTHRRWKRADIIYYDK"
     gene            9997..11091
                     /locus_tag="PLS16_02295"
     CDS             9997..11091
                     /locus_tag="PLS16_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNJ59936.1"
                     /translation="MKIADIIACIEDFAPKSYQESYDNAGLIIGNAKNECTGVLICLD
                     SIESVIDEAIAKNMNLVVAHHPIIFSGIKQIVGKNYIERIIIKAIKNDIAIYAAHTNL
                     DNMQNGVNARIGEKLGIKNSKILQPKSKVLKKLYTYIPTTHTQNVAQKLFEAGAGNIG
                     NYSECSFTINGKGTFLPNENANPTLGKKLERHTQNEDKLEVLFPFHLEQKILNTLFQN
                     HPYEEVAYEIITIDNKNQTIGSGMIGELDKPMNETDFLYFAKQQLNTKCIRYTALQNK
                     PIQKVAWCGGAGNFLLGDAIAHQADVFISGDFTYHKFFDADNQIIIMDIGHYESEQFT
                     SEIFLDILTKKFRNFAIQISENNTNPINYI"
     gene            11093..11860
                     /locus_tag="PLS16_02300"
     CDS             11093..11860
                     /locus_tag="PLS16_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HNJ59937.1"
                     /translation="MAKKKELTGVTDKLEQLAGLQAIHSKIDQIKVLKGELPIEVEDL
                     ENEISGLEKRITKIGNELKETEDNITQRKQTIKDATNLIAKYDKKLTNVKNNREYDAL
                     NKEVELQKLEIQLAEKKIKDATFSLESSKTSLEEAEAKHAQKISNLNSKKKELSHIIE
                     ETEKEEKELSAQASELETHIEERLLTAYTRIRKNYKNGLAVVNVERNACGGCFGFVPA
                     QTQAEIRTKKRIISCEHCGRILYGVKDDVELSAEEIA"
     gene            11916..12965
                     /gene="lpxK"
                     /locus_tag="PLS16_02305"
     CDS             11916..12965
                     /gene="lpxK"
                     /locus_tag="PLS16_02305"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNJ59938.1"
                     /translation="MLLKPLKYIIAIIYEAIIRFRFRLYKHHFLGQTSFDIPIICVGN
                     LSVGGTGKTPMVDYLCTLLENQFAIGIISRGYKRKTKGFIRLKAKHTATEVGDEPLLL
                     KLKHPQIEIAVGEERIYAIPHLLSDAPQTQIILMDDGFQHLSVQAQLNILMTDYQQPF
                     WSDKILPLGTLREPKEGYKRADIIIVNKCPDNISKVDMQTCIHKIEALPNQKVFFAKI
                     KYRNLYALFYNIVPNADENPYILFTGIANAESIFQYLNPKFRIFHFQFDDHHAYSIKD
                     FEDMYAEIKTNNWITTEKDAVKLFPFKTWFESKQINVWVQPIQLEISSIDNASFDELI
                     HQYLDYYFQTNTETN"
     gene            13001..14872
                     /locus_tag="PLS16_02310"
     CDS             13001..14872
                     /locus_tag="PLS16_02310"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59939.1"
                     /translation="MLGFLMISFLFAFNANAQTRITTPNPILRYDTKYITLDNDELRK
                     LDSNNHNFHRYNVTEKQSVPYYNLGNYGTAYYPLMFQYNRNIGFSSGYNSFDLYKRNK
                     LDIQFFDTKTPYTDLDFIFGAKEEITGGADFSMNVNPQVNFGLYYRRNSFKGQAKLQE
                     SVYNNFSLNNWYRSKSNRYEIKSVFLFNNVKNQENGGWAVGNIYNDAIYKGKKNKQFA
                     DVNLQTATQKYTEKNVFFIQDIHFGKKEIVEVNDSTKQTKITPRYTLEHEFSYKKETY
                     LFKNYKQDSTFFQQHYLSTDSTHDATKTWNISNSLAFKNVADSTTKIPFLYKVGVQLD
                     LIKYKQFYNNKFITDIKLFAEIKNKNKQSKLQYFANAMIDIAPKYIGDFEVDLGASYF
                     INKEIAIELTNKIQHRSPSEKENFLVSNHFLWQNNFKKTFHNQTNLVFNWAKQKFYIN
                     FENHILKNYIYYDTATTLQQYNKNLIAFNVQIRKDFEFKNFYIGNTFWVQFFNNKDII
                     KLPTFAMLETFYYKGGWISGKLNAHLGVNISYNTNFNTPKYQPALAEFYIQNTERMKF
                     YPILDVFFNLYVSKTTIFMRLEHANQGMFKQKGIYTSPNYGYLDRTFRVGVNWQFYD"
     gene            14865..16070
                     /locus_tag="PLS16_02315"
     CDS             14865..16070
                     /locus_tag="PLS16_02315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ59940.1"
                     /translation="MIKQRKKILFLTPQLPYPVHSGGFIKSYRMLVHLAAQHDVDFAS
                     LLKGEDVQLLEEFKASIPNIMQFFTVPSNIERNAINILKANLQFKPISFYRNFSKQLD
                     KLLQTIFANYDIIIVDHVDMFQYIPKQYFHKTILHQHNAEYVMWKRAAEVESNFIKKI
                     VLFVQFYLLGKLEHTYCNQAKYILASPNDEVELKRIGVRNNNFFETYHLGDDSLLHQT
                     PLVFDKENKNLLYIGTLTWEANIDGLIWFLDNVWQTLSNENKGIYLTIVGKNPDKRLL
                     KFKQQFDNIIFPGFIENVEPYFQNAKLFIVPLRFGSGIKVKVISAMYRGIPCVTSDIG
                     AEGLMVENCKQIMLANDVKSYINAIQTLLNDEDKWNTISKESRILAQEKYSWEAVFKV
                     IDEVITKFP"
     gene            16194..16829
                     /locus_tag="PLS16_02320"
     CDS             16194..16829
                     /locus_tag="PLS16_02320"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNJ59941.1"
                     /translation="MALRLGDIAPNFTAETTEGNIDFHNWMGDKWAVFFSHPADFTPV
                     CTTELGYTSKLKSEFEKRNVKAIALSVDDLASHNGWIGDIEETQGVKQNFPIVADEKR
                     TVAELYDMIHPNETNKFTVRSVFIIDNNKKIRTIITYPPSTGRNFDEILRVIDSLQLT
                     DNYNVATPVNWKEGEDVVIAPNISDEDAKVKFPKGFKTLKPYLRLTPQPNK"
     gene            16895..17308
                     /gene="ruvX"
                     /locus_tag="PLS16_02325"
     CDS             16895..17308
                     /gene="ruvX"
                     /locus_tag="PLS16_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNJ59942.1"
                     /translation="MPKILSIDYGTKRTGFAISDDSKIFAFGLSTQETSNNLKYIQNI
                     ITKENVDTILIGIPKRLNNELADIVPKIEAFIDKIQQQHPQIIIERLDERFTSKMAFQ
                     SMIDSGMKKKDRRNKALVDEISATILLQNYLEYKN"
     gene            17332..17895
                     /gene="def"
                     /locus_tag="PLS16_02330"
     CDS             17332..17895
                     /gene="def"
                     /locus_tag="PLS16_02330"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNJ59943.1"
                     /translation="MILPIIVYGSPILKQKASEIDAEYENLAELIQNMWQTMYQASGI
                     GLAAPQINKSIRLFIVDTAQLEDRKKDEPNFVGIKKVFINPQITEEIGAEWKYEEGCL
                     SIPGIRENVERKPDITITYQDEQFNTMTESYSGLNARVIQHEYDHIEGVLFTDKVKPL
                     TKKLIQSKLNKIMRGDFRADYKTKSVK"
     gene            18476..19204
                     /locus_tag="PLS16_02335"
     CDS             18476..19204
                     /locus_tag="PLS16_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNJ59944.1"
                     /translation="MNDINQHIQLIKSRIESACHKAKRNSSEVRLLLATKTVSPENIK
                     IAIASGETLIGENKIQELKEKFEDLQRIPHEKHFIGHLQTNKIKDILKYGVTCVQSLD
                     RIELAEKLHQRLSVEKKQLDVFIQVNTSAEESKFGVTPEQAIELIKSVAKFDTLKIKG
                     LMTIGLFSAETEKVRPCFKILKNLQQEIIAQNIPNVDVQELSMGMSGDLETAIEEGAT
                     IVRVGTAIFGQRIYPDSYYWNEQA"
     gene            19427..19870
                     /gene="rplM"
                     /locus_tag="PLS16_02340"
     CDS             19427..19870
                     /gene="rplM"
                     /locus_tag="PLS16_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020063210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNJ59945.1"
                     /translation="MKRLSYKTRTVVPSESDRTWYVVDAENKTLGRISSEIAKVLQGK
                     NKPSYTPNIDNGDHVIIVNAEKVALTGNKLTQKVYIRHTGYPGGQRFMTPKEALVKKP
                     TFILENAITHMLPRTRMGRVMAKKLHIYVGAEHPHAAQNPKALNI"
     gene            19890..20282
                     /gene="rpsI"
                     /locus_tag="PLS16_02345"
     CDS             19890..20282
                     /gene="rpsI"
                     /locus_tag="PLS16_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004695095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNJ59946.1"
                     /translation="MANTSTNGLGRRKTAVARVFLKPGTGVITINSKPADQYVSLKYL
                     LDRAVKPLHVVAQENNFDVNINVKGGGIKGQTEAISLGIARALIKIDEANKPALKAEK
                     LTTRDPRMVERKKFGKKKARKSFQFSKR"
     gene            20319..21059
                     /gene="rpsB"
                     /locus_tag="PLS16_02350"
     CDS             20319..21059
                     /gene="rpsB"
                     /locus_tag="PLS16_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNJ59947.1"
                     /translation="MSKISQQELLDAGVHFGHLKKKWNPKMLPYIFMERKGIHIIDLN
                     KTQEKLEEAGAVLKSIAKSGRKVLFVATKKQAKDIVEEAAKSVNMPFVTERWLGGMLT
                     NFATIKKSIKKKQNIEKMLADTNLNSITKKEKLILSRQQQKLDKVLGGIENLPRVPAA
                     LFMVDISHEHLALAEAKKLGVKTLGMVDTNSDPNLVDFAIPANDDATKSIQIIVNYLT
                     AQIKEGLAERKKDKEETESTGEVAITKE"
     gene            21111..21947
                     /gene="tsf"
                     /locus_tag="PLS16_02355"
     CDS             21111..21947
                     /gene="tsf"
                     /locus_tag="PLS16_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNJ59948.1"
                     /translation="MSVTITAADVNKLRQETGAGLMDCKKALVETNGDFEAAIDYLRK
                     KGAKIAANRAEREANEGVVVALTSEDGKFGVAVNLCSETDFVAKNEDFINLAKNITQI
                     ALDNRVTSAEALEACTIDGVTIKDKLLDTVAKIGEKIEVKKFDFIQGEGVVAYIHMGF
                     RMGVLVELNKAINESNAVVGKDVAMQIAAMNPVAVDASGVSEEMKERERAIAKEKAIE
                     AGKPENIIEKIADNAVNSMIKENTLVNMPFVKDSSKTVEQYVKSEDKDLQVLSFRRVT
                     LS"
     gene            22000..22290
                     /locus_tag="PLS16_02360"
     CDS             22000..22290
                     /locus_tag="PLS16_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59949.1"
                     /translation="MIRIFYTLLFVVLLSLLLPVACTKKKSECADCFGIVEAFNSEII
                     KWNFDSVKYRNPNAIESQWCEILDQIDGKPVQDKNGNTIGTFRKECPTITTP"
     gene            22325..23035
                     /gene="pyrH"
                     /locus_tag="PLS16_02365"
     CDS             22325..23035
                     /gene="pyrH"
                     /locus_tag="PLS16_02365"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNJ59950.1"
                     /translation="MSKPIKRILLKLSGEALMGDQNFGIQAQTLDSFAQEIAEVLEHG
                     YEVGIVIGGGNIFRGLQAGNIGIERSQGDYMGMLATVINGMALQGALESKGVYTRLMS
                     ALNIQQVCEPYIRRRAVRHLEKGRCIIMAAGTGNPFFTTDTAAALRANEIEADVILKG
                     TSVDGVYSADPKKDSSATKYETIGFNEVLVKNLRIMDQTAFALCRENNMPIVVFNIRE
                     KGNILRILQGEKIGTLIS"
     gene            complement(23103..24719)
                     /locus_tag="PLS16_02370"
     CDS             complement(23103..24719)
                     /locus_tag="PLS16_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNJ59951.1"
                     /translation="MVTVQNLSLQFGKRVLFDEVNIKFTPGNCYGVIGANGAGKSTFL
                     KILSGDIDPTTGQIEISPGERMSILKQNHFEFDEEQVIDVVMMGNQKLYSIMKEKDAI
                     YAKADFTDEDGLKVGELEAEFAEMDGWNAESDAAELLSNLDVVEDNHYKLMKELPSNQ
                     KIKVLLAQALFGNPDILILDEPTNDLDLKTITWLENFLADFKNTVIVVSHDRHFLDMV
                     CTHIVDIDFSKVNLFTGNYTFWYETSQLLLKQRADKNKKNEAKKAELLDFIARFSANA
                     SKSKQATSRKKALEKLDIEQIKPSSRKYPGIFFKQTREAGDKILDVHNLAYKNEDGKT
                     FFSNVTFTINKNDKVAIISDNSMAVTTFLRILAGETQANSGSFEYGVTITKSYIPNDN
                     KSYFEVDLNLIDWLRQYSTEKDETFVRGFLGRMLFSGEETLKKCSVLSGGEKVRCMLS
                     KTMLEEGNFLILDEPTNHLDLESITALNNGLIDYAGTMIFTSHDHTFTHTIANRIIEI
                     TPKGVIDRLMSYDDYLADEKIQALRQEMYA"
     gene            complement(24799..25608)
                     /locus_tag="PLS16_02375"
     CDS             complement(24799..25608)
                     /locus_tag="PLS16_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNJ59952.1"
                     /translation="MDIFQAIIIAIVEGLTEFLPISSTAHMIFTSTFFGIQEDEFVKM
                     FQVSIQFGAILSVVVLYWRKFFNFKSFDFYIKLALAVVPALVLGKLFDDKIDELLGSP
                     IPIAIAMILGGVVLLFIDNVFKNPIIEKEEDITVKKAISIGFWQCLAMMPGVSRSAAS
                     IIGGMQQKLTRDVAAEFSFFLAVPTMAAVTLYSVFLKDWGTGENVQKGYEMIAQSGNL
                     SIFIIGNIIAFLVALLAIKTFVEVIKKYGFKFFGYYRIFAGIVILILLLVK"
     gene            complement(25615..25971)
                     /locus_tag="PLS16_02380"
     CDS             complement(25615..25971)
                     /locus_tag="PLS16_02380"
                     /inference="COORDINATES: protein motif:HMM:NF022741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HNJ59953.1"
                     /translation="MAKNKPLQNNPKPKVAPISQPKKPISKSSNSGSFFKIPQSTNTT
                     ILFDKINYILIGIGGILIILGFIFMSGGNQDPKVFNEAELYSFRRITLAPITILLGFA
                     VIAVAILKKPKTQITE"
     gene            complement(25982..26857)
                     /locus_tag="PLS16_02385"
     CDS             complement(25982..26857)
                     /locus_tag="PLS16_02385"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNJ59954.1"
                     /translation="MAEEQKYKPKKRYQPTYKYAILTIGLNLFIIGLFAIIIYYSNAS
                     LIKLKQSFEIELILDDSLQNSNIDAIKATLQKKDYLHKINFRSKEVAAKMYEKELGQN
                     FTKILGYNPMYNAFIVNLKAEKADKSSIKAIKSDFLSINGIKEVNYSEPISDMFGNTV
                     RPLFIGILAISLILFIIAFSVIDSTIRLMMYTQRYAIRTMQLIGGTKWFIIKPFLIKA
                     ISAALISTFIAILILVGIVFFFTDKYSIQLVQMDYLVLAGIGGGLLLTSLLISLISTF
                     FAVNKYWNIKLDDLF"
     gene            complement(26901..28064)
                     /locus_tag="PLS16_02390"
     CDS             complement(26901..28064)
                     /locus_tag="PLS16_02390"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNJ59955.1"
                     /translation="MITINKRIIGLDALRSFALFMVLFGHNATLFNPLIHLPIIGNLA
                     GKALAIHEIIGFSGVEIFFVLSGFLITDIVFKYFNNNRNNFAQLKLFLIRRWLRTLPN
                     YYFMYIVNTILYVWILKEKTFDWKFILFIQNLNYPHPDFYREAWSLATEEWFYCLLPI
                     ILIAIYNFKKLDFKNSILLTCLLMFLFSFALKIFHIQQYGIHLNFDENIRKVVLLRFD
                     NLIIGILCYVCFYYYNTLFLKYKNWLFWFGLLLIIPTVPLFYAAYQNKFYLYHHNNLI
                     AIYCNFIFLPTLGFITALIIPKLYYLNLDKQKYLSAFFKKSSQLAYSMYLINLPIYKL
                     FFFRNNLIPDSIGSSIMYILLFLLINYGIAQLIYKYIESPFLKYRDRKYPNVY"
     gene            28115..28789
                     /locus_tag="PLS16_02395"
     CDS             28115..28789
                     /locus_tag="PLS16_02395"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNJ59956.1"
                     /translation="MKNAPFGIELYLYPTYFIDCNHPDVIEYAHNIIGDEKDKKQQAI
                     KLYNAIRDGFRYNPYNINLKHEELKASTLLNRASKSGYCTEKACLLAACLRVVGIPSR
                     MCFFDVRNHIGVDKYIEIIKTDVLTFHACTDVFLDNKWVKATPAFNKTLCDKLGVAAL
                     EFDGENDSIFQEFDSKGNEFMEYLHDYGTFHDIPHDMFVAQMKKHYGHFFENLEATKA
                     MVNDSE"
     gene            complement(28791..29768)
                     /gene="ftcD"
                     /locus_tag="PLS16_02400"
     CDS             complement(28791..29768)
                     /gene="ftcD"
                     /locus_tag="PLS16_02400"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HNJ59957.1"
                     /translation="MFSHSDILIECVPNFSEGRNKATIDAIAEEIRQTPSVQLLHIDI
                     GFDANRTVYTYIGKPEAIVEATYKAIKKAYKLIDMRLHAGKHPRMGACDVCPLIPYQN
                     IDMETTKTYVEKLGKQLGDLGLPVYLYENSATTEARKNLADIRKGEYEAIADKIIQKE
                     WKPDFGPTVFNQKFGMMALGARALLLAYNINLKTKDLSIGKKIAKAIRDKRDNEKYPQ
                     LQYCRAIAWYIEEYGFVQISTNITNYNASPIFYIFGTVKEIANDFGIRVNGSELIGLI
                     PQKALLVQNPSNKISDQAKIEDAIEYLGLNQVFSFQKEKRIIDYILELK"
     gene            complement(29847..>30489)
                     /locus_tag="PLS16_02405"
     CDS             complement(29847..>30489)
                     /locus_tag="PLS16_02405"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNJ59958.1"
                     /translation="SISYIRNKLIHVKTVISFGIPSILGVLFTRAYIIPNIPEKIITI
                     NGFTVTKDLFIITFFAILMAIASYSMIKKSNDNEVKERKKLNINYTLISIEGLVVGII
                     TGLVGTGGGFLIVPTLVILLKLPMKEAVGTTLLIIAAKSLIGFLGEPNIAGMDWKLLI
                     SVVSCTISGVLIGTFLSRRMDGRKLKPFFGWVLLILGLSIIIKEIFMNRLLVS"
BASE COUNT        11150 a         4304 c         5115 g         9920 t
ORIGIN      
        1 acagtaacag ctgataacgg aagttgtaca tctacttcag caacctttac tatagctgga
       61 caattaacaa caccagcaac accaacagtt agtgtaacag caccaacatg tacagatgca
      121 ggttttgcac aagttaccaa ttatgatggt accttaaatt atacactatc atctaataat
      181 ataagtatag atggcagtgg taaaattacg ggagcagcag gcaattacac agttaccgta
      241 ggaaatggtt catgtagtgc gacttctgca tcatttagta tagcatcaaa attagtagtg
      301 ccatcagtac caactgtaaa tgtaattgca gccacatgtt cgacagcagg ttcagcacaa
      361 gtatctaatt ataatagtag tctagtatat acagtatctc caaacacggc gaacatagat
      421 ggtagtggaa agataacagg agctgctgga ccttatacag taacagctga taacggaagt
      481 tgtacatcta cttcagcaac ctttactata gatgctttaa tatcgtcagc accaacgcct
      541 gtgttatcag cagataatac gacaatatgt gcaggaaata gtgctttatt atcctctgat
      601 gttacaacgg taaaatggta tagagatgca gccaaaacaa atgttttagg tactggaaca
      661 cctattagta ctggtaactt atctgcaacg acaacttttt atgcagaaaa tacgtcagga
      721 gcatgtattg gagcattagc atctataaca attaatgtag tagcagcacc aagtgcacca
      781 acagccacta aattaccaaa taaatcaagt gtttgtatag gtacaacatt aacgattcaa
      841 aatcctcaat atggtaccaa tagtggaaac agttgtccat ttgagtatca atcaagtaca
      901 gatgcaggac aaacgtggag tgctgtttca tcaacagttc caagtttgat tgcagatggt
      961 acagaaaata taataagaat tagagtttca gcatgtaatg gatgtacagc aagtaattgg
     1021 acagattatt cttggacagt aatttcagat accgcaagac ctgcaattac aggaaactta
     1081 tctgtttgta caggaacatc aacgacctta gttgcaaatg ccccaattgg ctcaacagtt
     1141 cgttggtatt cagataatgg attaaccaca ttagtgaata caggcacatc attctctcca
     1201 attatcacat caaatacatc attttttgtt acacaaactc aagatttatg tacaagtgcg
     1261 gcaactcagg tagatgtagt agcacttcct gcaccagcag caccaacatt aacgttagga
     1321 cattctccaa tatgtggagg tcaaaacaca gatttatatt ctagttcatc aacagtagtt
     1381 tggtatttag atgctgctaa gtctaataga ataggcgttg gctccacaat aaatacagga
     1441 actatctata caacaatgac atactatgca gcagatactt caggtttatt atgtccaagt
     1501 ccattgtcat caattacagt agatgtaata ccggcaccag atttaggaaa tatagttgat
     1561 gtaagcattt gtagcggcac atctgcatta ttaacaggag gttcatctgc gactgaatgg
     1621 tatagtgatg atgcaatgac taataatatt aatatgggag caacttatca aacacctata
     1681 ttaacagcaa ctacaaatta ttatttaaga gtaccaagta gctgtggagt tactttagat
     1741 acagttacag ttacagttaa accaatacca gcagcaccaa cagcaaatga cgtagaagta
     1801 tgtagtggtt tatcagctag cattgcttta gccaatgtaa gtcctggtgc gactgttcag
     1861 tggtataatg ctccaagctt agataatatg attcatgttg gttcagtttt aattacacca
     1921 atattaacat cagacgctgt ttattatgta acccaaacag tagatggttg tacaagtcca
     1981 tcaagttcgt ttaatgtata tgttaagcca agtagtaatg ctccaacatt aactgcaaat
     2041 ccaaatgtta tttgttttgg agacgcttca gcgttaagtt cctcttctac tgcaacaata
     2101 tggtatagtg atgctggtca aacagttatt ggaaatggac aaacaatttc agtttctcca
     2161 ttattaacca caacatacta tgctcaagat acagcagtag gttgtagtgc attagcaagt
     2221 gtaactgtaa aagtagattc tatattacca gcaccaaata ttcaagatgt tacagtttgt
     2281 caaggagcag atgcggtatt aactggaggc aattctggta cagcgtggta tgcagccgat
     2341 caaacaacac taattggaac aggagcaact attatagtaa cgaatgtaca atctgcaagt
     2401 ttatattatg caagaaatga aggaagttct tgtgtcagtg catttgatga tgtaactatt
     2461 catatcacaa caacgccacc accgacagca gcagatgcag aggtttgcag tggtcaatca
     2521 gcaagtttaa gtgcaactgg tcaaaatatt gtttggtata gcgatgcagg tttaaccaat
     2581 gcagttcaaa gtggctcggt attaataacg ccagcattaa caagtgatgt tacctattat
     2641 gtaacaaatg agtcaaatgg ttgtgagagt gaggcaacag cagttcaagt aacagtaaca
     2701 acaccagcac cagcaccagt aatcacagcc aaccctacaa gtgtatgctc aggaggaaca
     2761 acagaattaa gttcaagtgc aggagccaat accgtatggt atagtgatgc cggaatgact
     2821 gtagtaggaa gtggatctcc atttacaagt gcaccattat tagctacgac aacgttctat
     2881 gcaagagata atagtgtacc aaattgtcct ggagaattag caagtgtaca agtattgata
     2941 agtgcatcag aaactgcacc aaatgtatcc aatacaagtg tatgtgaagg aagtagtgca
     3001 acattgactg gtggtacagc gaatactcac tggtatagtg atgctagctt gacaacagag
     3061 gtaaatgtag gcacaaatta tacaacgcct gtattatcga ccgccactac ctattattta
     3121 agaaacgaaa atggattatg tgtaagccca gtaaccgaag ttactgtgag tgtaaatccg
     3181 ataccagctg caccaactgc tgatgttgtt gaaccaatat gcggaagtgg aagtgcaagc
     3241 ttatcagcac aaggagatgc caatgcaata attacttggt atgcagacgg tgcaatgacg
     3301 aatgctttac atataggttc aagctatatt acaccaatat taacagcaaa tacaagttat
     3361 tatgttactc aagagttgaa tggatgtgag agtgcagtaa cagaagtgga agtagaaatt
     3421 aatgaattac caacgataga tccagtagat cctcaacaag tttgttcagg aagtacagca
     3481 atttttgaag cggataatcc aaatggctat gatattgagt ggtattcaga tcccgcaggt
     3541 ttagatttat ggcaaattgg agatgagttt gaaactccag ttcttacaca atatacagca
     3601 tggtattata gagcagtaga tccaattaca ggttgtgagg gagaaattca atcaacttgg
     3661 gcagatgttg tatctaataa agacataaca attacagcga cttcaaaacc aacatgtgta
     3721 gatgaagata tcgttgtaaa tgttgcatct tacgatttct ctggtttagt tggtattttt
     3781 gattatgatg gaaaccttgt tgcagttgaa gatataaatg gaactacagg cggaacacaa
     3841 gtaattttac ctggtttacc agtagctggt gtatatagtt attatgtgct agaagcagga
     3901 gatatattct gtgctacctc atcttctaca tttacagtcg aagttgtaga tagaccagtt
     3961 attgataatg tagttggaga tgaaatatgt ataggagagg tcgctcaaat tacagctaca
     4021 gccacacctg caaatgcgat aattacatgg tatagtgatg ctacactagc aaatgcagtg
     4081 ggaacgggca atacattaac aattcctgga ttaacagtaa atacaacata ttatgcagtt
     4141 gcatcaaatg gtttgtgtgc tagtgatcca atggatgttg aagttattgt aaatccaatt
     4201 cctgcaacac cagagctaag ttcaaattca cctgtttgtg aaggaagtga acttacttta
     4261 tcgacaacaa cagctggaaa tgtaacttat atttggagtg gtcctgcggg atttttatca
     4321 ggagaccaaa atccagtgat agcttctgta actatggcaa atcagggatt atatagctta
     4381 aaagtacagg ataatgtaag tggatgcaca tcattaacag cgagtgtgca agtaaatata
     4441 aatacagctc caacagctcc aacagctagt aatagtgggg caacttgtga aggaggctct
     4501 gctattttat cagcgacgac aatcccagga gctacttatg aatggacagg tcctaatgga
     4561 tttagtggtt cacaacaagt tataacatta aatcaagtta ccttagcaca agcaggaact
     4621 tattccgtaa gagcatttgt aaatggatgt tggtcaagtg caacaactac agatttggta
     4681 gttaatgaaa aacctatagt tagcatacca gatgtaaaac cagtgtgtga aggtggtaat
     4741 ttagaaataa tagctacaac caatagtgca aatccagttt atgattggag aggcccaaat
     4801 aattatgttg gacaaaatgc tactgtaacc attcaggatg tgaaggaatc taaacatcaa
     4861 ggagcttata ctttacaagt aacagatgga acaacgggct gtaaatctga tgtagttgcc
     4921 acatgggtag ttattaatga aaaaccaaat aacattatgg ctataaataa tagtccaaaa
     4981 tgtgagggag gttctgttca attaaatgca tcaaatatca ttggagcaac atatacttgg
     5041 acaggccctg atggatttgc ttctcaagat agaagcccta taattacaga cttaaatgct
     5101 aataaagttg gtacttatac attgacagta acattaggag attgtttatc tgagacagtt
     5161 acaacaacag tagagatgaa tgataagcct gttgcaaatg caggagatga tataacaaca
     5221 caaacaaatg taccagttaa gttgaatggc tcaggaggaa tcatttatgc ttggtctcca
     5281 gcaaatatgt tatcattctc caatatccct aatccaacgt ttattgcagg ttcagtgggt
     5341 gtatatgaaa tggaattaac agtaaccaat gaatttggat gctctgatgc agatacagtt
     5401 gttgtaaatg ttgaaaaagc aacaaccaat ttagatagtg tagtgattcc tgatttaatt
     5461 acaccaaatg gagatggtat aaatgatgta tggagattag actttttgaa ggatatcaat
     5521 atgtcttatg agattgcggt atatgcaaga ggaggctcta agattatgat tagtaccaat
     5581 tataataacg attggacagg aacatataaa gggaaggaat taccagatgg ggtttattgg
     5641 tatgcaatcg tattagaaaa tggtgctgtt tataagggtt cattaactat taaaagataa
     5701 gcgatagaaa acaaatagaa aataaattaa aagagaaaag aatatgaaat caattaaata
     5761 tttaatcaca tgtttgtttt tgagcttaac ttttgttgtt tcaaaagctc agcaaacacc
     5821 agtatataat aattatacaa taaatccttt tttatacaat ccagcaagag caggagataa
     5881 ccctgatggt ggcgtgataa atctaggatt tagaaagcaa tgggatagaa taccttatgg
     5941 accagttaca ggaaatgcga catgggattc tagaattaag gaatcaaata tggctcttgg
     6001 agcttcactt acacatgata gagtaggtaa gcatattatg aatacgatgg caagttttac
     6061 atataactat agattgccat ttaataaaga aaagaatcat ggtttgaatt ttggtattca
     6121 agcaggaatt atctcacaaa agtttaattt tacagatgca attacaacag atggaatttt
     6181 taatgataat gcagcagtaa cgaataattc aagagcaaca aaatttgacc tttctttggg
     6241 tataaattat cattggaaag gtttgaatgt tgggttttca gtgcctcaat tgttaaatgg
     6301 taaagctaag tttagaggaa atggaatgga taatgaaaca agtatgtttc attatgaaag
     6361 agaatattta gtacttgcaa gttatgaagc aagatttgga ggcaaggaca aaaagacttg
     6421 gatgataaca ccatccttaa tgtttagaaa gtataaatca atagacccac aaattgattt
     6481 gaatgtaatg tttggttata agcaattagt ttggttggga gcaggttata gaacaggagg
     6541 tggttatggt ggatttatta aaacaaactc agcaggattc catgttacag caggagctgg
     6601 aattaaggaa agagtgaatt tattctattc atttgagatg ccgatgaaag atgtaagatc
     6661 aaattttggg tatacacatg aagttacgat acagtgtaat ttagcgaaga aagtcgataa
     6721 gtctgaatat gaaagaaaca agaagaaagt agaagatgat atagctaaat taaatcaaaa
     6781 gacagatgaa gttggttcaa aagcaataga ggcaatggat aaaggaaatg atttagcaga
     6841 tcaagtgaag aatgcggata attctataaa agaggccttt gagaaattaa atgcacagga
     6901 agataatata aaagagattg cgagtagatt agataatatc gtgtttaaga aatttggttc
     6961 tatttatttt gagaatgata agtctgaatt aactgctgag gcaaaagcga gtttagatgc
     7021 tttcaaatca aaattagcag gaatgaaagg taattacttt atttatttag caggcaatgc
     7081 ttcacaagaa ggagcgacag attataactt agcactttca gcaaaaagaa gtgatgctgt
     7141 taagaaatat ttagagggcg taggtatatc acagagaatt ttattgttac catatggaga
     7201 aaacagttgg gtaatagaca aacaagataa tgaggcagac aaagcaaaaa acagaagagt
     7261 agatatctac ttagctggag aatagttgtt gtttgttagt gaaaataaag aggctgtccg
     7321 aaattattgg acagcctctt tttatttgag aggaatagag gcaattccat tttagaatta
     7381 atagtattat ataaaagtcg ttttgaggta ctcattattc taaatttaaa gctaaattca
     7441 tttaatattc ttacactcat ttataatccc tattgtaagt gttataatta cggtgtgaaa
     7501 attattaaaa cttcaacaat ttttataatt tgacttttgc attggaatcc acttgtaaaa
     7561 aatggagttt ttagatattg gagagacatt agcatttctg aagaatcgaa tagattattg
     7621 tattcgaata tttagtatcc aaatttgtat agctttagtt agaacaagtt tgtaagatta
     7681 atcagaattg cctcagaatt ctaggaattc aaattatggc attatacaga aaaatagttg
     7741 aaaaattctt taatctttgt agtatttcac taacttttag ctcaaaatta ttgagccttc
     7801 aaagccattt gctttgctaa tttctttctt cttgaatatt ctttaaaaga aatcattttc
     7861 ttttgctctt catcccaaag attagaatgg attgttttta aactttgcca aaatgtaatt
     7921 gtattggtgt gtttagctaa aattggattt tcctggtggc attttcttaa attataattt
     7981 ggaatagaag aactcaaatg gtgtatatgg tgataaccga tattgccaga taaccaatgg
     8041 aaaatccttg gtaatttgta ataggttgtc ccttttattg ctgcaacaac ataattccag
     8101 ttttcttttc cacttttata aataaattcg tattgatgtt gaatataaaa aaaccataaa
     8161 gcataaattc caaaaaagaa taaattagta aattgaacca ctagaaatct ttcaggacct
     8221 aatatataag cagcaattgc gtaagtaata ataaatatag cattactaat atttacattg
     8281 ttttttactt ttttgaaata acttaaattt gaaaaagcaa aacgattata tactacaaca
     8341 tatataaagc caccaattgt aaatagataa aatgggtttc tatatacacg ataataaatt
     8401 ttagttttcc aatttgcttt cacatattct tctacagtca tacaatcaac atctccaata
     8461 tcacttactt ctaattgtcc gttgtgtgca tggtgaaatc cgtgattttt tgcccaatat
     8521 ttatatggaa ttaatgtgca aatactacaa attgtgccaa taatcgtatt ggctttcttg
     8581 ctttttaaaa atgaactgtg tccacaatcg tgttgaataa taaaaattct tgaaagaata
     8641 aagccattta atagagcaac taatgcagac caaaagaaag atttttcata taagtaaaat
     8701 tgcaaaccta ataaaagaac ataaaatgaa aaactatttg ccatttgaat aatagcttct
     8761 tttgtattag gaacttggta tttctttacc atagattgcc aatttttcaa atcatctaat
     8821 atttgttgtg agttaaagtg tttcattagt ttgtgcttgt tatttagact atacaaaaat
     8881 aagcaatctt gtgtagtcta atgttgttta tttctttaat taataagaat ataacaaatc
     8941 attatgcact caatcacaat gtcaaggtca ttgaattatt tatcataata aattatatca
     9001 gcacgtttcc atctgcggtg tgtccaaagc caatatgcgg gttgtttttt tatatcttgt
     9061 tccaaatttt tagcaaatcc atcaacaata ttttcaatac tttcgttagc agaatttttt
     9121 gtaatttcct ttagcttgat ttcaaaatgt gtattgttga taggttcaat ggatgcatat
     9181 aaaattacgg cattaatttt ttgtgctaaa tgagcatagc cattgtaaaa tggtgtttcc
     9241 actccaaaga aattaatcca agtagcttta ttcacagcaa caggagtttg gtcggcaaca
     9301 aagcaaaaca tagaattatt attgtctaaa tttttaaata tatttttgat aagtttagtt
     9361 tcaatcaatt ctattccaaa tttggctcta tttttcttaa cgaatctatc tattgtttgg
     9421 ttgcttaatt tttgatagat accataggtt ttgaacctag tttccatggc ggctaaaaat
     9481 accgataatt cccaatttcc aaaatgagaa gtaacaataa aaattggctg attatttttt
     9541 tctaaagccc aaattaattg ttttgcgtcg tggtgtaacc aaatatattt ttttacttgg
     9601 tgttccttaa aagaatagga ttgtaaattt tgggatatta aatgggcaaa gtatttataa
     9661 aattgctttt caatttgctt taattcataa ctacttttat ccgaaaatgc agtcattaaa
     9721 ttaatcctta cggtggttac tcgatatttt attattttat aaatgagtat atagattaaa
     9781 taacttatta ttccaaaaaa atgattagat agaaaagaca ttatcttaat aaatattatt
     9841 gtgatatagc caattgtctt ttttatcatt tatgatttga tttatagtaa tcttgtttac
     9901 aaatatatca ttcttttaat ttttgaatag tgctaggagg attatttatt ggacataagt
     9961 ttattttctt aaatttacat ctaaattaat agagaaatga aaattgctga tattattgct
    10021 tgcatagaag attttgcacc taaaagttat caagagtctt atgataatgc tggtttaata
    10081 attggcaatg caaaaaatga atgtacagga gttcttattt gcttggatag tatagagtca
    10141 gtaattgatg aagcaattgc caaaaatatg aatttagtgg tggcacatca tccaataatt
    10201 tttagtggaa ttaaacagat tgtaggaaaa aattatattg aacgtattat tattaaagca
    10261 ataaaaaatg atattgcgat ttatgctgca cacacgaatt tggataatat gcaaaatggt
    10321 gtgaatgcta ggataggcga aaaattaggt attaaaaact caaaaatact tcagcctaaa
    10381 agtaaagtac ttaaaaaact ttatacctat attcctacaa cacatacaca aaatgtagca
    10441 caaaaactat ttgaagctgg agcaggaaat attggaaatt atagcgaatg tagttttaca
    10501 atcaatggta aaggaacatt tttgccaaac gaaaatgcaa atccaacact aggaaaaaaa
    10561 ctagagcgtc atactcaaaa tgaagataag ttagaagtgt tatttccttt tcatttagaa
    10621 caaaaaattt taaatacctt atttcaaaat catccttatg aagaagttgc ttatgaaatt
    10681 attacaatag acaataaaaa ccaaacaatt ggttctggaa tgataggcga attagataaa
    10741 ccaatgaatg aaactgattt tttatatttt gcaaaacaac aattgaatac aaaatgtatt
    10801 agatacacag cacttcaaaa taagccgatt caaaaagtag cgtggtgtgg tggagctgga
    10861 aatttcttgt taggagatgc tattgctcat caagcagatg tttttatttc aggtgatttt
    10921 acttatcata aattctttga tgctgataat caaataatta taatggatat tggacattat
    10981 gagagtgagc aatttacttc tgaaatattt cttgatattt taacaaaaaa atttcgtaat
    11041 tttgccattc aaatttcgga aaataatacc aatccaataa attatatata aaatggcaaa
    11101 gaagaaagaa cttacagggg ttactgataa attagaacaa ttagcaggat tacaagctat
    11161 tcattctaaa atcgaccaaa ttaaagtttt aaaaggagaa ttaccaattg aggtagaaga
    11221 tttggaaaat gaaatttctg gtttagaaaa aagaattact aaaatcggaa atgaactaaa
    11281 agaaacagaa gataacatta ctcaaagaaa gcaaactata aaagatgcta ctaacttgat
    11341 tgcaaagtat gataagaaat tgactaacgt aaaaaataat agagagtacg atgctttgaa
    11401 taaggaagta gagttgcaaa aattagaaat ccaattggct gaaaagaaaa ttaaagatgc
    11461 aactttttct ttagaaagtt caaaaactag tttggaagag gcagaagcta aacacgctca
    11521 aaaaatctct aacttaaatt ctaaaaagaa agagctttca catattattg aagaaacaga
    11581 gaaggaagaa aaagaattaa gtgcacaagc atcagaatta gaaacacata ttgaagaaag
    11641 attattaact gcatatacaa gaattcgtaa gaattataaa aatggattgg cagttgtcaa
    11701 tgttgaacgt aatgcttgtg gtggttgctt tggatttgta ccagcacaaa ctcaagcaga
    11761 aattcgtact aaaaaacgta tcatctcttg tgaacactgc ggaagaattt tatacggtgt
    11821 aaaagatgat gttgaacttt ctgctgaaga aatcgcatag ttttattttt ttaaataata
    11881 aataacattt ttagtgttat tttggacagc ataatatgtt gctaaagcca ctaaaatata
    11941 tcattgccat aatttacgaa gcaataattc gctttagatt tagattgtat aaacatcatt
    12001 ttttaggtca aacatcgttt gatattccaa ttatttgtgt tggaaatttg agtgttggtg
    12061 ggacagggaa aacaccaatg gtagattatt tatgtacact tttagaaaat caatttgcaa
    12121 taggcattat tagtcgaggt tataaaagaa aaaccaaagg ttttatccgt ttaaaagcaa
    12181 agcatacagc aactgaagta ggagatgagc ctttactttt aaagttaaaa cacccacaaa
    12241 tagaaattgc agtgggagaa gaaagaattt atgccattcc acatctttta agcgatgcac
    12301 cacaaacaca aattatactt atggatgatg ggtttcagca tctgagtgta caagcacagc
    12361 ttaatatttt aatgactgat tatcaacagc ctttttggtc cgataaaatc ttaccattag
    12421 gtactttgag agaacctaaa gaaggctaca aacgtgcaga tattatcatt gtaaataagt
    12481 gtcctgataa tataagcaaa gtggatatgc aaacttgtat tcataaaata gaagcattgc
    12541 ccaatcaaaa agtattcttt gctaaaatta aatatagaaa cctatatgct ctgttttata
    12601 atatagttcc aaatgcagat gaaaatccat atattttatt tacaggaatt gcaaatgctg
    12661 aatctatttt tcaatactta aatccaaaat ttagaatttt tcattttcag tttgatgacc
    12721 atcatgctta ttctatcaaa gattttgaag atatgtatgc tgaaattaaa acgaacaatt
    12781 ggataactac tgaaaaagat gctgtaaaat tattcccttt taaaacttgg tttgaaagca
    12841 aacaaatcaa tgtatgggtg caaccaattc aattagaaat atcttcgata gataatgcct
    12901 cttttgatga attaatacat caatatttag actattattt ccaaaccaat actgaaacta
    12961 attgatgcat aaagtgtatt tttgtaaaat gtgtcaaaat gtgctaggtt ttttgatgat
    13021 tagcttttta tttgctttca atgcaaatgc acaaactcgt attacgacac ctaatcctat
    13081 tcttcgatac gatacaaaat atattacgtt agataatgat gagcttcgaa agctagattc
    13141 taataaccat aattttcatc gttataatgt aaccgaaaaa caatcagtac cctattataa
    13201 tttaggaaat tatggaacag cttattatcc tttgatgttt cagtacaata gaaatattgg
    13261 attttcaagt ggatataata gctttgattt gtataaacga aataaattag atattcaatt
    13321 ttttgataca aaaacacctt atacagactt agattttatt tttggtgcaa aggaagaaat
    13381 tactggtggt gctgactttt caatgaatgt aaatccacaa gttaattttg gtttatatta
    13441 tagaagaaat agttttaaag ggcaagcaaa attgcaagaa agtgtttata ataattttag
    13501 tttgaataat tggtacagaa gcaaaagtaa tcgttatgaa ataaaatctg tattcttatt
    13561 caataatgta aaaaatcaag aaaatggagg ttgggcagtt ggaaatattt acaatgatgc
    13621 tatatataaa gggaaaaaaa ataagcaatt tgctgatgta aatttacaaa cggcaactca
    13681 aaaatatact gaaaaaaatg tattttttat ccaagatatt cattttggga agaaagagat
    13741 tgttgaagta aatgattcta caaagcaaac taaaattaca ccaagatata cattagaaca
    13801 tgagttttct tataaaaagg aaacctattt gtttaaaaac tacaaacaag attcaacatt
    13861 ttttcagcaa cattatttaa gtacagattc cacacacgat gctactaaaa cttggaatat
    13921 cagtaatagt ctagctttta aaaatgtagc tgatagtaca actaaaattc catttttata
    13981 caaggttggt gttcaattgg atttgattaa atacaagcaa ttctataata acaagttcat
    14041 tacagatatt aaactttttg ctgaaataaa gaacaaaaac aagcaatcta aattacaata
    14101 ttttgcaaat gcaatgattg atattgcacc aaaatatatt ggagattttg aagtagattt
    14161 aggtgcaagt tattttataa ataaagaaat tgctattgaa ttgactaata aaattcaaca
    14221 tcgttcacca agtgaaaaag aaaacttttt agtttcaaat cattttttat ggcaaaataa
    14281 tttcaagaaa acattccata atcaaacgaa tttagttttt aattgggcta aacaaaagtt
    14341 ttatatcaac tttgaaaatc atatcctcaa aaattatatt tactatgata ccgcaacaac
    14401 attacaacaa tataataaaa atctgattgc gtttaatgtt caaattagaa aagattttga
    14461 gtttaaaaat ttttatatag gtaatacttt ttgggtccaa ttctttaata ataaagacat
    14521 cattaagcta ccaacttttg caatgttaga aacattttac tataaaggtg gttggataag
    14581 tggtaagttg aacgcacatt tgggtgtcaa tattagttat aataccaatt ttaatacacc
    14641 aaaatatcaa ccagctctag ctgaatttta tattcaaaat acagaacgca tgaaattcta
    14701 tccaatactt gatgtgtttt ttaatttata tgtaagcaag acaactattt ttatgcgatt
    14761 ggaacacgcc aatcaaggca tgtttaagca aaaaggaatt tatacttcgc ccaattatgg
    14821 ctatttagat agaacctttc gagttggtgt aaattggcaa ttttatgatt aagcaacgta
    14881 aaaaaatatt attcctaacg ccacaattac cttatcctgt gcatagtggt ggatttataa
    14941 agtcatacag aatgttggtt catttggctg ctcaacatga tgtagatttt gcatctttat
    15001 taaaaggcga agatgttcaa ttgttagagg aatttaaagc aagtattccg aatataatgc
    15061 aattttttac tgtaccatca aatattgaaa gaaatgctat aaatattttg aaagccaatc
    15121 tgcaatttaa acctatctct ttttatagaa attttagtaa gcaattggac aaattgcttc
    15181 aaactatttt tgctaattac gacatcatta tagtcgatca tgttgatatg tttcaataca
    15241 ttccaaagca atattttcat aaaacgattt tgcatcaaca caatgcagaa tatgtgatgt
    15301 ggaaacgtgc agctgaagta gaaagtaatt ttatcaagaa aatagtattg tttgtacagt
    15361 tctatttgtt aggaaaatta gaacatactt attgtaatca agcaaaatat attttagcat
    15421 cgccaaatga tgaagtcgaa ctcaaaagaa taggtgttcg caataataat ttctttgaaa
    15481 cataccattt aggcgatgat agtttattac atcaaactcc attagttttt gacaaagaaa
    15541 ataagaattt gttatatata ggcacactta cttgggaagc aaatattgat ggcttaattt
    15601 ggtttttgga taatgtttgg caaactttaa gtaatgaaaa taaaggaatt tatttaacca
    15661 ttgtaggcaa aaatccagac aagcgattac taaaatttaa acaacaattt gacaatataa
    15721 tttttcctgg atttattgag aatgtagagc cttattttca aaatgccaaa ttatttattg
    15781 tgcctttgcg ttttggaagc ggcataaaag taaaagtaat ttctgcaatg tatcgtggga
    15841 taccttgtgt tacttcagat attggggctg aaggcttaat ggtagaaaac tgtaaacaaa
    15901 ttatgctagc taatgatgtg aaatcatata taaatgctat tcaaacgctt ttaaatgatg
    15961 aagataaatg gaacacaatt tctaaagaaa gtcggatatt agctcaggaa aaatacagtt
    16021 gggaagctgt ttttaaagtg atagatgaag tgattacaaa atttccataa ttatataaca
    16081 taaaatatac tttttttatt cgcttttttg tgatgtaatc aatacattta ctcatcgaat
    16141 tttgaaattc ttcaaatatt caaagtattt tttaatcatt taatttaatt attatggcac
    16201 ttagattagg cgacatcgca ccaaatttta cagcagaaac cacagaaggg aatattgatt
    16261 tccacaattg gatgggcgac aaatgggctg tatttttttc acacccagca gatttcactc
    16321 cagtttgtac aactgaattg ggttatacat caaagttaaa atcggagttt gagaaaagga
    16381 atgttaaagc aattgcatta agcgtagatg acttagcttc tcacaatggt tggattggcg
    16441 atattgagga aactcaaggc gtgaaacaaa attttccaat cgtagcggat gaaaaaagaa
    16501 cagtagctga attgtatgat atgattcacc caaatgaaac aaataaattt acggttcgtt
    16561 ctgttttcat tattgacaat aacaaaaaaa tcagaactat catcacttat ccaccttcca
    16621 ctggtagaaa ttttgatgag attttaagag tgatagattc attacaatta acagacaatt
    16681 acaatgtagc cacaccagta aattggaaag aaggcgaaga tgtagttatc gctccaaata
    16741 tctcggatga agatgcaaaa gtgaagtttc caaaaggctt caaaacactc aaaccttatt
    16801 tgagattaac tcctcaacct aataaataga tttttaattg aatttatttt taagttggat
    16861 aataaataat tctttccttt agttccatat ttagatgcct aagattttaa gtatagatta
    16921 tggaactaaa aggacaggat ttgctatcag cgatgatagt aagatatttg catttggatt
    16981 gagtacccaa gaaacgtcaa acaatttaaa gtatatccaa aatataatta caaaagaaaa
    17041 tgtcgataca attttaatcg gcattcctaa aagattgaat aatgaattgg ctgatattgt
    17101 tccaaaaatt gaagcattta ttgataaaat tcaacaacaa catccacaaa tcataataga
    17161 aagattagat gaacgtttta cttcaaaaat ggcatttcag tctatgatag atagtggtat
    17221 gaagaagaaa gatagacgta acaaagcctt ggtagatgag atttctgcta ctatattact
    17281 ccaaaattat ttggaatata aaaactaaaa caataatcgt atttttgcaa tatgatttta
    17341 cctattatcg tttatggaag tccaatttta aagcaaaaag catctgaaat tgatgctgaa
    17401 tatgaaaatc ttgctgaatt aattcaaaat atgtggcaaa caatgtatca agcaagtggc
    17461 attggtttag cagctcctca aatcaataaa tctattcgat tatttatcgt agatacagct
    17521 caattggaag atagaaaaaa agatgaaccc aattttgtgg gtattaaaaa agtatttata
    17581 aatccacaaa ttacagaaga aattggagca gaatggaaat atgaagaagg ttgtttaagt
    17641 attcctggta ttagagaaaa tgtggaaagg aagcccgata taacaattac ttatcaagat
    17701 gaacaattta atacaatgac tgaaagttat tcgggattaa atgctcgtgt aattcaacac
    17761 gaatacgatc atattgaagg cgttttattt acagataaag taaagccttt aaccaaaaaa
    17821 ttaattcaat ctaaactcaa taaaataatg cgtggagatt ttagagctga ttataaaaca
    17881 aagtctgtta aataagtttc tactctttaa ataaatggat acattttgta tcttacctat
    17941 atgacaatac gctggctatt taataattta atcaatgctt acgtttattc aacaataatt
    18001 gttttagtat ttacttctat aataaatatt ccaaatttta aattagaaag ttattttaat
    18061 aaaatcaatt atagcatttt aattattgtt tttgccaacc tactttttgt atttagtcaa
    18121 acgccttttt tggttcgtga atataaaata tttcccgacc caactgaaca taaaatatgg
    18181 attaaggatt tttgtaatta tgcctattta ttattgacaa tatttttagg atttatattt
    18241 cagcttgttt ttttatttaa gaaaaataga atgaatctaa cttgtactat aatatcagtt
    18301 ttgcttttat ttatactaat tcatttaggt agtatagtag tatttggttt tcgaattaat
    18361 tatgaattac tatctgcgac gacaacttat tttattaata tttccaacct aatatatata
    18421 gcaatatttt caattgcctt ttttattttt cacagattta taatcaaaaa ctaaaatgaa
    18481 tgatattaac caacatattc aactaattaa aagtagaata gaaagtgctt gccacaaagc
    18541 aaagagaaac tcaagtgaag ttcgattatt attagccact aaaacagtaa gtcctgaaaa
    18601 tattaaaata gccattgcat ctggcgaaac tttgattggt gaaaataaaa tacaagagct
    18661 aaaagaaaag tttgaagatt tacaacgcat tccacacgaa aaacatttta ttggacattt
    18721 gcaaacaaat aaaataaaag atattttgaa atatggcgta acttgtgtgc aatctttaga
    18781 tagaatagaa ttagctgaaa aattacatca aaggttaagt gtagaaaaaa aacaactaga
    18841 tgtttttata caagtgaata cttctgcaga agaaagtaaa tttggtgtta ctccagaaca
    18901 agcgatagaa ttgataaaaa gcgtagcaaa atttgatacc ttaaaaatta aaggcttaat
    18961 gacgataggt ttgttcagtg ccgaaacaga aaaagtaaga ccttgtttta aaatacttaa
    19021 aaatttacag caagaaatta ttgctcagaa tattccaaat gttgatgttc aagagctttc
    19081 tatgggaatg agtggcgatt tagaaacagc tattgaagaa ggagcaacca ttgtgcgagt
    19141 tggtaccgca atttttggac agcgaattta tcctgatagt tattattgga atgaacaagc
    19201 ctaaattaag aagtaaattt ttgtagatga ttaaatgttc taatattctt tttagatttt
    19261 aaatgtgcta cttaatataa atttaacatc aaaaattata ttgattatca gttgtttata
    19321 tttaacttta ttgtaaatga aattaggaat agtttgaaaa ttaaaataaa attgtacctt
    19381 tgcaactcca aattaaaacg ttttttaaaa tagaatatat accaaaatga aacgattaag
    19441 ttataaaaca agaacagttg tgccaagtga atcagacagg acttggtacg tggtagatgc
    19501 agagaataaa actctaggta gaatctcatc tgagattgct aaagttttac aaggaaaaaa
    19561 caagccatct tatacaccaa atattgataa tggtgaccac gtaattattg taaatgcaga
    19621 gaaagttgca ttgacaggaa ataaattaac acagaaagtg tacattagac acacaggtta
    19681 tcctggaggt caaagattta tgacaccaaa agaagcttta gttaaaaagc ctactttcat
    19741 cttagaaaac gcaattacac acatgttgcc aagaactaga atgggaagag taatggctaa
    19801 gaaattacac atttatgtag gtgctgaaca tccacacgct gcacaaaatc ctaaagcatt
    19861 aaatatctaa ttaaaaaata acaataaata tggcaaatac ttcaacaaat ggcttaggcc
    19921 gaagaaaaac agcggtagca agagtatttt taaagccggg tacaggtgtt attaccatca
    19981 attcaaagcc ggcagaccaa tatgtttctc taaaatatct tttggataga gcagtaaaac
    20041 cattacatgt ggttgctcag gaaaataatt ttgatgtaaa tatcaatgtg aaaggtggtg
    20101 gcattaaagg tcaaactgag gcaatctctt taggtatcgc tagagcatta atcaaaattg
    20161 atgaagctaa taaaccagca ttaaaagctg aaaaattaac tacaagagac cctagaatgg
    20221 tagaacgtaa gaaatttggt aagaagaaag cacgtaagag cttccaattt agcaaacgtt
    20281 aatattatta ttgattacat tttaaaaaag atacaagaat gtctaaaata tctcaacaag
    20341 aattattgga tgccggagta cacttcggac atttaaagaa aaagtggaat ccaaaaatgt
    20401 taccatatat ctttatggaa agaaaaggta tccacatcat agatttgaat aaaactcaag
    20461 aaaaattaga ggaagctgga gctgttttga aatctattgc taaatctggt agaaaagttt
    20521 tatttgtagc tacaaagaaa caagcaaaag atatcgttga agaagctgcg aagtcggtaa
    20581 atatgccgtt cgtaactgaa agatggttag gtggtatgtt gacaaacttt gctactatca
    20641 aaaaatctat caagaaaaag caaaacatcg agaagatgtt ggctgataca aatttaaaca
    20701 gtatcactaa aaaggaaaaa ttgattttaa gccgtcagca acaaaaatta gataaagttt
    20761 taggtggtat tgaaaattta ccaagagtac cagctgcttt gtttatggta gatatttcgc
    20821 acgaacattt agcattagcc gaagcaaaaa aattaggtgt taaaactttg ggtatggtag
    20881 ataccaactc tgatccaaat ttagttgatt ttgcaattcc tgctaatgat gatgcaacaa
    20941 agtctatcca aattatcgta aattacttaa cagcacaaat taaagaaggt ttggctgaac
    21001 gtaagaaaga taaagaagaa actgaatcaa caggagaagt agctatcacg aaagaataaa
    21061 tcatacagcg ttttgtatgg tgattatcaa tcattcaatt taaaattaag atgtcagtaa
    21121 caataacagc agcagacgta aataaattaa gacaagaaac aggagctggt ttaatggatt
    21181 gcaaaaaagc tttagtagaa actaatggcg attttgaagc agctatcgat tatttaagaa
    21241 aaaaaggtgc aaaaattgca gcgaatagag cagaaagaga agctaatgaa ggtgtggtgg
    21301 tagccttgac ttcagaagat ggaaaatttg gagttgctgt aaatctttgt tcagaaacag
    21361 atttcgtagc aaaaaacgaa gattttatca atttagctaa aaatattact caaattgctt
    21421 tagataatag agtaacttct gctgaagcat tagaggcttg tactattgat ggcgtaacaa
    21481 ttaaagataa attgttagac acagttgcaa aaatcggaga gaaaatcgaa gtgaaaaaat
    21541 ttgattttat tcaaggagaa ggagttgttg cttatataca catgggtttc agaatgggtg
    21601 ttttggttga attaaacaaa gctatcaatg aaagcaatgc tgttgtgggt aaagatgtag
    21661 cgatgcaaat tgctgcaatg aatcctgtgg cagttgatgc ttctggagtt tctgaagaaa
    21721 tgaaagaaag agaaagagct attgctaaag aaaaagctat cgaagcaggt aagccagaaa
    21781 atatcatcga aaaaattgcg gataatgccg tgaattcaat gattaaggaa aatactttgg
    21841 taaatatgcc tttcgttaaa gactctagta aaacagttga gcaatatgta aaatctgaag
    21901 acaaagattt acaggtgctt tcgtttagaa gggttacttt atcataaatt aaaaaagaga
    21961 actttaaaag ttctcttttt ttttgtttac ttttacagcg tgataagaat attttatact
    22021 ttattatttg ttgttttact ttcattgtta ttgcctgttg catgtacaaa gaagaagtct
    22081 gaatgtgctg attgttttgg cattgtagag gcttttaata gtgaaataat aaaatggaat
    22141 tttgattctg taaaatacag aaaccccaac gccattgaat ctcaatggtg tgaaattcta
    22201 gatcaaatag atggaaagcc tgttcaagat aaaaatggaa atacgatagg tacatttaga
    22261 aaagaatgtc ctacaattac tacaccttaa aatatatcaa agctaaaaat aatatacaat
    22321 catcatgtca aaaccaatta aaagaatttt acttaaactg agcggagaag ccttaatggg
    22381 cgatcaaaat tttggaatac aagcacaaac cttagatagt tttgctcaag aaattgctga
    22441 agtgcttgaa cacggttatg aagtaggaat tgtcattgga ggaggaaata tttttagagg
    22501 attacaggct gggaatattg gaattgagcg ttctcaaggc gattatatgg gtatgctggc
    22561 aacagtaatt aacggaatgg ctttacaagg agcgttagaa tctaaaggtg tttatacacg
    22621 attgatgtct gcattgaata ttcaacaagt ttgtgaacct tatattagaa gacgtgctgt
    22681 aagacattta gaaaagggga gatgtattat tatggcagca ggtactggaa atccattttt
    22741 tacaacagat accgcagcgg cacttcgtgc gaatgaaatt gaagctgatg taattctaaa
    22801 aggtacaagt gtagatggtg tttattccgc agaccctaaa aaagatagtt cagctacaaa
    22861 atatgaaact attggtttta atgaagtctt agtaaaaaat ctaagaatta tggatcaaac
    22921 agcttttgct ttgtgtagag aaaataatat gcctattgtt gtttttaata tccgagagaa
    22981 aggtaatatt ctgaggattt tacaaggcga aaaaatcgga actttgattt cttaaataaa
    23041 aaaagtgcgt tagattttta acgcactttt ttattattgg atgttttcat ttttgaaaac
    23101 tattatgcat acatttcttg tcttaatgct tgaattttct catcagctaa ataatcatcg
    23161 taagacatta atctgtcaat aacgcctttt ggtgtgattt ctattattct atttgcaatg
    23221 gtatgtgtga atgtatggtc atgtgatgtg aaaatcatcg tacccgcata gtcaattaaa
    23281 ccattgttta atgctgtaat actttccaaa tctaagtgat tggtaggttc atctaagata
    23341 aggaaattac cttcttctaa cattgttttt gaaagcatac atcttacttt ttctcctcca
    23401 gaaagtacag aacatttttt aagcgtttct tctccactga ataacattct acctaaaaaa
    23461 ccacgtacaa aggtttcgtc cttttctgtt gaatattgtc gcaaccagtc aattaaattc
    23521 aaatcaactt caaaataaga tttattgtca tttggaatat aagatttagt aatggttaca
    23581 ccatattcaa aggaaccact attggcttgt gtttctccag ctaatattct taaaaatgtt
    23641 gttacagcca tactgttatc tgaaatgata gcaactttat cattcttgtt aattgtaaat
    23701 gttacattac tgaagaaagt tttaccatcc tcattcttgt aagctaaatt atgaacatca
    23761 agaattttat ctccagcttc tctagtttgt ttaaagaaaa tgcccggata ttttctgctt
    23821 gatggtttaa tttgttctat atctaatttt tccaatgctt tcttacgaga agttgcttgt
    23881 tttgatttag aagcgtttgc actaaatcga gctataaagt ctaataattc agctttttta
    23941 gcttcatttt tcttattctt atcggctctt tgttttaata ataattgtga agtttcgtac
    24001 caaaaagtat aatttccagt aaataaattc actttagaaa agtcaatatc aacaatatgc
    24061 gtacaaacca tatctaaaaa gtgtctgtcg tgtgatacaa caattactgt gtttttaaaa
    24121 tctgctaaga agttttctaa ccaagtaata gtttttaaat ccaaatcgtt ggttggctca
    24181 tctagaatta aaatatcagg attgccaaat aaagcttgtg ctaataatac ttttattttt
    24241 tgatttgaag gtaattcttt cattagtttg tagtgattat cttccactac atcaagatta
    24301 ctcaataatt cagcggcatc actttctgca ttccaaccat ccatttcagc aaattcagct
    24361 tctaattcgc caactttcaa gccatcttca tctgtaaaat ctgctttagc ataaatagca
    24421 tctttttcct tcataatgct atacagtttt tggttgccca tcatgactac atcaatcact
    24481 tgttcttcgt caaactcaaa gtgattttgc tttagaatag acattctttc accaggagaa
    24541 atttcaattt gtccagttgt tgggtcaata tcaccagaaa gaattttcaa aaaggtagat
    24601 tttcctgcac catttgcacc aataacacca tagcaatttc caggtgtaaa ttttatattt
    24661 acctcatcaa ataatacacg cttaccaaac tgtaatgata agttttgaac agttaccata
    24721 cggaatttat ttttttaaat tataatagat tttggtttat caaccaatgg tgcaaagata
    24781 caaattttaa agaggattct actttactaa aagtaaaatt aatatcacga ttccagcaaa
    24841 aatacgataa tagccaaaaa acttaaatcc atattttttt attacttcta caaatgtttt
    24901 aatagctaaa agagctacta gaaatgcaat aatgtttcca attataaaaa tagataagtt
    24961 tccagattgt gctatcattt catagccttt ttgaacattt tcgccagttc cccaatcttt
    25021 taaaaataca gaatataaag ttactgcagc cattgtagga actgctaaaa agaatgaaaa
    25081 ttcggcagca acatctcgtg ttagtttttg ttgcatacct ccaataattg atgcagcaga
    25141 acggcttaca ccaggcatca tggctaaaca ttgccaaaaa ccgatgctta tggctttttt
    25201 tacagttatg tcttcttctt tttctattat cggatttttg aaaacattat cgataaataa
    25261 taagacaaca ccgcccaaaa tcattgcgat tgcaatagga attggactgc ctaaaagttc
    25321 atctatttta tcatcaaata gtttgccaag caccaaagct ggtacaactg caagtgctaa
    25381 tttgatataa aaatcaaaag atttgaaatt aaaaaatttt ctccaataaa gtaccacaac
    25441 tgaaagaata gctccaaact gtatagaaac ttgaaacatc tttacaaatt catcttcttg
    25501 aataccaaaa aaagtacttg taaatatcat gtgtgctgta gaagaaatag gtaaaaattc
    25561 tgtcaatcct tctacaattg caataataat tgcctgaaaa atatccataa gattttattc
    25621 tgtaatttga gtttttggtt tttttagtat agcaactgca ataactgcaa aacctaataa
    25681 tatagtaatt ggagcaaggg taattcttct aaaagaatat aattctgctt cattaaatac
    25741 tttagggtct tgattgccac cactcataaa aataaatcct agtattatca aaatgccacc
    25801 aatgccaatt agtatatagt ttattttatc aaataaaata gtggtatttg tgctttgtgg
    25861 tattttaaaa aatgaaccag aattgctact ttttgatata ggctttttcg gttgtgaaat
    25921 tggagcaacc ttaggttttg gattgttttg taatggtttg ttttttgcca taaaatatat
    25981 tttaaaataa atcgtctaat tttatattcc agtatttatt gactgcaaag aaagtactaa
    26041 ttaatgaaat tagcaaactc gttagtaata agccgccgcc aattccagct aatactaagt
    26101 aatccatttg tactaattgt atgctgtatt tgtcagtgaa gaaaaataca atacctacta
    26161 atattagtat agctataaat gtactgatta atgcagcaga aatggctttg attaaaaatg
    26221 gtttgatgat aaaccacttt gttcctccaa ttaattgcat agttcttata gcatatcttt
    26281 gtgtgtacat cattaatcgg atggtgctat ctattactga aaatgcaata atgaataata
    26341 tcaatgaaat ggctaaaatt ccaataaaca aaggtcttac agtgttgcca aacatatcgc
    26401 taataggttc agaataattc acttctttga ttccatttat agataaaaag tcagatttta
    26461 tagctttaat ggaagattta tctgcttttt cagcttttaa atttacaatg aatgcattat
    26521 acattggatt gtagcctaaa attttagtaa aattttgtcc taattctttt tcatacattt
    26581 ttgctgcgac ttctttgctt ctgaaattga ttttatgtaa ataatctttc ttttgaagtg
    26641 ttgctttaat tgcgtcaata tttgaatttt ggaggctatc atctaagatt aattcaattt
    26701 caaaactttg tttaagtttg atgagtgaag catttgaata ataaataata atagcaaata
    26761 atccaataat aaataagtta agtcctatag ttaaaatagc atatttgtaa gtaggttggt
    26821 atcttttttt gggtttatat ttttgttctt ccgccataaa ctaagcgaaa gtaagcaatt
    26881 tatttttttt attaaaaaat ttaataaaca ttaggatatt ttctgtctct atattttaaa
    26941 aatggcgatt ctatgtattt gtaaattagc tgagcaatac cataatttat tagtaaaaat
    27001 agtaagatat acattatgct actgccaata ctgtcaggta ttaagttgtt tctgaagaaa
    27061 aataatttgt agataggtag atttatcaaa tacatactat aagccaattg acttgatttt
    27121 ttaaaaaatg cacttaggta tttttgttta tctaagttta aataatatag ctttggaatg
    27181 attagtgccg taataaatcc gagtgttggt aaaaatataa aattacaata gatagcgata
    27241 aggttgttgt gatgatatag gtaaaattta ttctggtagg cagcataaaa aagcggaact
    27301 gtgggaatta ttaataataa tccaaaccaa aataaccaat ttttatactt taaaaatagg
    27361 gtattgtaat agtaaaaaca aacataacat agaattccaa taattagatt atcaaatctt
    27421 aataatacta cttttctaat attttcatca aaattaagat gtattccgta ttgttgaata
    27481 tgaaatattt tcaatgcaaa gctgaataaa aacatcaata agcaagtaag caatattgag
    27541 tttttaaagt ctaatttttt gaaattataa atagcaatta aaattatagg taatagacaa
    27601 taaaaccatt cttcggtggc caaactccaa gcttctctat aaaaatcagg gtgcggataa
    27661 tttaagtttt gtatgaataa aatgaatttc caatcgaatg ttttttcttt aagtatccaa
    27721 acatataaaa ttgtatttac gatgtacata aaataataat tgggcaatgt ccttaaccat
    27781 cttcggatta agaataattt caattgagca aaattattac gattattatt aaagtatttg
    27841 aaaacgatgt ccgtaattaa aaagccactt aatacaaaaa atatttcaac tcctgaaaat
    27901 ccaataattt cgtgaatagc taaagccttg ccagctaagt ttccaataat tggtaaatga
    27961 attaatggat taaataatgt cgcattatga ccaaataaca ccataaataa tgcaaaactc
    28021 cgtaaagcat ctagtccaat tatcctttta ttgattgtaa tcaaattatt cttaatcttt
    28081 gtgctaattt aatgcattca attatatttg tattatgaaa aatgcacctt tcggaattga
    28141 attgtattta tatccaactt attttataga ttgtaatcat cctgatgtaa tagaatatgc
    28201 tcataatatc atcggagatg aaaaggataa aaaacaacaa gcgataaagt tatacaatgc
    28261 aattagagat ggatttcgct ataatcctta taatatcaat ttaaaacatg aagaattaaa
    28321 ggcgagtaca ttgctcaata gagcttcaaa aagtggctat tgtacggaga aagcttgttt
    28381 gttggctgcg tgtttacgtg tggtgggcat tccgagtagg atgtgttttt ttgatgttag
    28441 aaatcatatt ggcgtggata aatatattga aatcattaaa actgatgtgc ttactttcca
    28501 tgcttgtaca gatgtttttt tagataataa atgggtaaaa gccactccag ctttcaataa
    28561 aacattatgc gataagctag gtgtcgctgc attagaattt gatggcgaaa atgattctat
    28621 ttttcaagaa tttgactcca aaggcaacga atttatggag tatttacacg attatggcac
    28681 ttttcatgat ataccacacg atatgtttgt tgctcaaatg aaaaagcatt acggtcattt
    28741 ctttgagaat ttagaggcaa caaaagctat ggtaaatgat agtgaataac ttattttagt
    28801 tcgaggatat agtcaataat acgcttttct ttttgaaaag aaaaaacttg atttagtcct
    28861 aaatattcaa tagcatcttc tattttagct tggtctgata ttttattaga aggattttga
    28921 acaagtaatg ctttttgagg aattagacca atcaattcac tgccatttac tcttattccg
    28981 aaatcattag ctatttcttt tacagtacca aaaatataaa aaataggaga ggcgttatag
    29041 tttgtaatat tagtagaaat ttgtacaaat ccatattctt cgatatacca agcaatagcc
    29101 ctacaatatt gcaattgagg atatttttcg ttatctcttt tgtctcgaat agcttttgca
    29161 atttttttgc ctatacttaa atctttggtt tttaaattga tattataagc taataaaagt
    29221 gctcttgcac ccaatgccat cataccaaat ttttgattaa aaacagttgg tccaaaatct
    29281 ggtttccatt ctttttgtat tattttatct gcaattgctt catattctcc ttttcgtata
    29341 tcggctaaat tttttcgtgc ttcagttgtg gctgaatttt catataaata gacaggtaag
    29401 cctaaatcgc ctagttgttt gcctaatttt tccacataag ttttagttgt ttccatatcg
    29461 atgttttgat agggaataag cggacaaaca tcacaagcac ccattcgtgg atgttttcca
    29521 gcatgcaatc gcatatcaat gagtttatat gcttttttga tagctttgta agttgcttct
    29581 actattgctt caggctttcc aatataagta taaactgttc tattagcatc gaaaccaata
    29641 tcaatatgta acaattgaac agaaggtgtt tgtcgaattt cttcagcaat agcgtcaatt
    29701 gtagctttat ttcttccttc tgaaaaatta ggtacacatt caattaaaat atcggaatga
    29761 ctaaacatta ttacaaatta tcaattataa attaaattac aaaatccata ttgtattgtt
    29821 ttttaaattc taaagcatag gagaatttaa gacactaaca atctattcat aaatatttct
    29881 ttaatgatga tactgagtcc taaaattaat agcacccaac caaaaaatgg ctttaatttt
    29941 ctgccatcca ttcttcgaga taaaaaagtg ccaattagta cacctgaaat agtgcaactt
    30001 actaccgaaa ttagaagttt ccaatccata cctgcaatat ttggttcgcc taaaaaaccg
    30061 attaatgatt tggctgcaat aattaataat gtcgtaccaa cagcttcttt cataggtaat
    30121 ttaagtagaa taaccaatgt cggaacgatt aggaatccac caccagtacc cactaatcct
    30181 gtaataattc ccaccactaa cccttctatg gaaattaatg tgtaatttat atttaatttt
    30241 ttgcgttctt ttacttcatt atcgttggac ttctttatca tactataaga agcaattgcc
    30301 atcagtattg caaagaaagt aatgataaat aaatcttttg taacggtaaa tccattaatc
    30361 gttattattt tttctggaat attggggata atataagctc tagtgaaaag tacgcctaat
    30421 atagatggaa taccaaaaga tataaccgtt ttaacatgaa taagcttatt tctaatataa
    30481 gaaattgaa
//
LOCUS       DAOQCW010000014        30435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_553_length_30435_cov_5.386932, whole genome shotgun sequence.
ACCESSION   DAOQCW010000014 DAOQCW010000000
VERSION     DAOQCW010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 30435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30435
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_553_length_30435_cov_5.386932"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="PLS16_02410"
     CDS             <1..423
                     /locus_tag="PLS16_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59959.1"
                     /translation="QGSSQKINGLNAYHFIAQYGEQQQSQEAAQILTVHTTLIEYNSN
                     IYIFLGLSDSKDYNAYKSNFTYTTNGFKQLTDVSKMNVLPERVKVITVNNTSSLSQVL
                     SQHNIASNRYEELAILNGMETTTQIPAGTLIKIIEKRK"
     gene            437..757
                     /locus_tag="PLS16_02415"
     CDS             437..757
                     /locus_tag="PLS16_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNJ59960.1"
                     /translation="MAVQDLSPKEFLAAYNNTTGEKELIDVRNPHEIEEISIEGHVAI
                     NIQHPSFVEKINALDKNKTYFVYCRSGNRSGQACKYMASQGFENLYNMKGGMLEYVET
                     DFDD"
     gene            complement(758..2209)
                     /locus_tag="PLS16_02420"
     CDS             complement(758..2209)
                     /locus_tag="PLS16_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNJ59961.1"
                     /translation="MFKKLPETGISKDIIISELTELRNKDADWRKGRVFSLVFYPGDD
                     AANVVQEAYKLFSSENGLNPTVFKSLKQMESDVLSFTASLLNAPESAVGTMTSGGTES
                     IICAVNAAKKYAFSKNNNITRGNIVMPESAHPAFNKAADYFNLEVRTVKCIDGLEPDM
                     AQYISKIDYNTVMIVGSAPAYPHGHIDPLEKLNQLALERNLWFHVDACVGGFMIPFLE
                     QLGYNVPVFDFRMEGVSSISLDIHKYGYGPKGASVVYYRNAEKRKKQFFVYTKWIGGL
                     YVSPSISGTRPGGAIAGSWAIMNYLGSSGYKKMAEETYQAVQKMKNAVNAIDGIEVTG
                     NPLTTIFSIKSSGKLDIYRLGDELTELGWHLDRQLTPATLHVTANYVHVAYADEFISD
                     LKKAVEQLQTTSLENIGDALKQKIVQQGAKILPENWIKKLSKKSIEDVSKTESSNTKT
                     APLYGLIGELSGSGTLDEMVLDLLDNMNKPSQE"
     gene            complement(2291..3619)
                     /locus_tag="PLS16_02425"
     CDS             complement(2291..3619)
                     /locus_tag="PLS16_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNJ59962.1"
                     /translation="MTTHQLTKQIGLLTGVAFVVGSVIGMGAFVLIPLICAKAYGAAW
                     LSITIALVVCMLNVFPIIQLSAAYPVAGGGYEYGNTFFNKTTGVVFSSWALVGGAASV
                     ALVAYGLVESFQTVFQIPFSNHLLSLIIMVLFWGVFLTGVKTIAAMQVVLVVQTLLAL
                     LLYAIPTLANNWDQVVFSLPSEGGSFFMSIIFAFNISLGFQIIMELGEEMENPKRNIP
                     LSLFIGGGIVWLIYILVTAAYIGVVGIENITTKPEMVNTASSILPNWALILIRVGIIS
                     AGLTCFIGSGMGIPREVFALARAKIIPEKYAEVSTNGTPKYAVNIFFTIVCSVFLIGE
                     LFQRIGIMDYFFGKDVIEFYSYITIFGIMALSIGISLASLKLKKMMPDAYENAYIKFH
                     PTSLIVLVIIAVIASLFLIVLVSMKWVVPLLFILITLFVIIWYKQKSIVL"
     gene            complement(3749..4321)
                     /locus_tag="PLS16_02430"
     CDS             complement(3749..4321)
                     /locus_tag="PLS16_02430"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNJ59963.1"
                     /translation="MKIFNYIFLFTLLFTSCNSNSKSKKEISESSIFNLTSEWENQDA
                     EKLQLKDLQGKTLVVVMIFTTCKSACPILVAKMRGIEEKISRKDIDKVSLVLVSIDPE
                     TDTPENLKKFAKKNKMDSPQWIFLRSSEAATQEFANVLSMKYKRISPIDFSHSNIISV
                     FNDGGDLVSQEEGNIQIDDVVATVNKTIKK"
     gene            complement(4323..5177)
                     /locus_tag="PLS16_02435"
     CDS             complement(4323..5177)
                     /locus_tag="PLS16_02435"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNJ59964.1"
                     /translation="MEVNRFFILLIALLVVSCNPKGNVNTEELTSQENLVDTLVKIQT
                     DVKMVYIPGGEYKPFYGSEKVNVTVAPFLIDERPVTNQEFLEFVIANPQWRKSNIKKV
                     YSDSSYLRDWVSDTELPKGANPDAPICFVSWFAAKAFAESVGKRMPTLDEWEFVAMAD
                     AEVPNAREKKSYSDNIVDLYLQKDRQFNAVKQSPPNYYGVYNVFDLIWEWTDDFNSIM
                     TTGDSRNGEFNDKNLFCAGGATAATDVLNYAAFMRFALRTSIKANFTIANLGFRCAKD
                     TIVETDIK"
     gene            complement(5197..6252)
                     /gene="nirK"
                     /locus_tag="PLS16_02440"
     CDS             complement(5197..6252)
                     /gene="nirK"
                     /locus_tag="PLS16_02440"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HNJ59965.1"
                     /translation="MRTKHFLNLCAIALFAFALSACKTTPSSSGSGSDVAVDMVASGD
                     PEVAEVTGAPNVPAPVGDRGPKKWIVNLETTEIEGFIADSIPYTFWTFNNTVPGSFIR
                     IRVGDEVTLNLKNAANSVLPHNIDLHAVTGPGGGAAATTAAPGQEASFTFKAINAGLY
                     VYHCAAPPVPMHIGNGMYGLILVEPAGGLPKVDKEFYIMQGDFYTKASAVKKGLLEFD
                     NDKAVAENPDYVLFNGAKGALLGANMIEAKVGETVRLFVGNGGPNLTSSFHVIGEIFD
                     NVYLEGGTTISHNIQTTMIPAGGAAIVEFKCEVPGEFVIVDHSLSRAFNKGAIGKLKV
                     TGDANPKVFNAKTLVKE"
     gene            complement(6273..7631)
                     /locus_tag="PLS16_02445"
     CDS             complement(6273..7631)
                     /locus_tag="PLS16_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59966.1"
                     /translation="MKAFNNLYNTSKLLSFIVLLLVVNFSNAEEQDSVKNNTFSMSGQ
                     FRPRFELRDGAFRPLLKNEKPAALVSDRIRLTFDYAYKNVFSLRITPQAVSVWGQATM
                     TQGAEDKGSKFSIFETWAQVYASPEWSFKLGRQVISLDDERFFGELDWAQGGRVHDAL
                     SILYKKEKYELRGYFAFNQNYRALYNNNLSNPAGSLFSTNNALPYKWMQTVWASVPIK
                     EHSKITFLVSNLGFQNAITTTKDTVTNYAQTFGTNYNYNGSKLNVSVAAYYQMGKDLT
                     NIKNQGYMLSAALVYNINKKWNIGIGSDMVSGNNVGATQKKSHIFTPYFHTGHKFYGF
                     MDYFYSGNPHKNAGLSDTYLKINFKTEKGLGLNMAFHQFVTPNKLIDGATKFSKNLGQ
                     ELDLGISYKIHKFVGLTGGYSFYLANSSINFLKNTPNAKVYQQWMWLGLNITPTFFKT
                     NF"
     gene            complement(7890..8300)
                     /locus_tag="PLS16_02450"
     CDS             complement(7890..8300)
                     /locus_tag="PLS16_02450"
                     /inference="COORDINATES: protein motif:HMM:NF025589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HNJ59967.1"
                     /translation="MANYKIEEIEGIGSVLGKQLRDTGINNTDKLLENSKTPKQRKQL
                     AEASGIAEKLILRFANMADLFRISGVGQEYSELLEKSGVDTVAELAQRKADNLTKKME
                     TVNAEKNLTRRTPSLKEVEKWIEQAKTLPRALEY"
     gene            complement(8410..11691)
                     /locus_tag="PLS16_02455"
     CDS             complement(8410..11691)
                     /locus_tag="PLS16_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNJ59968.1"
                     /translation="MSEVQIKQMQELLKKSLIKIKQLEQEIESRQSLIKNEIISSEIP
                     KNTDVAVVGYAFRFANGIDTLQKFWNLLYEKKDAVSKIPIARFDVDEIYSEDQYEAGK
                     TNTKFGTFLEQDIALFDTDFFGIPPREAKSIDPIQRILLELTYEAFENAGIPSNQLKT
                     KNVGVFVAVGLSDYAQARLRSGNLEDIDVYDATGIPFATICGRISYSFDFVGQSISVD
                     TACSSVLVAIHQAQQALNNGEIDVAVIASANLLLTPEIFVALTKMGSVSPSGKTKAFD
                     ENADGYVRGEGASVLVLKRREDAEYNNDNIEIIIKSAAVKHNGTSNGFTAPNPQVQVD
                     TIKTALSNANLSVDSIDYVEVHGIGNRYTDAMEVQAIHQAYKNRQRPIQVGSVKSNIG
                     HLEACTGMPMLFKIIAAMQNKIIPAQIHLHELNKDVDWQKIKVNITKENTDWLKFDGS
                     KRLAAINLSGYSGTNTHLILEEADIKQKSEEVNAPFIFNLSAKTPDALKDVVIKYLAD
                     TSVFDKHTLSEICYTLQQRNLFEHRLSIFVKDKNDIIQALQDFIDSKQNPNLFTSKNI
                     DNNGQIVFQFTGQGSQYFGMCRNYYKSFEYFKNTIDFCDEELKQYLGESIKNVMWKHE
                     DKHLIHQTKYTQPAIFIVEYALAQLLMKYGIQPTMLVGHSIGELVALTISGVLEIKDA
                     LKIVSMRGNLMQNIPDNNGGMAAVFANAQTIVDLNNDNIVEIAGYNTTNNTTITGDKE
                     QIAIFIERLKEQNIRAVPLQVSHAFHSKDMESIFVDFQNICNEINFSFAQIPLASNID
                     GKILDKQSIDSFYLTQQLRVPVDYLKSIQNIQNTIEAEFYIECGANPVLSTLGKNILS
                     DKNISWLYTTKPQTDDVQQFYNILQTLFSNGFNIDWSLLYIGKRINRVKLPTYAWQWK
                     SYWYNPNRIGDVEPKLRNQDNKSIKQESIAKPKYASKNIVQPQVKRETLLVIMQIEAA
                     KILGLEAGESLDIQKPYREQGFDSMMSGEFLAKLESLIGNGEIKMDVIHNYPTPKELH
                     QYLIDTYFGGGAVNTNEAISMADLMFGSDISREEAGDWHTEKADDSKLLRWFKRLDNK
                     LPKAK"
     gene            complement(11695..16188)
                     /locus_tag="PLS16_02460"
     CDS             complement(11695..16188)
                     /locus_tag="PLS16_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNJ59969.1"
                     /translation="MTDTEKYSEQIKQALSAIKNLKAQLTIERNKHSEPIAIIGMAMH
                     LPGNVKNADDLWKLLINNIDAIEDIPQSRFDNSKIYDEQGGVGKNILKQGGYLKDIEV
                     FDAPFFDLTRIETESLDPQQRLLLELTQEALENSGINTAELVDSETGVFVGITSIDYQ
                     KKHFRCGDYNLVNPYSYTGSAVCANAGRISYTFGFQGPSVSIDTACSSSLVATHLAVQ
                     SLRKRESDMAIVGAANLILEPELSICFTALNGLSRDSRCRPFADNANGFVRSEGAVVI
                     VLKRLSDAEQNKDNILAVIKGSAVNQDGKSNGFTAPSVSAQTKLIQAALKDAGLNPDA
                     IDYVEAHGTGTKIGDPIELEALANVFGNNSNKKVNLASIKSNIGHTESVAGLAGLVKN
                     VLALNHQILPKNLHSDTLNSLVDWESLPVQVVQENQSNSIQNVGVSSFGVTGTNAHVV
                     VQKYDAVLKEHNAVRTDIFVLPISAKSEQALVELIKKYLQFIQEGKHTLQDICAMAAL
                     RRADFKYRKVFVAVDEQELIQQMHDTIELGVQNQTTIFDNDDFVKTIFVFPGQGAQWL
                     GMAQNLITQEPVFKASLEAFNNELSKYVSWNLFDVLNGDDEQFLNQLDVVQPILVAIG
                     IALADLWKSKDIFPNGVIGHSLGEVAAAYVAENISLHDAVKIICKRSKLMKQVSGKGL
                     MLATDLTFSEAKKRIENLQDKLSIAVVNSEQSLVLSGDSESIWHLQSTLEQEGKFARM
                     IKVDVASHSIHMDKIDEELAIALDSVQPINSNIAFYSTVYGKKIDGSSLDANYWKNNL
                     RQTVRFKDSVEQIIEQQKCVFIEMSPHPTLLHALQQNFDMQSSKSIALPSFLRDKNDF
                     ESFYKNYGALYETNYTINWKNIYPEIGKFVQLPNYAWQKERYWFDEQPNLSSTKSTTL
                     PEILNNNLPSFEIVYESINMDSIALKQQNILLLNGEGVSFLQEILEQENNVKVQQIHQ
                     SDFSKDIDIVVFNLLGTTISKLQAITILQNLVRFYDNETKRPKIILLSNGANVLDGDK
                     QLNTTASIVQGIVQSLHNEYLDIDFRTIDLPFDWKQDDIKTAIHLFNTPAQNKSIALR
                     NGLVYQPKLQIFKAKLPHKNYFFNKEQTVLIIGGTSGLGLELAKWLSTKGVNNIALVS
                     RSGLKDKTQEAIEFMKQNNTKVAVLKADFSNFVEAKTCISTIETAMPKVQTIVHAAAV
                     LGDALFQDIDRQSIQIILNSKVEIAQNIDALCQDRLQSKVIFFSSAASILGTLAQAVY
                     SGANYYLDNLAKHRMQVGLDTIAVNWGNIGTIGLAAQDTKRGANLKEQGLDLIMPNEL
                     PKYFEEFFSVEAAQFIPLKIDFTLWKKYYNNVKNNLFYTQFVEQDKTQFVEQLPQEKP
                     SILSIKENIKQHIAQITKIPSSKIKEDDTFKSIGIDSLMALQLKNKIQVDYQLNLNIA
                     SVWSHPTVEKYANFIAEELNLFEKETERIEVAEISKDNKSIEAEVDDLSLEELMKQLN
                     EKI"
     gene            complement(16185..22676)
                     /locus_tag="PLS16_02465"
     CDS             complement(16185..22676)
                     /locus_tag="PLS16_02465"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNJ59970.1"
                     /translation="MDKQISIIDDAIAIIGVGCKLPGDTSDIERLWDIVSNKIDAITD
                     IENNRFPNIDNLVNNNRANGKIVTKRGGFIKDIEYFDANFFQISPKEADKLDPQQRFL
                     LETSFNAVEDAGIPLEKLYGSKTGVFIGCWLNDFEQRLAQTLHDVDVYSTTGSGRYPL
                     SGRLSFFYNLQGPSITIDTACSSSLVAIHLAIQSLKSGECNLAFAGAANAIVDSFISK
                     GYSRSGLLSEYGACHFGSANPDGYVRSEGGAVIILKRLSDAINDGNEIYAVIPASVCN
                     NDGASDKYMLAPSAITQQIMLEEAYSKANIDVNKVKYIEAHGTGTKAGDPAEISSIWN
                     ALSKNRNGANSIFIGSVKTNIGHTEGVAGFAGLFKVLMAMKHKTIPPNLHFYEPNPDI
                     DWQNMNVVIPTELVAWETETNEPLIAGVNAFGITGTNAHVIVQSFENKSSENIVFDKK
                     YNILPISANNSAAVIDYVKLYQSQINENNYLNFTKNIAFHKANLQQRNAIVFSDFNEL
                     QLGFKAIVDKTNNEQLFSGYSTAKLPKIAFVFPGQGSQWLGMGKELYQHEIVFKDAID
                     DCESAFKNYVEWNLTDILFSEDTESLSEIDIIQPTLVAIEIALAKLWMSIGVQPNAAV
                     GHSMGEIAAAYIAGIISLDNAANIICTRSQLMKEQKGKGAMGYVALAVDELKPFLESS
                     IDNVNIGVVNSPKSTVITGDKTEVERIISTLDEQGFFSRLIKVDVASHSQQMDILKPR
                     LLDATQNIQAKESEIIFQSTVHPEIQNAKQIQADYWVDNLRNTVQFSEAIQQLIDEGI
                     EIFIEISPNPVLNSAIQENIDIKKSEAIVLFSLEKNKNDILSFTEQIAKAFCNGVFID
                     WKNFYGNQYEKIKLPPYPWQREYHWIKEIQTSSVYNQDRKVNNGLPHPFLKNYAFSQG
                     NITTHIWETNLSLEEFTYLSEHKVGDKIIFPAAGFIEIIQACCTEVNPEQQFQIEHLC
                     ILESLELSQEEAINLKITLQQAIGNFYSLKINSIKEDEVLQHLSTDIIFKPLVYKNSD
                     KVAELAQAISKETHYSACRISNLNYGNGFQGIQKIHHNESVFEAIIELPDNINIADYI
                     IHPTLLDACLQTILAPIYLKNDATYVPFSISQYKFYKAPKTSKLKSKVEVYDFDKTKL
                     VCNLWIYEADDLCVEAVGVEFRVIHQAKSEASADNLFYELESTKIPIQNSSSNDAVVL
                     LGNLKNKEIIAQAFGETRPISFIEIQSYSKAILKQIFKQIEFTPNTQLVFCVDENLEW
                     DKEDGIFSFQQRNIIGITEVVQAVVELNLEVRLWCITKGAIAVTQQEVVSPLQHQIPA
                     FVRILWNENFELKPSVIDIEKSEDLLLLPNLLNQDNNENEIAIRDGQFYATRLSHKLI
                     AQQHKSKVISKNIPFRYEMEQVGLIDNLVANAYEFSAPSANQALIEIKSVGVNFMSLL
                     SVLGICPSGKNGFCTLGIECVGIVQAIGEDVHHIQVGDVVYGMAYDTLASHTLVNANA
                     LCKVPQGYSYDELATIPAVFLTVYYSLIELARIKKGDKVLIHAATGGVGLAAIQLCKL
                     YECEIYATAGSEEKRTYLKSIGVVNVYNSRTVDFFDEILKDTDGKGVDVVLNSLVGEA
                     MYKSLQLLNNFGRFIEIGKKDIFENSKIGMEVFRKSLSYFMVDAEKLLFEKPELLGEL
                     LHEISALFEEKQLEPLPFNVFNIAQAKEAFRLINTSKHIGKIVINIEHQEDLEIHKNS
                     EFTIDEKATYLLTGGLGGLGLQFTKMLVEQGAKNIILLGRKAPSSEIESTLENYRNVG
                     VNIITYQADVSNKRHLQIVFNNIPVENPLKGVFHLAGLLDDASIINLTEDKFYNVLLP
                     KVFGALYLHELTKNIDLDYFVLFSSSAVLFASPGQSAYVAANAYLDGLAQQRKAEKLA
                     ALSIQWSTVADVGLAAVSENRSERLKAEGIEPLLAEDCTNLFQDIANIEDANIGIFKF
                     DVQKWQQNYSTAKNNPYFSLLTDSKDDKTTTVLSNYIDELKLINDKTELEHQIEQKIK
                     ETLAKVTKISIDKISTRSTFKSLGIDSLMSIQLKNQLEHIFEIKLSVTAFWTHATIKA
                     YAKFLIDKLSFNKENSTVETELRTQEFVVTSKENETPKTQDINIAQLSIDDNEPSTEN
                     QQQPTTLTHSNNEQELEDLSKLLDDELKDLL"
     gene            complement(22721..23797)
                     /locus_tag="PLS16_02470"
     CDS             complement(22721..23797)
                     /locus_tag="PLS16_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59971.1"
                     /translation="MSTVQEFNEEDVRLVDITDSIGVYFKYLLQKFYIVIIGVGGLTY
                     GLYYYAKNAEPEYLAFTSFNTVDPKGIAASGLISLASSLGFGASGTTVDLLAGLYNSR
                     LVFYNAMITDVSVDGKVEKLGDEMMRVYGLDQGFKATKGKENFGFTASDINSFSRDED
                     SIANIFYSMFIDGIVEVEYEVTSGLIFSEIVTPDYQLSKNLAGTIINKVSEYYMNEQV
                     SSASISFTTINKKIDSLKSEIDWREKKIAQDQDRSIFNVKKEGIFDQDFIRQEVSNLK
                     MMYADAIATKESAKTSMKPQASPVRVVDHPDFATTPKYKSTMLYSLIGLALSIVLVII
                     PLMLRKAVLIGREENALAEKLNSH"
     gene            complement(23801..26287)
                     /locus_tag="PLS16_02475"
     CDS             complement(23801..26287)
                     /locus_tag="PLS16_02475"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNJ59972.1"
                     /translation="MNFRNVRLLVLLIISSISISNAHAQMAGLSQLLNMGMQGYNSVL
                     NTAKTAATTKVGTTSANTAASLQQAKMMGVDPNIIKLLLGGGEDASAAVSNPNDALMS
                     SLLNPEYGQLVLQTKLRQDSMFLILDSLKVEKQKLKDIDLSNEIFGHRFFTTNNLELF
                     FKSTDTKAPDSYIVGPGDEILISVWGYADYNNKLKVNDDGYIQETEFGRLYVKGLTFG
                     AVKALIGKRLNSFINTQNTKYEITLNYSRSIDVNIVGEVVTPGTFKIPAINSVYNAIN
                     AADGVTNIGTVRDIQIRRDGKVVKRFDVHQFLMNPIPEESFYLQNGDFIYVPVAEKII
                     KIEGAVRRASLYELKKGEGLNELIAFAGGLKPNAFIKNILITRLAKDKTEIINLDYEK
                     LLASNQNFVLFDGDVVNIGYISGEIENGVSISGSVRYPGRYQLKDGYRISDLIIAAGG
                     IKLSAYTDRAYIKRVMPNQQTVITKFSLSNILLDPSSADNLLLQKNDKVELFDQSEFV
                     EKFVVTIQGSVLKPAILEYSDGLTLNDLLFYAGGLKTEAANSKIQISRVLEVNDEDSL
                     GKKFIPQRVVVQNIEIGPNLEIDEASKAFRLAPMDLVDVRKTPGFAEQMKMTISGEVV
                     YPGSYTILDKNERVLDIIERAGGLTQYAHIQSAKLIRSDMNQFKTVFELKDAFKDPNS
                     RANLIVKNGDIIEIPTINQLVRINGAVRYPNLDSAQTISGKFVAGKSARWYVKKYAGG
                     FKKEAKRKNTMVLYPNGKVEYTKSFLGIKNYPTIDVEGALVNVEQKEPKPKMPPMTRE
                     PVSLNILLPSLIAGITSALSTAMLIVFLKK"
     gene            complement(26385..27965)
                     /locus_tag="PLS16_02480"
     CDS             complement(26385..27965)
                     /locus_tag="PLS16_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNJ59973.1"
                     /translation="MGKTPNVILAFLFLLSANVVLSAEQKETSKSSIKTSSRIETNKF
                     LKDWSFTIHGGATSPFTDIRSYDWVRQFKAPSEVQWGAGLGVTKMIGNVFGINIDYTL
                     GKLSGRTVTRGGFAQDRYYWQVLGFDQEVYFKTNVFHQATANVYINWSNMFFGLNRWI
                     KSNIDQKPWKERRVSVFSKIGMGMMRLESNIYNVANDKPINNSRYLKGFTNKFTEIVF
                     PMALGLKIKCTPVVDFGVEWRFIFANSDKLDAFKFSSRSDLTDLITGAPDKVSLSKMN
                     RDAYSYLNANFTFKFGKIGAQKEHSEWVNPLEAYMNVVDEKLKNQYKVKDADNDGVID
                     ELDWEPNTPEGAIVDTHGRTLDSDKDGYPDHTDPEPFSTPMLPIKDGVNIRPDGLTPE
                     QIQEVKNIINNEIKNISVGGWNLSLIFFDLDKYNIRVSEIPELYKIANIMKKYPEVKV
                     VAKGHTDVRASDPYNDKLSQNRVDASVDYLVNKFGIDRSRFVIEYFGEHVNMFPGAKT
                     EPEHQLNRRVEFFPYVPK"
     gene            28039..29427
                     /gene="mnmE"
                     /locus_tag="PLS16_02485"
     CDS             28039..29427
                     /gene="mnmE"
                     /locus_tag="PLS16_02485"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNJ59974.1"
                     /translation="MTKFQNDTIVAIITATGSAAIGVIRLSGNQAISIINSVFKGVDL
                     EKQASHTIHFGYIKNEATILDEVLVSIFKAPSSYTKENVVEISCHGSNYILQQVFMLL
                     VSKGARAAMPGEFTQRAYLNGRFDLAQAEAVADIIASDSEAAHKIAMQQMRGGISKKL
                     KALRTSLVEFAALIELELDFAEEDVEFADRTKFNNLLHQILLEIQVLSSSFKYGNAIK
                     KGIKLAIIGQPNAGKSSWINALMNDDIAIVSDIAGTTRDKIETTLDIQGVLFRLIDTA
                     GIRETEDTIEKIGVQRALETIENADIVMYIFDIINLTIEDIQKDLKLIHEKTNAPVLI
                     IANKADLITEQNITLKDHFKHLENLTYLSAKSNIDIEKAKDSIYNLALENYHFNSENV
                     IISNNRHYEALKNAEAAILVILKQLEQGISTDFIAQDIKTALRYLGDITGNIDVDKDI
                     LGTIFSKFCIGK"
     gene            29744..29950
                     /locus_tag="PLS16_02490"
     CDS             29744..29950
                     /locus_tag="PLS16_02490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNJ59975.1"
                     /translation="MKTSFSEFVKEKRKQLKLTQPELAERSGVGLRFIRELEQGKVSL
                     RLDKVNQVLALFGTQVGVIKNNEL"
     gene            29947..30261
                     /locus_tag="PLS16_02495"
     CDS             29947..30261
                     /locus_tag="PLS16_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HNJ59976.1"
                     /translation="MRKGNVYYKNNFAGIIAEIEDGYTFQYDEQFLNSTFVKSISLTL
                     PIQKQPFHSKILFPFFDGLIPEGWLLNIAVANWKINPRDRFGLLLTLCNDCIGAISIK
                     AI"
BASE COUNT        10258 a         5378 c         4023 g        10776 t
ORIGIN      
        1 caaggttcta gtcaaaaaat caatggattg aatgcctatc attttattgc tcaatatgga
       61 gaacaacaac aaagtcagga agctgcacaa attcttactg tacacacaac gttaattgaa
      121 tacaattcta atatttatat attcttagga ctttcagatt caaaagacta taatgcttat
      181 aaatccaatt tcacatacac tacaaatggg tttaagcaac taacagatgt aagcaaaatg
      241 aatgttttac ctgaaagagt aaaagtgatt actgtgaaca atactagttc attgagtcaa
      301 gttttaagcc aacacaatat tgcttcaaat agatacgaag aattggcgat attaaatgga
      361 atggaaacta ctacacaaat tccagctgga acattaatta agattataga aaaacgtaaa
      421 taagaaaaaa aatattatgg cagtacaaga tttaagtcct aaagaatttt tagcagcata
      481 caacaataca acaggagaaa aagaactaat tgacgttcgt aatccgcatg agattgaaga
      541 aatttcaatt gaaggacacg tcgcaataaa tatacaacat ccgagttttg tagaaaagat
      601 aaatgctttg gataaaaaca aaacttactt tgtgtattgt agaagtggca accgtagtgg
      661 gcaagcatgt aagtatatgg catctcaagg ttttgaaaac ctatacaaca tgaaaggtgg
      721 aatgttagaa tatgttgaaa ctgattttga cgactaatta ttcctgacta ggtttattca
      781 tattatctaa taaatctaga accatttcat caagcgttcc actacctgaa agttctccaa
      841 ttaaaccata gagtggtgca gtttttgtat tagatgattc tgtttttgat acatcttcga
      901 tacttttctt agacaatttt ttaatccaat tctctggtaa tatttttgct ccttgttgaa
      961 caattttttg ttttaaagca tcaccaatat tttctaaaga agtcgtttgt aattgctcaa
     1021 cggctttttt caaatcacta ataaattcat cagcataagc aacatgaaca taatttgcag
     1081 taacatgcag cgttgcaggt gttaattgtc tatctaaatg ccaacccaac tctgttaatt
     1141 catcacctaa tcgatagata tctaatttgc cagaagattt aatagaaaaa atcgttgtca
     1201 gaggatttcc tgtaacttca attccatcaa tagcattaac agcatttttc attttctgaa
     1261 ctgcctgata tgtttcttct gccatttttt tatagccaga actgcccaaa taattcatta
     1321 ttgcccaaga accagctatt gcaccgccag gtcgtgtacc tgaaattgaa ggcgatacat
     1381 acaagcctcc aatccattta gtatatacaa aaaattgttt ttttcgcttt tcagcatttc
     1441 tataataaac aacagatgca ccttttggac cataaccata tttatgaata tctaatgaaa
     1501 tagaacttac accttccatt ctaaaatcaa aaacaggcac attatagcct aattgttcta
     1561 aaaatggaat cataaaacca cctacacacg catcaacatg aaaccacaaa tttctttcta
     1621 gtgccaattg atttaacttt tctagtgggt caatatgacc atgtggataa gctggagcag
     1681 aacccacaat catcactgta ttgtaatcaa tcttagaaat atattgagcc atatcaggtt
     1741 ctaaaccatc aatacattta acagttcgta cttccaaatt aaaataatct gctgctttat
     1801 taaaagcagg gtgtgcagat tctggcatca caatatttcc tcttgtaata ttgttattct
     1861 tgctgaaagc atattttttt gcagcattaa cagcacaaat tatactctcc gtccctcctg
     1921 aagtcattgt accgactgct gattctggag cattcaacaa acttgctgta aatgacaaca
     1981 catcactttc catttgcttt aaactcttaa atactgtcgg gtttaatcca ttttcagaac
     2041 taaacaattt gtacgcctct tggacaacat tcgccgcgtc atcacctgga tagaatacta
     2101 aactaaaaac tctacctttt cgccagtcgg catctttatt tctcagttcg gtaagttcgg
     2161 agattataat atccttagag attcctgttt cagggagttt cttaaacata tatcaattta
     2221 tttcaatctt aaagttacac atttcaatct taaaataaaa tttaatacct aaaaacttgg
     2281 ataagcaata ctataaaaca atagattttt gtttatacca aataattaca aaaagtgtaa
     2341 ttagtataaa taataatgga acaacccatt tcatacttac caatactatt aaaaataatg
     2401 aagcgataac tgcgataata actaaaacaa ttaaactagt tgggtgaaat tttatataag
     2461 cattttcgta agcatcaggc atcatttttt ttaattttaa agaagctaat gaaataccaa
     2521 tactcaatgc cataatacca aaaattgtaa tatagctata aaattcaatc acatctttcc
     2581 caaaaaaata atccataatg cctattcttt ggaataactc tcctatcaag aaaactgagc
     2641 aaacgatggt aaaaaaaata tttactgcat attttggtgt tccatttgta cttacttctg
     2701 catatttttc tgggataatt tttgctctag ctaaagcaaa aacttctctc ggaataccca
     2761 ttccggaacc aataaaacaa gttaaacctg cactgattat tccaactcta ataagtatca
     2821 aagcccaatt tggcaaaata ctacttgcgg tattgaccat ttcaggtttt gtggtaatat
     2881 tctcgatgcc taccactcca atatatgcgg cagtaaccaa aatataaatc agccatacta
     2941 ttcctccacc gataaataaa gataatggaa tattgcgttt aggattttcc atctcctctc
     3001 ctagttccat aataatctga aagcctaaac tgatattgaa tgcaaatata atagacataa
     3061 aaaaactacc accttctgat ggtaatgaaa agacaacctg atcccaatta ttagctaatg
     3121 taggaattgc atatagcaac aatgctaata atgtttgaac tactaaaaca acttgcatcg
     3181 ctgcaatggt ttttactcca gtaagaaaaa cgccccaaaa taataccatt attattaaag
     3241 acaataaatg attggaaaat ggtatttgaa atacagtttg aaagctttct accaaaccat
     3301 aggcaactaa agcaacagac gctgctccac caaccaatgc ccaagaacta aacacaacgc
     3361 cagtcgtttt attaaaaaaa gtatttccat attcatagcc tccaccagca actggatatg
     3421 ctgccgacaa ttgaataatt ggaaatacat ttaacataca aactaccaaa gcaattgtaa
     3481 tactcagcca tgctgcacca taagctttag cacaaataag tggaatcagt acaaatgctc
     3541 ccatacctat aacacttcca acaacaaaag caactcctgt taataatccg atttgtttag
     3601 taagttgatg agttgtcata tttataaaat tagttaatca accaaagata aataattcaa
     3661 tttaaaatat atcagcatca agaaagtatt aaaataaaaa gccttacaaa agtaaggcta
     3721 gttcataaaa atcaaaacat cagtttgatt attttttaat cgttttgttt acggttgcga
     3781 caacatcatc tatttgaata ttaccttctt cttgactcac taaatcacca ccgtcattaa
     3841 aaacactaat gatatttgaa tgcgagaaat caattggaga aattctttta tatttcattg
     3901 ataaaacatt ggcaaactct tgagttgcag cttcacttga acgtaggaaa atccattgag
     3961 ggctatccat cttatttttc ttagcaaatt ttttaagatt ctctggagta tccgtctcag
     4021 gatcaataga aactaaaacc aaggaaactt tatcaatatc ttttctactt attttctctt
     4081 caatgcctct catttttgcc accaaaattg gacatgcgga tttgcatgtt gtaaaaatca
     4141 ttacaactac taaagttttg ccttgcaaat ctttcaattg taatttctcc gcatcttgat
     4201 tttcccactc tgaagtcaaa ttaaaaatcg aactctcaga aatttccttt tttgatttac
     4261 tattactatt acaagatgta aataacaaag taaataagaa tatataatta aaaattttca
     4321 tactatttaa tatcagtttc tacaatcgtg tctttagcac atctaaatcc taagttagca
     4381 attgtaaaat ttgctttaat agaagtacgc aatgcaaaac gcataaatgc tgcataattc
     4441 aatacatctg ttgcggcagt tgcaccgcca gcacaaaaaa gatttttatc attaaactct
     4501 ccatttcttg aatctccagt agtcataatt gaattaaaat catctgtcca ttcccaaatt
     4561 aaatcaaaca cattatatac gccataataa ttaggagggc tttgctttac tgcattaaat
     4621 tgtcggtctt tttgaagata taaatctacg atattatctg aataagattt cttttcacga
     4681 gcattaggta cttcagcatc agccatggca acaaattccc attcatctaa agttggcatt
     4741 cttttgccaa cactttctgc aaaggctttt gccgcaaacc aagatacaaa acagattgga
     4801 gcatcaggat ttgcaccttt gggtagttcc gtatcagaaa cccagtctct aagatagcta
     4861 gaatcagaat atactttttt gatatttgat tttctccatt gtggattcgc tattacaaat
     4921 tctaaaaatt cttgattggt aactggacgc tcatctatca aaaatggagc aactgttaca
     4981 tttacttttt cagagccgta aaacggttta tattccccac cgggaatata aaccatcttt
     5041 acgtctgttt gtattttgac taatgtatcg actaagtttt cttgacttgt aagttcttca
     5101 gtatttacat ttccttttgg attacaagaa actactaaaa gagctattaa taatataaaa
     5161 aacctattta cttccattga tcaaaaataa taattattat tcttttacta aagttttcgc
     5221 attaaatact tttggatttg catcaccagt aacttttaat ttaccaattg cacctttatt
     5281 aaatgctctt gataaagagt ggtcaacaat cacaaattct ccaggcactt cacatttaaa
     5341 ctctacaata gcagcaccac ctgcaggaat catagttgtt tgaatgttat gagaaattgt
     5401 agttccacct tctaaatata cattatcaaa aatttctcca attacgtgga aagaagaagt
     5461 taagtttgga ccaccattac ctacgaataa acgaacagtt tctccaactt tagcctcaat
     5521 catattagca cctaataaag cacctttggc accattaaac aatacatagt caggattttc
     5581 tgcaacagct ttatcattat caaattctaa taaccctttt ttcacagctg atgcctttgt
     5641 atagaaatct ccttgcataa tataaaattc tttatctact ttaggaagcc caccagctgg
     5701 ctcaactaag ataagaccat acataccatt tccgatgtgc ataggtactg gaggagctgc
     5761 acagtgataa acatacaaac cagcatttat tgctttaaat gtaaatgatg cttcttgacc
     5821 aggagcagca gtagtagccg cagcaccacc accaggacct gttacagcat gcaaatctat
     5881 attgtgtggc aatacactat tagctgcatt tttcaaattc aaagtaacct catcgccaac
     5941 tctaattcta ataaaacttc ctggtacagt attattgaaa gtccaaaaag tgtaaggaat
     6001 actatcagcg ataaatcctt cgatttcagt agtttctaag tttactatcc atttttttgg
     6061 acctctatca cctactggtg ctggaacatt tggagctcca gtaacttctg ctacttctgg
     6121 gtcacctgat gctaccatat caacagcaac atctgaacca gatcctgaac tgcttggagt
     6181 agttttacaa gcagaaagtg cgaaagcaaa taatgcaatg gcacataaat ttaaaaagtg
     6241 ttttgttctc atattaatcg aaatttaaag atttaaaaat tagttttaaa aaatgttggt
     6301 gtaatattta agcctaacca catccattgt tgataaactt ttgcattagg tgtatttttc
     6361 aaaaagttga tacttgaatt tgctaaataa aatgaatatc ctcctgttaa acctacaaat
     6421 ttatgtattt tataagagat tcccaagtct aattcttgtc ctaagttttt agaaaatttt
     6481 gtagctccat ctattaattt atttggtgta acaaattggt ggaatgccat atttaaacct
     6541 aagccttttt ctgttttgaa atttattttt aaataagtat ctgaaagtcc agcattttta
     6601 tgaggatttc ctgaatagaa ataatccata aaaccataga acttatgtcc tgtgtggaaa
     6661 tacggcgtaa aaatatggct tttcttttgt gtagctccaa cattattccc acttaccata
     6721 tcacttccta ttcctatatt ccatttttta ttgatattat atactaatgc cgcactcaac
     6781 atatatcctt gatttttgat attagttaag tcttttccca tttgataata ggccgcaacg
     6841 ctaacattta atttacttcc attgtaatta taattagtac caaaagtttg agcataattt
     6901 gtaaccgtat ctttagttgt tgtaatagca ttttgaaagc ctaaattaga aactaagaaa
     6961 gtgatttttg aatgttcttt aattggaact gaagcccaaa cggtttgcat ccatttgtat
     7021 ggcaatgcat tatttgtaga aaacaaactt cctgctggat tgcttaaatt attattatac
     7081 aatgctctgt aattttggtt aaatgcaaaa tatcctctta attcatactt ttctttttta
     7141 tacaatatac tcaatgcatc atgcactcga cctccttgag cccaatctaa ttcaccgaag
     7201 aaacgttcat catctagtga aattacttga cgacctaatt tgaaactcca ttctggagat
     7261 gcatagactt gagcccaagt ttcaaaaata gaaaacttac ttcctttatc ttctgcacct
     7321 tgagtcatag tagcttgacc ccatacactc actgcttgtg gtgtaattct taaagagaat
     7381 acatttttat aagcataatc aaaagtcaaa cgaattctgt cgcttactaa agctgctggt
     7441 ttttcatttt tcaataaagg acgaaatgct ccatctctca actcaaatct tggcctaaat
     7501 tgacctgaca tagaaaaggt attatttttt acagaatctt gttcttctgc attagagaaa
     7561 tttaccacta aaagcaatac tataaaactg agaagttttg aagtattata taaattatta
     7621 aatgctttca taaaattgga tttaattgtc ttattttata caacaaagtt atagaacaac
     7681 ttttaacttc attatgatat atgctttacc caaatatgag aaatatcagg aattataaaa
     7741 atcaaggaaa caaaaacaaa gcaaattcat aacaagcaaa taatcaataa attgtaagga
     7801 attaatacct actaaaaaca attaaaataa aaatgagcta ttaaaaatag ctcatttttg
     7861 aatttattta atatctatct aatagtaagt taatattcca atgctcttgg gagtgttttc
     7921 gcttgttcaa tccatttttc cacttctttt aaagatggtg ttctgcgtgt taagtttttt
     7981 tcagcattta ctgtttccat ttttttagtc aaattatccg cttttctttg agctaattct
     8041 gcaacagtat ctactccaga tttttccaat aattctgagt attcttgacc aacaccacta
     8101 attctaaata aatcagccat atttgcaaac ctaagaatta atttttctgc tattccagat
     8161 gcttctgcta attgcttacg ttgtttaggt gttttagaat tttctaaaag tttgtctgta
     8221 ttgtttatac ctgtatctct tagttgctta cctaaaacag aaccaatacc ttcaatttct
     8281 tcaattttgt aatttgccat gattaattat ttttaagtta ttcaaatatt ttgtgttctt
     8341 atgtttcagc atttcgaata taataaaaac aaacttataa agtaactgat taaactattt
     8401 taagtttttt cattttgctt tcggtaattt attatctaaa cgtttaaacc accgtaatag
     8461 tttactatca tctgcttttt ctgtatgcca atctcctgct tcttctctac taatatcaga
     8521 accgaacatc aaatctgcca tactgattgc ttcattggta ttcactgcac ctccaccaaa
     8581 ataagtatct attaaatatt ggtgtaattc tttgggcgtt ggataattat gaatcacatc
     8641 cattttaatt tctccattac caattaaact ttctaatttt gctaaaaatt ctccactcat
     8701 catactatca aatccttgct ctctataagg tttttggata tctaaagatt cgcctgcttc
     8761 taagcctaaa attttagctg cttcaatttg cataatcact aaaagtgttt ctctttttac
     8821 ttgtggttga actatatttt ttgaagcgta cttaggtttt gcaatgcttt cttgttttat
     8881 acttttatta tcttgatttc ttaattttgg ctcaacatct ccgattctat ttggattata
     8941 ccaataggat ttccactgcc atgcataagt tggcaatttc acacgattaa ttcgctttcc
     9001 tatatataat aaactccaat ctatattaaa accattactg aataaagttt gcaatatatt
     9061 gtaaaattgt tgtacatcat cagtttgtgg tttggttgta tagagccatg aaatattttt
     9121 atcagacaaa atatttttac ctaaagtgga caaaactgga tttgctccac attcgatata
     9181 aaattctgct tctattgtat tttggatatt ttggatactt tttaaataat ctacaggcac
     9241 tcgaagttgc tgtgtaagat aaaatgaatc aatactttgt ttgtctaata tttttccatc
     9301 aatgtttgat gctaatggaa tttgtgcgaa agaaaaatta atttcattac aaatattttg
     9361 aaaatctaca aaaatacttt ccatatcttt tgaatgaaac gcatgagaaa cttgtaaagg
     9421 tactgcacga atattttgtt ctttcaatct ttctataaat atagcgattt gttctttatc
     9481 gcctgtaatt gttgtattat ttgtagtatt ataaccagca atttcaacaa tattatcgtt
     9541 attcaaatca actattgttt gagcatttgc aaaaacagca gccattccgc cattgttatc
     9601 aggaatattt tgcattaaat tacctcgcat cgaaacaatc ttcaacgcat ctttaatttc
     9661 taaaacgcca gaaattgtca atgcaactaa ttcaccaata ctatgtccaa ccaacatcgt
     9721 cggttgtatt ccatatttca ttaataattg agctaatgca tattctacta taaaaattgc
     9781 aggttgcgta tatttcgttt ggtgtatcaa gtgtttatct tcgtgtttcc acattacatt
     9841 tttgatagac tcacctaaat attgttttaa ttcttcatca caaaaatcta tagtattctt
     9901 aaaatactca aatgatttgt aataatttct acacattcca aaatattgag aaccttgtcc
     9961 agtaaattgg aaaacgattt gtccgttatt atcaatattt tttgatgtaa ataaattagg
    10021 attttgtttt gaatctataa aatcttgtaa tgcctgaata atatcatttt tatcttttac
    10081 aaaaattgac aatctatgct caaacaaatt ccgttgttgt agtgtataac aaatttcaga
    10141 taaagtgtgc ttatcaaaaa cgcttgtatc tgctaagtat ttaattacga catcctttaa
    10201 cgcatctggc gttttagcac tcaaattaaa aataaacgga gcattaactt cttctgattt
    10261 ttgttttata tcagcttctt ctaaaatcaa atgagtattg gtcccactat aaccagaaag
    10321 gttaatcgca gctaatcttt tagaaccatc aaactttagc caatctgtat tttctttggt
    10381 aatatttact tttatttttt gccaatctac atctttattt aattcgtgta aatggatttg
    10441 agcaggaatt attttatttt gcatagcagc tattatttta aatagcattg gcattcctgt
    10501 acatgcttct aaatgaccga tattactttt gacagaacct acttgaattg gtctttgtcg
    10561 atttttataa gcttgatgaa tcgcttgaac ttccattgca tcggtatatc tattaccaat
    10621 accatgaact tctacataat caattgaatc aacgcttagg tttgcattag acaaggctgt
    10681 tttaatcgta tctacttgaa cttgcggatt aggtgcagta aatccattgc tggttccatt
    10741 gtgttttaca gcagcacttt taataataat ttctatatta tcattattat attctgcatc
    10801 ttctcttctc tttaaaacta aaacagatgc accctctcca cgaacgtaac catctgcatt
    10861 ttcatcaaat gctttagttt ttccacttgg agaaaccgaa cccattttgg ttaatgcaac
    10921 aaatatttca ggtgtcaata ataaattagc agatgcaata accgcaacat caatctctcc
    10981 gttattcaat gcttgttgtg cttgatgaat agctactaaa actgatgaac acgcagtatc
    11041 taccgaaata ctttgaccta caaaatcaaa tgaataagat attcgaccac aaatagttgc
    11101 aaacggaatt ccagtggcat cataaacatc aatatcttct aaatttccac tacgtaaacg
    11161 agcttgagca taatcactta aaccaactgc cacaaatacg ccaacatttt ttgttttgag
    11221 ttgattagaa ggaatgcctg cattttcaaa tgcttcataa gttaattcta ataaaatcct
    11281 ttgaattgga tctatagatt ttgcttcacg aggtgggatt ccaaaaaaat ctgtatcaaa
    11341 taaagcaata tcttgttcta aaaatgtacc aaattttgta tttgtttttc ctgcttcata
    11401 ttggtcttcg gaataaattt catcaacatc aaatcgagca attggaattt tagaaactgc
    11461 atcttttttt tcatacaata aattccaaaa cttctgtaaa gtatctattc catttgcaaa
    11521 gcgaaaggca tacccaacca cagctacatc tgtatttttt ggaatttcag atgaaataat
    11581 ttcattttta attaaagact gcctagactc aatctcttgt tctaattgct ttattttaat
    11641 gagcgacttt ttaagtagct cttgcatttg tttaatttgt acttcactca tcttttaaat
    11701 tttttcgttc aactgcttca ttaattcttc taaactcaaa tcatcaacct ctgcctcaat
    11761 acttttatta tcttttgaga tttctgcaac ttctatcctc tctgtttctt tttcaaacaa
    11821 attcaattct tcagcgataa aatttgcata tttttccact gttggatggc tccaaactga
    11881 tgctatattc aaattcaatt gataatctac ttgtatttta tttttcaatt gcaatgccat
    11941 taatgaatcg attcctatgg atttgaatgt atcatcttct ttaatttttg aagatggaat
    12001 ttttgtaatc tgagcgatat gttgtttaat attttctttt attgataaaa tgcttggctt
    12061 ttcttgaggc aattgttcaa caaattgagt tttatcttgc tcaacaaatt gagtataaaa
    12121 tagattattt tttacattat tgtaatattt tttccaaagc gtaaaatcaa tttttaaagg
    12181 aataaattgt gccgcctcaa cagaaaaaaa ttcttcaaaa tatttaggta attcattggg
    12241 cataattaaa tccaaacctt gttctttcaa gtttgcacca cgctttgtat cttgagcggc
    12301 caaaccaatt gttccaatat ttccccaatt cacagcaatt gtatctaaac caacttgcat
    12361 tctatgtttt gccaagttat ccaaataata gtttgctcca ctatacaccg cttgtgctaa
    12421 tgttcctaaa atgcttgcag ctgatgaaaa gaaaattact tttgattgta atctatcttg
    12481 acataaagca tcaatatttt gagcaatttc aacttttgaa ttaagaataa tttggatgct
    12541 ttgtctatca atatcctgaa ataaagcatc tcctaaaact gcagcagcgt gtacaatcgt
    12601 ttgtactttt ggcattgctg tttctattgt tgaaatgcag gtttttgctt ctacaaaatt
    12661 agagaaatca gctttcagta ctgccacttt tgtattattt tgcttcataa actcaatagc
    12721 ttcttgagtt ttatctttca atcctgaacg acttactaat gcaatattat taacaccttt
    12781 tgtacttagc cattttgcta attctaaacc caagccactt gttccaccaa taattaaaac
    12841 cgtttgttct ttattaaaga aataattttt atggggtaat ttagctttaa aaatttgcaa
    12901 ttttggttga taaaccaatc catttcttaa cgcaatagat ttattttgag ctggtgtatt
    12961 gaataagtga atagcggttt ttatatcatc ttgtttccaa tcaaaaggca agtcaattgt
    13021 tctaaaatca atatctaaat actcattatg taaactttga acaatacctt gtacgattga
    13081 tgccgttgta ttcaattgtt tatctccgtc taaaacattt gctccattac ttagtaaaat
    13141 aatctttggg cgtttagttt cattatcata aaagcgaact aaattttgta aaatagtaat
    13201 cgcttgaagt tttgatatgg tcgttcctaa taaattaaat acaacgatat ctatatcttt
    13261 agaaaaatct gattgatgaa tttgttgaac tttaacatta ttttcttgct ctaaaatttc
    13321 ttgtaaaaat gaaacacctt caccattcaa taataaaata ttttgttgtt ttaaagcaat
    13381 agaatccata tttatagatt catatacaat ttcaaaagag ggtaaattat tgtttaatat
    13441 ttcgggtaaa gtagtacttt ttgttgatga taaattaggt tgttcatcaa accaatagcg
    13501 ttctttttgc caagcataat ttggtaattg tacgaatttt ccaatctcag gataaatatt
    13561 tttccaattg atagtataat tcgtttcata taaagcacca tagtttttat aaaaagattc
    13621 aaaatcattt ttatctctca aaaaagaagg caatgcaatt gatttagatg attgcatatc
    13681 aaaattttgt tgtaaagcat gcaataaagt tgggtgcgga ctcatttcaa taaatacaca
    13741 tttttgctgt tcaataattt gttctacact atctttaaat cgaacagttt gtcttaaatt
    13801 atttttccaa taattggcat ctaaacttga accatctatc ttttttccat atactgtgga
    13861 atagaaagca atattagaat ttataggttg aactgaatct aaagcaatag ctaattcctc
    13921 atcaatttta tccatgtgaa tactatgaga agccacatct accttaatca ttcttgcaaa
    13981 ttttccttct tgttccaatg tagattgcaa atgccaaatg gactcgctat cgcctgaaag
    14041 cactaaagat tgttcgctat ttacaactgc aatagacaac ttatcttgaa ggttttcaat
    14101 tctttttttt gcttcactaa aggtcaaatc agttgccaac atcaagcctt ttccacttac
    14161 ttgcttcatt aatttactgc gtttacaaat aattttaaca gcatcatgta aacttatatt
    14221 ctctgcaaca taagcagccg caacttcccc taaactatgt ccaattacac catttggaaa
    14281 aatgtcttta cttttccata aatcagctaa tgcaatacca attgccacca aaataggctg
    14341 aacaacatct aattgattta agaattgttc atcatctcca ttcaaaacat caaacaaatt
    14401 ccaagataca tactttgata attcattatt aaatgcttct aaagatgcct taaaaactgg
    14461 ttcttgagta attaaatttt gagccattcc caaccattga gcaccttgac ctggaaatac
    14521 aaatatcgtt tttacaaaat catcattatc aaaaattgta gtttgatttt gaactcctaa
    14581 ttctattgta tcatgcattt gttgaatcaa ttcttgttca tcaactgcta caaaaacttt
    14641 tctatactta aaatcagcac gccttaatgc cgccattgca cagatatctt gcaaagtatg
    14701 ttttccttct tgaataaatt gcaggtattt ttttatcaat tcaactaaag cttgttcact
    14761 ttttgctgaa attggcaaca caaaaatatc tgttctcaca gcattatgct cctttaaaac
    14821 agcatcatat ttttgtacta ccacatgagc atttgttcct gtaactccaa aactacttac
    14881 accaacattt tgtattgaat tggattgatt ttcttgcact acttgaacag gtaaactttc
    14941 ccaatctact aaactattta aagtatcaga atgtagattt ttaggtaaaa tctgatgatt
    15001 taaagctaaa acatttttta ctaatcctgc taatccagca acactttcag tatgtccaat
    15061 atttgatttt atagatgcta gattaacctt tttatttgaa ttatttccaa aaacatttgc
    15121 taatgcttct aactcaattg ggtcgccaat ttttgttcca gttccatgtg cctcgacata
    15181 atcaatggca tcaggattta atccagcatc tttcaatgct gcttgtatta atttagtttg
    15241 tgcagaaaca cttggtgcag taaatccatt actttttcca tcttgattta ctgccgaacc
    15301 tttaatcact gccaaaatat tatccttatt ttgctctgca tctgacaatc gtttcagaac
    15361 aatcacgaca gctccttctg agcggacaaa accattggca ttgtctgcga atggacgaca
    15421 ccgagaatca cgagataagc catttaatgc tgtaaaacaa atggacaatt ctggttctaa
    15481 aattaaattt gcagcaccca caattgccat atcactttct cttttcctta atgattgaac
    15541 agccaaatgt gttgcaacca aagaggacga acatgctgta tctattgaaa ccgatggccc
    15601 ttgaaatcca aaagtatatg aaatacgtcc tgcattggca caaactgctg aacctgtata
    15661 ggaatatgga ttaactaaat tataatcacc acacctgaaa tgttttttct gataatcaat
    15721 acttgtaata cccacaaata caccagtttc tgaatcaact aactcagcgg tattgatacc
    15781 tgaattttct agtgcttctt gagttaattc taataataaa cgttgctgtg ggtcgaggct
    15841 ttctgtttca attctagtta aatcaaaaaa gggtgcatca aagacttcta tatcttttaa
    15901 atagccacct tgttttaaaa tattttttcc aacaccacct tgttcatcgt aaattttact
    15961 gttatcaaat ctactttgtg gaatatcttc aatagcatca atattattaa ttaataattt
    16021 ccataaatca tctgcatttt ttacatttcc aggtaaatgc atagccattc caattattgc
    16081 aattggctct gaatgtttat tacgctcaat agtcaattgt gccttcaaat ttttgatagc
    16141 acttaatgct tgttttattt gttcactata tttctctgta tcggtcataa taaatccttt
    16201 agctcatcat ctaatagttt acttaaatct tctaattctt gttcgttgtt ggaatgggtt
    16261 aaagttgtag gctgttgttg gttttctgtt gatggctcgt tatcgtcaat tgataactgt
    16321 gctatattaa tatcttgggt tttgggtgtt tcattttctt tagaagtaac aacaaattcc
    16381 tgtgttctaa gttcagtttc tacagtacta ttttctttat taaaactcaa tttatcaatc
    16441 aaaaactttg catacgcttt aattgttgca tgagtccaaa aagcagtaac agacaattta
    16501 atttcaaaaa tatgttccaa ttgattcttt aattgaatag acatcaatga atctattcct
    16561 aaagatttga atgtagagcg tgtacttatt ttatctattg aaatcttagt aaccttagca
    16621 agtgtttctt ttattttctg ttcaatttga tgttctaatt ctgttttgtc attaatcagt
    16681 tttagttcat caatataatt tgataaaaca gttgttgttt tatcatcttt tgaatccgtt
    16741 aataaactga aataaggatt attttttgca gtactataat tttgttgcca tttttgaaca
    16801 tcaaatttaa aaataccaat atttgcatct tcaatattcg caatatcttg gaatagatta
    16861 gtacaatctt ccgccaataa aggttcaatt ccttctgctt tcagacgctc agaacgattt
    16921 tcggaaactg ctgctaaacc aacatcagca actgtactcc attgaatact taatgcagct
    16981 aatttttcag ctttacgttg ctgagctaat ccatctaaat aggcattggc tgccacatag
    17041 gcactttgtc cgggcgaagc aaacaatacc gccgacgaac taaacaatac aaaataatct
    17101 aaatcaatat ttttggttag ttcatgtaaa tataatgcac caaatacttt aggtagtaat
    17161 acattataga atttatcttc agtcaaattt ataatagatg catcatctaa taatccagct
    17221 aaatgaaata cacctttcaa tggattttca acagggatat tattaaaaac aatttgcaaa
    17281 tgtcttttat tgcttacatc agcttgatag gtaataatat ttacacccac atttctataa
    17341 ttttccaagg tgctttctat ctcagaactt ggtgcttttc ttcctaataa aatgatattt
    17401 tttgcacctt gctccaccaa cattttcgta aattgtaaac ccaagccgcc taagccacca
    17461 gtcaataaat aagttgcttt ttcatcaatt gtaaactccg aatttttatg aatttctaaa
    17521 tcttcttgat gctctatgtt gataacaatt ttaccaatat gctttgaagt gtttattaac
    17581 ctaaatgcct cttttgcttg agctatattg aatacattga atggcaatgg ttctagttgc
    17641 ttttcttcaa ataatgcaga aatttcatgt aataattcgc ccaataattc tggtttttca
    17701 aacaataact tttcagcatc aaccataaaa taactcagag actttcggaa cacttccatt
    17761 ccaatttttg agttttcaaa aatatctttc ttcccaattt caataaatcg accaaaatta
    17821 tttaaaagct gaagactttt atacattgct tctccaacta atgaatttaa gacaacatca
    17881 acaccttttc catcagtatc ttttaatatt tcatcaaaaa aatcaactgt tcttgaattg
    17941 taaacattaa caacaccaat tgattttaaa taagttctct tttcttcact gccagctgta
    18001 gcataaatct cgcattcata taatttacat aactgaatgg ctgctaatcc gactccacca
    18061 gttgctgcat gaattaaaac tttatcgcct tttttaatac gagctaattc tatcaaacta
    18121 taataaacag ttaaaaaaac tgctggtatt gttgctaatt cgtcataaga ataaccttgt
    18181 ggtactttac acaaagcatt agcatttacc aaagtatgcg aagccaaagt atcataagcc
    18241 attccataaa ccacatctcc tacctgaata tgatgtacgt cttctccaat tgcttgtaca
    18301 attccaacac actcaatacc taaagtacag aaaccatttt ttccacttgg acaaataccc
    18361 aaaacagaaa gcaaactcat gaaatttaca ccaacagatt ttatttcaat caaggcttga
    18421 ttagcagaag gagctgaaaa ttcataggca ttagctacta aattatctat caatcctact
    18481 tgttccattt catatctaaa cggaatattt ttagatataa cttttgattt atgttgttga
    18541 gcaattaatt tatgacttag tcttgtggca taaaactgac catctctaat ggcaatttca
    18601 ttttcattat tatcctgatt taataaattt ggtagtagca ataaatcttc cgatttttca
    18661 atatcaatta cagacggttt taattcaaaa ttttcattcc aaagtattct aacaaatgcc
    18721 ggaatttgat gttgtaatgg cgaaacaact tcctgttgag taaccgcaat tgcacctttt
    18781 gtaatgcacc acaaacgaac ctctaaattt aattcaacaa cagcctgtac aacttctgta
    18841 atgcctataa tatttctttg ttggaaagag aaaataccgt cttctttatc ccactctaaa
    18901 ttttcatcaa cacaaaaaac caattgtgtg tttggcgtaa attctatttg tttgaatatc
    18961 tgcttcaaaa ttgcttttga ataactttga atttcaataa aactaatagg gcgtgtttct
    19021 ccaaatgctt gagcaataat ttctttattc tttaaattac ctaatagaac aacggcatca
    19081 tttgagctac tattctgaat aggaattttt gttgattcta attcataaaa aagattatcc
    19141 gcagaggctt cacttttcgc ttgatgaatt actctaaatt cgacaccaac agcttcaaca
    19201 cataaatcat ctgcttcata aatccatagg ttacacacca atttcgtttt atcaaaatca
    19261 tacacctcaa ctttggattt tagtttggat gttttgggtg ctttatagaa tttatattga
    19321 ctaatagaaa atggcacata agtagcatca tttttcaagt aaattggtgc taaaatcgtt
    19381 tgcagacatg catctaataa tgttgggtga ataatataat cggcaatatt gatattgtca
    19441 ggaagttcaa ttatagcttc aaaaacgctt tcattatgat gtattttttg aattccctga
    19501 aaaccattac cataatttaa gttacttatt cggcatgcag aataatgcgt ctctttagat
    19561 atagcttgtg ctaattctgc aactttatca gagtttttat aaactaaagg tttgaaaata
    19621 atatctgtgg aaagatgttg taacacttca tcttctttta ttgaatttat tttaagacta
    19681 tagaaattac caatagcttg ttgcaaagta atttttaaat taatagcttc ttcttgagat
    19741 aattctaaac tttccaagat acacagatgc tcaatttgga attgctgttc tggatttact
    19801 tctgtacaac aagcttgaat tatttcaata aaaccagcag ctggaaaaat aattttatcc
    19861 ccaactttat gttctgataa ataggtaaat tcttctaagg ataagttagt ttcccaaata
    19921 tgtgtagtaa tattaccttg actaaaagca taatttttca aaaaaggatg tggcaaacca
    19981 ttatttacct ttctatcttg attataaacg cttgatgttt gaatttcttt tatccaatga
    20041 tattcacgtt gccacggata tggaggtaat ttaatttttt cgtattggtt tccataaaag
    20101 tttttccaat caataaagac accattacaa aaagcttttg caatttgctc tgtaaaactc
    20161 aatatatcat tcttattttt ctctaaagaa aataagacaa ttgcttctga tttttttata
    20221 tctatatttt cttgaattgc agaatttaaa acaggatttg ggctaatttc aataaaaatt
    20281 tcaattccct catctattaa ttgctgaata gcttctgaaa actgcaccgt atttctcaaa
    20341 ttatctaccc aatagtctgc ttgaatttgt ttagcatttt gtatttcagg atgaacggtt
    20401 gattggaaaa taatttcact ttcttttgct tgtatatttt gggtcgcatc taaaagacga
    20461 ggttttaaaa tatccatttg ttgactatgt gaagccacat caacttttat caaacgacta
    20521 aaaaatcctt gttcatctaa agttgaaata atacgttcaa cttctgtttt atctccagta
    20581 attactgtag attttggact attgacaaca ccaatattta cgttatcaat agatgattct
    20641 aaaaatggtt ttaattcatc aacagccagt gcaacataac ccattgcacc tttacctttc
    20701 tgttctttca ttaattggct acgtgtgcat attatatttg ccgcattatc taatgaaata
    20761 attccagcaa tataggcagc agcaatctca cccatactgt gtccaacagc tgcattaggt
    20821 tgaacaccta tgctcatcca tagtttagct aatgcaattt ctatagcaac taaagttggt
    20881 tgaatgatat caatttccga taaactttct gtatcttctg aaaataaaat atcggttaaa
    20941 ttccattcaa cataattttt aaaagcactt tcacaatcat caatagcatc tttgaaaaca
    21001 atttcatgtt ggtacaattc tttgcccata cctaaccatt gagaaccttg tccaggaaat
    21061 acaaaagcta tcttaggtaa tttagcagta ctgtagccgc taaataattg ttcattattt
    21121 gttttatcta caatagcttt aaagcctagt tgaagttcat taaaatctga gaaaacaatc
    21181 gcatttctct gttgcaaatt tgctttatga aaagcaatat tctttgtaaa attcagataa
    21241 ttattttcat ttatttgtga ttgataaagc tttacataat ctattacagc ggctgaatta
    21301 tttgctgata taggtaaaat attatatttt ttatcaaata ctatattttc tgaagattta
    21361 ttctcaaaag attgaacaat tacatgtgca ttagttcctg taattccaaa tgcatttaca
    21421 ccagcaatta aaggttcgtt ggtttcagtt tcccatgcaa ccaattccgt tggaattacc
    21481 acattcatat tttgccaatc aatatctgga ttaggttcat agaaatgtaa atttggcgga
    21541 attgttttat gcttcattgc catcaaaacc ttaaacaaac cagcaaaacc tgctacacct
    21601 tcagtatgtc caatatttgt ttttacagaa ccaataaata tactattagc accatttcta
    21661 tttttagata aagcattcca aattgaagaa atttctgcag ggtcaccagc ttttgtacca
    21721 gttccatgag cttcaatata ctttacttta ttgacatcaa tatttgcttt tgagtaggct
    21781 tcctccaaca ttatttgttg tgtaattgca ctaggtgcca gcatatattt atcagatgca
    21841 ccatcattat tacaaacact tgctggaatt acagcataaa tttcgtttcc atcatttatg
    21901 gcatctgaca atcgttttaa gataattaca gcacctcctt ctgaacgaac ataaccatca
    21961 ggatttgcag agccaaaatg acaagctcca tattctgata ataagccaga gcgagaataa
    22021 ccttttgaaa taaaggagtc cactattgca ttagcagctc ctgcaaatgc caaattacat
    22081 tctccagatt ttaaactttg aatagctaaa tgaatagcta ctaacgaaga agaacaagcc
    22141 gtatctattg taattgaagg tccttgtaaa ttataaaaaa atgataaacg tccagaaagt
    22201 ggataacgcc cagagccagt ggtagaatat acatctacat catgtagtgt ttgggctaag
    22261 cgttgctcaa aatcattcaa ccaacaacca ataaaaacac cagttttaga accatataat
    22321 ttctctaaag gaattccagc atcttcaaca gcattgaaag aagtttctaa gagaaaacgt
    22381 tgttgagggt cgagtttatc tgcctcttta ggtgaaatct ggaaaaaatt ggcatcaaaa
    22441 tactctatat cctttataaa accaccacgt tttgtaacga ttttaccatt tgctctattg
    22501 ttattaacta agttgtcaat attagggaaa cgattatttt caatatcagt aatagcatca
    22561 attttattac taacaatatc ccataaacgc tctatatcag atgtatctcc agggagtttg
    22621 caacctacac cgattattgc aatcgcatca tctattatac ttatttgttt atccatttct
    22681 tgaataaaaa taagaaatta aatctttaaa gttgtaaaaa ctaatgtgaa tttagttttt
    22741 cggccaaagc attttcttca cgtcctataa gtacggcttt tctaagcatt aaagggataa
    22801 tcactaatac aatgctaagt gctaaaccaa ttaaactata taacattgta cttttatatt
    22861 taggcgttgt tgcaaaatca ggatgatcta caacacgaac tggacttgct tgtggcttca
    22921 tactcgtttt agcagattcc ttagtagcaa tagcatctgc atacatcatt tttaaattag
    22981 aaacttcttg ccgaataaaa tcttgatcaa aaatgccttc ctttttcaca ttaaaaatac
    23041 ttctatcttg gtcttgagct attttctttt ctctccaatc aatttcagat tttaatgaat
    23101 caattttctt gttgatagtt gtaaaagaaa tagatgcact tgaaacctgt tcattcatat
    23161 aatattctga aactttattt ataatagttc ctgctaaatt tttggataat tgataatctg
    23221 gcgtaacaat ttcagaaaat atcagaccag aagtaacttc atattctact tctacaattc
    23281 catcaataaa catagaataa aaaatattag caatactatc ttcatctcta gaaaaggaat
    23341 ttatatcact ggcagtaaac ccaaagtttt ctttaccttt tgtcgcttta aaaccttgat
    23401 ctaaaccata aacacgcatc atttcatcac ccaacttctc cactttacca tcaacagaaa
    23461 catctgttat catagcatta taaaatacta atcttgaatt atataaaccc gctaataaat
    23521 caacggttgt tccacttgct ccaaaaccta aagaggatgc taaagaaatc aaaccagagg
    23581 ctgcaatacc tttaggatca actgtattga atgaggtaaa agctaaatat tcaggctctg
    23641 catttttagc ataataatac aaaccataag tcaatccgcc aacaccaata ataacaatat
    23701 aaaatttttg tagtagatat ttaaaataaa cgccaataga gtcggtaata tcaactaacc
    23761 ttacatcttc ttcgttaaat tcttgaactg tggacattca ttattttttt aagaatacaa
    23821 ttaacatagc agttgacaaa gcagaagtaa ttcctgctat caaactaggt aataaaatat
    23881 ttaagcttac tggttctctt gtcataggag gcattttcgg ttttggttct ttttgttcaa
    23941 cattcaccaa agcaccttct acatctattg ttggataatt tttaatccct aaaaagcttt
    24001 tcgtatattc tactttacca ttcggataca aaaccatcgt attttttcgt ttagcttcct
    24061 tcttaaagcc tccagcatat tttttaacat accatcttgc actttttcca gcaacaaatt
    24121 tgccactaat tgtttgtgca ctatctaaat ttggataacg aactgcccca ttaattctaa
    24181 ccaattgatt tattgtagga atttcaataa tatcaccatt ttttacaatt aagtttgcac
    24241 gagaattagg gtctttaaat gcatctttta attcaaaaac tgtcttaaat tgattcatat
    24301 cagaacgaat taatttagca ctttgtatgt gagcatattg ggttagtcca cctgctcttt
    24361 ctataatgtc taatacacgt tcattcttat ctaaaattgt atatgagcca ggataaacaa
    24421 cctcaccact gatagtcatt ttcatttgtt ctgcaaaacc aggcgtttta cgaacatcta
    24481 ctaaatccat tggagctaat ctaaatgctt tagatgcttc atcaatttct aaatttggtc
    24541 caatttcaat attttgaaca acaactcttt gcggaataaa ttttttaccc aaagaatctt
    24601 cgtcattaac ttccaaaact cttgaaattt gaatcttaga attagcagcc tctgttttta
    24661 atccaccagc ataaaataat aaatcattta aagttagacc atcagaatac tctaaaattg
    24721 ctggtttcaa aacagagcct tgaatagtaa caacaaattt ctcaacaaat tctgattggt
    24781 caaataattc aactttatca tttttttgta ataataaatt atccgcagaa cttgggtcta
    24841 ataatatatt agataaagaa aatttagtaa ttacagtttg ttgattaggc atcactcgct
    24901 taatatatgc tctatctgta taggcactta attttattcc tccagcagca ataattaaat
    24961 cagatatacg ataaccgtct tttaattgat atcttccggg ataacgaact gaacctgaaa
    25021 tacttacacc attctcaatt tcgcctgaaa tatatccaat attaacaaca tcaccgtcga
    25081 acaaaacaaa attttgattt gatgctaata atttctcata atctaaatta ataatttctg
    25141 ttttgtcttt tgccaaacga gtaattaaaa tattctttat aaatgcattg ggttttaaac
    25201 ctccagcaaa tgcaattaat tcatttaatc cttcgccttt tttcaattcg tataaagaag
    25261 ctcttctaac cgcaccttca attttaataa tcttttctgc cactggaaca taaataaaat
    25321 caccattttg aagatagaaa ctttcttcag gaattggatt cattaagaat tgatgaacat
    25381 caaaacgctt aactacttta ccatctcttc ggatttgaat atcacgtacc gtaccaatat
    25441 tagttacacc atccgctgca ttaattgcat tataaactga attaatagct ggaatcttga
    25501 aagtacctgg tgttacaact tcgcctacaa tattcacatc aatagagcgt gaataattta
    25561 atgtaatttc atatttagta ttttgtgtat tgataaagct atttaatctt ttaccaatta
    25621 atgctttaac cgcaccaaaa gttaagcctt taacatataa acgaccaaat tcagtttctt
    25681 gaatatagcc atcatcattt acttttaatt tgttattata atcagcataa ccccaaactg
    25741 aaattaatat ttcatcgcca ggtcctacaa tatatgaatc aggagcttta gtatccgttg
    25801 atttaaagaa caattctaaa ttatttgttg taaagaaacg gtgtccaaaa atttcatttg
    25861 ataagtcaat atctttcaat ttttgctttt caacttttaa actatccaaa attaaaaaca
    25921 tagaatcttg tcttaattta gtttgtaata ctaattgacc atattcaggg tttaataagg
    25981 aactcattaa agcatcattt ggattagaaa cagctgctga tgcatcttcc ccaccgccta
    26041 gcaataactt aataatattc ggatctacac ccatcatttt agcttgctgt aatgatgctg
    26101 cagtatttgc agatgttgta ccaacttttg tagtagctgc agttttggct gtatttaata
    26161 ctgagttata accttgcata cccatattga gtaattgact aagaccagcc atttgagcat
    26221 gagcatttga aatgctaata gatgatataa tgagtaaaac taataaacgg acatttctaa
    26281 aattcatatt gaaatttaat agattttctt taagatacac aaagatataa aaaaaggcta
    26341 tacttatata tagccttttt tttttaagat ttttttacca taaattattt aggtacataa
    26401 ggaaaaaact ctactcttct attcaactga tgttctggtt ctgtttttgc tccagggaac
    26461 atatttacat gctcaccaaa atactcaatt acaaaacgac ttctgtcaat tccaaattta
    26521 ttcactaaat aatcaaccga agcatcaact ctattttgag ataatttatc attatatgga
    26581 tcactagctc taacatcagt atgtcctttt gcaacgactt taacttcagg atacttcttc
    26641 attatatttg caattttata caattcagga atttcagaaa ctctaatatt atacttatct
    26701 aaatcgaaga aaattaatga aagattccaa cctccaacag agatattttt aatttcatta
    26761 ttgataatat tcttaacttc ttgtatttgc tcaggagtta atccatctgg tctaatattt
    26821 acaccatctt tgataggcaa cattggagta gaaaatggtt ctgggtctgt atggtcagga
    26881 taaccatctt tatcactatc caaagttctt ccgtgtgtat ctactattgc accttcaggt
    26941 gtattcggtt cccaatctaa ttcatctatc acaccatcat tatcagcatc tttaactttg
    27001 tattgatttt ttaatttttc atcaactaca ttcatatatg cttctaatgg gtttacccat
    27061 tcactatgtt ctttttgagc gccaatcttt ccaaatttaa acgtaaagtt ggcattcaag
    27121 taagaataag catctctatt cattttagaa agagaaactt tatcaggagc accagtaatt
    27181 aaatccgtta aatctgaacg agaactaaac ttaaatgcat ctaacttatc tgagttagca
    27241 aaaataaaac gccattctac tccaaaatca acaactggtg tacattttat ttttaaacct
    27301 aatgccatag gaaaaactat ttcagtaaat ttatttgtaa aaccttttaa atatctactg
    27361 ttattaattg gtttatcatt tgcaacatta tagatatttg attctaaacg catcataccc
    27421 ataccgattt tagaaaaaac cgaaactctt ctttctttcc aaggtttttg atcaatatta
    27481 gattttatcc atctatttaa gccaaaaaac atattactcc aattgatata aacattagct
    27541 gttgcttgat gaaacacgtt agttttgaaa tatacttctt ggtcaaaacc caatacttgc
    27601 caatagtatc tatcttgagc aaaaccacca cgagttactg ttcttcctga taatttacct
    27661 aatgtataat caatattaat tccaaaaaca tttcctatca ttttagttac gccaagacca
    27721 gcaccccatt gtacttcact cggtgcttta aattgtctta cccaatcata acttctaatg
    27781 tctgtaaaag gagaagtcgc acctccatgg atagtaaatg accagtcttt caaaaattta
    27841 ttcgtttcaa ttctagaaga agttttaata gacgattttg aagtttcctt ttgttcagca
    27901 gaaagcacta catttgcaga aagtaaaaat aaaaaagcta aaataacatt tggagttttt
    27961 cccataaaaa gaatttttag cgaagttaaa caattattta atttaattta acaatcttag
    28021 acttaattta acaataaaat gacaaaattt caaaatgata cgattgtagc cataataaca
    28081 gcaactggtt ctgcagccat aggtgttatc agactatctg gtaatcaagc tattagtata
    28141 ataaactcag tatttaaagg tgttgattta gaaaagcaag catcgcatac cattcatttt
    28201 ggatacatta aaaatgaagc aacaatttta gatgaggttt tggtatcaat ttttaaagca
    28261 ccaagttcct atacaaaaga aaatgttgtt gaaatatctt gtcatggctc aaattatatt
    28321 ttacaacaag tatttatgct tttagtaagc aaaggtgcta gagctgcgat gcctggggaa
    28381 tttacgcaaa gagcctattt aaatggtcgt tttgatttag cacaagcaga agcagttgct
    28441 gatattattg cttcagattc tgaagcagca cataaaattg ctatgcaaca aatgcgaggt
    28501 ggaatttcaa aaaaactaaa agcattaaga acttcattag tagaatttgc ggctcttatc
    28561 gaattagaac ttgattttgc ggaagaagat gttgaatttg cagatagaac taaattcaat
    28621 aacctactac atcaaatatt attagaaatt caagtattat cgtcatcttt taaatatggg
    28681 aacgctatta aaaaaggtat aaaattagct ataattgggc aaccaaatgc tggtaaatct
    28741 agttggataa atgctttaat gaatgatgat attgctattg tttctgatat tgctggcaca
    28801 acaagagata aaattgaaac aactttagat attcaaggag ttctatttag attgattgat
    28861 actgcaggta tccgtgaaac ggaagacacc attgaaaaaa ttggtgtaca acgtgcattg
    28921 gaaacgattg aaaatgctga catagtaatg tatatttttg acatcatcaa tttaaccatc
    28981 gaagatattc aaaaagacct taaactaatc cacgaaaaaa ctaatgctcc agttttaatt
    29041 attgctaata aagctgattt aattacagaa caaaacatta ctctaaaaga ccattttaaa
    29101 catttagaaa acctaactta tttatcagca aaaagcaata ttgatattga aaaagcaaaa
    29161 gacagcatat ataatttggc tttagaaaac tatcatttta atagtgagaa tgtaattatt
    29221 tcaaataata gacattatga agctttaaag aatgcagaag ctgcaattct tgttatttta
    29281 aaacaattag aacaaggaat ttctactgat tttatagcac aagatatcaa aactgcctta
    29341 cgttatcttg gcgacatcac tggaaatatt gatgtagata aagatatact tgggacaatt
    29401 ttttctaagt tttgtattgg caaataagtt actattttgt ataaattaca aaatcaattg
    29461 tccgaattac aaaaattata tataatttta tttatagtaa attgtgcaaa aacaaacaat
    29521 taaatttgaa ccataatcca aaaattatag ttcattgttt ttaaattttt gtcaaatagt
    29581 tgtgatacaa ttgaagtttt aggctacttt gagatgtttt tgcaaataaa aaatattaat
    29641 taagtaccct ttcgggtata tttttacaag gccaaaatta attataccca atagggtata
    29701 attaaaatat ttttactatt tttatacctc taaaggtata aaaatgaaaa cttcattttc
    29761 tgaatttgta aaggaaaagc gtaagcaact taaactgact cagcctgaat tggcagaacg
    29821 ttcaggtgtt ggtcttcgtt ttattcgtga gttagaacag ggcaaagtat ctttaagatt
    29881 agataaagta aatcaagtat tagccttatt tggtacgcaa gttggtgtaa taaaaaataa
    29941 tgaattatga gaaaaggaaa cgtttattac aaaaataatt ttgcaggaat aatagctgaa
    30001 attgaagatg gctatacctt tcagtatgat gaacaatttt taaattcaac ttttgtaaaa
    30061 tctattagcc taacattgcc cattcaaaag cagccttttc atagcaaaat tttatttcct
    30121 ttttttgatg gcttaatacc agaaggttgg ttattaaata ttgctgttgc caactggaaa
    30181 ataaatccac gagataggtt tggtttattg ctaactctat gtaatgattg tattggagct
    30241 atttcaatta aagctatcta gaatgaaatc gaataattgt ctatattgct ataattcttt
    30301 aaatgaagat gaacaagact ttcattcatc ttgtgcaaag aaaatatttg gcacaactgt
    30361 acctcctatc atcgagttta attttaaaca actagaagaa ttagccaagc aaattattat
    30421 caaaagtatt gctgt
//
LOCUS       DAOQCW010000015        30319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_557_length_30319_cov_5.500264, whole genome shotgun sequence.
ACCESSION   DAOQCW010000015 DAOQCW010000000
VERSION     DAOQCW010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 30319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30319
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_557_length_30319_cov_5.500264"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="PLS16_02500"
     CDS             complement(<1..785)
                     /locus_tag="PLS16_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNJ59977.1"
                     /translation="MLDSKIPQGDIDKKWTEYKGHVKLVNPANKRKLEVIVVGTGLAG
                     ASAAATLGELGYRVKTFCFQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTVKG
                     GDYRSREANVHRLAEVSANIIDQCVAQGVPFAREYGGYLDNRSFGGVQVKRTFYAAGQ
                     TGQQLLLGAYSALERQVALGNVKQYTRHEMLEVVIIDGKARGIITRDLVSGKLERFFG
                     HAVVLATGGYGNVFYLSTNAMGSNVTAAWKAHKKGAFFANPCYT"
     gene            complement(830..1513)
                     /locus_tag="PLS16_02505"
     CDS             complement(830..1513)
                     /locus_tag="PLS16_02505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HNJ59978.1"
                     /translation="MATKNFFTSSVGRKIIVGLTGLFLISFLVVHLLINSFILVDLVN
                     PNDNGATFNEAAHFMSHNWLIRVVEIGLFAGLIAHIAMTVKLTIENKKKRPVQYAVFD
                     GKANSKWYARSMAILGSLLLMFLAVHLSQFWWPTKEALYITHADENSFEMVKATLTNP
                     INLAIYVVGVVALGYHLLHGFQSAFQSLGLNHKKYTPIIKTIGTVFAIAVPAGFAFIA
                     LSVFLGLVQ"
     gene            complement(1647..1910)
                     /locus_tag="PLS16_02510"
     CDS             complement(1647..1910)
                     /locus_tag="PLS16_02510"
                     /inference="COORDINATES: protein motif:HMM:NF013083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HNJ59979.1"
                     /translation="MSLQEKFQQAQIDVKTLTKRPTDEQLLNLYALFKQATDGDNTTS
                     KPGMFDIKGQFKWKTWNEKKGMSKDEAMQNYIDLVTKLLDTLK"
     gene            1950..3479
                     /locus_tag="PLS16_02515"
     CDS             1950..3479
                     /locus_tag="PLS16_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59980.1"
                     /translation="MFSKKFTIALFFSLPLFWLFFILTPYYLLPSDCFLDNFVYVDNL
                     IQHHLFQFHHQVALNFDDTDNFIYIFLLYGVKEFLNYSLYKAGIIINGISLFLSFVFL
                     ARTISSRHSNVNFFIMLTLFLSVQIWAALMGDGILFQGLLYIMIARAFWHKRYIGILI
                     WFVVNTINNPINSLYLFPLIIASYSDILELKERERRRFLLLRLRKTVFWLIIPIGIWL
                     IYRKLYFGNFIPNLDFHYSIEKDSKILFMSKSALYMCLHYIKYFILPLFIGVLFYFIK
                     QGKQLHIKHYAVLLSYIILPIIITSLHVQDQNTAYKNYYIIYLGLLILSFIFIRDFRS
                     ITQKTAIGIFIIFYSFNQIADSFIKFSQSYNNNTFRIANDLTHIRKGKIIVYYDNYIS
                     YLTNWESIYANGRHQPKSNRKVLSEDEILALNPDIIIPAGIQIQSSQYSTFKVPENTR
                     QYSLETKPENSLDQIFYEIRKAYNLNKFQYFTILVNNEKENAAYIKKVLIDNGSKELK
                     E"
     gene            3573..5105
                     /gene="lysS"
                     /locus_tag="PLS16_02520"
     CDS             3573..5105
                     /gene="lysS"
                     /locus_tag="PLS16_02520"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNJ59981.1"
                     /translation="MQQLSEQEIIRREKLAKIKELGIDPYPAPLFPVNVTSVEIKNNY
                     QEEVLEDGTKNRLNYQEVCLAGRLMAARPGGKATFAELQDTAGRIQIYVRRDDICPTE
                     DKTMYDIVFAKLLDLGDFIGIKGYAFKTKVGEVSVHVTELTLLAKSLRPLPVVKTDKD
                     GTIFDAFSDPEMRFRQRYVDLVVNTQVKETFIKRTKLMNSIREYLNDKGALEVDTPVL
                     QSIPGGAAAKPFITHHNALDIPLYLRIANELYLKRLVVGGFDWVYEFSRNFRNEGMDR
                     THNPEFTVLEFYVAYKDYFWMMDTTEQLLEKACIDVNGTTEITVGEKAISMKAPFKRI
                     SIYDAIKEHTGIDVSQMNEEELRAVCKQLHIEVDNSMGKGKLIDEIFGEKCEKNYIQP
                     TFIIDYPIEMSPLTKKHRSKEGLVERFELMINGKEIANAYSELNDPIDQRERFEDQAK
                     LLERGDDEAMFIDQDFLRALEYGMPPTSGIGFGIDRLAMLLTNNESIQEVLFFPQMRN
                     EV"
     gene            5179..5742
                     /locus_tag="PLS16_02525"
     CDS             5179..5742
                     /locus_tag="PLS16_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59982.1"
                     /translation="MKTVLFTAPIETENEVMLLIELLELGLDYLYIYKPALDDFSLVD
                     FVEKIPEKYWKQCISNSLIITKEFDFAGYYFTPDILQKNELYNQKIFDWLKQKNKICA
                     TAIAQPNAIANPNQFQHIFVPQSNIKHSRIHQLIALDCSTISDIQQLKATPIKGIEIS
                     NFIWNNEDSEEAKTKMLEILMELNTKK"
     gene            5839..7479
                     /locus_tag="PLS16_02530"
     CDS             5839..7479
                     /locus_tag="PLS16_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HNJ59983.1"
                     /translation="MSEASSNNFIEEIISNDLKEGKYKSILTRFPPEPNGFLHMGHAA
                     SICLNFGVAEKFGGKTNLRFDDTNPITEDTIFVDSIKEDVQWLGFKWENIFYASDYFE
                     QLYQFAIQLIQQGDAYICDLSAEEMAKQKGSLTEPGQNSPYRNRSVDENLTLFEQMRA
                     GKFKEGEKTLRAKIDMASPNMIMRDPLLYRIKFAHHHRTGDKWCIYPMYDFAHGQSDS
                     IENITHSICTLEFVPHRELYDWCIQKLNIYPSHQYEFARLNVNYTVMSKRKLKQLVTE
                     SLVNGWDDPRMPTISGMRRRGITPNSIKTFVQKAGIAKREKVNEFSLLEFCVREDLNK
                     IALRRMVVLDPIKVIIDNLDTEIEVEIENNPEDEYGGNRKLLFTKEIYIEQEDFMENP
                     PKKFFRMSVGQHVRLKGAFIVQCTSIDKDMEGNITQIHCAYLPNSKSGNDTSGVKPQG
                     TLHWVSCQYAIPVEIREYDRLFKVENPDSEEGNFKDYINENSLKTYSNAFAEPALKEA
                     SLNDHFQFLRKGYFYLDKDANDEKIIFNKTVGLKDSWK"
     gene            7515..8756
                     /locus_tag="PLS16_02535"
     CDS             7515..8756
                     /locus_tag="PLS16_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59984.1"
                     /translation="MSQITLQSTTKNTQGTITLNGSKSISNRLLIIDALCNNQINYSN
                     LSNADDTIYLKSILQSNSNLLDAGAGGTTYRFLTAYLATQEGRDVVLTGSERMQQRPI
                     KILVDALKQLGADITYENNEGFPPLRIKGKKLKGGQLTLPANISSQYITALLLIAPTL
                     ELGLELHLEGTIVSIPYIEMTLKMMNYFGIKTSFSIENKIIKVETGNYTPKDFFVEGD
                     WSAASYYYSIAILSDQTQIQLNGLTNDKIQGDAAIAEIAKNFGIHTAYQNQSVVLTKD
                     IKPQLLNFFEYDFLKCPDLAQTVVAFCAALGIDAKFHGLQTLRIKETDRIQALNDELF
                     NLGLAELVEIDNNTWELQNCMPQVYNDYAVQTYEDHRMAMAYAPLALKLGKIKIDEPE
                     VVSKSYPNFWRDIQNIGFESI"
     gene            8915..10198
                     /locus_tag="PLS16_02540"
     CDS             8915..10198
                     /locus_tag="PLS16_02540"
                     /inference="COORDINATES: protein motif:HMM:NF021427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HNJ59985.1"
                     /translation="MNEIICPNCHKAFKVDEAGYADILKQVRDHKFEEELQNRLNIAE
                     REKISAVKLAEANIKNEFQNLISNRDKEITELKANNERTLTEQLAKKDNQITELKAKI
                     ENSKTEMELSVSLAVQKVEKERDELIHTIKLKDTEKELLEQSLNQKYLQDLKAKEEII
                     RIKDQEIEFRKDMKLKFSTKMLGETLEKHCEIEFNKLRASAFQKAYFEKDNDSRSGSK
                     GDYIFKEFDDFGNEIISIMFEMKNEGDETATKKKNEDFFKELDKDRVEKKCEYAVLVS
                     LLEIDNELYNVGIVDVSYKYKKMYVIRPQYFIPMITLLRNAALNSLSLRNELAVIKNQ
                     NIDITNFENSLFEFKEKFAKNYDLASRQFKVAIDEIDKTIDHLQKVKDNLLNSVNNLR
                     LANNKAEDLTIKKLTKGNPTMTAKFEELNKDKPLQ"
     gene            complement(10360..11130)
                     /locus_tag="PLS16_02545"
     CDS             complement(10360..11130)
                     /locus_tag="PLS16_02545"
                     /inference="COORDINATES: protein motif:HMM:NF020448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HNJ59986.1"
                     /translation="MNKQMTYHILNGDCLADQLKQTNIQGQFIICAECLVDGDLTGDT
                     LSQFWTTRAKFISFTYNTAMDDYYNKCVNELEKIINLPDTSEVCLWFENDLFCQTNMW
                     FILSLLSKKQVKKIFRVFPIIDSNIDLWKGFGISNTQMLEQAYNSKIPFTSKDIKLGD
                     YLWNAYKISDFKKLKELSRQESYCFQHLDEVCQAHIDRFPTDNSLGRPDKIVKEIIEN
                     KSEDFQVVFSNFSERQGIYGFGDLQVKNIYDRQFSGTK"
     gene            complement(11258..12841)
                     /locus_tag="PLS16_02550"
     CDS             complement(11258..12841)
                     /locus_tag="PLS16_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HNJ59987.1"
                     /translation="MSNIEQFKEEVTQGYTFKGDSFIVGGGMIDKSAISGLQVKIPLK
                     TLNRHGLIAGATGTGKTKSIQLISEQLSDKGISVLMMDIKGDFSGIAEAGVENQIVKD
                     RVNLIGNSWNATGFPVELMTISNQDGVRLRSTTLEFGPILISKILELNDTQQSVMSIV
                     FKFADENNLPLVDLKDLKKTLQFMAGDGKSEVEQEYGSISPATLGTITRKIVELEQQG
                     AELFFGEPSFQVNDLLRKDENGKGFVNIIRLTDIQDKPKLFSTFMLSLLAEVYSSFPE
                     RGDADQPELVIFIDEAHLVFDSASKTLLDQIETIIKLIRSKGVGIFFCTQNPNDIPAG
                     VLSQLGLKVQHALRAFTAKDRQAIKLVAQNYPITNYYTVDEDITQLGIGEAFISALNE
                     KGIPTPLVHTLMAPPQSRMDILTEEEMRVNIQKSELYLKYKENLDKESAYEILSNRIE
                     ANSQEVAEKNEKKSEAEQSITSKILGKVSQQIFNTATRELAKSATKGISNKMMKGVAN
                     TATREVSRGLLGAILKAFK"
     gene            complement(12871..14316)
                     /locus_tag="PLS16_02555"
     CDS             complement(12871..14316)
                     /locus_tag="PLS16_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59988.1"
                     /translation="MISKFIKSIFSSNKKDKDMATKVNKPSVANKATVVTEKLKAKTP
                     AKKASATSKNTVIKTTVKKEANKKVDKPIVKKAVEKPKTKLVAKAPAKKASAVSKNTV
                     TKTTVKKEANKKVDKPIVKKTVEKPKTKIVEKAPAKKASAVSKNTVTKTTVKNEANKK
                     VDKPIVKKAVEKPKTKIVEKAPAKKASAVSKNTVTKTTVKKEANKKVDKPIVKKAVEK
                     PKAKSVAKAPAKKTSAVPKNTVTKITVKKEANKKVDKPIAKNETIKASKQTVKLENNK
                     IERAILDDKVSNLKKISKQDMTKETSKPKAGNKPVKVEKSEPPVAPKVVPTPGAKRKV
                     IVDYKNVPEEVLMALSEKYPHGYNRGIIKFTNAKNEVVSAVPIELGDTSYLVKVSTQL
                     QKMVDNVDLDEDDDIVVAGLDKEVPAGATKDMEEFDKADDDEDKPKKTKSKGKVGSAD
                     DLEYGFDYDDDDDDDDDDDDDDDDEDDDDED"
     gene            complement(14445..14918)
                     /locus_tag="PLS16_02560"
     CDS             complement(14445..14918)
                     /locus_tag="PLS16_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59989.1"
                     /translation="MLIKIKDYFKQLQLLHGALTFGVLVILIVFQYVLPKQEHLVNQQ
                     AVYFGVGIAVVCVIVSRIVFVNITTKNRVKEQMKEKLDAYRSAFVIQIGILEGGAIFN
                     AILLYLFGSEINFAMSLALVFLMITRRPTKKDVYRTMYPDNVQNTALDKDDKIII"
     gene            14987..15730
                     /locus_tag="PLS16_02565"
     CDS             14987..15730
                     /locus_tag="PLS16_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59990.1"
                     /translation="MKKILYILIFTLVIVSCKKETETIELSNDYNYFPTKIGSYIIYK
                     GDSILYNYFFSEVKRTKSFYLKEVVSDTSRDNLNRLAYEVDIFESQDLNSTWTHKVKW
                     YKVIDNKTAESVEDNLRYLKLVFPSKVGLTWNANKFISDRIPYIYAIDSTLDIFNSRA
                     VIIQKDVTYTNSFTSFDSTLTVVNLIDSSGVDYYKFTEKYARNIGLVYKEQWNVVAKD
                     PGRNPNLPWIDRARLGFYLKLEAIEYGQE"
     gene            15717..17282
                     /locus_tag="PLS16_02570"
     CDS             15717..17282
                     /locus_tag="PLS16_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNJ59991.1"
                     /translation="MVKNKLILFLILFQLYAFSQNANLVIVEFKDKGVDNNSSIDAPI
                     NDTYIDTISKLNLHFINASKWLNTAIFKYEDIDVIDKIKTFSFVQKLSFGCINEMPNG
                     RLNQEKDKFNAESISEQTEEFDKNYYLDQIIQLKGDFLQEKNLKGQSIKIAVIDNGFP
                     KSNEIEGLQHIYYENRLMATYDYYGNNSNVYFSSSSSHGTKCFSIIGGQLDNFSGSAP
                     KAAFYLYRTEVDAIEGQTEEILMSKALEDAVDAGVQVVNISLGYGDGFNDGTSNHTYE
                     ELNGKGTLAAKAVNIASSKGLLVCISAGNEGNSSWKYLTTPADADSAFTIGAINSYNE
                     IAKFSSINFPNNNNIKPNVVALGVGVSILNETNGVSTGSGTSFASPIIAGLAACLWQA
                     FPNKTNWEIKTAIEQSADKFYNPDKSYGYGLPNFEKAYHLLSLPAFNEGEILKVFPNP
                     SSEILNIYLNNENIQSIYLSNYLGQIIYQQQFNSISTTQNYSFSIKDLPNAFYILQIK
                     TNHSKHSKIILKQ"
     gene            17308..19677
                     /locus_tag="PLS16_02575"
     CDS             17308..19677
                     /locus_tag="PLS16_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNJ59992.1"
                     /translation="MYIFTTLMKANNLLFFFFLIISFSSFAQENYTLSGFVKDAKNGE
                     VIIGANIYLSSDASEGTVSNIYGFYSISVPAGQQKIVVSYIGYQSQEIELDIKSNQKH
                     NFDLKEENNILAEVVITSERKDENVKNTKLGTIELSSDRIKSIPAFMGEVDIIKALQL
                     MPGVQAAGEGNSGIYVRGGGPDQNLVLLDDAIVYNPGHLFGFFSVFNNDAVKNVTLVK
                     GAMPANYGGRLSSVIDVQMKEGNNKKFQMEGGLGLISSRLTLQAPIQKNKSSFLISGR
                     RTYAFDIAQPALKKTDFAGTNYFFYDINLKANYIISDKSRLYISGYFGRDVFVYNSKE
                     NQTNVKIPWGNITSTIRYNHIISPKLFMNVSFIFNDYKFSFTGKQTDFSFKANSGVRD
                     YNVKIDFDYYLSNKHLLKFGYDYTHHRMTPTLASATSDTTEFKTDKISKKYSHEMAWY
                     VQHEWEISKKVKLDMGFRLSFFQHIGPYKEINGMDTIQYKRGKAIQPYVGYEPRLAMR
                     YAINESSSIKTGLSLNYQYIHLVSNSNSTLPTDVWVQSTARVKPQQALQFNLGYFKNF
                     KENMYEMSVEFYYKQLWNQIEYTESYVSELDVDPELSYVFGKGKAYGAEFFIRKNKGK
                     LNGWIGYTISRSDRKFSNLNNGNVFPTKYDRPHDLSVNLAYNLNNEWQFGTTFVYASG
                     NTMTPVIGAFVTEGALGFIYGKRNSYRIPAYHRMDLSLTYTPIPKRKPNRRWQSSYNF
                     SIYNVYSRKNVYFIYNKIKGSIASGDIRVQPIQVSLFPIIPSFTWNFKF"
     gene            19682..20653
                     /locus_tag="PLS16_02580"
     CDS             19682..20653
                     /locus_tag="PLS16_02580"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HNJ59993.1"
                     /translation="MHTLKKILLFCFVIISISCEKEFNMKLKETSNIIAVEGKIENDF
                     PPYILLTNSFSIYKDFNINSLTNLFIQGANISIFHNHDTIHLKEYNSTNIHEMPDSFQ
                     VELAGRLGFDITQFGNNFPPFISIYTIDFSQTPLFVGELGQTYGLEIKVQNKTLTAST
                     SIPNRTAHFDSLWIETHPNPDKFPDYFQLTGTLSDLGSSTDYYRYATKVDNEPWLISN
                     SSVFDDAFFNGKKFKIFIPKGNRFGDIPDPLDETVGYWNNQDSVLTFKLSLIDKPHYD
                     FWRTLESNRSSQGSPFGSFVVVKSNIQGDGIGIWGGYASYTHHFSRF"
     gene            20734..21015
                     /locus_tag="PLS16_02585"
     CDS             20734..21015
                     /locus_tag="PLS16_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNJ59994.1"
                     /translation="MPKKVKIILDGREYNINVNDDETVLDAAIDADLDPPHACRVAAC
                     CSCKAKVMQGKVEMDDDEPLTEEEKEEGFVLTCQSHPITDDVIISYDEV"
     gene            21112..21810
                     /gene="pdxH"
                     /locus_tag="PLS16_02590"
     CDS             21112..21810
                     /gene="pdxH"
                     /locus_tag="PLS16_02590"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HNJ59995.1"
                     /translation="MKFQLCSTYSKNINLENQMHNKDLSDFRNNYKLGSLAIDDCKND
                     PNEQFQDWLKDAIDAQLYEPNAMQIATVGKDQQPSLRTVLLKEVIDNGFVFYTNYNSK
                     KGQQLSENNYASLLFWWREQERQVRIEGIVEKFDRNESEKYFYSRPHESQIGALTSPQ
                     SKVIPNRAFLDNRYDELAQEFENKTVPFPDYWGGYILIPNLFEFWQGRESRLHDRIEY
                     ILKENSWTKQRLAP"
     gene            21925..22785
                     /locus_tag="PLS16_02595"
     CDS             21925..22785
                     /locus_tag="PLS16_02595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ59996.1"
                     /translation="MLKEKNILITGANSGIGRVTATELAKLGTNVIMVCRNKDKAQAV
                     QEDINALTGLNNVDLFIADFSSPTSIQAFAEKFYKKYNTLDVLINNAGGIFSERTVNE
                     SNIELTMATNHLGYFLTTHYMLPALKQAPNARIVNVASMAHRLVSFNYNNLNGESNYN
                     FFKQYSISKLCNVLFNKQLASILRNTSKITSNCLHPGNIASNFGKSSVPAFNFLTQQF
                     SFMLSSPQKGAITSIYLASSPFVQDKTGLYWYKKNPVLPSLDAINSEYAKQLWEWSLE
                     KTSIKEFGKF"
     gene            complement(22875..23015)
                     /locus_tag="PLS16_02600"
     CDS             complement(22875..23015)
                     /locus_tag="PLS16_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59997.1"
                     /translation="MEYLVYLIMVIMGIVGNANKTTPVGGTDKPSDVPTTAKIIIIDT
                     NE"
     gene            23183..24673
                     /locus_tag="PLS16_02605"
     CDS             23183..24673
                     /locus_tag="PLS16_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ59998.1"
                     /translation="MNNLKDIIFIINKRKLSQIDIFDKTLISCKDSLFSKFYQGLADG
                     KFNSDDEAAHYLYGTDKTDQRFRQLKHRFKKRLLNTLYFIDINTNPSTSVFDKAYYEC
                     INRLHICNIVQKYGGLRATTTFLINETYPTAEKHQFVDVLTEFSYKLLIQHALIGNEK
                     QFRAELKKYNTYTKQLLILRESSLINYESALIFQKPKFGNRKEDINLIQKKIERLFNL
                     YQDFPSMITFFNYTWVKLQFFDYTANNKLLLETSNDFLENYYKYKASGNISTYLVGVN
                     IYKTKALLSSRKFELTYQSANTIKQKTIGTNYFIISEFEFKAAINSTNINLAQKIISE
                     TIKHPQFKSLATNLKERWLIYEAYVILFNNVVNKEPCKIKINKLINQTPEITKDKQGF
                     NLAIKILELLILLYQRKYVEYSDKIETFNTYTYRYLSKNEYKRIKLFTKLLNKLEKTN
                     YNHKQLTNLNEYNLLKDAYLQQTLHESEIVFYDYLWVLILENLKQN"
     gene            24830..26017
                     /gene="mnmA"
                     /locus_tag="PLS16_02610"
     CDS             24830..26017
                     /gene="mnmA"
                     /locus_tag="PLS16_02610"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNJ59999.1"
                     /translation="MKRVIIGLSGGVDSSVAAYLLQQKGYEVIALFMKNWHDTSVTIS
                     DECPWIEDSNDALLVAKKLNIPFNTIDLSTEYKARIVDYMFAEYQAGRTPNPDVLCNR
                     EIKFDIFLKHALALGADFVATGHYCRKASEIIDGKEIYKLLAGKDQNKDQSYFLCQLN
                     QEQLSKALFPIGELTKPEVRKIAKELDLITAEKKDSQGLCFIGKVKLPDFLQQQLDPK
                     KGQVLLVEDSKLEPVPPFNYTNCTNEELASLSKKIDFSKIETKNIGEHNGAHYYTIGQ
                     RKGIGIGGFKQALFVVGTDTIKNIVYLAEGENSNALNRPALFIKNEDIHWIRADKKLS
                     IDGTLQIKARIRYRQALEDACLYQKEEGLYVVFKNLQKGITPGQFCAWYSTDELLGSG
                     VID"
     gene            26097..27449
                     /locus_tag="PLS16_02615"
     CDS             26097..27449
                     /locus_tag="PLS16_02615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNJ60000.1"
                     /translation="MQLKGTYKEIWQISYPIFLGNLAHSINQIVDVAYLGNYSKIALD
                     AVTLSGVFFMNLTFIVSGFARGCQIMIARYSGQKEDAKIGKAFDNLLLLALLLSIGIM
                     AFLFFGADFLIAQFISSKAIQLDIKIYLSALTFSVPFVVLSFCFNSFFAGIGETKIIT
                     YATICMALTNMILAYGLIFGHYGFERMGILGAGISFVVAEIVMCLVYLSYFLYHKYHI
                     KFTTFQFNRLNIYTITAITRLSSPIVLQGIIGMTSWQYFFISIEKLGENELAVSGILK
                     SIFIFLGIPVWSVASTSNTVISNLIGQNKIEHVIPALKRLVIFTMSISFLLNLIILIF
                     PYKIISLFTDDQSLLSGILPPFYTMMIGLMFFSSAMIMNQGIIGTGNTKTPALVELFC
                     AIFYVAYCYYFIILKKSSLQIAWGCEVVYWLSLLTYTSIYWGSGIWRKHIKSVEEPII
                     "
     gene            complement(27459..28040)
                     /locus_tag="PLS16_02620"
     CDS             complement(27459..28040)
                     /locus_tag="PLS16_02620"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNJ60001.1"
                     /translation="MVKLAILLSGGGSNAEKICAYFKDNSQVQVKYLLSNKENSGAKR
                     IGETYGIPFRIFNREEFNSGLIADFLLDEGIDVVILAGFLWLVPQNLLATFPDKVINI
                     HPALLPKYGGKGMHGAYVHQAVYNNKETESGITIHLCNEEFDKGAILFQAKVLLNADD
                     APEIIAKKVLQLEHQYFPKVIENFCRSFEEKIG"
     gene            28115..29227
                     /gene="gmd"
                     /locus_tag="PLS16_02625"
     CDS             28115..29227
                     /gene="gmd"
                     /locus_tag="PLS16_02625"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNJ60002.1"
                     /translation="MKKAIITGITGQDGAYLAELLLKKGYEVHGIKRRTSLFNTDRID
                     HLYQDPHIENVNFKLHFGDLSDSTNLIRIIQDVQPDEIYNLGAMSHVKVSFDTPEYTA
                     NVDGIGSLRLLEAIRLLGLVNKTKLYQASTSELYGKVQEIPQSEKTPFYPRSPYAVAK
                     MYAYWITVNYREAYGIYACNGILFNHESPLRGETFVTRKITRAVSKIALGLQDKLYLG
                     NLNAKRDWGHAKDYVEAMYLILQQAEPDDYVIATGKTTEVREFVRMSFKEVGIELEFK
                     GTGVEEKAYIVSCSNPEYQVEIGKEVVAVNPAYFRPTEVDLLIGDPTKSKTKLGWNPK
                     YDLAELVKDMMTADLQLFKRDKYLIEGGHKIFNYHE"
     gene            29303..30256
                     /locus_tag="PLS16_02630"
     CDS             29303..30256
                     /locus_tag="PLS16_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HNJ60003.1"
                     /translation="MEKSAKIYVAGHNGMVGSAIVRKLKSEGYTNILTASSKELNLIS
                     QEDTNKWFDKNQPEYVFLAAAKVGGIQANNIFRADFLYQNLMIECNVIHAAHIYKVKK
                     LLFLGSTCIYPKMASQPLKEDYLLSGLLEETNEPYAIAKIAGIKLCESYKRQYNDNFI
                     SAMPTNLYGPNDNYDLNNSHVLPALIRKFHEAKINNQKQVVMWGTGSPFREFLHADDL
                     ADALYYLMQNYDGEQFVNCGSGEEITIKDLALLVKDIVGYEGEIIHDTTKPDGTPRKL
                     TDVSKLRSIGWKHKIELRDGITEVYKNFEKDYNQIVEKYQS"
BASE COUNT        10848 a         4485 c         4617 g        10369 t
ORIGIN      
        1 gtataacatg gattagcaaa gaatgctcct tttttatgtg ctttccaagc tgcagtaacg
       61 ttagagccca ttgcgtttgt agataaatag aaaacatttc cgtatccccc agttgctaaa
      121 acaacagcat gaccaaaaaa tctttctaat tttccgctca ctaaatctct tgtgattata
      181 ccacgtgctt tgccatcaat aataaccact tccaacattt catgtcttgt atattgctta
      241 acatttccaa gagcaacttg tctttctaaa gcagaatacg ctcctaataa taattgctgt
      301 cctgtttgtc cagcagcata gaatgtacgc tttacttgca caccaccaaa tgaacggtta
      361 tctaaatagc caccatattc acgagcaaaa ggaacgcctt gagccacgca ttggtcaata
      421 atatttgcag atacttctgc taacctatgt acatttgctt ctcttgaacg ataatcacca
      481 cctttaacgg tatcataaaa taaacgataa actgaatctc catcattttg atagttctta
      541 gcagcattga taccaccttg tgctgcaata gagtgtgcac gtctagggga atcttggaaa
      601 caaaaagttt ttactctata acctaattct cctaaagttg cagcagcaga tgcaccagct
      661 aaacctgttc caactacaat aacttctaat tttcttttat ttgcagggtt aactaattta
      721 acatgacctt tatattccgt ccatttttta tcaatatcac cctgaggtat tttcgaatct
      781 aacatattta ctattttaaa ttttatctta taatctattt aatcaacttt tattgtacta
      841 atcctaagaa aacactcaat gcaataaacg caaatccagc aggaactgca attgcaaata
      901 cagttccaat tgttttaata attggtgtgt attttttgtg gtttaatcct aaagattgga
      961 aggcactttg gaatccatgt aataaatgat aacccaaagc aactactcca actacataaa
     1021 ttgctaaatt aataggattt gttaatgtag cttttaccat ttcaaatgaa ttttcgtcag
     1081 catgtgtaat atacaaagct tcttttgttg gccaccaaaa ttgacttaaa tgtactgcta
     1141 agaacattaa aagtaaagaa cctaaaattg ccattgaacg agcataccat tttgagtttg
     1201 ctttgccatc aaaaacagca tattgaacag gtcttttctt tttgttttct attgttagct
     1261 tcactgtcat tgcaatatga gcgattaagc cagcaaataa gcctatttca acaactctta
     1321 tcaaccaatt gtgagacata aaatgtgctg cttcgttgaa tgtagctcca ttatcatttg
     1381 ggtttactaa atccactaat ataaatgaat ttataagtaa atgaactact aaaaaagaaa
     1441 ttaggaataa acccgtcaga cctactatga tttttcgtcc gacagatgaa gtaaaaaagt
     1501 ttttcgttgc cataaattaa tattttcaat aagtaaacgc aaatttaaca gtttataaaa
     1561 tcaaaataca attaatacaa gttaagtaat tgtatagttt aatttagaat aaatccaaat
     1621 aaaataaggg tttcagcagt atattcctat tttaaagtat ctaagagttt tgtaactaaa
     1681 tcaatataat tttgcatagc ttcatcttta ctcattcctt ttttctcgtt ccaggttttc
     1741 cacttaaatt gaccttttat atcaaacatt cctggttttg aagtagtgtt atcgccatca
     1801 gtcgcttgtt taaataaagc atataaattc aataattgct catcagtagg tcgtttagtt
     1861 aatgttttaa catcaatttg tgcttgttga aatttctctt gtaatgacat aatgtttaat
     1921 tttaatttta aaaagcctaa atttatcaaa tgttttccaa aaagtttaca attgcactct
     1981 ttttttcact accattattt tggttgtttt ttatacttac gccgtattat ctcttaccat
     2041 cagattgttt tttagataat tttgtttacg tagataactt gatacagcat cacttatttc
     2101 aatttcacca tcaagtagct ctcaactttg atgacaccga taattttatt tatatttttt
     2161 tattgtatgg tgtaaaagaa tttcttaact atagtttata caaagcagga ataataatta
     2221 atggaataag tttgtttctg tcatttgtat tcttagcaag aacaataagt agtcgtcatt
     2281 ctaatgtgaa cttttttata atgctcactt tgtttttgtc tgttcaaatt tgggctgcat
     2341 taatgggcga tggtatttta tttcaaggat tattatatat aatgatagct cgtgcttttt
     2401 ggcacaaaag atatattgga attttaattt ggtttgtagt aaatactatc aataatccaa
     2461 tcaatagttt gtatttattt ccattaataa ttgcaagtta ttcagatatt ttagaattaa
     2521 aagaaagaga acggcgaaga ttcctattat taagacttcg taaaacagtt ttttggctaa
     2581 ttataccaat tgggatttgg ttgatatata gaaaattata ttttggaaat tttattccta
     2641 atttagattt ccattatagc attgaaaaag atagcaaaat attatttatg agtaaaagtg
     2701 cattatatat gtgcttacat tacatcaaat attttatttt acctttattt attggcgttt
     2761 tattttattt tattaaacaa ggaaagcaat tacatattaa gcattatgca gttctgttga
     2821 gttatataat tttgccaatt attattacct cactacacgt tcaagatcaa aatacagcat
     2881 ataaaaatta ctatatcatc tatttaggtt tattgatttt aagctttatt ttcattcggg
     2941 attttcgctc aattactcaa aaaacagcta ttggtatttt tattattttt tatagtttta
     3001 atcaaattgc agacagtttt ataaaatttt cgcagagtta caacaataat acatttagaa
     3061 ttgcaaatga tttaactcac attagaaaag gaaaaataat agtttattat gataactata
     3121 tcagctattt aaccaactgg gagagtattt acgccaatgg aagacaccaa cctaaatcaa
     3181 atcgaaaagt actatctgaa gatgaaatcc ttgctctgaa tccagatata attattccag
     3241 caggcattca aattcaatca tctcaatact cgacttttaa agtgcccgaa aatacacgtc
     3301 aatattcctt agaaacgaaa cctgaaaact ctttagacca aatattttac gaaatcagaa
     3361 aagcatacaa cctaaataaa tttcaatatt ttacaatttt agtgaataat gaaaaagaaa
     3421 atgctgctta tatcaaaaaa gtgctaatag ataatggttc aaaggagctg aaagaataat
     3481 tcctattata aatctttatt aacttacaag ctatcctatt aattatagaa atactataag
     3541 taactttact aacaaaataa cgcaatttaa ttatgcaaca attaagtgaa caagaaatca
     3601 tcagaagaga gaaattggca aaaatcaaag aattaggcat tgacccctat cctgccccac
     3661 tttttccagt aaatgtaact tcagttgaaa taaaaaataa ttatcaagaa gaagtattag
     3721 aagatggtac aaaaaataga ttgaattatc aagaggtttg tttggctgga cgtttgatgg
     3781 cagcaagacc tggtggaaaa gcaacttttg ctgaacttca ggatactgca ggtagaattc
     3841 aaatttatgt tagaagagat gatatttgtc caacagaaga caaaacaatg tatgatattg
     3901 tatttgctaa attattggat ttaggcgatt ttattgggat taaaggatat gcattcaaaa
     3961 ctaaagttgg tgaagtttcg gtacatgtaa cagaattaac tcttttagct aaatcattaa
     4021 gaccgcttcc agttgtaaaa acagacaaag acggaactat tttcgatgca ttttcagacc
     4081 cagaaatgcg tttcagacaa agatacgtag atttagtcgt gaatactcaa gtaaaagaaa
     4141 cattcattaa acgtactaaa ttaatgaatt ctattcgtga atacttgaat gacaaaggag
     4201 ctttagaagt agatacacca gttttacaaa gtattccggg tggtgctgcc gcaaaaccat
     4261 ttattacgca ccataatgct ttagatattc ctctatattt aagaattgca aatgaattat
     4321 atctaaaaag attggttgtt ggtggatttg attgggtgta tgagttttcg agaaacttca
     4381 gaaacgaagg catggacaga acacacaatc cagaatttac agtcttggag ttttatgttg
     4441 cctacaaaga ttatttctgg atgatggata ctacggagca attattggaa aaagcttgta
     4501 ttgacgtaaa tggcacaaca gaaattactg ttggcgaaaa agcgattagt atgaaagctc
     4561 catttaaacg tatatcaatt tatgacgcca taaaagaaca tacaggaatt gacgtttcac
     4621 aaatgaatga agaggaattg agagctgtat gtaagcaatt acatatcgaa gtggataata
     4681 gtatgggcaa aggaaaatta attgatgaaa tatttggaga gaaatgtgag aaaaattata
     4741 tccagccaac ttttattatt gattacccaa ttgaaatgtc gccacttacc aaaaaacacc
     4801 gaagcaaaga aggtttggta gagcgttttg aattaatgat aaatggaaaa gaaattgcaa
     4861 atgcctactc agaattaaat gacccaattg accaaagaga aagatttgaa gaccaagcaa
     4921 aattattaga gcgtggtgat gatgaagcaa tgttcataga ccaagatttc ttgcgtgcat
     4981 tagaatatgg tatgcctcca acatcaggaa ttggttttgg aattgataga ttagcgatgc
     5041 ttttaacaaa taatgaatca attcaagaag tgctattctt tcctcaaatg cgaaacgaag
     5101 tgtaattttc ataaacatca taaaaatatt ctttttgaat aatttcggaa ttatattcag
     5161 catttttcta taagcattat gaaaaccgta ctttttactg ctccaattga aaccgaaaat
     5221 gaagtaatgc tcttgattga attattggag cttggtttag attatttata tatatacaaa
     5281 ccagcgttag acgatttttc attagtagat tttgtagaaa aaataccaga aaaatattgg
     5341 aaacaatgca tttcaaattc cttgattatt actaaagaat ttgattttgc tggatactat
     5401 tttacaccag atattttaca aaaaaatgaa ttgtataatc agaaaatttt tgattggtta
     5461 aaacaaaaaa acaaaatttg tgcaacagct attgctcagc caaatgcaat agccaaccca
     5521 aatcaatttc aacatatttt tgtccctcaa tctaatatta aacattcccg aatacatcaa
     5581 cttattgctt tagattgtag caccatcagc gatatacaac aattaaaagc aacaccaatt
     5641 aaaggtattg aaatttcaaa ttttatttgg aataatgaag attcagaaga agcaaaaacg
     5701 aaaatgcttg aaattttgat ggaattaaac actaaaaaat agtttttcat ttttcttttt
     5761 catttattct ctaaaagcaa aagattaatc tgagttttat attagattga ttaaatttgc
     5821 aagattatag aaatacttat gagcgaagca agttccaata attttattga agaaattatc
     5881 agcaatgatt taaaagaagg aaaatacaaa tctattctaa ctcgttttcc accggagcca
     5941 aatggttttt tacacatggg acatgcagct tccatctgtt taaattttgg tgtggcagaa
     6001 aaatttggtg gcaaaacaaa tttaaggttt gatgatacga atcctattac tgaagacact
     6061 atttttgttg atagtatcaa agaagatgta caatggttag gctttaaatg ggaaaatatt
     6121 ttttatgctt ctgattattt cgaacaattg tatcaatttg ccattcaatt aattcaacaa
     6181 ggcgatgcct acatttgtga tttgagtgct gaagaaatgg caaaacaaaa gggttcatta
     6241 acagaacctg gacaaaattc tccttataga aaccgtagtg tagatgaaaa ccttacgctc
     6301 tttgaacaaa tgagagcagg aaaatttaaa gaaggtgaga aaactttacg tgctaaaatc
     6361 gatatggctt ctccaaatat gattatgaga gacccattat tgtatcgaat taaatttgca
     6421 catcatcaca gaactggcga taaatggtgc atctatccaa tgtatgattt tgcacacgga
     6481 caatcagatt ctattgaaaa tattacacat tctatctgta ctttagaatt tgttccacat
     6541 cgtgaattgt atgattggtg tatccaaaaa ttaaacatat acccttctca tcaatatgaa
     6601 tttgctcgct taaatgtgaa ttatactgtg atgagtaaaa gaaaattaaa gcaattagta
     6661 acagaaagct tggtaaatgg ctgggacgac ccacgaatgc caactatttc aggaatgcgt
     6721 cgtagaggaa ttacacctaa ctcaattaaa acttttgttc aaaaagctgg aattgccaaa
     6781 agagaaaaag taaacgaatt ttcattgtta gaattttgtg ttcgagaaga tttgaataaa
     6841 attgctcttc gcagaatggt tgttttagat ccgataaaag ttattatcga caatttagat
     6901 actgaaattg aagttgaaat cgaaaataat cctgaagatg aatatggtgg caacagaaaa
     6961 ttgttgttca ctaaagaaat ctatatcgaa caagaagatt ttatggaaaa tcctccaaaa
     7021 aaattcttta gaatgtctgt tggtcagcat gttcgtttaa aaggtgcttt tattgttcaa
     7081 tgtacttcaa tagataaaga tatggaaggt aatatcacac aaattcattg tgcatattta
     7141 cccaatagca aaagcggcaa tgacacatct ggcgttaaac ctcaaggtac tttacattgg
     7201 gtaagctgtc aatatgctat tcctgttgaa attcgtgagt acgacagatt gtttaaagta
     7261 gaaaatcctg atagcgaaga aggtaatttc aaggattaca tcaacgaaaa tagtttaaaa
     7321 acttattcaa atgcttttgc tgaacctgct ttgaaagagg ctagtttgaa tgaccatttt
     7381 caatttttaa gaaaaggcta tttttattta gataaagatg ccaatgatga gaaaatcata
     7441 tttaataaaa ctgttggatt aaaagatagt tggaaataaa ttagtcaaat aaattctata
     7501 attcgccgaa agcaatgtcg caaattacgt tacaatccac cacaaaaaac acgcaaggta
     7561 caataacttt aaatggttca aaaagcattt ccaaccgttt attaataatt gatgcattat
     7621 gcaataacca aataaattac agcaacttat ccaatgctga tgatactatt tatttaaagt
     7681 caattttaca aagtaattcc aatttattag atgctggagc tggtggaact acctatcgtt
     7741 ttcttacagc ttatttagca actcaagaag gtagagatgt tgtattgaca ggttcagaaa
     7801 gaatgcaaca aagacctatt aaaatattag ttgatgcatt aaaacaactt ggtgctgata
     7861 ttacttatga aaataatgag ggcttccctc ctcttagaat aaaaggtaaa aaactcaaag
     7921 gtggacaatt aacattacca gcaaatataa gtagtcaata catcacagcc ttattattga
     7981 ttgctcctac tttggaacta ggtttagaat tgcacttaga aggcactatt gtttcgattc
     8041 cttatataga aatgactttg aagatgatga attattttgg tataaaaaca tcattttcta
     8101 ttgaaaataa aattataaaa gtagaaactg gcaattatac cccgaaagat ttttttgttg
     8161 aaggcgattg gagtgctgct tcttattatt atagtatcgc tattttatca gaccaaactc
     8221 aaatacaatt aaatggtttg accaacgata aaatacaagg cgatgccgca attgctgaga
     8281 ttgcaaagaa ttttggtatc cataccgcat atcaaaatca atcggttgta ctaactaaag
     8341 atattaagcc tcaactactt aatttctttg agtacgattt tttaaaatgt ccagatttgg
     8401 cacaaacagt tgttgctttt tgtgctgctt taggtattga tgctaaattt catggtttac
     8461 aaacattaag aatcaaagaa actgaccgaa ttcaagcttt aaatgatgaa ttgttcaatt
     8521 taggtttggc agaattggtt gaaatcgata acaacacttg ggaattacaa aattgtatgc
     8581 cacaagtata taatgattat gcggttcaaa cttatgaaga ccatagaatg gctatggctt
     8641 atgcaccttt agctttaaaa ttaggaaaaa ttaaaataga tgaacccgaa gtggttagta
     8701 aatcttatcc aaatttctgg cgagatatcc aaaatattgg gtttgaatcg atttagattg
     8761 tgataattaa aatgactgaa ttcaagtaat atatacaact tttgataaaa ttgaattatc
     8821 atttgaaaga ccgcattata caaattcatt tctttaatgc tgtactgttt tgtatcttta
     8881 tttcaaacta attcacgctt aaattataaa tcttatgaac gaaattattt gtccaaattg
     8941 ccataaagct tttaaagttg atgaagctgg atatgctgat attcttaaac aagtaagaga
     9001 tcataaattt gaagaagaat tacaaaatag actcaatatt gcagaacgtg aaaaaattag
     9061 tgctgtaaaa cttgctgaag ccaatattaa gaacgaattt caaaatctaa tttctaatag
     9121 agataaagaa attactgaac tgaaagctaa taatgaacga acattaacgg agcaattagc
     9181 aaaaaaagac aatcaaataa cagaactaaa agcaaaaatt gaaaattcaa aaactgaaat
     9241 ggaactatct gtttcattgg ctgtccaaaa agtggagaaa gaaagagatg agctaattca
     9301 taccataaaa ttaaaggata ccgaaaaaga acttttagag caatcattaa atcaaaaata
     9361 tttacaagat ttaaaagcaa aagaagaaat tataagaata aaagatcaag aaattgaatt
     9421 tcgtaaagat atgaaactca aattttcaac aaagatgttg ggtgaaacac tcgaaaaaca
     9481 ctgcgaaatt gaatttaata agttgcgagc aagtgcattt cagaaagctt attttgaaaa
     9541 agacaatgat tctagatcag gcagtaaagg tgattatatt tttaaagaat ttgacgactt
     9601 tggaaatgaa attatttcta tcatgtttga aatgaaaaat gaaggagatg aaacggcaac
     9661 taaaaagaaa aatgaagatt tttttaaaga attagataaa gatagagttg aaaaaaaatg
     9721 cgaatatgct gttttagttt ccttattaga aattgacaat gaactgtata atgtaggtat
     9781 tgttgatgtt tcttataagt ataaaaaaat gtatgtaata agacctcaat attttattcc
     9841 aatgattact ttattgcgaa atgctgcatt aaattcactt agtcttagaa atgaattagc
     9901 ggttattaaa aatcaaaata tagacatcac taattttgaa aacagtctat ttgagtttaa
     9961 agaaaaattt gctaaaaatt atgatttagc aagtaggcaa tttaaagtag caattgatga
    10021 aattgataaa accatagatc atttacaaaa agtaaaagat aacctattga attccgtaaa
    10081 taatttaaga ttagcaaata ataaagccga agatttgacc attaaaaaac taaccaaagg
    10141 caatcctact atgactgcaa aatttgaaga attaaacaaa gacaagccat tacaatgatg
    10201 agcaacgatc tactatctat tacccactat cctaaatttt tagtaggatt tgcatctatt
    10261 atagaacaag acactttact tttcaggatt ttcttatata cgttataatg ttagcaacat
    10321 acccttctct ctgtccaatt aattgccaat tgttatgaat tattttgtcc cactgaattg
    10381 tctgtcgtaa atatttttta cttgcaagtc accgaaacca taaatccctt gtctttccga
    10441 aaaattagaa aatactactt gaaagtcctc tgacttattt tctattattt cttttactat
    10501 cttatctgga cgaccaagtg aattgtccgt tggaaatctg tcaatatgag cttggcaaac
    10561 ttcatctaaa tgctgaaagc agtaagattc ttgtcgtgat agctctttta attttttgaa
    10621 gtcagaaatt ttgtatgcat tccataagta atcacctaac ttaatatcct tgctggtaaa
    10681 agggattttg gaattgtatg cttgttcaag catttgagta ttggaaattc caaaaccttt
    10741 ccaaaggtca atattgctgt caatgattgg gaaaactcta aatatttttt ttacctgctt
    10801 cttactaagt agcgaaagaa taaaccacat atttgtctga cagaacaaat cattttcaaa
    10861 ccaaagacaa acttctgaag tgtcaggaag attaatgatt ttttcaagct catttacgca
    10921 cttgttgtag taatcgtcca ttgcggtgtt ataggtaaat gaaatgaatt ttgcacgagt
    10981 tgtccaaaac tgcgataatg tgtcacctgt caaatcccca tctactaagc actcagcaca
    11041 aataataaat tgtccttgaa tatttgtctg ttttagttgg tcagctaaac agtcgccatt
    11101 taaaatatga tatgtcattt gtttattcat ttatgctgta atatttccca tgctctcaaa
    11161 aggtgttatc accttttgcc taagttcctt accttccttt ttatagttat ttataggcag
    11221 gtaaaaacac ctgcctgagg cacagtaata aatagaatta cttaaaagct ttcaaaattg
    11281 cacctaatag tcctcttgat acttctcggg ttgctgtatt ggcaacacct ttcatcattt
    11341 tattggagat acctttagtt gctgattttg ctagttctct agttgcagta ttgaaaattt
    11401 gttgagaaac tttacccaat attttacttg taatagattg ctctgcttct gatttttttt
    11461 catttttttc agcaacttcc tgtgaatttg cttcaattct attacttaat atttcataag
    11521 cactttcttt atctaaattt tccttatatt ttagatacaa ttcagatttc tgaatattta
    11581 ctcgcatctc ttcttcagtc aaaatatcca ttctagattg aggcggagcc atcaatgtat
    11641 gaaccagagg cgttggaata cctttttcat tcaaagctga aataaaagct tctccaatac
    11701 ctaattgtgt aatatcttca tcaacagtat aataattggt tattggataa ttttgtgcaa
    11761 ctaattttat ggcttgtcta tcttttgcag taaaagcacg aagtgcatgt tgtactttca
    11821 aacccaattg cgataaaact ccagctggaa tatcatttgg attttgtgta cagaaaaata
    11881 ttccaacacc ctttgaacgt ataagtttaa ttatcgtttc aatttggtct aataatgttt
    11941 ttgaagcact atcaaatacc aaatgagctt catctataaa aatcactaat tcaggttgat
    12001 cagcatctcc tctttctggg aaggaactat aaacctcagc caataaactc aacataaaag
    12061 ttgaaaataa tttcggctta tcttgaatat ctgtaagacg aattatattt acaaaaccct
    12121 ttccattctc atcttttctt agtaaatcat tgacttgaaa tgaaggctct ccaaaaaata
    12181 attcagcacc ttgttgttct aattctacaa ttttacgagt aatcgtacct aatgttgcgg
    12241 gcgaaatact gccatattct tgttctactt ctgatttccc atctcctgcc ataaactgca
    12301 aagtcttttt caaatctttt aaatctacta atggtaaatt attttcatcg gcaaatttaa
    12361 aaacaataga cattacactt tgttgagtat cattaagttc taaaattttt gaaatcaata
    12421 ttggaccaaa ttccaaagta gtagaacgca atcgcacgcc atcttggtta gaaatagtca
    12481 tcaattcaac gggaaaacct gttgcattcc atgaatttcc aatcaaatta actctatctt
    12541 ttacaatttg attttctaca cctgcttctg caattccaga gaaatcgcct ttaatatcca
    12601 tcatcaaaac agaaattcct ttatctgata attgttcaga aattaattga attgattttg
    12661 ttttaccagt accagttgct ccagcaatta gaccgtgtct gtttaatgtt ttaagtggaa
    12721 tttttacttg taaaccagaa atggctgatt tatcaatcat gccaccgcca acaataaaac
    12781 tatctccttt aaaagtatat ccttgtgtaa cttcttcttt aaactgctct atattagaca
    12841 tttatttaat tttgttgatt gttttagata ctaatcttcg tcgtcatcat cttcatcatc
    12901 atcatcgtcg tcgtcatcat catcatcgtc gtcgtcatca tcataatcaa atccatattc
    12961 taaatcatct gcactaccaa ccttgccttt agattttgtt tttttaggtt tatcctcatc
    13021 atcatctgct ttatcaaatt cttccatatc cttagtagca cctgctggaa cttctttatc
    13081 taacccagct acaacaatat catcatcttc atctaaatcg acattatcca ccattttttg
    13141 taattgtgta cttactttta ctaaatatga tgtatcgcct aattcgattg gaacagcaga
    13201 aacaacttca tttttagcat ttgtaaattt aataattcca cgattgtatc catgtggata
    13261 tttttcagac aaagccatta acacctcttc tggtacattt ttataatcta ctattacttt
    13321 tcttttagct ccaggagttg gtactacttt gggtgcaact ggaggttcgc ttttttctac
    13381 tttaactggc ttatttccag cttttggttt tgatgtttct tttgtcatat cttgtttact
    13441 aatctttttt aagttactta ctttgtcatc gagtattgct ctttcaattt tattattttc
    13501 tagttttaca gtttgttttg aggctttaat agtttcattt ttagcaatgg gcttgtccac
    13561 ttttttattg gcttcttttt ttacagttat ctttgtaact gtatttttag gtacagcact
    13621 tgtctttttt gctggtgctt ttgcaactga tttagctttt ggtttttcaa ctgccttttt
    13681 aacgattggc ttatctacct ttttattggc ttcttttttt acagttgtct tcgtaactgt
    13741 atttttagaa actgcacttg ctttttttgc aggtgctttt tcaactattt tagtttttgg
    13801 tttttcaact gcctttttaa cgattggctt atctaccttt ttattggctt cattttttac
    13861 agttgttttt gtaactgtat ttttggaaac agcgcttgcc ttttttgcag gtgctttttc
    13921 aactatttta gtttttggtt tttctaccgt ttttttaacg attggcttat ctaccttttt
    13981 attagcttct ttttttacag ttgtcttagt aactgtattt ttagaaactg cacttgcctt
    14041 ttttgcagga gcttttgcaa ctagtttagt ttttggtttt tcaactgctt ttttcacgat
    14101 tggcttatct acctttttat tagcttcttt ttttacagtt gtcttgataa ctgtattttt
    14161 agaagttgca ctagcctttt ttgctggtgt ctttgctttt aatttttctg ttaccacagt
    14221 agctttattt gcaacagatg gtttattaac cttagtagcc atatctttat cttttttatt
    14281 tgatgaaaat attgatttaa taaattttga aatcatggct taaatgtata atgaaaaatt
    14341 aaatggtgca aacttatatc atctttttta acaaaaaaaa tattaaatat atttttagca
    14401 aaataaataa gtttatttga aagtcaataa agcgtctatt ttaattaaat gataatttta
    14461 tcatctttat ctaaagcagt attttggaca ttatcaggat acatagttct ataaacatct
    14521 ttctttgttg gtcgtctagt aatcattaaa aaaactaatg ctaaagacat tgcaaaatta
    14581 atttctgagc caaataaata taaaagaatt gcattaaaaa ttgctccgcc ttctaaaata
    14641 ccaatttgaa ttacaaacgc actcctatag gcatctaatt tttctttcat ttgctcctta
    14701 actctgtttt ttgtcgttat gtttacaaaa actattctcg aaacaattac acacacaact
    14761 gcaataccaa cgccaaagta cacagcttgc tgattaacta aatgttcttg ctttggcaat
    14821 acatattgaa agacaattaa tataaccaac acaccaaaag tcaaagcccc atgtaataac
    14881 tgtaattgtt taaaataatc ttttattttt atcaacattt tttatatttt ataaggtaca
    14941 aacatacaat atttagaaaa taaaatgcat ctttatattt caaaagatga aaaagatact
    15001 ttacatatta atttttactt tagttattgt ttcttgcaag aaggaaacag aaactattga
    15061 actatctaat gattacaatt actttccaac taagattggt agttatataa tttataaagg
    15121 agattcaatt ttatataatt atttcttttc tgaagtaaaa agaacaaaaa gcttttactt
    15181 aaaagaagtg gttagcgata catcaagaga taatttaaac cgattagctt atgaagtaga
    15241 tatttttgaa agtcaggatt tgaatagtac ttggacacat aaagtcaaat ggtataaagt
    15301 tattgataat aaaacagcag aaagcgtgga agataattta cggtatctta aattagtttt
    15361 cccttcaaaa gtagggctaa cttggaatgc aaataaattt atttctgata ggattccata
    15421 tatctacgct attgactcta ctttagatat tttcaattct agagcagtaa taatacaaaa
    15481 agatgttact tatactaata gcttcacttc ctttgactcc acacttaccg ttgtcaatct
    15541 aattgattca agtggtgttg attattacaa attcactgag aaatatgcta gaaatattgg
    15601 tttagtatat aaagaacaat ggaatgttgt tgccaaagac ccaggcagaa acccaaattt
    15661 accttggata gatagagcaa gattaggctt ttatttaaaa ttggaagcaa ttgaatatgg
    15721 tcaagaataa attaatttta tttttaatct tatttcaact ttacgccttt tcacaaaatg
    15781 ccaatttagt tatagtagaa tttaaagata aaggtgtaga taataattca tctatcgatg
    15841 caccaattaa tgatacttat attgatacta tcagcaaatt aaatctccat tttatcaatg
    15901 cttctaaatg gttgaatact gccattttca aatatgaaga tattgatgta attgataaaa
    15961 taaaaacatt ttcatttgtt cagaaattaa gttttgggtg tataaatgaa atgcctaatg
    16021 gacgtttaaa tcaagagaaa gataagttta acgcagaaag cataagcgaa caaacagaag
    16081 aatttgataa aaattattat ttagatcaaa tcatacagtt aaaaggtgat ttcttacaag
    16141 aaaaaaactt aaaaggacaa agtattaaaa ttgctgtaat cgataatggg tttccaaagt
    16201 ccaatgaaat tgaaggctta caacatattt attatgagaa tagattaatg gctacttatg
    16261 attattatgg caacaattct aatgtttatt ttagttcatc aagcagccat ggaacaaaat
    16321 gcttcagtat aattggcgga caattggata atttttctgg ctcagctcca aaagcagcat
    16381 tttatttata cagaactgaa gtagatgcaa ttgaaggtca aacagaggaa atattaatga
    16441 gtaaagcact agaagatgca gttgatgctg gtgttcaagt ggtaaatatt tcgcttggct
    16501 atggtgatgg atttaatgat ggaaccagta atcatactta tgaagagtta aatggcaaag
    16561 gcacgctagc tgctaaagcc gtaaacattg catcctcaaa gggtttgtta gtatgtatat
    16621 ctgccggaaa tgagggaaat agcagttgga aatacttaac cacacctgca gatgcggata
    16681 gtgcatttac gattggagct ataaatagtt ataatgaaat tgcaaaattt tctagtataa
    16741 atttccctaa caataataat ataaaaccaa atgttgttgc attgggtgtt ggagtttcaa
    16801 tattaaatga aacgaatgga gtttctacag gcagtggaac ttcattcgct tctccaatca
    16861 ttgcagggtt agcagcttgt ctttggcaag catttccaaa taaaacaaat tgggaaatca
    16921 aaactgcaat tgaacaaagt gctgataaat tttataaccc agacaaatct tatggttatg
    16981 gcttaccaaa ttttgaaaaa gcctatcatt tattaagttt gccagctttc aatgaaggag
    17041 aaatactaaa agtattccct aatccttctt cagaaatatt aaatatctat ctcaataatg
    17101 aaaacattca atcgatttat ctgagtaatt atttaggaca aattatttat caacaacagt
    17161 ttaattcaat aagtacaact caaaactatt ctttttcaat caaagaccta cccaatgcat
    17221 tttatatatt acaaattaaa accaatcatt caaaacattc taaaataatt ttaaaacaat
    17281 aagattatgc attctatcta ataaaaaatg tatattttta cgactttaat gaaagctaat
    17341 aatctacttt tcttcttttt tctaataatt agtttttctt cttttgctca agaaaactat
    17401 acattgagtg gttttgttaa agatgctaaa aatggagagg ttattattgg tgcaaacatc
    17461 tatttaagta gtgatgcttc tgaaggtaca gtttcaaaca tttatgggtt ttattcaatt
    17521 tcagttcctg ctggacaaca gaaaattgtt gtttcctata ttggttatca atcacaagaa
    17581 atcgaattag atatcaaaag caaccaaaaa cacaattttg acttaaaaga agagaataat
    17641 atattagcag aagttgtaat tacttctgaa aggaaagatg aaaatgtaaa aaatacaaaa
    17701 ttaggtacta tcgaactttc atcggataga attaaatcta ttccagcttt tatgggtgaa
    17761 gttgatatta ttaaagcgct tcaattaatg cctggagttc aagctgctgg agaaggaaat
    17821 tcaggtattt atgttcgtgg tggtggacct gaccaaaact tagttttatt agatgatgca
    17881 attgtttaca atcctggaca tttatttggt ttcttttctg tttttaataa tgatgctgtt
    17941 aagaatgtta ctttagtaaa aggtgctatg cctgcaaatt atggcggtcg gctttcttcg
    18001 gtaattgatg ttcaaatgaa agaaggtaac aataaaaagt tccaaatgga aggtggtcta
    18061 ggtttaatta gctcgagatt aacattacaa gcacctatac aaaaaaacaa aagttctttt
    18121 ttaatttcgg gaagaagaac ttatgctttt gatattgctc aacctgcatt gaagaaaaca
    18181 gattttgctg gtactaatta ttttttctat gacataaacc taaaagcaaa ttatattatt
    18241 tctgataaaa gtcgattgta tattagtggt tattttggta gagatgtttt tgtttataat
    18301 tctaaagaaa atcaaacaaa cgtaaaaatt ccatggggaa atatcacttc taccattcga
    18361 tacaatcata tcattagccc aaaattattt atgaatgtgt catttatttt caatgattat
    18421 aaattttcat tcactggaaa acaaacggac ttttctttta aggcaaattc aggtgtccga
    18481 gattacaatg taaaaataga ttttgactat tatttaagca ataaacatct actaaaattt
    18541 ggatacgatt atacacacca tagaatgaca cctaccctag cctctgcaac ttcagatact
    18601 acagaattta agacagacaa aatttcaaag aaatattctc atgaaatggc gtggtatgta
    18661 caacatgaat gggaaattag taaaaaagta aaattagata tgggtttccg tttaagtttc
    18721 tttcaacata taggtcctta caaagaaatt aacggcatgg atacaattca atacaaaaga
    18781 ggcaaagcca tacagcctta tgttggatac gaacctagat tggcaatgcg ttatgcaatt
    18841 aacgagagta gttctataaa aactggacta agtttaaatt atcaatacat tcatttggtt
    18901 tctaattcca atagtacctt acccactgat gtttgggtgc aaagtacagc cagagtaaaa
    18961 ccacaacaag cattacaatt taatcttgga tatttcaaaa attttaaaga aaacatgtat
    19021 gaaatgtctg ttgaatttta ttacaagcaa ctttggaatc aaatagaata tacagaatct
    19081 tatgtttctg aattagatgt tgatcctgaa ttgagttatg tttttggaaa aggcaaagcc
    19141 tatggtgccg agttttttat acgaaagaat aaaggtaaat taaatggttg gattggctat
    19201 acaatttcaa gaagtgatag aaaatttagt aatctaaata atggaaatgt tttcccaaca
    19261 aaatacgacc gccctcatga tttatcagta aacttagctt ataatttgaa taatgaatgg
    19321 caatttggaa ccacttttgt ttatgcatca ggcaatacaa tgactccagt tattggagca
    19381 tttgttacgg aaggtgcact tggtttcatc tatggcaaaa gaaattctta tcgtattcca
    19441 gcatatcaca gaatggattt atctctaacc tatacgccta ttccaaaacg aaaaccaaac
    19501 cgaagatggc aatctagcta caacttctca atttataatg tttatagtcg taagaatgtg
    19561 tatttcattt acaataaaat taaaggctca attgcaagtg gcgatattag agtacaacct
    19621 atacaagtat ctttatttcc aataatacct tcatttacat ggaattttaa attttaatga
    19681 gatgcatact ttaaaaaaaa tacttctttt ttgttttgtt ataatctcta tttcttgtga
    19741 gaaggaattc aatatgaagt taaaagaaac gtcaaacata attgctgttg aaggtaaaat
    19801 tgagaatgat tttccaccat atattttatt gactaacagt ttttctatct ataaagattt
    19861 taatatcaat agtctgacca atttatttat tcaaggtgca aatatttcta tttttcataa
    19921 tcatgacact attcatttaa aagaatacaa tagtacaaat atccatgaaa tgccagattc
    19981 atttcaagtt gaattagcag gaagattggg ttttgatata acacagtttg gcaataactt
    20041 cccgcctttt atttctattt acaccattga tttttctcaa acacctttat tcgttggaga
    20101 attgggtcaa acttatggat tagaaattaa agttcaaaac aaaacattaa ctgcaagtac
    20161 aagcattcca aatagaacag cacattttga tagtttatgg atagaaacac acccaaatcc
    20221 tgataaattt ccagactatt ttcaattaac tggaacgcta tctgacttgg gaagttcaac
    20281 tgattattat cgctatgcaa ccaaggtaga taacgaacct tggttaatta gtaattcatc
    20341 ggtatttgac gatgcttttt tcaatggcaa aaaatttaaa attttcattc caaaaggcaa
    20401 tagatttggc gatataccag acccattaga tgaaacagtt ggctattgga ataaccaaga
    20461 tagtgtttta acttttaaac ttagtttaat agacaaaccg cattatgatt tttggcgaac
    20521 tttagaaagt aatagaagta gtcaaggaag tccatttggt tcttttgtag ttgttaaatc
    20581 caatattcaa ggtgatggta ttggaatttg gggtggatat gctagttata cgcatcattt
    20641 ttcacgattt taacactaga taattaatta ttatcattta caaatagcat tgctaattat
    20701 aaagtttaaa gtattttagc aaaaaaaata aatatgccga aaaaagtaaa aattatatta
    20761 gacggtagag aatataacat caatgtaaac gatgatgaaa ctgtcttaga tgcggcgatt
    20821 gatgcagatt tagatcctcc tcatgcttgt agagttgcgg cttgttgtag ttgcaaagca
    20881 aaggtaatgc aaggcaaagt tgaaatggac gatgacgaac cattaactga agaagaaaaa
    20941 gaagaaggtt ttgttttaac ttgtcaatca catccaatta ctgatgatgt tataattagt
    21001 tatgatgaag tgtaataaca aaataaaaga ttcaagtata gaaattagcc aaaaatagtt
    21061 tcaacatttc aacaaagtaa aataacctgt tttagaaata agttgaaata aatgaaattt
    21121 caactttgtt caacgtactc aaaaaatatc aacttagaaa atcaaatgca taacaaagat
    21181 ttaagtgatt ttagaaataa ttataaacta ggaagtttag caatagatga ttgtaaaaac
    21241 gacccgaatg aacaatttca agattggttg aaagatgcta tcgatgctca actttatgaa
    21301 ccaaatgcta tgcaaatagc cacagttgga aaggaccaac agccttctct acgtactgtt
    21361 ttgctaaaag aggttattga taatggtttt gttttttata ccaattacaa tagcaaaaaa
    21421 ggacaacaat tgtctgaaaa caattacgct tcactcctat tttggtggcg tgaacaagaa
    21481 agacaggtaa gaattgaagg tattgtagaa aaatttgatc gaaatgaatc tgaaaaatat
    21541 ttctattcca gaccacacga aagtcaaata ggcgctttaa cttcaccaca aagtaaggtc
    21601 attcctaacc gagcattttt agacaatcgc tatgatgaat tagcccaaga atttgaaaac
    21661 aaaactgttc cttttcctga ttattggggt ggttatatat taatacccaa tttatttgaa
    21721 ttttggcaag gtagagaaag tcgccttcac gaccgtatag aatatattct taaagaaaat
    21781 agctggacaa aacaaagatt agcaccttaa atagcttaaa atctgaatta aaattaaaaa
    21841 gaactctact tcttatctaa tttatatcga aaaaatgtaa tttgtgtact ttactatttc
    21901 aaattataaa tagaatataa caaaatgctt aaagaaaaaa acatcttaat tactggtgcc
    21961 aactctggaa ttggtagagt tactgcaaca gaattagcca aattgggaac aaacgtaatt
    22021 atggtatgca gaaataaaga taaggcacaa gcagttcagg aagatattaa tgcattaact
    22081 ggattaaata atgtagattt gtttatcgcc gattttagtt ctcccacttc tatacaagct
    22141 tttgcagaaa aattctataa aaagtacaat accttagatg ttttaatcaa taatgctggt
    22201 ggaattttta gtgaacgaac tgtaaatgaa agcaatatag aactaacaat ggcaaccaac
    22261 catttaggct attttctaac gacacattat atgcttcctg ccttaaaaca agcacctaat
    22321 gcacgaattg tgaatgttgc atcaatggct catcgattag ttagttttaa ttataataat
    22381 ctaaatggag aaagtaatta taattttttc aaacaataca gtatatccaa attatgcaat
    22441 gtacttttta acaaacaact ggctagtatt ctaagaaata cttcaaaaat tacttcaaat
    22501 tgtttacatc caggcaatat cgcttccaat tttggaaaaa gcagcgtacc tgcttttaat
    22561 tttttgacac agcaatttag tttcatgcta agttctcctc aaaaaggtgc aattacgagt
    22621 atttacttag caagttcgcc atttgtacaa gataaaactg gtttgtattg gtataaaaag
    22681 aatccagttc taccttcttt agatgctata aactctgaat atgccaaaca attgtgggaa
    22741 tggagtttag aaaaaacttc aataaaagaa tttggtaaat tttaaaggac tagggaaaat
    22801 aaaggttatt agcgaaaact aataagcttt gggtttttat gtataaaaga cattagtcga
    22861 ctaatcttaa acgattactc attagtgtct atgataatta tttttgctgt tgttggaaca
    22921 tcacttggct tatcagttcc acctactgga gttgttttgt tagcgtttcc aacaattccc
    22981 attatcacca tgattaaata aactaaatat tccataatgt tttttttgta aagttaattt
    23041 aattaactcg ataaaaatac tgcataaaac aataaaaaat aacatgctta ttttaataaa
    23101 aaaaataagc tgattgataa aatatcaata attttaagta tttttgagaa atattagttg
    23161 ataaaattag ccattttgtt acatgaataa cttaaaagac atcatcttta tcattaacaa
    23221 gagaaaatta tcgcaaattg atatttttga caaaaccctt atcagttgca aagatagttt
    23281 attttctaaa ttttatcaag gtttagctga tggaaaattt aattctgatg acgaagcagc
    23341 tcattattta tatggaacag ataaaaccga ccaaagattc aggcaattaa aacatcgatt
    23401 taaaaaaaga cttttaaata cattatattt tatagatatt aacacaaatc catctacttc
    23461 agtctttgat aaagcttatt acgaatgtat taatagatta catatctgca atattgtaca
    23521 aaaatatggt ggtttaagag caactacaac attcttaata aatgaaactt atccaacagc
    23581 tgaaaagcat caatttgtag atgtccttac tgagttttca tataaactac ttattcaaca
    23641 tgcattaatt ggtaatgaaa aacaatttag agctgaatta aaaaaataca atacatacac
    23701 taagcaatta cttatcttga gagagagtag tctcattaat tatgaatcgg cattaatttt
    23761 ccaaaaacct aaatttggaa atagaaaaga ggatattaat ctaattcaaa agaaaattga
    23821 aagattgttt aatttgtatc aagattttcc atctatgatt acttttttta attatacttg
    23881 ggtaaaactc caattctttg attatacggc taacaataaa ctcttactag agacttcaaa
    23941 cgatttctta gaaaattact acaaatacaa agcctctggg aatatttcaa cttatttagt
    24001 aggtgtaaat atctataaaa ctaaagcctt attaagtagt cgaaaatttg aacttactta
    24061 tcaaagtgcc aatacaatta agcaaaaaac aatcggtacg aattatttta ttattagtga
    24121 atttgagttt aaagcggcaa tcaatagtac aaatataaat ttagctcaaa aaataatttc
    24181 tgaaacaatc aaacacccac aattcaaatc attagcaact aatttaaaag aaaggtggtt
    24241 aatttatgag gcttatgtaa tcttattcaa caatgttgta aataaagaac cttgcaaaat
    24301 aaaaataaac aaacttatca accaaactcc tgaaattact aaagacaagc aaggatttaa
    24361 tttggcaata aaaatcctag aacttttaat cttattatat caaagaaaat acgtggaata
    24421 ttctgataaa attgaaactt ttaatacata cacgtacaga tatctaagta aaaatgagta
    24481 caaaagaata aaactattta ctaagctttt aaataaatta gagaaaacaa attataacca
    24541 taaacaacta actaatctca atgaatacaa tctattaaaa gatgcttatc ttcaacaaac
    24601 attacatgaa agtgagattg ttttctacga ttatttatgg gttttgattt tggaaaattt
    24661 aaaacaaaat taaatgttat aaaaaaaaac ggatatgtaa aaaaacatat ccgccgacat
    24721 aaagtctaaa aacataaaaa cccattacaa atataaatca taatttgata attatcaaat
    24781 ctttttttat taattttaca ttttttaata tttatttgac tgtagtaaga tgaaaagggt
    24841 aataattggt ctttcaggtg gagttgattc aagtgtagct gcttatttat tgcaacagaa
    24901 aggttatgag gttattgcac tttttatgaa aaattggcat gatactagcg taaccattag
    24961 cgatgaatgt ccttggatag aagatagcaa cgatgcatta ttagttgcaa aaaagctcaa
    25021 tattccattc aataccattg atttaagcac agagtataaa gcaagaattg tagattatat
    25081 gtttgcagaa tatcaagctg gaagaactcc aaatcctgat gttttgtgta atcgagaaat
    25141 caaatttgat attttcctaa aacatgcttt agcattaggt gcagattttg ttgcgactgg
    25201 tcattactgt cgtaaagcca gcgaaattat tgatgggaaa gaaatttata aacttttagc
    25261 aggtaaagac caaaataaag accagagcta ttttttatgc cagctcaatc aagaacaatt
    25321 gagtaaagct ctttttccta ttggcgaatt aactaaacct gaggttagaa aaattgcaaa
    25381 agaacttgat ttaattactg cagaaaaaaa ggattctcaa ggtttgtgtt ttataggaaa
    25441 agtaaaatta ccagatttcc tacaacaaca attagatccc aaaaaaggac aagtactttt
    25501 agtcgaagat tcaaaattag aacctgttcc accatttaat tatacgaatt gtacaaatga
    25561 ggaattagct tcattatcaa aaaaaataga tttctctaaa attgaaacta aaaatattgg
    25621 agaacacaat ggagcacatt attacaccat tgggcaaaga aaaggcattg gtattggtgg
    25681 tttcaagcaa gcattatttg ttgttggaac tgatacaatt aaaaatatcg tttatctagc
    25741 agaaggcgaa aatagtaatg cattaaacag acccgcttta ttcataaaaa acgaagatat
    25801 acattggata cgagcggaca aaaaactttc aatagatggt actttgcaaa ttaaagcaag
    25861 aattagatac cgacaagcat tggaagatgc atgtttatac caaaaggaag aaggattata
    25921 tgtggtattt aaaaatttac aaaaaggcat tactccagga caattttgtg cttggtattc
    25981 gactgatgaa ttattgggtt ctggtgtaat tgattaaatt cagaactata ttcattatat
    26041 taatactaca atccatttct gtttagtact tttgtgtctt tttaaattat atttttttgc
    26101 aattaaaagg cacttataaa gaaatttggc aaatctccta ccctattttt ttaggaaatt
    26161 tagcccatag catcaatcaa attgttgatg tagcttattt aggaaattat tcaaaaatag
    26221 cgttagatgc tgttacgtta tcaggtgttt tttttatgaa tctcactttt atcgttagtg
    26281 gatttgctcg tggttgtcaa attatgattg ctcgatattc tggacaaaaa gaagatgcta
    26341 aaattggaaa agcttttgat aatttattgc tattagcttt attattaagt attggaataa
    26401 tggcgttttt attttttggt gcagattttt tgatcgctca atttataagt tctaaagcta
    26461 ttcaactaga tataaaaatc tatttgagtg cattaacgtt ttcagtgcca tttgtagttt
    26521 taagcttttg tttcaatagt ttttttgcgg gtattggcga aacaaaaatc attacttatg
    26581 ccacgatttg tatggctctt acgaatatga ttttagctta tggtttaatt tttggacatt
    26641 atggctttga aagaatgggg attttaggtg cagggatatc atttgtagtc gcagaaatcg
    26701 tgatgtgctt agtttattta agctactttc tatatcataa ataccatatc aaatttacta
    26761 catttcaatt taataggcta aatatctata caattactgc aattacaaga ctatcctctc
    26821 caatagtttt gcaaggaatt attggtatga cttcttggca atattttttt atttctattg
    26881 agaagctggg tgaaaatgaa ttagcagttt cgggaatttt aaaaagtatt tttatctttt
    26941 taggcattcc tgtttggagt gtagcatcta caagtaatac tgtcattagt aatttaattg
    27001 gtcaaaataa aattgaacac gtcatacctg cactaaaaag gctggttatt tttacgatgt
    27061 ccatttcttt tctcctcaac ttaataatac ttatttttcc atataaaata atcagtttat
    27121 ttacagacga ccaaagtctc ctatctggaa ttttacctcc attctatacg atgatgattg
    27181 gattgatgtt tttttcgtct gctatgatta tgaatcaagg aataattggt actggaaaca
    27241 caaaaacgcc tgctcttgtt gaattatttt gtgctatttt ttatgtcgct tattgctatt
    27301 attttatcat cttaaagaaa agtagtttgc aaattgcttg gggctgtgag gttgtatatt
    27361 ggctgagttt attgacctat acctctattt attggggaag tggaatatgg cgtaaacata
    27421 ttaaatctgt tgaagaacca attatctaaa taatattttc accctatttt ttcttcaaaa
    27481 cttcgacaaa aattctctat aacttttggg aaatattgat gctctaattg caaaactttt
    27541 tttgcaatta tttcaggtgc atcatctgca tttaataata ctttagcttg aaataaaata
    27601 gcacctttat caaattcttc attacataaa tgaatagtta taccactttc tgtttcctta
    27661 ttgttatata cagcttgatg tacataagca ccatgcattc ctttgcctcc atattttggc
    27721 aacaatgcag ggtgaatatt aattacttta tcaggaaatg tagctaataa attttgtgga
    27781 actaaccaca aaaagccagc aagaatgacg acatcaatac cttcgtctaa taaaaagtca
    27841 gcaatcaatc cagaattaaa ctcttctcta ttaaaaattc taaatggaat accataagtt
    27901 tctccaattc tttttgcacc tgaattttct ttattactta ataagtattt tacttgtact
    27961 tgtgaattat ccttgaaata tgcacatatt ttttcggcat tactgcctcc accacttaat
    28021 aaaattgcta gtttgaccat tctataaaaa acattcttaa taatttcaaa ataaaggaaa
    28081 ataaccctat ttttgtacgt tttaatatta aattatgaag aaagcgataa ttacaggtat
    28141 tactggtcaa gatggtgctt atttagcaga gcttttattg aaaaaaggct atgaagtaca
    28201 tggaataaaa agaagaacct ctttatttaa cacagatagg attgatcatt tataccaaga
    28261 ccctcatata gaaaatgtaa attttaaatt acattttggc gatttatcgg actctaccaa
    28321 tttaattcga attatacaag atgtacaacc agatgaaatt tataatttag gtgcaatgtc
    28381 gcatgtaaaa gtatcatttg atacacctga atatactgca aatgtagatg gcattggctc
    28441 tttacgttta ttagaagcca taagattatt aggcttagtc aataaaacaa aattatacca
    28501 agcttcgact tctgaattgt atggaaaagt tcaagaaatc ccacagtcag aaaaaacacc
    28561 tttctatcct cgttctcctt atgctgttgc aaaaatgtat gcttattgga ttactgtaaa
    28621 ttacagagaa gcttatggta tttatgcttg taatggaatc ctatttaatc acgaatcacc
    28681 actacgtggc gaaacttttg ttactagaaa aataacaaga gctgtttcca aaattgcatt
    28741 aggtttgcaa gacaaattgt atttaggaaa cttaaatgcc aaaagagact ggggacacgc
    28801 aaaagattac gttgaagcga tgtatttaat tttacaacaa gcagaaccag atgattatgt
    28861 tattgcaact ggcaaaacta ccgaagttag agagtttgta agaatgtcat ttaaagaagt
    28921 tggtattgaa cttgaattta aaggcacagg tgttgaagaa aaagcatata tcgtgtcatg
    28981 ttcaaatcca gaatatcaag tagaaatagg caaagaagta gttgctgtta atccagcata
    29041 tttcagacca actgaagttg atttacttat tggagaccct acaaaatcta aaactaaatt
    29101 aggttggaat ccaaaatacg atttagcgga attggtaaaa gatatgatga cagctgattt
    29161 gcaattattt aaacgtgata aatatttaat tgaaggtggt cataaaatct tcaattacca
    29221 cgaatagtta ttaagtagta agaattgaat ggtaggtcag tagtaattta ttattactag
    29281 attttttact aaaagataaa tcatggaaaa aagtgctaaa atatacgttg ctggacacaa
    29341 tggaatggtg ggttctgcaa ttgtcagaaa attaaaatca gaaggctaca ccaatattct
    29401 tacagcatca tctaaagaat taaacttaat ttctcaagaa gataccaata aatggttcga
    29461 taaaaatcaa cctgaatatg tttttctagc tgctgcaaaa gttggtggta ttcaagcaaa
    29521 caatattttt cgtgctgatt ttttatatca aaacttaatg atagaatgta atgtaatcca
    29581 tgcagctcat atctataaag taaagaagtt attgttttta ggaagcacat gtatttatcc
    29641 taaaatggct tctcaaccat tgaaagaaga ttatttattg agtgggttat tggaagaaac
    29701 aaatgaacct tacgctatcg caaaaattgc tggaataaaa ttatgtgagt cctacaagag
    29761 acaatataac gacaatttta tttctgcaat gcctaccaat ttatatgggc ctaatgacaa
    29821 ttacgatttg aataattcgc atgtattgcc cgcattaatt agaaaattcc atgaagcaaa
    29881 aatcaacaat caaaaacaag ttgtgatgtg gggaactggc tctccttttc gtgaatttct
    29941 acatgccgac gatttagctg atgctttata ttacttgatg caaaactatg atggagagca
    30001 atttgtgaat tgtggtagtg gcgaagaaat tacgataaaa gatttggcat tattagttaa
    30061 agatattgtt ggatatgaag gcgaaataat acatgatact acaaagcctg atggcactcc
    30121 aagaaaactt actgatgttt caaaattaag aagcattggt tggaaacata agattgaact
    30181 tagagatggc attactgaag tttataaaaa tttcgagaaa gattataatc aaattgtaga
    30241 aaaatatcaa tcataattct gatttatttt ttttattttc gttgaatgca aagtaatatc
    30301 acaaccgaaa ccattcagc
//
LOCUS       DAOQCW010000016        29222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_593_length_29222_cov_5.709123, whole genome shotgun sequence.
ACCESSION   DAOQCW010000016 DAOQCW010000000
VERSION     DAOQCW010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 29222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29222
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_593_length_29222_cov_5.709123"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..2468)
                     /gene="lon"
                     /locus_tag="PLS16_02635"
     CDS             complement(63..2468)
                     /gene="lon"
                     /locus_tag="PLS16_02635"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNJ60004.1"
                     /translation="MFENNQMSDMFSDDQVEMIPILSIDEDESIKTEDLPETVPVLPL
                     RNNVLFPDVVIPISVGRDKSIKAVKKAYKDKKFITVLTQKDANNDEPDFKDLYPIGTL
                     ARIIKLINMPDNGTTVILQGVKKCEVVDLIAEEPFMEVSIKFVEDVPLTDENEEKAFI
                     STMKELSEKIIDISPQIPNEAKIVINNIHKVKFLTHFITSNLNIDAKDKQVILEANDL
                     KQRIQQVIRHLQYEMQVQEIKANIHSKTKTDIDKQQREYFLNQQIKAIQEELGGDMYQ
                     EEINRLKEKAKEKVWSESVAKHFEKEIGKLQRINPAAAEYSVILNYLDWMVELPWNEY
                     TKDNFDLKYAKKVLDEDHFGLDKIKDRILEYLAVLKLKGDLKSPILCFVGPPGVGKTS
                     LGKSIARALDRKYIRMALGGLHDESEIRGHRKTYIGALPGRVIQSLKKVQSANPVFIL
                     DEIDKIGKDHRGDPSSALLEVLDPEQNSTFYDNYLEQEFDLSKVLFIATANNIAEIPA
                     PLRDRMEIIQLSGYSVEEKVEIAKKHLVQKQREAHGLKANQIKISPSVLQYIITKYTY
                     ESGVRELDRVLASIMRNIAKSISLEEEYVPNIDTEKVKLVLGKEKYNHDLYTKNLPLG
                     VAIGLAWTPMGGDILFLESTLSKGKGSLTMTGNLGTVMKESATTALTYLQSIASNLKI
                     DTKVFEENNLHIHVPEGAIPKDGPSAGIALFTALASLYSQRKIKPYLALSGEITLRGK
                     VLPVGGIKEKILAAKRAGIKEIILCNQNERDVNEIQAEFIKGLKFHYVDKMQDVLDIA
                     LLK"
     gene            complement(2665..3102)
                     /locus_tag="PLS16_02640"
     CDS             complement(2665..3102)
                     /locus_tag="PLS16_02640"
                     /inference="COORDINATES: protein motif:HMM:NF021634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNJ60005.1"
                     /translation="MIVITDSNIIFSALITPNGTIAHILKAESKLQLFAPSYLFEEVD
                     KHLSKIEELSPLDRKQIKAEIKTYKRLITIIETKDIPKKYYLEAYEIVIDIDFDDVFF
                     VALNRYKHYKIWTLDNELKRGLEQKGYNICITTAEIKKSLYKK"
     gene            complement(3509..4285)
                     /locus_tag="PLS16_02645"
     CDS             complement(3509..4285)
                     /locus_tag="PLS16_02645"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNJ60006.1"
                     /translation="MLASYLKQVPKGLQTTFDTLKDAEISTDNFSFYTSVSSVFSSKI
                     EGEDIELDSYIKYKKFGIEFLPNYTKKIDDLYNAYTFANTNELNKNNISEAHKLLSKH
                     IVNKNWQGKFRIHNMYVSTPDGRIEYVAASPYEVEIDMQKLYNDISILLKIKMSFEEV
                     LFYASFIHLVFVKIHPWNDGNGRSARLIEKWFLAKKLGKKAWFMQSEKLYFEQHQTYY
                     KNIRLLGLEYSELDYNKALPFLLMLPTCLYNKTTKSIVKK"
     gene            4526..5518
                     /locus_tag="PLS16_02650"
     CDS             4526..5518
                     /locus_tag="PLS16_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNJ60007.1"
                     /translation="MKALVLRENGQLQVETVEKPQLGANQALVKISFAALNHRDEWIR
                     QGQYAKIQIPSILGSDGSGIVESVSNESDSNWVGKEVIINPNQGWGDNPKAQAKTYNI
                     LGMPSQGTLAEYVVVDIDRLRQIPHHLNLEQSAALPLGGVTAFNVTFNKAKVKPHQNI
                     LISGVGGGVAQFAFLFSVAAGANVYATSSKADVIEKCKTLGAKGGCSYKDEVALKSMA
                     KEIGGFDAIIDSACGDGMNLLLSLLKPTGSFAFYGATRGLPKDLNMRNIFWNHLQILG
                     STMGSDQDFVNMVGFVEQYKIQPIIDTSFSLDNAVGAFDRMKNGEQFGKIIVKI"
     gene            5538..6539
                     /locus_tag="PLS16_02655"
     CDS             5538..6539
                     /locus_tag="PLS16_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNJ60008.1"
                     /translation="MNIIIPMAGRGTRLRPHTLVTPKPLVPVAGKPIVEWLAEDIIAL
                     CPEKVDKIGFVIGDFGAEVEQNLLKVAERLGAEGVIFHQEQALGTAHAILCAKELLDG
                     KVVVAFADTLFKSTQQLNAEHDGVIFVQQVADPRAYGVVKMDANSRITDFVEKPQEFV
                     SDLAIIGIYYFKDGVNLRAELQYLIDNNITEKGEFQLTNAMENMKNKNKAFYPGKVEE
                     WLDCGNKDATVYTNQRILEIKKAEITAPIGLNNVNSLIIQPCYIGKNVTLEHTIIGPH
                     VSIGDDSKISNAIIKNSIIQNKSNIQNKIIENSMIGSSTALSGNIENLSIGDFSTSN"
     gene            6543..8312
                     /locus_tag="PLS16_02660"
     CDS             6543..8312
                     /locus_tag="PLS16_02660"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ60009.1"
                     /translation="MNKLVLYLCFFVFAFANNPLAFSKEKKAKNKKGASEVVIPKTKK
                     EILAIQSIFIEGIQADLLGNKKDAIAKYNEVIRLDANNDAAYYELAKLYYSIGDLQQT
                     VFNSQKAIKINPNNEYYYIYLAEALGEAGMYAEASKTYESLIKLKPKEYDYYYDWAYM
                     LSQAKKNDEAINVLNLLETKVGVNEDLIMLKQPLWIQQNKVEDAVKDIDKLIALFPRE
                     EMYYLNKAEIYATNNLPLKAISTYENLLKTHPESADALLGLAELYRKENNTTKQQEYL
                     NQLFNNPKIDIDSKIMAIIPMVEKMGSDSTYDESLLKIVSLINKNYPNNMKAIAAKAD
                     VLYNMDKKEEALNEYIKLVHQHADSLPGTIWIHTYILASELQKMDTLIDVTERAIKVA
                     PDDAFAYFYNAIAHQQKKQNQEAAISIKKGLQNIQKSGLSVYNSQLKLQSLIVLGDIS
                     YELKDYTSMDSAYNAALEIDPNNSNTLNNYAYYLSERDVNLVMAERMSKKANLLEDDN
                     SAFMDTYAWIMYKMKNYKEALIWIEDALKLPGANQRSELIEHYGDILIENGRKDDAII
                     QWNKAIELGGDKEVIKNKINKYK"
     gene            8309..9130
                     /locus_tag="PLS16_02665"
     CDS             8309..9130
                     /locus_tag="PLS16_02665"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HNJ60010.1"
                     /translation="MRKLSIFLFAVIIIVACSPTKKAKKSKKKKDDTITTVVDTIKTP
                     EIPKIPSVKDPEIQNILNQIAANELKFTTFYSKVKTKATIDDKTQSFTTQLRWHKYNK
                     IWMSMSIIGIEGARVLINKDSIKIIDRLNSRNILKPISYIQSKAYVNLTYNDIEKLFL
                     AQPILINKDKLELVKGDKENLLKSYDDRFATIIKLDKQNRITNIFITDKLKNQTLNSD
                     YTDYILLGGKYFPKTRNIKIVNGTKNFEMSMDFGDLDISKDLTFPFQINPKYKNE"
     gene            9123..10466
                     /locus_tag="PLS16_02670"
     CDS             9123..10466
                     /locus_tag="PLS16_02670"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNJ60011.1"
                     /translation="MSRILIILSFLLINIFSINAQTNKEQLQKKREQLLKDIKYTQDL
                     LKTKKSDKQASINDLNAINQQINLQQKLIQNSQSQVHLFDNQIIQSKEVIKTKTEELN
                     QLKKEYEQAIINTYKYNKFTDKVLFILNAKSFSESLRRVNYLRRYAIHREKQAEEILQ
                     KSKEIQKDIVVINQKKAEKQTVLNQQMSEKQELGKTQQEKNKIVATLKSEEAKLQSTL
                     SKKQLEAKKLDNQIAAIIKKEIEAAKKAALLAAQKAATEAATKKAAELAAKKAAEKEK
                     TSTSTKTTTTTTKTTTSTTTTPVKPTYTPEYEKLTSSFYGNKGKLPWPVEKGFITKYF
                     GKYNHPDLPGVLIENNGIDIKTDPSSGVRCIFEGTVVGIIDNPVFQNAVIISHGEYFT
                     VYSKLGNVNVSKGQKVNTKQLIGTVYSDIDNVTEVHFEVWKGSEKLNPTTWIYSK"
     gene            10547..11365
                     /locus_tag="PLS16_02675"
     CDS             10547..11365
                     /locus_tag="PLS16_02675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ60012.1"
                     /translation="MKSFNKKVVVITGAGSGIGRALAQNFAKKGAYLAISDLNKEGLA
                     ETVQLCNNTKVYSEIFNVAKKAEFQKFAKNVLDEFGRVDVVINNAGVALGKTKLNDTT
                     YEEFEWLMGINFWGVVYGTKEFLPSLLKQPEAAVVNISSLFGLAGIGEQVPYCSSKFA
                     VRGLTESLRMELLDTNVSVHTVHPGGIKTNIANNARISESEDQEQAKKVLKNFNKALI
                     HTPEKAAQVIIRGIEKKQEKIMIGFETYVTDVVTKVLPTSYSKIFDLLGKQALQ"
     gene            11486..12208
                     /locus_tag="PLS16_02680"
     CDS             11486..12208
                     /locus_tag="PLS16_02680"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793683.1"
                     /note="Catalyzes the transfer of the ammonia group from
                     glutamine to a new carbon-nitrogen group; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HNJ60013.1"
                     /translation="MNVHIIQHEVYEAPGAFLSWIEEKNFTSSFSRVFIDDPLPQSAD
                     NIDILIVMGGPQSPDTSIEQCPHFNAKAEIALIQKCIQNKKIVIGVCLGAQLIGEALG
                     AKYEHSPEKEIGVWPIQLTVDGLKDNNIKHFGTNLPVGHWHNDMPGLTKDCKILAQSI
                     GCPRQIICYSDFVYGFQCHMEFTPEVVDLLINEEEDFLQNNTKHVFVQKPDVIRHQDF
                     SKMNQKLKEFLDNISYKYQQVR"
     gene            complement(12309..14342)
                     /gene="ftsH"
                     /locus_tag="PLS16_02685"
     CDS             complement(12309..14342)
                     /gene="ftsH"
                     /locus_tag="PLS16_02685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNJ60014.1"
                     /translation="MDLLPENEKKKKNSGGGPSGYWIFGLIMAGILAFQFYGLNIKQN
                     DISEEQFFRKVLPSHDVEQVIIVNDKIAEVYIKPDSIKSKDIYKDIRETKLGSENKGP
                     HFIVPIVTVESFKNKFDKAQAELPTEQLVPLKSENRRGLIDIIGWFLPIILFIGVWMF
                     LMRKASGGMPGMGGQIFNIGKSKASLIDNDKDSKVSITFEDVAGLDGAKEEVHEVVDF
                     LKNPQKYTSLGAKIPKGVLLVGPPGTGKTLLAKAVAGEAKVPFFSLSGSDFVEMFVGV
                     GASRVRDLFKQAREKAPCIIFIDEIDAVGRARGKNMMQSNDERENTLNQLLVEMDGFS
                     GDKNIILMAATNRPDVLDAALLRPGRFDRQIYVDLPELKEREAIFKVHLKPLVLDSDV
                     EVPFLAKQTPGFSGADIANICNEAALVAARKDKKAVQKQDFLDAIDRVVGGLEKKNKI
                     IKVAEKRRIAYHEAGHASVSWMTEHANPLIKVTIVPRGQSLGAAWYLPEERSITTKEQ
                     ILDEMCAALGGRAAEEVVFGNISTGALSDLEKVTKQAQAMVKVFGLSDKIGNISYYDS
                     SGQSEYSFQKPYSEKTAELIDKEISETIETQYIRAKQILAENRTKVDQLAKILLEREV
                     IFKDDLELIFGKRPFEEEETTHTEKVQVVNEGITEEEILGEVKAEESIQPSVD"
     gene            complement(14359..14745)
                     /gene="rsfS"
                     /locus_tag="PLS16_02690"
     CDS             complement(14359..14745)
                     /gene="rsfS"
                     /locus_tag="PLS16_02690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNJ60015.1"
                     /translation="METIKKKKTTEKLNKLDLTEVIINLIDDKKGGNIVCMDLRKIPE
                     AITDYFIICDCESTTQTRAITDYVEYEMAKKYGIKAFHVEGRSFGEWCLIDFGDVVVH
                     IFQKERRAFYRLEELWHDAVIKEYKI"
     gene            14783..15544
                     /locus_tag="PLS16_02695"
     CDS             14783..15544
                     /locus_tag="PLS16_02695"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNJ60016.1"
                     /translation="MTTIINDTQYLGKNLILLQSIPSTNSYAKELLTKSKPKNGPVIL
                     AEEQTSGRGQSENIWQSNAKDNLTCSVILETHLIPASKQFLLNMAISLAVLHTTRAFT
                     EEDSTISIKWPNDILVQQKKICGILIENTISGQCLQHSIIGIGLNVNQLDFGMHKQAT
                     SIQQITQKQNDVFLVLKKLLVEMERQLAFIQNEQYLKEQYLKYFFQLNQTAKYIYKEK
                     EVLGIIKNVNEIGQLMIEIDGKIHILNMKEIKFLY"
     gene            complement(15587..16330)
                     /gene="rnc"
                     /locus_tag="PLS16_02700"
     CDS             complement(15587..16330)
                     /gene="rnc"
                     /locus_tag="PLS16_02700"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNJ60017.1"
                     /translation="MFSFIKNIFRKIKTENSVLAEVLKYKDLYQSIGYLPKHSESNYI
                     KALTHASYSKHIQNKNERLEFLGDSVINFVIAEFLYNHFDSKNEGELSKLRSQLVSRT
                     HLNKIGLNLNLQRYIKHKLAPNFYQTSPDILGNTLEALVGAYFLDYTMLGTKPIICKL
                     LLENVDLEQFENEIKDFKSFLYEWVQAEKKQLELKHVANPENDTQFVVDIYIDTIFMA
                     QGKGKNKKEAEKNACLNAYNNLVEQKIIV"
     gene            complement(16333..17595)
                     /gene="fabF"
                     /locus_tag="PLS16_02705"
     CDS             complement(16333..17595)
                     /gene="fabF"
                     /locus_tag="PLS16_02705"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNJ60018.1"
                     /translation="MKFLKPVVITGYGVITPIGNNIQSFTNNLFLGKSGIGSITKFDA
                     SKFRTQFACEVKNYQPEDFFEKKEIKKYDLFTQYALVATREAIKNANLLDYTELNTEK
                     VGVISASGVGGVQTFQQEMKAYYEQANNPRYLSPYFVTKMLIDTVAGYISIHYGFKGI
                     NFSTVSACASSAHAIISAVQNIQLGMADAIIVAGSEAPIHEAGIGSFNALRALSEKND
                     NFENACRPFDKNRDGFVMGEGAGALVIESLEHAQKRGANILAEISGYAMNADAHHITA
                     PSPAGEMIIKNMQQALANAQILNKPIDFINTHATSTPIGDIAEINALKTVFGKDLSSI
                     NISAPKSMTGHLLGAAGVTESIISILAIQYQQVPPTINTKEIDEQIGMDLNLTLGQTQ
                     KRNIKYVLNNNFGFGGHNTSLIFKKFTN"
     gene            complement(17630..17863)
                     /locus_tag="PLS16_02710"
     CDS             complement(17630..17863)
                     /locus_tag="PLS16_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNJ60019.1"
                     /translation="MSEIAERVKKIIVEKLGVEETEITPEANFEMDLKADSLETVELI
                     MEFEKEFNLVIPDEQADKIKTVGEAIAFLESNV"
     gene            18500..19363
                     /locus_tag="PLS16_02715"
     CDS             18500..19363
                     /locus_tag="PLS16_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60020.1"
                     /translation="MNLTKILGVVAITFICITSAFAQKKQLNVGEEFPVAGQGSNQTI
                     VSQNGQYALSKDYSAGNDGRFYTHLVSNPNQKIVEFGAPHYSNTRLKINNGWLNMVGI
                     NPKTNSEPNSVLFIYSNGNGQNTNGGGVKLIVENDGSLSLYSAAGAKVWSKTSPNVKQ
                     EIGRNTLKAGETLKAGERLISSNGAYILRMQEDDGHLCVYKFENGKQGNFVWGTGEHG
                     FKNASLIMQADGNLVVYDGSNVAKWSSKTHPYFDPKFNQAENKPVKLVLENDGSLKLY
                     IASGSVIWTNK"
     gene            19612..20370
                     /locus_tag="PLS16_02720"
     CDS             19612..20370
                     /locus_tag="PLS16_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60021.1"
                     /translation="MAKNFFAEKELKEIDSLIIYNHFEIAEKRTEILSKKLQNNKKYK
                     KHFLETKFRQALILDKKNESPAKQLQLLLKIKDEAETENLFPLMYRIYLLIALSHEKS
                     NNFDLTKKYLDKAFATYKKHRLDSIFSTYCIRNSSYLRFINESDSSFYYAKKANEFAK
                     KYDNEEDLKDSYILLGNFESRKENYLESLKYYYLLLDYIKSLNKYLIGVGYNNISRVY
                     LKMQDIPKALLYNDSAYIYYEEQTLMYRNYFPET"
     gene            20389..21264
                     /locus_tag="PLS16_02725"
     CDS             20389..21264
                     /locus_tag="PLS16_02725"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNJ60022.1"
                     /translation="MGKTDSAFYYFKQYHTDFEALKKEEELVKTKELEEQYQNDKKEA
                     TIKSKQQQILLIIGLLVVIACSLVMLYFKNRKINKQNKIIGEQLIELTKTIEQKEVLL
                     SELQHRVKNNLQHVISILEIQKESVDFNNIDELIRGNQNRIHSMALLHNKLNIADNVA
                     DVNLKQYITELAELVRDSYDNIKQEIHLDIKCKIENISIKKALPLGLIIVELVSNSIK
                     HAFKKQHNRIINIEITKEETMKLYYADNGIGFDFNKLNKKGLGQEIIKGLIDQIDGKI
                     ETKNNNGFELIIYFV"
     gene            21281..21961
                     /locus_tag="PLS16_02730"
     CDS             21281..21961
                     /locus_tag="PLS16_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ60023.1"
                     /translation="MNEKKILLVEDDFLNRRLSKKIFIENNYHVLEAKNTKEALEILK
                     TETVNCAILDINLGKKEADGISLGKQIYENFSIPFIYLTAYETTEIIEKAVATSPYSY
                     LTKPFKNSDLIASVEIAIRKFKNTKQQQTIVVKDGEYNVALPLEKINYIESNKNYLLF
                     HTNEKTYKTRSTIKKTMKELPDAVFVQIHRAFIINKNKVEKYTRKSVIINNNEIPVSF
                     SLKEFQYL"
     gene            complement(22369..23469)
                     /gene="ychF"
                     /locus_tag="PLS16_02735"
     CDS             complement(22369..23469)
                     /gene="ychF"
                     /locus_tag="PLS16_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNJ60024.1"
                     /translation="MGLQCGIVGLPNVGKSTLFNAVSNNAKAQASNYRFCTIEPNVGL
                     VDVPDERLNAISALVNPNRIVPTTIEFVDIAGLVKGASKGEGLGNKFLANIREVDAII
                     HVIRCFEDDNILREEGNINPVGDKEIIDTELQFKDLESIEKKISKIEKQARVGNDAKA
                     KQEFDILKMCQQHLMEGKSIRTLNLSKEDKVATADLMLLTEKPVLYVANVDEASILTG
                     NKYSDALIAMAKAEHAEVIVLNNSVEAQLSDMSDDDRALFQSEYGLTEPGLNRLIRSA
                     YSLLNLITYFTAGVQEVRAWTIHTGWKAPQAASVIHTDFERGFIKAEVMAYDDFIHFG
                     SEAACKENGKLRIEGKEYIVKDGDLMHFRFNV"
     gene            23684..24271
                     /locus_tag="PLS16_02740"
     CDS             23684..24271
                     /locus_tag="PLS16_02740"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNJ60025.1"
                     /translation="MQKLFTLLLVVTTTLMTFGQSTLNANTTKSSVLWTGRKITKSHN
                     GEVKIKSGNLIVKNDVLHGGKFVIDMNSMTCEDIKDAEYNGKLLGHLKSDDFFSVEKH
                     PTATINITKVFKIKGKENTYSALANLTIKGITNEIKIPVTLKKNSNGKYIAESKFNID
                     RSKWDVRFGSDSFFDNLGDKAIKNDIEFAVVVETL"
     gene            complement(24440..25084)
                     /gene="trmB"
                     /locus_tag="PLS16_02745"
     CDS             complement(24440..25084)
                     /gene="trmB"
                     /locus_tag="PLS16_02745"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNJ60026.1"
                     /translation="MGKNKLFKFADLVNKINVFDKDISLKGKWQQDFFKNGNSIVLEL
                     ACGKGDYSIGMATQFPDKNFIGVDIKGNRIWSAATITEKLELKNIAFIRDQIDKIDTY
                     FLPEEVSEIWITFADPFPRKSDHKRRLTSAKFLPIYKKILKPNGIIHLKTDSDILYEY
                     TKEVLAEIPSEIIKDYPDVYAMNKNEELFNIQTYYEKMHLKNEKTIKYICFRLL"
     gene            25223..25801
                     /gene="folE"
                     /locus_tag="PLS16_02750"
     CDS             25223..25801
                     /gene="folE"
                     /locus_tag="PLS16_02750"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNJ60027.1"
                     /translation="MSDFNYNDDTTKKLAALYSKVLDGLGEDTAREGLLKTPERVAKA
                     MQYVTSGYSINPTAIIQSAIFHEDHNEMVIVKDIELYSLCEHHMLPFFGKAHIAYIPN
                     GKITGLSKLARVVDAFARRLQVQERLTVQIRDCINDALDPHGVAVVIEAKHMCMMMRG
                     VEKQNSITTTSAFTGEFTHDVTRSEFLRLIGR"
     gene            complement(25787..26854)
                     /locus_tag="PLS16_02755"
     CDS             complement(25787..26854)
                     /locus_tag="PLS16_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HNJ60028.1"
                     /translation="MKTIDTKITKLLCDLIETPSLSRNEEDAAIVMRKFLKKEKIPFE
                     IRANNTWAKNKHFNFLKPTILLNSHIDTVKPVSGWTKEPYKAIFESDKLYGLGVNDAG
                     AALVCLLGTFLHFYEQEDLNYNFIFAATAEEEISGKNGIELISDIVNSCSLAIVGEPT
                     EMELAVAEKGLMVVDAEVKGKSGHAARNEGVNAIYATLKDLDWIENYQFKKINPYLGP
                     VKCTVTMINAGTQHNVIPDSCKYVIDIRTTPEYSHEQVLEVLGDNLKATIVARSTRLN
                     PSSINSEHDIVKAANKLGINTFGSATLSDQALIAIPSVKIGPGKSERSHTADEFIYVR
                     EIEEGLATYVKLLSQLVLSAN"
     gene            complement(26854..27648)
                     /gene="argB"
                     /locus_tag="PLS16_02760"
     CDS             complement(26854..27648)
                     /gene="argB"
                     /locus_tag="PLS16_02760"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNJ60029.1"
                     /translation="MDKKELLVIKIGGNVIDDNAFLSKFLEDLAAIPQPKILVHGGGK
                     LATQLAAKLGIETQMIDGRRITDSETIKIVTMVYAGLVNKKIVAKLQANKCNAIGLSG
                     ADAQLIQAHKRKHPTIDFGFVGDIDSVNSDFLQKLLEDAYTPIIAPISMDNAGVLLNT
                     NADTIASTVALAMSSVFKVKLFYCFEKQGLLSDIKDENSIIPTIKIEEIQQLIDNKVI
                     VDGMIPKVQNIKFAIQQGVDSVCLCHASNLLDISRESSNFGTTFLN"
     gene            complement(27641..28414)
                     /locus_tag="PLS16_02765"
     CDS             complement(27641..28414)
                     /locus_tag="PLS16_02765"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNJ60030.1"
                     /translation="MELNYKKYGEQGPYLIILHGLLGSLDNWQSIAQILSEHFQVYSI
                     DQRNHGRSPHSSEINYQILAEDLEQFILKHQLENVVLIGHSMGGKVAMHYALNFPDKL
                     SKLIVVDIAPKTYEGGHENILELMTNFDLKSVNSRQEIETHFVERLRSKALVQFVTKN
                     IGRKSDNSFYWKCNLEAIVNNYEQLMKFPASKKQFLGSTYFIKGLLSDYIQESDSFFI
                     KKYFPSAKVFEIKNASHWVHSENTADFVNTLKYILQENG"
     gene            complement(28421..>29222)
                     /locus_tag="PLS16_02770"
     CDS             complement(28421..>29222)
                     /locus_tag="PLS16_02770"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNJ60031.1"
                     /translation="EFAEHRLYNPGDSIKEIDWKVFARTDKLYTKKYEEETNLRCQIV
                     IDNSSSMYFPKDKKDGAINKMEFSVIASAALMYLLKKQRDAVGLSIFNDDLKLHRLPK
                     TNAQHHQMLLSDLYHLIHQEAKQQLTSAAKSLHLIADAIHKRSLVFIFSDMMDSTNED
                     ELFLALQHLKHNKHEVVLFHTLDKKHEVDFNFENRPYQFIDMETGESIKLQPNQLKDT
                     YLQQMQEKIIRLKSKCIQYKIDLIEVDIAEGYYPILMQYLVKRKKMKI"
BASE COUNT        10365 a         4605 c         4171 g        10081 t
ORIGIN      
        1 tagtaatttc tcactactta atactttcta ctcaatactc aattaattgt ctgaatctgg
       61 atttatttta ataaagcaat atctaaaacg tcttgcattt tatcgacata atggaatttt
      121 aaacctttta taaattcagc ctgaatttca tttacatctc tttcattttg attacacaaa
      181 attatttcct taattccagc acgtttggcg gccaatattt tttctttaat accaccaact
      241 ggcaatactt tacctcgaag tgtaatttct ccacttaatg ccaaatatgg tttgattttt
      301 cgttggctgt ataatgatgc cagagccgta aacaaagcaa ttcctgcaga aggtccatct
      361 tttggaatag caccttctgg aacatgaata tgcaaattat tttcttcaaa aacttttgta
      421 tctattttca aattagaggc aatagattga aggtacgtta atgctgttgt tgcggattct
      481 ttcataactg tacctaaatt tcctgtcatt gttaaactgc ctttgccttt tgaaagtgta
      541 gattctaaaa ataaaatatc gccacccatt ggtgtccacg ctaatccaat agcaactcca
      601 agtggtaaat ttttagtgta taaatcgtgg ttatactttt cttttccaag tactaatttt
      661 actttttcag tatctatatt cggtacatac tcttcttcta aagaaataga tttggcaata
      721 tttcgcatga tagatgctag tactctatct aactcacgaa cgccactttc ataggtatat
      781 ttagtgatga tatattgtaa aacgctagga cttattttaa tttgatttgc ttttaatccg
      841 tgtgcttctc tttgtttttg aaccaaatgt tttttagcaa tttctacttt ttcttcaact
      901 gaataaccac tcaactgtat tatttccatt ctatcacgca aaggtgcagg aatctctgca
      961 atattattgg ctgttgcaat gaataacact ttagacaaat caaattcttg ttctaaataa
     1021 ttatcataga atgtagaatt ttgctctggg tccaacactt ctaacaacgc tgaacttgga
     1081 tctcctctat ggtctttgcc tattttatct atttcatcta agataaaaac aggatttgca
     1141 ctttgtactt ttttaagcga ttgtattact cttccgggca atgctccaat ataagttttt
     1201 cgatgccctc gaatttcact ttcatcatgc aatccaccca aagccattct aatatatttt
     1261 ctatctaaag cacgagcaat actttttccc aaagacgttt tcccaacacc tggaggtcca
     1321 acaaaacaca agatgggcga ttttaaatca ccttttaatt tcaaaaccgc taagtattct
     1381 aaaattctat ctttaatttt atctaaacca aaatgatctt catctaaaac tttcttggca
     1441 tattttaagt caaaattatc cttagtatat tcattccaag gtaattctac catccaatct
     1501 aaataattta aaatcacaga atattctgcc gctgctggat ttatacgttg cagttttcct
     1561 atttcttttt caaaatgctt ggcaactgat tcactccaaa ctttttcttt agccttttct
     1621 tttaagcgat taatttcttc ttgatacata tcgccgccca attcttcttg gatagctttg
     1681 atttgttggt ttaagaaata ttctctttgt tgtttatcaa tatccgtttt tgtttttgaa
     1741 tggatattgg ctttaatttc ttgcacttgc atttcgtatt gcaaatgacg aatcacttgc
     1801 tgaatacgtt gttttaaatc attggcttct aaaataactt gtttatcttt tgcatcaata
     1861 tttaagttgg aagtaataaa gtgcgttaaa aattttactt tgtgtatatt attgatgaca
     1921 atttttgctt cattcggaat ttgtggtgaa atatcaataa ttttttcaga caattctttc
     1981 atcgttgaga tgaatgcttt ttcttcattt tcatcagtta aaggaacatc ttcgacaaac
     2041 ttaatcgaaa cttccataaa aggttcttca gcaatcaaat caacaacctc acattttttt
     2101 acaccttgca aaatcacggt tgtgccatta tctggcatat taattaactt aataatcctt
     2161 gctaaagtgc caattggata caaatcttta aaatcaggct cgtcattatt ggcatctttt
     2221 tgtgtcaata cggtaataaa ttttttatct ttataagctt tttttactgc ttttatagat
     2281 ttatctctac ctacagaaat agggataacg acatcaggaa ataaaacatt atttctaagt
     2341 ggcaaaacgg gtactgtttc aggtaaatct tctgttttta tactctcatc ttcatcaata
     2401 gataaaatag gaatcatttc aacttgatca tcactaaaca tatcagacat ttggttattt
     2461 tcgaacattc tctaattttt aaatactttg acagattgtc ataaaacaat aaacaaactg
     2521 caatatatat catttgcaat aaatatgcct aattaatttt atctgtttgt taatgaagtt
     2581 ttgtcattca ttttcataca aaattaaagt tgtacttctt ttttataaaa aatactataa
     2641 attattagaa atgttttttc ggcattattt tttgtacaga cttttcttaa tttccgctgt
     2701 agttatacag atattgtaac ctttttgttc taatcctctt ttaagttcgt tgtcgagtgt
     2761 ccaaatttta tagtgcttgt atcgatttag tgcaacaaaa aagacatcat caaaatctat
     2821 atctattaca atttcatacg cttcaagata atattttttt ggaatgtctt tggtttcaat
     2881 tatggtaatt aatcgcttat acgtttttat ttccgcttta atctgttttc tgtcaagtgg
     2941 cgacaactct tctattttag acaaatgttt gtccacttcc tcgaacaaat aacttggagc
     3001 gaataactgt agcttacttt ctgctttaag aatgtgtgca attgttccat ttggcgttat
     3061 caatgcactg aaaataatat tactgtcagt aataacaatc atacaccaat taaatcttta
     3121 aacattcgtt tggtcatatt tcggtttact tttttggaaa gttccaaaat ggctttgtta
     3181 atttcctttt tcgatttaac agtaactttt ttttctgttt tcttttttgt ttgtgtcata
     3241 ttattcataa tctttatttt tatacagaaa aaacctaata cttccctaca aaaatacaaa
     3301 aactatttca gtttatagaa ttcagaatat taggattttc aattttttgc aaaaaaatgt
     3361 aagcgagaga aaaatatacc aattgtcctc tactttaagc ggagaaaata gaaaaaagga
     3421 aaaatgaggc tgaccgaaaa gtttagggca gcctccttac agaagataag gcaattttac
     3481 acaaaataat agcattaaat actattattt atttttttac aatacttttg gttgttttgt
     3541 tgtacagaca agttggtaac atcaataaaa aaggtaatgc tttattataa tctaactcgg
     3601 aatattctaa acctaataaa cgaatgtttt tatagtatgt ttgatgttgt tcaaaataca
     3661 atttctcact ttgcataaac catgcctttt tgcctaattt cttagccaaa aaccatttct
     3721 caatcaatct cgcacttctt ccgttgccat cattccaagg atgaatttta acaaatacta
     3781 agtgtataaa cgaagcataa aaaagcactt cttcaaagct catttttatt ttaagtaaaa
     3841 ttgaaatatc attataaagt ttttgcatat cgatttctac ttcgtaagga gaagctgcaa
     3901 catattcaat tcgaccgtca ggtgtggata catacatatt atgtatgcga aattttccct
     3961 gccaattttt gttcacaata tgcttgctca ataatttgtg tgcttctgaa atattatttt
     4021 tattgagttc attggtatta gcaaaagtgt aagcattata caaatcatca atttttttgg
     4081 tataattggg caaaaattca attccaaatt ttttatattt aatatagctg tctaattcaa
     4141 tgtcttcgcc ttcaatttta ctactaaaaa cagatgacac cgaagtataa aaactgaaat
     4201 tatccgtaga aatttcagca tcttttaagg tatcaaaagt cgtttgcaaa ccttttggaa
     4261 cttgctttaa ataagaagca agcaaatcag ttgagattat ttgcagttcg tctgttttga
     4321 taatttataa aattttaaac aaagatataa atatttctat gagttgttag aaacattgtt
     4381 gagtgcatta ggctaaaata aatatcaata tgtttactat attttatacc acttagtgaa
     4441 attttgtcaa aaaaacaaag tgctactttt acttttttag tttttaataa taaaccatta
     4501 tatttatgcc aaagtattat cagatatgaa agcacttgta ttacgagaaa acggacaatt
     4561 acaggtagaa acggtagaaa aacctcaact tggagcaaat caagctttag taaagattag
     4621 ctttgcagca ttaaatcata gagatgagtg gattaggcaa ggtcaatatg ctaaaataca
     4681 aattccaagt attttaggtt cagatggtag tggtattgta gaaagtgtat caaatgaaag
     4741 tgatagcaat tgggttggaa aggaagttat tataaatccc aatcaaggtt ggggagacaa
     4801 tccaaaagct caagccaaaa cctataatat tttgggaatg ccatcacaag gaactctggc
     4861 tgagtatgtt gtagtggata tagatagact aagacaaatt ccacatcact taaatttaga
     4921 gcaaagtgca gcattacctt taggcggtgt aactgcattt aatgttactt tcaataaagc
     4981 taaagtaaag ccacatcaaa atattttgat ttcaggcgtt ggtggaggtg ttgctcagtt
     5041 tgcattttta ttttctgttg ctgctggtgc aaatgtgtat gcaacatcaa gcaaagcaga
     5101 tgtgattgaa aagtgcaaaa cactaggagc aaaaggcggt tgcagttata aagatgaagt
     5161 tgctttgaaa tctatggcaa aagaaatagg aggttttgat gctattatag attctgcctg
     5221 tggtgatgga atgaacttat tactttcctt attaaaacct acaggtagtt ttgcttttta
     5281 tggagcgaca agaggattgc caaaagattt gaatatgaga aatatatttt ggaatcattt
     5341 acaaatttta ggttcaacaa tgggtagcga ccaagatttt gtaaatatgg taggctttgt
     5401 ggagcaatat aaaatacaac ctattataga tacaagtttc tctttagata atgcagtagg
     5461 tgcatttgat aggatgaaaa atggagaaca gtttggcaaa attattgtaa aaatataaac
     5521 ataaaaatta gaaatcgatg aacattatta ttccaatggc gggtagagga acacgtttac
     5581 gtccacatac attagttacg cccaaacctt tagttccagt tgctggaaaa cctatcgtag
     5641 aatggttggc agaagatatt atagcacttt gtccagaaaa agtagataaa attggatttg
     5701 ttattggaga ttttggagca gaggtagagc aaaatttatt gaaagttgca gaacgattag
     5761 gagctgaagg tgttattttt catcaagaac aagctttagg aacggcacat gctattttgt
     5821 gtgctaaaga attacttgat ggaaaagttg ttgttgcttt tgccgatact ttattcaaat
     5881 ccacacaaca actcaatgca gaacatgatg gtgttatttt tgtacagcaa gttgctgacc
     5941 cacgtgccta tggtgttgtg aaaatggatg caaactctcg aattacagat tttgtagaaa
     6001 aaccacaaga gtttgtttct gaccttgcga ttataggaat ttattatttc aaagatggtg
     6061 ttaatctaag agctgaattg caatatttaa tagacaataa tattacagaa aaaggagaat
     6121 ttcaactgac caatgctatg gaaaatatga aaaataaaaa caaagcattt tatcctggca
     6181 aagtagagga atggttggat tgtggtaata aagacgcaac ggtttatacc aatcaacgga
     6241 tattagaaat taaaaaagca gaaattacag cacctattgg attaaataat gttaattcat
     6301 taattataca gccatgttat attggtaaaa atgttacttt agagcataca attattggtc
     6361 ctcatgtaag cattggtgat gatagtaaaa tttccaacgc tattataaaa aatagtatta
     6421 ttcaaaacaa atcaaatatc caaaataaga taatcgaaaa ctcaatgata ggtagctcaa
     6481 ctgcactttc tggaaatata gaaaatctaa gtattggaga tttttctact tcaaattagt
     6541 ctatgaataa attggtatta tatctatgtt tttttgtatt tgcattcgca aataatccat
     6601 tagcattttc aaaagaaaaa aaggcgaaga acaaaaaagg tgcgagtgaa gttgttatac
     6661 ccaagacaaa aaaagaaata ttagctattc aatccatatt tattgaaggt attcaagcag
     6721 atttattagg caataagaaa gatgcaattg ccaagtataa tgaagtaatt agacttgatg
     6781 ccaataatga tgctgcttat tacgaattag ctaaattata ttattcaatc ggcgatttac
     6841 aacaaactgt ttttaatagt cagaaagcca taaaaatcaa tccaaacaac gaatattatt
     6901 atatttattt agcagaagct ttaggcgagg caggaatgta tgcggaagct tctaaaactt
     6961 atgaatcttt gataaagcta aaacccaaag aatacgatta ttattatgat tgggcgtata
     7021 tgctatctca agccaaaaag aacgatgagg caataaatgt attgaactta ctagaaacta
     7081 aagtaggtgt aaacgaagat ttaattatgc taaaacaacc gctttggata caacaaaata
     7141 aagtagaaga tgctgttaaa gatattgata aactgattgc attatttcca agagaagaaa
     7201 tgtattattt gaataaggct gaaatatacg ctacaaataa tttgccacta aaagcaatta
     7261 gcacttatga aaatttatta aaaactcatc ctgaaagtgc cgatgcatta ttgggtttag
     7321 cagaattata cagaaaagaa aataatacta ctaaacaaca agaatatcta aatcaattgt
     7381 ttaataatcc aaaaattgat attgattcaa aaataatggc cattattcct atggtagaaa
     7441 aaatgggaag tgattctact tatgatgaat ctttattaaa aatcgtcagc cttataaata
     7501 aaaattaccc aaataatatg aaagcaatag ctgccaaagc tgatgttttg tataatatgg
     7561 ataaaaaaga agaagctcta aatgaatata taaaattagt tcatcaacat gcagattctt
     7621 tacccggaac aatatggata catacctata ttcttgcatc agaattacaa aaaatggata
     7681 cgctgattga tgttactgaa agagccataa aagttgctcc tgatgatgct ttcgcttact
     7741 tctacaatgc gattgcacat caacagaaaa aacaaaatca agaagcagca atatcaataa
     7801 aaaaaggatt acaaaatatt caaaaaagtg gtctttctgt ttataatagt caattaaaac
     7861 tacaatctct aatagtatta ggcgatattt cctatgaact aaaagattat acttcaatgg
     7921 acagtgctta taatgctgcc ttggaaattg acccaaataa ttccaataca cttaataatt
     7981 atgcctatta tttaagcgaa cgagatgtta atttagttat ggcagaacga atgagtaaga
     8041 aagccaactt attggaagat gacaattctg cttttatgga tacttatgct tggataatgt
     8101 ataagatgaa gaattataaa gaagctttaa tttggataga agatgcgttg aagttacctg
     8161 gtgcaaatca gcgttcggaa ttaatagaac attatggcga tatacttata gaaaatggtc
     8221 gtaaagatga tgctattatt caatggaata aggcaattga attgggtgga gacaaagaag
     8281 ttattaaaaa taaaatcaat aaatataagt gagaaagctg agtatatttt tgtttgctgt
     8341 aattattatt gtagcctgta gtcctacaaa aaaggctaag aaaagcaaaa aaaagaaaga
     8401 cgacacgata acaacggttg ttgataccat taagacacct gaaattccaa aaattccatc
     8461 tgtaaaagac ccagagatac aaaacatctt aaatcaaatt gctgcaaatg aattaaaatt
     8521 tactactttt tattctaaag taaaaacaaa ggcaactatt gatgataaaa cacaatcttt
     8581 taccacacaa ttacgatggc acaaatacaa taaaatttgg atgtctatgt caattattgg
     8641 aattgaaggt gcaagggttt taatcaacaa ggatagtatt aaaataattg accgattaaa
     8701 tagtcgtaat atcttaaaac ctataagtta tattcaatca aaggcttatg taaatttaac
     8761 gtacaatgac attgaaaagc tatttttggc acaacccata ttaatcaata aagataaatt
     8821 agaattggta aaaggcgata aagagaatct attaaaatct tatgacgacc gatttgcaac
     8881 catcatcaag ctagacaaac aaaatagaat aactaatatt ttcatcacag ataaattaaa
     8941 gaaccaaact ttaaattctg attatacaga ttatattctt ttaggtggaa aatattttcc
     9001 aaaaacaaga aatataaaaa ttgtaaacgg tacaaaaaac tttgaaatgt ctatggattt
     9061 tggtgattta gatatatcca aagatttaac tttcccattt caaatcaatc ctaaatataa
     9121 aaatgagtag aattctaatt atactaagtt tccttttgat taatatattt agtattaatg
     9181 ctcaaaccaa taaagagcaa ttacaaaaaa aaagagagca attactaaag gatattaaat
     9241 acacccaaga tttattaaaa actaaaaaat cagataagca agcatctatc aatgatttga
     9301 atgctatcaa ccaacaaatt aatttacagc aaaaactgat acaaaattca caatctcagg
     9361 ttcatttatt tgacaatcaa attatccaat caaaagaggt aataaaaact aaaaccgagg
     9421 aactcaatca gctaaaaaaa gaatacgaac aagctattat caatacttat aaatacaata
     9481 agtttactga taaagttttg tttatcctaa atgccaaatc attttctgaa tctttacgta
     9541 gagtaaatta cttgagaagg tatgccatac atcgagagaa acaagctgag gagattcttc
     9601 aaaaatctaa agaaattcaa aaagatatcg ttgttatcaa ccaaaagaaa gcagagaaac
     9661 aaacggtact taatcaacaa atgagtgaaa aacaagagtt aggaaaaacg caacaagaaa
     9721 aaaataaaat tgttgctaca ttaaaaagtg aggaggcgaa attacaatcc acgctttcta
     9781 aaaaacaatt agaagccaaa aaactagaca atcaaattgc tgcaatcatt aaaaaagaaa
     9841 ttgaagcagc caaaaaagct gccttattag ccgcacaaaa agctgctact gaagcggcaa
     9901 ctaaaaaagc agcagaactt gccgcaaaga aagctgctga aaaggagaaa acaagtactt
     9961 ctaccaaaac aactactacg acaactaaaa caacaacaag tactacaaca acaccagtaa
    10021 aaccaaccta tacaccagaa tatgaaaaat tgacaagtag tttttatggc aataagggaa
    10081 aattaccttg gccagttgaa aaaggattta ttactaaata ttttggcaaa tacaatcacc
    10141 cagatttgcc aggtgtattg attgagaata atggcattga tataaagaca gatccaagtt
    10201 ctggtgttcg ttgtatattt gaaggtactg ttgttggtat aattgacaat ccagtttttc
    10261 aaaatgcggt aattattagt catggtgaat attttacagt ctattcaaaa ttaggcaatg
    10321 taaatgtaag caagggacaa aaagtaaata caaaacaact catcggtaca gtatattctg
    10381 acatagataa tgtaacagaa gtacattttg aggtttggaa aggttcagag aagttaaatc
    10441 ctacgacttg gatttactcg aaataattga ctctatactt tattaaccat tgttaaacac
    10501 tattgtgttc tctattctct atttttaaaa caataaaaaa taaaagatga aatcattcaa
    10561 taaaaaagtg gtggtaataa caggagctgg ctctggaatt ggaagagctt tagcacaaaa
    10621 ttttgcaaaa aaaggtgctt acttagcaat atctgattta aacaaagaag gactcgcaga
    10681 aaccgttcaa ttatgtaata atacaaaagt ttattctgaa atttttaatg ttgcaaaaaa
    10741 agcagaattt caaaagtttg caaaaaatgt attagatgaa tttggtagag ttgatgttgt
    10801 tatcaataat gctggtgttg ctttaggtaa aacaaaatta aatgatacaa cttatgaaga
    10861 atttgaatgg ttgatgggta ttaatttttg gggagttgtt tatggtacaa aagaattttt
    10921 accttcttta ttaaaacaac cagaagctgc agttgtaaat atttctagct tatttggatt
    10981 agcaggcatt ggcgaacaag tgccttattg ttcttcaaaa tttgcagtaa gaggcttaac
    11041 ggaatcttta agaatggaat tattagatac caatgtatca gttcatacag tacatccagg
    11101 cggtattaaa actaatattg caaacaacgc aagaatttct gaaagtgaag atcaagaaca
    11161 agctaaaaaa gtattgaaaa attttaataa agcattaatt catacacctg aaaaagcggc
    11221 tcaagttatc attcgaggta tagagaaaaa acaagaaaaa atcatgattg gttttgaaac
    11281 ttatgtaact gatgtggtta ctaaagtatt accgaccagc tattctaaaa tttttgattt
    11341 actaggtaaa caagcattac agtagtgttt ttattataaa tagtaataaa taagtccttg
    11401 gtttttcaag gacttattta tttaaaatat aaaacaactt acttagtttt tagataacaa
    11461 tatttacttt taccaaaaat aaagtatgaa tgttcacatt attcaacatg aggtatatga
    11521 agcacctggt gcatttttaa gttggattga ggaaaagaat tttactagtt cattttcaag
    11581 agtatttata gatgacccat tacctcaatc tgccgataat attgatatac taattgtaat
    11641 gggcggacct caaagccctg atacaagtat agagcaatgt cctcacttta atgcaaaagc
    11701 tgaaatagca ttaatacaaa aatgcattca aaataaaaaa atagttattg gagtttgttt
    11761 gggcgctcaa ctcattggag aggctttagg tgcaaaatat gaacatagcc ctgaaaaaga
    11821 aataggcgtt tggcctattc aactcactgt agatggatta aaagataata atattaaaca
    11881 ctttggaaca aatttgccag tcggtcattg gcacaatgac atgcctggat taactaaaga
    11941 ttgtaagatt ttagcacaaa gtattggttg ccctaggcaa ataatttgtt attcagattt
    12001 tgtttatggt tttcaatgcc acatggaatt tactccagaa gttgttgatt tacttataaa
    12061 tgaagaagaa gatttcttac agaataatac taaacatgta tttgtacaaa aacctgatgt
    12121 aattagacat caagatttta gtaaaatgaa tcaaaaatta aaagaatttt tagataatat
    12181 ttcatacaaa tatcaacaag ttagataata gactctatct gcaaaagtgt ctaaacttta
    12241 caaaccaaaa acaccattta taatttacaa aatgaaaggg tgttaatgtg tataaaagta
    12301 attgtcaatt aatcaacaga aggctgaatg ctttcctctg ctttaacttc acctaaaatt
    12361 tcttcttcag taattccttc attaaccact tggacttttt cagtatgtgt tgtttcttct
    12421 tcttcaaatg gtcgcttccc aaaaatcaat tctaaatcat ctttgaaaat tacctcacgt
    12481 tccaataaaa tcttagccaa ctgatctact tttgttctat tttctgctaa aatttgtttt
    12541 gctctaatat attgtgtttc tatagtttca gaaatttctt tatctatcaa ctcagcagtt
    12601 ttttcagagt aaggcttttg gaaagaatat tccgattgtc cacttgaatc ataatatgaa
    12661 atatttccaa ttttatcact caatccaaat acttttacca ttgcttgtgc ttgtttagtt
    12721 actttttcta aatcactcaa agcacctgta gagatattac caaaaacaac ttcttcagca
    12781 gcacgaccac ccaaagcagc acacatttca tctagaattt gttcttttgt ggtgatactt
    12841 ctttcttctg gcaaatacca agccgcaccc aatgactgac ctctcggtac tatcgtaact
    12901 ttaatcaaag gatttgcatg ttcggtcatc caactcactg aagcatgtcc cgcttcatga
    12961 taggcaattc ttcgtttctc agcaacctta ataattttat ttttcttttc tagtccacca
    13021 actactctat caatcgcatc taagaaatct tgtttttgca ctgccttctt atccttacgt
    13081 gcagcaacta atgcggcttc gttacaaata ttagcaatat cagcacctga aaatcctgga
    13141 gtttgctttg ctaaaaatgg aacttctaca tcactatcta aaactaaagg ttttaaatgt
    13201 actttaaaaa ttgcctctct ttcttttaac tcaggtaaat ccacataaat ttgtctatca
    13261 aatcttcccg gtcttaacaa tgcagcatcc aatacgtctg gtcgattggt ggcagccatc
    13321 aagataatat ttttatcacc tgagaaacca tccatttcaa ccaaaagttg attcaaagta
    13381 ttctctcttt cgtcattact ttgcatcata ttttttcctc ttgcacgacc aacagcatct
    13441 atttcatcaa taaaaataat acaaggtgct ttttctctag cttgtttaaa taagtctcgt
    13501 actcttgatg caccaacacc cacaaacatc tctacgaaat cagaacctga caatgaaaag
    13561 aatggcactt tagcctcacc agcaactgcc tttgctaata aagttttacc agttcctgga
    13621 ggtccaacta acaaaacacc cttcggaatt ttagctccca atgatgtgta tttttgtgga
    13681 tttttcaaaa aatctacaac ttcatgtact tcttcttttg caccatctaa tcctgcaaca
    13741 tcttcaaaag taatactcac ttttgagtct ttatcattat caattagact agcttttgat
    13801 tttccaatat tgaaaatttg cccgcccatt ccaggcatac cgccagatgc ttttcgcatc
    13861 aagaacatcc atacgccaat gaataagatt atcggtaaaa accaaccaat aatatcaatc
    13921 aagcctctcc tattttcaga tttcaacgga actaattgtt cagttggcaa ttctgcttga
    13981 gctttatcaa atttattttt aaaagattca acggtaacga taggcacaat aaaatgtggt
    14041 cctttatttt ctgaacctaa ttttgtttct ctgatgtctt tataaatatc ttttgatttg
    14101 atagaatctg gtttgatata aacttctgca attttgtcgt ttacaataat tacttgttct
    14161 acatcatgac ttggcaacac ttttctaaaa aattgttcct cagaaatatc attttgtttt
    14221 atattcaatc cataaaactg aaatgccaaa ataccggcca ttatcaaccc gaatatccaa
    14281 tatccgctcg gtccgcctcc gctatttttc ttcttttttt cattctccgg taataaatcc
    14341 atttctttat cctttatttt aaattttata ttctttaatt acagcatcat gccacaattc
    14401 ttctaaacga taaaatgctc ttctttcctt ttgaaaaata tgtaccacaa catctccaaa
    14461 atcaatcaaa caccattctc caaaagaacg accttccaca tgaaatgctt taataccgta
    14521 cttttttgcc atttcatatt ctacataatc agtaatcgca cgtgtttgag tggtactttc
    14581 acaatcgcag attataaaat aatctgtaat tgcttcagga atttttctca aatccataca
    14641 aacaatattt ccacctttct tatcatctat aagattaata ataacctcag taaggtctaa
    14701 tttgtttaat ttttctgttg tttttttctt ttttattgtt tccaagtgtt ttttttctta
    14761 ttttccataa aattactaaa ttttgactac aatcattaac gatacccaat atcttggtaa
    14821 gaatttaata ttattgcaat ctattccttc cacaaattct tatgctaaag aattacttac
    14881 aaaatctaag cctaaaaatg gaccggtaat tttggcagaa gagcaaactt caggacgtgg
    14941 acaatctgaa aatatttggc aaagtaatgc taaagataat ttgacttgta gtgtaatttt
    15001 agaaacacat ttaatacctg cgagtaagca atttttatta aatatggcta tttcattggc
    15061 tgtattgcat actacaagag catttactga agaagattca actatttcta taaaatggcc
    15121 aaatgatatt ttagtacaac aaaaaaaaat ttgtggcatt ttgatagaga atactatttc
    15181 tgggcaatgc ttgcaacata gcattattgg aatcggacta aatgtcaatc agttggattt
    15241 tggaatgcat aagcaagcaa caagtatcca gcaaattaca caaaagcaaa atgatgtttt
    15301 cttggtatta aaaaaacttt tggtagagat ggaacgtcaa ttagctttta ttcaaaatga
    15361 acaatattta aaagaacaat atttaaaata ctttttccaa ttaaatcaga ctgcaaaata
    15421 tatttataaa gaaaaagaag tcttaggaat aatcaaaaat gtcaatgaaa ttggtcaatt
    15481 gatgatagaa attgatggaa aaatacatat tttaaacatg aaagaaatta agtttttgta
    15541 ttagaaaaaa atatttagtt ttcaaacatg caacagacta taaaatttat actataatct
    15601 tttgttctac taagttatta taagcattta agcaagcatt tttttctgct tcttttttat
    15661 tttttccttt gccctgtgcc ataaaaattg tatcaatata aatatcaact acaaattgtg
    15721 tgtcgttttc agggttggct acgtgcttta attccaattg ttttttttct gcttgaaccc
    15781 attcatataa gaaactctta aaatctttta tttcattttc aaattgctcc aaatccacgt
    15841 tttctaacaa taatttacaa attattggtt ttgtaccgag catagtataa tctaaaaaat
    15901 aagcaccaac taatgcttct aatgtgttgc ccaatatatc tggcgaggtt tggtaaaaat
    15961 ttggagctaa tttatgtttg atatagcgtt gtaaattcag atttaatcca attttattaa
    16021 ggtgtgtacg actaactaat tgtgaacgca atttgctcag ttcgccttca tttttggaat
    16081 caaaatgatt gtacaaaaac tcagcaatca caaaattaat aacagaatct cctaaaaatt
    16141 ctaatcgttc atttttattt tgaatatgct ttgaataaga agcgtgtgtt aatgctttga
    16201 tataattgga ttcggagtgt ttgggtaaat aacctattga ttggtataaa tctttatatt
    16261 ttaatacttc tgctaatact gaattttcag tttttatctt tctaaaaata tttttaataa
    16321 aagaaaacat ctttagttgg taaacttttt aaaaattaat gaggtattat gtccaccaaa
    16381 tccaaaatta ttattcagta catattttat atttctcttt tgagtctgac ctaatgtaag
    16441 attcaaatcc attccaattt gctcatcaat ttcctttgtg tttatagttg gtggcacttg
    16501 ttgatattga attgctaaaa tagaaataat actttcggtt acaccagcag cacccaacaa
    16561 atgtcctgtc atagattttg gagcactaat atttatagaa gataaatctt ttccaaaaac
    16621 agtttttaag gcattaattt ctgcaatatc acctattggc gtagatgtag catgcgtatt
    16681 tataaaatca atcggcttat ttaatatttg agcatttgct aaagcttgtt gcatgttttt
    16741 gataatcatc tctcctgctg gacttggagc tgtaatatga tgagcatcag cattcatagc
    16801 atatccacta atttcggcta aaatattagc tcctcttttt tgtgcatgtt ccaaagattc
    16861 aatcactaat gcaccagcac cttcgcccat tacaaaacca tctctatttt tatcaaatgg
    16921 gcgacatgca ttttcaaaat tatcattctt ttcagataga gctctcaagg cattaaaact
    16981 tccaattcca gcttcgtgga tgggtgcttc tgaaccagca actatgattg catctgccat
    17041 acccaattga atattttgta ctgctgaaat tattgcatga gcagatgacg cacaggctga
    17101 tacagtagaa aaattgatgc ctttaaatcc ataatgaata gaaatataac ctgccactgt
    17161 atcaattagc atctttgtaa caaaataagg gcttaaatat cttggattat tagcttgttc
    17221 ataataagcc ttcatttctt gttgaaatgt ttggacacca ccaacgccag aagccgaaat
    17281 cacgccaacc ttctctgtgt tcaattcagt ataatctaat aaattagcat ttttgatggc
    17341 ttcacgagtg gctactaaag catattgagt aaacaaatca tattttttga tttccttttt
    17401 ctcaaaaaaa tcttcaggct gataattctt aacctcacaa gcaaattgag ttctaaactt
    17461 tgaagcatca aatttagtaa ttgagcctat cccagatttt cctaaaaata aattattcgt
    17521 aaaagattgg atattatttc caataggagt aattacccca tagccagtaa tcactacagg
    17581 ttttagaaac ttcatttatc ttttactatt ttatttatcg tgtatgactt tatacattgg
    17641 actctaagaa tgcgattgct tcgccaacag ttttaatttt atcagcttgt tcatcgggaa
    17701 taactaagtt gaattctttt tcaaactcca taattaattc cacggtttca agcgaatctg
    17761 cttttaaatc catttcaaaa tttgcttcag gtgtaatttc tgtttcttct acgcccaatt
    17821 tctccacaat gattttcttt acgcgttccg ctatttcaga cataatctct atgtttaatt
    17881 tcctgacaaa ggtaagtttt attttataat ttagatgacc tacttaaaaa aatattaaca
    17941 aaaggaaatt tgccttaatt attctttgag taaattttaa ctcattctgt ggttaaattt
    18001 tcttatttta tctgcttttc tttctgtctt gatacaaaaa gaaacaaaaa aactagaaca
    18061 atctaagaca tttttgtcaa cgaacaaaac ttcagctcga aaaatctgct gttataaacc
    18121 atcttattga tttaatttct tctttactct aaaattaatg caaaattttc aacaaaacaa
    18181 aatttgtttg gcttgcacgt aggtttttgg tcgcctagat ttttcttcgc ctcaatgctt
    18241 aagattgttc aacataaaca ttgattcttt tcttctaaat aattttctat aaaaaataaa
    18301 cgatttgttt ctaaattaat cgataattca tttatttaaa ccgcacaacg ggtttgattt
    18361 tgcatattta tatccccaaa atcgccattc tatcccagaa aattttaaac aaaaaaaatg
    18421 tagttcatat ttgccatata ttaaaatgca ggaagccgaa aatgcaattt aagagtaggc
    18481 aagattttaa atttaataca tgaacttaac aaaaatttta ggagtcgtag caattacatt
    18541 tatatgtatt acgagtgctt ttgcccagaa aaaacaatta aacgtaggtg aagaatttcc
    18601 tgttgctgga caaggaagca atcaaactat tgtgtctcaa aatggacaat atgctttaag
    18661 taaagattat agtgccggaa atgatggtag attttatacg catctagttt ctaatccgaa
    18721 ccaaaaaatt gtagaatttg gagctcctca ctactcaaat actagactta aaattaataa
    18781 tggttggctt aatatggtag gtataaaccc taaaactaat agcgaaccta atagtgtttt
    18841 atttatttat tctaatggaa atggccaaaa tacaaatggt ggtggtgtaa aattaattgt
    18901 agaaaacgat ggttctcttt cattatatag tgctgctgga gcaaaagttt ggtctaagac
    18961 atcgccaaat gttaaacaag aaattggacg taatacactt aaagctggag aaactttaaa
    19021 agctggagaa cgtttaatct cttcaaatgg tgcttacatt ttaagaatgc aagaagatga
    19081 tggtcattta tgtgtgtaca aatttgaaaa cggaaaacaa ggaaattttg tttggggtac
    19141 tggagagcat ggttttaaaa atgcttcatt gatcatgcaa gcagatggaa atcttgttgt
    19201 atatgatggt agtaatgtag caaaatggtc ttctaaaaca catccttatt ttgatcctaa
    19261 atttaatcag gcagaaaaca agccagtaaa attagtattg gaaaatgatg gtagcttaaa
    19321 attatacata gcatctggaa gtgtaatttg gacaaacaaa taaagtttat tagttataaa
    19381 taatttagaa tgtgaatttc agtagtagca tttttataaa taaaatccct aacctttcgc
    19441 aattaattaa ttgaaaaggt tggggatttt taatttgtaa tgaaaaacaa cacatcaata
    19501 tacataataa tttcttttaa tatcaattat tcttagattt gattcattaa taatatcact
    19561 ttgttttatc agatttttat tttgctattt ttattttcta accatattgt gatggctaaa
    19621 aatttctttg cagaaaaaga attaaaagaa atagacagct tgataatata taatcacttt
    19681 gagattgcag aaaaaagaac ggaaatttta tcgaaaaaac ttcaaaataa taaaaaatat
    19741 aaaaagcatt ttttagaaac taagtttaga caagctttaa ttttagacaa aaaaaacgaa
    19801 tctcccgcaa aacaacttca gcttttacta aaaataaaag atgaggcaga aacagaaaat
    19861 ctttttccgt tgatgtatcg tatttatctg ttgatagcat tgtctcacga aaaatctaat
    19921 aattttgatt taaccaaaaa atatttagac aaagcattcg ctacttataa aaagcatcgt
    19981 ttagatagta ttttcagtac ttattgcatt agaaatagct cgtatcttag atttattaat
    20041 gaatctgatt ctagttttta ttatgctaaa aaagcaaatg aatttgctaa aaaatatgac
    20101 aatgaagaag atttgaaaga ttcttatatt ttattaggaa attttgaatc ccgaaaagaa
    20161 aactatctcg aatctttgaa atattattat cttttattgg attatataaa aagtctgaat
    20221 aaatatttaa taggtgtagg ctacaacaac atttctagag tttacctaaa aatgcaagac
    20281 ataccaaaag cattactgta caatgattcg gcatatattt attacgaaga acaaacgctg
    20341 atgtacagaa attattttcc tgaaacatga tataaaatct atgaggaaat ggggaaaact
    20401 gattctgctt tttattattt caaacaatat catactgatt ttgaagcctt gaaaaaagaa
    20461 gaagaattag taaaaacaaa agaacttgaa gaacaatatc aaaatgacaa aaaagaagcg
    20521 accataaaaa gtaaacagca acaaatactt ttaattattg gtttgcttgt tgtaattgct
    20581 tgtagtttgg tgatgcttta ttttaaaaac agaaaaatta ataaacaaaa taaaattata
    20641 ggagaacaat taattgaact taccaaaact atagaacaaa aagaagtgct tttgtctgaa
    20701 cttcaacata gagtaaaaaa caatctacaa catgttatta gtattttaga aattcagaaa
    20761 gaatcggttg attttaataa tattgatgaa ctaattagag gcaatcaaaa tcgtattcac
    20821 tcaatggcat tgttgcataa taaactaaac attgctgaca atgttgctga tgtgaatttg
    20881 aaacaataca ttactgaact tgcagaatta gtaagagatt cttatgacaa tatcaaacag
    20941 gaaattcatc ttgatattaa atgtaaaata gaaaatattt ctataaaaaa agcattgcct
    21001 ttaggtttaa taatcgtaga acttgtgagt aacagcataa aacatgcatt caaaaaacag
    21061 cataacagaa ttatcaatat tgaaattaca aaagaagaaa ctatgaaatt atactatgca
    21121 gacaatggca ttggtttcga ttttaacaaa ctgaataaaa aaggattggg acaagaaatt
    21181 attaaaggat tgattgatca aatagacgga aaaattgaga ctaaaaacaa caatggtttc
    21241 gaattgataa tttattttgt ataaagttta acaaaaaaaa atgaacgaaa aaaaaatttt
    21301 acttgtagaa gatgattttc taaatagacg actttctaaa aaaattttta tagaaaataa
    21361 ttatcatgtt ttagaagcta aaaacactaa agaagcatta gaaatcctga aaacagaaac
    21421 agtaaattgt gccattttag atattaatct tggtaaaaaa gaagcagacg gaatttcgct
    21481 tggaaaacaa atttatgaaa acttttctat tccatttatt tatcttactg cttatgaaac
    21541 tacagaaatt atagaaaaag cagttgcgac ttcaccctat tcttacctta caaaaccttt
    21601 taaaaacagc gatttaattg catctgtgga aatcgctatc cgaaaattta aaaacacaaa
    21661 acagcaacaa acaattgttg taaaagatgg agaatacaat gtagcacttc cgctcgaaaa
    21721 aattaattat atagaatcta acaaaaatta tttacttttt catactaacg aaaaaacgta
    21781 taaaactcgt tccacaataa aaaaaacaat gaaagaactt cctgatgctg tttttgtgca
    21841 aatacataga gcattcatta taaacaaaaa taaagttgaa aaatatacaa gaaagagtgt
    21901 tataattaat aataatgaaa ttcctgtatc tttttcctta aaagagtttc aatatctgta
    21961 atgtaaactt ctttatgtcc tggttttcta taaaaattac agaatttact gtttgttacg
    22021 taagcattgg ttgtatttac atttctatca atttgccacg taaccatatt gtctggcttt
    22081 tgtattaaac gaagcttata tccaatgtgc atctactcgg ttgttggtgc attttcaaac
    22141 aaaaaaatac cttcttcgat gcttaaccat ccgaagttta atgcttttta atatagttgt
    22201 ttgagttgag acaatttcta gtcttgagtt gatagttatt tgatgtccgt tgtttaaaat
    22261 tactaaattt aattattata cattaaaatt aaatcagact gctttgtcgt ttcagtttta
    22321 ctattgaaac ttctcgcagt gacgaaaata actttttata attctaaatt aaacgttaaa
    22381 tctaaaatgc attaaatcac catctttaac aatatattct ttaccttcta ttcttaattt
    22441 accattttct ttacaagctg cttcactgcc aaaatgaata aaatcatcat aagccataac
    22501 ttctgcttta ataaatcctc tctcaaaatc tgtatggatt acacttgccg cttgtggcgc
    22561 tttccaacct gtatgtatgg tccaagcacg cacttcttgt acaccagccg taaaataggt
    22621 tatcaaattt aataaagaat aagcagatcg aatcaatcta ttcaaaccag gttctgttag
    22681 tccatattct gattgaaata aagctctgtc atcatcactc atgtctgata attgagcttc
    22741 aacagaatta tttaaaacga taacttctgc atgttctgct tttgccattg caattaaagc
    22801 atcagaatat ttattacctg tcaaaattga agcttcatca acattggcta catataaaac
    22861 tggtttttct gttaaaagca ttaaatcagc agtcgctacc ttatcttctt tggaaagatt
    22921 taaagttctt atactttttc cttccattaa atgttgttgg cacattttta gaatatcaaa
    22981 ctcttgtttt gcttttgcat cgtttccaac tcttgcttgt ttttcaattt tggaaatttt
    23041 cttttcgata ctttctaaat ctttaaattg tagttctgta tcaataattt ccttatctcc
    23101 tactggatta atatttcctt cttctcttaa tatattatca tcttcaaaac aacgaattac
    23161 atgaataata gcatcaactt cacgaatatt tgctagaaat ttattaccca aaccttcgcc
    23221 tttacttgca ccttttacca atcctgcaat atctacaaat tcaattgttg taggtacaat
    23281 tctatttgga tttactagag ctgatattgc atttaatctt tcatctggca cgtccactaa
    23341 gcctacattt ggttcaatgg tacagaaacg ataattactt gcttgagctt tagcattatt
    23401 actaactgca ttaaataaag tagattttcc gacatttggt aaaccaacta taccacattg
    23461 taatcccata tttataattt aaaatttcag accgcaaagg tacaaaaaga ataagtaatt
    23521 ctctgtattc taaaaaaata taaaaacact aagcgaaatt gctcaaattt cacatacagt
    23581 ttcattgata tatatcgata ttttttgtat tatttaatat taacattatt ttagagaata
    23641 gcattcaaaa agaatataac tttgcattag aaacaaatta agtatgcaga aattatttac
    23701 attattattg gttgttacaa caacgttgat gacatttgga cagagtacat taaatgcaaa
    23761 tactacaaaa agtagcgttt tatggacagg cagaaagatt acaaaaagtc ataatggcga
    23821 agtaaaaatt aagtcaggta acttaatcgt aaaaaatgat gttttacatg gtggtaagtt
    23881 tgttattgat atgaattcaa tgacttgtga agacattaaa gatgcagaat ataatggaaa
    23941 attacttggt catttaaaaa gtgatgattt tttttcagta gaaaagcatc caacggcaac
    24001 aattaacata acaaaagtgt ttaagataaa aggaaaggaa aatacttatt cagcattagc
    24061 taatttaacg attaaaggta ttactaatga aattaagatt cctgtaactt taaaaaagaa
    24121 tagtaatgga aaatatattg ctgagtctaa gtttaatatt gatagatcaa aatgggacgt
    24181 acgtttcggt tctgattctt tctttgataa tttaggagac aaagcaatta aaaatgatat
    24241 agaatttgca gttgttgtag aaacattgta aatttacagt atcattgttc tttgcaagag
    24301 ggaacaatgg gtttttcata gttttatggt ttttgtggta agtcctgctt gaaaaagcgg
    24361 gacttaccta tttaaggcat ttcttaaacg attaattcat aagacaataa aaaaattgaa
    24421 ccaacaacaa ggatttattt tacaacaatc taaaacaaat atattttata gttttttcat
    24481 tcttcaaatg catcttttca taataggttt gaatattgaa caactcttca tttttattca
    24541 ttgcataaac atctggataa tctttaataa tttcagaagg aatttctgct aaaacttctt
    24601 tagtgtattc atataaaata tcactatccg tttttaaatg aataatacca ttaggtttta
    24661 atatcttttt ataaatgggt agaaattttg cagatgtcaa tcttcgttta tggtcagatt
    24721 ttcttggaaa tggatctgca aaagtaatcc aaatttcact tacttcttca ggcaaaaaat
    24781 acgtatcaat cttatcaatt tgatctcgaa tgaatgcaat atttttaagt tctaattttt
    24841 ctgtaattgt tgcagcactc caaattctat tgcctttaat atcaacacca ataaaatttt
    24901 tatcaggaaa ttgtgttgcc attccgatac tataatctcc tttcccacaa gctaattcta
    24961 aaactatact attaccattt ttaaaaaaat cttgttgcca ctttcctttt aatgaaatat
    25021 ctttatcaaa aacattgatt ttattaacta aatcagcaaa tttaaagagt ttattctttc
    25081 ccactttttt tgtcgcaaaa atacatgttt tattttgttt ttagtaaata ctaagaactc
    25141 aaataggtca aattcctttt gaaattaggc tttcttatta ttttatccta gatttgcagt
    25201 acgctaaaac aaaagaaacc gaatgagtga ttttaattac aacgacgata ccactaaaaa
    25261 actagctgct ttatacagta aagttcttga tggacttggt gaagacacag cacgtgaagg
    25321 tttattaaaa acgccagaac gtgttgcaaa agcaatgcaa tatgttactt ctggttattc
    25381 aataaatcct actgcaatca tacaatctgc tatatttcat gaagaccaca acgaaatggt
    25441 aattgtaaaa gatattgagt tatattcatt gtgtgaacac cacatgttac cattttttgg
    25501 aaaagctcat attgcttata ttccaaatgg aaaaatcact ggattaagta aattagcacg
    25561 tgtagttgat gcttttgcaa gaagattaca agtacaagaa cgtctaactg ttcaaataag
    25621 agattgtatt aatgatgcat tagatccaca cggagtggca gttgtcatag aagcaaagca
    25681 catgtgtatg atgatgcgtg gtgtagaaaa acaaaattct attacaacaa catcggcatt
    25741 cactggggaa tttacacacg atgttacacg ttctgaattt ctaagattaa ttggcagata
    25801 agaccaattg cgataataat tttacataag tagccaagcc ctcttcaatt tctcgaacat
    25861 agataaattc atctgctgta tgagaacgtt ccgatttacc tggtccaatt tttactgatg
    25921 gaatagcaat taatgcttgg tctgataatg tcgcagagcc aaaagtattt atacctagtt
    25981 tattagctgc ttttacaata tcatgttcac tattaatgct tgacggattt aaacgtgtag
    26041 aacgagccac aatcgtcgct ttaagattat ctcccaatac ctctaacact tgttcgtggc
    26101 tgtattctgg agtcgttcta atatcaataa catatttaca actatccgga atgacattgt
    26161 gttgtgtccc agcatttatc attgtaactg tacattttac aggacccaaa tatggattta
    26221 tttttttaaa ttgataattt tctatccaat ctaaatcttt caaggttgca taaatggcat
    26281 ttacaccttc atttcttgca gcatgtccag attttccttt tacttctgca tcaaccacca
    26341 tcaagccttt ttcagctaca gccaattcca tttctgtagg ttctccaaca atagctaaac
    26401 tacaagaatt gacaatatca ctaattaatt caataccatt tttccctgaa atttcttctt
    26461 ctgcagttgc agcaaaaata aaattatagt ttaaatcttc ttgttcatag aaatgtaaaa
    26521 aagtacctaa taagcaaact aaagcagctc cagcatcatt tacacctaaa ccataaagtt
    26581 tatctgactc aaaaattgct ttataaggtt cttttgtcca gccactaaca ggttttacgg
    26641 tatcaatatg agaatttaat agaattgtag gttttagaaa attaaaatgt ttattttttg
    26701 cccaagtatt atttgctcta atttcaaaag gaatcttttc ttttttaaga aatttacgca
    26761 ttacaattgc tgcatcttcc tcatttctac tcaaagaagg tgtttcaatt aaatcgcaaa
    26821 gtaacttagt aattttagta tcaatagttt tcattagttc aaaaaggttg ttccaaaatt
    26881 actgctttca cgactaatat ccaataaatt ggaagcatga cacaaacata cgctatcaac
    26941 gccttgttgt atggcaaatt taatattctg gacttttgga atcataccat caacaattac
    27001 cttattatca atcaattgct gtatttcttc tatttttata gtcggaataa ttgaattttc
    27061 gtctttaata tcactcaaaa gtccttgctt ttcaaaacaa taaaataact ttaccttaaa
    27121 aactgaagac attgctaaag ctactgtcga tgcaattgta tcagcatttg tatttagtag
    27181 caccccagca ttatccatag aaattggagc aataattggt gtataagcat cttctaataa
    27241 tttttgcaag aaatcactat ttacactatc tatgtctcct acaaatccaa aatctatagt
    27301 tgggtgtttg cgtttatgtg cctgaattaa ttgagcatca gcacccgaca aacctattgc
    27361 attgcatttg ttcgcttgta gtttggcaac aattttttta ttcactaatc cagcataaac
    27421 catcgttact attttgattg tttcagaatc tgtaattcgt cttccatcaa tcatttgagt
    27481 ttcaattcct aattttgctg ctaactgtgt ggctaatttt cctccaccat gcaccaatat
    27541 tttaggttgt ggaattgctg ctaaatcttc taaaaattta cttaaaaatg cgttatcatc
    27601 aatcacattt cctcctatct taattaccaa aagttccttt ttatccattt tcttgtaata
    27661 tatattttaa agtattaaca aaatcagccg tattttcaga atgtacccaa tgacttgcat
    27721 ttttaatttc aaaaactttt gcactaggaa agtatttttt tataaaaaaa ctatcgctct
    27781 cttgaatata gtctgacaat aatcctttta taaaatatgt gcttcctaaa aactgttttt
    27841 ttgatgctgg aaatttcatt aattgttcat aattattgac aatagcttct aaattacatt
    27901 tccaataaaa gctattatcc gattttctgc ctatattttt agttacaaat tgtactaaag
    27961 ctttacttct taaacgctca acaaaatgag tttctatttc ttgccgtgag tttacagatt
    28021 tcaaatcaaa attagtcatc aattctaata tattttcgtg tccaccttca taagttttgg
    28081 gtgcaatatc tacgacaatt aatttagata acttatctgg aaaatttaaa gcataatgca
    28141 tcgcaacttt cccgcccatt gaatgtccaa tcaacacaac attttccaat tgatgtttga
    28201 gtataaattg ttctaaatcc tccgctaata tttgataatt aatttctgat gaatgcggcg
    28261 aacgtccatg atttctttgg tctattgaat aaacttgaaa atgctctgat aaaatttggg
    28321 ctatagattg ccaattatcc aatgagccta aaagtccatg taaaataatt agataagggc
    28381 cttgctctcc atatttctta taattcaact ccactgcaat ctaaattttc atctttttac
    28441 gttttactaa atattgcatc aaaattggat aataaccttc agcaatatcc acttctatta
    28501 aatcaatttt atattgaatg cattttgatt tcagtcggat aattttctct tgcatctgtt
    28561 gcaaataagt atctttcagt tgattgggtt gtaactttat agattctcct gtttccatat
    28621 ctataaattg ataaggccta ttttcaaaat taaaatcaac ttcatgtttc ttatctaaag
    28681 tatgaaataa aacgacttcg tgtttattat gcttcaaatg ttgcaatgct aaaaacagtt
    28741 catcttcatt cgtactatcc atcatatctg aaaaaataaa aactaaagaa cgtttatgaa
    28801 tggcatctgc aatcaaatgt aaagatttcg cagcagaagt taattgttgt tttgcttctt
    28861 gatgaattaa atgatacaaa tcagataaga gcatttggtg gtgctgtgca tttgttttag
    28921 gtaagcgatg aagcttcaaa tcatcattaa atatcgataa gccaacagca tctctctgtt
    28981 ttttcaacaa atacatcaac gcagctgaag caataacaga aaactccatt ttattaattg
    29041 ctccatcttt cttgtctttt ggaaaataca tcgacgaaga attatctatc acaatttgac
    29101 aacgcaaatt agtttcttct tcgtatttct tagtatataa tttatcagta cgcgcaaaaa
    29161 ctttccaatc tatttctttt atactatctc caggattata caaacgatgc tctgcaaact
    29221 ct
//
LOCUS       DAOQCW010000017        28508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_618_length_28508_cov_6.114891, whole genome shotgun sequence.
ACCESSION   DAOQCW010000017 DAOQCW010000000
VERSION     DAOQCW010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 28508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28508
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_618_length_28508_cov_6.114891"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PLS16_02775"
     CDS             <1..608
                     /locus_tag="PLS16_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNJ60032.1"
                     /translation="CLVAVGRKPYTENLGLENIGIQPNQRGQIEVNDHLQTNVPNIYA
                     IGDVIRGAMLAHKAEEEGVLVAETIAGQKPHINYNLIPGVVYTWPEVAAVGQTEAQLK
                     ANGVKYKSGSFPMRALGRSRASMDIDGFVKVLADENTDEILGVHIIGARAADMIAEAV
                     VAMEYRASAEDISRMSHAHPTYTEAIKEACLAATENRAMHI"
     gene            741..2297
                     /locus_tag="PLS16_02780"
     CDS             741..2297
                     /locus_tag="PLS16_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNJ60033.1"
                     /translation="MLILRNLLKFRIVIALILFGVSLQLLAQKDYVRVDGTIFRDADG
                     RMLMLRGMNYVNKDKANKFLNLTKDEAFVNLKKWGYNTVRMGVNWSGLEPHPGQYDTT
                     YLKDLDTRLEYARQHGIYVILDMHQDLYSIKFGNGAPLWATLDNDLPHYTGGVWSDAY
                     YISPAVQTSFDNFWKNTPAADGIGVQDHYINTWAMLAERYKNDKNIIGFDVMNEPFIG
                     TAVEQVLGVMLMEMTDALNAINPEKQYSVEEVGGMWIDEQGKNFILQNLTDKDVFVKV
                     LEKMEPIYSHFEKSALMPFYTNFAKAVRKVNTEHILFWEPSVSSNNGIPSSISVVSEA
                     GTQQAYMSHLYDIVLDTELSGEADGNRVEYMFHQLATSREKLNLPTIVGEWGAFYSGS
                     KELMGAAKLMTDGIDSLLFGDFYWSYYKEMGNQILFQKVLLRPYMQAATGKILKQTIQ
                     GNTFEISWQEAKKSKSKNLIFLPYTKNLNIQGKVKYNKIDLENSGSCILEIAPIKKSK
                     KRQLTISWED"
     gene            2386..3642
                     /locus_tag="PLS16_02785"
     CDS             2386..3642
                     /locus_tag="PLS16_02785"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNJ60034.1"
                     /translation="MRTYIPYIFFAVILIVDSYLYFNLSKNILVQSKHKKKLLLGYLF
                     LCSITYITLIIANIIGYKDWAGSWFKNLMFGFSISFFLARFLILPILLLLDLIRLFFW
                     VADKKSDSVIQQNNGISRLTFFYKSALVLGGSLFGGFIYGVIKGAYNIRTNYIKIKIK
                     SLPDEFKQLKIVQISDLHVGNFLNTHHLENMVQKINDEKPDFVFFTGDLVNFKSDELI
                     PFLPTLKKLNANRRIYSILGNHDYGEYYDWENATAKEKNLQDLVAMQEQELGWRLLRD
                     EHELLDYRGYQMAIIGVEYWGHSMHFGKKGNIQKAYSGAEEADLHLLLSHDPSHWDKE
                     IVTNEKYQSIDVTFSGHTHGFQFGVEIPKLNFQWSPSKFVYPHWAGLYQIKEQQLYVN
                     RGMGYVGYPGRLGIPPEITVFNFTSV"
     gene            3693..4448
                     /locus_tag="PLS16_02790"
     CDS             3693..4448
                     /locus_tag="PLS16_02790"
                     /inference="COORDINATES: protein motif:HMM:NF014590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNJ60035.1"
                     /translation="MFDNFTIQQFIYIWIGIALLIFPIQLFVSAPYGRHSKTTWGPMV
                     PNRLGWILMEGWALIAILFFYIKFYNPNPYSLLFIILYALHYIHRGFIYPMRTRTKGK
                     LNPILIVLFAMTFNSINAGLIGYYLSKVSIYPKDYYLHWNFIIGIILFISGFIINFKS
                     DDMLIHLRKPGETGYKIPKGFLFKYISCPNHFGEMIEWLGFMLMVWNWAGIAFFVWTV
                     SNLLPRALHHHKWYKQNFPEYPKERKAIFPFLL"
     gene            complement(4463..5527)
                     /locus_tag="PLS16_02795"
     CDS             complement(4463..5527)
                     /locus_tag="PLS16_02795"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNJ60036.1"
                     /translation="MTTKLSITKNYQVLKKNNYNVYIEILSEKISFVNLYSCPNYKSS
                     QQNQNTALDLPYISQFFVAKVAINDLEDVLNNSGKVHRITLIFPNVGFCRNSFYQLVK
                     IISDSGINFELFFNFKEHINYLEYSLNELRNIVPVSKIKILEANNTDYKLSNIKCEGI
                     NFLIKRFVDITASLSAMIILSPVFIITALLIKLNGARSVFYKPTRVGFYGKSFKMFKF
                     KSMYEDDFSGIRSTVENDPRITPIGRFIRRHNIDELPQLYNVLKGDMSLVGPRPHRVF
                     LSESLHSEIPNYSLRYLVKPGITGWAQANGWRGPTETEMQKRERTLHDIFYVGNWNIF
                     FDIKIIIMTILGTKSKKNAF"
     gene            complement(5862..6305)
                     /locus_tag="PLS16_02800"
     CDS             complement(5862..6305)
                     /locus_tag="PLS16_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60037.1"
                     /translation="MKNISKIILVLFTVFTLSCSNDELGNVDLGEYYGPYIHFKSGAK
                     EVIPAGDTLDIPVSIYVPQWENFTVQYELRGSYSQTGTFTVESGNREDLLQLIIPVNP
                     SITSDVVDTLFLVSATNGVKIGRQNVSSNNIIKKPIKIIAPDSGE"
     gene            complement(6317..7729)
                     /locus_tag="PLS16_02805"
     CDS             complement(6317..7729)
                     /locus_tag="PLS16_02805"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HNJ60038.1"
                     /translation="MKKKTLLICSIMMVLLVTSCRKGYFDINTDPDAATTVDPVYLLN
                     NSAGAYTAERMAEVYGTRLYTQMWLGGDYNYWEDEIFIVSPYTTNNFWSTTYTDALTN
                     AQQALEMSKKKFVNPKNAVAQINVWKAFIYYNTTMLWEDVPFSEVGQIETNSAPHYDS
                     QKDVFNGILGLLDEAIADLDVSNTEKLPDPWFRGDITKWIKLARSLKLKVLMTMVDAD
                     PSKATDIGNLIDAGGMMTSAADNWKFQYLNESGKKNPIWYIVEKYYGSTQEAWLASNV
                     VLDILQSDSDPRLEKFYTIGDDASDYIGIDPFEPISDRVPEISYVSTDIINGGYPDYM
                     HTFAEQELLIAEAIQRGFASGDANAKYLSGVDASLKHYGVAPGEASAFISTLADLTSL
                     SSTDALEAIHKQQYLNLFDRTIEPWNQWRRTEYPALTIPLDASLSDIIRRLQYSPTEL
                     SSNPNAKPNKPLDTKMWFDL"
     gene            complement(7747..10890)
                     /locus_tag="PLS16_02810"
     CDS             complement(7747..10890)
                     /locus_tag="PLS16_02810"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNJ60039.1"
                     /translation="MKRALALWILGLIVSVQVFAQNRSISGTVTDATTGEALIGVSVS
                     AKGSVVGTITDFDGKYTLEVPKEITGLVFSYVGYTTMEKPITSLVINAALATDQAVID
                     EVVVTAVAVKKEKKSIGYSATTVSGETLNKVKETNLLNALSAKTPGVVISSQSGSIGS
                     GSKITIRGVSGLSGGSPLLVIDGTPVSNLGNTGTRNQILGGGVDQGNKYSEINPDDIE
                     TMTVLKGGAATALYGSQASNGAIIITTKKGAVSKTKVPTVEVGVNVFTDHALVLPNYQ
                     NEYAQGTNGVYVLKNLNGWGPKISAVQNQKFLDFKGDSVTLKANPNNVKDFFENGMTY
                     ITNFALSNADETGDYRFAYSNVTQRGILPNSKLGRHTFTINAGKKINKYIESRVSASL
                     VANNTFGRTRQGSNNESIIPSIVLGLPRTVDINDVKNHVFDELGNLVGLDGAASNQVN
                     NPYWITQNNPVSDKGFRFYGTGEVIIRPTSWYTFTSRIGTDYISENRTAITRKGTLRM
                     LPGAYSTTDIVRNQLNSDLINTFYKKWKNIDVSYLVGLNIRNVSSRLVDVAAQDMIVD
                     QLYTYANAKTITTTESKVKTRLLGVYHNLNLGLWNSFFIDASFRTDINSTLPKENRVY
                     NYYGVSGSFVPLSLIKNYNDKWVSYIKLRASYAQVGSSYSAYLTNFTYTPVSSVFGQF
                     GTNLTFPYGDLVGYETTSAYPSQTLKPSRQNDWEVGGEFNFFRNKLRIDYSYYQKVTK
                     DNIISLPISASTGFNAKIINAGSVSNKGHELLVTATVYESKNRDYSIGLNMNFTKNKQ
                     KLEELYPGLKDYVLTSGFSGFQIKAEEGQTFGLYGTAYLRDSATGKYIINAGTGLRQQ
                     APSPKRFGDIYPKWNMGLGADIYIKGLTISVLFDFRKGGVMYSGTTAGLRTSGLAAET
                     AINRDGTIIDDGVLLNPDGTTRPNDVPVRSMEDYWKNNYQTSITEANVFDASYIKLRQ
                     LALSYSLPAKLFENSKTVKGFTIGVSGRNLALLKSNVPHVDPESNVFGTSLIGEGYEF
                     FNSPSTRSIGFNLNVKF"
     gene            11265..12752
                     /gene="guaB"
                     /locus_tag="PLS16_02815"
     CDS             11265..12752
                     /gene="guaB"
                     /locus_tag="PLS16_02815"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNJ60040.1"
                     /translation="MENIAHLSHNQKFAGEGLTFDDVLLIPAYSQVLPRETNISSKVT
                     KNIDINIPVLSAAMDTVTEAELAIALAREGGIGVLHKNMSIEQQALQVRKVKRTESGL
                     ILDPITLHPDNKVADAFSIMKDHKIGGIPVVDNQNILVGIITNRDLRFCKDPNMFIKD
                     LMTKENIITAPEKTNLKQAEEILHQYRIEKLPIVGKDNKLVGLITYRDIINVKNYPNS
                     CKDEGGRLRVAAAVGVTPDMMQRIEALVDGGVDFISIDTAHGHSKGVIDAVKNVKQTF
                     KNLDVVAGNIATGAAAKALAEAGVDAVKVGVGPGSICTTRIVAGVGVPQLSAVYEAAL
                     ALKGTGIPVIADGGIRYTGDMVKAIAAGANTIMAGSLFAGTEESPGDTTIFEGRKFKV
                     YRGMGSLEAMNDGSKDRYFQDVEEDIKKLVPEGIVGRIPYKGKLSEVVYQYIGGLKAG
                     MGYCGAKDIATLQETAQFVRITSAGSVESHPHDVSITNEAPNYSR"
     gene            12887..13453
                     /gene="pth"
                     /locus_tag="PLS16_02820"
     CDS             12887..13453
                     /gene="pth"
                     /locus_tag="PLS16_02820"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNJ60041.1"
                     /translation="MSKFLIVGLGNIGNEYADTRHNIGFNIVDTLADQLKVNFTVDKL
                     AMYANGTYKGKQIHIIKPTTYMNLSGKAIKYWAEFLKIDFENILVILDDLALPFGTLR
                     LKSKGSDAGHNGLRSIQTELQTQNYPRLRFGIGDNFQKGKQVNFVLGKWSVEEQKKLE
                     DRILLACEMTLSFCFAGINNTMNSYNNK"
     gene            13476..14300
                     /locus_tag="PLS16_02825"
     CDS             13476..14300
                     /locus_tag="PLS16_02825"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HNJ60042.1"
                     /translation="MLELFQIFFNHKKYILGFTFLAILLSIIITIPAIIPPKFQSKTI
                     FYVANPLSTDRANLFNQKDGGIVSQFGDRNDADRFIAISTSSNVLDAIIKDFNLIKHY
                     KINKENHNLNLYFAKKKLLKNLNIKRNDNDAIEITCLDTDPEIAAKIIQNLIQQTNSN
                     YSKINKDNKQKVLTELEVLIKSKKEALTQLSGIEKTTALESILELENLKSQYLVSLND
                     NFASIYVIDAAEVAVKKSYPIRWQIVLLTAFFAFATALLFAIVYQLYEQHAKATKV"
     gene            14707..15732
                     /locus_tag="PLS16_02830"
     CDS             14707..15732
                     /locus_tag="PLS16_02830"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNJ60043.1"
                     /translation="MKTSIVKTLYLLVFYLGTALFINQFNKIKKLIWVLWIPTFLTIV
                     YALIIHSSYNFSFESINKALHPIYRNHVNYGVFITMLFPFLIFLNRQSKAQTIPKLFT
                     QISIPITLVAIYFTYTRGAWLGLLSMPIYYIVLKLKWTKSILIIVSITILSFGSYLFY
                     NNNYQKFAPNFNKTIYHDKLSAHLNSTFEMEDMSTVERFYRWLASIKMSKKNLIYGVG
                     PGNFTENYKPYTVASYRTYISENEEKSTVHNYFLLMLAEQGLVGLLIYVAFVFYLFIY
                     IEKLYHKQIKKEHKVAVLLIACSILAFLFNNTLSDFLETNKVGSLFWINIGLLTIFSN
                     PIKENEH"
     gene            15722..16315
                     /locus_tag="PLS16_02835"
     CDS             15722..16315
                     /locus_tag="PLS16_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60044.1"
                     /translation="MSIRLKFQTIETNKNEAHYVSEHDAEIIFNFLKKTKTIQRTLQI
                     GLGDGALAFVVLSFTNKLHIAIDPDQSKVQFEGIKNLKRFELFDLVDFRDDYSSAVLP
                     KLLEGNVLPDLTILDNDQTFDKMINDFVNIDLICKKGSIILIRKSKNPNNYLLHQYIT
                     KNRVDYSIVELELEHFVLYEKIARDIRSINDFQGINC"
     gene            complement(16312..16725)
                     /locus_tag="PLS16_02840"
     CDS             complement(16312..16725)
                     /locus_tag="PLS16_02840"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNJ60045.1"
                     /translation="MKIELERKNNAVYLEAKNDDGIIVKMDGSPEIGGENLGARPMQM
                     VLMALGGCSSMDVLSILKKMRENVKSYKVIVEAERAETEPKVFTKIHVHYILSGIIKK
                     ENAEKAIQLSMDKYCSVSKMLEKSAELTHSFELID"
     gene            complement(16732..17193)
                     /locus_tag="PLS16_02845"
     CDS             complement(16732..17193)
                     /locus_tag="PLS16_02845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HNJ60046.1"
                     /translation="MTKYFLTLLSICFIVACSSKYEFAKYESVNENGWNANDTLTFKV
                     KIEETNTPYNINLGVRYNKGYEFDNIWFKLFGNIIEKGKEGKMQEVILFDKIGKPYGE
                     CTGSVCTQIYPIQKNYIFKKSGIYIVKVVHLMRQEPLLGVKDVGIMVEKVK"
     gene            complement(17224..18333)
                     /locus_tag="PLS16_02850"
     CDS             complement(17224..18333)
                     /locus_tag="PLS16_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNJ60047.1"
                     /translation="MNIGFDAKRLYANPTGLGTYSRTLFNRLVEFYPDENYKLFVHKK
                     YFDTTPFKYAYFTKNTVVSEHYSDKIWRQKAIVEDVSSNNIQIFHGLSNELPSLKKEV
                     KKVVTIHDVIFKKLPKTFSIFDRNIYKYKTQKALENADAVIAVSQQTKEDILELFKVE
                     ESKIHLIYPTWNKEYEHDCSYALKEEFIVKYDLPRDFVLYVGSISKRKNFITLLKAMA
                     LPENQDKNLVVVSNGGDEYQEAQSFIYEKKMENRIFFLNNIMWYELPIIYHISQGLIY
                     PSLYEGFGLPILEALVCGKSVITSNISSMPEVGGDACIFIDPKNVEELSNAINFIYYN
                     LELADEIRYKAAAQVQKFQPEKITEQIYQLYQTLL"
     gene            complement(18367..19401)
                     /gene="thiL"
                     /locus_tag="PLS16_02855"
     CDS             complement(18367..19401)
                     /gene="thiL"
                     /locus_tag="PLS16_02855"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNJ60048.1"
                     /translation="MESRTEISKIGEFGLIDRVTKDIATYNASSIKGIGDDAAVVNSA
                     QHQVLTTDLLIEGIHFDLVYTPLKHLGYKAIMVNLSDVYAMNAFPKQVLVSFGISNRF
                     SVEAIDEIYAGMKLACQRYQVDIVGGDTTSSPQGLVISVTAIGEQSFEKIAYRNGAKE
                     GDLLCVSGDLGAAYMGLKILQREKQVFLNAPEASIELDKFTYIVERQLKPEARKDIIQ
                     FFDTEQLVPTSMIDISDGLSSEILHICQQSNVGCTLEEEHVPIANETYDTALSLNIDP
                     ITCALNGGEDYELLFTINPKDKAIVDKNHDIAIIGEITDALLGIKLHTKGGNYHDIIA
                     MGWDGMKKNV"
     gene            19497..20615
                     /locus_tag="PLS16_02860"
     CDS             19497..20615
                     /locus_tag="PLS16_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNJ60049.1"
                     /translation="MNIGIVANTSFNIYNFRLPLMHFLKAKGHHIIAIAPKDNFTEKI
                     IAEDFPFVEIKTLSRKGTNPIQDLKLILELKKIYQQTNLDIVLQYTIKPNIYGSFAAK
                     LANIKCICTVTGLGYTFLNNSIASKVSKWLYRTAFYFSDIILFQNEDDKATFLNLKLA
                     NERKIKIVPGSGIDIYRFDKSFCTNTITDTNVVFLFIGRLLKDKGIFEFIEAAKKIIQ
                     QYPKTLFLIVGEIDQQNPSSISQQELEKITLIPNIKYLGFLQDTRVAICQATCVVLPS
                     YREGLPRVILEAMAMSKPCITTNVPGCKDAVDSSCAFLAEVENSEDLKLQMEKFIVLD
                     NQKKLEMGTNARRRTETIFSANIIAETYFKLISSLVKK"
     gene            20655..21767
                     /locus_tag="PLS16_02865"
     CDS             20655..21767
                     /locus_tag="PLS16_02865"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNJ60050.1"
                     /translation="MHKTEFIIPAAILSSFLLTYSFIPSIIKIAEIKHLYDNPDERKL
                     HGCSTPNLGGIAIFSGFLISFCLFSVIDSAYQIRPIIAALCLVFLIGAKDDIVPLVPY
                     KKFIGQIFAAAIVVVIGKVRISSMYGLFGISMLNDYVSILLTIITIVFIINALNIIDG
                     IDLLAGGVGFIAASTFAVWFYYYGFINYAVMGAALAGAILAFLGFNYSPAKIFMGDSG
                     SLTIGLLLSLMAIEFIEKNEVVLRSGNSEGIAILSSPSMAIAVLIIPIFDTLRAFALR
                     ILKGKSPFYADRIHIHHRLVDLGLTHIHASFVLFAFNILFIVSVYFLQSLGNMTLLIG
                     EFAIGSLFTFLLFSIKKKKKSNSTIHKKAQNLIETY"
     gene            complement(21771..23336)
                     /locus_tag="PLS16_02870"
     CDS             complement(21771..23336)
                     /locus_tag="PLS16_02870"
                     /inference="COORDINATES: protein motif:HMM:NF017050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HNJ60051.1"
                     /translation="MANLEYDYVIIGSGFGGSVSALRLAEKGYKVLVIEKGKRLGAED
                     FPKTNWNLRKWMWMPKLGMQGLFKLTFFRHVAVLSGVGVGGGSLVYANTLPVPKSEFF
                     NSGHWNGLENWEEELKPFYALAKKMLGATKHPYTSISEKVMKELAERIGKPEAFDKTE
                     VAVYFGKQDVTVPDPYFDGKGPTRTGCNQCGGCMLGCRYNAKNTLDKNYLYLAEQLGV
                     KVIAEKEVFDVIPLSIDGADGYEIHFKDSLSYFPKKDSVKTKSVIFSGGVLGTVDLLL
                     KLKEKSLPNLSDKVGTGIRTNSESLIGVTSFDKDAVFSKGIAIGSIINIDESRHIEPV
                     KYSDGSGFWRIFMAPMVQGGNIFTRFYRMVKDLFVHPITNLKAAFIDDWSKRSQILLY
                     MESIDSTLSFKRNILGGLKSNLDTGPAPTAFNPKAQELANHVAEIINGKAMTLSTETL
                     FGIPTTAHILGGACMGNNAQEGVIDKDNFVFNYKNMMVCDGAMISANPGVNPSLSITA
                     ISERAMSKIPNKE"
     gene            complement(23428..24585)
                     /locus_tag="PLS16_02875"
     CDS             complement(23428..24585)
                     /locus_tag="PLS16_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60052.1"
                     /translation="MNKYFLLFILVFTFLSCKKEKQQECDLSSALNANLTEAQQQELL
                     KEVAKKTTPTTEISAEERAFFGDIRVIANDIPRLYNGVTNDMTLEEKYEQLKHNIRSR
                     ANEVEAKALVHYNGSVAYTTMAKNSFVLMTYDALQRNVPEMMWTNLGIFAANEVRGGV
                     VLASQIVNYLEKNNIKIPLKDTTQNIGQMLTDATVLLLEGQLNVVVDIGALVLLNKYV
                     DRDLDSESAWLTPEAIKGFKFQKQAEQALRCGQKEAYQDLQTLAAIQFGIHEQTYILQ
                     PLWDKPLLGLMGQLNEMVLKMTNGQYGIFGEIFIGANKYTEPQKGYNIKIPKGSYNLV
                     NIVHRVDIAMNGFNTYNRLRKQKDWAYWIDYSQIRLGYGVDVYYGTPMGME"
     gene            complement(24595..25497)
                     /locus_tag="PLS16_02880"
     CDS             complement(24595..25497)
                     /locus_tag="PLS16_02880"
                     /inference="COORDINATES: protein motif:HMM:NF013197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60053.1"
                     /translation="MGLFKKLTENKLFESVNNAQQAIKTTGKLISLISDNKVSASVRE
                     MLQGLKTILTEKPNINSINHFINHFLLQISPENQPIIIKELLEVFHERWKNVDRKTAE
                     VALKTYDFADKTILVHGNDIAITALVENLNVNLKTCNIIQIISLHDKIGKQQASKIAE
                     IGIPVTVIDDSAIANYIQDVDVVLMGNEIAMHETFIGKTGSFNLCLIAKYFDKPVYVL
                     SDTRKILNKKYFPTSVVDMFIGKNKKPIVEIWKDAPSNINVNYVHLEEIPNNLVTKFI
                     FENDAYTPEELEDEVEKTMIVKFI"
     gene            complement(25504..27795)
                     /locus_tag="PLS16_02885"
     CDS             complement(25504..27795)
                     /locus_tag="PLS16_02885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNJ60054.1"
                     /translation="MLKRISTFILLVSIVIFVQAQNLPLGTWRSFMPLSNVVSFTQSN
                     QNIYAATEYGVFSVDVDNHFIETYTKSDGLSEVETQKIAYNKEYSVLIIVYKNANIDL
                     IQNGKITNLPYLKNANIISSKTVRNIATKDSLAYFAVGFGVVVVDLIKKEIKETYNFF
                     DGSNPLACNFIALDNSKIYVATENGIFQTELNNPLILNFSTWQKISDGNTGGNISAIA
                     KFDNKIWVAKDHQIYNYDNTNWALVFNELNWKTIDLSVDNNHLYLVQHKTDNSSQRIG
                     KWENNTFSFLAPNSNIRFPLEVLEDKNGNIWHADLYDGLIKDTPNAQRFIPNGPSAIT
                     NKDMNFIGNTLYVASSNAGSNANPTFNKNGIYIYKEGIWTSYGQYNWQLFDTIYDITI
                     AQPLTAENKIILGGNMPGGGIVEMNVSDNSVIVPKVYRPNSTQKFRITNSTVDAANNV
                     WLTDAYSSFPLICRKTDGNYKFFNNVFLNNTILTDVVEDDYGQIWISKNSSSGGLTVF
                     NHNYTIDDISDDQYVNYSVGENNGNLPSNNILCLEKDHNGEIWLGTSQGIAVIRCAGS
                     GIDRNCPAEQICIGRNDGTDFCDNLLEDQTVTVIKVDAANRKWIGTNNGIYLVSEDGE
                     TQIHYFNKDNSPLLGNEIRSIAIQPNTGDVFIGTNLGICSYRSTATETTSESGNAFVY
                     PNPVQSNYQGIITIKNIPNNCNVKITDAVGNLVYETQATGGQATWDGKLINGGKAASG
                     VYLVLCKGIDKKEKLATKFVFLH"
     gene            27869..28321
                     /gene="dtd"
                     /locus_tag="PLS16_02890"
     CDS             27869..28321
                     /gene="dtd"
                     /locus_tag="PLS16_02890"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNJ60055.1"
                     /translation="MRVVIQRVKNASVSIDAKIVGQIDKGLLVLLGIENNDTDKDVEW
                     IFKKIVQMRIFSDEADKMNLSIQDIGGNILLISQFTLFASTKKGNRPGFTEAARPEKS
                     IPLYEKCIATLNTLIGKKIETGMFGADMQVQLINDGPVTIIMDSKRPE"
     gene            28321..>28508
                     /locus_tag="PLS16_02895"
     CDS             28321..>28508
                     /locus_tag="PLS16_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60056.1"
                     /translation="MEIKIHSNLINVIRQTLELIFNQNKIADKEIPKALKNKQYGSKD
                     RAFLAETMYDIVRWKLKY"
BASE COUNT         9667 a         4567 c         4184 g        10090 t
ORIGIN      
        1 attgcttggt agctgttggc agaaaacctt atacagaaaa tttaggatta gaaaatattg
       61 gaattcaacc caaccaacgt ggacaaattg aagtaaatga tcatttacaa accaatgttc
      121 caaatattta tgctattggt gatgtgattc gtggtgctat gttggcacac aaagctgaag
      181 aagaaggcgt attagttgca gaaactattg ctggtcaaaa accacatatc aattataatt
      241 taataccagg tgttgtttat acttggccag aagttgctgc agttggacaa actgaagctc
      301 aattaaaagc taatggcgta aaatataaat ccggaagttt tccaatgcgt gccttaggaa
      361 gatcgagagc gagtatggat attgatggtt ttgtaaaggt attagctgat gaaaatacag
      421 atgaaattct aggtgtacat attatcggag caagagcggc tgatatgatt gcagaagcag
      481 tcgttgcaat ggaatatcgt gcaagtgctg aagatattag cagaatgagc cacgctcacc
      541 caacctatac agaagcaata aaagaagctt gtttggctgc aactgaaaat agagcaatgc
      601 atatatagta gttgaaaagt tttaaattac taaaattttt taaatccttt taatagactt
      661 aactttgtgg aataggaaga gatatctaat tctcttccta tttttttata cttaaaaaat
      721 atacaaaatt gaaatataat atgttgattc ttagaaattt attaaaattt aggatagtta
      781 ttgcattaat attatttggc gtaagcttac aattattagc acaaaaagat tatgtaagag
      841 ttgatggaac aatatttaga gatgcggatg gtcgtatgtt gatgctacgt ggtatgaact
      901 atgtgaataa agataaagcc aataaatttt tgaatttaac aaaagatgaa gcatttgtca
      961 atttaaaaaa atggggatat aatactgtaa gaatgggcgt aaattggtca ggattagagc
     1021 ctcatcctgg acaatatgat accacttatt tgaaagattt ggatactcgt ttggaatatg
     1081 caagacaaca tggaatttat gtaatcttag atatgcatca ggatttgtat agtattaaat
     1141 ttgggaatgg tgctccttta tgggcaacct tagacaacga cctccctcat tatacaggag
     1201 gcgtttggag tgatgcttat tatattagtc cagctgtaca aacttctttt gataatttct
     1261 ggaagaatac acctgcagca gatggaatcg gcgttcaaga tcattatatc aatacttggg
     1321 cgatgttggc agaacgctat aaaaatgata aaaatattat tggttttgat gtgatgaatg
     1381 aaccttttat tggaactgct gtagaacaag ttttaggagt gatgttaatg gaaatgacgg
     1441 acgcattgaa tgcaattaat cctgaaaaac aatactcagt agaagaagtg ggaggaatgt
     1501 ggatagacga acagggaaag aattttatct tacaaaatct aacggataaa gatgtttttg
     1561 ttaaagtatt agagaaaatg gaaccaattt atagccattt tgagaaaagt gctttgatgc
     1621 cattttatac taattttgct aaagctgttc gtaaagtaaa tactgaacat attttatttt
     1681 gggaaccaag tgtttctagt aataatggaa tcccatcttc tattagtgtg gtttcagaag
     1741 ctggtacaca acaggcttat atgtctcatt tatatgatat tgttttagat acagaattat
     1801 caggtgaagc agatggaaat agagtagaat atatgtttca tcaattagca acatcaagag
     1861 aaaaattaaa cttacctaca atagttggag agtggggtgc tttctattca ggaagcaagg
     1921 agttaatggg tgctgcaaaa ttaatgactg atggaataga ttctttatta tttggagatt
     1981 tttattggag ttattataaa gaaatgggca atcagatttt attccaaaaa gtattacttc
     2041 gtccatatat gcaagcggct acaggaaaaa tattgaaaca gactatacaa ggaaatacct
     2101 ttgaaatcag ttggcaggag gctaaaaaat caaaatctaa aaatttgatt ttcttaccat
     2161 atactaaaaa cttaaatatt caaggaaagg taaagtataa taaaattgac ttagaaaatt
     2221 ctggaagttg tattttagaa atagctccta ttaagaaatc taaaaaacgt cagctcacaa
     2281 tttcttggga agattaactg tttgtttgaa attaaaagct tattttaatt tgatttaaac
     2341 atatttaaga gaaagatttg tttctttcgt cttacttttg ctaaaatgag aacatatata
     2401 ccttatatct tctttgctgt aattttaatt gttgatagct atctttattt caatttaagc
     2461 aaaaatatcc ttgtccaatc caaacataag aaaaagctac ttttaggcta tttattttta
     2521 tgtagtataa cgtacattac attaattatt gccaatatta ttggttacaa agattgggct
     2581 ggaagttggt ttaaaaattt aatgtttggt ttttctatct cctttttttt agcaagattc
     2641 ctcattcttc caatattatt gcttttagat ttaatacgct tatttttttg ggttgcagat
     2701 aaaaaatctg acagcgtaat tcaacaaaac aatggcataa gtcgattaac ttttttctat
     2761 aaatctgctt tagttttagg tggttcatta tttggaggtt ttatctatgg tgttataaaa
     2821 ggtgcttata atattcgcac caattatata aaaatcaaaa taaaaagttt gccagatgaa
     2881 tttaaacaat taaaaattgt tcagatttca gacttacacg taggcaattt cctaaataca
     2941 catcatttag aaaacatggt tcaaaagata aatgacgaaa aacctgattt cgtatttttt
     3001 actggcgatt tagtcaattt taaaagcgat gaattaattc catttttacc tactctaaaa
     3061 aaactaaatg caaatcgacg tatttattcc atattaggta atcacgatta tggagaatat
     3121 tacgattggg aaaatgctac tgctaaagaa aaaaacttac aagatttggt agctatgcaa
     3181 gaacaagaat tgggttggcg attattgaga gatgaacatg aactattgga ttatcgtggt
     3241 tatcaaatgg caatcattgg tgtggaatat tgggggcatt ctatgcattt cggaaagaaa
     3301 ggaaatatac aaaaagctta ttcaggtgcc gaagaagccg atttacattt attacttagc
     3361 catgatccat cacattggga caaggaaatt gtaactaatg aaaaatatca atctatagat
     3421 gttacatttt caggacatac acacggcttt caatttggtg ttgaaatacc taaattaaat
     3481 ttccagtggt ctccatcaaa atttgtatat ccgcattggg ctggactata tcaaattaaa
     3541 gaacagcaat tatatgtaaa cagaggaatg ggttatgttg gctatccagg aagattaggc
     3601 attccaccag aaattactgt atttaacttt acatctgtat aatttaaact gtttcttata
     3661 aatattttta ataaaaacca taattttgag caatgttcga taattttacc attcagcaat
     3721 ttatctatat ttggataggc attgcactcc ttatttttcc gatacaacta tttgtttctg
     3781 caccttatgg cagacattca aaaacaactt ggggaccaat ggttccaaat agattaggtt
     3841 ggatattaat ggaaggttgg gctttgattg ctatcctctt tttttatatc aaattttata
     3901 atccaaatcc atattcatta ctatttatca ttttatatgc cttacattat atacatcgag
     3961 gttttatata tccaatgaga acaagaacaa aaggaaaatt aaatcctatt ttaattgtac
     4021 tctttgctat gacgtttaat tcaatcaatg ctggtttgat tggttactat ctatcaaaag
     4081 taagcatata tcctaaagat tactatctac attggaattt cattataggc atcattcttt
     4141 ttataagtgg ctttattatc aattttaaat ctgatgatat gcttattcac ctaagaaagc
     4201 ctggagaaac aggttacaaa attcctaaag gatttttatt caaatatatt tcttgcccta
     4261 atcattttgg agagatgata gaatggttag gttttatgtt gatggtttgg aattgggcag
     4321 gaattgcctt ttttgtgtgg acagtatcca atttgttacc cagagctcta catcatcaca
     4381 aatggtataa acaaaatttt cctgaatatc ctaaagagcg aaaagcaata tttccatttt
     4441 tgctataata gttctcaata acttaaaaag cattcttttt agactttgtt cctaaaatag
     4501 tcatgataat aattttaatg tcaaagaaga tattccaatt tccaacataa aaaatatcat
     4561 gtaatgttcg ctctcttttc tgcatttcag tttctgtcgg accacgccaa ccattcgctt
     4621 gtgcccaacc agtaattcct ggctttacta aatatcgaag tgaataatta ggaatttcag
     4681 aatgtaagct ttcactcaaa aatactctgt gtggtcttgg ccccactaag ctcatatctc
     4741 cttttaaaac attatacaat tgtggtaatt catcaatatt atgtcttctt ataaaacgac
     4801 caataggagt aatcctagga tcattttcta ccgtagatct tatccctgaa aagtcatcct
     4861 catacatact tttaaactta aacattttaa aagattttcc atagaaacca acacgagtag
     4921 gtttataaaa aacagaccta gctccattaa gctttattag taatgcagta attataaata
     4981 cgggggacaa aataatcatt gcggataaag atgctgttat atcaacaaat ctttttatca
     5041 gaaaattaat accctcacat ttaatattac tgagcttgta atctgtatta ttagcttcta
     5101 atattttaat tttagaaact ggtacaatat tccttaattc atttaaacta tattctaaat
     5161 aattaatatg ctctttaaaa ttaaaaaata gttcaaaatt aataccacta tcgctaatga
     5221 ttttaactag ttgataaaac gaatttctac aaaaaccaac atttggaaaa attaaagtaa
     5281 ttctatgaac ttttcctgaa ttatttagaa catcttctaa atcattgatt gcaactttag
     5341 caacaaaaaa ttggctaata taaggtaaat caagagcagt attttgattt tgttggctgg
     5401 atttataatt tggacaagaa tataagttta caaaacttat tttctcagac aagatttcta
     5461 tatatacatt ataattattt ttcttcaaaa cttggtagtt tttggtgata gataattttg
     5521 ttgtcataag tggggtgttt ttatgtttaa tagacgatta ttcttttaaa aaaagcttca
     5581 taagcaattg taaattttaa tgtaataaat agctgtttca taatgcaaga gtaattaaaa
     5641 tagttttggt taaagttaaa tattaaattt gaaataaata aaaaaataaa gtaccagaat
     5701 tttggtattt ttaaaaaaaa gagagattgc ccaaaaagca aaatgaaagc gatgagaatt
     5761 acttaaacga aatcttaatc catcacttcc aaaataaaaa gaggctgaca aaaatttttt
     5821 ggacagcctc ttgtaataaa gattaagatt aaaaaataaa attattcacc agaatcaggt
     5881 gcaataatct taataggttt tttgataata ttattagaag aaacattttg tcttccaatt
     5941 ttaactccat tagtagctga aactaggaaa agtgtatcta cgacatcact tgtaatactt
     6001 ggatttactg gaattattaa ctgaagcaaa tcttctctat tacctgattc tactgtaaat
     6061 gtacctgttt gactataaga acctcttaac tcatattgta ccgtaaagtt ttcccattgt
     6121 ggcacataaa tagaaacagg aatatccaaa gtatctccag caggaataac ttcttttgct
     6181 ccacttttaa aatgaatata aggtccataa tactctccaa gatcaacatt tcctaactca
     6241 tcattagaac atgataaggt aaaaacagta aataaaacta aaataatttt tgatatattt
     6301 ttcataactt aaatttttaa aggtcaaacc acatttttgt atctaaaggc ttatttggtt
     6361 tcgcatttgg attacttgat aattcagtag gagaatattg aagtcttcta ataatatctg
     6421 atagagaagc atcaagagga atagttagag caggatattc cgttcttctc cattgattcc
     6481 aaggctcaat tgttctatca aataagttta aatattgttg tttatggata gcctcaagag
     6541 catctgtaga agacaaagaa gtaagatctg ctagagtact aataaaagca gaagcctctc
     6601 ctggagcaac accataatgt ttcaaagaag catcaacacc acttaaatac ttagcatttg
     6661 catctccaga tgcaaatcct ctttggattg cttcagcgat taataattct tgctcggcaa
     6721 aagtatgcat gtaatctgga taacctccat taataatatc cgtactaaca taagaaattt
     6781 caggcactct atcagaaatc ggttcaaatg ggtctatacc tatgtaatca gatgcgtcat
     6841 ctccgatagt ataaaatttt tctaatcttg gatcactatc tgattgtaga atatccaaaa
     6901 ctacattaga agctaaccat gcctcttgtg tacttccata atatttttca acaatatacc
     6961 agattggatt ctttttacct gattcattca gatactgaaa cttccaatta tccgcagcag
     7021 aagtcatcat accacctgca tcaataagat ttccaatatc tgttgccttg cttggatccg
     7081 catcaaccat ggtcattaat actttaagtt tcaatgaacg agcgagtttt atccatttag
     7141 taatatcacc acgaaaccaa gggtctggta atttctctgt attagaaaca tctaaatctg
     7201 ctattgcctc atctaacaaa cctaaaatac cgttaaaaac gtctttttga ctatcatagt
     7261 gcggtgcact atttgtttct atttgaccaa cttctgaaaa tggaacatct tcccaaagca
     7321 tagtcgtatt gtaataaata aatgccttcc acacattaat ctgagctaca gcattttttg
     7381 gattaacaaa cttcttttta gacatctcta atgcttgttg agcattagtt aaggcatcag
     7441 tataagttgt actccaaaaa ttatttgtag tgtagggcga tacaataaaa atctcatcct
     7501 cccaataatt ataatcacca cctaaccaca tttgtgtata taatctagta ccataaactt
     7561 ccgccattct ttcagcggta taagcacctg cggagttatt taatagatat acaggatcta
     7621 ctgtggttgc tgcatcagga tctgtattta tatcaaaata accttttcta catgaagtaa
     7681 ctaaaagtac catcattata gaacatatta ataaagtttt ctttttcata tatagtattt
     7741 taacaattaa aatttcacgt ttaaattaaa accaattgat cttgtagatg gagaattaaa
     7801 aaattcatat ccctcaccta ttaagctagt tccaaaaaca ttactttctg gatcaacgtg
     7861 tggaacattt gattttaata atgctaaatt tctaccagat acgccaattg taaatccttt
     7921 aacggttttg ctattttcaa ataatttagc tggcaaagaa taacttaatg ctaattgtct
     7981 taatttaata taagatgcat caaatacatt tgcttctgtt atggaagttt gatagttatt
     8041 tttccaataa tcttccatac ttcttacagg aacatcgtta ggtctagtag ttccatcagg
     8101 attcaataaa acaccatcat caataatcgt tccatcacgg ttaatagccg tttctgctgc
     8161 taatcctgaa gttcttaagc ctgcagtagt tccgctatac attacaccac cttttctaaa
     8221 atcaaacaat acacttatcg ttaatccttt aatataaata tctgcaccaa gccccatatt
     8281 ccattttgga taaatatctc caaaacgttt aggcgaaggt gcttgttgtc ttagtccagt
     8341 tccagcatta ataatatatt tgccagttgc actatctctt aaataagctg taccatataa
     8401 accaaatgtt tgaccttcct ctgctttaat ttgaaatcca ctaaatccag aagttaaaac
     8461 atagtccttt aagccaggat ataattcttc tagtttttgt ttattcttag tgaaattcat
     8521 atttaaacca attgaataat cacgattttt agattcatat actgttgcag ttaccaataa
     8581 ctcatgtccc ttgtttgaaa cagaaccagc attgataatt ttagcgttaa acccagtaga
     8641 tgcactaatt ggtaaagaga tgatattatc tttagtaact ttttgataat aagaataatc
     8701 tattcttagt ttatttctaa agaaattaaa ctcacctcca acttcccaat cattttgtct
     8761 agaaggtttt aatgtttgag acggataagc actagttgtt tcatacccaa ctaaatcacc
     8821 atagggaaat gttaaattag taccaaattg tccaaaaact gagcttactg gagtataagt
     8881 gaagttagtt aaatatgctg aataagaaga acctacttgt gcgtatgacg ctctaagttt
     8941 aatataactc acccatttat cattataatt ttttattaat gataatggaa caaaactacc
     9001 actaactcca taatagttgt aaactctgtt ttccttagga agtgtagaat ttatgtcagt
     9061 tctaaaacta gcatcaatga aaaatgaatt ccaaagtcct aagtttaaat tgtgatatac
     9121 acctaataat cttgttttaa ctttagattc tgttgtagta atagttttag cattcgcata
     9181 agtatataac tgatctacta tcatatcttg tgcagcaaca tccaccaatc tacttgatac
     9241 atttctaata ttaagaccca ctaaataaga aacatcaatg tttttccatt tcttataaaa
     9301 cgtattaatt aaatctgaat tcaactgatt tcttacaata tctgttgttg aatatgctcc
     9361 tggaagcata cgtaaagtac cctttctcgt aattgcagtt ctattttctg aaatataatc
     9421 tgtaccaatt ctactagtaa atgtatacca gctcgtaggt ctaataatta cttctccagt
     9481 tccataaaat ctaaatcctt tatctgatac agggttattt tgagttatcc aatatggatt
     9541 atttacttga tttgatgcag ctccatctaa accaactaaa tttccaagtt catcaaaaac
     9601 atgattttta acatcattaa tatctacagt tctcggtaat cctaaaacta ttgaaggaat
     9661 aatgctttcg ttgtttgaac cttgtcttgt tcttccaaat gtattattgg ctactaatga
     9721 agcagatact ctagattcaa tgtatttatt tattttcttc cctgcattaa ttgtaaaagt
     9781 atgtcttcct aattttgaat taggaaggat tcctcgttgt gttacattac tataagcaaa
     9841 tctataatct ccagtttcat ctgcattgct taaagcaaaa tttgtaatat aggtcattcc
     9901 attttcaaag aaatctttaa cgttatttgg atttgctttt aaagttacag agtctccttt
     9961 aaaatcaagg aatttttgat tctgcactgc actaattttt ggaccccatc catttaaatt
    10021 tttcaaaaca tatacgccat tagttccttg agcatattca ttctgatagt taggcaatac
    10081 taaagcatgg tcagtaaata cgtttacgcc tacctcaacg gttggcactt ttgttttcga
    10141 tacagcacct ttttttgtgg ttataataat tgcaccatta gaagcctgag aaccataaag
    10201 tgctgttgca gcaccgcctt tcaaaacagt catcgtttca atatcatcag gattaatttc
    10261 actatactta ttaccttgat caacaccacc tcctaatatt tggtttctag ttcctgtatt
    10321 tcctaaatta gatacaggtg ttccatcaat tactaataat ggacttccac ctgatagtcc
    10381 actaacgcca cgaatcgtaa tttttgaacc agaaccaata gagcctgatt gcgatgaaat
    10441 gaccacccca ggtgtttttg cagataatgc atttaataag tttgtttctt ttaccttatt
    10501 taaggtttca ccacttactg ttgtagcaga ataaccaatt gatttctttt ctttctttac
    10561 agctaccgct gtaacaacaa cttcatctat cactgcttgg tctgttgcca aagcagcatt
    10621 aattactaaa gatgttattg gcttttccat agtagtataa cctacataag agaaaaccaa
    10681 acctgttatt tcttttggga cttcaagcgt gtatttccca tcaaagtctg ttattgtacc
    10741 aacaacagaa cctttggcag aaacactcac gccaataagg gcttcgcctg tagttgcatc
    10801 agtaactgta ccgcttatac ttctgttttg tgcgaacacc tgaactgaaa caatcagtcc
    10861 aagaatccat aatgctaatg ctctcttcat aaattaataa ttagtgtctt ttaatgttaa
    10921 aaatctctaa gaaattatac aaaatatttt tcaaggagca attatagtta attagtatta
    10981 aataaaaaaa tatttttttc atttttttta cgtcttagta atgtttttat aacatttctt
    11041 gatgtaggtc tatattatat actatttttt tgcatttctg tatagcaaat tgaagaatta
    11101 gaaactccct aagaaacaat tcgtttcatt tattttaata gaataatact ctttggtcaa
    11161 taaaagtaat gtattataca tttattaaat agataacaaa tcaattttac atacattttt
    11221 cacaaaagac ataaaaactt tatttagatt atatatcttt gcacatggaa aatatagctc
    11281 atctttctca caatcaaaaa tttgctggcg aaggtttaac ctttgatgat gttttgctta
    11341 tacctgctta ttctcaagta ttgcctcgcg aaacaaatat ctcttctaaa gtaactaaaa
    11401 atattgatat taatattcca gtgctttctg cagcaatgga tactgttaca gaagcagaat
    11461 tagcaattgc tttggcacgt gaaggcggta ttggtgtctt gcacaagaat atgtccatag
    11521 agcaacaagc actccaagtc agaaaagtaa aaagaacgga gagtggtttg attttagacc
    11581 ccatcacttt gcatccagat aataaggttg cagatgcatt ttctatcatg aaagaccata
    11641 aaattggtgg tattcctgta gtggataatc aaaatatttt agtcggaatt attacgaata
    11701 gagatttgcg attttgtaag gatcccaata tgttcataaa ggatttaatg actaaagaaa
    11761 atatcattac agctcctgaa aaaacaaatt taaaacaagc agaggaaatt ttacaccaat
    11821 acagaattga aaaactaccc atagttggga aagacaataa attggtaggt ttaattactt
    11881 atagagatat tatcaatgta aaaaattatc caaactcttg taaagatgag ggtggaagat
    11941 tgagagtagc cgccgcagtt ggtgttactc ctgatatgat gcaaagaatt gaagcattag
    12001 tagatggtgg ggtagatttt atttctatag atacggctca cggtcattca aaaggcgtaa
    12061 ttgatgctgt aaaaaatgta aaacaaactt tcaaaaactt agatgtagta gctggaaaca
    12121 ttgcaactgg tgctgcggca aaagcattgg cagaagctgg cgttgatgcg gtaaaagtgg
    12181 gcgttggtcc agggtcaatt tgcacgacta gaattgttgc cggtgtgggt gttcctcagt
    12241 taagtgctgt ttatgaagcg gctttagcat taaaaggaac gggtattcct gttattgccg
    12301 atggtggaat cagatatact ggagatatgg tcaaagcgat tgctgctggt gcaaatacaa
    12361 taatggctgg ttctttattt gctgggacag aagaaagccc tggagatact acaatttttg
    12421 aaggcagaaa attcaaagtt tatagaggaa tgggtagctt agaagccatg aacgatgggt
    12481 caaaagatag atattttcaa gatgtggaag aggatattaa aaaattagta cctgaaggta
    12541 ttgttggaag gattccatat aaaggaaaat taagcgaagt tgtttatcaa tatatcggtg
    12601 gattgaaagc tggaatgggc tattgtgggg caaaagatat tgctacatta caagaaactg
    12661 cacaatttgt acgcattaca tcagcgggtt cggtagaatc acatccacat gatgtttcaa
    12721 ttaccaacga ggctccaaat tatagcagat agtaatcgtt attaattcat ctttttctat
    12781 ttatatagaa attgaaagaa ttgattttct atttataatt taatgctttt tttgcttagt
    12841 aaatattacg cctatcattt ggtatttcat tcttatcttt gtaattatga gtaagttttt
    12901 aattgttgga ttaggaaata taggtaatga atatgctgac actcgacata atattggttt
    12961 taatattgtg gatactttgg cagatcagtt aaaagtgaat tttacagtag ataaattagc
    13021 aatgtatgct aatggaactt acaaaggcaa acaaatccat attattaagc caactacgta
    13081 tatgaattta agtggaaagg caataaaata ttgggctgaa tttttgaaaa ttgacttcga
    13141 aaatatttta gtaatccttg atgatttagc attaccattt ggcacattac gtttaaaatc
    13201 aaaaggctca gatgcaggac ataatggttt acgttctatt caaactgaat tgcaaacaca
    13261 aaactatcca agacttagat ttggaattgg tgataatttt cagaaaggca aacaagtgaa
    13321 ttttgtgctt ggaaaatgga gtgtggaaga acagaaaaaa ctagaagata ggattttatt
    13381 ggcttgtgaa atgacacttt ctttttgttt tgctgggatt aataatacca tgaatagtta
    13441 taacaataag taaacttttt tatcttagca tcaatatgtt agaattattc caaattttct
    13501 tcaaccacaa aaagtatatt ttaggcttta cttttttagc cattctatta agtattatta
    13561 ttactattcc agcgataatt ccacctaaat ttcagtctaa aactattttt tatgttgcca
    13621 accctttgag tacagacaga gctaatttat ttaatcaaaa agatggtgga attgtttctc
    13681 aatttggcga ccgaaatgat gccgatagat ttattgcaat atctacttct tctaatgttt
    13741 tagatgctat tattaaagat tttaatttaa tcaaacatta taaaatcaat aaagaaaatc
    13801 ataatttgaa tttatatttt gctaaaaaaa agctacttaa aaacttaaat ataaaaagaa
    13861 atgataacga tgcaatagaa attacttgct tggacacgga ccctgaaatt gcagcaaaaa
    13921 ttatacaaaa tttgatacaa caaaccaact ccaattactc aaaaatcaat aaagacaata
    13981 aacagaaagt attaacagaa ttagaagtat taattaaatc aaaaaaggaa gccctcactc
    14041 aactttcagg gattgagaaa acaactgctt tagaatctat attagaatta gaaaatctta
    14101 aaagccaata cctagtttca ctcaacgata attttgcttc tatttatgtt attgatgctg
    14161 ctgaagttgc agtcaagaaa tcgtatccta tacgttggca aattgtttta ttaactgctt
    14221 tttttgcgtt tgcaacggct ttactttttg ctatcgttta tcaattgtat gaacaacatg
    14281 caaaagcaac taaggtataa tattatcgaa tacttatctg cttacaaaaa gcgactaagt
    14341 attttgttta tgttttttac attactgttt ttttctggtg caatttactt caacgaacct
    14401 tttttatttg catttccatt tacattgctt gttatttctt tgttcatttt aaagattgaa
    14461 tcgctttatt ttttattgct acttgtaatc cctttttcta tagaatacga ttttagtaat
    14521 agtattatcg atttgccatc agaaccttta ttaattttat tcgctgtgct tttaagtatt
    14581 tattgtttat taaactacaa aaaagttcta gaaaaaataa attataatcc actagtttat
    14641 atcattgcta tacatttggt ttggacttat ttttctacta ttttctcaac aaatattata
    14701 atttcattga aaactagcat cgtaaaaacc ttatatcttt tagtatttta tcttggtacg
    14761 gctttattca tcaatcagtt taataaaatt aaaaaattaa tttgggtact ttggatacca
    14821 acatttttga caatcgttta tgcattaatc attcattctt cttataattt ttcatttgaa
    14881 agtatcaata aagcattgca tcctatctat agaaaccatg taaattatgg tgtttttatt
    14941 actatgcttt ttcccttttt aatttttcta aatagacagt ccaaagctca aactattcca
    15001 aaattattta cgcaaatatc cataccaata accttagttg ctatatattt tacatatact
    15061 cgtggtgctt ggttaggttt attaagcatg cctatttatt atattgttct aaaattaaaa
    15121 tggacaaagt ctatcctaat tattgtaagt atcacaattt taagctttgg ttcttatcta
    15181 ttttataata ataactacca aaaatttgca cccaatttta ataagactat ctaccatgat
    15241 aaattatctg cacatctaaa ttctactttt gaaatggaag atatgagtac tgtggaacga
    15301 ttttatagat ggttggcatc tataaaaatg agcaagaaaa atctaattta tggtgttggg
    15361 ccaggaaact ttactgaaaa ctataagcct tatacagttg catcttatag aacgtatatc
    15421 agtgaaaatg aagaaaaatc aacggtacac aattactttc ttttaatgtt ggctgaacaa
    15481 ggtttggttg gattacttat ctacgttgct tttgtgtttt atcttttcat atatattgaa
    15541 aaattatacc ataagcaaat taagaaagaa cataaagttg cagtattgtt aattgcttgc
    15601 agtattttag cttttttatt caacaataca ttaagtgatt ttttggaaac aaataaagtt
    15661 ggttctttgt tttggataaa tattggacta ttaactattt tttcaaatcc aattaaggaa
    15721 aatgagcatt agactaaaat ttcaaacgat tgaaactaat aaaaatgaag ctcattatgt
    15781 ttcagaacat gatgctgaaa tcatttttaa tttcttaaaa aaaacaaaaa ccattcaacg
    15841 tactttacaa ataggtttgg gtgatggtgc tttagctttt gttgttttat cctttacaaa
    15901 taaattgcac attgcaatcg acccagacca atcaaaagtt caatttgaag gaattaaaaa
    15961 tttaaaacga tttgaattat ttgatttagt ggactttcgt gatgattata gctcagcggt
    16021 cttgcctaaa ttattagaag gtaatgtctt gccagatttg actattttgg ataatgacca
    16081 aacatttgat aaaatgataa atgattttgt aaatattgat cttatttgta aaaagggaag
    16141 tattatactt attcgaaaat caaaaaatcc aaacaattac cttttacatc aatatattac
    16201 aaaaaataga gtagattatt ctattgttga attagaatta gaacactttg tactctatga
    16261 gaaaattgct agagatatta gaagtatcaa tgattttcaa gggataaatt gctaatctat
    16321 caactcaaaa ctatgtgtta gctctgcaga cttttctaac atttttgaaa cgctgcaata
    16381 tttatccata gaaagctgaa tggctttttc ggcattctcc ttttttatta tacctgataa
    16441 aatataatga acatgaattt ttgtaaaaac tttaggttca gtctcagctc tttctgcctc
    16501 gacaattact ttataacttt ttacgttttc tctcattttt ttcagaatag ataaaacatc
    16561 cattgaagaa caaccaccta aagccattaa aaccatttgc attggtcttg cacctagatt
    16621 ctcaccacca atttcaggcg aaccatccat tttaacaatg attccatcat cattttttgc
    16681 ttctaaataa actgcattat tctttctttc taattctatt ttcattgctt attattttac
    16741 tttttcaacc attatgccta catcttttac acccaacaac ggctcttgtc tcattagatg
    16801 aacgactttt acgatataga ttcctgattt tttaaaaata taattttttt ggataggata
    16861 gatttgtgta caaacacttc cagtgcattc tccataaggt ttgccaattt tatcaaacaa
    16921 aataacttct tgcattttgc cttcttttcc tttttcaata atatttccaa aaagtttaaa
    16981 ccaaatatta tcaaattcat agcctttgtt gtatcgaacg cctaaattga tattatacgg
    17041 tgtattggtt tcttcaattt taactttaaa tgttagtgtg tcatttgcgt tccaaccgtt
    17101 ttcatttacg ctttcatatt tagcaaattc atattttgaa gaacatgcaa caataaaaca
    17161 gatagaaagc agcgtcagaa aatatttagt cattttgaaa ttctttaaag ttaaacaatg
    17221 tttttagagc aatgtttgat acaattgata aatttgttcc gttatttttt ctggttgaaa
    17281 tttttgaact tgtgcagcag ctttatatct aatttcatct gccaattcta aattataata
    17341 aataaaattt attgcattag acaattcctc cacatttttg ggatctataa aaatacaagc
    17401 atcgccacca acttcaggca ttgatgaaat attgctggta ataacggatt tgccacaaac
    17461 taaagcttct aaaattggca aaccaaaacc ttcatataaa ctcggataga ttaagccttg
    17521 tgaaatgtga taaataattg gtaattcata ccacataata ttatttaaaa agaaaattcg
    17581 attctccatt ttcttttcat aaataaaaga ttgggcttct tgatattcat cgcctccatt
    17641 tgatacgaca actaaattct tatcttgatt ttcaggcaaa gccattgctt ttagaagcgt
    17701 tataaaattc ttgcgtttag aaatgctacc cacatataga acaaaatccc ttggcaaatc
    17761 atactttaca ataaactctt ctttcaaagc ataagagcaa tcatgttcgt attctttatt
    17821 ccacgttgga taaattaaat ggattttact ttcttccact ttaaaaagct caaggatatc
    17881 ctctttggtc tgttggctca ctgcgataac agcatctgca ttttctagtg ctttttgagt
    17941 tttatattta taaatatttc tatcaaaaat agagaatgtt ttaggcagtt ttttgaagat
    18001 aacatcatga atagtaacga ccttttttac ctcttttttt aatgaaggca attcattact
    18061 taatccatgg aaaatttgaa tattattgga agaaacatct tctactattg ccttttgtcg
    18121 ccaaatttta tcgctgtaat gttctgaaac tacagtgttt ttagtaaaat aagcatattt
    18181 aaatggtgtg gtatcaaaat attttttatg gacaaacaac ttataatttt catcagggta
    18241 aaattcaact aatcgattaa ataaagttcg gctatacgtt cccaatcctg ttggattggc
    18301 atataatctc ttggcatcaa aacctatatt cattcttaaa gtaaagatag atatttattg
    18361 atttttttag acattttttt tcattccatc ccaacccatt gcaataatat catgataatt
    18421 tccacctttg gtatgtaatt ttattcctaa aagtgcatca gttatctctc caattatagc
    18481 aatatcatga tttttatcta caattgcttt atcttttgga ttaatggtaa acaacaactc
    18541 ataatcttcg ccgccattta atgcacaagt aattggatca atatttaaag ataatgctgt
    18601 atcataagtt tcattggcaa ttggaacatg ctcttcttcg agtgtacaac ctacattact
    18661 ttgttgacaa atatgtaata tttccgaact caagccatct gaaatatcta tcatacttgt
    18721 cggtactaat tgttccgtat caaaaaattg aataatatct tttcttgctt caggctttaa
    18781 ttgtctttcg acgatataag taaatttatc cagttctatg gaggcttcag gtgcattcaa
    18841 aaaaacttgt ttttctcttt gcaagatttt taagcccata tatgcagcac ctaaatctcc
    18901 tgaaacgcat aataaatcac cttctttagc accatttcta taagctattt tttcaaaact
    18961 ttgttcgcca attgccgtaa ctgaaattac taagccttgt ggagaagaag ttgtatcgcc
    19021 accaacaata tcaacttgat acctctgaca agccaatttc ataccagcat aaatctcatc
    19081 gatggcttcc actgaaaatc tattagagat tccaaagctt actaaaactt gttttggaaa
    19141 tgcattcatt gcataaacat ctgaaagatt gaccattatg gctttatagc ctaaatgttt
    19201 taatggtgta taaactaaat caaaatgaat gccttcaatt aataaatcag ttgtcaaaac
    19261 ttgatgttgt gcggagttca caactgctgc atcatcgcca atacctttaa tacttgaagc
    19321 attatatgta gcaatatctt tagttactct atctattaat ccaaattcac caattttgct
    19381 aatttcagtt ctactttcca taatgcaaag atacttttct ctacatattt ttgagttata
    19441 agataaaata ttattttagc atttaaagtt tatgaataat taagtcccca aagtttatga
    19501 atataggaat tgtagccaat acctctttta atatctacaa tttcagattg ccattgatgc
    19561 atttcttaaa agctaaagga catcatatta ttgctattgc tccaaaagat aattttacag
    19621 agaaaattat tgctgaagat tttccttttg ttgaaatcaa aacattaagt cgcaaaggca
    19681 ccaatccaat acaagattta aaactaattc ttgagctaaa aaaaatctac caacaaacaa
    19741 atttggatat cgtattgcaa tacactatca agccaaatat ttatggaagt ttcgcagcaa
    19801 aattggcaaa catcaaatgc atttgtacgg taacaggatt aggctacact tttctaaata
    19861 atagcattgc ctcaaaagtt tctaaatggc tatatcgaac agcgttttat ttctcagata
    19921 taattttatt tcagaatgaa gacgataaag cgacttttct aaatttaaaa ttagcaaatg
    19981 aacgaaaaat aaaaattgtt cctggttcag gaattgatat ctatcgattt gataaaagct
    20041 tttgcacaaa tacaataact gatacaaatg ttgtattttt atttattggt cgcttattaa
    20101 aagataaagg aatttttgaa tttattgaag ctgcgaaaaa aattattcag caatacccca
    20161 agacattatt tttgattgta ggcgaaattg accagcaaaa tcctagttct atcagccaac
    20221 aagaattaga aaaaattaca cttattccaa atattaaata cttaggattt ttgcaagaca
    20281 caagagtcgc tatatgtcag gcaacttgtg tggttctgcc ttcttatcgt gaaggtttac
    20341 caagagtaat tttagaagca atggcgatgt caaagccatg cattacaaca aatgttcctg
    20401 gttgtaaaga tgcagttgac tccagttgtg cttttttagc agaagtagaa aacagcgaag
    20461 atttaaaatt acaaatggaa aaatttattg tattagacaa tcaaaagaaa ttagaaatgg
    20521 gtacaaatgc aagaagaaga acagaaacaa tattttctgc taatataatt gcagaaactt
    20581 attttaaatt aatttcaagt ttagttaaga aatgattaaa aaataaaaaa atagttttta
    20641 tatttgaata cacaatgcat aaaacagagt ttataattcc tgctgctata ctttcgtcat
    20701 tccttttgac ctattcattt attccttcaa ttataaaaat tgcagaaata aaacatcttt
    20761 atgataatcc agatgagcgt aaacttcatg gttgttctac accaaattta ggtggtattg
    20821 caattttcag tggctttttg atttcttttt gtttattttc tgttatagat agtgcttatc
    20881 aaattcgtcc aatcattgcg gctttatgtt tagtattttt aattggagca aaagatgata
    20941 tcgttccatt agttccatac aaaaaattta ttggacaaat ttttgctgct gcaatcgttg
    21001 tggttatagg aaaagtgaga atttctagta tgtacggact gtttggcatt tctatgctca
    21061 atgattatgt cagtatttta ttaacaatta tcaccattgt atttatcata aatgcattaa
    21121 atattattga tggcatagac cttttagctg gtggtgttgg ttttattgca gcaagtactt
    21181 tcgccgtttg gttttattat tatggattta taaattatgc tgtaatgggt gcagcacttg
    21241 ctggtgctat tttagcattt ttaggattta attatagtcc tgcaaaaata tttatgggcg
    21301 attcggggag tttaaccatt ggtctgttat tgtccctaat ggcaatagaa tttattgaaa
    21361 aaaatgaagt tgttttaaga agtggaaact ctgaaggaat agccatttta tcttctcctt
    21421 ctatggcaat tgcggtttta attattccta tatttgatac attgcgtgca tttgcattgc
    21481 gtattcttaa agggaaatct ccattttacg cagatagaat tcatattcac catagattag
    21541 tggacttggg gctaacacac attcatgcgt cttttgtgct ttttgctttt aatattcttt
    21601 ttattgtaag tgtttatttc ttacaaagtc taggaaatat gactttatta attggagaat
    21661 ttgccatagg ttctttattt acctttcttt tattctccat aaaaaaaaag aaaaaatcca
    21721 atagcactat tcataaaaaa gcacaaaact taatagaaac ctactaggat ttattcctta
    21781 tttggaatct tactcattgc tctttcagaa attgcagtaa tactcaaact tgggtttaca
    21841 ccagggtttg ctgaaatcat agcaccatca caaaccatca tattcttgta attaaacaca
    21901 aaattatctt tgtcaattac gccttcttga gcattatttc ccatacaagc accacctaag
    21961 atatgtgcag tggtaggaat tccaaataaa gtttctgtac ttaaagtcat tgcttttcca
    22021 tttattattt cagcaacatg attggctaat tcttgagctt ttgggttgaa tgcagttgga
    22081 gctggacctg tatctaaatt agattttaaa ccacctaaaa tatttctttt aaaactcaaa
    22141 gtagaatcaa tactttccat atataaaagt atttggctac gtttgctcca atcatcaata
    22201 aaagctgctt ttaagttcgt aattggatgc acaaataaat cttttaccat tctataaaat
    22261 cgagtaaaaa tatttcctcc ttgaaccatc ggagccataa aaattctcca gaaaccagaa
    22321 ccatcagaat attttactgg ttctatatgt cggctttcat caatatttat gattgaacca
    22381 atagcaattc ctttggaaaa aacagcatct ttgtcaaaac ttgtaacacc aatcaaactt
    22441 tcagaattgg tacgaatgcc cgttcctact ttatcagaca gattgggtag agatttttct
    22501 tttaatttta acaacaaatc aaccgttcct aaaactcctc ctgaaaaaat aacacttttt
    22561 gtttttacgc tatctttttt tggaaaataa ctaagtgagt ctttaaaatg aatttcataa
    22621 ccatctgcac catcaatact taaaggaata acatcaaaaa cttctttttc tgcaatgact
    22681 ttaacaccca attgttcggc taaatataaa tagtttttat ccaaagtatt tttggcatta
    22741 taacgacagc ctaacataca accgccacat tgattacacc ctgttctagt tggtcctttt
    22801 ccatcaaaat atggatcagg tacagtaaca tcttgtttgc caaaataaac agctacttcc
    22861 gttttatcaa aggcttctgg ctttccaatt ctttctgcca attccttcat tactttttca
    22921 gaaatactcg tatatggatg ttttgttgct cccaacattt tctttgctaa tgcgtaaaaa
    22981 ggttttaatt cttcttccca attttctaaa ccattccaat gtcctgagtt gaaaaattct
    23041 gattttggaa caggtaatgt attggcataa actaatgaac caccaccaac accaacgcca
    23101 cttaatacgg caacatgtct aaaaaaggtg agtttaaaca atccttgcat acctaatttt
    23161 ggcatccaca tccattttct taaattccaa tttgtttttg gaaaatcttc agcacctaat
    23221 ctttttcctt tttcaattac taaaactttg tatccttttt cagctaaacg tagtgcagaa
    23281 acagaacctc caaagccact tccaataatt acgtaatcgt attctaaatt tgccatagtt
    23341 ggaatttatt tatagtaaaa gtaaataaaa aaggagaaag tttaatgata ctttctcctt
    23401 tttatcttta gttagttcag ttgattttta ttccattccc attggagttc catagtaaac
    23461 atctacgcca taacctaatc gaatttgact ataatcaatc caatatgccc aatctttttg
    23521 ctttctcaat ctattataag tattgaatcc attcatagca atatctactc tatgaacaat
    23581 atttactaaa ttataagagc cttttggaat tttaatatta tagccttttt gtggttcagt
    23641 atatttattt gctcctataa aaatttctcc aaaaatacca tattgaccgt ttgtcatttt
    23701 taagaccatt tcatttaatt gtcccatcaa tcctaaaagt ggtttatccc aaagtggttg
    23761 caaaatatag gtttgttcat gaataccaaa ttgaatagct gctagtgttt gcaaatcttg
    23821 ataagcctct ttttgtccgc atcttaaagc ttgttctgct tgtttttgga acttaaatcc
    23881 tttaattgct tcaggtgtta gccaagcaga ctcactgtct aaatctctat ccacatattt
    23941 attcagtaac accaaagctc caatatcaac aactacattc aattgtcctt ctaatagtaa
    24001 aactgtagcg tcagttaaca tttgtccaat attttgagtc gtatctttta atggaatttt
    24061 gatattattt ttttctaaat aatttacaat ttgagaagct aatactacac ctcctcttac
    24121 ctcgtttgct gcaaaaattc ctagattcgt ccacatcatt tctggaacat ttctttgtaa
    24181 tgcatcgtaa gtcattaaga caaaagagtt ttttgccata gtcgtataag caacagaacc
    24241 attataatgc actaaggctt tcgcctcaac ttcattggct ctcgaacgta tattatgttt
    24301 taattgttca tatttttctt ccaaagtcat atcattagta acaccattgt acaaacgagg
    24361 aatgtcattt gcaattactc taatatctcc aaaaaatgct ctctcttccg cagaaatttc
    24421 tgttgttggt gtcgtttttt tggcgacctc ttttaaaagt tcttgttgtt gtgcttcggt
    24481 taaattggca ttcaaagcac tagacaaatc gcattcttgt tgtttttctt ttttacatga
    24541 taagaatgta aaaacaagaa taaatagtaa aaaatattta ttcattaaga ttggttaaat
    24601 aaatttgaca atcattgttt tttcaacttc atcttctaat tcctctggag tatatgcatc
    24661 attctcaaat atgaattttg taactaaatt attaggaatt tcttctaaat ggacatagtt
    24721 tacattgata tttgaaggag catctttcca aatctccact ataggttttt tattcttccc
    24781 aataaacata tctaccactg aagttggaaa atatttttta tttaagattt ttctagtatc
    24841 cgataaaaca tatactggct tatcaaaata ctttgcaata agacataaat taaaggagcc
    24901 tgtttttcca ataaaagttt catgcattgc aatttcattg cccattaaaa cgacatcaac
    24961 atcttggata taattggcaa tagccgaatc atcaataaca gttacgggaa ttccaatttc
    25021 tgcaattttt gaggcttgtt gttttccaat tttatcgtgt aaactaatga tttgaataat
    25081 attacaagtt tttaaattga cgttcaagtt ttcaaccaaa gcagtaatgg caatatcatt
    25141 accatgcacc aaaattgttt tatcagcaaa atcataagtt ttaagtgcaa cttctgctgt
    25201 ttttctatct acatttttcc atcgttcatg aaagacctcc aacaattctt ttataataat
    25261 gggttgattt tctggagaaa tttgcaacaa aaaatgattg ataaaatgat tgattgaatt
    25321 aatatttggc ttttctgtca gaatggtttt taacccttgt aacatttctc ttacagatgc
    25381 agagacttta ttgtcagaga ttaaagaaat aagcttaccc gtagttttaa tggcttgttg
    25441 agcattattt acactctcaa ataatttgtt ttctgtaagc tttttaaaaa gtcccatact
    25501 atcttaatgc aaaaatacga atttagttgc taacttttct ttcttgtcaa ttcctttgca
    25561 aagtactaaa taaacgccac ttgccgcttt acctccgtta attaattttc catcccacgt
    25621 tgcttgtcca ccagttgcct gtgtttcata aactaaatta cctacagcat cagttatttt
    25681 cacattacaa ttatttggaa tgtttttaat agtaattatt ccttgataat ttgattgaac
    25741 tgggttggga taaacaaaag catttccact ttcagaggta gtttctgttg ccgtacttct
    25801 ataagagcaa atacctaaat ttgttccgat aaaaacatct ccagtattgg gttgtatggc
    25861 aattgaacga atttcgtttc ctaaaagtgg gctattatct ttattaaaat aatgaatttg
    25921 tgtttctcca tcttcactta ccaaatatat tccattattg gttccaatcc atttccgatt
    25981 ggctgcatct actttgataa ctgtaaccgt ttggtcttcc aataaattat cacaaaaatc
    26041 tgtaccatca tttctgccaa tacagatttg ttccgcaggg cagtttctat ctattccaga
    26101 gcctgcacag cgtataacgg cgataccttg cgaagtgcct aaccaaattt ctccattatg
    26161 gtctttttcc aaacacaata tattgtttga tggtaaattt ccgttgtttt ctccaactga
    26221 atagtttaca tattgatcgt ctgaaatatc atcaatggta taattatggt taaaaacagt
    26281 tagccctcca gatgaactat ttttcgaaat ccaaatttga ccataatcat cttccacaac
    26341 atctgtcaat atggtattat ttaaaaatac attattgaag aatttataat ttccgtctgt
    26401 ttttctacaa attaatggaa aagaactata agcatcggtc agccacacat tatttgctgc
    26461 atcaacagtt gagtttgtaa ttctaaattt ttgagttgag tttgggcgat acactttagg
    26521 tacaattaca gaattatctg atacattcat ttcaacaata cctccaccag gcatatttcc
    26581 gcccaaaata attttatttt ctgctgtcaa tggttgggca atagtaatat cataaatggt
    26641 atcaaacaat tgccaattat attgtccata acttgtccaa attccctctt tataaatata
    26701 tattccattt ttattaaaag ttggattggc atttgaacct gcattggaag acgctacata
    26761 caaagtattg ccaataaaat tcatatcttt attggtaatg gcacttggac catttggaat
    26821 aaaacgttga gcatttggag tatcttttat taaaccatca tataaatcgg catgccatat
    26881 attaccattt ttatcttcta aaacttctaa tggaaaacga atattgctgt ttggagcgag
    26941 aaagctaaaa gtattatttt cccattttcc aattcgctga ctactattgt ctgttttgtg
    27001 ctgtactaaa tataaatggt tgttatctac tgataaatca atggttttcc aattcagctc
    27061 attgaaaacc aaagcccaat tagtgttatc ataattataa atttgatgat ctttagctac
    27121 ccaaatctta ttgtcaaatt ttgcaattgc agaaatattc ccgccagtat ttccatcaga
    27181 tattttttgc caagtactaa aatttaaaat caaaggattg ttcaactctg tttgaaaaat
    27241 accattttcc gtagcaacat aaatctttga attatctaaa gctataaaat tacaagccaa
    27301 tggatttgaa ccatcaaaaa agttgtatgt ttccttgatt tctttcttaa ttaaatcaac
    27361 cacaacaaca ccaaatccaa ctgcaaaata agccaaacta tctttggtag caatatttct
    27421 taccgtttta ctactgataa tatttgcatt tttcagataa ggcaaattgg taatctttcc
    27481 attttgaatt aaatcaatat tggcattttt ataaacaatt atcaaaacac tatattcttt
    27541 attataggca attttttgtg tttctacttc agacaaacca tcacttttgg tataggtttc
    27601 aataaaatga ttatcaacat ctacactaaa aacaccatac tctgttgctg cgtagatgtt
    27661 ttggttgctc tgtgtaaagg aaacaacgtt tgacaaaggc ataaacgacc gccaagttcc
    27721 aagcggcaaa ttttgtgctt gtacaaaaat tacaatacta acaagcaaaa taaaagtaga
    27781 tatccgtttt aacataataa gctgtcttgt ataaaagtac atattattat tttatttttc
    27841 tttaagtttg agctgcaaaa taagaagaat gagagtagtc atacagcgag taaaaaatgc
    27901 ttccgtttct atagatgcta aaatagtagg acaaattgat aaaggactat tggttttatt
    27961 gggaattgaa aataatgata cggacaaaga tgtagagtgg atttttaaaa aaattgtcca
    28021 aatgagaata ttttcagatg aggctgacaa gatgaactta tccattcaag atattggtgg
    28081 aaatatatta ttaatctcac aatttacatt atttgcttcc actaaaaaag gaaatcgacc
    28141 aggttttaca gaagccgcac gaccagaaaa atcaattcct ttatatgaga aatgtatcgc
    28201 taccttaaat acattaatag gaaaaaaaat tgaaactggc atgtttggtg cagatatgca
    28261 agttcaatta ataaacgatg gcccagtaac aataattatg gatagtaaac gaccagaata
    28321 atggaaatta aaatacatag taatttaatc aatgtcataa ggcaaacttt agaactgatt
    28381 tttaatcaaa ataaaatagc agataaggaa ataccaaaag ctctgaaaaa taaacaatat
    28441 ggctcgaaag acagagcatt tttggcggaa acaatgtatg atattgtacg ctggaaatta
    28501 aaatacga
//
LOCUS       DAOQCW010000018        26547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_678_length_26547_cov_5.340329, whole genome shotgun sequence.
ACCESSION   DAOQCW010000018 DAOQCW010000000
VERSION     DAOQCW010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 26547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26547
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_678_length_26547_cov_5.340329"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="PLS16_02900"
     CDS             <1..590
                     /locus_tag="PLS16_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNJ60057.1"
                     /translation="TNGVKQQYLVAIGFLMFFIYSFWGYKILTPDTGEQDFFWLLIVR
                     GFGLSLLFIPITTLALSSLKGKEIGEGSAFTGMMRQLGGSFGIAVITTFVANISTKNV
                     ALLSEHLNVNDLDVQQRIEMMKHGFMAKGMTPDVALQAVYKALDITVQKQATVIAYMD
                     VFLYLGILFLICIPFVLLMKQKKGAAPIDMSEAMH"
     gene            841..3423
                     /gene="gyrA"
                     /locus_tag="PLS16_02905"
     CDS             841..3423
                     /gene="gyrA"
                     /locus_tag="PLS16_02905"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNJ60058.1"
                     /translation="MSEELEGQDSRIVPINIEDQMKSAYIDYSMSVIVSRALPDVRDG
                     LKPVHRRVLYGMQDLGLASNKPHKKSARIVGEVLGKYHPHGDASVYDTMVRMAQDWSL
                     RYPLVDGQGNFGSIDGDPPAAMRYTEARFRKIAEEILQDIDKDTVDFQLNFDDSLKEP
                     TVMPTRIPQLLVNGASGIAVGMATNMLPHNLSEVVDGIVAYIDNNAIEIEELIKYVKA
                     PDFPTGGIIYGYEGIKEAFLTGRGKVRLRGRATIEENKDGRTQIIISEIPYQVQPKMI
                     TTRAAELVNDKKIDGIAEIRDESDKDGLRIVFDLKRDAIPNVVLNNLYNFTPLQSSYG
                     INNIALVAGRPQVLNLKDLIRHFVDFRMEVVIRRTQFELNEAEKRAHILEGFMIVLEN
                     AAITDEVIALIRKSKNPEAAKDALMERFGLSEIQCKAILALQLSRLTGMEIEKIKEEY
                     RQIMELIGKLKDILDKEALRFEIIKEEILEIKRKYGDERRSEIVYYGDDISYEDMIPE
                     EEMVVTISHLGYVKRTSLDEYKKQNRGGKGSFGGKTRDEDFIEHIFVASTHNYLLLFT
                     ELGKCFWLKVYEVPEGSKTAKGRPIQNVINIPQEDKIKAFINVADLKNEELLDSHFII
                     MATKKGTVKKTSLREYSRPRQNGVNAITVREGDELVSADITNGKCEIILGSKAGKAVR
                     FEEADARAIGRTASGVRGITLKDEADELIGMVCIDTSSELTPDIMVVSENGYGKRSLL
                     EDYRKTARGGKGVTTLNITDKTGELIAINAVTDDNDLMIINKSGLTIRIGLDTIRTMG
                     RNTQGVSLIKLKENDAIAAVTKIAHEEEEEETAENGTDIDNNSIENNSNELNENSNIE
                     NKED"
     gene            3425..4720
                     /locus_tag="PLS16_02910"
     CDS             3425..4720
                     /locus_tag="PLS16_02910"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ60059.1"
                     /translation="MKKITLVLIGVLLVNVIFAQKGKVNSASYALSEGKANEAKQFID
                     EALTDAETQQNAKAWQIKGDVYKNIYEGKVFFAQTPNALEDAKAAYLKAYELEVNPKK
                     KGVVAPSLEHLQGYFFNEGLSRFQDEKWTEAYMKFSEAVNINEFLLANNLAKTIDSGA
                     YFATAVAANNAGLLDKAKPLLEKLVALKYNNKAIYESLADIYAKEGNPNFMNIVNEGL
                     KKYPDSKTLQVSRLNYYIENGKTDEAIAEIQNALNADPKNYLLLFNMAVLQEQLGNYD
                     DAKIYYEKAIAAKPDFMDAYFNAGALYYNKAIEINKVLVKDDAASIDSTLAEFASKDV
                     ISIDDIRNALKDPNMGALLNSFQNYSGDKATLKDKVSKIKENIKYDLLIAKRNALFNK
                     TLPFFEKAYSIQKEDQLKSALKEIYARMNMFDKVKALDQ"
     gene            complement(4836..5951)
                     /locus_tag="PLS16_02915"
     CDS             complement(4836..5951)
                     /locus_tag="PLS16_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HNJ60060.1"
                     /translation="MSQINPVQTAEILLMGPGPSNSMPGVLQAMSEPVLSHLDPDFLK
                     IMDEIKEHLQYLFVTQSPHTYVVSGPGSLGMETCFVNLVESGDKIICCHNGYFGDRMH
                     ENAKRYGAEIISVTAEWGKPISLDDVENAFNTNINVKLISVVHAETSTGVKNDVEAIA
                     KLAKKHGALIIVDAVTALGAIPLKVDEWGLDAVYSCSQKGIGSVPGMSPVTFSQAAFD
                     KVLNRKNPVPSWFQDITVYEKYWATQTARPYHHTAPSQQYYAILAALRHIKSIGLEKI
                     WNQHIEISAYFFQEMTSRGWKFFVEGDAKLPQLNTVLLPEGFDDASNRTKMRNEFKVE
                     VGAGLGSMFGKLWRIGIMGGNVNKEAVDQLLKAIDNL"
     gene            6298..8907
                     /gene="mutS"
                     /locus_tag="PLS16_02920"
     CDS             6298..8907
                     /gene="mutS"
                     /locus_tag="PLS16_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNJ60061.1"
                     /translation="MSKPKEVKETPLMQQYNKIKQTYPDAILLFRVGDFYETFGSDAV
                     KAAEILGIVLTKRANGSQTDLELAGFPHHSVDTYLPKLVKAGYRVAICEQLEDPKMTK
                     TIVKRGVTELITPGVTVNDKILDHKANNFLASVFYENDAMGIAFLDISTGEFFVAQGN
                     LDYLDKLFQSYTPSEVVFPKFQQKKFNSTYANKFYTYAIDDWVYQDDYLEKKLLNHFQ
                     VQTLKGFGLDNLALGIKAAGACLQYLNEAEQKSLDHITQIQRIENNHFVWLDRFTARN
                     LELVHSMHEKGVTLLNIIDKTVAPMGSRLMRRWVAMPLKNKVTIDERLQAVEYLIKEQ
                     DNSNKLSQSIKTIGDLERIISKVSLLKISPRELNQLKKALSSIADIQILCQQSENITL
                     KKIGEQINLCSSVKDKIEVTITDDAPVAIQKGNAVKIGISEELDTLRAISLSGKDYLT
                     KLQNTEIEKTGISSLKIGFNNVFGYFLEVRNTHKEKVPETWIRKQTLVSAERYITPEL
                     KEYEEKILGAEEKIQKIEEQIFTELIHYIIDYIKPIQLNAQLVAQLDCLLSFATVALK
                     NNYRKPIITEDNILKIKEGRHPVIEQQLPLGEQYIPNDVFLDNESQQIIIITGPNMSG
                     KSAILRQTALIVLLAQMGCFVPAQSAEIGIIDKLFTRVGASDNLSLGESTFMVEMIET
                     ASIINNISEKSLVLLDEIGRGTSTYDGISIAWSLVEYLHNNKTARPKTMFATHYHELN
                     ELADKFERIRNYNVAVQETGNKVLFLRKLVEGGSGHSFGIHVAKMAGLPNAIINRANE
                     ILGELESNRSISSDISKTLKKLPENQNFQLNMFSMQDPRLLKLKEVIEQVDINTMTPV
                     EALLKLSELRKLL"
     gene            complement(8899..>9303)
                     /locus_tag="PLS16_02925"
     CDS             complement(8899..>9303)
                     /locus_tag="PLS16_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNJ60062.1"
                     /translation="NWELYKKVDFFKPNAIEFDAMTHLLKIDELSIEEQLLYLKNYLQ
                     VENLFITLGKDGSISMNEQNKIHRQAIHQSIEKVDVCGAGDSVIAMLCLSNLSNNSMQ
                     AIAYLSNLAGYIACSHAHIYAVKFEDITKLLK"
     assembly_gap    9304..9403
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9404..9687)
                     /locus_tag="PLS16_02930"
     CDS             complement(<9404..9687)
                     /locus_tag="PLS16_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60063.1"
                     /translation="MELEINLNFEDKRILVIGDIILDKFVYAEYAKMSPEYAEVPVLK
                     VEKCSYCLGGAANVASNIKTMGATPYLVGIVGQTYEPEFKNIETLMKISS"
     gene            complement(9766..10809)
                     /gene="ilvC"
                     /locus_tag="PLS16_02935"
     CDS             complement(9766..10809)
                     /gene="ilvC"
                     /locus_tag="PLS16_02935"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNJ60064.1"
                     /translation="MAIMNFGGVEENVVTREEFPLSKAKEVLKEETIAIIGYGVQGPG
                     QALNLRDNGFNVIIGQRRPSKSWDKAVADGFVEGQTLFEPEQALEKATIIMYLLSDAA
                     QIEMWPTVKKHLTTGKALYFSHGFGITYKERTGIVPPADVDVILTAPKGSGTSLRRLF
                     LQGTGLNSSYAIFQDATGKALDRTIALGIGIGSGYLFETTFQKEVYSDLTGERGVLMG
                     ALAGIMEAQYSVLRENGHSPSEAFNETVEELTESLIKLVSENGMDWMYANCSTTAQRG
                     ALDWKGPFREAVLPVFKELYAKVAAGEEAQRSIDTNSQPDYREKLEAELKELRESELW
                     QAGKAVRSLRPGK"
     gene            complement(10853..11137)
                     /locus_tag="PLS16_02940"
     CDS             complement(10853..11137)
                     /locus_tag="PLS16_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60065.1"
                     /translation="MKGIFYFSGLYLILIDKSVKNYKVEFVSYNQPVMIGRFMLIFSR
                     RRIDVTNFSFQKIDENDGKFTIEFNAEEWDAQNILKQVQKQIDVFEAELV"
     gene            11276..12190
                     /locus_tag="PLS16_02945"
     CDS             11276..12190
                     /locus_tag="PLS16_02945"
                     /inference="COORDINATES: protein motif:HMM:NF017672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006672 - ceramide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ceramidase domain-containing protein"
                     /protein_id="HNJ60066.1"
                     /translation="MATLDFDKLKDTFKLHNHTPKVFIFPFLITFISSSKMFIFYKLN
                     QYIPIWDKFSQADGNATTFCELNHFDQLIVQPSNTWSNIGFIIGGLIILGIAKNDTKY
                     YGKNTINNLLTKYPHFSYLFGGTLLFLGLSSFIYHASLTYFFQKLDITGMYFVLISAI
                     TYNVYKLFPYIRFKNVVYSSHKFMIVCGLALMLFFYLYLWKLPLNIVFPSLVLVFFFM
                     NLYVYAKVKHSIPIKGILITSFVTISISFTFWILDKTSLLCSPSSPFQGHALWHILNA
                     TNIVLLYFYYRSEDFIPEEMNVLQPKSK"
     gene            12276..13193
                     /locus_tag="PLS16_02950"
     CDS             12276..13193
                     /locus_tag="PLS16_02950"
                     /inference="COORDINATES: protein motif:HMM:NF017672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006672 - ceramide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ceramidase domain-containing protein"
                     /protein_id="HNJ60067.1"
                     /translation="MTASNFEKIQKIFKLHNHRPRVFTWPFFGTIIFSGIMFAGYKLN
                     QYITIWDKYKQAGGNATEFCELNRFDQLIVQPSNTWSNIGFIIAALIVLSIAKNDSKY
                     YERSSVNNLLARFPGFSFLFGFSLLYLGLGSFLYHASLTHFFQKLDQTGMYFIVISAI
                     AYNTYKLFPIIRLKNKHISSHKFLIWSAITVMAAFYLYLWKLPINIIFPSLILIFFFL
                     NLYVQTKIEHSKPIKSFLTAAFVTIVFSYAIWILDRTTVLCSPTSPFQGHALWHILNS
                     VCILLVYLYYRSEDYLPEEIQNEDIYETV"
     gene            13180..14388
                     /locus_tag="PLS16_02955"
     CDS             13180..14388
                     /locus_tag="PLS16_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ60068.1"
                     /translation="MKQFKLIVFLFFNFSLFTSCEALANIQSSNKPSNTQQNTQQNNP
                     IGTIRVDPIQYPVSKDRIPTTAVLPGTRINYEQNPYGTRYKNYIFSNYNIKTEAYIQN
                     SVNYSGVSKNLVMDIFTPANDNEVNRPCIIFLYGGGFSMKIDDGLQEICKSMVLRGYV
                     VAAIDYRIGFNGDKVAANCGGNVYNDFLKAELRATQDAIAAIKYIKSNASRLGVNADL
                     IFIGGQSAGAIAALDAVYYDNSETDKNLISSIGGSLDATTANNLRNQSTKVAGVFALS
                     GAISNPKIIENPNNTPTLLVSGTCDELIYSNTGAIYKCDTRAANKALPFPMIYGPEYI
                     YEKLVQKNNPTFTIKVCKGGHAMNNWGYQKVVDWVAGFTYAVMNNTFKSGTAIVYPDK
                     TVCNEDDCSK"
     gene            14396..15400
                     /locus_tag="PLS16_02960"
     CDS             14396..15400
                     /locus_tag="PLS16_02960"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNJ60069.1"
                     /translation="MKAIYIVKFGSPEKAFELRDITISEPKNDEVGIVVEASGINFAD
                     VVARLGDYRDCPPLPAIVGYEVVGKVDKVGSEVKGLKIGDRVLAFTRFGGYAQYVTQK
                     YNAVSKIEEDMAVGEALALATQYSTAFYATNVATNVLPNETVLIHAGAGGVGTALIQL
                     CKLKNAKIYSTAGSDSKLEYLQRQGVDYPINYNKEDFSKVIKEPIDVAFDSLGGDNFR
                     KSYNLLQKGGRIVGYGASSFTDAKNIIDKGIMAAKFGIYHPAQLLIESRSIIGVNMLR
                     IADYKPDTLKFCMDSVINLLKEGKIKPEVSKMYKASDIYAAHHDLENRKTIGKIGIVW
                     "
     gene            complement(15468..16607)
                     /locus_tag="PLS16_02965"
     CDS             complement(15468..16607)
                     /locus_tag="PLS16_02965"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNJ60070.1"
                     /translation="MATKEKEYEVKDHQLSISYLHVWFQLLLRNRISKDKFSMALKIT
                     LFIVLTQPFQWLQYLLFNSKIKQTDINKKQPLFILGHWRSGTTHLHYILSKDEQFSYL
                     TNYQSFLLNVSLIGRTWLKVILSPLLPDKRPQDNVKLTVDSPAEEEQPFSNCSTKAGI
                     HSFFFPQNSDYHEKYHLFKNISPEEKREWQQDYVYLLKKIAFVNGSRQLLLKNPHNTG
                     RVNELLELFPQGKFVFIHRNPYEIYQSMQHHFKKVISTQFLQDYSAEEIHERILYVFE
                     TMLQKYLNEKDLIPKNQLFEIAYAELEQQPMPIIQNLYHQLALPNYENAKSSIVEYLS
                     SVKQYERNNFKNLDKKTIQDINTRWKFAFEIWNYDMISAEGVATI"
     gene            complement(16749..17585)
                     /gene="prmC"
                     /locus_tag="PLS16_02970"
     CDS             complement(16749..17585)
                     /gene="prmC"
                     /locus_tag="PLS16_02970"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNJ60071.1"
                     /translation="MKQVAYQKIILLLLQEIFDERESKNIYKYVLEEYFGQQFIHLDQ
                     LILNDEELEDLNVIFEKTTNHFPIQYIFQKAYFYNFDFFVNEHVLIPRPETEELVQLI
                     LQENDLNDKSILDIGTGSACIPITIKKHRNNWAVSAMDISLDALDVAKKNAKNLQTEI
                     NFIHQDILDINEKSFTKYDIIVSNPPYITPKEKTVMSKSTILYEPHLALFTPDDDALI
                     FYKAIAKFALKNLLPDGKLYFELNEFYAEETKTLLQQYDFKDIRIIKDLSGKNRILSA
                     TI"
     gene            17577..18656
                     /gene="ribD"
                     /locus_tag="PLS16_02975"
     CDS             17577..18656
                     /gene="ribD"
                     /locus_tag="PLS16_02975"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNJ60072.1"
                     /translation="MFQKCIFVQSKYKITKVPTDELYMQRCFELAQLGEGFVAPNPLV
                     GSILVYKDRIIGEGYHQKFGTAHAEVNCINSVHEVDKKFIKESTLYVSLEPCSHFGKT
                     PPCADLIIQQQIKKVIISVLDPNPLVAGNGIKKLENAGINVEFGLLQEDGQSMIEKFI
                     WFHQQQMPFVTLKYAQSADGFIGQNNKTIAISNKMSQRLVHQLRATHQAILIGNNTLK
                     TDNPLLNVRYAKGNNPIKIILCRDGNVDMNLNIFQDISSQIIIINELKETKMDNFHWL
                     KVEDIYNVESILKKLYAYNIQSILVEGGAKILKSFIAQNQWNEIIQIKAKKRLVSGIE
                     APAINFKPYLSFDLAEDKVYRYRNL"
     gene            18735..19286
                     /locus_tag="PLS16_02980"
     CDS             18735..19286
                     /locus_tag="PLS16_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60073.1"
                     /translation="MKANKNILIGILIIAIAVVLRIVQPIDNFAPIVAVTLFAGYLFK
                     SYQKGLILAVLASFIGDVIISYIHQYNLFHDTFVFVYGSYALIAFLGSKMSANKFGWG
                     KLATYGLASSLLFFVITNLGVFLMSNMYSKDVNGLVNCFVAAIPFYKYSFISDALYIP
                     VIFGIHQYVSSLSDSFSGVYIQK"
     gene            19402..20781
                     /gene="glmM"
                     /locus_tag="PLS16_02985"
     CDS             19402..20781
                     /gene="glmM"
                     /locus_tag="PLS16_02985"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNJ60074.1"
                     /translation="MALIKSISGIRGTIGGKSGDNLTPLDIAKFTLAYASIILQKGGN
                     KVVVGRDARISGEMVYHNVVGNLLGLGIDVIDLGLATTPTVEMAVVAESADGGIIITA
                     SHNPRQWNALKLLNKRGEFISESVGAEIIKKSDKAKYDFAEIDKIGKVVQKDYLDYHI
                     QKILALPYVDVEAIAAQDFSVVLDAVNSVGGIAVPKLLNALGVKKIIELYCEPNGKFA
                     HNPEPLPEHLGNLSTEVTFNKADLGIAVDPDVDRLAFIDEKGNPFGEEYTLVACADYV
                     IAQNKGNTVSNLSSTKALRDIAKKYEVEYHASAVGEVNVVEKMKAVDAVIGGEGNGGV
                     ILPELHYGRDALVGIAIFLTHLAKSNKNTSSLRASYPNYVISKNKIQLEEGVDVDKII
                     AGIKDKYKQYPQNTEDGLRIDIDEDWVHLRRSNTEPIIRIYSESSTEQTANAIAHKMQ
                     KDILEVLKN"
     gene            20881..23034
                     /locus_tag="PLS16_02990"
     CDS             20881..23034
                     /locus_tag="PLS16_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNJ60075.1"
                     /translation="MTSFFDHLTHEFTLPLQNPILVFSLILFIILLSPILLRKLNIPG
                     IIGLIIAGVVIGPHGFFILEKNSAIELFSTIGLLYIMFIAGLELDLNEFKANRNKSLL
                     FGFFTFIIPISIGFPVCYYVLGYDFNTSFLTASMFATHTLVAYPIVSKLGVSKNQAVA
                     ITVGGTLLTDTAVLIILAVIMENSQGTLTQEFWIRLGISLAIFSAIIFLVIPRIAKWF
                     FEKLESEKHSHYIFVLSVVFFAAFLAEVAGIEPIIGAFMAGLALNKLIPHSSALMNRI
                     EFIGNSLFIPFFLISVGMLVDIRVILSGPMALIVAGTLTMVAIFSKWLAAFFTRLIFK
                     YSKAQGQLIFGLSSAHAAATLAIILVGYQAGILDENILNGTIILILITCIVASFATEK
                     AAKTIIVESESDVSELVKSAEIQNEHILFPIVSATNIEKVLEFLILIKDKKSVHPISV
                     LSVVSNDAEAEINILKSKNKLEEYVRQASAFEAKVNIITTIDHNPSSGISRIAREIMA
                     DIIMLGWPRQYGFVDKIFGKKLDNLLRSSDKTIFVGHLEKPLALLKRIVIIVPPLAEF
                     ENGFTLWVMKMAKLAQELSIPMVVYSNETSKIAVEKVFSKEKLSAKLYTYLFIDWDDF
                     LVLSKYIQEEDLLVLVSARKGTTSYMRILETIPLKFEKYFAINSRLVIYPQEYDTAII
                     ERFEDISGEPLDRGIEAVQKIGKGIGSIFKGNDQS"
     gene            23145..24095
                     /locus_tag="PLS16_02995"
     CDS             23145..24095
                     /locus_tag="PLS16_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HNJ60076.1"
                     /translation="MSIIPVLDLADYLSDDITRKQKFIQDFGKAYEEIGFAAIENHGI
                     PPELIQRFYQSIQDFFALPTEEKLKYEIVELAGQRGYTSFGREKAKQSEVADLKEFFQ
                     FGQYIEDGEKMPEEDYPDNVEVKEVEDFNKIGKELYQNFEKSGRHLLRAIAEYLELDK
                     NYFDSKIHNGNSILRAIYYPPITQEPKNAIRAEEHEDINLITLLVGASADGLQVLDRN
                     NVWHDAKAEPHQIMVNVGDMLQRLCNNKLRSTTHRVVNPPREKWHTPRFSIPFFLHPR
                     SEMRLDCLENCISAENPIAFEPISAGEYLNERLREIGLKK"
     gene            24174..25025
                     /locus_tag="PLS16_03000"
     CDS             24174..25025
                     /locus_tag="PLS16_03000"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HNJ60077.1"
                     /translation="MYKTTHLHKIPNSFELYSRIATSVIKKPKAFLPNLEVRIDDLSI
                     AQKETNAYNSLLGFGLTNAVPSTFLFVKSFNLKTYLMSSLEMPFPMLGMVHFANKIKQ
                     IEPLNYNQKFSIVCRTGNLIAHSKGQAFEINTSVEVNGKSIWEETMVSLSKGKEGIGE
                     VLEWDIQAVDAKHERRVWNFEDNLGIKYAQASGDFNPIHLHPISAQLFGFKRHIIHGM
                     YTAGKILAQYPKVWNQPHQFAASFKTPIFLPSTVVFRSSEANNKITLDVVDKNETQPH
                     LKGYIQY"
     gene            25027..26289
                     /locus_tag="PLS16_03005"
     CDS             25027..26289
                     /locus_tag="PLS16_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNJ60078.1"
                     /translation="MKDFFKKEAFYTVNTFPEFRYYVIARFLLTMAIAFQSVLIGWEV
                     YLLTDSKLMLGFIGLTEAIPAIGLALYGGHVADKSEKKGLFFKCLIFYILLSATLILL
                     THRDFISYFGKRDVIIGIYAVIFMTGMVRGFSGPAGFALATFLIPKKYYPNGATWTSG
                     AWQLGAIIGSALAGLLYGILGITLTFTLQLFLLVLSTFLISKIQPKPIINKDMTEKIW
                     DSVKVGLRFVFQNKIILSCISLDLFAVLFGGATALLPVFAKDILHVGSFGLGIMRAMP
                     AVGAIIVMIWMAFFPFKKYVGNILLIAVAGFGVFIILFGLSEYFILSLFCLMMSGALD
                     GVSVVIRQTILQLKTPEEMRGRVAAVNSMFIGSSNEIGEFESGLAAKLLGTVPAVIFG
                     GAMTIFVVIVTYFKAPSLRAMHELKADD"
BASE COUNT         9087 a         3567 c         4536 g         9257 t
ORIGIN      
        1 ttactaacgg tgtaaaacaa caatatttag tagcaatagg ctttttgatg ttctttattt
       61 atagtttttg gggctataaa atattaacac cagatactgg cgaacaagac tttttttggc
      121 tattaatagt acgtggtttt ggattgagtt tattatttat tccaattaca actctggcat
      181 taagctccct aaagggaaag gaaataggag agggctctgc atttactgga atgatgaggc
      241 agttaggtgg ttcttttgga atcgctgtaa ttactacatt tgttgccaat atttccacta
      301 aaaatgttgc attgctatca gaacacctaa acgtcaatga tttagatgta caacaaagaa
      361 ttgagatgat gaaacatggt tttatggcta aaggaatgac accagatgtt gcattacaag
      421 cagtatataa agcattagat attacagtac aaaaacaagc tactgtaatt gcatatatgg
      481 atgtttttct ttatttgggc attttgtttt taatatgtat tccatttgtt ttgttaatga
      541 aacaaaagaa aggagctgct cctattgata tgagtgaagc catgcattaa tttgtttagt
      601 tctatttctt ttgaagttat ggagattaac attttaagga ggataacgtt aggctttttg
      661 ttctattcag tatatgaata ttatttttaa atagtatttt attatataaa ttatatattt
      721 ctctaacttc attctgataa actatgaacc atcattagta aactatattg tatgtggata
      781 ttttcctatg atataccctt ataattttgt atatttgtta cctaaattaa gaaagatact
      841 atgtcagaag aattagaagg gcaagactcg agaattgttc caattaacat tgaagaccaa
      901 atgaaatcag catacatcga ctattcgatg tctgttattg tatccagagc tttacccgat
      961 gtaagagatg gtttaaaacc agtacaccgt agagttttat atggtatgca agatttaggt
     1021 ttggcatcta ataaacctca taaaaagtcc gcacgtattg ttggggaagt tttaggtaaa
     1081 taccacccac atggcgatgc ttctgtttat gatacaatgg tgcgtatggc acaggattgg
     1141 agcttgcgtt atccattggt agatggacaa ggtaactttg gttctataga tggagaccca
     1201 ccagcagcaa tgcgttatac tgaagcaaga tttagaaaaa ttgctgagga aattttacaa
     1261 gatattgata aagatacggt tgattttcaa ttgaattttg atgattcatt gaaagaacca
     1321 acagtaatgc caactagaat accacaatta ttagtgaatg gtgcttcagg gattgcagta
     1381 ggtatggcaa caaatatgtt gccacacaac ctttctgaag ttgtagatgg gattgtggct
     1441 tatattgata acaatgctat cgaaattgag gaattaatta agtatgttaa agctcccgat
     1501 tttccaacag gcggtataat ttatggctat gaaggaatta aagaagcctt tttaacagga
     1561 agaggaaaag ttcgtcttag aggaagagca actatcgaag aaaacaaaga tggccgaaca
     1621 caaattataa tctctgaaat tccttaccaa gtacagccaa agatgataac aacccgtgcg
     1681 gcagaattgg tgaatgataa aaaaattgat ggcattgctg aaattagaga tgaatcagat
     1741 aaagatggtt tgcgtatcgt ttttgactta aaaagagatg caattcctaa tgttgtatta
     1801 aataatttat acaattttac accgttacag tcaagctatg gtatcaataa tatcgcatta
     1861 gtagcaggaa gacctcaggt tttgaatctg aaagatttaa tcagacattt tgtggatttc
     1921 agaatggagg tggttattcg aagaacacaa tttgaactca atgaagctga gaagcgtgcc
     1981 catatcttgg aaggttttat gattgtgttg gaaaatgcag ctattactga tgaggttatc
     2041 gcattgatta gaaaatctaa aaatccagaa gcagctaaag atgcattaat ggaaagattt
     2101 ggattaagtg aaattcaatg taaagcaatt ttggcactcc aattatcgag attaacagga
     2161 atggaaatcg aaaagattaa agaggagtac agacaaatta tggaattaat tggtaagttg
     2221 aaagatattt tagataaaga ggcgttgaga tttgaaatta tcaaagaaga gatattagaa
     2281 ataaagcgaa aatacggaga cgaaagacgc tctgaaattg tttattatgg tgatgatatt
     2341 tcttatgagg atatgattcc tgaagaagaa atggtggtta ctatctcaca tttgggctat
     2401 gtaaaacgta catctttaga cgaatacaaa aagcaaaata gaggaggaaa aggctcgttt
     2461 ggtggcaaaa caagagatga agattttatt gaacatattt ttgttgcatc aacacacaat
     2521 tatttattgt tatttactga attaggaaaa tgtttttggt taaaagtata tgaagttcct
     2581 gaaggcagta aaacagccaa aggaagacca attcagaatg ttatcaatat tccgcaagaa
     2641 gataaaatca aagcttttat taatgtcgca gatttgaaaa atgaagagtt gttagatagt
     2701 cattttataa taatggctac caaaaaaggt actgtgaaga aaacttcatt aagagaatat
     2761 tctcgtccaa gacaaaatgg tgtaaatgca ataacagttc gagaaggcga tgagttagta
     2821 tctgcagata ttactaacgg aaaatgtgag ataattttag gttcaaaagc tggaaaagca
     2881 gttcgttttg aagaagctga tgcacgtgca attggtagaa cagcttctgg tgttcgtggt
     2941 attacattaa aagatgaggc agacgaatta atcggtatgg tttgtattga tacatcttca
     3001 gagcttacac ctgatattat ggttgtttct gaaaatggct atggcaaacg ttcattatta
     3061 gaagattatc gtaaaactgc tcgtggagga aaaggcgtta ctacattaaa tattacggat
     3121 aagacaggtg aattaattgc aatcaatgcc gtaactgacg ataatgactt gatgattatt
     3181 aataaatcag gattaacgat tagaattggt ttagatacaa ttcgtactat gggaagaaat
     3241 acgcagggtg tgagcttaat taaattgaaa gaaaatgatg caattgctgc tgtaaccaag
     3301 atagctcatg aggaggaaga agaagaaact gctgaaaatg gtacggatat tgataataat
     3361 tccatagaaa ataattctaa cgaacttaac gaaaatagta atatcgaaaa taaagaagat
     3421 taatatgaag aaaataactt tagttttaat tggtgtctta ttagtaaatg taatctttgc
     3481 tcagaaaggt aaggtaaata gtgcatcgta tgcactttct gaaggcaaag caaatgaggc
     3541 aaaacaattt attgatgaag cattgacaga tgctgaaaca caacaaaatg caaaagcttg
     3601 gcaaataaaa ggtgatgttt acaaaaatat ctatgaagga aaagtgtttt ttgctcaaac
     3661 accaaatgcc ctagaagatg caaaagctgc ttatttaaaa gcttatgaat tggaagttaa
     3721 tccaaagaaa aaaggtgtag ttgcaccaag tttagaacac ttacaaggtt atttctttaa
     3781 cgaagggcta tctcgttttc aagatgaaaa atggactgaa gcgtatatga agtttagtga
     3841 agcagtaaat attaatgaat ttttgttggc aaacaactta gctaaaacaa tagattcagg
     3901 tgcatatttt gcaactgctg ttgctgcaaa taatgcagga ttattggaca aagcaaaacc
     3961 tcttttagaa aaacttgtgg ctttaaaata taataacaaa gcgatttatg aaagtttagc
     4021 agatatttat gcaaaagaag gaaatccaaa ttttatgaat attgtaaatg aaggattaaa
     4081 gaagtatcca gatagcaaaa cattgcaagt ttcaagattg aactattata ttgaaaatgg
     4141 aaaaacagat gaggcgattg cagaaataca aaatgcattg aatgctgacc caaaaaatta
     4201 tctattatta tttaatatgg ctgttttaca agagcaatta ggaaattacg atgatgctaa
     4261 aatatattat gagaaagcta ttgctgcaaa accagatttt atggatgcat attttaacgc
     4321 tggagcatta tattacaaca aagcaattga aattaataaa gttttagtta aagatgatgc
     4381 agcaagtata gatagtactt tagctgaatt tgcttcaaaa gatgtgattt caattgatga
     4441 tattcgtaat gcactaaaag acccaaatat gggtgcttta ttaaatagtt ttcaaaatta
     4501 ttcaggggac aaagctacat taaaagataa agttagtaag ataaaagaaa atattaaata
     4561 cgatttattg attgcaaaac gtaatgcatt attcaataaa actttaccat tttttgaaaa
     4621 agcatacagc attcaaaaag aagatcagct gaaaagtgca ttgaaagaaa tttatgctcg
     4681 tatgaatatg ttcgataaag tcaaagcatt agaccaataa ttatcaagta aagctttaat
     4741 aattataaaa agaggctgtc tgaaaaattt tggacagcct ctttttattt gtgtagttat
     4801 ttaggtagta tctccaactg tgtaagctgt aagttttata aattatctat tgctttgagt
     4861 aattggtcaa cagcttcttt atttacgtta ccacccatta tgcctatacg ccataatttt
     4921 ccgaacattg aacctaatcc agctcctact tcgactttaa attcatttct catttttgtt
     4981 ctattacttg catcatcaaa accttcaggt aataaaacag tattcaattg aggtaatttt
     5041 gcatcgcctt caacaaagaa tttccaacct cttgaggtca tttcttggaa gaaataggca
     5101 ctaatttcaa tatgttgatt ccaaattttt tctaaaccaa tagatttaat atgtcttaat
     5161 gccgccaata ttgcataata ttgttgtgat ggtgcagtat gatgatatgg acgtgccgtt
     5221 tgtgttgccc aatatttttc atatactgtg atgtcttgaa accacgatgg aactggattt
     5281 tttctattga gtactttatc aaacgcagcc tgactaaaag ttactggaga catacctggt
     5341 acactaccaa ttcctttttg actgcatgaa tatactgcat ctaatcccca ctcatctact
     5401 ttcaatggaa tagcacctaa tgctgtaact gcatcaacaa taattaaagc accgtgtttt
     5461 ttagctaatt tagcaatggc ttctacatca ttttttacac ctgtggaagt ttctgcatgt
     5521 actactgaaa ttaatttcac attaatattt gtattaaaag cattttctac atcatctaat
     5581 gaaattggtt ttccccactc agccgtaaca cttataattt cagctccata tcgtttagca
     5641 ttttcatgca ttctatcacc aaaataacca ttatggcaac agataatttt atcgcctgat
     5701 tcaactaaat tgacaaaaca agtttccatt cctaaagagc ctgggccaga aactacataa
     5761 gtgtgagggc tttgagttac aaataaatat tgaaggtgtt ctttaatttc atccatgatt
     5821 tttaaaaaat cagggtctaa atgacttaat acaggctctg acattgcttg taatacgcca
     5881 ggcattgaat tagaagggcc tggtcccatt agtaaaattt cagcagtttg tactggattg
     5941 atttgtgaca tattattttt tttacctaaa tattgaattg tggcaaatat agttaaatta
     6001 aaagactttt tgagcttagt ttatataaat gattttgtat gtattgatga aatggaaata
     6061 gaattgttgc tcaataacaa ttgatggact tatgctttat atattggata gtatttctga
     6121 aaaatgaaaa ggtaatataa ttattcagat acgctgattt gcttaattac tatgtttctt
     6181 catagttgat tttttcatta acatttattc aagctgtgga ataatattag ttttaggcat
     6241 gaccatttta ttgcagtctt cgtatcttta ccattaacta aaataagttg agcgtagatg
     6301 agcaaaccca aagaagtgaa agaaacacct ttaatgcaac aatacaacaa gataaagcaa
     6361 acttatcctg atgctatttt gttgtttaga gtgggtgatt tctatgaaac atttggaagc
     6421 gatgctgtca aagctgcaga aattttggga attgtattga ccaaacgtgc caatggctca
     6481 caaacagatt tggagctagc agggtttcct catcattcag tagatactta tttacctaaa
     6541 ttagtaaaag caggttatcg tgttgcaatt tgcgagcaat tggaagaccc caaaatgaca
     6601 aaaacaattg taaaacgtgg tgttaccgaa ttaattacgc caggtgttac agtaaatgat
     6661 aaaattttag accataaagc caataatttt ttagcctcag tcttttatga aaatgatgcg
     6721 atgggcattg cttttttaga tatttctact ggagaatttt ttgtagctca gggtaattta
     6781 gattatttgg ataaattatt tcaaagttat acaccttcgg aagttgtctt tccaaagttc
     6841 caacagaaaa agtttaatag tacttatgct aataagtttt atacttatgc cattgatgat
     6901 tgggtatatc aagatgatta tttggaaaaa aaattgttga accattttca agtgcaaact
     6961 ttaaaaggtt ttggtttaga taatttggct ttaggcatca aagctgctgg tgcttgttta
     7021 caatatttga atgaagcaga acaaaaatca ctcgaccata ttacacaaat tcaacgtatt
     7081 gaaaataatc attttgtatg gttagataga tttactgctc gaaacttgga attagtacat
     7141 tccatgcacg aaaaaggggt tacacttttg aatattattg ataaaactgt agctccaatg
     7201 ggtagccgat tgatgaggcg ttgggtagcc atgcctttaa aaaataaagt aactatagat
     7261 gaacgtttac aagcagtaga atatctaatt aaagaacaag ataattcaaa taaattatca
     7321 caaagtataa aaacaattgg ggatttagaa cgaattattt ctaaggtttc tttattaaaa
     7381 atatcgccta gagaactaaa tcaattaaaa aaagcattgt cttcaattgc agatattcaa
     7441 attttatgtc agcaatcgga aaatattaca ctaaagaaaa tcggcgaaca aattaattta
     7501 tgttcttcag taaaagataa aattgaagta acaattacag acgatgcacc tgtggctatt
     7561 caaaaaggaa atgctgtaaa aataggtatt tcagaggagt tagatacctt gcgtgctatt
     7621 tctttatctg gaaaagatta tttaaccaaa cttcaaaata cagaaattga aaagacagga
     7681 attagttctt tgaaaattgg atttaataat gtttttggtt attttttgga agttagaaat
     7741 acccacaaag aaaaagtgcc tgagacttgg attcgcaaac aaacattagt aagtgctgaa
     7801 agatatatca cacccgaatt aaaagaatat gaagagaaaa ttttaggtgc ggaagagaaa
     7861 atccaaaaaa ttgaagagca aatttttact gaacttatac attacatcat tgactatatc
     7921 aagccaattc aattaaatgc tcaactcgtt gctcaattgg attgcttatt gtcttttgca
     7981 actgttgcat tgaaaaataa ttatagaaaa cctataatta ctgaagataa tatcctgaaa
     8041 ataaaagaag gtcgtcatcc agttattgaa caacaattgc ctttaggtga acaatatatt
     8101 cctaatgatg tttttttgga taatgaatca caacaaatta tcattataac gggaccaaat
     8161 atgagtggta aatctgctat tttaaggcaa accgcactca ttgttttgtt ggcacaaatg
     8221 ggttgttttg ttccagctca atcggctgaa attggtatca ttgataaatt atttactcgt
     8281 gttggagcaa gtgataattt gtctttgggt gaatctactt ttatggtaga aatgatagaa
     8341 actgcaagta ttattaataa tatttcagaa aagagtttgg tacttttaga tgagattgga
     8401 cgaggtactt caacttatga cggtatttct attgcttgga gtttggtgga atatctacac
     8461 aataataaaa cagctcgtcc caaaacaatg tttgctacac attaccacga attaaatgaa
     8521 ttggcagata aatttgaaag aattagaaat tataatgtcg ctgtccaaga aactggtaat
     8581 aaagtattgt ttctacgaaa attggttgaa ggtgggagtg gacacagttt tggaatacat
     8641 gttgcaaaaa tggctggttt gcccaatgcc atcatcaata gagcaaatga gattttagga
     8701 gaattggaaa gcaatagaag tatttcttcc gatatctcaa aaactcttaa aaagctacct
     8761 gaaaatcaaa attttcaatt gaatatgttc tcgatgcaag acccacgatt attgaaactt
     8821 aaagaagtta ttgaacaagt agatatcaat acaatgacac cagttgaagc gcttttgaaa
     8881 cttagtgagt tacgcaaatt actttaatag tttggtgata tcttcaaatt taaccgcata
     8941 aatatgtgca tgactacaag caatatagcc agctaaattt gataaataag caattgcttg
     9001 cattgaatta ttggataagt tacttaaaca tagcattgca attacactat ctccagcacc
     9061 acaaacatca actttttcta tagattgatg aattgcttga cggtgaattt tattttgttc
     9121 attcatagaa atgctaccat cttttcctaa tgtaataaat agattttcta cttgaagata
     9181 gttttttaga tacaataatt gttcttcaat ggataattca tctattttta ataaatgcgt
     9241 cattgcatca aattctattg cattaggttt aaaaaaatct acctttttat ataattccca
     9301 attnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnattgatg atattttcat
     9421 caaggtttca atattcttga actctggttc ataagtctgc ccaacaattc caactaagta
     9481 tggagtagca cccattgttt taatattact ggcaacattt gctgctccac ctaaacagta
     9541 actacatttt tctactttta aaactggaac ttcagcatat tcaggcgaca tttttgcata
     9601 ttcagcataa acaaatttat ccaaaattat atctccaata actaagatac gtttgtcttc
     9661 aaaatttaga tttatttcta gttccatact ttcaaaaata attaaattat agcaataaaa
     9721 aaccccaaac aaaagtttgg ggcaaatatc ttttttagga aaaaattatt ttccaggacg
     9781 taagcttcgt acagcttttc ctgcttgcca taattcactt tctcttaatt cttttaactc
     9841 tgcttctaat ttttctctgt agtcaggttg agaatttgta tcgatacttc tttgagcttc
     9901 ttcacctgca gcaacttttg catataactc tttaaatact ggcaaaactg cttcacggaa
     9961 aggacctttc caatctaacg caccacgttg agcagtagta gaacagtttg catacatcca
    10021 atccatacca ttttcagaaa ctaatttaat taaagactct gttaattctt caacagtttc
    10081 attgaaagcc tcagatggtg agtgtccatt ttctcttaat acactgtatt gagcttccat
    10141 gatacctgct aaagcaccca tcaaaacacc acgctcgcca gttaagtcag aatatacttc
    10201 tttttggaaa gtagtttcaa ataaatatcc agaaccaata ccaattccta aggcaattgt
    10261 tctatctaaa gcttttccag ttgcatcttg gaaaattgca taagatgaat ttaaacctgt
    10321 tccttgtaag aataatcttc ttaatgaagt tccagaacct tttggtgcag tcaaaataac
    10381 atctacatca gctggtggaa caataccagt tctttctttg tatgtaattc caaaaccatg
    10441 agagaaatat aatgctttac cagtggttaa atgttttttt actgttggcc acatttcaat
    10501 ttgtgcagca tcagacaata aatacataat aattgttgct ttttctaaag cttgttctgg
    10561 ttcaaacaaa gtttgtcctt ctacaaaacc atcagcgact gctttgtccc atgattttga
    10621 aggtcttctt tgaccaataa tcacattaaa tccgttatct ctaagattta atgcttgtcc
    10681 aggaccttgt acaccataac caataatagc gatggtctct tcttttaata cttcttttgc
    10741 ttttgaaagt ggaaattctt cacgtgtaac tacgttttct tctactccac caaaattcat
    10801 tattgccatg atttcttaat ttgtaatttt atttattaat caatttaatt tattatacta
    10861 attcagcttc aaaaacatca atttgctttt gtacttgttt taaaatattt tgtgcgtccc
    10921 attcttccgc attaaattca atcgtaaatt taccatcatt ttcatctatt ttttggaaag
    10981 aaaaattagt aacatctatt cttcttcttg aaaagataag cataaaacga ccaatcatta
    11041 caggttgatt ataggataca aattctactt tataattctt cacgctcttg tctattaaaa
    11101 ttaaatatag tccgctaaaa taaaaaattc ctttcatttt atcaaataag gtgtattaac
    11161 ataatataaa ttgctaaaat ggtagatata ccaacctcaa ttattttaaa ttatttgcat
    11221 aataaaaaat aagtacctat ttttagtgat ataattaaaa taaaataaat aaattatggc
    11281 aactctcgac tttgacaaat taaaagatac ttttaaatta cataaccata cgccaaaagt
    11341 tttcatcttt ccttttttga ttacttttat ttcgtcatct aaaatgttta ttttctataa
    11401 actcaatcaa tatattccaa tttgggataa attcagtcaa gctgatggaa atgcaactac
    11461 attttgtgaa ctaaatcatt ttgatcaact gatagtgcag ccatcaaata cttggtcaaa
    11521 tataggtttt attattggag gattgattat tttaggtatt gctaaaaatg atactaaata
    11581 ctatggcaaa aatactatca ataatctctt aactaaatat cctcatttta gttacttatt
    11641 tggcggaact ttattgtttt tgggactttc tagttttatc tatcatgcaa gtttgactta
    11701 tttttttcaa aaattagata taacaggaat gtattttgtt ttgataagtg caataaccta
    11761 taatgtctat aaattatttc cttatattcg atttaaaaat gttgtttata gctctcataa
    11821 atttatgata gtttgtggat tggctttaat gctgtttttt tacttatatc tatggaaact
    11881 tcctttaaac atagtatttc cgagtttagt attagtattt ttctttatga atttatatgt
    11941 ttatgctaaa gtgaaacata gtattccaat taaaggcata ttgattactt catttgtaac
    12001 tatttcaatt tcattcacat tttggatatt agataaaact agtttgttat gttcgccttc
    12061 aagcccattt caaggtcatg cactttggca tattttgaat gcaactaata ttgttttact
    12121 ttatttttat tatagaagcg aagattttat tccagaagaa atgaacgttt tacaacccaa
    12181 atcaaaataa tatttttaga ataagtttgg tattattttt gccaaaatat ctattggaaa
    12241 tattatattc gcataaataa tatctaagta taataatgac agcatcaaat ttcgaaaaaa
    12301 ttcaaaaaat atttaaacta cacaatcaca gacctagagt ttttacttgg ccattttttg
    12361 gaacaattat cttttctgga attatgtttg caggttataa attaaatcaa tatataacca
    12421 tttgggataa gtacaaacaa gcaggaggta atgctacaga gttttgtgaa ttgaatcgtt
    12481 ttgaccaatt aattgtacaa ccttctaata cttggtctaa tataggattt attattgcgg
    12541 cattaatagt tttaagtatt gctaaaaatg atagtaaata ctatgaaaga agttctgtga
    12601 ataatttatt agctcgattt cctggtttta gtttcttatt tggattttca ctattgtatt
    12661 taggattggg tagtttttta tatcatgcta gtcttacaca cttttttcag aaattagacc
    12721 aaacaggcat gtattttatc gttattagtg cgattgccta taatacttat aaattatttc
    12781 cgattataag attgaaaaat aagcatatta gttcccataa atttttaatt tggagtgcaa
    12841 ttacagtaat ggctgctttt tatctttatc tatggaaact tcctatcaat attatattcc
    12901 caagtttaat tttaatattt ttctttttga atttatatgt acaaacaaag attgagcata
    12961 gtaaaccaat taaatccttt cttacagcgg catttgtaac cattgttttt tcttatgcta
    13021 tatggatttt ggatagaact acggtacttt gttcaccgac tagccctttt caaggtcatg
    13081 cactttggca tattttaaat tcagtatgta ttttattagt ttatttatat tatagaagcg
    13141 aagattattt acccgaagaa atccaaaatg aagacattta tgaaacagtt taagttaatt
    13201 gtatttttat ttttcaattt tagtttattt acatcttgtg aggcattagc caatatacaa
    13261 agtagtaata aaccatcaaa tacacagcaa aacacgcaac aaaataaccc aattggaact
    13321 attagagttg atccaattca atatccagta agtaaagaca gaattccaac tactgctgta
    13381 cttccaggaa ctcgcataaa ttatgagcaa aatccttatg gtactagata taaaaactat
    13441 atatttagta attacaacat aaaaacagaa gcctacattc aaaattcagt gaattattca
    13501 ggtgtttcta aaaatcttgt aatggatatt tttacacctg ctaatgataa tgaagtaaac
    13561 agaccttgca ttattttttt atatggcggt gggttttcaa tgaaaataga tgatggttta
    13621 caagaaattt gtaagagtat ggtattacga ggatatgtcg ttgctgcaat agattataga
    13681 atcggattta atggcgataa agtagcagct aattgtggag gaaatgtata taatgatttt
    13741 ttaaaagcag aattaagagc aacacaagat gcgattgctg caattaaata tataaaaagt
    13801 aatgcttcca gattgggagt taatgctgat ttaattttta ttggtggaca aagtgctgga
    13861 gcaattgcag ctttagatgc agtttattat gataattcag aaacagataa aaacttaatt
    13921 tcgtctattg gaggttcttt agatgcaaca acagcaaata atttaagaaa tcaatccaca
    13981 aaagttgctg gtgtttttgc attgtcaggt gcaatttcta accctaaaat aatagaaaat
    14041 cctaataata cgcctacctt attagtgagt gggacatgcg acgaattaat atattccaat
    14101 actggtgcga tttataaatg tgatactaga gctgcaaata aagcactacc atttccaatg
    14161 atttatggtc cagaatatat ttatgaaaaa ttagtacaaa aaaataatcc aactttcaca
    14221 ataaaagtgt gcaaaggtgg acatgccatg aataattggg gctatcaaaa agttgtagat
    14281 tgggttgctg gatttactta tgctgtaatg aataatacat tcaaaagtgg aactgctatt
    14341 gtttatccag ataaaactgt atgtaatgaa gatgactgtt caaaataaat aaaatatgaa
    14401 agcaatttat atcgtcaaat ttggctctcc tgaaaaggct tttgaactta gagatattac
    14461 gatttctgaa ccaaaaaatg atgaagtagg aattgtagtt gaagcttcag gaataaattt
    14521 tgctgatgta gttgctcgat taggagatta tagagattgt ccacctttgc cagcaattgt
    14581 tggttatgaa gtagttggta aagttgacaa agtaggttcg gaagtaaaag gtttaaaaat
    14641 tggagataga gttttggcat ttacaagatt tggaggttat gctcaatatg taacccaaaa
    14701 atataatgca gtttcaaaaa tagaggagga tatggcagtt ggcgaagctt tagctttagc
    14761 aacacaatat tcaacggctt tttatgctac aaatgtggct acaaatgtgc taccaaatga
    14821 aactgtccta attcatgctg gagctggtgg tgtgggaact gcactaattc aattgtgcaa
    14881 acttaaaaat gccaaaatat attcaacggc aggtagtgat tcaaaattag agtacttaca
    14941 aagacaaggt gttgattatc caataaacta taataaagaa gatttttcaa aagtcatcaa
    15001 agagcctata gatgttgctt ttgatagttt aggtggtgat aattttagaa aatcttataa
    15061 tcttttacaa aaaggtggtc gaattgtagg ttatggagct tcaagtttta cagatgcaaa
    15121 aaatatcatc gacaaaggca taatggcagc aaaatttggt atttatcatc cagcacaatt
    15181 attgatagaa tcaagaagta ttattggtgt aaatatgtta agaattgctg attacaaacc
    15241 agatacttta aagttttgta tggatagtgt aataaattta ttgaaagaag gaaaaataaa
    15301 acctgaggtt agtaaaatgt acaaagcttc agatatttat gcagcacacc atgatttgga
    15361 aaatagaaaa accataggaa aaattggtat tgtttggtaa gtatcttttt agccaagctt
    15421 cccaaagtat aaaattgtaa cctaaatttc gactataact agatatttta gatagtcgca
    15481 actccttcgg ctgaaatcat atcataattc caaatttcaa acgcaaattt ccatcgagta
    15541 ttgatatctt gaatggtttt tttgtctaaa tttttgaaat tatttctttc gtattgtttt
    15601 acagagctta aatattcaac aattgaactt tttgcatttt cataattagg caaagctaat
    15661 tgatggtata aattttggat aataggcata ggttgttgtt ccaattcagc ataagcaatc
    15721 tcaaacaatt ggttttttgg aattaaatct ttctcattta agtatttttg taacattgtt
    15781 tcaaaaacat ataaaatacg ctcgtgtatt tcttcagctg aataatcttg taaaaattgt
    15841 gtgcttatca cttttttaaa gtggtgttgc atagattgat aaatttcgta tggatttcta
    15901 tgaataaaaa cgaatttacc ttgtgggaat aattctaata gttcatttac acgcccagta
    15961 ttgtgtgggt tttttaacaa aagttgtctg cttccattta caaaagctat ttttttcaat
    16021 aaatagacat aatcttgttg ccattctctt ttttcttcag gagagatatt tttaaataaa
    16081 tgatattttt cgtggtaatc cgagttttga gggaaaaaga aagaatgaat acctgctttt
    16141 gttgaacagt ttgaaaacgg ttgttcttct tcagctggcg aatctacggt tagttttaca
    16201 ttgtcttgtg gtcttttgtc aggcaataat gggcttaaaa ttacttttaa ccaagttctt
    16261 ccaattaaac ttacattcaa taaaaaggat tgataattgg tcagataact aaattgttca
    16321 tcttttgata aaatataatg caagtgtgtt gtaccacttc tccaatgtcc taaaataaat
    16381 aaaggttgct ttttgtttat atcagtttgc tttatttttg aattaaataa aagatattgt
    16441 aaccattgaa aaggttgtgt cagcacgata aataaagtga ttttcaatgc cattgaaaat
    16501 ttatctttgc taattctatt tcgtagtaat aattgaaacc aaacgtgtag ataagaaatt
    16561 gaaagttgat ggtcttttac ttcgtattct ttttcttttg ttgccattgg aaattttttg
    16621 caaaattaca caatctaaat tgacttcata tcattgtata taaggatgaa aatataccta
    16681 taaatcatta gttgaaaaat gttatttttg atatattttt tatgttttat aaaataaatt
    16741 attaataatt atatagttgc agataaaatt ctgtttttgc cagacaaatc tttaataatt
    16801 ctaatatctt taaaatcata ttgttgcagt agcgtcttag tttcttcagc ataaaattca
    16861 tttaattcaa aatataattt tccatctgga agcagatttt taagagcaaa ttttgcaata
    16921 gctttgtaaa aaattaaagc atcgtcatca ggtgtaaata aagccaaatg tggttcatat
    16981 aaaattgtac ttttgctcat tacagttttt tcttttggcg tgatataggg tggatttgaa
    17041 acaattatat catattttgt aaatgatttc tcattaatat ctaatatatc ttgatgaata
    17101 aaattaattt ctgtttgcag atttttagca ttttttttag caacatctaa tgcatctaaa
    17161 ctaatatcca tagcacttac agcccaatta tttcgatgct tttttattgt aatcggaata
    17221 caagcacttc cagttccaat atctaaaata gatttatcgt ttaagtcatt ttcttgtaat
    17281 ataagttgta ccaattcctc agtttctggt cttggaatta aaacatgttc attcacaaaa
    17341 aaatcaaaat tatagaaata tgccttttga aaaatatatt gaatagggaa gtggttagtt
    17401 gttttttcaa aaattacatt caaatcttct aattcttcat cattaagaat tagttggtca
    17461 agatggataa attgttgccc aaaatattct tccaatacat acttataaat attttttgac
    17521 tctctttcat caaaaatttc ttgaagtagt aataatataa ttttttggta ggcaacttgt
    17581 ttcaaaagtg tatttttgta caaagtaagt ataaaataac gaaagtgcca actgatgaat
    17641 tatatatgca acgatgtttt gaacttgccc aattgggcga gggatttgtc gctccaaatc
    17701 ctttggtagg ttcgattttg gtttataaag atagaattat tggcgaaggt tatcatcaaa
    17761 aatttgggac ggctcatgca gaggtaaatt gtatcaattc agtgcatgaa gttgataaaa
    17821 aatttattaa agaaagtact ttgtatgtga gtttagaacc ctgttctcat tttgggaaaa
    17881 caccaccttg tgccgatttg attatacaac aacaaattaa aaaagtaatt atttcagttt
    17941 tagatcctaa tcctttggtt gctggaaatg gtattaagaa acttgaaaat gcaggaataa
    18001 acgtggaatt tggtttgtta caagaagatg gtcaaagtat gattgaaaaa tttatttggt
    18061 ttcatcaaca acaaatgcct tttgttacgt tgaagtatgc acaaagtgcc gatggtttta
    18121 ttggtcaaaa taataaaaca atagcaataa gtaataaaat gagccagcga ttggtacatc
    18181 agctacgagc aacacatcaa gctattttaa ttggtaataa tactttgaaa acagacaatc
    18241 ctttattaaa cgttcgttat gcaaaaggaa ataatcctat caaaataatt ttatgtagag
    18301 atggaaatgt agatatgaat ttgaatattt ttcaagatat ttctagtcaa attataatta
    18361 tcaatgagtt gaaagaaact aaaatggata attttcattg gttaaaagtg gaagatatct
    18421 ataatgttga atcaattttg aaaaaattgt atgcatataa tattcaatct attttagttg
    18481 aaggtggagc aaaaattttg aaatctttta tcgctcaaaa tcaatggaat gaaattattc
    18541 agattaaagc aaaaaaaaga ttagtaagtg gaatagaagc tccagctata aattttaaac
    18601 cttatttgtc gtttgattta gctgaagata aagtttatag gtatagaaat ttataaacag
    18661 ttgctgatgt actcttagtt ttactttttg aaataaataa aataagctgt atatttgtag
    18721 tctaaaatta ccttatgaaa gcgaataaaa atattttaat tggaatttta attatagcca
    18781 ttgcagttgt actgagaata gttcaaccta tagataattt tgcacctata gtagcagtta
    18841 cattatttgc aggttatttg tttaaaagtt accaaaaagg tttaatattg gctgttttag
    18901 cctcatttat tggagatgtt ataatttctt atatccatca atataattta tttcatgata
    18961 catttgtttt tgtgtatggt tcttatgctc taattgcgtt tttaggtagc aaaatgagtg
    19021 caaataaatt tggttgggga aaattagcaa cttatggttt agcttcatct ttattgtttt
    19081 ttgtaatcac gaatttaggt gtgtttttaa tgtctaatat gtattcaaaa gatgtaaatg
    19141 gattagtaaa ttgtttcgtt gcggcaattc ctttctataa atattctttt ataagtgatg
    19201 cactttacat tccagtaata tttggtattc atcagtatgt tagctcactt tctgattcat
    19261 tttcaggtgt ttatattcaa aagtaatatt gcattgtaaa cattgttttt tctaaacatt
    19321 atcttttgga aagataattt tatagtcatt gaaaaaagca atcataattg ctatattcgt
    19381 tcaacataaa attttaatat tttggcttta ataaaatcaa tctctggtat tcgtggtacg
    19441 ataggtggga aaagtggcga taatttaacg cctttagata ttgcaaaatt tactttagcg
    19501 tatgcaagta ttattttaca gaaaggtgga aataaagtag ttgtaggtcg tgatgcaaga
    19561 atttctggcg aaatggttta tcataatgtg gtaggaaatt tattgggatt aggaatagat
    19621 gtgatagact taggtttagc gacaacacca actgttgaaa tggctgtggt tgcagaaagt
    19681 gctgatggtg gtattattat tacggctagt cataatccaa gacaatggaa tgctttaaaa
    19741 ttattaaata aaagaggtga atttatttca gaaagtgtag gtgcggaaat tattaaaaaa
    19801 tcagataaag ctaaatacga ttttgctgag attgataaaa ttggtaaggt tgtacaaaag
    19861 gactatttag attatcatat tcaaaagata ttagccttac cttatgttga tgttgaagcg
    19921 atagcagcac aggatttttc tgttgtttta gatgccgtaa acagtgtggg tggaattgca
    19981 gttccaaagt tgttaaatgc tttgggtgtt aaaaaaatta ttgaattata ttgtgaacca
    20041 aatggaaaat ttgcacataa cccagaacca cttcctgaac atttgggaaa tctttcaaca
    20101 gaggttactt tcaataaagc agatttaggt attgcagtag atccagatgt cgatagatta
    20161 gcatttattg acgaaaaagg aaatcctttt ggtgaagaat atactttagt agcttgtgct
    20221 gattatgtta ttgctcaaaa taaaggtaat actgtttcaa atctatcatc tacaaaagct
    20281 ttgcgagata ttgcaaagaa atatgaggta gaataccacg caagtgcagt aggagaagtg
    20341 aatgttgtgg agaaaatgaa agcagttgat gcagttattg gtggagaagg aaatggcggt
    20401 gttatcttgc cagaattaca ttatggcaga gatgctttgg ttgggattgc aatatttcta
    20461 acgcatttag caaagtctaa taagaatact tcttctctta gagcaagtta cccaaattat
    20521 gtaatttcta agaataaaat ccaattagaa gagggcgttg atgtcgataa aattattgct
    20581 ggtattaaag ataaatacaa acaatatcct caaaatacag aagatggctt aaggattgat
    20641 attgatgaag attgggtaca tttacgtcgt tctaatacag aacctatcat tagaatctac
    20701 tctgaaagta gtacagaaca aaccgcaaat gctattgctc ataaaatgca aaaagacatt
    20761 ttagaagttt tgaaaaatta atcttttttt agaaagatta gatttgttat tggaaatatc
    20821 ttttgatttt tgcagaaaaa tatgtatttt tagattgatt gtttacttta acccaatcct
    20881 atgacttctt ttttcgacca tcttacacac gaatttactt tgcctttaca aaatccaatt
    20941 ttggtttttt cattgatatt atttatcatc ttattgtcgc caattttact aagaaaatta
    21001 aatattcctg gtattattgg attaattatt gctggtgtcg ttattggacc acatggtttt
    21061 tttattcttg aaaaaaattc agcaattgaa cttttttcga ctattggttt attatatatt
    21121 atgtttattg caggtttgga attggaccta aatgaattta aagccaatcg aaataaaagc
    21181 cttttgtttg gattttttac gtttataatt cctatcagta ttggttttcc tgtttgctat
    21241 tatgttttag gatatgattt taataccagc tttttaactg caagtatgtt tgctacgcac
    21301 actttggtag cttatccaat tgtaagcaaa ttaggggttt ctaaaaatca agctgttgct
    21361 attacggttg ggggaacatt attaacagat accgccgtct tgattatttt ggctgtaatt
    21421 atggaaaata gccaaggcac tttgacacag gaattttgga tacgtttagg tatttcttta
    21481 gctatttttt cagcgattat ctttttggtt attcctagga ttgcaaagtg gttttttgaa
    21541 aaattagaaa gcgaaaaaca ttcccattat atctttgttt tatccgtagt tttttttgct
    21601 gcatttcttg ctgaagtggc aggtatagaa cctatcattg gagcatttat ggctggattg
    21661 gctttaaata aattaattcc acattcttca gctttaatga ataggataga gtttatcgga
    21721 aattcacttt ttattccatt tttcctaatc agtgttggta tgctggttga tattcgagta
    21781 atattaagtg ggcctatggc tttaatagta gcaggaactt taacgatggt tgctattttt
    21841 agtaaatggt tggcagcatt ttttacacga ttgattttta agtattctaa ggctcaaggt
    21901 cagttgattt ttggtttaag tagtgctcat gcagctgcaa ctttagctat tatcttagta
    21961 ggttaccaag ccggaatttt agatgaaaat attttgaatg gaactattat tttgatttta
    22021 attacttgta ttgtggcctc atttgcaact gaaaaagcgg caaaaaccat aattgtagag
    22081 tcagaaagtg atgtttcaga attggtaaaa agtgctgaaa ttcaaaatga acatatttta
    22141 tttccgattg taagtgcgac aaacattgaa aaagtattag agtttttaat tttaattaag
    22201 gataagaaat ctgttcatcc aatttctgtt ctttcagtag tttctaatga tgcggaggct
    22261 gaaattaata ttttgaaatc caaaaataaa ctagaagaat atgtaagaca agcatctgct
    22321 tttgaagcta aagtcaatat cattaccacg atagaccata atccatcaag cggcattagt
    22381 cggatagcac gtgaaattat ggcggatatc attatgttgg gttggcctcg tcaatatgga
    22441 tttgtcgata aaatatttgg gaaaaaatta gataaccttt taaggtcgtc tgataaaaca
    22501 atttttgttg gtcatcttga aaaaccttta gccttactca aacgtattgt gataattgtt
    22561 ccacctttgg ctgagtttga aaatggtttt acactttggg ttatgaaaat ggcaaaatta
    22621 gctcaagaat taagtattcc aatggtggta tatagcaatg aaacttcaaa aattgctgtg
    22681 gagaaagtat tctcaaaaga aaaattatct gctaaattat atacctatct ttttatagat
    22741 tgggatgatt ttttggtgct ttcaaaatac attcaagaag aagatttatt ggttttggtt
    22801 tctgcacgaa aaggaacgac atcgtatatg agaattttgg aaactattcc actaaagttt
    22861 gagaagtatt ttgcaataaa tagtcgattg gtaatttatc ctcaagaata tgatacagca
    22921 attatcgaac gatttgaaga tatttcaggc gaacctttag atcgaggtat cgaagctgtt
    22981 caaaaaattg gtaaaggtat aggttcaatt ttcaaaggaa atgaccagtc ctagtttttt
    23041 attgatgtag tttttaatta aattctattg gctgattcaa attgaatgag tgtaaaaatt
    23101 ttgattttta attttgattt tatacattag acaaaaattt taatatgagt attataccag
    23161 ttttggattt agcagattat ttgtctgatg atataacaag aaaacaaaag tttattcaag
    23221 attttggaaa agcttatgaa gagataggtt ttgcagcgat tgaaaatcac ggaataccac
    23281 ctgaattaat tcagcgattt tatcagtcta tacaggattt ttttgcattg ccaactgaag
    23341 aaaaattaaa atatgaaatt gtagaattgg cgggacaacg aggttatact tcatttggta
    23401 gagaaaaagc taaacaaagt gaagtagctg atttaaaaga attctttcaa tttggacaat
    23461 atattgaaga tggagagaaa atgcctgaag aagattatcc cgataatgta gaagttaaag
    23521 aagttgaaga ttttaataaa attggaaaag aattgtatca aaattttgaa aagtcaggca
    23581 gacatttatt gagagcaatt gctgaatatt tggaattaga taaaaattat tttgattcta
    23641 aaattcataa tggaaacagt attttgagag caatttacta tccaccaatt acacaagaac
    23701 ccaaaaatgc tattcgtgcg gaagaacatg aagacatcaa tttaataacg cttttagtag
    23761 gtgcaagtgc tgatggtttg caagttttgg atagaaataa tgtttggcat gatgctaaag
    23821 cggaaccaca tcaaattatg gtaaatgtgg gcgatatgtt acagcgttta tgtaataata
    23881 aattacgttc taccacgcat cgtgtagtga atccgccaag agaaaaatgg cataccccaa
    23941 gattttcaat tccattcttc ttgcatccta gaagcgaaat gcgtttagat tgtttagaaa
    24001 attgcatttc agcagaaaat ccaattgcct ttgagcctat ttcggctgga gaatatttga
    24061 atgaaagatt gagagaaatt ggtttgaaga agtaaacaat caagttggtt atcttttgat
    24121 aaaatcatct attctattag cttttcaata ggaaaatctt tatttttgtt tctatgtaca
    24181 aaacaacaca tttacataaa attccaaatt cttttgaatt atattctaga attgcaactt
    24241 cagtaataaa aaagcccaaa gcattcttac ccaacttaga agttagaatt gatgatttaa
    24301 gtattgctca aaaagaaacg aatgcttaca atagtttgtt aggtttcgga ttgacgaatg
    24361 ctgtgccatc aactttctta tttgttaaat cttttaatct taaaacatat ttaatgagtt
    24421 cattagaaat gccatttcct atgttaggta tggtgcattt cgccaataaa atcaaacaaa
    24481 ttgaaccttt aaattataat caaaaatttt ctattgtttg cagaactgga aatttaattg
    24541 cacattctaa aggtcaagca tttgaaatta atacaagtgt cgaggtaaat ggtaaatcaa
    24601 tatgggaaga aacgatggtt tctttgagta aaggcaaaga gggaatagga gaggttttag
    24661 agtgggatat tcaagctgtt gatgctaagc atgaacgtag agtttggaac tttgaagata
    24721 atttaggcat taaatatgca caagcatcag gtgatttcaa tccaattcat ttacatccaa
    24781 tttctgctca gttgtttggt ttcaaaagac atatcattca cggaatgtac acagcaggaa
    24841 aaattttagc acaatatcct aaggtttgga atcaacccca tcagtttgcc gcatctttta
    24901 aaacgcctat ctttttgcca agtacagttg tttttagaag ctccgaagcc aataataaaa
    24961 taactttaga tgtagttgat aaaaatgaaa ctcagcctca tttaaaagga tacattcaat
    25021 attaaattga aagacttttt taaaaaagaa gcattttata cagtcaatac ctttcctgaa
    25081 tttaggtatt atgtcatcgc aagattctta ctaactatgg caatagcttt tcaatccgtt
    25141 ttgattggtt gggaagttta tttattgaca gatagtaaac taatgttagg ttttattggt
    25201 cttacagaag ctataccagc gattggattg gcactttatg gcggtcatgt tgctgataaa
    25261 tctgaaaaga aaggtttgtt ttttaaatgc ttaatttttt atattctatt aagtgcaaca
    25321 ctaatattat taacacatag agattttatt agttattttg gaaaaagaga tgtcatcata
    25381 ggcatatatg ctgttatttt tatgacagga atggtaagag gtttttcggg tccagcaggt
    25441 tttgctttag caactttttt aattccaaaa aaatattatc caaatggagc aacttggacg
    25501 agtggtgctt ggcaattagg tgctattatt ggttcagctt tagcaggttt attatatgga
    25561 atattgggaa ttactttaac atttaccttg caactttttt tattggtttt atctacgttt
    25621 ttaatctcta agattcaacc aaagccaatt attaataaag atatgactga aaaaatctgg
    25681 gatagtgtga aagttggttt gcgttttgtg tttcaaaata aaatcattct ttcttgcatt
    25741 tctttagatt tatttgccgt tttatttgga ggagcaacag cattattacc tgtttttgct
    25801 aaagatattc tgcacgttgg ttcatttgga ttggggatta tgcgagcaat gcctgcagtt
    25861 ggtgcaatca tagtcatgat ttggatggca ttttttcctt ttaagaaata tgtagggaat
    25921 attttgttga ttgctgttgc aggatttggt gtttttatta ttctttttgg gttaagtgag
    25981 tattttattt tgtctttatt ttgtttgatg atgagtggag ctttagatgg cgtgagtgtt
    26041 gttattcgac aaacaatttt acaactaaaa acacctgaag agatgcgagg gcgagtagca
    26101 gctgtaaatt ctatgtttat tggttcttct aatgaaattg gtgagtttga aagtggcttg
    26161 gcagctaaat tattaggcac tgtacctgcg gttatttttg gtggagcaat gactattttt
    26221 gtagttattg taacctattt taaagcaccc tcactcagag caatgcatga gttaaaagct
    26281 gatgattaaa tctgaattct ataattggaa aaaggttgtt ttttttcatt ttacttgtta
    26341 ttattcttac acattttttt tagttattaa tgtgtaaatc tattttagga caagacaaat
    26401 tccaattgtc cgattgaaat ttgtttgttt tgttgtttgt caatgatgta gtcgttaatg
    26461 caatttgcta caaattcccc caatttttca ggtactgcat taccgataat ttgttcaagg
    26521 tcggtttttg taccattgaa aataaaa
//
LOCUS       DAOQCW010000019        26492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_680_length_26492_cov_5.541514, whole genome shotgun sequence.
ACCESSION   DAOQCW010000019 DAOQCW010000000
VERSION     DAOQCW010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 26492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26492
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_680_length_26492_cov_5.541514"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="PLS16_03010"
     CDS             <1..926
                     /locus_tag="PLS16_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HNJ60079.1"
                     /translation="LRRGQSDCAPERDNDCIACLGGCHRDSKQSSLVAFVQKAEKTGM
                     DIKAEVEIGKVEPNADTCKVYGTHQGNKLMFEGKKVILSGGAFGSTKMMLQSGYKSKL
                     PMLGQKFASHPQFMWFGFQKDYVDAHKGMFQNVASKDPNFRKEGFKLENVFASPVSIG
                     MLFGDVGKKHQDYLKRYRHMQCIEVAVRDENVGEIRVDNQGKMIIHKPLTDQDNIRKN
                     KGLDVIRKVLHASGAEEIYESDLYFGLHLMGGCVMGVDATKSVVDPEFRVHGEKTLYI
                     ADSSIYPNAPGINPALTIMSLGNRLANQLTK"
     gene            1014..1415
                     /locus_tag="PLS16_03015"
     CDS             1014..1415
                     /locus_tag="PLS16_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60080.1"
                     /translation="MNKFSNRAIKGICKVGDVYLPQNGEFPKYSDVGGTQFLDMMIEN
                     APPEDIKALNLAMAIISFLPNFMVAWLVNWFASATKSSSNGIIASNLRQLHMGLRGLV
                     YGTYYSELINPNYKGKTPLELLDYSINRVED"
     gene            2136..2390
                     /locus_tag="PLS16_03020"
     CDS             2136..2390
                     /locus_tag="PLS16_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60081.1"
                     /translation="MNLNYWLKDKTIEIIELQVIDKNISYEKATDYYIVFNSNYGRLK
                     NKVSRRNYKSFLIGEKYKASVNKGFIEGYFLTEPFNKIND"
     gene            complement(2490..3038)
                     /gene="infC"
                     /locus_tag="PLS16_03025"
     CDS             complement(2490..3038)
                     /gene="infC"
                     /locus_tag="PLS16_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNJ60082.1"
                     /translation="MIPKRRNFPPIKKKQTEHRLNKDITVAEVRLVGEEIEPGVYSIQ
                     KALELAKELEVDLVEISPKAVPPVCRLVDYNKFLYDKKKKDKEIKAKQKKTEVKEIRF
                     TPNTDDHDLGFKLKHAISFLQEGSKVKIYVQFRGRAIQFKDRGELVLLQFADALKEYG
                     SLEAMPNLEGKKMIAYIAPKKK"
     gene            complement(3077..5002)
                     /gene="thrS"
                     /locus_tag="PLS16_03030"
     CDS             complement(3077..5002)
                     /gene="thrS"
                     /locus_tag="PLS16_03030"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNJ60083.1"
                     /translation="MSIKITFPDGAVREYDKGITALDIANKLSNSLGKKILSAKINNK
                     VCDATLPINDDATLELLTWNENDGKQTFWHSSAHLLAEALESLYTGVKFGIGPPIDNG
                     FYYDVDFGDNVVSANDLVKIEKKMKDLAAQKSEYKRQEISKADAIQYFTEKGDEYKLE
                     LLDGLNDGEITFYTQGNFTDLCRGPHIPNTGFIKSIKLMKIAGAYWRGDEKRKQLTRI
                     YGVTFPNQQELDDYLLMLEEAEKRDHRKLGQQLQIYTFDEEVGPGLPLWLPNGGFLIE
                     KIEELAKKTEDEAGYSRVRTPHIAKEALYLRSGHLPYYAESMFPPMELDNVKYYLKAM
                     NCPHHHKIFGAIPKSYKDLPIRLAEYGTCYRYEDSGSLFGLMRVRSLQMNDAHIYCTK
                     EQFEQEFRAVNEMYLKYFKIFGIEKYKMRLSKHEPSKLGQKYINEPELWIETEKMVRD
                     VLISSNIPFDEVPDEAAFYGPKIDIQIYSVIGREFTLATNQVDFAQGRRFNLKYVNKE
                     NQEDYPIIIHRAPLSTHERFIGFLLEHYAGNFPLWLTPKQVAILPISDKFLDFSTQIA
                     NELKANGFRITLDERAEKIGKKIRDAELMKIPYMLVIGEKEVENQQISVRKHGKGDIG
                     TFTIQDFNLLLQKELEN"
     gene            5202..5579
                     /locus_tag="PLS16_03035"
     CDS             5202..5579
                     /locus_tag="PLS16_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60084.1"
                     /translation="MQKLLFIYIALIIFIRPAVPAFVYFLNYDYISKTLCENKNKPEE
                     HCNGHCYLNKNISKDIQDNNKGTSNTKSIEVIDFIAICDYQKWLSPTSIFSTKDCQNF
                     ENYSDNYVFNYCSRFFHPPTTLI"
     gene            5663..6487
                     /locus_tag="PLS16_03040"
     CDS             5663..6487
                     /locus_tag="PLS16_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60085.1"
                     /translation="MKKIIIMAIAIFSLALISCEKETNENKIKKVQEINDDNYIVELY
                     TETGKFQTGYNEIFIKVKGKNTDNYIENININWYPEMQMMDKKHSCPRSSVEKVAGET
                     FLQKGYIIFQMAGNTMEHWNLTITLNDGEQTVITEQINVQAATYKNVATFKDTISGKK
                     YVIALIQPQKPTVATNDLSFGLFEMESMSNFLPVTNFTILFDPRMPSMENHSSPNNTQ
                     PSYNNVDGLYHGKLSLTMTGEWKLNLIIKNAVGDIIQGNAVSETEDSNLYLMLEAK"
     gene            6553..8604
                     /locus_tag="PLS16_03045"
     CDS             6553..8604
                     /locus_tag="PLS16_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ60086.1"
                     /translation="MKKIIFILLFFLSFISLLAQDDITDSIPEIKELDEVIVLSKTSK
                     NVGKQFKTMSTLDEYLEKSSKITLIRRGGYAWEPLINNMSSERLNITIDGMHIFGACT
                     DKMDPVTSYVDVSNLSEINVSSGQSGASNGPTVGGGLNLIRTQHDFCNPGWSGALDFG
                     IESNSGLVSTGINLKYTHSKFFIDADFTYRNADNYKAGKNVLVKFSQFTKYNTSVNLG
                     VKVNEKNSISASYIFDNAINVGYPALPMDVSLARASIANIQHSAFDLNNFIKEWETKV
                     YFNTIQHVMDDTKRPSVPIHMDMPGWSTTYGLNSRMEALKNKHKMSFNLNAYHNQSKA
                     EMTMYPNNKEEPLMFMYTWPDIRTTYVGISVKDRVQLTENHNIQYALSAGYHQNYVAN
                     EFGLNSLKIFYPNMQASKKRGLYSTQIGYTFNKRNFELNTGFAYGERAPSVSEAYGFY
                     LFNSFDKYDYIGNPNLKNEQSIESNLGLTYKISRFKANVSATYFHILDYIIGKHNPKY
                     TPMTIGATGVREYSALKSVNILSTDLSLEYRPINALKTRAFFSYSYGKDHQKQQLPLI
                     RPFSFNMGITYLIKSVDLELAIDGASKQYTYSKQYGESETPAFAILNFYGGYVLEKPN
                     YKLQLRFGVENILNTYYSTYSDWNHIPRKGRNFFISISYQFKKSKKEISEPEDHLGHI
                     H"
     gene            complement(8615..9517)
                     /locus_tag="PLS16_03050"
     CDS             complement(8615..9517)
                     /locus_tag="PLS16_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60087.1"
                     /translation="MINIKQMLFISLVALFCMSAAEKQKIDLGLAVKNNAISISMLGN
                     ENSTHYYEPVLANMNNNSMDYLEINMPAGTLLEPANETEQTLIVTEDLIVDLSPKQSK
                     MVKVKAMCIEPWDRAGGVASIYSLKTNKNDTLIKLAKYISANKYFTSCGQNAVWTFVQ
                     RDRLAEIYGSDTIEQNSLRLFLNKLTGMPMPKPEELNNYRYNYYVPPKETLSGYFQFG
                     MQSPHDIQVAMFDTTGILVRELFNQKQYQPSNGQKIKYEFDFTVYQNDKYFVKLIVDN
                     EVIMTRPINAKSFRDKYKQLYEQR"
     gene            complement(9555..10493)
                     /locus_tag="PLS16_03055"
     CDS             complement(9555..10493)
                     /locus_tag="PLS16_03055"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HNJ60088.1"
                     /translation="MIPLKLDLPILHTDIKLNFKNKLFFIGSCFAENIGNYLKTNSFD
                     VEINPFGIVYHPIAIFNEIQQLMDNRIYTEKDLVFNQNLWHSWEHHGRFSHLDKTICL
                     EQINLSIEKARTHLKDTKYLFLTFGSSIVYELLEQQKIVSNCHKFPSNQFKKYFLSLE
                     PIQQAFDTFYRNIKAFNPDINIICTISPVRHWRDGLIENNKSKSRLIYCVHDWIEKYK
                     NVSYFPAYELILDDLRDYRFYERDLCHPNQMGIDYVIDYFQQMYYDEPTKLYVKEIEA
                     YLKFKQHKPQFEQEKHYKKLEALKFELERRFQMVIQ"
     gene            10626..11783
                     /locus_tag="PLS16_03060"
     CDS             10626..11783
                     /locus_tag="PLS16_03060"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HNJ60089.1"
                     /translation="MQLFPSGKPLLMPIIGLNSGETLELHFDDLSMNNHAFYYTVVQC
                     HTDWTPTILNPMEYINGFIESTIFDYDYSNSTKQEYIHYKMEFPNNDMKITKSGNYAL
                     LVYTDNMQNPVFTYRFMVVESLVSIDAKVAYPRNYYDREKYQDITFSINNNNFTINNP
                     QAEIKATVLQNNRWDNSIENVPPYMVGFKSINYDYSNKFLFPSGREFRQFDLRSLRFK
                     GQGIRYLDVAKNENQVYLSFDQVMMNQKYNFFQDLNGSYYIHTFDYPNYLTESDYSWV
                     QFYLDYKQPLALGKVYVVGAFNNWICDANSQMHYSENEAAYTANIFLKQGFYNYAYVY
                     EDENGNKDFFQMEGYYYNTENDYTILVYYTPLGERYDRLIAVRSINSIRNR"
     gene            11889..12242
                     /gene="gldC"
                     /locus_tag="PLS16_03065"
     CDS             11889..12242
                     /gene="gldC"
                     /locus_tag="PLS16_03065"
                     /inference="COORDINATES: protein motif:HMM:TIGR03515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldC"
                     /protein_id="HNJ60090.1"
                     /translation="MGKTSEIKFIVNLDEQQMPENITWSASDSTNQTGEPCKSMMIAL
                     WDGNEKNTLNINLWTKDMQVDEMHTHFFQTLLNLGDSYFRATANPYVREEIKQFCMDL
                     ADKTRAWEESERKDV"
     gene            12235..13269
                     /locus_tag="PLS16_03070"
     CDS             12235..13269
                     /locus_tag="PLS16_03070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNJ60091.1"
                     /translation="MFDFLIIGQGIAGTMLAYQAQQKGFSVCAFDTHNPNSASNVASG
                     VVNPITGRKMLKTWKADTIIPYAIDTYKAIEKELDIKFIHEIPIAKIITSEEDVNLWN
                     SKLEDSRYQNNISPIQSLNNPHINEAFGVGFIQPTFWMNMKVMVQKIRTNLLKNNLLF
                     EENFNYSLIEFDDTIKYKNIEAKYIIFAEGFNIQNNPFFNFIPFKPAKGEQFKIYSKE
                     LKLDYIINKNIFIIPLGNDTYHVGSTYIWDDETEMVTEEGRKEVLTKLDKIVKCKYEV
                     IDEKAGIRPTIKDRRPVLGAHPEHKNMYVYNGMGTKGISLAPYFSNELILHITENKEI
                     DKEIGIQRFI"
     gene            complement(13288..14019)
                     /locus_tag="PLS16_03075"
     CDS             complement(13288..14019)
                     /locus_tag="PLS16_03075"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HNJ60092.1"
                     /translation="MHFDLEAYNDFPVAVKVFVENRKNSRVSITQQNIIVRLPKFLPD
                     YEKEKRLKAHLDWAKEVIEKKALYQKHQKKLEDYHQKNLEIFQMDFYLTVFEIETGKN
                     KLSYKGNGNIEFYLLKTLDEKVKVSVIQNLLSKFTTKYFISKISERTFYLNDLYFKEN
                     IKAIKLNYTTSKWGSCSSKRTIVFSTKLLLLPSNVIDYVIVHELAHLKEMNHSPNFWK
                     WVESAMSDYKKWDKWLNKNGKDFDF"
     gene            14153..14449
                     /locus_tag="PLS16_03080"
     CDS             14153..14449
                     /locus_tag="PLS16_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60093.1"
                     /translation="MKQFLSSLLLAIVLILSVSACQNNCKKGDDACCSSKKECCDKKE
                     CKEGKCTPEDKCCDDCSGEPSKGKECCVHDAAATDSATTNVTTADTTVTTEVAK"
     gene            14545..17145
                     /locus_tag="PLS16_03085"
     CDS             14545..17145
                     /locus_tag="PLS16_03085"
                     /inference="COORDINATES: protein motif:HMM:NF027637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HNJ60094.1"
                     /translation="MRITTTIIIIAIAILTFQTTIAKEKKEKKKKKGEVAMMTEDTIP
                     AIDSNKIDKEGFKPIKELTKKCSKISGLFNLYQDSASGKLYIEITEDKLGKEFIYFAQ
                     TVNAVIDAGFLKGGYLDEKIIKVNRYFDRLDFTIQNTDYYYDPSNPLSKSKNSNINQP
                     LFLSEKILAKTGKAYLIDADNLFFSENIMQIKPTPEPIPGVKPFTLGSLNRSKSKYLS
                     IKNFPNNSLVAVSYVYDNPSPSNYGKDAVTDARFVTIEIAHNLIAMPQNDFQSRSDDP
                     RVGFFMSKRTDQTSTSPTPYLDVIHRWDLKKKNPEQALSEPITPIVWWIENTTPKEFR
                     PTIKEAVLAWNKAFEKAGFINAIEVNEQPDNATWEADDIRYNVLRWTSSPNPAFGGYG
                     PHFANPRTGQILGADIMLEFIYLTNRVIYEKLFDVAGLTETEETTLHSNHSCQFGQQM
                     HENILAGLSVMNANDFSDLEQREFMKQALFNLVLHEMGHTFGLNHNFIASQVLSPDEM
                     KDIKISTEKGLTSSVMDYTIPNISSDKNKQGLYFDHKPGMYDTWAIQYGYTPFKDTID
                     EQNGLAKILSESTQKEHLFFNDGDDMRYPGKGIDPRVMISDMSSDAIAYATENTEQMI
                     STMGKLLDKYKNENQSYYPIRNAFTVLTSAMSRNFGVVSRYIGGIYIDRSFMNQNSPN
                     KPYTPVPLQTQKAAMKMLRDYCFGPEAFSKSTEVYNYLQYQRRGYNRDGNEDPKLHER
                     FLNIQKGVLDQLLNTNVLQRIEDTKLYGNQYDLNKMLTDLTNAIFIEGFSNPSTIRQN
                     LQGEYINRLLNIIDEKSGNTYSVKSAVYGQIDRVKKLLEVSASSGSLAVNAHRQNLLY
                     LINNKLSIKN"
     gene            17206..18225
                     /locus_tag="PLS16_03090"
     CDS             17206..18225
                     /locus_tag="PLS16_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60095.1"
                     /translation="MKRNQSKICIVLILFSISVFFSACKKDKKQETIDVYVAVDEFNG
                     TFNIAKLWKNGIIQDLNDGTKSASASSIFVQGSDVYLVGFEENSLGTNVAKLWKNGVA
                     QNLSDGAFDNFANSIYVANNKTYIAGYQDNGIKRVAKIWVNGVATNLTNGLNHAYAGS
                     VFVSGDDVYVGGYEENIIGISIAKIWKNGVAQSLTDGVHNASVNAIYVQDNDVYAVGY
                     NGGTDIVWKNGIPQNLTDGSVYAYANSIFVLNNTVYVVGHKNNGTHNVATLWKNGVAQ
                     YITDGSKKCSASSVFVLDNDIYIVGYEENGSKDVAKLWKNGVAQNLTNGTNDGSANSV
                     FVSKK"
     gene            complement(18247..19122)
                     /locus_tag="PLS16_03095"
     CDS             complement(18247..19122)
                     /locus_tag="PLS16_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNJ60096.1"
                     /translation="MKSFFKFLIHSNIFISIAAVCFTMANALLLYIEVDHILLLLVHI
                     FFSTWFIYQFSRLLYQQKMNEKNTEDDIYYYQIKHQKITKITTALSLIISGITFLFLK
                     FPTQVAIVFSGFLSVVYALPLDKIGIKKRLRDIPFVKIFLIAITWTITAVLLPILETN
                     TYPDLFVRRVVLLFSIQFIYLLFITLPFDINDIERDVANQTKTIATTLGARKSQMLCV
                     ALFLIDSYLLYIFRVSTEIHIFFNLLLISLIAISVYSSHKISKTWIMFFYDGSMIAYF
                     IGIFVLMTLETRAYL"
     gene            complement(19174..20445)
                     /locus_tag="PLS16_03100"
     CDS             complement(19174..20445)
                     /locus_tag="PLS16_03100"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HNJ60097.1"
                     /translation="MYYWAKVYIFISFLLGGYQLSIAQQRKCGYDNYYQNILKEYPEI
                     VEKEIALQNELKNNNQRYAQSEGIIRIPVVIHVLYNTESQNISDEQIKSQISALNRDY
                     RALNTDKLSSNHQFYDRIGDTEIEFCIATEDPWGQITTGIERKYVSKSSFSYSGNLND
                     ADENYIKKSNKGGLDAWDTDKYLNIWIANFTDGTLGFASFPNDNAGIKDGIVIHYRVF
                     GTIGNLLPDYNKGRTTTHEIGHWLGLKHIWGNGGNCNSDDGISDTPKQYDATYGCPSG
                     IYDDECSPSTSGRMYQNFMDYTDDACMALFTKKQAEYVRNVLNSSRSSFLNNNIPCDF
                     ISKVTNNTLFKNIKVFPNPATNYLYIDQLPSSKEPYRIALYNELGQAVFTTTTNDYNT
                     EIEVSELRKGVYYVEIVNGEHHYNQTVLVLH"
     gene            20561..21019
                     /gene="lysM"
                     /locus_tag="PLS16_03105"
     CDS             20561..21019
                     /gene="lysM"
                     /locus_tag="PLS16_03105"
                     /inference="COORDINATES: protein motif:HMM:NF008399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HNJ60098.1"
                     /translation="MGLFNFIKTAGQKVFGKKPEETQTEEEKQLQAKELLAYVQSLGL
                     EISNLTVIVSGQKVTVRGEVASQEIREKILLALGNVEGVAEVDDTISVIVPAPEAKLH
                     TVVSGDTLSKIAKTYYGNANDYNRIFEANKPMLSHPDKIYVGQVLRIPQA"
     gene            complement(21082..21405)
                     /locus_tag="PLS16_03110"
     CDS             complement(21082..21405)
                     /locus_tag="PLS16_03110"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNJ60099.1"
                     /translation="MNWYLLDSDIQQLMIDAINYKPFKIVFNGKPICIVKTEEEIFGI
                     SNICPHAGAELHHGFANKRLVIGCHLHGYKFDMKTGLSADGNQYKIPRYRFKLEDGNY
                     FISAV"
     gene            complement(21453..22352)
                     /locus_tag="PLS16_03115"
     CDS             complement(21453..22352)
                     /locus_tag="PLS16_03115"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HNJ60100.1"
                     /translation="MLYHNDKTILFFNGDFKLVNDFKISPFNQTLHYGYGVFEGIRAY
                     QTQSGLHIYKVRRHFERLMQSAKKLHIEMPYSVQEMINFAYELVEQNKLSEAYIRPFI
                     FMDKNMGLRAVDADKPNVLMTCWKWKKYYPNNHLNVMVSSWRRPHPKTMPVDIKASGN
                     YINSILATREATQNGYDDALLLDVEGYVATGPGASFFMEKDGKLIVPPINYIFQGITR
                     ATLIDLAKQMDIPVEEKLFGVEELLNADGAFFAGTAAEVAGIKSINGHQLKLPWEDTI
                     GRLLHDRYKRIVAGKDTNFLEYF"
     gene            complement(22446..24212)
                     /locus_tag="PLS16_03120"
     CDS             complement(22446..24212)
                     /locus_tag="PLS16_03120"
                     /inference="COORDINATES: protein motif:HMM:NF025067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HNJ60101.1"
                     /translation="MKEKFFIILNQARKVFGIYPLVLLSSFIMALITIIHITIFNFSK
                     DDSLKFLLVKLILVAGLGISLNFALKLLAQRVQQSKMIEYLGILILIGFYFILPKNEV
                     DFNEFYAFILIPIYILSHLLVAFIAFTKKENLEFSFWQFNKNLFVNFFLTLVFVSVLI
                     IGTELAILAIDNLFEIHFDSDVYWKIFLFILIFGSTFIFLSFCESGLSSIEKEDEYPI
                     ILQFFTQYILIPLLFLYVVILYVYLAKIILTWELPRGWVSYLILAYSSVGILALLLVH
                     PLKNKTEKAWVRVFGKLFYYTLIPLLILLFVAIFTRILAYSFTEPRYYVLLLAIWLST
                     VVLYFVFIKNATIKFIPISLFIFGLFALVFPYFNAISVAKRSQKNALEQILKDNKLLV
                     NGKIDFDKTITDSLVYEISSKFDFLNSRGQSAFLLSFLDDKHQVEFKNAESNYWPIES
                     KFKNVQQISNKNYPANFLQLTSKQPYYEIANYQYAILQQYPEDKNININQDSIQIKTL
                     QNSALYMVLNNKDTLNLKPLAIKLFDNYPNNPDVLSIEGQLANYKIKVIFGTIEKTNV
                     DANRIDFWFSNVVYLIDDTNKK"
     gene            24287..25210
                     /locus_tag="PLS16_03125"
     CDS             24287..25210
                     /locus_tag="PLS16_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase C
                     domain-containing protein"
                     /protein_id="HNJ60102.1"
                     /translation="MKKLILVMCIALVLGYACKKNDGTNFEPIKPPKDTTIIIDTPKV
                     DTPIVVEPPYFNRKYNKVCFLMTHNAMNNSEKKYLIPNQTHSISKQLANGVRGLMIDT
                     YDGPGGNALTYHGVELMGKQPLVDVLKEVYLFLANNEKEVLTIIFENNGSNEQLIAAI
                     ETAGLTSFAYVHGTEWKTIKEMVNSNQRLVMFVESDKTPKVDYLKHAWSYIYDTKYTF
                     KKVSEFDCAINRGTYSENNLFLVNHWLSNGLGLPEKKLANTANNPSVIHSRLQNCENT
                     RNHFINFLGVDFYEVGNTKAIVDSINSHFVE"
     gene            25285..25587
                     /locus_tag="PLS16_03130"
     CDS             25285..25587
                     /locus_tag="PLS16_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNJ60103.1"
                     /translation="MNIYIANINFRTSTEALQQLFENYGEVTSAKIITDRETGRSRGF
                     GFVEMSNDEEAQKAIDELNGSEFDGKALSVNVAKPKENNSRGSYGGGNSNYKKKSW"
     gene            complement(25655..>26492)
                     /locus_tag="PLS16_03135"
     CDS             complement(25655..>26492)
                     /locus_tag="PLS16_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60104.1"
                     /translation="GFLSFIFFATGFITAVAGIVADRYTFMALPWFIILFVFVLTHFL
                     PIQNIYTKGFFVFMIVLLTTASFVRTQAWESLTTLVETDAPKLHNSYEGMRIATNVYI
                     DNFKETNDTSYLNKGIQCALNANQVYENNVLINTQLGQLLFLKNDLSNAEKYFKMAFA
                     IDSTNASLLEFLGDVYYSKKDYKNSENYYLGAYQFATDRHKQALINNISTLYYEQGFK
                     EKTLNYNFDLIRKDSLNFAAYENLGYYYLSERDTNKARMYFNLAVKFGMPSEGFPELS
                     NK"
BASE COUNT         9729 a         3946 c         3829 g         8988 t
ORIGIN      
        1 atttaagaag aggtcaaagt gattgtgcac ctgaacgaga taatgactgt atcgcttgtt
       61 taggtggctg ccatagagat tctaagcaaa gttctttagt agcttttgtt caaaaagccg
      121 aaaaaactgg tatggatata aaggctgaag tggagattgg aaaagtagaa cccaatgctg
      181 atacttgtaa agtttatggt acacaccaag gtaataaatt aatgtttgaa ggtaagaaag
      241 taattctttc tggtggtgct tttggttcta ccaaaatgat gttgcaatca ggttataaat
      301 caaaattacc gatgttagga caaaaatttg cctcacatcc acaatttatg tggtttggtt
      361 tccaaaaaga ttatgttgat gcacacaaag gaatgttcca aaacgttgcc tcaaaagatc
      421 caaacttcag aaaagaaggt tttaaattag aaaatgtttt cgcttctcct gtttctatcg
      481 gaatgttgtt tggagatgtt ggtaagaaac atcaagatta tttaaaaaga taccgacaca
      541 tgcagtgtat tgaagtagca gttcgagatg aaaatgttgg agaaataaga gtggacaacc
      601 aaggtaaaat gattattcat aagcctttaa ccgaccaaga taatattaga aaaaataaag
      661 gtttagatgt catcagaaaa gtgctacatg catcaggagc tgaagaaatt tatgaatcag
      721 atttatattt tggcttacac ttgatgggtg gttgtgttat gggtgttgat gctacaaaat
      781 ctgttgtcga tccagaattt agagtgcatg gagaaaagac attgtatatt gcagattcca
      841 gtatttatcc aaatgcccca ggcattaatc ccgcattaac gattatgtca ttaggaaacc
      901 gattagctaa ccaattaacg aaataattat agattatatg tattgaatag atttgtaagc
      961 ttgaaaaact atcctactta ctactcaata ctcactactt aatacttaaa aagatgaata
     1021 agtttagcaa tagagcaatt aaaggaattt gtaaagtagg tgatgtttat ttacctcaaa
     1081 atggagaatt tccaaaatat agcgatgtcg gtggtacaca atttttagac atgatgatag
     1141 aaaatgcacc acctgaagat attaaagcct tgaatttagc aatggctatt atttcatttt
     1201 tgccaaattt tatggttgct tggttggtga attggtttgc ttcagcaacc aaaagttcca
     1261 gtaatggaat tatcgcatca aatttacgac aattacacat gggattacgt ggattagttt
     1321 atggtactta ctattcagaa ttaatcaatc caaattacaa aggcaaaaca cctttagaat
     1381 tattagatta tagtataaat agagtcgaag actaaaacta aaaaagatac ttttcacaag
     1441 gcgtttcaat ctggaacgcc tttttttatt tgttttatgc tgaaatttgt ttgcatatag
     1501 ataactccta caaaattatc acttttattg agtggtttaa aaaaaatgta tttcctttca
     1561 tatctataaa ttttgtaaat tttaaagata aaaaacatat atttgagagt tgtaacgtaa
     1621 cttgggagaa cgatcaccgt aggtgtcgct tgaacatctg ttttgcaaga tagcgggtga
     1681 agtgctacta ttgttcagaa gtgtaaattt gaacggtagt aattctgcct ctgacgggtg
     1741 ttattacctg tcagcaaata aatatgaaaa agttaagata gaataattaa gaaaaaggaa
     1801 ttttagacaa aaggtgataa tacctttttg agtgtaagaa aaaaccaagt aaagtaattt
     1861 ttgtattaaa ataaaatgta tatttagagt aggatttaag caaaaaactg taaactatat
     1921 ttaggacgat acttttatct tgacaaaggt tttagtgtaa gttttacaac gataacggca
     1981 ttggctctct tccatatagt tttaatcata attctcaaaa gactagataa aattgaatta
     2041 gatttaccaa tatctaattt aaaaaaaaca ttttcaatca ttgtatatgt attactatta
     2101 tttctttttg ttttttgtta gaaatgggtt tacgaatgaa cctcaattat tggttaaaag
     2161 acaaaacaat agaaataatt gagttgcaag tcatagacaa gaatatttca tacgagaaag
     2221 caactgatta ttatatcgtt tttaactcaa attatggaag attaaaaaac aaagtaagta
     2281 gaagaaacta caaatcattt ttgattggag aaaaatataa agcttcagtg aacaaaggct
     2341 ttattgaagg atattttttg acagaaccat ttaataaaat taatgactaa tgagaaaaac
     2401 tgaacaaaac attatgattg ttctgattaa accaaaaatc actttacaac ttacacagtt
     2461 tgtgagatac taacctacct aaaaaaagtt tacttctttt ttggtgcgat ataggctatc
     2521 attttttttc cttctaaatt tggcatggct tctaatgaac catattcctt taaagcatct
     2581 gcaaattgta ataatactaa ctctcctcta tctttaaatt gaattgcacg acctctaaat
     2641 tgaacataaa ttttcacttt tgaaccctct tgcaaaaagc ttattgcatg ttttaattta
     2701 aagcctaaat catggtcgtc tgtatttggg gtaaaacgaa tttctttaac ttccgttttt
     2761 ttctgtttag ccttaatttc tttgtccttc ttctttttgt cgtataagaa tttgttataa
     2821 tccactaaac gacaaacagg cggaactgct ttgggtgaaa tttccactaa atcaacctct
     2881 aattctttgg ctaattctaa tgctttttga atggaataaa cgcctggttc aatttcttcg
     2941 cctactaaac gtacttcagc aacagtaata tctttattta atctgtgttc cgtttgtttt
     3001 ttcttaattg gcggaaaatt tcttctttta ggtatcaaat tgttttaatt atgagtttaa
     3061 aatatgatgt ttttaattaa ttctctaatt ctttttgcaa aagtaaatta aaatcttgaa
     3121 ttgtaaaagt accaatatca ccttttccat gttttcggac agaaatttgt tggttttcaa
     3181 cttctttttc tccgattact aacatataag gaattttcat caactctgca tctcgaattt
     3241 tcttaccgat tttctcagct cgttcatcta aagtaattct aaaaccatta gctttcaatt
     3301 catttgcaat ttgtgttgaa aaatccaaaa acttatcaga aattggtaaa atagctactt
     3361 gtttaggagt cagccaaaga ggaaaattac cagcataatg ttccaacaaa aatccaataa
     3421 atctttcgtg cgtactcaat ggtgctcgat ggataattat tggataatct tcttgatttt
     3481 ctttattgac atacttcaaa ttaaatcttc taccttgagc aaaatctact tggttagttg
     3541 ccaaagtaaa ttctctacct atgacagaat agatttgaat atcgattttt ggaccataaa
     3601 acgccgcttc atcaggtacc tcatcaaatg gaatattgga gcttatcaat acatcacgca
     3661 ccattttttc agtttctatc caaagttctg gttcattgat atatttttgt cctaattttg
     3721 atggttcgtg cttacttaaa cgcattttat atttctcaat tccaaatatt ttaaaatact
     3781 ttaaatacat ttcgtttacg gctctaaatt cttgttcaaa ttgctctttt gtacaataaa
     3841 tatgagcatc attcatttgc aatgaacgca cacgcattaa tccaaataaa cttccagaat
     3901 cttcataacg ataacaagtt ccgtactcag ctagtctaat tggcaaatct ttataagact
     3961 ttggaattgc tccaaatatt ttatgatgat gagggcagtt catcgctttt agataatatt
     4021 ttacattatc taattccatt ggtggaaaca tactttcagc atagtatggt aaatgcccac
     4081 ttcttagata caaagcttct ttggcaatat gaggtgtacg cacacgagaa tagcctgctt
     4141 catcttctgt ttttttggct aattcttcaa ttttctcaat taagaaacca ccatttggca
     4201 accacaatgg caaacctggt ccaacttctt catcaaaagt ataaatttgt aattgttgtc
     4261 ccaattttct atgatcacgt ttctctgcct cttccaacat caacaaataa tcgtctaatt
     4321 cttgctgatt tggaaaagta acaccataaa tccgagttaa ttgctttctc ttttcatcgc
     4381 cacgccaata agcaccagcg attttcatca atttaattga ttttataaaa cctgtatttg
     4441 gtatatgtgg tcctctacat aaatcggtaa aatttccttg agtataaaac gtaatttcgc
     4501 catcattaag accatcaagt aattccaact tatactcatc tcctttttca gtaaaatatt
     4561 gaatagcatc agccttacta atttcttgac gtttatattc tgatttttgt gccgccaaat
     4621 ccttcatctt cttttctatc ttcactaaat catttgcaga aacaacatta tctccaaaat
     4681 caacatcata gtaaaatcca ttgtctattg gaggtccaat tccaaacttc acacctgtat
     4741 ataaactttc taaagcctct gccaacaagt gtgctgatga gtgccaaaaa gtttgttttc
     4801 catcattttc gttccacgtc aataattcta gcgtagcatc atcattaatt ggaagtgtag
     4861 catcacaaac tttattatta attttagcag acaaaatctt tttacctaaa ctattactta
     4921 gtttatttgc gatatctaac gcagttattc ctttatcata ttcacgtact gcaccgtctg
     4981 gaaatgttat ttttatactc ataattaaac tacaaaacta attatatcta tgcattaaac
     5041 aaaatagtta aaattaaatc tataaaaata atcataaaca aatttatgtt tctataaacc
     5101 ctaattacat tccaaaatta ttcctgcata aatgataaca ataagttcta ttactgacaa
     5161 atatcatttt attatcttgg cagaatcaat atttttgtat tgtgcaaaag ctattattca
     5221 tatatattgc ccttattatc ttcatcagac ctgctgtacc tgcttttgta tattttttaa
     5281 actacgatta tatttctaaa acactttgtg aaaacaaaaa caaacctgaa gaacattgca
     5341 atggacattg ttatttaaat aaaaatatta gtaaagatat tcaagataat aacaaaggta
     5401 cttccaatac taaatctatt gaagtcatcg attttattgc tatttgtgat taccaaaaat
     5461 ggctttcacc aacttcaatt tttagtacaa aagattgtca aaattttgaa aattatagtg
     5521 ataattatgt ttttaattat tgttcaagat tctttcatcc acctaccaca ttaatataaa
     5581 tttcaaccaa acacaattga gcattactta tattatagat ataagtattc aaatttatat
     5641 tattaaattt aaagaaaaaa tcatgaaaaa aataataatt atggctattg ccatctttag
     5701 tttagcgtta atttcttgtg aaaaagagac taatgaaaat aaaattaaaa aagttcaaga
     5761 aattaacgac gataattata ttgttgaatt atatactgaa actggaaaat tccaaactgg
     5821 ttacaatgaa atttttataa aagtaaaagg aaaaaataca gacaattaca ttgaaaatat
     5881 caatatcaat tggtatcctg aaatgcaaat gatggataaa aaacattctt gtccacgttc
     5941 atcagtagaa aaagttgctg gtgaaacttt cttacaaaaa ggttatatta ttttccaaat
     6001 ggctggaaat acaatggagc attggaattt aacaattacc ttaaatgatg gagaacaaac
     6061 agtcattaca gagcaaatta atgtacaagc agctacttat aaaaatgtgg caacctttaa
     6121 agatactata agtggaaaaa aatatgtaat tgctttaatt caacctcaga agccaactgt
     6181 tgcaaccaat gatttaagct ttggtttgtt tgaaatggaa tctatgtcca atttccttcc
     6241 agttacaaat ttcacaattc tttttgatcc aagaatgcca agtatggaaa accattcttc
     6301 tccaaacaat acccaaccat cttacaataa tgttgatggt ttatatcatg gaaaattatc
     6361 tttaacaatg actggcgaat ggaagctcaa cttaattata aaaaatgcag ttggcgatat
     6421 tattcaagga aatgctgttt cagaaactga agacagtaat ttatacctaa tgttagaagc
     6481 gaaataatac gcttattatt taaaatgcaa agtgcagatt tttgcacttt gcatttttat
     6541 aaattttata agatgaaaaa aataattttt atactgctgt ttttcctcag ttttataagt
     6601 cttttagcac aagacgacat aacagattca attccagaaa taaaagaatt agacgaagta
     6661 attgtcctta gtaaaacaag taaaaacgta ggcaaacaat tcaaaacgat gtccacatta
     6721 gatgaatatt tagaaaaatc atctaaaata actttaataa ggagaggtgg ttatgcttgg
     6781 gaacccttga taaataacat gtcttctgaa cgtctaaata ttacgattga tggtatgcat
     6841 atttttggag catgcactga caaaatggac ccagtaactt cttatgttga tgtatctaat
     6901 ttatcagaaa tcaatgtatc atctggacaa agtggagctt caaatggacc aactgttggt
     6961 ggcggattaa acttaattag aacacaacat gacttttgta atcctggttg gagtggtgct
     7021 ttagattttg gaatagaatc aaattctggt ttagtttcca caggaataaa tttaaaatat
     7081 acacattcaa aattttttat tgatgcagat tttacatata gaaatgccga taattataaa
     7141 gcgggaaaaa atgtacttgt aaaattttcg cagtttacga aatacaatac atctgtcaat
     7201 ttaggtgtaa aagtcaatga aaaaaattca atatcagctt cctatatttt tgataatgct
     7261 atcaatgttg gatacccagc actgccaatg gatgtttcct tagctcgtgc atcaattgct
     7321 aatatacaac acagtgcttt tgatctcaat aattttatta aagagtggga aactaaagtc
     7381 tatttcaata ctattcaaca tgtaatggat gataccaaac gcccaagtgt tcctattcac
     7441 atggatatgc caggttggag tactacttat ggtttaaact caagaatgga agcattgaaa
     7501 aacaaacata agatgtcatt taatttgaat gcttaccaca atcaatctaa agctgaaatg
     7561 actatgtatc caaacaacaa agaagaacct ttgatgttta tgtacacatg gcctgatatt
     7621 agaacaactt atgttggcat ttcagtaaaa gatagagttc aacttactga aaatcataat
     7681 atacaatatg ctttaagtgc aggatatcac caaaattatg ttgccaatga atttggatta
     7741 aatagtttaa aaatttttta tcctaatatg caagcaagta aaaaacgtgg cctatatagt
     7801 actcaaattg gatatacttt caacaaaaga aattttgaac tcaatactgg atttgcttac
     7861 ggcgagcgag ctccatcagt ttctgaggct tatggattct atctttttaa tagttttgac
     7921 aagtatgatt atattggtaa tccaaattta aaaaatgagc aatctattga aagcaatttg
     7981 ggtttaactt ataaaatatc caggtttaaa gcaaatgttt cggctactta cttccatatt
     8041 ttagattata taattggcaa acataatcct aaatatacac ctatgactat aggtgcgaca
     8101 ggtgttagag aatatagtgc tttaaaatct gtaaatattt tatcaacaga tttatctttg
     8161 gaatatcgtc caatcaatgc attaaaaaca agagcctttt tctcatacag ttatggaaaa
     8221 gaccatcaaa aacaacaatt accactaatt cgtcctttta gtttcaatat gggtattact
     8281 tatctaatta aaagtgtgga tttagaatta gcaattgatg gtgcatccaa acaatataca
     8341 tacagcaaac aatatggaga atcggaaaca ccagcttttg ccatattaaa tttttatggc
     8401 ggctatgttt tagaaaaacc aaattataaa ttacaattaa ggtttggcgt tgaaaacata
     8461 ttaaatactt actattcaac ttattcagat tggaatcaca taccaagaaa aggtagaaat
     8521 ttctttataa gcatctctta tcaattcaag aagtcaaaaa aagaaataag tgagcctgaa
     8581 gaccatttag gacatattca ttaatgaatt actattatcg ttgttcgtat aattgtttgt
     8641 atttatcacg aaaacttttg gcattaattg gtcgagtcat aatcacttca ttgtccacaa
     8701 taagtttgac aaaatattta tcattttgat aaacagtaaa atcaaattca tacttaattt
     8761 tttgtccatt actgggttga tattgctttt gattaaataa ttctcttact aagattccag
     8821 ttgtatcaaa cattgcaact tgaatatcat gtggactttg cataccaaat tgaaaataac
     8881 cactcaatgt ttctttgggg ggtacatagt aattatatct atagttattt aactcttcag
     8941 gttttggcat tggcataccc gttaatttat ttaaaaacaa acgtaaacta ttttgctcta
     9001 tggtatcaga accataaatt tccgccaatc tatctctttg tacaaatgtc cacacagcat
     9061 tttgtccaca agaagtaaaa tatttattag cagaaatata ttttgctaat ttaattaaag
     9121 tatcattttt atttgtttta agagaataaa tagaagctac acctcctgcc ctatcccaag
     9181 gctcgataca cattgctttt actttaacca ttttagattg tttaggagat aaatctacaa
     9241 ttaaatcttc cgtaacaatt aaggtttgtt cagtttcatt tgctggttct aatagcgtac
     9301 ctgctggcat attaatttcc aaataatcca ttgaattatt attcatattg gctaaaactg
     9361 gttcataata atgcgtgcta ttttcgttcc ctaacatcga aatggagatt gcattatttt
     9421 taactgcaag acctaaatct atcttttgtt tttcagctgc tgacatacaa aatagagcaa
     9481 ctaaactaat aaaaagcatt tgttttatat ttatcataac ttaagttttc acaattaacg
     9541 aaataattta aactttattg tattaccatt tggaatctac gctccaattc aaactttaag
     9601 gcttctaatt ttttataatg tttttcttgc tcaaattgtg gcttgtgttg tttaaatttc
     9661 aagtaggctt caatttcttt tacatataat tttgttggtt catcataata catttgttga
     9721 aaataatcaa ttacataatc gattcccatt tggtttggat gacacaaatc tctttcataa
     9781 aaacgataat cacgcaaatc atctaaaatc aactcataag caggaaagta gctaacattt
     9841 ttatactttt ctatccaatc atgaacacaa taaattaatc ggcttttact tttattattt
     9901 tcaatcaagc catctcgcca atgtcgcact ggactaatcg tacaaataat attaatatca
     9961 ggattaaaag ccttaatatt tcgataaaag gtatcaaatg cttgctgaat aggctctaaa
    10021 cttaaaaagt attttttgaa ttgattactc ggaaatttat ggcaattaga gactattttt
    10081 tgttgctcta aaagttcata aacaatagat gaaccaaaag tcaaaaataa atatttcgta
    10141 tctttcaaat gtgttctagc cttttctata gaaagattta tttgttctaa acaaattgtc
    10201 ttatccaaat gactaaaacg tccatgatgt tcccaactgt gccacaaatt ttgattaaac
    10261 actaaatcct tttctgtata gattctatta tccatcaact gttgtatttc attaaaaata
    10321 gctattggat gatagacaat accaaatgga ttaatttcaa catcaaagct attcgtcttc
    10381 aaataattac caatattttc agcaaaacaa gaacctataa aaaataattt atttttaaaa
    10441 tttagtttaa tatctgtatg taaaataggc aaatctaatt ttaatggaat catttcggaa
    10501 caatttttgt acattagtaa acaatgaaat taaggaaaat tatattctta tgtctgtttt
    10561 tttttagtgt tatatcctat gcaaaagttt accaaactca aatttttgat aataacataa
    10621 ggactgtgca actttttcca agtggaaaac ctttattaat gccgattatc ggattaaatt
    10681 ctggcgaaac gttagaatta catttcgatg atttatccat gaataatcat gctttttatt
    10741 ataccgtagt gcaatgtcat acagattgga caccaactat tctaaatcct atggaatata
    10801 tcaatggctt tattgaatcc accatttttg attatgatta ttccaacagc accaaacaag
    10861 aatatataca ttataaaatg gaattcccca ataatgatat gaaaattaca aaatctggaa
    10921 attatgcatt gttagtttat actgacaata tgcaaaatcc agtttttact tatcgtttta
    10981 tggtagttga aagtttagtt agtatagatg caaaagtagc gtatccacga aattattatg
    11041 atagagaaaa atatcaagat attactttta gtataaacaa taataacttt actatcaata
    11101 atcctcaagc agaaatcaaa gcaacagttt tgcaaaataa tagatgggac aattcaatag
    11161 aaaatgttcc tccttatatg gttggtttta aatctataaa ctacgattat tctaataaat
    11221 ttttatttcc ttcgggtaga gaatttagac aatttgattt acgttcatta cgtttcaaag
    11281 gtcaaggtat tcgatattta gatgttgcta aaaatgagaa ccaagtttac ctttcttttg
    11341 accaagtaat gatgaatcag aaatataact tttttcaaga cttaaatggt agttattata
    11401 ttcatacttt tgattatcca aattatttaa ctgaatctga ttatagttgg gtacaatttt
    11461 atttagatta caaacaacct ttagcacttg gtaaagtata tgtagttgga gcttttaata
    11521 attggatttg cgatgctaat tcacaaatgc attatagcga aaatgaagct gcatatactg
    11581 caaatatttt tttaaagcaa ggtttctata actatgctta tgtgtatgaa gatgaaaatg
    11641 ggaataaaga ttttttccag atggaaggtt attattataa cactgaaaat gattatacca
    11701 ttttagttta ttacacacct ttgggcgaaa gatacgatcg tctaattgct gtaagaagta
    11761 ttaattcaat tcgcaatcgt taatatatca cgctaaaaat catttagatt taatgattac
    11821 gataagtata aaaaggtcaa tttccataga ataatataaa aaagcgtact tttgtgcaaa
    11881 aaattatcat gggaaaaaca tctgaaataa aatttatagt taatcttgac gaacaacaaa
    11941 tgcctgaaaa tattacttgg tctgcttcgg atagcactaa tcaaacaggt gagccatgca
    12001 aaagtatgat gattgcactt tgggatggta atgaaaaaaa tactttaaat atcaatcttt
    12061 ggacaaaaga tatgcaagtt gatgagatgc acacacattt cttccaaacg cttttaaatt
    12121 taggcgattc ttattttaga gcaactgcaa atccttatgt acgtgaagaa atcaaacaat
    12181 tctgcatgga tttggcagac aaaacaagag cttgggaaga atctgagaga aaagatgttt
    12241 gattttttaa ttatagggca aggtattgca ggtacaatgt tggcatacca agcacaacaa
    12301 aaaggcttta gtgtctgtgc ttttgataca cataatccaa attcggcttc taatgtagca
    12361 agtggcgtag tcaatccgat tactggtaga aaaatgctca aaacttggaa agcagatacg
    12421 attattcctt acgccatcga tacttataaa gctattgaaa aagaattgga cattaaattt
    12481 attcatgaaa tacctattgc aaaaattatt acttccgaag aagatgttaa tttatggaac
    12541 agtaaactag aagattctag gtatcaaaac aatatttcgc caattcaatc cttaaataat
    12601 cctcacatta acgaagcttt tggtgttggt tttattcaac caaccttttg gatgaacatg
    12661 aaagttatgg ttcaaaaaat cagaactaac ttgctgaaaa acaatttatt atttgaagaa
    12721 aattttaatt atagtttaat agaatttgac gatacaataa aatataaaaa catagaagca
    12781 aaatatatca tctttgctga aggttttaat attcaaaaca atccattttt taattttata
    12841 cctttcaaac cagcaaaagg tgagcaattt aaaatttatt ctaaggaatt aaaattagat
    12901 tatattatca acaaaaatat ttttataatt cctttaggta atgatacata ccatgttggt
    12961 tcaacttata tttgggatga tgaaacagaa atggtaactg aagaaggcag aaaagaagtc
    13021 ttaactaaat tggataaaat tgtcaaatgc aaatatgaag ttattgatga aaaagcagga
    13081 attcgtccaa ccatcaaaga ccgaagacca gttttaggtg cacatccaga gcataaaaat
    13141 atgtatgttt ataacggaat gggaacaaaa ggcatctcat tagctcctta tttttctaat
    13201 gaattaatat tgcatatcac tgaaaacaaa gaaatagata aagaaatagg tattcaacga
    13261 tttatttaat aaacacaaca taaaccttta aaaatcaaaa tcttttccat ttttattcaa
    13321 ccatttatcc cacttcttat aatcagacat tgcagactcc acccatttcc aaaaattagg
    13381 cgaatggttc atttctttta gatgtgctaa ttcgtgaaca attacataat ctataacatt
    13441 tgagggcaaa agaagaagtt tggtagaaaa aacaatagtt cttttagaag aacaactccc
    13501 ccactttgaa gtcgtataat ttaacttaat cgctttgata ttttctttaa aatataaatc
    13561 attcagatag aaggttcttt cagaaatctt agaaataaaa tattttgtag taaattttga
    13621 caatagattt tggattacac ttacttttac cttttcatct aaagttttta atagataaaa
    13681 ttcaatattt ccatttcctt tgtaactcaa tttgtttttt ccagtttcta tttcaaaaac
    13741 agttaaataa aagtccattt gaaaaatttc taggttcttt tgatggtaat cttctagttt
    13801 cttttgatgt ttttgataaa gtgctttctt ttcaataact tctttagccc aatctaaatg
    13861 agctttcagt cgcttttctt tttcataatc aggcaaaaac ttaggcaatc ttacaatgat
    13921 attttgttgt gtaatagaaa ctcttgagtt ttttctattc tcaacaaata cctttactgc
    13981 aacaggaaaa tcattatatg cttctaaatc aaaatgcata attcaaaaac aagataagta
    14041 aaaatctatc gaagaaaaaa gtgtggttct taaactttct taaattgcag ataagttatt
    14101 gtatatatgg aaattatcta gtaattttac aaaaaatatt aatacaaatt ttatgaaaca
    14161 atttttaagt agtttattat tagctatcgt tcttatttta agtgttagtg cttgtcaaaa
    14221 caactgtaaa aaaggcgatg atgcttgttg tagttctaaa aaagaatgtt gcgacaaaaa
    14281 agaatgtaaa gaaggtaaat gtactccaga agacaaatgt tgtgatgact gttcaggaga
    14341 accaagtaaa ggcaaagaat gttgtgttca tgatgcagcc gctactgatt ctgctacaac
    14401 aaatgtaact acagcagata caactgtaac tacagaagtt gctaaataat tagttactaa
    14461 catgtttata atctaggcta ttcctcattt gggatagcct tttttattta tacttttttg
    14521 tacctttggt aaaaagttct atttatgaga attacaacaa ctattataat tattgccatt
    14581 gctattttaa cttttcaaac aactattgcc aaagaaaaaa aggaaaagaa aaagaaaaaa
    14641 ggagaagttg cgatgatgac ggaagatact attcctgcaa ttgattccaa taaaatagac
    14701 aaagaaggtt ttaaaccaat aaaagaactt acaaagaaat gcagtaaaat ttcaggatta
    14761 tttaatttat accaagattc tgcaagtgga aaattatata ttgaaattac agaagataaa
    14821 ttaggaaagg aatttatcta ttttgctcaa actgttaatg ctgtaattga tgctggtttt
    14881 ttaaaaggtg gttatttaga tgaaaagata ataaaagtta atcgctactt tgatagatta
    14941 gattttacta ttcaaaatac tgattattat tatgaccctt ccaatccatt aagtaaatca
    15001 aaaaattcca atatcaatca acctttattt ttatctgaaa aaatattagc aaaaactgga
    15061 aaagcttatt taattgatgc cgacaattta tttttcagtg aaaatatcat gcaaattaaa
    15121 ccaacaccag aaccgattcc tggtgtaaaa ccatttactt taggttcttt aaatagaagc
    15181 aaatccaaat atttaagtat aaaaaacttt cctaacaatt cattagttgc agtttcttat
    15241 gtttacgaca atccatcacc atctaattat ggaaaagatg cagttacgga tgcacgtttt
    15301 gttactatag aaatagctca taatttaatt gctatgcctc aaaatgattt ccaatcaaga
    15361 agtgatgacc caagagttgg attttttatg agtaaaagaa cagaccaaac atcaacttct
    15421 ccaacaccct atttagatgt tattcatcgt tgggatttaa aaaagaaaaa ccctgaacaa
    15481 gcactttctg aaccaattac tccaatcgtt tggtggatag aaaacaccac accaaaagaa
    15541 tttagaccaa ccataaaaga agctgtttta gcttggaata aagcgtttga aaaagctggt
    15601 tttatcaatg ctattgaagt caatgaacaa cctgacaatg ctacttggga agctgacgat
    15661 attagataca atgtattgag atggacttca tcgcctaatc ctgcttttgg aggttatggt
    15721 ccacattttg ccaatccaag aacaggtcaa attttaggtg cagacatcat gctagaattt
    15781 atttatttga ccaatcgagt tatttatgaa aaattatttg atgttgctgg cttaactgaa
    15841 actgaggaaa caactttaca ctccaatcat tcttgtcaat ttgggcaaca aatgcatgaa
    15901 aatattttgg ctggtttaag tgtaatgaat gcaaatgatt tttcagacct tgaacaaaga
    15961 gaatttatga agcaagcttt atttaattta gtacttcacg aaatgggtca tacttttggc
    16021 ttaaatcata actttattgc aagccaagtt ctaagtcctg atgaaatgaa agatattaaa
    16081 atctcgacag aaaaagggct tacatcttct gtgatggatt acacgattcc taatatttct
    16141 tcagataaaa acaaacaagg tttatacttt gatcataaac caggaatgta tgatacttgg
    16201 gctattcaat atggctacac gccttttaaa gatacgatag acgaacaaaa tggtttagca
    16261 aaaatacttt cggaatctac tcaaaaagaa catttattct ttaatgatgg tgatgacatg
    16321 cgatatcctg gaaaaggaat agacccacga gtaatgatta gtgatatgag tagtgatgct
    16381 attgcttatg ctactgaaaa tactgaacag atgatttcta caatgggtaa acttttagat
    16441 aaatacaaaa atgaaaacca atcctattat cctatccgaa atgcatttac tgttttgacg
    16501 agtgcgatgt caagaaattt cggagttgtc agtagatata ttggtggtat ttatattgac
    16561 cgttcattta tgaatcaaaa tagtcctaac aaaccttata cacctgttcc attacaaact
    16621 caaaaagctg caatgaaaat gttgagagat tattgttttg gaccagaagc atttagtaaa
    16681 agcactgaag tttataatta tttgcaatac caaagacgtg gttataatag agatggtaat
    16741 gaagacccaa aattacacga aagattctta aatattcaaa aaggagtcct agaccaatta
    16801 ttaaatacaa atgtgttaca aagaattgaa gatactaaat tatacggcaa tcaatatgac
    16861 ctaaataaaa tgcttacaga tttaaccaat gctattttta ttgaaggctt tagtaatcct
    16921 agtacaatca gacaaaatct gcaaggagaa tacatcaata gattattgaa tattattgat
    16981 gaaaaatcag ggaatactta tagtgtaaag tctgctgttt atggtcaaat agatagagta
    17041 aaaaagcttt tggaagtgag tgcaagcagt ggcagtttgg ctgtaaatgc acaccgccaa
    17101 aacttacttt atttaataaa taataaatta agtatcaaaa actagattaa ttttttttat
    17161 tttatttttt attgttataa ctttaaataa gcaaattaaa ttcttatgaa aagaaatcaa
    17221 tcaaaaatat gtattgtact tatacttttt agcataagtg tattctttag tgcttgtaag
    17281 aaagacaaaa aacaagaaac aatagatgtc tatgtagctg tagatgaatt caatggtact
    17341 tttaacatag ctaaattatg gaaaaatggc attattcaag acttaaatga tggaacaaaa
    17401 tcagcttctg ctagttctat ttttgttcaa ggaagtgatg tttatcttgt aggttttgag
    17461 gaaaactctc ttggaactaa tgttgcaaaa ctatggaaaa atggagttgc ccaaaatcta
    17521 tcagatggag catttgataa ttttgcaaat tcaatttatg tagctaacaa taaaacctat
    17581 attgcgggat atcaggacaa tggaataaaa agagttgcca agatttgggt aaatggagta
    17641 gccacaaacc taacgaatgg gttgaatcat gcatatgcag gttctgtttt tgtaagtgga
    17701 gatgatgtat atgtaggtgg ttatgaagag aacattattg gaatcagcat tgcaaaaatt
    17761 tggaaaaatg gtgtagctca aagtcttact gatggtgtac ataatgctag tgtaaatgct
    17821 atatatgttc aagataacga tgtttatgcg gttggatata atggtgggac tgatatagtt
    17881 tggaaaaatg gtattccaca gaatcttact gatggttctg tttatgctta tgcaaactct
    17941 atttttgtat taaacaatac cgtatatgta gttggtcata aaaataatgg aacacacaat
    18001 gtagcaacct tatggaaaaa tggagttgca caatatatta ctgatggctc aaaaaaatgc
    18061 tcagcaagtt cagtttttgt tctagataat gatatttata tagttggtta tgaggaaaat
    18121 ggaagtaaag atgtggctaa attatggaaa aatggggtag ctcaaaatct aacaaatggc
    18181 acaaatgacg gttcagcaaa ttctgttttt gtaagtaaaa aataatattt agaaagatgc
    18241 tgactactat aagtaagctc gagtttctaa agtcattaaa acaaaaatac ctataaaata
    18301 agctatcata gagccatcat aaaagaacat aatccatgtt ttggatattt tatgggagct
    18361 ataaacagaa attgcaatca atgaaatcaa taataagttg aaaaaaatat gaatttctgt
    18421 actaactcta aaaatgtaca ataggtaact atcaattaga aataatgcta cacacagcat
    18481 ttgacttttt ctagctccaa gtgttgttgc aattgtctta gtttgattag ctacatcacg
    18541 ctcaatatca ttaatatcga aaggcaaagt aataaataat aaataaataa attgaataga
    18601 aaaaagtagt acaacacgtc ttacaaataa atcaggatag gtatttgttt ctaaaattgg
    18661 taataaaacc gcagttatag tccatgttat tgctatcaag aaaattttca caaatggaat
    18721 atctctcaat ctttttttaa ttcctatctt atctaatggc aatgcataaa ctaccgacaa
    18781 aaagcctgaa aatacgattg ctacttgtgt aggaaatttt aaaaataaaa aagtaatccc
    18841 agaaataatt aaagacaaag ccgtagtgat tttagtgatt ttttgatgtt ttatctggta
    18901 ataataaata tcatcttcag tatttttttc attcattttt tgttggtaca acaagcgact
    18961 aaattgatat ataaaccaag ttgaaaaaaa tatatgaacc aacaataata aaatatgatc
    19021 gacttcaata tacaacaaca aagcatttgc cattgtaaaa caaacagcag caatacttat
    19081 aaaaatattt gaatggatta aaaacttaaa aaatgatttc actttatgca aattacaaaa
    19141 agaattattt tgaaagttat attttataaa attttaatgt aagactaata cagtttgatt
    19201 ataatgatgc tctccattta caatttcgac ataatagaca ccttttctaa gctcagaaac
    19261 ttcgatttca gtattataat cattggtagt ggtagtaaaa acagcttgtc ctagttcatt
    19321 gtacaatgca attctatatg gttctttcga acttggcaat tggtctatat ataaataatt
    19381 tgttgctgga ttagggaata cttttatatt tttaaatagt gtattattcg taacttttga
    19441 aataaagtcg caaggaatat tattatttaa gaaactactt cgtgaagagt tgagaacatt
    19501 acgaacatat tccgcttgtt ttttagtaaa caaagccata caagcatcat cggtataatc
    19561 cataaaattt tgatacatac gtccagaagt tgaaggcgaa cattcatcat catatatacc
    19621 tgaaggacaa ccataagtag catcgtattg tttgggtgta tcgcttatgc catcatcgct
    19681 attacaattt cctccatttc cccaaatatg tttcaaaccc aaccaatgtc caatttcgtg
    19741 tgtagttgtt ctacctttat tatagtctgg caataaattc ccaatagtcc caaaaactct
    19801 ataatgtata acaataccat cttttattcc agcattatca ttagggaaag atgcaaatcc
    19861 aagtgtgcca tctgtaaagt tagcaatcca gatatttaga tatttatcag tatcccaagc
    19921 atctaatcca cctttattac tttttttgat ataattttca tctgcatcat ttaagtttcc
    19981 tgaataagaa aatgaagact tagaaacata ttttctttcg ataccagttg ttatttgccc
    20041 ccaagggtct tcagtggcaa tacaaaactc aatttcagta tctcctatcc tatcataaaa
    20101 ttgatgatta ctagagagtt tatccgtatt gagggcacga taatctctat ttagagctga
    20161 aatttgtgat ttaatttgtt catctgaaat attttggctt tctgtattat agagtacatg
    20221 aatcacaaca ggaattcgaa taataccttc agactgagca tatctttgat tattattttt
    20281 taattcattt tgtaatgcaa tctctttttc tacaatttca ggatattctt ttaaaatatt
    20341 ttgataataa ttatcatagc cacatttacg ttgttgggct atacttaatt gatatccgcc
    20401 taaaaggaaa ctaataaaaa tataaacttt agcccagtaa tacatcttta caaaattaat
    20461 ataaataata gtactaaaat ggaaaatatt aaaaattata gctatcttta attttggcaa
    20521 gataattgca ttatttttaa taactaaatt tttaaaaaaa atgggactat ttaattttat
    20581 taaaacagca ggacaaaaag tatttggcaa aaaaccagaa gaaacacaaa cagaagaaga
    20641 aaaacaatta caagcaaaag aacttttagc ttatgtacaa agtttaggtt tagaaattag
    20701 taatttaact gttattgtaa gtggtcaaaa agttacagtt agaggagaag tggcttctca
    20761 agaaattcgt gagaagattc ttttagcatt aggaaatgtt gaaggcgttg cagaagttga
    20821 tgatacaatt tctgtaattg tacctgcacc tgaggctaag ttacacacgg ttgttagtgg
    20881 cgatacgctt tcaaaaattg ctaaaactta ttatggtaac gcaaatgact acaatagaat
    20941 ttttgaagct aacaaaccaa tgctttcaca tccagacaaa atttatgtgg gtcaggtttt
    21001 aagaattcct caagcataat ttatagggat ttaatgaact actaaaaaag ggcaactaaa
    21061 agttgctctt tttttattac actagacagc actaataaaa taatttccat cttccaattt
    21121 gaatcgatag cgaggaattt tgtattgatt accatcagca ctcaatcctg ttttcatatc
    21181 aaacttataa ccatgtagat gacaacctat aactaatctt ttattggcaa aaccatgatg
    21241 caactccgct cctgcatgtg gacaaatatt actaattcca aaaatttctt cttcagtttt
    21301 aacaatacaa atcggttttc cattaaaaac aattttaaat ggtttgtaat ttatagcatc
    21361 aatcattaat tgttgaatat ctgaatctaa taaataccaa ttcataaata agtataattg
    21421 ttcaaagata gcaaaaacaa aaggctaatc aactaaaagt attccaaaaa gttggtatct
    21481 tttccagcta caatacgttt atatctatcg tgtaaaagtc tgccgatggt atcttcccaa
    21541 ggcaatttta attggtgtcc gttaatactt ttaataccag ctacttcggc agcagttcct
    21601 gcaaaaaagg caccatcggc atttaatagt tcttcaactc cgaacaattt ttcttcaaca
    21661 ggaatatcca tttgcttagc caaatcaatt aaagttgctc tagtaattcc ttgaaaaata
    21721 taatttattg gtggaactat caattttcca tctttttcca taaaaaaaga agctccagga
    21781 cccgttgcta cataaccttc cacatctaat aacaaagcat cgtcataacc attttgggtt
    21841 gcctctctag ttgctaaaat ggaattgata taatttccag aagcttttat atccacaggc
    21901 attgtttttg gatgaggtct tctccaagaa gacaccatta cattcaaatg attattggga
    21961 taatattttt tccatttcca acaagtcatt aaaacattag gtttatcagc atcaacagca
    22021 cgcaaaccca tatttttatc cataaaaata aatggtcgaa tataagcttc agataattta
    22081 ttttgctcta ctaattcata ggcaaaattt atcatttcct gaacagaata aggcatttca
    22141 atatgtaatt ttttggcaga ttgcatcaaa cgttcgaaat gtctgcgtac tttataaata
    22201 tgcaatccac tttgtgtttg atatgcacga attccttcga aaacaccata accataatgt
    22261 aaagtctgat taaatggact tattttaaaa tcatttacta atttaaaatc accattgaaa
    22321 aacaaaatgg ttttatcatt atgatacaac atattttaaa gatagaacac aattatagaa
    22381 attcaaaatt aatttatcca aattttgttt aaatcctaaa acaaatttga aattgaagat
    22441 aaattctatt ttttgtttgt atcatcaatt aaatagacaa cattagaaaa ccaaaaatct
    22501 attctattcg catcgacatt tgttttttct atcgttccaa aaataacttt aattttataa
    22561 tttgctaatt gaccttcaat agataataca tctggattat tgggataatt atcaaacaat
    22621 ttgatggcta aaggttttaa attcagcgta tctttattat ttaaaaccat atacaaggca
    22681 ctattttgca atgtttttat ttgaatacta tcttggttaa tattaatatt tttatcctca
    22741 ggatattgtt gcaatatagc atattggtaa tttgcaattt cataataagg ctgtttactt
    22801 gtcaattgaa ggaaattcgc tggataattt ttattagaaa tttgctgaac atttttaaac
    22861 ttagattcga ttggccaata attggactct gcatttttaa attcaacctg atgtttatca
    22921 tctaaaaaac tcaataaaaa agcactttgt ccacgactat ttagaaaatc aaatttgctt
    22981 gaaatttcat agactaaaga atcagtaatc gttttatcaa aatcaatctt cccatttacc
    23041 aataatttat tatccttcaa aatttgttct aaagcatttt tttgacttcg tttagcaaca
    23101 gaaattgcat taaaataagg gaaaactaaa gcaaataacc caaatataaa taaggaaatt
    23161 gggataaatt ttatggtagc atttttaata aaaacaaaat acaaaacaac tgtcgataac
    23221 caaattgcca aaagcaaaac atagtatctc ggctctgtaa aagaataagc taaaattctt
    23281 gtaaaaatag caacaaataa taatatcaat aatggaatta atgtataata aaatagcttt
    23341 ccaaaaacac gcacccaagc cttttcagtt ttatttttga gtggatgtac taaaagtaaa
    23401 gctaaaattc cgacagaaga atatgctaaa atcaaataag aaacccaacc tcttggaagt
    23461 tcccatgtta aaataatttt ggctaaataa acataaagaa taactacata taagaacaat
    23521 aatggaatta atatatattg tgtaaaaaat tgtaaaataa taggatattc atcttctttt
    23581 tcaatcgaac tcaacccact ttcacaaaat gataaaaaaa taaaagtact tccaaaaatt
    23641 aatataaata gaaaaatttt ccaataaaca tcgctatcaa aatgaatttc aaataaatta
    23701 tcaatagcta aaattgccaa ctccgttcct atgattaaaa cgcttacaaa tacaagtgta
    23761 aggaaaaagt ttacaaataa gtttttatta aattgccaaa atgaaaattc taagttttct
    23821 tttttagtaa atgcaataaa tgctaccaaa agatgtgaaa gtatataaat cggaataaga
    23881 ataaatgcat aaaactcatt aaaatctact tcatttttag gtaaaataaa ataaaagcct
    23941 attagaatta gaatgcctaa atattctatc attttggatt gctgcactct ttgtgccaac
    24001 aactttaatg caaagtttaa agaaatgcct aagccagcaa ccaatatcaa tttaaccagc
    24061 aaaaatttta atgaatcatc tttactaaaa ttaaaaattg taatatgaat gatggttatt
    24121 aatgccataa taaatgatga caataatact aatggataaa taccaaatac ttttcgtgct
    24181 tgatttagaa ttataaaaaa cttttctttc atcttttttt taagttatgc tttttaataa
    24241 tttaaaacac tttgcaatct aatatagttt aactaatttt aaagctatga aaaagttaat
    24301 tttagttatg tgtattgcat tggtgcttgg ctatgcttgc aaaaaaaatg atgggacaaa
    24361 cttcgaacca attaagccac ccaaagacac tactattatt attgatacac caaaagtgga
    24421 tacgccaata gttgtagaac ctccttattt taatagaaaa tacaacaaag tttgtttttt
    24481 gatgacgcac aatgcaatga ataatagtga aaaaaaatat cttattccaa atcaaacaca
    24541 cagcataagc aaacaattag caaatggtgt tcgtggtttg atgattgata cttatgatgg
    24601 accaggaggc aatgcattga catatcatgg tgttgaatta atgggcaaac aacctttggt
    24661 tgatgtatta aaagaagttt atttgttttt ggcaaacaac gaaaaagaag tacttactat
    24721 tatttttgaa aacaatggca gcaatgaaca actgattgca gctattgaaa ctgctggatt
    24781 aacttctttt gcttatgtac atggaactga atggaaaaca attaaagaaa tggttaatag
    24841 caatcaacgt ttagtgatgt ttgttgaaag cgacaaaact ccaaaagttg attacttaaa
    24901 acatgcttgg tcatatattt acgacacaaa atacacattc aaaaaagtga gcgaatttga
    24961 ttgtgcaatt aacagaggaa cttatagtga aaacaattta tttttagtca atcattggtt
    25021 atcaaatgga ttaggtttgc ctgaaaagaa actagccaat actgcaaata acccttccgt
    25081 aattcatagt cgcctacaga attgtgaaaa cacacgcaat cattttatta atttcttagg
    25141 tgtagatttc tatgaagtcg gcaacactaa agctatagta gattctataa acagccattt
    25201 tgtagaataa ttttgataaa aagaaaaaaa aagagaatat ttcactttat ttgattactt
    25261 ttgttcaatt aatttttttt ataaatgaac atttacatcg caaacatcaa cttcagaaca
    25321 agtactgagg ctttgcaaca acttttcgag aactatggag aagtaacatc agcaaaaatt
    25381 atcactgaca gagaaaccgg acgttcacgt ggttttggat tcgtagaaat gagcaatgat
    25441 gaagaagctc aaaaagcaat tgacgaactt aatggttcag aatttgacgg aaaagcatta
    25501 tctgttaatg ttgctaaacc taaagagaat aacagtagag gttcttatgg tggtggcaat
    25561 agcaattaca agaaaaaaag ctggtaattt aatttatatt actccaaaat aaaaaactcg
    25621 aaacaaatgt ttcgagtttt tttatgcctt gctcctactt attcgatagc tctgggaatc
    25681 cttcagatgg cattccaaat tttacagcca aattaaaata cattcttgct ttattggtat
    25741 ctctttccga taaataataa taacctaagt tttcataagc agcaaaattc aagctatctt
    25801 tcctaattaa atcaaaatta taattcagtg ttttttcttt aaaaccttgt tcatagtata
    25861 aggtcgaaat attatttatc aatgcctgtt tgtgtctatc agttgcaaat tgataagctc
    25921 ctaaataata attttctgaa tttttataat ctttttttga ataataaaca tctcctaaaa
    25981 attctaaaag actagcattc gtagaatcta tggcaaaagc cattttaaaa tatttttcag
    26041 catttgacaa atcatttttt aaaaataata attgacctaa ttgggtatta ataagcacat
    26101 tattttcata cacttgattc gcatttaatg cacattgtat tcctttattc aaatagctag
    26161 tatcattagt ttctttaaaa ttatcaatat atacatttgt agcaatacgc attccttcat
    26221 aagaattgtg aagtttagga gcatctgttt caactaaagt tgtcaaactc tcccaagctt
    26281 gtgttctaac aaaagaagca gttgtcaata aaactatcat aaaaacaaaa aacccttttg
    26341 tataaatatt ttgtattggt aaaaaatgcg ttaatacaaa aacaaagagt ataataaacc
    26401 aaggcaatgc cataaatgta tatctatcag caacaattcc tgcaacagct gttataaaac
    26461 ctgtagcaaa gaaaataaag gataaaaacc ct
//
LOCUS       DAOQCW010000020        25684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_716_length_25684_cov_5.124585, whole genome shotgun sequence.
ACCESSION   DAOQCW010000020 DAOQCW010000000
VERSION     DAOQCW010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 25684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25684
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_716_length_25684_cov_5.124585"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1724)
                     /locus_tag="PLS16_03140"
     CDS             complement(<1..1724)
                     /locus_tag="PLS16_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNJ60105.1"
                     /translation="METKKINNSMLIPIENMESEHCVLIIERELAKIDGIINYKVELN
                     NARAVLNLEDISIVPNIIQTIKSIGYNVPTIKKTLPVLEMSCASCAASAEDAAKFAEG
                     VIDASVNFATGNLTVEYLPNITDIYKIQKIIQIAGYDLLITEDTDNNQETIEAFHEKK
                     YKTLKRKTIIALVFAIPLMLIGMVFMDMPYADWIMWVLATPLVFWLGKDFYINAWKQT
                     KHKSANMDTLVALSTGIAYVFSVFNLLFPDFWHKRGMHAHVYFEAAGVIIAFILLGRL
                     LEEKAKGNTSTAIKKLMGLQPKVLTIIQKDGSEKQVSIDDVHVGDTILVKPGDKIAVD
                     GIVVKGNSYVDESMLSGEPIPIFKTESEKVFAGTINQKGSFQFSALKVGKDTMLAQII
                     QTVQDAQGSKAPVQKLVDKIAAVFVPVVISIALITLVLWSIFGGENSFVQGLMSAVTV
                     LIIACPCALGLATPTAIMVGVGKGAEQGILIKDAESLETAKKINAVVLDKTGTITEGK
                     PQVSDILWLANNNSKQAILFSIEKKSEHPLADAVVKYFKDENAVEILDFENIVGEGVK
                     AIFENETY"
     gene            complement(1839..2798)
                     /locus_tag="PLS16_03145"
     CDS             complement(1839..2798)
                     /locus_tag="PLS16_03145"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ60106.1"
                     /translation="MSEYSIHKLTKTDFHKLIPLMKDCFGMDVNIKYFEWKFLNNPSG
                     IVEGYYATDEAGETAAYYGVIPETFKVNGSVKVIYQSCDTMTHSNHRRRGLFQKLAIT
                     CYENLRKEDKLFIYGFGGGQSTPGFLKFGWCIVFNMRYYFYPKHFNLFTKLIYKNVFE
                     LSNLKELESLALQSNIDSKVASIKNFEILKWRLANPLQSYKIIAYKENDHFSSYLIYY
                     FENDKIIIFDFFVSNLTAGKNLFNYIKILLKNNNKKGIVSFCQEDSYFSATLKKYSFI
                     NNSLNFGPLRERTPFILYSTEDEQKYMNNAKFWQINSFEHDAL"
     gene            complement(2801..3748)
                     /locus_tag="PLS16_03150"
     CDS             complement(2801..3748)
                     /locus_tag="PLS16_03150"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNJ60107.1"
                     /translation="MRDALLHLIYRLGIPSLLRLKKNGFLTVLCLHRISDERDFFFNP
                     IQVKTFELLIDYLSKYYTIIPFFDAEKKTLKPKLILSFDDGYYDFIEHAMPILKKKGL
                     PANHNIVNDCVNNNATIWTQQLNDIFNHLRDNEITNDKDINQLSNFENNWNLYYLTIF
                     KKFLSMDLEHRVNLLNMIKDKYKISSSYRMMNWDDIKYISKNDVEIGSHTYNHNPLSI
                     INGKQLFQTEILNSIDEINTKIGKSINILSLPNGVANSELSSFIEKSTIKYVLLVDDK
                     INKMPLQNATNYISRIYLMDNPIYETILRTECFHSILKR"
     gene            complement(3818..5170)
                     /gene="radA"
                     /locus_tag="PLS16_03155"
     CDS             complement(3818..5170)
                     /gene="radA"
                     /locus_tag="PLS16_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNJ60108.1"
                     /translation="MNKVKTTYVCQSCGTTSPKWMGKCSNCNEWNTYVEEIVDLSPKG
                     KVKSNINTSSNPQLLADIQNENKARILTSDAELNRVLGGGLVHGSLVLIGGEPGIGKS
                     TLLLQMVLQTPNLKVLYVSGEESEKQIKMRAERIASQNSDIYIYTETSVDSILHQIQL
                     LQPDLVIIDSIQTIETTLLESAPGSVAQIRESTHLLQQYAKKQHIPFLIVGHITKDGT
                     IAGPKLLEHIVDCVLQFEGDRNYNYRILRTIKNRFGSTAELGIYEMQQTGLRCVTNPS
                     ELLITERDEAVSGVAIAITMEGNRALLVEVQALVSSAVYGTPQRSSTGYDLRRLNMIL
                     AVLDKRCGFRFGTKDVFVNIAGGLKIDDPSADLAVVAALLSSYEDVAINNRFCFSGEV
                     GLSGEIRAVNKIENRLSEAEKLGYEKVFISKYNQKTKQLQNTELKLVKSVLDFYKSLF
                     "
     gene            complement(5179..5523)
                     /locus_tag="PLS16_03160"
     CDS             complement(5179..5523)
                     /locus_tag="PLS16_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60109.1"
                     /translation="MNKLLCILLVLFGATSCLKNKEINLSDTRWHTQYKYEGFSFFAE
                     KELVLNKNNTCYEFGNIDTVYGNWVFEDKRLKVDFENKTIIRAIVVHRDSLSGYRLSA
                     TKVSGEWNAKRK"
     gene            complement(5575..6972)
                     /locus_tag="PLS16_03165"
     CDS             complement(5575..6972)
                     /locus_tag="PLS16_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60110.1"
                     /translation="MDKLFTHLTNSWIQDILFLNDIYIGIPFLILVHFLLKKYVLKNS
                     NSPIYQKYLMKAWYFRVFGAVMSALMYQYYYNGGDTTVYFRLSLQLQNMTFTNPGGVW
                     ATFMGSSREDYGMLMNQLFGGASTYLTEEGTRTAILVGYILSFLGLKSYMITSYIISM
                     FALYGCWQIFKIFYELYPHLEKQLAFACLFVPSVFFWGTGLMKDPICLGALGMLHASS
                     YQIFHKRKNIFKNLFLMALSVYIIMTVKVYIILAFAPAIAVWLFARYAETIQNKFVKI
                     ISFPILLAIGGGFGVLILSQMASVANRYSFDNMMRTVQDTQNWLVYSSQQQGGSFYTL
                     GDIEFTTFGILKVFPKAVNVSLFRPYIWEALKPTLFVAAIEAIVTLYLSVMLLFKFKL
                     NIFKFIGKIISNPDILFCFIFSIIFAFAVGFTSFNFGALSRYKLPLIPYYFIFLVLMY
                     DGLSKKVSKKVNNIV"
     gene            complement(6972..7637)
                     /locus_tag="PLS16_03170"
     CDS             complement(6972..7637)
                     /locus_tag="PLS16_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60111.1"
                     /translation="MLAKDTIDYEVRFLYEDTLVYQAIEWCEQCQSKYVFIADDQKQV
                     FGYIASDVLYELEEDNTILELIQPLEEKLLIREDTHIFEILRKIEQVDNSIFPIIENS
                     GLILGFCTANSLLKSYLKDNELLQNGGIITIETDLHDYSLSEIARIVESNDAVILNVV
                     VKQVVAENKLQIILKINKTDLKNIQATFERFEYTVLDIVHESEFDAQMQDRIDNLIKY
                     LEV"
     gene            7780..8568
                     /locus_tag="PLS16_03175"
     CDS             7780..8568
                     /locus_tag="PLS16_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNJ60112.1"
                     /translation="MSKEVLEIVSYNLNGIRSAVSKGLADWIKETGFDVYLFQEIKAD
                     LYSIPVEYFDELGYKHLWFPAQKKGYSGVGVIYKNTIDVNQIKQGIAIPKYDFEGRVI
                     QFNIKDITFLNTYFPSGSSGDERQAVKMEFLNDYFDYIHQLKNTNPKIILSGDYNICH
                     TEIDIHDPKGNKNSSGFLPEERAWMTKFFDSGFIDTFRFINKDLKDQYSWWSYRAGAR
                     AKNKGWRIDYQSCTENMQEQILDAKILMDVKHSDHCPIYLKIKI"
     gene            8565..10298
                     /locus_tag="PLS16_03180"
     CDS             8565..10298
                     /locus_tag="PLS16_03180"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNJ60113.1"
                     /translation="MKYFFIICSFVLITSCIGKKKEFVYTPTDGGRYYGGTFRYNEEE
                     YFKSLFPLNITEVTGHRIAEQIYEGLVKFDDSTLAVIPCLAKNWDISPDGLVYTFHLR
                     DNVYFHDDACFKDGKGRKVTASDFKYCLDKVAFNNPAENQGFWIFKDIVKGANEYYTA
                     TETNKQDTSGISGITAINDTTLRIELTEPYAVFLARLGLTFAKVYPKEAFEMYGSDMR
                     NHCVGTGPFYIKKLLDNQVVFMTKNKNYWAKDEFGNPLPYLDNIKVTFIHEKKMELQE
                     FIGGKSDLIYKFPLEMIDEIIDIDRNLKPQFQKFQLQYLPSMSLQYYGFLHIGKTFNN
                     KYLRQAFCYAVDREKLCKYTLKNSGFPAKYGVVPPGTGTYNSSLIHGFTYQPEKARQL
                     LAQAGFPNGKNFPKVTLQLNSGGARNTQVAEAIKKMIEETLNIKIDLLLIPWAQHSEA
                     VEAAKADFYRMGWIADYPDAENFLNLFHSKYVPSDPTEKTYINSFRYKSKAFDNYFDA
                     ALRTTDMNERNALYNKADQQVIDDAVVMPTYYDIDFRLLQPNIRNFPQNSMEYRDMAE
                     VYMLPPTKNKK"
     gene            10305..>10822
                     /locus_tag="PLS16_03185"
     CDS             10305..>10822
                     /locus_tag="PLS16_03185"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNJ60114.1"
                     /translation="MKIFCIGLNYREHIDEMKHFSFPDNPVIFMKPPTALLKDKQPLY
                     YPDFSNDVHYEGEIVLKISKNGKKIEEKFAHKYYDSFSLGFDFTARDIQADLKKKGWP
                     WEIAKSFDQSACIGEFIPLDKEKNIDFTIKKNNILVQHGNTEMLIFSFDKIVSYVSQF
                     FTLQQGDLIYTGT"
     assembly_gap    10823..10922
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10923..12143
                     /locus_tag="PLS16_03190"
     CDS             <10923..12143
                     /locus_tag="PLS16_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60115.1"
                     /translation="VCFLSSKAAFILIPMDNTQKNHLKAYGIAYLALTKDIPLDWLLN
                     YRGGSFALPQNSILEKECLIRNVSYEVIPDAKMSSILTEISNPEVNMDAVRLEKAPKV
                     AVYSPKSKLPWDDAVTLALTYAEIPYDVIYDEEILEGKLHLYDWLHLHHEDFTGQYGK
                     FWSAYQFADWYKEQVAEYEMRAKKLGFNKVSDEKRAVALKIKDFVIGGGFLFAMCSAT
                     DSYDIALATEGIDICDVMFDGDPMDPHAQDKIDYHKGFAFKDYELSTNPYEYEYSEID
                     ALPLGRDQSTDYFSLFDFSAKWDQVPSMLTQNHEKILKGFMGQTTAFRNPFVKTEVLV
                     LGENKSLDEAKYIHGTQGKGTWTFYGGHDPEDYQHRVGDPPTDLNLHPNSPGYRLILN
                     NILFPAAKKKKQKT"
     gene            12157..12822
                     /locus_tag="PLS16_03195"
     CDS             12157..12822
                     /locus_tag="PLS16_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60116.1"
                     /translation="MNILKNFISICTVILLSYHAYGQEIKQTEYSFDINKTIDGTDYF
                     YYFNKGKNDTFINLKIMTFNIDNTVKTLCKEVSVLPEFYNIATQKIKIDLWNMNVGNP
                     LEYEDVLNNQINVFAKDTSWQNYYKVFSNQASKNKNIQLDYNLISKIMTDQKVYGCIE
                     LELNKIGYTISQVGIEKVGFLDLKSYPAIKKRLSGKLPYSDSQLKTIPIPYIVYIGIN
                     RIS"
     gene            12826..13221
                     /locus_tag="PLS16_03200"
     CDS             12826..13221
                     /locus_tag="PLS16_03200"
                     /inference="COORDINATES: protein motif:HMM:NF022703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3060 domain-containing protein"
                     /protein_id="HNJ60117.1"
                     /translation="MRKLGLLFFTFFALIVIAGPPEIPEPKVKNATILDAQTIIINLN
                     EDDNIQENANHQDILISGISNKINIKGNCKKVLISGMDNEIILDTVQEIILKGNRNTL
                     TWNYSKLPDNKPIITDKGFQNKIEQKTIE"
     gene            13221..13913
                     /locus_tag="PLS16_03205"
     CDS             13221..13913
                     /locus_tag="PLS16_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNJ60118.1"
                     /translation="MNTLYLIPTLLSENSASSIPEYITPIIEKCDVFIVENLKTARRF
                     IKLILKEKDIDVCTFIEMDKHKNYSIDEQELYLFKDTNIGLMSEAGAPCIADPGNKIV
                     LLAHELNWKIVPLVGPSSILLALIGSGFNGQSFSFNGYLPFDAKERKSKILEMERLAN
                     KNITQIFMDTPYRNVKLLEEIIHCCSKNTLLSISCNLTAQDEFTYTKTIGTWKNTKID
                     IHKKPCIFVLGK"
     gene            13922..14506
                     /locus_tag="PLS16_03210"
     CDS             13922..14506
                     /locus_tag="PLS16_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HNJ60119.1"
                     /translation="MILIDDVLISDDIIEKQFVCNIDKCKGACCVEGDSGAPVADDER
                     KILEEIYSKIKAYLTDEGIKAIEAQGTWVKESDDEYEPYATPLINGGACAYVNFDEKG
                     AAYCGIEKAYNDGIINFKKPISCHLYPIRLSKMETVIAMNYDVWDICKDACILGKKLK
                     MPLYKFLKEPIIRKFGEEFYEVLEQVANNEIEPE"
     gene            14539..16104
                     /locus_tag="PLS16_03215"
     CDS             14539..16104
                     /locus_tag="PLS16_03215"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNJ60120.1"
                     /translation="MNDYLLKIKLLAPTLLITTLSSIVGLAIFRWVFTIHYNIIDIPE
                     EIWGYWIPVSLSGIIIILCLKNKFKIFINPKLSDETKPRFALLFLIWIYFSILLIISQ
                     AYLETSSYSLIQLNNIDEIEQSDNNKYFKIDNFSIANTYGGSHTHISYSGKYNQQQYF
                     SIYFVIPIFNKNKTNHPIVPKYWYSKKFISKSFSNKLSNIERNKQISKFFIESAKEMD
                     SLNYYNFEYFEKVQNSFELENYHNAIKSRIGQTTRNNFIVLKPIKEKFEHRNGKKIEW
                     FIGIYILGFGLFLLFIKFAKHDKHLLNQYIKTSTYKQDNIDKILRYFIPSKTHFISSI
                     FLDITSIVFVIFFFLRNNIFHTNKNELLEWGALQKEELYNGELYRLITNLFIHSSYIH
                     FLVDIFGWIIIAGFVEKIIGYKKLWISSLIIGVLSNLISITLESNILCIGSTNIIMGI
                     IGIQLIFLSKQINTPPIKSGLFLILICTFLSIILISSLTGHTNISANISGFIVGISIG
                     FILFNNYEWKKLF"
     gene            complement(16157..17272)
                     /locus_tag="PLS16_03220"
     CDS             complement(16157..17272)
                     /locus_tag="PLS16_03220"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60121.1"
                     /translation="MQFHQVIGHEEIKKHLIGLVQSNNIPHAQLFLSSSGVGSLPLTL
                     AFAQYLVCENKHAEDSCGVCGECIKSSKMIHPDIHYTYPVVPKKSNKPSLSTDYIVEW
                     RKIILNNAYIDESKWLQNIEAENRQGNISADEARTIIRDLSLKSYESRFKIQIIWLAE
                     ALGKEGNILLKLLEEPPEDTILLLISENPDQILPTIISRCQLIKLHQLSESEIEEALV
                     KNGIPSTKAQKIAFLSNGSISEALEIAEKEIDDITYTLVDWMKLSIHYDILALVKWVD
                     DLSKMGREKIKTFFEYTEHVLRETLLTKYQDAYIPKLNDEELKIAQALKKYLKNHEQI
                     HEFIQLLDEKYYEIERNVNGKIVLLSASLKLHSIIKQ"
     gene            17421..18920
                     /gene="gltX"
                     /locus_tag="PLS16_03225"
     CDS             17421..18920
                     /gene="gltX"
                     /locus_tag="PLS16_03225"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNJ60122.1"
                     /translation="MTDIRVRFAPSPTGPLHLGGVRTALYNYLFAKKNKGTFILRIED
                     TDQNRFVPGAEKYIMDSLAWCGIKIDEGINEGGDFGPYKQSERKELYKQYADALIQSG
                     QAYYAFDTAEELEQLRKENPNFAYNSKVRGQLNNSLNLDNDSIQNKLNSGIPYVIRIK
                     VPAETEISFNDIVRGAVSFHSSQIDDKVMFKSDGMPTYHLANVVDDHLMKISHVIRGE
                     EWLPSTPIHVLLYEFLGWSATMPHFAHLPLILKPDGNGKLSKRDGDRLGFPVFPLNWT
                     NPESGEESQGFKERGFLPEAFVNFLAFLGWNPGTDQEILSMQELIELFNIEKLHKSGA
                     KFDFEKAKWYNASYIKLLESSTIASWLQAQNQFQNIDLDYLTKAIELVKERLIFLQDI
                     QHFNYLFDIPNNYDETNLAKLGTIKSNFNIKQFENTINNIATFNSSSIEASIKEFAEN
                     HQIKVGEMMKFLRISLVGELSGPAIPDLIVLLGKVNTLNRIELGLAKCQ"
     gene            19050..20225
                     /locus_tag="PLS16_03230"
     CDS             19050..20225
                     /locus_tag="PLS16_03230"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNJ60123.1"
                     /translation="MREVFIVSFARTPIGSLGGVLASVNTVELGKIAVQAAMERAKVN
                     GDMVDEVILGNILQANVGQAPAQQVSIAAGINTKTPCTTVNKVCASGMKAIMLGAQSI
                     QLGDNDVVVVGGMESMSNAPHYLPSGRTGIRYGNGEIIDAIVRDGLQDPYKKYMMGNA
                     GEVCAKHYSFSREDQDNYAIASYERAAEAYKNGYFNEEIIPVTIATRKGNITISEDEE
                     YKKFDKEKVGTLKPAFEKEGTITAINASKINDGASAMVLMSGEKVKELGLTPLAKIVA
                     YADASQEPEWFTTTPSKAIPKALAKANIDIKSVDYFEINEAFSVVALANMKEMSIAHE
                     KVNAFGGAVALGHPIGNSGCRIVGTLISILKHKNGKIGAAGICNGGGGASAIIIEKV"
     gene            complement(20284..21012)
                     /gene="lptB"
                     /locus_tag="PLS16_03235"
     CDS             complement(20284..21012)
                     /gene="lptB"
                     /locus_tag="PLS16_03235"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNJ60124.1"
                     /translation="MVLRAENLFKTYGSRNVVDNVSIEVKQGEVVGLLGPNGAGKTTT
                     FYMTVGLVTPNKGNVFLDNHNISKVPMYKRAKYGVGYLPQENSVFRTLSVEDNIKAIL
                     EFSDLNKQQQAEKLEALLDEFGLQKIRKSKGKLLSGGERRRTEIARALASDPKFILLD
                     EPFAGIDPIAVEDIQHIVEKLKEKNIGILITDHNVHETLSITDRAYLLFEGKIIKQGT
                     AEDLANDEMVRKVYLGQSFELRKK"
     gene            complement(21016..>21992)
                     /locus_tag="PLS16_03240"
     CDS             complement(21016..>21992)
                     /locus_tag="PLS16_03240"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNJ60125.1"
                     /translation="NRILAKFGVDLVNKKPRPGIQALINELKLNRKLEITDLVFIIGP
                     RINAAGRIAHGSEAVELLVAKDLETAITKIKKVQENNDTRKEFDKDTTEEAFTLIEQS
                     ELLIQKKSTVLFRPHWHKGVIGIVASRLIDKYHRPTIILTESNGKGVGSGRSVPGFDL
                     YEAIDQCSEHLIQFGGHKYAAGLTIELDKIDVFTEMFESVVQHNITEELLIAPIDIDA
                     EIEFSDITDKFYNIIEQMAPFGPENMRPTFVTKDVYDTGFSKVVKEAHLKINLLKDGQ
                     NPVNGIGFGMADLYKIMENKDTFDVCYQLYANEWNNQIKIELRLKDIE"
     assembly_gap    21993..22092
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22093..22868)
                     /locus_tag="PLS16_03245"
     CDS             complement(<22093..22868)
                     /locus_tag="PLS16_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNJ60126.1"
                     /translation="MSEQKRWIFLDADEQKVRHLQDVLKIHPVLCRLLVLRGISTYDE
                     AKQFFRPSLKDLHNPFLMKDMDKAIDRIEKAIQHNENVLIYGDYDVDGTTSVALVYSF
                     FSKIYPNLITYIPDRYSEGYGISIQGIDFAKQHQVSLIIVLDCGIKAHEKINYAKSLG
                     IDFIICDHHLPAETIPDAVAVLDPKRPDCNYPYKELSGCGIGFKLIQAYAEKNNINKE
                     QIYEELDLVATSIASDLVHVTGENRILAKFGVDLVNKKPRP"
     gene            22982..23293
                     /locus_tag="PLS16_03250"
     CDS             22982..23293
                     /locus_tag="PLS16_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60127.1"
                     /translation="MKKLIYHAVGTTHLIQEKIKELLEDLIQNQHYTEEEGKRIVDDF
                     FGDIKSNIETVKFSMEVKLDELFKKLNIDSFTTLKSNINQVIKDIEDNPFLPTIINHK
                     K"
     gene            23309..24361
                     /gene="aroB"
                     /locus_tag="PLS16_03255"
     CDS             23309..24361
                     /gene="aroB"
                     /locus_tag="PLS16_03255"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNJ60128.1"
                     /translation="MKTIELHNYNIIFDDTFKSLERFLDSKNYSKKIVLVDENTKEHC
                     LPIFSKNIKSDVVIEIQSGEANKNIDTCELIWKSLIDYTIDRNAVFINLGGGVIGDMG
                     GFAASCYKRGIDFIQIPTTVLSQVDSSIGGKLGIDFKYGKNLIGLFQNPKLVYINKEF
                     LKTLPKRQYINGFAEIFKHALIQDAVHWQTLKNIDIYNENIDEVLYQSLLVKKQVVEA
                     DPFEKGLRKILNFGHTIGHAIEAYSLENEANPLLHGEAIAIGMICEAYLSYKKTGLSE
                     TELREIHQTLLSYFPKYNISKFPTDKIIQSMMMDKKNKGKNIQTALLNKIGNCVFDIN
                     IIEKEIADSLDFYKFS"
     gene            24479..25480
                     /gene="asnA"
                     /locus_tag="PLS16_03260"
     CDS             24479..25480
                     /gene="asnA"
                     /locus_tag="PLS16_03260"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HNJ60129.1"
                     /translation="MNEILQREAAITIVKDTFTRFLCDYLHLTKISSPVAVLDGTGIN
                     DDLNGIERPVSFPIKALNEQRAVVVHSLAKWKRLRLKELEVEAGRGILTDMRALRPDE
                     DYSPIHSIYVDQWDWEKHIKPENRNIDYLKDTVKRIYSAIKETEKAIQQYYPNEEPVL
                     PEEIFFIHSEELLQKYPGLTPKERENAITKEHGAVFIMGIGGALSNGEAHDGRAADYD
                     DWSTLNQDGFNGLNGDIILWHPKLQSAFEVSSMGIRVDKSALEKQLKIKGMEDKAKLS
                     FHSMLLNGELTESIGGGIGQSRICMFMLNKSHIGEVQVSIWPEEERQKLQAQGITLL"
     gene            complement(25472..>25684)
                     /locus_tag="PLS16_03265"
     CDS             complement(25472..>25684)
                     /locus_tag="PLS16_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HNJ60130.1"
                     /translation="VNITGKTGFVLPVGDVDGMVEKCLYLLQNEEILEQFRRNAYQNA
                     LQFKIENIVPMYEDFYNEIKLKGCLQ"
BASE COUNT         9233 a         3947 c         3628 g         8676 t
ORIGIN      
        1 aaataagttt cattttcaaa aattgctttt actccttctc caactatatt ttcaaaatct
       61 aaaatttcta ctgcattttc atctttaaaa tatttaacaa cagcatctgc caatgggtgt
      121 tctgattttt tttcaatact gaataaaatt gcttgctttg aattattatt tgccaaccac
      181 aatatatcag aaacttgtgg tttaccttcg gtaattgtac ctgttttatc taataccact
      241 gcattaattt ttttagcagt ttctaagctt tcagcatctt taattaaaat tccttgttca
      301 gcaccttttc caacgcccac cataattgca gttggagtag ctaagcctaa agcacaagga
      361 caggcaataa ttaaaaccgt aacggcagac atcaaacctt gaacaaaact attttctcca
      421 ccaaaaatcg accaaagtac taaagtaatt agtgcaatac tgataactac aggcacaaaa
      481 actgctgcaa ttttatctac taatttttga acaggagctt tacttccttg agcatcttga
      541 acggtttgaa taatctgagc caacattgta tctttgccca ctttcaaggc actaaattga
      601 aaacttcctt tctgattgat agttcctgca aaaacttttt ctgactccgt tttaaaaata
      661 ggaattggct caccactcaa catactttca tcgacataag aatttccttt tacaacgatg
      721 ccatcaaccg caattttatc accaggttta actaaaatag tatctccaac atggacatca
      781 tcaatagaaa cttgtttttc acttccatct ttttgtataa ttgtaagtac ttttggctgt
      841 aagcccatta attttttgat tgctgtagaa gtatttcctt ttgctttttc ttctagcaat
      901 ctacctaata aaataaacgc tataattaca cctgctgctt caaaatatac gtgtgcatgc
      961 attcctcttt tatgccaaaa atcaggaaat agcaaattaa atacactaaa aacataagca
     1021 attccagtac tcaatgccac taaagtatcc atattagcag acttatgttt cgtttgtttc
     1081 caagcattga tataaaaatc tttaccaagc caaaaaacta aaggcgtagc caaaacccac
     1141 attatccaat ctgcataagg catatccata aataccatac caatcaacat taatggaatt
     1201 gcaaaaacta aggcgataat tgtttttcgt tttaatgttt tatatttttt ttcatgaaat
     1261 gcttctatcg tttcttgatt attatcggta tcttcagtaa tcaataagtc ataaccagca
     1321 atttgtatga ttttctggat tttataaata tccgtaatat tcggtaaata ttctacagtc
     1381 agatttccag ttgcaaaatt tacagatgca tcaataacac cttccgcaaa cttagcagca
     1441 tcttcagcac tagcagcaca cgacgcacaa ctcatttcta acactggtaa cgtttttttg
     1501 atagtaggaa cattatatcc aatgcttttt atagtctgaa taatatttgg cactatcgat
     1561 atgtcttcaa gattcaaaac agctctcgca ttattcaatt ctactttata atttataatt
     1621 ccatcaatct ttgctaattc cctttcaata attaacacac aatgttcgct ttccatattt
     1681 tctattggaa tgagcattga attattaatt ttctttgtct ccataatcta ctttttatga
     1741 gtacaaaatt agacatttca attccaaacc ttattacaat actttttatt tgttttgtaa
     1801 gatttactga aaatgaattt aaaaaattag ttaacaaatt ataatgcatc atgttcaaaa
     1861 gaattaatct gccaaaattt ggcattattc atatatttct gttcatcttc tgtagaatat
     1921 agaataaaag gtgttctttc tcttaatgga ccgaaattca gagaattatt tataaaacta
     1981 tattttttta aagttgctga aaaataagag tcctcttgac agaacgaaac tattcctttc
     2041 ttattattat tctttagaag aatctttata tagttaaaaa gattttttcc tgcagttaaa
     2101 tttgaaacaa aaaaatcaaa aataataatt ttatcatttt caaaataata aatcaagtaa
     2161 cttgaaaaat ggtcattttc tttgtaagca ataattttat aactttgtaa aggattggct
     2221 agtctccatt ttaatatttc aaaatttttg attgaagcaa ctttagaatc aatattactt
     2281 tgtagagcaa gagattccaa ctctttcaaa ttacttaatt caaacacatt tttataaatt
     2341 agtttagtaa agagattaaa atgcttagga taaaaataat atctcatatt aaaaactata
     2401 caccatccga actttaaaaa tccaggagtg gattgtccgc ctccaaatcc ataaataaat
     2461 aatttatctt cctttcttag attttcataa caagtaatcg ccaatttttg aaacaaacct
     2521 cttctgcgat gattcgaatg tgtcattgta tcacatgatt gataaattac tttcacacta
     2581 ccattcactt tgaaagtttc agggattaca ccatagtatg cagctgtttc tcctgcttca
     2641 tcggtcgcat aataaccctc tacaatgcct gatggattat tcaaaaattt ccattcaaag
     2701 tattttatgt ttacatccat cccaaagcag tctttcatta aaggaattaa tttatgaaaa
     2761 tcagttttag ttaatttatg tattgaatat tcgctcatta ttatcttttt aatattgaat
     2821 gaaaacattc tgttcttaaa atagtttcat atataggatt atccattaaa tatatcctac
     2881 ttatataatt tgttgcattc tgtaatggca ttttattaat tttatcatct actagaagta
     2941 catactttat agttgatttc tcaataaaag aggataactc tgaattagca accccatttg
     3001 gtaaggataa aatattaatt gattttccta ttttagtatt tatttcatca atagaattta
     3061 agatttctgt ttgaaataat tgctttccat ttataatact taatgggtta tgattataag
     3121 tatggcttcc aatctcaacg tcatttttag atatatattt aatatcatcc caattcatca
     3181 ttctataact tgaactaatt ttgtacttat ccttaatcat atttagtaag ttcactctat
     3241 gctctaaatc catacttaaa aacttcttaa atatggtaag ataatataaa ttccaattat
     3301 tttcaaaatt tgaaagctga tttatatcct tatcattagt aatttcatta tccctcaaat
     3361 ggttgaaaat atcatttaat tgttgagtcc agatggtcgc attattattt acacaatcat
     3421 taacaatatt atggttggct ggcaaacctt tcttttttaa aataggcata gcatgttcta
     3481 taaaatcata gtatccatca tcaaaactca atataagttt tggttttaaa gtcttctttt
     3541 cagcatcaaa aaaaggtatt attgtatagt attttgaaag ataatcaata agtaattcaa
     3601 atgtcttaac ttgaattgga ttaaaaaaaa agtctctttc atcagaaatt ctgtgtaaac
     3661 aaagcactgt aagaaagcca ttctttttta atcttaataa acttgggata cccaatctat
     3721 aaattaaatg taataatgca tctctcatat ttaatttatt attcttttac aatcatgctt
     3781 taagtatatt aatttaatca atacaatata ccactattta aaatagactt ttataaaaat
     3841 ccaatacaga ttttactaat ttcaattccg tattttggag ttgttttgtt ttctggttat
     3901 attttgaaat aaataccttt tcataaccta gtttttcagc ttcgcttaat cggttttcaa
     3961 ttttatttac agcccgtatt tctccactca atccaacttc tccactaaaa caaaaacgat
     4021 tattaattgc aacatcttca taagatgaaa gtaaagcagc gacaacagct aaatctgcac
     4081 ttggatcatc aattttcaat ccaccagcaa tgtttacaaa aacatctttt gtgccaaacc
     4141 taaagccaca acgcttatct aaaactgcta aaatcatatt tagtcttcgt aaatcataac
     4201 cagtactact tcgttgaggt gtgccataaa cagcagaaga aactaatgct tgaacttcta
     4261 ccaacaaagc tctattgcct tccatagtaa ttgcaattgc gacaccactc acggcttcat
     4321 ctcgttcagt aattaatagt tccgatggat ttgtaacgca acgcaatccg gtttgttgca
     4381 tttcgtaaat acctaattca gcagtagagc caaaacgatt tttaattgtt cttaatattc
     4441 tgtaattata atttctatca ccttcaaact gcaaaacaca atccacaata tgttctaata
     4501 atttagggcc agcaattgtt ccatctttcg taatatgtcc gacaattaaa aaaggaatat
     4561 gttgtttttt tgcatattgt tgtaacaaat gagtagattc tctaatttga gcaacacttc
     4621 ctggagctga ctctaacaaa gttgtttcaa tagtttggat agaatcaata attactaggt
     4681 ctggctgtaa caattgaatt tgatgcaaaa tgctatccac cgaagtttca gtataaatat
     4741 aaatgtcaga attttgagaa gcaattctct cagcacgcat tttaatttgc ttctcactct
     4801 cctcgcctga tacataaagc acttttaaat ttggtgtttg caataccatt tgtaataaca
     4861 aggtagattt tccaataccc ggttcgcctc caattaaaac caatgaacca tgtactaagc
     4921 caccacctaa aacacgattt aactccgcat ctgaagttag aattcttgct ttattttcat
     4981 tttgaatatc tgctaataat tgaggattgg aacttgtatt tatattagat tttactttcc
     5041 cctttggaga taaatcaaca atttcttcta cataagtatt ccattcatta caattactac
     5101 attttcccat ccattttggc gatgtagtac cacaagactg gcaaacataa gttgttttta
     5161 ctttattcaa tttttgtatt atttacgttt agcattccat tctccagaaa ctttagtggc
     5221 actcaatcga taaccactta gactatctct atgtacaaca atcgctctga taatagtttt
     5281 attttcaaaa tctactttta atcttttatc ttcaaatacc caatttccat aaactgtatc
     5341 tatatttcca aattcatagc aagtattatt tttatttaaa actaattctt tttctgcaaa
     5401 aaaagagaag ccttcatatt tatattgagt atgccatctt gtatcagata aattaatttc
     5461 tttatttttt aaacaagagg tagctccaaa taaaactaat aatatacaga gtagtttatt
     5521 catataattt tttatcaaga taatcaaaaa atattaatct atatactaat attctcatac
     5581 aatattattt acctttttac ttactttctt tgataagcca tcatacatca gtactaaaaa
     5641 gataaaataa taaggaatca agggtaattt ataccttgat aatgccccaa aattaaaact
     5701 tgtgaatcca acagcaaagg caaatattat agagaaaata aaacaaaata aaatatctgg
     5761 attagaaata atcttaccaa taaatttaaa aatatttagt ttaaatttaa acaagagcat
     5821 taccgaaaga tataaagtta ctattgcttc tatagctgct acaaataaag ttggtttcaa
     5881 ggcttcccaa atataaggtc gaaataaaga tacatttact gcttttggaa atacttttaa
     5941 aataccaaaa gtcgtgaatt caatatctcc taatgtatag aaagagccgc cttgttgttg
     6001 agaagaatat actaaccagt tttgcgtatc ttgtactgtt cgcatcatat tgtcaaaact
     6061 ataacgatta gcaacagatg ccatttggct caatataaga acaccaaatc ctccacctat
     6121 tgctaataaa attggaaacg aaataatttt tacaaattta ttttggatag tctctgcata
     6181 ccttgcaaac aaccaaacag cgattgcagg agcaaaagcc aaaataatat acactttaac
     6241 tgtcataata atatagactg aaagtgccat tagaaataaa ttcttgaata tatttttccg
     6301 tttatggaaa atttgatatg atgatgcgtg taacattcct aaggcaccta aacaaattgg
     6361 gtctttcatt aaaccagttc cccaaaaaaa cacagatggc acaaacaaac aagcaaaagc
     6421 caattgcttt tctaaatgtg ggtataattc gtaaaatatt ttaaaaattt gccaacaacc
     6481 ataaagggca aacatactga taatataaga tgtaatcata tacgatttca accctagaaa
     6541 acttaaaata taacccacca gaattgctgt cctagtccct tcttctgtta aataagtact
     6601 tgctcctcca aataattgat tcattaacat tccatagtct tctctggaac ttcccataaa
     6661 ggttgcccaa acaccaccag gatttgtaaa ggtcatgttt tgtaattgta aagacaatct
     6721 aaaatatacc gttgtgtcgc caccgttata ataatactga tacattaaag cagacatcac
     6781 agctccaaac actctaaaat accatgcttt cattaaatat ttctgataaa tcggtgagtt
     6841 actatttttc agaacatact tttttagaag aaaatgtact aaaattaaaa atggaatacc
     6901 aatatagata tcgtttagaa ataaaatatc ttgaatccat gaattggtta aatgggtaaa
     6961 taatttatcc attagacttc taaatatttt ataagattat ctatacgatc ttgcatttga
     7021 gcatcaaatt cagactcatg gacaatatct aaaactgtat attcaaatcg ctcaaaagtt
     7081 gcttgtatgt ttttcaaatc tgttttatta attttaagga ttatttgaag cttattttca
     7141 gctactactt gctttacaac cacatttaaa atcacagcat cattactttc tactattcgg
     7201 gcaatttctg acaatgaata atcgtgcaaa tctgtctcaa tagtaataat accaccattt
     7261 tgtaataatt cattatcttt cagataagac ttcaaaaggc tattcgcagt acaaaatcct
     7321 aaaatcaagc ctgaattttc tataattgga aatatagaat tatcaacttg ttcaattttt
     7381 ctaagtattt caaaaatatg ggtatcctct ctaattagca atttttcttc taaaggttgt
     7441 atcaattcta aaattgtatt atcctcctct aattcatata aaacatcgct agctatatag
     7501 ccaaaaactt gcttttggtc atctgcaata aaaacatatt tactttgaca ctgttcacac
     7561 cattcaatcg cttgatagac aagtgtatct tcatataaaa atcttacttc gtaatctatt
     7621 gtatccttag ctaacataat caatttaaaa gtacaatcta tataccaaaa atcaaaatat
     7681 agtacgcaca aaaatgaaaa agcaatgcaa aatagcatat tttatagaaa tctattatct
     7741 tgcataatgc ttatgcatat ttctattgga taaaaaatta tgagtaaaga agtattagaa
     7801 atagtatcct ataacctaaa tggtattcgc tcagcggtta gcaaaggatt agcagattgg
     7861 attaaagaaa caggatttga tgtttatcta tttcaagaaa ttaaagctga tttatattct
     7921 attcctgtcg aatattttga tgaattgggt tataaacatt tatggtttcc tgcacaaaaa
     7981 aaaggttata gtggcgttgg tgttatatat aaaaacacga ttgatgtcaa tcagattaaa
     8041 caaggtatag ctattccaaa atatgatttt gaaggcagag taattcaatt caatatcaag
     8101 gatataacat ttctaaacac ctatttccct tctggctcaa gtggtgatga aagacaggct
     8161 gtaaagatgg aattcttaaa tgattatttc gattatatcc atcaattaaa aaatactaat
     8221 cctaaaataa ttctttctgg agattataat atttgtcata cagaaattga tatacatgac
     8281 cctaaaggaa acaaaaattc atctggcttt ttacctgaag aaagagcatg gatgacaaag
     8341 ttttttgact caggatttat tgatactttc aggtttatca acaaagattt aaaagaccag
     8401 tacagttggt ggagctatcg agcaggtgct agagccaaaa acaaaggctg gcgtattgac
     8461 taccaaagtt gcactgaaaa tatgcaagaa caaatattag atgcaaaaat tttaatggat
     8521 gtaaaacatt ctgaccattg tcctatttat ttaaaaataa aaatatgaag tactttttta
     8581 ttatatgttc atttgtattg ataacttcat gtattggcaa gaaaaaggaa ttcgtatata
     8641 cgccaactga tggtggtcga tattatggtg gtacatttag gtacaatgaa gaagagtact
     8701 tcaaatcatt attcccgctc aatattactg aagttactgg tcatagaatt gcagaacaaa
     8761 tttatgaagg attggtaaag tttgatgata gtacattagc agttattcca tgcttagcta
     8821 agaattggga tatttctcct gatggtttag tttatacttt tcacctccga gataatgttt
     8881 attttcatga tgatgcttgt tttaaagatg gaaaaggacg aaaagttact gcatcagatt
     8941 ttaagtattg tttggacaaa gttgccttta ataatccagc agaaaatcaa gggttttgga
     9001 tatttaaaga tattgtgaaa ggagccaatg aatattacac agctacagaa actaacaaac
     9061 aggatacttc aggaattagt ggtattactg caattaatga tacaacttta agaatagaac
     9121 taactgaacc ttatgcagta ttcttagcaa gattaggttt aacttttgca aaagtttatc
     9181 caaaagaagc ttttgaaatg tatggttcag atatgagaaa tcattgtgtc ggaactggtc
     9241 ctttttatat caaaaaatta ttagacaacc aagtagtttt tatgacaaaa aataaaaatt
     9301 attgggctaa agatgaattt gggaatccac ttccctattt ggacaatata aaagttacat
     9361 ttattcacga aaagaaaatg gaattgcaag aatttatcgg cggaaaatct gatttgattt
     9421 ataaatttcc attagaaatg atagatgaaa ttattgatat agatagaaac ctaaaacctc
     9481 aatttcagaa attccaattg caatatctac cttcgatgag tttacaatat tatggatttt
     9541 tacatatagg caaaactttc aataataaat atttaagaca agcattctgt tatgcagtag
     9601 atagagaaaa actctgcaaa tacaccttga aaaattctgg atttcctgca aaatatggag
     9661 ttgttccgcc aggaactgga acttataatt cttcattaat acatggattt acttatcaac
     9721 ctgaaaaagc aagacaactt ttagcacaag cgggttttcc aaatgggaaa aattttccaa
     9781 aggttacttt acagttgaat agtggtggtg ctagaaatac tcaagttgct gaagctatca
     9841 aaaaaatgat tgaagaaacc ttaaatataa aaattgattt attgctaatt ccttgggctc
     9901 aacattcaga agctgttgaa gctgccaaag ctgattttta tagaatgggt tggattgcag
     9961 actacccaga tgctgagaat tttttaaatt tatttcatag caaatatgtt ccctcagacc
    10021 caactgagaa aacctatatc aattcatttc gctacaagag taaggctttt gataactact
    10081 ttgatgccgc acttagaact actgatatga atgaaagaaa tgcactctac aataaagcag
    10141 accaacaagt tattgatgat gccgtagtta tgccaactta ttacgacatt gattttcgat
    10201 tattgcaacc taacattaga aatttcccac aaaactcaat ggaatataga gatatggctg
    10261 aagtttatat gttgccacct actaagaaca aaaaataata gtatatgaaa atattttgta
    10321 ttggcttaaa ttacagagaa catattgatg aaatgaaaca tttttcattt cccgataatc
    10381 cagtaatttt tatgaagccg ccaactgcct tactaaaaga caaacaacct ttatattatc
    10441 ctgatttctc caatgatgtt cattatgaag gtgaaattgt attgaaaatt tcaaagaatg
    10501 gcaaaaaaat tgaagaaaaa tttgcacaca aatattatga tagtttttct ttaggttttg
    10561 attttacagc aagagatatt caagccgatt taaagaaaaa aggttggcct tgggaaattg
    10621 caaaaagctt tgaccaatct gcttgtattg gtgaatttat tcctttagat aaagaaaaaa
    10681 atatagattt tacaataaag aaaaataata ttttggtgca acatggtaat actgaaatgc
    10741 tcattttttc ttttgataaa attgtaagtt atgtttctca gtttttcact ttgcaacaag
    10801 gcgatttaat atatacagga acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10921 nngtttgctt tctaagtagt aaagcagcat ttattttaat tccgatggat aatacacaaa
    10981 aaaatcacct aaaagcttat ggtattgcat atttagcact aacaaaagac attcctcttg
    11041 attggctact aaattataga ggtggttctt ttgcattacc tcaaaattct atattagaga
    11101 aagaatgttt gattcgaaat gtaagttatg aagtcattcc tgatgcaaaa atgtcatcta
    11161 tacttactga aattagcaat cctgaagtaa atatggacgc agttcgattg gaaaaagctc
    11221 caaaagttgc ggtttattct cctaaatcaa aactcccttg ggacgatgca gttacacttg
    11281 ctttaactta tgcagaaatt ccgtatgacg taatttacga tgaagaaatt ttagaaggca
    11341 aattgcactt atacgattgg ttacacttac atcatgaaga ttttactgga caatatggta
    11401 aattttggtc tgcttatcaa tttgcagatt ggtacaagga acaagtcgca gaatatgaaa
    11461 tgcgtgctaa aaaattaggt tttaataaag tttccgatga gaaaagagct gttgctctaa
    11521 aaattaaaga ttttgtcatt ggtggtggtt tcttatttgc tatgtgtagt gcaacggact
    11581 cttatgatat tgcgttagca actgaaggca ttgatatttg tgatgtcatg tttgatggcg
    11641 acccaatgga cccacatgca caagacaaaa ttgattatca taaaggattt gcttttaaag
    11701 attatgaatt aagtacgaat ccttatgaat atgaatattc tgaaatagat gcattacctt
    11761 tgggcagaga ccaaagtacc gactattttt cattatttga tttttctgcc aaatgggatc
    11821 aagtgccaag tatgcttaca caaaatcatg aaaaaatatt gaaaggtttt atgggacaaa
    11881 caactgcatt tagaaatcct tttgtaaaaa ctgaagtttt ggttcttgga gaaaataaaa
    11941 gtttagatga agcaaaatat atccatggaa ctcaaggtaa aggcacttgg acgttttatg
    12001 gtggacatga ccctgaagat tatcaacatc gagttggcga ccctccaaca gacttaaatt
    12061 tgcacccaaa ttctccggga tatagattaa ttttaaacaa tattttattc ccagcagcta
    12121 aaaagaaaaa acagaaaaca taattaaaaa aataacatga acatacttaa aaatttcatc
    12181 tcaatttgca cagtaatttt actgagctat catgcttatg ggcaagaaat aaagcaaaca
    12241 gaatactcat ttgacattaa taaaactatt gatggtacag attactttta ttacttcaat
    12301 aaagggaaaa atgacacttt cattaatctc aaaataatga cttttaacat tgacaatact
    12361 gtaaaaactt tatgtaaaga agtttctgtc ttgccagaat tctacaatat tgcaacacaa
    12421 aaaatcaaga tagatttatg gaatatgaat gtaggcaatc ctttagaata tgaggatgtt
    12481 ttaaacaacc aaattaatgt atttgcaaaa gacactagtt ggcaaaatta ttacaaagta
    12541 tttagtaatc aagcgtctaa aaacaaaaat attcaattag attataatct aatttcaaaa
    12601 attatgacag accaaaaagt gtatggttgt atagaattag aattaaataa aattggatat
    12661 acaatttcac aagttggaat agaaaaagta ggtttcttag atttaaaatc atatcctgct
    12721 atcaaaaaaa gattaagtgg aaaattacca tattctgaca gtcaattaaa aacaatacct
    12781 attccttata ttgtctatat tggtataaat agaatatcct aaaatatgag aaaactagga
    12841 ttgttattct ttactttttt tgcactgata gtaattgctg gtccaccaga gattcctgag
    12901 ccaaaagtca aaaatgcaac aatcttagat gcacaaacga ttattattaa tctaaatgaa
    12961 gatgataata ttcaggaaaa tgcaaaccat caagatattc ttatatctgg aattagtaac
    13021 aaaattaaca taaaaggcaa ttgtaaaaaa gtgcttattt caggcatgga caatgaaatc
    13081 attttagata cagtacaaga aataatttta aaaggtaaca gaaatacgct tacatggaat
    13141 tacagtaaat taccagataa caaaccaata ataacagaca aaggttttca aaataaaata
    13201 gaacaaaaaa ccattgaata atgaatacat tatacctcat tccaacatta ctttctgaaa
    13261 atagtgcatc aagtattcct gaatatatta caccaatcat tgaaaaatgt gatgtattta
    13321 ttgttgaaaa tttaaaaact gcacgtcgtt ttatcaaatt aatactcaaa gaaaaagata
    13381 ttgatgtctg tacatttatc gaaatggata aacataaaaa ttattctatt gatgaacaag
    13441 aattgtatct ttttaaagat acaaatattg gattaatgag cgaggcaggt gctccttgta
    13501 ttgcagatcc aggtaataaa atcgtactat tagcacacga attaaattgg aaaattgtgc
    13561 ctttggtagg tccaagttct attcttttag ctttaatcgg tagtggtttt aatggacaaa
    13621 gtttttcgtt taatggctat ttaccttttg atgcaaaaga aagaaaatca aagattttag
    13681 aaatggaacg tttggctaac aaaaacatta cacaaatatt tatggatacg ccgtatcgta
    13741 acgtaaagtt attagaagaa ataatacatt gttgtagtaa aaatacttta ctatcgattt
    13801 cttgcaacct tacagctcaa gatgaattta cctatacgaa gactatcgga acttggaaaa
    13861 atacaaaaat tgatattcac aaaaaacctt gtatttttgt acttggaaaa taagtagtgc
    13921 tatgatttta attgatgatg tattaataag tgatgatatt attgaaaaac aattcgtttg
    13981 taatattgac aaatgcaagg gtgcttgttg tgtagaaggt gatagcggtg ctccagttgc
    14041 tgatgatgaa agaaaaatac tagaagaaat ttattcaaaa ataaaagcat atttaacgga
    14101 cgaaggcatt aaagccatag aagctcaggg aacatgggtt aaagaatctg atgatgaata
    14161 tgaaccttat gcgacacctt taataaatgg tggtgcttgt gcttatgtaa atttcgatga
    14221 aaaaggtgct gcttattgtg gcattgaaaa agcatataat gatggtatta tcaatttcaa
    14281 aaaaccaatt agttgccatt tatatcctat ccgattatca aaaatggaaa cagttattgc
    14341 aatgaattat gatgtttggg atatttgtaa agatgcttgt atattaggca aaaaactaaa
    14401 aatgcctttg tataaattct taaaagaacc tattataagg aagtttggag aagaatttta
    14461 tgaagtattg gaacaggtgg caaataatga aatagaacct gaataatata aaagcctttt
    14521 ctcatttaat agccaaaaat gaatgattat ttgctaaaaa ttaaattatt agctcccact
    14581 ttattaataa ccacattaag ttctattgtt ggattagcca tatttagatg ggtatttacc
    14641 attcattata acattattga tataccagaa gaaatttggg ggtattggat tccagtatcg
    14701 ctctcaggca tcattattat cttatgctta aaaaataaat ttaaaatttt tattaacccc
    14761 aaactatctg atgaaactaa acctagattt gcattattat tccttatatg gatttatttt
    14821 agtattctat taattatttc tcaagcgtat ttagaaactt catcatatag ccttattcaa
    14881 ttaaataata ttgatgaaat agaacaatct gataacaata aatattttaa aattgataac
    14941 ttttcaattg caaatactta tggaggttcg catactcata tttcgtatag tggaaaatat
    15001 aatcaacagc aatattttag tatatatttt gttattccta tctttaataa gaataaaact
    15061 aatcatccaa ttgttcctaa gtattggtat tctaaaaaat ttatatctaa atcattttca
    15121 aataaattaa gcaatattga aagaaataaa cagatttcaa aattctttat tgaatctgca
    15181 aaggagatgg atagtcttaa ttattataat tttgaatatt ttgaaaaagt acaaaattct
    15241 tttgaattag agaattatca taatgcaata aaatcaagaa taggacaaac cacacgcaat
    15301 aattttatag tattaaaacc tataaaagaa aaatttgagc atagaaatgg aaaaaaaatt
    15361 gaatggttta ttggtattta tattttagga tttggactat tcttactatt cataaaattt
    15421 gccaaacatg acaaacattt attaaatcaa tatatcaaaa catctaccta taaacaagac
    15481 aacatagata aaatacttcg atattttatt ccttcaaaaa ctcattttat atcttcaata
    15541 ttccttgata ttactagtat tgtattcgtt atatttttct ttttaagaaa taatatattt
    15601 catactaata aaaacgaatt actagaatgg ggtgcccttc aaaaagagga actttataat
    15661 ggagaattat atcgtttaat tacaaactta tttatccata gcagttatat ccattttcta
    15721 gtagatattt ttggttggat aataatagct gggtttgttg agaaaattat tggttataaa
    15781 aaactttgga ttagctcatt aattatcggt gttctttcta atcttataag tattactttg
    15841 gaatcaaata ttctttgtat aggttcaaca aatattataa tgggtataat tggaatacaa
    15901 cttatatttt tatctaaaca aatcaatact ccaccaataa aatcaggttt atttcttatt
    15961 cttatttgta ctttcttatc aattatttta attagtagtc taacaggtca tacaaatatt
    16021 agtgcaaata ttagtggttt tatagtagga atatcaatag ggtttatttt gtttaataat
    16081 tatgagtgga aaaaactatt ttagtaagta tggtaatata aataatacaa agttatcaaa
    16141 aattaaccat aagcaattat tgcttaataa tagaatgcaa ttttaaagat gccgacaata
    16201 atacaatttt tccattgaca tttctttcta tctcataata cttttcatct aacaattgga
    16261 taaactcatg aatctgttca tggtttttta aatatttttt aagagcctgt gcaattttaa
    16321 gctcctcatc atttagtttt gggatataag catcttgata tttagtcagt aatgtttctc
    16381 tcaaaacatg ctctgtatat tcaaaaaagg ttttaatttt ttctctaccc attttagaca
    16441 aatcatctac ccattttacc aatgctagaa tatcataatg aattgataac ttcatccaat
    16501 caactaaagt ataagtaata tcatcaatct ccttctctgc gatttcaaga gcttcagaaa
    16561 tgcttccatt agataaaaat gcaatttttt gagcttttgt actaggaata ccatttttta
    16621 ctaaagcctc ttcaatttct gactcactca attggtgcaa ttttatcaat tggcatcttg
    16681 aaataatcgt tggcaaaatc tgatctggat tttcagaaat caacagtaaa atagtatctt
    16741 ctgggggttc ttctaataat ttcaacaaga tattaccttc ttttcccaaa gcctctgcta
    16801 gccaaataat ttgaatttta aacctagatt cataagattt taagcttaaa tctcggatta
    16861 tagttctagc ttcatcagca gagatattgc cttgtctgtt ctcggcttct atattttgca
    16921 accatttgct ttcatcaata tatgcattgt tcaaaataat ctttcgccat tctacaatat
    16981 aatccgtact caaactaggc ttattgcttt ttttaggtac tacaggataa gtataatgaa
    17041 tatcaggatg aatcatttta cttgatttga tacattcacc acaaacgcca caactatctt
    17101 ccgcatgctt gttttcacaa acaagatatt gagcaaacgc taatgttaaa ggcaaactcc
    17161 ctacgcccga actactcaag aatagttgtg catgtggaat attattactc tgcaccaagc
    17221 caatcaaatg ctttttaatc tcttcgtgtc caattacctg atgaaattgc atttggctaa
    17281 attaagcata taaaaataaa ttattgcttt tgttaaaaaa agatttcatg aataataatt
    17341 ctaaaaacat aattcagtag ataaatggaa agtaattgta aacatttaat tttctagttt
    17401 atactttcta tctttgcaaa atgactgata tacgtgttcg ttttgctcct agtccgactg
    17461 gacctttaca tcttggaggt gttcgtactg cattgtacaa ctatcttttt gctaaaaaaa
    17521 ataaaggcac ttttatactt agaatagaag ataccgacca aaatagattt gttccaggtg
    17581 ctgaaaaata tataatggac agtttagctt ggtgtggtat aaaaatagat gaaggcatta
    17641 atgaaggcgg tgacttcgga ccatataaac aaagtgaaag aaaagaatta tacaaacaat
    17701 atgctgatgc tttaatacaa agtggacaag cttattatgc tttcgatacc gctgaagaat
    17761 tggaacaact aagaaaagaa aaccctaatt ttgcctacaa ttcaaaggtt agaggtcaat
    17821 taaataatag tttaaattta gataacgatt ctatacaaaa taaattaaat agtggaatac
    17881 cttatgtaat tcgtatcaaa gttcctgctg aaaccgagat tagttttaat gatattgttc
    17941 gtggtgcggt tagttttcat tcatctcaaa ttgatgataa agtgatgttt aaaagtgatg
    18001 gaatgcctac ctatcattta gccaatgtcg ttgatgacca tttaatgaaa atctcacacg
    18061 ttattcgagg agaagagtgg ctaccttcta cacctattca cgtattattg tatgaatttt
    18121 taggttggag tgcaacaatg ccacattttg cacatttgcc acttatttta aaacctgatg
    18181 gcaacggaaa actttcaaaa agagatggtg atagattagg ttttcctgtt tttccgctca
    18241 attggacaaa tcctgaaagc ggtgaagaaa gtcaagggtt taaagaacga ggtttcttac
    18301 ctgaggcatt tgtaaacttc ttagccttct taggttggaa tccaggtaca gaccaagaga
    18361 ttttatccat gcaagaattg atagaattat tcaatattga gaaactacat aaatcaggtg
    18421 caaaatttga ttttgaaaaa gcaaaatggt ataatgcgtc ttatataaaa ttattagaaa
    18481 gcagcactat tgcatcttgg ttacaagctc aaaatcaatt tcaaaatata gatttagact
    18541 acttaacaaa ggctatagag cttgtgaaag agcgtttaat tttccttcaa gacattcaac
    18601 attttaatta tctttttgat attcctaata attacgatga aactaattta gcaaaattag
    18661 gtactataaa aagtaatttc aacatcaagc aatttgaaaa tacgataaat aatatagcaa
    18721 cttttaattc ctcttcaatt gaagcatcga taaaggaatt tgcagaaaac catcaaatta
    18781 aagtaggtga aatgatgaag tttttaagaa taagtttggt tggagaacta tcaggtccag
    18841 ccatacctga tttaattgtt ttgttaggta aagttaatac tttaaaccgc atagaattag
    18901 gattagcgaa atgccaataa tcttgaatat agagctattg tattcattta caaatcataa
    18961 gatttaataa tgatttgata tgaagattca atagaaaata gtaaatattc gtatcttaca
    19021 aaatcaattc aaacaataaa aaactaaata tgagagaagt atttatcgta tcatttgcaa
    19081 gaacaccaat tggaagttta ggtggtgttt tagcaagtgt taataccgta gaattaggta
    19141 aaattgctgt acaagcagca atggaaagag ccaaagtaaa tggagatatg gttgacgaag
    19201 taattttagg taacattctt caagcaaatg ttggtcaagc acctgcacaa caagtttcta
    19261 ttgctgctgg catcaacacc aaaacacctt gtacaactgt caataaagta tgtgcttctg
    19321 gcatgaaagc aattatgtta ggtgctcaat ctattcaatt aggagataat gatgttgtag
    19381 tcgttggtgg aatggaaagc atgagtaatg ctccgcatta tttaccaagt ggaagaactg
    19441 gaattagata tggaaatgga gaaattatag atgctattgt tcgtgatggt ttacaagatc
    19501 catataaaaa atatatgatg ggcaatgctg gagaagtttg tgcaaagcac tattctttca
    19561 gcagagaaga tcaagataat tatgcaattg catcttatga aagagctgct gaggcatata
    19621 aaaatggcta tttcaatgag gagattattc ctgttacgat tgcaacaaga aaaggaaata
    19681 tcacaatttc tgaagatgaa gaatataaaa aattcgataa agaaaaagtt ggtacattaa
    19741 aacctgcatt tgaaaaggaa ggcacaatta cagctatcaa tgcttcaaaa attaatgatg
    19801 gagcttcggc aatggtttta atgagtggcg aaaaagtaaa agaattaggc ttaacgccat
    19861 tagcaaaaat agtagcttat gctgatgcct ctcaagaacc agaatggttt acaacaacgc
    19921 cgtcaaaagc aattcctaaa gcattagcaa aagcaaacat agacatcaaa tccgtagatt
    19981 attttgaaat taatgaagca ttttcagtag ttgcattagc caatatgaaa gaaatgtcta
    20041 tcgcacacga aaaagtcaat gcgtttggtg gtgctgtagc attgggacat ccaataggta
    20101 attctggttg tagaattgtg ggtacattaa tttctatttt aaaacacaaa aatggaaaaa
    20161 tcggagctgc aggaatctgt aatggcggtg gcggtgcaag tgccattatt attgaaaaag
    20221 tttaataaaa atagtataaa ttaaaaagag gctgtccgaa aatttagaca gcctcttttt
    20281 aatttatttc tttcgtaatt caaaactctg acccagataa actttacgca ccatttcatc
    20341 attggctaaa tcctcagccg ttccttgttt tataatttta ccttcaaata ataaataggc
    20401 tctatctgta atcgataaag tttcatgtac attatggtct gttataagaa taccaatatt
    20461 tttttctttc aatttttcta caatatgctg aatatcttct acggcaattg ggtcaatacc
    20521 agcaaatggt tcatctaata aaataaactt ggggtctgat gccaatgcac gagcaatttc
    20581 agttcttcta cgctctccac ctgaaagtaa ttttccttta gattttcgta ttttttgtaa
    20641 tccaaattca tctaaaagtg cttctaattt ttcagcttgt tgttgcttat tcaaatcaga
    20701 aaattctagt attgctttaa tattatcttc aacacttaat gttctaaaaa cagaattttc
    20761 ttgtggcaga tagccaactc catattttgc tcttttatac attggcacct tagaaatatt
    20821 atgattatct aaaaaaacat tgcctttgtt tggtgtaacc aaaccaactg tcatataaaa
    20881 agtagttgtt tttcctgctc cgtttggtcc caacaaacca acaacttcgc cttgttttac
    20941 ttcgatagaa acattatcta caacatttct gctgccataa gttttaaata aattttctgc
    21001 acgcagtacc atcttctatt caatatcttt aagtctaagt tctattttaa tttgattatt
    21061 ccattcatta gcatataatt gataacaaac atcaaaagta tctttatttt ccattatttt
    21121 atacaaatct gccataccaa aaccaatacc attaactgga ttttgtccat ctttcaataa
    21181 attaatttta agatgtgcct ctttaaccac ttttgaaaaa ccagtatcgt aaacatcttt
    21241 tgtaacaaaa gtaggtcgca tattttctgg accaaaaggt gccatttgtt caataatatt
    21301 atagaactta tctgtaatat ctgaaaactc aatttcagcg tcaatatcaa ttggagcaat
    21361 caataattct tctgtaatat tatgctgcac aacagattca aacatttctg taaatacatc
    21421 aattttatct aattcaattg ttaaacctgc agcatattta tgtccgccaa actgtattaa
    21481 atgttcacta cattgatcga ttgcttcgta taaatcaaaa ccaggcacag agcgaccaga
    21541 accaacacct tttccattcg actccgtcaa aataatagtg ggtctatgat atttatctat
    21601 caatcttgag gcaacaattc caataacgcc tttatgccaa tgtggtcgaa ataaaacagt
    21661 agatttcttt tgaattaata gttcactttg ctcaattaaa gtaaaagctt cttccgttgt
    21721 atctttatca aattcctttc tagtatcatt attctcttgg acttttttga tttttgtaat
    21781 ggccgtttct aaatctttag cgactaacaa ttcaacagct tccgaaccat gagctattct
    21841 tccagcagca ttaattctag gaccaattat aaatactaaa tcagtaattt ctaattttct
    21901 attcaatttc aattcgttaa ttagtgcttg aatacctggt ctaggttttt tattgaccaa
    21961 atcaacacca aattttgcca aaatacgatt ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22081 nnnnnnnnnn nnggtctagg ttttttattg accaaatcaa caccaaattt tgccaaaata
    22141 cgattttcac cagttacatg aactaaatca gaagctatac ttgtagcaac taaatccaat
    22201 tcttcatata tctgctcttt attgatatta tttttttcag cataagcctg aattaactta
    22261 aaaccaattc cacagccaga caattctttg taaggataat tgcaatctgg tcttttggga
    22321 tctaaaacag ctacggcatc tggaatcgtt tcagcaggaa gatggtgatc gcaaattata
    22381 aaatctatcc ctaatgactt ggcataatta attttttcat gagctttgat accacaatct
    22441 aaaacaataa tcaaagatac ttgatgttgt tttgcaaaat caattccttg tattgaaatt
    22501 ccataacctt cagaatatct atcaggaata tatgtgatta aattgggata aattttacta
    22561 aaaaaagaat aaaccaaagc aactgaagta gttccatcta catcataatc accatagatt
    22621 aaaacatttt cgttatgttg aattgccttt tcaattctat caatagcttt atccatatcc
    22681 ttcataagaa atggattgtg caaatctttt aaactcggtc taaaaaattg tttagcttca
    22741 tcataagtag atattcctct caaaactaat aatctgcata atacaggatg aattttgagt
    22801 acatcttgta aatggcgaac tttttgttca tcagcatcta aaaaaatcca tcttttttgt
    22861 tcactcatat ctcgctaagt taaaaaaata tttccttttt actaaacctt caaaaatatt
    22921 ctattattct tagcctaaaa ctcaaaatgg aatggtactt ttgtactata ttaaatgttt
    22981 tatgaaaaag ttgatttatc atgcagtggg tacaactcat ttgatacaag agaaaattaa
    23041 agaattacta gaagacttaa tccaaaacca acattatacg gaagaagaag gcaaacgtat
    23101 tgttgatgat ttttttggag atattaaatc aaatatagaa actgtaaagt tttcaatgga
    23161 ggtcaaatta gatgagctat tcaaaaaatt aaatatcgat tcgttcacaa cattgaaaag
    23221 taatatcaac caagttataa aagatattga agacaatcca tttttaccta ctatcatcaa
    23281 ccacaagaaa taagcgtcta tttaatctat gaaaacaatt gaactacata attacaatat
    23341 catatttgat gatactttca aatcattaga gcgtttttta gattcaaaaa attattctaa
    23401 aaaaattgta cttgtagatg aaaatactaa ggagcattgt ttacctattt tttcaaaaaa
    23461 tattaaatcg gatgttgtta ttgaaattca atctggtgaa gcaaataaaa acattgatac
    23521 ttgtgaacta atttggaaat ccttaattga ttataccata gatagaaatg ctgtatttat
    23581 aaacttaggc ggtggtgtaa ttggcgatat gggtggtttt gctgcaagtt gttataaacg
    23641 tggcattgat ttcattcaaa taccaacaac agttttatct caggtagatt cttctattgg
    23701 tggaaaatta ggcattgatt ttaaatatgg aaaaaattta attggcttat tccaaaatcc
    23761 taaattagtt tatatcaaca aagaatttct taaaacatta cccaaaagac aatacatcaa
    23821 tggatttgct gaaattttca aacatgcatt aattcaagat gctgtacatt ggcaaacact
    23881 caaaaatatt gatatctata atgaaaacat tgatgaagta ttgtaccaat ctttattagt
    23941 taagaaacaa gttgttgaag ctgacccatt tgaaaaagga ttgcgaaaaa tattaaattt
    24001 tggacatact attggtcatg ctattgaagc atattcatta gaaaatgaag ccaatccgct
    24061 tttgcacggt gaggcaattg ctattggaat gatttgcgaa gcctatttat cttataaaaa
    24121 aactggttta tcggaaactg agcttagaga aattcatcaa acattattat catacttccc
    24181 aaaatacaat atctcaaaat ttccaacaga taaaattatc caatccatga tgatggataa
    24241 aaaaaataaa ggaaaaaata ttcaaaccgc attattaaat aaaattggta attgtgtctt
    24301 tgatattaat attattgaga aagaaattgc agacagtctt gatttttaca aattctcata
    24361 aaataattca actttttata ccaaaactca atattttttc aaaaaatgta aaaaatttta
    24421 cataaatttt acagtattta aaaaatattc ttatacattt acattaaaat atagatatat
    24481 gaacgaaata ctacagcgcg aagccgcaat tacaatcgtc aaagacacat tcacaagatt
    24541 tctttgtgat tatttacacc taacaaaaat ctcatctcca gttgccgttt tagatggcac
    24601 aggaatcaat gatgacttaa atggaattga gcgtccagta tctttcccaa ttaaggcttt
    24661 aaatgaacag agagcagttg ttgtgcattc tttagcaaaa tggaaacgct tacgtctaaa
    24721 agaattagaa gttgaagctg gaagaggaat tttaactgat atgcgtgcat tacgtccaga
    24781 tgaagattac agtccaattc actcaattta tgtagatcaa tgggattggg aaaaacacat
    24841 taagcctgaa aatagaaata ttgattactt aaaagatact gtaaaaagaa tttatagtgc
    24901 aattaaggaa actgaaaaag ctattcaaca atattatcca aatgaagaac cagtattacc
    24961 tgaagaaata ttttttattc attcagaaga gttattgcaa aaatatcctg gattaacacc
    25021 aaaagaaagg gaaaatgcta taaccaaaga acatggtgct gtatttatta tgggaattgg
    25081 tggagcatta tctaatggtg aagctcatga tggaagagcc gcagactacg acgattggag
    25141 tacattaaac caagatggtt tcaatggctt aaacggagat attattcttt ggcatccaaa
    25201 attacaatca gcatttgaag tttcatcaat gggtattcgt gtagataaat ctgcattaga
    25261 gaaacaatta aaaattaaag gtatggaaga taaagcaaaa ttatctttcc atagtatgct
    25321 attaaatggc gaattaactg aaagtatagg tggcggaatt ggtcagtcca gaatatgtat
    25381 gtttatgtta aataaatctc atatcggaga agtacaagta agtatttggc ctgaagaaga
    25441 aagacaaaaa ttacaagctc agggaattac tttattgtaa gcaacctttt agttttattt
    25501 cattgtaaaa gtcttcgtac ataggaacta tattttcaat tttgaattgc agtgcgtttt
    25561 gataagcatt tcttctaaat tgctccaata tttcctcatt ttgcaataga tacaaacatt
    25621 tttcaaccat accatcaaca tctccaacag gcaagacaaa tccagtcttg cctgttatat
    25681 ttac
//
LOCUS       DAOQCW010000021        25405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_728_length_25405_cov_6.810966, whole genome shotgun sequence.
ACCESSION   DAOQCW010000021 DAOQCW010000000
VERSION     DAOQCW010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 25405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25405
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_728_length_25405_cov_6.810966"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PLS16_03270"
     CDS             complement(<1..415)
                     /locus_tag="PLS16_03270"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ60131.1"
                     /translation="MNTSSLIPTEKIIERLRYENPWWINKQIPETYKSMSKRLYFDLF
                     YPYVIEKKVRRALVLMGPRRVGKTVMLFHSIQQLIDKQINPQKIFFVGIDNPIYVHLS
                     LEDILSLCKQSLNQENLNGCYVFFDEIQYLKDWERH"
     gene            complement(866..1285)
                     /locus_tag="PLS16_03275"
     CDS             complement(866..1285)
                     /locus_tag="PLS16_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60132.1"
                     /translation="MESKIQTYKDIKTQSDAVLYFLQHLDIEMIDSVLEPNRTYQDFE
                     KGVFIKKLDCALDEFIQSGDTYLNRYSGQCNSESCNFKCKGFTFIGNHSGNYFDLIVD
                     IKEGVVLDIYECIRFKCFEESIVKNKRIEIDKMALPF"
     gene            complement(1449..2483)
                     /locus_tag="PLS16_03280"
     CDS             complement(1449..2483)
                     /locus_tag="PLS16_03280"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNJ60133.1"
                     /translation="MNKFQDNFKQIIFFIIIITLGIILFIPLRVIFAGFLGACTLYIL
                     NFRFVEFLSIKKKWNIYLVTFLIMFLNIVILLIPTGLAVMFVIPKLSTLFNNSNEFIT
                     GIKQIIFEIESRFNIEIISTSHLDKLTSVASSIIPEILGTISSFLLNISTMFFILYFM
                     LVNSKSIGRTIRNKIPLKPENLDSISKETRNVIQSYAIGIPIISLFQGLAATIGYAIF
                     NVPDIYLWGFITAIVAVIPFVGTGLVAVPMIAYLFAVGETYNAIGLILYTGFVVMQID
                     NLLRLFLLKAFANIHPLITLCGVIVGLKLFGFIGLIFGPLLVSYFILLIKIYNIEFAP
                     TNNSASEKKS"
     gene            complement(2476..3564)
                     /locus_tag="PLS16_03285"
     CDS             complement(2476..3564)
                     /locus_tag="PLS16_03285"
                     /inference="COORDINATES: protein motif:HMM:NF016277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60134.1"
                     /translation="MLAPVEYYYPETRIEKTFLDKLLALGYFRTANYMLRTRVMLYNS
                     ELYNLFHIRYHLNTYQFPKSLRKLYNKNIKHFYHKISPFVNTKEKEFLFSKHQVRFKG
                     SNTSSLHKYLFDCENFSLFNTYQVEIFDKSTNQLVAYSIFDIGTNSLASILGIFDSRY
                     EAFSLGLYSMILEVQYGKEQNFNYYYPGYIADKPSPFNYKTRLGKRPEYYNWFDDTWY
                     ALDDIEKESKVWDYYNSQLAIAEDWLKTNNIPYIKTRNPYYYNDYYYAGNMSLSSIEQ
                     LLILDFSIPETYFIVEYNPTKNCIIIASIEPNILNEDEALSINDLLQNNIWKTLMLYK
                     HPIYFAENAKDIKLILTALKKNKTNKHE"
     gene            complement(3548..6511)
                     /locus_tag="PLS16_03290"
     CDS             complement(3548..6511)
                     /locus_tag="PLS16_03290"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNJ60135.1"
                     /translation="MLKKLLSISLVFIFLISFSKDVSFEKGVQLFELEAYQQAKKMFQ
                     QAVQNENSRLKILECNFMLQNFETFEQELTAFRKSIRDSSLIEQADLLQLQYLLNTNN
                     LEKAHFFAEQYTPKTIFNKGSKAFLLGTIYLRKNQFELAKSNFTNAINLLSKFKESKY
                     IRKCGMATNGIAVVYYYQSEIEKAMQYYLKAKELFQKFPHHNATEIARVNYNLSLIYF
                     EQNRTEEAIHLVKNALEINKKKFGENHKRVAECYSVLGSIYLEKGELDKALDYFTRDK
                     EICEAIYGKEHIEAAYAYFDCGMVYSELKDYYHAQYYLEKALAIRQKFLEENNEDISE
                     NYLELAIIYIALQKYDEARLLCKKVISNEQQLGTENSMRMAESYYRIGLIDKVQFKYN
                     EAIKNFNIAIGIYNTSLGIKNNYNLGIYLNIAEIKLDEKKYNEALLFTKKALPDNKND
                     LFPVTLAYLQYLKIIQQRDLNKSFAIEQNKKYLEILIGLTNKIQDRKFEYIGEKSKLS
                     DAEILAQISAIAIPLTEQLYQLENKKEYLEWMYFFIENNKANALNSNLKNKNNNSSII
                     KKIEQINAEIEQQSLNETNDSKIKQLKNELFILKKQYELETKKVNNSNIKEGKNTIPT
                     IQQKLNPKTAFLDYYNQGQSTYLLAITNNNSTLYKIDLSEDSIKILNNSIQAKKYDKE
                     LAKKITRQLIPTKILDENIKLIIAPDGMTRNISFGALIHQNKYLIEQYNISHTFSGRI
                     YFGNDNKKQLTNDKVLCIAPNYKQSNYTPLQNIDEIKLIQPLLQSKILVAKDANIDNY
                     NLNKDNFGIIHFSAHSQIDSLIPTSSAIIFQPDSLHNYFLKISTIIQQNINTNLVSLN
                     MCQGGFGRKNSGEGILNFAWAFQYAGAKNILVTRWNASDKVAKTMMTYFYKNIKNKQS
                     YEDAIRNAKLAYLMNTDAMGAEPFFWSNYSIYSTFSKENHSMKYIIFTLTLLFLGILL
                     FYFKNISKKNNVSTS"
     gene            6579..7640
                     /gene="leuB"
                     /locus_tag="PLS16_03295"
     CDS             6579..7640
                     /gene="leuB"
                     /locus_tag="PLS16_03295"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNJ60136.1"
                     /translation="MEKKIAVLPGDGIGPEIITEAVKVLNAIAQKFGHQFQYEYGLIG
                     ADAIDKTGNPYPDATHELCLNADAILFGAIGDPKYDNDPSAKVRPEQGLLKMRGALGL
                     YANIRPVKSYDALLDASPLKNDRIKGADFVVVRELISGIYFGKPRGRSEDQNIAYDTC
                     VYTREEILRITEKAFEFAKNRNKKVTLLDKANVLATSRLWREVVTEYAKQFPEITLEC
                     NFIDSAAMEVITKPTKFDVILTENLFGDIISDEASVIGGSLGLLPSASMGEKTSLYEP
                     IHGSYPQAKGKNIANPMATILSAAMLLEYSFGLIEESKAIVNAVDKALNEGFVTEDIN
                     QGKYKTNEVGDKIVEFILQ"
     gene            7647..8771
                     /locus_tag="PLS16_03300"
     CDS             7647..8771
                     /locus_tag="PLS16_03300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ60137.1"
                     /translation="MKIAIIGPAYPYRGGIANFSERLAYELQLEQEKIQLFTFNLMYP
                     SFLFPGKSQYAESVLQFPLEITQNINSINPFNWWKVGKYIQKQNFDLVIIPYSIPFLA
                     PSLGFIARVIKKNSHTKILSIIHNLHPHEKRFGDKFLSNFFLKSCDVFLTLSSKVKED
                     ILGIRSNAKVIVSNHPIYDTFGAIIEKQKAKKQLNLEESKQYILFFGLVRAYKGLDIL
                     IDAMSIVAQQNPNIQLLIAGEFYGDYPKYLAQIEALNLSQRIVVINQFIPEDKVNSYF
                     CAADIVVQPYKNATQSGITQICYHFNKPMIVTNVGALAENVANNHVGFVCEPSKDAVA
                     ASILKFYQEEKEAIFIENIKEEKKKYSWHKLSQQIKSVFQ"
     gene            8775..9347
                     /locus_tag="PLS16_03305"
     CDS             8775..9347
                     /locus_tag="PLS16_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNJ60138.1"
                     /translation="MQEYIIQILKETIEQRNKLLNDAVLLGNIENIAQLLIQTFRQHN
                     KVLFCGNGGSAADAQHLAAEFSNKFMKDRKSLDAEALHVNTSYLTAVANDYSYRDVYA
                     RLIEARGQEKDVLIALSTSGNSENIVEAVKMAKQKQMVVVGWTGQNAGNMDNYCDILI
                     KVPSDNTARIQEMHITIGHIICQLVEAELF"
     gene            9418..9771
                     /locus_tag="PLS16_03310"
     CDS             9418..9771
                     /locus_tag="PLS16_03310"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNJ60139.1"
                     /translation="MKKLFTLIFIFISFNIFVSQNAIAKSLFAPDLKEILSIYPNPIA
                     TNATIKISNDINLQESNVSVVFYNIVGKEIFKISQIKNYEINISRDNFKTSGLYFYQL
                     NIDEKLQSTGKVIVK"
     gene            complement(9818..10954)
                     /locus_tag="PLS16_03315"
     CDS             complement(9818..10954)
                     /locus_tag="PLS16_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HNJ60140.1"
                     /translation="MFDFVEPITQEFYNECITRNPNLPNNVAFFGLDTTEIEPYKIAL
                     VGVEDNLNNDDNQGTELAPNQVRQQLYTYTSFYNDTKIIDLGNIKKGNTRQDTLVALK
                     DVTKNAIRNNIILIIIGGDLDLLQAQYNAYEILENEIQISQIGANMTANPEEGKLEES
                     YLYNIFNTNYLKKYNLIAYQSYFIQKDISTIVHNYQFDSLRVGKIRENVFEAEPFIRE
                     ANLAVFDIQAIRFSDAPGQVAPSPNGLFGDEACMLARFAGMSDILSSFGIYNYNPMED
                     IKNVTAHQVAQMIWYFIEGVNLRRMDYPILNEQEFYHFTVYVEEEAFTFLKSKKTERW
                     WMKINVNRGDRTVAHLIPCIYNDYRIAKDGEIPEAWMRNLIRLQ"
     gene            complement(11028..11669)
                     /locus_tag="PLS16_03320"
     CDS             complement(11028..11669)
                     /locus_tag="PLS16_03320"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNJ60141.1"
                     /translation="MIGNWKWKLAQSLEIKWWKNYLDNKDIQTYLKWKQQYWKDFITK
                     IPNIPTLENLTILDAGCGPAGIFTALKNNKVYALDPLLDEYKKLAHFDPVWYPYTQFI
                     TQSIEQIETKEEYDVIFCLNAINHVENIELAYKKLAEALKPNGWLIVSIDAHRNAFLH
                     KLFKILPGDVLHPHQYNLKEYEGFMAQNKINLLPSILIKKEFIFDYYVTYGKK"
     gene            complement(11717..12493)
                     /locus_tag="PLS16_03325"
     CDS             complement(11717..12493)
                     /locus_tag="PLS16_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNJ60142.1"
                     /translation="MFLIDTHTHLYEDNFNLDRDEMIQRAFDAGIQNMWIPNVDVSTI
                     EPMMKICRQYPQQIFPMLGLHPCYVKPENYQQELATIYDRIADIKPIAIGEIGIDLYW
                     DKTTLEIQKEAFETQCDWALEHQLPIAIHSRESTYIILDILKKRKQNPKGIFHCFTGS
                     LEEANEIIKLGFYLGIGGVVTYKNTHLRETLTNISLENIVLETDAPYLPPVPYRGKRN
                     ESAYTKIVAETLATIYQKTIEEIATQTTQNAKQLFNLKIS"
     gene            12606..13184
                     /locus_tag="PLS16_03330"
     CDS             12606..13184
                     /locus_tag="PLS16_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020046524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HNJ60143.1"
                     /translation="MMNEVQYFKHSSAIIDDGAIIGKDTKIWHFCHIMQANIGSNCIL
                     GQNVFVANNVKIGNNVKIQNNVSVFEGVTIEDDVFIGPSVVFTNVNNPRSFIERKEEY
                     KHTLVKKGATIGANATIVCGYTIGEYAFIGAGSVVTRNINNFELVIGNPARQKYWVCK
                     CAEPLHFENNEAFCAKEQKTYILESDIVTIKE"
     gene            13204..13512
                     /locus_tag="PLS16_03335"
     CDS             13204..13512
                     /locus_tag="PLS16_03335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNJ60144.1"
                     /translation="MNKALKYFNISSLENRLFSSLSFGEQRLVLFIRAIIKLPKLLIL
                     DEAYQGFDQNTISRCNTYIDEVLPKSTTLIFTSHYLEEIPKCISNFLFLSNGKIVSKN
                     "
     gene            13487..14347
                     /locus_tag="PLS16_03340"
     CDS             13487..14347
                     /locus_tag="PLS16_03340"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNJ60145.1"
                     /translation="MEKLSPKIKFAIIGVAGFVAPRHLQAIKNIDGDLLAAFDISDSV
                     GVLDSYFPDCDFFTSIDTFEKYIKQTKIDYLVVCSPNHLHIEHCLVGLRNGINVICEK
                     PLCLNSEDLRKLQEAEQASQKQIFTILQLRLHHEIIALKEYINPTVFYAGELNYYTPR
                     GNWYYESWKGIEAKSGGIATNIGVHFFDMLCWFFGELQEIIVLQKDAIEAKGILVFKN
                     AKIKWHLSIDRKLVAKRQIVLNGKEIVFTEGFNHLHTKCYEAIIAGRGFKTIEILSST
                     KIIEQIQAYH"
     gene            complement(14344..16242)
                     /locus_tag="PLS16_03345"
     CDS             complement(14344..16242)
                     /locus_tag="PLS16_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNJ60146.1"
                     /translation="MSDFIKHECGIALIRLLKPLEYYREKYGDIHYGLSKMYLLMEKQ
                     HNRGQDGAGIATIKLDTKPGAPYFDRERSVAKDAIKEIFGKVFKQFNNISEAEKIAIK
                     DMDYVKQNYAFMGELLMGHLRYGTHGGNDMSNCHPRVRANNWKTRTLIVAGNFNMTNV
                     DELFEKLVSLGQFPREASDTITVLEKIGHFLDSENQKLFRTYKSERNLQNVDITPLIA
                     ENMNVEKILKNATDDFDGGYAMAGMIGHGDAFALRDPNGIRPAYYYKDDEVVVVASER
                     PAIITTFNVHRSKIKELQPGHALIIKKDGSCGEVLCSPPREKKSCSFERIYFSRGNDY
                     EIYQERKNLGKLLVPKILERIQYNLKDTVFSYIPNTAEVAFLGMVEEIQKKHIEYNLD
                     LYNKQTIDFNELQANLKIMPRVEKIAVKDVKLRTFITDDNSRNDMVHHVYDVTYGLVS
                     NNVDTLVVIDDSIVRGTTLKESILTMLDRLQPKKVIIVSSAPQIRYPDCYGIDMSKMG
                     TFVAFQAAINLWKKSNKASVVQEIYKKCIAELEKPISEMQNLVKEIYAPFSDEDISNE
                     IAEIVRPKHIFSDIEVIYQTIDNLHIACPKNLGDWYFTGNYPTPGGNKVVLKSFVNFI
                     EGKNVRAY"
     gene            complement(16325..16975)
                     /locus_tag="PLS16_03350"
     CDS             complement(16325..16975)
                     /locus_tag="PLS16_03350"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HNJ60147.1"
                     /translation="MELPNISNIISENIPNLLPQSKLLLSLALGLGLSASSGFRVFVP
                     LLVASIASKLGIIHLGESFQWMGSTTAIVAFATATVLEIGGYYIPFVDNILDTITTPT
                     SVIAGTLLTASVLTPELDPMLKWGLGIIVGGGSAGIVQAGTALTRATSSVATAGVGNP
                     VVATTEHGLSFAGSILSIFMPFIIAAIICIVLFVFLFFLIRYFKKRIYKPKATLSA"
     gene            complement(17025..19490)
                     /gene="priA"
                     /locus_tag="PLS16_03355"
     CDS             complement(17025..19490)
                     /gene="priA"
                     /locus_tag="PLS16_03355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNJ60148.1"
                     /translation="MDIFEKYDIFVEVILPLALPKQYTYGVPKELEQELGIGKRVEIQ
                     FGKHKIYSALICKIHQNPPKDYQAKPILSVLDEEPVITEKQLQFWEWMSSYYMCTLGE
                     IMNASLPSYLKLDSESIYIKNDLIDYNELHLSEDAQIICKAFEFQPSLSLKDIQLIVK
                     KKHVYKTIKELIFNRLIFIEEILYEKYQIKKEIFVRWHQDIIDNNQKASCFELVKNAP
                     KQEQILLGFIDLERKDKRIKRSDLLKRVDATSATLNTLVEKRIFEIYEEEVDRITIDK
                     SEHKNQFSLNEEQNKAIQQIDEAFQVQKPVLLHGITSSGKTLVYTQIIKNLKHQNQQI
                     LFLLPEIALTAQLIQRLQNWLGNIAVYHSKLSNSERVEIWNKVLKQEIQIIVGARSAL
                     LLPFSNLGLIIVDEEHDASYKQQEPNPRYHARDAALMLANMHKANVILGSATPSFESY
                     FNARQNKYQLVELTKRYNDVPLPEIKFINYKNAQKEKKFVSGITFELRDKIQDTLNEK
                     KQVVLFQNRRGYAPYLSCKSCDWIPMCKNCDVTLTYHKYTNDLRCHYCGYTETHISHC
                     KACGDNNLEQKGIGTERIEDDLKSLFPDAKIGRMDYDTVKTKYGHEKIIHAFEDGVYD
                     ILVGTQMVTKGLDFKNLQLVGVLNADALLYFPDFRAIERAYQLLIQVSGRVGRKDVKG
                     QVEIQISNEEHQIVQAVLKNDFKDFYLSQISERKLFSYPPYSRLIGITIRHKDLSIVE
                     EAAQLLSKQLQKHYNEWLKGPNKPIFQRINNYFIREILIKIPREHFTQLSHIKQMIDY
                     EIKNLYQFEKFKNVFIQKDVDLM"
     gene            complement(19605..21299)
                     /locus_tag="PLS16_03360"
     CDS             complement(19605..21299)
                     /locus_tag="PLS16_03360"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNJ60149.1"
                     /translation="MKTIYLLLILLSIAHISTAEDLFPFSNYNTKKKNEKTLYGFKDK
                     AGNVVVPLKYHSVYVFDEGFCKVILNGKIGLINDKGYEIVPPIYDDMGALGKNLVYVY
                     LNGKESVINMKGKLLAPLKYDKVLIDSSVIWVKLDGKWGIFDTIGNVIAPIQYTFYHT
                     EKPGRTRVFIGDKLGYIDEQTGKEIIPIIYNEISTFENGSSLAKRNNKFGCIDKETGK
                     EIIPVIYDEIIKYKEEPFLKAKLNGKWGLIDDKGKEVIPFKYENFFNYSDDIIDLVNV
                     ELDSKYGLFSLKLHKELITPQYDEIKLDGEREDKFIKVIAINSAKAKMGLIDTATTKL
                     FIPIKYDQISSYYDNLATVAIDTNRGFVNLKGIEVVPVKYKHMQGNLDPDPYELMKKD
                     ENGKNALDYANEISVEDGNLLAITIKNIFITDSIQKENIKNFNENMKANKLSSLPSWD
                     ASFNYDCTYSTINKEKDNANDILLNKKLEINVRKYFIIFRNMPEISEKYIDIPIMRSD
                     QETKVEEGMKLKYYYYFFEPNKYGILNVSFLLKDEIAYQVRIQKNKKTEAFKFFCK"
     gene            complement(21446..23482)
                     /locus_tag="PLS16_03365"
     CDS             complement(21446..23482)
                     /locus_tag="PLS16_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60150.1"
                     /translation="MLVGFLFSYLLYGRKSFIFFAEEKKSLRYILATLRFLAVSLIAF
                     LLLSPLIKTTQTEEQKPTIVLLQDNSASLKNVFKQSNLKQYQEKLNALTTQLGKKYNV
                     ITHQFGGNLSTNKTLDFTQQSTDIDNAIKQTFLKYDHLNIGAIILASDGIYNVGNNPV
                     YNPFATKTPIYTIALGDTTIKKDALIRNLLYPNIVYLTDNFNLQVDVDAHLLNGKNTI
                     LEVIDSNGKIVFNKSISIHQDDFSTSAQIVLQADKPGILKYHVRLKMIDGETISENNI
                     QTAYIEVIDGRQKILLLYGSPHPDVKAFKYAIESNKNYQIEVESADNFNKKLDKYDMI
                     ILHLSSSLDANLIQEISQSKLPILYILSAQANINTFNNLQNLLKISGSSLNGNDVFPI
                     PQSNFSKFVLDEQSTNILKQLPPILTPYGKYSVNPNADILFRQQLGKVPTNNPLILFA
                     DENNKKTGIFAGEGWWRWRLNEFMQFKNTDATDILINKSIQFLTVKTDKRKFKISTSK
                     QIYGTNEPIVLEAQLYNESFVLINEPEVNLELKNGKNAYRFVFDKTLNAYILNTGILP
                     AGDYTANAQTNYKGQQFKASTKFSVQPINIEVQNLQANWSILRTLAQQSGGKTYTVQN
                     LESIADAIQKDENIQSILFENTKTQPLIDWKILFGIILLLLSAEWIIRKYNGIA"
     gene            complement(23556..24425)
                     /gene="sucD"
                     /locus_tag="PLS16_03370"
     CDS             complement(23556..24425)
                     /gene="sucD"
                     /locus_tag="PLS16_03370"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNJ60151.1"
                     /translation="MSILVNKDSKVIVQGFTGKEGTFHAEQMIQYGTNVVGGVTPGKE
                     GQSHLDRPVFNTVENAVKQTGANVSIIFVPPAFAADAIMEAADAGIQLIVTITEGIPA
                     QDMIKAKAYVKQKGVRMIGPNCPGIITSEECKVGIMPGFVFKKGRVGIVSKSGTLTYE
                     AADQVVKAGYGISTAIGIGGDPIIGTTMKDAVEQFANDNETDAIVIIGEIGGTMEVDA
                     AYWIKDNFKKPVVGFIAGQTAPKGRRMGHAGAIIGGANDTAAAKMKIMGECGIHVVES
                     PAFLGKTLAEVLK"
     gene            complement(24422..25198)
                     /locus_tag="PLS16_03375"
     CDS             complement(24422..25198)
                     /locus_tag="PLS16_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNJ60152.1"
                     /translation="MIEVKDLYKSFGDQQVLKGITTTFLPGKTNLIIGQSGSGKTVML
                     KCVVGLLNPTEGHVLFDGQDYVSMDLDEKNKIRQQLGMLFQGSALFDSLTVEENVMFP
                     LEMFTKMSKSEKQDRVNEVLKRVNLVGANKKFPSQISGGMQKRTGIARAIVMNPKYLF
                     CDEPNSGLDPQTSIVVDELIKEITEEYNITTVINTHDMNSVVTHGEKILFLYKGNKHW
                     EGDKVSLFEAENKELLDFIFASDFLKEARNVRLDKLKKQI"
     gene            complement(25202..>25405)
                     /locus_tag="PLS16_03380"
     CDS             complement(25202..>25405)
                     /locus_tag="PLS16_03380"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ60153.1"
                     /translation="MTFEAKNIVVMLVKAAVFGFLLTSISCYQGFYAHGSALEIGKAS
                     TTAVVLSCVMVLFADLILAWLLL"
BASE COUNT         8316 a         3926 c         3736 g         9427 t
ORIGIN      
        1 gatgtctctc ccaatcttta agatattgta tttcatcaaa gaaaacataa catccattta
       61 agttttcttg gttgagtgat tgcttacaga gacttaaaat atcttctaaa cttagatgga
      121 catagatagg attgtcaatc cccacaaaaa atattttctg tggatttatt tgtttatcaa
      181 taagttgctg tatgctatga aatagcataa ctgtctttcc gacacgcctt ggtcccataa
      241 gcactaaagc tcgtcgtact ttcttttcga tcacataagg ataaaataag tcaaaatata
      301 atcgtttgga cattgatttg tatgtttcgg gtatttgttt gtttatccac catgggtttt
      361 cataccgtaa tcgttcaatt attttttctg tcggaataag gcttgaagtg ttcataatat
      421 ttatttgttt gtacaaatat agcaaattaa atagatttga atctgcttat tttgtacaat
      481 taatcattaa tagtagaata tttaatcaat aaaatgtttg tttaggtgtg ataggcattt
      541 ttaaaatttc tttttactca ctactaattt gtttttaaaa ggtgttcgtg aatactttaa
      601 gaccgttgca aggtaatcat tattcataaa tctcaagcca aattccttca atcttcttcg
      661 tttctcattt atttttaatc ctcattatca atatgataac tgcggtgtaa aaataaatga
      721 gtgccttgaa tatttttgta atttgacttt tgcaacggtc ttactttgta tttctcttgg
      781 gttggcattg taagctcttc gaaacgcttt ggtctgtgct tgggcttgtg gtgttttggg
      841 aatgtgctta caaatagctt tgactttaga atggtaaagc cattttgtct atttcaatac
      901 gtttattttt aactattgat tcttcaaagc atttgaatct aatgcattca taaatatcga
      961 gaacaacacc ttctttaatg tccacaatca agtcaaaata gttacccgaa tggttgccga
     1021 taaaggtgaa acctttacat ttaaaattgc aggattctga attgcattga ccagaataac
     1081 gattcaaata ggtatcgcct gattgaataa actcgtctaa agcacaatcg agttttttaa
     1141 taaaaacacc cttctcaaaa tcttgatatg tacgatttgg ctccaaaact gaatcaatca
     1201 tttcaatatc caagtgttgc aaaaagtata acacggcatc agactgtgtc tttatatcct
     1261 tatatgtttg aatttttgac tccatatatt gtcctaaaaa aggttaaagt atttttgttt
     1321 aaatcctaag ataaatatac attttatttt aatcctaaaa aagataacac ttttttattt
     1381 ttatatactt ttagtaatca atgtgtaaag ctgttttagg acaagacaaa ttataaatct
     1441 acaataaatt aggatttttt ctctgaagca ctattgtttg taggagcaaa ttctatattg
     1501 taaattttta tcagtaatat aaaataggaa accaaaagtg gtccaaaaat taagccgata
     1561 aatccgaaaa gttttaaacc gacgataaca ccacaaagtg tgattaatgg gtgaatgttt
     1621 gcaaatgctt ttaataaaaa taacctcaat aaattgtcaa tttgcatcac aacaaagcca
     1681 gtatatagaa ttaatccgat ggcattatag gtttcaccaa ctgcaaataa ataagcaatc
     1741 atgggtacgg caactaagcc tgttccaaca aatggaatta ctgcaacgat agctgtaata
     1801 aatccccaaa ggtaaatgtc tggtacattg aaaattgcat atccaatagt ggctgctaaa
     1861 ccttggaata aggaaataat tggtattcca atggcataag attgaataac atttctagtt
     1921 tctttgctta tactatctaa gttttcaggc tttagcggaa ttttatttct tattgttctg
     1981 cctatacttt ttgaattgac taacataaag tatagtataa agaacattgt acttatattg
     2041 agtaaaaatg aactaatagt gccgagtatt tctggaatta tacttgatgc aacggatgtt
     2101 aatttatcta agtggctagt cgaaataatt tcaatattga aacggctttc tatttcaaag
     2161 atgatttgtt tgatgccagt gataaactca tttgagttat tgaataaggt actcaatttt
     2221 ggaataacaa acattacagc taatcctgta ggaattaata aaatgacaat gtttagaaac
     2281 ataatcagaa aggtaaccaa atagatattc catttctttt tgatgctcaa aaattctaca
     2341 aatctgaagt ttaatatata caaagtacaa gcacctaaaa atccagcaaa aatgactctt
     2401 aatggaataa atagtataat tcctaaggta attatgataa tgaaaaaaat tatctgttta
     2461 aaattatctt ggaatttatt catgtttatt tgttttattc ttttttaaag ccgtcaaaat
     2521 gagttttata tcttttgcat tttctgcaaa atatattgga tgtttataca acatgagtgt
     2581 tttccaaata ttattttgta gtaaatcatt tatacttaat gcttcatctt cattcaagat
     2641 atttggttct attgacgcaa tgataataca attttttgta ggattgtact ctactataaa
     2701 ataagtttca ggtattgaaa aatcgagaat caataactgt tcaatagatg ataaactcat
     2761 attgccagca taataataat cattataata ataaggattt cgagttttta tataaggaat
     2821 attatttgtt tttagccaat cttcagcaat agcaagctgc gagttgtaat aatcccaaac
     2881 tttactttct ttctcgatat catcaagagc ataccaagta tcatcaaacc aattgtagta
     2941 ttctggtcgc tttcctaatc tggttttgta attaaatgga gatggtttat ccgcaatata
     3001 gcccggataa tagtaattga aattttgttc ttttccatat tgtacttcta aaatcattga
     3061 gtacaatcca agactaaatg cttcatatct ggaatcaaaa ataccaagaa tactagctaa
     3121 actattcgtt cctatatcaa aaatgctata tgccaccaat tgattcgttg atttgtcaaa
     3181 aatctctact tgataggtat tgaaaagtga aaaattttca caatcaaata ggtatttgtg
     3241 taaggaactt gtattggagc ctttaaatct tacttgatgt ttgctgaata aaaattcctt
     3301 ttctttagta ttgacgaatg gactaatctt atgatagaaa tgcttaatgt ttttattgta
     3361 taattttcta agacttttag ggaattgata ggtgtttaag tgataacgga tatggaataa
     3421 attatacaat tctgaattgt acaacattac tcttgttcgc aacatataat tagcagttct
     3481 aaagtagcct aatgcaagca acttatccaa gaaggttttt tctattcttg tttctggata
     3541 ataatattca actggtgcta acattatttt tcttacttat atttttaaaa tagaataata
     3601 aaattccaag gaataacaaa gttaaagtaa aaattatata tttcatacta tgattttctt
     3661 ttgaaaaagt actgtaaata gaatagtttg accaaaaaaa tggctcagca cccattgcat
     3721 cagtattcat taaataggct aatttggcat ttctaatggc atcttcataa gattgcttgt
     3781 ttttgatatt tttatagaaa taggtcatca ttgttttagc tactttatct gaagcattcc
     3841 aacgtgttac tagaatgttt ttcgcacctg catattgaaa tgcccatgca aaatttagaa
     3901 taccttcgcc agaatttttg cgaccaaaac caccttgaca catatttaag ctgactaaat
     3961 ttgtattgat attttgttga ataatcgtac tgatttttaa aaagtaattg tgtaagctat
     4021 caggttggaa aataatagca gaagaagttg gaattaaact gtcaatttga gaatgtgcag
     4081 agaaatgtat aataccaaag ttatctttgt ttagattgta attgtcaata tttgcatctt
     4141 ttgcaactaa tattttcgat tgaagtaaag gctgaataag cttgatttcg tcaatattct
     4201 gaagtggtgt ataattggat tgcttgtaat tgggagcaat acataaaact ttatcatttg
     4261 tgagttgttt tttgttgtca ttaccaaagt atattcttcc tgaaaaagtg tgactgatat
     4321 tatattgttc tattaagtat ttattttgat gaattaatgc cccgaatgaa atattccttg
     4381 tcattccatc tggtgcaata ataagtttaa tattttcatc taaaattttt gttggaatca
     4441 attgacgggt tattttttta gccaattctt tatcgtactt ttttgcctga atgctgttgt
     4501 ttaaaatttt tatagaatct tccgataggt caattttata taaggtacta ttgttatttg
     4561 taatggctaa taaataggtg gattgacctt ggttataata atctaagaaa gctgtttttg
     4621 gatttaattt ttgttggatt gtaggtattg tatttttacc ttccttaata tttgaattat
     4681 tgactttttt agtttccaac tcatattgtt ttttcaaaat aaataattca ttttttagtt
     4741 gctttatttt tgagtcattc gtttcgttta gactttgttg ttctatttca gcatttattt
     4801 gctcaatttt tttaattata ctactattgt tgtttttatt ttttaaattg ctgtttaggg
     4861 cattggcttt attattttca ataaagaaat acatccattc caaatattct tttttgtttt
     4921 ctaattggta taactgctca gttagtggaa tggctatcgc tgatatttga gctaaaatct
     4981 cagcatcact tagttttgat ttctcgccaa tatattcaaa tttcctatct tgaattttgt
     5041 ttgtgagtcc aattaaaatt tctaaatatt ttttgttttg ttcaatagca aaagatttat
     5101 ttaagtctcg ttgttgaatt atttttaagt attgtaaata agcaagtgtt acgggaaata
     5161 aatcattttt attatctggt aacgcttttt ttgtaaagag taatgcctca ttgtattttt
     5221 tttcgtctaa tttgatttca gcaatattta agtatattcc taaattgtaa ttattcttta
     5281 tgcctaaaga tgtattataa attccgatgg cgatgttgaa gttttttatt gcttcattat
     5341 acttaaattg gactttgtcg attaagccta ttcgataata ggattccgcc attctcatag
     5401 agttttcagt gcctaattgt tgttcgttgc ttattacttt tttacaaagc agtcttgctt
     5461 catcatattt ttgaagagca atataaataa tagccaattc taaatagttt tctgaaatat
     5521 cttcattgtt ttcttctaaa aacttttgtc tgatggctaa tgctttttct aaataatatt
     5581 gtgcatggta ataatctttt agttcgctat aaaccatacc acaatcaaaa taagcataag
     5641 cagcttcaat atgttctttg ccataaatag cttcgcaaat ttctttatct cgagtaaaat
     5701 aatctaaggc tttgtctaat tcgccttttt ccaaatagat acttcccaat acagaatagc
     5761 attcagcaac tcttttatgg ttttctccaa atttcttttt attaatctct aacgcatttt
     5821 ttactaaatg tatggcttcc tctgttctgt tttgttcaaa ataaattaaa gataaattgt
     5881 aatttactct agcaatttca gttgcattgt gatgtggaaa tttttgaaac agctctttgg
     5941 ctttcaaata atactgcatt gccttttcaa tctccgattg ataataataa acaacggcaa
     6001 tgccattggt tgccattcca cattttctga tatattttga ctccttaaat ttagaaagca
     6061 aattgattgc atttgtgaag tttgattttg ctaattcaaa ttgattttta cgcaaataaa
     6121 tagttcccag aagaaatgct ttagagcctt tattgaaaat tgttttaggc gtatattgtt
     6181 cagcaaaaaa atgtgctttt tctaaattat ttgtatttaa tagatattgt aattgcaaca
     6241 aatctgcttg ctcaattaga ctagaatctc gaatagattt tctaaatgca gtcaattctt
     6301 gctcgaaagt ctcaaagttt tgcaacataa aattacattc aagaattttt aaccttgaat
     6361 tttcattttg taccgcttgt tgaaacattt ttttcgcctg ctgataagct tcaagttcaa
     6421 acagttgaac gcctttctca aagctcacat ctttagaaaa agatattaga aaaataaaaa
     6481 ctaaactaat cgataataat ttcttcagca ttcgtataaa aatacatttt atatttatat
     6541 catatacctt ttaaaaacaa taaattatct ttgtagttat ggaaaagaaa attgcagttt
     6601 tgccaggcga tggaatcggc ccagaaatta ttacagaagc agtaaaagta ttaaatgcta
     6661 tagcacaaaa gtttggacat caatttcaat atgaatatgg attaattggt gctgatgcta
     6721 ttgacaaaac aggcaatcca tatccagatg caacgcacga actttgttta aatgctgatg
     6781 ctattttatt tggtgctatt ggcgatccaa aatatgataa tgacccaagt gcaaaagttc
     6841 gccctgaaca aggtttgcta aaaatgcgtg gtgctttagg cttgtatgct aatattcgcc
     6901 cagttaaaag ttatgatgcg ttattagatg catctccatt aaaaaatgat agaataaaag
     6961 gtgctgattt tgttgtggta agagaattaa tttcgggaat ttattttgga aaaccacgag
     7021 gcagaagtga agaccaaaat atcgcttacg atacttgtgt ttatactaga gaggagattt
     7081 tacgtatcac tgaaaaggca tttgaatttg caaaaaacag aaataaaaaa gttacacttt
     7141 tagataaagc aaatgtttta gccacatcaa gattatggag agaagttgtt actgaatatg
     7201 ctaaacaatt tcctgaaatc actttagaat gtaattttat tgatagtgca gcgatggaag
     7261 tgattacaaa gccaacaaaa tttgatgtta ttctgacaga aaatcttttt ggtgatatta
     7321 tttcagatga agcctccgta attggtggaa gtttaggctt gttgccttcg gcatcaatgg
     7381 gcgagaaaac ttccttgtat gaaccaattc acggttccta tccacaagca aaaggtaaaa
     7441 atatagctaa tccaatggca acgattttat ctgctgcaat gttgttggaa tatagctttg
     7501 gattaattga agaaagcaaa gcaatcgtaa atgctgttga taaagcattg aatgaaggat
     7561 ttgttacgga agacatcaat caagggaaat acaaaaccaa cgaagttggc gataaaattg
     7621 ttgaatttat tttgcaataa ataagtatga aaattgccat cataggtcct gcttatcctt
     7681 atcgtggtgg aattgcaaat ttttcagaac gattggccta tgaattgcaa ttggaacaag
     7741 agaaaattca attgtttaca ttcaatttga tgtatccaag ttttttattt ccgggtaaat
     7801 cacaatatgc agaaagtgtt ttgcagtttc cgttggaaat aacacagaat attaattcta
     7861 ttaatccttt taattggtgg aaagttggaa aatatatcca aaaacaaaat tttgatttgg
     7921 taattattcc ttattctata ccatttttgg caccaagctt aggatttatt gcaagagtga
     7981 ttaaaaagaa cagccataca aagattttgt ctattattca taatctgcac ccacacgaaa
     8041 aaagatttgg cgataagttt ttatctaatt tttttctaaa aagttgtgat gtgtttttga
     8101 ctttatcttc aaaagtaaaa gaagatattt taggcattcg ttcaaatgct aaagtaattg
     8161 tttccaatca cccaatttat gatacatttg gtgctataat agaaaagcaa aaagcaaaaa
     8221 aacaattaaa tttagaggaa agcaagcaat atattttgtt ttttggctta gttcgtgcat
     8281 ataaaggctt agatatttta atagatgcaa tgtctattgt ggctcaacaa aatccaaata
     8341 ttcaattgct aattgctgga gaattttatg gtgattatcc aaaatacctt gctcaaattg
     8401 aagctttaaa tttatctcaa cgtattgttg taatcaatca atttattcca gaagataaag
     8461 taaatagtta tttctgtgct gcagatattg ttgtgcagcc ctataaaaat gctacacaaa
     8521 gtggtatcac acaaatttgc tatcatttta ataaaccaat gatagtaaca aatgtgggtg
     8581 ctttggcgga aaatgtagcc aataatcatg taggatttgt ttgtgaacca agcaaagatg
     8641 ccgttgctgc atctatttta aagttttatc aagaagaaaa agaagctatc tttattgaaa
     8701 atattaaaga agaaaaaaag aaatacagtt ggcataaatt aagtcagcag attaaatcag
     8761 ttttccaata aattatgcaa gagtatatca ttcaaatatt aaaagagacc atagagcaaa
     8821 gaaataaatt gctaaacgat gctgtattac tcggaaatat tgaaaatatt gcacaattac
     8881 ttattcaaac ttttagacag cataataaag ttttgttttg tggaaatggt ggttctgctg
     8941 ctgatgcaca gcatttagcc gcagaatttt ctaataagtt tatgaaagat agaaaatcat
     9001 tagatgctga agcattgcac gttaatactt cttatttaac ggcggttgcc aatgattatt
     9061 cctatcgaga tgtgtatgcc agactaattg aagcaagagg gcaagaaaaa gatgttttga
     9121 tagccctttc tacttcagga aattcagaga atattgtgga ggctgtaaaa atggcaaaac
     9181 aaaagcaaat ggttgtggtt ggttggacag ggcaaaatgc tggcaatatg gataattatt
     9241 gtgacatttt aatcaaggtt ccgtcagata atacagcaag aattcaagaa atgcatatca
     9301 ccattggaca tattatctgt caattagtag aagcagaatt attttaattt ttttggcata
     9361 gctattgcat ttatcaacat aaatgtttaa ctttatctat taattgtgca aaagcctatg
     9421 aaaaaactat ttacattaat ttttattttt atttctttca atatttttgt aagtcaaaat
     9481 gctattgcaa aaagtttatt tgctccagat ttgaaagaaa ttcttagtat ttatcctaat
     9541 ccaattgcaa cgaatgcaac aattaaaatc tctaatgata ttaatttaca ggaaagcaat
     9601 gtgagtgttg ttttttataa tattgttgga aaagagattt ttaaaatctc tcaaattaaa
     9661 aactacgaaa tcaatatttc tagagataat tttaaaacca gcggacttta tttttatcaa
     9721 ttaaatattg atgaaaaact tcaaagcaca ggaaaagtta ttgtaaaata attttttttg
     9781 ttttcttaaa tcttcaatct atatttgcta ttgcatacta ttgtagtcgg attaaatttc
     9841 tcatccaagc ttctggaatt tctccatctt ttgcaattct gtaatcatta taaatacaag
     9901 gaattaagtg ggcaacggtt ctatctcctc tgtttacatt gattttcatc caccatcttt
     9961 cggttttttt actttttaaa aaagtaaatg cttcttcttc tacatacact gtaaaatggt
    10021 aaaattcttg ttcgtttaaa attgggtaat ccattcttct caaattcaca ccctcgataa
    10081 aataccaaat catttgggct acttgatgtg ctgtaacatt tttaatatct tccattggat
    10141 tataattgta aattccaaaa gaactcaaaa tatcactcat accagcaaat cttgccaaca
    10201 tacaagcttc atctccaaac agaccatttg gcgatggtgc aacttgacct ggagcatcgg
    10261 agaaacgaat tgcttgaata tcaaatacgg ctaaatttgc ttcacgaata aaaggctcag
    10321 cctcaaaaac attttctcga attttgccaa cacgcaaaga atcaaattga taattgtgta
    10381 ctattgtgct gatgtctttc tgtatgaaat aagattgata agcaattaaa ttgtattttt
    10441 ttagataatt agtattgaaa atattgtata aatagctttc ttctaatttc ccttcctctg
    10501 gattggcagt catatttgca ccaatctgac tgatttgtat ttcgttttct aaaatttcat
    10561 aggcattata ttgtgcttgc aataaatcta aatctccacc tataattatc aaaataatat
    10621 tattgcgaat ggcatttttg gtaacatctt ttaaagctac taaagtatct tgacgagtat
    10681 tgcctttttt gatatttcct aaatctatta ttttggtatc attgtaaaat gaagtataag
    10741 tatatagttg ttgcctaact tggttgggtg ctaattctgt tccttgattg tcatcattat
    10801 tcaaattatc ttcaacacca actaatgcaa ttttataagg ctcaatttct gttgtatcta
    10861 aaccaaaaaa tgccacatta tttggtaaat tcggatttcg agtgatacat tcattataaa
    10921 attcctgagt aattggttct acaaaatcaa acatcttttt tatttattgg aataaaagta
    10981 gaaaatgccc atgaaattag tcaaaatatt tactcaacta atgtgtatta cttttttcca
    11041 taagttacgt aataatcgaa aataaattcc ttttttataa gaattgatgg caatagatta
    11101 attttgtttt gtgccataaa gccttcatat tctttcaaat tatattgatg tggatgcaaa
    11161 acatcacctg gcaaaatttt gaaaagctta tgtaaaaatg catttctgtg tgcatcaata
    11221 gatacaatta gccaaccatt tggctttaaa gcttctgcta attttttata ggctaattca
    11281 atgttttcta catggttaat cgcattcaaa cagaaaataa catcatattc ctctttagtt
    11341 tcaatttgtt caatactttg cgttataaat tgtgtgtacg gataccaaac tgggtcaaaa
    11401 tgtgctaatt ttttatactc atctaataaa ggatcgagtg catagacttt attatttttg
    11461 agggcagtaa aaatacctgc gggtccacaa ccagcatcta aaatagttaa gttttctagc
    11521 gttggaatgt ttggaatttt agtaataaaa tctttccaat attgttgttt ccacttcaga
    11581 taagtttgga tatctttatt gtctaaataa tttttccacc attttatttc taaagattgt
    11641 gctaatttcc atttccaatt tcctatcata agctttgtaa ttatcttcgt ggtttatact
    11701 atcaaaatta tagaatttac gatattttta aattaaaaag ttgctttgca ttttgagttg
    11761 tttgtgtcgc aatttcctct attgtttttt gataaatagt ggctaaagtt tcggctacta
    11821 tttttgtata ggcactttca tttcttttac ctcgataagg aactggcgga agataagggg
    11881 catcagtttc caatacaata ttttctaatg aaatgttggt taatgtttct cgaagatgtg
    11941 tgtttttata ggttacgaca ccgccaatgc ccaaataaaa tcctaatttt ataatctcat
    12001 ttgcttcttc caaactgcct gtaaaacaat gaaaaatacc ttttggattt tgctttctct
    12061 tctttaaaat atctaagatg atataggtgc tttctcttga atgaatagca attggtagct
    12121 gatgttccaa tgcccaatca cattgagttt caaacgcctc cttttgtatt tctaaagtag
    12181 ttttgtccca atataaatct attccaatct caccaattgc aataggtttt atgtctgcta
    12241 ttctatcata aattgttgct agttcttgtt gataattctc aggttttaca taacaaggat
    12301 gaagtcccaa catcgggaag atttgttgtg gatattgtcg gcaaattttc atcattggct
    12361 ctatagttga aacatcaaca tttggaatcc acatattttg tatgcctgca tcaaatgcac
    12421 gttgtatcat ttcgtcccta tccagattaa aattgtcttc gtataaatgg gtgtgcgtat
    12481 caatcaaaaa catattctgc aaaagtaagt attttagtat agtctgaaag gtaaagaatg
    12541 atgaaattaa ttctattcta tctattatcc ttaaattgat gcgtgttttt tgctaaattt
    12601 atacaatgat gaatgaagta cagtatttta agcatagcag tgccattata gatgacggag
    12661 caattattgg aaaggatacc aaaatttggc atttctgtca tattatgcaa gcaaatattg
    12721 ggagcaactg tatattagga caaaatgttt ttgttgcaaa caatgtaaaa atcggcaaca
    12781 atgtaaaaat ccaaaataat gtttcagtat ttgaaggtgt tactattgaa gatgatgtct
    12841 ttattggacc aagtgtcgta tttacgaatg tcaataatcc aagaagcttt attgaacgaa
    12901 aagaggaata caagcataca ttagtcaaaa aaggagcgac aatcggagca aacgcaacaa
    12961 ttgtttgtgg ttataccatt ggagaatatg cttttattgg tgctggttcg gtagtaacta
    13021 gaaatatcaa taattttgaa ttagtaattg ggaatccagc acgtcagaaa tattgggttt
    13081 gtaaatgtgc tgaaccctta cattttgaaa ataatgaagc attttgtgct aaagagcaaa
    13141 agacgtatat tctcgaaagt gatattgtta cgataaaaga ataaggaaca acaagcacaa
    13201 agtataaaca aggcactcaa atattttaat atttcatcgt tagagaatcg gctgttttca
    13261 agtttgtcat ttggagaaca acgtttagtt ttatttatca gagctattat aaaattacca
    13321 aaactactta ttttagatga agcttatcaa ggctttgacc aaaacaccat ttcaaggtgc
    13381 aatacttata ttgacgaagt actgccaaaa tcaaccacgc ttatttttac ttcacactat
    13441 ttagaagaaa tacctaaatg tattagtaat ttcttatttt taagcaatgg aaaaattgtc
    13501 tccaaaaatt aaatttgcta tcattggagt tgctggattt gttgctcctc gacatctcca
    13561 agctataaaa aatatagacg gagatttgtt agcagctttt gatatttcag actctgttgg
    13621 tgtgctagat agttattttc cagattgcga tttctttact tctattgata catttgaaaa
    13681 atatattaaa caaacaaaaa tagattattt ggtcgtttgt tctccaaatc atttgcatat
    13741 agaacattgt cttgtgggtt taagaaatgg cataaatgtt atttgtgaaa agccattgtg
    13801 tctaaattct gaggatttaa gaaaattaca ggaagccgag caagcttcac aaaagcaaat
    13861 atttacaatt ttacagctaa gattacatca tgaaattatt gccctaaaag aatatataaa
    13921 tccaactgtt ttttatgcag gcgaactaaa ttattataca ccaaggggaa attggtatta
    13981 tgaaagttgg aaaggtatag aagctaaatc tggcggaata gctactaata ttggggttca
    14041 tttttttgat atgctgtgtt ggttttttgg agagctgcaa gaaattattg tattacaaaa
    14101 agatgcaatt gaagccaaag gaattttagt ttttaaaaat gcaaaaataa aatggcattt
    14161 aagtattgat agaaagctag tagcaaaaag gcagattgtt ctgaatggaa aagaaattgt
    14221 atttaccgaa gggtttaatc atttacatac aaaatgttac gaagcaatca ttgctggaag
    14281 agggtttaaa acaatagaaa tcttatcttc aacgaaaatt attgaacaaa ttcaagctta
    14341 tcattaataa gctcgaacat ttttaccttc gataaaattt acaaaagatt ttaagacaac
    14401 tttattacca cctggtgttg gataatttcc tgtaaaatac caatctccta agttttttgg
    14461 acaagcaatg tgtaaattat caatcgtttg ataaattact tctatgtctg aaaaaatatg
    14521 ttttggtcga acaatttcag cgatttcatt agaaatatct tcatctgaaa aaggagcata
    14581 aatttctttg accaaatttt gcatttcgga aataggtttt tctaattctg caatacattt
    14641 cttataaatt tcttggacaa cagatgcttt gttacttttt ttccataggt ttatggctgc
    14701 ttggaaagca acaaatgtac ccattttact catatcgata ccataacaat caggatatcg
    14761 aatttgtggt gcagacgaaa caataattac ttttttaggt tgtaatctat ccaacatggt
    14821 caaaatactt tctttcaaag tagtgccacg aacaatagaa tcatcaatga ctactaatgt
    14881 atctacatta ttgcttacca atccgtaagt aacatcataa acgtgatgta ccatatcatt
    14941 tctagaatta tcatctgtaa taaatgttct cagttttaca tcttttacag caattttttc
    15001 tactcttggc ataatcttta aatttgcctg tagttcgttg aaatctatag tttgtttgtt
    15061 gtataaatct aaattatact cgatatgctt tttttgaatc tcttctacca tacccaaaaa
    15121 agcgacttct gctgtatttg gaatataaga gaaaacagta tcttttagat tgtattgaat
    15181 ccgttctaaa attttaggaa ctaataattt gcctagattt ttacgctctt ggtaaatttc
    15241 ataatcatta cctcttgaaa aataaattct ctcaaaacta caagatttct tttctcgtgg
    15301 aggactgcaa agcacttcac cacaactgcc atctttctta atgattaatg catgaccagg
    15361 ttgtagttct ttaattttac ttctgtgaac attgaaagtt gtaataatgg cgggtctctc
    15421 agaagcaacg accaccactt catcatcttt ataataatat gcaggacgga tgccatttgg
    15481 atctcttaat gcaaaagcat cgccatgccc aatcattcca gccattgcat agcctccatc
    15541 aaaatcatca gttgcatttt ttagaatctt ttctacattc atattttctg caatcaatgg
    15601 cgtaatatct acattctgta aattgcgttc tgatttgtaa gttctaaata atttttgatt
    15661 ttcagaatct aagaagtgtc cgattttttc taaaacagta atcgtatcag aagcttccct
    15721 agggaattgt cctaaactga caagtttctc aaataattca tcaacattgg tcatgttgaa
    15781 atttccagca acaatcaatg ttctagtctt ccaattgtta gcccgaaccc ttggatggca
    15841 attgctcatg tcatttccgc cgtgtgtacc atatctaaga tgacccatca ataattcgcc
    15901 catgaaagca taattttgct tcacatagtc catgtctttt atggcaatct tttctgcttc
    15961 tgagatatta ttgaattgct taaatacttt accaaatatt tctttaatag catcttttgc
    16021 gacacttctt tctctatcaa agtaaggagc acctggtttg gtatctagtt ttatggttgc
    16081 aattccagca ccatcttgac ctcgattgtg ctgtttctcc atcagtaaat acattttgct
    16141 taatccataa tgtatatcac cgtatttttc tcgatagtat tctaatggct ttaataacct
    16201 gattaaagca ataccacatt catgtttgat aaaatcactc attcggaatt acaaaagtac
    16261 tgcttaattt ccgaaaaatc attttaaatc aatgtttaaa attttttaag agaataaatt
    16321 tattttaagc tgatagagtt gcctttggtt tatatattct ttttttgaaa tatcgaatta
    16381 agaaaaacag aaagacaaaa agtacgatgc aaattattgc agcaataata aaaggcataa
    16441 aaatacttaa aatacttcct gcaaatgaca aaccatgttc agtagttgca acaacaggat
    16501 taccaacacc agcagtggct acgctcgaag ttgctctggt taatgctgtt ccagcttgta
    16561 caatgcctgc acttcctccg cctacaatta ttcctaagcc ccattttaac ataggatcta
    16621 attctggtgt taaaacagag gcggtcaata aagtacctgc tatcacggaa gttggtgttg
    16681 taatagtgtc taaaatatta tcaacaaatg gaatatagta accgccaatt tccaaaactg
    16741 ttgcagttgc gaatgcaaca atggcagttg tgcttcccat ccattgaaaa ctttcgccca
    16801 aatgaataat gcccaatttt gatgcaatac ttgccaccaa aagaggaaca aatactctga
    16861 aaccactact tgcactcaat cctaagccta atgctaagct gagcaatagt ttagattgtg
    16921 gcagtaagtt gggtatgttt tcagaaatga tgttgctaat atttggtaat tccattttag
    16981 ctatttttgt tttattgatt aaatatcaaa tttgttgcca aattctacat taaatctaca
    17041 tctttttgaa taaaaacatt tttaaatttt tcaaattgat atagattctt tatttcataa
    17101 tctatcattt gtttgatatg actaagttga gtaaaatgtt ctcttggaat ttttattaat
    17161 atttctctaa tgaaatagtt atttattctt tgaaaaatgg gcttgtttgg tccttttaac
    17221 cattcgttgt aatgtttttg taattgctta cttagtaatt gggctgcttc ttcaacaata
    17281 cttaaatctt tatgtcgaat ggttatgcca attaatctgg aataaggagg ataggaaaaa
    17341 agctttcttt ctgatatttg cgaaagataa aaatctttaa aatcattctt caatacagct
    17401 tgaacaattt ggtgttcctc attagaaatc tgaatttcta cttgtccttt tacatctttt
    17461 cgtcctacac gaccacttac ttgaatcagt aattgataag ctcgttctat tgccctaaaa
    17521 tctggaaaat agagcaaggc atcggcattt agtacgccta ctaattgcaa atttttaaaa
    17581 tccaatcctt tggttaccat ttgggttccc actaaaatat cataaacacc atcttcaaaa
    17641 gcatgaataa ttttctcgtg tccatattta gtttttacag tatcataatc cattctgcca
    17701 attttagcat caggaaacaa actctttaaa tcatcttcaa tacgctctgt cccaatgcct
    17761 ttttgttcta aattattatc accacaagct ttgcaatgac taatatgtgt ttcagtatag
    17821 ccacaataat gacaccttaa atcattggtg tatttatgat aggtcagtgt aacatcacaa
    17881 tttttgcaca ttggaatcca atcacaagat ttgcaactta aataaggtgc ataacctcgc
    17941 ctattttgaa ataaaacaac ttgttttttt tcgtttaagg tatcttgaat tttatctcta
    18001 agttcaaaag taattccact aacaaatttt ttctcttttt gtgcattttt ataattgatg
    18061 aattttattt caggtaatgg cacatcatta taccttttag ttaattcgac tagttgatac
    18121 ttattttgtc gagcattaaa atacgactca aaacttggag tagcagaacc taaaattaca
    18181 ttagccttat gcatatttgc caacatcaga gcagcatctc tagcatgata acgaggatta
    18241 ggttcttgtt gtttatagga agcatcgtgt tcctcatcta caataattaa acctaaattt
    18301 gaaaatggca aaagcaatgc cgaccttgca ccaacaataa tttgtatttc ttgcttcaat
    18361 actttgttcc aaatttctac acgctcacta ttcgataatt tagagtgata aacagcaata
    18421 ttacccaacc agttttgtaa tctttgaatt aattgtgccg tcaatgcaat ttcaggtaaa
    18481 agaaataaaa tctgttgatt ttggtgcttt aaatttttaa taatttgagt ataaactaag
    18541 gtttttccac ttgatgtaat gccatgtaat aatacaggct tttgaacttg aaaagcttca
    18601 tctatttgct gtatcgcctt attttgttct tcatttagtg agaattgatt tttatgttct
    18661 gatttatcaa tggtgattct atcaacttcc tcttcataga tttcaaaaat tcttttttcg
    18721 actaatgtat ttaatgttgc agaagttgca tctactcgtt ttaataaatc acttcgtttg
    18781 atgcgtttat cttttcgttc taaatctata aacccaagaa gaatttgttc ttgtttggga
    18841 gcatttttta ctaattcaaa gcaacttgcc ttctgattgt tgtcaataat atcttgatgc
    18901 catcgtacaa aaatttcttt tttgatttga tacttttcat ataagatttc ttcaatgaaa
    18961 atcaatcgat taaaaattaa ttctttaata gttttataga cgtgcttctt tttgacaatc
    19021 aactgaatgt ctttaagcga taagcttggt tgaaattcaa atgctttgca aataatttga
    19081 gcatcttcag aaagatgtaa ttcattataa tcaattaaat catttttgat ataaatactc
    19141 tcactatcta attttaaata agatggtaaa gacgcattca taatttcgcc caatgtacac
    19201 atataatagg aggacatcca ttcccaaaat tgcaattgtt tttctgtgat aacgggttct
    19261 tcatcaagca ctgaaagtat tggttttgct tgataatctt taggtggatt ttgatggatt
    19321 ttacaaatta gtgcagaata gattttatgt ttcccaaatt ggatttctac acgtttgcca
    19381 attcctaact cttgttctaa ttcttttggc acaccataag tatattgttt tggcaatgcc
    19441 aaaggcaata ttacttccac aaaaatatca tatttttcaa aaatatccac ttcacaacaa
    19501 aggtgtaaaa tttatttcag aaagactaag tctaaacaac tattttttta cgttgtaaag
    19561 gaagataaat tctattgata ttgcaatatc tatagatacc tatattattt acagaaaaac
    19621 ttaaatgctt ctgttttttt attcttttga attctaactt gataagctat ttcatctttc
    19681 aatagaaaac ttacatttaa aatgccatat ttatttggtt caaagaagta atagtaatat
    19741 ttaagcttca tgccctcttc tacttttgtt tcctgatcgc tacgcatgat aggtatatca
    19801 atatattttt cgctaatttc aggcatattt ctaaaaatta taaaatattt acgcacatta
    19861 atttctaatt ttttattaag tagtatgtca ttagcattat ctttttcttt gtttatagta
    19921 ctgtatgtgc aatcgtaatt aaagcttgca tcccaagaag gaagtgatga aagcttattc
    19981 gctttcatat tttcattaaa atttttaata ttttcttttt gaatactgtc agtaataaat
    20041 atatttttaa ttgtaatagc taataagttt ccatcttcca cacttatttc attggcataa
    20101 tctagtgcat ttttaccatt ctcatctttc ttcatcaatt cataaggatc tggatctaaa
    20161 ttaccttgca tgtgcttata ttttactgga acaacttcta tgccttttaa attaacaaaa
    20221 cctcggttag tatcaatggc tactgttgct aaattatcat aataactaga gatttgatca
    20281 tatttaatag ggataaatag tttggtagta gccgtatcaa taagtcccat tttagctttt
    20341 gccgaattga tagctataac ttttataaat ttatcttctc tttcaccatc taattttatt
    20401 tcatcatatt gaggtgtaat taactcttta tgtagtttta agctaaatag tccatattta
    20461 ctatctaatt ctacatttac taaatctata atatcatcgg aatagttaaa aaagttttca
    20521 tacttaaaag gaataacttc ttttccttta tcatctatta aaccccattt accatttaat
    20581 ttagctttca aaaaaggttc ttctttatat tttattatct catcataaat aacaggaata
    20641 atttctttgc ctgtttcttt atcaatacac ccaaacttat tattccgctt tgctaaacta
    20701 cttccatttt caaacgtaga aatttcatta tatattattg gaataatttc tttgcctgtt
    20761 tgttcatcta tataacctag tttgtctcct ataaaaactc gtgttcttcc aggtttttca
    20821 gtatggtaaa aagtatattg aataggtgct atcacatttc ctattgtatc aaatattccc
    20881 cattttccat ctaattttac ccatataaca gaactatcta taagaacttt atcatacttc
    20941 aaaggagcta ataattttcc tttcatgttt ataacacttt cttttccatt aagatataca
    21001 taaacgagat ttttaccgag agctcccatg tcatcatata ttggaggaac tatttcataa
    21061 cctttatcat ttatcaatcc aatttttcca tttaatatta ctttgcagaa tccttcatcg
    21121 aatacataaa ctgaatgata tttaagcgga acaacaacat ttccagcctt atctttaaaa
    21181 ccataaagcg ttttttcatt ttttttctta gtattataat ttgaaaatgg aaataagtct
    21241 tctgctgtag aaatatgagc tatgcttaat agtataagta gtaaataaat tgttttcatt
    21301 attatagatt tgttttcaaa tatacttatt ccctctaaaa aaaattgctt tgttgtatat
    21361 taaaatgtgc taaatctatg taagagttca attacaaaac atttgatatt tcaaatctct
    21421 ttactaaata gaaatattac tatcattaag caattccgtt atattttcgg attatccatt
    21481 cggcagaaag caataaaaga ataattccaa acaatatttt ccaatctatc aatggttgag
    21541 tttttgtatt ttcaaataat atagattgaa tgttttcatc tttctgaata gcatcagcga
    21601 tactttcaag attttgtaca gtataagttt ttccaccact ttgttgagct aaagttcgca
    21661 atattgacca attagcttgc aaattttgta cttcaatatt tatgggctga actgagaatt
    21721 tggtggaggc tttaaattgt tgtcctttgt aatttgtttg tgcattagct gtgtaatctc
    21781 cagcaggtaa aattccagta tttaaaatat atgcattaag cgttttgtca aaaacaaaac
    21841 gataagcatt ttttccgttt ttaagttcta agtttacttc aggttcattg attagtacaa
    21901 aactttcgtt gtataattga gcttctaaaa cgataggttc atttgtgcca taaatttgtt
    21961 tcgaggtact tattttgaat tttcgtttgt cagtttttac cgtaagaaat tgaatagatt
    22021 tgttgataag aatatcagtt gcatcagtat ttttaaattg cataaactca ttcaaacgcc
    22081 aacgccacca gccttcacca gcaaaaatac ctgttttttt attattttca tctgcaaata
    22141 aaattaatgg attatttgtc ggcactttgc ctaattgttg cctaaataag atatcagcat
    22201 ttggattgac agaatatttt ccataaggtg ttaaaattgg tgggagttgc tttaatatat
    22261 tggtgctttg ctcatctaaa acaaatttag aaaagttact ttgtggaata ggaaaaacat
    22321 catttccatt cagagaactg cctgatattt ttaataaatt ctgtaaatta ttgaatgtgt
    22381 ttatattcgc ttgagcggat agaatatata aaataggcaa cttactttgg ctgatttctt
    22441 gtattaaatt tgcatctaaa ctagaactta aatgtaagat aatcatatca tatttgtcca
    22501 actttttatt aaagttgtca gcactttcta cttctatttg atagtttttg ttgctttcaa
    22561 tggcatattt aaaggctttt acatcgggat gaggagagcc ataaagtaat aagatttttt
    22621 gtcttccatc tataacttca atataagcag tttgaatatt attttcactt atggtttcgc
    22681 catctatcat ttttaatcga acgtgatatt taagaattcc gggcttgtcc gcttgtaaaa
    22741 cgatttgtgc agaggtacta aagtcatctt gatggataga gattgattta ttgaatacta
    22801 tttttccatt actatcaata acttctaaaa ttgtgttttt cccattcaat aaatgtgcat
    22861 caacatcaac ctgtagattg aaattatcag ttaaataaac aatattgggg tataataaat
    22921 ttctaattag tgcatctttt ttgatagttg tatcgcccaa agcaatagta taaataggtg
    22981 ttttggtagc aaaaggatta taaactggat tattacctac attataaatt ccatcagatg
    23041 ccaaaataat agcaccaata ttgagatggt catattttaa gaaagtttgc ttgatggcat
    23101 tgtcaatatc agtactttgt tgcgtaaaat ccaatgtttt attagtagat aagtttcccc
    23161 caaattgatg tgtaataaca ttgtattttt tacccaattg tgttgttaat gcattgagtt
    23221 tttcttgata ttgctttaaa ttgctttgtt taaaaacatt ttttaatgaa gcagaattgt
    23281 cttgtaataa aacaattgta ggtttttgtt cttctgtttg agttgttttt attaatggag
    23341 aaagtagtaa gaatgctatc aaggaaacag ccagaaagcg aagcgttgct aaaatatatc
    23401 ggagtgattt tttttcttca gcaaagaaga tgaaagattt tctgccgtat aataaatatg
    23461 aaaataaaaa tcctaccagc aaacaaattg caaagaacca aatcggatat tcaacaatta
    23521 gtttcagata aaatttattt taaatgataa cagtcttatt tcagcacttc tgccaatgtt
    23581 tttcctaaaa atgctggtga ttcaacaacg tggataccac attcgcccat aattttcatt
    23641 tttgcagcag cagtatcatt cgcacctccg ataattgcac cggcatgtcc cattcttcta
    23701 ccttttggag ctgtttgtcc tgcaataaaa ccaactacag gttttttgaa attatctttt
    23761 atccaataag cagcatcaac ttccatagtt ccaccaattt caccaataat gacaattgca
    23821 tcagtttcat tatcgttagc aaattgttca acagcgtctt tcattgttgt tccaataatt
    23881 gggtcgccac caattcctat tgcagtagaa attccataac cagcttttac cacttggtca
    23941 gcagcttcat aagttaatgt cccagatttt gaaacgatac caactcttcc ttttttaaat
    24001 acaaaacctg gcataatacc aactttgcac tcttcagatg taataattcc aggacagttt
    24061 ggtcctatca ttctgacacc tttttgtttt acataagctt ttgctttaat catatcttgg
    24121 gcaggtattc cttcggtaat cgtaacgatt agttgaatac cagcatctgc cgcttccatt
    24181 attgcatcag cagcaaaagc aggcggaaca aaaataattg aaacattagc accagtttgt
    24241 tttactgcat tttctactgt attaaatact ggtctgtcta aatgactttg tccttctttt
    24301 cctggagtaa caccaccgac tacatttgtt ccatattgaa tcatttgttc tgcgtggaat
    24361 gtgccttctt ttcctgtgaa tccttgtacg attactttgg agtctttatt aactaagatg
    24421 ctcatatttg tttttttaat ttatctaatc taacatttct tgcttctttt aagaaatctg
    24481 aagcaaaaat aaaatctaat aattctttgt tttcagcttc aaacaaggaa actttatcgc
    24541 cttcccaatg tttattgcct ttatataaaa aaagtatttt ttcgccatgg gttacaacag
    24601 aattcatatc gtgcgtatta attactgttg ttatattata ttcttcagta atctctttga
    24661 ttaattcatc tacgacaatt gaagtttgtg ggtctaagcc tgaattaggt tcatcacaaa
    24721 aaaggtattt tggattcatc acaatagcac gtgcaatacc agttcttttt tgcattccac
    24781 ctgaaatctg agaaggaaat tttttattag cacctactaa atttacacgt tttaatactt
    24841 catttactct atcttgcttc tctgatttag acattttggt aaacatctcc aaaggaaaca
    24901 taacattttc ttctactgtc aatgaatcaa ataaggcact tccttggaaa agcattccca
    24961 attgttgtct gattttattt ttttcatcta aatccataga tacataatcc tgaccatcaa
    25021 aaaggacatg gccttctgtt gggttgagta agccaactac acactttaac attactgttt
    25081 ttccagaacc tgattgtcct ataattaaat tagtttttcc aggtaaaaaa gtggttgtaa
    25141 ttcctttgag tacctgttgg tctccaaatg acttatataa atcttttact tcaatcattt
    25201 attataataa taaccacgct aatattaaat cagcaaataa aaccataaca caactcaata
    25261 ccactgcagt agtacttgct ttaccaattt ctaacgcact tccatgagca taaaatcctt
    25321 gataacaaga tattgaagtt aataaaaatc caaaaactgc tgcttttact aacattacta
    25381 caatattttt agcttcaaat gtcat
//
LOCUS       DAOQCW010000022        25031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_739_length_25031_cov_6.107383, whole genome shotgun sequence.
ACCESSION   DAOQCW010000022 DAOQCW010000000
VERSION     DAOQCW010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 25031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25031
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_739_length_25031_cov_6.107383"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2503)
                     /locus_tag="PLS16_03385"
     CDS             complement(<1..2503)
                     /locus_tag="PLS16_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ60154.1"
                     /translation="MNIKNLFFLIAFVLAFSFTGNAQSEGTVDTEDESNKTKTTVSDP
                     GTNMDIPVITLDESELGGTSMGDQNVSSILGASRDPFFSIATFGWSFARFKMRGYDSQ
                     YWENYMNGAPIDYIDNGFGAYSLWSGLNDVTRNRTNYIGIKSNPLTFGALGGTYAIDT
                     RASKQRKQLSVATAFSNRTYTNRMMITYGSGITKKGWAFAASLTGRLAKEGYVDGTNY
                     QSLSYFLSIEKVMAKNSISLTAFGAPTKNGKTTPVEQQFYDLAGSHYYNPNWGWQDGK
                     KRNAREGYRHQPVINLMHEWKPSDSKNLTTALSYSFGENSTSGLDWYNAPDPRPQYYK
                     NLPTYSSDPVQQALIAEMYRNNPDLLQVQWDKLYESNRSHVDPEFGSGRSVYALKQDV
                     KKIQRVNLNTVYNQTIKDNTELTAGLNYQFMMSKNYAQMKDLLGGDFWVDINRFAQFN
                     FPKDAVENQNNADSPDRKIKVGDKYEYNVNLYYNKTGVWGQLVHHFNKLDVFVAAEYS
                     NSTYWRKGNYRSGLFMNNSLGKSKTLSFNNYGVKGGLTYKINGKNYLFANGFVQTQAP
                     MLENVFVSFRTRDYTIDKPQNEKIYSVEGGYVLNSPKVRLKATGFYTKINDGTDLLRY
                     YDELVYNFVNVSIQNIDKTYYGGEIGMEASIYKGFGMTAGASIGKYTYSSRWNTTTYV
                     DNTQEVILPNNTVYTKNYYIGGTPQQAYSLGLNYRSPKYWFITSTVNFVDRTFTEISP
                     SRRTAQAVDLVDYKSDLWNSIVSQERINPKGVWTLDIFGGYSWRLKNQFKGLKKEQYL
                     VLNVGINNITNNKKAQITAFEQLRFDVDGKDLNLFK"
     gene            2635..3684
                     /locus_tag="PLS16_03390"
     CDS             2635..3684
                     /locus_tag="PLS16_03390"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase"
                     /protein_id="HNJ60155.1"
                     /translation="MKKYIFTFSFLCSILLGNAQNSENQYRLIVAGFYNLENLFDTID
                     DPIKYDEEFTPSGGYRYNTTIYKDKLGRLSDVLSQIGTDVSPDGLAFLGTAEIENVDV
                     LLDLVAMPKLASRNYKPIWYEGGDARGIDCAFLYNPKYFKPIYSKSIPINLNQVVKDG
                     KPTRDVLWVRGILTGIDTVDVFVGHWPSRRGGETLTAPMRNYVAGTIRHYADSIRAIN
                     KEAKILVMGDLNDNPTDPSLVKYLDAVGTKDKMTTEKFFNPFVEFYKKGYGTLAHNDA
                     WGLFDQIILSNGWMNTKQDGWFYQNAHIFKKDFMVQKTGKYKGYPKRTWNFSEYNYGY
                     SDHFPTYIQLLQKVK"
     assembly_gap    3788..3887
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3888..5740)
                     /locus_tag="PLS16_03395"
     CDS             complement(<3888..5740)
                     /locus_tag="PLS16_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60156.1"
                     /translation="MVLYYSKYQKYIKFILFFSIFFGNKNSTAQQIDSIISIYDSLIV
                     IRDSLFELEEFHKKEDRKTKVENTFKYISYINYSDRGDGVQYKGIDKYKNEQNKEIVN
                     IYIKILPPFGSDEYEQLKKSQKLGNKLHLNSKEWQARQDLLFKKGEKVNPSIFADTER
                     LLWERNRYKYVDISFSQSCDDENIEIENGVDVYVFLVDKLSYTISTGYVEESFAVATY
                     INNLFGFPNMLSLRFQTPFNKLNPLRFSTAYNYFNLFGTKIDLKTDFVFDKLNRKFSV
                     GLKKNFVSLNSNWGFNFNYNFNNIKVSLNNNLRDPSSLVGAKSNEYNLWLAYAFPIKK
                     KNKASTLKFILSTKLNYTNYMDRPFIIDGNYNEQFINNSNFLFGFGFAHWEYVILKNN
                     FYIDIPEYLPKKWSLALWLGPKIDEIVGLRNNLELKANYGKSFKKVGYFYTDFKYSTF
                     VRKKKSEQMLISLAQSYITPSYKISKNVFLRQLISTKFNYGFFYPENRYFNINNLIRG
                     FYSPTLRGSKSVTASFETNLLLNKKIAMSKGMLYAFADVGWVSDNEKKLISQSYFQYG
                     VGFGLRLRSVDLGFPFIDFQFSFYPKGKDFNVNTFQFRLYESSINAITTDNL"
     gene            complement(5755..6414)
                     /locus_tag="PLS16_03400"
     CDS             complement(5755..6414)
                     /locus_tag="PLS16_03400"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNJ60157.1"
                     /translation="MEYIHFLLELLSNTKEVLENLTAQYPNFIYGILFLIIFVETGLV
                     IMPFLPGDSLLFTAGMLCAIGAGALNIWILVPLLIIAAILGDNVNYYVGKYFSTKVLA
                     LKFRGKPMVKQEWIDEVHKYFEHYGSKTIVIARFMPIIRTITPFVAGIGNMSHNVFLL
                     YNIIGAIIWVGTITIAGYALGNVKWVEDNLEKIIIGIVFVSVLPMLLKIGSATLKKIK
                     G"
     gene            complement(6420..7409)
                     /locus_tag="PLS16_03405"
     CDS             complement(6420..7409)
                     /locus_tag="PLS16_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ60158.1"
                     /translation="MYQKSFQNTDVSNKKVLITGGAGFIGSNIAEYLLLHHAKVVVLD
                     NLATGKQENIEPFFSNPNFRFIEGDIRDFDTCKNACIGIDIICHQAAIGSVPRSVKDP
                     ITTNNVNIGGFVNMLFAAKEAGIKRFVYASSSSVYGDEKTLPKKEERIGKPLSPYAVT
                     KYTNEVYADVFSKTYGMEIIGLRYFNIFGPKQDPYGEYAAVMPLFIKALLNNEAPFVN
                     GDGEQTRDFTYIHNAVQANILAMTTTNNQAFNNAYNIAVGGRFSINYMYEAIQKYLHK
                     DIKAIHREARVGDIRDSQADISKAQELLNYQPNYTFEEGLPATIAYFKSIFNY"
     gene            complement(7437..8273)
                     /locus_tag="PLS16_03410"
     CDS             complement(7437..8273)
                     /locus_tag="PLS16_03410"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HNJ60159.1"
                     /translation="MNIFERIIQDSIFKDIWYQLREHLFNEKKWYQLLTLKPIELYTT
                     KTDIEIDGAKVDLDIKGYIENIVQRRINRVKNYLTLDFLNWNDIQSSYKFKVIIKAQT
                     DYQSVGNFLNHHFANQKYDIEENEYAILLKEFKVSSLLTKLQVEVPFVIYANKWFLKK
                     EYTGNAIFTGSVMFNNPNYTIKTRNLNYTIQSKSLIIKWVDNYYHKQIIEFLNEFLVY
                     NFKEELFLAKIQAQEQLDSFQVDKNWIQGNILDLELERIAIDHDALQGIFLANGKLEV
                     NR"
     gene            8592..9446
                     /gene="nadC"
                     /locus_tag="PLS16_03415"
     CDS             8592..9446
                     /gene="nadC"
                     /locus_tag="PLS16_03415"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNJ60160.1"
                     /translation="MIQEQELHQFIEQALYEDIRNIGDLSSLSSIDRDTKGTAKLLVK
                     DEGILCGMSVAKAIAQKVDDTLQFEELIKEGSTVKFGNIAFYLEGNAISILTAERLIL
                     NCMQRMSGIATTTQKYAKAIEGTNAKVIDTRKTTPNLRFLEKYAVTVGGGFNHRFGLY
                     DMIMLKDNHIDFCGGITKAIQKVRKYLSDNNLSLKIEVETRNIDDVKEILACGGIDRI
                     MLDNYKPEDCKMAVELINKQYEVEASGGITLETIRAYAETGVDFISVGALTHSVKSLD
                     LSLKATFK"
     gene            9465..9845
                     /locus_tag="PLS16_03420"
     CDS             9465..9845
                     /locus_tag="PLS16_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNJ60161.1"
                     /translation="MSKRVIYTENAPEPIGPYSQAILVDNTLYVSGQIAIDPFIGDVV
                     RNGVEGEAIQVMKNLESILRAANMDFSNVVKCSIFLKDMQDFPKVNAVYGKYFSENPP
                     ARETVEVSCLPKNVLVEISCIAVY"
     gene            9948..11999
                     /gene="scpA"
                     /locus_tag="PLS16_03425"
     CDS             9948..11999
                     /gene="scpA"
                     /locus_tag="PLS16_03425"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF006944.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HNJ60162.1"
                     /translation="MSLTNHIPFDEIVQKSKSSAPKISAHQKYVAGIAPFLRGPYSSM
                     YVSQPWTIRQYAGFSTAEASNQFYKRNLAAGQKGLSVAFDLATHRGYDSDHERVVGDV
                     GKAGVAIDSVEDMKILFNEIPLDQISVSMTMNGAVLPILAFYIVAAEEQGVAQSLLSG
                     TIQNDILKEFMVRNTYIYPPQHSMKIISDIFEYTSKNMPKFNSISISGYHMQEAGATP
                     EIELAYTLSNGLEYIKTGLAAGLNIDDFALRLSFFWAIGMQHFEEIAKMRAARLLWSK
                     IVAEFHPRKEKSMALRTHCQTSGWSLTEQDPYNNITRTCIEAMSAVFGGTQSLHTNSF
                     DEAIALPTDFSAKIARNTQIYLQKETKICNTVDPWAGSEYVEQLTLSIAERAWALMQE
                     VESYGGMTKAIEAGIPKMRIEEAAAIKQANIDTEQEYIIGVNVFQTDEETPFEILEID
                     NTEVRAKQIERLLQIKTTRDNNAVRVALQNIEAATKSGKENLLALCIDAARKRATLGE
                     ISSAMEKEFGRYKANIKTISGVYQKQSKSNPIFEEAQKATDTFAEQNGRRPRILIAKI
                     GQDGHDRGAKVIATAFADMGFDVDLSPLFQTPEEVAQQAADNDVHIVGVSSLAAGHKT
                     LIPQLIQALKKIGRPDIKVACGGVIPKQDYDYLYNAGVIGVFGPGTVIASCALDVLKK
                     I"
     gene            12023..13783
                     /locus_tag="PLS16_03430"
     CDS             12023..13783
                     /locus_tag="PLS16_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HNJ60163.1"
                     /translation="MAYSRIFDIIQHQLQTNPLEVCLARRYDKKNWTTYSTEKVIEIA
                     NKISSGLLQLGLKKGDKIAIVSINNRPEWQFLDLACSQIGVITVPIYPTISSADYEYI
                     FNHAEVKYLFVSDKIIHRKLREVAAKITTIQAVYSFEEVDSIASLQSLYGNINQKAID
                     EVKASIDVHDVATIIYTSGTTGIPKGVMLTHNNIVSNVKDALSIITVTKNAKTLSFLP
                     LCHVFERTINYAYFAAGASIYYADGLETISENIQDIRPDYFTTVPRLLEKVFEKIVKK
                     GKTLTGVQYKLFFWALKLAENTPLEKEKSFIQKIKFSVADKLIFSKWRAALGNNIKAI
                     ICGSAPLQPRLATIFTNAGIPVLEGYGLTECSPVVSVIPLDKKCFRAGCVGKVLPSIS
                     VRLADDGEILVKGPSVMKGYYKDDDATKATISDDGWLATSDIGEFTTEKFLKITDRKK
                     DLFKTSGGKYIAPTPIENKMKESFFIENIALVGDGEKFVAAIIQPNFSELANWAKDKN
                     INFQTNHDLTENLEIKQLFKDIISNFNTCLGKVEQVKDFILVDDEWSVDNELLTPTMK
                     LRRKKIKEFYQKEINSIYNK"
     gene            13790..15553
                     /locus_tag="PLS16_03435"
     CDS             13790..15553
                     /locus_tag="PLS16_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNJ60164.1"
                     /translation="MEVKRLFDTIYYQKSKHPQQVALASLQNGEWRTLSTDDLINEAN
                     KLSLGLLQLGIQKGDKVAVVTSESRVEWNICDIAISQIGAISVPLYPTISSKDFVHIL
                     THSEAKICIASDKGLFDKINSVRSEIPGLQEIYTFDQVEGAKNYAEISNLNPNGDIAQ
                     IKAISDTIEEDELATLIYTSGTTGLPKGVMLSHKNVVSNVVTVLAELPIEAGHVSLSF
                     LPLCHIFERMVIYTYLMGGVNVYYSGIDTLAEKLGTVRPHFFTTVPRLLEKVYEKIVN
                     KGLALTGLKRKLFFWALEMADQYEYDQKPSFTFKIADKLIFSKWREALGGRVMGIVTG
                     SAACPLKIARVFSAAGVPIREGYGLTETSPVITFNRFSEGGAMLGTVGMAIKDVEVKI
                     AEDGEILAKGPNIMQGYYKEPDKTAEVFTADGWFMTGDVGTFVTNNYGNKFLKITDRK
                     KELLKTSGGKYVAPAPIESKFREDFFVEQIMVVGEGKKFVSALIVPAFPVLEEWAKEN
                     NIAFTDRQSLISNSKVLAKIQSVVDELNPNFSHIEQIKKFKLLANEWTTETTELTPTM
                     KLKRKVISAKYAKEIDEIYNN"
     gene            complement(15560..16153)
                     /locus_tag="PLS16_03440"
     CDS             complement(15560..16153)
                     /locus_tag="PLS16_03440"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNJ60165.1"
                     /translation="MLIHYPSESIVCGVDEAGRGCIAGPVVAAAVILPKGFQHPLIND
                     SKKLNEATRKALAELIKKEAKAYSVGIIDNQIIDKVNILQATYLAMHQAIAQIQPIPD
                     LLIIDGNRFKPYPEIAHQTIIKGDATYIEIAAASILAKTHRDDLMEAYSKKFPQYNWQ
                     QNKGYPTLQHKKSVWSFGYSPLHRLTFNINKQLSMFD"
     gene            complement(16174..17181)
                     /locus_tag="PLS16_03445"
     CDS             complement(16174..17181)
                     /locus_tag="PLS16_03445"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNJ60166.1"
                     /translation="MKRLFKIIVAFVTIVSLLATLKIKTIGKPFSDYDFGNYVAKSNY
                     DTTKDVQLTFLGTSGFVINYKGKKMITDPYFSNPDVFKNFVEKLQFPKLSENILPRSI
                     YENANTIVISHGHYDHCLNIENFLTKGVPTKIIAEQHITNEINSVKNNFDVQFENDMQ
                     SYHYSSDSTFRIFPLNSPHSPHFADITLFTGLYQEPLKHLPDRFWKWKLHGNYSYLID
                     ILEKDSIVYRCLFLSGNITDASVEKVRALCENKNVDIALQIFWKEKACLPGLNKVYAA
                     GKPKKIILHHWNNFFKPYDKNIEVMRDTHLDKVLKKLNNEGKNTQIMLPFSSISFDIN
                     N"
     gene            17273..18520
                     /locus_tag="PLS16_03450"
     CDS             17273..18520
                     /locus_tag="PLS16_03450"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNJ60167.1"
                     /translation="METILPLLEQVSAASVDAKRLTDVQKVAFLDTLATLLVQNTDLI
                     VAENKKDLDKMEDSNPNKDRLLLNATRIQDLANSLHDVAKLEDPTGKLLYKKTIDNGL
                     EIEKKTVPIGVVGVIYEARPNVTIDVAALCIRSGNAVVLRGGSDADFTNKILVQFIHQ
                     ALEKNQLNKEIVQLMPVDRKFVKDLLEAVQFVDIIIPRGSQQLIDFVRANSKVPTIET
                     GAGVCHTYVESTANLDKAAKIVVNAKTQRPSVCNSLDTILIDKTVAKDFLPKIIDGFN
                     QFGVEIFADEDAYAILNKANYSGLHKATEENFGMEYLSQKCSVKTVENLEEALTHIRK
                     YSSKHSEAIISEDADKIARFLNDVDAAAVYSNASTRFTDGGVFGLGAEIGISTQKLHA
                     RGPFALEKLTTEKWFVKGNGQTR"
     gene            18543..19292
                     /gene="bla"
                     /locus_tag="PLS16_03455"
     CDS             18543..19292
                     /gene="bla"
                     /locus_tag="PLS16_03455"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_119052254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B1 metallo-beta-lactamase"
                     /protein_id="HNJ60168.1"
                     /translation="MKFQLQILVFICSAFAISCGSSKKTTETRGQLYKSEDLVINQIS
                     PNAFVHTSFLQTNDFGLVPCNGLIVRDKNEVVIFDTPENDKSAEELIKWISNKLNCKI
                     IAVIPTHFHADCLGGLNTFTKYKIPSYAYFKTIEFAKENKVAVPEISFKDSLILKVGN
                     KEVIAKFFGEGHTKDNVVGYFPSEQIMFGGCLIKELNATKGYLGDANTKDWSSTVLKV
                     KSAYPNVKIIVPGHGNYGDQKLLDYTIQLFK"
     gene            complement(19285..20658)
                     /locus_tag="PLS16_03460"
     CDS             complement(19285..20658)
                     /locus_tag="PLS16_03460"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNJ60169.1"
                     /translation="MAEYKVVMPKMGESIIEATILSWHKKEGDTIAENEILLEVATDK
                     VDSDVPSPVSGTIKRILFKPNDIIPIGEALAIIETDTNVSVSTPNPETEQTEQVQKTI
                     ENQAKEEVPIVEEKNEIKATIPTQEVISIEQKNIQLPKPKEAIQYSNNYSPLVLNISK
                     QEGISATELDSIIGSGLGGRVTKEDILAYAEQKKKGNIQTISTSSIEKVNEVTTKIAP
                     KIVLGDEIVEMDRMRKLIAKNMVASKQISAHVSSFVEVDVSKIVRWRAKNKEIFKQKY
                     GFNLTYLPVFIEAIAQAIKEFPDINISVDGDNIIYKKQINIGIATALPNGNLIVPVIK
                     STDQKNILGIAQEINLLANKARTNSLTIDDISGGTYSVSNIGTFGNIIGTPIIPQPQV
                     AIMAVGVVTKKPVVVELEDEDIIAIRSMMYLSHTYDHRVVDGMLGGKFVKRVATILEN
                     FDETQAI"
     gene            20825..24142
                     /gene="secA"
                     /locus_tag="PLS16_03465"
     CDS             20825..24142
                     /gene="secA"
                     /locus_tag="PLS16_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNJ60170.1"
                     /translation="MLKFITKGLSKVFGSKSSRDIKSIMPIVEQINIEFAKLSTISND
                     ELRGKTYEFKNRIVHYLSAFDEKINELKEQAEKDDVNIEEKETIFNEIDKLTKDRDAA
                     IEEILLEILPEAFAVVKETARRFSENTTLESTATQLDKDLSVKHPHITIQGDKVIYQN
                     RWLAAGNEVVWNMVHYDVQLIGGIVLHQGKISEMATGEGKTLVSTLPAYLNALADCGV
                     HIVTVNDYLAKRDSEWNAPIFNFLGLTVDCIDKHESHSEGRQKAYRADITYGTNNEFG
                     FDYLRDNMSRTKEEQVQHKLHYAMVDEVDSVLVDDARTPLIISGQVPRGEEQMFYELK
                     PRIERIVNAQKKLVGDYLSDAKKLIANNVTGVKDGEGGLALLRAHRGMPKNTALIKYL
                     SEVGVKQILLKTENHYLQEQQKHMPFVDTGLLFVIDEKNNQIELTEKGLEMMTTSGEE
                     SDLFIMPDIGSVIADIENEETLSASEKIQKKDEAIQDYTQKAERVHTINQLLKAYCLF
                     ERDVEYVVMEDKVKIVDEQTGRIMEGRRYSDGLHQAIEAKENVKVEAASQTFATITLQ
                     NFFRMYHKLAGMTGTAETEAGEFWEIYKLDTVVIPTNRTIARDDREDLIYKTKKEKYN
                     AIIDEISKLKAQGRPVLLGTTNVEVSELLSKLLTMRKIEHNVLNAKQHQREADIVAEA
                     GRAGQITIATNMAGRGTDIKLTDEVKAAGGLAIIGSERHDSRRVDRQLRGRAGRQGDP
                     GSSQFYISLEDDLMRIFGSEKLSGMMDKMGFKEGDVIQHSWVSGSVERAQKKVEENNF
                     GIRKRLLEYDDVMNAQREVIYKRRNHALAGERISFDLMNMFFDLAEETVENAKQNHDF
                     EQFKLDCIKFFGIESVITAEDLKTRKIPELASKLYFEVQRAYDEKSKTVMENTYPIIK
                     QVYTNRGNEFKNIVIPFSDGQKSINIITSLEKSFQTEGKDLIKTFEQSIILAYIDQSW
                     KEHLRQMDDLKQSVQMAVHEQKDPLIIYKLEAFQLFKTMLSVVNKDVTSFLLKARVQI
                     EQPEEVKQAQQQRRVAPKTKEARGADELGHTNDAADTDTQQVEKQQPVVRTEQKIGRN
                     DLCPCGSGKKFKHCHGA"
     gene            24198..24608
                     /locus_tag="PLS16_03470"
     CDS             24198..24608
                     /locus_tag="PLS16_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60171.1"
                     /translation="MKNKIGFLILFTFSFIVLQAMDGEVELDTTQNNILFFKDPRIDI
                     LEKIYTYKKSNPFKNIRVHVFQATNRDAVFEMKNEFSKRFPGIPTFISYQAPNFKLRA
                     GDFETNQEASAFLKKVKPFFPSSFVIEETVKSNP"
     gene            complement(24739..>25031)
                     /locus_tag="PLS16_03475"
     CDS             complement(24739..>25031)
                     /locus_tag="PLS16_03475"
                     /inference="COORDINATES: protein motif:HMM:NF013038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HNJ60172.1"
                     /translation="TIGTLAIGYADGFDRRFGNGVGEVFIAGKRAKILGNICMDMCMV
                     DLTDIPEAKVGDEVEVYGKNISIIQQAEKIGTIAYELLTSISSRVKRVYFLD"
BASE COUNT         8674 a         3674 c         4186 g         8397 t
ORIGIN      
        1 ttttaaatag atttaagtct ttgccgtcca catcaaatct taattgttcg aaagcagtaa
       61 tctgtgcttt tttattatta gtaatattat taatgccgac atttaagact aaatattgtt
      121 cttttttcaa acctttaaac tgatttttca atctccaaga ataaccaccg aagatatcta
      181 aagtccaaac accttttgga ttaattcttt cttgacttac tatcgaattc cataaatcag
      241 atttataatc tactaaatca acggcttgag ctgttcttct tgatggacta atttctgtaa
      301 atgttctatc aacaaaattt actgttgaag taataaacca atattttgga gaacgataat
      361 tcaaacctaa actataagct tgttgtggag ttccgccgat ataataattt ttagtataaa
      421 ccgtgttgtt tggtagaatt acttcttgtg tattatctac ataagtagtg gtattccatc
      481 tgcttgaata ggtatatttt ccgattgaag cacctgcagt cattccaaaa cctttgtaga
      541 tactggcttc catgcctatt tctccaccgt aataagtttt gtcaatattc tgaatagaaa
      601 cgttcacaaa attataaacc aactcatcat aatatcttaa taaatctgta ccatcattga
      661 ttttagtata gaagccagtg gcttttaatc gaacttttgg agaatttaaa acatagccac
      721 cttcaacgct gtaaattttc tcattttgtg gtttgtctat ggtataatct ctagttctaa
      781 atgacacgaa aacattttct aacattggag cttgtgtttg tacaaatcca tttgcaaata
      841 aatagttttt gccattgatt ttataagtta aaccaccttt tacgccgtaa ttattaaatg
      901 aaagtgtttt agattttcct aatgaattat tcataaacaa accacttcta taattccctt
      961 ttctccaata agttgaattg gaatattcgg ctgcaacaaa aacatctaac ttattaaagt
     1021 ggtgtaccaa ttgtccccaa acaccagttt tgttgtaata taaattgaca ttgtattcat
     1081 atttatcacc aacttttatt tttctatcag gactgtctgc attgttttga ttttctacag
     1141 catcttttgg gaaattgaat tgtgcaaaac gattgatatc cacccaaaaa tcaccaccta
     1201 ataaatcttt catttgagca taattcttgc tcatcataaa ttgataattt aagccagcag
     1261 tcaattctgt attgtctttt atagtttggt tataaacagt attcaaatta acacgttgta
     1321 ttttttttac atcttgtttc aatgcataaa cagaacgtcc gcttccaaat tcaggatcaa
     1381 catggcttct attggattca tataatttat cccattgaac ttgtaataaa tcaggattgt
     1441 tgcgatacat ttctgctatc aaagcttgtt gaactggatc gctactgtaa gtcggtaaat
     1501 tcttgtaata ttgaggtctt gggtctggtg cattgtacca atctaaacca gaagtagaat
     1561 tttctccaaa tgaataggac aatgcggttg tcaaattctt gctgtcagat ggtttccatt
     1621 catgcatcaa gttgattaca ggttgatgtc tgtatccttc tctagcattt ctcttttttc
     1681 catcttgcca accccaattt ggattataat aatgactacc cgctaaatca taaaattgtt
     1741 gctcaacagg agtcgttttg ccatttttag taggtgcacc aaatgcggtt aaagaaattg
     1801 aattttttgc cattactttt tctatcgaaa gaaaataaga caaagattga taattcgtac
     1861 catcaacata gccttcttta gctaatcttc cagttaatga agctgcaaat gcccaacctt
     1921 tttttgttat tccagagcca taagtaatca tcattctatt ggtataggtt ctgtttgaaa
     1981 aagctgtagc aacggaaagt tgttttcttt gttttgaagc acgtgtatca atagcataag
     2041 taccacctaa agcaccaaaa gttaatgggt tgctttttat tccaatataa tttgttctat
     2101 ttcgagtaac atcgtttagt ccactccata aactatatgc accaaatcca ttatcaatat
     2161 aatcaattgg tgctccattc atataatttt cccaatattg actgtcatac cctctcattt
     2221 taaaacgtgc aaaactccaa ccaaaagttg caattgagaa aaatggatct cgagaagcac
     2281 ctaaaattga ggatacattt tggtcgccca tagatgttcc acctaattca gattcatcta
     2341 atgtaataac tgggatatcc atgtttgttc cagggtcaga aacggtagtt tttgtcttgt
     2401 tgctttcatc ttcagtatca acagttcctt cagattgagc atttcctgta aaagaaaaag
     2461 caagtacaaa agctattaaa aaaaatagat ttttgatatt catataaatt ggtattcttt
     2521 ttgagtatat aaagataaat gaattattca aatatcaaat acttaaattg gtatttttct
     2581 gattatttgc ttttaatcaa tagggaataa aaaattaaat tctatcttta gcttatgaaa
     2641 aaatatattt ttacattttc ttttttatgt tctattcttc ttggaaatgc acagaatagc
     2701 gaaaatcaat atcgtttaat agttgctggt ttttataatt tggagaattt atttgatacc
     2761 attgatgacc caattaagta tgatgaagaa tttacgccaa gtggtggtta tagatataat
     2821 acaacgattt ataaagataa attaggcaga ttgtcagatg tattatctca aatcggtaca
     2881 gatgtttcgc ctgatggatt agcatttttg ggaacagccg aaattgaaaa tgttgatgtg
     2941 ttgttggatt tggtagcaat gccaaaactt gcaagtagaa attacaaacc aatttggtac
     3001 gaaggtggag atgcacgtgg tattgattgt gcatttttat ataatcctaa atattttaaa
     3061 cctatctatt ctaaatcaat tccaattaat ttgaatcaag tagtaaaaga cggcaaacca
     3121 acccgagatg ttttgtgggt gcgtggtata ttaactggga ttgataccgt agatgttttt
     3181 gtgggacact ggccatcacg tcgaggagga gaaacattaa ctgctccaat gagaaattat
     3241 gtagcaggaa caattcgtca ttatgctgat tctatcagag caattaataa agaagcaaaa
     3301 atattggtaa tgggtgattt gaatgataat ccaacagatc ctagtttagt aaaatattta
     3361 gatgctgtcg gaacgaaaga taaaatgact acggaaaagt tttttaatcc ttttgtagaa
     3421 ttttacaaaa aaggatatgg caccttggca cacaatgatg cttggggttt atttgaccaa
     3481 attattcttt caaacggttg gatgaatacg aaacaagatg gttggtttta tcaaaatgca
     3541 catattttta agaaagactt tatggttcag aaaacaggaa aatataaagg ttacccaaaa
     3601 agaacatgga atttttctga atataattac ggttattctg accatttccc aacttatatt
     3661 cagttattac aaaaagtaaa atagtgtagc tattacttta gaacattagg caatctaggt
     3721 tcattaaaga atagattgtc ggtagtgatt gcattaatgc tgctttcata taatctaaat
     3781 tggaatgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaga ttgtcggtag
     3901 tgattgcatt aatgctgctt tcatataatc taaattggaa tgtatttaca ttaaaatctt
     3961 ttccttttgg atagaaagaa aattgaaagt cgataaaagg gaatcctaaa tctacacttc
     4021 ttaaacgaag tccaaaacca acaccatatt ggaagtaact ttgactaatt agtttttttt
     4081 cattatcgct aacccaacca acatcggcaa atgcatataa catacctttg ctcattgcaa
     4141 ttttcttatt tagaagaagg tttgtttcaa aacttgctgt tacacttttt gaaccacgta
     4201 aagtaggaga ataaaaccct cgaatcaaat tatttatatt aaaatatcta ttttcaggat
     4261 agaaaaatcc gtaattgaat tttgtactaa ttaactgtct taaaaaaaca tttttggaaa
     4321 ttttataact tggtgtgata taactttgag ctaagcttat tagcatttgc tcactttttt
     4381 tcttccttac aaaagtacta tatttaaagt cggtataaaa gtatcctact tttttaaaag
     4441 atttgccata atttgctttt agttccaaat tatttctcag acccacaatt tcatcaattt
     4501 ttggacctaa ccacaatgct aagctccatt ttttaggcag atattcagga atgtcgatgt
     4561 aaaagttatt tttaagaata acatattccc agtgtgcaaa tccaaatccg aataaaaaat
     4621 tggaattatt gatgaattgt tcattataat taccatctat aataaatggt ctatccatat
     4681 aattggtata gttgagtttc gtggataaaa taaatttaag tgtacttgct ttatttttct
     4741 ttttgatagg gaaagcataa gctaaccaca aattatattc atttgatttt gctccaacta
     4801 aggaacttgg gtctcttaaa ttattattta gactgacttt gatgttgtta aagttataat
     4861 taaaattaaa accccaattg gaatttaaag aaacaaaatt cttttttaac cctacagaaa
     4921 atttacgatt tagtttatca aaaacaaagt cagttttcaa atcaatctta gttccaaata
     4981 aattaaaata attataggca gtagagaatc ttaatggatt cagcttattg aatggcgttt
     5041 gaaacctcaa tgacaacata ttagggaagc caaataaatt attgatatac gttgcaactg
     5101 caaaactttc ctcaacatag ccagtagaaa tagtgtaaga taatttatct accaaaaaca
     5161 cataaacatc tacgccattt tcaatctcta tattttcatc atcacaactt tggctaaaac
     5221 taatatccac atatttatat cggttacgtt cccaaagcaa acgctctgta tcagcaaaaa
     5281 tacttgggtt tactttttct ccttttttaa agagtaaatc ttgacgagcc tgccattctt
     5341 tagaatttag atggagtttg ttgcccaatt tttggctttt tttaagttgt tcatattcat
     5401 ctgaaccaaa aggtggaagg atttttatat aaatattgac aatctctttg ttttgttcat
     5461 ttttatactt gtcaattcct ttatattgaa caccatctcc tcggtcgcta taattgatat
     5521 aggaaatata tttaaaggta ttttctactt ttgtttttct gtcttctttt ttatgaaatt
     5581 cctctaactc aaataagcta tcacgaatga ctatcaaact atcataaata gaaatgatag
     5641 aatctatttg ttgagcagta ctatttttat tgccaaaaaa tatagaaaag aataaaatga
     5701 actttatata cttctggtat tttgaataat ataaaaccat ttataacaaa actattagcc
     5761 tttaattttc tttagtgtag ctgagcctat ttttaataac attggtaaaa ctgaaacaaa
     5821 gacaatgcct ataataatct tttctaaatt atcttctacc cattttacat ttccaagtgc
     5881 ataaccagct attgtaattg ttcccaccca aataattgca ccaatgatat tatacaataa
     5941 aaatacattg tgcgacatat ttccaattcc tgcaacaaat ggcgtaattg ttctgataat
     6001 tggcataaaa cgtgcaataa caattgtttt actgccgtaa tgttcaaaat atttatggac
     6061 ttcatcaatc cattcttgtt ttaccatagg tttgcctcta aattttaatg ccaaaacttt
     6121 agtactaaaa tatttaccta cataataatt tacattgtcg cctaagatag ctgcaataat
     6181 caataaagga actaaaatcc aaatatttaa agctccagct ccaatagcac acaacattcc
     6241 agctgtaaac agtaaagaat ctcctggcaa aaaaggcatg attaccaaac cagtttcgac
     6301 aaaaataatt agaaaaagta ttccatatat aaagttggga tattgtgctg ttaggttttc
     6361 taatacttct ttggtgttac tcagtaactc taataaaaaa tgaatatatt ccattatgat
     6421 taataattaa aaatactttt aaaataggcg atagtagcag gcaatccttc ctcaaaagta
     6481 taatttggtt gatagtttaa taattcttga gctttagaaa tatctgcctg tgaatctcta
     6541 atatctccca ctcgagcttc tcgatgaatt gcttttatat ctttatgtag atatttttga
     6601 attgcttcat acatataatt gattgaaaag cgaccgccaa ctgctatatt ataggcatta
     6661 ttaaacgctt gattatttgt cgttgtcatt gctaaaatat ttgcctgaac agcattgtga
     6721 atatacgtaa aatctcgtgt ctgttcgcca tcaccattca caaagggagc ttcattattc
     6781 aataatgctt taataaataa aggcattaca gcagcatact cgccataagg atcttgtttg
     6841 ggtccaaaaa tattaaagta tctcaaacca attatctcca taccataagt ttttgaaaat
     6901 acatcagcat aaacctcatt agtatattta gttactgcat aaggagaaag aggcttacca
     6961 attcgctctt cttttttagg cagagttttt tcatcaccat aaactgacga agaagaagca
     7021 taaacaaaac gtttaatacc agcctctttg gcagcaaata acatatttac aaatcctccg
     7081 atatttacat tgttggtggt gattgggtct tttacagaac gcggtactga accaattgct
     7141 gcttgatgac aaatgatatc aattcctata caagcatttt tacaagtatc gaaatctcta
     7201 atatctcctt ctataaatct gaaattagga ttagaaaaaa aaggttcaat attttcttgt
     7261 ttgccagtgg ctaaattgtc taatactact acttttgcat gatgtaataa taaatattct
     7321 gcaatgtttg agccaataaa accagctcca cctgtaatta aaactttttt attggaaaca
     7381 tctgtattct ggaaagactt ttggtacatt cagttttttt tgtgaaaata caaaatttat
     7441 ctattaactt ctaatttccc atttgctaaa aatatacctt gcaaagcatc gtggtcaatg
     7501 gctattcgct ctaattctaa atctaaaata tttccttgta tccaattctt atcaacctga
     7561 aaactatcta attgttcttg tgcttgaatt tttgccaaaa acaattcttc tttgaaatta
     7621 taaactagaa attcattcaa aaactcaatg atttgtttgt ggtagtaatt atctacccat
     7681 tttataatca aacttttaga ttgtatagtg taattcagat ttcgagtttt aatggtgtaa
     7741 tttggattgt tgaacattac agatccagta aatatagcat ttccagtata ttctttcttt
     7801 aaaaaccatt tgtttgcata aataacaaat ggaacttcca cttgtaattt agtcaataaa
     7861 ctacttactt taaattcttt aagcaaaata gcatactcat tttcttctat atcatacttt
     7921 tggttggcaa aatgatgatt taaaaaatta ccgacacttt gataatctgt ttgggctttg
     7981 ataataacct tgaacttata cgaagattga atgtcattcc aatttaaaaa atctaaagta
     8041 agataatttt ttactcgatt gattcttctt tgtacaatat tttcgatata acctttgata
     8101 tctaaatcta cttttgcacc atctatctca atatcagttt ttgtggtata taattcaata
     8161 ggttttaagg ttaataattg ataccatttc ttttcgttaa ataaatgttc acgaagctga
     8221 taccaaatat ctttaaagat agaatcttgt atgatacgct caaaaatatt cacacttcaa
     8281 aaatacagta tcgaattgaa attttaagct ttaatatctg atattataaa taatataata
     8341 ataagacagt tgcaaaagtc aaattacaat aatattctag gctttcattt atttttacaa
     8401 tgcagttatc gtattgataa tgaggattaa aaataaacgt ataacgaaga agattgaagg
     8461 aattcggctt gaaatttatg aaataggatt gccttgcaaa gtttttaaaa tgcttttttc
     8521 taattgttgg tatgaaatac gcattctttt ggaaaattgg tattctttag catattttgt
     8581 atttttgttt tatgattcaa gaacaagaac tccatcaatt tatagaacag gcattatatg
     8641 aagatattcg aaatatagga gatttgtctt ctttgtcttc aattgataga gatacaaaag
     8701 gcactgcaaa acttttggtt aaagacgaag gtattctttg tggcatgtct gttgcaaaag
     8761 ccattgctca aaaggtagat gacactttgc aatttgaaga attaatcaaa gaaggaagta
     8821 ctgtaaaatt tggtaacatc gcattttatt tggaaggcaa tgcaatttct attcttactg
     8881 ccgaacgctt aattttaaac tgcatgcaac gtatgagtgg cattgctaca acgacacaga
     8941 aatatgcaaa agccattgag ggaacaaatg ctaaagtaat tgatacccga aaaacaacgc
     9001 ctaatttaag attcttagaa aaatatgccg ttaccgttgg cggaggattt aatcatcgtt
     9061 ttggtttgta tgatatgatt atgttgaaag acaatcatat cgacttttgt ggaggaataa
     9121 caaaagctat tcagaaagtt agaaagtacc tatcagacaa taatttgtcg ctaaaaatag
     9181 aagtagagac aagaaatatt gatgatgtaa aagagatttt agcttgtgga ggtattgata
     9241 gaattatgtt ggataattac aagccagaag attgcaaaat ggctgttgag ttgattaata
     9301 agcaatatga agttgaagca agtggcggaa tcacactaga aaccataaga gcgtatgcag
     9361 aaacaggcgt agattttatt tctgttggag cattaacaca ttctgtaaaa agtttagatt
     9421 taagcttgaa agctactttt aaataatatt tttatatttg ccctatgtca aaaagagtta
     9481 tttatacaga aaatgcacct gaaccaattg gtccttattc tcaagctatt ttagtggata
     9541 atacattata tgtgtctggt cagattgcta ttgatccatt tattggagat gttgttagaa
     9601 acggtgttga gggcgaagcc attcaagtta tgaaaaattt agaaagtatt ttaagagctg
     9661 ccaatatgga tttttctaat gttgtaaaat gttcaatctt cttgaaagat atgcaagatt
     9721 ttcctaaagt aaatgcggtt tatgggaaat atttttcaga gaatcctcca gcaagagaaa
     9781 ccgtagaagt cagttgtttg cccaaaaatg tattggtgga aatttcttgt attgctgttt
     9841 attaagcaaa ttttcgactt aacatttgca aatttttaat gtacgaataa atgtactacg
     9901 ccattttcta ctaaaaaatg cttattttgt aatatcaata gataaaaatg tcattaacca
     9961 atcatatacc atttgatgaa attgtgcaga aatctaaatc ttctgctcct aaaatatcgg
    10021 cacatcaaaa atatgttgca ggaattgcac cttttttgcg aggaccatat tcttctatgt
    10081 atgtctctca accttggaca atacgtcaat atgcaggatt ttcgaccgca gaagcatcca
    10141 atcaatttta taaaagaaat ttggctgcag gacagaaagg cttgtcagtt gcatttgatt
    10201 tagctacgca cagaggttat gattctgacc acgaacgagt tgtaggcgat gttggaaagg
    10261 caggcgttgc catagatagt gtggaggata tgaagatttt atttaatgaa attcctttag
    10321 atcaaatatc agtatctatg acgatgaatg gtgctgtttt gcccatttta gcattttaca
    10381 ttgttgctgc cgaagaacaa ggtgttgccc aaagtttatt aagtggtacg atacaaaatg
    10441 atattctaaa agaatttatg gtgcgaaata catatatata tccaccgcaa cattctatga
    10501 aaatcatatc agatattttt gaatatacga gcaaaaatat gccaaaattt aattcaattt
    10561 ctatatcagg atatcacatg caagaagctg gagcaactcc agaaattgaa ttggcttata
    10621 cattgtctaa tggacttgaa tatatcaaaa caggattagc agctggttta aatattgatg
    10681 attttgcact aagattgtca tttttctggg caattggtat gcaacatttt gaagaaattg
    10741 ctaaaatgag agctgctcgt ttattgtggt caaaaatagt agctgaattc catcctagaa
    10801 aagaaaaatc gatggcattg cgtacccatt gtcaaacttc aggttggagt ttaacggagc
    10861 aagatccata caataatatt acaagaacct gtattgaagc aatgtctgca gtgtttggtg
    10921 gcacacaatc attacatact aattcttttg atgaagcgat tgctttacca actgattttt
    10981 cagctaaaat agccagaaat actcaaattt atttacagaa agaaacaaag atttgtaata
    11041 ctgttgatcc ttgggctggt agcgaatatg tagaacaatt aacgctatct attgctgaaa
    11101 gagcttgggc tttaatgcaa gaagtagaga gttatggagg aatgacaaaa gcaatcgaag
    11161 caggcatccc aaaaatgaga atagaagagg ctgcagctat taaacaagcc aatattgata
    11221 cagaacaaga atatattatt ggtgttaatg tgtttcaaac ggatgaagaa acaccatttg
    11281 aaattttaga gattgataat actgaagtac gtgcaaaaca aattgaacga ttattacaaa
    11341 ttaaaacaac aagagataat aatgcagtac gagttgcatt gcaaaatata gaagctgcga
    11401 caaaaagtgg caaagaaaat ttattggctt tatgtattga tgctgctaga aaaagagcta
    11461 ccttaggaga aatttcctct gcaatggaaa aagaatttgg gcgatataaa gcaaatatca
    11521 aaacaattag tggcgtgtat caaaaacaat caaaatcaaa tcctattttt gaagaagccc
    11581 aaaaagcaac ggatacattt gctgaacaaa atggacgaag acctagaatt ctaattgcta
    11641 aaattggaca agatggacac gatcgtggag caaaagtgat tgcaacagca tttgcagata
    11701 tgggttttga tgtagattta tcgcctttgt ttcaaacacc agaagaggta gcacagcaag
    11761 cagcagataa tgatgtacat attgtaggag tttcttcttt ggcggcaggt cataaaactt
    11821 taattccaca actgattcaa gcattaaaaa aaataggcag acctgatata aaagttgcct
    11881 gtggaggtgt aattcctaaa caagattatg attatttata caatgcaggt gtaattggtg
    11941 tatttggtcc aggaactgta attgcgtctt gtgctttaga tgttttaaag aaaatataaa
    12001 tcaataattt gttttaatct aaatggctta ttcaagaata tttgatatta tccaacatca
    12061 actccaaacg aatcctttag aggtttgctt ggctagaaga tacgataaaa agaattggac
    12121 gacttattct actgaaaaag ttattgaaat tgccaataaa attagttctg gacttttaca
    12181 attaggttta aaaaaaggcg ataaaatcgc gatagtttcg ataaataata gaccagagtg
    12241 gcaattttta gatttagctt gttctcaaat tggagtaatt acagtaccta tttatccaac
    12301 tatttcgagt gcagattatg aatatatttt caaccatgct gaagtaaaat atctttttgt
    12361 ttctgataaa attatacatc gaaaattgag agaagtagcg gctaaaatta caacaattca
    12421 agcggtttat agttttgaag aagtagattc aattgcttca ctacaaagtt tatatggaaa
    12481 tattaatcaa aaggcaattg atgaagttaa agcttcaata gatgttcatg atgttgcaac
    12541 aataatttat acttctggaa ctacagggat tcctaaaggt gttatgctaa cacataataa
    12601 tattgtatct aatgtcaaag atgcgttatc gataattacg gtaacgaaaa atgcaaaaac
    12661 tttaagtttt cttcctctgt gtcatgtttt tgaacgcacg attaattatg cttattttgc
    12721 agctggtgct tcaatttatt atgctgatgg attagaaact atttcagaaa atatacaaga
    12781 cattcgacct gattatttta caactgtacc tagattgtta gaaaaagtat ttgagaagat
    12841 tgttaaaaag ggaaaaacat taacaggagt tcagtataaa ttgttctttt gggctttaaa
    12901 attagcagaa aatacacctt tagaaaagga aaaatcattt atacaaaaaa tcaaattttc
    12961 agttgctgat aaattaatat tttccaaatg gagagccgct ttaggtaata atataaaagc
    13021 cattatttgt ggttcagcac ctttacaacc tcgattggca acgattttta ctaatgcagg
    13081 cattcctgtt ttggaaggtt atggactgac agagtgttcg ccagtcgtga gtgtaattcc
    13141 tttagataaa aaatgtttta gagcaggttg tgtaggaaaa gtattgccct ctatcagtgt
    13201 tcgtttagca gacgatggag aaatcttggt aaaaggtcca agcgtaatga aaggctatta
    13261 caaagatgat gacgcaacaa aagcgacaat cagcgatgat ggttggttgg ctactagtga
    13321 tattggtgag tttacaacag aaaaattttt aaagattaca gatcgaaaaa aagatttatt
    13381 taaaacctct ggtggaaaat atattgcacc tacaccaata gagaataaga tgaaagaatc
    13441 tttctttata gaaaatattg ccttagttgg tgatggagaa aaatttgtag cggctattat
    13501 tcaaccgaac ttctcggaat tagcaaattg ggcaaaagat aagaatatca atttccaaac
    13561 gaatcatgat ttgacggaaa atttagagat aaagcaacta tttaaagata ttattagtaa
    13621 ttttaacact tgcttgggaa aagtagaaca agtaaaagat tttattttgg tagatgatga
    13681 atggagtgtg gacaatgaat tacttacacc aacaatgaag ttacgaagaa aaaaaattaa
    13741 agaattctat caaaaagaaa taaattcaat atacaataaa taaatagaca tggaagtaaa
    13801 aagattattt gataccattt attatcaaaa gagcaaacac ccacaacaag tggctttggc
    13861 ttctttacaa aatggagaat ggagaacatt aagcaccgac gaccttatta atgaagccaa
    13921 taaattaagc ttaggcttat tgcaattagg cattcaaaaa ggagataagg ttgctgttgt
    13981 aacttcagaa agtagagtag aatggaatat ctgtgatatt gcaataagtc aaattggagc
    14041 aatctcagtg cctttatatc caactatttc ttctaaggat tttgtgcata ttctaacaca
    14101 ttcagaagca aaaatttgta ttgcatctga taaaggatta tttgataaaa tcaatagcgt
    14161 aaggtcagag attccaggct tacaagagat ttatactttt gaccaagttg aaggagctaa
    14221 aaattatgct gaaattagca acttaaatcc taatggtgat attgcacaaa ttaaagctat
    14281 ttcagatact attgaagaag atgaattggc cactttaatt tatacttcag gaacaacggg
    14341 tttgccaaaa ggtgtaatgc tttcacataa aaatgtggtt tcaaatgttg taacagtatt
    14401 ggcggagtta cctattgaag caggacatgt ttctttaagt ttcttaccat tatgtcatat
    14461 ttttgaaaga atggttattt atacttacct aatgggtggt gtaaatgttt attattctgg
    14521 tatagacact ctcgctgaaa aattaggcac agtaagacca catttcttca caacagttcc
    14581 aagattattg gaaaaagtgt atgagaaaat tgtcaataaa ggattagcat taacaggctt
    14641 aaaaagaaaa ttattcttct gggctttgga aatggctgac caatacgagt atgatcaaaa
    14701 accaagtttt actttcaaaa ttgcagataa attaattttc agcaaatggc gtgaagcatt
    14761 aggtggtcga gtaatgggta ttgttactgg ttctgctgct tgtcctttga aaattgctag
    14821 agttttctct gctgcaggtg ttccaattag agaaggttat ggtttgacgg aaacttctcc
    14881 agtaattacc tttaatagat tttcagaagg tggtgcgatg ttaggcactg tgggtatggc
    14941 aattaaagat gtggaagtaa aaattgctga agatggagaa atcttagcaa aaggccctaa
    15001 tattatgcaa ggctattaca aagaacctga taaaacggca gaagtattta cggctgacgg
    15061 ctggtttatg actggtgatg ttggaacatt tgttactaat aattatggta ataaattttt
    15121 aaaaatcaca gatagaaaaa aagaattatt aaaaacatct ggtggaaaat atgttgcacc
    15181 agctccaata gaaagtaagt ttagagagga tttctttgtt gagcaaatta tggtagtggg
    15241 agaaggcaag aaatttgttt ctgcattaat tgttccagca tttccagtgt tagaagagtg
    15301 ggcaaaagaa aataatattg cttttacaga cagacaatct ttaatttcaa attcaaaagt
    15361 attggctaaa atccaaagtg ttgttgatga attaaatcct aattttagcc atattgagca
    15421 aattaagaaa ttcaaattat tggcaaatga atggacaaca gaaacgaccg aattaacacc
    15481 aacaatgaag ttgaaacgta aagtaatctc agcaaaatat gctaaagaga tagatgaaat
    15541 atacaacaac taatttagtt tagtcaaaca ttgatagttg tttattgatg ttaaaagtaa
    15601 gtctgtgtag gggcgaatat ccaaaactcc acacagactt tttatgttgt agtgttggat
    15661 agcccttgtt ttgttgccaa ttgtattgtg gaaatttttt tgaatacgct tccatcaaat
    15721 catctctgtg agtctttgct aaaatagatg ctgctgcaat ttcaatatat gtagcatcac
    15781 ctttgatgat agtttggtgt gctatttcag gataaggttt gaaacggttg ccatcaataa
    15841 ttaataaatc aggaataggt tgaatttgag caattgcttg atgcatagcc aaatatgtgg
    15901 cttgtaaaat atttactttg tcaataattt gattgtcgat aatcccaaca gaataagctt
    15961 ttgcttcttt tttgatgagt tctgctaatg cttttcttgt agcttcattc agttttttag
    16021 aatcattaat cagaggatgc tgaaaacctt taggtaaaat aacggctgcc gcaactacag
    16081 gtccagcgat acaacctctt cctgcttcat caacaccaca tactatactt tcacttggat
    16141 aatgaatgag catatcaaaa attggaaaaa agtttaatta ttaatatcaa aacttatact
    16201 gctgaaaggt aacattattt gtgtattttt cccttcattg ttgagttttt tcaatacttt
    16261 atctaaatgt gtatctcgca ttacttctat atttttatca taaggtttga agaagttgtt
    16321 ccaatgatgt aatatgattt ttttaggctt tcctgctgca taaactttgt ttaatcctgg
    16381 caaacaggct ttttctttcc aaaatatttg taaagcgata tctacatttt tgttttcaca
    16441 aagtgctcta actttttcta ctgatgcatc tgtaatattt ccacttaaaa ataaacatcg
    16501 ataaacgata gaatcttttt ccaatatatc tatcaaataa gaataattgc cgtgtaattt
    16561 ccatttccaa aatcgatcag gtaagtgttt aagtggctct tgatacaaac ctgtaaataa
    16621 agtaatgtct gcaaaatgtg gactgtgtgg gctatttaat ggaaatattc tgaaagtact
    16681 atcggaacta taatgatagc tttgcatgtc gttttcaaat tgtacatcaa agttattttt
    16741 aactgaatta atttcattag taatatgctg ttcggcaatt atttttgttg gcacaccttt
    16801 cgttaaaaag ttctcgatat tcaaacaatg gtcataatga ccatgtgaaa taacaatggt
    16861 atttgcattt tcataaattg accttggaag tatattttca gacaattttg ggaattgtaa
    16921 tttttctaca aaatttttaa atacatcggg attactaaaa tatggatcag taatcatttt
    16981 tttaccttta taatttataa caaaacccga agtacctaaa aaagttagtt gtacatcttt
    17041 tgtagtatca taatttgatt ttgcaacata attgccaaaa tcataatcgc taaatggctt
    17101 acctattgtt tttattttta aagttgccaa taaactaaca attgttacaa aagctacaat
    17161 tatcttaaag agtcgtttca tatttttatt ttgatagact aatatacata atattaattt
    17221 cagaatgtcc tattttctgt ctattttagc cttattcaaa cttttaccaa ttatggaaac
    17281 aatattacct ttattagaac aagttagtgc agcaagtgta gatgcaaagc gattaacaga
    17341 cgtacaaaaa gttgcctttt tagacacatt ggcaacttta ttagttcaaa atacagattt
    17401 gatagttgca gaaaataaaa aggatttaga taaaatggaa gatagcaatc caaataaaga
    17461 ccgattgtta ttgaatgcaa ctagaataca agacttggca aatagtttac acgatgtcgc
    17521 aaaattagaa gacccaactg gtaaattatt atacaaaaag acgattgata acggattgga
    17581 gattgagaaa aaaacagtgc caattggcgt tgttggtgtc atttatgaag caaggcctaa
    17641 tgttaccatt gatgtcgcag ctttgtgtat tcgctctggc aatgctgtgg ttttacgtgg
    17701 aggaagtgat gccgacttta ccaataaaat tttagtacaa ttcattcatc aagcactaga
    17761 gaaaaatcaa cttaataaag agattgtaca gttgatgcct gttgatagaa agtttgttaa
    17821 ggatttattg gaggcggttc aatttgtaga tattatcatt cctcgtggtt cacaacaatt
    17881 gatagatttt gtacgtgcta attctaaagt gccaacgatt gaaaccggag ctggtgtttg
    17941 tcatacttat gtggaaagta ctgccaattt agataaggct gcaaaaattg tggtgaatgc
    18001 caaaactcaa agaccttcgg tttgtaattc cttagatact attttaatag ataaaacagt
    18061 tgcaaaagat tttctgccta aaattattga tggatttaat caatttggcg tagagatttt
    18121 tgcggatgaa gatgcttatg caattttaaa taaagcaaat tattcaggat tacataaagc
    18181 aactgaagag aattttggta tggaatattt gagtcagaaa tgttcagtta aaacagtaga
    18241 aaatttagag gaagcattga cgcatattcg taaatattca tcaaaacatt cagaagcaat
    18301 aattagcgaa gatgctgata agatagctag atttctaaat gatgttgatg ctgctgcggt
    18361 ttattcaaat gcttctactc gttttacaga tggtggcgtt tttgggttag gtgctgaaat
    18421 tgggatttct actcaaaaat tacatgcaag aggacctttt gctttggaga aattaactac
    18481 tgaaaaatgg tttgttaaag gaaacggaca aactagataa atactaatat aataaataga
    18541 aaatgaaatt tcaattacaa attttagtgt ttatatgttc cgcttttgca ataagttgtg
    18601 gttcttcaaa aaaaaccact gaaacaagag gacaattata taaatcggaa gatttagtga
    18661 ttaatcaaat ttccccaaat gcttttgttc atacctcttt cttacaaaca aatgattttg
    18721 gcttagttcc atgtaatgga cttattgtta gagataaaaa tgaagtcgta atttttgata
    18781 cgcctgaaaa tgacaaaagt gctgaagaat taataaaatg gatttcaaat aaattaaact
    18841 gtaaaataat tgctgtaatt ccaacacact ttcatgcgga ttgtttaggt ggattgaata
    18901 catttactaa atacaaaatt ccttcttatg cttatttcaa gactattgag tttgctaaag
    18961 aaaataaagt tgcagttcct gaaataagtt ttaaagattc tttgatttta aaagttggca
    19021 ataaagaagt tattgcaaaa ttttttggag aaggacatac aaaagacaat gttgtgggct
    19081 atttccctag tgaacagatt atgtttggag gttgcttaat taaggaatta aatgccacaa
    19141 aaggttattt gggagatgct aatactaaag attggtcatc aactgtttta aaagtgaaat
    19201 ctgcttatcc aaatgtaaaa attattgtac caggacatgg aaattatggc gaccagaaat
    19261 tactcgacta tacaattcag ctttttaaat agcttgtgtt tcatcaaaat tttctaaaat
    19321 agtagcgaca cgttttacaa attttccacc caacatacca tcaaccactc gatgatcata
    19381 agtgtgcgat aaatacatca tacttcttat agcaataata tcttcatctt ctaattcaac
    19441 aacgacaggt tttttggtaa ccacaccaac tgccataatt gcaacttgtg gctgtggaat
    19501 aattggagtg cctattatat ttccaaaggt gccaatattt gatacagaat atgttccgcc
    19561 tgaaatatca tcgatagtta atgaattggt tcttgcttta tttgctaata aattaatttc
    19621 ttgggcaatg cccaaaatat ttttttggtc tgtgcttttg atgacgggta ctattaaatt
    19681 gccattgggt aatgctgttg caattcctat atttatttgt tttttataaa taatattatc
    19741 accatctact gaaatattta tatctggaaa ttctttgatg gcttgagcaa tcgcttcaat
    19801 aaaaacgggc aaataggtaa gattgaagcc atatttttgt ttaaaaattt ctttattttt
    19861 tgctctccat ctaacaattt tgcttacatc aacctcaaca aatgaagaaa catgagctga
    19921 aatttgttta gaggctacca tgttttttgc aattagtttt cgcatcctat ccatttctac
    19981 aatttcatcg ccaagtacaa ttttaggagc aattttagtt gtaacttcat ttactttttc
    20041 tatacttgaa gtagaaatag tttggatgtt tccttttttc ttctgttcag cataagctaa
    20101 aatatcttct ttggtaactc tgccacctaa tccactacca ataatactat ctaattcagt
    20161 cgcacttatt ccttcttgtt tggaaatatt taaaaccaat ggagagtagt tgtttgaata
    20221 ttggattgct tcttttggtt taggaagctg tatgtttttc tgctcgatag atataacttc
    20281 ttgagtaggt attgttgctt ttatttcatt tttttcttca acaataggta cttcttcttt
    20341 tgcttggttt tctatcgttt tttgaacttg ttcagtctgt tcagtttctg gatttggtgt
    20401 tgatacactc acatttgtat ctgtttcaat aatagcaagt gcctcgccaa tgggaataat
    20461 atcattaggc ttaaaaagga ttctttttat agttccactc actggcgaag gaacatcaga
    20521 atctacttta tcagtagcaa cttctaataa tatttcattt tcagcaattg tatccccttc
    20581 ttttttatgc caacttaaaa tagttgcctc gataatactt tcacccattt taggcataac
    20641 taccttatat tctgccatat ttttttgctt tttaatatta aattaataca atatcaacgc
    20701 ctcaccaatt attttatgaa ttaaagttaa ataatatttt ctaagttcaa taatgtttct
    20761 ttttctcatt ttaaattggg gataaaatcg taaatttgct atctcaaaat tcaaaaaata
    20821 gtacatgtta aaatttataa ccaaaggttt atctaaagta ttcggaagta aatcatcaag
    20881 agatataaaa tctataatgc caattgttga acaaatcaat atagaatttg ctaaactttc
    20941 aacaatttct aatgatgagt tgcgtggtaa gacttatgaa tttaaaaata gaattgtaca
    21001 ttatttatct gcttttgatg agaaaatcaa cgaactaaaa gagcaagcgg aaaaagatga
    21061 tgtaaatatt gaagagaagg aaactatttt taatgaaatt gataaactaa ccaaagatag
    21121 agacgcagca attgaagaaa tattattaga aattctgcct gaagcttttg ctgtggttaa
    21181 agaaacagca agaagattta gtgaaaatac tactttagaa tctacagcca cacaattaga
    21241 taaagatttg tctgtcaaac atccgcacat tacaattcaa ggcgataaag taatttatca
    21301 aaatagatgg ttggcagctg gaaatgaggt ggtttggaat atggttcatt atgatgttca
    21361 gttgataggt ggtatcgttt tacatcaagg aaaaatttca gagatggcaa caggtgaggg
    21421 taaaactttg gtttcaactt tgccagctta tttgaatgca ttagcagatt gtggggtaca
    21481 tattgttacc gtgaacgact atttagcaaa aagagatagt gagtggaatg ctccaatttt
    21541 taatttcctt ggtttgacgg tagattgtat agacaaacac gagtcacatt ccgaaggcag
    21601 acaaaaagct tatcgtgcag atatcactta tggtaccaat aacgaatttg gttttgatta
    21661 tttgcgagat aatatgtcaa gaaccaaaga agaacaagtt cagcataaat tgcattatgc
    21721 aatggtcgat gaggtggata gcgttttggt agatgatgca agaacaccat tgattatttc
    21781 aggacaagtg ccaagaggcg aggaacaaat gttttacgaa ctaaagccaa gaattgaacg
    21841 aattgtcaat gctcaaaaga aattggtcgg ggattattta tcagacgcaa aaaaattgat
    21901 agcaaataat gtaactggtg ttaaagatgg cgaaggcgga ttagcattat tacgagctca
    21961 tagaggtatg ccaaaaaata cggctttgat taaatattta agcgaagttg gcgttaaaca
    22021 aattttatta aaaacagaaa atcattactt gcaagaacaa caaaaacaca tgccttttgt
    22081 cgatactggt ttgttgtttg tgattgatga aaaaaataat cagatagaat tgactgaaaa
    22141 aggcttggaa atgatgacaa cttctggcga agaatctgat ttgtttatta tgcctgatat
    22201 tggctctgta attgcagata ttgaaaatga agaaacactt tcagcatctg aaaaaattca
    22261 gaaaaaagac gaagctattc aagattatac ccaaaaagca gaaagagttc atacgattaa
    22321 tcaattatta aaagcatatt gtttatttga acgtgatgta gaatatgtgg tgatggaaga
    22381 taaagttaag attgttgatg aacaaactgg tcgtatcatg gaaggtcgtc gttattctga
    22441 cggtttacac caagccattg aagctaaaga aaatgtgaaa gtagaagccg catcacaaac
    22501 attcgcaacc attactttgc aaaatttctt ccgaatgtat cacaaattag caggtatgac
    22561 cggtactgct gaaacagaag ctggagagtt ttgggaaatt tataaattag acactgttgt
    22621 tattccaacg aatagaacaa ttgcaagaga tgacagagaa gatttaatct ataaaaccaa
    22681 aaaagaaaag tacaatgcga ttatagatga aatttcaaaa ttaaaagctc aaggcagacc
    22741 tgttttatta ggaacgacaa atgtggaggt ttccgaattg ttgagcaagt tattgacaat
    22801 gagaaaaatt gaacacaatg tcttgaatgc gaaacaacat caaagagaag ccgatattgt
    22861 tgctgaagca ggacgtgctg gacagattac aattgcaacc aatatggctg gtcgtggtac
    22921 cgatattaaa ttaacagatg aagtgaaagc ggctggtgga ttagcaatta ttggctctga
    22981 aagacacgat tcaagacgtg tggatagaca gttgcgtggt cgtgctggtc gtcaaggtga
    23041 tccaggttct tctcaatttt atatttcatt agaagatgat ttgatgagaa tttttggctc
    23101 agaaaaatta tctggtatga tggataaaat gggttttaaa gaaggtgatg tcattcagca
    23161 ctcttgggtt tctggttctg tggaaagagc tcaaaaaaaa gtagaagaaa ataatttcgg
    23221 aattagaaag cgtttgttgg aatatgatga tgtgatgaat gctcaacgtg aagtaattta
    23281 taaaagaaga aatcatgctt tggctgggga acgtatttca ttcgatttaa tgaatatgtt
    23341 ctttgatttg gcagaagaaa ctgttgagaa tgctaaacaa aatcatgatt ttgagcaatt
    23401 caaattagat tgcatcaagt tttttggaat agaatcggtt attactgctg aggatttaaa
    23461 aactagaaaa attcctgaat tagctagtaa attatatttt gaagttcaaa gggcttatga
    23521 tgagaaatct aaaacggtaa tggaaaatac atatcctatt atcaaacaag tttatacaaa
    23581 tagaggaaat gaatttaaaa atattgttat accattttcc gacggacaaa aaagtataaa
    23641 tatcattaca agtttagaga aatcatttca aacggaagga aaagatttga ttaaaacttt
    23701 tgaacaaagt attattttag cctatatcga tcaatcttgg aaagaacatt tacgtcaaat
    23761 ggatgatttg aaacaatcag ttcaaatggc agttcacgaa caaaaagacc cgttaattat
    23821 ttataagtta gaggcattcc aattgtttaa aacaatgttg agcgtagtca ataaagatgt
    23881 aacttctttc ttacttaaag caagagttca aattgaacag ccagaggaag tgaaacaagc
    23941 acaacaacaa agaagagtgg ctcccaaaac aaaagaagcg agaggtgcag acgaactagg
    24001 acataccaat gatgctgcag atacagatac gcagcaagtg gaaaaacaac aacctgttgt
    24061 gcgtacagaa caaaaaatcg gaagaaatga tttgtgtcca tgtggaagtg gtaaaaaatt
    24121 taaacattgt cacggagctt aatttggaat gagatttgct tttaacaata aataggttta
    24181 gatgtaattt tttataaatg aagaataaaa ttgggttttt aatattattt acttttagtt
    24241 tcattgttct acaagcaatg gatggagaag ttgaattgga tacaacacaa aataatattc
    24301 tcttttttaa agacccaaga attgatattt tagagaaaat ttatacgtat aaaaaatcca
    24361 atccatttaa gaatattcgt gtccatgttt ttcaggccac aaatagagat gctgtttttg
    24421 aaatgaaaaa tgaattttca aaaagatttc caggcattcc aacttttatc agttatcaag
    24481 ctccaaattt caaattgaga gctggagatt ttgaaaccaa tcaagaagca tcagcattcc
    24541 ttaaaaaagt aaaaccattt ttcccttcaa gctttgtaat tgaagagaca gtaaaatcaa
    24601 atccctaaat ggtttcgttt ttaattaatt tgtctctttt ttaatcttct caaaaaattg
    24661 ttaatactac cgttgtgaat ttttggacta atgatacact taattggtct aatgaatcaa
    24721 agaggtattc tcaatcattc aatccaaaaa gtaaactctt tttactcttg atgaaataga
    24781 agtcaatagt tcataagcaa ttgtaccaat tttttcagct tgttgaatga ttgaaatatt
    24841 tttaccataa acttccactt catcaccaac ttttgcttca ggaatgtctg ttaaatctac
    24901 catacacata tccatacaaa tgttacccaa tatttttgct cgtttgcctg caataaaaac
    24961 ttcgcccacg ccattcccaa atcttctatc aaagccatct gcatagccaa ttgctaatgt
    25021 accaatagta g
//
LOCUS       DAOQCW010000023        24508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_764_length_24508_cov_5.796385, whole genome shotgun sequence.
ACCESSION   DAOQCW010000023 DAOQCW010000000
VERSION     DAOQCW010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 24508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24508
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_764_length_24508_cov_5.796385"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PLS16_03480"
     CDS             complement(<1..180)
                     /locus_tag="PLS16_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60173.1"
                     /translation="MQHSLNFDNLDGKLAQDEAEHRHAESKLTIEDNIEQEPNVKIVE
                     STAGNKGGTSTKKLAD"
     gene            549..1619
                     /locus_tag="PLS16_03485"
     CDS             549..1619
                     /locus_tag="PLS16_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60174.1"
                     /translation="MKLKLALLSLCILFVLFANANMASPIRPGTKTSTVFSSKDIDIL
                     SESIHIKIAPDFKTAKFTVVYNIQSNSSGKQIPLLFYAQAYNDKFIVLLDDHQVPIQD
                     IPADFIPSTSINGFKNNFDKVEITWNKDYQPTYMLQDLKYFETDISKGNHRIKVSYTA
                     NVWADVSGWTKKYSFYYSLTPAKFWKSFGTLDISLEQAGNIKQLTTNIGLSEEKKFQQ
                     KNNWHFNQLPDEYLEFSYTPNPNKLASILILIEPSGIALLVGLILLVLHLILIVLYRK
                     NYPNKKHSIVVSLGSILVPLLFLLSYIYSFIIIDDIIGADAGKSHGYSAILALMLYPV
                     LLPIYGLFMWLIDKIIKRKISK"
     gene            complement(1625..4378)
                     /locus_tag="PLS16_03490"
     CDS             complement(1625..4378)
                     /locus_tag="PLS16_03490"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNJ60175.1"
                     /translation="MKFIEQIVEHIKHTESCENLQNQLFLFPSKRAVALFNDLLQNYF
                     ENEIFVFPKVQTIQSFFIENSNYNVVDEYTLLQELYFVNEAVTQTPQPFHQFLGWGKL
                     VLKDFDEIDKYLVDTKYFFDVLLAHKEVEAHFNVDEIVKEYLESFFTNIHNEKQGAFF
                     EDFIQTWKYLGEIYNHFGQRLLDKNIIYEGKAFRLLLKEIESNQKQLPYLKIHFCGFN
                     AFTTCEKKLLDTLISQYSVNTWWDIDEAFVKNEYHEAGNFLRKYYQEYNGEQHHWISD
                     KTLNKSIEVVAMPTNVGQVHYVRSNMNIDENNCIVLCDENLMDYCLPIFNAEATNITM
                     GLSINKMSVFELLSTYISLLNSMVLSDENTYIKGETALPFFISGLLEKQIPKRGELFK
                     HLYNSQIISLSLVQTLISDDLYQLMAKPFNVSTIFQNLLNLIQLSNAEQYLKQTLMEH
                     VSTIQFTTQQHIIQSAEDLLVILNMYVGNQSIAYETKLDSKIQIMGFLETRLQDFDNI
                     YILSLNDSIMPGTNRTNSFIPYNLRKSFHLPTFDQFDGINAYHFYRLLKRGKNITLIY
                     NNSSIDNEEEKSRFIRQIESEFETETIIQQSVLMKNNDDKKINKAIEFNKTKAIIDKL
                     KERIYSPSSLKLYLSCPVQFYLKYVVGISEPKELYANFDSAYFGTILHKILELYYSDF
                     QNSVELKDIDTYFNQAISDPKIVPLNYFTGKRVLDKKVIKKLVLDILKNDSEEDFKIV
                     GLEQNYSTEVYLHDFKIKINGNLDRVDRIDNSIRILDYKTGEVKLLAFPDENKKSKTK
                     NNKPEESEIDKFFNKLFGLDNGGFPETFQGLMYAWLYSKSIDVHAKDVSISVGFYLAK
                     KLQGGIQYLNDGNPIPKAILDEFEQRLFALLQEILDIKTPFVINAESKSYTYSAYADL
                     LSL"
     gene            complement(4524..4952)
                     /locus_tag="PLS16_03495"
     CDS             complement(4524..4952)
                     /locus_tag="PLS16_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60176.1"
                     /translation="MKKVLLYALILISMVSIVPSCTKEDTKLSAKEEVVKLLTTGRWY
                     YQERYRNGVLEADCFNKSHFFLFSPDGTMIASYTSLQSGVYSVDENGIGMSITLDGAP
                     KIVFTSVMFSESNSKLSIEGTFSSDKVKIVLSKTPKSCVL"
     gene            complement(5081..6874)
                     /locus_tag="PLS16_03500"
     CDS             complement(5081..6874)
                     /locus_tag="PLS16_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNJ60177.1"
                     /translation="MSTGATTKTLLKGGEFLIKETDFNDVFTPRDVNEEQNAIRQTAV
                     DFLETEVLTKPHLANEVDLETTKVLLEKMGELGLLGLAIPEEYGGFGQDFNTNSIVVE
                     AMAKARSFTLSWGAHVGIGTLPIVYFGNEAQKQKYLPKLASGEFKACYCLTEPSSGSD
                     ALGAKTRADLSADGTHYVLNGQKMWITNSGFADVFIVFAQVDGDKFTGFILEKDMPGL
                     TLNAEEKKMGIKGSSTRQVFLENVKVPKENLLGEIGKGHLIAFNILNIGRHKLGIGSV
                     GAAKVTLGHAAKYAMERQQFKTPIGKFGAIQYKIAEMAIRIYASESACYRASGDIDNL
                     EKILLNDGKAFQEAILGAADEYSIECAFLKVHGSEVLDYCVDEAVQVYGGMGFSEETE
                     VCGSYRDARINRIFEGTNEINRLLTVDMLIRKAMGGKLDLMSPAMAIQKELMSVPDFS
                     DADESTLNNEIKAVRNAKKAILMTAGAAVQKLMQKMKHEQEILMNIADMILETYAMES
                     SLLRVQKRIAEVGESEAELEIAILKTYVSDAMERVNLHGKHALQSFADGDELRIMLMG
                     LKRYTKTEVFNTKNARRKIAEKVLEEGRYCF"
     gene            complement(7010..7528)
                     /locus_tag="PLS16_03505"
     CDS             complement(7010..7528)
                     /locus_tag="PLS16_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNJ60178.1"
                     /translation="MAVQRIFEQNELSIIKIDLGEVEIKELLSDSQKEKLDKELTALG
                     FELIDDKKNRLTEQIKTIIIQLVHHQDNEMKNNLSEILSKELYHDYNYLSNLFSEVEN
                     TTIEKYFIAQKIERVKELLVYDELSLSEIADKLNYSSVAYLSNQFKKITGYTPSKFKQ
                     LESNNRKPLDLV"
     gene            complement(7597..9315)
                     /locus_tag="PLS16_03510"
     CDS             complement(7597..9315)
                     /locus_tag="PLS16_03510"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ60179.1"
                     /translation="MLVPFLQLIFKADAMKEYTYPIWNQKVSYQSYFTDWFTYFFTSI
                     INNEGKITALGFICLMTLIVFLLKNLSRYLALYVLAPIKTGMASRIRAQLFKKITYLD
                     SRYFLHNKKGELTTKLTHDIQEIEYGILFFLEVVLREPIMILITFVVMLLISIKLTLF
                     VLIVLPFSGYLIGKIGKQLKKTSLKAQSIQGNIATSIDEMMSNNQAIKSNNIESSIIA
                     KFNQINKIYYKLNTWMLRRRDLSSPLSEFLGMVVVLIVLFFGGSMILKQQSNLEAELF
                     ITYIVIFSQIIQPAKVFSNAFYFIQKGMASLERVEEVLNEKNEIEEQINGKLIVNFSE
                     SIIFKQVDFAYEQQLVLNQLSFEIPKGKRVALVGVSGSGKTTITKLLMRFYDVNKGKV
                     LLDNIEIKDIELKALRNLIAWVPQQNFLFNDTIAYNIALSENVDVKKMQMALEMANLQ
                     DFISHQSNGIETIVGDNGIKLSGGEQQRIAIARAFYKDAPIVILDEATAHLDSQNENA
                     IQNAIHKLMQDRTVLIIAHRLSTIQSCDKIIVLEKGKIIEQGNHQELMEKRATYYNLV
                     QMQQMQ"
     gene            9467..9865
                     /gene="rbfA"
                     /locus_tag="PLS16_03515"
     CDS             9467..9865
                     /gene="rbfA"
                     /locus_tag="PLS16_03515"
                     /inference="COORDINATES: protein motif:HMM:NF014130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNJ60180.1"
                     /translation="MESKRQKQVSKLIQITMSDVFQRDLQELTQNAMVTVISVHITPD
                     LFIARSYLSIFNHPNPTALIQLINDHKKQIRGLVGNKIRNKMRTIPELEFYLDDTLDE
                     VFKIEKLLQEVKAKDEFIAKNREDFEETDA"
     gene            9862..11100
                     /locus_tag="PLS16_03520"
     CDS             9862..11100
                     /locus_tag="PLS16_03520"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNJ60181.1"
                     /translation="MNLSLLIAKRYLFSKKKTNAVNIISGVSAMAIGVGAMSLVLVLS
                     VFNGLEGLVKSLYTVFYPEVLIVPKQAKTFEITDDIETILKSNSNIAVFSYTLEENAL
                     LEYNGQQYISTIKGIDDNYFKVVENFDKYIKEGTKNIKVNGTEFAILGAGIAYYLNIN
                     TAESIEPIGIYMPKKTAKSLSNVENAFQKRYINSCGMFAISDEFDVKYSIVPLDFFQE
                     LTEDYSQASKIEIKLKDNKKDKLTIKELSKKLGDNFTLKTRYEQNEVLYKVLQTEKWI
                     VFAILSAILIIASFNIIGALSMLILDKRKDMAVLTALGYSKENTHKLFLVEGMLLSLL
                     GANIGIIIGIILLLIQKYIGIIPMPGNTFVVEFYPVDIHILDIITVFILVAIISFIAT
                     WFPAKKAAENIEKEYLAYLN"
     gene            complement(11167..11586)
                     /locus_tag="PLS16_03525"
     CDS             complement(11167..11586)
                     /locus_tag="PLS16_03525"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNJ60182.1"
                     /translation="MKNITIISLCIVLLCAFVAPSSDYKGVQFQNINFNQALAKAKQE
                     NKFVFMNVYAVWCGPCKLLKKNTFTSEKVGAAVNKSFVSIDVDAEKGEGISLSKKYEV
                     KAHPLILIINPEGKVVKRILGYRTDAQLLSELKGLLK"
     gene            11801..12793
                     /locus_tag="PLS16_03530"
     CDS             11801..12793
                     /locus_tag="PLS16_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNJ60183.1"
                     /translation="MESFDIKALTEKIQQDSAFLDLLNREISKNIVGQKYMIERLVIG
                     LLTHGHILLEGVPGLAKTLAIKSLSDAIHTKFSRIQFTPDLLPADILGTMIFNQAKGN
                     FEVKKGPIFSNFILADEINRAPAKVQSALLQAMQERSVTIGDTVYKLEEPFLVMATQN
                     PIEQEGTYNLPEAQVDRFMLKVKIGYPEYEEERQIMRQNVQGDTSNIMPVVQPSDIIT
                     ARETVRKVYMDEKIEKYILDIVFATRNPSQYKLDNLKHLIGYGASPRASISMAMAAKA
                     YAFIKKRGYVIPEDVRAICVDVLRHRIGVTYEAEAENIYQEEIINTIVNKVEVP"
     gene            12874..13737
                     /locus_tag="PLS16_03535"
     CDS             12874..13737
                     /locus_tag="PLS16_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNJ60184.1"
                     /translation="MEVSDLLKKVRKIEIKTRGLSNQVFSGEYHSKFKGQGMSFSEIR
                     EYQYGDDIKNIDWNVTARYQTPFIKVFEEERELTVMLLVDVSQSTFIGTLNQTKREMI
                     AELCAVIAFSATQNNDKVGLVFFSNQTELFLPPKKGKHQILRIIRELLNFEPKQNTTN
                     ISSALEGFNNKMKRRSIVFVLSDFMNDGYFQALSHLARRHDVIGIKINDDLDEKIPEI
                     GLLQIKDVETGDIAWIDTNNHDWQQAYLNDYQKYTKYFEESFGKSGADKLSLKSNQKY
                     IHTLINFFKKR"
     gene            13737..14705
                     /locus_tag="PLS16_03540"
     CDS             13737..14705
                     /locus_tag="PLS16_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60185.1"
                     /translation="MLNISSILLRISKTIIFIGLIFIAFISNAQQVSANTKKKNYTIG
                     DWVPIDLTVTAEINNKVFFPNFKKVDSSANGNIEIVNASEIDTVKEGINYRYHQLVNF
                     ICFDTGKILFQPFPIIVSNGAKLDTLYTDAVLVHVAGVAIDTTKDIKPIKGPIKVPYT
                     FKELLPYFLIGLAILILLAFLIWFFKFRKKKAEPIDLKYLLPPDIWATQALDKVEQKQ
                     LWQHGETKLYYSELSDILRNYIELRFKVAAMEQTTDEIMQSLHRGMLKQKLKQPINEF
                     LNLSDFVKFAKAQPDINDNTNAIKTIRIFIDQTKPTNSETKDNNPQ"
     gene            14715..15722
                     /locus_tag="PLS16_03545"
     CDS             14715..15722
                     /locus_tag="PLS16_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNJ60186.1"
                     /translation="MSFFPTYHFASPQYFLLLLLLPILIYWYFIRKQSRFATFSVPNI
                     STNLLQQKTLKTTLNKYLPILHLLAFSFVVIALARPQTNLSDSEINTEGIDIVMALDI
                     SGSMLARDFKPDRLEAAKKLSMDFIKERKNDRIGLVVFSGESFTQCPITIDHQVLLNL
                     FKDIKSGIIEDGTAIGMGLATAVARLKNSQSKTKIIILLTDGVNNMGYIDPETSIQIA
                     KTNGIRVYTIGVGKRGMAPYPVQDQNGKIFYQNMPVEIDEVLLKKTAQETGGVYYRAT
                     SNNDLKAIYSNIDKLEKSKIKISNYAMKAERYHWFLLIAIIFLLSHFILRNTYLKTIT
                     N"
     gene            15722..16720
                     /locus_tag="PLS16_03550"
     CDS             15722..16720
                     /locus_tag="PLS16_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNJ60187.1"
                     /translation="MLQFAHITNLWLLVSVPLSILIYWIWYQKKKAILNQLADKRITN
                     ILLPNRNPQKTHLKFGLYLLSLICFILALAQPQLGTKQEKIKRKGIDVVIALDLSNSM
                     LAQDIAPNRLQKSKLFIQELLKKLDGDRVGLVIFAGKAYTQMPLTVDYSSMLMYLRSI
                     NTHSIPVQGTALAEALRQSETLFDKKDKKHKAVILITDGEDHEENAIDLAEELASTGV
                     KITTIGAGTKQGGKIPIIDAHGNTIDFKKDAEGNEINTALNENMMQALAKAGKGNYYI
                     LDNAKTVADLVSKDISKIETKTINEKVFTNFVEQFQYFLLIGLIFLLINLFIVYRK"
     gene            16717..17508
                     /locus_tag="PLS16_03555"
     CDS             16717..17508
                     /locus_tag="PLS16_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ60188.1"
                     /translation="MKKWLIKIKDFVARNKKTIAIITVLNYTIKAAIILILLLKGKEA
                     KAQSVNTFIRNGNQFYKEQKYNQATEQYASALQKEPNNSIAIFNQAAAMYQLGEYEKA
                     TTYFDGLAKQSKNHEMMSKSFHNLGNAFYKKEDYEKSVAAYKQALKLNPKDVDTKYNL
                     MMAMNKLKNNQNQQNKENQQQQNKDQQKNQDKQQPKEQNKNQPTQNKEQQQSASKPQQ
                     DKKQGMSKEEADRLLEALGNEENKTQQKIGKQKGEPQQGKVKKDW"
     gene            complement(17811..18434)
                     /locus_tag="PLS16_03560"
     CDS             complement(17811..18434)
                     /locus_tag="PLS16_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60189.1"
                     /translation="MGKLNELSITIGKDNKAEIVFDSLFPNFLEVEYSKPTEYIQKYW
                     DAYSKHSERNNNLNGKVFEYILATLCVRENILPIYMSAKVAFVPNVIYDIMFYTTERG
                     PICWSVKTSLRERYKQADLEAIALKYVHRKGLSFLITLEENEARSVKAKIKSGDVIGL
                     DNVIVATSEEFNELILELKTFEFSEPPTVKVIESNQIITEDKVSRIK"
     gene            complement(18436..19722)
                     /locus_tag="PLS16_03565"
     CDS             complement(18436..19722)
                     /locus_tag="PLS16_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HNJ60190.1"
                     /translation="MRERILEMNLKEKISLEIFGDKQFRIKLAEPENNQEAVLTHYLH
                     NSKNGVSKFYKEGALAFTKEILEYKYPDEKITSQVAEEALQYGLFDTFQIPFSPLENP
                     KFKFIDLFAGIGGFRLALQNLGGKCVFTSEWDKDAKKTYKANFGETPFGDITKEETKS
                     YIPDGFDVLCAGFPCQAFSIAGKRGGFEDTRGTLFFDVAEIIKRKQPQAIFLENVKGL
                     RSHNGGKTLATILNVLRNDLGYFVPEPEIINAKDFGVPQNRERIYIVGFHTSTGIDEF
                     TYPKPLHKEVTFADIKEETVPPTKYFLSTQYVQTLENHKARHENKGNGFGYAIIPDDG
                     ISNAIVVGGMGRERNLVLDHRITDFTPTTHIKGEVNRQGIRKMTPREWARLQGFPDNY
                     LIPVADASAYKQFGNSVAVPAIQATAREILKVINKL"
     gene            20017..21972
                     /gene="acs"
                     /locus_tag="PLS16_03570"
     CDS             20017..21972
                     /gene="acs"
                     /locus_tag="PLS16_03570"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNJ60191.1"
                     /translation="MYPYQIKTLENYHKAYKDSVEQPEKFWFEIAQHFKWKKQPTLEN
                     TLHWNFKEPKIEWFRDGTLNITENCLDRHLEKNGNTPALIFEPNAPEDKTITLTYKEL
                     HHKVCQFAHVLKNNGVKKGDRVCIYMGMVPELSIAMLACARIGAIHSIIFGGFSANSI
                     SSRLEDTQAEFVITQDGSYRGNKEIELKKIMDEALENSALVKKVIVFRHTNNNCKMKD
                     GRDMLWQDEITKVEAQGNPDFPAVEMNAEDPLYILYTSGSTGKPKGVVHTCGGYMVYT
                     NYTFVNVFQYQPGDIFFCTADIGWVTGHSYIVYGPLSAGATSLMFEGIPTFPDAGRFW
                     DIVDKYKVNILYTAPTAIRSLMAYGDSIFDGKDLSSLKVLGSVGEPINEEAWHWYYDK
                     VGKGRCPIVDTWWQTENGGILISGLAGITPMKAAHASFPLPGIQPILMDEKATVIEGS
                     DASKSQLPTETISGNLCIQFPWPGMLRTTYRDHERCRLNYFSAYDDKYFTGDGCLRDT
                     EGNYRITGRVDDVLNVSGHRIGTAEVENAINEHPLIVESAVVGYPHDIKGQGIYAYII
                     LQQSSENFDAQSIKQEVNQVVTKIIGAIAKPDKIQIVKGLPKTRSGKIMRRILRKIAE
                     GDTSNLGDTSALLEPAIVDSIKEGKVL"
     gene            22044..22871
                     /locus_tag="PLS16_03575"
     CDS             22044..22871
                     /locus_tag="PLS16_03575"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNJ60192.1"
                     /translation="MKNKRENFIKSLLDKSDLRDYIRVFPNKVGSEGYDAWGFNIKSV
                     KGTIRLAKILYENYFRVEVFGLENIPKEGRVLIIPNHSGQLPIDGVMVGYAMLTNPNG
                     PRAPKAMAERFFPTLPFIGNWINSVGGVIGDTVNCERMLDNDEAIIVFPEGVRGSGKT
                     FDKRYQLQRFGNGFMHLAIKHKTPIIPVGIVGFEESIISIADIKGLAKILAVPYIPLA
                     LPAILPSKVYIHFGKPMYFEDGEFTEAEVKEKVDAVKEEIKQLIKEGLQKRKSIFQK"
     gene            22874..23806
                     /locus_tag="PLS16_03580"
     CDS             22874..23806
                     /locus_tag="PLS16_03580"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ60193.1"
                     /translation="MENRKKIMITGAAGALAKKVIEQLKKDYKIIAVDFRSKVDLGIP
                     LESYKVDFNKRIFEDIFREHQFEGIIHLGRIGASESNRHARYNANVIGTRKLFDLAKK
                     YGVKQTIVLSTYFVYGASPYNPSLLDEKTPLKASELTMDLVDSVELENLANIYLYKYP
                     ELNITILRPCNIAGPGVRNTFSQLLSEKRAPVLWGFSPLMQFIHIDDMQEAICTAYKQ
                     NKPGVYNIAPADYIAFQDAVKEAGCSTIQIPSIPPSLTDRIARMLNWKTLPPFLINYF
                     KYPVIIDGSLFEKTFNFKPKRTLKEILSYYKNLK"
     gene            23862..>24508
                     /locus_tag="PLS16_03585"
     CDS             23862..>24508
                     /locus_tag="PLS16_03585"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNJ60194.1"
                     /translation="MKKNSSLLAIFIFILLIVVSCAVNPVTGKKQLSFMSEEQEKAMG
                     LESDPAVIAQFGVYPNDTLQKFIQSEGDAMVKVSHRPDLKFTFRLLDANVINAFAVPG
                     GYVYFTREIMAYFNNEAQFAGVLGHEIGHITARHSAQQYTKQMITQVALVGGMIFSEK
                     LRNMGEQAMQGMQLLFLKFSRDDETQADNLGVQYSSLVGYDAVEMAKFFKTLERVS"
BASE COUNT         8781 a         3863 c         3528 g         8336 t
ORIGIN      
        1 gtcagcgagt ttcttcgttg aagttcctcc cttatttcct gctgtgcttt caacaatctt
       61 aacatttggc tcttgctcaa tgttatcttc gattgtgagt tttgattctg cgtgtctgtg
      121 ttcggcttca tcttgtgcta attttccgtc taagttatca aaatttaacg aatgttgcaa
      181 attatttttt ggagcttctt tttttgtact gcttttagtg ttttctgtac ctcctttctc
      241 cagtttaagt tgctcctcta aggctgttaa ttttttcttt tcctctgctt aatttaatac
      301 ctttctaaaa aattatctaa aacgatttct gtccataatt ataataaaat gtttcaatag
      361 attaacgact ccctatctat gggtaaatgt ctttctctaa aattggctca cacaattaga
      421 taaaaataaa aagcgtaaaa ataaaatagc ctgaataaga tagttgcaaa ttgaataaat
      481 attagtcaaa attttgttat aattacgaaa ggtaaattac ttaaatttat cattccaaaa
      541 agactattat gaaattaaag cttgcacttt tatctctatg tattctgttt gtactttttg
      601 caaacgccaa tatggcatca cctattcgac ctggcacaaa aactagcacc gtattttcta
      661 gcaaggatat tgatattctg tcggagtcta tccacattaa gattgctcca gattttaaaa
      721 cagcaaaatt cactgtagtg tacaatattc aaagtaatag ttctggtaaa caaataccgc
      781 ttttatttta tgcccaagcc tataacgata aatttatagt tctattagat gatcatcaag
      841 ttcctatcca agatattcca gcagatttta tcccttcaac ttcaataaat ggatttaaaa
      901 ataattttga taaagttgaa attacttgga ataaagatta tcaacctacc tatatgctgc
      961 aagatttaaa atattttgaa actgacatct ctaaaggaaa ccatagaatt aaggtaagct
     1021 atactgcaaa tgtttgggct gatgtttctg gttggacaaa aaaatacagt ttctattatt
     1081 ctttaacacc agctaaattt tggaaatcat ttggaacttt agatattagc ttagagcaag
     1141 caggaaatat aaaacaacta acaacaaaca ttggactatc tgaagaaaaa aaatttcaac
     1201 aaaagaataa ttggcatttt aaccaattgc ccgatgaata tttagaattc tcctacactc
     1261 caaatcctaa taaattagct tctattttaa tattaattga accatctgga attgcacttt
     1321 tagttggttt aattctttta gtattacatt taattttaat cgtactctat aggaaaaact
     1381 atcctaataa aaaacattca atagtggtca gcttaggaag tatattagtg ccacttttgt
     1441 ttttattaag ctatatctat tcttttataa taattgatga tattataggt gcagatgctg
     1501 gaaaatctca tggctattct gcgattttgg ctttaatgct ttatccagtt ttactaccta
     1561 tttatgggct ttttatgtgg cttatagaca aaattattaa aaggaaaata agtaaataaa
     1621 aacattataa actcaacaaa tctgcataag cagaatatgt ataactctta gattctgcgt
     1681 taataacaaa aggtgttttt atatctaata tttcttgcaa caatgcaaat aatctttgtt
     1741 caaattcatc taaaattgcc ttaggaattg ggtttccatc attaagatat tgaatgcctc
     1801 cttgaagttt tttggctaaa taaaaaccaa cgcttatcga tacatctttt gcatgaacat
     1861 ctatggattt gctatataac caagcataca tcaagccttg aaaagtttca gggaatccac
     1921 cattatccaa tccaaataat ttattaaaaa acttatctat ttcagattct tctggcttat
     1981 tattttttgt tttacttttt ttgttttcat caggaaatgc caatagtttt acttctccag
     2041 ttttgtaatc taaaattctg atggaattat caattctatc aactctatct aaatttccat
     2101 taattttaat tttaaagtca tgtaagtaaa cctctgtact ataattctgt tctaatccaa
     2161 caatcttaaa atcttcttca ctatcatttt ttaaaatatc taaaactaat tttttaatca
     2221 cttttttatc tagcactcgc tttcctgtaa aataatttag aggaacaatt ttagggtctg
     2281 aaatcgcttg attaaaataa gtatcaatat cttttaattc aacactattt tgaaaatcgc
     2341 tataatacaa ttctaaaatt ttatgtaaaa tagttccgaa ataggcacta tcaaaatttg
     2401 catataattc ttttggttcg ctaattccaa caacatactt caagtaaaat tgaacaggac
     2461 aacttaaata caatttcaat gaacttggtg aatatattcg ctcttttagc ttatcaataa
     2521 tagcctttgt tttattaaat tcaatagctt tattgatttt tttatcatca ttgtttttca
     2581 ttaaaacaga ctgttgtatt atggtttcag tttcaaattc agattcaatt tgtctaatga
     2641 atcgactttt ttcttcttca ttatcaatcg atgaattatt ataaatcaat gtaatatttt
     2701 ttccacgttt caataatcta taaaaatgat atgcatttat tccgtcaaat tggtcgaaag
     2761 taggtagatg aaaacttttt ctaagattat aaggaataaa tgaattggtt ctatttgttc
     2821 ctggcataat cgaatcattt aaacttagaa tatagatatt atcaaaatct tgcaacctcg
     2881 tttctagaaa tcccataatc tgaattttac tatctagttt tgtttcataa gctatgcttt
     2941 ggttacccac atacatattc aaaattacaa gtaaatcctc agcagactga atgatatgtt
     3001 gttgagttgt aaattgaata gtagaaacat gctccatcaa ggtctgtttt aaatactgtt
     3061 ctgcattgga caattgtatt aggttcaata aattttgaaa aatagttgaa acattaaacg
     3121 gtttagccat caattgatac aaatcatctg aaattaaagt ttgtaccaat gacaaagaaa
     3181 taatttgact attatacaaa tgcttaaaca actcgcctct ttttggtatt tgtttttcta
     3241 ataatcctga aataaaaaat ggcaatgcag tttctccttt tatatatgta ttttcatcag
     3301 atagcaccat tgaattaagt aaactaatat aggtactcag caattcaaaa acactcatct
     3361 tatttatgct taatcccatt gtaatattgg tagcttcagc attaaaaatc ggcaaacaat
     3421 aatccattag attttcatca cacaaaacaa tacaattatt ttcatctata ttcatattac
     3481 tccgaacata atgtacttgt ccaacatttg tcggcattgc tacaacctca atacttttat
     3541 tcagtgtttt atctgaaatc caatggtgtt gctcgccatt atattcttga taatattttc
     3601 taaggaaatt accagcttcg tggtattcat tttttacaaa tgcttcatca atatcccacc
     3661 aagtattaac tgaatattga ctgattaaag tatctaaaag ttttttctca caagttgtaa
     3721 aggcattaaa tccacaaaaa tgaattttca aatagggcaa ttgcttttga ttagactcaa
     3781 tttcttttaa caataaccga aatgcttttc cctcataaat aatattttta tctaataatc
     3841 tttgtccaaa atgattataa atttcgccta agtatttcca agtctgaata aaatcttcaa
     3901 agaaagctcc ttgtttttca ttgtgaatat ttgtaaaaaa gctttccaaa tattccttca
     3961 caatttcatc aacattgaaa tgagcttcga cttctttgtg tgccaataaa acatcaaaaa
     4021 aatactttgt atctaccaaa tatttatcaa tttcatcaaa atctttcagt actaattttc
     4081 cccaacctaa gaattgatga aaaggctgtg gagtttgagt tacagcttca ttcacaaaat
     4141 ataattcttg caataaagta tattcatcaa ctacattata attagaattt tcaatgaaaa
     4201 acgattgaat cgtttgcact ttagggaaaa caaaaatttc attttcaaaa taattttgca
     4261 acaaatcatt gaataaagca actgcacgtt tactaggaaa caaaaacaat tgattttgta
     4321 agttttcgca actctctgta tgttttatat gctcaacaat ttgttcaata aatttcatct
     4381 gtaatactga ttagaaatga caatatacaa aatcttaatt taaagaactg aaaaatcaaa
     4441 aatagtttat aaaagtagta cagaaaagaa aagaggctat ccaaggcatt tcggacagcc
     4501 tcttcaatta gttattaatc tagttatagt acacaggact ttggagtttt tgacaatact
     4561 atttttacct tatctgagct aaaagtacct tctatcgata atttggaatt agattctgaa
     4621 aacattacac tggtaaaaac aattttcggt gcaccgtcaa gagtaataga cattccaatt
     4681 ccattttcat caacagaata aactccagat tgaagtgaag tataagaagc tatcattgtg
     4741 ccatctggac taaaaagaaa aaaatgtgac ttattgaaac aatccgcttc aagtactcca
     4801 tttctatatc gctcttgata ataccatctt cccgtagtta aaagtttgac tacttcttct
     4861 ttggcactta gttttgtatc ttcttttgta caagacggca ctatacttac catgcttatg
     4921 aggattagtg catataacaa tacttttttc ataactttca atttaattac tttcaaattt
     4981 aaacaataaa ccctttattg tcaatattta ttttcaggga attatacata aaaaaaggtt
     5041 cttcatagaa gaaccttttt tttaaaatta ttaatggtta ttagaaacaa tatcttcctt
     5101 cctctaatac tttttctgca atttttcttc ttgcattttt agtattgaaa acttcagttt
     5161 tagtgtaacg ttttaagccc atcaacataa ttcttaattc atcgccatca gcaaatgatt
     5221 gcaaagcatg ttttccgtgt aaattcactc tttccattgc atcacttaca taagttttta
     5281 agatagcaat ttccaactcc gcttcacttt ctccaacttc agcaattctt ttttgaactc
     5341 ttaataaaga agactccatt gcataagttt ctaaaatcat atctgcaata ttcatcagaa
     5401 tttcttgctc gtgtttcatt ttttgcatca acttttgaac agcagcacct gcagtcatca
     5461 aaattgcttt cttagcattt cttactgctt taatttcatt attcaaagta ctttcatcag
     5521 catcagagaa atcaggaacg ctcatcaatt ctttttgaat tgccatagct ggactcatta
     5581 aatctaactt tccacccatt gcttttctaa tcaacatatc tactgtaagc aaacgattaa
     5641 tttcgttggt accttcaaat attctattaa ttctagcatc tctatatgaa ccacaaactt
     5701 ctgtttcttc tgaaaagccc attccaccat aaacttgtac tgcttcatct acacaataat
     5761 ctaaaacttc tgagccatgc acttttagga atgcacactc aatagaatat tcatcagcag
     5821 cacctaaaat cgcttcttgg aatgctttac catcattcaa taatattttt tctaaattat
     5881 caatatctcc agatgcacga taacaggcac tttcagaggc ataaatacga attgccattt
     5941 ctgcaatttt atattgaatc gcaccaaatt taccaattgg cgttttaaat tgctgacgct
     6001 ccatagcata ttttgcagcg tgtccaagtg taactttagc agcaccaact gagccaattc
     6061 ctaatttatg acgaccaata tttagaatat taaatgcaat taaatgtcct tttccaatct
     6121 cgcctaataa gttttcttta ggtactttta cattttccaa aaacacttgt cttgtcgaag
     6181 agcctttaat acccattttc ttttcttcag cattcaaagt caatcctggc atatctttct
     6241 ctaaaataaa tccagtaaat ttatcgccat caacttgagc aaaaacaata aatacatctg
     6301 cgaaaccaga attggtaatc cacatttttt gtccatttaa aacgtaatgt gtcccatcgg
     6361 cagacaaatc agctctagtt ttagcaccta aggcatcaga gccagaactt ggctctgtta
     6421 agcaataaca agctttaaat tctccagatg ccaactttgg taaatatttt tgtttttgtg
     6481 cttcatttcc aaaataaaca attggcaaag ttccaattcc tacgtgtgct ccccaagaaa
     6541 gtgtaaatga acgagctttt gccattgctt ctacgacaat agaattggta ttaaaatctt
     6601 gaccaaagcc gccgtattcc tcaggaattg ccaaacctaa caaacctaat tcacccattt
     6661 tttctaataa aactttggtt gtttctaaat ctacttcatt tgctaaatgt ggtttcgtta
     6721 aaacttctgt ttctaaaaaa tctactgcag tttgtcttat ggcattttgt tcctcgttta
     6781 catctcgtgg tgtaaacaca tcattaaaat ctgtttcttt gattaaaaat tcacctcctt
     6841 ttaataatgt tttagttgtt gctccagtac tcatcgtttt taatttgttt taattaatta
     6901 atgtttaaat atttattact aacaaaaata caatgcatgc attgaattac caaatgtttt
     6961 aaaatatttt ttagtttctg attttcaaca aaaaagggat tataaaaaac taaactaaat
     7021 ctaatggttt tctattgtta gattctaatt gtttgaattt gcttggcgtg tatccagtaa
     7081 tttttttgaa ttgattgctc aggtaagcaa cgcttgaata gtttaattta tcagcaattt
     7141 cgcttaatga taattcatca taaaccaata attctttaac tctttcaatt ttttgagcaa
     7201 taaaatactt ctcaatcgtt gtattttcta cttctgaaaa taaattagac aaataattat
     7261 aatcgtgata taattcttta ctcaaaatct ctgataaatt atttttcatc tcattatctt
     7321 gatgatgaac caattgaatg ataatagttt ttatctgttc ggtcaatcta tttttcttat
     7381 catcaattag ttcaaaacct aaagcggtaa gctctttatc tagcttttct ttttgactat
     7441 cagacaatag ttcttttatt tcaacttctc ctaaatcaat ttttataata gataattcat
     7501 tttgttcaaa tatgcgttgc acagccatta tacaacgatt acaaaccata ttttttataa
     7561 aaagtgttgt catattaatc aaataaaaca gaataattat tgcatttgtt gcatttgtac
     7621 taaattataa taggtagctc gtttttccat taattcctga tgattacctt gttctataat
     7681 tttacctttt tccaaaacaa taattttatc gcaagattgt atggtggaca aacgatgagc
     7741 aatgataagc actgttctat cttgcattaa cttatgaata gcattttgaa tagcattttc
     7801 attttgacta tctaaatgtg ctgtcgcttc atcaagaatt acaatcggtg catctttgta
     7861 aaaagcacgt gcaatagcaa tccgttgttg ttctcctcca cttaatttaa taccattatc
     7921 tccaacaatc gtttcaattc cattggattg atgactaata aaatcttgca aatttgccat
     7981 ttccagagcc atttgcattt tttttacatc tacattttca gaaagtgcaa tattataggc
     8041 aattgtatca ttaaataaaa aattctgttg tggtacccaa gctattaagt tacgcaaagc
     8101 ttttaattca atgtctttaa tttcaatatt atccaataaa actttgcctt tattgacatc
     8161 ataaaaacgc atcaacaact tagtaatcgt cgtttttcca gagccagaaa cgccaaccaa
     8221 cgcaactctt tttcctttag gaatctcaaa tgaaagttgg tttaaaacaa gctgctgttc
     8281 ataagcaaaa tctacttgtt taaaaataat actttcagaa aagtttacaa ttaatttccc
     8341 atttatttgc tcttcaattt catttttttc attcaatact tcctccactc tttctaaaga
     8401 tgccatacct ttttgaataa aataaaaagc attagaaaat acttttgcag gttgaataat
     8461 ttgagaaaaa atcacaatat aggtaatgaa taattctgct tccaaatttg attgttgctt
     8521 taaaatcata ctaccaccaa aaaacaatac aattaataca acgaccattc ctaaaaactc
     8581 agaaagtggc gaagacaaat ctcttcgacg caacatccac gtatttaatt tataataaat
     8641 tttattaatc tgattaaatt ttgcaataat tgaactttct atattattcg attttatcgc
     8701 ttgattatta ctcatcatct catcaattga agtagcaata ttcccttgta tagattgtgc
     8761 ttttaatgaa gtttttttca attgcttccc aatttttccg attaaataac cagaaaaagg
     8821 caatactatc aaaacaaata aagttaattt tatactaatc aataacatca ctacaaatgt
     8881 tatcaatatc atgatgggtt cacgtaaaac aacttctaaa aagaacaaga tgccatattc
     8941 aatttcttgt atatcatggg ttaattttgt ggtaagttca ccttttttat tgtgtagaaa
     9001 ataacgacta tctaaataag taatcttttt aaataattgt gctctaatcc tagatgccat
     9061 acctgttttt attggtgcca aaacatacaa agcgagatac cttgacagat tttttaataa
     9121 gaaaactata agcgtcatca aacaaataaa gcctaaggct gtaattttac cttcgttatt
     9181 tattattgaa gtaaaaaaat aagtaaacca atctgtaaaa taggattgat aagaaacttt
     9241 ttgattccaa ataggatatg tatattcttt catcgcatct gccttaaaaa tcaattgtaa
     9301 aaaaggcact aacatcaaaa aggaaaaaat tgaaaaaata gcagttaaag cattgagtat
     9361 tagattcaat atcaatctaa aataatatcc attcaaatac ttatttattt tgtaaaacgc
     9421 tttcacttgt aatacacaaa gatattattt acatttgtta tattgtatgg aaagtaaaag
     9481 acaaaaacaa gtaagcaaac taattcaaat taccatgagt gatgtttttc agagagattt
     9541 gcaagaactc actcaaaatg caatggtaac agtcatcagt gtacatataa cacctgattt
     9601 atttattgca agaagctact tgagtatttt taatcaccca aatccgactg cattaattca
     9661 gcttattaat gaccataaaa aacaaattag aggattagtt ggtaataaaa tcagaaataa
     9721 aatgcgtacc attccagaat tggaatttta tttagatgat acattagatg aagtattcaa
     9781 aattgaaaaa ttacttcaag aagtaaaagc aaaagatgag tttattgcta aaaatcgtga
     9841 agatttcgaa gaaactgatg catgaattta tctttattaa tagcaaaaag atacttattt
     9901 tctaagaaga aaaccaatgc tgtcaatatc atttcgggcg taagtgctat ggctattggc
     9961 gttggtgcaa tgtccttagt tttagtgctt tctgttttta atggattaga aggtttagta
    10021 aaatctcttt atacagtttt ttatcccgaa gtacttattg ttccaaaaca agctaaaacc
    10081 tttgaaatta cagatgatat agaaacaatt ctaaaatcaa atagcaatat tgctgttttc
    10141 tcttatacat tggaggaaaa tgccttatta gaatacaatg gacaacaata tatttcaacc
    10201 ataaaaggca ttgatgataa ttattttaag gtagtcgaaa attttgataa atacatcaaa
    10261 gaaggtacaa aaaacataaa agtaaacgga acagaatttg ccattttggg tgctggtata
    10321 gcctattact tgaatataaa tacagcagaa tccattgaac caataggaat ttatatgcca
    10381 aaaaaaacag caaaatcttt atctaatgtc gaaaatgcat ttcaaaaaag atatatcaat
    10441 agttgcggta tgtttgcaat ttcagatgaa tttgatgtaa aatactctat cgttccttta
    10501 gatttttttc aagaattgac agaagattat agtcaagcat caaaaattga aatcaaacta
    10561 aaagacaata aaaaagacaa actaacgata aaagaattaa gcaaaaaact tggagataat
    10621 tttactttaa aaacaagata tgagcaaaac gaagttttgt ataaagtatt acaaacagaa
    10681 aaatggattg tttttgctat actatcagcc atcttaatta ttgcttcatt caatattatc
    10741 ggtgcattgt ccatgctcat tttagataaa agaaaagata tggcagtttt gactgcatta
    10801 ggatattcta aagaaaacac acataaacta tttttagtag aaggcatgtt attgtcttta
    10861 ttaggtgcaa atatcggaat tattataggt ataattttat tgctcattca aaaatatatt
    10921 ggaattattc ctatgcctgg caatactttt gttgttgaat tttatcctgt agatatacat
    10981 atcttggata ttataactgt atttatacta gtagcaatta ttagttttat tgcaacttgg
    11041 tttcctgcaa aaaaagctgc tgagaatatt gagaaagaat acctagcata cttaaattaa
    11101 acctaaactt tgtatatttt taatgaataa gatatctaag ctcctctaac ttatggctac
    11161 aaaaaactat ttcaataagc ctttcaattc agacaataat tgagcatcgg ttctataacc
    11221 taatattctt ttcactactt taccttctgg attaataatt aaaataagcg gatgtgcttt
    11281 aacttcatat tttttagata aactaatacc ctctcctttt tcagcatcaa catcaatact
    11341 tacaaagctc ttattaaccg cagctcctac tttttctgat gtaaaagtat tttttttaag
    11401 taatttacaa ggaccacacc aaacggcata aacattcata aaaacaaatt tattttcttg
    11461 ctttgctttt gctaatgcct gattaaaatt aatattttga aactgcacgc ctttataatc
    11521 ggaacttgga gcaacaaatg cacaaagcaa aactatacat aaactaataa tagtaatatt
    11581 tttcatctct aaatctttaa taggataaat ttaaccgatt taaaaaggaa tagcaaaaat
    11641 atcactcttt ttaagatctg aaaactcagt agttttgaat atcatcaaaa ttaaaaaaca
    11701 aaaaaagcat aataaaaaca agctttaata tcttttaaca caatacgttt agacattaat
    11761 atttacaaag ttatttttgt acatataata agaaaatctc atggaatctt ttgatattaa
    11821 agctttaacc gaaaaaatcc aacaagacag tgcattcttg gatttgctaa acagagaaat
    11881 ttccaaaaac atcgttggac aaaaatatat gattgaacga ttggttattg gattgttaac
    11941 gcatggacat atcttattag aaggtgtacc tggtttggca aaaacattag ccattaaatc
    12001 attatctgat gcaattcata ccaaattcag tcgaattcaa tttacaccag atttattacc
    12061 tgctgatatt ttaggtacta tgatattcaa tcaagctaaa ggaaattttg aagttaaaaa
    12121 aggtccaata ttttcaaatt ttattttagc agatgaaatc aatcgtgcac cagcaaaagt
    12181 acagagtgca ttattacagg cgatgcaaga aagatcagta acaattggcg atacagtata
    12241 taaattagaa gagccatttt tagtaatggc aacacaaaat cctatcgaac aagaaggaac
    12301 ttataatttg ccagaagctc aagtagatag atttatgttg aaagtaaaaa ttggctatcc
    12361 tgaatatgaa gaagaaagac aaattatgcg tcagaatgta caaggcgata ctagcaatat
    12421 aatgcctgta gttcaacctt ctgatattat tactgcaaga gaaactgttc gtaaagttta
    12481 tatggatgaa aagattgaaa aatatatttt agatatagtt ttcgctacaa gaaatccaag
    12541 tcaatataaa cttgacaatt taaaacattt aattggttat ggtgcttctc cacgtgcaag
    12601 tatcagcatg gctatggctg caaaagctta tgcatttata aaaaaacgag gctatgttat
    12661 tcctgaagat gtacgtgcca tttgtgttga tgttttgcga cacagaattg gtgttactta
    12721 tgaagctgaa gccgaaaata tatatcaaga agaaattatc aatactattg taaataaggt
    12781 ggaagtgcct tagtttataa tcagtatttg ataaaaagta agtaataaac gataatatta
    12841 attcataagt cctagtattc tatcaagaaa aaaatggaag tatcagattt gctcaagaag
    12901 gtaagaaaaa ttgaaatcaa aactcgtggt ttgagtaatc aagtattttc tggagaatac
    12961 catagcaaat tcaaaggaca aggaatgtca ttttctgaaa tacgagagta tcaatatggc
    13021 gatgatatca aaaatattga ttggaatgtt actgcacgtt atcaaacacc atttataaaa
    13081 gtttttgaag aagaaaggga attaacggtc atgcttttag tagatgttag tcaatctact
    13141 tttattggca cactcaatca aaccaaaaga gagatgattg cagaattatg tgctgttatt
    13201 gcattttctg ctacacaaaa taacgacaaa gttggactgg ttttctttag taatcaaaca
    13261 gaattattct tgccacccaa aaaaggaaag catcagattc taagaatcat tcgtgaactt
    13321 ttgaattttg agccaaaaca aaatacgacc aatatttcaa gtgctttaga aggatttaat
    13381 aataagatga aaagacgaag tatcgttttt gtactttcag attttatgaa tgatgggtac
    13441 tttcaagcat taagtcattt ggcaagaaga catgatgtga ttggaattaa aattaatgat
    13501 gatttagatg agaaaattcc agaaattggt cttttacaaa ttaaagatgt agaaactggt
    13561 gatattgctt ggatagatac caacaatcac gattggcaac aagcttattt gaacgattat
    13621 caaaaataca caaagtactt tgaggaaagt tttggcaaat caggtgcaga taaattgagt
    13681 ttaaaaagta atcaaaaata cattcataca ttaatcaatt tctttaaaaa aagatagtgc
    13741 taaatatttc ttccatatta ttaagaatat ccaaaacaat aatatttatt ggcttgattt
    13801 ttattgcatt tatttcaaat gcacaacaag tatccgctaa tactaagaaa aagaactaca
    13861 ctattggaga ttgggtaccg attgacctta ctgttacagc tgaaataaat aataaagtat
    13921 tttttccaaa ctttaaaaaa gtagatagta gtgcaaatgg aaatattgaa atagtaaatg
    13981 cttcggaaat agacactgtc aaagaaggga tcaattatag ataccatcaa ttggtcaatt
    14041 ttatatgctt tgatactgga aaaattttat tccaaccttt tccaataatt gtttcaaatg
    14101 gtgcaaaatt ggatacactt tatactgacg ctgttttggt acacgttgct ggtgtagcta
    14161 ttgatacgac caaagatata aaaccgatta aaggccccat aaaagtacct tatacattca
    14221 aagaattact accctatttc ttaataggat tagctatttt aatactcctc gcatttttga
    14281 tttggttctt taaattcaga aaaaagaaag ctgagcctat tgacttaaaa tacctattgc
    14341 cgccagatat ttgggcaact caagcattag ataaagtcga acaaaaacaa ctttggcaac
    14401 atggcgaaac aaaattatat tactctgaat tatctgacat cttacgaaac tatatagagt
    14461 taagatttaa ggttgcagca atggaacaaa ctactgatga aattatgcaa tccttacatc
    14521 gtggtatgct caaacaaaaa ctaaaacaac ctattaatga atttttgaat ttaagtgatt
    14581 ttgtaaaatt tgcaaaagct caacctgata ttaatgataa tacaaatgcc ataaaaacaa
    14641 ttagaatatt tatagaccaa acaaaaccta ctaattccga aacgaaagat aataatccac
    14701 aatagaaaat ataaatgtca tttttcccaa catatcactt tgcaagtcca caatattttt
    14761 tattattgtt gttgttgcct attttgatat attggtattt tattagaaaa caaagtcgat
    14821 tcgctacctt ctcagttcct aacatttcaa caaatttatt acaacaaaaa acactaaaaa
    14881 ctactttaaa taaatactta cctattctac atttattggc attttcattc gtcgttattg
    14941 cattagcaag acctcaaacc aatttatcag acagcgaaat aaatacagaa ggaattgata
    15001 ttgttatggc tttggatatt tcaggaagta tgttagccag agattttaaa ccagatagat
    15061 tggaagctgc aaaaaaactg tctatggatt ttatcaaaga acgaaaaaat gacagaatcg
    15121 gtttggttgt attttcggga gaaagtttta cacaatgtcc aattaccata gaccatcaag
    15181 tattattgaa tttatttaaa gatataaaaa gtggcatcat tgaagatgga actgcaatcg
    15241 gaatgggctt ggcaactgct gtcgctcgtt taaagaatag tcaatcgaaa actaaaatta
    15301 taattctatt gacagatggc gttaataaca tgggctatat tgatcctgaa acttccattc
    15361 aaatagctaa aaccaatggc attcgtgttt atacgattgg agtaggaaaa agaggcatgg
    15421 caccttatcc agttcaagac caaaatggca agattttcta tcaaaatatg cccgtagaaa
    15481 ttgatgaagt cttattgaaa aaaacagctc aagaaacagg cggtgtttat tatagagcaa
    15541 ctagcaataa cgacttaaaa gcaatatatt caaatatcga caaattagaa aaatcaaaaa
    15601 taaaaatctc aaattatgct atgaaagctg agcgttatca ttggttttta ttgattgcta
    15661 tcatttttct tttaagtcat tttatactta gaaacactta tttaaaaacg attaccaact
    15721 aatgctacaa tttgcacata taacaaattt atggttattg gtaagtgtgc cattatctat
    15781 attaatctat tggatttggt atcaaaagaa aaaagccatt ctaaaccaat tagcagacaa
    15841 aagaataact aatattttat tacctaatag aaatccccaa aaaacacacc taaaatttgg
    15901 tttatattta ttgagtttaa tttgtttcat cttagcattg gcacaaccac aattaggtac
    15961 aaaacaagaa aaaataaaac gcaaaggaat tgatgttgtc atagcattag atttatccaa
    16021 tagtatgttg gcacaagata ttgctcccaa ccgtttacaa aaatcaaaat tatttattca
    16081 agaattatta aaaaaactag atggcgaccg agttggattg gtgatttttg caggaaaagc
    16141 ctatacacaa atgcctttaa cagtggatta ttcatctatg ttgatgtatt taagaagtat
    16201 caatacacac tctattccag ttcaaggaac agccttagcc gaagcattaa gacaatctga
    16261 aacattattc gacaaaaagg ataaaaaaca taaggctgta attttaatta cagatggaga
    16321 agatcatgaa gaaaatgcaa ttgatttagc tgaagaattg gcttcaactg gtgtcaaaat
    16381 taccacgatt ggagcaggaa caaaacaagg tggtaaaatt ccaataattg atgcacatgg
    16441 taataccatc gatttcaaaa aagatgctga aggaaatgaa atcaatactg cattaaatga
    16501 aaatatgatg caagctttgg caaaggctgg aaaaggaaat tattatattt tagataatgc
    16561 caaaactgtt gccgatttag tttcaaagga tatttcaaaa attgagacaa aaaccattaa
    16621 cgaaaaggtt ttcactaatt ttgtggaaca atttcaatat tttttattga taggtttaat
    16681 ctttttactc attaatttgt ttatagttta tagaaaatga aaaagtggct gattaaaata
    16741 aaagattttg ttgcaagaaa taagaaaacg attgcaatta ttaccgtatt aaattatact
    16801 ataaaagctg ctattattct aattttactt ctgaaaggaa aagaagcaaa agctcaaagc
    16861 gtcaatactt ttatcagaaa tggcaatcaa ttttataagg agcaaaagta caatcaagcg
    16921 actgaacaat atgcatcagc cttgcaaaaa gaacccaata atagtattgc aatttttaat
    16981 caagctgcag caatgtatca attgggggaa tatgaaaaag cgacaactta ttttgatggc
    17041 ttagctaaac aatcaaaaaa tcacgaaatg atgtcaaaat cttttcacaa tctaggcaat
    17101 gctttttata aaaaagaaga ttatgaaaag agcgtagctg cctataaaca agctttaaaa
    17161 ttaaacccta aagatgttga taccaaatac aatttgatga tggccatgaa taaattgaaa
    17221 aacaatcaaa atcaacaaaa taaggaaaac caacaacaac aaaataaaga tcaacagaaa
    17281 aatcaagata agcaacaacc taaagagcaa aacaaaaatc aaccaactca aaataaagaa
    17341 cagcaacaat ctgcttcaaa accacaacaa gataaaaaac aaggtatgag taaagaagaa
    17401 gccgaccgat tattggaagc attaggtaat gaagaaaata aaactcaaca aaaaataggt
    17461 aaacaaaaag gcgaaccaca acaaggaaaa gtcaaaaaag attggtagct attacgattt
    17521 tgcaaaacaa cagattttta gcactaattt taaaacgaaa ccgacactct aaacatacaa
    17581 tagagtgaat tttctacaag aattgctcga agatttcaat tttatcatca ttagagttca
    17641 tttcattttt aagtattatg ccaaaagttt tataaaatct tctctagaca tttttagaca
    17701 atattccatt tttttctgca ttttgagata tgaattccta taaaaatcct ctttgtaaaa
    17761 aacgtgcaaa aagattggaa tgaaaagtta gaacataggg aacgttcaat tcattttatt
    17821 ctgcttactt tatcttctgt tataatttga ttactttcta ttacttttac tgtcggtggc
    17881 tctgaaaatt caaatgtttt cagctccaga atcaattcat taaattcttc acttgttgcc
    17941 acaatcacat tatcaagtcc gattacatct ccacttttaa ttttagcttt cacgcttctt
    18001 gcttcatttt cctcaagtgt tatcaaaaaa ctcaatccct ttctatgcac atactttaaa
    18061 gctattgctt ctaagtcagc ttgtttatag cgttcacgta aacttgtttt tacactccaa
    18121 caaatcggac ctctttctgt tgtgtaaaac attatgtcat aaattacatt tggaacaaaa
    18181 gcaacttttg cactcatata aataggcaaa atattttcac gaacacaaag cgtagccaat
    18241 atatattcaa atacttttcc atttaggtta ttgtttcttt cagaatgttt ggaatatgcg
    18301 tcccaatatt tttgaatata ctcggttggt tttgagtatt caacttctaa aaaatttgga
    18361 aacaaactat caaaaactat ttcggcttta ttgtcctttc ctatcgttat gctcaattcg
    18421 tttaatttcc ccattttata gtttattaat tacttttaaa atttctcttg ctgttgcttg
    18481 tattgcaggg accgctaccg aattgccaaa ctgcttataa gcagaagcgt cagcaactgg
    18541 aattagataa ttgtctggaa aaccttgtaa tctcgcccat tctcttggtg tcatttttct
    18601 aattccttgg cggtttactt ctcctttgat atgtgttgtt ggtgtaaaat ctgtaattcg
    18661 gtggtctaaa accaaattcc tttctctacc cattcctcca actacgattg cattactaat
    18721 tccgtcatca ggaattatag cataaccaaa accgtttcct ttattctcat gtcttgcttt
    18781 atgattttct aaggtttgaa cgtattgagt agaaagaaaa tattttgttg gaggaaccgt
    18841 ttcctcttta atatcagcaa aagttacttc tttatgcaaa ggtttcgggt atgtgaactc
    18901 atctattcct gtacttgtat gaaacccaac gatataaatt ctttctctat tttgaggaac
    18961 tccaaaatct ttagcattta taatttcagg ctcaggaaca aaataaccca aatcatttct
    19021 caaaacattt aagattgttg ctaatgtttt tcctccattg tggcttctta aacctttcac
    19081 attttctaaa aatatggctt ggggttgctt ccttttgata atttcggcaa catcaaaaaa
    19141 caaagttcct cttgtatctt caaaacctcc acgttttcct gcgatggaaa acgcttgaca
    19201 tgggaagcct gcacaaagca catcaaaacc atctggaata taagattttg tttcttcttt
    19261 ggtgatatct ccaaaaggag tttcaccaaa atttgcctta tatgtttttt ttgcatcttt
    19321 gtcccattca cttgtaaaaa cacattttcc tcctaaattt tgtaacgcca atcgaaaacc
    19381 tccaattcca gcaaataagt caataaattt gaattttggg ttttctaatg gagaaaaagg
    19441 tatctgaaaa gtgtcaaaca aaccatattg taatgcttct tcagcaactt gtgaagtaat
    19501 tttttcatct ggatatttgt actccaaaat ttcttttgta aatgccaatg caccttcttt
    19561 ataaaacttt gaaacaccat tttttgagtt gtgtaaatag tgagttaaaa cagcctcttg
    19621 gttgttttca ggttctgcca gcttaattct gaactgttta tctccaaata tttctaatga
    19681 tattttttct tttaaattca tttccaaaat tctttctctc actttcaatt agcaaaacgc
    19741 aagttaataa atttttctta tctgttttga ttcaacaaag ttagtcaata cgatttagtt
    19801 agattgtcat tctgtcaaaa attagaagtt acacagttat ttcaacactt gtatataaaa
    19861 agcacaatct ccctattcta taatactaaa tgctcaattt attgattatt gacaacattt
    19921 tatttttaat agtttatttt aagaacaaaa atttgactta atgtgtttta atttttaatt
    19981 taacacaaaa atatcgttaa gtaaaataaa tcaactatgt atccttatca aattaaaact
    20041 ttagaaaatt accataaagc ttacaaagat agtgtcgagc aacctgaaaa attttggttt
    20101 gaaattgcac aacattttaa atggaaaaag caacctacat tagaaaatac tttacattgg
    20161 aattttaaag aacctaaaat tgaatggttc agagatggaa ctttaaatat tactgaaaac
    20221 tgtttagata ggcatttaga aaaaaatgga aatacacctg ccttaatttt tgaacccaac
    20281 gcaccagaag ataaaaccat tacacttact tacaaagaat tacatcacaa ggtttgtcaa
    20341 tttgctcatg ttttaaaaaa taatggcgta aaaaaaggcg accgtgtttg tatttatatg
    20401 ggtatggttc ctgaattaag tattgcaatg ttggcatgtg ctagaatcgg tgcaattcat
    20461 agtattattt ttggtgggtt tagtgcaaat agcattagca gtcgattaga agatacacaa
    20521 gctgaatttg tcattacaca agacggctcc tatcgtggta acaaagaaat tgaattaaag
    20581 aaaatcatgg acgaagcttt agaaaatagt gctttagtga agaaagtaat tgttttccgt
    20641 cataccaaca acaattgtaa gatgaaagat ggaagagata tgctttggca agacgaaatt
    20701 accaaagtag aagctcaagg aaatcctgat tttcctgctg ttgagatgaa tgccgaagac
    20761 cctttgtata ttttatatac gagtgggagc actggaaaac caaaaggtgt tgtacatact
    20821 tgtggtggct atatggttta taccaactat acttttgtca atgtttttca atatcaacca
    20881 ggcgacatat ttttctgtac agctgatatt ggttgggtta cagggcacag ttatattgtg
    20941 tacggcccat tgagtgctgg agctacttcg ttaatgtttg aaggaattcc aactttccct
    21001 gatgctgggc gattctggga tattgtagat aaatataaag tcaatatttt atacactgca
    21061 cctacagcta tcagaagttt aatggcttat ggtgatagta tttttgatgg gaaagattta
    21121 agttcgttaa aagttttagg ttcagttggc gaaccgatta atgaagaagc ttggcattgg
    21181 tattacgata aagttggtaa aggaagatgt ccaatagtgg atacttggtg gcaaacagaa
    21241 aatggtggta ttttaatttc aggtttagct ggtattacac caatgaaagc agcacatgca
    21301 tcatttccat taccaggcat tcaacctatt ttgatggatg aaaaagcaac agtaattgaa
    21361 ggaagtgatg ccagtaaatc acaattaccc acagaaacta taagtggaaa tttatgcata
    21421 caatttccgt ggccaggtat gttacgaacc acatatagag accacgagcg ttgccgatta
    21481 aattattttt ctgcttatga tgataaatat tttactggcg atggctgttt aagagatact
    21541 gaaggaaatt acagaattac tggacgagta gatgatgtat taaatgttag tggacataga
    21601 attggaactg ctgaagtaga aaacgccatc aatgaacatc cactcatagt tgaaagtgcc
    21661 gtagttggtt acccacacga catcaaaggg caaggaattt atgcatatat cattttacaa
    21721 caaagtagtg aaaattttga tgcacaatct atcaaacaag aagtaaatca agtggttact
    21781 aagataattg gtgcgatagc taaacctgat aaaattcaaa ttgtgaaggg attacccaaa
    21841 accagaagtg gtaaaattat gcgaagaatt ttaagaaaaa ttgctgaagg cgataccagt
    21901 aatttaggtg atacatctgc attattagag ccagcaatcg tggacagtat caaagaaggt
    21961 aaagttcttt aacattggat atataattat tatttaattt gtaaaatcgt attttttatc
    22021 ttacttttag ataattgtaa aagatgaaaa acaagagaga aaattttatt aaaagtttgt
    22081 tggacaaatc tgacttacga gattatattc gtgtatttcc aaacaaagtt ggctcagaag
    22141 gttatgatgc ttggggtttt aatatcaaaa gtgtaaaggg tactattcgt ttagctaaaa
    22201 tattgtatga aaattatttt agagttgaag tatttggttt agaaaatata ccaaaagaag
    22261 gaagagtttt aattattcca aatcacagtg gacaattgcc aattgatggt gtaatggttg
    22321 gttatgcaat gctcactaat ccaaatggac caagagcacc aaaagctatg gcagaacgat
    22381 ttttcccaac attacctttt attgggaatt ggataaattc agtgggtggt gtcattggcg
    22441 atactgtaaa ctgtgaacga atgttggata atgatgaagc tattattgta tttccagaag
    22501 gcgttcgagg ctctggaaaa acctttgata aaagatatca attacaacga tttggtaatg
    22561 gttttatgca tttggcaatc aaacataaaa caccgataat tccagttggt atagtaggtt
    22621 tcgaagaaag tattatttca attgcagaca taaaagggtt agcaaaaatc ttggctgtac
    22681 cctatattcc attagctttg ccagccattt taccatcaaa agtatatata cattttggaa
    22741 aaccgatgta ttttgaagat ggagaattta ctgaagctga agtaaaagaa aaagtagatg
    22801 ctgtaaaaga agaaatcaag caattaataa aagaaggatt acaaaaacgt aaatcaattt
    22861 ttcaaaaata actatggaaa acagaaaaaa aataatgata acgggtgcag ccggtgcatt
    22921 agcaaaaaaa gtaatcgaac aattaaaaaa agattataaa attatcgctg tagattttag
    22981 aagtaaagtt gatttaggta ttccattaga gagctataaa gtcgatttta ataaacgaat
    23041 ctttgaagat atatttcgtg aacaccaatt tgaaggcatc atccatttag gaagaattgg
    23101 agcttccgag tccaatagac atgcacgata taatgccaat gtaattggaa ctagaaagct
    23161 ttttgatttg gcaaaaaaat atggtgtcaa acaaacaata gtgctttcta cttattttgt
    23221 ttatggagct tcgccttata atccatcttt gttagatgaa aaaacaccac ttaaagcatc
    23281 agaattaacc atggatttgg tagatagtgt agaattagaa aatctagcca atatttattt
    23341 atataaatat cctgaattaa atattactat tcttcgccct tgtaatattg ctggacctgg
    23401 tgttagaaat acattttctc agttactttc tgaaaaaaga gctccagtct tatggggttt
    23461 ttctcctttg atgcaattta tccatattga tgatatgcaa gaggcaattt gtaccgccta
    23521 taaacaaaac aaacctggtg tatataatat tgctccagca gattatattg catttcaaga
    23581 tgctgtaaaa gaggcaggtt gttcaacaat acaaatacct tctattcctc catcacttac
    23641 agacagaatc gcaagaatgt tgaattggaa aactttacca cctttcttaa ttaattattt
    23701 taaatatcca gttatcattg atggtagctt atttgaaaaa acattcaact ttaaacccaa
    23761 aagaactcta aaagaaattt tatcttatta taagaactta aagtagtttt taaagaaagg
    23821 cattatattt gtactatatt ttttaataaa ataatgacca agtgaaaaaa aattcatccc
    23881 tattagccat atttattttt atactattga ttgtggtttc ttgtgcagta aatcctgtaa
    23941 ctgggaaaaa acaactaagc tttatgtccg aagaacaaga gaaagcaatg ggcttagagt
    24001 ctgatcctgc tgtgatagca caatttggcg tatatccaaa tgatacatta caaaagttca
    24061 ttcaatctga aggtgatgca atggtaaaag tatctcacag acctgattta aaatttacat
    24121 ttagactttt agatgccaat gtcattaatg catttgctgt acctggtggt tatgtatatt
    24181 ttacacgaga aattatggct tattttaata atgaagctca atttgctggt gttttaggac
    24241 atgaaattgg ccatattacg gctcgtcatt ctgctcaaca atataccaaa caaatgatta
    24301 cacaagtagc attggtaggc ggaatgatat tctctgaaaa attgagaaat atgggtgaac
    24361 aagccatgca aggtatgcaa ttattatttc taaaattttc tagagatgat gaaacacaag
    24421 ctgataattt aggggtacaa tattcttcat tagtaggata tgatgccgtt gaaatggcaa
    24481 agttcttcaa aacactagaa agagtttc
//
LOCUS       DAOQCW010000024        23502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_796_length_23502_cov_5.614833, whole genome shotgun sequence.
ACCESSION   DAOQCW010000024 DAOQCW010000000
VERSION     DAOQCW010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 23502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23502
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_796_length_23502_cov_5.614833"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1143)
                     /gene="rmuC"
                     /locus_tag="PLS16_03590"
     CDS             complement(<1..1143)
                     /gene="rmuC"
                     /locus_tag="PLS16_03590"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNJ60195.1"
                     /translation="MEITSLIIGLLIGGVIAYIILYLLNKTKNVSKSEFDDLSAKHNE
                     TITNLRLYENKAQTLQETNDSLSNKITAKENEAVQLQTKATSLEEKLNNSESKINDLS
                     QRLTTELETNKSQQDEINLHKQKISELTANNTSITENLQKQSVTNDKQTKQIEELTAK
                     LTDLTSQVSKLTANNTALNDKLTSQKTEVEELQKTAHLQFEKIANKLFEEKSEKFTAT
                     NKTNIETLLNPLKEDINKFKEKVEATHTEETKQRTSLEERIKGLIEQTNKVSNEANNL
                     ATALKGKAQKRGNWGEMILERILESSGLTKDREYFVQQSIKDDEGKSQRLDVRVNLPD
                     SRVIIIDSKVSLIAYDNYSASEDSEEQKRFLAEHINATKLHIDQLSE"
     gene            1367..2458
                     /locus_tag="PLS16_03595"
     CDS             1367..2458
                     /locus_tag="PLS16_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNJ60196.1"
                     /translation="MNSENSKLFNPENYTFEFGIHRNKNIIWVVFPYNTDLLAKLKNT
                     VKAHWSQSNKKWYVADNLQHRILFGIEPNYFGKNAFLDISPINQPAFKRYIEQLKLKA
                     YSPNTIKTYSVEFAQLLKVLKNHAVDDLLPEKLRSYFLYCINTLKLSENLIHSRMNAV
                     KFYFEQVLHREKFFMEIPRPKKPSLLPKAISTQDIKKMLEILDNPKHQLLLKMCYGMG
                     LRVSELVNLKITDIDSKRMQVLVSSSKGKKDRYVVLPESVLEQLRTYYVEYKPKEYLF
                     EGQYGGKYSIRSVQQVFKSAMKKAKINKRVGVHSLRHSYATHLIEYGTDIRFVQELLG
                     HNNIKTTMIYTALTDASKRKIKSPLDFMD"
     gene            complement(2483..3136)
                     /locus_tag="PLS16_03600"
     CDS             complement(2483..3136)
                     /locus_tag="PLS16_03600"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HNJ60197.1"
                     /translation="MTKLIVLFSFLYSILGFQTTNTKDLNDANAKTLLDAVTKNYKSY
                     KSIQATFSIITEYPQSKTQTQNGTLFLKGEKYKILLGNQETYCDKTAVWTYLKDINEV
                     QINDYEPSKTEFSPANMFTIYQTDFNYMLNGKETLDGTTFSIVDLMPKDKTKPYFKIR
                     IWVDSKNNIKRLKIFDKNGTRYTYVVKTMNTSQTLEDSEFIFKASKYPGVHIEDLRL"
     gene            complement(3174..5807)
                     /locus_tag="PLS16_03605"
     CDS             complement(3174..5807)
                     /locus_tag="PLS16_03605"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNJ60198.1"
                     /translation="MPEPKNINTKEELPEILPDTTIISQNSIRLYLGIFCLFVAVFLS
                     VALFSYLFTWQEDQDKILDFNWSIIFNKSTVIENTMGTFGAIIAHLLVYELFGIAAFI
                     IPLFLFALGFNITLDEKIFNIKRLGLFSLIWIAFIATVSAFVLHQFSFPFGGSLGESI
                     NSWLSALIGKVGLGILLVIMGLSFFGINISFLLKSIRAKFQEIKQKRKELLQKEAEKA
                     KSIEENEAEVEKSLDKESGYDLESIIPEEKELPISHVKDNTFEILNEEKNEPTIEFIT
                     PNEVNPVSLIDISSETTDTKQDELEIISFEKEQPDDEIQLIVPEKEIKSNKLVAEKHL
                     DLEIDEVQPFGIIEPIEDEDEETPKVEIETNLENEEELIDENINYNPKLDLSKYQYPG
                     TNLLEDHGDGHIEINKEELEANKNQIVETLSYYNIGIEKIKATVGPTVTLYEIVPEKG
                     VRISKIKNLEDDIALSLSALGIRIIAPIPGRGTIGIEVPNVKKSIVSMKSLLESEKFK
                     KSDADLPIAIGRSISNEDFIIDLAKMPHMLMAGATGQGKSVGINAILISLLYKKHPSE
                     LKLVMVDPKKVELSLYKLIENHYLAKLPGEEEAIITDTKKVIHTLNALCIEMDDRYEL
                     LKAAGVRHIKEYNQKFINRRLNPQKGHRYLPYIVLVVDEFADLMMTAGKEVEAPIARL
                     AQLARAIGIHLVIATQRPSVNIITGTIKANFPGRIAFKVTSKVDSRTILDAGGADQLI
                     GRGDMLLSQGGDLIRLQCPFVDTPEVEKIAQYIGNQMGYPTPYELPEYVDPKESGGTE
                     KSFDSANKDELFEEAARLIVRNQIGSTSLIQRKLALGYARSGRLMDQLEAVGIVGPNL
                     GSKAREVYIKSEEELERILQD"
     gene            5988..8681
                     /locus_tag="PLS16_03610"
     CDS             5988..8681
                     /locus_tag="PLS16_03610"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNJ60199.1"
                     /translation="MKFKLFALMFVLGFFVSKTINAQSVVNEPSISLCESYSDYGEPK
                     GIASAWDIKPTGGFVYILYSNGEKKINSKKSLLLYVDKKNKAGKYEAFATETFKVESG
                     ANFSVYDYKFTEAGSYKIQAYQDGKILATTYATINVKSPTTTTNTTTKTNNQKSSGAS
                     IVACSEYDENGKPSGIYSIWNITEEAHIIPILFSTGGKAINTNNTLWLYVDKKDEKGN
                     YVPYGTEKFDVKAGNNWVVYNFSFDDPGEYKISAMQLSNELATTNITVKTKNETTLNK
                     SKKSIVTCTDFDTYGKPSGIYKEWNIQKSGSYVYVLFDNGGQKINGTLWLYVDKENES
                     GDYVAFDTKTFENTNNKTWANYKYTFTKPGNYKISAVSSKGEEAAVNVKVNFADNVVV
                     DVKKTIDLVDSYDDFGKPFGINTSWQIENNGKGGYVYVLFDNGEKMTGAYSLFVDKKN
                     TSGQYVEYDTKSFDNTGKNWSVFDYNFKESGEYKISAAKDNKEIVSTYATISYKKTDT
                     KPEAPKYQDNVTTAYYDGSEVLFGENATTSGTITGQNTIFTLASNNTKTVKMVFKQAK
                     AIKTNKIYVDIYSGNDYSNFVETKEITTTPTQQSFQLSYTFKQAGKYRFSIYNGDEIF
                     MNLGEVAINAPSTSNQTYTSQTNTNSNTTNNSNSIYNSLTFNNKVSKENRTALVIGNG
                     NYINAGKLPNPVHDAEEMAKVLKACGFNVTLIINANKKQMNEAVQQFGMDIANKKGTG
                     LVFYAGHGVQAKGENYLIPTDANLYGEEDLQYECVNVGQLLAKMDVAKNDMNILLLDA
                     CRNNPFERSWTRSQNGGGLASLNAPYGTIIGYSTNPGNVASDGTGYNSPYTTALLKFL
                     NKPSLTIEQVLKGVAYDVKNETPGQFPWYSSSITGEFYFRP"
     gene            8750..9538
                     /locus_tag="PLS16_03615"
     CDS             8750..9538
                     /locus_tag="PLS16_03615"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ60200.1"
                     /translation="MDILYRVEAYLTYLWIAKTKYQIHSPFLYKIITEVFEKTEIPER
                     FKKIEIVRNELLQNQNVIEVEDFGAGSKKFKSNNRKIADIAKISLKKQKYAQLLSQLI
                     VHFNSKNIIELGTSLGITTSYLALAHEKTKVTSIEGCKATADIAQQNFDKLNIKNVKL
                     VVGKFDDVLHSTLDKNETFDFVFIDGNHRELAVVEYFKTILPYCNNDTVIAIDDIHWS
                     EDMEEAWQFIKNHENVTVSIDIFEMGFVFIRKENIQKSHCVIQY"
     gene            9581..10696
                     /locus_tag="PLS16_03620"
     CDS             9581..10696
                     /locus_tag="PLS16_03620"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNJ60201.1"
                     /translation="MSENRSVVIYFSGLNGLRAIAALGVLFVHITQAMSHFGLNPYVF
                     GETNSNSIAIYRIALYSVSIFFGISGFLITYLLLKEKEKGSINIKYFYLRRVLRIWPL
                     YYLYLSLALITNWILGNPIDKGQLFFYLFLMANIPYVLGMSIPFLTHYWSLGVEEQFY
                     LFWPNWVKRIKHLLSSTLILILIFMSLKLFFYLFAKHPIYYKLYYILDANRFQCMLIG
                     AVFAILYYKNNEWFVNIFSSIYAQSIAWILFFMVFTNLFRISYFLGHELVTIATCTII
                     LAQIQQKNRIFNLENRVFNFFGKISYGIYVWHPLIILTLSKALIFKTKDSNLNYIIVY
                     LLSFCATILIAYLSYEYYEKQFLKLKSKFTTIKSRNS"
     gene            10749..11366
                     /locus_tag="PLS16_03625"
     CDS             10749..11366
                     /locus_tag="PLS16_03625"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNJ60202.1"
                     /translation="MLLEIFPFNIDERKIDKVVQVLKNGGLIIYPTDTVYGLGCDIYN
                     KQAVEKICKIKGIKPEKMNFSMICSDLSHISEYTVQFDNTIFRLMKRAFPGAYTFILK
                     AGHQIPKIFKNNKKTIGIRIPDHQIPLLIVEKLGNPILTTSIHHEDEVLDYITDPYAI
                     YERFENIVDIVIDGGAGGIQPSTIIDCSNGMPTITRKGAGSLDIL"
     gene            complement(11361..11942)
                     /locus_tag="PLS16_03630"
     CDS             complement(11361..11942)
                     /locus_tag="PLS16_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNJ60203.1"
                     /translation="MIKYNAIGIFILMLLGFNACQNSNAQSAGTGTPKSIYTYSAKDI
                     DGNTISMSNYKGKTIVIVNTASKCGLVDQLGEIEKFYQNYKDKNLVVLGFPSNNFLSQ
                     EPLSNAEIKTFCTKNYGVTFPMFEKIAVKGDDIAPIYKFLTSKTENGVLDAPVKWNYQ
                     KFIIDKNGFLKYSISPRTSVNDDEFKQALESVL"
     gene            12123..13550
                     /locus_tag="PLS16_03635"
     CDS             12123..13550
                     /locus_tag="PLS16_03635"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HNJ60204.1"
                     /translation="MDRSEFIKLLALAGVGTMIPTNLKALTLNEQEMTAAMFGNDFVW
                     GTATASHQIEGAWNVDGKGESVWDRFTHTTKKIRNRENADIACDFYNRYESDIELMRK
                     MNFNASRFSISWPRILPDGTGTVNQKGLDFYDRVIDKCLKENLDPWVTCYHWDLPQKL
                     QDKGGWENRDSLAWFENYVNIIGQKFGDRVKNWMVFNEPAVFTTLGYLLKIHAPGKFG
                     FKHFIPAVHHAALSHGIGGRVLKNVVKDGNIGTTFSFSHIDGWKEKASNLRAAKRMDA
                     LTNRLFIEPILGMGYPTDDLPKLKKIEKYMRAGDEQNLKFDFDFIGVQNYTRTVMKSL
                     CIVPAIHGINVSPKKLGHDITDMGWEVYPEGIYKVIKQVAAYPSVKKIYITENGAAFP
                     DVVENGAIHDEKRIKYYKDYLAQVLKAKQEGIDIGGYIAWSFMDNFEWAEGYKPRFGL
                     VHVDFETQQRTIKDSGKWFAEFLKK"
     gene            13657..15264
                     /locus_tag="PLS16_03640"
     CDS             13657..15264
                     /locus_tag="PLS16_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HNJ60205.1"
                     /translation="MQFDYVIVGGGSAGCLLANRLSENPVNTVCLLEAGPPDNSHFIH
                     NCNPVNMLFLMNSPKYNWRYYAEGNAKTGTRKFYWPRGKTLGGSSAVNAMIYTRGHTS
                     DYDHWASLGNKGWDWKSVLPYFKKSELQHREIDAFHSREGTMDVIDPTFHFPPSHAFV
                     KACVEAGIPYNHDINGEKYEGVDFFQINQTPEGRRCHSSVSFLDPVRSRKNLTVINYA
                     HIKRVLFKDKTAYALEYFDMKDGKQLKTIEANKEIILSAGIIGSPQILKLSGVGPKAE
                     LEKHDIPIVLDLPGVGENLQDHPDIIIRYKDKSSSSLVTVPKPYMVKFMSKFYRKNKE
                     PFIFTPTDAGGYVKSDSSVEVPDLQLQFASLRMLPHGAGLFTPSKPGFVLHVCHMRPN
                     SRGRVLLNSANPFDNPRIEANYFDDEMELNALVNGVKIARKIIAQKAFDAYRGEEEVP
                     GKSVCTDEQIRQFILDKAESVYHTAGSCKMGIDAMAVVDPELKIHGLKNIRVIDSSIM
                     PTITGSNIHAPTVMIAEKGADMILNGI"
     gene            15470..15868
                     /locus_tag="PLS16_03645"
     CDS             15470..15868
                     /locus_tag="PLS16_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60206.1"
                     /translation="MEFAQKVKEAVDTFVSESKTGIKELRYTAESTLKNVISEAQDEL
                     EVQKSNFKANADRIQAKLKGPFDLAKVQADVQEEVNILLGQVKNSYGRNAERVKNLVN
                     TAESKVENLAEEVKADVKKAAKATKEKVSK"
     gene            15996..16559
                     /gene="frr"
                     /locus_tag="PLS16_03650"
     CDS             15996..16559
                     /gene="frr"
                     /locus_tag="PLS16_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNJ60207.1"
                     /translation="MNEEVQMVLEETQSSMFKALKFMEEDLRKIRAGKASPDMLIGIT
                     VEYYGMPTPLNQVASVKLLDARTIVIQPFERKLIGDIERAIFQSNMGITPQNDGENIR
                     LQIPPMTEERRRDLVKQSKQEGEQCKIVIRNARKDANETIKDLQRDGLSEDEAKDAEA
                     SVQQLTNDHIAKVEKMLKIKEEEILTI"
     gene            16642..16950
                     /gene="rplU"
                     /locus_tag="PLS16_03655"
     CDS             16642..16950
                     /gene="rplU"
                     /locus_tag="PLS16_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNJ60208.1"
                     /translation="MFALVEIGGHQFKVSSNDEIFVYKLDGKEGDKITFDKVLLVSDA
                     TGTKVGTPKVDGVVVSATIVEQLKDDKVIVFKKKRRKGFKVKNGFRQSLTKIKIDSIA
                     "
     gene            16964..17212
                     /gene="rpmA"
                     /locus_tag="PLS16_03660"
     CDS             16964..17212
                     /gene="rpmA"
                     /locus_tag="PLS16_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNJ60209.1"
                     /translation="MAHKKGVGSSRNGRESESKRLGIKVYGGQAVIAGNIIVRQRGTK
                     FHPGDGVGLGKDHTIFALRDGSVVFKKGLKDRTYVSIA"
     gene            17346..19274
                     /locus_tag="PLS16_03665"
     CDS             17346..19274
                     /locus_tag="PLS16_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNJ60210.1"
                     /translation="MKRQIYYGFFIAFLFLQACSSTAKIKNGKMAFQYKNYEKAVEML
                     PQDFQKAKTNAAKIEIANNIFKSYQYLQNSDKMLEWSDALIKLGADNALDLKLLALKT
                     DGQYANALQFLQDYLLKNKDKSNLYKTELQTLSNIVANQDTTIKIINLKSLNSSASDY
                     GFIISDGKVYFTSNRNGKNKDVFTSNAFSQIYTADLVSDSTFTNVQPFTISDFPYHFA
                     QITFNKDHTEAYFAQCGSDDKIKNDYCKIYLSRFFNAAWTTPKQLTFFSDSIDVAQPF
                     LSPDDNELYFTSNDISGYGGSDIYVSKRDLNGNFVSASNLGHKINSVANETFPTYLDS
                     ILYFSSNRKGGLGGLDIYKTTKNGKFFENVQLMPLGINSGADDFALFYQDSSKIYVSS
                     NRKGTQGKDDIFLVVQTPKEIITKKKEPVYVLQILVKENIYKESGNPNSAIVGTQALE
                     NAETFIPFPIANKILYSDGNGKIEQIIYAPADFKVNVLKEGYLKKELLIDLEQIPVGD
                     LDTIFVQKEVILQKIFKNVEIVLENIYYDYDKANIREDAKKSLDALITILKDNPMIKI
                     ELAAHTDCRGADAYNLKLSQQRAESVVNYLIEKGVVSNRLVAKGYGETQLLEKCICTN
                     CTEEQHQKNRRTTFKIIE"
     gene            complement(19282..20028)
                     /locus_tag="PLS16_03670"
     CDS             complement(19282..20028)
                     /locus_tag="PLS16_03670"
                     /inference="COORDINATES: protein motif:HMM:NF040120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HNJ60211.1"
                     /translation="MNLFKLLLPNSDNLHIWITTAMMIIGLIFFNWKPEVIIFAYLFE
                     TVIIGVIHIIKMISVAIFSEKEKASPDKKSPWFVIPFFCFHFFFFIAVQMIFVFLFLD
                     IENLELSKGPFGLFHNFITLFSMPDMQEAYLIIILNNIFHTIKTFFVNGKYLESTLNE
                     LFMQPYLRIFVQQFVAILGGFLFMFTGAPKLIALLLIGIKTLVDLIGVSLPKNPALAE
                     YIINFLFKTKGKELTQKEIDDINNFRKTLE"
     gene            complement(20171..21427)
                     /gene="ilvA"
                     /locus_tag="PLS16_03675"
     CDS             complement(20171..21427)
                     /gene="ilvA"
                     /locus_tag="PLS16_03675"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HNJ60212.1"
                     /translation="MQKTTPTPEGIEQASLVLQNIIDKTPLQLNTNLSEEFDCNVYLK
                     REDLQQVRSYKIRGAYNFISRLSKAETKKGVVCASAGNHAQGFALSCFLLKIKGVVFM
                     PKTTPKQKVRSVQRIGKEFIEIELAGDSYDDAKYAALKYAQKEDSIFVPPFDHKLIIE
                     GQGTVALEILTQLGKKKLDYIFVPIGGGGLASGVITYFKKHSPNTKIIGVEPLGAPAL
                     AESIKNKKLTLLDKIDNFVDGAAVKQVGQLTFEIIKDNLDELLLIPEGRICTRILNLY
                     NRDAIVTEPAGTLSIAALDFTKYNLKGKNVACVISGGNNDIERMPEIKERSMIYEGLK
                     HYFMIKFPQRSGSLKQFVNKVLGPDDDITLFEYTKKNTKESGPALVGIELKNKEDYDG
                     LIDRMTKYNIDYTPINNNPILFNFLV"
     gene            complement(21459..22520)
                     /locus_tag="PLS16_03680"
     CDS             complement(21459..22520)
                     /locus_tag="PLS16_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60213.1"
                     /translation="MKKRIMLILKLLLFGGVIFGGYIILSILYALVTDYKPKTETKAE
                     VLNASSSSILHKDTLTFYTWNIGYCGLGAESDFFYDGGKMVRSSRELVNKNFSGVKET
                     ILDWKHDADFILLQEVDVDSRRSWGTNQLEGIKNLLDFNTVFGKNYQVRYIPIPLFNP
                     MGGVVGGIATYFPYQMSTEPIRYQFPANFSFPKGLFFLDRCFTKQTFKIDNGKNLVII
                     NTHNSAYDGGKLKKQEMAFFKNYIQQEYEAGNYVVVGGDWNQIPPGYTPKDKNSDYEE
                     IEIPNQYLPSNWTWAYDNTTPTNRKVDAPFVRNETYTTIIDFFLLSPNIKLTSVHGIS
                     DDFSYSDHQPVKMTCTLIK"
     gene            22574..23404
                     /locus_tag="PLS16_03685"
     CDS             22574..23404
                     /locus_tag="PLS16_03685"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HNJ60214.1"
                     /translation="MNISELNWKTNDGIDIYGRLWNTTNQPDFVLCIVHGMGEHIGRY
                     EHVAQFFTDNNIAVFGYDQRGHGKSGGPRGHYKSFDDFLDDVTLFLNQVDKKFPGVKK
                     IIYGHSMGGNVVSNYLIRRNQNFAGAILSSPYLELAFTPPAIKITLGKLMKNILPALS
                     LPSGLDASAISRDKSVVDAYLKDKLVHDKVSALMGIEMMETGKEAIERAAEIKIPILI
                     YHGTLDRLTSHDASQRFAQRCGSNARFISYEGLYHETHNEPEKELVLNNVLQFIKQLG
                     "
BASE COUNT         8162 a         3278 c         3880 g         8182 t
ORIGIN      
        1 ttcacttaat tggtcaatat gaagtttagt agcattgata tgctctgcta agaacctttt
       61 ttgctcttca ctgtcttcac ttgcagaata attgtcgtaa gcaattagag aaacttttga
      121 atcaataatg attactctgc tgtcaggaag attcactctt acatcaagtc tttgagattt
      181 gccttcgtca tctttgattg attgttgaac aaaatattct cggtcttttg tcaaacctga
      241 tgattcaagt atcctttcca aaatcatctc gccccaattt cctctttttt gtgctttacc
      301 ttttaaagct gtcgccaagt tatttgcttc gttgcttact ttgttggttt gttctattaa
      361 accttttatt ctttcttcta atgacgttct ttgcttggtt tcttccgtat gtgttgcttc
      421 aactttttct ttgaatttat ttatgtcttc ttttaacggg tttagaagcg tttcaatgtt
      481 tgttttgtta gttgcagtaa atttttcaga tttttcctca aacaatttgt ttgcaatttt
      541 ttcaaactgc aaatgagccg ttttttgaag ttcttcaact tctgtttttt gacttgtaag
      601 tttatcgttc aaagctgtgt tattcgctgt cagtttgctg acttgcgatg tcaagtcggt
      661 aagttttgct gttagctctt ctatttgctt tgtttgtttg tcgttcgtta cactttgctt
      721 ttgtaggttt tcagtgattg aagtgttgtt cgctgtcaat tcggaaattt tctgcttgtg
      781 tagatttatt tcgtcctgtt gacttttatt tgtctcaagt tcggtagtta gtctttgtga
      841 aaggtcattg attttacttt cgctattgtt tagtttttct tctaatgatg tcgcttttgt
      901 ttgtagctga acagcttcgt tttcttttgc agttatttta tttgaaagac tgtcatttgt
      961 ttcttgaagt gtttgggctt tattttcata cagtctaaga tttgtaattg tttcgttatg
     1021 ttttgcggac aagtcgtcaa actctgactt tgaaacattt tttgtcttgt taagcaaata
     1081 caagatgatg taagcaatta caccaccaat taaaagtccg attattaaac ttgttatttc
     1141 cattttaagt cgtctgtttt atttgttaca tttttcaggg tcgtcttttt acaatgaccg
     1201 ctaactcaca aatatacgca agtttttcaa attcacaaaa ataatattga aaatttgttt
     1261 ctcttttgta ttcaactatt ttagacaaaa atattgcttc gttttttgta ttgttttgtt
     1321 attttatgtt tatttgtatt tttatacaat acaaaatacg aagtatatga actccgaaaa
     1381 ttctaaactt tttaacccag aaaattatac tttcgaattc gggatacaca gaaacaaaaa
     1441 tataatttgg gtggtgtttc cgtacaatac agacttgctt gcaaaactca aaaatacagt
     1501 aaaagcacat tggagccaat ctaataaaaa atggtatgtt gcagacaatt tgcaacaccg
     1561 cattctcttt ggcatagaac ccaattattt tggcaaaaat gcttttttag atatttcgcc
     1621 catcaatcaa cctgcattca aaaggtatat agagcaactg aaattgaaag cttatagccc
     1681 taataccatc aaaacctatt cagtagaatt tgcacaatta ttgaaagttc ttaaaaatca
     1741 tgctgtagat gatttgttac ctgaaaaatt aagatcctat tttctctatt gtatcaatac
     1801 gctgaaactt tcagaaaatc tgattcatag cagaatgaat gccgtaaaat tttatttcga
     1861 acaagtattg catcgagaaa aattttttat ggaaattcct cgtcccaaaa aaccaagcct
     1921 tttacccaaa gctatttcta cacaagatat aaaaaaaatg ctggaaatat tagacaatcc
     1981 caaacaccaa ctactactga aaatgtgtta tggaatggga ctgcgtgtaa gcgaattggt
     2041 caatctgaaa attacggata tagatagcaa aaggatgcag gttttagtaa gttcgagtaa
     2101 aggtaaaaaa gatcgttatg ttgttttgcc agaatcggtt ttagagcaac ttcgaacgta
     2161 ttatgtagaa tacaaaccaa aagaatattt atttgaaggg caatatggtg gaaaatattc
     2221 cattcgttcg gtgcaacaag tttttaaaag tgcaatgaaa aaggcaaaaa ttaataaaag
     2281 agttggcgta catagtttaa ggcacagtta cgctactcat cttatagaat atggcacaga
     2341 tattcgtttt gtgcaggaac ttttggggca taacaatatt aaaaccacta tgatttacac
     2401 agcactgaca gatgcttcaa aacggaaaat aaaaagccct ttggatttta tggattaaga
     2461 agattgatag agaaaagtta ttttataatc tcaaatcttc gatatgaaca cctggatatt
     2521 tactagcttt aaaaataaat tcactatctt ctaatgtttg tgaggtattc atcgttttta
     2581 caacataagt gtaacgtgtt ccatttttat caaaaatttt caaacgtttg atattatttt
     2641 tgctatccac ccaaattcta attttaaaat aaggtttggt tttgtcttta ggcattaaat
     2701 ctacgataga aaatgtagtg ccatctaaag tttctttgcc attcagcata tagttaaaat
     2761 ctgtttggta aattgtaaac atattggcag gcgaaaactc agttttgctt ggttcataat
     2821 cattaatttg aacttcatta atgtctttta gataagtcca aacggctgtt ttatcacaat
     2881 aagtttcttg gttgcctaat aaaattttat acttttctcc ttttaaaaat aaggtgccat
     2941 tttgtgtttg agttttactt tgtgggtatt ctgtaataat ggaaaatgtg gcttgaatag
     3001 atttatagga tttatagttt tttgtaacgg catctaataa ggttttcgca tttgcatcgt
     3061 tcaaatcttt ggtattggta gtttggaaac ccaatattga atacaggaat gaaaataaga
     3121 caattaattt ggtcataatc taatatgtaa aatgtatttt aagtgatata tgcttaatcc
     3181 tgcaaaattc gttccaattc ttcttcggac ttaatataaa cttctcgagc tttactacca
     3241 aggtttggtc caacgatacc cacagcttcc aattgatcca ttaaacgacc agaacgtgca
     3301 tagcctaaag ctaattttct ttgaattaat gaagttgaac cgatttgatt tctaacaatt
     3361 aaacgtgccg cttcttcaaa taattcgtct ttattagcgg agtcgaaaga tttttcagtt
     3421 ccaccacttt ctttaggatc tacatattcc ggtaattcat aaggcgttgg gtaacccatt
     3481 tgattgccga tatattgagc aattttttct acttctggag tatccacaaa aggacattgt
     3541 aaacgtatca aatcgccacc ttgtgataaa agcatatcgc cacgtccgat gagttgatca
     3601 gcaccgccag catctaaaat cgttctagaa tctacttttg aggttacttt aaatgcaatt
     3661 cttcctggga aattggcttt aattgttcca gttatgatat tcacagatgg acgttgtgtt
     3721 gcgattacca aatgaatccc gatagctctt gctaattgtg ctaaacgtgc aattggagct
     3781 tctacttctt tgcctgctgt catcatcaaa tcggcaaatt catcaaccac caaaacaata
     3841 taaggtaaat aacgatgacc tttttgagga tttaatcttc tattgataaa cttttgattg
     3901 tattccttaa tatgtctaac gcctgcagct ttcagtaatt catagcgatc gtccatttcg
     3961 atacacaagg catttaaggt atgaattacc tttttggtgt cagtgatgat agcttcttct
     4021 tcacctggta attttgctaa atagtggttt tcaatcaatt tgtagagtga taattctact
     4081 ttttttggat cgaccattac caatttgagt tcagaaggat gtttcttata aagtaatgaa
     4141 ataagtatag cattgatacc aacagattta ccttgtccag tagcacctgc catcaacatg
     4201 tgcggcattt ttgctaaatc aataataaaa tcttcgtttg aaattgatct tccaattgca
     4261 attggcaaat ctgcatctga ttttttgaat ttctcacttt ctaaaagtga tttcattgat
     4321 acgatacttt ttttgacatt cggtacttca ataccaattg ttcctcttcc tggaattggt
     4381 gcgataatac gaatacctaa tgctgataaa ctcaaagcaa tgtcatcttc taagtttttg
     4441 atttttgata ttcttacacc tttttcaggc acgatttcat aaagtgttac agttggtcca
     4501 acagtagctt ttattttctc aatgccaata ttataataag aaagtgtttc tacaatttga
     4561 tttttgttgg cttctaactc ttccttattg atttcgatat gaccatcgcc gtgatcttct
     4621 aataaattcg ttcctggata ttggtatttt gataagtcta atttcggatt gtaattaatg
     4681 ttttcatcaa tcaattcttc ttcattttcc aaattagttt ctatttcaac tttaggagtt
     4741 tcttcatctt catcttcaat tggttcaata attccgaatg gctgtacttc gtcaatttct
     4801 aaatccaaat gtttttcagc tacaagttta tttgacttga tttctttttc aggtactatt
     4861 aattgaattt catcgtcagg ttgttctttt tcaaaagaaa taatttctag ttcatcttgt
     4921 tttgtatccg ttgtttcgct cgaaatatca attaagctaa caggatttac ttcatttggt
     4981 gtgatgaatt caattgtagg ctcattcttt tcttcattaa gaatctcaaa tgtattgtct
     5041 ttaacatgag aaataggtag ctctttttct tcaggaataa tactttctaa atcatagcca
     5101 ctttctttgt ctaaagattt ttctacttct gcttcgtttt cttcaataga ttttgctttt
     5161 tcagcttcct tttgtaatag ttcttttctt ttttgtttaa tttcttgaaa tttggctctg
     5221 atacttttta ataagaaact aatattgata ccaaagaaag acaaacccat aatcactaat
     5281 aaaattccca atccaacttt tccaatcaat gctgatagcc aagaattgat actttcgccc
     5341 aaacttccac caaaaggaaa tgaaaattga tgcaatacga aagcactaac tgttgctata
     5401 aatgcaatcc atattaaaga aaatagacct aatcttttaa tattgaaaat tttctcatcg
     5461 agggtaatgt tgaaacccaa agcaaataaa aaaagtggaa taatgaatgc cgcaatccca
     5521 aatagttcat aaactaataa atgggcaata attgcaccga aagtacccat tgtgttttca
     5581 ataactgtac ttttattaaa aatgatactc caattgaaat ctaagatttt gtcttgatct
     5641 tcttgccaag taaaaagata agagaataaa gcaactgata aaaatactgc cacaaataga
     5701 cagaaaattc ctaaatataa ccttatgcta ttttgtgaaa tgattgttgt gtcaggcaat
     5761 atttcaggta attcttcttt agtatttata ttctttggtt caggcattgt agcaaatgta
     5821 aaatgaatgt cttaaaaatt ataattaaaa atattcaaaa gtgtgaataa gttttttgaa
     5881 atcttaaaat tctttaaaag ataagactat tagatggaat ttatggtata ttatttgcaa
     5941 aaaatatata aaaagtataa attagcatca actataaaaa atctattatg aaattcaaat
     6001 tatttgctct aatgtttgta ttgggatttt ttgtttctaa aacaataaat gctcaatctg
     6061 ttgttaatga accatctatt tctttatgtg aatcttattc tgattatggc gaacccaaag
     6121 gcattgcaag tgcttgggat ataaagccta ctggtggttt tgtgtatata ttatattcca
     6181 atggagaaaa aaaaataaat tcaaaaaaat cattgttgct ttatgtagat aagaaaaata
     6241 aggctggaaa gtatgaagca tttgcaactg aaacttttaa agttgaatct ggtgctaatt
     6301 tttctgttta cgattataaa tttacggaag caggaagtta taaaattcaa gcttatcaag
     6361 atgggaaaat cttagcaact acttatgcaa ccattaatgt aaaaagccca accacaacaa
     6421 ctaatacaac cacaaaaact aataatcaaa aaagtagtgg agcatcaata gttgcttgtt
     6481 cagaatatga tgaaaatggt aaaccaagtg gtatttatag tatttggaat ataacagaag
     6541 aagcacatat tattcctatt ttattttcga caggtggcaa agcaatcaat accaataata
     6601 ctttgtggtt gtatgtggac aaaaaagacg agaaaggtaa ttatgttcct tatggtactg
     6661 aaaaatttga cgttaaggct ggaaacaact gggtggttta taatttttct tttgatgatc
     6721 caggagagta taagatatct gcaatgcaat tgagtaatga attagcaaca acaaatatta
     6781 ctgtaaaaac aaaaaatgaa acaacactaa ataaatctaa aaaatcaatt gttacttgta
     6841 cagattttga tacgtatggt aagccttcag gaatttataa agagtggaat attcaaaaaa
     6901 gcggaagcta tgtgtatgta ttgtttgata atggaggaca gaaaataaat ggaacacttt
     6961 ggttgtatgt agataaggaa aatgaaagtg gtgattatgt agcctttgat accaaaacat
     7021 ttgagaatac caataataaa acttgggcaa attataaata tacctttaca aaacctggta
     7081 attataaaat ttcagcagtg agttcaaaag gcgaagaagc tgctgtaaat gtaaaggtaa
     7141 attttgcgga taatgttgtc gttgatgtta aaaaaacgat tgatttagta gattcttatg
     7201 atgactttgg aaaacctttt ggtatcaata cttcttggca gattgaaaac aatggaaaag
     7261 gcggttatgt ttatgtactt ttcgataatg gagagaaaat gacaggtgct tattctttat
     7321 ttgtagataa aaaaaatact tctggtcaat atgtagaata tgatactaaa tcatttgata
     7381 ataccggaaa gaattggagt gtttttgatt ataattttaa agaatcagga gaatataaaa
     7441 ttagtgctgc taaagataat aaggaaattg tatctactta tgcaacaatt tcttataaaa
     7501 aaactgacac aaaacctgaa gcaccaaaat atcaagataa tgtaaccacg gcttattacg
     7561 atggttcgga agttttattt ggagaaaatg cgacaacaag tggcacgata acagggcaaa
     7621 ataccatatt cacactagct tctaataata ccaaaacagt gaaaatggta tttaaacaag
     7681 ccaaagctat taaaaccaat aaaatttatg tagatattta tagcggaaat gattacagta
     7741 attttgtaga aacaaaagaa attaccacga caccaacaca acaaagtttc caattatctt
     7801 atacttttaa acaagcagga aaatatcgct tttcaatcta taatggagac gaaattttta
     7861 tgaatttagg agaagttgct atcaatgcac caagtacttc taatcaaact tatacttctc
     7921 agaccaatac gaattctaat acgaccaata attctaattc tatttataat agcttgactt
     7981 ttaataataa agtttctaaa gaaaatagaa cagctttagt aattggaaat ggcaattata
     8041 taaatgctgg aaaattacca aatccagtgc atgatgctga agagatggca aaagttttaa
     8101 aagcatgtgg ctttaatgtt actttaatta tcaatgccaa taaaaagcaa atgaatgaag
     8161 ccgttcaaca atttggaatg gatattgcca ataaaaaagg aacaggttta gtattttatg
     8221 ctgggcatgg tgtgcaagct aaaggcgaaa attatttaat tcctacagat gcaaatttat
     8281 atggagaaga agatttgcaa tacgaatgtg taaatgttgg acagttatta gcaaaaatgg
     8341 atgtagccaa aaatgatatg aatattttat tgttagatgc atgtcgaaat aatccttttg
     8401 agagaagctg gacacgttca caaaatggtg gaggtttggc aagtttgaat gcaccttatg
     8461 gaacaatcat aggttattct actaatccgg gcaatgtagc ttcagatggt acaggttata
     8521 attctcctta cacaacagcc ttgttgaaat ttttgaataa accgagtttg acaattgagc
     8581 aagtattgaa aggcgttgct tatgatgtga agaatgaaac accagggcag tttccttggt
     8641 atagttcatc cattacaggc gaattttatt tcagacctta aaataaaaag attaactttg
     8701 tgcgaaataa gtaaacattg ttacttattt cgtacaatat tataaattta tggacatatt
     8761 atacagagtc gaagcatatt taacctatct ttggatagca aaaactaaat atcaaattca
     8821 ttctcctttt ttatataaaa ttataacaga ggtatttgaa aaaacagaaa tacctgaaag
     8881 atttaaaaag attgaaattg taagaaatga attacttcaa aaccaaaatg ttattgaagt
     8941 agaagatttt ggagcaggtt ctaagaagtt taaatccaat aacagaaaaa ttgcagatat
     9001 tgctaaaatt agtttgaaaa aacaaaaata tgctcagtta ctcagtcaat tgattgttca
     9061 ttttaattct aaaaatatta tcgaattagg aacttctctt ggaattacga cttcatattt
     9121 ggctttggct catgagaaaa caaaagttac ctcaatagaa ggttgtaaag caactgctga
     9181 tattgctcaa caaaattttg ataaattaaa tatcaaaaat gtaaagttag tcgttggaaa
     9241 atttgatgat gtgttacaca gcacactaga taaaaatgaa acttttgact ttgtttttat
     9301 agatggtaat catcgtgaat tagcagtggt tgaatatttt aaaactattt tgccctattg
     9361 taataacgat accgtgattg ctatagatga tattcattgg agcgaagata tggaagaagc
     9421 ttggcagttt attaaaaatc atgaaaacgt tactgtatct attgatattt ttgaaatggg
     9481 ttttgtattc atcagaaaag aaaacattca aaaatctcat tgcgtgattc aatattaggt
     9541 agtttaaata tcgtatcttg ttacattaaa taacttatct atgtctgaaa atcgctcagt
     9601 ggtaatttat ttttctggac taaatggatt aagagcgatt gcagctttgg gtgtactttt
     9661 tgtacatatt actcaagcaa tgtcccattt tggattaaat ccttatgttt ttggtgaaac
     9721 taactcgaat tctattgcta tttatcgaat agccttgtat tcagtaagta ttttctttgg
     9781 aataagtgga tttttaataa cctatttgct tttaaaagaa aaagaaaaag gcagcatcaa
     9841 tataaaatac ttttatttaa gaagagtatt gcgaatttgg ccactttact atctttattt
     9901 gtctttagca ctcattacga attggatttt aggaaatcca atagataaag gtcagttgtt
     9961 tttttatctt tttttgatgg ctaatattcc ttatgtatta ggtatgtcaa taccattttt
    10021 gacacattat tggtcattgg gagtggaaga acaattttat ttattttggc caaattgggt
    10081 aaaaagaatc aagcatttgc taagttctac cttgattttg atattgattt ttatgtctct
    10141 taaattgttt ttttatctct ttgctaagca tccaatttac tataaattat attacatttt
    10201 agatgctaat cgttttcaat gtatgctaat tggtgctgta tttgctatct tatattataa
    10261 gaataatgaa tggtttgtaa atatattttc tagcatttat gctcaatcta ttgcatggat
    10321 tttgtttttt atggtattca ctaatttgtt taggatttct tattttttag ggcatgaatt
    10381 agttactatt gcaacttgta ccataatttt agctcaaatt caacaaaaaa atagaatatt
    10441 caatctagaa aatagagttt ttaatttctt tggaaaaata tcttatggga tttatgtttg
    10501 gcatccactt ataattttaa cactttctaa ggcattgatt tttaaaacaa aagattcaaa
    10561 tcttaattat attatagttt atttgctttc tttttgtgca actatcttga ttgcttactt
    10621 atcgtatgaa tattatgaaa agcaattttt gaagcttaaa tcaaaattca caacgataaa
    10681 aagtaggaat tcataaagat tattcctaat tttaagaatc gcttttaaat ctaattaaag
    10741 acctaatcat gttattagaa atttttccgt ttaatataga cgaaaggaaa atagataaag
    10801 ttgtacaagt actgaaaaat ggcggattaa ttatttatcc gacagataca gtttatggtt
    10861 taggttgcga tatttataac aaacaagcag tagaaaaaat ttgtaaaatt aaaggcatta
    10921 aacctgaaaa aatgaatttt tctatgatat gttcagattt aagtcatatt tctgaatata
    10981 ctgtacaatt tgacaatact attttcagat taatgaaacg tgcatttccg ggtgcttata
    11041 cttttatact aaaagccggt catcaaattc ctaaaatatt taaaaataat aaaaagacga
    11101 taggtattag aattccagac catcaaattc ctttgcttat tgttgagaaa ctaggtaatc
    11161 ctattttgac aacttctatt caccatgaag atgaagtatt ggattatata acggatcctt
    11221 atgcaattta tgaacgcttt gaaaatattg tagatattgt aatcgatggc ggagctggcg
    11281 gcatacaacc ttcaacaatt attgattgtt cgaatggaat gccgacaatt actcgtaaag
    11341 gagcaggaag tttggatatt ttataataca ctttctaatg cctgcttaaa ttcatcatca
    11401 ttgacggagg ttctcggact aattgaatac tttaaaaaac catttttatc aataataaat
    11461 ttttgataat tccattttac tggagcatct aaaacaccat tttctgtttt ggaagtcaag
    11521 aatttataaa taggtgcaat atcatcacct tttacagcaa ttttttcaaa cataggaaaa
    11581 gtaacaccat aattcttagt acagaaagtt tttatttcag catttgataa tggttcctga
    11641 gataaaaaat tgtttgaagg aaaacctaat acaaccaagt ttttatcttt ataattttga
    11701 taaaattttt ctatttctcc caattggtca accaagccac atttacttgc agtatttact
    11761 atcacaattg ttttgccttt ataatttgac attgaaattg tattgccgtc aatatctttt
    11821 gcactatatg tataaataga ttttggtgtt ccggtaccag cactttgagc attgctattt
    11881 tgacaagcat tgaatcctaa aagcattaaa atgaatatgc ctatagcgtt gtattttatc
    11941 atgacatttg attttctatt caaagttagt caatttttag actaaatgtt atactttttg
    12001 ataggataat tttgttgtaa gatttagagc attgaaaata atatttttgt tcttattgag
    12061 aatatttttt tgtatcttac aacactaaca aaaaaacaaa aaacactaaa ttttttcaaa
    12121 gaatggatag gagtgaattt atcaaattat tagcattggc aggagttgga acgatgatac
    12181 caacaaattt aaaagcacta acacttaatg aacaagaaat gacagcagca atgtttggaa
    12241 atgattttgt atggggaaca gcaacagctt ctcatcaaat tgagggagct tggaatgtag
    12301 atggaaaagg agaatcagtt tgggatagat tcacacatac aactaaaaaa attagaaata
    12361 gagaaaatgc tgatatagct tgtgattttt ataatcgcta tgaaagtgat attgagttaa
    12421 tgcgaaaaat gaactttaat gcttctcgtt tttcaattag ctggcctaga attttgccag
    12481 atggaacagg aactgtaaat caaaaaggat tagattttta cgaccgagta attgataaat
    12541 gtttgaaaga aaatttagac ccttgggtaa cttgttatca ttgggatttg cctcagaaac
    12601 tacaagataa aggtggttgg gaaaatcgag atagtttagc ttggtttgaa aattatgtaa
    12661 atataatagg tcaaaagttt ggcgatagag ttaagaattg gatggttttt aatgagcctg
    12721 cagtttttac tacattaggt tatttattaa aaattcatgc accaggaaaa tttggattta
    12781 aacattttat tccagctgtt catcatgctg ctttaagtca tggtattggt ggtcgtgtgc
    12841 tgaaaaatgt tgtaaaagac ggaaatattg gaactacttt ttcattttca cacattgacg
    12901 gttggaagga aaaagcatca aatttaagag ctgcaaaacg aatggatgca ttgacgaacc
    12961 gtttgtttat agagcctatt ttaggaatgg gttatcctac tgatgactta cctaaattga
    13021 agaagataga aaaatatatg cgagcaggtg atgagcaaaa tttaaaattt gattttgatt
    13081 ttatcggagt tcaaaattat acaagaacag tcatgaaatc gttgtgtatt gttcctgcaa
    13141 ttcatggaat taacgttagt cctaaaaaac taggacacga tattacagat atgggttggg
    13201 aagtttatcc tgaaggcatt tataaagtaa ttaagcaagt tgcagcatat ccaagtgtta
    13261 agaaaattta tattacagag aatggtgcgg catttcctga tgtggtagaa aatggtgcaa
    13321 ttcatgatga aaaaagaatt aaatattata aagattattt ggcacaagtg cttaaagcca
    13381 aacaagaggg aattgatatt ggaggttata tagcttggtc atttatggat aattttgaat
    13441 gggcagaagg ttataaacca cgatttggtt tggttcatgt tgattttgaa acacaacaac
    13501 gaactattaa agattcaggt aaatggtttg ctgagttttt aaaaaaataa atagtatata
    13561 attagttttt ttcttttttt agacataaaa attttatgta ataaaggaat agttattttt
    13621 aattttctat ctttatttaa gtaaaaaaat gtattgatgc aatttgatta tgttatagta
    13681 ggtggaggtt ccgcaggttg tcttttagca aatcgtttat ctgaaaatcc tgtaaatact
    13741 gtttgtctat tagaagctgg acctcctgat aattctcatt ttattcataa ttgcaatcct
    13801 gtaaatatgt tatttctgat gaatagtccg aaatacaatt ggcgttatta tgcagaaggt
    13861 aatgccaaaa caggtacacg taaattttat tggccacgag ggaaaacttt gggaggctca
    13921 agtgctgtga atgcaatgat ttatacacgt ggacacactt ctgattatga ccattgggct
    13981 agtttaggaa acaagggttg ggattggaaa tctgtgttgc cttattttaa aaagtctgaa
    14041 ttacaacatc gtgaaatcga tgcttttcac agtcgtgaag gtactatgga tgttatagac
    14101 cccacttttc attttccgcc aagtcatgct tttgtgaaag cttgtgttga agcagggatt
    14161 ccgtacaatc acgatattaa tggagaaaaa tatgaaggtg tagatttttt tcaaatcaat
    14221 caaactccag aaggtcgaag atgccattct tcagtatcat ttttagaccc agttcgcagt
    14281 agaaaaaacc ttacagtcat taattatgct catatcaagc gtgttttatt taaagataaa
    14341 actgcctatg cattggagta ttttgatatg aaagatggaa agcaactaaa aactattgaa
    14401 gcaaataagg aaattatttt gagtgcaggt attattgggt caccacaaat tttaaaactg
    14461 tcaggtgttg gacctaaagc agagttggaa aagcatgata ttcctattgt tttagatttg
    14521 cctggcgtgg gagagaactt acaagatcat cctgatatta ttatcagata taaagataaa
    14581 tcaagctcat ctttagttac agttcccaaa ccttatatgg taaaatttat gtctaaattt
    14641 tatcgtaaaa ataaagaacc ttttattttt acacctacag atgcaggtgg ttatgtaaaa
    14701 tcggattcaa gtgttgaagt tccagacttg caattacaat ttgcttcact cagaatgttg
    14761 ccacacggtg ctggattatt tacgccgtca aaacctggtt ttgtcttaca tgtttgccac
    14821 atgcgtccaa atagtcgtgg tcgggtttta ttaaattcag ccaatccatt tgataatcca
    14881 cgtatagaag ctaattattt tgacgatgaa atggaattaa atgctttagt gaatggtgta
    14941 aaaattgcaa gaaaaattat tgcccaaaaa gcatttgatg cttatcgtgg agaggaagaa
    15001 gtgccaggga aaagtgtatg tacagatgag caaattcgtc agtttatttt agacaaagcc
    15061 gagtcagtgt atcacacagc aggttcttgt aaaatgggta ttgatgctat ggcagtagtt
    15121 gatccagaat tgaaaattca tgggcttaaa aatatacgtg ttattgattc atctattatg
    15181 ccaacgatta caggaagtaa tattcatgca ccaactgtaa tgattgcaga aaagggagct
    15241 gatatgattt taaatggaat ttagtttgtc tttttttgaa taagatagat atctaatgat
    15301 aaataagtag ctgtaaattt attgcagtta agctttaaca ttggtataac ctctacgata
    15361 tgtaaaatac atttttatat caatatttga taattatcaa aatttgacaa gtataaacac
    15421 ttgatgtaat tttgcattac attttataac aattaaaatc tttttaaaaa tggaatttgc
    15481 acaaaaagta aaagaagcag tagatacatt tgtatctgaa agcaaaactg gtatcaaaga
    15541 attaagatac acagcagaat caacattgaa aaatgttatt agcgaagctc aagatgaatt
    15601 agaagttcaa aaaagcaact tcaaagctaa tgcagacaga atccaagcta aattaaaagg
    15661 tcctttcgat ttagcaaaag ttcaagctga tgttcaagaa gaagtaaaca ttttattagg
    15721 tcaagtaaaa aattcttacg gtagaaatgc tgaaagagtt aagaacttag taaatacagc
    15781 tgaatctaaa gttgaaaact tagctgaaga agtaaaagct gatgttaaga aagctgcaaa
    15841 agcaacaaaa gaaaaagtat ctaagtaatt agaacagtta gcttactatc atttttaaaa
    15901 agagccaccc aaagttatag aggtggctct tttttattta ataaaacttt agaataaacc
    15961 aaagtaattc gtatttttgc actagaaaat aagttatgaa cgaagaagta caaatggttt
    16021 tagaggaaac tcaatcctct atgtttaagg cactgaaatt tatggaagaa gatttacgta
    16081 aaattcgtgc tggtaaagca agtccagata tgttgattgg tatcactgtt gaatattatg
    16141 gaatgccaac accattgaac caggtagcaa gtgtgaaatt attagatgca agaaccatag
    16201 taattcaacc atttgaaaga aaattaattg gtgatattga aagagcaatt tttcaaagca
    16261 atatgggtat aacaccacaa aatgatggcg aaaatatccg tttacaaatt ccaccaatga
    16321 cagaggaacg tcgccgtgat ttggtaaaac aatccaaaca agaaggtgag caatgtaaaa
    16381 ttgtgattag gaatgcacga aaagatgcca atgaaacgat taaagatttg caaagagatg
    16441 gactttctga agatgaagca aaagatgcag aagcaagtgt acaacaatta accaatgacc
    16501 atattgcaaa agttgaaaag atgttgaaaa tcaaagaaga agagatttta acaatatagt
    16561 attttatcaa aatataaaat ttgaataaaa gatacttttt aattaaaaat aatgtatctt
    16621 tgcaactcga aaaataatat tatgtttgca ttagtagaaa taggaggaca ccaatttaaa
    16681 gtttcatcaa acgatgagat ttttgtgtac aaattagacg gaaaagaagg cgataaaata
    16741 acttttgata aagtattatt ggtttctgat gctaccggaa caaaagttgg tacaccaaaa
    16801 gttgatggtg ttgttgtgtc tgcaacaatt gtagagcaat taaaagacga taaagtaatc
    16861 gtttttaaga aaaaaagaag aaaaggcttt aaagtaaaaa atggttttag acaatctttg
    16921 acaaaaatta aaattgattc aatagcttaa aaaatataaa gaaatggcac acaagaaagg
    16981 cgttggtagt tcaagaaacg gtagagagtc agaaagtaaa agactcggta ttaaagttta
    17041 tggtggtcaa gctgtaattg ctggcaatat catcgtaaga caaagaggaa ctaaatttca
    17101 tcctggagat ggtgtaggtt taggaaaaga ccacactatt tttgcattga gagatggatc
    17161 agtagttttc aaaaaaggtc ttaaagatag aacatatgta agtattgctt aattgcaatt
    17221 gtaaatttta tagaagtgcg ttcattgaac gcactttttt tattttgcca ttttcctttt
    17281 aaaaaagagg gtaattactt tattaattgc ttttggtctt gtatctttgg aattgtatgt
    17341 gtgttatgaa aagacaaatt tattacggat tttttattgc ttttttattt ttacaagctt
    17401 gttcatcgac tgcaaaaatt aagaatggaa aaatggcatt tcaatataaa aattatgaga
    17461 aagcagtaga aatgttaccg caagattttc aaaaagcaaa aactaatgct gcaaaaattg
    17521 aaattgcaaa taatattttt aaaagctatc aatatttaca gaatagtgat aaaatgttgg
    17581 aatggagcga tgcactcatt aaacttggtg ctgataatgc tttggattta aaattgttag
    17641 ctttaaaaac ggatggtcaa tatgctaatg cactccaatt tttacaagat tatttgctga
    17701 aaaataaaga taaaagtaat ctctataaaa ctgaacttca aacactttct aatattgttg
    17761 caaatcaaga tacaacaatc aaaataatta atttaaaaag tttaaattca tctgcatctg
    17821 attatggttt tattatttcc gatggaaaag tttattttac atcaaacaga aatggaaaaa
    17881 ataaagatgt atttacttca aatgctttct cacagattta cactgcagat ttagtttctg
    17941 attctacctt tacaaatgtt caacctttta ctataagtga ttttccttat cattttgctc
    18001 aaattacttt taataaagac catacagaag cttattttgc acaatgtggc tcagatgata
    18061 aaattaaaaa tgattattgt aaaatctatc tttctagatt ttttaatgca gcttggacaa
    18121 caccaaaaca attgactttc ttttctgatt ctattgatgt cgcacagcct tttttatcgc
    18181 ctgatgataa tgaattgtat tttacatcaa atgacattag tggatatggc ggaagtgata
    18241 tttatgtttc taaacgtgat ttgaatggca attttgtttc tgctagcaat ctaggacata
    18301 aaattaattc tgttgcaaat gaaacgtttc cgacttattt agatagtatt ttgtactttt
    18361 catcgaatag gaaaggagga ttgggtggat tggatattta taaaactaca aaaaatggaa
    18421 agttttttga aaatgtacaa ttaatgcctt tgggtattaa ttctggtgcc gatgattttg
    18481 ctttgtttta tcaagatagc tctaaaattt atgtttcttc taatagaaaa ggtacacaag
    18541 gaaaagacga tatcttttta gtcgttcaaa caccaaaaga aataattact aaaaagaaag
    18601 aaccagttta tgttttgcaa attttagtaa aagagaatat ctataaagaa tcaggaaatc
    18661 ctaattctgc tattgttgga acacaagctt tagaaaatgc agaaacgttt attccctttc
    18721 caattgccaa taaaatattg tattctgatg gcaatggtaa aattgaacaa ataatatatg
    18781 caccagcaga tttcaaagta aatgttttga aagaaggcta tttgaaaaaa gaacttttaa
    18841 ttgatttaga acaaattcca gttggagatt tagataccat atttgtacaa aaagaagtga
    18901 ttttgcagaa aatatttaag aatgttgaaa ttgttttaga gaatatttat tacgattacg
    18961 acaaagccaa tatacgtgaa gatgcaaaga aaagtttaga tgctttaatt actattttga
    19021 aagataatcc aatgattaaa attgaattag ccgctcatac cgattgtaga ggtgctgatg
    19081 catataactt aaaactttcg caacaaagag cggaaagtgt tgtaaattat ttgatagaaa
    19141 aaggagttgt ttcaaataga ttagtggcaa aaggttatgg agaaacacaa cttttagaaa
    19201 aatgtatttg cacaaattgt actgaagaac aacatcaaaa aaatagacga acaactttta
    19261 agataattga ataaaatggt tttattcaag tgttttcctg aaattattaa tgtcatcgat
    19321 ttctttttga gtaagttctt tgccttttgt tttaaataga aaattgataa tatattcagc
    19381 caaagcagga ttctttggta aagagacacc tattaaatct actagtgtct taattccaat
    19441 tagtaaaagt gcaatgagct ttggagcacc agtaaacata aataaaaatc cacctaaaat
    19501 agcaacaaat tgttgaacaa atattcttaa atatggttgc ataaataatt cattgagcgt
    19561 actttctaaa tattttccat ttacaaagaa tgttttaatg gtatgaaaga tattgtttaa
    19621 aattattatt aggtaggctt cttgcatatc aggcatcgaa aataaagtaa taaaattatg
    19681 gaataaccca aatggtcctt tacttagttc taagttttct atatctaaaa ataagaatac
    19741 gaaaatcatt tgaacagcta tgaagaagaa gaaatgaaag cagaaaaatg gaataacaaa
    19801 ccaaggcgat tttttatctg gagatgcttt ttccttctct gaaaaaattg caactgaaat
    19861 catttttata atatgaatca cgccaattat tacggtttca aataaatatg caaagatgat
    19921 aacctcaggc ttccaattaa aaaatattaa tccaataatc atcatagcgg tggttatcca
    19981 tatatgtaga ttgtcagaat ttggaagcag taacttaaaa agattcatag aaatagtttt
    20041 ttctaaatgg aagataaata attttgtgcg gaaataataa aatacttgaa agttaaactt
    20101 agttttttat ttcaaatttt taaattagaa ataaataatt cagtgcatta taaatcaaag
    20161 tatagaaaaa ctatactaag aaattgaata aaatgggatt gttatttatt ggtgtataat
    20221 caatattgta tttggtcatt ctatcaatta aaccatcata atcttcctta ttttttagtt
    20281 caataccaac caaagctgga ccactttctt ttgtgttttt cttagtgtat tcaaataaag
    20341 taatatcatc atctggacct aaaaccttat tgacaaattg ctttaaagaa ccagaacgtt
    20401 gaggaaactt tatcataaaa tagtgcttca atccttcata tatcattgaa cgttccttga
    20461 tttcaggcat acgttctata tcattattgc caccgcttat tacacaagcg acatttttac
    20521 cctttaaatt atattttgta aaatctaaag ctgcaatgga taaagtacca gctggctcag
    20581 taacgatagc gtctctattg tacaaattta aaattcgtgt acaaattctt ccttcaggaa
    20641 taagcaataa ctcatctaaa ttatctttga taatttcaaa agtcagttgc cctacttgct
    20701 tgactgctgc accatcaacg aaattatcta ttttatctaa taatgtcagt tttttgtttt
    20761 tgatggattc tgctaaagct ggtgcaccta atggctctac gccaattatt tttgtattag
    20821 gactgtgttt tttgaaatac gttattacac cagatgctaa tcctccgcca ccaattggaa
    20881 cgaatatata atctaatttt ttctttccta attgtgttaa gatttcaaga gccacagttc
    20941 cttgcccttc aataattaat ttatgatcaa atggtggaac aaaaattgaa tcttcctttt
    21001 gagcatattt taaagctgca tatttagcat catcgtaaga atctcctgct aattcaattt
    21061 caataaattc tttacctatt ctttgtacag aacgtacttt ttgtttgggc gtagttttgg
    21121 gcataaaaac aacacctttt atttttaata aaaaacaaga caaagcaaat ccttgtgcat
    21181 gatttcctgc actagcacaa acaacgcctt tcttagtttc tgctttgctc aatctagaaa
    21241 tgaaattgta ggcaccacga attttataag agcgaacttg ttgtaagtct tcccttttta
    21301 gatatacatt acaatcaaac tcttctgaaa gattcgtatt taattgtaat ggagttttgt
    21361 cgataatatt ttgtaaaaca aggctagctt gttctattcc ttctggcgtt ggagttgtct
    21421 tttgcataat tgaaatgcaa aactaataaa atgtctgctt attttatcaa agtacaagtc
    21481 atttttacag gttgatggtc agaataagag aaatcatctg aaattccatg tacacttgtc
    21541 aattttatat ttggcgatag aaggaagaaa tctataattg ttgtataagt ttcatttcga
    21601 acaaaaggag catctacttt tctattggta ggtgttgtat tgtcataagc ccaagtccaa
    21661 tttgaaggta gatattggtt aggaatttca atttcttcgt aatcgctatt tttatcttta
    21721 ggtgtataac ctggaggaat ttgattccaa tctccaccaa caacaacata gttgccagct
    21781 tcatattctt gttgaatata gtttttgaaa aatgccattt cttgcttttt tagtttgcca
    21841 ccatcataag cagaattatg cgtattaatg attactaaat tttttccatt atctatttta
    21901 aaagtttgct tcgtaaaaca tctatctaag aaaaacaagc cttttggaaa tgaaaaattt
    21961 gcaggaaatt ggtatcggat aggttctgta ctcatctgat aaggaaagta agtagcaatt
    22021 ccaccaacga caccacccat tggattaaat aatggaatag gtatataacg tacttggtaa
    22081 tttttcccaa aaacagtatt gaaatccaat aaatttttga tgccttctag ttgattcgtt
    22141 ccccaacttc ttcttgaatc tacatctact tcttgtaata aaataaaatc agcatcatgc
    22201 ttccaatcaa gaatagtttc tttaacgcca gagaaatttt tattcactaa ttctctacta
    22261 gaacgaacca tttttccacc atcataaaag aaatcagatt ctgcacctaa tccacaataa
    22321 ccaatattcc aagtataaaa agtaagtgta tccttatgta aaatagatga actacttgcg
    22381 ttgagtacct ctgcttttgt ttcggtttta ggtttataat cagtaactaa tgcatataat
    22441 atggatagga taatatatcc tccaaatata actcctccaa ataataaaag ttttaagatg
    22501 agcataattc tttttttcat cagcaaaaat tattagtttt atgctttaaa attaacaata
    22561 tttttttaag tttatgaata tttcagaatt aaattggaaa actaacgacg gaattgatat
    22621 ttatggtcgt ttatggaata ctacaaatca acctgatttt gtattatgta tcgtacatgg
    22681 tatgggcgaa catattggaa gatatgaaca tgtagctcag ttttttacag ataataatat
    22741 tgcagtgttt ggttatgacc aacgtggaca tggtaaatct ggagggccaa gaggtcatta
    22801 taaaagtttt gatgatttct tagacgatgt tacgttattt ttgaatcagg tggataaaaa
    22861 atttccgggg gtaaagaaaa tcatttatgg acatagtatg ggtgggaatg ttgtatcgaa
    22921 ttatttaatt agaagaaatc aaaactttgc tggtgcaatt ctatcatcgc cttatttaga
    22981 attggcattt acgccacctg ctattaaaat tactttgggt aaattaatga aaaatatttt
    23041 gccagcattg agtttgccaa gtggtttaga tgcatctgca atttctagag ataaatcggt
    23101 tgttgatgct tatttgaaag ataaattagt tcacgataaa gtgtctgcat taatgggaat
    23161 tgagatgatg gaaacaggaa aagaagctat tgaaagagca gcagaaatta aaattccaat
    23221 tttgatttat catggaactt tagatagatt aacttcgcac gatgcaagtc agcgatttgc
    23281 tcaaagatgt ggcagtaatg caagatttat aagttatgag ggtttgtatc atgaaacaca
    23341 taatgaacct gaaaaagaat tggtattaaa taatgtactc cagtttataa aacaattagg
    23401 ataatgattt atgcctaatc ggaatttttt agagaaatta ttgcagaaat taaaaggcaa
    23461 tgatgctcgc tctattcatc taaatgcttt accttctaat tt
//
LOCUS       DAOQCW010000025        23400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_799_length_23400_cov_5.024759, whole genome shotgun sequence.
ACCESSION   DAOQCW010000025 DAOQCW010000000
VERSION     DAOQCW010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 23400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23400
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_799_length_23400_cov_5.024759"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PLS16_03690"
     CDS             complement(<1..637)
                     /locus_tag="PLS16_03690"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60215.1"
                     /translation="MNKGNDSGILLKAIYLIAVILIVRLFFLQVLSPQYKLRAKDNVL
                     KKITIYPSRGLILDRHQNVMVYNEAVYDVLIQLNLTKNIDTALLASLLNSNIDFIRTK
                     LAEIKVTTPNKPTPLFKLVDQITFSKFQEHLFQFPSISIQTRTVRHYPYNAGAAIFGY
                     LSEVNPKDIENSGGYYEMGDYFGKTGLESYYDKYLRGEKGVTYALVDVKNTY"
     gene            complement(644..1159)
                     /locus_tag="PLS16_03695"
     CDS             complement(644..1159)
                     /locus_tag="PLS16_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60216.1"
                     /translation="MNKEFIYINTIRTVFFLILQIFLFNILPIGIFYIMVYPAAILLF
                     PIATRKVLIVIIAFSCGFILDWFSNGNGLHTASLTFLGYSRSYILEYIRPSSGWTKID
                     VPNIYQQGFKWVATYTTIAIIIHHSVYFFLESFSAINVLFTLKKMVLSSIMSIAIILL
                     INLLFLKNNKL"
     gene            complement(1152..1979)
                     /gene="mreC"
                     /locus_tag="PLS16_03700"
     CDS             complement(1152..1979)
                     /gene="mreC"
                     /locus_tag="PLS16_03700"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNJ60217.1"
                     /translation="MRNIIFLITKFHTLIVFVVLEIFALSFVFKTNNYQGIKYANTSN
                     FIAANVVNLSNEIINFINSSKNNKILTEENAKLRAQLTYQNNYSEDTLLPKKSDTYTY
                     SYIPAKVINNTINNNNNFITINKGTLDGIKNGYGVVSSKGVVGIIVNTTEHYALIMSA
                     ISTKSNISVRHKNTNAIGSLYWNGNNPFELNIDNFSKTLPIKIKDTIVTAGFSSIFPP
                     NLPVATVKKISPNPTSSFYICEVILTNSIPSLTDVYVVVNKNIEEIKKLNEPITINE"
     gene            complement(1993..3018)
                     /locus_tag="PLS16_03705"
     CDS             complement(1993..3018)
                     /locus_tag="PLS16_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNJ60218.1"
                     /translation="MGLFSFLNRDLAIDLGTANTIVIYKDQVVVDEPSIVAIDKKTEE
                     VIAVGKKAQLMHEKTHANIETIRPLKDGVIAHFKAAEAMIRGFIKMADDGKRSFFTPS
                     LNLVICIPSGITEVEKRAVFESAEHANARERYLIHEPMAAALGIGLDVELPEGHMIID
                     IGGGTTDIAVIALSDIVTDQSIRVAGDDFNRDIVEYVRRQHNTLIGERSAEQVKIHVG
                     SALQDLENPPEDFAIHGRDLLTGIPKQITVSYTEIAGALDKSISKIEEAIMKALESTP
                     PELASDIYKKGLYLTGGGALIRGLDKRLMLKTKLPVRIGDDPLRAVARGTGIALKNRH
                     RFTFLMS"
     gene            complement(3137..4654)
                     /gene="purH"
                     /locus_tag="PLS16_03710"
     CDS             complement(3137..4654)
                     /gene="purH"
                     /locus_tag="PLS16_03710"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNJ60219.1"
                     /translation="MKAIKNVLVSVYYKDGLDELIHILAKQAVNFYSTGGTQKFIESL
                     GYKVNTVEGLTAFPEILGGRVKTLHPKIFGGILARRDNEQDQQQLVQFEIPEIDMVIV
                     DLYPFEETVASTTDEQTIIEKIDIGGISLIRAAAKNFKDTLIVSNKSQYATIANLLAE
                     NKGATALETRKLFAKEAFAVSSHYDTLIYRYFAENKDENIFKQSYNDGEALRYGENPH
                     QKGFYFGNLEEVFDILNGKALSYNNLVDIDAAVRLMTEFTEPTFAVLKHTNSCGVASR
                     NSILEAWQAALAGDPVSAFGGVLIANRTIDEATALEIDKLFFEVLIAPEFDEKALDIF
                     TKKKNRILLKQKLFSFPNIQYKSLLNGAIVQDFDTKIEVADELKSVTKLTPSSSEIED
                     LLFANKIAKHLKSNTIVLAKNKQLLGMGCGQTSRVDALQQAIHKAQSFGFDLKDSVMA
                     SDAFFPFPDCVEIANNEGIKTVIQPGGSIKDKLSIYYCDDNEMKMVTTGIRHFKH"
     gene            complement(4880..6142)
                     /locus_tag="PLS16_03715"
     CDS             complement(4880..6142)
                     /locus_tag="PLS16_03715"
                     /inference="COORDINATES: protein motif:HMM:NF022019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNJ60220.1"
                     /translation="MNKLTLINPYIHQEAKLKKDKFLDRIKNVQQIRACYLERNFLPG
                     EDIEWEILEELHKAINTGMFSFGISNYKIFAFEINKELYDWFKNNDLKTIQKQVINWI
                     AAAYLSRPDMQEYLDEYIYSPILSEVKNVICKHYTNDFAKKYNLQKQVFKLFELASDG
                     QKDKVKSNTHESSQEEYFEENDKLYSEKEKEYNMASLSYINCLPPKFLMNPLKESNAL
                     LFDDFGLKNSPFIRIRIAYFTKSDWIFIIERDLQKAKELFKHELDRGHLTIDEIKRIL
                     KDAESDTLSRSESDREIWNNWLYSYKKFKKNNNHIFRLFEEDTTVGLIYIVRQRNTNY
                     FKIGWTERKNNSTDKQAVEIRISSLQTGNPEPIDIVGFFKASGTKTERTLHRIFESKK
                     RTGEWFLLTDSDWKNILNDDWRISNKIF"
     gene            complement(6688..7137)
                     /locus_tag="PLS16_03720"
     CDS             complement(6688..7137)
                     /locus_tag="PLS16_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60221.1"
                     /translation="MKHLFIYLSLLITTICYGQHVSMNETLGIYEYSHIKEFKDGINT
                     RLDLFAQKMKESNYSDVNITDKGIKGENFFTKMILGSAMEVHFNAFIDFKEGKYKLTI
                     NNFRIKDVRYGTVALETIGKNSQKKWVNFINEKLPQVVANLENTDKW"
     gene            complement(7454..8890)
                     /gene="dnaA"
                     /locus_tag="PLS16_03725"
     CDS             complement(7454..8890)
                     /gene="dnaA"
                     /locus_tag="PLS16_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNJ60222.1"
                     /translation="MKSKHELVWESCLKIIKDNISLQSFTTWFEPIKPIKVENFVLTI
                     QVPSQFFYEWLEEHYVTLLRKTIKRELGDQAKLEYRVLMESKNAANTNPKTMDLPNNR
                     EGIYGANEVSLPTMLSTPMVKNPFVIPGLKKIHVDSQLNPNYLFETFIEGDCNKFARN
                     AAKAVAKSPGGNAFNPLVIHGGTGLGKTHLAQAIGNFIKTNTPGKTVLYVGSEKFTTQ
                     FLNALREQSTNDFIHFYQQIDVLIIDDIHYLGNKAKTQDIFFHIFNHLHQNNKQLILT
                     SDKAPKDLEGVEDRLLSRFKWGLTTDLQSPDYETRVSILRKKMHADGVEMSEDVVRFI
                     ATNIKNNVREIEGVVTSILAQATLNKQEINLDLAKRTVLSFVKHATKEISLEMIQKTV
                     CDYFEVPVEKLKEPTRKRQFVQARQLSMYFAKEFTKASLKSIGQQFGGRDHSTVIHSC
                     QAVKNLVDTDEDFKDSVDELRKRIELSL"
     gene            complement(8978..9886)
                     /locus_tag="PLS16_03730"
     CDS             complement(8978..9886)
                     /locus_tag="PLS16_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNJ60223.1"
                     /translation="MQNRRNFLKTAITTTAALALHQPIFSSNKSKQLTILHTNDVHSR
                     IEPFPATDLKYPGMGGAAMRSTLINKIRTENEHTLLLDAGDIFQGTPYFNFFKGNVEI
                     DVMNELGYECSTLGNHEFDNGIDALAKQIDRANFPFVNCNYDFTNTLLNGKIHKHITL
                     QKGSLKIGITGCGVELKNLVAPYNYEGIVYHDPIDKIQEQVDILKHKKKCDIVIVLSH
                     IGYAYNTNKISDLQLAQKTKDIDIIIGGHTHTFLEEPTIIQNLENKDVLINQVGFAGI
                     FLGQIDYIFEKKSTKKQAKNHIVIPT"
     gene            complement(9876..10613)
                     /locus_tag="PLS16_03735"
     CDS             complement(9876..10613)
                     /locus_tag="PLS16_03735"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HNJ60224.1"
                     /translation="MLRTYFKYGLIFLILLTVSCKHYQVSTKHYDNIQINNIALDSSI
                     YNLYKPYKDSLDKIMLLPIAILSENVIKQQPSSTLGNLIADILFDETQKLSKDTVDFA
                     ITNYGGIRVNTLQKGTLKILDAFNIMPFENTIGVLSINQEEVQMLCDVIAQKGGWPIS
                     NQINFRIKDGKAIDILINQKPLQENKIYKVAIIDYLVSGGDNMSFLKDKPYINFNVLF
                     RDAIIEYLKNTKEPIIPSNQKRIMYAE"
     gene            complement(10594..11130)
                     /locus_tag="PLS16_03740"
     CDS             complement(10594..11130)
                     /locus_tag="PLS16_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60225.1"
                     /translation="MSFFLEILKYTLPSLIMLIGIYLIIVRFLEQNEKLKKLELKKLS
                     IQENSKIALPLRLQAYERVILFLERIHPNQLVQRVREANMTVAALNLELAKAIRIEYE
                     YNLSQQIYLNQDAWILVERAKDETLKIINSIASNLPKEANGIELSKAIFQYLAESNQE
                     FPTHIAINFIKEDVKNLF"
     gene            11259..11606
                     /locus_tag="PLS16_03745"
     CDS             11259..11606
                     /locus_tag="PLS16_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HNJ60226.1"
                     /translation="MENTSSLPINITNSALEEIKNLYQQIENKTEIGLRIGVEGGGCA
                     GFSYIMNFEKQKEGDNKYILEGLIPIYIHPTQEIYLSGTIIDFKTGLDNRGFVYNNPN
                     ATEICGCGTSFSA"
     gene            11751..12035
                     /locus_tag="PLS16_03750"
     CDS             11751..12035
                     /locus_tag="PLS16_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003272088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNJ60227.1"
                     /translation="MNKGDLVKVIADAAGVTNAKATDALNAILDGMGKTLKKGDKVTL
                     VGFGTFSTSKRAKRIGRNPSTGEKITIPAKTTVKFKAGKELSESVNKTKK"
     gene            12115..13362
                     /locus_tag="PLS16_03755"
     CDS             12115..13362
                     /locus_tag="PLS16_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNJ60228.1"
                     /translation="MALNYIWISFFVISFIVAIVKTILGDTEVFSLLTTKLFESTKIG
                     FEIALGLTALMAFWLGIMNIADKSGLIKKCSNLLAPFFSKLFPEVPKESPAYGNMMMN
                     FSANMLGLDNAATPLGLKAMNDLQNLNNQKTVASKAMIMFLVINTAGITLIPTSVMAI
                     RQTMAIEQGLVGFNAADIFLPTLMVTYCTLFFAIIAVAWHQRLNLFNLPFITFNLLFI
                     GIISGMYFWIKQFPQEAIAGKIALIGSVLIFGIIVVIIISGAIKKINVYDVFIDGAKD
                     GFKTAVQIIPYLIAILAAIAVFNATGCLNFITDGIGNIVRLLGFDDKFIPALPVGLMK
                     TLSGSGARAFMVDVMHNYGVNSFQGNLASIMQGSTETTFYVLAVYFGSVGIKNAKSAI
                     FFGLLADFVGIIIAIAVAYLFYS"
     gene            complement(13373..14482)
                     /locus_tag="PLS16_03760"
     CDS             complement(13373..14482)
                     /locus_tag="PLS16_03760"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNJ60229.1"
                     /translation="MRILIIFFISAISFISCTIAPGTVPAGLTFTSIFNTKNTTGISC
                     YRIPTITTAPNGDLVAAIDERVPDCGDLRTNKDINILIRRSSDNGKTWTAIEKVIDYP
                     NGQSASDPSFIVDKTTNTIFMFYNYMNLNTEKDIYYFMVTKSSDNGKTWSTPVDITSQ
                     VTKSSWHNHFKFITSGNGTQTKDGKLLHTIVNLNSGLYVFGSDNHGDSWYLIDKSITP
                     GDESKIIELNDGTWMINSRVNKVGYRYIHTSTNEGLSWTTISAPALPDPGCNASILRY
                     SSVAEGDDKNRLIFVNAKDAKQRKNLTIRISYDEGKTWSAGKTIYTGGAAYSSLTLLK
                     NGEIGLIFEKDEYKDNTFVSISMKWLTDGKDLGIQ"
     gene            complement(14626..16239)
                     /locus_tag="PLS16_03765"
     CDS             complement(14626..16239)
                     /locus_tag="PLS16_03765"
                     /inference="COORDINATES: protein motif:HMM:NF017050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HNJ60230.1"
                     /translation="MSNNDLTRRKFVGLSAMATIATMGGTTFSLAGCKKDKVENVPSS
                     EQDVVIIGSGFGGAISALRLAEHGVKALMLEMGRQYTVSKYEDVFAKSLQPDGRSSWL
                     KKKTNVPVVNDFLQFSIYKYVGVLDRIDYDTSYTRIYRGTCLGGGSVVYGAMLPYARP
                     ELWDKYFPYINYQDMTDKWYPKVKSVLDFSTVPDELLNSKMYDYARVALDHCDKAGMT
                     PIFLNAGVNFDVIKGELDGTEKKCCMNGDVIYGAYNGYKNSLDRNYIPAAIATGNLTV
                     QTMSKVDYINKRSDGRYEVFVDIIDEVGEKIDRKLYIAKKVFINAGVAGTMDILLKSK
                     YKGGLSNLSDEVGKHWGGNGNIMAMRTGLKENTGNVQGAVPVVAYGYLDSPVTPTLAE
                     QAPLPIGIELKQLLTLAITENPERGYFEYDPNTDRAELHFDKSQMDISRRGMQAFIDK
                     LNEANGGSLSNIYFNGNSFGDNFSYHPLGGACLGLASDYYGRLKGYEGLYCLDGSMMP
                     GFSCCANPALTIAAIAERNMENIIAEDFS"
     gene            complement(16350..17336)
                     /locus_tag="PLS16_03770"
     CDS             complement(16350..17336)
                     /locus_tag="PLS16_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNJ60231.1"
                     /translation="MQIYNNILETIGNTPIVKLNKITAHIPATVCVKVEYGNPGNSIK
                     DRMALKMVNDAENDGRLKPGGTIIECTSGNTGMGLAIVAAVRGYKCIFTTTDKQSKEK
                     FDMLRALGAEVIVCPTNVSADDERSYYSIAKRLATEIPNSIWMNQYDNLSNRQAHYES
                     TGPEIWKQTDGKITHFVVGIGTGGTVTGTSKYLKEQNPNIKTWGIDTYGSILKQYHET
                     GTFDPAEIHSYITEGIGEDIIPQNYDFSLIDFIEKVTDKEAALMQRRLAVEEGLFLGN
                     SAGSAVQGLLQLHKKFPLTKDDLVIIVTHDHGSRYVGKFFNDEWMREKGFIN"
     gene            complement(17408..17959)
                     /locus_tag="PLS16_03775"
     CDS             complement(17408..17959)
                     /locus_tag="PLS16_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60232.1"
                     /translation="MKYTTTQTLLLIFSSVILLQCTKQPLPINENELITRVQLTFTDT
                     LGESSSFLYCINDGIFGNKKDTISLMKNKTYSLEISFYNVQSASVVDSINNEILEEAE
                     EHQIFIQSITIDSFKYMDEDNNAYPIGLKTLIKTNNILSLEYFRIILIHQPNKKGLAV
                     AQNQVKNAGGATDVDISFLIKLH"
     gene            complement(17965..20265)
                     /locus_tag="PLS16_03780"
     CDS             complement(17965..20265)
                     /locus_tag="PLS16_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ60233.1"
                     /translation="MRFIISIIFLLFTNKLLASDCYYTLNGQIFNQYNTPLEFVEIKI
                     LENQTLIKSDEQGKFVIPNLCIQTYHFEFNHINCQTDTIIIHIKGDRKQTFYLEQLVY
                     FLDEFTKTDAKLSDNLQMTNTINKFSMQQLSGKDLAQAIQAINGIRVIKSGSNINKPM
                     FNGFYGDRITLVNNGVLFQSQDWGTDHAPEIEISNINKIEILNNSQTVEYSTQNIGAT
                     ITIDNPLISYKEAIKFGLSYGLQSNGWQNSLATYFQKSFKKQYAFRIQTAFKKSADLH
                     TPRYTLSNTAQQETSLSFQQKFNIKKSTIQTIYSLFSNENGILLAAHIGNIEDLKNAI
                     NSDTPIIIEPASYKIKNPKQFTQHHLLDIKLVQPLRKHHFIEYRTNFQNNRRQEYDIR
                     RGNRSSTPALNMHLISYYGKLVNHSEKYLKTNWKWHQKSGLIYQYKNNTNDPNTGIIP
                     IIPDYLQYNFAIFSIQEFFKNNISFDVGVRYENQQLNSYTFNKKELIIYKKNYNTYNF
                     TANFSYLQKYFDYQTSISLSSKAPNISELFSNGLHHGTATIEYGNKMLKIENSYALSN
                     AFNLKYRKYILLNIDYKLQYLKNFIYLQPNSDFLLTIKGAFPVFQYQSTNAFFNNIKI
                     HTQINITDYFQFQFQSNLIRAKNLKTKDYIVNIPADQLKFGLKFYGDTKVLKNAYLQI
                     NGDYILKQKRTPKLIKDYKDVPNAYFLLNLQLGTSVGKANKIMLSFAAENILNKNYRD
                     YLNRFRYFTDEIGFNFLFKINYFLNH"
     gene            20449..20922
                     /locus_tag="PLS16_03785"
     CDS             20449..20922
                     /locus_tag="PLS16_03785"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNJ60234.1"
                     /translation="MEDLFKEAADKMVIYNVRKSWLIIAKLYNEMTAKFGGTISMAFV
                     LLALYEEEGVPVTKIAPRIGMEPNSLSRILKELESKGILERRKNHSDSRIVNIVLTEQ
                     GKKLRKYALKSVFNLEKNVKEDISENDLIGFFNVLEKIPSAINKIQEKVNQQRNK"
     gene            20956..21642
                     /locus_tag="PLS16_03790"
     CDS             20956..21642
                     /locus_tag="PLS16_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ60235.1"
                     /translation="MKIIDIQNIKREFKLGDELIQALKGVDLQIQKNEYVALMGPSGS
                     GKSTLMNILGCLDTPSSGQYLLNNIDVSSMNDNDLATIRNKQIGFVFQTFNLIPRLST
                     LDNVALPLVYAGWNKSKREERAKEVLEQVGLAERMKHKPNELSGGQRQRVAVARALVN
                     FPSLILADEPTGNLDSKTSYEIMALFDEIQRLGNTVVLVTHEEDIAKYTHRIVRLRDG
                     LIESDLPNNR"
     gene            21693..22259
                     /locus_tag="PLS16_03795"
     CDS             21693..22259
                     /locus_tag="PLS16_03795"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNJ60236.1"
                     /translation="MKVYTKTGDLGDTALYGGQRVSKSDIKIDSYGNVDELNSSIGVV
                     VSLLNEQKLLPETVEQLHEIQEILFDIGAHLAKQLGKEKLALPEIKEDYINILEQQID
                     KWTEELPELKFFILPGGNIIAAHTHIARTVCRRAERSAVLLSHQESVEPILIKYLNRL
                     SDYLFVLSRYINYISKSVEIIWLGRLRK"
     gene            22410..>23400
                     /gene="obgE"
                     /locus_tag="PLS16_03800"
     CDS             22410..>23400
                     /gene="obgE"
                     /locus_tag="PLS16_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNJ60237.1"
                     /translation="MEGNNFIDYVKIFTKSGDGGPGSAHFRREKYVPKGGPDGGDGGR
                     GGHIILRGNKQLWTLLHLKFQKHVKAQNGEGGKGALRYGKQGDDIILEVPLGTVGKDA
                     ETGEKEVEIMEDGQEVIWLKGGKGGLGNNHFKTSTNQAPHYAQPGLPGVEGWKILELK
                     VLADVGLVGFPNAGKSTLLSVLSAAKPEIGDYAFTTLVPSVGVVSYRDNLSFVMADIP
                     GIIEGASKGKGLGLRFLRHIERNSVLLFVIPADCDDIKKELKILEKELKLYNPEIAYK
                     KKLLGISKSDYLDAELKSLIKKELPKKIKSVFFSAQSGDGLMELKDQLWQLMNE"
BASE COUNT         7779 a         3447 c         3479 g         8695 t
ORIGIN      
        1 tataggtgtt ttttacatca actaatgcgt aagttacacc tttttcgcct ctgagatatt
       61 tgtcataata actttctaat ccagtttttc caaaataatc acccatttca taatatccgc
      121 ctgagttttc tatatccttt ggattaactt cacttaggta tccaaaaatt gctgctccag
      181 cattgtaagg ataatggcgg acggtacgtg tttgtataga aatactagga aattggaata
      241 aatgttcttg aaatttagaa aatgtaattt gatctactaa tttaaaaagt ggagttggct
      301 tatttggcgt tgttacttta atttctgcta attttgtacg aataaaatca atattagaat
      361 ttagtaaaga tgctaataag gcagtatcga tatttttagt tagattcaat tggattaaaa
      421 catcataaac tgcttcatta taaaccatta cattttggtg tctgtctaaa attaaacctc
      481 ttgaaggata tatggtaatt ttttttaaga cattatcttt tgctctgagt ttatattgtg
      541 gacttaatac ttgcaaaaaa aacaaacgaa ctataagtat aactgcaatg agatatatag
      601 ctttgagtaa aatgccacta tcatttccct tgttcatttt aacctacaat ttattgttct
      661 ttaaaaataa taaattaata agtaagataa tagcaataga cataatggaa cttaatacca
      721 tcttttttaa agtgaataaa acatttatag ctgaaaatga ttctaaaaaa aagtaaacag
      781 aatggtggat aattatagct atggtagtat atgttgccac ccacttgaaa ccttgttggt
      841 aaatatttgg cacatcaatt ttagtccaac ctgaactcgg tctgatatac tctaaaatat
      901 aacttcttga atagcctaga aaagtaagac ttgctgtgtg taagccattt ccattactaa
      961 accaatctaa aataaaacca caactaaatg caataattac tattaatact tttcgagttg
     1021 caattggaaa taataatatg gctgcgggat aaaccataat gtaaaaaatg cctatcggta
     1081 gaatattaaa taggaaaatc tgtaatatca aaaagaaaac agtccttatt gtattgatat
     1141 agatgaattc cttattcatt gatagttatt ggttcgttta attttttaat ttcttctatg
     1201 tttttattga caactacata aacatcagtt aaactaggaa ttgaattggt taatattact
     1261 tcacaaatat aaaaacttga ggttgggttt ggtgatattt tttttactgt tgccactggt
     1321 aaatttggtg ggaaaataga ggaaaatcct gcggttacaa tggtgtcttt gattttaata
     1381 ggtaatgttt tactaaaatt atcaatattt aattcaaatg gattatttcc attccagtat
     1441 aaactaccaa tagcattcgt gtttttatgt cgaacactaa tattagattt tgtactgatg
     1501 gctgacataa ttaaagcata atgttctgta gtatttacta taataccaac cacacctttg
     1561 ctacttacta cgccatatcc atttttaatt ccgtccagag tgcctttatt tattgttata
     1621 aagttgttgt tgttattgat agtgttattg ataacttttg cagggatata agaataagta
     1681 taggtatccg atttttttgg caataaagta tcttctgaat aattattctg ataagttaat
     1741 tgagcgcgta attttgcatt ctcttcagtt agaattttat tattcttaga agaatttata
     1801 aaattgataa tttcattgct aagatttaca acatttgcag caataaaatt tgaagtatta
     1861 gcatacttaa taccttgata attgttggtt ttaaatacaa aagaaagagc aaatatttct
     1921 aatactacaa atactattag tgtatgaaat tttgtaataa gaaatattat attccgcatg
     1981 cataaacaat tattagctca tcaagaaagt aaagcgatgt ctatttttga gggcaattcc
     2041 tgtgcctctt gctactgcac gaagtggatc gtcgccaatt ctaactggta attttgtttt
     2101 taacattaat cttttgtcta aaccacggat taatgcaccg ccgcctgtta aatacaatcc
     2161 ttttttataa atatcagatg ctaattctgg tggcgtactt tccaaagcct tcataattgc
     2221 ttcttcaatt tttgaaattg atttatctaa tgcacctgca atttccgtat aggaaacagt
     2281 aatttgctta ggaatgcctg ttaataaatc tcgaccatgg atagcaaaat cttcaggtgg
     2341 gttttctaaa tcttgcaatg cagaaccaac gtgaattttt acttgttctg ctgaacgctc
     2401 tccaattaat gtattgtgtt gtcttctaac atattcaaca atatctctat taaaatcatc
     2461 tccagcaaca cgaatagatt gatcggttac aatatcagat aaagcaatta cagcaatatc
     2521 tgttgttccg ccacctatat caataatcat gtggccttca ggtaattcaa catctaaacc
     2581 aattcctaaa gctgctgcca ttggttcgtg tattaaatat ctttctctag catttgcatg
     2641 ttctgctgat tcaaatactg ctcttttttc tacctcagta attccagatg gaatacaaat
     2701 taccaaattt aatgaaggag taaaaaaact tcttttgcca tcatctgcca tctttatgaa
     2761 tccacgaatc attgcttcag ctgccttaaa gtgagcaatt acgccatctt ttaaaggtcg
     2821 tattgtttca atattggcgt gtgtcttttc gtgcattagc tgagcttttt taccaacagc
     2881 aattacctct tctgtttttt tgtctattgc aactattgaa ggctcatcta caacaacctg
     2941 atctttatat ataacaattg tatttgcagt acctaagtct atcgctaaat ctctatttaa
     3001 aaaactaaat aagcccattt tatgtttatt ttctatattt ttttctaatt tacaaaagta
     3061 atattataat tcagatgtaa gtaaataatt ttaaaattta attttcgata acattctatt
     3121 tagaaattac catattttaa tgtttaaaat gtctgattcc tgttgttacc attttcattt
     3181 cattgtcatc acagtaataa atagataatt tgtctttgat agaacctcca ggttgaatta
     3241 ctgttttaat gccttcatta ttagcgattt caacacaatc aggaaaaggg aagaaagcat
     3301 cagatgccat tacgctatct tttaagtcaa aaccaaaact ttgagcttta tgaatagctt
     3361 gttgtaaagc atcaactctt gaagtttgac cacaacccat acctaataat tgcttgtttt
     3421 tagctaatac aatagtattt gatttcagat gttttgcaat cttattagca aacaataaat
     3481 cttcaatttc agaagaagaa ggtgttaatt ttgtaaccga ttttaattca tcagcaactt
     3541 caatttttgt atcaaaatct tgaacgatag caccatttaa tagtgatttg tattgtatat
     3601 ttggaaaact aaatagcttt tgttttaata agattctatt tttctttttt gtaaaaatat
     3661 ctaatgcctt ttcatcaaat tcaggtgcaa ttaacacctc aaaaaataac ttatcaattt
     3721 caagtgccgt tgcctcatca atagttctgt ttgcaattaa aacgccacca aaagcagata
     3781 ctgggtcgcc agctaaagca gcttgccaag cttctaaaat tgagtttcta cttgcgactc
     3841 cacaagaatt agtatgtttt aaaacagcaa atgttggctc tgtaaactct gtcattaagc
     3901 gaacagcagc atcaatatcg actaaattat tgtaggagag ggctttgccg tttaaaatgt
     3961 caaatacttc ttccaaattt ccaaaataga atcctttttg atgaggattt tctccatatc
     4021 gtaatgcttc accatcatta taactttgtt tgaaaatatt ttcatcttta ttttctgcaa
     4081 aatatctata aattaaggtg tcgtagtgag aagaaactgc aaatgcttct ttagcaaata
     4141 gctttctagt ttctaaagca gttgctcctt tattttcagc taataaattt gcgatagttg
     4201 catattggct tttattagaa actatcaaag tatctttaaa gtttttagcg gctgctctaa
     4261 ttagtgaaat accgccaatg tcaatttttt caattatggt ttgttcgtct gtagtggaag
     4321 cgactgtttc ctcaaatgga tacaaatcca caataaccat atcaatttca ggaatttcaa
     4381 attgtactaa ttgttgttgg tcttgttcat tatctctacg tgctaaaata ccaccaaata
     4441 tttttgggtg taaggttttt actcttcctc ctaaaatttc aggaaaagca gttagtcctt
     4501 ctacagtatt tactttataa cctaatgatt ctatgaactt ttgagtacca cctgtagagt
     4561 aaaagtttac agcttgtttt gccaaaatat gtatcaactc atctaatcca tctttgtaat
     4621 aaacagaaac aagaacattt ttaattgcct tcacgatata acttttttac aaaagtaagc
     4681 aaattataga cttatatttt gtttaggtat tttattaaat tgtattatcc taaaaaatgg
     4741 tgtttttttt agaacgatac acttcgccat tgttggtttg taaccaacaa caccattttt
     4801 tttgcggaca ggtaataaca cccgtcagag gcaagggaca atacaagatg ctatttacat
     4861 taaaaatcgg tcgtgttatt taaaatattt tgttgctaat tcgccaatca tcatttaaaa
     4921 tatttttcca gtcagagtct gtcaataaaa accattcgcc tgttcgtttt ttagattcaa
     4981 agattctgtg aagtgttcgc tctgttttag ttccacttgc tttgaagaat ccaactatat
     5041 caataggttc aggatttcca gtttgtaaag atgaaattct gatttctact gcttgtttgt
     5101 ctgtcgagtt gtttttcctt tcagtccagc caattttaaa ataatttgta tttcgttgcc
     5161 ttacaatata aattagcccg accgttgtat cttcttcaaa aagtcgaaaa atatgattat
     5221 tatttttttt aaattttttg tatgagtaaa gccaattatt ccaaatttct ctatctgatt
     5281 cacttctgct taatgtatcc gactcagcat ctttcaatat tcttttaatt tcatcaattg
     5341 taagatgccc tctgtccaat tcatgtttaa atagttcctt tgctttctgc aaatctcttt
     5401 caataataaa aatccaatct gattttgtga agtaagcaat acggattcta ataaatggtg
     5461 aattttttaa cccaaagtcg tcaaaaagta aagcattact ttcttttaat ggattcatta
     5521 aaaactttgg aggtaaacaa tttatgtaac ttaatgaagc catattgtat tctttttcct
     5581 tttcggaata tagcttatca ttttcttcaa agtattcttc ttgagatgat tcatgagtgt
     5641 ttgatttcac tttatctttt tgtccatcag aggctaattc aaacaattta aatacttgtt
     5701 tttgcaaatt atattttttt gcaaagtcgt tagtgtagtg tttacaaatt acatttttta
     5761 cttcgcttaa aataggtgag taaatgtatt cgtccaaata ttcttgcatg tctggtcttg
     5821 acagatatgc agcggcaatc caattgataa cttgcttttg aattgttttc aagtcattat
     5881 ttttaaacca gtcgtatagt tctttgttaa tctcaaaggc aaagatttta tagtttgata
     5941 tgccgaaact gaacatacct gtgttaatgg ctttgtgcaa ctcttcgaga atttcccatt
     6001 caatgtcttc acctggtaga aagtttcttt caaggtagca agctctgatt tgttgaacat
     6061 tttttattct atcaaggaac ttgtcttttt tcaatttcgc ttcttgatgg atgtatgggt
     6121 ttattaaagt cagtttgttc atgtctttat atttagataa tttgtaataa agtggagagg
     6181 ggggacacga actccctacc tcaatcttgg caaactaagt cttctaaaat ttcaatgtca
     6241 tgttaatcgg agtcaagacc attttctttt cacgaggaca acccccaact actacataaa
     6301 caaataaaac ttccaaaatt aattctacag ttgttattga agtttgcata atcgagttat
     6361 ttaattgttc aacaaatacg aaaattgaag aaaacgacac aactatgatt taggacattg
     6421 caagttcaat agttaactta atttgtaagt ccaaattttt gtgttttgtc aagcaaaata
     6481 atattttgtt caaagaactt taaatgtaaa cggttatgct gcattacagc caactctcaa
     6541 atttaagaat gtttttttat attcacaaaa aaaatactca ataaaagtgg ataaattttg
     6601 taaaaattac ctctgtatct aggcacaggg ttttgttttt ttagggtctt ttttattcgt
     6661 cctaatttta ttatatgttt tttgtactta ccacttgtca gtattctcca aatttgctac
     6721 tacctgagga agtttttcat ttataaaatt tacccatttt ttttgagaat ttttgcctat
     6781 agtttctagt gcaacagttc cataacgaac atctttgatt ctaaaattat ttattgttaa
     6841 tttatatttt ccttctttga aatcaataaa tgcattaaaa tgtacttcca tagctgaacc
     6901 taaaatcatc ttagtaaaaa agttttcacc tttaatacct ttgtcagtaa tattcacatc
     6961 agaataatta gattctttca ttttttgagc aaaaagatct aatcgagtat tgataccatc
     7021 tttaaattct tttatatgtg agtattcata aatgcctaat gtctcattca tagaaacgtg
     7081 ttgtccataa caaattgtag taatcaataa tgaaagataa ataaataaat gtttcataat
     7141 gtttttttgt ttaaaatgta taaaaaagac ctgttcaagc gacacctacg gtgagcgttc
     7201 tcctaagtta cgttacaaca ttcaaatata agatttctat ctttaaagtt tataaaaact
     7261 ttttaaatat gaaaggaaat acaattatac taaaaaaaac actcaatgaa agcaaataat
     7321 ttaggtagga atttagtttt taatgagtaa atctatttca agaaaagaca caataaacgg
     7381 attaagtatt atttttgaac ataaaaaaag ccaatcaaaa gattggcaaa tatttaattt
     7441 ataaatctga aagttacaga cttaattcaa ttctttttct taattcatct actgaatctt
     7501 taaaatcttc atcagtatct actagatttt tcacagcttg gcatgaatgg ataaccgtac
     7561 tatggtctct gcctccaaat tgttgtccaa tgctttttaa tgatgctttg gtaaattctt
     7621 tagcaaaata catcgataat tgtcgagctt gtacaaattg tctttttcta gttggttctt
     7681 ttagtttttc aactggaact tcaaaataat cacaaacagt tttttgaatc atctctaaag
     7741 aaatttcttt agttgcatgt tttacaaaac tcaaaactgt tcttttggct aaatctaaat
     7801 tgatttcttg tttattcaaa gtagcttgag ctaaaatgga agttacaaca ccttcaattt
     7861 cacgtacatt atttttaata tttgttgcaa taaatctaac aacgtcttca ctcatttcaa
     7921 caccatcagc atgcattttt tttctaagaa tagaaactct agtttcataa tcaggagact
     7981 gcaaatcagt ggttaaaccc catttgaatc tggataataa tctatcttca acaccttcta
     8041 aatcttttgg agctttgtca gatgttagta ttaattgctt gttattttgg tgtaaatggt
     8101 tgaaaatatg gaagaatata tcttgtgttt ttgctttatt tcctaggtaa tgaatatcat
     8161 caataattaa aacatcaatt tgttggtaaa aatggataaa atcattcgta ctttgttctc
     8221 tgagtgcatt caaaaattgt gttgtgaatt tttcagaacc tacatacaat acagtttttc
     8281 caggtgtatt tgttttaata aagtttccaa ttgcttgagc taagtgcgtt ttgcctaaac
     8341 cagttcctcc gtgaattact aatggattaa aagcattacc accgggtgat tttgctactg
     8401 cttttgcagc atttcttgca aatttattac aatcaccttc tataaatgtt tcaaataaat
     8461 aatttggatt taattgtgaa tctacatgta tcttttttaa tccaggaatt acaaaaggat
     8521 tttttaccat tggagtagaa agcatcgttg gaagtgatac ttcatttgct ccatatatac
     8581 cttctctatt attaggtaaa tccattgttt ttggatttgt atttgcagcg tttttagact
     8641 ccattaatac tcggtattct aacttcgctt gatctcctaa ttctctttta atagtttttc
     8701 taagtaaggt aacataatgt tcctctaacc attcataaaa aaattgacta ggtacttgta
     8761 ttgtaagtac aaaattttcg actttaatag gttttatagg ttcaaaccaa gttgtaaaac
     8821 tctgtaagct aatattatct tttattattt ttagacaact ttcccaaacg agttcatgct
     8881 tactcttcat atttcattag ttttaaagat gtgttgataa ttacattgct aatatggaaa
     8941 acttttttgt aataaaaaat ttttttttag ttgtttttta tgtaggaatt acaatatggt
     9001 ttttagcttg tttttttgta gatttttttt caaaaatata atctatctga ccaagaaaaa
     9061 taccagcaaa ccctacttga tttatcagta catctttatt ttctaaattt tgtattattg
     9121 ttggttcttc taaaaaagta tgtgtatgtc caccaataat aatatcaatg tcttttgttt
     9181 tttgagcaag ctgtaaatcg cttattttat tggtgttata agcatatcca atgtgtgata
     9241 atacaataac aatatcacat ttttttttgt gttttaaaat atctacttgc tcttgtattt
     9301 tgtctatagg atcatgatat acaatgcctt cgtaattgta tggtgcaact aaatttttta
     9361 attcaacacc acatccagta atgccaattt ttaaagagcc tttttgcaga gtaatatgtt
     9421 tgtgaatttt accatttaga agcgtatttg taaagtcata attacagttt acaaacggga
     9481 aatttgctct atcaatttgt ttagctaaag catcaatacc attatcaaat tcgtgattac
     9541 ctagtgtaga acattcatat cctaattcat tcattacatc aatttctaca ttgcctttaa
     9601 aaaaattaaa ataaggtgtt ccctgaaaaa tgtctccagc atctaataat agtgtatgtt
     9661 cattttctgt tcttatttta ttaattaaag tagaacgcat ggcagcacca cccataccag
     9721 gatattttaa atcagttgct ggaaatggct ctattcggct atgtacatca ttagtatgta
     9781 atatagttag ttgtttactt ttattagaag agaaaatagg ttggtgtaat gcaagtgcag
     9841 cagttgttgt gattgctgtt tttaaaaaat tacgtctatt ctgcatacat tattcttttt
     9901 tgatttgaag gaataatggg ttctttggta ttttttaaat attcgatgat ggcatctcga
     9961 aataaaacat tgaaatttat ataaggtttg tcttttaaaa atgacatatt atctcctcca
    10021 ctcactaaat aatcaataat tgctacttta taaattttat tttcttgtaa tggtttttga
    10081 ttgattaaaa tatcaatagc ttttccatct ttgattctaa aattaatttg attacttatt
    10141 ggccaaccac ctttttgtgc aatcacatca cacaacattt gcacttcttc ctgattgata
    10201 cttaaaacgc caatagtatt ttcaaatggc ataatattaa aggcatctaa aatttttaaa
    10261 gttccctttt gaagtgtatt aactctgatt cctccataat tagtaattgc aaaatctaca
    10321 gtatcttttg acaatttttg ggtttcgtca aaaagaatat ccgcaattaa atttcctaaa
    10381 gttgaacttg gttgttgttt gattacattt tcagataaaa ttgcaatagg taatagcata
    10441 attttatcta atgaatcttt atatggttta tataaattat aaatactgga atctaaagca
    10501 atattattta tttgaatatt atcgtaatgc ttagtgctaa cttgataatg cttacaagaa
    10561 acagtcaata agataaggaa tattaaacca tatttaaaat aagttcttaa catcttcttt
    10621 gataaaattg atagcaatat gtgttggaaa ttcttgatta ctttcagcta aatactgaaa
    10681 aattgctttg cttaattcaa ttccattcgc ctctttgggt aaatttgaag ctatactatt
    10741 tataattttt aaagtttcat cttttgctct ttctactaat atccacgcat cttgattaag
    10801 gtaaatttgt tgtgataaat tatattcata ttcaattcta atggcttttg ctaattctag
    10861 atttaaagca gcaacagtca tgttagcttc tctaactctt tgaaccaatt ggttgggatg
    10921 gatacgttct aaaaataaaa taacacgttc atacgcttgt aatctcaatg gcaatgcgat
    10981 tttagaattt tcttgaatac ttaatttttt tagttctaat tttttaagtt tttcattttg
    11041 ttccaaaaac cttactataa ttaggtaaat acctataagc ataattaatg atggtaatgt
    11101 gtattttaag atttcaagaa aaaaactcat attatatttt ttttgcaaat ctactatttt
    11161 atttaatatg cttttctaat tgttattaaa tatcaaattg cgatttaaga attaaaagat
    11221 acttatcttt gtatcaactt aaaaatatta aagtgaaaat ggaaaataca tctagtttgc
    11281 caattaatat tacaaattct gcactagaag aaataaagaa tttataccaa caaattgaaa
    11341 ataaaactga aataggattg cgtattggcg tagaaggtgg tggttgtgct ggtttctcat
    11401 atattatgaa ttttgaaaaa caaaaagaag gcgataataa atatatatta gaaggcttaa
    11461 ttccaattta tatccatcca acacaagaaa tttatttatc aggcaccatt atcgacttta
    11521 aaacaggttt agataataga ggatttgtct ataataatcc aaatgctaca gaaatttgtg
    11581 gctgtggaac atcattttca gcttaaaaac catataaata agctattttg atataaaatt
    11641 gttcacaaag cacctatttt attaacattt ttattttttt tcttgttaaa ttaaagtaac
    11701 ctattatatt tgtgaggtaa tatttattaa acctatttaa acaaattaat atgaacaaag
    11761 gagatttagt taaagttatc gctgatgccg caggcgtaac aaatgcgaaa gcaacagatg
    11821 cattaaatgc aattttagac ggtatgggaa aaacacttaa aaaaggtgac aaagtaactc
    11881 tagttggttt tggaactttt tctactagca aaagagctaa gagaattggt agaaacccat
    11941 ctactggcga aaaaattact atccctgcaa aaactacagt gaaattcaaa gctggtaaag
    12001 aattatctga atcagtaaac aaaacaaaaa aataattagc attaattatt tttatttaat
    12061 cccacattta ttgtgggatt ttttatttat atattttaaa attattcatc tacaatggca
    12121 ttaaattata tttggattag tttttttgtg attagtttta ttgttgctat tgttaaaaca
    12181 atactcggcg atactgaagt attctcattg ctgaccacta aattatttga aagtacaaaa
    12241 attggatttg aaattgctct aggcttaacc gcattgatgg cattttggct aggtattatg
    12301 aatattgccg acaaaagtgg attgataaaa aaatgttcta atttattagc tccatttttt
    12361 tcaaaactat ttcctgaagt tcctaaagaa agtcctgctt atggcaatat gatgatgaat
    12421 ttttctgcca atatgttggg tttagacaat gctgcaacac ctttaggttt aaaagcaatg
    12481 aatgatttgc aaaatttaaa taatcaaaaa acagttgcct ctaaagccat gatcatgttt
    12541 ttagtaatca atacagcagg tattacacta attccaactt cagttatggc cattcgtcaa
    12601 acaatggcta ttgagcaagg cttagtaggg tttaatgcag cagatatttt tttacctact
    12661 ttgatggtta cgtattgtac actatttttt gcaattattg cagtggcttg gcatcaaaga
    12721 ttaaatttat tcaacctgcc ttttattact tttaatttat tatttatagg aattatttca
    12781 ggtatgtact tttggattaa acaatttcct caagaagcaa tagctggaaa aattgcacta
    12841 attggaagtg tattgatttt tggaattatt gtagtaatta ttatttctgg tgcaattaaa
    12901 aaaataaatg tttatgatgt ttttattgat ggagcgaaag atggttttaa aactgcagtt
    12961 caaattattc cttatctaat tgcaatcttg gcagccattg cagtttttaa tgcaactggt
    13021 tgcttaaatt ttattacaga tggaattggt aatattgtta ggctcttagg ctttgatgat
    13081 aaatttattc cagcattgcc agttggctta atgaagacac taagtgggag cggagctaga
    13141 gcctttatgg tagatgtaat gcacaattac ggtgtcaata gttttcaagg aaatttagcc
    13201 tctattatgc aaggctcaac tgaaactaca ttttatgtat tagcagttta ttttggaagt
    13261 gtaggtataa aaaatgccaa aagtgcaatt ttctttggac ttttggcaga tttcgtagga
    13321 ataattattg caattgcagt agcttattta ttttatagct agaaaaagtg ttttattgaa
    13381 tacccaaatc ttttccatca gtaagccatt tcatacttat cgatacaaaa gtattgtctt
    13441 tgtattcatc tttttcaaaa attaaaccaa tctctccatt ctttaataaa gttaatgaag
    13501 aataagcagc accacctgta taaatggttt taccagctga ccaagtttta ccttcatcat
    13561 aactgattct aatggttaag tttttacgtt gtttagcatc ttttgcgtta acaaaaatca
    13621 atctattttt atcatcgcct tctgcaacag aactatatct taaaatacta gcattgcaac
    13681 ctggatcagg taaagctggt gccgaaattg tagtccaaga taaaccttca tttgtcgaag
    13741 tatgaatata tctataacca actttattta cacgactatt tatcatccaa gtaccatcat
    13801 tcaactcaat aatttttgat tcatcaccag gtgtaataga tttgtctatt aagtaccaac
    13861 tatcaccatg attatcgctt ccaaatacat ataacccact atttagattt acaatagtgt
    13921 gtaataattt tccatctttt gtttgtgttc catttccaga tgtaatgaat ttaaaatgat
    13981 tgtgccaact agacttagta acttgagaag taatatctac tggtgtactc catgtttttc
    14041 cattatctga acttttcgtt accataaaat aataaatatc cttttctgta tttaagttca
    14101 tataattata aaacataaaa attgtattgg tagttttatc tacaataaat gaaggatcag
    14161 aagctgattg accatttgga taatctataa ctttttcaat tgctgtccat gttttgccat
    14221 tatcagaact acgacgaatt aaaatattaa tatctttatt ggttcttaaa tcaccacaat
    14281 ctggaacacg ttcatcaata gcagcaacta aatcgccatt tggagcagta gtaattgttg
    14341 gaattctata acaagaaatt cctgttgtgt tttttgtatt aaaaatcgaa gtaaaggtta
    14401 gaccagcagg tacagtgcct ggagcaattg tacatgaaat aaatgaaatt gctgaaataa
    14461 aaaagataat taaaattctc attattggtt atttatgctt gtaattttat aaattagtct
    14521 gtcttgaact atttcttctt tttagaagta ctaaagtgat taatattatt atcataataa
    14581 aaagagctaa aggaattatt tgaaaactac cttagcaaat aacagttagc taaaatcttc
    14641 agcaataata ttttccatat ttctttctgc aatagcagca atagtcaatg ctggatttgc
    14701 acaacaagaa aaaccaggca tcatagagcc atctaagcaa tataatcctt catagccttt
    14761 caatcgtcca taataatcac ttgctaatcc aagacaagca ccacctaatg gatgataaga
    14821 gaaattatct ccaaagctat tgccattaaa ataaatattg gataatgaac ctccattagc
    14881 ttcatttaat ttatcaataa aagcttgcat tcctcttcta gaaatatcca tttgtgattt
    14941 gtcaaaatgc aattcagccc tatcagtgtt tggatcatat tcaaaatatc ctctttctgg
    15001 attttcagta attgctaatg tcaataattg ttttaactct ataccaattg gtaatggtgc
    15061 ttgttctgct aaagttggtg ttactgggct atccaagtaa ccataagcaa caactggaac
    15121 agcaccttgt acattacctg tgttttcttt caagcctgtt ctcattgcca taatatttcc
    15181 atttccgccc caatgtttac ctacttcatc acttaaatta gataagccac ctttgtattt
    15241 cgattttaaa aggatatcca ttgtgccagc aacaccagca ttaataaata ctttttttgc
    15301 gatatatagt ttcctatcta tcttttcgcc tacttcatca ataatatcca caaatacttc
    15361 atatctacca tcagaacgtt tattgatata atctacttta ctcattgtct gcacagttaa
    15421 atttccagtt gcaattgcag caggaatata atttctatcc aaactgtttt tgtatccatt
    15481 gtatgctccg tatattacat cgccattcat acaacatttc ttctctgttc cgtctaattc
    15541 tcctttgatt acatcaaaat taacaccagc atttaagaag attggtgtca tgcctgcttt
    15601 atcacaatgg tctaaagcaa ctcttgcata atcatacatt tttgagttta ataattcatc
    15661 tggaacagtt gaaaaatcaa gtacactttt tactttagga taccacttat cagtcatatc
    15721 ttggtagtta atatatggga aatatttatc ccaaagctct gggcgtgcat aaggcaacat
    15781 ggcaccataa acaacagaac caccacctaa acaagttcct ctatagattc tagtatatga
    15841 agtatcataa tcgatacgat ctaaaacacc tacatatttg tagatactaa attgaagaaa
    15901 atcattaact actggaacat tagttttctt ttttaaccaa gaagaacgtc catctggttg
    15961 taacgattta gcaaaaacat cttcatattt cgaaacagta tattgtctgc ccatttccaa
    16021 catcagggct tttacaccgt gttctgctaa acgcaaagca gatatagcac caccaaagcc
    16081 agatccaata attacgacat cttgttcgct acttggaacg ttttcaactt tgtctttttt
    16141 gcaacctgct aatgaaaaag ttgttccacc cattgttgca atagttgcca ttgctgaaag
    16201 accaacgaat tttcttctgg taagatcatt attactcata aatatactat ttattgatta
    16261 gtgttttgta attagccaaa aatagtgata tttaataata tctaaaaata cattatcata
    16321 tttttaacaa aaaagatgtt tgaaatcatc tagtttataa aacctttttc acgcatccat
    16381 tcgtcgttaa agaattttcc tacataacga gaaccatggt cgtgcgtaac gattattacc
    16441 aaatcatctt tagttaatgg aaactttttg tgcaattgca ataagccttg cacagcagaa
    16501 cctgcggaat tacccaaaaa caaaccttct tcgacggcta atcttctttg catcaaagcg
    16561 gcttctttgt cagtaacctt ttcaataaaa tcaatcaaac tgaaatcata attttgagga
    16621 ataatatcct cgccaattcc ttcggtaata taactatgaa tttctgctgg atcaaaagtg
    16681 ccagtttcgt ggtattgttt taaaatagag ccataagtat caatacccca agtttttata
    16741 tttggatttt gttcttttaa atattttgat gttccagtta cagttccacc agtgccaatg
    16801 cctacaacaa aatgtgtaat ttttccatct gtttgcttcc agatttcggg gcctgtgctt
    16861 tcataatgtg cttgtctatt ggataaattg tcgtattggt tcatccaaat tgaatttgga
    16921 atttcagtcg ctaatctttt tgcaatcgaa taataggagc gttcatcatc tgcacttaca
    16981 tttgtaggac aaacaattac ttctgcacct aaagcacgca acatatcaaa tttttctttg
    17041 gattgtttgt cagtagttgt aaaaatacat ttgtagccac gaactgcagc aacaattgct
    17101 aagcccattc ctgtatttcc agatgtacat tcaataatag ttcctccagg ttttaatctt
    17161 ccgtcatttt cagcatcatt taccattttt aatgccattc tatctttaat ggaatttcct
    17221 ggattaccat attcaacttt tacgcaaact gtggcaggta tgtgagctgt tattttgttt
    17281 aattttacaa ttggtgtatt gcctatggtt tctaatatgt tgttataaat ttgcataaca
    17341 caaaggtacg tttattttct tgttatttta atggattatt ccttgaagtt ttgcttatgg
    17401 gtggctttta atgaagtttt attaaaaatg aaatatcaac atctgtcgca cctccagcat
    17461 tcttaacttg attttgtgca acagctaagc cttttttgtt gggttgatgg attaggataa
    17521 ttcggaaata ttctaatgat aatatattat ttgtttttat aagtgttttt aaaccaattg
    17581 gataagcatt attgtcttcg tccatatatt taaaactatc aattgtaata gattgtataa
    17641 aaatctgatg ttcttcagct tcttctaaaa tttcattatt gatactgtcc accacagaag
    17701 cactttgaac attgtaaaaa gatatttcta aactataagt cttattcttc atcaaagaaa
    17761 tggtatcttt tttatttcca aatatgccat catttatgca atacaaaaat gaacttgatt
    17821 cgcctaaagt atctgtgaaa gttaattgta ctcgagttat taattcattt tcattaatag
    17881 gtaagggttg ttttgtacac tgtaatagaa tgacactaga aaaaataagt aatagcgttt
    17941 gcgtagttgt atatttcata atatttagtg attaaggaaa taattaattt taaatagaaa
    18001 attaaagccg atttcatctg tgaagtatcg aaatctatta agataatctc tgtagttttt
    18061 atttaagata ttctcagctg caaatgacaa cattattttg tttgctttcc cgacagatgt
    18121 gcctaattgt aaattaagta gaaaataggc attaggaaca tctttgtaat ccttaattaa
    18181 tttaggtgtt cttttctgct ttagaatata atcaccatta atttgtaggt atgcattttt
    18241 aagaacttta gtatcaccat aaaactttaa tccaaacttt agttggtctg caggaatgtt
    18301 tacgatataa tctttagttt ttaagttttt ggctcttatt aaattagatt gaaactgaaa
    18361 ttgaaaataa tcggttatat tgatttgtgt atgtatctta atattattaa aaaacgcatt
    18421 ggtggattga tattgaaata caggaaatgc acctttgatt gtcaataaaa aatcactatt
    18481 aggctgtaaa tagataaagt tttttaaata ttgcaattta tagtcgatat tcaatagtat
    18541 atattttcta tattttaagt tgaatgcgtt tgaaagagca taagaatttt caatttttag
    18601 cattttattc ccatactcaa ttgttgccgt tccatgatgt aatccattgc taaataactc
    18661 gctgatgttt ggagcttttg aagatagaga aatgcttgtt tggtaatcaa aatatttttg
    18721 taaataactg aaatttgcag taaaattgta ggtattataa tttttcttgt aaataattag
    18781 ttctttctta ttaaaagtat aactatttaa ttgttgattt tcatatcgca ctccgacatc
    18841 aaaagatata ttatttttaa aaaattcttg aatagaaaaa atggcaaaat tatattgtag
    18901 ataatcagga ataattggaa taattccagt atttggatcg ttggtattgt ttttatattg
    18961 gtaaataagt ccactttttt gatgccattt ccaatttgtt tttaaatact tttcactgtg
    19021 atttacgagc tttccgtaat agcttattaa atgcatattt aaagctggag ttgaagaacg
    19081 attacctcta cgaatatcat attcttgtct tctattattt tgaaaattag ttcgatattc
    19141 aataaaatgg tgctttcgta aaggctgtac taatttaata tctaataagt gatgttgcgt
    19201 aaattgtttg ggatttttta ttttataact agctggttca atgataattg gggtatctga
    19261 gttgatggca tttttcaaat cttcaatatt gccaatatgt gcagcaagca aaattccatt
    19321 ctcattactg aataaactat aaattgtttg aattgttgac ttctttatat tgaacttttg
    19381 ttgaaaggat agacttgttt cttgttgtgc tgtattgctt aatgtgtatc ttggagtgtg
    19441 caaatctgca ctttttttaa aagcagtttg tattcggaaa gcatattgtt ttttaaatga
    19501 tttttgaaaa taagtggcta atgaattttg ccaaccgtta ctttgtaatc cataagacaa
    19561 gccaaattta attgcttctt tgtatgaaat caatggattg tctatagtta ttgttgcacc
    19621 gatattttga gtggaatatt cgactgtttg actattattt aaaatctcaa ttttattaat
    19681 attgctaatc tcaatttcag gagcatgatc agtaccccaa tcttgtgatt gaaaaagaac
    19741 accattattt actaatgtaa ttctatcgcc ataaaaacca ttaaacattg gtttgttaat
    19801 gtttgaacct gattttataa ctcttattcc attaatggct tgtattgctt gtgctaggtc
    19861 ttttccagag agttgttgca ttgaaaactt attaatcgta tttgtcatct gcaaattatc
    19921 gctgagttta gcatctgttt tagtgaattc atctaaaaaa taaacaagtt gttctaaata
    19981 aaaagtttgt tttctatcgc ctttaatatg aataattatc gtatctgttt gacaattgat
    20041 atgattaaat tcaaaatggt aagtttgaat acataaatta ggaataacga attttccttg
    20101 ttcatcactt tttatcaatg tttgattttc taatatttta atttctacaa attctaatgg
    20161 cgtgttatac tgattaaata tttgtccatt tagtgtataa taacaatctg aagcgagtaa
    20221 tttatttgtg aaaagtaaaa aaataataga gataataaat cgcattattt tgcaaatata
    20281 aacaaattat ttaaaatgca actgtgttgc attttttgta agcaagaata cccagaaata
    20341 tattactaaa ttaaaataga taaaaatctt ttattttaac tattttttca atgcatgcat
    20401 tgtatttgtc agatagtatg ttatttttac acaaaaaacg aaggatagat ggaggattta
    20461 tttaaagaag ctgctgataa aatggtcatt tataatgtta ggaaatcttg gttgattatt
    20521 gcgaaattat ataatgaaat gactgcaaaa tttggaggta caatatcaat ggctttcgtt
    20581 ttgttggctt tgtacgaaga agaaggtgtg ccagttacta aaattgctcc tagaattggt
    20641 atggaaccca atagtttatc cagaattttg aaagaattag aaagtaaagg aattttagaa
    20701 agaagaaaaa atcattcaga ttctcggata gttaatattg tattaactga acaaggaaaa
    20761 aaacttcgta aatatgcatt gaaatccgta tttaatttgg agaaaaatgt aaaagaagat
    20821 atatctgaaa atgatttaat cggttttttt aatgtattgg aaaaaattcc gtctgcaatt
    20881 aataaaattc aggaaaaagt taatcaacaa agaaataaat aatatattta ccttgaacta
    20941 actaagcatt ttttcttgaa aataatagat atacaaaata taaaaagaga gttcaaatta
    21001 ggagatgaac taattcaagc attgaagggt gtagatttac aaatccaaaa aaatgaatat
    21061 gttgcattga tgggaccttc tggttctgga aaatcaactt tgatgaatat tttaggttgc
    21121 ttggatacac caagttcagg tcaatatttg ttgaataata ttgatgttag tagtatgaac
    21181 gataatgact tggcaacaat tcgcaataaa caaattggtt ttgtatttca gacttttaat
    21241 ttgattcctc gattaagtac attagataat gttgctttac ctttggtgta tgcaggttgg
    21301 aataaatcta aaagagaaga gcgagcaaaa gaagttttag agcaagttgg attagctgaa
    21361 agaatgaaac ataagcctaa tgaattatct ggtggacaaa gacaacgtgt tgcagttgct
    21421 cgtgctttag ttaattttcc atcactgatt ttagcagacg aaccaactgg aaatttggat
    21481 tctaaaactt cgtatgaaat tatggcatta tttgacgaaa ttcagcgatt gggaaataca
    21541 gttgttttag ttacacacga agaagatatt gcaaaataca cacatagaat tgtacgttta
    21601 agagatgggc tgattgaaag cgatttgcca aataatagat aatttacttg aataagcaga
    21661 ttttaaattt ataatttcaa aattagataa aaatgaaagt atatacaaaa actggcgact
    21721 tgggagatac tgctttatat ggtgggcaac gagtttctaa atccgatata aagattgata
    21781 gttatggaaa tgtagatgag cttaactctt cgattggtgt tgttgtttcc ttactgaacg
    21841 aacaaaaatt attacctgaa acagttgaac aattacacga aattcaagaa atattgtttg
    21901 atattggtgc tcatttagca aaacaattag gtaaagaaaa attggcatta ccagaaataa
    21961 aagaagatta tatcaatatt ttagaacaac aaatagataa atggactgaa gaattaccag
    22021 aattaaaatt ttttatctta ccaggtggaa atataattgc tgcacataca catatagcaa
    22081 gaacagtgtg taggagagcc gagcgttcgg cagttttact aagtcatcaa gagtctgttg
    22141 agcctatttt aattaaatac ctgaatcgtt tatcagatta tttatttgtt ttatcacgat
    22201 atattaatta tatttcaaaa tcagttgaaa ttatttggtt aggaagatta agaaaataaa
    22261 atacaaaagt ttttataatt tacattttat tcatttttag aagtacttat atttagtcat
    22321 tttttatagg gtttaaattc ttatagatta tattatattg gttttaagct taattccatt
    22381 tatttatggt aattttaccc attcaaatta tggaaggaaa caattttatt gactatgtaa
    22441 aaatctttac aaaatctggc gatggtggtc ccggttcagc acattttaga agggagaaat
    22501 atgttcctaa aggtggacca gatggcggcg atggaggacg tggtggtcat attattttaa
    22561 gagggaataa gcaactttgg actttattac atttaaaatt tcaaaaacac gttaaagcac
    22621 aaaatggaga aggtggaaaa ggtgcgttga gatatggaaa acaaggtgat gatattattt
    22681 tagaagttcc gcttggaact gttggaaaag atgcagaaac aggtgagaag gaagtggaaa
    22741 ttatggaaga tggacaagaa gtgatttggt taaaaggagg caaaggaggt ttaggaaata
    22801 atcattttaa aacatctact aaccaagcac cgcattacgc acaaccagga ttgccaggtg
    22861 ttgaaggatg gaaaatatta gaattaaaag tactcgcaga tgttggttta gttgggtttc
    22921 caaatgctgg taaatcaact ttattgtctg tattgagtgc tgccaaacct gaaattggcg
    22981 attatgcatt taccacattg gttccgagtg ttggcgttgt aagttatcgt gataatttat
    23041 catttgtgat ggcagatatt cctggtatta ttgaaggtgc ttctaaagga aaaggcttag
    23101 ggttgcgttt tttaagacat attgaacgaa attcagtttt gttgtttgta atacctgccg
    23161 attgtgatga tataaaaaaa gaacttaaaa tattagaaaa agaactaaag ttatacaatc
    23221 ctgaaatagc ctataaaaag aaattgcttg gaattagtaa gtcagattac ttagacgcag
    23281 aattgaaaag tttaatcaaa aaagaattgc ccaaaaaaat aaaatctgta tttttttctg
    23341 cccaaagtgg agatggattg atggaattga aagaccaatt atggcaacta atgaatgaat
//
LOCUS       DAOQCW010000026        23084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_812_length_23084_cov_4.926614, whole genome shotgun sequence.
ACCESSION   DAOQCW010000026 DAOQCW010000000
VERSION     DAOQCW010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 23084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23084
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_812_length_23084_cov_4.926614"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..1275
                     /locus_tag="PLS16_03805"
     CDS             145..1275
                     /locus_tag="PLS16_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HNJ60238.1"
                     /translation="MKSPIYFDNAATTAIAPEVVQAMLPFLNEHYGNPSSVYSLGRDT
                     RAAIETARKTIAQILNAQTSEIVFTSCGTEANNMAIKGAVIYHQVKRIITSPIEHHCV
                     IHIADYLKEFHQVKIEYVQLLSDGMVDLEHLESLLKDNQDKTLVSLMYANNEIGNLLD
                     FEKVSQLCKTHQALFHSDTVQAFGHFPIDVQKVEVDFLSASAHKFHGPKGIGFLYQKK
                     KSKVASFLHGGGQERGYRAGTENVYGIVGLGKAAELAYQHLEKDRQYIQSIKDYFKEQ
                     LLANFKDIQFNGHSKNSLFTVLNVSFPENDAASMLLFNLDIEGICASGGSACNSGAST
                     GSHVIDAIRPNSNRINIRFSFSKYNHLSEVDVVIQVLKKYFS"
     gene            1294..1974
                     /gene="truB"
                     /locus_tag="PLS16_03810"
     CDS             1294..1974
                     /gene="truB"
                     /locus_tag="PLS16_03810"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNJ60239.1"
                     /translation="MDFVAGEIILIDKPLDWTSFDVVNKLKFKLKYQYGKIKIGHAGT
                     LDPRASGLLIVCTGKKTKEIEGIQNQNKVYTGSFYLGAETESYDTEKPAINFQSIEHI
                     TEQEIHKTAESFLGEQQQFPPIHSAVKIDGVRAYELARIGLNPEIKSKVIQIYQFKIT
                     KIELPYIYFYIECSKGTYIRSIANDFGKRLNNVAYLASLRREKIGNFSIEEAYTVDSF
                     IQKLDAIQ"
     gene            complement(1957..3021)
                     /locus_tag="PLS16_03815"
     CDS             complement(1957..3021)
                     /locus_tag="PLS16_03815"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNJ60240.1"
                     /translation="MNHLEYKQLTINGHRLVDEEIIEFCKKSNIENINKIGDFMVEWF
                     SPKTTIEVQTSGSTSLPKTITVSKQQMLQSAKATAHFFNFKENQTALLCLPMNYIAGK
                     MMLVRALYSQLNLICIEPNSQPLNHFIANTIIDFVPLTPMQLKGTKQTSNIKTILLGG
                     GPVSAELEQACQTLSAEIYHGYGMTETLSHVALRKINGSKPSLAYQALPGVLCSIDNR
                     QCLQLEVPFLTEKIYTNDIVDLINETTFIWKGRLDNIINSGGIKLFPETIETKLSDYI
                     KTAYFIAGLPDDTLGEKLCLLIEDEKYTSEKLHHLEQYLEQYLEKYERPKTIFFLPKF
                     IRTESEKVQRSKTIELLNSI"
     gene            complement(3018..4058)
                     /locus_tag="PLS16_03820"
     CDS             complement(3018..4058)
                     /locus_tag="PLS16_03820"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HNJ60241.1"
                     /translation="MNCYFKKYALKFKQPGGTSRGVLYEKETYFIFLQNEEKTAIGEC
                     NRFTKLSYDDRPNYEAKLAKICSRLPDEMEAVLQELTEWPSIFFGIEMLLKDWGNGGN
                     RIIFPEAINENGFTIPTNGLIWMGSKESMLQQIKLKLDANYKSIKLKIGAIDFDTELE
                     LLQYIRKQFSVDEVEIRLDANGAFIYQEAKEKIKQLSDFGISYIEQPINTGQWQDMAA
                     LCNNSPIKIALDEELIGIQNSSKRKALIDTIQPQILILKHALIGGFYAADEWKKLIEN
                     NGGTWVITSALESNIGLNAIAQYTALKCSDYAQGLGTGQLFTNNFPAPYSIDANGLHY
                     HKNQKWDLSALI"
     gene            complement(4078..4980)
                     /locus_tag="PLS16_03825"
     CDS             complement(4078..4980)
                     /locus_tag="PLS16_03825"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: protein motif:HMM:NF004750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HNJ60242.1"
                     /translation="MASISAWLVSFRLRTLPLSLSTIILGSFLATYRNSFQWSVFILA
                     VITTLFLQILSNLANDYGDTISGVDNQERVGPQRSLQSGAITLKQMKLAIVTFVVLSF
                     VSGIALIVVGTRGMEFSYGLIYLLLGIAAIAAAMKYTMGKNPYGYSGLGDFFVFIFFG
                     IVGVMGTFFLHTHEISGLEFLPAISIGCFSTGVLNLNNLRDRINDKAFGKRTMVVKLG
                     VEKAKIYHAILLGIGMLTAIIYTFLAGASLWNWIYLIAFIGIIKSITTVLNNKTPELL
                     DPELKKLAISTLLFSILFGLGLIV"
     gene            complement(4986..5828)
                     /locus_tag="PLS16_03830"
     CDS             complement(4986..5828)
                     /locus_tag="PLS16_03830"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: protein motif:HMM:NF005637.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HNJ60243.1"
                     /translation="MSKIKWQTIKEYEDITFKYCDGVARIAFNRPEVRNAFRPETVSA
                     IQEALDICHESQEIGVVLISGEGPSPKDGGWAFCSGGDQRVRSKTGYKGGDGINRFNI
                     LDVQRQIRFMNKVVIAVVPGWAVGGGHSLHVVCDLTLASKEHAIFKQTDVNVASVDGG
                     YGSAYLARMVGQKRAREIFFLGRSYSAQEAYEMGMVNAVVPHEELEDTAFEWAQEVLT
                     KSPTAIKMAKFGFNLIDDGLVGQQVYAGEATRLIYTTEEAEEGRNAFLEKRAPNFNKF
                     PKFP"
     gene            complement(5833..7530)
                     /gene="menD"
                     /locus_tag="PLS16_03835"
     CDS             complement(5833..7530)
                     /gene="menD"
                     /locus_tag="PLS16_03835"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HNJ60244.1"
                     /translation="MELSDKKGVQQIVQSLAALGLKEIVICPGSRNASFVISFNRHPA
                     FNCTSIRDERTAGFYALGKAIELQEPVAILCTSGSAALNFAPAIVEAYYQRIPLIVLT
                     TDRPKEWTNQGDGQTILQTNIYTNYIRKSYEINGDAVQKNDLWYIERCISEGFNIATL
                     IDKGPVHFNIPVSEPLYGTSSIDTNNLPKIFKAIPLEKQLSKTYLEQLANQFSSSKKV
                     LILVGQLYPNQKLQYLLSKIAQLENVIILTESTSNLHHPDFVENIDRCITGLTAEDTQ
                     KLMPDLLISIGGAIVSKRIKKLLRDYAPTFHWNIDEFDSMMDTYQSLTDAIALQPTIF
                     LQQLLEQIKPIASTYKSTWIALKNQKEQLHQAFCKQIPYSDFSVFNAIYKTITPTISV
                     HIANSSAIRYAQLFDNSKIKCTWSNRGTSGIDGSSSTVVGSAASAPDKDFLLISGDIS
                     FHYDKNAFWIDEQIDNLKVIIINNSGGGIFRIIPGPDKIAEREQFIETSMHTNAEKLA
                     EHYQWNYLSVKEENTLESTLIQFFNKTTKRTILEIFTDAEQNPIILEQYWKLLKDIK"
     gene            complement(7514..8614)
                     /locus_tag="PLS16_03840"
     CDS             complement(7514..8614)
                     /locus_tag="PLS16_03840"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HNJ60245.1"
                     /translation="MERFVISIARILFSKNIPFCIYRFPNEQRISIALDKSLLESNNR
                     KTFWFAPFITNNSYEDIFFDVLNDNEINESFLNFVKSLPQQKEIESNLPNATTKTQYF
                     HRIQLFLEEIHKGNLDKAVLSRVILEDKPQDFNPIDFFLKLAKDYPDTFVHFSQHPTS
                     GIWIGATPELLIKKQDNKFYIMALAGTQARHKVGLEYKWHSKEIEEHQMVNQHIEQVL
                     KKYACAVIDKKALQTVESAQVAHLKTDYIFEETKKIELASLLKDLHPTPSIGGLPVKN
                     GIDCILQHEGYNRKYYCGFIGETNFSETTNLYINLRCLQVGKDHLAIYVGGGITAASN
                     PDDEWKETILKSKTMSEKLQSNTIKLENGIVG"
     gene            complement(8789..9865)
                     /gene="aroC"
                     /locus_tag="PLS16_03845"
     CDS             complement(8789..9865)
                     /gene="aroC"
                     /locus_tag="PLS16_03845"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNJ60246.1"
                     /translation="MAGNSFGEIFRITTFGESHGVALGVVIDGCPAGIEMDTAFLQNE
                     LDRRKPGQSAIVTQRKEADEFELLSGVFEGKTQGTPIGIIIRNEDQKSKDYDHIKNAF
                     RPSHADFTYQEKYGTRDYRGGGRSSARETAARVVAAGIAKLILKQYGIQIQAYVSRVG
                     HLQLAQNYTELDLSNTENNIVRCPDNNMAEQMIEYIKQIRKEGDTIGGIVSCVIQGVP
                     VGLGEPVFDKLHADLGKAMLSINACKGFEYGSGFAGLDLKGSQHNDLFYTEEDTVKTK
                     TNYSGGIQGGISNGMDIYFNAAFKPVATIMRDQVSIDTEGNEAIVSGKGRHDPCVLPR
                     AVPIVEAMAALVIVDHLLRNKIYQ"
     gene            complement(9943..10173)
                     /locus_tag="PLS16_03850"
     CDS             complement(9943..10173)
                     /locus_tag="PLS16_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60247.1"
                     /translation="MAKLTKVLGAILSFVGSVILLYSFFVYLNTSAHLNYNVAKQPSI
                     IDVHMVYPFLIGFITLVFGILFVNDKVLSNKH"
     gene            complement(10244..11491)
                     /locus_tag="PLS16_03855"
     CDS             complement(10244..11491)
                     /locus_tag="PLS16_03855"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNJ60248.1"
                     /translation="MDSLDSLILEEKDLPHGRQNEFLIVILLAFITYLCTVDSMLLMP
                     MINIVMDTYKIGAKTATIIVSIYSFAAFISGIFSSSFMDLYDRKKLLLITFIGFTIGT
                     GLCSMHSHFGLMIAYRAFTGVFGGIIGGIAIAIVSDIIPYERRTTAISIISLSFAIAA
                     ITGIPFGIYLAQHYTLFTPFLWLTIISIFTIFAIILWIPPMKGHINQDNTKKISYSNI
                     KDVFKDSNQVKALLLAFFLVFSHQVVVIFVVPYFESNIGFGEDWIPILYLIGGIVTVI
                     SSIIIGKLSDKYGNKPSFSILIFLSFFPIIVTTHIAKANILEVIIICVFFFIFAAGRM
                     IPAYTIMSAAMVKEKRGAFMSMRSAFLELGTFIASVCSSFVVTIDKNTGWVHHYDRVG
                     YISVIAGVICWILAYQIKIVSKD"
     gene            complement(11545..12690)
                     /locus_tag="PLS16_03860"
     CDS             complement(11545..12690)
                     /locus_tag="PLS16_03860"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF003705.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HNJ60249.1"
                     /translation="MLDTNSLKANFHTYFGYTATDTFFSPGRINLIGEHIDYSGGYVL
                     PAAISLGITAIVGRRKDNIINVYAQNFEEAISIDLQKPLNLNKNIGWARYITSTLQVL
                     LDKNTPLLGVDILIDSDLPLGTGLSSSAALECLIAYIFNEIYYNKNRSELALDAQNAE
                     VKYVGVNCGIMDQFAVANGKKNNAILLNCDTLDFEHIPLDFGDYQLVIINSNTPRTLA
                     DSKYNERRSQCDAAFQILKQFDVAENLCQVHGISLAYLDDDILYKRAKHAVAENNRVL
                     LATDALAQNEHEIFGDLLKQSHHSLSKDYEVSCEEIDALIHIIGHFSGCIGARMIGGG
                     FGGCVIALVEKNSLDKFIEYISVNYERKTKLKAEFYPCHISDGISRI"
     gene            complement(12694..14502)
                     /locus_tag="PLS16_03865"
     CDS             complement(12694..14502)
                     /locus_tag="PLS16_03865"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HNJ60250.1"
                     /translation="MLNVKDAYHQKLLEINGYFTQQILDNDLAEKIKSIYFANFDDKT
                     TTFYSTSFYPDFELKQKVNQEILNIIQPVLDKNFADYKILGSSFLRKLPNQNNPLPLH
                     QDWTVTDEKKYGSYTIWIPLQDTNQTNGAIRVIDRSHQIDDSMRAPSLPVSFEKERPA
                     FDKYLKTLTLKTGEAFIFNQKLMHASWPNQSNEERLALTIGLVPKEANLFMLYFDKNK
                     GEIQQYSMPDDLFLHYPEIIQQPLIGQLEKTFSYQVPQLSEKQFIQNLNAHCQKYSIM
                     EPLFSNEAHQQFFEQNGYIKLPALEQTEIKTLIDFLYNSGIKKENDFGFYVGMDHEDK
                     TLVAKMMHTIEAVAMPKIAPYLCNYQLITASYVLKDPNPKGVVPPHQDWSFVEDENRH
                     CSVTCWIALVDTDMENGCLGVIKGSNKFFNSVRPSPSPQVPSPLAKHMFSMFPYFQLL
                     PMKAGEALIFDNRTFHASPPNITNNARLAIGLSFTQKNAELRHYYLKPGTKDRLLKYK
                     VDPIFFMKYDNGTLSKMYDAGKLIEGYELMEEIPYKWQDLSKKEMKDMILSAGNQHNK
                     ELTQHMTKLFGNYMKENVRDKIKNIIHTFNPLRFLK"
     gene            complement(14502..15425)
                     /locus_tag="PLS16_03870"
     CDS             complement(14502..15425)
                     /locus_tag="PLS16_03870"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HNJ60251.1"
                     /translation="MRNVFKDAALESQFQKDGYVIIPFLNEEEVEFLKNKYFETLEQS
                     GGNIGEQDANLKERITYDFTFINKNIDYKRMVYNLISEKFKPHYDKYLDNYRPVIANF
                     IRKQSNDGEVPLHQNWAFVDERKVTSVSIWCPLVDSNSQNGTLEVTPGSHKRFGEVRG
                     PMIPWELENIKHDIINKHMIPMNLKAGQAVVLDDSLVHYSNINQTDGLRLAIQLILIP
                     AEEKSIHYHMNPQKSSSKVDVLEVDVDFYMEFNPWKQPENAKVLRTFNYKPFNLSLKE
                     YEKGLFNTRIDETPVIEEKPSFFEKIKAVFN"
     gene            15479..16372
                     /locus_tag="PLS16_03875"
     CDS             15479..16372
                     /locus_tag="PLS16_03875"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNJ60252.1"
                     /translation="MWPFSKSKKNKKNTADHAIDVGNFYDKNHSAFIQVYGEVIQAFR
                     TNNLDSILNYQIHQIGLKPEYKVLDAGCGVCGPARYFAKNTGCSIDAVSVSATQVNSA
                     KAYIEKENLQDKISVHQYDYHEVDKLFPKENFDKIYFLESFGHSTDKEKLLKAMWKVL
                     KPGGEIYIKDLFRRISSSWLVQRKIDIEINKINKAYHYEVADLSEFVNVVREIGYVIE
                     FVKTIDIPLDEFENLSISNEFQELTGIAKIDNWEKYIFPIDFFEVKLYKPEYDIEKGK
                     GRYFLQNIYQIQMNHLNKEHL"
     gene            complement(16373..17944)
                     /locus_tag="PLS16_03880"
     CDS             complement(16373..17944)
                     /locus_tag="PLS16_03880"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNJ60253.1"
                     /translation="MNYLSHMSSINQKLEELYQKEQEALLGGGQKRIDAQHKRGKLTA
                     RERIELLVDKGTFEELDKFVVHRSTDFGLDKEQYLGDGVITGYGKVNNRLVYIFSQDF
                     TVFGGSLSETYAEKICKIMDLATKVGAPVIGLNDSGGARIQEGVVSLGGYADIFHRNV
                     QSSGVIPQISAIMGPCAGGAVYSPAMTDFTMMVEHTSYMFVTGPNVVKTVTHEIVSSE
                     ELGGAMTHATKSGVTHFTAKNEVECIMQLKQLLSYLPQNNQEKTPKVDYAQKVSEKIE
                     ALKNILPENPNQPYDMRDVIALSVDEDSFFEVHKGFAENIVVGFARIAGRSIGIVANQ
                     PAYLAGVLDINSSRKGARFTRFCDAFNIPLLVLVDVPGFLPGTDQEWNGIIVNGAKLL
                     FALSEATVPKVTVITRKAYGGAYDVMNSKHIGADMNYAWPSAEIAVMGAKGAAEIIFK
                     NEIAKAENPEAKLQEKVDEYTEKFANPYKAAARGYIDEVILPEDTRAKLIRAFEMLEN
                     KHIDQPKRKHGNIPL"
     gene            18120..18521
                     /locus_tag="PLS16_03885"
     CDS             18120..18521
                     /locus_tag="PLS16_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60254.1"
                     /translation="MKKILYFFVATLFFVACKQGHTHNEVIQLDNGKKWQVSEGMKPF
                     IKNGADLLDAYVVSKDEDYKKLAEKIRNENNQLINNCTMKGKSHDELHKWLHPHLELV
                     DALGKASDTTEANKIIQLLQQSNLTYQQYFE"
     gene            18602..19285
                     /locus_tag="PLS16_03890"
     CDS             18602..19285
                     /locus_tag="PLS16_03890"
                     /inference="COORDINATES: protein motif:HMM:NF013428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNJ60255.1"
                     /translation="MQLVYKGKTKDVYSTEDANQYLLKFKDDVTGTDGVFDPGANTVG
                     LSIEGAGKSGLKMTTYFYKLLNTKGIATHFVDSDIENVTMTVKKGTVFGKGLEVICRF
                     KAVGSFFRRYGDYCKAAQDLGVFVEFTIKDDERGDPTISKDALEILGILNANEYEVIK
                     ENTIHICTIIKDELAAKNLDLYDIKLEFGRDIQGNVMLIDEISGGNMRVYQNGKYLEP
                     LQLEQVFLA"
     gene            complement(19289..19861)
                     /locus_tag="PLS16_03895"
     CDS             complement(19289..19861)
                     /locus_tag="PLS16_03895"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HNJ60256.1"
                     /translation="MNDILTLEPLYLAPISFYQNIIHQNITLTTNSIYTKKTLKNRTY
                     ILGANGVLRLSIPLQHQQNERRKYADVKISYAEKWQKDHWNSIVSAYRRSPYFEYYEE
                     ELKYFYDNQFDSLSTFNIELHQWILKKLQIEKAIVYNNEVSKIQVEKTYEPITYLQVF
                     SDRFEFQPNLSIIDALFNLGGKGLFKLLSR"
     gene            complement(19930..20916)
                     /gene="rluF"
                     /locus_tag="PLS16_03900"
     CDS             complement(19930..20916)
                     /gene="rluF"
                     /locus_tag="PLS16_03900"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: protein motif:HMM:NF007784.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HNJ60257.1"
                     /translation="MQKGSGVWLNKFIGESGLCSRREADKLIERGVVFINKKKAQKGD
                     QVFAGDIVKVNGQELEPLEKEDVIFIALNKPVGIVSTTEKGVKNNIVDFVNYGVRIFP
                     IGRLDKDSQGLIFLTNDGDMVNRILRAGNNHEKEYVVTVNKPLTDAAINGMAQGVPML
                     GITTKKCRIIKESTKTFRIFLVQGLNRQIRRMCEFYNYEVTKLERVRIMNITLKGLPE
                     GEWRILEEDEMNKIYQLTESSISEEKKAVIPAKSASSNNTTNKTERNKNTIKTGNSTK
                     SQKGTHKKLASKNEKSVNSRNSSKTNTISRTAKPKSSNTKSAQNTSGRKTKR"
     gene            21218..21661
                     /locus_tag="PLS16_03905"
     CDS             21218..21661
                     /locus_tag="PLS16_03905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNJ60258.1"
                     /translation="MFSKACEYGIRATVYIADQSKQSNRAILKDIAKEIDSPEAFTAK
                     ILQQLVKSGIVHSVKGINGGFEISKDKISNLKLADVVLAIDGEGIYTNCAFGLKNCSE
                     QFPCPVHHKFKPIKANIRKMLEGTSINDMSDKLKDGLAFLKLENY"
     gene            21678..>23084
                     /locus_tag="PLS16_03910"
     CDS             21678..>23084
                     /locus_tag="PLS16_03910"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNJ60259.1"
                     /translation="MNGGQIIAKVLQNHGVKYLFTLCGGHISPIFVSAEQLGIRVIDT
                     RHEANAVFAADAVSRLTGIPGVATVTAGPGLTNTITAVKNAYLAQSPLVLLGGAAATI
                     LKGRGSLQDINQMSIMRPNVKWAATISKVKDIVPTLEKAFEVAQRGVPGPVFVECPID
                     ILYGEELVREWYGAKSKDVPPKNIQERAIQWYINWHAQRIFEDKDKVTFGQPRPVYFP
                     NHNSSDINSAVDYLKKAKNPLLVLGSGAMMQPQKAQQLADAVNKLGIPTYLSGMGRGL
                     LGKDGVLQMRHKRKEAIKDADLIILAGVPNDFRLDYGNHIGHRPFISINRSTEDLNKN
                     KKPNLGINADPQDFLIELANKYQLKNNSAWLSKLKDRDNAREKNIDEQAAGKVNGINP
                     VQLFREMDPMLSDNTILIADGGDFVGTAAYTLKARSPLSWLDPGVFGTLGVGAGFALG
                     AKLVYPEKEVIVEDHIYRL"
BASE COUNT         7652 a         3672 c         3631 g         8129 t
ORIGIN      
        1 ctacttacta caacaacaaa gtcttgattc ttggctctaa aaatcttgat tctcatctct
       61 cactactcaa tactttctac ttaatactac atgcacatct cttataaaaa tgaaaacaaa
      121 ttggtatctt tgtaaaaaaa atcattgaaa agtcctattt attttgataa tgctgcgaca
      181 actgctatag cacctgaagt tgtacaggca atgttgcctt ttttgaatga gcattatgga
      241 aatccatcat ctgtttatag tttggggcga gatactagag ctgcgattga gactgcaaga
      301 aaaacgatag cacaaatttt aaatgcccaa acttctgaaa ttgtatttac atcttgtggt
      361 actgaagcca ataatatggc aataaaaggt gcagttattt atcatcaagt taaaaggatt
      421 atcacttctc caatagaaca tcattgtgtg attcatattg cagattattt aaaggaattc
      481 catcaagtaa aaattgagta tgtgcaatta ttatccgatg gtatggtgga tttggaacat
      541 ttagaaagtt tattaaaaga caatcaggat aaaactttag tatcattgat gtatgcaaat
      601 aatgaaattg gtaatctttt agattttgaa aaagtaagcc aactttgtaa aacacatcag
      661 gctttatttc attctgatac cgtgcaggca tttggacatt ttcctataga tgttcagaaa
      721 gtcgaggtgg attttctttc tgcttcagca cataaatttc atggaccaaa aggtattggc
      781 tttttgtatc aaaagaaaaa aagtaaagtg gcttctttct tacatggagg tgggcaggag
      841 cgtggctatc gagctggaac tgaaaatgtt tatggcatag ttggtttggg caaagctgct
      901 gagttggctt atcagcattt ggagaaagat agacaatata tccaatctat taaggattat
      961 tttaaagaac agctattagc taattttaag gatattcaat ttaatggcca ttccaaaaat
     1021 agtttattta cggtcttaaa tgtatctttt ccagaaaatg atgccgctag tatgttgttg
     1081 tttaatttag atattgaagg tatctgtgcg agtggtggta gtgcttgtaa tagtggagct
     1141 tcaacaggtt cgcatgtgat tgatgccatt cgaccgaata gcaatagaat caatattcgt
     1201 ttttcttttt cgaaatataa tcatctatcg gaagtggatg tggtaatcca agtactaaaa
     1261 aaatattttt cataaatacc taaatttatt tatttggatt ttgtagctgg agaaataata
     1321 ttgattgata agccattgga ttggacatct tttgatgtgg taaataagtt gaaatttaaa
     1381 cttaaatatc aatacggaaa aataaaaatt ggacatgcag gcactttaga tccaagagca
     1441 agtggtttgt taattgtgtg tactggaaaa aaaacaaaag aaatagaagg tattcaaaat
     1501 caaaataaag tttataccgg gagtttttat ttaggtgctg aaacagaaag ttacgataca
     1561 gaaaaaccag ctatcaattt tcaaagtatt gagcatatta cagaacaaga aattcataag
     1621 actgcggaaa gttttttagg agaacaacaa caatttccac ctatccattc tgctgttaag
     1681 atagatggtg ttagagctta tgaactagcc agaattggct taaatcctga aatcaaatct
     1741 aaagtcattc aaatttatca atttaaaatt acaaaaatag aattgcctta tatctatttt
     1801 tatatcgaat gtagtaaagg tacttatatt cgtagtattg ctaatgattt tggtaaaagg
     1861 cttaacaatg ttgcctattt agcaagtttg aggagagaaa aaattggtaa tttttctatt
     1921 gaagaagcat atacagtgga ttcgtttata caaaaattag atgctattca atagctctat
     1981 tgttttgctt ctttgaactt tttcagattc agttctaata aattttggaa ggaaaaatat
     2041 agttttgggt cgttcatatt tttctaaata ttgttccaaa tattgttcta aatgatgaag
     2101 tttttcgcta gtatattttt catcttcaat caatagacaa agtttctctc caagtgtatc
     2161 atctggtaaa cctgcaataa aataagcagt tttgatataa tcggatagtt tagtttcaat
     2221 cgtttctgga aataatttta taccaccact attgattata ttatccaatc ttcctttcca
     2281 aataaaagta gtttcgttta tcaaatcgac aatatcatta gtataaattt tttcagttaa
     2341 aaagggaact tctaattgta aacactgccg attgtctatg ctacaaagca cacctggaag
     2401 agcttgataa gccaaggatg gttttgaccc attaattttt cttaatgcta catgacttag
     2461 agtttctgtc ataccataac catgataaat ttcagcagat aaagtttggc aagcttgttc
     2521 taattcagca ctaacaggac caccacctaa taaaattgtt ttgatatttg aggtctgttt
     2581 tgtgcctttt aattgcatag gtgtgagcgg tacaaaatct attatagtat tggctataaa
     2641 atgatttaaa ggttggctat ttggttcaat acatattaaa ttcaattgac tatataaagc
     2701 acgaaccaac atcattttac cagcgatgta attcattggt aagcacaata aagcagtttg
     2761 attttctttg aaattaaaaa aatgggctgt tgcttttgca ctttgcaaca tttgttgttt
     2821 ggagacagta attgttttgg gtaagcttgt agaaccgcta gtttgaactt ctattgttgt
     2881 ttttggagaa aaccattcaa ccataaaatc gccaatttta ttaatatttt cgatattact
     2941 tttcttgcaa aattctataa tttcttcatc tactaagcga tgcccattta ttgtcaattg
     3001 cttgtattct aaatgattca tattagtgca gataaatccc atttttggtt tttatggtaa
     3061 tgtaatccat tggcatcaat actataagga gctgggaaat tattcgtaaa taattgtcca
     3121 gttcctaatc cttgagcgta atccgagcat tttaaagctg tgtattgtgc gatggcatta
     3181 agcccaatat tactttctaa agcggaagtg ataacccaag taccaccatt gttttcaatt
     3241 aattttttcc attcatcggc agcataaaat ccgccaataa gtgcatgttt taaaattaaa
     3301 atttgtggtt gaatcgtgtc aattaatgcc tttcttttac ttgaattttg tattccgata
     3361 agttcttcat ctaatgcaat ttttataggt gaattattgc ataaagcagc catatcttgc
     3421 cattgcccag tattgatagg ttgttcaata taactaattc caaaatcgga cagttgtttt
     3481 attttttctt tagcttcttg gtagataaat gcaccatttg catctaatcg aatttctact
     3541 tcatcaaccg aaaattgttt tctgatgtat tgtaataatt ctagttctgt atcaaaatcg
     3601 atagctccaa tttttaattt gatagattta taatttgcat ctaattttaa ttttatttgt
     3661 tgcaacatac tttctttgct acccatccaa atcaatccat ttgttggaat tgtgaagcca
     3721 ttttcattga ttgcttctgg aaatatgatt ctgttgccgc catttcccca atcttttaaa
     3781 agcatttcaa taccgaaaaa tatagaaggc cattcggtta gttcttgcaa taccgcttcc
     3841 atttcatcag gtaagcgact gcaaattttt gctagttttg cttcataatt tggtctgtca
     3901 tcataggaaa gtttggtaaa tcggttacat tctccaatag cagttttttc ttcattttgt
     3961 aagaagataa aataagtttc tttttcatac aaaacgccac gagatgtacc accaggttgt
     4021 ttaaacttta gagcatattt cttaaaatag cagttcacac tacaaatcta ctgattttta
     4081 aactatcaaa cctaatccaa ataaaataga aaaaagtaag gtgctgatgg ccaatttttt
     4141 taattcaggg tctaataatt ctggcgtttt attattcaaa acagttgtta tgcttttgat
     4201 tatgcctata aatgctatta aataaatcca attccagaga gatgctccag ctaaaaaagt
     4261 gtaaataatt gcagtgagca taccaatgcc taataaaatg gcatgataga tttttgcttt
     4321 ttcaacgcct aattttacaa ccattgttct tttgccaaat gctttgtcat ttattctatc
     4381 tcttaaatta ttcaaattta aaacgcctgt actaaagcaa ccaatactta tcgctggtaa
     4441 aaattctaat cctgatattt cgtgcgtatg tagaaaaaat gttcccatta cgcccacaat
     4501 tccaaagaaa ataaagacaa aaaaatcgcc caaaccacta tatccatagg gatttttgcc
     4561 catagtatat ttcatggcgg cagcaatagc agcaattcct aataaaagat aaattagacc
     4621 ataagaaaat tccatacctc gagttccaac tacaattaaa gcaattccag atacaaatga
     4681 aagcaccaca aaagtaacta tagcgagttt catttgcttt aaggttattg ctcctgattg
     4741 taaacttcgt tgaggtccaa ctctttcttg attatccacg cctgatatag tatcgccgta
     4801 atcattggct aaattgctta aaatttgaag aaataatgtt gtaattactg ctaaaataaa
     4861 tacactccat tgaaaactat ttctgtaagt agctaaaaaa cttcccagaa taatggttga
     4921 taatgaaagt ggtaatgttc gcagtctaaa acttactaac caagcagaaa ttgatgccat
     4981 tttaattatg gaaatttagg aaatttatta aaattaggtg ctcttttttc taagaatgca
     5041 tttctgcctt cttcagcttc ttcggtggtg tagattaatc ttgtagcttc gcctgcatag
     5101 acttgttgcc ctactaaacc atcatctatt aaattaaatc caaatttagc catttttata
     5161 gctgttggac ttttagtaag aacttcttga gcccattcaa aagcagtatc ttctaattcc
     5221 tcgtgtggga caacagcatt gaccataccc atttcataag cttcttgtgc agaataggag
     5281 cgacctaaaa agaaaatttc tcttgctctt ttttgcccta ccattcttgc taaataagca
     5341 gaaccataac ccccatcaac actagcaaca ttcacatctg tttgtttaaa gatagcgtgt
     5401 tctttacttg ctaaagttaa atcacaaaca acatgcaagc tatgaccacc accaactgcc
     5461 caaccaggca cgacagcaat caccacttta ttcataaatc ggatttgtct ttgtacatct
     5521 aaaatattaa aacggtttat gccatcgcca cctttataac ctgtttttga acgtacacgc
     5581 tgatcgccac cagagcaaaa agcccaaccg ccatctttgg gagaaggccc ttcgccacta
     5641 atcaatacca ctccaatttc ttgcgattca tgacaaatat ccaaggcttc ttgaatagct
     5701 gaaactgttt ctggtcgaaa tgcatttctt acttctggac gattaaaagc tattcttgcg
     5761 acaccatcac aatatttaaa agtgatatct tcatattctt taatggtttg ccattttatt
     5821 ttactcataa gtctatttta tatcttttag taatttccaa tattgttcta agataatagg
     5881 attttgttca gcatcagtaa aaatctctaa tattgttcgt tttgtagttt tattgaaaaa
     5941 ttgtattagt gtagattcta atgtattttc ctcttttaca gatagataat tccattgata
     6001 atgttctgct aatttttcag cattagtatg cattgatgtt tcaatgaatt gttctcgttc
     6061 tgcaatttta tcagggccag gaataattct aaaaatccca ccaccactat tgttgatgat
     6121 aattactttt agattatcta tttgttcatc aatccaaaaa gcatttttat cataatgaaa
     6181 tgaaatatcg ccactaatta ataaaaaatc tttgtcaggt gcagatgcag cagagccaac
     6241 gaccgtactg ctactaccat caataccact tgtacctcga ttgctccatg tgcatttaat
     6301 ttttgaattg tcaaacagtt gagcataacg aatagcagaa ctattggcta tatgaacaga
     6361 aattgtagga gttattgttt tataaattgc attaaaaaca ctgaaatcac tataaggaat
     6421 ttgcttgcaa aaagcttgat gtaattgttc tttttgattt tttaaggcta tccaagttga
     6481 tttataagtt gatgcaattg gttttatctg ctctaataat tgctgaagaa atattgttgg
     6541 ctgtaaggca attgcatctg ttaaagattg ataagtatcc atcattgaat caaactcatc
     6601 tatgttccaa tgaaatgtag gtgcgtaatc acgtaatagt ttttttattc ttttagaaac
     6661 aattgcaccg ccaatgctta ttaataaatc gggcattaat ttttgagtgt cttcagcagt
     6721 taagcctgtg atacatctat ctatgttttc gacaaaatca ggatgatgta gatttgaagt
     6781 tgattctgta agaataataa cattttcgag ttgagcaatt ttggataata ggtattgtag
     6841 tttttgattg gggtataatt gtccaactaa aatcaatact tttttactac ttgaaaattg
     6901 attagccaac tgctctaaat aagttttaga aagttgtttt tctaaaggaa ttgctttgaa
     6961 aattttcggt aaattatttg tatctattga gcttgtacca taaagtggtt cactcacagg
     7021 aatattaaaa tgaactggac ctttgtctat taaagtggcg atattaaagc cttcacttat
     7081 acaacgttcg atataccata aatcattttt ttgaacagca tctccattga tttcatagct
     7141 tttacgaata taattagtgt aaatattagt ttgtaaaatt gtttgtccgt cgccttgatt
     7201 tgtccattct tttgggcgat cggttgtgag cacaattaat ggaatccttt ggtaataagc
     7261 ttcaactata gctggagcaa aatttaatgc agcagaacca ctagtacaaa gaatcgcaac
     7321 tggttcttga agctctatgg ctttacctaa tgcgtaaaat cctgcagttc tctcgtctct
     7381 aatacttgta caattaaaag caggatgtcg attgaatgaa attacaaaag aggcatttct
     7441 tgaacctggg catattacaa tttcttttaa acccaaagcc gctaaagact gtacaatttg
     7501 ttgcactcct ttcttatccg acaattccat tttctaattt tatagtgttc gattgtagtt
     7561 tctcagacat agttttgctt tttaagatgg tttctttcca ttcatcatca ggattggatg
     7621 ctgctgtaat accaccacca acatagatgg ctaaatggtc tttccctact tgcaaacatc
     7681 gtaaattgat atataaattt gtagtttccg aaaaattagt ttctccaata aaaccacaat
     7741 aatattttcg attatagcct tcatgttgaa gaatacaatc gatgccattt tttacgggca
     7801 aaccgccgat tgaaggtgtt ggatgcaaat ccttgagtaa ggaagctaat tcaatttttt
     7861 ttgtttcttc aaatatataa tcagttttta gatgagctac ttgtgcagat tctactgttt
     7921 gaagtgcctt tttatctata acagcacaag catatttttt aagaacttgt tcaatatgtt
     7981 gattgaccat ttgatgttct tcaatttctt tagaatgcca tttgtattct aaacctactt
     8041 tatgacgagc ttgagtgcca gccaaagcca taatgtaaaa tttgttatct tgctttttga
     8101 taagtaattc aggtgttgct cctatccaaa ttcctgatgt tggatgttga gaaaaatgaa
     8161 cgaaagtatc tgggtaatct tttgctaatt ttaagaaaaa atcaatagga ttgaaatctt
     8221 gtggcttatc ttcaagaata acacgtgata aaactgcttt gtccaaattg cctttatgaa
     8281 tctcctctaa aaaaagttga attctatgga aatattgagt ttttgtagtt gcatttggta
     8341 agttgctttc tatttctttt tgctgaggta aacttttgac aaaatttaaa aaagattcat
     8401 ttatttcatt gtcattcaat acatcaaaaa aaatatcttc ataagaatta tttgttatga
     8461 atggagcaaa ccagaatgtt tttctattat ttgattcaag caatgattta tcaagggcaa
     8521 tacttattct ttgttcgttt gggaaacgat aaatacaaaa aggaatattt ttagaaaata
     8581 atatccttgc tattgatatg acgaaacgct ccaagacaaa tctttagaaa ggcaaattta
     8641 gcaaaaaata aggagcttta aaactatgta ataatttagc aatgtggctt agtctaattt
     8701 ggtatatgaa aatctatctt ttaacaaaag agactttgat tcaaacgagt tctaagttta
     8761 aatcaactat tttatctgaa taaaaaaatt attgataaat cttatttctc aataaatgat
     8821 cgacaataac taaagctgcc atagcttcaa cgataggcac tgctctaggt aaaacacatg
     8881 ggtcgtgtct gcccttgcct gaaacaatag cttcatttcc ttcggtatca atagaaactt
     8941 gatctcgcat aatggttgcc actggtttaa atgcagcgtt aaaataaata tccattccat
     9001 tactgatgcc accttggata ccaccagaat aatttgtttt ggtttttaca gtatcttctt
     9061 ctgtataaaa taggtcattg tgttggctac cttttaaatc caaaccagca aatccacttc
     9121 catattcaaa ccctttacac gcattgatag agagcattgc ctttcctaaa tctgcatgta
     9181 gtttatcaaa aactggttcg cctaaaccaa caggaacacc ctgaataaca caagaaacaa
     9241 tgccaccaat cgtatcgccc tcttttctga tttgtttgat gtattcaatc atttgttccg
     9301 ccatattatt gtcaggacaa cgaacaatgt tattttctgt attgcttaaa tctaattcgg
     9361 tatagttttg tgctaattgc aaatgtccaa ctctagaaac ataagcttga atttggatac
     9421 catattgctt taaaatcaac ttagcaattc cagcagcaac tactctcgca gctgtttctc
     9481 ttgctgatga acgtccacca ccacgataat ctcgagtgcc atatttttct tgataggtaa
     9541 aatcggcatg tgatggtcga aaggcatttt taatatggtc gtaatcttta gatttttggt
     9601 cttcatttct gatgataata ccgattggtg taccctgagt tttgccttca aaaacaccag
     9661 ataatagctc aaattcatca gcttcttttc tttgagtaac aattgcagat tgcccaggtt
     9721 tacgacggtc taattcgttt tgtaaaaagg cagtgtccat ttcaattcct gcaggacagc
     9781 catcaatcac tacacctaaa gcgacaccat gactttctcc aaaagtcgtt attctaaata
     9841 tttctccaaa tgaatttcca gccataatgt tattttataa aataaaaata gctttcatta
     9901 aaaattaatg gaagctattt ttattattat ttagaggagt gattagtgtt tgttagacaa
     9961 cactttatca ttcacaaata gtattccaaa aactaaagtt atgaacccta taaggaaagg
    10021 ataaaccatg tgaacatcta ttatagaagg ttgtttcgct acattataat tcaaatgtgc
    10081 agatgtattc aaatacacaa agaatgagta taataaaata actgaaccaa caaatgaaag
    10141 aatcgcccct agaacttttg taagctttgc cataaaaaat attttttcca aaggtattaa
    10201 aaatataaga aaattacaaa aaaaagtatt ccacaacaat aaattaatct ttactgacaa
    10261 ttttaatttg ataggcaagt atccaacata taactccagc aataacggat atataaccaa
    10321 ctctatcata atgatgcacc cagcctgtat ttttatcaat ggtaaccaca aaactactgc
    10381 aaacagaagc gataaaagta cctaattcca aaaaggcgga acgcatactc ataaaagcac
    10441 ctcttttttc ctttaccatt gctgcagaca ttatggtata agctggaatc atacgaccag
    10501 ccgcaaatat gaagaaaaat acacagataa taataacttc taagatattg gcttttgcaa
    10561 tgtgtgtagt aacgataatt gggaaaaaag atagaaatat taatatcgaa aatgaaggtt
    10621 tgttgccgta tttatcggat agttttccaa taataattga actaattacc gtaacaattc
    10681 cgccaattaa gtatagaata ggtatccaat cttcgccaaa accaatgttg ctttcaaaat
    10741 aaggtactac aaaaatgacc acaacttgat gactgaatac taagaaaaaa gctaaaagta
    10801 aagctttaac ttggttgctg tctttgaaaa catctttaat atttgaatag cttatttttt
    10861 ttgtattatc ttggtttata tgtcctttca ttggtggaat ccacagaata atggcaaata
    10921 ttgtaaaaat agaaataata gttagccaca aaaatggggt aaacaacgta taatgttggg
    10981 ctaaatatat accgaaagga atacctgtta tggctgcaat tgcaaaagac aaagaaataa
    11041 tactaatagc agttgttctt ctttcataag gaattatatc actaacaatt gcaatcgcaa
    11101 tgcctccaat aataccacca aatacaccag taaatgctcg ataggcaatc attaatccaa
    11161 aatgtgaatg catagaacat aagccagttc caattgtaaa tccaatgaaa gtaatcagta
    11221 ataatttttt cctatcatat aaatccataa atgaagagga aaatattcct gaaataaaag
    11281 ctgcaaatga ataaatggat acaataattg ttgctgtctt tgctccaatt ttgtaagtat
    11341 ccattacaat atttatcata ggcatcaata gcatagaatc tacggtacat aaataagtaa
    11401 taaatgctaa taaaataact attaaaaatt cgttttgtct gccgtgtggt aaatcttttt
    11461 cttcaagaat taaactatct aaactatcca tggtgcaaaa tctgaaaaat aattcaataa
    11521 aagctaacca taattgatta aatgttaaat tctagaaatg ccatctgaaa tatgacaagg
    11581 ataaaattct gctttgagtt tggtttttct ttcgtaattg acacttatgt attctataaa
    11641 tttatctaat gaattttttt caactaatgc aattacacaa ccaccaaatc caccacctat
    11701 cattctggca ccaatgcatc ctgaaaaatg tccaataata tgaattaaag catctatttc
    11761 ttcacaagaa acctcataat ctttactcaa tgaatgatgt gattgcttta ataaatcgcc
    11821 aaaaatttca tgttcatttt gtgctagtgc gtctgttgct aataatactc tattattttc
    11881 agcaacagca tgttttgctc gcttgtataa aatatcatca tcaagataag ctaatgaaat
    11941 accatgcacc tgacataaat tctcagcaac atcaaattgt tttaaaatct gaaatgctgc
    12001 atcacactga ctgcgacgct cattgtattt agagtccgct aaagttcttg gtgtattaga
    12061 atttataatg actaattggt aatcaccaaa atctaaagga atatgttcaa aatctaaagt
    12121 atcgcaattc aataaaattg cgttattttt tttgccattt gcaacagcaa attgatccat
    12181 aattccacaa tttacaccaa catatttaac ctcagcattt tgtgcatcta aagctaattc
    12241 agaacgattt ttattgtaat atatttcatt aaaaatataa gcaattaaac attctaaagc
    12301 agcagaagag gagagtcctg tacctaatgg caaatcacta tcaattaaaa tatctactcc
    12361 caaaagaggt gtatttttgt ctagtaaaac ttgtaatgtt gatgtaatat atcttgccca
    12421 acctatattt ttatttaagt tgagcggttt ttgaaggtca atactaattg cttcttcaaa
    12481 gttttgagca tatacattta taatattatc ctttcttctt ccaacgatgg ccgtaattcc
    12541 taaagaaatt gcagcgggta aaacatagcc accactataa tcgatatgct ctcctattaa
    12601 atttattctt ccaggcgaga aaaaagtatc agttgcggta taaccaaaat aggtatggaa
    12661 atttgctttc agtgagttgg tatctaacat atattatttt aaaaatctta gtgggttaaa
    12721 agtatgaata atatttttta ttttatctcg gacattttct ttcatataat ttccaaaaag
    12781 tttagtcatg tgttgtgtca attctttatt gtgctgattt cctgccgata aaatcatatc
    12841 tttcatttcc tttttactta aatcttgcca tttgtatgga atttcttcca ttaattcata
    12901 accttcaata agttttcctg catcatacat ttttgataaa gttccgttat catatttcat
    12961 aaaaaaaatg ggatctactt tgtattttaa aagcctatct tttgttccag gttttaaata
    13021 atagtgtctt aattcagcat ttttttgtgt aaaacttaat ccaatggcta atcgtgcatt
    13081 atttgtaata ttgggtggcg aagcgtggaa tgttctattg tcaaaaatga gtgcttcgcc
    13141 cgctttcata ggtaataatt ggaaatatgg gaacatcgaa aacatgtgtt ttgccaacgg
    13201 agacggtact tgtggcgaag gtgaagggcg tacactattg aaaaatttat tactaccttt
    13261 aattacgcct aaacaaccgt tttccatatc tgtatctacc aatgcaatcc agcaggttac
    13321 cgaacaatgg cggttttcat cttccacaaa actccaatct tgatgtggag gtactacgcc
    13381 ttttggattg gggtctttca aaacataact tgctgtaatt aattgataat tacataaata
    13441 aggagcgatt tttggcattg caaccgcttc aattgtatgc atcatttttg caaccaaagt
    13501 tttgtcttca tgatccatac caacataaaa accaaaatca ttttcttttt taatacccga
    13561 attgtataaa aaatcgatta atgtctttat ctcagtttgc tctaacgcag gtaattttat
    13621 atagccgttt tgttcaaaaa attgttgatg agcttcgtta ctaaataatg gttccataat
    13681 tgaatatttt tggcagtgag cgtttagatt ttgaataaat tgtttttcac ttaattgagg
    13741 aacttggtaa gaaaatgttt tttccaattg accaattaat ggctgttgaa taatttcagg
    13801 ataatgcaga aataaatcat caggcatact gtattgttga atttctccct tgtttttatc
    13861 aaaatacaac ataaacaaat ttgcctcttt tggaactaat ccaatggtta atgccaaccg
    13921 ttcttcatta ctttgattgg gccacgaagc atgcattaat ttctgattaa agataaaagc
    13981 ttcgcctgtt tttagtgtta aagtttttaa atatttatcg aatgcaggtc tttctttttc
    14041 aaatgaaact ggcaaactag gtgctcgcat cgaatcatct atttgatggc ttctatcaat
    14101 aactcttatt gcaccatttg tttggttggt atcttgtaat ggaatccaaa tagtgtatga
    14161 accatacttt ttttcatcgg ttactgtcca atcttgatgt agtggtaaag gattattttg
    14221 attcggtaat tttcttaaaa aggaagaacc taaaatttta taatcagcaa agtttttatc
    14281 taaaacaggt tgtattatat ttaatatttc ttgatttact ttttgtttta attcaaagtc
    14341 tggatagaat gaagtggaat aaaaagtggt cgttttatca tcaaaatttg caaagtaaat
    14401 agacttaatt ttttctgcta aatcattatc caatatttgt tgggtgaaat agccatttat
    14461 ctccaataat ttttggtgat atgcatcttt aacatttaac attagttgaa tacagctttt
    14521 attttttcaa aaaaagatgg cttttcttca attactggag tttcatcaat tcttgtatta
    14581 aacaaaccct tttcgtattc tttcaaactc aaattgaatg gcttgtaatt gaaagttcta
    14641 agtacttttg cattttcagg ttgtttccaa ggattaaatt ccatataaaa atcaacatca
    14701 acttccaaaa catcaacttt gcttgagctt ttttgtggat tcatgtggta atgtattgat
    14761 ttttcttctg ctggaattaa aattaattga attgccaaac gcaaaccatc tgtttgattg
    14821 atattagaat aatgtactaa actatcatct aataccacag cttgtccagc ttttagattc
    14881 attggaatca tgtgtttgtt aatgatgtca tgtttgatgt tttctaattc ccatggaatc
    14941 attggacctc taacttctcc aaatcttttg tgcgaaccag gtgtaacttc caaagtacca
    15001 ttttgactat tgctatccac taatggacac caaattgaaa cgctggtaac ttttctttca
    15061 tcaacaaaag cccaattttg atgcaaaggt acttcgccat cattggattg ttttctaata
    15121 aaattggcaa ttacaggacg ataattatct aaatatttat cgtaatgtgg tttgaatttt
    15181 tctgaaataa ggttgtacac cattctttta taatcaatat ttttattgat aaatgtaaag
    15241 tcataagtaa ttctttcttt caggttggca tcttgttctc caatattacc accactttgt
    15301 tctaaagttt caaagtattt atttttaaga aattcaactt cttcttcatt taagaaagga
    15361 atgatgacgt agccatcttt ttgaaattgt gattctaatg cagcgtcttt aaatacattt
    15421 ctcataacta attattttgt ataaaaatgt ttattttcgg ctacaaaata cgaatattat
    15481 gtggccgttt agtaaaagta aaaaaaataa aaaaaatact gctgatcatg caatagatgt
    15541 gggaaatttt tatgataaga atcatagtgc ttttattcag gtttatggcg aagtaattca
    15601 agcatttaga acaaataatt tagattcgat tttgaactat cagatacatc agattggttt
    15661 aaaacctgaa tacaaagtct tagatgcagg ttgtggtgta tgtggtccag cacgatattt
    15721 tgctaaaaat actggctgct ccattgatgc agtatctgtt tctgcaacgc aagtaaatag
    15781 tgcgaaagcc tatatagaaa aagaaaattt acaagataaa atttcagttc atcagtatga
    15841 ttatcatgaa gttgataagt tattcccaaa agaaaatttt gataaaatat attttttaga
    15901 atcttttgga cattcaacag ataaagaaaa attgctgaaa gctatgtgga aagtattaaa
    15961 gcctggaggg gaaatttata tcaaagactt atttcgtaga atttcgagca gttggttggt
    16021 gcaacgaaaa attgatattg aaattaataa aatcaataaa gcataccatt atgaagtggc
    16081 tgatttaagt gagtttgtca atgttgttcg tgaaattggt tatgttattg agtttgtaaa
    16141 aacgatagac ataccattag atgaatttga aaatttatct atttctaatg aatttcaaga
    16201 attgacagga attgccaaaa ttgataattg ggaaaaatat atttttccta tagatttctt
    16261 tgaagtgaaa ttatataaac cagagtatga tattgaaaaa ggtaaaggaa gatatttctt
    16321 acaaaatatc tatcaaatac aaatgaatca tttgaataag gaacatttgt aattacaaag
    16381 gaatattgcc atgtttacgt ttaggttggt caatgtgttt attttccaac atctcaaaag
    16441 cacgaattaa tttagcacga gtgtcttcag gcaaaatcac ttcatcaata taaccacgag
    16501 ctgcagcttt atatggattg gcaaattttt cagtgtattc atctactttt tcttgcaatt
    16561 ttgcttcagg attttcagct ttagcaatct catttttaaa gataatttct gctgcacctt
    16621 ttgcacccat cacggcaatt tctgcactag gccatgcata attcatatcg gcaccaatat
    16681 gtttggagtt cattacatca taagctccac catacgcttt tcttgtaata acagtaactt
    16741 ttggaacggt agcttcgctt aatgcaaata ataattttgc tccatttaca ataattccat
    16801 tccattcttg gtcagtacct ggtaaaaatc ctggcacatc taccaaaact aataatggaa
    16861 tattgaaagc atcacaaaaa cgagtaaatc tggcaccttt tcgtgagcta ttgatatcca
    16921 aaacacctgc taaataagct ggttgattgg caacaatacc aatacttcta ccagcaattc
    16981 gggcaaaacc caccacaata ttttctgcaa aacctttatg tacttcaaaa aaactatcct
    17041 catcaactga gagtgcaata acatctctca tatcataagg ttgatttgga ttttcaggca
    17101 gtatattttt taaagcttca attttttcag aaactttttg tgcgtaatct acttttggtg
    17161 ttttttcttg attgttttga ggcaaatagc ttagtaattg tttgagctgc ataatacatt
    17221 ccacttcatt tttagcagta aaatgtgtta cgccagattt tgtagcatgt gtcattgcac
    17281 cacctaattc ttcgctgctt acaatttcgt gcgtaaccgt ttttacgaca ttgggtccag
    17341 ttacaaacat ataagaagta tgctcaacca tcattgtaaa atcagtcatt gcaggagaat
    17401 aaacagcacc accagcacaa ggtcccataa tggctgaaat ttgaggaatt acacctgaag
    17461 attgcacatt tctatggaaa atatcggcat aaccacccaa tgaaacaaca ccttcttgga
    17521 ttcttgcacc accagagtca tttaaaccaa ttactggagc tccaacttta gttgctaaat
    17581 ccattatttt acaaattttc tcagcatacg tttctgaaag tgagcctcca aaaacagtaa
    17641 aatcttgtga gaatatatat actaatcggt tgtttacttt gccatatcct gtaattacgc
    17701 catcgcctaa atattgttcc ttatccaatc caaaatcggt gcttcggtga acgacaaatt
    17761 tgtctaattc ttcaaaagta cctttatcaa ccaataattc tattctttca cgagcagtta
    17821 atttgcctct tttatgttga gcatcgatac gcttttgacc gccacccaat aaagcttctt
    17881 gctctttttg atataactct tctaactttt gattgattga actcatgtgg ctaagatagt
    17941 tcatttattt tatttttgaa tatggtgcaa tgtctatttc aataaaataa tccaaaggta
    18001 atttttaaat tcaaatgtta attattcttt tttctatgca ttattactag gtatttatac
    18061 ttctatattt taacgtttct aatttaacaa gtagatttga catgaattaa tttatagata
    18121 tgaaaaaaat attatacttt tttgttgcca ctttattttt tgttgcttgt aaacaaggac
    18181 atacccataa cgaagttatt caactcgata atgggaaaaa atggcaagtg agtgaaggga
    18241 tgaagccttt tattaaaaac ggagcagatt tgctcgatgc ttatgttgtt tctaaagatg
    18301 aggattacaa aaaattagct gaaaagatta gaaatgagaa caatcagttg attaataact
    18361 gtacgatgaa aggtaaaagc catgatgaat tgcataaatg gttacatcct catttggaat
    18421 tagtagatgc gttgggaaaa gctagcgata caactgaggc aaataaaatt attcaactat
    18481 tgcaacaatc taatttaact tatcagcaat attttgagta aataaaatgt agattttact
    18541 ttaaaatgat gctgttttat ttattttatg atatttttga gccactaaaa ttttagaatt
    18601 tatgcaatta gtttataaag gaaaaactaa agatgtttac tcaacagaag atgcaaatca
    18661 atatttattg aaatttaaag atgatgtaac tggtacagat ggcgtttttg atccaggagc
    18721 aaacacggtt ggcttgtcta ttgaaggtgc cggaaagtct ggtttgaaaa tgacaaccta
    18781 tttttataag cttttaaata caaaaggaat agctacacat tttgttgatt cagatattga
    18841 aaatgtaacg atgacagtaa aaaaaggtac tgtttttgga aaaggattag aagttatttg
    18901 tcgttttaaa gcagtaggaa gtttttttag aagatacggt gattattgta aagcagcaca
    18961 agatttagga gtttttgtcg aatttacaat taaagatgat gaaagaggcg atccaactat
    19021 ttctaaagat gccttagaaa tattaggtat tttgaacgca aatgaatacg aggtaatcaa
    19081 agaaaatact attcatattt gtactatcat taaagatgaa ttagctgcaa aaaatttaga
    19141 tttgtacgat attaaattag aatttggtcg tgatattcaa ggtaatgtaa tgctgattga
    19201 tgagatttca ggcgggaata tgcgtgttta tcaaaatggg aaatatttag aacccttgca
    19261 attagaacaa gtatttttag cttaaaaatt atcttgataa taacttaaat aatcctttgc
    19321 ctccaagatt aaataaagca tcgatgatac ttaaattagg ttgaaactca aatctatctg
    19381 agaaaacttg cagataagta atcggctcgt aagttttttc tacttgaatt ttacttactt
    19441 cgttattata gacaattgct ttttcaattt gtaatttctt taaaatccat tggtgtaatt
    19501 caatattaaa agtacttaag ctgtcaaatt gattgtcata gaagtatttt aattcttctt
    19561 cgtagtattc aaaatatggc gaacgacgat aggctgaaac tatagaattc caatggtcct
    19621 tttgccattt ttcagcataa gaaattttta catcagcata ttttcttctt tcattttgtt
    19681 gatgctgtaa tggaatactt aatcgtaaaa cgccatttgc acctaaaata taagttctgt
    19741 tttttagcgt tttttttgtg tatattgaat tcgttgtaag tgtaatattt tgatgtataa
    19801 tattttgata aaaggaaatt ggtgctaggt ataaaggctc cagagttagt atatcattca
    19861 ttatgcaaag atattctttt ataaatagca attttagtat tttttttcat tgcaagaaat
    19921 ttatatttct tatctttttg tttttcttcc gctagtattt tgagcagatt ttgtgttcga
    19981 tgattttggc tttgctgttc gagaaatagt gtttgttttt gaagaattac gagaatttac
    20041 agacttttca ttttttgaag ctaatttttt atgtgtccct ttttgagatt tggtcgagtt
    20101 gcccgttttt attgtatttt tattgcgttc tgttttgtta gttgtattat ttgaagaagc
    20161 tgattttgca ggaataacag cttttttttc ttccgaaatt gaactttcag tcaattgata
    20221 aattttattc atttcatctt cttcaagtat tcgccattct ccttcaggca aacccttcaa
    20281 agtgatattc ataatacgaa cacgttctaa tttggtaact tcataattgt aaaattcaca
    20341 cattctacgg atttgtctat ttaaaccttg aactaaaaat atcctaaatg tctttgtact
    20401 ttctttaata attctacatt ttttagtagt tataccaagc attggaactc cttgtgccat
    20461 accattaatt gcagcatctg tgagtggttt atttacagta accacatatt ctttttcatg
    20521 attatttcct gctcgtaata ttctattgac catatcgcca tcattagtca agaatattag
    20581 tccttgagag tctttgtcta atctgccaat tggaaaaatg cgaacaccgt aatttacaaa
    20641 atcaacaata ttattcttaa cacccttttc agtagtcgat acaattccga caggcttatt
    20701 aagtgctata aaaattacat cttctttttc caaaggctct aattcttgac catttacttt
    20761 tactatatct ccagcaaaaa cttgatcccc tttttgagct ttctttttat taataaatac
    20821 aacaccacgc tcaattaatt tatcggcttc tcgacgagaa catagtccac tttctccaat
    20881 aaatttattg agccagacac cacttccttt ttgcattttt taccgaaaga taaaactttt
    20941 catttaaagt atttctttaa aaagcagatt gtcgaaaact gtttatttat tacatttcaa
    21001 tagttaatat cttatgcagt ttatctataa ataaattgct ttttaattgg gaagtagaac
    21061 tgattttggt aataattatt aatttttccc tttgacaata tgatatttgc aaactacatt
    21121 taactataca attattttga gttttgcata atgatttaat aatacatttt ttgaaattaa
    21181 aattggatag ttttatccgc ttttatatct ttaacatatg ttttcaaaag cttgtgaata
    21241 cggaattaga gcaacggttt atatagccga ccaatctaaa caaagcaata gagccatatt
    21301 aaaagatatt gctaaagaaa ttgattctcc agaagcattt actgcaaaaa ttcttcagca
    21361 gttggttaaa agtggaatcg tacattctgt aaaaggcatt aatggtggtt ttgaaatttc
    21421 taaagataaa attagtaact taaagcttgc agatgtcgta ttagcaatag atggtgaagg
    21481 tatttatacc aattgtgcat tcggtttaaa aaattgttct gaacaatttc cttgtccagt
    21541 acatcataaa tttaaaccta ttaaggcaaa tattcgcaaa atgttagaag gaacaagtat
    21601 caatgatatg tcagataaat taaaagatgg attggccttt ttaaaactag aaaattatta
    21661 attataaata tattattatg aatggtggac aaattatcgc aaaagtactg caaaatcatg
    21721 gggttaaata tctattcaca ttatgtggag gtcatatttc cccaattttt gtaagtgcag
    21781 aacagttagg tattcgtgta attgatacac gccatgaagc aaacgcagtt tttgcagccg
    21841 atgcggtatc tcgcttaacg ggaattccag gcgtagcgac tgttacagca ggaccaggtt
    21901 taacgaatac aattactgcg gttaaaaatg cttatttagc acaatcgcct ttggttttat
    21961 taggaggtgc agctgcaact attctaaaag gcagaggctc tttgcaggat attaatcaaa
    22021 tgagtattat gcgtccaaat gtaaaatggg ctgcaacaat ttctaaagta aaagatattg
    22081 ttcctacatt agaaaaagca tttgaagtgg cacaaagagg tgtacctgga cctgtttttg
    22141 tagaatgtcc aatagatatt ttatacggag aagaattagt gcgtgaatgg tatggtgcaa
    22201 agagcaaaga cgttccgcct aaaaacattc aagagcgtgc cattcaatgg tatatcaatt
    22261 ggcatgcaca aagaattttt gaagataaag ataaagttac ctttggacaa cctcgtccag
    22321 tttattttcc aaatcacaac agctctgata ttaatagtgc ggtagattat ttgaaaaaag
    22381 ctaaaaatcc acttttggtt ttaggaagtg gagcgatgat gcaacctcaa aaagcacaac
    22441 aattagctga tgcggtaaat aaattaggta ttccaactta tttgagtgga atgggcagag
    22501 gtttattagg caaagatggc gtgttgcaaa tgcgacataa acgtaaagaa gccattaaag
    22561 atgctgattt aattatattg gcaggagtgc ctaatgattt tagattagac tatggtaatc
    22621 atattggaca tagaccattt atctctatta atagaagtac tgaagatttg aataaaaata
    22681 aaaagccaaa tcttggtatt aatgccgacc cacaagattt tttaattgaa ttagccaata
    22741 aatatcagct taaaaataat agtgcttggt tatcaaaatt gaaagataga gataatgctc
    22801 gtgagaaaaa tatcgatgag caagcagctg gtaaagttaa tggcattaat ccagttcagt
    22861 tatttagaga aatggatcca atgttgagtg ataatactat tttaattgct gatggtggtg
    22921 attttgttgg aacagcagca tatactttaa aagcacgctc tccactttct tggttagacc
    22981 caggtgtttt cgggacatta ggtgttggtg caggttttgc attgggtgcg aaattagttt
    23041 atcctgaaaa agaagttatc gtagaagatc acatttacag attg
//
LOCUS       DAOQCW010000027        22561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_834_length_22561_cov_5.427175, whole genome shotgun sequence.
ACCESSION   DAOQCW010000027 DAOQCW010000000
VERSION     DAOQCW010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 22561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22561
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_834_length_22561_cov_5.427175"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1335
                     /locus_tag="PLS16_03915"
     CDS             <1..1335
                     /locus_tag="PLS16_03915"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNJ60260.1"
                     /translation="KKKICYAPFNNLHFQINGNVSACSFNYDFIIGNVNENTIKEIWT
                     GEKAQVFRDKLGNYNFELCKSCEHVLTAKNFNSFPPLKYDMYADDDINYPNQMSFEMS
                     DLCNFECVMCSENFSSLIRKRKGFAPQKFSYPERFFTELQEFLPHLKMATFIGGEPLL
                     IKAYFRIWEDILKVNNKCTIHIQTNASYLPPRFLEMLESGQFDVGISLDATEKETFEK
                     IRINSNFKDIIENIIILKSYMDKNKVNLNLNFCPLTQNWQDIPKIVQYANNLNIPLKI
                     VNVENPRNLSLQHRNPSFLAQTISYLEINTNFNPNGIIQEKNVQSYKQYIGQLKYLQK
                     SAIEREKYYSENYLEISDKFRELFKTSNLFNNFNIEQRNELQNNIISYIDELITENEH
                     LKIRTIFRVYYALFRFKHTNNGDASTNFEYGQNILKEVVKEFYLLEKDELND"
     gene            1418..2386
                     /locus_tag="PLS16_03920"
     CDS             1418..2386
                     /locus_tag="PLS16_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60261.1"
                     /translation="MNKFSLLLIFVFFFNAYIFAEEATEYQPTPIIFKHIDNDTLDNY
                     IQKKGKTAFLHQLDSIIGDLSKLIKFSTANIKDNTIAFELKAINQHFKLGMDSMLYFP
                     QEVQIDSGINRVYGIMMGNTKVGIQFIEAAKKENVDFNKVFQEEVYKPDVRAGLYIFC
                     NYLDNISEAALDTLYHAALVYPNNSSTRVDFYYALSEFDRNCGNPTNNSKYYKDKLYE
                     QIKDNYLLEKALSDTNKLNSFNYSQYNLTQKYRFLAYSLYSKNFDIKNNQKEIVYLLS
                     SQNSDGGFKEYNIASSREPIRSKIEPTIYAFWALLEFREQINKIYE"
     gene            2584..3867
                     /gene="aceA"
                     /locus_tag="PLS16_03925"
     CDS             2584..3867
                     /gene="aceA"
                     /locus_tag="PLS16_03925"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF011645.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HNJ60262.1"
                     /translation="MATRQERIDALKKDWAENPRWKGIKRPYTAEEVVSISGSVQVEY
                     TLAKNGAQKLWDRLHSRHWVAGLGALTGNQAIQEVQAGMPAIYLSGWQVAADANLGMS
                     MYPDQSLYPADSVPAVVRRINNAFARRDEIQWLNQDGDVDWFAPIVADAEAGFGGNLN
                     AFELMKGMIQAGAAGVHFEDQLASAKKCGHLGGKVLVPTQEAINKLAAARLAADSMGV
                     PTLVIARTDAEAATLITSDIDPRDHKFILNKERTSEGFYNVKNGVEAGIDRAVSYAHY
                     ADLIWMETGNPDLGMAREFAAEVHKQHPGKMLAYNCSPSFNWAKFMDVKQMETFREDI
                     ADMGYKFQFITLAGFHALNSSMFDLSKNYMARGMAGFSEMQQREFAMEADGFKAIKHQ
                     SFVGTGYFDALQNIVQQGQSSTTALAGSTEAEQFH"
     gene            3994..4752
                     /locus_tag="PLS16_03930"
     CDS             3994..4752
                     /locus_tag="PLS16_03930"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ60263.1"
                     /translation="MDKQHTEKVKTYYDKMTRSYLQIYGEVIQAFRPSRTKNLLKYIA
                     ISSGLKKNMNILDAGCGVCGPAIYFAKNYKVNIDAITISPIQVEIAQQEINKNKLGKY
                     INVIEGDYHKLNQYYTVPNKYDLIFFLESLGHSTQPETIINLAFNLLKKGGKIYIKDF
                     YKKNSINPNTQQNIDQVIQSINKHYEYNTLELTNIINSIRSAGFEIDFIKKFDFKDDI
                     SIRFEFEQHHNIDIFGEIPEFYPAEWLEICCTKT"
     gene            4845..6539
                     /locus_tag="PLS16_03935"
     CDS             4845..6539
                     /locus_tag="PLS16_03935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNJ60264.1"
                     /translation="MKKILLIILSVCSFFMYAQNTCKPHKKQVKIRVFGDSWAHFPVI
                     YHAYDSALVQYGFPEYVSKGDFTVLISMNSEAFLNPFAKALWMPQIEQDGRDNIPLMI
                     ISLLGNDIAFKFKKNFPVSILDAPFAEAKTNMDTIFDFIHEKCPDMQILWMCYDYPNF
                     VDPIIDYPWNPYREIWDNKGQFTPAEINPFVKYMAQLQDSLLQVWNRPYIHFVNNVGL
                     MQYMYGQPTPLRVAPFGTYPPRSVPFPGGDENYPTPYAAMGLGGIDTYHLSPQGFTFL
                     ASYQMRKFFMEQLRKERDTTIQSMGGEFDGYVTQNGIIGNEHITVGKNAQDVQHKGIF
                     TFNTEVIPDNKKIIKASLFIRRQSGNYNSFRTNYYPTNFKLDIKSGNIGINPTIEAED
                     FNASTSLEDITCALGNTSRDNYSIRFDIKGAALDYINKNGFTQFRLSITDTASGESMI
                     NYFTGDTTEFEAPYLDLYYDPIEIISAIKEIKNEKVTIFPNPVKNTVNINDLNKTSLK
                     NKVAIRIFSTDGREIFHIEKNKNDLIQLNLQSLNSGIYQIRIDDGEQFITDKIIKI"
     gene            6822..9002
                     /locus_tag="PLS16_03940"
     CDS             6822..9002
                     /locus_tag="PLS16_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HNJ60265.1"
                     /translation="MSKTRFNSIAAFSSRQKVNVELPSEKISDFFASAVFSDKQMKLY
                     LSNDAYKKLQHCIRENIKVDRDLADQVASAMKAWALKLGATHYTHWFQPLSGTTAEKH
                     DSFFIPDGTEGIEEFSAGALIQQEPDASSFPNGGIRVTFEARGYTAWDLTSPAFIMTV
                     GNGKTLCIPTIFISYTGETLDYKAPLLKSIKSINQAATDVCQYFDKEVKKVNVTLGWE
                     QEYFLVDTAMYYARPDLLMTGRTLLGRRSPKGQQMEDHYFGAIPERVYAFIRDFEIEC
                     LKLGIPIRTRHNEVAPSQFECAPMFEEVNLAVDHNSLLMDVMDRVSSKHKLKALFHEK
                     PFAGVNGSGKHNNWSLATNTGVNLLSPGKTPRTNLQFLTFFINTIAAVNKHEDLLRAS
                     VASQSNDHRLGANEAPPAIISVFTGSFLKDVLDLIEERVDEKKFGEQDNVSLRLDIHN
                     SIPDILLDNTDRNRTSPFAFTGNKFEFRAVGSSANCALPMTVLNAIVAEQLIIFKKEV
                     DEYIKGGDYKDVAILKVLRKTIKDSKRILFEGDNYSDEWHKEAKKRGLSNNKTTPDAL
                     DAFVTKKAVKLFEDLQIYSHREIEARYEIALESYSKSVQIESRTLGDLIQTQIIPTCI
                     EYQNKLATNVKHLKDLGISSNPQLATLKKLAESIEQLDKLTNEMTELRIKGNALTGKK
                     QANLYCDKVRPASEEIRNIADELESIIENELWPLPKYSEMLFFK"
     gene            9264..13796
                     /gene="gltB"
                     /locus_tag="PLS16_03945"
     CDS             9264..13796
                     /gene="gltB"
                     /locus_tag="PLS16_03945"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HNJ60266.1"
                     /translation="MNLTTEKTGLYVPEMEHDACGIGFVAHLKGEKSHRIIKDALTML
                     ENMEHRGACGCEENTGDGAGILMQIPHNFYKNEVAKIGITLPEEGKYGTGFIYFPQDD
                     TKRNECKTFIQQTAEKLNFTVLGYRKIKTDNSPIGPTAKSTEPVIEQLFIQPNFNFNE
                     AIELERKLYILRAHTVREINYILGGKDHFYYTDLSCRTVIYKGQLTTFQVRQYYPELN
                     DESVESALALVHSRFSTNTFPKWRLAQPFRYIAHNGEINTLQGNLNWMRTREHLLECD
                     VYSKDEIDMIMPICYRDQSDSASLDNVVELLYLTGRSLPHAIMMLIPEAWQDNDQMED
                     WKKAFYEYHDCLMEPWDGPASICFTDGKMVGATLDRNGLRPSRFVVYNDGRVVMSSEA
                     GALPTEEEKVIQKGRLQPGKMFVINMEEGRIISDDELKKDICTQYPYREWLNKNKITF
                     SDLPNSPIKEENLSADELIQQQLVFGYTSEDVRVIITPMAETAYEPIGSMGTDTPLAV
                     LSDQYQNLSNYFKQLFAQVTNPPIDPIRERMVMSTFTSLGGASSILTTNEDHCKQIAI
                     QQPILTDDDIAKLRYIKHPDFKVITIQALFTASGKRGALERGIDRICKEAEEAILNGN
                     NILIISDKGANEDLAPIPSLLAVGAIHHHLIRTKIRINCSLVVESGDIREVHHMATHI
                     GYGATAINPYLAYQTIYDLNKDILKHLGKPDEKLVANFIKASSNGLLKVFSKMGISTH
                     QSYHGSQIFEIVGLSKKVVDKCFSGSISRLDGIGFDEIAIEVLGRHALAFPEAEHAKQ
                     RLEVGGNYQWKRRGEAHLFNPETIHLLQHSTRKNDYNVYKKYAEKINDQVEKAITLRS
                     LFDFDFSKTTPIPIEEVEPAENILKRFATGAMSFGSISYEAHSTLAIAMNRIGGKSNS
                     GEGGEDESRYEVKANGDSERSAIKQVASGRFGVTSYYLSNADELQIKLAQGAKPGEGG
                     QLPGHKVDEWIGRVRHSTPGVGLISPPPHHDIYSIEDLAQLIFDLKNANPKARINTKL
                     VAEAGVGTVAAGVAKAKSDVILISGYDGGTGASPISSIKHAGLPWELGLSEAHQTLVR
                     NNLRSRVVLQTDGQLRTGKDIAYATLLGAEEWGIATAALIVEGCIMMRKCHLNTCPVG
                     VATQDPVLRARFTGDPQHVINFFTFIVNDLREWMAKLGFRTINEMVGQTQLLRLRDNI
                     DHWKYKHLDLSPILFKEQANNEVGIYKQMRQTHPIDDALLDKKMIEAANKSLEDQTPT
                     FAEFNIINTDRTVGALLSYNISSKYKGIGLPEDTINFKFIGSAGQSFGGFAAPGIKFE
                     LEGEANDYFGKGLSGGKLIAYPSTKATFKASENMIIGNVAFYGATNGEAYINGLAGER
                     FCVRNSGVKAVVEGVGDHGCEYMTGGLAIILGKTGRNFAAGMSGGVAYVYDIENTFPK
                     NVNKEMVELEALDTEDLALLQHHIERHLEYTKSEIAKNILDNWDVTSKHFIKVMPTDY
                     KKVLDAKKASAKELSI"
     gene            13820..15310
                     /locus_tag="PLS16_03950"
     CDS             13820..15310
                     /locus_tag="PLS16_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HNJ60267.1"
                     /translation="MGKITGFLEFNRELPKHKDPKERIHDYKEFYLPYPEEKTRNQAA
                     RCMDCGVPFCHNGCPLGNIIPEFNDAVYKGDWELAAETLLSTNNFPEFTGRICPAPCE
                     TACVLGINQPPVAIEYIEKSIIEKAFELGYVQPNIPTERTNKNIAIVGSGPAGLAAAT
                     QLNKAGHNVVVFERADRVGGLLRYGIPDFKLQKDVVERRIDLMEKEGVIFKTNTNIGV
                     DITLNELQEKFDAVVLSGGSTVARDLPISGRELKGIYPAMEFLSQQNKRVAGIPTEID
                     HRGMPYLNGDLLATGKNVLVIGGGDTGSDCVGTSNRHGALSVTQIELLSKPPIERAES
                     TPWPEWPMMLRTSSSHEEGCDRQWAILTKEFIGNENGELTGLKIVEIEWKEIEGKQQM
                     SEIEGTERILPCELVLLAMGFVHPQHEGMLEQMPLEKDGRGNLKSKRYKTNIDKVFTA
                     GDMRRGQSLVVWAISEGRECAREVDIFLMGESKLEAKDRGIFQVNK"
     gene            15435..16019
                     /gene="scpB"
                     /locus_tag="PLS16_03955"
     CDS             15435..16019
                     /gene="scpB"
                     /locus_tag="PLS16_03955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNJ60268.1"
                     /translation="MEDIQRNIEAILFAAENPVAIDDLLKHLNKENPEPYTTEYISEI
                     IGLIQEKYQNELFPFQIGQVGGGYQFLTKPAFHEVVSAYLNRNTVKRLTQAALEVLAI
                     IAYKQPVTKNLIESIRGVNADYAVQKLMEKELIEIAGRSEEVGKPLLYKTTQQFLDYF
                     GINSIDEMPKLKEFEELQNEIGEKENIENISQQN"
     gene            15979..16866
                     /locus_tag="PLS16_03960"
     CDS             15979..16866
                     /locus_tag="PLS16_03960"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNJ60269.1"
                     /translation="MKKKISRTSPNKISRSKTIDRKDNKIDLFEKRIDDAVKNFKTPK
                     NDNRSSSTEGGLRLNKFISNAGICARRKADEFIGQGFVTVNGNVVLEMGYKVQEKDIV
                     KFKGKKVTPIKFSYILLNKPKSVITTTDDELNRKTVLDIVQGAAKERIYPIGRLDRNT
                     TGVLLLTNDGELAQKLSHPSSNVTKVYQVELNKPLAEKDRLAIKEGLELEDGIAQIDE
                     IEYTLPRGDDKHVGIELHIGKNRIVRRIFEHLGYEVEKLDRVLYAGLTKKNVPRGKWR
                     YLSEKEIIFLKHFAGHEKK"
     gene            17109..17927
                     /gene="pyrF"
                     /locus_tag="PLS16_03965"
     CDS             17109..17927
                     /gene="pyrF"
                     /locus_tag="PLS16_03965"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNJ60270.1"
                     /translation="MTRKQLIQQIKKKKSYLCVGLDTDLDKIPKHLLKYKQPIVEFNK
                     QIIEATQDLCVAYKPNIAFYEQFGAKGFEFLEKTLSYIPENIFTIADAKRGDIGNTSK
                     MYAKAFFETLNFDSITVAPYMGSDSIKPFLDYKNKWTIILGLTSNTGSQDFQFLKSNK
                     KPLYQQVIETAMQYGNEGNTMFVIGATQEKSIKKIRTFCPEHFFLVPGVGAQGGSLEK
                     ISEFGLNQDIGLLVNSARNIIYASNGKDFAEKARDEAQKIQQEMQAILKRKQLI"
     gene            17940..18914
                     /gene="galT"
                     /locus_tag="PLS16_03970"
     CDS             17940..18914
                     /gene="galT"
                     /locus_tag="PLS16_03970"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HNJ60271.1"
                     /translation="MSELRYNPLLNTYTIVAANRQNRPNFPKNWCPFCPGEGKKVPNN
                     YEVFVYPNDFPALDYQNKVDNETTNEIYTKEAAKGACEVILYSPNHTANLYDLSDAHV
                     LKLIKTWIERFNFHKNFDDVKYIFPFENRGKEVGVTMPHPHGQLYAYPFIPQKIETEL
                     INAKNYFSKHQINLFDAMLKEEIEFQQRVIFETDTFLVYLPFFTDYPYGIFIVSKKER
                     LYISDFNEEEQLELSIVLKKVVRMFDNLFSKTFPFMMCIHQGVVNSTEYSEKTQRQFY
                     RFHIEFYPPLRDENAIKYYASSEMGAWAAANTKLVEETAKELRAALPQ"
     gene            complement(18966..20234)
                     /gene="glyA"
                     /locus_tag="PLS16_03975"
     CDS             complement(18966..20234)
                     /gene="glyA"
                     /locus_tag="PLS16_03975"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNJ60272.1"
                     /translation="MERDKKIFSLINDELERQTTGLELIASENFTSKQVMEAMGSCLT
                     NKYAEGYPGKRYYGGCEVVDQIEQLAIDRLCELFGAEYANVQPHSGAQANASVFLACL
                     KPFDTILGFNLSHGGHLSHGSPVNFSGKHYNPVFYGVEQETGLIDMDKVEALAKEHQP
                     KLIVCGASAYARDWDYARFRAIADSVGALLMADIAHPAGLIAKKKLNDPMPHCHIVTS
                     TTHKTLRGPRGGIIMMGKDFENPFGEKTPKGELKMMSQILNTGVFPGMQGGPLEHVIA
                     GKAVAFGEALTDEYNTYTEQIIKNAQAMANAFIDRGYKIISGGTSNHLMLIDLRNKNI
                     SGKDAERALGYADITVNKNMVPFDDKSPFVTSGIRIGTAAITTRHLMEQDCVQVVDWI
                     DTVLMNQADTDLILDIKKKVNKFMEDFPLY"
     gene            complement(20335..20697)
                     /locus_tag="PLS16_03980"
     CDS             complement(20335..20697)
                     /locus_tag="PLS16_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60273.1"
                     /translation="MEILGENNPISDFEDIDKGKTAAVVAYLTIIGLIIAFVININKK
                     STFASYHIKQAIGLTLTGFTLSIIERIVHNGFILGVLSLGLLVLWIMGIVNAITEKQK
                     PVPLLGEKYEEFFKNLNV"
     gene            complement(20876..21157)
                     /locus_tag="PLS16_03985"
                     /pseudo
     CDS             complement(20876..21157)
                     /locus_tag="PLS16_03985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
     gene            complement(21275..21505)
                     /locus_tag="PLS16_03990"
     CDS             complement(21275..21505)
                     /locus_tag="PLS16_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60274.1"
                     /translation="MKTALQIDITFDQILSMVKQLPRQQKIKLSKELEKEAIDSKLTR
                     LLKSFRTKELDLKTINSEVETVRQEIYAKQKH"
     repeat_region   21614..22530
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=21614..21649
                     /rpt_unit_seq="atctacaatagtagaaattatcggccattttcagac"
BASE COUNT         8232 a         3452 c         3657 g         7220 t
ORIGIN      
        1 aaaaagaaaa tatgttatgc tcctttcaat aatttacatt ttcaaatcaa tggcaatgtt
       61 tcagcttgta gttttaatta tgactttata attggcaatg taaatgaaaa tacaataaaa
      121 gaaatttgga caggtgaaaa agctcaagta tttagagata aattaggtaa ctataatttt
      181 gaattgtgta aatcttgcga acatgttctg actgcaaaaa atttcaattc atttcctcca
      241 ttaaaatacg atatgtatgc tgatgatgac ataaattatc ctaatcaaat gtcatttgaa
      301 atgagtgatt tatgcaattt tgagtgtgtt atgtgtagtg aaaatttctc ctctttaatt
      361 agaaaacgaa aaggatttgc tcctcaaaaa ttcagttatc ctgaacgctt tttcacagaa
      421 ttacaagaat tcctcccaca tcttaaaatg gcaacattta ttggtggaga accattattg
      481 ataaaagcct atttcagaat ttgggaagat attttaaaag taaataacaa atgtactata
      541 catatacaaa ccaatgcaag ctatctccct cctaggtttc tagaaatgtt agaatctgga
      601 caatttgatg ttgggatttc attagatgca accgaaaaag aaacatttga aaagattaga
      661 atcaattcta attttaaaga tataattgag aatattatta tcctaaaaag ttatatggac
      721 aaaaataagg ttaatttaaa cctaaacttt tgtccgctta ctcaaaactg gcaagatatc
      781 cctaaaattg ttcaatatgc taacaactta aacattccat taaaaatagt aaatgtagaa
      841 aaccctagaa acctatccct acaacataga aatcctagtt tcctggctca aacaatatcc
      901 tacttagaaa taaatactaa ttttaatcca aatggtataa ttcaagaaaa aaatgtgcaa
      961 tcgtacaaac aatatattgg acaattaaaa tatttacaaa aatctgcaat tgaaagagaa
     1021 aaatattact cagaaaatta cttggaaatt tcagataaat ttagagaatt atttaaaact
     1081 agtaatttat ttaataattt taatattgag caaagaaatg aattacaaaa caatatcata
     1141 tcttatattg atgaactaat cactgaaaat gagcacttaa aaattagaac aattttcaga
     1201 gtctattatg cattatttag atttaaacac accaataatg gcgatgcttc tacaaatttt
     1261 gaatatggac aaaatatttt aaaagaagtt gtaaaagaat tttatttatt agagaaagat
     1321 gaacttaatg attgatgcct tcacaaaaca atggcattaa ttttgagtat tatatttgac
     1381 tattaattat tttttctatt ttagtaaaaa aaacaatatg aataaattta gcctactctt
     1441 aatatttgta ttctttttca atgcttatat ttttgctgaa gaagcaactg aatatcaacc
     1501 aacacctatt atatttaaac atatagacaa cgatacatta gataattaca ttcaaaaaaa
     1561 aggcaaaaca gcatttttac atcaattaga ttcaataatt ggcgacttat ctaaattgat
     1621 aaaattttct actgctaata ttaaagataa tacaatcgct tttgaactta aagctatcaa
     1681 tcaacatttt aaattaggaa tggactctat gctgtatttt ccacaagaag tacaaataga
     1741 tagcggcatc aatagagtct atggaattat gatgggaaat actaaagttg gaattcaatt
     1801 tatagaagca gctaaaaagg aaaatgtaga tttcaacaaa gtatttcaag aagaagttta
     1861 taaaccagat gtaagagctg ggctttatat tttttgtaat tatttagata atatttcaga
     1921 agcagcacta gacacgctct atcatgctgc cttagtttat cctaataatt cttcaactag
     1981 agttgatttt tactatgcat tatctgaatt cgacagaaac tgtggtaatc caacaaacaa
     2041 ttctaaatat tataaagata aattatatga acaaataaaa gataattact tattagaaaa
     2101 agcactctcg gatactaaca aattaaattc atttaattat tctcaataca acttaactca
     2161 aaaataccgt ttcttggctt acagccttta cagtaaaaat tttgatatta aaaataacca
     2221 aaaagaaatt gtttatttat taagtagtca aaattctgat ggtgggttta aagaatataa
     2281 tatagcttct tctcgagaac ctattagaag taaaattgaa ccaactatct atgcattctg
     2341 ggcattatta gaatttagag agcaaataaa taaaatatat gaatagttta taaattattt
     2401 tttattttat ttaatatata taagtagcaa tatcaaagtt ttattttcat ctaataaact
     2461 taacttctta ctactacttt cttcaaaatc tataaactaa tattaacata aacttttaga
     2521 atgattgctt atttttgtag cattacttga tattatcatt ttaaccaaaa aaattaaata
     2581 gaaatggcta caagacaaga gagaattgac gctctaaaaa aagattgggc tgaaaaccca
     2641 agatggaaag gtattaaaag accgtacact gctgaagaag tggtatcaat tagtggttct
     2701 gtacaagtag aatacacttt agctaaaaat ggtgctcaaa aattatggga cagattacac
     2761 agccgtcatt gggtagctgg tttaggtgca ttaacaggta accaagcaat ccaagaagtt
     2821 caagctggta tgcctgctat ctatttatca ggatggcaag tagctgctga tgctaactta
     2881 ggtatgtcta tgtacccaga tcaatcatta tatcctgctg actcagttcc tgctgtagtt
     2941 agaagaatta acaatgcatt tgcaagaaga gatgaaatcc aatggttaaa ccaagatggt
     3001 gatgttgatt ggttcgctcc tatcgttgct gatgctgaag ctggtttcgg tggaaactta
     3061 aatgcttttg aattaatgaa aggtatgatt caagctggtg ctgctggtgt tcactttgaa
     3121 gaccaattag cttctgctaa aaaatgtggt cacttaggtg gaaaagtatt agttccaact
     3181 caagaggcaa tcaataaatt agctgctgct cgtttagctg ctgactctat gggggttcca
     3241 acattagtga ttgcaagaac tgatgctgaa gcagctacat taatcacttc tgatattgac
     3301 ccaagagacc acaaatttat cttgaataaa gaaagaactt ctgaaggttt ttataatgta
     3361 aaaaatggcg ttgaagcagg tatcgacaga gctgtttctt atgctcatta tgctgactta
     3421 atttggatgg aaacaggaaa tccagattta ggaatggctc gtgagtttgc tgctgaagtt
     3481 cacaaacaac atccaggtaa aatgttagct tacaactgtt caccttcatt caattgggct
     3541 aaatttatgg atgttaaaca aatggaaact ttccgtgagg atatcgctga tatgggttat
     3601 aaattccaat tcattacatt agctggtttc cacgcattaa actcttcaat gtttgattta
     3661 tctaaaaatt atatggcaag aggtatggct ggtttctctg aaatgcaaca aagagaattt
     3721 gctatggaag ctgatggttt caaagctatc aaacaccaat ctttcgttgg aactggatat
     3781 tttgatgcat tacaaaacat cgttcaacaa ggacaatctt caactactgc attagcagga
     3841 agtactgaag ctgagcaatt ccattgataa tatattttca aatagattca aagcccgact
     3901 ttagtcgggc ttttctattt tttaatcaaa aataagatac aaatatccta tttaataaaa
     3961 tatacttata ttgtactaaa ggaaaagtat tttatggaca agcagcatac agaaaaagta
     4021 aaaacttact acgataaaat gactcgctcc tatctacaaa tatatggcga agtaatacaa
     4081 gcattccgac caagtcgaac aaaaaatcta ttaaaataca ttgcaattag cagtggatta
     4141 aagaaaaaca tgaatattct agatgctgga tgtggggtgt gtggtcctgc aatatatttt
     4201 gcaaaaaatt acaaagtaaa tattgatgct attacgattt ctcctataca agttgaaatt
     4261 gcacaacaag aaatcaataa aaataaacta ggaaaataca ttaatgttat cgagggcgat
     4321 tatcataaac ttaatcaata ttatacagta ccaaataagt atgatttaat cttttttcta
     4381 gagagtctgg ggcattctac acaacctgaa acaattatca atctagcttt taatttgcta
     4441 aaaaaaggag gaaaaattta catcaaagat ttctacaaaa aaaatagcat caacccgaat
     4501 actcaacaaa atattgacca agtcatacaa tctataaata aacactacga gtacaatact
     4561 ttagaattga ccaatattat aaattcaata aggagtgcag gatttgaaat tgattttatt
     4621 aaaaaatttg actttaaaga tgacataagt attcgattcg aatttgagca acatcataat
     4681 attgatatat ttggagaaat tccagaattc tatcctgctg aatggttaga aatttgctgt
     4741 acaaaaacat aatcatttaa tttttaacaa aattttaaaa tttaacatgc aatgttttaa
     4801 attttagtta aatctgtcta tttttagata gtaaaaattc taacatgaaa aaaatattac
     4861 tgattattct atctgtttgc tctttcttta tgtatgctca aaatacttgc aaacctcata
     4921 aaaaacaagt taaaattaga gtatttggtg acagttgggc tcattttcca gtaatatatc
     4981 acgcatacga ttctgcactt gtacaatatg gtttcccaga atatgtatca aaaggagatt
     5041 ttacggtatt aattagtatg aattctgaag cattcctgaa tccttttgca aaagcactat
     5101 ggatgcctca aatagaacaa gatggacgag ataatatccc attgatgatt atctcattat
     5161 taggaaatga tattgctttt aaatttaaaa agaattttcc agtctcaatt ttagatgctc
     5221 catttgcaga agccaaaaca aatatggata ccatttttga ttttattcat gaaaaatgtc
     5281 ctgatatgca aattctttgg atgtgttatg attatccaaa ctttgttgac ccaataattg
     5341 attatccttg gaatccttat cgagaaatct gggataacaa aggacaattt accccagcag
     5401 aaataaaccc ttttgtcaag tatatggctc agcttcaaga cagtttattg caagtttgga
     5461 atcgacctta catacacttt gtaaataatg ttggcttaat gcaatatatg tatggacaac
     5521 ctacgcctct tcgagttgct ccttttggta cttatcctcc tcgttcagtt ccttttccag
     5581 gaggagatga aaattatccg acaccttatg ctgcaatggg tttaggtggt atagacactt
     5641 accatttaag tcctcaagga tttactttct tagctagtta tcaaatgcgt aaatttttta
     5701 tggaacaatt aagaaaagaa agagatacta caattcaatc catgggtgga gaatttgatg
     5761 ggtatgttac acaaaatggt attattggta atgagcatat tactgttggt aaaaatgcac
     5821 aagatgtaca acataaaggt atttttacct ttaacacaga agttatccca gacaataaaa
     5881 aaattataaa agcaagttta tttataagaa gacaatctgg aaattataat tcatttagaa
     5941 caaattacta cccaactaat tttaaattag atatcaagag tggtaatatt ggtattaacc
     6001 caacaattga agccgaagat tttaatgcat caacaagttt ggaagatata acttgtgctt
     6061 tgggtaatac ttctagagat aattattcta ttcgttttga tattaaaggt gctgccttag
     6121 actatatcaa caaaaatggt tttactcaat ttcgactatc tataactgat acagcaagtg
     6181 gagaaagtat gataaattat ttcacaggcg acactaccga attcgaagca ccttacttag
     6241 atttatatta cgatccaatt gaaattatat cagccataaa ggaaattaaa aatgagaaag
     6301 ttactatttt tcctaatcct gtaaaaaata ctgttaatat taatgactta aataaaactt
     6361 cgctaaaaaa taaggtggca attcgtatat tttcaactga tggtagagaa atttttcaca
     6421 ttgaaaaaaa caaaaatgat ttaattcaat tgaatcttca atccttaaat agtggtatct
     6481 atcaaattag aattgacgat ggagaacaat ttatcactga taaaattatt aagatataag
     6541 taatttccta taaaaaacaa tctattttca ttacgaataa aagttatact gcaataatta
     6601 tcattctaat ctaaagtaat agcaataata aattaggaat tataaacttc taaattttca
     6661 ataaatttca taatatccat aaggaataat ttaaaaatca acaacattta ggtaaaattt
     6721 aggctacttt atttaaaata acaattaaat tacataaaat acttaaaaaa tattttgaac
     6781 tttgacataa aaaatagata tttacttaaa attattcaaa aatgtcaaaa acaagattca
     6841 attccatcgc tgcattttct tctagacaaa aagtaaatgt agaactacca agtgagaaaa
     6901 tttctgattt ttttgcaagt gctgtatttt cagacaaaca aatgaaactt tacctttcca
     6961 acgatgctta taagaaatta cagcattgta ttagagaaaa cattaaagtt gatcgagacc
     7021 ttgccgacca agttgcaagt gctatgaagg cttgggcttt aaaactaggt gcgacacact
     7081 atactcactg gtttcaacca ttatctggaa caactgctga aaaacacgat tcttttttca
     7141 ttcctgatgg aacagaaggc attgaagaat tttctgctgg tgcattaatt caacaagaac
     7201 ctgatgcatc ttcatttcct aacggtggca ttagagttac atttgaagca agaggttata
     7261 ccgcttggga tttaacatct ccagcattta ttatgacagt tggaaacgga aaaactttat
     7321 gcattccaac tattttcatt tcttatactg gagaaacttt agactataaa gctccattat
     7381 taaaatctat aaaatctata aatcaagctg caactgacgt atgccaatat tttgacaaag
     7441 aagttaaaaa agtaaatgtt acattaggct gggaacaaga atatttctta gtggataccg
     7501 caatgtatta cgctcgtcct gatttattaa tgactggaag aacattatta ggcagacgtt
     7561 ctccaaaagg acaacaaatg gaagaccatt actttggtgc tattccagaa agagtatatg
     7621 catttattag agattttgaa atagaatgtt taaaattagg cattccaatt agaactagac
     7681 ataatgaagt tgctccatca caatttgaat gtgcacctat gtttgaagaa gtaaatcttg
     7741 cagtagatca caactcatta ttaatggatg taatggacag agtttcttct aaacacaaat
     7801 taaaagcgtt atttcatgaa aaaccatttg ctggagttaa tggaagtggg aaacataata
     7861 actggagttt agcaactaat actggtgtaa atctattatc ccctggaaaa actccaagaa
     7921 caaacttgca gttcttaact tttttcatca atacaattgc tgctgttaat aagcatgaag
     7981 atttattacg agcatctgtt gcatctcaat ctaacgacca tcgtttaggt gctaacgaag
     8041 ctcctccagc aatcatttct gtatttactg gttcattcct taaagatgta ttagatttaa
     8101 ttgaagagag agttgatgaa aagaaatttg gcgagcaaga taatgtatct ttacgattag
     8161 atattcacaa tagtattcca gatattttgc tggacaatac agatagaaac agaacttcgc
     8221 catttgcatt tacaggtaac aaatttgaat ttagagcagt tggctcttct gcaaattgtg
     8281 ccttacctat gactgtgtta aacgcaatag tagccgaaca attaattatt ttcaaaaaag
     8341 aagttgatga atacattaaa ggtggcgatt ataaagatgt tgcaatctta aaagtactta
     8401 gaaaaacaat taaagattca aaacgtattt tatttgaagg cgataactat tctgatgaat
     8461 ggcataaaga agctaaaaaa cgtggattat caaataataa aacaacacct gatgcattag
     8521 atgcatttgt tactaaaaaa gctgtaaaat tatttgaaga tttacaaatt tattctcaca
     8581 gagaaattga agctagatat gaaatcgctt tagaaagcta ttcaaaatct gttcaaattg
     8641 aatctagaac tttaggtgat ttaattcaaa ctcaaataat tcctacttgt atagaatatc
     8701 aaaacaaatt agcaacaaat gtaaaacacc ttaaagattt aggcatttct tctaatcctc
     8761 aacttgctac gttaaaaaaa ctagcagaaa gtattgagca attagataaa ttgactaacg
     8821 aaatgactga gttacgcata aaaggaaatg cgttaactgg taaaaaacaa gctaatttgt
     8881 attgtgataa agttcgacca gcatctgaag aaattagaaa tattgcagat gaattagaat
     8941 ctattataga aaatgaattg tggccgttac caaaatatag cgaaatgctt ttctttaaat
     9001 aatattaata tctaagtcca ataattacaa aatctctgtt gcttactaat ctaaacaaca
     9061 gagattttta gtttaaacta aattcgtatt taaaaatttc aatcccttaa aaatattttt
     9121 ttttacatta aaataatttt tttttaaaaa aacaaggata aataaaaaac agaaactatg
     9181 taactttatt aaactttctg tttgtttttt tattgaacaa aggtaagtac ctaaatttat
     9241 atttttaata tttataaaca tatatgaact taaccactga aaaaaccgga ttatatgtac
     9301 cggaaatgga acatgatgct tgtgggattg gatttgtcgc acatttaaaa ggagaaaagt
     9361 cccatcgcat tataaaagat gcccttacta tgttagaaaa catggaacat agaggagctt
     9421 gtggttgtga agaaaataca ggtgatggtg ctgggatatt gatgcaaatt ccacataatt
     9481 tttacaaaaa tgaagtggca aaaatcggca ttactttacc tgaagaggga aaatacggaa
     9541 caggttttat ctattttcct caagatgaca cgaaaagaaa tgagtgcaaa actttcatcc
     9601 aacaaactgc tgagaaatta aactttactg ttcttggata tagaaaaata aaaacagaca
     9661 actctcctat tggaccaact gcaaaatcaa cggaaccagt tatagaacaa ttgtttatcc
     9721 aaccgaattt caactttaat gaagccattg aattggagcg taaattatac attttgcgtg
     9781 cgcacactgt tcgagaaata aactatattt taggtggaaa agaccatttt tattataccg
     9841 atttatcttg tcgcactgtt atttacaaag gacaattaac tacttttcaa gtacgccaat
     9901 attatccaga attaaatgat gaaagtgtag aatctgcttt agctttagta cactcacgat
     9961 tttctaccaa tacatttcca aagtggagat tagctcaacc tttccgatac attgcacaca
    10021 atggtgaaat caatacctta caaggtaatc tgaattggat gcgtacacgt gaacatttat
    10081 tagaatgtga tgtgtattct aaagatgaaa ttgatatgat aatgcctatc tgttatcggg
    10141 atcaatctga ctcagcttct ttagataatg tggtagaatt attatatcta actggacgtt
    10201 ctttgcctca tgctattatg atgttaattc ctgaagcttg gcaagacaac gaccaaatgg
    10261 aagattggaa aaaagcattc tatgaatacc acgattgctt aatggagcca tgggatggtc
    10321 cagcttctat atgttttaca gatgggaaaa tggttggtgc aacattagat agaaatggct
    10381 tacgtccatc aagatttgta gtgtataatg atggtcgagt tgtgatgtct tcagaagcag
    10441 gtgctttacc aacagaagaa gaaaaagtaa tacaaaaagg tcgccttcaa ccaggaaaaa
    10501 tgtttgtcat taatatggaa gaaggtcgaa ttatttcaga tgatgaactt aaaaaagata
    10561 tttgtacaca atatccatat cgtgagtggt taaataaaaa taaaataaca ttctctgatt
    10621 taccaaattc tccaataaaa gaagaaaatt tatcagcaga tgaattaatt caacaacaat
    10681 tagtttttgg atatacttcc gaagatgttc gtgtaatcat tacaccaatg gctgaaactg
    10741 cttacgaacc aattggttct atgggaacag atacgccact tgcagtactt tcagaccaat
    10801 accaaaattt atcaaattat tttaagcaac tgtttgctca agttaccaac ccaccaattg
    10861 acccaattcg tgaacgtatg gtaatgagta catttacatc tttaggtggt gcgagtagta
    10921 ttctaacaac aaatgaagac cattgcaaac aaattgcaat tcaacaacct attttgacag
    10981 atgatgatat tgcaaaactt cgttatatca aacatcctga ctttaaagtt atcactattc
    11041 aagctttatt tacagcaagt ggaaaacgtg gtgcattaga aagaggtata gatagaatat
    11101 gtaaggaagc tgaagaggca attttaaatg gtaataatat tctaattatt tcagacaaag
    11161 gtgcaaacga agacttagct ccaattcctt ctttattagc tgttggtgca attcaccatc
    11221 atttaatcag aacaaaaatt agaattaact gctctttagt tgttgaaagt ggcgatatac
    11281 gtgaagttca ccacatggct acgcatattg gatacggagc aactgctatt aatccatatt
    11341 tagcttatca aaccatctat gacttaaata aagatatact taaacattta ggcaaaccag
    11401 atgaaaaatt agtagcaaac tttataaaag catcaagcaa tggtttgtta aaagttttct
    11461 ctaaaatggg aatttctaca catcaatctt atcatggttc acaaatcttt gaaattgttg
    11521 gattaagcaa aaaagttgta gataaatgtt tctctggctc tatttctcgc ttagatggaa
    11581 ttggatttga tgaaattgca attgaagttt taggcagaca tgccctagca tttcctgaag
    11641 cagaacatgc aaagcaaaga ttagaagttg gtggaaatta tcaatggaaa cgtcgtggag
    11701 aagcacattt atttaatcct gaaacgattc atttattaca acattctacg agaaagaatg
    11761 attataatgt ttataaaaag tacgctgaaa aaataaatga ccaagtcgaa aaagcaatca
    11821 cacttcgtag tttatttgat tttgattttt caaaaacaac gcctattcct attgaagaag
    11881 ttgaaccagc cgaaaatatt ttaaaacgtt ttgccactgg tgcaatgtca tttggttcta
    11941 tttcttatga agcacattct accttggcaa ttgctatgaa tagaattggt ggaaaatcaa
    12001 attctggcga aggtggtgaa gatgaaagca gatatgaagt taaagcaaat ggcgactccg
    12061 aacgttcggc tattaaacaa gttgcttcag gaagatttgg agttacttct tattatttat
    12121 ccaacgctga tgaattacaa attaaattag cacaaggtgc aaaacctgga gaaggtggac
    12181 aattacctgg acataaagtt gatgaatgga ttggtagagt tagacactca actccgggag
    12241 taggtttaat ttctcctcct ccacaccacg atatttattc tattgaggat ttagcacagt
    12301 taatcttcga tttaaaaaat gccaatccaa aagctcgtat caatacaaaa ttagtagctg
    12361 aagctggtgt tggaacagtg gctgcaggag ttgcaaaagc aaaatcagat gtaattctaa
    12421 tctcgggtta tgatggcggt actggtgcct ctcctatttc ttctattaaa cacgctggtt
    12481 taccatggga attaggactt tctgaagctc atcaaacttt agtaagaaat aatttacgta
    12541 gtcgtgttgt attacaaact gatggtcaat tacgtactgg taaagatatt gcttatgcaa
    12601 cattattggg tgcagaagaa tggggcattg caaccgctgc tttaatagtt gaaggttgta
    12661 ttatgatgag aaaatgccat ctaaatacat gtcctgttgg tgttgcaaca caagaccccg
    12721 ttttgagagc aagattcaca ggcgaccctc aacatgtcat taatttcttt acatttattg
    12781 taaatgattt acgtgaatgg atggcgaaat taggctttag aactattaat gaaatggttg
    12841 ggcaaacgca acttttaaga ttaagagata atattgacca ttggaaatac aaacacttgg
    12901 atttatctcc aattttattc aaagagcagg caaataatga agtgggcatc tacaaacaaa
    12961 tgcgtcaaac acacccaatt gatgatgcat tattagataa aaaaatgatt gaggctgcta
    13021 ataaatcatt agaagaccaa accccaactt ttgctgaatt taatatcatt aatacagata
    13081 gaacagttgg agcattatta agttataata tttctagcaa atacaaaggt ataggcttac
    13141 ctgaggatac cattaatttt aaatttattg gaagtgctgg tcaaagtttt ggaggatttg
    13201 ctgcaccagg aatcaaattt gaattagaag gtgaagcaaa tgactatttt ggaaaaggct
    13261 tatcaggtgg aaaactaatt gcttatcctt ctacaaaagc tactttcaaa gcaagcgaaa
    13321 atatgatcat tgggaatgtt gcattttatg gtgccacaaa tggagaagct tatattaatg
    13381 gtctagctgg cgaacgtttt tgtgtaagaa attctggagt taaagctgtt gttgaaggcg
    13441 ttggagatca tggttgcgaa tatatgactg gtggtttagc cattatttta ggcaagactg
    13501 gtagaaattt tgctgctggt atgagtggtg gtgttgctta tgtttatgac atcgaaaata
    13561 ctttccctaa aaatgtaaac aaagaaatgg ttgaattaga agctttagat actgaagatt
    13621 tagcactttt acaacatcat attgaaaggc atttggaata tacaaaaagt gaaattgcca
    13681 aaaatatttt agacaattgg gatgttactt ctaaacattt tatcaaagta atgccaacag
    13741 attataagaa agtactagat gcaaagaaag cgagtgctaa agaattaagt atttaaaaag
    13801 agctataaag tataaattaa tgggaaaaat aacaggtttt ttagaattca atagagaatt
    13861 acctaaacat aaagatccaa aagagcgtat ccacgattac aaagagtttt atctgccata
    13921 tcctgaagaa aaaacacgta atcaagcagc aagatgtatg gattgtggtg ttccattttg
    13981 tcataatggt tgtccattag gcaatatcat tccagaattt aatgatgcag tttataaagg
    14041 cgattgggaa ttagctgctg aaacattatt atctacaaac aatttccctg aatttacagg
    14101 tagaatttgt cctgctcctt gtgaaactgc ttgtgtttta ggaatcaatc aaccgcctgt
    14161 ggctattgag tatattgaaa aatctatcat agaaaaagct tttgaattag gttatgttca
    14221 accaaatatt cctaccgaaa gaacaaataa aaatatagcc atcgttggtt ctggtccagc
    14281 aggtttggca gctgcaactc aattgaataa agctggtcat aatgttgttg tttttgaacg
    14341 tgctgacaga gttggtggtt tattaagata tggtattcct gattttaaat tacaaaaaga
    14401 tgttgtagaa cgtcgaattg atttaatgga aaaagaaggt gttattttca aaaccaatac
    14461 aaatatcgga gtagatatta cattgaatga attacaagaa aaatttgatg ccgttgtttt
    14521 atctggaggt tccactgttg caagagattt accgatttca ggcagagaat tgaagggcat
    14581 ctatcctgct atggaatttt tatctcaaca aaataaaaga gtagctggta ttccaactga
    14641 aattgaccat cgtggaatgc cttatcttaa tggtgattta ttagcaacag gaaagaatgt
    14701 tttagtaatt ggtggtggtg atactggctc agattgtgtt ggaacatcta accgtcatgg
    14761 tgcattgtcc gtaactcaaa tagaattatt atccaaaccg cctatcgaaa gagcagaaag
    14821 tacgccttgg ccagaatggc cgatgatgtt acgcacatca agttcgcacg aagaaggttg
    14881 cgaccgtcaa tgggcaattt taactaaaga atttattggt aatgaaaatg gtgaattaac
    14941 tggattaaaa attgttgaga ttgaatggaa agaaattgaa ggcaaacaac aaatgtctga
    15001 aattgaagga acagaaagaa ttttaccatg tgaattagtt ttattggcta tgggatttgt
    15061 acatcctcaa catgaaggaa tgttagagca aatgccttta gaaaaagatg gcagaggtaa
    15121 tttaaaatca aaaagataca aaaccaatat agacaaggtt tttactgcag gcgatatgcg
    15181 aagaggtcaa agtttagtag tttgggctat ctcagaagga agagaatgtg ctagagaagt
    15241 agatattttc ttaatgggcg aaagtaaatt agaagcaaaa gatagaggaa tttttcaggt
    15301 caataaataa ttgatagatt taaaccaaaa ttataaattc cattagatta aacatctagt
    15361 ggaattttta ctttatatta atacctaata tcatttcctt attattccat tttctttatt
    15421 aactttgcat acttatggaa gatattcaaa gaaatattga agcgatttta tttgcagcag
    15481 aaaatcctgt tgcaattgat gatttattaa aacatttaaa caaagaaaat ccagaaccat
    15541 atacgacaga atatatttcg gagataatag gtttaattca agaaaaatat caaaacgaac
    15601 tctttccatt ccaaattgga caagtgggtg gtggctatca atttcttaca aaaccagcat
    15661 ttcacgaagt tgtttctgct tatctaaata gaaatacggt aaaaagatta acccaagcag
    15721 ccttagaagt tttggctatt atcgcttaca aacaacctgt tacaaaaaat ttaattgaaa
    15781 gtatcagagg tgtaaatgct gattatgctg tacaaaaatt gatggaaaaa gaattaattg
    15841 aaatagcagg tagaagtgaa gaagtaggaa aaccgttatt atacaaaaca acacaacaat
    15901 ttttagatta ttttggcatc aactctattg atgaaatgcc gaagctaaaa gaatttgaag
    15961 aactccaaaa tgaaataggt gaaaaagaaa atatcgagaa catctcccaa caaaattagt
    16021 cgttcaaaaa ctattgatag aaaagacaac aaaatcgacc tttttgagaa aagaattgat
    16081 gatgctgtaa aaaacttcaa aacgcccaaa aacgataata gaagctcatc taccgaaggt
    16141 ggtttacgac taaataaatt tatatcaaat gctggtattt gtgccagacg taaagccgat
    16201 gagtttattg gtcaaggttt tgttactgta aatggaaatg ttgttttgga aatgggctat
    16261 aaagtacaag agaaagacat cgtaaaattt aaaggtaaaa aagttacacc aataaaattc
    16321 tcttacattt tactgaataa acctaaaagt gtaattacaa ctaccgatga tgaattaaat
    16381 agaaaaaccg ttttagatat tgttcaaggt gctgcaaaag aaagaattta tcctattgga
    16441 agattagata gaaatacaac tggcgtacta cttctaacaa atgatggtga attagcacaa
    16501 aaactttccc atccaagtag taatgtaaca aaagtttatc aagtagaatt aaacaagcca
    16561 ctcgcagaaa aagaccgcct tgccataaaa gaaggtttag aacttgaaga tggtatcgcc
    16621 caaattgatg aaatagaata tactttacct cgtggtgatg ataaacatgt tggcattgaa
    16681 ttacatattg gaaagaacag aattgtccgc agaatttttg aacatttagg ctatgaagtc
    16741 gaaaaactag atagagtttt gtatgctgga ttaaccaaaa aaaatgtacc tcgtgggaaa
    16801 tggcgttacc tttctgagaa agaaataatt ttcttgaaac attttgcagg acacgagaaa
    16861 aaatagtagt taggcattag tttttaggaa ttagaaatta gtttttaggc ataagtagta
    16921 agtttttttt aggcattagt ttttaggcat aagaagatac tgttaaattc taaaataaat
    16981 ttatccttcg aactatcaca aacaaagtaa gaacataata acctgaataa ttagcgtgta
    17041 aaaattctcc tcttcgaatt agtataattg ttagaaaatt tcaatttaaa aactttactt
    17101 ttgtattaat gactagaaaa caattaatcc agcagattaa gaaaaaaaaa tcctatttat
    17161 gtgttggttt ggataccgat ttagataaaa tcccaaaaca tttattgaaa tacaaacaac
    17221 caattgttga atttaacaag caaattattg aagctacaca agatttatgt gtcgcttaca
    17281 aaccaaacat tgcattctat gaacaatttg gggcaaaagg ttttgagttt ttagaaaaaa
    17341 cattgagcta tattcctgaa aatattttta ctattgccga tgcaaaacgt ggcgacattg
    17401 gcaatacttc aaaaatgtat gcaaaagcat tttttgagac tttaaacttt gattctatta
    17461 ctgttgcacc atatatgggt agcgattcta tcaaaccatt tttagattat aaaaacaaat
    17521 ggacgataat tttaggttta acatcaaata ctggaagtca agattttcaa tttctaaaat
    17581 ccaataaaaa accattatat caacaagtga ttgaaactgc catgcaatac ggaaatgaag
    17641 gcaatacgat gtttgtaatt ggtgcaacac aagaaaaatc tataaaaaaa attcgcacat
    17701 tttgtcctga acatttcttt ttagttcctg gtgttggtgc tcaaggtgga agtttagaaa
    17761 agatttctga atttggttta aatcaagata ttggattgtt ggtaaattcc gctcgcaata
    17821 ttatttatgc atctaatgga aaagattttg ctgaaaaagc tagagatgaa gctcagaaaa
    17881 ttcaacaaga aatgcaagct attctaaaaa gaaaacaatt aatataatta cttgataata
    17941 tgtcagaatt acgatacaat ccattattga atacatatac tattgttgct gcaaatagac
    18001 aaaacagacc caatttccca aaaaattggt gtccattttg tccaggcgaa ggcaaaaaag
    18061 ttccaaataa ttatgaagtt tttgtttatc caaatgattt tccagcattg gattatcaga
    18121 acaaagtaga taacgaaacc actaatgaaa tttacactaa agaagcggca aaaggtgctt
    18181 gtgaagtaat tttatattca cccaatcata ctgcaaattt atacgattta tctgatgctc
    18241 atgttttaaa attgataaaa acttggatag aacgtttcaa ctttcataaa aactttgatg
    18301 atgtaaaata tatctttcca tttgaaaaca gaggtaagga agttggggta acaatgccac
    18361 atccacacgg acaattgtat gcttatccgt ttattcctca aaaaattgaa actgaattaa
    18421 tcaatgccaa aaactacttt agtaaacatc aaataaatct atttgatgca atgctgaaag
    18481 aagaaataga atttcaacaa cgtgttattt ttgaaacaga tacattttta gtttatctac
    18541 cattttttac cgattatcct tatggaattt ttattgtttc taaaaaggaa cgtttataca
    18601 tttccgattt caatgaagag gaacaattgg aactaagtat tgtcttaaaa aaagtggtta
    18661 gaatgtttga caatctattt tcaaaaactt ttccttttat gatgtgtatc catcaaggtg
    18721 tggtcaatag tactgaatat tctgaaaaaa cacaaagaca attttatcgt tttcatattg
    18781 agttttatcc tccacttcgt gatgaaaatg caataaaata ttatgcaagt tcagaaatgg
    18841 gtgcttgggc agctgctaat accaaactgg ttgaagaaac tgcaaaagaa ttaagagccg
    18901 ctttacccca ataaaaaaag gaatgaactt tgttcactcc ttttttagtt attccaaaat
    18961 attccttaat ataatgggaa atcttccata aacttattca ctttcttttt aatatctaaa
    19021 atcaaatctg tatccgcttg attcatcaaa acagtatcta tccaatctac cacttgaaca
    19081 caatcttgtt ccattaaatg tctagttgta atggcagcag ttccaattct aattcctgag
    19141 gttacaaacg gcgatttatc atcaaaaggc accatgtttt tattgactgt aatatctgca
    19201 taacctaatg ctctttcagc atcttttcca ctgatatttt tatttctcaa atcaattaac
    19261 atcaagtggt tgcttgttcc tccagaaata attttataac ctctatctat aaatgcattt
    19321 gccatcgctt gtgcattctt aatgatttgt tccgtataag tattatattc atctgtcaat
    19381 gcttctccaa atgcgactgc ttttccagca ataacatgct ctaaaggtcc accttgcata
    19441 ccaggaaaaa cgccagtatt caaaatctga gacatcattt tcaactcccc ttttggtgtt
    19501 ttttctccaa atggattttc gaaatcttta cccatcataa taataccacc tctaggtcca
    19561 cgaagcgttt tgtgtgtggt tgaagttaca atatgacaat gcggcattgg gtcgttcaat
    19621 ttcttttttg caattaaccc agcaggatga gcaatgtccg ccatcaacaa agcacctaca
    19681 ctatcagcaa tcgctctaaa acgagcataa tcccaatctc tagcatacgc agatgctcca
    19741 caaactatta atttcggttg atgctctttt gctaaagctt cgaccttatc catatcaatc
    19801 aatccagttt cttgctccac gccataaaaa acaggattgt agtgtttgcc tgaaaaattc
    19861 actggagaac catgagataa gtgtccaccg tgagacaaat taaaccctaa aattgtatca
    19921 aatggtttta agcaagctaa aaatacagaa gcattcgctt gagctcctga atgtggctga
    19981 acattggcat attctgcacc aaacaattca cacagtctat caatagccaa ttgctctatt
    20041 tgatctacca cttcacaacc gccataatat ctttttcctg gatatccttc agcatattta
    20101 ttggtcaaac aagaacccat tgcttccatt acttgcttag aagtaaaatt ttcagaagct
    20161 attaattcca atccagttgt ttgtctttct aattcatcat taattagtga gaagattttt
    20221 ttatctcttt ccattttaaa agtatgtttg tgcaaaaata gccatttatt ttgaatactt
    20281 ttcaataaga aaagtcaaat ctacgcacac aaataaaata gtgtttatta agcactacac
    20341 atttaagttc ttaaaaaact cctcgtattt ttcacccaac aaaggaacgg gtttctgttt
    20401 ctcagtaatg gcattcacaa tacccattat ccatagtacc aatagtccta aacttaaaac
    20461 gcctagtata aaaccattat gtacaattct ttcaataata gaaagtgtaa atcctgtcag
    20521 tgttaatcca attgcttgtt tgatatgata ggaagcaaaa gtgctttttt tatttatatt
    20581 tattacaaaa gcaataatca acccaattat tgttaggtaa gctacaactg cagcggtttt
    20641 acccttgtct atatcttcaa aatctgaaat agggttattt tctcctaaaa tttccatatc
    20701 aatatttttt aatacaatat tactaataat taaaaataaa agcaaaaaag tttaaaaatt
    20761 gtagttcttt atatttgtat caacattatt aaaatcacag tcaatcacac tgaatctaaa
    20821 atagtagaaa ttttcggcac ttttcagact ggcagctaag gtctggtagt gtggactatt
    20881 tttcaagttc tttttcaaat tcccccggtg ttaaaatttt cgccgttata aaaggattat
    20941 gttctaataa atctttgtct cctgttacta aataatccgc ttttgaaaag tcaattaagt
    21001 ctaacaaaaa attatctttc gggtctcgat ttataaaatg agtcggctta atatcaactt
    21061 tctcagcaat agtttccaaa agctcaataa gttcttttac attctcttgc ggaaaatact
    21121 ttttaagctt tttacggctt gtaacagttt taatttcagt taaatttgct ctgttgtaat
    21181 tatcgtgatg tttctgtctg aaatatgacg tgtaatttta gtcagccgtt tcccaatcaa
    21241 gaagctaatc caaacattag tgtcaaaaat gactttaatg cttttgtttg gcatagattt
    21301 cctgtcttac tgtttcaact tctgaattta ttgttttcaa gtctaactcc tttgttctaa
    21361 aagattttaa tagccgagtt agcttcgagt ctatagcttc tttttccagt tctttggaaa
    21421 gcttaatttt ttgctgtcga ggtagttgtt ttaccataga cagaatttgg tcaaatgtta
    21481 tatctatttg caatgcggtt ttcatttctc tattttaaaa tcttttttca aatttacgaa
    21541 aaattgttat ttcaaagtat aaattctcga tttcaacgag ttttcgaagt ctgtcagcca
    21601 acgtaaaacg catatctaca atagtagaaa ttatcggcca ttttcagact ttccgcctga
    21661 acttaaaagt acaatatcta caatagtaga aattatcggc cattttcaga cgctgtatta
    21721 atggcggaag aaactacaat ctacaatagt agaaattatc ggccattttc agacattaat
    21781 ggtagaggat ttggcggtta ttcaatctac aatagtagaa attatcggcc attttcagac
    21841 tgataagttg gaagatggta gatacgtatc tacaatagta gaaattatcg gccattttca
    21901 gacatcaaac ccattatttt tgggcttgaa tctacaatag tagaaattat cggccatttt
    21961 cagacgatta ccaaaacaaa gaaactgtta cagatctaca atagtagaaa ttatcggcca
    22021 ttttcagacc aaccattatt agcgagtgta ggagatctac aatagtagaa attatcggcc
    22081 attttcagac taggttttct aactcgtttg ggtatgatct acaatagtag aaattatcgg
    22141 ccattttcag acaagaaaag tgcatatcaa gattatcatc tacaatagta gaaattatcg
    22201 gccattttca gaccataact cgcagattga cgcatgttta tctacaatag tagaaattat
    22261 cggccatttt cagactttat tgattgaaag cttctgcaat caacatatct acaatagtag
    22321 aaattatcgg ccattttcag actttaaaag aaaatcctga ggctaaagat atctacaata
    22381 gtagaaatta tcggccattt tcagaccgcc aagaaaacag gtggcgacat catatctaca
    22441 atagtagaaa ttatcggcca ttttcagacg ttttggaagc caaaaaaggt aataatctac
    22501 aatagtagaa attatcggcc attttcagac atacagctcc cgcaccacag caacaaccat
    22561 c
//
LOCUS       DAOQCW010000028        21847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_870_length_21847_cov_7.031984, whole genome shotgun sequence.
ACCESSION   DAOQCW010000028 DAOQCW010000000
VERSION     DAOQCW010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 21847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21847
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_870_length_21847_cov_7.031984"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2024
                     /locus_tag="PLS16_03995"
     CDS             <1..2024
                     /locus_tag="PLS16_03995"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNJ60275.1"
                     /translation="ASTGICDTTIIDITVTPPPTDTIVDSICKTCPDTLCIDNPFGPG
                     SVTTTTLCDGSTSTSGTVVDVTVDANGCLVFTPRAVVGRDTVCVVVCDSTSGQCDTTI
                     IVVVVPPTVDTIPATVPVDSTVVVCVELEDGMNPSTTTYTYCDGSTGTLNGTLGEAVL
                     GADGCVTYTAGDKAGKEAPICVVACDASTGICDTTIIDITVTPPCDIFNKDSLTVECN
                     GFGKGEYCMPLSLEALANYDLYIDGSLTALTVANASGCGSEVVSAGYGFQTVGYIDNV
                     PHLLEEWSISSDSIVRPNIVFSTLTDLVGYMNSVDASGAWYVDGMNVRPANPSASYRT
                     GTGLVYFALQPEVRTTYISYNYYVGYSSANLLIDQGCHELKVVNKVTGCSDSIVLCVE
                     TCIGDIIYDTIPVKGRDSICTFVTPNNGAITITGCDGSVSGEGNFVDWSINAGCLVYE
                     AGNQKGTDTICIKVCARESEGKEFCYETNVIVTVVGIPPIAVDNDTTTKVNTSVAIPV
                     LGNDIQTDDDLLVLCTIITDPLHGTIQVDYATGIITYTPDNDYRGVDSLQYEICDPDG
                     NDTAWVYIRIVKENECELLNAFSPNGDGVNDTYYIPCVDVSNGDLEFRVFNRWGIEVY
                     HNLKYDNNNGWDGKYKGSPLPDGTYYYVIKYINKDNEQINKAGFIVIQR"
     gene            2102..3115
                     /locus_tag="PLS16_04000"
     CDS             2102..3115
                     /locus_tag="PLS16_04000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNJ60276.1"
                     /translation="MIIKLKQTVQYLVLGILFMFSVSDLYGQQEPQYSQFMYNKLPIN
                     AGYTGSREVLSLRALYRNQWSGITGAPQTATFSIHSPLKKEASALGFYMINDRLGVTN
                     QTYFDASYAYRVPLGKGVKLSIGLNAGLLWFKSNLMSLKIDEQQDLVFQQNVNKVLPD
                     IGAGLYIYHKNAYFGFSVPNFIKGDLQDADVIKKFKNDNSGSFLSAHRTAHFVTMAGG
                     VIPAGKVLFIRPQIMYRYIASAEQKIAHTLDMNLSLLIYNRVNIGASYRTSFHNKSTG
                     LLNGDSFDALIEVWPTKQLLIGFAYDYTLTKLGDYSHGSYDIILGYDFSFEKKRIITP
                     RYF"
     gene            3152..5554
                     /locus_tag="PLS16_04005"
     CDS             3152..5554
                     /locus_tag="PLS16_04005"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNJ60277.1"
                     /translation="MNTLFNNIKLLTLIVFMQSLSMQYVSAQTIEGGGIGANNAIGGI
                     GKAEKLYNELAYAQAIPLYETYLKKNENLKSMLELGDCYRLTSKFDKAEYWYGKAVEK
                     GDVEPEYKLYYAKMLQANEKYEEAAKWYANYKQSVPEDKRATNQLKASADYGQYLLTR
                     DRFQVKNLSFNTSGYDFAPVWYDKGLIFSSSRDSSKAISNESMWTGTQFFDMYYVEGE
                     KTEFGKPKALKADAATKYHEAAATFTPDKKKVYFTRNNFYNNKLKTSSDKIVKLNIYE
                     SDIDGMNWKNDKAFQYNNSEYSVGHPALTADGNSMYFISDMPGGFGGTDIYVTKKEGD
                     SWGIPKNLGEEINTEGMEMFPYVDEEGVLYFASDGQGGLGGLDIFRTRFNEKTEKFGK
                     VRNFGAPVNSSHDDFSLVYGKDKSVGYFTSNRPEGKGLDDIYSFTDDGVYLEGIVVDA
                     ETREPICNSKVIMKAKLTQSEEGKTITECDGEFEFGVIKNMDYCFVASADGYSVNDSV
                     CATTKNVKPGETVFVEIPLYKGKEYAMSIQVLGKSPRNIHDSTLVDSLLPLANAKVLL
                     SSKCEGWTKSLETDISGKICEVVRCDCDYIIVANAKGYLPGNIEAANNPEDCKIDRKC
                     GENPREEQVILDPIPGFGPNGSYSDLYNPDGSYKYGQAILLKDIYYDFDKWHIREESE
                     LELNKLLSFLQENPSTIVEIGSHTDARAPYDYNIKLSQRRAQSVVDWLVARGVDKKRL
                     KPKGYGETQPVNGCVDGVTCTEYEHQRNRRTEFRVIGNNIDLKSLERLDMEVDPCKNC
                     PF"
     gene            5732..6016
                     /locus_tag="PLS16_04010"
     CDS             5732..6016
                     /locus_tag="PLS16_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNJ60278.1"
                     /translation="MAKSKLSIQPLADRVVIKAAEAEQVTKSGIIIPDTAKEKPQRGE
                     VVAVGPGKKDEPTTVKVGDNVLYGKYSGTEINIEGKDYLIMKESDILAIV"
     gene            6070..7704
                     /gene="groL"
                     /locus_tag="PLS16_04015"
     CDS             6070..7704
                     /gene="groL"
                     /locus_tag="PLS16_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456581.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNJ60279.1"
                     /translation="MSAKLINYSTESRDKLKAGVDALANAVKVTLGPKGRNVIIEKKF
                     GAPHITKDGVTVAKEIELEDPFENMGAQLVKDVASKTNDVAGDGTTTATVLAQAIIAP
                     GLKSITAGANPMDLKRGIDKAVAAVVKELKSLSEKVGKDYSKIKQVGTISANNDDEIG
                     GLIAQAMEKVTTEGVITVEEAKGTETYVDVVEGMQFDRGYISPYFVTNADTMECEMEK
                     PYILLYDKKVSSMKDLLPVLEKVVQSGRGLLIIAEDVESEALATLVVNRLRGSLKIAA
                     VKAPGFGDRRKAMLQDIAILTGGEVIAEEIGLKLEDTQITQLGVAEKVVIDKDNTTII
                     NGAGKKTDITARVNQIKSQIETTTSDYDKEKLQERLAKLSGGVAVLYVGAPSEVEMKE
                     KKDRVDDALHATRAAVEEGIVPGGGVALIRCQGALDKLKGANEDEQTGIEIIKVALEA
                     PLRQISANAGVEGSIVVFNVKNGKKDYGYNAKTEVYENLHKAGVIDPTKVTRVALENA
                     ASIASMIISTECAIVEKPSEAPAMPPMPGGGMGGMM"
     regulatory      7783..7980
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            8010..9941
                     /locus_tag="PLS16_04020"
     CDS             8010..9941
                     /locus_tag="PLS16_04020"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNJ60280.1"
                     /translation="MRLKRYIILFIVFANWQHQNLFANQDSIAVQFIDTIIIQDSRFA
                     LYSKSNSNYSIGSDFSKYYTQNSIADLLQYHTPIQILQNGATGVASLNIRGTADDQSN
                     VFWNGVKLNSLSLGSADISLIPINIAQNIEVITNATSTAFGSGNFGAAILLNSKPKFN
                     SGYNVSLRQTFASYKNYKTDFAFTVSNAKIAFSSSSSYQQAENNFKYYDRYKANNPLL
                     RQKHNQLQQWVSVNDLFIKLKKKQTISIGNYTYDKDYQVPSIMGSFENSKKHQDDFGT
                     KTFFNYDRFFRKGKLSLTQNHTYDYLLYTDSTVGLVSKFKTHQAQQTIDYQHQFNSMV
                     LIDVGSSYNFNFAQIKAYKNNKIQHRFAMYAATKFLMKQFNLNLSIRQEVVNADYIRP
                     QFAIAFSFRNKKNFYNTTLSYSDKFRYPDLNDLYWIPGGNINLQPEYGYSTEWQQDFI
                     LRKKQHQLEYANTLYFLRIKNNIVWVPMSGGLSTPLNIKRTQHLGTENTLRYTYALKE
                     YFSIKISSNYNYNRSIILEDKSNTSTDGKIIRYKPEHSIKSTFSMQYKYFDLGLNYLY
                     VSKRYTEDENVEVFALRPYHIIDMYVALKYNHKIFNTALVFKVNNLLNTSYESVRSYA
                     QPLRNYQLSILFNFKSKQS"
     gene            9971..11026
                     /locus_tag="PLS16_04025"
     CDS             9971..11026
                     /locus_tag="PLS16_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60281.1"
                     /translation="MLLVFFVASCQKEKNNDNEGTYIESGSAIVVNEGAFGSNNGSIS
                     FISAEGVVKNNIFELANNGSPLGDVVQSFARVGNKGLICVNNSQKIEVVDVKTFQRLA
                     TINAGDTTNYPRYAVAVASNKAFITNGSGDGIVAVLNLDNYSITKTIPVGDGPEQMEI
                     ASGNVYVCNSGGWGVDSTVSVINIGSETVTKTIKVGDIPTKIIRDAQENLWVLCNGQT
                     DFSAWPIVKNLTPAKLVRINTSTQSVDRVFTLIEANGNTQKLNIAIGNNGTSVYYAID
                     NDIYKLGIDVTALPSSKLFTTSGIYGLGTSYNQQIWVLQAPSFSSSGKVIKYSNAGTA
                     IDSVSVGIGPNGVVFNP"
     gene            11161..13065
                     /gene="dnaK"
                     /locus_tag="PLS16_04030"
     CDS             11161..13065
                     /gene="dnaK"
                     /locus_tag="PLS16_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNJ60282.1"
                     /translation="MGKIIGIDLGTTNSCVSVMEGNEPVVITTEEGRRTLPSIVGFLE
                     NGERKVGDPAKRQAITNPKNTVASIKRFMGKRFSEITKEKSYVAYDVERGDNDTVRVK
                     IYDRLYTPQEISAIILQKAKKMAEDYLGQTVTEAVITVPAYFNDAERQATKEAGLIAG
                     LDVKRIINEPTAAALAYGLDKGGKDMKIAVYDLGGGTFDISILELGDGVFEVKSTNGD
                     VHLGGDDFDHTIIEWLAEEFQNEERMDLRKDPMALQRLKEAAEKAKIELSSSSETEIN
                     LPYITAVDGVPKHLVKKLSKAKFDQLTEALVERTLEPCRKALKDAGYNASDIDEVILV
                     GGSTRIPKIQETVEKFFGKKPSKGVNPDEVVAVGAAIQGGVLTGDVKDVLLLDVTPLS
                     LGIETYGGVFTKLIESNTTIPTKKSEVFSTAGDNQPSVELNVLQGERPMAKDNKSIGR
                     FHLDGIPPAPRGVPQIEVTFDIDANGILSVTAKDKGTGKEQNIRITASSGLSKEEIEK
                     MKQEAKANEAADREAREKVDKLNEADSLIFQTEKQIKEFGDKLPADKKQVIEDALAKL
                     KSAHQAQEVAAIDGALAELNNAWSAASQDLYNATQQGNPGAGEQQQGGDNGNAGTGDT
                     VTDAEYEEVK"
     gene            13227..14225
                     /locus_tag="PLS16_04035"
     CDS             13227..14225
                     /locus_tag="PLS16_04035"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNJ60283.1"
                     /translation="MDSFTQITLGIATAEVIAGKELKNRTFLYGAILGTIPDLDILVG
                     KFMNDVDGVAIHRGLSHSILFFACISPFFAWLIYKMEKGKIQYNKAIWLVFWCLLTHV
                     LLDMFTSWGTQILWPLDYRFALKTIFVVDPLFTIPLLIALIFVWRNKDNFQRQKYLFR
                     GLYISLTYLLLTGCIKLYAVAKFEQALKDNKLIYQHLIVKPTAFNCILWNANVATSEG
                     YYLADYSLFDSQAIRFKFYSKNKTLEEKLTTISDFQKLKSISEGWYLITQQNEKLYFN
                     DLRFGLLNDDEKKPQFAFCYEFIPSSNGNLSAVEVPKTRRDGKALMEKIFTRIKGN"
     gene            14324..15151
                     /locus_tag="PLS16_04040"
     CDS             14324..15151
                     /locus_tag="PLS16_04040"
                     /inference="COORDINATES: protein motif:HMM:NF039623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5995 family protein"
                     /protein_id="HNJ60284.1"
                     /translation="MNRNSFGIFLLVLLICSCKKEQPKTNNQWKLKDADFQTALTLSD
                     TSTLTNLDEAGERLAEIQDIFSFIGDRRGAYPTILKAVTNYAISSFALENYKHTALMR
                     SFYLDFVKRYLGALHEHLLGNKLEYHWDTYYRYAKVDHNVSRLVLEGINAHFSIDMAR
                     AVAQSKLNKSNQVDWDKFGTETLDAVSSFNDELFVEYEVNAEEVFKLFFIGDLVNGLL
                     GEGSATKFGYQMLRNEALSNGILLQLSTNGNVEQNMKVAFTKKDNVILALDKMQMLP"
     gene            15232..16086
                     /locus_tag="PLS16_04045"
     CDS             15232..16086
                     /locus_tag="PLS16_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNJ60285.1"
                     /translation="MSLNEFKGTENYIATKELQVAINAAIALEKPLLIKGEPGTGKTL
                     LAFEVAKALNKPIYTWHIKSTTTAQQGLYEYDAVSRLRDAQFGNEDVKDISNYIVKGK
                     MWQAFESEEQAVLLIDEIDKADIEFPNDLLLELDKMEFYVYELQKTIQAKTRPIVIIT
                     SNNEKELPDAFLRRCFFHFIRFPDKATMIDIIDVHFKNLDNELIDDALKLFYGLRDIQ
                     GLKKKPTTSELIDWIRLLKLGKIDKSELAAVDYIEKQPPYFGSLIKNEQDIDHIGRIK
                     RAGTFRRF"
     gene            16112..17422
                     /locus_tag="PLS16_04050"
     CDS             16112..17422
                     /locus_tag="PLS16_04050"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNJ60286.1"
                     /translation="MEEQDKIPTSKVQRASRFFKTGVKVGGNYMANAAKAVVGKSDAE
                     ELHRKNAEEVFALLSQLKGSALKVAQMMSVDTGLLPKEYVEKFAQAQNNAMSLSGPLI
                     VNTFQKYFDKKPTELYDKFDTKAKFAASIGQVHEAYKDGKKLAVKIQYPGVADAIHSD
                     IQMVKPLAARLIGVPTKFLDYYAEEFEARLIEECDYYLELRNGTEAKIACATIENLII
                     PEYFAALSNAKVLTMEWIDAMSLQEYIHTETDQEKKNQVGQTLVNFIFHQIHHQKRFH
                     ADPHPGNFLVTKNNELVVLDFGCMKTIPADFYKNYFSIADEKIQANPEKLEQLLMQLD
                     VIRPEDTAEERALFYQVAKDAIEIITAPLKSESFYFGDENYTKNLNDKGEQMAKNKEL
                     RKPNALRGSRDAIFLHRTFFGMYGILYQLNATVKMDRHFLDNLI"
     gene            17600..17944
                     /locus_tag="PLS16_04055"
     CDS             17600..17944
                     /locus_tag="PLS16_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60287.1"
                     /translation="MIAQEQLPRSVLYKIVTQTNKFVLNSSTQFAHVDLPSNKYQEFF
                     SFIKDSTANFIPDASNPIYYKWYLNDGSEINGDLFFKENKSYMVFKIDDKKYINYFMP
                     EGLAYLKQIFKF"
     gene            17977..19383
                     /locus_tag="PLS16_04060"
     CDS             17977..19383
                     /locus_tag="PLS16_04060"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNJ60288.1"
                     /translation="MFVNFLVKPIWVFGIDRTVQNQLGETEYGLYFAILNFTYLFQIF
                     LDFGLQNYNARHIASNEKQIGELLPNIILFKLILFIVYIVICMAVAVLLGYINHRFFF
                     MILMNQFLLSFIIYLRSNISAHQHFITDSLLSVLDKLLMITFCAIIIWGNTGFDLSII
                     NFVLAQFAAYLITFLVALFLSFNLITRINFRFSSAFIKQIFRESFPYASIYFLMTIYY
                     RIDGVMLERMKTSYEAGIYAQGYRIMESVNNIGYLMSIILLPIFANYLSKRIDIKTIL
                     MPALRISLILIVCVLGVFYFQAEAIIGILYQADIVYSSKVFSWLVLNFIPISLLYVIG
                     TLLTADEQFRIMLPTLIIAVGLNIVLNYFMIQSIGAIGAAKATFITQLFMLLVYGFAC
                     IVKYSIQLNIKTSIRIILFIAFAYGIMQFLLANYPDLNLKNLIFRSTFYIVAVMIIAV
                     SLGIVDKQILKLNIKPFN"
     gene            complement(19500..19802)
                     /locus_tag="PLS16_04065"
     CDS             complement(19500..19802)
                     /locus_tag="PLS16_04065"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNJ60289.1"
                     /translation="MNYKISIEAKNDIEKIWLYTYENWSLEQADRYFDLIIDEIEYIA
                     KNPASGNDFSDVRNGYLRTRIKSHFIFYKVNKKQNEVEIIRVLHQRMDIENRLNDE"
     gene            complement(19802..20050)
                     /locus_tag="PLS16_04070"
     CDS             complement(19802..20050)
                     /locus_tag="PLS16_04070"
                     /inference="COORDINATES: protein motif:HMM:NF015640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HNJ60290.1"
                     /translation="MAKNTSILLGDYFDNFINQQIKSGKYSSASEVVRTALRILEFEE
                     SKKTELINELKRGEKSGFVKEFNRENFRREIHQKHLAK"
     gene            complement(20759..>21847)
                     /locus_tag="PLS16_04075"
     CDS             complement(20759..>21847)
                     /locus_tag="PLS16_04075"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ60291.1"
                     /translation="GCYVFFDEIQYLKDWERHLKILVDTYPETKFIVSGSAAAALKWH
                     STESGAGRFTDFMLPPLTFQEYIHLKGMNHLIYDGKINYGNNQVPYCLTHDIKAFNKE
                     FIHYLNFGGYPEVVLSERIQSDMGRYVKNDIVDKVLLRDLPSLYGIKDVQELNRFFTY
                     IAYNSGNEFSYERMSQESGIQKDTLKKYLEYLEAAFLIKVLNKVDVNAKRLKRVTSFK
                     VFLTNPSLRTALFSPISEIDKEMGNMVETAVLSQWMHREKLDITYARWKEGRNEGEVD
                     LVLIDDKKFKPVWGVEIKWSNRYFDKPDELKSLITFCKNNKFQKALVTSIDKIGVKEI
                     DNLLFSFLPVSIYSYNIGNITLKMKNNQ"
BASE COUNT         7721 a         2870 c         4029 g         7227 t
ORIGIN      
        1 atgcaagtac aggaatttgt gatacaacaa tcatagatat aaccgtaaca ccaccaccaa
       61 cagatactat cgtagatagc atctgtaaga catgtccaga taccttatgt atagacaatc
      121 catttggacc aggcagtgta acgacaacga cgttatgtga tggaagcact agcacaagtg
      181 gcacagtagt agatgtaaca gtagatgcca atggctgttt agtgtttacc ccaagagcag
      241 tagtaggtag agatacagta tgtgtagtag tttgtgatag tacaagcgga cagtgcgata
      301 caacaattat agtagtagta gtaccaccga cagtagatac tatcccagcg accgtaccag
      361 tagatagtac agtagtagta tgtgtagaat tagaagatgg catgaaccca tcaaccacaa
      421 catataccta ctgtgatgga agcacaggca cattgaatgg cacattaggc gaagcagtac
      481 ttggagcaga tggctgtgta acatatacag caggcgacaa agcaggtaag gaagcaccaa
      541 tctgtgtagt agcctgtgat gcaagtacag gaatttgtga tacaacaatc atagatataa
      601 ccgtaacacc accttgtgat atatttaata aggattcttt aacagttgaa tgtaacggat
      661 ttggtaaagg cgaatattgt atgccattat cattagaagc tttagctaat tatgacttgt
      721 atatagatgg ctctttaact gctcttacag ttgctaatgc atcaggttgt ggctcagaag
      781 tagtttcggc tggttatgga ttccaaactg ttggatatat tgataatgtt ccacatttat
      841 tagaagaatg gagtattagt tcagatagta tagtaagacc taatattgta tttagtacat
      901 taacagattt agtaggttat atgaatagtg ttgatgctag tggagcttgg tatgttgatg
      961 gaatgaatgt tagacctgca aatccttctg catcttatag aacaggaact gggttagtat
     1021 attttgcatt acaaccagaa gttagaacaa cttatatttc ttataattat tatgttggat
     1081 atagttctgc taatttattg attgatcaag gttgtcatga gcttaaagtt gtcaataagg
     1141 ttactggttg ttcagatagt atagtacttt gtgttgaaac ttgtatagga gatattatct
     1201 atgatacaat accagttaaa ggaagagata gtatttgtac ttttgtaaca cctaataatg
     1261 gagcgattac tattactgga tgtgatggat cagtttctgg agaaggtaat tttgtagatt
     1321 ggtcaattaa tgcaggctgt ctagtttatg aagcaggaaa tcagaaagga acagatacga
     1381 tatgtattaa agtttgtgca agagaatctg aaggtaaaga attctgttat gaaactaatg
     1441 ttatagttac agtagttgga attcctccaa ttgcagtaga taatgataca acaacgaaag
     1501 tgaatacttc tgtagcaata ccagtattag gaaatgatat ccaaacagat gacgatctat
     1561 tagtattgtg tacgataatt acagatccac ttcatggaac aattcaggtt gattatgcga
     1621 caggtataat tacttatact ccagataatg attacagagg agttgatagt ttacaatatg
     1681 aaatatgcga tccagatggt aatgatactg cttgggtgta tatacgaatt gtgaaagaaa
     1741 atgaatgtga attattgaat gcattctctc cgaatggaga tggtgtgaat gatacttatt
     1801 atataccttg cgtagatgta agtaatggag accttgagtt tagagtattt aaccgttggg
     1861 gaattgaggt ttatcataat ctgaaatatg ataataataa tggatgggat ggtaagtaca
     1921 aaggttcacc tttaccagat ggaacttatt actatgtaat taaatacatt aataaggata
     1981 acgaacaaat taataaagca ggttttatag taattcaacg ataaaatgga aggagtgtta
     2041 ctaaaaagag taacactcca tttcattaat aaattaaaaa gagcattaaa agataattct
     2101 tatgataata aagctaaaac aaacagttca gtatctagta ttaggcattt tgtttatgtt
     2161 tagtgtgtct gatttatatg gacaacaaga accacaatat tcgcagttta tgtataataa
     2221 attgccaatc aatgcaggat atactggatc aagagaggtt ttgagtttga gagcattgta
     2281 tagaaatcaa tggtcaggta taacaggagc tccgcagacg gcaacattct ctatacatag
     2341 tcctttgaaa aaggaagctt ctgccttagg attttatatg attaatgacc gtttaggtgt
     2401 aaccaatcag acatattttg atgcgtcgta tgcttataga gttccattgg gaaaaggcgt
     2461 aaaattaagt ataggattaa atgcaggtct attatggttt aaaagtaatt taatgagttt
     2521 aaaaatagac gaacaacagg atctggtttt tcaacaaaat gtaaataagg tactaccaga
     2581 tattggtgct ggattatata tttatcataa gaatgcatat ttcgggttta gtgtacctaa
     2641 ctttatcaaa ggggatttac aagatgcaga tgttattaag aagtttaaga atgataattc
     2701 aggtagtttc ttgagtgcac atcgtacagc acactttgtt actatggctg gaggtgttat
     2761 ccctgctgga aaagtattgt ttatacgccc tcaaattatg tatagataca tagcaagtgc
     2821 agaacaaaaa attgctcata ctttagatat gaatttaagt ttattaattt ataatagagt
     2881 aaatattggg gcaagttata gaacatcatt ccataataaa agcactggat tattgaatgg
     2941 agatagtttt gatgccttaa tcgaagtatg gccaaccaag caattattaa ttggttttgc
     3001 ttatgattat actttaacga aattaggaga ttatagccat ggtagttatg atattatatt
     3061 gggttatgat ttctcatttg aaaagaaacg tattataacc cctagatatt tctaattcag
     3121 agattaataa gaagaaaaat agataaaaaa tatgaataca ttgtttaata atataaagct
     3181 acttacttta attgttttta tgcaatcatt aagtatgcaa tatgtatcag cacaaacaat
     3241 tgaaggtggt ggaattggag ctaataatgc tattggtgga attggaaaag ctgaaaaact
     3301 atataacgaa ttggcttatg cacaagcaat cccattatat gaaacttatt taaaaaagaa
     3361 tgagaatcta aaatcaatgt tggaattagg tgattgttat cgcttaactt ctaaatttga
     3421 taaggctgaa tattggtatg gaaaggcggt agagaaagga gatgtagaac ctgaatataa
     3481 gttgtactat gcaaagatgt tgcaagcgaa tgagaaatat gaagaagcag ctaaatggta
     3541 tgccaactat aaacaaagtg ttcctgaaga taaaagagca actaatcaac ttaaagcgag
     3601 tgctgattat gggcaatact tattaaccag agatagattt caagtaaaga atttatcatt
     3661 taatacttct ggttatgatt ttgctccagt ttggtatgat aaaggattga ttttctcctc
     3721 ttcacgtgat agctcaaaag caattagcaa tgaaagtatg tggactggaa ctcaattttt
     3781 tgacatgtat tatgttgaag gagaaaaaac ggaatttggt aaaccaaaag ctttaaaagc
     3841 cgatgctgca acaaaatatc atgaggccgc agcgacattt acaccagata agaagaaagt
     3901 atattttact agaaataatt tttacaataa taaactaaaa acgagttcag acaaaatagt
     3961 aaaattaaat atttatgagt ctgatataga tggtatgaat tggaaaaatg acaaggcttt
     4021 ccaatataat aatagtgagt atagtgttgg gcatccagca ttaactgctg atggtaattc
     4081 gatgtacttt atcagtgata tgcctggagg atttggtggt actgatattt atgtaactaa
     4141 aaaagaagga gatagttggg gaatacctaa aaacttagga gaggaaataa atactgaagg
     4201 aatggaaatg tttccttatg ttgatgaaga aggtgttttg tattttgcta gtgatggtca
     4261 aggcggttta ggtggtttgg atatttttag aacaagattt aatgaaaaaa cagaaaaatt
     4321 tggtaaagta agaaattttg gagctccagt taatagctct catgatgact tttcattagt
     4381 ttatgggaaa gataaatcag taggatactt tacatcgaat agaccagaag gaaaaggttt
     4441 agatgatata tatagcttta ctgatgatgg tgtttattta gaaggtattg tagtcgatgc
     4501 agaaactaga gagcccatct gtaatagtaa agttattatg aaagcaaaac tcactcaaag
     4561 tgaagaagga aagactatta cagaatgtga tggagaattt gagtttggtg taattaaaaa
     4621 tatggattat tgttttgttg cgagtgctga tgggtatagt gtaaatgata gtgtttgtgc
     4681 aacaacaaaa aatgtaaaac ctggagaaac tgtatttgtt gaaattcctt tatataaagg
     4741 taaagaatat gctatgagta ttcaagtttt aggaaagtct ccaagaaata ttcatgattc
     4801 tactttagta gatagcttat tacctttagc aaatgctaag gttctattgt caagtaaatg
     4861 tgagggttgg acaaaatcat tagaaacaga tattagtggt aaaatttgtg aagttgtaag
     4921 atgtgactgt gattatatta ttgtcgcaaa tgcaaaaggt tatttacctg gaaatattga
     4981 agcagctaat aatcctgaag attgcaaaat tgacagaaaa tgtggagaaa atccaagaga
     5041 ggagcaggtt attttagacc caataccagg atttggacct aatggttcat acagtgattt
     5101 atataatcct gatggttctt ataagtatgg tcaagcaatt ttattgaaag atatttatta
     5161 tgatttcgat aaatggcata ttagagaaga gagtgaatta gaattaaata agttattaag
     5221 tttcttacaa gaaaacccaa gtacaattgt agaaattggt tctcatacag atgctagagc
     5281 cccttatgat tacaatatca aattgtctca aagaagagct caaagtgttg ttgattggtt
     5341 agtagctaga ggcgtagata agaaacgttt gaaacctaaa ggttatggcg aaacacagcc
     5401 agtaaatgga tgtgtagatg gtgtcacttg tactgagtat gaacatcaga gaaacagaag
     5461 aactgagttt agagtaattg gaaataatat agatttaaaa tcgttagaac gtttagatat
     5521 ggaagtagat ccatgtaaaa attgtccatt ctaataaaga aaaaatatat ttaaaattag
     5581 gaaataggct accgaatggt agcctattta tttagtatga taaattgacc aagtttaatg
     5641 acagccaatt tgtcatttat gttggtttgg catattatta gcatagattt agcaaaatca
     5701 aaattattaa ctaaataaat taaaatattt tatggcaaaa tcaaaattaa gtattcaacc
     5761 attagccgat agggttgtaa tcaaagctgc agaagcagaa caagtaacaa aatctggtat
     5821 tattattcct gacacagcaa aagaaaaacc acaaagaggc gaagttgttg cagttggtcc
     5881 aggaaaaaaa gatgagccaa ctacagttaa agttggtgat aatgtattat acggcaaata
     5941 tagtggaact gaaatcaata tagaaggaaa agattattta attatgaaag aatctgacat
     6001 attggctatt gtttaatagc cttttgtcat ttgaatagag tataaacaaa aaaacaaata
     6061 taaaatatta tgagtgcaaa attaattaat tatagtacag aatccagaga taagttaaaa
     6121 gctggagttg atgcattagc aaatgcagtt aaagtaacct taggaccaaa aggaagaaat
     6181 gttattattg agaagaaatt tggagctcct cacattacaa aagatggagt tacagttgct
     6241 aaagaaattg aattagaaga tccatttgaa aatatgggtg ctcaattagt gaaagatgtt
     6301 gcttcaaaaa ctaatgatgt tgcaggagac ggtacaacaa cagctactgt attagctcaa
     6361 gcgattattg ctccaggttt aaaatcaatt actgcaggag ctaatcctat ggatttaaaa
     6421 agaggaattg ataaagcagt tgctgcggtt gtaaaagaat taaaatcact ttctgagaaa
     6481 gtaggtaaag attatagcaa aatcaaacaa gtaggaacga tttcagcaaa taatgatgat
     6541 gaaattggtg ggttaattgc tcaagcaatg gaaaaagtta caacagaagg tgtaattact
     6601 gttgaagaag ctaaaggaac tgaaacttat gttgatgttg tggaaggtat gcaatttgac
     6661 agaggttata tctcaccata ttttgtaacc aatgcagata caatggagtg tgaaatggag
     6721 aaaccttata ttttattata tgataaaaag gtatcttcta tgaaagattt attgcctgta
     6781 ttagagaaag tagtacaatc tggaagagga ttattaatta tagcagaaga tgtagaaagt
     6841 gaagctttag caactttagt agttaataga ttgagaggtt ctttgaaaat tgcagcagta
     6901 aaagctcctg gttttggaga tagaagaaaa gcgatgttgc aagatattgc gatattaact
     6961 ggtggcgaag taattgcaga agaaattgga ttaaaattag aagatacaca aattacacaa
     7021 ttaggcgttg cagagaaagt agtaatagat aaggacaata cgactattat taatggtgct
     7081 ggaaagaaaa cagatattac tgctagagta aatcaaatta aatctcaaat tgaaacaaca
     7141 acttctgatt acgataaaga gaaattgcaa gaacgtttag caaaattatc tggaggtgtg
     7201 gctgtattgt atgttggagc tccatctgaa gtagaaatga aagaaaagaa agatagagtt
     7261 gatgatgcat tacatgcaac acgtgcagct gttgaagaag gaatcgttcc tggtggtggt
     7321 gtcgctttaa tcagatgtca aggtgcatta gataaattaa aaggagcaaa cgaagacgag
     7381 caaacaggaa tagaaattat taaagtagca ttagaagctc ctttgcgtca aatttctgca
     7441 aatgctggtg tagaaggttc tattgtcgta tttaatgtaa aaaatggcaa aaaagattac
     7501 ggttacaatg caaaaactga agtttacgaa aacttacata aagcaggcgt tattgaccca
     7561 actaaagtaa ccagagtagc attagaaaat gcagcatcaa ttgcaagtat gataattagt
     7621 acagaatgtg caatagttga aaaaccaagt gaagctccag caatgccacc aatgccaggt
     7681 ggtggaatgg gcggaatgat gtaataattt attattgaac agatttttat aaggcactat
     7741 tttttagtgc ctttttttat tgtgataatc ctaaattttt atacctttgc aaaagatttt
     7801 ggtttttata catcaaagta aaaattaaaa gggaaacaag tgagagtctt gtacagtccc
     7861 agctgctgta agcacgctta atgaaaatta agcatcgaaa acaaacacca ctgagaattt
     7921 tcttgggaag gtttcgttaa agtgcgagtc agaagacctg ccaaagtcag ttaaaataat
     7981 taaaatgttc tgaggaaaca ttctaagaga tgagactaaa aaggtatatc atattattta
     8041 tagtatttgc taattggcaa catcaaaatc tatttgcaaa ccaagattca attgccgttc
     8101 aattcattga tacaatcatt attcaagatt ctagatttgc actatattca aaatcaaata
     8161 gtaattactc gattggtagt gatttttcaa aatattacac ccaaaattct attgcagact
     8221 tattgcaata tcatacgcct atacaaattt tacaaaatgg ggcgactgga gttgcttcgt
     8281 taaatatcag aggaacagcg gacgaccaaa gtaatgtttt ttggaatgga gtaaaactta
     8341 attccttgtc tttaggttct gccgatatct ctttaattcc aataaatatt gctcaaaata
     8401 ttgaagttat tacaaatgca acttcaacag cttttggttc aggtaatttt ggtgcagcaa
     8461 tattattgaa ctcaaaacca aagtttaatt caggttataa tgtttcctta cgtcaaactt
     8521 ttgcttccta taaaaattat aaaacagatt tcgcattcac ggttagtaat gctaaaattg
     8581 cattttcctc ttcctcttcc tatcagcaag cagaaaataa tttcaaatat tatgatagat
     8641 ataaagccaa taatcctttg ctccgacaaa aacacaatca actacaacaa tgggtttctg
     8701 tcaatgattt atttattaag ttaaagaaaa agcaaacgat tagtatagga aattatactt
     8761 atgataagga ttatcaagtt ccatcaatta tgggaagttt tgaaaactct aaaaaacatc
     8821 aagatgattt cggaacgaag acttttttta attatgatag attttttaga aaaggaaagt
     8881 tgagcctaac tcaaaatcat acgtatgatt atttattata tacagatagc accgtcggct
     8941 tagtgtctaa atttaaaacc catcaggcac aacaaaccat tgattaccaa catcaattca
     9001 attcaatggt tttgattgat gttgggtcat cttacaattt caattttgca cagattaagg
     9061 catataaaaa caataaaatt caacatcgtt ttgcaatgta tgcggccact aagttcttga
     9121 tgaaacaatt taatttgaat ttgtcaataa gacaagaagt agtgaatgct gattatatcc
     9181 gtcctcaatt tgcaatcgct tttagcttta gaaataaaaa gaatttttac aatactacgc
     9241 ttagttattc tgataaattt agatacccag atttgaatga tttgtattgg atacctggag
     9301 gaaatattaa tttacagcca gagtatggtt attcaacaga atggcaacaa gattttattt
     9361 taagaaaaaa gcagcatcaa ttggaatatg ctaatacttt atattttctt agaattaaaa
     9421 ataatattgt ttgggtgcca atgagcggag gattatctac acctttaaac atcaaaagaa
     9481 cgcaacactt aggaacagaa aatacgctaa gatatactta cgcattaaag gaatactttt
     9541 caataaaaat aagctccaat tacaattata atcgttcaat tattttggaa gataaatcta
     9601 atacatctac tgatggaaaa attattagat acaaaccaga gcattccatc aaatctactt
     9661 tttcaatgca gtataaatac tttgacttag gcttaaatta tttgtacgtc agcaaaagat
     9721 atacagaaga tgagaatgta gaagtttttg cacttcgacc ttatcatatt atagatatgt
     9781 atgtagcact aaaatataat cataaaatat ttaatacggc tttagtattc aaggtaaata
     9841 atttactaaa cacatcttat gaaagcgtta ggagttatgc tcagccactt cggaattatc
     9901 aattatcaat actatttaat tttaaatcaa aacaatcatg atcaaacaat tcaacatcaa
     9961 aatgatggca atgttactgg tattttttgt agcaagttgc caaaaagaaa aaaacaatga
    10021 caacgaagga acttatatcg aatcaggttc ggcaattgtt gtaaatgaag gagcttttgg
    10081 tagcaacaat ggttctattt catttattag tgcagaaggc gtagtgaaaa ataatatttt
    10141 tgaattagca aacaatggaa gtccactggg agatgtcgtt caatcttttg ctagagttgg
    10201 gaataaagga ttaatttgtg tcaataattc acaaaaaatt gaagtcgtag atgttaaaac
    10261 atttcaaaga ttagccacaa taaatgctgg agatactact aattatccac gatatgcggt
    10321 agctgttgct tctaataaag cttttattac gaatggttct ggagatggaa tcgttgctgt
    10381 tcttaaccta gataattata gcattactaa aaccattcct gttggagatg gtcctgaaca
    10441 aatggaaatc gcatcaggaa atgtttatgt atgtaatagt ggtggttggg gcgttgatag
    10501 tactgtttca gtaatcaata taggatcaga aactgtaaca aaaactatta aagtaggaga
    10561 tattccaaca aaaataatta gagatgcaca agaaaactta tgggtacttt gtaatggaca
    10621 aacggatttt tctgcttggc ctattgtgaa gaatttaacg cctgcaaaat tagtaagaat
    10681 taatactagt acgcaatcag tagatagagt ttttactctg attgaagcaa atggtaatac
    10741 acaaaaatta aatatagcaa ttggaaataa tggcacatcc gtttattatg caatagataa
    10801 tgatatttat aaattaggaa ttgatgtaac agcacttcca agcagtaaac tatttacaac
    10861 aagtgggatt tatggtctag gtacttctta taatcaacag atttgggtat tgcaagcacc
    10921 tagtttttct tcttcaggaa aggttattaa atactcaaat gcaggaacag caattgactc
    10981 ggtaagcgtt ggtattggac cgaatggtgt agtttttaat ccataatatt agacaaaaaa
    11041 tcatcaaata gacacagagc catcattaaa ttgatggctc ttgtcatttt gtcataaaaa
    11101 taccccttgg cacaagtatt gttcttatcc atataaataa taaaaagaaa ataattaaat
    11161 atgggaaaaa taataggaat tgacttagga actaccaact cttgtgtatc cgtaatggag
    11221 ggaaatgagc cagtagttat tacaacagaa gaaggaagaa gaaccttacc ttctatcgta
    11281 ggatttttgg aaaatggtga gcgtaaagtg ggcgaccctg caaagcgtca agcaatcacg
    11341 aatccaaaga atacagtagc ttcgattaag cgttttatgg gtaaacgttt ttctgagatt
    11401 actaaagaaa aatcgtatgt cgcttatgat gtagaacgtg gcgataatga tactgtgcgt
    11461 gtaaaaatct atgacagatt atatacacca caagaaattt ctgctatcat tttacaaaaa
    11521 gcaaaaaaaa tggcagaaga ctatcttgga caaaccgtta ctgaagctgt aattacagtt
    11581 ccagcttatt ttaacgatgc agaaagacaa gcaacaaaag aagcaggatt aattgcaggt
    11641 ttagatgtaa aacgtatcat caacgaacca actgctgcag cattagctta tggtttagat
    11701 aaaggtggaa aagatatgaa aatcgctgtt tatgacttag gtggcggtac ttttgatatc
    11761 tctatcttag aattaggtga tggtgtattt gaagtaaaat ctactaatgg agatgtgcat
    11821 ttaggtggtg atgactttga ccatactatt attgaatggt tggctgaaga atttcaaaat
    11881 gaagaaagaa tggatttaag aaaagatcca atggcacttc aaagattaaa agaagcagcg
    11941 gagaaagcta aaatagaatt atcttcttct tcagaaactg aaattaactt gccttatatc
    12001 actgcagtcg atggcgttcc aaaacactta gtgaaaaaat tatcaaaagc aaaatttgac
    12061 caattgactg aggctttagt agaaagaact ttagagcctt gtagaaaagc attgaaagat
    12121 gcaggttata atgcttcaga tattgacgag gtaattttag taggtggttc tacaagaatc
    12181 cctaaaattc aagaaactgt agagaaattc tttggtaaaa aaccttcaaa aggtgtaaat
    12241 ccggatgaag tagttgctgt aggtgctgca attcaaggag gtgttttaac aggagatgta
    12301 aaagatgttt tattgttaga cgttacaccg ttgtctttag gaattgaaac ttatggtgga
    12361 gtatttacaa aattaattga atcaaatact acaatcccaa ctaaaaaatc tgaagtattt
    12421 tcaactgcag gagataatca accatctgta gaattgaatg tattacaagg agaacgtcca
    12481 atggcaaaag ataataaatc tattggtcgt ttccatttag atggtattcc accagcacca
    12541 cgtggcgttc ctcaaattga agtaactttt gatattgatg ctaatggtat cttgagtgta
    12601 actgcgaaag ataaaggtac tggaaaagag caaaatatta gaattacagc ttcttcaggt
    12661 ttgagcaaag aagaaatcga aaagatgaaa caagaagcta aagctaacga agctgctgat
    12721 agagaagcta gagaaaaagt agataaattg aatgaagcgg atagcttaat tttccaaact
    12781 gaaaagcaaa ttaaagagtt tggcgataaa ttaccagctg ataaaaaaca ggtgattgaa
    12841 gatgcattag ctaaattaaa atctgcacat caagctcaag aagttgctgc gatagatgga
    12901 gcattagcag agttaaataa tgcttggagt gcagcttctc aagatttata taatgcaaca
    12961 caacaaggaa atcctggagc aggagagcaa caacaaggcg gagataacgg aaatgctgga
    13021 acaggagata cagttactga tgcagagtat gaagaagtga agtaagtaca ttttaaaatc
    13081 gtttataaaa aaggagtaag ttttgcttac tcctttttgt attttagcat agtgaatttt
    13141 ttatatatga agatgtttgt attgaatata agaaatatga aggattaatt aacaatgctt
    13201 atttttgttt attattatag aaattattgg actcatttac tcaaataaca ttaggaattg
    13261 ctactgcaga ggtaattgct ggcaaagagt tgaaaaatag aacttttttg tatggtgcaa
    13321 ttttgggaac aattcctgac ttggatattc tcgttggtaa atttatgaat gatgttgatg
    13381 gtgtcgctat tcatcgtggg ttgagccatt ctattttgtt tttcgcttgt atatcgccat
    13441 tttttgcatg gttgatttat aaaatggaga aaggaaaaat tcaatacaat aaagcaattt
    13501 ggttggtgtt ttggtgttta ttgactcatg ttttattgga tatgtttaca tcttggggaa
    13561 cacaaatttt atggccatta gattatagat ttgctttgaa aacaattttt gtggttgatc
    13621 cattgtttac aattccatta ttgattgcat tgatttttgt ttggagaaac aaagataatt
    13681 ttcaaagaca gaagtatctt tttagagggt tgtatattag tttgacttat ttgttgctta
    13741 caggttgtat aaagttgtat gccgttgcta agtttgaaca agcattgaaa gataataagc
    13801 taatttatca gcatttaatt gtgaaaccaa ccgcttttaa ttgtatttta tggaatgcaa
    13861 atgttgcaac gagcgagggt tattatttag cagattattc attgtttgat agtcaagcga
    13921 tacgttttaa attctattca aaaaataaaa cattagaaga aaagctgact acaatttcag
    13981 actttcaaaa attaaaaagc ataagtgaag gttggtattt gataacacaa caaaatgaaa
    14041 aattatattt taatgacttg cggtttggct tattaaatga tgatgaaaaa aagccacaat
    14101 ttgcattttg ttatgaattt atacctagtt caaatggaaa tctttccgcc gttgaagttc
    14161 ccaaaacaag acgagatggt aaagcattaa tggaaaaaat ttttacaaga ataaaaggta
    14221 attgacaaat attagtgcgt tgggataaat aaaatatacc caaaagtcgg cacaataaaa
    14281 ttatggattc tacgtatatt gcagtcataa ataatatttt tttatgaatc gaaattcctt
    14341 tggaatattt ttattagtct tattgatttg ttcttgtaaa aaagaacagc ctaaaaccaa
    14401 taaccaatgg aaattaaaag atgctgattt tcaaacagca cttacattat cagatacatc
    14461 aacgctaact aatttagatg aagctggaga acgattagct gaaatacaag atatcttttc
    14521 ttttattgga gacagacgtg gggcatatcc tacgatttta aaagcagtta caaattatgc
    14581 aattagctct tttgctcttg aaaattataa gcatacggca ttaatgagga gcttttattt
    14641 ggattttgtg aagcgttatt taggtgcttt acatgaacat cttttaggca ataaattaga
    14701 ataccattgg gatacttatt atcgttatgc aaaggtagat cataatgtca gtcgcttagt
    14761 tttggaagga ataaatgctc atttttctat tgatatggct cgtgctgttg cacagtcaaa
    14821 actcaataaa agcaatcaag tagattggga taaatttggc acagaaacct tagatgcagt
    14881 atctagtttt aatgatgaat tatttgtaga atatgaagta aacgccgaag aggtttttaa
    14941 attatttttt attggcgatt tagtaaatgg cttgcttggc gaaggtagtg cgactaaatt
    15001 tggttatcaa atgcttagaa atgaagcttt gagcaatggt attcttttgc aattatctac
    15061 aaatggaaat gtggagcaaa atatgaaagt tgcatttacc aaaaaagaca atgttatttt
    15121 ggctttggat aaaatgcaaa tgttgcctta gtagaagcta aaaaatctaa aaatttctat
    15181 accaattttg ttaggctttg tacccattta tttttgtagt atcttagcat tatgagtctg
    15241 aatgaattta aaggaacgga aaattatatt gcaaccaaag aattacaagt tgcaataaat
    15301 gctgcaattg cattagaaaa gccacttttg ataaaaggag agccaggaac tggtaaaact
    15361 ttattagcat ttgaagtcgc aaaagcactc aataaaccaa tttatacttg gcatattaaa
    15421 tcgacgacta cggcacaaca aggattatat gaatatgatg cggtttctcg tttacgtgat
    15481 gctcaatttg ggaatgaaga tgtaaaagat atttctaatt atatagtcaa gggaaaaatg
    15541 tggcaagctt ttgaaagtga agagcaagcg gttttattga tagatgaaat tgataaagca
    15601 gatattgaat ttccaaatga tttgctctta gaattagata aaatggagtt ttatgtttat
    15661 gaattacaaa aaactattca ggcaaaaact cgaccaatcg tgattattac ttctaataat
    15721 gaaaaggaac taccagatgc gtttttaaga agatgttttt tccattttat aagattccca
    15781 gataaagcaa cgatgattga tattattgat gtacatttta aaaatttgga taatgaattg
    15841 attgatgatg cattgaaatt attttatgga ttgcgagata ttcaaggctt aaagaaaaag
    15901 ccaacaacga gtgaattaat agattggatt cgtttattaa aattaggaaa aatagataaa
    15961 tcagaattgg cagcagtaga ttatatagaa aagcagccac catattttgg ttctctaata
    16021 aaaaatgaac aagatattga ccatattggc agaataaaaa gagcaggaac atttagaaga
    16081 ttctaggcat aaagattgac gtaaatttga gatggaagaa caagataaaa taccaactag
    16141 caaagtgcag cgtgcaagcc gattttttaa aacgggtgta aaagttggtg gtaattatat
    16201 ggcaaatgca gccaaagctg tagttggaaa atctgatgct gaggaattgc atcgtaaaaa
    16261 tgcggaagaa gtttttgcct tgttgagtca gttgaaagga agtgctttaa aagtggcaca
    16321 aatgatgagt gtagatacag gtttgttgcc aaaagaatat gtagaaaagt ttgctcaagc
    16381 tcagaataat gcaatgtcat tatcgggtcc tttgattgta aatacttttc aaaaatattt
    16441 tgacaagaaa ccaacagaac tctatgataa atttgataca aaagccaaat ttgcggcatc
    16501 tatcgggcaa gtacacgaag cctataaaga tggtaaaaag ttagccgtaa agattcaata
    16561 tccaggcgtg gcagatgcca ttcattctga tattcagatg gtaaaaccat tggcagcacg
    16621 cttgattggc gtacctacta agtttttaga ttattatgct gaagaatttg aagctcgatt
    16681 gatagaagaa tgtgattact atttggaatt aagaaatgga acagaagcaa aaatagcttg
    16741 tgcaacgatt gaaaatttaa ttattcctga atattttgca gcactttcaa atgccaaagt
    16801 attaacgatg gaatggattg atgcaatgtc tttacaagaa tacattcata cagaaactga
    16861 ccaagaaaag aaaaatcaag ttggacaaac tttagtgaat tttatttttc atcaaatcca
    16921 tcatcaaaaa agatttcatg cagatccaca tccaggcaat tttttggtta ccaaaaataa
    16981 tgaattggtt gttttagatt ttggttgcat gaaaactatt cctgccgatt tttataagaa
    17041 ttatttttct attgcagatg agaaaataca agcaaatcca gaaaaactag aacaattgtt
    17101 gatgcaatta gatgtcattc gaccagaaga tacagctgaa gaaagagcct tgttttatca
    17161 agttgccaaa gatgcaattg aaattattac ggcaccttta aaatcagaat ctttttattt
    17221 tggagatgag aattatacta aaaatttgaa tgacaaagga gagcagatgg cgaaaaacaa
    17281 agaattaaga aaaccaaatg ccttgcgagg tagtcgagat gcgatttttt tacaccgcac
    17341 ttttttcggt atgtatggta ttttgtatca attaaatgcg actgtaaaaa tggacagaca
    17401 ttttttggat aatttgatat agattttaga taaaggatat tttaatctaa aatatgcttt
    17461 attgaaaaaa cgctatgcta ttattgtttc aaaattgaaa taataggata ctttctttat
    17521 atttggtata attttagcta tttagttatg tatgaaatca ttgatattta ttgtttttag
    17581 cttgtttttt tcttataaaa tgattgcaca agagcaattg ccacgtagtg ttttgtataa
    17641 aattgttaca caaaccaata agtttgtttt gaatagtagt acacaatttg ctcacgttga
    17701 tttaccttca aataaatatc aagagttttt tagtttcata aaagatagta cggcaaattt
    17761 tattcctgat gcttcaaatc ctatttatta taaatggtat ttgaatgatg gttcggaaat
    17821 taatggcgat ttgtttttta aggaaaataa aagctatatg gtctttaaga ttgatgataa
    17881 aaagtatatc aattatttta tgcctgaagg tttagcctat ttaaaacaaa ttttcaagtt
    17941 ttgaaaaaat ccttactgtt caatatcgga tttttggtgt ttgtcaattt tttggttaag
    18001 ccaatttggg tatttggaat agatagaacg gtacaaaatc aattaggaga aacagaatat
    18061 ggcttatatt ttgccattct gaattttaca tatttatttc agatattttt ggattttgga
    18121 ttgcaaaatt acaatgccag acacattgca tctaatgaaa aacaaatagg agaattattg
    18181 cccaatatca ttttattcaa actgatttta tttatagtgt atatcgtgat ttgtatggcg
    18241 gttgctgttc ttttaggata catcaatcat cgatttttct ttatgatttt gatgaaccaa
    18301 tttttacttt cgtttattat ctatttgcgt tctaatattt ctgcacatca acattttata
    18361 accgatagct tgttgtctgt tttagataaa ttattaatga taactttttg tgctatcatc
    18421 atttggggaa atacaggatt tgatttaagc atcataaatt ttgttttggc acaatttgct
    18481 gcctatttga ttacttttct tgtggcattg tttttatctt ttaatttaat tactagaatc
    18541 aattttagat tttcaagtgc ctttataaaa caaattttta gagaaagttt cccttatgca
    18601 agtatctatt tcctaatgac catttattat cgcattgatg gtgtgatgtt ggaaagaatg
    18661 aaaaccagtt acgaagctgg aatttatgct caaggctata gaattatgga atctgttaat
    18721 aatattgggt atctgatgag catcatttta ttaccgattt ttgcaaatta tttgtctaaa
    18781 agaatagata ttaaaacgat tttgatgcct gctttgcgaa ttagtttgat acttattgtt
    18841 tgtgtattag gtgtattcta ttttcaagca gaagcaatta ttggtatttt atatcaagca
    18901 gatattgttt attcgtctaa agttttttcg tggttggttt taaatttcat tccaatcagc
    18961 ttattgtatg ttattggaac tttacttact gctgatgagc aatttagaat aatgctacct
    19021 actctaatta ttgcagtggg tttaaatatt gttttaaatt attttatgat acaaagtatt
    19081 ggtgcgattg gtgcagcaaa agcaactttt ataacacaac tatttatgtt gttggtatat
    19141 ggatttgctt gtattgtcaa atattctatc caactaaata taaaaactag tatcagaatt
    19201 attcttttta ttgcatttgc gtatggaatc atgcaatttt tattagctaa ttatccagac
    19261 ttaaatctaa aaaacctaat ctttagaagt actttttata ttgttgctgt tatgattatt
    19321 gcggtttctt tagggattgt tgataagcaa attttaaaat tgaatattaa accattcaac
    19381 taaatcattt ttttatttct aatttttatt gagaattaag gtttattaga agtacatagg
    19441 ccgaattttg tacttcgtct cagtttttga aaagctgctc gttatagttg ccataatttt
    19501 tactcgtcat ttagcctatt ttctatatcc attctttgat gaagcactct gataatttcc
    19561 acttcattct gtttcttgtt cactttgtag aaaatgaaat gagattttat tcgagttctc
    19621 aaatatccat ttctcacatc actaaaatca tttccagaag cagggttttt agcaatgtat
    19681 tcaatttcgt ctattatcaa atcaaaatat cggtcagctt gttcaagcga ccaattttca
    19741 taagtgtata gccaaatctt ttcaatatca tttttcgctt caatacttat tttgtaattc
    19801 attatttagc gagatgtttt tgatgaattt ctcttcggaa gttttctctg ttgaattctt
    19861 tcacaaagcc tgatttttcg cctcttttca attcatttat caattccgtt tttttagatt
    19921 cttcaaattc taaaattctt aaagctgttc gcacaacttc acttgccgaa gaatattttc
    19981 cacttttgat ttgttgattt ataaaattgt caaaataatc acccaataaa atcgaagtat
    20041 tttttgccat aatttttaat ttggtttaaa tataccaatt cttggtatag aatcaaaact
    20101 ttttactggc tttctgcttt agaatggttg tagataaagt ttagcagctt tgcgagttag
    20161 caatttactc tctgtttgtg tttaagccgt ctattgtagg tagtgtattt taagtcgtta
    20221 ttgtatagtg tgaagccttg tcaaatgatt gtttaagttt agagtaaaca gtatcatttt
    20281 tgcctcttac ttgcccaaca aagttgatat atccttcgag gtgattgatg aatttgcaac
    20341 actgtttcat agagtagttg tttcaactga aatacaattt ctttttcagc agcattggag
    20401 ttattgctta ttatattttc aattttattt ttcaagtcct gtttcattct ataaaaatac
    20461 ctttttgtgt cggtttattt tcgttgttaa aaaaatactt ttcaataatt atatttaatg
    20521 atatagtgaa gaatgattat tcaaataagt ttccttgatg aaaatttgtt ccaaatatag
    20581 tagaaattct aaaagaggct cttcgaaacg ctttgggtcg cttctattta tttgagaagg
    20641 taatgtatga tttcgttaaa acaatcctca tcgtattcat ttttactttt ctgacattct
    20701 cctcctattt aggattttct tctattcgta atgttgtaca cttgttgcta tatatttgtt
    20761 attgattatt tttcattttt agagttatgt taccaatatt ataactgtag atagatactg
    20821 gtaagaatga aaataataaa ttatctattt ctttaacacc tattttgtct attgatgtaa
    20881 ctaaagcttt ttgaaattta ttgtttttgc aaaatgttat caaacttttt agttcatctg
    20941 gtttgtcaaa ataacggttg ctccatttta tctccacacc ccaaactggc ttaaattttt
    21001 tgtcatcaat taaaactaaa tcaacttctc cctcatttcg tccttctttc caacgagcat
    21061 aagttatgtc tagtttttca cgatgcatcc attgggataa aacagcagtt tctaccatat
    21121 ttcccatttc tttgtcgatt tcacttattg gtgaaaacaa agctgttcgt aatgatggat
    21181 tggttaaaaa aactttgaaa cttgttactc gttttaaacg tttagcattt acatcaactt
    21241 tattcaatac cttaattaga aatgccgctt ctaaatattc taaatatttt tttagcgtgt
    21301 ctttttggat accactttct tggctcattc tttcataaga aaattcgttg ccagagttgt
    21361 aggctatata tgtgaaaaat ctgtttaatt cttgtacgtc ctttattcca tacaaacttg
    21421 gtaaatctct tagtaaaact ttgtcaacta tatcgttttt tacatatctt cccatatcgc
    21481 tttgtattct ttcacttaat actacttcag gataacctcc gaagtttaga tagtggataa
    21541 actccttatt gaaagctttt atatcgtgtg taagacaata tggtacttga ttgttgccat
    21601 aatttatttt tccatcataa ataagatgat tcattccttt gagatgaatg tattcttgaa
    21661 atgtaagagg aggcaacata aagtcggtaa atctgccagc cccactttcc gtactatgcc
    21721 attttaatgc tgcagcagca gaccccgata caataaattt agtttctgga taagtgtcaa
    21781 caagtatctt tagatgtctc tcccaatctt taagatattg tatttcatca aagaaaacat
    21841 aacatcc
//
LOCUS       DAOQCW010000029        21806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_872_length_21806_cov_5.064687, whole genome shotgun sequence.
ACCESSION   DAOQCW010000029 DAOQCW010000000
VERSION     DAOQCW010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 21806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21806
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_872_length_21806_cov_5.064687"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="PLS16_04080"
     CDS             <1..843
                     /locus_tag="PLS16_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNJ60292.1"
                     /translation="LTQQLNSTLNNLNLNKNNLEKDIDDLTNERNKVNEQLDKTLSNL
                     NQNKTLLEKDINQLNQQKESLTDEKKQLQAENDKIAKEKAKLEADKVSMDQLLAQMQE
                     ERDRLAKEKEKMENDKLLLEKLRKQQEKEVMALSQNIDSLKKAQQKAIDANASLQRYE
                     IFNVPIEEGAVAIMKSIYFMADASFIQNKSYPELDKLVAFLKQNPKIQKIEIGGHTNG
                     LCDDVFCNKLSNDRAKSVVEYLINKGINANILSYKGYGKQYLISKPGDAINQRVEVKI
                     LSVQ"
     gene            complement(850..1512)
                     /locus_tag="PLS16_04085"
     CDS             complement(850..1512)
                     /locus_tag="PLS16_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNJ60293.1"
                     /translation="MYLIDSCKIIKNNLNFRIKNKYTNFEVIMSLALKTFIANYATFR
                     PDELEQIVSSFKHKKFKKNEFVLTKGSVCQDLVFVEKGCLRLYYIQDDIETSVWFAMQ
                     NSSAIEMHSFISETPTNYYIQAIEDSEIAYLPKIELTILYQKYPVLQEMMRKFWEDVI
                     INLLERFTALQRDSAEKRYLDLLNKPDYLQNIPQKYLASFIGVTPTSLSRIKKQIMLQ
                     GQ"
     gene            1502..2224
                     /locus_tag="PLS16_04090"
     CDS             1502..2224
                     /locus_tag="PLS16_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60294.1"
                     /translation="MRYILLLCLLCSTKFGFLKAQQNDTLNIKHKFKPEKVKFVVGIN
                     YTAFVPLMQQKDHFIGVGKINDSTHAFVDRNSKGNLNKNYSASNMQMSVQFNFWKNLY
                     NGLHYEFFTVKNYKRHGKNLLSKKNTMFFAIATSVGYSFEFLKKKNLQLMPTFRIGGY
                     AADSYFDGLGKKIYLGLDFKMRYFIKNKFGFSAGIDYEYFRFKAKGYNNNYQKPTYQK
                     TSINNIFFNLGIAYNIQIKLDK"
     gene            2232..3362
                     /locus_tag="PLS16_04095"
     CDS             2232..3362
                     /locus_tag="PLS16_04095"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNJ60295.1"
                     /translation="MAVRKKIPTLKIITVIIVLTLSIITFKLNDSLRVPSIFDGKFYS
                     TLNIPKAKQPKILPEKISSIPELQVWADSATYNQYLYTDILLQKSNTTSFLVVKDGKV
                     IFKRYLNGVKEGDITQVFSVTKVFVTALLGMAIQDKIIEDINLPVSYFYKELKNTKFD
                     SVTLLHLCQMTSGLNYDEYGNLLQTIRFYYSDNLHSVIENATFSDVPGKKFMYKSIDT
                     QILGECITKALGKKTFMEYFYEKIWNPLGMQDSAFFTVDNKITRTPKFYGGLNITARD
                     LAKFGIMVANNGIYNKKRILPKDWFNQCDNTSFRSDSDDKYCLGWYYMVDDDSSDVYF
                     AAGFNGQTMMINETTNVVVIRLGIDRGNINWYPIMKKLSESV"
     gene            3439..4170
                     /locus_tag="PLS16_04100"
     CDS             3439..4170
                     /locus_tag="PLS16_04100"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNJ60296.1"
                     /translation="MKKLSLLFLICLSFIAFGKGKKKKTETPTPTPTNTVSENPLKTK
                     LDSVGYILGLQIAEDISKNTVDRPSLEGIKLGFSDAYNKNPYKIPEGKYQEIVQKFFQ
                     EQQNALLVAKGAQNKAFLETNGKKEGVNTTSSGLQYQVIKLGDGAKPKATDVVTVYYN
                     GTLVDGTHFDGNIGEEPISFPLNQVILGWTEGLQLMPVGSKFKFYLPPHLAYGEQGSP
                     PVIGPNEVLIFDVELLGIKDATAPK"
     gene            4195..4929
                     /locus_tag="PLS16_04105"
     CDS             4195..4929
                     /locus_tag="PLS16_04105"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNJ60297.1"
                     /translation="MKKQLFYLAAIALLFTACNKGSKAYQSAKPKTEIDSVSYAIGVQ
                     IASDLQRGGIDKSINPELVARAIYDVMNEKSKLFGGDTVAAVLMKYFDPQSFSKLEEN
                     KKKEATFFSENGKKANVKTTASGLQYEIISEGTGAKPKETNTVKVHYKGELLDGTVFD
                     SSIDRKEPAIFPLANLIQGWKEGILLMPVGSKFKFYVPSNLAYGSQGIPQAGIGPNEP
                     LVFEVELISIEPDAPTTMQIDPSTIK"
     gene            5144..5788
                     /locus_tag="PLS16_04110"
     CDS             5144..5788
                     /locus_tag="PLS16_04110"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNJ60298.1"
                     /translation="MALSFLKFFQPKDKIFHKLFEDSIVIGAQISEIFVEAMSETGAK
                     RFEILAKTSNLEHQADDIARQIFSELNRNFITPFDREDIYALASALDDVIDYIDEVGS
                     KIKNYEFGESNEYILKMAEYNNQSVKELKNAIYGLKSMKELEKVTDACIKVHGYESIV
                     DTIYNQAMGVLIKNNKDNPIKVIVLKDLYEEMELISDKCQDASNVISSIVIKYS"
     gene            5837..7504
                     /locus_tag="PLS16_04115"
     CDS             5837..7504
                     /locus_tag="PLS16_04115"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNJ60299.1"
                     /translation="MEFIVVIILLALIFDYINGFHDAANSIATIVSTKVLTPLQAVIW
                     AAFFNMAAFFIFQDHAVANTIGKTVYKDFITLPVIFSGLVAAIIWNLLTWWYGIPSSS
                     SHTLIGGFAGAAVTHAFIDKGGWVPIREIIDSGKILSIIAFIFLAPVIGMAISIFYTI
                     IMMNKNTWIKVAALTVTCIITWNVFIHFQEKKMNSNMAHFFKLDKYEKAINDPRLIDD
                     LEKNKEKETIAKAELAKVAPYIHLYNEKGVDFVANEIVNNIDLDRVTKLHIEDNLKIF
                     FKVEQLKILAQKDSTEKQSYQSAVDAVDALKPHIGLYKELSPDAYAKAMMAKVAIQPN
                     KIKEFKQVLNYDIKSDISNEISRADNSIIRFGLIGMLIIIMLSFIYNEKIKEPSAQRT
                     ANNFKNLQLLSSATFSIGHGGNDAQKVMGIIAAALVANGSITDIKNMPDWVPLSCYLA
                     ISIGTLSGGWKIIKTMGSKITKVTPLEGVCAETAGATTLFLTEQMGIPVSTTHTITGA
                     IIGVGATKRLSAVRWGVTINLLWAWILTIPISALIAAITYGIVNIFM"
     gene            complement(7548..8558)
                     /gene="murB"
                     /locus_tag="PLS16_04120"
     CDS             complement(7548..8558)
                     /gene="murB"
                     /locus_tag="PLS16_04120"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF000755.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNJ60300.1"
                     /translation="METLTNFSLKPYNTFGLEVFAHSFCIVKTKEDIQLLIVNGLLKE
                     QPFLLLGGGSNILFTKNFEGLVIKNEIEGINIVQETDSEVWITAQSGTIWHDLVLFCV
                     QNNYQGIENLALIPGTVGAAPIQNIGAYGVEVKDVLENIFVTDLSTGEEKTISASDCA
                     FGYRDSKFKQEWKNKYFISNITIKLKKKDFNFNTSYGNIQDYLGDKPVSVESIANAVI
                     AIRTSKLPNPKELGNAGSFFKNPEINPSLFKSLQTKYPEIPYYIINDNSVKIPAAWLI
                     EQCGFKGKVIGNTGNHAKQSLVVVNYGEATGTEIWEHAQNVIQSVKVKFNIVLSPEVN
                     VL"
     gene            complement(8558..9844)
                     /locus_tag="PLS16_04125"
     CDS             complement(8558..9844)
                     /locus_tag="PLS16_04125"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNJ60301.1"
                     /translation="MKRELTIFTGSFVIILMSFYMIASGFAQGRPIDNKHKKFVKPVV
                     KMQEKKCIDSIKTEFLGIDIAKFYVEEHQVGRRESLSKIIGRLNYSKKEIKTLTKQLD
                     GIDEFESIKAKQKYFTFSSIKDSVSTVDFIAFELDQKDYLIIDCNNKTAISIYAKPID
                     TVQQQLAFVVNNKVHKILKAKNINVALIDKINQAFNNKISVGQLRTGDTLRVLFSEYF
                     INGTYYETGAIQSAEVITKKKNYFITNYQPEGTKTQQYLDEKGTYTHMTFLKSPLKNG
                     RLTSRFNLSRFHPVLMEVKAHLGTDFAAPKGTPILATADGIVEVAGSTSNNGNFVKIK
                     HDKVYETQYLHMSKIEKGMKKGTRVKQGQVIGYVGSTGLATGPHVCYRFWKNGKQVDP
                     LKEKLNVSKSIPKNQMPQFEQVVQAQKAKLHNILIG"
     gene            complement(9851..10165)
                     /locus_tag="PLS16_04130"
     CDS             complement(9851..10165)
                     /locus_tag="PLS16_04130"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNJ60302.1"
                     /translation="MKLIDPELKEKIPSIFKSKVFYIVVAYLVYLTFFNQYNLLTQFS
                     LFKELRQLSKEEKFYEKSIVEIKEQQADIFKDRNSIEKFAREKYWMKKDSEEIYIFVP
                     KQ"
     gene            complement(10214..11695)
                     /locus_tag="PLS16_04135"
     CDS             complement(10214..11695)
                     /locus_tag="PLS16_04135"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HNJ60303.1"
                     /translation="MSSKSHQLSKSTLIRSIQCLKSLYLYKNHYNLRDIPDRKQQQKF
                     DRGHRVGKLAQQLFPGGKDCTPPSPMHYQQSIAATKLLIENNYPIIYEAAFKFQGILV
                     ALDILECKDGNFYAYEVKSSMSVSQTYIQDCAIQYYVIQHFGIHFADFQIVHINNNYV
                     KNGALEVDRLFKRKSVLKEIVELQPYIQEKIDAAIQVLSKTQMPEIAIGAHCSKPYPC
                     DFMSYCWKDIPANSIWFLQGVSLNDKLEAVNTGILTIDEYAQKHQDDIKLHSYLTNKE
                     IIQKENIANYFKNVQYPICFFDVEAFQSAIPIFDGTKPYERIPFLYSLHIQDEVNATL
                     IHKDYFSATDGDYRKTFIKQFLEDTKTCKTILVYNDLIEKGVLYYLANLFPEFKPEID
                     KRIAIMLDVEIPFKRLDYYHPKQLGSLSLKTISATMLDINPYNAVDVKDGQEAMAIYN
                     ELFYKSKEEQELYYDKLRSYCKTDTYVLSEVYKKLLKIANLGF"
     gene            11751..12887
                     /locus_tag="PLS16_04140"
     CDS             11751..12887
                     /locus_tag="PLS16_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60304.1"
                     /translation="MKKNTFLVLILLTGLSTFAQKGFQVGGNFTTGLSYMLAQNNYFL
                     APNVQMNGANSSKELDYKAKFSYNTGLFFGYNFAEKHGIRVLAGYSIEGQKYQDIFKW
                     PIYGLQGQHTKEVNFQFVNTNVSYRFAPILKGQKEKNPNGNYPNGQYKIRMRLLVGLE
                     VDALVNAKMKYKIERLTEEQLRDENWQGWYDPNPAPDFSEYPYIKPGNPFGLPPAYPA
                     VQPYGNGGYAPYYALGKPKSQKDYFVPLQAGLTINYGFDYIFKNNMYFGVGLDAKVGL
                     NDINSKPYRTHPEYKKSKNYFVGLKVEIGYNILKDADKTPKKKTSSSSKIKTEKAPKE
                     KAEDQVKTPESVSQSKEIGEKAYTKYNKYGQKKDVKIKRLKKKK"
     gene            complement(12944..13396)
                     /locus_tag="PLS16_04145"
     CDS             complement(12944..13396)
                     /locus_tag="PLS16_04145"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNJ60305.1"
                     /translation="MNAQKVRVTKKFTFDMAHALYGYDGPCKNIHGHTYHLSVTLIGT
                     PIQNNADVKLGMVIDFGELKGIVKKQIIDIYDHALVLNEEAPYSKSDVITNEFEKVIL
                     VPFQPTCENLLLHFVHSIQSLFPENIQLISIRLEETPTSFAEWFKQDN"
     gene            complement(13456..14202)
                     /locus_tag="PLS16_04150"
     CDS             complement(13456..14202)
                     /locus_tag="PLS16_04150"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNJ60306.1"
                     /translation="MTDFGLIETIRVSNGYAMFLNDHTNRLIQSLQLLKINIDSETIF
                     RKISNYIQQECKIQDSQNIRLRIQVKINETRIVEWLVSSQKLYTSTFEINKIPLKIGF
                     YDETYKEQNIHSNLKSTDRLIYDKAKKFALENSFDDVLVFNSEKVLSDAIIYNVFIVK
                     ENQIYTSRIKDAPIDGVLRQFLLKHIRSFTIIEKALYKEEIEQADEIFLTNAVRGIQT
                     VGQIGDKKLDNRITLNIYKEFISLLDKTMN"
     gene            complement(14262..>14852)
                     /locus_tag="PLS16_04155"
     CDS             complement(14262..>14852)
                     /locus_tag="PLS16_04155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNJ60307.1"
                     /translation="YFCTIKLNNMTNLKIITSTSRTVGNGKYIAKWITELAQQSGLYN
                     VELLDLAEINLPFMDEANHPMAQKYELEHTKAWSKKINEADAFIFVLAEYNFGIPATI
                     KNAMDYLHFEWKYKPVGIVSYGGISAGLRSAQMFKQVVTSFNMMPIVEQVAIPFFFKS
                     LDNEGKFNADEMVQKSANVMLNELKRWSDALAVLRK"
     assembly_gap    14853..14952
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14953..16793)
                     /locus_tag="PLS16_04160"
     CDS             complement(<14953..16793)
                     /locus_tag="PLS16_04160"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNJ60308.1"
                     /translation="MNQLQYETSPYLLQHKNNPVNWYAWNDVALEKAQKEQKMILVSV
                     GYSACHWCHVMEHESFEDEEVAKVMNQYFVAIKVDREERPDIDQIYMNAAQMTSGQGG
                     WPLNVVCLPDGKPIYGATYFPKERWLQFLHHFQNLFETDKEKMLAQAERLQKGIQLVD
                     TVHFNTDVREIDERFYHQIWKNWKDKLDYEEGGTLGMPKFAMPIALEFLLRYQSRTKD
                     EKLSAYLKTTLKKMAFGGIHDIVAGGFARYSVDAYWKVPHFEKMLYDNAQLLTVYAQA
                     YQITKRPMYKSVCYKILDWINEEMTDKSGGIYAALDADSEGVEGKFYCWNYDEFIQIM
                     AHINHQNCKHIDIKNFGIELYNITKEGNFEHGMNILFRTKEHEYFCEKYQLEQKVFHE
                     LVDEINARLAKAREHKIRPHLDDKILTEWNALAIIGLMNAYKAFGDQLFLNKAISIAN
                     FIIDKVLQSDNRLYRSFKNDKVSINGFLADYVFFIECLILLFQSTFDEQYLKIAQQLT
                     AYCIQHFYSAEHKMFYITSNLDTALVVRSMDSSDNVIPAANSQMAKCLLYLSKYLDNH
                     EYEHIAVAMLNNVLDSVVKNGQYYANWAIVLDMYLYQNIELVIVGKNA"
     gene            17006..18979
                     /locus_tag="PLS16_04165"
     CDS             17006..18979
                     /locus_tag="PLS16_04165"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNJ60309.1"
                     /translation="MINKITKLLVANRGEIAIRIFKTCKEMGISTVAVFSDADENSLF
                     VKYADEAVRIGGKQPNESYLLIDKIIDAAKQTGANAIHPGYGFLSEKENFAQRCIDEN
                     IIWVGPNPNAIEKMGSKIGAKTIMQANNVPTIPGYQGEDQSEATIKAKAIEIGFPVLL
                     KASAGGGGKGMRIVRQEKELDKAINEAKSEAMNAFGDSTLLIEKYFDTSRHVEFQIFG
                     DKHGNAIHLFERECSIQRRYQKVVEESPSPFITEDTRKKMGAAAILACRAIQYDNAGT
                     VEFIVAPDQSFYFLEVNTRLQVEHPITEEVTGLDLVRMQIEVAEGQALQVKQEALQQI
                     GHAIEVRLYAEDPANNFLPVSGKIIDWIPSETDGLRYDTGIESGSEISTFYDPMIAKI
                     IAKGNNRQEAINKLNLALKNTVVTGFTTNKNFLSAILENQDFKAGNFNTHFLDKVFNY
                     SGENYPQTTLDILTCALQHYRFLKRNDKRSIAKGVPAGWRNNFYQAQNEKYAYQGFDF
                     IVEYVNKNEYLEISFADKTFISKFISNHDKVYIFEMNNIRRAFYFSHNGNQYFINAPQ
                     LGQIQLNIVDRFPLPIEEVVKGGYIAPMPGEVTNVLVKTGDIVKAGDALLKMISMKME
                     NTIEAHTDGEIEEIYVTDKQFVESGTLLLKMKA"
     gene            19613..21457
                     /locus_tag="PLS16_04170"
     CDS             19613..21457
                     /locus_tag="PLS16_04170"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing HNH endonuclease family
                     protein"
                     /protein_id="HNJ60310.1"
                     /translation="MEKKTLSNILEGKIFQIPDYQRGYAWEEKQWKDFVQDIDALIDD
                     KIISHYTGTIVIYQPSKKPTENYGTKRLEIVDVVDGQQRLTTCSLYLSIIIKELIKIS
                     QEDFNAEIPIYLYSGSKSKLRLNNDTADFYLDLISKGVSNIEANSVHQKRIYDAYCFL
                     KKHIEEQLTKRVDKGEDYLRDLFDAIIRKLNFSFYPIEVESEIGMTFELMNSRGKNLS
                     SMELLKNYLMYWVYRNILDNSEKEDFTSKINKTWKEVYVNIAKCNGSESQCLRIAWTL
                     FVNYTPKNWNGYSGFKSDEVIPLRNFNSKSKEDVKEFLLKFVDGLALISKHYSAIIKP
                     NNNSFDENELNLLTKIRNAGNIANYLPLMVASRIKKETNEVTKDDYFDLLKSLEIFSY
                     RVFLWEGKRSNAGLSKFYRWADDVFSSKHSLKSVTDWIYGTINWYSPENGFRKSLTED
                     FFGWYHYRRLLKYTLFEYELFLLKGKNKPKLNWEDLTDSTIEHILPQNPDSNSNWLIK
                     WTDEERKKYLHDISNLVLTKDNSRYSNFEFDRKKGIAGSGFSYSNSDIRQERKIAEYD
                     DWTPDTCKKRREELTNWVIKNWGIDKHYQEPIVDVIEEDDEPIFNDVD"
BASE COUNT         7881 a         3279 c         3210 g         7336 t
ORIGIN      
        1 ttaactcaac aattgaatag tacgctcaat aatttaaact taaataaaaa taatttagaa
       61 aaagacatag atgatttaac gaatgaaaga aataaagtga atgaacaatt agataaaaca
      121 ttaagtaatt taaatcaaaa taaaacgctt ttagaaaagg acatcaatca gctcaaccaa
      181 caaaaagaat cacttacaga tgagaaaaag caattacaag cagaaaatga taagattgca
      241 aaagaaaaag caaaattaga agctgataaa gtttctatgg atcaattatt agcacagatg
      301 caagaagaaa gagaccgttt ggctaaggaa aaagagaaaa tggaaaatga caaactacta
      361 cttgaaaaac ttcgtaaaca acaagaaaaa gaagtcatgg ctttatctca aaatattgat
      421 tcgcttaaaa aggcacaaca aaaagccata gatgcaaatg ctagtttaca acgttatgag
      481 atttttaatg ttccaattga agaaggtgca gttgcaatta tgaaaagtat ttattttatg
      541 gctgatgctt catttataca aaacaaatca tatcctgaat tagataaatt ggttgccttt
      601 ttaaagcaaa atccaaaaat ccaaaaaata gaaataggtg gacacaccaa tggactttgc
      661 gatgatgtat tttgcaataa attatccaat gatagagcaa aatctgttgt agaatattta
      721 attaataaag gcataaatgc aaatatatta tcctataaag gttatggtaa acaatatctt
      781 atttctaaac ctggcgatgc tatcaatcaa agagtagaag taaaaatatt gagtgtgcaa
      841 taaataaaat tattgacctt gtaacataat ttgttttttt atacgactta aagacgttgg
      901 agttacacca ataaatgagg ctagatattt ttgaggtata ttttgtaaat aatctggttt
      961 attgagtaaa tccaaatatc ttttttcagc actatcacgt tgcagagccg taaatctttc
     1021 taataaattg ataatgacat cttcccaaaa cttacgcatc atttcttgta aaacaggata
     1081 tttttggtat aaaatggtca attcaatttt aggcaaataa gcaatttcag aatcttcaat
     1141 agcctgaata taataattgg tgggtgtttc gctgataaag ctatgcattt ctattgctga
     1201 tgaattttgc atagcaaacc aaacagaagt ttcaatatcg tcttgaatat aatataatct
     1261 caagcaacct ttttcaacaa atactaaatc ttggcacaca ctacctttcg taagtacaaa
     1321 ttcgtttttt ttaaactttt tatgtttaaa gctggacaca atttgctcca attcatctgg
     1381 tcgaaatgtc gcataattag caataaaagt ctttaaagcc aaactcataa ttacctcaaa
     1441 attagtgtat ttatttttaa ttctaaaatt cagattgttt tttataattt tacaactatc
     1501 tatgagatac atattgctcc tatgtttact ttgcagtacc aaattcggat tcctcaaagc
     1561 ccaacaaaac gatacattaa atattaaaca taaattcaaa cctgagaaag taaaatttgt
     1621 agtcggtatc aattacacag catttgttcc attaatgcaa caaaaagacc attttattgg
     1681 agttggtaaa ataaatgact caacccatgc ttttgttgat agaaattcca aaggaaatct
     1741 aaataaaaat tattccgcta gcaatatgca aatgagtgtt cagtttaact tttggaaaaa
     1801 tttgtataat ggtttgcatt atgaattttt tactgtcaaa aattacaaac gacatggcaa
     1861 aaacttatta tctaagaaaa ataccatgtt ttttgccatt gccacatcag taggttattc
     1921 atttgagttt ttaaaaaaga agaatttaca attaatgcct acttttagaa ttggtggtta
     1981 tgctgccgat agctactttg atggcttagg aaaaaaaatc tatttaggat tagattttaa
     2041 aatgaggtat tttataaaaa ataagtttgg tttttcagct ggtatagatt acgaatattt
     2101 tagattcaaa gcaaaaggct acaacaacaa ttatcaaaaa ccaacttatc agaaaacttc
     2161 catcaacaat attttcttta atctaggaat tgcctataat atccaaatta aactagacaa
     2221 ataaaggcta aatggcagtt cgaaaaaaaa taccaacctt aaaaatcatt actgtaatca
     2281 ttgttcttac gcttagtata attaccttta agttaaacga ttcgctacga gttccaagta
     2341 tttttgatgg gaaattttat agtacattaa atattccaaa agcaaaacaa cccaaaatat
     2401 tacctgagaa aattagttcg attcccgaac tacaagtctg ggctgattca gctacctata
     2461 accaatattt atatacagat atacttttgc aaaagtctaa cacaacttca tttcttgttg
     2521 ttaaagatgg aaaagtcatt tttaaaagat atttgaatgg tgtaaaagaa ggtgatatta
     2581 ctcaagtatt ttcagttact aaagtttttg ttactgctct tttaggcatg gctatccaag
     2641 acaaaattat agaagacatt aatcttccag tatcctactt ttataaagaa ttaaaaaata
     2701 caaaatttga tagtgttaca ctattacatc tatgtcaaat gacttctggt ttaaattatg
     2761 atgaatacgg aaacttactt caaaccatac gtttttatta tagcgacaat ttgcattctg
     2821 ttatagaaaa tgcaacattt agtgatgtgc ctggtaagaa atttatgtat aaatccatag
     2881 acactcaaat attaggcgaa tgcattacaa aagctttggg caaaaaaact tttatggaat
     2941 atttttatga aaaaatatgg aacccattag gtatgcaaga ttctgctttt tttacagtcg
     3001 ataataaaat tacaagaact cctaagtttt atggtggatt aaatattact gcaagagatt
     3061 tagcaaaatt tggtataatg gttgcaaata atggaatcta taacaagaaa agaatattac
     3121 ctaaagattg gttcaatcaa tgcgacaata cctcatttag aagtgattct gatgataaat
     3181 attgtttagg ttggtattat atggtagatg atgacagtag tgatgtttat tttgcagctg
     3241 gtttcaatgg tcaaactatg atgataaatg aaacaacaaa tgttgttgtt attcgattgg
     3301 gaatagatag aggaaatatc aattggtatc caattatgaa aaaactttca gaatctgtct
     3361 agatttattt taataaaaaa ggttaataca tcaaataatt caaatactta gcttactttt
     3421 gcacaataaa tttttaatat gaaaaaacta tctttactat ttttgatttg cctatccttt
     3481 atagcatttg ggaaaggtaa aaaaaagaaa actgaaactc caactcccac accaacaaat
     3541 acagtttctg aaaatccatt aaaaacaaaa ctagactcag taggttatat tttaggactt
     3601 caaattgctg aagatattag caaaaacacc gtagatagac catcattaga aggtatcaaa
     3661 ttaggtttca gtgatgctta caacaaaaat ccttataaaa tacctgaagg taaatatcaa
     3721 gaaatcgttc agaaattttt tcaagaacaa caaaatgcac tattagttgc aaaaggtgct
     3781 caaaataaag catttttaga aactaatggt aaaaaagaag gtgtcaatac aacaagttct
     3841 ggcttacaat atcaagttat aaaattgggt gatggtgcta aaccaaaagc taccgatgtt
     3901 gtaactgttt actataatgg aactttagtt gatggaacac attttgacgg aaatattgga
     3961 gaagagccaa tttcatttcc tttaaatcaa gtaattttag gctggacaga aggtttgcaa
     4021 ttaatgccag ttggctctaa atttaagttt tatttgccac cacatttagc ttatggagaa
     4081 caaggaagcc caccagttat cggaccaaac gaagtgttaa tttttgatgt ggaattatta
     4141 ggtataaaag atgccacagc accaaaataa aatataatta ttaaatacaa accaatgaag
     4201 aaacaattat tttatctagc agcaattgcc ttattattta ctgcttgtaa caaaggttct
     4261 aaagcttacc aaagtgcaaa acccaaaaca gaaatcgatt cagtatctta tgctatcggt
     4321 gtacaaattg catcagactt acaaagaggt ggcattgata aatcaataaa tcctgaacta
     4381 gtagctagag caatttatga tgtaatgaat gaaaaaagta aattatttgg tggtgatact
     4441 gttgccgctg tattaatgaa atattttgac ccacaatcat tttctaaatt agaagagaat
     4501 aagaaaaaag aagcaacatt ctttagcgag aatggcaaaa aagcaaatgt aaaaacaact
     4561 gctagtggct tacaatacga aataatcagt gaaggcacag gtgcaaaacc taaagaaacc
     4621 aatactgtga aagttcatta taaaggtgaa ttactagacg gaacagtttt cgatagttca
     4681 atcgacagaa aagaaccggc aatatttcct ttagcaaatt taatccaagg ttggaaagaa
     4741 gggattttat taatgccagt aggttctaaa tttaagtttt atgttccatc aaatttagct
     4801 tatggttctc aaggaattcc acaagctggt attggaccaa atgaaccttt agtgtttgaa
     4861 gtagaattaa tttcaattga gcctgatgct cctacaacta tgcaaataga tccttcaaca
     4921 ataaaataat aacaattttt agtttaataa aaatgcagtt ctttttgaac tgcatttttt
     4981 gtataaagta atttcaaatc taaaattgta tctatacatt atatattttt ttagattaaa
     5041 tttttgaaaa ttattacaca taaaatatat acacaattaa tctaaaaaaa agacaaatag
     5101 caaaactaat attgtaaatt tgttccaaca aaatatttaa aatatggcac tttcattttt
     5161 aaaattcttc caacctaaag acaaaatttt ccataaacta tttgaagatt caattgtaat
     5221 aggtgcacaa attagtgaaa ttttcgttga ggctatgagt gaaactggtg ctaaacgttt
     5281 tgagatatta gcaaaaactt caaatttaga acatcaagct gatgatattg cacgtcaaat
     5341 attttctgaa ctcaatagaa atttcattac accatttgac agagaggata tttatgcact
     5401 agcttcagca ttagatgatg ttattgatta tattgatgaa gtaggaagta aaattaaaaa
     5461 ttacgaattt ggagaatcta atgaatacat tttaaaaatg gcagagtata ataatcaatc
     5521 cgttaaggaa ttaaaaaatg ctatttatgg tttaaagagt atgaaagagt tagagaaagt
     5581 tacagatgct tgtattaagg ttcatggtta cgaatctatt gtggatacca tttacaatca
     5641 agccatgggt gttttaatca aaaataataa agataatcca atcaaagtca ttgtacttaa
     5701 agatttatat gaggaaatgg aattaatttc tgacaagtgc caagatgcct ctaatgtaat
     5761 ttcttctatt gtaataaagt atagctaaat caaagtttaa attgaacttt tcttttgaaa
     5821 aattaaaaaa ataagaatgg agtttattgt agtaattata ttattggctt taatttttga
     5881 ttatatcaac ggctttcatg atgcagcaaa ctctattgca accattgtat ctactaaagt
     5941 tttaacacct ctacaagcag taatctgggc agcatttttc aatatggctg ctttttttat
     6001 ttttcaagac catgcagtag ctaatacaat aggcaaaacg gtttataagg attttattac
     6061 gcttccagta attttttcag gcttagttgc tgctattatt tggaatttat tgacttggtg
     6121 gtatggtatc ccttcttcat cttcacatac attaatcggt ggatttgcag gtgctgcagt
     6181 tacacatgcc tttatcgaca aaggtggttg ggtacccatt cgtgaaatta ttgatagtgg
     6241 aaaaatttta agtataattg catttatctt cttagctcca gtcattggta tggctatttc
     6301 tattttttat accatcataa tgatgaataa aaacacatgg attaaagttg ctgccctaac
     6361 cgtaacttgt attatcacat ggaatgtatt tattcatttc caagaaaaga agatgaattc
     6421 aaatatggct cattttttca aattagataa atatgaaaaa gcaattaatg acccaagatt
     6481 aatagatgac ctcgaaaaga acaaagaaaa agaaactata gctaaagcag aactagcgaa
     6541 agttgctcct tatattcatt tatacaatga aaaaggtgta gattttgtag caaatgaaat
     6601 agtaaacaat attgatttag atagagttac aaaattgcat attgaagata atctgaaaat
     6661 atttttcaaa gtagaacaat taaaaattct agctcaaaaa gatagtacag aaaaacaatc
     6721 ttatcaatca gcagtagatg ctgttgatgc tttaaagcca catattggct tatacaaaga
     6781 attgagccca gatgcttatg caaaagctat gatggctaaa gtagccatac aacctaataa
     6841 aatcaaggaa tttaagcaag ttttaaacta cgatattaaa agtgatattt caaatgaaat
     6901 ttctagagct gataatagta ttattcgatt tggattaatt ggcatgctga taattataat
     6961 gctttcattt atttataatg aaaaaattaa ggaacctagt gcacaaagaa ccgctaataa
     7021 ttttaaaaac ttacaattat tatcgtcagc gactttcagt attggacatg gcggaaatga
     7081 tgcacaaaaa gtaatgggta ttattgctgc ggctctagtt gcaaatggaa gtattactga
     7141 tattaaaaat atgccagatt gggtaccact ttcttgttat ttagcaatta gtattggtac
     7201 tttgagtggt ggctggaaaa tcattaaaac catgggtagt aagattacaa aagtaacacc
     7261 gttggaaggt gtttgtgctg aaactgctgg tgcaacaaca ttattcttaa ctgaacaaat
     7321 gggaataccc gtttcaacaa ctcatactat aactggtgca attattggtg taggagcaac
     7381 aaaaagacta agtgctgtgc gttggggtgt taccattaac ctactttggg cttggatatt
     7441 aaccattcct atttctgcat tgattgctgc tattacttat ggaattgtaa atatatttat
     7501 gtagaaaaat acgtgtaaga caaaaacttg agaatgtatg tatttatcta taacacattc
     7561 acttcaggag aaagtacaat attaaactta acttttacag attgaattac attttgggca
     7621 tgttcccaaa tttcagttcc agttgcttcg ccataattaa ccaccaccaa actttgtttg
     7681 gcatgattac cagtatttcc aataactttt cctttaaacc cacattgctc tatcaaccaa
     7741 gcagcaggga ttttcactga attgtcatta ataatataat aaggaatttc aggatacttg
     7801 gtttgtaaac ttttgaataa tgatggatta atttctgggt ttttaaaaaa actaccagca
     7861 ttacccaatt cttttggatt gggtaatttt gaagttctaa tcgctattac cgcattggct
     7921 atactttcaa cactaactgg tttatcaccc aaataatctt ggatatttcc ataagaagta
     7981 ttaaaattaa aatctttttt tttgagtttg atagtaatat tacttatgaa atacttattt
     8041 ttccattctt gtttaaactt gctatctcta tagccaaaag cacagtcaga tgctgatatt
     8101 gtcttctcct ctcctgttga taaatctgta acaaaaatat tttccaaaac atctttaact
     8161 tctacgccat aagctccaat attttgtatt ggtgccgcac caaccgttcc tggaatcaat
     8221 gctaaatttt caattccttg ataattattt tgaacacaaa acaatactaa atcatgccaa
     8281 attgttcctg attgagctgt tatccaaact tcactatctg tttcttggac aatattgata
     8341 ccctcaattt catttttaat gacaagtcct tcaaaatttt tagtaaataa aatattactt
     8401 ccaccaccta ataatagaaa tggctgttcc ttcagcaatc cattaacaat taataattgg
     8461 atatcttctt tcgttttaac aatgcaaaat gaatgagcaa atacttctaa tccaaaagta
     8521 ttgtagggtt ttaatgaaaa attagtgagt gtttccatca tccaattaag atattatgca
     8581 attttgcttt ttgagcctgc acaacttgtt caaactgcgg catttgattt ttaggaatag
     8641 atttacttac attcaacttt tcttttaatg gatcgacttg ttttccattt ttccaaaaac
     8701 gataacaaac atgaggtcca gttgccaaac cagtgctgcc aacatatcca attacctgac
     8761 cttgcttaac acgagtacct tttttcatgc ctttctcaat tttactcatg tgcaaatatt
     8821 gagtttcata tactttatcg tgtttgattt taacaaagtt tccattatta cttgtagaac
     8881 cagctacttc aacaattcca tcagccgttg caagaattgg tgtgcctttt ggagccgcaa
     8941 aatcagttcc caaatgtgcc tttacttcca tcaaaacagg atgaaatcta ctcaaattaa
     9001 atcttgaggt taaacgacca ttctttaatg gagattttaa aaaagtcatg tgtgtatatg
     9061 tacctttttc atctaaatac tgctgcgttt ttgtcccttc gggttgataa tttgttataa
     9121 aataattttt ctttttagta attacttctg ccgattgaat cgctcctgtt tcataataag
     9181 taccattaat aaaatactct gaaaataaaa cacgtaatgt atctccagtt ctaagttgtc
     9241 ctacagaaat tttattattg aatgcttgat tgattttatc aatcaaagca acattaatat
     9301 ttttagcttt taatatttta tggactttat tatttacgac aaaagccaat tgttgttgaa
     9361 cagtatcaat aggtttagca taaatactaa ttgctgtttt attattacaa tctataatta
     9421 aataatcttt ttggtccaat tcaaatgcaa tgaaatcgac agtagaaaca gagtctttaa
     9481 ttgaactaaa tgtaaaatat ttttgttttg cttttatgga ttcaaattca tcaataccat
     9541 ctaattgttt ggttaatgtt ttgatttctt ttttagaata attcaatctt ccaattattt
     9601 tacttaaaga ttctcttctg ccaacttgat gttcttctac ataaaattta gcaatatcaa
     9661 tacctaagaa ttctgtctta attgaatcga tacacttttt ttcttgcatt tttacaactg
     9721 gcttaacaaa ctttttgtgt ttgttatcaa taggtcttcc ttgtgcaaat ccagatgcaa
     9781 tcatataaaa tgacatcaaa ataatcacaa atgaacctgt aaaaatcgtt aattctcttt
     9841 tcatatttat ttattgctta gggacaaata tgtatatttc ttcactatct tttttcatcc
     9901 aatatttctc acgtgcaaat ttctcgatag aatttctgtc cttaaaaata tctgcttgtt
     9961 gctctttaat ctcaacaata gatttctcat agaacttttc ttcttttgaa agctgacgca
    10021 attctttgaa caaactgaat tgagttaata gattatattg attaaagaaa gttaaataga
    10081 caagataagc cacaacaata taaaagactt tgcttttaaa aatgcttggt attttctctt
    10141 ttaattctgg gtcaattagt ttcatactat attcagctac acaaaaatag gtacaaagtc
    10201 ttttaaaaaa aagttaaaag cctagatttg caatttttaa gagtttttta taaacttctg
    10261 acaaaacata agtatctgtt ttacaataac ttctaagttt atcataatat aattcttgct
    10321 cttctttgga tttataaaat agttcattat aaattgccat ggcttcttgg ccatctttta
    10381 catcaactgc attatatgga ttaatatcta acatagttgc tgaaatagtt ttcaaagaca
    10441 gactacccaa ttgttttgga tggtaataat ctaggcgttt aaatggaatt tcgacatcta
    10501 acataatcgc aatcctttta tcaatttcag gtttgaattc agggaaaaga ttagccaaat
    10561 aatacaacac acctttttca atcaaatcat tatatactaa aatcgtttta caggtttttg
    10621 tatcttctaa aaactgtttg ataaatgtct ttctataatc tccatctgtt gccgaaaaat
    10681 aatctttatg aataagcgtt gcatttactt catcttggat atgcaatgaa tataaaaaag
    10741 ggattcgctc ataaggcttt gtaccatcaa agattggaat agcactctga aatgcttcta
    10801 catcaaaaaa acaaattgga tattgtacat ttttgaaata attagcaata ttttcttttt
    10861 gtataatttc tttattcgtg aggtaactat gtaatttgat atcatcttga tgtttttgag
    10921 catattcatc aattgttaaa ataccagtat tgacagcttc caatttatca ttcaaggaaa
    10981 caccttgcaa aaaccaaatt gagttcgctg gaatatcttt ccaacaataa ctcataaaat
    11041 cacaaggata aggttttgaa caatgtgcac caattgcaat ttcaggcatt tgtgttttac
    11101 ttaaaacctg aattgctgca tctattttct cttgaatata aggttgtaat tctactattt
    11161 cttttagtac agattttctt ttaaataatc tatcaacttc taatgcacca ttctttacat
    11221 aattattatt aatatgaaca atttgaaaat cggcaaaatg aataccaaaa tgttgtatca
    11281 cataatattg aatggcacaa tcttgaatat aagtttgaga aacactcata gaacttttca
    11341 cttcataagc ataaaaattt ccgtctttac attctaagat atccaaagcc accaaaatac
    11401 cttgaaattt gaaagcagcc tcataaataa ttggataatt attttctatc aataattttg
    11461 ttgcagctat gctttgttgg taatgcatcg gacttggtgg agtacaatct ttccctccag
    11521 gaaataattg ttgagctaat tttccgactc gatgtcctct atcaaacttt tgttgttgtt
    11581 tcctgtctgg aatatctctt aaattataat gatttttata taggtataaa gatttaagac
    11641 attggataga acgaataagt gtggatttac taagttgatg acttttagac gacattaaaa
    11701 gttttttatt ataaataatt tagtattttt gaactttgat tgcacaaaat atgaaaaaaa
    11761 atacgttttt agttttaatt ctattaacag gattaagcac ttttgcacaa aaaggctttc
    11821 aagttggcgg aaatttcacc actggtttgt cttatatgtt agctcaaaat aactactttt
    11881 tagctccaaa tgttcaaatg aatggtgcta attctagtaa agaattggat tacaaagcaa
    11941 aatttagcta taatactggt ttgttttttg ggtataactt tgcggaaaaa catggtattc
    12001 gtgtactagc tggttatagt attgaaggac aaaaatatca agatattttc aaatggccga
    12061 tttatggttt acaaggacaa catactaaag aagttaattt tcagtttgta aacactaatg
    12121 tttcctaccg ttttgctcca attttaaaag gtcaaaagga aaaaaatcca aatggcaact
    12181 atccaaatgg tcagtataaa attagaatgc gtttgctagt tggcttagaa gttgatgcat
    12241 tagttaatgc taaaatgaaa tataagattg agcgattaac agaagaacaa ttaagagatg
    12301 aaaattggca aggttggtat gacccaaatc ctgcacccga tttttctgaa tatccataca
    12361 ttaaaccagg aaatcctttt ggcttaccac ctgcatatcc agcagttcag ccttatggaa
    12421 atggaggcta tgctccatat tatgcactag gaaagcctaa atctcaaaaa gactattttg
    12481 ttcctttaca agctggctta actataaact atggcttcga ctatatcttt aaaaataata
    12541 tgtacttcgg tgttggacta gatgcaaaag ttggcttaaa tgacatcaat tctaaacctt
    12601 atagaacaca cccagaatac aaaaaatcta aaaattattt tgtgggtcta aaagttgaaa
    12661 ttggctataa tattttaaaa gatgctgata aaacacctaa gaaaaagacc tcttcgtctt
    12721 caaaaataaa aactgaaaag gctccaaaag agaaagcaga agaccaagtt aaaacacctg
    12781 aatctgttag tcaatctaag gaaattggcg aaaaagctta cactaaatac aataagtacg
    12841 gtcagaaaaa agatgtaaaa atcaagagat tgaaaaagaa aaaataattg acttatttat
    12901 ttcataaaat aaagccattt ctgaagaaat ggctttattt tttttaatta tcttgcttaa
    12961 accattctgc aaaagatgtt ggagtttctt ccaatcgaat actgatgagt tgtatatttt
    13021 caggaaatag agattggatt gaatgtacaa aatgtaataa taaattttca caagtaggtt
    13081 gaaaaggtac taaaattact ttttcaaact cattcgtaat tacatcagat tttgaataag
    13141 gtgcttcttc attcaaaacc aaagcatggt cataaatgtc aataatttgt ttttttacaa
    13201 taccttttag ctctccaaaa tcaatcacca tacccaattt tacatcagca ttattttgaa
    13261 ttggtgttcc aattaaggtt acactcaaat ggtatgtatg accatgaata tttttacaag
    13321 gtccatcata accatataaa gcatgagcca tatcaaaagt aaattttttg gttactctta
    13381 ctttttgtgc attcataaat caaaatcttt ggaacaaaat tacaacttga ttgtgaaact
    13441 gctgtattta taacgttaat tcatagtctt atctaataag cttataaatt ctttataaat
    13501 attaagtgta attctattat ccaatttctt atctcctatt tgaccaactg tttgaattcc
    13561 tcgaacagca ttggtcaaaa atatttcatc agcttgctca atttcttctt tatacaaagc
    13621 cttttcaatg atagtaaaac tacgaatatg ctttaataaa aactgtctca aaacaccatc
    13681 aataggagca tcttttattc tcgaagtata aatctgattt tctttgacaa taaatacatt
    13741 ataaattatt gcatctgata aaactttttc tgaattaaat accagcacat catcaaaaga
    13801 gttttcaagt gcaaatttct tagctttgtc gtaaattaat ctatcggtgg attttaaatt
    13861 tgagtgtata ttttgttctt tataagtttc atcataaaaa ccaattttca aaggtatttt
    13921 atttatttca aacgttgaag tatacaactt ttgtgaactt accaaccatt ctacaattct
    13981 agtttcatta attttgactt gtattcttaa tcgaatattt tgactatcct gaattttaca
    14041 ttcttgttga atatagttag atatttttct aaatatagtt tcagaatcaa tattaatttt
    14101 taatagctgc aaactttgaa tcagtcgatt tgtatggtcg tttaagaaca ttgcatatcc
    14161 atttgaaaca cgaattgtct ctattaaacc aaaatcagtc ataggataaa aatacaaaat
    14221 atctttatcc tacaactttg ttatgttcaa taaaattgta tttattttct tagcacagct
    14281 aatgcatcac tccatctttt aagttcattc aacataacat ttgctgattt ttgaaccatt
    14341 tcatctgcat taaatttgcc ttcattatcc aagcttttga agaaaaaagg aattgctact
    14401 tgttcaacaa tcggcatcat attaaatgag gtaaccactt gtttaaacat ctgtgcagaa
    14461 cgcaatccag ctgagattcc accataacta acaattccaa caggtttata tttccattca
    14521 aaatgtaagt aatccattgc attttttata gttgcaggaa taccaaaatt atactctgct
    14581 aatacaaaaa taaatgcatc tgcctcatta atttttttac tccaagcttt tgtatgttct
    14641 aattcatact tttgtgccat tgggtggttc gcttcatcca taaatggcaa atttatttca
    14701 gccaaatcca acaattctac attatataac cctgattgtt gtgctaattc agtaatccat
    14761 tttgcaatat actttccatt tcctacggtt ctacttgtcg atgtaataat ttttaaattt
    14821 gtcatattgt ttaattttat ggtacaaaaa tannnnnnnn nnnnnnnnnn nnnnnnnnnn
    14881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14941 nnnnnnnnnn nngcattttt tcctacaata accaattcta tattttgata taaatacata
    15001 tccaaaacaa ttgcccaatt tgcatagtat tgaccatttt taactacact atctaaaaca
    15061 ttattaagca tagccacagc aatatgttca tattcatgat tatctaaata tttactcaaa
    15121 tacaataagc attttgccat ttgtgaattt gccgcaggaa tcacattatc gctactatcc
    15181 atacttctca cgaccaaagc agtatctaaa tttgatgtaa tataaaacat cttatgctct
    15241 gctgaataaa aatgttgaat acaatacgct gttaattgtt gggcaatttt taaatattgc
    15301 tcatcaaatg tcgattgaaa taataaaatt aaacactcaa taaaaaatac ataatcagcc
    15361 aaaaatccat taatgcttac tttatcattt ttaaaacttc tgtatagtct attatccgat
    15421 tgcagtactt tatctattat aaaattagct attgaaattg ctttattcaa aaataattga
    15481 tctccaaaag ctttataagc attcatcaaa ccaataattg ctaatgcatt ccattctgta
    15541 agaattttat catctaaatg aggtctaatt ttatgttctc ttgctttagc taatcgtgca
    15601 ttgatttcat ctactaattc atgaaaaact ttttgttcta attgatattt ttcacagaaa
    15661 tattcatgtt cttttgttct aaaaagaatg ttcattccat gctcaaaatt accttccttt
    15721 gtaatattgt acaattcaat tccaaagttt ttaatatcaa tatgcttgca attttgatga
    15781 tttatatgag ccattatttg aataaattca tcataattcc aacaataaaa tttaccttct
    15841 acaccttcgc tatctgcatc taaagcggca taaattccac cacttttatc cgtcatttct
    15901 tcatttatcc aatctaatat tttataacaa acacttttat acataggacg ttttgttatc
    15961 tgataagctt gagcataaac tgttagcaac tgagcattat cgtataacat tttttcaaaa
    16021 tgtggtactt tccaatatgc atctacgcta tacctcgcaa atcctccagc aacaatatca
    16081 tgtatgccac cgaaagccat tttctttaaa gtagttttca aataagcaga aagtttttca
    16141 tctttagttc ttgattgata cctaagtaaa aactctaatg caattggcat tgcaaattta
    16201 ggcataccca aagttccacc ttcttcatag tctaatttat ctttccaatt cttccaaatt
    16261 tgatgataaa atcgttcatc aatttctcta acatcggtat tgaaatgaac tgtatcgacc
    16321 aattgtatgc ctttttgaag acgttctgct tgtgcaagca ttttctcttt atctgtttca
    16381 aataaatttt gaaaatgatg taaaaattgt agccatcttt cttttggaaa ataggttgct
    16441 ccataaattg gtttgccatc aggcaaacaa acaacgttga gcggccaacc tccttggcca
    16501 ctcgtcattt gagccgcatt catataaatt tggtctatat caggacgctc ttccctatcc
    16561 acttttatag caacaaaata ttgattcata actttagcca cttcctcatc ttcaaaactt
    16621 tcatgttcca ttacatgaca ccaatgacaa gctgaatatc caacagaaac caatatcatt
    16681 ttttgttcct tctgtgcttt ttctaatgct acatcgttcc aagcatacca atttactgga
    16741 ttatttttat gttgtaataa ataaggtgaa gtttcgtatt gcagttgatt cattttgtgt
    16801 aaataattta gtttgtaata ataacaaatt aacgaaagaa aagatgttta aaataagaaa
    16861 ccaaacgttt gtttggaata tattttttat ttacttttaa ctgaaattga agacaaactt
    16921 catataaagt ataaatttat tttttgtaaa actaatagat tcgatagctt taatctgtgt
    16981 aattcaaaaa aatttaaaca agaaaatgat aaacaaaata accaaattat tagtagccaa
    17041 tcgaggagaa attgcaataa gaattttcaa aacttgtaaa gaaatgggca tttcaacagt
    17101 tgctgttttt tctgatgctg atgaaaatag tttatttgta aaatatgccg atgaagctgt
    17161 taggattgga ggcaaacaac ccaatgaatc ctacttatta atagataaaa ttatagatgc
    17221 tgcaaaacaa acaggagcca atgccattca tcctggttac ggctttttat ctgaaaaaga
    17281 aaattttgca caacgctgta tagatgaaaa tattatttgg gttggaccaa atcctaatgc
    17341 catagaaaaa atgggcagta aaattggtgc aaaaacaatt atgcaagcca acaacgtacc
    17401 cactattcca ggttatcaag gtgaagacca aagtgaagcc accataaaag caaaagcaat
    17461 agaaattgga tttccagttt tgttgaaagc aagtgctggc ggtggcggaa aaggtatgcg
    17521 tattgtacgt caagagaaag aattggacaa agctataaac gaagccaaat ctgaagcaat
    17581 gaatgctttt ggtgatagta ctttattaat agagaaatat tttgatactt ctcgacatgt
    17641 ggaatttcaa atttttggag ataaacacgg caatgcaatt catttatttg aacgagaatg
    17701 tagcattcaa agacggtatc aaaaagtggt agaggaaagt ccttcgccat ttattactga
    17761 agacacacga aaaaaaatgg gtgctgctgc aattttagct tgtcgagcaa ttcaatatga
    17821 taatgctgga actgttgaat ttattgtagc tccagaccaa tcattttatt ttttggaagt
    17881 caatacacga ttgcaagtgg agcatccaat tactgaagaa gttactggat tagatttagt
    17941 acgaatgcag attgaagttg cagaaggaca agctttgcaa gtcaaacaag aagccttaca
    18001 acaaattgga catgctatcg aagtacgatt atatgccgaa gatcctgcta ataattttct
    18061 tcctgtttct ggaaaaataa ttgattggat tccttccgaa actgatggtt tgcgatatga
    18121 tactggaatc gaaagtggct ctgaaatatc tactttttat gacccaatga ttgctaagat
    18181 aatagccaaa ggcaataatc gtcaagaagc gattaataaa ttaaatctag cacttaaaaa
    18241 tactgtcgta actggattta caactaataa aaattttctt tcagccattt tagaaaatca
    18301 agattttaaa gctggaaatt tcaataccca ttttttggat aaagttttta attatagtgg
    18361 agagaactac ccacaaacaa ccttagatat attaacttgt gctttacaac attatcgttt
    18421 tctgaaaaga aatgataaaa gaagcatagc caaaggtgtc cctgctggct ggcgaaataa
    18481 tttctatcaa gcacaaaatg aaaaatatgc ttatcaaggc tttgatttta tagtagaata
    18541 tgtgaataaa aatgagtact tagaaatttc ttttgcagac aaaactttta tatccaaatt
    18601 tataagcaac catgataaag tgtatatttt tgagatgaac aatattagaa gagcatttta
    18661 tttttcacac aacggcaatc aatatttcat taacgctccg caattaggac aaattcagtt
    18721 gaacatagtg gataggtttc cactaccaat agaagaagtc gtgaaaggag gatatatcgc
    18781 accaatgcca ggcgaagtaa caaatgtttt ggtcaaaact ggcgatattg taaaagctgg
    18841 agatgcctta ctgaaaatga tttctatgaa aatggaaaac accatagaag cacatactga
    18901 tggagagatt gaagaaatct atgttacaga caaacaattt gttgaatcag gaacactatt
    18961 gttgaaaatg aaggcataaa aattcttcaa ataaaaacaa tttataaaaa taagtttaat
    19021 gggttactat tgaaatccta ttaaatttaa gatgaatttg atgaaccaaa tcaacaccaa
    19081 ttccattttg aactaatttc aaattaacac gatttttttt aggatcactg agataccagc
    19141 atttttttga cccgctgaac tgtactgatg gaaagtgttg ctttagttca tttctcaaag
    19201 tagcatcatt tggaaatagt acccaaatta catttttgtc cctatgttgc cctatctcaa
    19261 atttatattt attcagttca aatcccagta tatattttac atattgttat aaaaaatacg
    19321 aaatacaata ttacgctaaa tattacgcta aatattacac aaatacaaat aacacgcaat
    19381 aagacatcta tatatattta attagcttac aatttcttat aaatatttta tatttttatt
    19441 tgataaatta aaattattta ttatatttgc gtatataaag gagttacctg ttatgttaga
    19501 aaaacgactg tgcaaaataa caacgacaca ttttgacgta atggcatttt aactttgcgg
    19561 aaatgaaata ataaattagt agtcaataat ttaataactt aaggaatttt ttatggaaaa
    19621 gaaaacatta tcgaacattt tggaagggaa aatctttcaa ataccagact accaaagagg
    19681 ttatgcttgg gaagaaaaac aatggaaaga ttttgtacaa gatattgatg cactgataga
    19741 tgataaaatt atcagccatt acacggggac tattgtaatt taccaaccaa gcaaaaagcc
    19801 aacggaaaat tacggaacaa aaagacttga aattgtagat gttgttgacg gacaacagag
    19861 actaacaact tgtagtcttt acttatcaat cataattaaa gaacttataa aaatcagtca
    19921 agaagatttt aatgctgaaa tccccatata tctttattct ggaagtaaga gcaagttaag
    19981 actaaacaac gacactgcgg acttttattt agatttaatt tccaaaggtg ttagtaatat
    20041 agaggcaaac tctgttcatc aaaaaagaat ttatgatgca tactgctttt tgaaaaaaca
    20101 tattgaagaa caattgacaa aaagagttga taaaggcgaa gattatttaa gagatttatt
    20161 tgatgctata attcgaaaac ttaatttttc tttttatcca attgaagtag aaagtgaaat
    20221 aggaatgacc tttgagttaa tgaattcaag aggaaaaaat ctatcctcta tggaattact
    20281 caaaaactat ctgatgtatt gggtttacag gaatatcctt gataattcag aaaaggaaga
    20341 ttttacgagc aaaattaata agacttggaa agaagtttat gtaaatattg ccaaatgtaa
    20401 tggaagtgaa agccaatgtt tacggatagc ttggacatta tttgtgaatt atacaccaaa
    20461 aaactggaat ggatacagtg gttttaaatc tgatgaagta attccattga gaaatttcaa
    20521 ctcaaagagt aaagaagatg taaaagagtt tttattaaaa tttgtggacg gacttgctct
    20581 aatttcaaaa cattattcag caatcatcaa acccaataac aattcttttg acgaaaacga
    20641 gttaaatctg cttactaaaa ttagaaatgc tggtaatatt gctaattacc ttcccttaat
    20701 ggttgcatca agaattaaaa aagaaacaaa cgaagtaact aaagatgatt attttgattt
    20761 gttgaaatca cttgaaatct tttcgtacag agtgtttctg tgggaaggaa aaagaagtaa
    20821 tgcaggttta tcaaaattct atcgttgggc tgatgatgta tttagttcaa aacactcatt
    20881 gaaatctgtt accgattgga tttatggaac aataaactgg tattcacctg aaaatggctt
    20941 taggaaatcc cttactgaag acttttttgg ttggtatcat tacagaagac tactgaaata
    21001 cactttattt gagtatgaac ttttcctctt aaaaggaaaa aataaaccaa aattaaactg
    21061 ggaagattta actgactcaa caattgaaca tattttgcct caaaatccag atagtaattc
    21121 taattggctg attaaatgga ctgatgaaga gcgaaaaaaa tatttgcacg acatttccaa
    21181 tttagttctt accaaagata attcacgata tagtaacttt gaatttgata gaaaaaaggg
    21241 tatagctggt tctggttttt catattcaaa ttcagatata agacaagaaa gaaaaattgc
    21301 ggaatatgac gattggactc ctgatacttg taaaaagaga agagaggaat taacaaattg
    21361 ggtcattaaa aactggggta ttgataaaca ttaccaagaa ccaatagtag atgtaattga
    21421 agaagatgat gagcctattt ttaatgatgt agattaaaac acagcaggta acagcgtgta
    21481 tgtggcaata gcgggtgaag tggtgaatcg aaggtcagtg cttctaagaa actttgtggc
    21541 aaacggaaag gaaagtgctt ttaatccgct actgcacata cacgcaaacc gttagcggta
    21601 attttaaaag actgacatag agaaaaaata taatgacaac acctgacgat aaaattatta
    21661 aaaccagaca gaaaatgtat caaatggaca gtggcaactt tgatgacatt tgctttactg
    21721 tatttcgctg tccatcatgc gggagggaga caatggactt aactctttta ccaatagttg
    21781 acaaggaaga ttattcagat atagac
//
LOCUS       DAOQCW010000030        21371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_897_length_21371_cov_6.109871, whole genome shotgun sequence.
ACCESSION   DAOQCW010000030 DAOQCW010000000
VERSION     DAOQCW010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 21371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21371
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_897_length_21371_cov_6.109871"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..1248)
                     /locus_tag="PLS16_04175"
     CDS             complement(148..1248)
                     /locus_tag="PLS16_04175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ60311.1"
                     /translation="MKSEKSKRIIFLVSNDLTTDNRMHRICNSLATSSFSVELVGRML
                     ENSKPLSKNRFLPTRLKLFFTKNFLFYAELNIRLFFYLLMNRFDIVCACDTDTLPAAF
                     FANLIKRKKIVYDAHEYYSETPELVGRNFTQSFWQVIEDYIIPKVDFAYTVNQSLANI
                     FEKKWHKKFEVIRNLPIKSSSLEPKNSSPYILYQGAINMGRGIKEMLLVMQFIENVPF
                     YIVGDGDESDKIKQLIQHYKLENKVKLLGRKSPEELKAITHQAYIGVNLLEQRGLNYY
                     YSLSNKFFDYIQAGIPQVCIGFPEYIAYNEKYKVAVLVERLVIEDIVNAIKKILENKD
                     LYNELSVNCIQAAKELNWELEEQKLIELYKQL"
     gene            complement(1229..3127)
                     /locus_tag="PLS16_04180"
     CDS             complement(1229..3127)
                     /locus_tag="PLS16_04180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HNJ60312.1"
                     /translation="MPEPKEILKKYWNYDSFREPQLSIIESVLEKNDTLALMPTGGGK
                     SICFQVPALVQDGICIVITPLIALMKDQIAQLKQRNIKAAAIFSGLSYKEIDVILDNA
                     VFGYYKFLYVSPERLKTELFIERFKKMPVNLIAVDEAHCISQWGYDFRPPYLEIAEIR
                     KHHPEVSILALTASATPEVQKDIIEKLKFKSENIFKKSFIRENISFVVRHEIAKLNKI
                     VEIAQKLGGSGIVYVSNRNKTKEIAEYLFRNNISADFYHAGLTNDERSIKQENWIQNK
                     TRIIVCTNAFGMGIDKPDVRFVIHIDIPETLEAYYQEAGRVGRDGKKSYAVALYTESD
                     KQYLETKVKEKFPEIEQVKKIYNSIFNFYEIAVGSGEFQTKTFDIQRFSKTYNYKPNE
                     VYYALKFLEQEGYIQLNESFFIPSRLMFAMDNLELYKFQLANTPYEPLIKSILRMYGG
                     IMSNFTRINEYDLAKGLRINVNELKKQLYYLRKRKVLYYEEKTDEPEIFFLVERLHQD
                     NLYFNWKYINERKRITFYKLEAIQKYIESESKCRQEIICNYFGETVMSCGKCDVCIEQ
                     KQTISITKKIEFFRSEILEKITNQFTTIDYFKPTNKLQKTAFELAVRSLLDDELIELN
                     YKNEIRKK"
     gene            3199..3696
                     /locus_tag="PLS16_04185"
     CDS             3199..3696
                     /locus_tag="PLS16_04185"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNJ60313.1"
                     /translation="MFKKEFFIFILVVCVAALLYWWYFKPPSNPMEKVKFQTEDGKTK
                     ELSDFRGKVVVLSYFQSWCSDCRRELPELLALQKTVGGEDVLTIIMVSDENWDKINIV
                     KNIANSPNLEFLQSTETLKSLGIKRYPTTYLLDKSGKEIKVDVEGIYWNNPEIIQIIN
                     KINQK"
     gene            3693..4169
                     /locus_tag="PLS16_04190"
     CDS             3693..4169
                     /locus_tag="PLS16_04190"
                     /inference="COORDINATES: protein motif:HMM:NF036524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60314.1"
                     /translation="MSLRESFEHALSVCAIDEAYIAYSAREKYLAFAYTYIQTIQKDT
                     KKLEPLLASKKIFIVPNDEVRRLKEVFVPYAKELIEEGVRNGEVVMRPILHQGYALLI
                     WQNFIQVLHFWANDKSIAKEETDVVIEKSVHFVFDALAPNAIDSGLDYLKFLVQKR"
     gene            4278..5705
                     /locus_tag="PLS16_04195"
     CDS             4278..5705
                     /locus_tag="PLS16_04195"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNJ60315.1"
                     /translation="MLQTRLLLFFLIIFSLISKGQTVLDWNDCLNLMEKNNLQLKMNE
                     INIQLAAIDIQKNKLNYSPNVNAQSNYSLRIGKNYNYFENKYENQIVHYNDFNLNLQQ
                     PIFDGLLTPNQVQKSKFDYEALKLDNDALKRNLQMQTMTAYLNILNAKEQLEQAKIQQ
                     LSTIEQQTKTDAMIDAGALPESNRYDIEAQMATEEANIVMAKNQLNLALLNLKVLLQI
                     DLNEVIDVKVPDGIVNNNIAFDTVPNAVDVFSVAVTQRPEVLAQKFRIESAKKNIKIA
                     KSSNYPTLNFIGNINTFYTNQNKTAQQTITGNFIPIGFVEATNQIVLSPETSVKQVKT
                     PYFNQLGNLLNYSFGLGLTIPIYNKNTARFSVKQAEQNIKLSELNQQQTIFDLNNTIQ
                     QAYIKTITAKENYIAAKKMYESTLKSYETAAERVSAGLSTQLELNLAKNTLNNSLSRL
                     TQTKFEYIFNRKVLDYYQGKTIQID"
     gene            5713..7017
                     /locus_tag="PLS16_04200"
     CDS             5713..7017
                     /locus_tag="PLS16_04200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNJ60316.1"
                     /translation="MKNKYLYILGVIAVVAIIVALLVSKNKAKNASIEVELQKISTQN
                     IIQTVAASGKVYPVEEVKISSDVSGEIIELNVKEGEIVQKGQLLARIQAESYQAYVEQ
                     SEANLNNAKANAANLQSRLKQAEAQMLNAEASYNRTKGLFDKKILSKADLEQAETAYK
                     SAKAEYNAGKDAINAANFTTKSVAASIKDAKNNLNKTAIYAPMSGIVSLLNVKKGEKV
                     VGTLQMTGTEMMRIADFGQMEIRVDVSESEIIKIKIGDTANVQIDAYLDRNFVGIVSE
                     VANTSKGASGGALSTDQSTNFVVKVVIDRNSYADLLDENAKPFLPGMSATVEIKTKQK
                     NNVVAVPIQSVIAKDTIINGVSSTREYVFVNKNGIAQRQLVKSGIQNDTYIEITEGLK
                     SGETIVTGPYNTITKKLNDGDKISLIKEGEKKKPSTKEDEEE"
     gene            7030..7893
                     /locus_tag="PLS16_04205"
     CDS             7030..7893
                     /locus_tag="PLS16_04205"
                     /inference="COORDINATES: protein motif:HMM:NF015226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HNJ60317.1"
                     /translation="MDKYLELNKLENESDIFKGISQIFLQDNVVCGLVFTLAVCFNNW
                     IMGIALLVATIFGTLTAVLLKLDKNEINQGLYGFNAALVGVAMVLFFKPTIIVWILIV
                     IGAVLSTLCYAYFLKKKIPIFTLPFIIITWIIYFSFKPFLSPATQQIAQEQTSLDFVS
                     ILSKSFGQVLFQNNIWVGIVFFLALAWSSKVAALFGLIGAILSAILAYVFDFPIQDIN
                     DGLLGYNAVLCAIVFADWNYKALTLAIFSIMLSFVCLVLLKYFNFLPLTFPFVLASIL
                     TLKIKNKFVVL"
     gene            complement(8154..9653)
                     /gene="cysS"
                     /locus_tag="PLS16_04210"
     CDS             complement(8154..9653)
                     /gene="cysS"
                     /locus_tag="PLS16_04210"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNJ60318.1"
                     /translation="MSALHIYNSLSRQKELFTPINPPFVGMYICGPTVYNYVHLGNVR
                     TFTAFDVVYRYLKYLGYNVRYVRNITDAGHLTNEQGEGKNRMEDQAKLERIEPMEIVQ
                     KYTLSFHEVCSLFNLLPPTIEPTATGHIVEQIDMTEQLIQKGLAYEANGSVYFDVKAY
                     NNRGNNYGKLSGRNIDELLAGYRDLDGQDEKKSSVDFALWKKASPEHIMQWKSPWGSG
                     FPGWHIECSVMSTKYLGKRFDIHGGGMDLKFPHHECEIAQNIGACNEEPVNYWMHSNM
                     LNFNGQKMSKSLGNSILPLELVTGNHPLLDKAYSPMVVRLLFLQSHYASELDINIKSL
                     QDAEKNLRKLLNAANFLQEITQSGAIKPDIDIENQIHQLCLGCKEAMDDDFNTAKTVA
                     NLLGLVTHINTYYNNGKTANNISVDVYNSLQKYYIEYLFDILGLKNETQNADGTNETL
                     DKVMNLVIDIRKQARENKDWTTSDKIRDALKDAKINIKDNKDGTTYELI"
     gene            complement(9765..10166)
                     /locus_tag="PLS16_04215"
     CDS             complement(9765..10166)
                     /locus_tag="PLS16_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HNJ60319.1"
                     /translation="MENEKNFEQNSVYSKRVRAGKRRTYFFDVRTTKSNDYFITITES
                     KKKFEGDGYERHKVFIYKEDFMKFINSMQETVDYVKKELLPDFDFDAIYDYDQEHNHS
                     NDIPATPDAETISTSSNTTTSSANEEVDKWD"
     gene            complement(10245..12035)
                     /locus_tag="PLS16_04220"
     CDS             complement(10245..12035)
                     /locus_tag="PLS16_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ60320.1"
                     /translation="MKELRAVNFYFLKYKWLFLSGIFFVITSNYFGLLPPQYIQEGID
                     ILKDNIQLYQLIGHSETQAIFRNYLFGQFILFIAILVIIASLIKGVLMFFMRQTLIVM
                     SRKIEYDQKNQIFKQYQTLNTSFYKRNNTGDLMSRISEDVSRVRMYFGPAIMYSINII
                     ALFVMVIYSMVATNAYLSIFVLLPLPILAFLIYKVSSVINTKSEEISTALSNITSLAQ
                     EIFSGIRVVKAFSIDKLTQEEFAASSEVYKQKNINLAKIEAYFAPLMLLLVGLSTLMV
                     IYIGGIEVQKGNFTVGNIAEFIYYVNMLTWPVASLGWAVSLVQRAAASQKRINQFLDD
                     KNIIQSAQSPIIKDIIKIELKNVSFTYPDTGIDALKNINLQIKKGEKIAIVGRTASGK
                     STLAELLCRTYDVNKGELLINDENIKNINLNKYRALLSYTPQDVFLFSDTIENNILFG
                     NLEADKNKVEHYSKIACVHDDIMHFPKQYSTIIGERGVMLSGGQKQRISIARCLIKDP
                     ELIILDDCLSAVDANTERQILDNLEDAFEQKTVVFITHRIFAVMSFDKIIVLDDGQIV
                     EQGTHQTLMDLKGDYYDLYQTQLQENEVKN"
     gene            complement(12157..13284)
                     /locus_tag="PLS16_04225"
     CDS             complement(12157..13284)
                     /locus_tag="PLS16_04225"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNJ60321.1"
                     /translation="MILVCTTLLILSFNTKAQEAVDSTKTSVGEKIDKWQSKLPLNFS
                     GSVDAYVQTNFIPKQSNDVLNEAFSPKANSFQLGMINFMLSKEIKKVGFMADIGFGPR
                     AEAANNTLYANSIIAIKQLYMTYAPAEWVKLTLGNFSTFFGYEYIEPTANFHYSLSLA
                     FQNGPYYHTGFKANFTKNKFNFLVGFFNDTDHKDDKDRNKFVGSQVGYTGDKFSAYLN
                     YVGGNESNLHDTTDAFKNYKSSLGFTGSATLGKKSKGSMAIDFAWHRFRQIASKGDKA
                     THAQYFTTYLYSKYNIKDNVGIGARIGYSNNNDLAIPYVAGPAKHFIDVTLTSNITIS
                     DFLSVKPEFRIDYADKKVFLSRTGAGSNLQYRLMLATIFSF"
     gene            13984..14868
                     /locus_tag="PLS16_04230"
     CDS             13984..14868
                     /locus_tag="PLS16_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60322.1"
                     /translation="MNRIKEYLLIIVCLLGFQMYLKANMASPDLELLSGVSTISSQQI
                     DILHEDLKITLDPFFDKAFFDVHYRIRADKSGKQIPLLFVAEFYKSNFTVEVDGKPVA
                     ILQIPETFYESGRLKEYSPPNIEVKEDRVYVNWPVFQKDYEKKDVQYFEVDLTKGEHS
                     IVIRYESSSLTDQENNIVTKKSFKYLLSPAKLWKSFGGLSLTIDGTDFLKENPSTSIS
                     FDFGDLTPSEPMKRWEFDSIPVDYFYINYYPKVPKTIQPYIDYYKPAIIIFGGGMILL
                     LIIAYVVYRYIKKQKTQG"
     gene            complement(14984..15991)
                     /gene="recA"
                     /locus_tag="PLS16_04235"
     CDS             complement(14984..15991)
                     /gene="recA"
                     /locus_tag="PLS16_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNJ60323.1"
                     /translation="MSEKVKDENKLKALQLTLDKLDKQYGKGTVMKLGDNSIVESDVI
                     PTGSLGLDLALGIGGFPRGRIIEIYGPESSGKTTIAIHAIAETQKRGGICAIIDAEHA
                     FDKKYAEALGVDTDNLYITQPDDGEQALDIAEQLIRSAAIDLVVIDSVAALVPRSEIE
                     GDMGDSKMGLHARLMSQAMRKLTGAINKTSCSCIFINQLRDKIGVMFGSPETTTGGNA
                     LKFYASIRLDIRRTGQIKDKDGNVIGNQTKIKVVKNKLAPPFRTAEFDIFFGKGVSKD
                     GEIIDLGADLGVLQKSGSWYSYEGQKVGQGRDQVKQILADNPAMADEIETKIKEKLAT
                     I"
     gene            16202..17623
                     /locus_tag="PLS16_04240"
     CDS             16202..17623
                     /locus_tag="PLS16_04240"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HNJ60324.1"
                     /translation="MRKRQTSIFILAHSIGDIVILNLSIILGHIWSFWGKPIQIFSEM
                     YLQMWLYLNLIYFVSAQISGTFDMYRNTRFFTLVSSIFRLFFFQVVFAFSYIVIFKDF
                     NNTFKLSREALLVTYLSSFILTLIWRFGFIKMARFYRARGFNNRRIVIVGAGESAQDF
                     KKMLESRVEYGYKFIGFFEDRPERYNLKVRENILGSIDEVNAYALNNDIDEIFCALPY
                     SEEIKIKDLISFGDENLIRVKIVPDMTRFLSHHLNKIEIDYYGQIPVMTLRPEPLENF
                     INRVIKRIFDIGFSVLIITFVLSWLIPIISLIIKLTSKGPVFFKQKRSGFRNQTFDVI
                     KFRTMYVHDDSNAKQATKGDSRITPIGAFLRKTSLDEFPQFINVFFGQMSVIGPRPHM
                     LKHTDEYSKIIDKFMVRHLVKPGITGWAQVNGYRGETRNKQKMEDRVRADVWYIENWS
                     FTLDIKIVFMTIVNLIRGEENAF"
     gene            17613..19331
                     /locus_tag="PLS16_04245"
     CDS             17613..19331
                     /locus_tag="PLS16_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60325.1"
                     /translation="MHFRYILLAIFYFNAFGITIFAQQENLYLNREKSLTFEKKLNQM
                     DKASFHSAIKPYIASEVYDFVYPDSVIHYTKLQKSDWFSKMVNVVGFENFLEFDENGY
                     VKYRRDIVANPYLPTYNDVPSDTGYVNRKFYVAINPIINIGMGVDFYTSKIISQRTVG
                     FELKADIGKKVSLYTAITNNVVKFPSYITLYSVAATAVPGEGRTRPTSNNSFDYSSAI
                     AYLSYSPVKQFNMQIGNGKHFIGDGFRSLLLSDNSFNYPYLQFTTNVWRIKYTTIYTE
                     LLNNIKRGTDFTLGYQRKLATFNYLSVDAAKWLQLGVFEGITWRRTTDKGNTFFDYNF
                     INPILGVRAFQKKLNPNINKVYGTNIKVTLPKNIILYGQFAAQSWGKKNTAKRKTAFQ
                     VGMKYFEVGNVPNLNLQVEFNQVRPFMYQGLDSLLSYSNYNQSITHSMGANFREYLAT
                     LNYQWKRLYTSYKFNFTQAGYDFTGNFLNIENNGGNILKAEKDIVNSMNVKMFNGTPY
                     NIINNEFRIGYIINPKINFVAELGVQFRNYSSSSNDFTNFSSHSIGLSFKTNIFNRYY
                     DLPVLF"
     gene            19412..20155
                     /locus_tag="PLS16_04250"
     CDS             19412..20155
                     /locus_tag="PLS16_04250"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNJ60326.1"
                     /translation="MQKVFITLLLGLVFLSSFAQEQTVTATSGVSPEVKANINKAKDR
                     VLIELGLNQMLKTPSDLKVSALSRSFNAYYTYDVVFGKSNFSIAPGAGIGTDNFFHKK
                     NGIAWKNNTHLKDSITSFPLLGDSVSSKKSKLGLVYFDIPVEFRYRSKPNKKNTSWKL
                     AAGAKFGFLLASKWKYKGEDPATGTGGDVKFKEFKVDGLNKFRYGVYVRGGYGIFNVY
                     VNYQISSVFQKGKGPQMYPLQFGIAICGL"
     gene            20281..>21371
                     /locus_tag="PLS16_04255"
     CDS             20281..>21371
                     /locus_tag="PLS16_04255"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNJ60327.1"
                     /translation="MDKVYIFDTTLRDGEQVPGCQLNTVEKIEVAIALENLGVDVLEA
                     GFPISSPGDFNSVVEISKNVSNPTICALTRAVDKDIECAAEALKFAKKGRIHTGIGSS
                     DIHIINKLRTTRENILEKAAWAAKYARNFVDDVEFFCEDAGRADLAFLAKMVETVIAN
                     GATVVNIPDTTGYCTPDQYGAKIAYLMNNVPNIDKAIISVHCHNDLGLATANSIAGLS
                     NGARQVEVTMNGIGERAGNTSLEEVAMIIKSHKESLNLYTDINVKNIYPTSRLITRLM
                     RMPVQPNKAIVGRNAFAHSSGIHQDGVLKNRENYEIIDPKDVGVPESLILLTARSGRA
                     ALKHRLDLLGYHLDSEKLDNVYQEFLKVA"
BASE COUNT         7281 a         3063 c         3416 g         7611 t
ORIGIN      
        1 gctttcctat caataatagc ttttgttatt cttaaaatta aatagtttat taataaaata
       61 actgcgattg caataagcat tccaactttt agctcatact tggagaagct aaataaacta
      121 taatctaaaa cttcttctac attcatatta taattgcttg taaagttcaa ttagcttttg
      181 ctcttctaat tcccaattta attcttttgc tgcttgtatg caatttacac ttaattcatt
      241 atataaatct ttgttttcaa ggattttttt tatagcattt acaatgtctt caattactaa
      301 tcgttccact aaaacagcaa ctttatattt ttcgttatag gcaatatatt caggaaatcc
      361 aatgcaaact tgaggaatac ctgcttgaat ataatcaaaa aatttattgc ttaatgaata
      421 gtaataattt aaacctcttt gctctaagag attaacacca atatatgctt gatgtgtaat
      481 agctttaagt tcttcgggcg attttcttcc caatagtttt actttgtttt ctagtttata
      541 atgttgaatg agttgtttga ttttatcact ttcatcacca tcgcctacaa tataaaaagg
      601 aacgttttca ataaattgca ttaccagtaa catttctttg atacctcttc ccatattaat
      661 tgcaccttga tataaaatat aaggagatga atttttgggt tctaaagagc ttgattttat
      721 tggtagattt ctaattactt caaatttttt atgccatttt ttttcaaaga tatttgctaa
      781 tgattgattg acagtatatg caaaatctac ttttggaatt atataatctt caattacttg
      841 ccaaaatgat tgagtaaaat tacgaccgac tagctcaggt gtttcagagt agtattcgtg
      901 agcatcatac actatctttt ttcttttaat aagatttgcg aaaaatgcag ctggcaaagt
      961 atctgtatcg caagcacaaa caatatcaaa gcgattcatt agtaaataaa aaaacaaacg
     1021 aatgttcagt tctgcataga aaagaaagtt ttttgtaaaa aatagcttta accgtgtggg
     1081 taaaaatctg tttttactga gtggttttga attttctaac attcgcccaa ccaattctac
     1141 ggaaaagctt gaagttgcta atgaattaca gattcggtgc atacgattat ccgtagttaa
     1201 atcattgcta actagaaata ttattcgttt acttttttct gatttcattt ttataattca
     1261 attcaattaa ttcatcatct aataaacttc taactgctaa ttcaaaagcg gttttttgta
     1321 atttatttgt aggcttaaaa tagtcaattg ttgtgaattg atttgttatt ttctctaaaa
     1381 tttcactcct gaaaaattct atttttttag tgatagaaat agtttgtttt tgctcaatac
     1441 aaacatcgca ttttccacaa gacattactg tttcgccaaa ataattacag atgatttctt
     1501 gcctacattt gctttcggac tcaatgtatt tttgaatggc ttctaattta taaaatgtta
     1561 tccgttttct ttcattgata tatttccaat tgaaatacaa attatcttga tgcaaacgtt
     1621 ctactaaaaa aaatatttct ggctcatctg ttttttcttc ataataaagg acttttcttt
     1681 ttcgcaaata atacaattgt ttttttaatt catttacatt aatacgtaaa ccttttgcta
     1741 aatcatattc atttattctc gtaaaattgc tcatgattcc gccatacatc cgtaatattg
     1801 attttattaa aggttcgtac ggtgtatttg ctaactgaaa tttatataat tccaaattgt
     1861 ccatagcaaa catcaatctt gatgggataa aaaaactttc attgagttgg atatagcctt
     1921 cttgttctaa aaacttcaaa gcataataca cttcattcgg tttataatta tatgtttttg
     1981 aaaagcgttg aatgtcaaaa gttttagttt gaaattcgcc acttccgaca gcaatttcat
     2041 agaaattaaa aatagaattg taaatcttct ttacttgctc aatttcagga aatttctctt
     2101 ttacttttgt ttctaaatat tgcttatcac tttctgtgta taatgcaaca gcataagatt
     2161 ttttgccatc acgtcctaca cgtcctgctt cctgataata ggcttctaaa gtttcaggaa
     2221 tgtcaatatg tatcacaaaa cgaacatcag gtttatcaat tcccataccg aatgcattgg
     2281 tacaaacaat aattctagtt ttattctgta tccaattttc ttgttttatg cttcgctcat
     2341 cattagtcaa accagcatgg taaaaatcgg cactgatatt gtttcgaaat aaatattctg
     2401 ctatttcttt ggttttattt ctattggaaa cataaacaat gccactgccg ccaagttttt
     2461 gtgcaatttc tacaatttta tttagtttgg caatttcatg acgtacaaca aaactaatgt
     2521 tttctcttat aaaagatttt ttgaaaatat tttcagattt gaattttagt ttctcaataa
     2581 tatctttttg aacttcgggt gttgcacttg ccgtcaatgc caaaattgaa acttcaggat
     2641 gatgttttct gatttcggca atttccaaat aaggtggtct gaaatcatat ccccattgag
     2701 aaatacagtg tgcttcatca actgcaatta aatttacagg catttttttg aaacgctcaa
     2761 taaatagttc tgtttttaat ctttctggcg atacataaag gaatttataa taaccaaaaa
     2821 cggcattatc caaaataaca tcaatttctt tataacttaa tccagaaaaa attgcagcag
     2881 cttttatatt tcgttgtttg agttgggcaa tttggtcttt catcaaagca attaaaggag
     2941 taatgacaat acaaattcca tcttgaacta aagcaggaac ttgaaagcaa atagatttgc
     3001 ctccgccagt tggcatcaaa gccaatgtat catttttttc taatacactt tcaataattg
     3061 aaagctgcgg ctctcggaaa gaatcataat tccaatattt ctttaagatt tctttaggtt
     3121 ctggcatgct gtaaattgga aagcttatct tatgtttgta tcaaaaatag aggtaatttt
     3181 tgactatctt ccttttttat gtttaaaaaa gaatttttta tatttatcct agttgtatgt
     3241 gttgcggctt tgttgtattg gtggtatttt aagccaccaa gtaatccaat ggaaaaagtg
     3301 aaattccaaa ccgaagatgg aaaaactaaa gaactatctg attttagagg caaagttgta
     3361 gttttaagtt attttcagtc gtggtgtagc gattgtcgta gagaattgcc agaattatta
     3421 gctttgcaga aaactgttgg aggagaagat gttttgacga taattatggt ttctgatgaa
     3481 aattgggata aaattaatat cgttaaaaat atagctaaca gtccaaattt agaattcttg
     3541 caatctactg aaacgttaaa atctttaggt atcaaaagat atccaacgac ctatttgtta
     3601 gacaaatctg gaaaagaaat aaaagtagat gtcgaaggaa tttattggaa taatcctgaa
     3661 ataattcaaa tcataaataa aattaatcaa aaatgagttt aagagaaagt tttgaacacg
     3721 cattgtctgt ttgtgccatt gatgaagctt atattgctta tagtgcaaga gaaaaatatt
     3781 tagcatttgc ttatacttat attcaaacta ttcaaaaaga tacaaagaaa ttagagccat
     3841 tactagcatc caaaaaaatc tttattgtac cgaatgatga agttcgtaga ttgaaagaag
     3901 tttttgtacc ttatgctaaa gaattaattg aagagggtgt aagaaatggt gaggtggtca
     3961 tgcgtcctat tttgcatcaa ggttatgctt tattaatttg gcaaaatttt atacaagtgt
     4021 tgcatttttg ggcaaatgat aaaagtattg ccaaagaaga aaccgatgtg gtaatagaaa
     4081 aatctgtgca ttttgtattt gacgcattag ctccaaatgc tattgattct ggattagatt
     4141 atttgaagtt tttggtacaa aaacggtaag atttatattt gagaaactat catctgagat
     4201 tgattatctt ataaatatgt atggattttt tcaaaatata aaataataaa attacttttg
     4261 ttagataaaa aatagagatg ttacaaactc ggttgttatt attttttcta attatttttt
     4321 ctttgatatc gaaaggacaa accgttttag actggaatga ttgtctaaat ttgatggaga
     4381 agaataattt acaattgaaa atgaatgaaa tcaatattca gttggcagca attgatatac
     4441 aaaaaaataa actcaactat tctccaaatg tcaatgctca aagcaattat agccttagaa
     4501 ttggtaaaaa ctacaactat tttgagaata aatatgaaaa tcaaattgta cattataatg
     4561 actttaattt gaatctgcaa cagcctattt ttgatggatt attgacaccc aaccaagtac
     4621 aaaaatcaaa atttgattat gaggcattga agttggataa tgatgctttg aaaagaaatt
     4681 tacaaatgca aacaatgaca gcttatctaa atatcttgaa tgctaaagaa caattggaac
     4741 aggctaaaat tcaacaatta tctaccattg aacaacagac taaaactgat gctatgatag
     4801 acgcaggtgc tttgcctgaa agtaatcgtt atgatattga ggctcaaatg gcaacagaag
     4861 aagcaaatat tgtaatggca aaaaatcaat taaatttagc attgttgaac ttgaaagttt
     4921 tgttacaaat tgatttgaat gaagttattg atgtaaaagt tccagacggt atagtaaata
     4981 ataacattgc attcgatacc gttcctaatg cggttgatgt tttttcggtt gctgtaacac
     5041 aaagacctga agttttagct cagaagttta gaatagaaag tgccaaaaag aatattaaaa
     5101 ttgcaaaatc tagtaattat ccaactttga actttattgg caatatcaat acattttata
     5161 caaatcaaaa taaaacagct caacaaacaa ttactggaaa tttcattccg attggatttg
     5221 tagaagcaac aaatcagatt gtattgtcgc ccgaaacatc agtgaagcag gtcaaaacac
     5281 cttattttaa tcagcttggt aacttattaa attattcttt tggtttaggt ttaacgattc
     5341 caatttataa taaaaacaca gctcgttttt cagtaaagca agctgagcaa aatatcaaat
     5401 tgagcgagtt aaatcaacaa caaacaattt ttgatttaaa caatacgata caacaagctt
     5461 atatcaaaac tattactgca aaggaaaatt atattgcagc aaaaaaaatg tacgaaagta
     5521 cactgaaatc ttatgaaact gcagctgaaa gagttagtgc tggtttatcc acccagttag
     5581 aattaaattt ggctaaaaat accttgaata atagtttatc tagactaaca caaacaaaat
     5641 ttgagtatat ttttaacaga aaagttttgg attattatca aggaaaaacg atacaaatag
     5701 attaattgga atatgaagaa taaatattta tatatattag gagtaattgc tgtagttgca
     5761 ataattgtcg ctttgttggt ttctaaaaat aaagcaaaga atgcaagtat tgaagtagaa
     5821 ctacaaaaaa tcagtactca aaacattata caaactgttg cggcaagtgg aaaagtttat
     5881 cctgtggagg aagtaaaaat aagctcagat gtttctggag aaattattga attgaatgta
     5941 aaagaaggag aaattgttca gaagggtcaa ttgttggcac ggattcaagc agagagttat
     6001 caggcttatg tagagcaaag tgaagctaat ttgaataatg caaaagcgaa tgctgcaaac
     6061 ttacaatcaa gattaaaaca agctgaagca caaatgttga atgctgaagc aagttataat
     6121 agaaccaaag gattatttga taaaaaaata ttgtctaaag cagatttgga acaagcagaa
     6181 acggcttaca agtcagcaaa agcagaatat aatgctggaa aagatgctat caatgcagcc
     6241 aacttcacga caaaatctgt agcagcatca attaaagatg ctaaaaataa tttgaacaaa
     6301 actgctatct atgcaccaat gtctggaatt gtttctttgt tgaatgtaaa gaaaggagag
     6361 aaagtggtgg gtactttaca aatgacagga actgaaatga tgcgaattgc tgattttgga
     6421 cagatggaaa tccgagttga tgttagtgaa agtgaaatta ttaaaattaa aataggagat
     6481 actgcaaatg tgcaaattga tgcttattta gatagaaact ttgtgggtat tgtgagtgaa
     6541 gtcgccaata cttcaaaagg tgcgagtggt ggtgctttga gtaccgacca atctactaat
     6601 tttgttgtga aagtagtaat agatagaaat tcttatgcgg atttattaga cgaaaatgca
     6661 aagccatttt tacctggaat gtcggccacc gttgagatta aaacaaagca aaaaaataat
     6721 gttgttgcgg ttcctattca atctgtaatt gcaaaagata caattattaa tggcgtttct
     6781 tctactagag aatatgtatt tgtcaataaa aatgggatag ctcaacgtca gttagtaaaa
     6841 tcaggtattc aaaatgatac ttatattgaa attacagaag gtttaaaatc aggagagaca
     6901 atcgtaacgg gtccgtataa tacaattact aaaaaattga acgatggcga taaaatttca
     6961 ttaattaagg agggtgaaaa aaagaaacca tctacaaaag aagatgagga agaatagaaa
     7021 tttattgtag tggataagta tttagaatta aataaattag aaaatgaatc tgatattttt
     7081 aaaggaataa gtcagatatt tttacaagat aatgttgttt gtggtcttgt tttcacttta
     7141 gcagtttgtt ttaataattg gattatgggt attgccttat tggtggcaac tatctttgga
     7201 acattgactg ctgttttgtt aaaattagat aagaatgaaa ttaatcaagg tctgtacgga
     7261 tttaatgcag ctttggttgg tgtagcaatg gttttatttt ttaaaccaac tattattgtt
     7321 tggatattaa ttgtgattgg ggctgtttta tctactttat gttatgccta ttttttgaag
     7381 aaaaaaattc caatttttac attgccattt attattatta cttggataat atatttttca
     7441 tttaagcctt ttttgtcgcc agctacacag caaatagcac aagaacagac atcattggat
     7501 tttgtttcta tactatcaaa aagttttggt caagttctat ttcaaaataa tatttgggtg
     7561 gggattgtat tttttcttgc acttgcttgg agttcaaaag ttgccgcact atttggatta
     7621 ataggtgcaa ttctatctgc tattttagct tatgtttttg acttcccaat tcaagatatt
     7681 aatgatggct tgttaggata taatgctgtt ttatgtgcta ttgtttttgc tgactggaat
     7741 tataaagcac ttacgcttgc tattttttca ataatgctat cttttgtttg tcttgtacta
     7801 ttgaagtatt ttaattttct tccacttacc tttccatttg ttttagcatc tatattgaca
     7861 ctcaaaataa aaaacaaatt tgtagtgtta taaaaacaat gtgtatcaat ataaatacta
     7921 acccaaaaca atactactta atagatatag ttttttttta ttaaatacgt ttattcttta
     7981 ttattagatt attcccattt ttaaaaggag cagatgcgta ttttcaaacc caaacgtcag
     8041 aaacaatttt atttgaattg aatggcagaa atatttcagt ctataattgg aaatcaaaat
     8101 ctgaaggcgt aagcgtcgcc gtttttgaat aaaaattagg gattttttac tccttaaatt
     8161 agctcataag ttgtgccatc tttattgtct ttgatattta ttttggcatc tttcaaagca
     8221 tctcgaattt tgtcagaagt tgtccaatcc ttattttctc ttgcttgttt tctaatatca
     8281 attaccaagt tcatcacttt atctaatgtt tcgtttgtac catcggcatt ttgagtttca
     8341 ttcttcaagc ctaaaatatc aaatagatat tcaatatagt atttttgtaa agaattatag
     8401 acatctactg aaatattatt tgcagtttta ccattgttgt aataggtatt aatatgcgtt
     8461 accaaaccta ataaattggc cactgttttt gcagtattaa agtcatcatc catggcttcc
     8521 ttgcaaccta agcataattg atggatttga ttttctatat caatatcagg ttttattgca
     8581 ccactttgtg taatctcttg taagaaattt gcggcattta ataattttct taaatttttt
     8641 tctgcatctt gcaatgactt gatattaata tctagttctg aagcataatg ggattgcaaa
     8701 aacaataaac gcaccaccat tggcgaatag gctttatcta acaatgggtg attgcctgtt
     8761 actaattcta aaggcaaaat ggaattgcct aaagatttgc tcatcttttg accattaaaa
     8821 ttcaacatat tgctatgcat ccaatagtta acaggttctt cattgcaagc cccaatattt
     8881 tgtgcgattt cacattcgtg gtgcggaaat tttaaatcca taccacctcc atgaatgtca
     8941 aatcgtttgc ctaagtattt agtactcatt acagaacatt cgatatgcca acctggaaag
     9001 ccactacccc acggagattt ccattgcata atatgttcag gtgatgcttt tttccataat
     9061 gcaaaatcga ctgaactttt cttttcatct tgtccatcta aatctcggta gcctgccaat
     9121 aattcgtcaa tatttcgacc tgataatttt ccgtaattat tgcctctgtt attataagct
     9181 ttaacatcaa aatatacaga accattggct tcataagcta atcctttttg gattaattgc
     9241 tctgtcatat caatttgttc cacgatatgt ccagtcgcag ttggttcaat agttgggggc
     9301 aataaattaa ataggctgca aacttcgtga aaactcaaag tatatttttg aacgatttcc
     9361 ataggctcaa tacgctccag ttttgcttgg tcttccattc tgttttttcc ttcgccttgt
     9421 tcattggtta aatgtcctgc gtcagtaata tttctaacgt atctaacatt ataacctaag
     9481 tactttaaat atctatatac cacatcaaac gcagtgaaag ttctcacatt tcctaaatgt
     9541 acataattat atacggtcgg tccacaaata tacataccca caaaaggcgg attaataggt
     9601 gtgaatagtt ctttttgtcg tgaaagagaa ttgtaaatat gtaatgctga cataatgcaa
     9661 aaataattaa aaattgtata agaaactaga gcgtataaaa caaaacaacc taattctatc
     9721 cgaagatatc attaggttgt ccaattattt gtttaagtag taaattaatc ccatttatct
     9781 acttcttcat tagctgaaga tgtagtggta tttgagcttg tgcttatagt ttcagcatct
     9841 ggagttgcgg gaatatcatt agaatgattg tgctcttggt cgtaatcgta aattgcatca
     9901 aaatcgaaat caggaagcaa ttcttttttt acgtaatcta ctgtttcttg catagaatta
     9961 atgaatttca taaagtcttc tttgtaaatg aaaaccttgt gtctttcata accgtctcct
    10021 tcaaattttt tcttactttc tgtgatggta ataaaatagt cattactttt tgttgtacgc
    10081 acatcaaaaa aatacgttct gcgttttcct gctcttactc ttttagagta cacactgttt
    10141 tgttcaaaat ttttttcgtt ctccactttt ttctatttta attgttcaat aatactaaat
    10201 aaaaaattga tagaaaaaca ttttctcaag attttatcaa aaatctaatt tttaacttcg
    10261 ttttcttgta attgtgtttg atacaaatcg taataatcgc cttttaaatc cattaaagtt
    10321 tggtgtgttc cttgctccac aatttgacca tcatctaaaa caatgatttt gtcaaaactc
    10381 attactgcaa atattctatg agtaataaat acgacagttt tctgttcaaa tgcgtcttct
    10441 aaattgtcca aaatttgtct ttctgtattt gcatcaactg ccgacaagca atcatctaaa
    10501 ataattaatt caggatcttt aatcaaacat cttgctatgg aaatacgttg cttttgtcca
    10561 cctgaaagca ttacaccacg ttccccaatt atagtagaat attgtttagg aaaatgcata
    10621 atatcatcat gcacacaagc tatttttgaa taatgttcta ccttgttttt atctgcttct
    10681 aaattgccaa ataaaatatt attttcaata gtatctgaga atagaaatac atcttgtggt
    10741 gtataagaaa gtagcgctct atatttattt agattgatgt tttttatatt ttcgtcatta
    10801 atcagtaatt cgcctttatt tacatcataa gtgcgacata acaattcggc taatgtagat
    10861 tttccacttg ctgtcctacc aacgattgct atcttttctc ctttttttat ttgaagattt
    10921 atgtttttaa gtgcatcaat tccagtatct ggatacgtaa aagaaacatt ttttaattca
    10981 atttttatta tatcttttat gattggcgat tgtgcagatt gtatgatgtt tttgtcgtct
    11041 aaaaattgat taattctttt ttgtgatgct gcggctcttt gaactaaaga aactgcccaa
    11101 cctaatgaag caacaggcca ggttagcata ttcacataat aaataaattc agcaatgttt
    11161 ccgactgtaa aattgccttt ttgtacttct ataccaccaa tataaataac cattaatgtg
    11221 cttaatccaa ctaaaagtaa catcaatgga gcaaagtaag cttcgatttt tgcaaggttg
    11281 atgttttttt gtttatatac ttcgcttgat gcagcaaatt cttcttgagt tagtttatcg
    11341 atggaaaatg cttttacaac ccgaattcca gaaaatattt cttgtgctaa tgatgtaatg
    11401 ttagataaag cagttgaaat ttcctcgctt ttggtattga taacagaact tactttataa
    11461 attagaaaag caagtatagg taaaggtaat aatacaaaaa ttgaaagata ggcgttggtg
    11521 gcaaccattg aataaatcac cattacaaat aatgcaatga tatttattga atacattata
    11581 gcgggaccaa aatacattcg aactcgagaa acatcttctg aaattctact cattaaatct
    11641 ccagtattgt ttcgtttata aaaagaagta tttagagttt ggtattgctt aaaaatttgg
    11701 tttttttggt catattcaat ttttcttgac atcacaatta atgtttgtcg catgaaaaac
    11761 atcaaaacgc ctttgattag tgaagcgata ataactaaaa ttgcaataaa caaaataaat
    11821 tgtccaaaaa ggtaatttct aaatattgct tgtgtttcag aatgtccaat aagttgatat
    11881 aactgaatat tgtcctttaa gatatcaatt ccttcttgta tatattgtgg aggtagtaat
    11941 ccaaaatagt tagaggtaat aacaaaaaat atgcctgata gaaaaagcca tttgtacttt
    12001 aaaaaataaa aatttaccgc tctaagttct ttcatgcaat tttacaaagg taagaaatat
    12061 tataaattct taattttttt aatatttaat aagattcgaa aaataaaaaa gggaaactat
    12121 aaaaagtttc cctaaataat atgaggaggt tatttgttaa aatgagaaaa ttgtcgccaa
    12181 cattaatcta tattgtaaat tacttccagc tccagttctg ctaagaaata cttttttgtc
    12241 agcataatct attctgaatt ctggtttgac gcttaggaaa tcactgatag tgatattgct
    12301 agtcaaagtt acatctataa aatgtttagc aggtccagca acgtacggaa tagctaaatc
    12361 attgttgtta gaatagccaa ttcttgcacc aattccaaca ttgtctttaa tattgtattt
    12421 gctgtataag taagttgtga aatattgtgc atgcgttgct ttgtcgcctt tgcttgcaat
    12481 ttgtctaaat ctatgccacg caaaatcaat agccatagag ccttttgatt tcttgcctaa
    12541 agttgcagat ccagtaaaac ctaatgatga tttataattt ttgaaggcat ctgttgtgtc
    12601 atgtaggttg ctttcgtttc cgcctacata attcaaataa gcactaaatt tgtcgccagt
    12661 gtaaccaact tgagacccaa caaacttgtt tctgtcttta tcatctttat gatctgtatc
    12721 attaaagaac ccaactaaaa agttaaactt gttttttgta aaatttgctt taaaacctgt
    12781 atgataatat ggtccatttt gaaatgcaag tgataatgaa taatggaagt ttgcggttgg
    12841 ttcaatatat tcataaccaa aaaaagtact aaagttgcca agtgttaatt ttacccattc
    12901 tgctggagca tacgtcatgt ataattgctt aatggcaata atcgagtttg cataaagtgt
    12961 gttgtttgca gcttcagctc ttggcccaaa tccaatatca gccataaagc ctactttttt
    13021 tatttcctta gatagcataa aatttatcat acctaactgg aatgaattgg ctttgggaga
    13081 aaatgcttca tttaaaacat cattgctttg ttttggaata aaattggttt gaacataagc
    13141 atctactgaa ccagaaaagt tcaatggaag tttagattgc catttgtcaa ttttttcacc
    13201 tacagatgtt ttagtagagt ccactgcttc ttgggctttt gtgttgaaac ttagaataag
    13261 taacgtagta cacactagaa tcattttttt catacgcata aagtttagtt attaattata
    13321 tgcaaggata ttgcatattt ttttaaaaat accgaaagtc aaattatgaa tttatttttt
    13381 aaaagttaat aaattttaac taattattgt ataaattatg aaattttata aaaataatta
    13441 aaatattttt gacaaaaatg ccaaagaatg caaaaattac taaaaataat taaaatattt
    13501 attctgctat tttttaaaac taatgctgaa tcttggacaa agaaatcttg tcattaataa
    13561 ttgacgggat aagaattttg tagaatttaa gaattatgca gtcgctaatt tctcttttat
    13621 tttagtttct atttcatcaa cgattgcagg gttgttagct aagatttgtt tgattaggtt
    13681 tactactttt tgcccttcgt agttttgtca gctttaatca tttttatttg ataatgtagg
    13741 gaatacttat ttatatttgt tttgtaaaat attttccata accaattaaa acagtattga
    13801 tatatcataa attctatgac agattaaaaa gatttgatta tttaacgata aatcaagtat
    13861 atttataagt aattaataat cttgttgcgt tatatctata acttgaatct gcaagaaatg
    13921 atgatactga cttttaaatg tataaagaaa tttataaata aaaactatta aaaacatatt
    13981 actatgaaca gaataaagga atatttacta ataatagtgt gtctattggg ttttcagatg
    14041 tatttaaaag caaatatggc atcgcctgat ttggaactat taagtggtgt atctactata
    14101 agtagccaac aaattgatat tttacatgag gatttgaaaa ttacccttga cccatttttt
    14161 gataaagcat ttttcgatgt gcattataga atcagagcgg ataagtcggg taagcaaatt
    14221 ccactacttt ttgtagctga gttttataaa tctaatttta cagtagaggt agatgggaaa
    14281 cctgttgcga tactccaaat tccagagact ttttatgaat caggaagatt gaaggagtat
    14341 agtccgccaa atatagaagt taaagaggac agagtatatg ttaattggcc agtattccaa
    14401 aaagattatg aaaaaaaaga tgttcaatac tttgaagttg atttgactaa aggagaacat
    14461 tctatagtta ttcgctatga atcgagttca cttacggatc aagaaaataa catagttacg
    14521 aaaaaatcct ttaagtactt gctttctcca gccaagcttt ggaaatcttt cggcggatta
    14581 agtttgacta tagatggcac tgacttttta aaagaaaatc ccagcacatc tatttctttc
    14641 gactttggag atttgacccc aagtgagcca atgaaacgat gggaatttga ttctatacct
    14701 gttgattatt tctatatcaa ctattatcca aaagttccaa aaacaattca accttatatt
    14761 gattactata aaccagcaat catcattttc ggaggaggta tgatactgtt gctgattatt
    14821 gcttatgtgg tttatagata cataaagaaa caaaaaacgc aagggtaact aaaatctcta
    14881 gaaaaaatat gactatcaaa acgcataaat ataagaaatt ggaattctta ttttagtttt
    14941 gctctgatag ccaatttctt aaattatatt agcaaaattt taattaaatt gtcgctaatt
    15001 tctcttttat ttttgtttct atttcatcag ccattgctgg gttgtctgct aagatttgtt
    15061 tgacttggtc tctaccttgc cctactttct gaccttcgta gctataccaa gaaccacttt
    15121 tttgtaaaac acctaaatca gcacctaaat caataatttc tccatctttt gaaacgcctt
    15181 ttccaaagaa aatatcaaat tcggcagttc tgaaaggtgg tgctaattta tttttgacaa
    15241 cttttatttt agtttggttg ccaataacat tgccgtcttt gtcttttatc tgtccagttc
    15301 ttcttatgtc taaacgaatt gaagcataaa attttaaggc attacctcca gttgttgttt
    15361 ctggactgcc aaacatcaca ccgattttat ctcttaattg gttgatgaaa atacaagaac
    15421 aacttgtttt gttgatggct cctgttagtt ttctcatcgc ttgagacatt aatcgagcat
    15481 gtaaacccat tttgctatca cccatatcgc cttcaatttc acttcgtgga actaatgcag
    15541 ctactgagtc aataacaacc aaatcaatgg cagcagaacg gattagttgc tctgcaatgt
    15601 ctaaggcttg ctctccatca tcaggctgag taatatataa gttatccgta tctacaccca
    15661 atgcttcagc atatttttta tcaaaagcat gttccgcatc tataatggca catatcccac
    15721 ctcgcttttg tgtttctgcg atggcatgaa ttgcaatagt tgtcttacca gaactttcag
    15781 gaccataaat ttctataatt cgacctcttg ggaatccacc tataccaagt gctaaatcta
    15841 aacctaaaga accagtagga attacatctg actcaacaat actgttgtct cccaatttca
    15901 ttacagttcc ttttccgtat tgcttatcta acttatctaa ggtaagttgc aatgctttta
    15961 atttgttttc atcttttact ttttcgctca taattgatag acgttttatt tttctatttt
    16021 ccacaaaata acaataattt tctgaaagtt tagaaatatt cttcaaatcc ttgtaagttg
    16081 gtataattgt aatataatgt gtacatttaa ctttatttta accgcatttc tataataata
    16141 tatgcatttt tgcgtaatat tattttttcc ttttggttaa ggaaatgttt agtaatttat
    16201 aatgagaaag cgacagacta gtatatttat tttagcacac tctatcggtg atattgtgat
    16261 tctaaattta tctattattt taggtcatat ttggagcttt tggggcaaac ctattcagat
    16321 tttttctgaa atgtatttgc aaatgtggtt atacttaaat ctaatttatt ttgtttcagc
    16381 acaaatttct gggacttttg atatgtatag aaatacacgt ttctttacgt tagtttcatc
    16441 aatcttcagg ttatttttct ttcaagtcgt ttttgctttt tcctatatcg ttatatttaa
    16501 agactttaat aatactttca aattatcgag agaggcattg ttggttactt atctatcctc
    16561 ttttatattg actttgattt ggcgatttgg atttattaaa atggctcgtt tttatagagc
    16621 acgaggtttt aataatcgaa gaattgtaat tgttggagct ggagaatctg ctcaagattt
    16681 taaaaagatg ctggagagta gagttgaata tggttataaa tttattggtt tttttgaaga
    16741 tagacctgaa cgctataatt taaaagtaag agaaaatata ttaggttcta ttgatgaagt
    16801 aaatgcttat gctttgaaca atgatattga tgaaattttt tgtgctctgc cttatagtga
    16861 agagataaaa attaaagatt taatttcatt tggagatgag aatttgatac gtgtaaagat
    16921 agtgccagat atgacaaggt tcttgtcgca tcatctaaat aagatagaaa ttgattatta
    16981 tggacaaatt ccagtaatga ctttaagacc tgagccgtta gaaaacttta tcaatagagt
    17041 tataaaaaga atttttgata ttggattttc agtcttgata attacattcg tgttgtcatg
    17101 gttaatacca atcatctcct tgataattaa actaacgtca aaaggtccag tttttttcaa
    17161 acaaaaacgc tcaggattta gaaatcaaac ttttgatgta attaagttca gaacaatgta
    17221 tgttcatgat gattcaaatg ccaaacaagc aactaaaggc gacagtagaa ttacaccaat
    17281 tggggcattt cttagaaaaa ctagtttaga tgaatttcca caatttatta atgtattttt
    17341 tgggcagatg tctgtgattg gtccaagacc acacatgttg aaacatacag atgaatattc
    17401 aaaaattatt gataaattta tggtgcgtca tttggtaaaa ccgggaatta caggttgggc
    17461 tcaagtgaac ggatatagag gagaaacccg aaacaaacaa aaaatggaag atagagtgag
    17521 agcagatgtt tggtatattg agaattggag ttttacttta gatattaaaa tagtttttat
    17581 gacgattgtc aatttgataa gaggtgaaga gaatgcattt tagatatata ttattagcga
    17641 ttttttattt taatgcattt ggcattacaa tatttgcaca acaagaaaat ctctatttga
    17701 atagagagaa atcattgact ttcgagaaaa agttgaacca aatggataag gcctcatttc
    17761 atagtgccat aaaaccatat attgcttcag aagtgtatga ttttgtatat cctgattcgg
    17821 ttatacatta tactaaactt caaaaatctg actggtttag caaaatggtc aatgtagttg
    17881 gatttgaaaa tttcttagag tttgatgaaa atggttatgt aaaatatcgt agagatattg
    17941 ttgcaaatcc atatttgcca acctataatg atgtcccttc tgatacagga tatgtaaata
    18001 gaaaattcta tgttgccatt aatccaatta ttaatattgg aatgggtgtg gatttttata
    18061 ctagtaaaat tatttcacaa agaacagttg ggtttgaatt aaaagcggat ataggaaaaa
    18121 aagtatctct ttatactgca ataacgaata atgttgtaaa gttcccatca tatattactt
    18181 tatattccgt tgcggcaaca gccgttcctg gagaaggtag aactagacct acatcgaaca
    18241 atagttttga ctattctagt gcgattgctt atttgtctta ttctcctgta aaacaattta
    18301 atatgcaaat tggaaatgga aagcacttca ttggagatgg cttccgttca ttattattat
    18361 cggataactc ttttaattat ccctatttac aatttacaac taatgtatgg cgaataaaat
    18421 ataccacaat ttataccgaa ttgttgaata acattaagcg tggtactgat tttacccttg
    18481 gttatcaaag aaaattagcc acatttaatt atctaagtgt tgatgctgca aaatggttgc
    18541 aattgggtgt ttttgaagga attacttgga gaagaacaac ggataaggga aatacatttt
    18601 ttgattataa tttcatcaat ccaattttgg gagtaagagc atttcaaaag aaattaaacc
    18661 caaacatcaa caaagtttat ggtacaaata tcaaagtaac tctgcctaaa aatattatct
    18721 tatatgggca atttgcggct caaagttggg gcaaaaaaaa tacggcaaag cgaaaaacag
    18781 catttcaagt tgggatgaaa tattttgagg ttggtaatgt gccaaatttg aacttacaag
    18841 tagagttcaa tcaagttcga ccttttatgt atcaaggctt agatagtttg ttgtcttata
    18901 gtaattacaa tcagtctatc acacacagta tgggtgctaa ctttagagaa tatttagcca
    18961 ccttaaacta tcaatggaaa agattatata cttcttataa atttaatttt actcaagcag
    19021 gatacgattt tacaggcaat ttcctgaata ttgaaaataa tggaggaaat attctaaagg
    19081 cagaaaaaga tattgtgaat tctatgaatg taaagatgtt taatggcaca ccttataata
    19141 ttattaataa cgaatttaga attggttata tcattaatcc taaaattaat tttgtagctg
    19201 aattaggtgt tcagttcaga aattactcta gttctagtaa tgactttact aatttttcaa
    19261 gccatagcat tggtctaagt tttaaaacaa atatttttaa tagatattac gatttgccag
    19321 tgttgttcta aaaaatcata aaaaataata aatgtagcaa tagattagat attattctta
    19381 tttttgttac tttacaaatc aaataaaaaa tatgcagaaa gtatttataa cgctattgtt
    19441 aggacttgtt tttttaagtt cttttgcaca agaacagact gtaacagcga caagtggtgt
    19501 ttctccagaa gtaaaagcaa atattaataa agcaaaagat agagtactaa tagaattagg
    19561 cttaaatcaa atgctaaaaa cgcctagtga tttaaaagtt agtgcattgt caagaagttt
    19621 taatgcttat tatacttatg atgttgtgtt cggaaaatca aattttagca tagcaccagg
    19681 agcaggaatt ggtactgata atttctttca taaaaaaaat ggaattgctt ggaagaacaa
    19741 tacgcactta aaagattcaa ttacatcttt tccattatta ggagatagtg ttagttctaa
    19801 aaaatcaaaa ttgggcttag tttattttga tattccagta gaatttagat atcgttctaa
    19861 gccaaacaag aaaaatacat cttggaaatt ggctgctgga gcaaaatttg gtttcttatt
    19921 ggcaagtaaa tggaaatata aaggcgaaga cccagcgact ggtactggag gggatgtgaa
    19981 atttaaagaa tttaaagttg atggattaaa caagtttaga tatggtgtgt atgtgcgtgg
    20041 aggttatgga atttttaatg tctatgtaaa ttatcaaatt tcatcagtgt ttcagaaagg
    20101 aaaaggacca caaatgtacc cattgcaatt tggtattgca atatgtgggt tatagtattt
    20161 taaagtattc ttaaagcaaa gaatatataa aattgcatca aagtttttgt aactttatat
    20221 tttaaattaa atccgatttt gagaatcgga ttttttatat agaaaaagaa aaaaggaatt
    20281 atggataagg tatatatttt tgacactaca cttagagacg gagagcaagt cccaggttgt
    20341 caattaaaca cagtagaaaa aattgaagtt gcaatagctt tagaaaattt gggtgtagat
    20401 gttttagaag caggttttcc aatatcttca cctggagatt ttaattctgt tgttgagatt
    20461 tctaaaaatg taagtaatcc tacaatttgt gcattaacac gtgcagtaga taaagatata
    20521 gaatgtgcag cagaagcatt gaaatttgca aaaaaaggaa ggatacatac aggaattgga
    20581 agttcagata ttcatattat aaataagctt cgtaccacta gagaaaatat tttagaaaaa
    20641 gcagcttggg ctgcaaagta tgctagaaat tttgttgatg atgtcgaatt tttctgtgag
    20701 gatgctggtc gtgcagattt agctttctta gcaaaaatgg tcgagacagt tattgctaat
    20761 ggtgcaacag tagttaatat tcctgatacc acaggctatt gtacgccaga ccaatatggt
    20821 gcaaaaattg cttacttaat gaataatgtt ccgaatattg ataaagctat tatttctgta
    20881 cattgtcata atgatttagg attggcaaca gctaattcaa tcgctggttt atcaaatggt
    20941 gctagacaag tggaggtaac gatgaatggt atcggagaac gtgccggaaa tacctctttg
    21001 gaggaagttg ctatgattat caaatcgcat aaagaatctt taaaccttta tactgatatc
    21061 aatgtaaaaa acatttaccc aacttctaga ctaattacta gattgatgcg tatgccagtt
    21121 cagccaaaca aagcaattgt tggacgaaat gcttttgcac attcctcagg aatacatcaa
    21181 gatggcgtac taaaaaatag agagaattac gaaattattg acccaaaaga tgttggagtt
    21241 cctgaatctt taattttatt gacagccaga agcggtagag cagctttgaa gcatagatta
    21301 gatttattag gttatcattt agattctgaa aaattagata atgtttatca agaatttttg
    21361 aaagtagcag a
//
LOCUS       DAOQCW010000031        20975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_915_length_20975_cov_5.278585, whole genome shotgun sequence.
ACCESSION   DAOQCW010000031 DAOQCW010000000
VERSION     DAOQCW010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 20975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20975
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_915_length_20975_cov_5.278585"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1329
                     /locus_tag="PLS16_04260"
     CDS             <1..1329
                     /locus_tag="PLS16_04260"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNJ60328.1"
                     /translation="SFKTKLLFCCGQVGWSLTIFGFAELIYMFYLPPDNGVPLFKSYI
                     YQGSILQIFTIIGILTAVGYIISAFMEPIVAAWSDRNNFKFGKRKTIMAIGVLPFAIS
                     AALVFFPITEYVSTINAIWVIVTTLIAFSVKCLYTTPYNAMINEVGTTEKDRLHLVML
                     LSVTYGIGIGLGNLIHIIIKVSEGVLTHERAFQYTILAYSTLAFILMMIPILFVEDNS
                     NKKQSNQKPVGAFEMMHTVWQNKNFRTFAFTELVYWFPSKIFTVAIPYFVTALMQLDN
                     YYTSIILYACGIGSFATYPIIDKLVAKYGKKKILQSAFLGLAISSLFTATIGLYNLPT
                     GLFIAIYILLNTYPTAVLGILPMSLTSDIAEEDFQKTGVSKNASYYGFKTFMMKIGVA
                     ITSLIFPSLLLLGKTPENPLGVRMISIACIITSLLAFFIFRKFKEIEKLA"
     gene            1326..1991
                     /locus_tag="PLS16_04265"
     CDS             1326..1991
                     /locus_tag="PLS16_04265"
                     /inference="COORDINATES: protein motif:HMM:NF025086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HNJ60329.1"
                     /translation="MKILNHILLFVLILYIIPAKAQSKTTVKIGLMKYKGGGDWYANP
                     TSLSNLAKFCNQNLGMNLNPKIDEIEPGNADIANYALIHITGHGNIIFSAFEAENLRN
                     YLIGGGFLHISDNYGLDKYIRKAMKTVFPELEFTLVPFNHPIYHQKYDFPNGVPKIHE
                     HDGKAAQGFGLFWQGRLVCFYDYETDLGDGWEDPEVHNDSEDVRQKALKMGANIVQYA
                     LTY"
     gene            complement(2013..2252)
                     /locus_tag="PLS16_04270"
     CDS             complement(2013..2252)
                     /locus_tag="PLS16_04270"
                     /inference="COORDINATES: protein motif:HMM:NF015582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HNJ60330.1"
                     /translation="MQKSLLFTYIILIVLTILSPLLATYVVVKPIIYIIIIFSVIKFL
                     LVGYQFMELKNAHTFWKAIFTIYACLIGGILMILL"
     gene            complement(2255..2845)
                     /locus_tag="PLS16_04275"
     CDS             complement(2255..2845)
                     /locus_tag="PLS16_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNJ60331.1"
                     /translation="MNTTDIEQNNPKLLSNPPGGLLIWIVIYLELLTYGLAFFSLAYY
                     GSIEKDLFHKHSQLLDKPIAVVNTIILLTSGFLIAKGVHFFKYKQNQKSGRYFTLAML
                     FGLTFLVLKMIEYKGKINAGLDMDYSTFFMFYWLMTGFHWVHVFVGIIILFFVRKMIV
                     KNDSNADVLDVEAGAAFWHLCDLIWLILFPMLYLLF"
     gene            complement(2851..4611)
                     /locus_tag="PLS16_04280"
     CDS             complement(2851..4611)
                     /locus_tag="PLS16_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNJ60332.1"
                     /translation="MEIDEIIYSKVLRFFKNRKENNAEVLNRKVLLSDLKPRLTLIAR
                     AICGTAIEIFPAEREGGFKNDNFFLPISVALFQSKEENIQFYIFRTIYLCVQKQLEFN
                     WTETIYSEELSSVKAIETAPDVLKKMFEDYPSLQDNFEVLFNQLPIDKKTQKPDVYWL
                     YGKWMKNTQEENNADKLQNFDNQANQLINKIEAKTTLHTKAVEEIESLTIDKKQQEDY
                     VLMHSFEKIETAEEFNGNWRDFDGEDDLKDHQEALEEMNMKFTVRVDDTVHSVYQADF
                     VENTSVSESSEIDEKGFHLKYDEWDFKNRKYLVDYCKVYPKSQVKTDTIYYKNTLTKY
                     RTILVSLRKMLTSVNNKMQQQRRQIQGELFDLDALTDLYTAIHSKVSPDERIYLSQRK
                     KDKDIAILLLLDISLSSDGYAAGKRIIDVEKDVSILFGEILNEFNIEFSIDGFSSKTR
                     NFSTYITLKDFNEDWNKAKYKIGAVEPNGYTRIGPALRHAGARLSKTEAKNKWIILLS
                     DGKPNDYDRYEGQHGINDVKQALRELNEAKINSYALAIEAQAKYYLPQMFGQNHYQIL
                     TSPEELIKSLAKLYEKIKHN"
     gene            complement(4713..5489)
                     /locus_tag="PLS16_04285"
     CDS             complement(4713..5489)
                     /locus_tag="PLS16_04285"
                     /inference="COORDINATES: protein motif:HMM:NF019346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HNJ60333.1"
                     /translation="MVENIFYKAIGSEVEVFNQANQLKLPLLLKGPTGTGKSRFVEFM
                     ANDLGKVLITVNCNEETSSTDLIGRFIIKGAETVWIDGPLSIAVKNGYILYLDEIAEA
                     RPDVIVAIHSLTDHRRELFIDKLGETIKAHPNFLLVASFNPGYQKGFKELKPSTRQRF
                     VSLSFEYPEPKVETEILITETGINKDIAQKLVNIGSKIRNLTELGLTETVSTRLLVNA
                     AKMIHAGLPKRLSVHVAVVEPLTDELETVKALKDLCDLMI"
     gene            complement(5602..6942)
                     /locus_tag="PLS16_04290"
     CDS             complement(5602..6942)
                     /locus_tag="PLS16_04290"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNJ60334.1"
                     /translation="MKYKSQKVAYWFFALCMLLFTLQIVYGFIMGFARIGMDGLHDFI
                     PFNTARAVHTNLLVVWLLTGFMGAAYYIIPEEAQTELVSVKWAYVQLVSLAVVGVVAI
                     VGYHFNYWEGRKFLEIPRELDYLVVVNVLLFLGLILATLYKGKRRTTTALVLTMGLVF
                     AALLYLPGMIWFDSQVTDSFFRWWVVHLWVEGVWELIMGGILAYLLIKLTGVDREVIE
                     KWLYVIVGLTFLSGILGTGHHYYYIGVNKIWLVVGGIFSALEPLAFLAMALFAMYMYR
                     KGEKSHPNKIALFWTIGASIVSFIGAGLLGFAHTLPQTNIYTHGTLVTAMHGHYAFWG
                     AYAMIVLAIISYALPNMTGRKRYNFDAGHYAFWLANIGMLGMTVAFGVAGVAQVYLER
                     IMKLDFMDVQNEISIHFIVLLLSATMFATGIVLYIIEFAKHGQPTDEALNMVEE"
     gene            complement(6967..7395)
                     /locus_tag="PLS16_04295"
     CDS             complement(6967..7395)
                     /locus_tag="PLS16_04295"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNJ60335.1"
                     /translation="MLSKSQARTFFLGGTLVTFLVFIALTVYSFQPKNDQTNHDKIDA
                     KVIRGKEIFEANNCMGCHTIMGEGGYYAPELTKVIDRRGEAFVKAVLQSPVPWGSRGR
                     KMVKYEMNDVDADAMVAYLKWIGELDLNGFERVVSPLSKD"
     gene            complement(7700..8092)
                     /locus_tag="PLS16_04300"
     CDS             complement(7700..8092)
                     /locus_tag="PLS16_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60336.1"
                     /translation="MLVLTVLLVAVILNLVFFLMKKPYHKLAKISALVGLIFTHLQIV
                     LGLVLYFVSPVGFSNFSGEAMKETTSRLYLVEHPIGMIVAAILVTIGYKATKNMSLNN
                     NQKYTKVLINYLLALAIVSYLIPWFVWK"
     gene            8300..9046
                     /locus_tag="PLS16_04305"
     CDS             8300..9046
                     /locus_tag="PLS16_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60337.1"
                     /translation="MENKIEYQKLQHLLNTESKDFEFLQKLSADELRTLRVRMTEVMD
                     LDQPEIWGRVAKVAKFMPNFLNAKVSETVLGALITANISTYVPIKDAISIMKNLSTPF
                     LAEVAEYMIPEKSEELIENISVDTLKKITTQLLKNKKYYVASGFVNVLSFPKINAISE
                     VITDELDLILIAEFVENKAHIARIVEGFSDKRILKIIQTAYKNNKQEEILTVFKLLSP
                     RELTRVLSIVKQLSDTEQNTILQDFVTRIK"
     gene            9049..10680
                     /locus_tag="PLS16_04310"
     CDS             9049..10680
                     /locus_tag="PLS16_04310"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNJ60338.1"
                     /translation="MEQEQTLFESYKKRPKLDEHYDVICIGSGLGSLCTASLLARNGK
                     KVLVCEKHYTPGGYTHVFTRNDYEWDVGLHYVGDMDRKGSALRIIFEYITDRNLKWAD
                     MGEVYDRVLINGKTFEYLKGLSKWKERMKIYFPTEQDAIAIDKYVEILFAVNNAGKSF
                     YMQKAMPKLVQFFTKPFLNKSYQKYYSQTTKELLDSLSDNEELKAVLAAQFGDYGMPP
                     SQSSFVIHAAVALHYMNGGFYPVGGSEEIFNTIMPSILNTGGKTIISAEVKEILVENG
                     KAIGVLMADGKKITADIIVSGVGAHLTFEKLLNTENQKKIKFSNDLKNLPPSYGHFSL
                     YIGLQHTATELNLPKANYWIYPNGNDHDAAVEKFMNDYNEEFPVVYISFPSAKNPKFD
                     EKYPGRATIEIISVANYDWFTEWENSRWKKRGENYETFKEKIAQRLLAHLYKVEPQVE
                     GKIDTYELSTPLSTKNFCSYQKGEIYGLTHSPQRFDSEYLKPQTPIKNLYLTGQDIVT
                     VGIAGALMSGVLTTSAILGKNIMNDIIQYAKSTPQ"
     gene            complement(10683..11843)
                     /locus_tag="PLS16_04315"
     CDS             complement(10683..11843)
                     /locus_tag="PLS16_04315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNJ60339.1"
                     /translation="MKEKIKLLILNKYLLFLLGIIIVGFSIWYTNQLASVIKKQEIKQ
                     ANDLANAYHILISGDGGNLDDALEIIKINDNIPIIWSDSQNNILAYKNFDSTKVEADA
                     SYLKQQIHKLKNKKIVIHIDENQTHFLYYKDSILLQKIKKYPYYQLFLVILFFSISFI
                     AFASIRKAEQNQVWVGLAKETAHQLGTPLTSISAWVEILKDRLNDDVESMVMLEEMDK
                     DLDRLDLVAKRFSKIGSPPELKVELLLPIVENVVEYMKKRAAYSIVFQIKDTTNREAK
                     VLINQQLFEWVLENLIKNALDAMERKGKIELIIFEDKNLVYIDVKDSGKGIPSGNFET
                     VFNTGYSTKKRGWGLGLALSRRIIQTYHKGKLFVKDSVIDKGTTFRIILKQG"
     gene            12011..12085
                     /locus_tag="PLS16_04320"
     tRNA            12011..12085
                     /locus_tag="PLS16_04320"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12046..12048,aa:Val,seq:tac)
     gene            complement(12148..12798)
                     /gene="aat"
                     /locus_tag="PLS16_04325"
     CDS             complement(12148..12798)
                     /gene="aat"
                     /locus_tag="PLS16_04325"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HNJ60340.1"
                     /translation="MGLVVLDKSIWFPPVENALDEGILAIGGDLSEERLLLAYSSGIF
                     PWFSKDEPIAWWSPDPRFVLFSEDLYVSKSMRLLLNKNKFKVTTNQNFKEVIDECANA
                     KRNFQDGTWITDEMIDAYINLHKKGYAHSVEVWNGDELVGGLYGIKMGKCFFGESMFH
                     KMDNASKYGFITYVNQLKSEGAVIIDCQVYTPHLSSLGAIHIPRKKFVEIIKANCK"
     gene            complement(12803..13111)
                     /locus_tag="PLS16_04330"
     CDS             complement(12803..13111)
                     /locus_tag="PLS16_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HNJ60341.1"
                     /translation="MDFNQFFFHTQTKENPDTDVLEMEETDYGWQIIVHNDDVNTFEW
                     VIESLVAICQHTYEQAEQCALFIHFKGKYAVKHGTEEELLPMKDALLDRGITATLESV
                     "
     gene            13190..13263
                     /locus_tag="PLS16_04335"
     tRNA            13190..13263
                     /locus_tag="PLS16_04335"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13224..13226,aa:Met,seq:cat)
     gene            complement(13325..14233)
                     /locus_tag="PLS16_04340"
     CDS             complement(13325..14233)
                     /locus_tag="PLS16_04340"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNJ60342.1"
                     /translation="MDKQVFHLTSQKQKGIFFVIIGAICFSSKGVLAKLAYRYGVSSL
                     DVLNLRMLFSLPFYLFIYFRETKRKPIAIKPSIRWDIVLLGLIGYYLAALFDFKGLEY
                     VSANLERITIFIYPTFVLLLGFIFYRRKVSLVQLISVLICYVGIVLAFYADIDSMDFK
                     KGFIGVVFVLLSALTYAFYLVRSDNIIKNIGTLRFTCLSMMVSCIAVLIHNFLVNGFN
                     LLSFSMNIYIICGIIAIGATVLPSFLMTYGISLIGSSNMSIIASIGPVSTMFLAYYIL
                     GESMTWMHILGTILVLLGIYILSKRS"
     gene            complement(14261..15208)
                     /locus_tag="PLS16_04345"
     CDS             complement(14261..15208)
                     /locus_tag="PLS16_04345"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HNJ60343.1"
                     /translation="MKIGDRIRVYAPATVANVACGFDILGFAIEKPGDELIMEITAEK
                     GVVIVDIEGDGGVLPRDAKKNSATVAIQDYLNYINADFGCKIWLKKMMPSGSGLGSSA
                     ASAVAGVYAINMLCKEQLTKNEMLPFLINAEKAACDAAIADNVAASLFGGFILVRSYE
                     PLDIIKIPVPEELWCVVINPDVIVLTKEAREILPKEIPLTHALRQSANVGGLMIGLLR
                     GDYDLIGRSLIDYIAEPHRSKLIPGFYDMKNAALESGALGSSISGSGPSVFALCKGQE
                     IANKVGDAMKQVMNNLGIGAEVYISKVNAQGPKVLPFLD"
     gene            complement(15226..17688)
                     /gene="thrA"
                     /locus_tag="PLS16_04350"
     CDS             complement(15226..17688)
                     /gene="thrA"
                     /locus_tag="PLS16_04350"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF006959.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HNJ60344.1"
                     /translation="MKILKFGGSSVGSVENIKRIIDIVKNEKKQGNDVILVCSAFKKI
                     TDQLINAGKLAEKRDDNYKAVLENIKERHLAAIHALVSKKSIKPALLEELEEGLQRVD
                     DILHGVSLLAELSDKTLARLMSFGERLSCFIIAGALTEVNLKGVYVNATQLIKTDSNY
                     VAAKVNFDRTNKNILEYFEHLHGIPVITGFIGTDEFNDITTLGRGGSDYTAAIFGAAL
                     KVSAIEIWSDVDGVLTADPRRVDQAMTVPALSYKEAMELSHFGAKVIYPPTIQPAYVK
                     GIPIYIKNTFNPEHPGTFISKNSGKSVNMIKGISSVSDIALLRMEGTGMVGVVGIASR
                     LFGAFSKAGINIMFLTQGSSEHSICFGVLPNDVKKCRKALEDEFRYEMQNKFIQPLVI
                     ELENSIIAVIGENMNNVPGVSAKMFRALGKNGINVKAIAQGSSEVNITAIVDKYNEAK
                     ALNALHEIFFEQDKLSINVFLVGYTGLIGGTLLKQISHQKSYLENEKNLEINLIGVIN
                     SKNMLIDSNGIDIENLESKLKVEREDANINNFINRAIDLNFANTVIIDCSANKSVVEH
                     YERILKKSISIVTPNKIANTLAYPKYLSLRETAKRANSRFMYETNVGAGLPVINTLHA
                     LLNSGDRIIKIEAVLSGTLSYIFNSYKGDILFSDVVKEAKTKGFTEPDPRDDLSGLDV
                     ARKALILARECGAKLELEQIKVENLVSKELRDIDVKAFLQKLTDLDAVYDKLKKKAEK
                     KGNALRYMAVIENNKVEIALKEVDAQHPFYNLSGSDNMIVFTTERYKNNPLVIKGPGA
                     GAEVTAAGVFSEIISLGNYLAN"
     gene            17806..18567
                     /locus_tag="PLS16_04355"
     CDS             17806..18567
                     /locus_tag="PLS16_04355"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNJ60345.1"
                     /translation="MLKLLHGIYTLWLSLTFFIMTPLMLFCFLMIKLFIPYPKQIQFV
                     YYVNRFFFFFWSAINGIRYKITGLENINKDQTYICVLNHTNSGDMVAGAYGCRVNAKP
                     LIKRELTLIPFLGQMLSIACIAVDRSSKEARKKSKENMLNDLKQKISVLIFAEGTRNR
                     TGKPLKDFFDGAFDLAIDAQVPIIPIVLTNIKKINPKHDWFFRPTTIEVRHLAPIFPE
                     GKTEECIQKLKAKTFKNMWNCLVEFDDEFKKYDKL"
     gene            18571..19254
                     /locus_tag="PLS16_04360"
     CDS             18571..19254
                     /locus_tag="PLS16_04360"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNJ60346.1"
                     /translation="MYGICKLTAIPMRQEPSDKSEMINQVIYGETFDILQKDEKWSKI
                     KLHHDEYEGWIDNKQYEKSTKKASPKNINNKKYISKEHKIIPLGAFINFEINTSKKSI
                     IQTAKSLLNTPYLWGGRTCFGIDCSGFTQIIFRVHHILIPRDAYQQAEIGEFVQLKDT
                     QNNDLAFFSNPSGRIIHVGIIIRNKKGISIIHASGKVRIDQLDEKGIFNQEINAYSHY
                     LHSIKRITK"
     gene            19342..>20975
                     /locus_tag="PLS16_04365"
     CDS             19342..>20975
                     /locus_tag="PLS16_04365"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNJ60347.1"
                     /translation="MKIVIIGGVAGGATAATRLRRLSEENEVIIFEKGQYVSFANCGL
                     PYHISGTIEERNALLLQTPESLKERYNLDTRVFSEVTNINIEEKKVIVKNLQTDEIYE
                     ETYDKLLLSPGAEPIRPPFEGINSDKIYTLRNIPDMDRIVQKTKDAQNFVVVGGGFIG
                     LEVAENLIEAGKNVKLIELGNQVMAPVDYDIASYIHHKAKEKGLELLLNVGVQKFEDH
                     KDSITVFLNNDTSIETDAVILAIGVKPETQLAKNVGLMIGETGGILVNGFMQTSNPDI
                     YAVGDAVEVEHFIHQRKVLIPLAWPANRQGRLVADNITFGNKNKYSGSLGSSILKFFE
                     LSIASTGLNEKFLKRNNIPHKTAIVTRGHHAGYYPGAKNITLKLIFNDEGKIYGAQAV
                     GEKGIDKRIDVIATAIKGNLTVFDLPEIEITYAPPFNSAKDPVNIVGYTAENMLNGNL
                     EMINYDDLFQYQEDKNAILLDVRTPTEFARGSIKGSINLNVDDLRENLTQLDKNRNYV
                     IYCQVGLRGYLAYKILKNNGFKVVNLNGGYGLWSMI"
BASE COUNT         7457 a         3531 c         2889 g         7098 t
ORIGIN      
        1 tcttttaaaa ctaaattact tttttgttgc ggacaagtag gttggagttt gacaatcttt
       61 gggtttgctg agttaattta tatgttttat ttgccaccag acaatggtgt tcctttattc
      121 aaatcttata tataccaagg ttctatttta caaatattta ctataatcgg tattcttacc
      181 gcagttggat atattatttc tgcttttatg gaaccaatag ttgctgcgtg gagtgataga
      241 aataatttta aatttggaaa acgcaaaacc attatggcta ttggtgttct gccatttgca
      301 attagtgcag cattagtttt ttttccaatt actgaatatg tttctactat caatgcaatt
      361 tgggtaatag ttactacgct cattgcattt tcagtaaaat gtctatatac cacgccttac
      421 aatgctatga ttaatgaagt ggggacaaca gaaaaagata gattacattt ggtgatgtta
      481 ctttctgtta cttatggaat tggcattggt ttgggtaatt taattcatat aataataaaa
      541 gtctctgaag gtgtacttac acatgaaaga gcattccaat atactatttt agcctattca
      601 acattagctt ttatattgat gatgattcca attctatttg ttgaagacaa ttcaaataaa
      661 aaacaatcta atcaaaaacc tgttggtgct tttgaaatga tgcacaccgt ttggcaaaat
      721 aaaaacttta gaacttttgc ttttactgaa ttagtgtatt ggtttccatc aaaaattttc
      781 acagttgcta ttccctattt tgtaactgcc ttaatgcaat tggataatta ttatacttca
      841 ataatcctat atgcttgtgg aattggctca ttcgccacct atccaatcat agataaacta
      901 gttgcaaaat atggtaaaaa gaaaattttg caatctgcat ttttgggttt agccatcagt
      961 tcattattta ccgcaactat tggattgtac aacttaccaa ctggcttatt tatagctata
     1021 tatatattat taaacactta tcctactgca gttttgggta ttttgcctat gtcattaacc
     1081 agtgatattg ctgaagaaga ttttcaaaaa actggcgttt caaaaaatgc ttcctactat
     1141 ggatttaaaa cttttatgat gaaaattgga gttgcgatta caagtttaat atttccatct
     1201 ttattattat taggcaaaac acctgaaaat cctttgggtg tacgtatgat ttctattgct
     1261 tgtattataa cttcattatt agcatttttt atatttagaa aattcaaaga aattgaaaaa
     1321 ctagcatgaa aattttaaat catatacttc tttttgtatt gattttatat atcattcctg
     1381 ctaaagctca gtctaagaca actgtaaaaa ttggattaat gaaatacaaa ggtggaggag
     1441 actggtatgc aaatcctact tccctatcta atttggccaa attttgtaat caaaatttag
     1501 gcatgaacct taatccaaaa attgatgaaa tagagccggg aaatgctgat attgcaaatt
     1561 atgctttaat acatattacg ggtcatggca atatcatttt ctcagcgttt gaagctgaaa
     1621 atttaagaaa ctatttaatt ggtggtggat ttttacatat ttccgacaat tatggactag
     1681 ataaatatat ccgcaaggca atgaaaaccg tatttcctga gttggaattt acattagttc
     1741 cattcaatca tcctatctat catcaaaaat atgattttcc aaacggtgtt cctaaaatac
     1801 atgaacacga tggcaaagca gctcaaggtt ttgggttatt ttggcaaggg cgtttagttt
     1861 gtttctatga ttatgaaaca gatttaggcg atggctggga agaccctgaa gtccacaatg
     1921 actccgaaga cgtacgtcaa aaagcattaa aaatgggtgc taatattgtg caatatgctt
     1981 tgacttatta gctattaaat attgtaccat tattacaata atatcatcaa gatgcctcca
     2041 attaagcaag cataaatcgt aaaaatggct ttccaaaaag tatgtgcatt ttttagttcc
     2101 ataaattgat aacctaccaa taaaaactta ataacagaaa aaataattat tatataaatg
     2161 attggtttta ctactacata agttgccaaa agtggtgata aaattgtcaa tacaatcaaa
     2221 ataatatagg taaataaaag tgatttttgc ataattagaa taataaataa agcataggaa
     2281 ataatatcaa ccaaatcaaa tcgcacaagt gccaaaaagc tgcacctgct tccacatcaa
     2341 gcacatctgc atttgaatca tttttaacaa tcatttttcg aacaaaaaat aagatgatta
     2401 tacctacaaa aacatgcacc caatgaaaac ccgtcattaa ccaataaaac ataaaaaaag
     2461 tactataatc catatccaga ccagcattga ttttaccttt atattctatc atttttaaaa
     2521 ctaaaaatgt caatccaaac aacattgcca aagtgaaata tctacctgat ttttgatttt
     2581 gtttatattt aaaaaaatga acaccttttg ctattaaaaa tccactagtt aataagataa
     2641 ttgtattcac tacagcaatt ggcttatcta ataattggct gtgtttatgg aataaatctt
     2701 tttcaatact tccgtaatat gctaatgaaa aaaatgccaa accataagtc agcaattcta
     2761 aataaattac tatccaaatt aataagccac ctggtggatt ggatagtaat tttggattat
     2821 tttgttcaat atctgttgtg ttcattaaat ctaattgtgt tttatttttt catataattt
     2881 tgcaagagat ttaatcaatt cttcaggaga agtcaatatt tggtagtggt tttgtccaaa
     2941 catttgaggt aaataatatt ttgcttgtgc ttcaatagct aaagcataag aattaatttt
     3001 agcttcattt aattcacgta aagcttgttt tacatcattt attccatgtt gtccttcata
     3061 acggtcatag tcatttggtt tcccgtccga aagtaaaata atccatttat ttttggcttc
     3121 tgtttttgat aatcttgcac ctgcatgtcg caaagctggt ccaattctgg tataaccatt
     3181 cggttcaact gctccaattt tatattttgc tttattccaa tcttcattaa aatctttaag
     3241 tgtaatataa gtggaaaaat ttctagtttt agaagaaaat ccatcaatcg aaaattcaat
     3301 attaaactca ttcaatatct cgccaaacaa aatagaaaca tctttttcta catcaataat
     3361 acgtttgcct gctgcatagc catcactcga caaactaata tccaataata ataagatggc
     3421 gatatcttta tccttttttc gttgagataa ataaatacgt tcatcgggcg atacttttga
     3481 atgaatggct gtatataaat cggtcaatgc atctaaatca aataattcac cttgtatttg
     3541 tctgcgttgt tgctgcattt tattatttac actcgtgagc atttttctta ggctgactaa
     3601 aattgtacga tatttcgtta aggtattttt gtaataaatt gtatctgttt tgacttggga
     3661 tttgggatag actttacaat aatccactaa gtattttctg tttttaaaat cccattcatc
     3721 atattttaaa tgaaagcctt tttcgtcaat ttctgaactt tcagatacag aggtattttc
     3781 cacaaaatcc gcttgataca cagaatgaac agtatcatca acacggactg taaatttcat
     3841 attcatctct tccaatgctt cttgatgatc ttttaaatca tcttctccat caaaatcacg
     3901 ccaattacca ttaaattcct ctgctgtttc aattttttca aaactatgca ttaatacata
     3961 gtcttcctgt tgttttttat ctattgtaag cgattctatt tcttcaactg cttttgtatg
     4021 taaagtagtt tttgcctcaa ttttattaat taattgattg gcttgattat caaaattctg
     4081 taatttatca gcgttatttt cttcttgtgt atttttcatc cacttgccat acaaccaata
     4141 aacatcaggc ttttgcgttt ttttatcaat tggtaattga ttaaaaagta cttcaaaatt
     4201 atcttgtaat gatggataat cttcaaacat tttttttaga acatcaggag ccgtttcaat
     4261 tgcttttact gacgacaatt cttcggaata aatcgtttct gtccaattaa attctaattg
     4321 cttttgaacg cacaaataaa tagttctaaa aatataaaat tgtatgtttt cctcttttga
     4381 ttgaaataag gcaactgata taggcaaaaa gaaattgtca tttttaaaac caccttccct
     4441 ttctgctgga aatatttcta tagctgtacc acaaatagct cgtgcaatca atgtcaatcg
     4501 aggttttaaa tcagaaagca atactttacg atttaatact tctgcattat tttcctttct
     4561 atttttaaag aaccgtaaga ccttactata tattatttca tcaatttcca ttttggtgtg
     4621 ttgttataaa ttttacagct taattttttg gaaatttcgt gtaaactgcc acagtacaca
     4681 agcctctata taaaattaat tgtctaaatt tcctaaatca tcaaatcaca caaatctttc
     4741 aatgctttta ctgtttccaa ttcatcagtt aatggctcca caacagcaac atgaaccgat
     4801 aagcgttttg gcaaaccagc atgaatcatt ttggcagcat tcactaataa acgagttgaa
     4861 actgtttctg tcaaacctaa ctcagtcaga tttctaattt tacttcctat atttactaat
     4921 ttctgagcaa tatctttatt gataccagtt tcagtaatta aaatttcagt ttcaactttt
     4981 ggttcaggat attcaaacga aagcgaaaca aatcgttgcc gagttgatgg ttttaattct
     5041 ttaaaacctt tttgataacc tggattaaaa gatgcgacca ataaaaaatt aggatgagct
     5101 ttaattgttt cgcccaattt gtcaatgaac aactctcttc tatgatctgt cagtgaatgt
     5161 attgctacaa ttacatctgg acgagcttct gcaatttcat ctaagtacaa aatataacca
     5221 tttttcactg caatagaaag tggaccatct atccaaactg tttcagctcc ttttattata
     5281 aaacgaccaa ttaaatcagt tgatgaagtt tcttcattac aatttacagt aattaaaact
     5341 ttgcctaaat cattagccat aaattcaaca aaacgagatt ttcctgttcc cgttggacct
     5401 tttaataaca aaggtaattt caattgattt gcttgattaa acacttcaac ttccgaacct
     5461 atagctttgt aaaaaatatt ctctaccatt accataacct atttacttta aaatgataaa
     5521 tccaaaacag aagaagacaa tctaaagttt catgtcttct tctattaagg ataaactaaa
     5581 ctaatctaaa aactatcaaa attattcctc gaccatattt aatgcctcat cagtgggttg
     5641 tccatgctta gcaaactcta tgatatataa tactatacca gttgcaaaca tagtcgcaga
     5701 taataataaa actataaagt ggatactaat ctcgttttga acatccataa aatctaattt
     5761 catgatacgt tctaaataaa cttgagcaac tccagccaca ccaaaagcta ctgtcattcc
     5821 taacattccg atattcgcca accaaaatgc atagtgtcca gcatcaaaat tatatctttt
     5881 tctacctgtc atatttggca atgcataact gatgattgct aaaacaatca tagcgtaagc
     5941 tccccaaaac gcataatgtc cgtgcattgc agtaactaaa gtaccgtgag tatatatatt
     6001 tgtttgtggc aaagtatgag caaaacctaa taaaccagct ccaataaatg aaacaattga
     6061 agcaccgata gtccaaaata atgcaatttt atttggatga gatttttcac cttttctata
     6121 catatacata gcaaatagtg ccattgctaa gaaagccaaa ggctcaagag cagagaaaat
     6181 tccaccaact actaaccaaa ttttatttac gccaatataa taatagtggt gtccagttcc
     6241 taaaattcct gataagaatg ttaaaccaac aattacatat aaccattttt caataacttc
     6301 tctatctact ccagttaatt taattaataa ataagctaaa ataccaccca taattaattc
     6361 ccatacacct tcaacccata agtgaacaac ccaccatcta aagaatgaat ctgttacttg
     6421 gctatcaaac caaatcatac ctggtaaata caataaagca gcaaaaacca atcccattgt
     6481 taaaaccaaa gccgttgtag tgcgacgttt acctttgtat aaagttgcta aaatcagtcc
     6541 taagaatagc aatacattga ctaccaccaa gtaatctaat tctcttggaa tttccaaaaa
     6601 cttacgacct tcccaatagt taaagtgata accaacaatt gcaacaacgc caacaacagc
     6661 gagtgaaacc aattgaacat aggcccattt tacgcttact aattccgttt gtgcttcttc
     6721 aggaataata taataagcag ctcccataaa tcctgttagt aaccaaacga ctaatagatt
     6781 ggtgtgtaca gctcttgctg tgttaaatgg aataaaatcg tgaagaccat ccattccaat
     6841 gcgggcaaag cccataataa aaccatatac gatttgcaat gtaaacaaca gcatacacaa
     6901 tgcaaaaaac caataggcta ctttctgtga tttgtatttc ataactaata aattttaaag
     6961 gttattttaa tcttttgaca atggagatac tactcgctca aatccattta agtctaattc
     7021 tccaatccat tttaggtaag ctaccatagc atctgcatca acgtcattca tttcatactt
     7081 taccattttt ctacctcttg aaccccaagg aactggagat tgtaatacag ccttaacaaa
     7141 agcctctcct cttctatcaa ttactttggt taattctggt gcgtaatagc ctccttctcc
     7201 cataatagta tggcaaccca tacaattatt ggcttcaaaa atttcttttc cacggatcac
     7261 ttttgcgtca atcttatcgt ggttagtctg gtcattttta ggttgaaatg agtaaactgt
     7321 tagtgcaata aagactaaga aggttactaa agttccacct aaaaagaatg ttcttgcttg
     7381 cgattttgat aacataattt ctaaatttag ttagtgttta aaataatacc tttttacagt
     7441 gtaaaagtag aacacttttc tagacttgac tataatataa gtcagagtaa aatatgatta
     7501 aaatcattta attaatacaa ctaatatgcg tacagttata aaaaaaaata aaaaattagg
     7561 aaatattgga tggtaaaaat cctaaaataa ataaatccta aaaatcgaaa tattattaat
     7621 aaaaaaaaca gcatttactt atataagcaa atgctgtttt ttctatatag agttatatta
     7681 taaactaaat tatactagct tatttccaaa caaaccaagg aattaaataa cttacaatag
     7741 ccaatgccaa taaataattg attaatactt ttgtatattt ttggttatta tttaaactca
     7801 tatttttagt ggctttataa ccaattgtaa ctaatatagc agctacaatc attcctattg
     7861 ggtgttctac taaataaagt ctggaagtcg tttctttcat agcttcacct gaaaaattag
     7921 aaaatccaac tggagaaaca aaatataata ccaaacctaa tacaatttgt aggtgcgtaa
     7981 atatcaaacc tactaatgct gaaatttttg ccaatttatg atatggtttt ttcattaaga
     8041 aaaataccaa attaagtata actgcaacca ataaaactgt taggactagc atcccattaa
     8101 tgttgtgaat ttgtttaagt aatgcgtaca tgtttaaatt ttattttgaa tgtttaaatg
     8161 atgtgcaaat atatcttata aaaacaagaa aaaaccttaa tttgtattaa atcaatcata
     8221 aaatatagaa aaaaataaga ttgccatatt tttacaaaga gtaagtagaa tatgaaggaa
     8281 atgttttata tttataataa tggaaaataa aattgaatat caaaaactac aacatttgct
     8341 taacacagaa tctaaagatt ttgaattttt acaaaaactt tctgctgatg aattaagaac
     8401 tttacgtgta agaatgactg aagtaatgga tttagaccag ccagaaattt ggggacgagt
     8461 agcaaaagtt gctaagttta tgccaaattt tttgaatgca aaagtttctg aaactgtttt
     8521 aggtgcgttg attactgcca atatttcaac ttatgtccca ataaaagatg ctatttcaat
     8581 aatgaaaaat ttatctacac catttttagc agaagtggca gaatatatga ttcctgaaaa
     8641 atctgaagaa ttaattgaaa atatatctgt agatactttg aaaaaaataa cgacgcaatt
     8701 acttaaaaat aaaaaatatt atgtagcttc tggctttgtg aatgtattgt catttcctaa
     8761 aataaatgcc atatcagaag tcattactga tgaattagat ttaattctca tagctgaatt
     8821 tgtagaaaat aaagcccata ttgcaagaat tgtggagggt ttttctgata aaagaatatt
     8881 aaaaattatt cagactgcct ataaaaataa taaacaagaa gaaatcttga ctgtattcaa
     8941 gttacttagt ccaagagaac ttacaagagt tttatcgata gtaaagcaat tgtccgatac
     9001 tgaacaaaat actattttac aagatttcgt tactcgaata aaataattat ggaacaagaa
     9061 caaacacttt ttgaatctta caagaaaaga ccaaaactcg atgaacatta tgatgtgatt
     9121 tgtattggtt ctggcttagg ttctttatgt actgcatctt tattagcaag aaatggcaaa
     9181 aaagtactcg tttgtgaaaa acattataca cctggaggat acacccatgt ttttactaga
     9241 aacgattatg aatgggatgt tggtttgcat tacgttggcg atatggacag aaaaggttct
     9301 gctctaagaa taatttttga atatataaca gatagaaatc taaagtgggc cgatatgggc
     9361 gaagtttatg accgagtact cataaatggt aaaacttttg agtatctgaa aggtttgagc
     9421 aaatggaaag aaagaatgaa aatctacttc cccactgaac aagatgccat tgctattgat
     9481 aaatatgtag agattttatt tgctgtcaat aatgcgggca aaagctttta tatgcaaaaa
     9541 gcaatgccca aattagtaca atttttcaca aaaccatttc tcaataaatc ctatcaaaaa
     9601 tattatagtc aaactaccaa agaattatta gacagtcttt cagacaatga ggaactaaag
     9661 gctgtattgg ctgcacaatt tggagattat ggaatgccac caagtcaaag tagctttgta
     9721 attcatgcag ctgtggcttt acattatatg aatggtgggt tttacccagt tggtggctca
     9781 gaagaaatat tcaatacgat aatgccaagt attttaaata ctggtggcaa aacaattatc
     9841 agtgctgaag tcaaagaaat tttagttgaa aatggtaaag caattggcgt tttaatggca
     9901 gatggtaaaa agattacagc agatattatt gttagcggcg ttggtgctca tctaactttt
     9961 gaaaaactat taaatactga aaatcagaaa aagattaagt tttcaaatga cttaaaaaat
    10021 ctaccgccat cttatggaca tttcagttta tatattggat tgcaacatac tgcgactgaa
    10081 ttaaatttac caaaagccaa ttattggatt tatccaaacg gcaatgacca tgatgccgca
    10141 gtagaaaagt ttatgaatga ttataatgaa gaatttcctg ttgtttatat ttcatttcca
    10201 tcagccaaaa accctaaatt tgatgaaaaa tacccaggca gagcaacaat agaaattata
    10261 tctgttgcaa actacgattg gtttacagaa tgggaaaact ctaggtggaa aaagcgagga
    10321 gaaaactatg aaacatttaa ggaaaagatt gctcaacgtt tattagcaca tttatacaaa
    10381 gtagaaccac aagtagaagg aaaaattgac acttatgaac tatctacacc actttctact
    10441 aagaattttt gcagttatca aaaaggagaa atttatggac taacacatag tccacagcgt
    10501 tttgattcag aatatttaaa accacaaacg ccaattaaaa acttatactt aactggacaa
    10561 gatattgtaa ctgttggtat tgcaggtgct ttgatgagtg gagttttaac aacatcagca
    10621 attctaggaa aaaatataat gaatgatatt attcagtatg cgaaatcaac accacaatag
    10681 ctttatcctt gttttaagat aattctaaaa gttgttcctt tatcaattac tgaatctttt
    10741 acaaataact ttcctttgtg atatgtttga attattcttc tacttaaagc taaacctaaa
    10801 ccccagcctc tttttttagt ggaataacct gtgttaaaaa cagtttcaaa atttccagat
    10861 ggaatacctt tcccagaatc ttttacatca atataaacta aattcttatc ttcaaaaata
    10921 atcaattcta ttttaccttt tctctccatc gcatctaaag cattcttaat taaattttcc
    10981 aatacccatt caaataattg ttgattaata agcactttag cttctctatt ggtcgtatct
    11041 tttatttgaa aaacaattga ataggctgca cgttttttca tatactcaac aacattttct
    11101 acgataggca ataacaattc aactttcaat tctggtggcg aaccaatttt agaaaatcgt
    11161 ttggcaacta aatctaacct atctaaatct ttatccattt cttctaacat taccatactt
    11221 tcaacatcat catttaatct atctttcaaa atttctaccc aagcagaaat agaggttagc
    11281 ggagtgccta attgatgtgc tgtttctttt gctaaaccta cccaaacctg attttgttcg
    11341 gctttccgaa ttgaggcaaa tgctataaaa ctaatcgaga aaaataatat tactaaaaaa
    11401 agttgatagt atggatattt tttaattttc tgtaaaagaa tggaatcttt gtaatataaa
    11461 aaatgagttt ggttttcatc aatatgaata acaatctttt tattcttgag tttatgaatt
    11521 tgctgtttta aataactagc atctgcttca actttagtgg aatcgaaatt tttataagct
    11581 aaaatattat tttgactatc agaccaaata attggaatat tatcatttat ttttataatt
    11641 tccaaagcat catctaagtt accaccatct ccactaatta aaatatgata agcatttgcc
    11701 aaatcatttg cttgtttgat ttcttgcttt ttaatgaccg atgctaattg attcgtgtac
    11761 caaattgaaa aaccaactat tataattcct aataagaata atagatattt gttcaaaatc
    11821 agcaacttaa ttttttcttt cataaaatct gaaaataaag ataatttttt ttgtaaaaaa
    11881 acttgcttaa ttaaaaataa atatcgtata tttgcacacg cttttgagga ttacatcaaa
    11941 tagtatctaa aaaagtaatt agttcttcaa taaatagaaa atagacactg agtaatcagt
    12001 agatataaaa gggcgcatag ctcagctggt tcagagcacc tgccttacaa gcagggggtc
    12061 cgcggttcga acccgtgtgc gcccacaccc tttataaagc acttacaaat acagtaggtg
    12121 cttttttatt ttaagactaa atctattcta tttacaatta gcttttataa tttctacaaa
    12181 cttttttcta ggtatatgaa ttgcacccaa actactcaaa tgtggtgtat atacttgaca
    12241 atctattatg acagcacctt cagattttag ctgatttaca taagtaataa atccatattt
    12301 actagcatta tccatcttat gaaacatcga ttctccaaaa aaacactttc ccatctttat
    12361 gccgtacaaa ccaccaacta attcatctcc attccaaact tctaccgaat gagcatagcc
    12421 ttttttgtgt aaattaatat atgcatcaat catttcatca gttatccaag taccatcttg
    12481 aaaatttctt tttgcattag cacattcatc aataacttct ttgaaatttt ggttcgttgt
    12541 aaccttaaat ttatttttat tcaataacaa tcgcatggat ttagaaacat ataaatcctc
    12601 agaaaaaagc acaaaacgag gatctggcga ccaccaagca ataggctcat ctttagaaaa
    12661 ccacggaaaa attccagagc tataagccaa taataagcgt tcttctgata aatcgccacc
    12721 aattgccaag attccttcat ctaaagcatt ttcaacaggt ggaaaccaaa tagatttatc
    12781 taaaactact aagcccaaaa tactatacag attctaaagt tgcggtaatt cctctatcca
    12841 atagagcatc tttcattgga agtaattcct cttcagtgcc atgttttact gcatatttac
    12901 ctttaaaatg aataaacaat gcacattgtt ctgcttgttc gtaagtatgc tgacaaattg
    12961 ccactaaaga ttcaataacc cactcaaaag tattaacatc atcattatgc acaattattt
    13021 gccaaccata atctgtttcc tccatttcca gcacatcagt atctggattt tcctttgttt
    13081 gagtatgaaa aaaaaattga ttaaaatcca tagtaaatac aaaaataaca aaatgtattt
    13141 gtaaaatcaa ttataaaata ttatctttgc acacctttag aaaaaatccg gcggggtagc
    13201 tcagatggtt agagcgcagg attcataacc ctgaggtcac gggttcaatt cccgtctccg
    13261 ctacaaagtt tttagcctaa aattttaatt ttaggctttt ttattttata actttatgtc
    13321 gttattaact tcttttactc aaaatataaa ttcctaataa aaccaaaata gttcctagaa
    13381 tatgcatcca tgtcatgctt tcgcccaaaa tataataagc caaaaacata gtagaaaccg
    13441 gtccaatact tgcaataata gacatatttg atgaaccaat caaactaata ccatacgtca
    13501 ttaagaaaga aggtaaaacc gttgctccaa ttgcaataat tccacagata atatatatat
    13561 tcattgaaaa actcaaaaga ttaaaaccat tgactaaaaa attatgtatc aatacagcaa
    13621 tacaagaaac catcattgat aaacatgtaa aacgaagtgt accaatgttt ttaataatat
    13681 tatcactacg aactaaataa aaggcataag ttaaagcact caataaaaca aatacaacac
    13741 caataaaacc ttttttaaaa tccattgaat cgatatctgc ataaaatgct aatacaattc
    13801 ccacataaca aattaagaca gaaattaatt gaacaagtga aacttttctt ctataaaaga
    13861 taaatcctaa aagcaaaaca aaagttggat aaataaatat cgtaatcctt tccaaatttg
    13921 cagaaacata ttctaagcct ttgaaatcaa ataatgccgc taaataataa ccaattaaac
    13981 ccaacaacac aatatcccaa cgaatggaag gctttatagc aataggcttt cttttagttt
    14041 ctctaaaata gatgaataaa taaaatggca aggaaaatag cattcttaaa ttcaaaacat
    14101 ctaatgaact tacgccatat cgatatgcca attttgctaa tacaccttta gatgaaaagc
    14161 aaatagctcc aataattaca aaaaaaatac ctttttgttt ttgactcgtt aaatgaaata
    14221 cttgtttgtc cattggacaa ttaaatgtaa aaaaaatatt ttaatctaaa aacggtaata
    14281 cttttggccc ttgtgcatta actttactga tataaacttc tgcaccaata cctaaattat
    14341 tcattacctg cttcatcgca tcgcccactt tatttgcaat ttcttgtcct ttgcacaatg
    14401 caaaaacact aggtccgctc ccactaatac tactacccaa tgctccactt tctaaagctg
    14461 catttttcat atcataaaaa ccaggaatta atttacttcg atgcggctct gcaatataat
    14521 caattaagct tctaccaatt aaatcgtaat cgccacgtaa taagcctatc atcaaaccac
    14581 caacatttgc actttgtcgg agtgcatgcg tcaagggaat ttcttttggc aaaatttctc
    14641 ttgcttcttt tgtcagaaca attacatctg gattaattac aacacaccac aactcttctg
    14701 gtactggaat tttaataata tctaatggtt catagcttct tactaaaata aagccaccaa
    14761 aaagacttgc cgctacatta tcagcaatgg ctgcatcaca agctgctttt tcagcattga
    14821 ttaaaaaagg tagcatctca tttttagtca attgctcttt acatagcata ttgattgcat
    14881 aaacaccagc tactgcactt gctgcactac ttcctaaacc actaccactt ggcatcattt
    14941 ttttaagcca aattttacaa ccaaaatctg catttatata attcaaataa tcttgaatcg
    15001 ctactgttgc tgaatttttt ttagcatctc taggtaaaac gccaccatcg ccttcaatat
    15061 caacaattac aacccctttt tcggcagtga tttccataat caattcgtcg ccaggttttt
    15121 caatagcaaa acctaaaata tcaaatccgc aagctacatt ggcaactgtt gcaggtgcat
    15181 aaactctaat tctatctcca atcttcattt cttagttcta aaatattaat tggctaaata
    15241 atttcctaaa ctgataattt cagagaatac accagctgct gtaacttcag ctccagctcc
    15301 tggtcctttt atcaccaatg gattattttt atagcgttct gttgtaaaaa caatcatatt
    15361 atcactacca ctcaaattat agaatggatg ttgagcatct acttctttca aagcaatttc
    15421 tactttatta ttttcaataa ccgccatata tcttaatgca ttgcctttct tctctgcttt
    15481 tttctttaat ttatcataaa ctgcatctaa atctgtaagt ttctgtaaaa atgcttttac
    15541 atcaatatct ctcaattctt tagatactaa gttttcaact ttaatttgct ccaattctaa
    15601 ttttgcacca cattctctag ctaatatcaa agcttttctt gcaacatcta atccgcttaa
    15661 atcatctcgt ggatctggtt ctgtaaatcc ttttgttttt gcttctttca ctacatcaga
    15721 gaataaaata tcgcctttat aggaattaaa aatgtaagaa agcgtacccg acaacacggc
    15781 ttctattttt ataattctat cgccagaatt taataatgca tgcaaagtat taattactgg
    15841 caatcctgca cctacattag tttcatacat aaaacgagag ttagctcttt ttgctgtttc
    15901 tctcagagat aagtatttcg gataggctaa tgtattggca attttatttg gtgtaacaat
    15961 cgaaatactt ttcttcaaaa tgcgttcata atgttccact actgatttat ttgcagaaca
    16021 atcaataatc acagtatttg caaaattcaa atctatagct ctattgataa aattattaat
    16081 atttgcatct tccctttcaa ctttcaattt tgattctaag ttttcaatat caataccatt
    16141 gctatctatc aacatatttt tagaattgat aacaccaatc aaattgattt ctaaattttt
    16201 ttcattttct aagtaacttt tctgatggct aatctgcttc aataaagttc cgccaattaa
    16261 cccagtataa cctactaaaa atacattaat agaaagttta tcttgttcaa agaagatttc
    16321 atgcaaagca tttaaggctt ttgcttcatt atacttatca acgattgcag tgatatttac
    16381 ttcagaagaa ccttgagcaa ttgcttttac attgatacca tttttaccca aagctcggaa
    16441 catttttgca gaaacccctg gcacattgtt catattctca ccaattacag cgatgatact
    16501 attttctaat tcaatgacta atggttgaat aaatttattc tgcatttcat atctaaactc
    16561 atcttcaaga gcttttctac attttttcac atcattaggc aataccccaa aacaaataga
    16621 atgttcagag gaaccttgtg ttaaaaacat aatattgata ccagcttttg aaaaagcacc
    16681 aaacaaacga gatgcaattc caacaacacc caccattcca gtgccttcca ttcttaaaag
    16741 tgcaatatca gaaactgagg aaatgccctt aatcatattc actgacttgc cagaattttt
    16801 ggaaataaaa gttccaggat gttcaggatt gaacgtattt tttatataaa taggaatacc
    16861 tttaacataa gctggttgta tggtcggtgg gtaaattact tttgcaccaa aatgagataa
    16921 ctccattgct tctttataac ttaaagcagg cactgtcatt gcttggtcca ctcttcttgg
    16981 atcagcagtt aaaacaccat caacatcaga ccaaatttca atagcagaaa cttttaaagc
    17041 agcaccaaaa atggcagcag tatagtcaga accaccacga cctaaagtgg taatgtcatt
    17101 aaattcatct gtaccaataa atccagtaat tacaggaata ccgtgcaaat gctcaaaata
    17161 ttcaagtata tttttatttg ttctgtcaaa atttactttt gctgcaacat aattactatc
    17221 tgtttttatc aattgtgtgg catttacata aacacctttc aagtttactt ctgtcaaagc
    17281 accagcaata ataaaacaag ataatctttc tccaaagctc attaatctgg ctaatgtttt
    17341 atcagataat tcagccaaca aacttacacc atgcagaata tcatcaactc tttgcaaacc
    17401 ttcttctaat tcttctaata atgcaggttt aattgatttt ttagatacta atgcgtgaat
    17461 agctgcaaga tgtctttctt taatattttc taaaactgct ttgtaattat catctctttt
    17521 ctcagctaat tttccagcat ttattaattg atctgtgatt tttttaaatg ccgagcatac
    17581 taaaatcaca tcgttacctt gttttttctc gttttttaca atatcaataa tccttttgat
    17641 attttctacc gaacccactg atgacccacc aaatttaaga atcttcataa tttattttta
    17701 aaacaatata tatttgtact ttttcttttt tgaaacaaac aaaggtaata aatacaaaca
    17761 taattttgta tgtttggcaa cacaatgaaa ttaatttaaa attaaatgtt aaagttatta
    17821 catggcattt ataccttatg gctaagtctt acttttttta tcatgacgcc actgatgctt
    17881 ttttgttttt tgatgataaa attatttatt ccatatccaa aacaaatcca atttgtatat
    17941 tatgtaaata gatttttctt ctttttttgg tctgccatca atggtattcg ctataaaatt
    18001 acaggattag aaaatatcaa taaagaccaa acttatattt gcgttttaaa tcataccaat
    18061 tcaggcgata tggttgctgg tgcttatgga tgcagagtaa atgcgaagcc attaataaaa
    18121 agagaattaa ctttaattcc atttttagga caaatgcttt ccattgcttg tattgccgtg
    18181 gatagaagta gcaaagaagc tcgaaaaaaa agtaaggaaa atatgttgaa tgatttaaaa
    18241 caaaaaattt ctgtacttat ttttgctgaa ggcacacgaa acaggactgg caaaccatta
    18301 aaagattttt ttgatggtgc ttttgatttg gcaattgatg cacaagtacc aattattcca
    18361 attgttttga ctaatataaa gaaaattaac ccaaaacatg attggttttt tagacccaca
    18421 actattgaag taagacattt agctccaata tttccagaag gcaaaactga agaatgtatt
    18481 caaaaattaa aagctaagac ttttaaaaat atgtggaatt gtttggtaga atttgatgat
    18541 gaatttaaaa aatatgacaa gctttaaaat atgtacggca tttgtaaatt aacagcaatt
    18601 ccaatgcgtc aagagccttc tgacaagagt gaaatgatta atcaagtcat ttatggcgaa
    18661 acttttgata ttctgcaaaa agatgaaaaa tggtcaaaaa ttaaattgca tcatgacgaa
    18721 tatgaaggtt ggatagataa taaacaatat gaaaaatcga caaaaaaggc ttctccaaaa
    18781 aacattaaca acaaaaaata tatttcaaaa gaacacaaaa ttatcccact cggtgctttt
    18841 ataaattttg aaattaatac ttcaaaaaaa tcaataattc aaactgccaa aagtttatta
    18901 aatacaccat atttatgggg tggtcgcact tgttttggga ttgattgttc tggctttaca
    18961 caaattattt ttagagttca tcatatttta attccaagag atgcatacca acaagctgaa
    19021 ataggcgaat ttgtacaact taaagacact caaaataatg atttagcatt tttttccaat
    19081 ccaagtggaa gaatcattca tgttggcatt attattagaa ataaaaaagg cataagcatt
    19141 atacatgcat ctggaaaagt aagaattgac caattagatg aaaaaggaat ttttaatcaa
    19201 gaaattaatg cttattcgca ttacttacat agtataaaac gtattactaa gtaaaaattt
    19261 attttaaaag tactaatgta acatctgtta ctaaaactag attttattca acatatcttt
    19321 gcatcattaa atataaaaag catgaaaata gtgattattg gaggtgttgc tggtggtgca
    19381 actgcagcaa ctagattaag acgtttatca gaagaaaatg aagttattat ttttgaaaaa
    19441 ggtcaatatg taagttttgc aaattgcggt ttaccttatc atattagtgg aactattgaa
    19501 gaaagaaatg cccttttatt acaaacacct gaaagtttaa aagaaagata taatttagat
    19561 acaagagtat tttctgaagt taccaatatc aatatagaag aaaaaaaagt tatcgtaaag
    19621 aatttgcaaa ctgatgaaat ttatgaagaa acttacgata aactattact ttctcctgga
    19681 gcagaaccta ttcgtccacc atttgaaggt ataaactctg acaagattta tacattacgt
    19741 aatattcctg acatggatag gattgtacaa aaaaccaaag acgctcagaa ttttgttgtt
    19801 gtaggtggtg gatttattgg cttagaagta gctgaaaact taattgaggc aggcaagaat
    19861 gtaaaattaa tagaattagg aaatcaagtg atggctcctg tggattatga tattgcaagt
    19921 tatatacatc ataaagccaa agaaaaaggc ttagagttat tattaaatgt tggtgttcaa
    19981 aaatttgaag accacaaaga ttccattaca gtatttttaa ataacgatac aagtatcgaa
    20041 acagatgccg taatcttagc cataggtgtt aaacctgaaa ctcaattagc aaaaaatgtt
    20101 ggtttgatga ttggtgaaac tggtggaata ttggttaatg gatttatgca aacttctaat
    20161 cctgatattt atgctgttgg tgatgctgtt gaagttgaac attttataca tcaacgaaaa
    20221 gtattgattc cattagcttg gcctgcaaac agacaaggtc gtttggttgc agacaatatt
    20281 acttttggaa ataaaaataa atattctgga agcttaggtt cgtctatttt aaagttcttt
    20341 gaactttcta ttgcttcaac aggattaaat gaaaaattct taaaaagaaa taatattcct
    20401 cataaaacag ctatcgttac acgtgggcat catgctggat attatcctgg tgcaaaaaat
    20461 attactttaa aattgatttt taacgatgaa ggtaaaatct atggagctca agcggttggc
    20521 gaaaaaggta tagataaaag gatagatgta attgccactg caataaaagg caatttaacc
    20581 gtttttgatt tacctgaaat tgaaattact tatgctccgc ctttcaattc tgctaaagac
    20641 cccgtaaaca ttgtaggtta cactgctgaa aatatgttga atggaaattt ggaaatgata
    20701 aattatgatg atttattcca atatcaggaa gacaaaaatg ctatactttt agacgtaaga
    20761 acacctactg aatttgcaag aggaagtatt aaaggctcca tcaatttgaa tgtagatgac
    20821 ttgagagaga atcttacaca attagacaaa aatcgtaact atgtaatcta ttgtcaagtt
    20881 ggtttgcgtg gttatctagc ttacaaaatc ttaaaaaata atggttttaa ggtcgtaaat
    20941 ctaaatggtg gttatggatt atggtcgatg atata
//
LOCUS       DAOQCW010000032        20808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_922_length_20808_cov_5.498289, whole genome shotgun sequence.
ACCESSION   DAOQCW010000032 DAOQCW010000000
VERSION     DAOQCW010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 20808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20808
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_922_length_20808_cov_5.498289"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="PLS16_04370"
     CDS             complement(<1..727)
                     /locus_tag="PLS16_04370"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ60348.1"
                     /translation="MLESILIKNYAIIESIELDFHNGFNIITGETGAGKSILLGALGL
                     ILGKRADTQVLRNKEEKCIIEAKFDIQQLQIESFFEENELEFERQCIIRREIQPNGKS
                     RAFINDTPVNLSQLQELSNALVDIHAQGETQKLLDEYFFCSILDKLAGQEADTENYYH
                     QFLEYKQLNKKLHQIQSEREQLQLEYDFNLFIYKEFEQIDLAEDVYNNLEQEIDIQQN
                     AELIIQNLRSIEKLLDENEYSVIP"
     gene            complement(751..2700)
                     /gene="gyrB"
                     /locus_tag="PLS16_04375"
     CDS             complement(751..2700)
                     /gene="gyrB"
                     /locus_tag="PLS16_04375"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002506219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNJ60349.1"
                     /translation="MSDTNKVNYGADNITVLEGLEAVRKRPAMYIGDINNRGLHHLVY
                     EVVDNSIDEALAGHCKNIYVTIHENNSISVEDDGRGIPVDIHPKEKRSALEVVLTVLH
                     AGGKFDKDSYKVSGGLHGVGVSCVNALSSYLKVNVYREGKVYEQEYSIGIPKYAVKEV
                     GTTDKRGTRVHFLPDASIFTVTEYNYDTLAARMRELAYLNRGISITIADERQKKDDGT
                     IFTENFYSEGGLKEFVQFLDKTRQAIIPEPVYMEGNKDSVAVEVAFQYNDSYSENIHS
                     YVNNINTIEGGTHVVGFRRALTRVFKNWGTKNDLFKNVKVEISGEDFREGITCIVSVK
                     VMEPLFEGQTKTKLGNSEVDGIVAGVVGEMLEYYLEENPKEAKTIVNKVIIAATARHA
                     ARKARELVQRKNVLSGSGLPGKLADCAEKDASICEIYLVEGDSAGGTAKQGRNRAFQA
                     ILPLRGKILNVEKAMEHKIYENEEIKNIFTALGVFKGVEGDEKALNTSKLRYHKIIIM
                     CDADVDGSHITTLILTFFFRYMKELVESGYIYIANPPLYQVKKGNKQAYAWNETQRLG
                     YIKEFAGEGKEDSVHVQRYKGLGEMNAEQLWSTTMDPETRTLKQVTIENAAEADRVFS
                     MLMGDEVPPRREFIETHAKYAKIDV"
     gene            2852..3262
                     /locus_tag="PLS16_04380"
     CDS             2852..3262
                     /locus_tag="PLS16_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60350.1"
                     /translation="MSITEKLYIVPNEVDIPQNSNGLALIIKKYEDEKVTQNSLEMLQ
                     KLIEGGLSLKFSETLVLQITDKEQFKLPNSINKVFLFGVRPQQIGLNINSLRNKIMRV
                     NHQSIVWTDETALMINDGAKKKAFWIEISKIYKV"
     gene            3259..3855
                     /gene="rdgB"
                     /locus_tag="PLS16_04385"
     CDS             3259..3855
                     /gene="rdgB"
                     /locus_tag="PLS16_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNJ60351.1"
                     /translation="MIALVFATNNQHKIDEINVALPQNIQIKSLASIQCFQELPETHF
                     TIEENAEEKATYVVENYKVNCFAEDTGLLIDELNGEPGVFSARYAGEQRNNEDNINLV
                     LKKLEGKTNRKAHFKTVIALHLDGKLYLFEGILNGIIIHERRGSNGFGYDSIFQIEDG
                     RTLAELTMEEKSKISHRAIATQKLVSFLNLYSITNKSI"
     gene            3892..4428
                     /locus_tag="PLS16_04390"
     CDS             3892..4428
                     /locus_tag="PLS16_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNJ60352.1"
                     /translation="MPKQNYCSNCGNAVVYQHAEGRERCVCNNCNIIHYTNPKIIVGA
                     LTYWEDKILLCKRAIEPRKGFWNIPAGFMEDDEKTEDGAAREVWEEAEAKINIIQPYL
                     IYNLPQANQVYIHFLAELKDGVYGNGVESLETRLFLEDEIPWNEIAFYSSTFALKRFL
                     EERKTGNFKTHLASFPDK"
     gene            complement(4429..5760)
                     /gene="nhaA"
                     /locus_tag="PLS16_04395"
     CDS             complement(4429..5760)
                     /gene="nhaA"
                     /locus_tag="PLS16_04395"
                     /inference="COORDINATES: protein motif:HMM:NF018648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HNJ60353.1"
                     /translation="MDKLKHQPVDKWIIEPINKFINNSIASGVVLFLAAIIALVLSNS
                     PFSEQFHNIWEHQVVFAFDKYILSKNLSHWINDGLMSIFFFVVGLELKRELVAGELAR
                     PKQALLPILAAIGGMVVPAIIYHAFNHDASTERANGWGIPMATDIAFALGVLYLLGDR
                     VSNGLKVFLTALAIVDDLGAVLVIAFFYTSDISFVSLGFAALFLSIMIIANLMGVRSA
                     IFYGIIGVFGLWTAFLMSGIHATIAAVLAAFTIPATVKIEEDGYIEKINHLINDFKNA
                     SRTHFKIVTDEQQKILSEIRLYTDAALTPLQKLIQSLHPLVAFIIMPIFALANAGVAF
                     DESLSSAFTSKVTLGVFLGLLVGKLVGVFGTVYLLTKFKIAVLPSNSNYKQMVGVSFL
                     AGIGFTMSLFVTSLAFNEVHYTTEAKIGIFSASILSGIIGYFILRNTKTTA"
     gene            5909..7702
                     /gene="lepA"
                     /locus_tag="PLS16_04400"
     CDS             5909..7702
                     /gene="lepA"
                     /locus_tag="PLS16_04400"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNJ60354.1"
                     /translation="MQNIRNFCIIAHIDHGKSTLADRLLEFTKTVSERNMQAQALDDM
                     DLERERGITIKSHAVQMDYTHLDGTKYVFNLIDTPGHVDFSYEVSRSLAACEGALLLV
                     DATQGIQAQTISNLYLAIENGLEIIPVINKIDMDGAMIEEVTDQIVDLIGCEAEDIIH
                     ASGKTGIGIQDIMTAIINRIPAPKGDENAPLQALIFDSVFNSFRGVIAYFRVKNGSIK
                     KGDKVKFVNTGRAYEADEVGILRMSLEPRNEVKAGDVGYIITGIKVSKEIKVGDTITQ
                     LNNPCKEAIKGFEDVKPMVFAGIYPIDNDDYEELRESLEKLQLNDASLVFEPETSLAL
                     GFGFRCGFLGLLHLEIVQERLEREYDMTIITTVPNVTYYAYTKDNNKLIVNNPSDLPE
                     PNLLDYVEEPYIKANIITKPEYIGTIMKLCMDKRGILKNQVYLTETRIELQFEMPLAE
                     IVFDFYDQLKSISKGYASFDYHMIDFRESDLVKLDIKLNGENVDAFSALIHRTKAYHF
                     GSRLCEKLKELIPRQQFLIAIQAAIGAKIIARETISAMRKDVTAKCYGGDISRKRKLL
                     EKQKKGKKKMRQIGKVEVPQQAFMAVLKLND"
     gene            7832..7918
                     /locus_tag="PLS16_04405"
     tRNA            7832..7918
                     /locus_tag="PLS16_04405"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7866..7868,aa:Ser,seq:gct)
     gene            7939..8013
                     /locus_tag="PLS16_04410"
     tRNA            7939..8013
                     /locus_tag="PLS16_04410"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7974..7976,aa:Pro,seq:tgg)
     gene            8158..9828
                     /locus_tag="PLS16_04415"
     CDS             8158..9828
                     /locus_tag="PLS16_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789599.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HNJ60355.1"
                     /translation="MQTLATQDYIVFLLYFILIVGYGWWIYKRKQKEQTSTKDYFLAE
                     GSLTWWAIGASLIASNISAEQFIGMSGSGFKMGLAIATYEWMAAFTLILVAVFFIPVY
                     AKNKIFTMPQFLNQRYNGKVAMIMAVFWLLLYVLVNLTSILYLGALAISSISGLNLTL
                     CMIFLAIFAVMITLGGMKVIGFTDVIQVFFLILGGLVTTYLALNLVAEKFGMTGIVNG
                     FSLMREHANDHFHMIFDKSNPNFIDLPGLSVLIGGMLIINLNYWGCNQYITQRALGAD
                     VKTARNGILFAAFLKLLMPVIVVLPGIAAFVLYQKGGFQAEMLQDGSVNPDKAYPTLL
                     NLLPTGLKGLSFAALTAAIVASLAGKANSIATIFSLDVYAKAIDKYADEKKLVFIGKL
                     TIIISMIIAVIVAPFLGIDKKGGFQYIQEYTGFVSPGVFAMFILGFFWKKTTPNAALF
                     AMIGGFLMSIIFKFMPEFVDLSFLHSAGFSVPNSVGIYEIPFVDRMGFVFAICVIGMY
                     FISIYETNKGIQTQGLEVDASMFKTSTGFMIGSIIIIGILAALYTVFW"
     gene            complement(9870..11708)
                     /locus_tag="PLS16_04420"
     CDS             complement(9870..11708)
                     /locus_tag="PLS16_04420"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNJ60356.1"
                     /translation="MVLIFFNGSKTINNNATFRDYILQEDNNLTYNEQNNTTNKDFNN
                     RLNLKIEYKIDSSNILTFTPNFSIQNNNTNSILNANNLRNDSITESSTQNNQFAQQLG
                     INFSNNILYQHKFLKKGRTISANFNTIYGIRNTDRDLYSLNSILFNEDTINQKSILNS
                     NNYTIGGNIVYTEPIKKNGQISINLTPSFTNNYSIKNTDNYNLLTQDYTSRDSILSNE
                     FNNKYITNKLGLAYRFNNQKINWMIALNGQSALLKSEQYTPQNSNINKHFLSLLPMSF
                     LNIRFSKTQNLNIGFRTSTSAPSITNLQNVIDNSNPLILTTGNPDLKQSFTSSLNIRY
                     SRTKPENASSIFLFANTSNSMNRVANSTTIFTNDTIIDGIQFTPGMQFIKPINLSGYW
                     NARALINYGFPFKAIKSNVNINGGFVFQRTPSLINTEKNYSNTYNFNAGISLSSNIST
                     KIDFNLSYFASYNLIRNSLQQRNNNNYFNHTASARLNYQFWKGFVFSSQVSNTLNAGG
                     SDAYNTNYWLWNASLAYKLLKNQSLEIKLSVNDILNQNRSITRNVTDAYTEDVHTTAL
                     QRYFLGTITYTLKKVGIGNDDNKLKDFMLPPSGAMPPHGAMPPPNH"
     gene            complement(11689..12774)
                     /locus_tag="PLS16_04425"
     CDS             complement(11689..12774)
                     /locus_tag="PLS16_04425"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNJ60357.1"
                     /translation="MRFYILASILYFFQLQLFAQQSHLNDSTIQETANRFSIKGTIID
                     AKTKEALIGVDVFLKSITDSTITKDAITDFDGNFILENIEKGNYKLSLKYISYKTNEL
                     NLQINHDTDLKSLELTEDSKLLKEVVIESTQIRVQQLGDTSQYNANAFKVNQDATTED
                     LLTKMPGMTSENGTIKVNGEEVKKVLVDGKEFFGSDTKAAIQNLPADIVDKIQVFDKL
                     SDQSAFTGFDDGEGVKTINIVTKGGISSSQFGKIYAGYGGPNNRYNTGLNYNTFQGNR
                     RISILGMSNNINQQNFNIQDLMGSLSTGNSGFRPGGGGPPRGGNPINNFLVGQRGGIS
                     TTTAFGLNYSDIIGKNKKVNISGSYFF"
     gene            12912..13916
                     /locus_tag="PLS16_04430"
     CDS             12912..13916
                     /locus_tag="PLS16_04430"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HNJ60358.1"
                     /translation="MRNWITSEKLVQQNQRNYGRFERTPIANPLDEFSGIKRLFNNIR
                     LKEWVGFALFHPDIYGAMIIQNAKIIKSSDIHIFENSNYTNFQSISISSKLKISEDLL
                     HSTLHFNKSAYSIKFDFTDSRVAISIHIKATKNNQTIKGEIVLDVSRKSKPLVVSALL
                     PPLGTMYTNKIIYPASGYLQVGDNRYDFLAERDILILDEHKSRLPYKTEWTWGTFAFP
                     IENSFVGANFAKRPQYSDQEEESCLWTSSGVEALKDIVFEPQDNAQNAPWHIYSKDGR
                     LDVVFTPKGQKDVRENAVIFSINYFQMFGTYSGKIVGKNKVWNFENIHGYCEQMKMKA
                     "
     gene            complement(13934..15019)
                     /locus_tag="PLS16_04435"
     CDS             complement(13934..15019)
                     /locus_tag="PLS16_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60359.1"
                     /translation="MKNLMILFAVFILFFSCTKEEKSQKTKEPDPKKDQQSVNGKIDF
                     VGSIALSEGPHQLLIDDNNLYAKRDNQIFLFSLNNPQVPQQQNKYTQTYNKNFGKMTF
                     HNDKLYVCNIDEKAVYVFDEDLNFEVKYTFSSSNLKPNILLIDDDNNFWVGGSNGANG
                     IVAKCTLSANILNIDKEWQHTSAQSNFEWLEEEDGYILATNALGEFITFKQSNFPSTA
                     TTITYTNESGHEKWGKSFVTVNDYAYWANWGAGIATVDISNPFLISVTKIISNSSFKN
                     QFASAEGTNAYDVAYNEKHDYICVANGWSGVLLVKPNQSDKVDDFLDPQYFQNMSIAT
                     KEDYIYTGNISGGMSGDLKGIKIFKLK"
     gene            complement(15332..16270)
                     /locus_tag="PLS16_04440"
     CDS             complement(15332..16270)
                     /locus_tag="PLS16_04440"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNJ60360.1"
                     /translation="MSKRGYISRYLLILKKLKAKPYSTYVELQTYIENQFDFLQMQDD
                     TLNIGFSKRTLQRDLKEIRNVFGIDIEYSKANKGYFISQSETENMNFQRMIEAFDMFN
                     SLNLAQDLTPFIHLEKRRPQGTENLYGLLHAIKNQFQIKFVYQKYWEEVLKERLVEPY
                     ALKEFKNRWYVLAKDLGDNKIKSFALDRLTDLEILKKKFNFPTNFDVNEHYRYCFGII
                     SPNGHQPEDVILSFNTFQGKYIKSLPLHDSQQILIDNKDELRIQLKLFITHDFFMELL
                     SFGENLKVLKPQSLIDDLKSTVRNVQRKYEKQKLSK"
     gene            complement(16408..16773)
                     /locus_tag="PLS16_04445"
     CDS             complement(16408..16773)
                     /locus_tag="PLS16_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60361.1"
                     /translation="MNRIQEELKNVISNKYAFDDVTNMKLLEVLFLANQLLLKHNLRN
                     TKIEFRIHHSALGKCVNSGEIISIQINHAINNDMDEIRNTILHEIAHALVGNEYGHNI
                     VWQEKAKELGVIGTERYRK"
     gene            complement(16791..18362)
                     /locus_tag="PLS16_04450"
     CDS             complement(16791..18362)
                     /locus_tag="PLS16_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ60362.1"
                     /translation="MNTIIIGKEILERYFEENSFEENDVHSLNQLETSEDENIELDLE
                     KISKNLLEYILTNDTQINLQTKAEITLNYLKNDVVQNSNTESNIWDKKIKSLSDIMEV
                     YETLKKIKYNVDCLIFRRKNPAKLVAELLNATRHSPKRILFRLDFKIYKDTHSLNYEL
                     NNDNIISLRKEMSTLSFRDLMKIFNVFEQQENLSKYENRIVETKKTQKQDSKQILCKG
                     LGLSSHFELTNMGSFNKQSSCIVESELEIQVNNRYTNDSFKSHIELPYVRVFSLLYKE
                     YVYVHIDDISDYEYDEQAFQKLFLPKNLKNILEKVFSYSVENLTTDIINNKHGGLIIM
                     AEGNSGTGKTSTAEVYSELNKKPLYIVQVDEIGISPDKIEQNLNHIFRRVEKWNAIIL
                     FDEVDVFLSKRGDNIEKSAIVGIFLRLMDYFRGMMFFTTNRSEVIDDAVLSRITLNIK
                     YPDLTDDVRKLIWKSKLESAGLYIDSMDELIKINLNGRQIRNMVRLGKIIFDDKINEQ
                     KFIDLIHNSVPKSEK"
     gene            complement(18520..19092)
                     /locus_tag="PLS16_04455"
     CDS             complement(18520..19092)
                     /locus_tag="PLS16_04455"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNJ60363.1"
                     /translation="MHTIKIKNQNILLLSDTHGKHRLIEIPKNIQIVIHCGDICNDGD
                     IDEILDFFDWYAQLEIPHKLFIHGNHDLPFELEPEWNKRLIPKGIIWLNDESIVISDI
                     NIMGISAFPFQNGIVTKKRIDIIASHYPPQGILDNGFGSKEISNIVFQLVPKYHVFGH
                     NHSNYGKEKHKKIQYINASIYHQLCQKESQ"
     gene            complement(19079..>20808)
                     /locus_tag="PLS16_04460"
     CDS             complement(19079..>20808)
                     /locus_tag="PLS16_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ60364.1"
                     /translation="PRHTLSYLPSTFVKKDRFMESITKKQILESIGKVYEQSEKCKLG
                     NSFFESIKPELYTLSKYFKTSEHQTFFIAMVFALNYKGDTVDLNDLIKYFDCNPMKIL
                     EYSDDFDYLHKVGIFEKKKSRHRLKLAGANDQFAIHEKITEAILQNKPLPKINENKIK
                     SIIDLLEELYKLGDQRDDDKISTLELFLEVEKLILENMHFSLIEKVKQFKFGTKDTYL
                     LLYIIWKTVSGKETIDIGKALESIYDSPSERVNEIQKLLSGNHILTNNNLVEIIEAQF
                     FNDTEMKLTDTSLQLLNDCGIKLFLNKKKKDNIIVPSAVVYRELIFDNEEMSQLETLQ
                     NLLQEDKYHETQTRLTNKGLPKGIAILLHGVPGTGKTEIVKQLAKTTNREIMKVEISQ
                     SKSMWFGESEKIIKRIFTDYRAYAKESKLTPILLFNEADAIISKRKENSNSGVAQTEN
                     AIQNIILEELENFEGILMATTNLANNLDTAFERRFLFKIPFKKPTKEVRAKIWKSKLP
                     HLEAECCQILAEKFDFSGGQIDNIIRKSEIQEIVHGSVTNFEQIQSFCKEEFIITKTT
                     KIGFSYAHD"
BASE COUNT         6799 a         3180 c         3241 g         7588 t
ORIGIN      
        1 gaggaatgac agaatattcg ttttcatcta ataatttttc aatacttctt aaattttgga
       61 taatcaattc cgcattttgt tgtatgtcaa tttcttgctc taaattattg taaacatctt
      121 cagctaaatc tatttgctca aactctttgt aaataaacaa attgaaatca tattcaagtt
      181 gtaattgttc tctttccgac tgaatttgat ggagcttttt gtttagttgt ttgtattcta
      241 aaaattgatg gtaataattt tcagtatcag cttcttgtcc agctaatttg tctaatattg
      301 aacagaaaaa atattcatct aacaattttt gagtttctcc ttgtgcatgt atgtccacca
      361 aagcattact caattcttgt agttggctta aatttactgg agtgtcattg ataaatgccc
      421 gactttttcc attaggttga atttctcttc tgataataca ttgccgctca aattcgagtt
      481 cattttcttc aaaaaaagat tcaatttgaa gttgttgtat atcaaatttt gcttcaataa
      541 tacatttctc ttctttattg cgtaatactt gtgtgtcggc tctcttacct aaaattaaac
      601 ccaaagcacc taataaaatg gatttcccag cacctgtttc gccagtaata atattaaaac
      661 cattatgaaa atccaattcg atagattcga taatggcata atttttaatc aagatagatt
      721 ccaacatatc tgaaggctta ctttggtata ttacacgtct atttttgcat attttgcgtg
      781 tgtttcaata aactctcttc gtggaggaac ttcatcgccc atcaacatag agaatacacg
      841 gtcagcctca gctgcatttt caatagtaac ttgctttaga gttcttgttt ctggatccat
      901 agttgtactc cataattgtt cggcgttcat ctcacccaaa cctttgtatc tttgaacgtg
      961 tacgctatct tcttttcctt cgccagcaaa ttctttaata taacctaaac gttgagtttc
     1021 attccaagca taagcttgtt tgttcccttt ttttacttga tataatggcg gatttgcaat
     1081 gtagatataa ccactttcca ccaattcttt catatatcta aagaagaaag ttaaaatcaa
     1141 agttgtaata tgtgaaccat caacgtcagc atcacacatg ataataatct tatgatatct
     1201 tagctttgaa gtatttaaag ctttttcatc gccttcaaca cctttaaaaa cacccaaggc
     1261 tgtaaagata tttttaatct cttcgttttc ataaatttta tgttccattg ctttttccac
     1321 attcaaaatc ttacctctta atggtaaaat tgcttgaaaa gctctgtttc ttccttgttt
     1381 ggctgtccca ccagccgaat ctccctcgac taaatagatt tcgcaaatac tagcatcttt
     1441 ttcagcacag tcggctaatt ttccaggtaa tccactgcca gataatacat ttttacgttg
     1501 tactaattct cttgctttcc tagcagcatg acgagcagtc gctgcaataa ttactttatt
     1561 gacaattgtt ttggcttctt ttggattctc ttctaaataa tattccaaca tctcgcccac
     1621 aacaccagca acaattccat caacttctga gttaccaagt tttgttttcg tttgaccttc
     1681 aaataaaggc tccataacct ttactgaaac aatacaagtt ataccttctc taaaatcttc
     1741 gccactaatt tcaactttta catttttgaa taaatcattt tttgtacccc agtttttgaa
     1801 tacacgagtc aaagctcttc taaaacccac aacgtgcgtt cctccttcaa tggtattgat
     1861 attattcaca taagaatgaa tattttctga ataagaatca ttgtattgga aagcaacttc
     1921 aacagcaaca ctatctttat taccttccat ataaactggc tcaggaatga ttgcttgtcg
     1981 tgtcttatct aaaaactgaa cgaattcttt taagccacct tcagaataga agttttctgt
     2041 aaaaatagta ccatcatcct ttttttgtct ttcatcagct attgtaatag aaattcctct
     2101 gttcaaataa gctaattcac gcatacgtgc agctaaagta tcatagttgt attctgttac
     2161 ggtaaaaatg ctcgcatctg gcaaaaaatg tactcgagtt ccacgtttgt cagttgttcc
     2221 aacttctttt acagcatatt ttggaattcc aatactatat tcttgttcgt aaactttacc
     2281 ttctctataa acatttactt ttaaataaga tgaaagtgca ttcacacaag aaaccccaac
     2341 accgtgtaat ccacctgaaa ctttatagga atccttatca aatttaccac ctgcgtgtaa
     2401 aactgtcaaa acaacttcca aagctgaacg cttttctttt ggatgtatat ctactggaat
     2461 accacgacca tcatcttcaa ctgaaatact attgttttcg tgaatggtaa cataaatatt
     2521 tttacaatgc ccagctaatg cctcatctat tgagttgtcc accacttcat ataccaaatg
     2581 atgtaagcct ctattgttaa tatcgccaat atacatggct ggacgctttc ttacagcttc
     2641 taatccttct aaaacggtga tattgtccgc accataattt actttatttg tatcgctcat
     2701 aatctatcta aaaattgagt tacaaatata aggaaaaata agcgaaaatc cttacaaaac
     2761 aagctacttt atccacaata actattattt tatataccaa tttttatgct tatttttttg
     2821 tattattgca tattgctaaa gaattataaa aatgtcaatt actgaaaaat tatacattgt
     2881 acccaatgaa gtggatatac cacaaaactc aaatggtttg gcattgataa ttaagaaata
     2941 tgaagatgaa aaagtaaccc aaaatagcct cgaaatgcta caaaaattga tagaaggtgg
     3001 tttgagtttg aagttttcag aaactttagt attgcaaatt acagataaag agcaatttaa
     3061 gttacccaat tccataaata aagttttttt atttggtgtg cgacctcaac aaattggatt
     3121 aaatattaat agcctaagaa acaaaataat gcgtgttaat catcaatcta ttgtatggac
     3181 agatgaaacc gctttaatga taaatgatgg tgctaaaaag aaagcttttt ggattgaaat
     3241 ttctaaaatt tacaaagtat gatagcgtta gtttttgcaa ccaacaatca acataaaatt
     3301 gatgaaataa atgtggcttt gccacaaaat attcaaatta aaagtttggc ttccattcaa
     3361 tgtttccaag aattacccga aacacatttt accatagaag aaaatgctga agagaaagct
     3421 acttatgttg ttgaaaatta taaagtgaat tgttttgcgg aagatacagg acttttaatt
     3481 gatgaattga atggagaacc aggtgtattt agtgcaagat atgctggcga acaaaggaat
     3541 aatgaagata atatcaactt ggttttgaag aaactagaag gaaagacaaa tagaaaagca
     3601 catttcaaaa cagtgatagc tttgcattta gatggaaagt tatatttgtt tgaaggtatt
     3661 ttgaatggta taattattca tgaaagaaga ggctcaaacg gttttgggta tgattctatt
     3721 tttcaaattg aagatggcag aacgctcgct gaattaacga tggaagagaa atcgaaaatc
     3781 agtcatagag caatagccac tcaaaaacta gtcagctttt taaatctcta ttcaattact
     3841 aataaatcta tttaatttca ttaaaaaaat acttattttt attcgatgtt gatgcccaaa
     3901 caaaattatt gttctaattg tggaaacgct gttgtttacc aacatgctga aggtcgagaa
     3961 cgctgtgttt gtaataattg caatataatt cattatacca atccaaaaat tatagttgga
     4021 gcattaacct attgggaaga taaaatattg ttgtgtaaac gtgccattga accaagaaaa
     4081 ggtttttgga acattcccgc tggatttatg gaagatgatg aaaaaactga agatggtgca
     4141 gctagagaag tttgggaaga agcagaggct aaaattaata tcattcaacc gtacttgatt
     4201 tataatttgc cacaggcaaa ccaagtgtat atccattttt tagctgaatt gaaagatggt
     4261 gtttatggaa atggcgtaga aagtttagaa acaagattgt ttttggaaga tgaaattcct
     4321 tggaatgaaa ttgcatttta ttcatcgaca tttgcattaa aacgattctt ggaagaaaga
     4381 aaaacgggta actttaaaac tcatctagcc tcatttccag ataaataatt aggcagttgt
     4441 ttttgtattt cttagtataa aataaccaat aattcctgat aaaatggaag ccgaaaagat
     4501 gccaatctta gcttcagtag tataatgtac ttcattgaat gctaaggaag ttacaaataa
     4561 agacatagta aatccaatgc cagctaggaa cgaaacaccg accatttgtt tgtaattact
     4621 attacttggc aaaactgcaa ttttaaattt tgtaagcaaa taaactgttc caaatacacc
     4681 aactaatttt ccaactaata atcctaaaaa tacacctaag gtaacttttg atgtaaaagc
     4741 agaacttaag ctttcatcaa aagcaacacc tgcgttggct aatgcaaaaa taggcataat
     4801 gataaatgca actaatgggt gtaaactttg aataagtttt tgtagtggtg ttaatgccgc
     4861 atctgtataa aggcgaattt cactgagtat tttttgttgc tcatcagtaa caattttgaa
     4921 atgtgtacga cttgcatttt taaaatcatt gataaggtgg ttaatctttt ctatataacc
     4981 gtcttcttca atttttacgg tagctggaat tgtaaatgca gctaaaaccg ccgcaattgt
     5041 tgcatgaata ccagacatta aaaatgcagt ccataatccg aatacgccaa taattccata
     5101 aaaaatagca cttctaacgc ccattaaatt ggcgattatc attatagata gaaaaagtgc
     5161 tgcaaaacct aagctaacaa aagaaatatc tgaagtatag aaaaaagcaa taactaaaac
     5221 agcacctaag tcatcaacaa tggctaatgc tgttagaaaa acttttaatc cattagaaac
     5281 tctatctccc aataaataca aaacacctaa agcaaatgca atatctgttg ccattggaat
     5341 tccccagcca tttgctcgtt ccgtggaggc atcgtgatta aatgcatgat agataattgc
     5401 aggaacgacc atgccaccaa tcgcagccaa aattgggagt aatgcttgtt ttggtcttgc
     5461 taattctccg gcaacgagtt cacgttttag ctctaatcca accacaaaaa agaagatgga
     5521 cattagtccg tcatttatcc aatgacttaa atttttactc aatatatatt tatcaaaagc
     5581 aaacactact tgatgttccc aaatattgtg gaattgctct gaaaacggtg aattagataa
     5641 aacaagtgca ataattgcag ctaagaaaag aacaacaccg cttgcaatgg aattattgat
     5701 aaatttatta attggttcta ttatccattt atctacaggc tgatgcttca atttatccat
     5761 tactcaacaa aaataataaa aatattaaga atgaaataaa atcatcaaaa atcacaagtc
     5821 gagaggtcta ttttccttaa tttatgtttt ttctattaag gatgaggtaa ctgttttata
     5881 agttcttatt ttatagtatt tttgcaaaat gcaaaatatt cgcaatttct gtattatcgc
     5941 acacatagac catggtaaaa gtactttggc tgatagatta ttagagttta caaagacagt
     6001 ttctgaaaga aacatgcaag ctcaagcatt agatgatatg gatttggaac gtgaacgtgg
     6061 aattacgatt aaaagccatg ctgtacaaat ggattacacg catttagatg gtactaaata
     6121 tgtttttaat ttaattgata caccaggaca cgtagatttt tcttatgaag tttcacgttc
     6181 attagctgct tgtgaaggtg ctttattatt ggtagatgca acgcaaggta ttcaagcaca
     6241 aaccatttct aatttatatt tagctattga aaatggattg gaaataatac cagttatcaa
     6301 taaaattgat atggatggtg cgatgattga agaggttact gaccaaattg tagatttaat
     6361 aggttgcgaa gctgaagata ttatacatgc aagtggcaaa actgggatag gtattcagga
     6421 tattatgact gcaattatta atcgtattcc tgcaccaaaa ggcgatgaaa atgcaccact
     6481 acaagcattg atttttgatt cagtttttaa ttcatttaga ggcgtaattg cttattttag
     6541 agtaaaaaat ggttctataa agaaaggaga taaagtaaaa tttgtaaata ctggaagagc
     6601 ttatgaagca gatgaagtag gtattttaag aatgagttta gagccaagaa atgaagtgaa
     6661 ggcaggagat gttggttata ttattacagg aattaaagtt tctaaagaaa ttaaggtagg
     6721 cgatacgatt actcaattaa ataatccatg taaagaagca ataaaaggtt ttgaagatgt
     6781 gaaacctatg gtatttgctg gtatttatcc tattgataat gatgattatg aagaattacg
     6841 agaatcctta gagaaattgc aattgaacga cgcatctttg gtttttgagc cagaaacttc
     6901 attggcatta ggttttggat ttcgttgcgg tttcttaggt ttattgcatt tggaaattgt
     6961 acaagaacgt ttggagcgtg aatatgatat gaccatcatt acaactgtac cgaacgttac
     7021 ctattatgct tataccaaag ataataataa acttatagtc aataaccctt ctgatttacc
     7081 tgaaccaaat ttgttggatt atgtagaaga accctatatt aaagcgaata taattacaaa
     7141 accagaatat attggaacta taatgaagtt atgtatggat aagcgtggaa ttttaaaaaa
     7201 ccaagtttat ctaacagaaa ctagaataga attacagttt gaaatgccat tagcagaaat
     7261 tgtatttgat ttttatgatc aattaaaatc aatatctaaa ggttatgcaa gttttgatta
     7321 ccacatgata gattttagag agagtgattt ggtaaaatta gatattaaat taaatggcga
     7381 gaatgttgat gcattttcag cattaattca tcgaacgaaa gcctatcatt ttggaagtag
     7441 attgtgtgaa aaattaaaag aattaattcc aagacaacaa tttttaatag ccattcaagc
     7501 agcaattggt gctaaaatta ttgctagaga aaccatatct gcgatgcgaa aagatgtaac
     7561 tgcaaaatgt tatggtggcg atatttcaag aaaaagaaaa ttattagaaa aacagaaaaa
     7621 aggaaagaaa aaaatgcgtc aaattggaaa ggtagaagtg cctcaacaag catttatggc
     7681 agttttgaag ttgaatgatt aaattctagc aatcaaaagt attagaactt aataatttct
     7741 aaatattttt tgaaaaaaaa tgaattatat ttgcacagtt cttttatata tcctatcttt
     7801 gccgaccgaa agttctttat agatttttgt aggtgagatg ggtgagtggc tgaaaccacc
     7861 agtttgctaa actgacgtac gggaaactgt accgagggtt cgaatccctc cctcaccgca
     7921 gcaaacaatc caaccaatcg ggatgtagtt cagtccggtt agaatgcctg gtttgggacc
     7981 aggaggtcgt tggttcgaat ccagccatcc cgactaacca atagtaaaat gctttacaga
     8041 aatgtaaggc atttttttat ttaactaagt tgtttttaaa gtttactttt gttttttatt
     8101 cctcttttgt tttttatttt tagcaattct taaattttaa attatttaaa acaaaatatg
     8161 caaacattag ccacccaaga ctatatcgtt tttttactct attttatctt aatcgttggt
     8221 tatggatggt ggatttataa gcgaaagcaa aaagaacaaa cctctacaaa agattatttc
     8281 cttgccgaag gttcattgac ttggtgggca ataggtgctt cattaattgc tagtaatatt
     8341 agtgccgaac aatttattgg tatgagtgga agtggtttta aaatgggttt ggcaattgca
     8401 acctatgaat ggatggcagc atttacttta atccttgttg cagtattctt tattcctgtt
     8461 tatgcaaaga ataagatttt tactatgccg caattcttaa atcaaagata caatggtaag
     8521 gttgctatga taatggcagt gttttggttg ttattgtatg tattagtaaa tttaacttcg
     8581 atattatatt taggtgcttt agctattagt agcatttctg gtttgaacct gactttgtgt
     8641 atgatatttt tagcaatttt tgctgttatg attacgcttg gtggaatgaa agtgataggc
     8701 tttacagatg ttattcaggt tttctttttg attttaggtg gtttggttac aacttattta
     8761 gccttgaatt tagttgctga aaaatttgga atgacaggaa ttgtcaatgg tttcagttta
     8821 atgagagaac atgctaatga tcatttccat atgatatttg ataaatctaa tccaaatttt
     8881 attgatttac caggactttc agtattaatt ggcggtatgt taattattaa tttgaattat
     8941 tggggttgca accaatacat tacacaacgt gcattaggag cagatgttaa aacggctaga
     9001 aatggtatct tatttgctgc atttttaaaa ttattaatgc ctgtgattgt agtattacca
     9061 ggaattgcag cttttgtatt gtatcaaaaa ggtggttttc aggcagagat gttacaagac
     9121 ggtagcgtga atcctgacaa ggcttatcca actttgttaa atttattacc aacaggcttg
     9181 aaaggattgt cgtttgcggc attaacggca gctattgtgg cttctttagc tggtaaagca
     9241 aatagtattg ctactatttt tagtttagat gtttatgcta aagccattga taagtatgct
     9301 gatgaaaaaa aattagtttt tattggaaag ttaacgatta ttatttcgat gattattgct
     9361 gtaattgtcg ctccattttt gggaattgat aaaaaaggag gcttccaata tattcaagaa
     9421 tatacaggat ttgtaagtcc tggtgtattt gctatgttta ttttaggatt cttttggaaa
     9481 aaaacgacac caaatgctgc gttatttgcg atgataggtg gatttttaat gtctattatc
     9541 tttaaattta tgccagaatt tgttgattta tctttcttgc attcagcagg attttcagta
     9601 ccaaatagtg ttggtattta tgaaattcct tttgtagata gaatgggttt tgtatttgcc
     9661 atttgtgtta ttggtatgta ttttataagt atatatgaaa ctaataaagg tattcaaact
     9721 caaggcttag aagttgatgc ttctatgttt aaaacctcta ctgggtttat gataggttct
     9781 attataatta ttggaatttt agcagcacta tatacagttt tttggtaatt tttaataaat
     9841 cttttagact aaaaattcaa ttgtaaaaat taatgatttg gaggaggcat tgcaccgtgt
     9901 ggaggcattg caccagaagg tggtaacata aaatctttaa gtttattatc gtcatttcct
     9961 attccaactt ttttgagtgt ataggttata gttcctaaaa agtatctttg caatgctgtt
    10021 gtatgtacat cttcagtata agcatcggta acatttcgag taatacttct attttgattg
    10081 aggatatcat ttacactcaa tttaatttct aaactttgat ttttcaataa tttgtaggct
    10141 aaagaggcat tccaaagcca gtaattggta ttgtaggcat ctgagccacc agcatttaaa
    10201 gtattgctaa cttgtgatga aaatacaaat cctttccaaa attgatagtt taatctagca
    10261 gaggctgtat gattgaaata attgttgttg tttctttgtt gtaaggaatt tctaattaaa
    10321 ttataactcg caaaatacga taggttaaaa tctatttttg tactgatatt gctacttaaa
    10381 ctgatgccag cattgaagtt ataggtattg gaataattct tttcagtatt aattaatgaa
    10441 ggtgttcttt gaaaaacaaa accaccattg atattgacat tagatttgat agctttgaaa
    10501 ggaaatccat agttaattaa ggctcttgca ttccaatagc cacttaaatt tataggcttg
    10561 ataaattgca ttcctggtgt aaattgtatg ccatcaatta tcgtatcatt tgtaaatata
    10621 gttgtcgaat ttgcaactct attcatagag tttgaagtgt tggcaaagag aaatatacta
    10681 ctggcatttt cgggcttagt acgactgtat ctaatattaa gggaagaggt aaaactttgt
    10741 tttaaatctg gatttccagt tgtcaaaatt agtgggttgc tattgtctat aacattttgc
    10801 aaattggtaa tacttggagc agaagtcgaa gtcctgaatc caatatttaa gttttgagtt
    10861 tttgaaaatc tgatatttaa aaacgacata ggaagtaatg ataggaaatg cttgttgata
    10921 ttactatttt gaggtgtata ttgttcactt ttaagcaagg cactttgacc atttaaagct
    10981 atcatccaat taattttctg attgttaaat cggtaagcta aaccaagttt attcgtgatg
    11041 tatttgttgt taaattcatt acttagaatt gaatctctgg aagtataatc ttgagtaagt
    11101 agattataat tatctgtatt ttttatagaa taattatttg taaaacttgg tgttagatta
    11161 atagatatct gaccattttt cttgataggt tctgtataga ctatatttcc accgatggta
    11221 tagttattag aatttaatat agatttctga ttgatggtgt cctcattaaa aagaattgaa
    11281 tttaaagagt ataaatctct atctgtattt cttatgccat aaattgtatt gaaattagca
    11341 gagatagttc tacctttttt taggaattta tgttggtata aaatattatt tgaaaaatta
    11401 atacctaatt gttgtgcgaa ttgattgttc tgtgtagagc tttctgtaat tgaatcattt
    11461 cttagattat tcgcattaag aattgaattt gtattattgt tttgaatact aaaatttggt
    11521 gtaaaagtca aaatattaga agagtcaatt ttgtattcaa ttttcaaatt aagtcggttg
    11581 ttaaagtctt tatttgttgt gttattttgt tcattgtaag ttaggttatt atcttcttgc
    11641 aaaatataat ctctgaaagt agcgttgtta tttattgttt ttgacccatt aaaaaaaata
    11701 agaaccacta atatttactt ttttattctt tccaataata tcagaatagt ttaagccaaa
    11761 agcagttgtt gtagatattc cgcctctttg tcctactaaa aagttattta taggattgcc
    11821 accacgtgga ggtccaccac cgcctggtct aaaacctgaa tttccagtac tcaaactacc
    11881 cattaaatct tgaatgttga agttttgttg gttgatatta ttactcattc ctaaaataga
    11941 aattcttcta ttgccttgaa aagtattgta atttaatcca gtattgtaac gattgtttgg
    12001 cccaccatat ccagcataaa ttttgccaaa ttgagaagag ctaatgccac cttttgtaac
    12061 aatatttatg gtttttacac cttcaccatc atcaaatcca gtaaatgcgg attggtcgct
    12121 aagtttatca aaaacttgaa ttttatcaac aatatcagca ggaagatttt gtatagcagc
    12181 tttagtatca ctaccgaaga actcttttcc gtccactaaa acctttttta cttcttcgcc
    12241 attcactttt attgtgccat tttctgaagt catgccaggc attttagtca ataaatcttc
    12301 agtagttgca tcttgattta ctttaaaggc attcgcattg tattgagaag tatctcccaa
    12361 ttgttgtact cgtatttgcg tgctttctat caccacttct tttaatagtt tgctatcttc
    12421 tgttaattct aatgatttta aatcagtatc atgattgatt tgtaaattca attcattggt
    12481 tttataacta atatatttta agctaagttt gtaattccct ttttcaatat tttcaagtat
    12541 gaaatttcca tcaaaatcag taatggcatc ttttgtgatt gttgaatcag taatgctttt
    12601 caaaaataca tcaacaccaa tcaatgcttc ttttgtttta gcatcgataa ttgttccttt
    12661 tattgaaaaa cgatttgctg tttcttgtat ggtgctatcg tttagatggg attgttgtgc
    12721 aaaaagttgt aactgaaaaa aatataaaat gcttgctaaa atataaaacc tcattatctg
    12781 tatttaaaaa ttataatctt taagactgca aagaaatgaa gaagtttaat gggtaagtaa
    12841 tatatatttt tagattaacg aatattaacg atattgaaaa tgatagtatc tttagagata
    12901 aaatagtatt catgagaaat tggataacat cagaaaaatt agttcaacag aatcaaagaa
    12961 attatggtag gtttgagcga acgcccattg caaatccatt agatgagttt agtggtatca
    13021 aaagactttt taacaatatc agattaaaag aatgggttgg ttttgcactt tttcatcctg
    13081 atatttacgg agcgatgatt attcaaaatg caaaaattat aaagagtagt gatattcata
    13141 tttttgaaaa ttctaattat acaaactttc aaagcatttc aattagctca aaattaaaaa
    13201 tttcagaaga tttattacat tctaccttac attttaataa aagtgcttat tcgataaaat
    13261 ttgattttac agattcaaga gttgcaatat ccattcatat taaagctact aaaaataatc
    13321 aaaccataaa aggtgaaatt gtattagatg tcagtagaaa gtctaaacca ttagtagtta
    13381 gtgctttatt gccacctttg ggtacgatgt acacgaataa aataatctac ccagcttctg
    13441 gatatttgca agttggcgat aacagatatg attttttagc agaaagagat attttaattt
    13501 tagatgagca taaaagccgt ttgccttata aaacagaatg gacatgggga acttttgctt
    13561 tccctataga aaatagcttt gtaggtgcaa attttgcaaa acgtccacaa tattccgacc
    13621 aagaagaaga atcgtgtttg tggacatctt caggcgttga ggcactaaaa gacatagttt
    13681 ttgaaccaca agataatgcc caaaatgcac cttggcatat ttattcaaaa gatggtcgat
    13741 tagatgttgt cttcactcca aaaggtcaaa aagatgtccg agaaaacgct gtaatttttt
    13801 ctattaatta ttttcagatg tttggtactt attcaggcaa gatagttggt aaaaataaag
    13861 tatggaattt tgaaaatatc catggctatt gtgaacaaat gaaaatgaaa gcctaagtta
    13921 tattttttag gaattatttt aatttgaata tttttatgcc ttttaaatcg ccagacatac
    13981 cgccagaaat atttcctgta taaatataat cttcttttgt ggcaatactc atattttgaa
    14041 aatattgagg gtctagaaaa tcatcaactt tatcagattg atttggttta actaataaaa
    14101 caccactcca gccatttgcg acacaaatat aatcgtgttt ttcattatag gcaacatcat
    14161 aagcatttgt cccttcagca ctagcaaatt gatttttgaa actactatta ctgattattt
    14221 ttgtaactga aattagaaaa gggttactaa tatcaacagt tgcaatacct gcaccccagt
    14281 ttgcccaata agcataatca ttaactgtta caaaactttt gccccatttt tcatgccctg
    14341 attcattcgt ataagttata gtagttgccg tagatggaaa attggattgt ttgaatgtta
    14401 taaattctcc caatgcattg gtcgctagaa tatacccatc ttcctcttct aaccactcaa
    14461 aattactctg tgcagaagtg tgttgccatt ctttatcaat atttaatata ttagcagata
    14521 aagtgcattt ggctacaatt ccatttgcac cattgcttcc acctacccaa aaattattat
    14581 catcatcaat tagtaaaata ttaggtttta gattactaga actaaaagtg tatttaactt
    14641 caaagttgag gtcttcatca aatacataaa ccgctttttc atcgatgtta caaacgtaga
    14701 gtttgtcatt atgaaaagtc attttaccaa agtttttgtt ataggtctgt gtatatttat
    14761 tttgttgttg tggtacttgc gggttattca atgaaaatag gaagatttga ttatctcgtt
    14821 ttgcatataa gttattatca tcaattagca attgatgagg tccttccgaa agtgcgattg
    14881 aacctacgaa atcaattttg ccatttacag actgctggtc ttttttcggg tcaggctctt
    14941 ttgttttttg agatttttct tcttttgtgc aagaaaagaa cagtataaaa actgcaaata
    15001 aaatcattaa atttttcata atgtaatttt taattttgtt tagtaatagt ttagttctta
    15061 ttaatattta ttctaatccc aataaatata ctcatgaata aacatactta aaaaatccca
    15121 aattataaaa taaaataatc aatcttatgc ttcaaccaat agaaatcatt aggcgaaatt
    15181 ttatttttaa gttttcaagt aaaagctgtg tctgttgaat actaagcttt tataaagatt
    15241 actaaagagt ttaggtaaca atgcttaatg cattgctttt caaagtattt tagtttactc
    15301 aaagcaaata taaaagaaac cgattcatta tctatttaga tagtttttgc ttttcatatt
    15361 tacgttgaac attcctaaca gttgatttta aatcatcaat caagctttga ggtttaagta
    15421 ctttaagatt ttctccaaag gataaaagtt ccataaagaa atcatgtgta atgaatagtt
    15481 taagctgtat tcttaattca tctttattat ctatcagaat ttgctgagag tcgtgtaacg
    15541 gtaatgattt gatatatttt ccttgaaatg tattgaaaga aagtattaca tcttctggtt
    15601 gatgaccatt cggactaata ataccaaaac agtatctgta atgttcattt acatcaaaat
    15661 tagttgggaa gttgaatttc tttttcaaaa tttccaaatc tgttaaacgg tcaagagcaa
    15721 aactctttat tttattatcg cctaaatctt ttgctaagac ataccatctg tttttgaatt
    15781 ctttgagtgc ataaggttct accaaccttt cctttaatac ttcttcccaa tatttttgat
    15841 acacaaattt tatttgaaac tgatttttaa tagcatgaag taagccgtat aaattttctg
    15901 ttccctgtgg tctgcgtttt tccaagtgaa tgaaaggcgt taagtcttgt gcaagattta
    15961 atgaattgaa catatcaaaa gcttcaatca ttctttgaaa gttcatattt tctgtttcgc
    16021 tttgactgat gaaataacct ttatttgctt ttgaatattc aatatcaatt ccaaaaacgt
    16081 ttctgatttc ttttaaatct cgttggagtg ttcgttttga aaaaccaata ttcaacgtat
    16141 cgtcctgcat ttgcaaaaaa tcaaattgat tttcaatgta ggtttgtaat tccacataag
    16201 ttgaataggg ttttgctttc aactttttta gaatgagcaa gtatcttgat atgtagcctc
    16261 gttttgacat aactaatttg atttgttgtt ttttaaattt ttatcaaaaa taaatattta
    16321 gaacgacaga ttgtgtcgct ttgaaattta tacataaaaa aacactcgaa aggtctatga
    16381 ttcgggagtt gaagcagaga atgtttttta tttcctatat cgttctgtac ctattacacc
    16441 taattctttt gctttttctt gccaaacaat attatgtcca tattcattgc cgacaagtgc
    16501 gtgggcaatt tcgtgtaaaa tggtgtttct aatttcgtcc atatcattat tgattgcgtg
    16561 attgatttgt atagaaatta tttctccgct gtttacacat ttacccaaag ctgaatgatg
    16621 tatccgaaac tcaatctttg tatttcttag attatgtttc aacaaaagtt gatttgctaa
    16681 aaaaagaacc tccaacaatt tcatattagt tacatcatca aaagcgtatt tatttgaaat
    16741 aacatttttg agttcttctt gtatcctatt cattcctata actttttttg ctatttttcg
    16801 gattttggca ctgagttatg aattaaatcg ataaattttt gttcattaat tttatcatca
    16861 aatataattt tacctaagcg aaccatattt ctaatttgtc gtccgtttaa gtttattttg
    16921 attaactcgt ccatcgaatc gatatacaat cctgcacttt cgagtttaga tttccaaatt
    16981 aattttctca catcatcggt aaggtcggga tatttaatat ttaaggtaat tctcgaaaga
    17041 acagcatcgt caattacctc gcttctgtta gtggtaaaaa acatcattcc tctgaaataa
    17101 tccattagcc ggagaaagat tccgacaata gcagattttt caatattatc tcctcgtttt
    17161 gacaagaata catctacctc atcaaacaga atgatggcgt tccatttttc tacacgccta
    17221 aaaatatgat tcagattttg ctcaatctta tcaggagaaa taccaatttc atcaacctga
    17281 actatgtaaa gtggtttttt attcaattct gaatatactt cggcagtcga ggtttttccc
    17341 gtaccggaat ttccttctgc cataataatc agtccgccgt gtttattatt gataatatcc
    17401 gttgttaaat tttcgaccga ataagaaaag actttttcta aaatgttttt tagattcttt
    17461 ggtaggaata atttctgaaa tgcctgttca tcatattcgt aatctgaaat atcatcaatg
    17521 tgtacatata catattcttt gtacaacaac gaaaacacac gaacataagg aagttctata
    17581 tggcttttaa aactatcatt ggtatatcgg ttatttactt gaatttccaa ttctgactca
    17641 acaatacagc tgctttgctt attaaaagaa cccatattag tcaattcaaa atgggaagac
    17701 aatcctaatc ctttacataa gatttgttta ctatcttgct tttgggtttt cttggtttct
    17761 acaattctat tttcgtattt ggacaaattt tcttgttgct caaaaacatt aaatatcttc
    17821 atcaaatctc tgaacgaaag agtactcatt tcttttctta aagaaattat attgtcattg
    17881 ttcagttcat aatttagact gtgtgtgtct ttatatattt taaaatcgag gcgaaataat
    17941 attcgctttg gagaatgtct tgtggcatta agcaattctg ctaccaattt tgctggattt
    18001 tttcttctaa aaattaagca atcaacatta tattttattt tcttcaaggt ttcataaacc
    18061 tccataatat cactaagact ttttatcttt ttatcccaaa tatttgactc tgtatttgag
    18121 ttttggacaa catcattctt caaataattt agtgtgattt ctgctttagt ttgcagattg
    18181 atttgggtat cgttagtcaa aatatattcc aacagatttt tggatatttt ttctaaatct
    18241 agttctatat tttcatcttc acttgtttcc aattggttta aagaatgtac atcattttct
    18301 tcaaaagaat tttcctcaaa ataacgctct aatatttctt tgccgatgat aattgtattc
    18361 attttttaga agttttaatt atttaatact cctgcaaaga tgaatcatta ccatgccaaa
    18421 ccgtgtcgtt gtaaaaacag aaataaaaaa ataccgaaag ggtaagattt cggtattggg
    18481 gttttgtttt tatttttttt attatgagat tataccttgc tattgactct ctttttgaca
    18541 taattgatga taaatagatg cgttaatata ctgaattttt ttatgctttt cttttccata
    18601 gttggaatga ttatgaccaa atacgtggta ttttggcacg agctgaaata caatattact
    18661 aatttctttg ctgccaaacc cattatctaa aataccttga ggaggataat gagatgcaat
    18721 gatgtcaatt cttttttttg ttacaatgcc attttgaaaa gggaaagcac ttattcccat
    18781 tatatttata tcacttatta caatcgattc atcatttaac catataatac ctttaggaat
    18841 taatctttta ttccattcag gttctaactc gaaaggtaaa tcgtgatttc catgaataaa
    18901 tagcttgtgt ggtatttcta attgagcata ccaatcaaaa aaatctaaaa tttcatctat
    18961 atctccatca ttacaaatat ctccacaatg aattacaatt tgaatatttt taggaatttc
    19021 gattaagcga tgtttaccat gcgtatctga caacaataat atattttggt tttttatttt
    19081 aatcgtgtgc atagctaaat ccaattttag ttgtttttgt tataataaat tcttctttac
    19141 aaaatgattg tatttgttca aaattagtta ctgagccatg aacaatttcc tgtatttcac
    19201 ttttgcgaat aatattatct atctgtccac cagaaaagtc aaacttctct gcgagtattt
    19261 gacaacactc agcttctaaa tgtggtaatt ttgatttcca tattttagct cttacctctt
    19321 ttgtaggttt cttgaaaggt attttgaata aaaatcttcg ttcaaaagca gtatccaaat
    19381 tatttgctaa gttggtcgtt gccattaaaa tgccttcaaa gttttcaagc tcttccaata
    19441 taatattttg aatagcattt tcagtttgag ccacaccgga attactattt tcctttcttt
    19501 tagaaataat tgcatcggct tcattaaata gtaaaatagg cgttagctta gattcttttg
    19561 cataagctct gtaatccgta aaaatacgtt tgatgatttt ttcactttcg ccaaaccaca
    19621 tagattttga ttggctgatt tctactttca taatttctcg gtttgtcgtt tttgccaatt
    19681 gtttcacaat ttcggttttt cctgtgccag gtacaccatg taacaaaatt gcaatacctt
    19741 ttggtaaacc tttattggtc aaccttgtct gtgtttcatg atatttatct tcctgaagta
    19801 aattttgaag tgtttctaat tgactcatct cctcattatc aaaaatcaat tcacgataaa
    19861 ctactgccga aggaacaata atattatctt ttttcttttt gtttagaaaa agtttgatgc
    19921 cacaatcatt caaaagttga agagaagtat ccgtcaattt catttctgta tcattgaaaa
    19981 attgagcttc tataatttca accaaattat tgttagtaag gatatgattg cctgacaaca
    20041 atttttgtat ttcatttact ctctctgatg gactatcata aatactctcc aaagcttttc
    20101 caatatctat agtttctttt cctgacactg ttttccatat tatatacaaa agaagataag
    20161 tatctttagt gccgaacttg aattgtttta ctttttctat taacgaaaaa tgcatattct
    20221 ctaaaatcaa tttttcaact tccagaaata gttctaaagt tgaaatttta tcatcgtctc
    20281 gttgatcgcc caatttgtat aactcttcta acaaatctat tatactcttt attttattct
    20341 cgttgatttt gggcaatggt ttattctgca aaatggcttc agtgattttt tcgtgaatag
    20401 caaattggtc attagctcct gccagtttca aacggtgtct agatttcttt ttttcaaaaa
    20461 tcccaacttt gtgtaagtag tcaaaatcat cgctgtattc caatattttc attggattac
    20521 aatcaaaata ctttatcaaa tcgtttaaat caacggtgtc tcccttgtaa tttaaagcaa
    20581 aaaccattgc aataaaaaaa gtttgatgct cggatgtttt gaaatatttg gaaagtgtat
    20641 atagttcagg ttttatactt tcaaaaaatg aatttccaag cttacacttt tctgattgtt
    20701 cgtacacttt cccaatgctt tccagaattt gtttctttgt gatactttcc ataaacctat
    20761 cttttttgac aaaggtagat ggaaggtaag acaaagtgtg tcgtggtt
//
LOCUS       DAOQCW010000033        20647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_934_length_20647_cov_6.093434, whole genome shotgun sequence.
ACCESSION   DAOQCW010000033 DAOQCW010000000
VERSION     DAOQCW010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 20647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20647
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_934_length_20647_cov_6.093434"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PLS16_04465"
     CDS             complement(<1..241)
                     /locus_tag="PLS16_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60365.1"
                     /translation="MTFVNNPSDDDLKQMFGYNLHWKAEKSLLLYPKTNQMDSKFGIF
                     HYDNLGKKCKLGFVDITKEQSIKNSQIIAYEIFDKL"
     gene            complement(295..1728)
                     /locus_tag="PLS16_04470"
     CDS             complement(295..1728)
                     /locus_tag="PLS16_04470"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNJ60366.1"
                     /translation="MINEEYLENSEYISYGDFTEEQFYTLAVETIKKLNWEISIIDED
                     GLTTTSINSKSDESEFCIVLGDENVELNSIANKSEQTNFDNIEQFKTVFYQLKATFSK
                     EEIEEKQQQLYEQIGEKVIEEDTSTEDENNGHWWDVFMPVKDYFFTPIIININIGLFI
                     LMLLFGVKLLNPYNDDLIKWGANYAPYTINGQWWRIVTSNYIHIGIWHILVNMYVLLI
                     FGATLESIIGSKKFILVYLISGIFGSLGTLYFLPWTVSAGASGAIFGIIGTYLALATT
                     KLFDKKAKKEVLPTLILYVIISIIPDSETNIYAHAGGLFTGLIIGYFYSYTFPHIESE
                     KKENLILIGLVGIYFTLGILIFQKAPNPSKYYDEQFKIFREQEDKALNIPDLETVKSQ
                     LYIEYLQDTSIYYWNKNIQLLNQLDTNKLPKWIIDDNNLLKEYCNYRIKEFQYIILNT
                     KKETSNYNDSINFYGQKIETLLNTYNN"
     gene            complement(1853..2776)
                     /locus_tag="PLS16_04475"
     CDS             complement(1853..2776)
                     /locus_tag="PLS16_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNJ60367.1"
                     /translation="MSLLIVGTVAFDAIETPFGKTDRITGGSGNYIAHSAGHFTHDMG
                     LVSVIGDDFDQDELAYLKKIGTDISGIEVIKGEKSFFWSGKYHLDLNRRDTLATDLNV
                     LANFNPVLPEHLRKPDFLILGNIDPALQIKVIEQLEARPKLIAMDTMNFWMDIALENL
                     LSVLTKVDLLTINEEEARQLTGEYSLVKAAQKILTMGPKFLIIKKGEHGALLFHGNRV
                     FFAPALPLEDVFDPTGAGDTFAGGFMGYLAKTKDISFDNMKRAVIYGSALASFCVEKF
                     GPQRLKEVTQEEIEDRVQVFIEIVDFDIILE"
     gene            2854..2927
                     /locus_tag="PLS16_04480"
     tRNA            2854..2927
                     /locus_tag="PLS16_04480"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2888..2890,aa:Asn,seq:gtt)
     gene            complement(2968..5640)
                     /gene="metH"
                     /locus_tag="PLS16_04485"
     CDS             complement(2968..5640)
                     /gene="metH"
                     /locus_tag="PLS16_04485"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNJ60368.1"
                     /translation="METDCNILKLSGLEPLIVTKESNFINIGERTNVTGSKVFLRMVK
                     EDRYEDALAVALDQVRGGAQVLDVNMDEGMIDGVKAMTTFLNLIASEPDIARIPIMID
                     SSKWEIIEAGLKCVQGKSIVNSISLKSGEAEFIHQAKLIKKYGAATVVMAFDEDGQAD
                     SYDRRIQICKRSYDILVNEVNFNPNDIIFDPNIFPVATGLDEHRNNALDFFNAAKWIR
                     ENLKGAHVSGGVSNVSFSFRGNNAVREAMHSAFLYHGIQNGMNMGIVNPSMLEVYDSI
                     DKTLLEYVEDVLLNRKDDATERLLEYAEKVKGGGKKKEEDLEWRNQDVKERLKYALVK
                     GLVEYIDEDTEECRKLFDKPLEVIEGPLMDGMNIVGDLFGSGKMFLPQVVKSARVMKK
                     SVAYLQPFIMASQSGESKKLGKVLLATVKGDVHDIGKNIVGVVLACNNFEVIDLGVMV
                     PTEKLLDEAIKQNVDIVGVSGLITPSLDEMVDVAKEMERRNFKLPLLIGGATTSKLHT
                     AVKIEHHYSGFTIHVLDASRSVPIATQIMNEQDREKLQKETRAEYVAMRDRHNSKKET
                     KEYLTIENARKNKTQIDWANYTPPKPTFIGTRTFVDYDLAELRNYIDWTPFFQVWELH
                     GKYPTILEDKIVGDEAKKLFADANAMLDKIITEKWLTAKAVIGFFKANSINDDDIEIV
                     ANNKIKILHHLRQQNKKAAGLPNFCLTDYIAPIESQKEDYIGGFAVTAGIGIEKKLEE
                     FQANNDDYDVILLKGLADRLAEAFAERMHERVRKEFWAYDKTENFSNEDLIKEKYTGI
                     RPAPGYPACIDHTEKNTLFEILDATKEAEIILTEGMAMYPTAAVSGWYFSHPDSKYFG
                     IGKIAKDQVVDYAKRKDMPLEKMERWLGSALNYQ"
     gene            complement(5687..6685)
                     /locus_tag="PLS16_04490"
     CDS             complement(5687..6685)
                     /locus_tag="PLS16_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNJ60369.1"
                     /translation="MSNNLYEIAKQKILILDGAMGTMIQRYKLEEADFRGDRFKEHQH
                     PLKGNNDLLSITRPDIIKEIHRQYFQAGADIVETNTFSGTTIAQADYHLEDAVYDINF
                     QSAKLAKEVANEFTDFPRFVAGAMGPTNRTASLSPDVNNPGFRAITFDQLVTAYHQQA
                     KALIDGGVDILLIETIFDTLNAKAALFAVQTLFEEMGRELPIMISGTITDASGRTLSG
                     QTTEAFLISMSHVNLFSIGLNCALGPRELRPYLQILAKSAPFMVSAYPNAGLPNAFGG
                     YDETPEQMTDAVKEYLEMGLINILGGCCGTTPQHIKAFAEAAKAYKPRQILTESVC"
     regulatory      complement(6779..6911)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            7094..9115
                     /locus_tag="PLS16_04495"
     CDS             7094..9115
                     /locus_tag="PLS16_04495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HNJ60370.1"
                     /translation="MHFVFPTFLYALIALSIPIIIHLFYFRKYKKLYFPDIRFLKKLQ
                     EQKSNINQLKQRLILLARLAAIFFLVLAFAQPFFGKKETLNPANTYAAVYIDNSPSTQ
                     LIKDGETILDIEKNQAKQVANALNPNDKIALITNDLYNSYQRWMNKQDFINAVDNIKS
                     SKQQKSLEEVFLKQNALLESAGSNPKNRFIFSDFQTYIIDNLKIDTNFTTYLLPIQTK
                     GENNVFVDTCWLGSPVVQLNTNNKLFIRFKNSGEKTVTNKSVTLKINEQVKSVKDINI
                     AANSYSVDSFTFSIQKSGVQNIEVVFNDYPITYDDAFYASFEVKQNEHILSIEDVEST
                     KHISAIFLADNYFTYTKNDVRQIDYSKLASYNFIVLNQIKEISSGLINSLKIYIQNGG
                     ALFVIPAKDINLESYNVFFNTLGIGTFSAIKTQKSEVRKLNLNEKIYQGVFAQIPKNI
                     DLPKIEKFYPLNVNAQTMHYDIMSSTTNTPLLSKFIVDNGIVYIQSTPLDISFSDIGT
                     KAVYAPIVYNMAILKNYAPPLFYTIGKDILIKIPALQNNHQEQVSITNQTQEFIPESR
                     MLGNTLSLNVKQNLERDGFYKIKIGNHTILGNIAFNFNRKESDLNFATIENLESRFDF
                     PNFKILEQKNSNIFAQVKHIKDGTVLWKIFIVLALLCLLIEILLIRFLK"
     gene            9123..10385
                     /locus_tag="PLS16_04500"
     CDS             9123..10385
                     /locus_tag="PLS16_04500"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNJ60371.1"
                     /translation="MKILILQVTILDEGSPYHQQKVDILLENGIIKNIQSKIEKTNDF
                     EIHNFEGCYLSVGFCDLITQIGEPGFEYRDDVESVAKSAIFGGFTTICALPNNHPITQ
                     NKSDVEFILNRAKSTAIHILPFAALTKDFNGKSPTEMMDLNKAGAIGFTDIPHNIKDN
                     GVLLRALQYTQQFGGVVFALPFDKNLVHDGQVNESIVSVKLGFRGIPEISEYLAVYQS
                     IEILKYAGGKLHLTGISSEDSIKLIAQAKKDGLNITASCFVHHLISTEESVATYNSNF
                     KVFPPLKNDKTRLALIEAVKNKTIDVISTQHTPLDSEVKNLEFEYADYGMIGMETALG
                     LLLKYTDIPLEILIDTLSYSPRKIINRQLTIENDKIGEFTIFNPSEEWNFEEQNIQSK
                     SKNSPYIGKALKGKVKAIFNKGVLTQIQ"
     gene            10389..10841
                     /locus_tag="PLS16_04505"
     CDS             10389..10841
                     /locus_tag="PLS16_04505"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HNJ60372.1"
                     /translation="MKILMVCLGNICRSPLAEGILKDKCKKKGLNWEIDSAGTGRWHV
                     GSQPDKRSIEVAKNHGIDITDQSARSVNSNDYSYYDIIYAMDTMNLRDLKAWAVDANE
                     EGKIRLILNEIDSFPTESVPDPYFNDNGFEAVFNMLDKACDAIIKKYG"
     gene            complement(10857..11021)
                     /locus_tag="PLS16_04510"
     CDS             complement(10857..11021)
                     /locus_tag="PLS16_04510"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNJ60373.1"
                     /translation="MEQLNYSQNDLAEVIGLKSRASEILNKKRKLSLEMIRKLTEKLH
                     IPSEVLIQAY"
     gene            complement(11085..11213)
                     /locus_tag="PLS16_04515"
     CDS             complement(11085..11213)
                     /locus_tag="PLS16_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60374.1"
                     /translation="MKLKPIKTEQDYFKALERLELIFDAKSGTNEGDELEILGILL"
     gene            11770..12861
                     /locus_tag="PLS16_04520"
     CDS             11770..12861
                     /locus_tag="PLS16_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60375.1"
                     /translation="MKANLLRNISYALVLLLTFSSCSKDYERIKDPIEEETPVTIYIS
                     GYSNEIANMGEVVWKNGKPIQVYKTGVLPSGYVRYGGIIYDFDIENDNVYAVWKLSHK
                     KLSAYFPSAHIYLWKNNENTLLLQNDYNIYPKAVDKHGDDLYFAGDYTTPQSYPSIWK
                     NGNREQLPGGIGSVSDLLVTNTDVLAVGYVVTGSQYAAAFWKNGVLTKLNEGFSYTAN
                     SIAQAGYDTYIAGSGKAGVNDPLLALIWKNGIQQTLLLPAGQIGASSNSVAIGGNKVY
                     VVGYSYTADNDSEKAVLWIDGSPLILSSTKSIAYSVVVKGNTIYACGYEKSSTKQRAV
                     MWKIKDGNIETTKLSNGTEDAVATCIKVK"
     gene            12889..14184
                     /locus_tag="PLS16_04525"
     CDS             12889..14184
                     /locus_tag="PLS16_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNJ60376.1"
                     /translation="MKTKIILIGLALAIGFSANAQIINPKETTKQKVEDRTNRKVDEA
                     IDKGLDKIEDGISNIFKKKDKKQKDNSNDHSQTDNTNNSDTNQNTTQNGNTDFSKYKN
                     FDFIPGKNVIFFDNFSDGSKARWKAYDENQLSISANQESNWLEMGGTRFAPINVGVLP
                     EDVTIEMDAYVRNGKQPCGTLEISFVEQSEKDRLEDPSLDNSSTISISPITETPKNAT
                     ISYEKKKNNEVIGYTMGFGFKTWQPELGNTHAHISIARKGNSVSVYINNEKLLDNIDL
                     LNSDMKYMLAMNLQNYFVEETRMYITNVRIANGTSNAVSEVKLKGKFVTNTIYFDVNS
                     SHIKPESWVTLKQTADAIKANSGNFLIVGHTDSDGADDVNLKLSQSRAASVKNALVKE
                     FGIDASRLITDGKGENQPVSSNNTALGKAQNRRVEFIME"
     gene            14264..14836
                     /locus_tag="PLS16_04530"
     CDS             14264..14836
                     /locus_tag="PLS16_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60377.1"
                     /translation="MSVPDDFSSEKIISKQNRLQQILTKEMVAKAVNVPIEKIEISVE
                     NSINRSGQYTALFSWTTGDKKRVGNHEIDEYHSISIGFIKQMNIDDFEKYYGTNEGLQ
                     MEVDNMSKQEYFNKEIGTAEAKYISEYARTRKMQKLDNVATVAFWETPMNALHVLAND
                     ATFTVTVNFGDDEVLAKKKSVELTNAILNN"
     gene            14849..15763
                     /locus_tag="PLS16_04535"
     CDS             14849..15763
                     /locus_tag="PLS16_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60378.1"
                     /translation="MRIYFLIIGMLISVSIFAEKAIIANCSEGGGCTFELSDIESDLL
                     DNDFGRNVQPDDIIVLGKKKNGEVITYLDKKSRAAIDRDWGGDGYTQLGLFNPQIQPL
                     NGMWNVVYGSSTGNDCYGIGNIGSFFRKKITPGTAGNGNIKFQFPFNPSQLFPANDMR
                     WVKTGYNTYKGLLDFNSNSMGGMKMYYHVTIFSNKKIETIYTIEIKVPTKPLCVGKVP
                     VTYTLLDAEETEFPEDVESDDDLLPVNPKGKKQEDDLMPINPKGNENDLLPVNPKKED
                     LLPVEPEKKNKTKVERLEDKPKVPRLEN"
     gene            15775..16116
                     /locus_tag="PLS16_04540"
     CDS             15775..16116
                     /locus_tag="PLS16_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60379.1"
                     /translation="METNTQQSITEGKSTAIIAYITVIGLIIAFVQNSEKKNTFANFH
                     IRQSLGLMCTGFALGLVNIIPILGWIVSVFGMIVLLILWISGLLNALNGKESPVPILG
                     NLYNKWFSGIQ"
     gene            16142..16924
                     /locus_tag="PLS16_04545"
     CDS             16142..16924
                     /locus_tag="PLS16_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60380.1"
                     /translation="MKQIQSIVTFAAICITLYSCGNGENKSQNGNDVNIDAVINSKSK
                     DSNAKGDNKCLLEYQNKYDALLSENDVIKATGFSKEKMETQYNKVLKNAEHHEFLYKF
                     KNGRVGKVRGLDREMEQPDVVSIRSIKSMSLTTFNQSYQAITDEQMETANDVIDEIAE
                     GNSGDADADKALQKAKEQNVSKDQIKKTGGILTGAFKEVSEGYRAVEGLGDAATWNVV
                     TNELIVLQNGVKFEILADISNDNEQNKSVAIKLAEIVLNKCK"
     gene            17192..17728
                     /locus_tag="PLS16_04550"
     CDS             17192..17728
                     /locus_tag="PLS16_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60381.1"
                     /translation="MAIYREPILNYWLLTTEAYWQTDEMSLWFILNPNGEVLQAYQDG
                     ESNSSKRLMKHSLYPDKNTVLPNNWKATGITKSFGDVSLGFPKFIGEEYNVSYDKTNE
                     QSVFFLTPTKADFSTLYLFNGLDIDAKLPIRFPKDIPSNYVVLSEETKFSNVSELYRF
                     KYISHTDYHINISEYNLK"
     gene            17867..18487
                     /locus_tag="PLS16_04555"
     CDS             17867..18487
                     /locus_tag="PLS16_04555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ60382.1"
                     /translation="MKILLADDHFLVLEGLEVLLSTFDFVKSTQSVINYMEMKEVLKH
                     ETFDVLLLDIHFGKHDGRVIITEIKKMMPAIKIIALTSHSDATTVKSSFNAGFDGYLL
                     KIDNREEIEKALKAVSSNEKYFSSKTQQVFFENQVSKNKVALTEREKEILQLIVEEKT
                     TKEIAEQLYLSEKTIETHRGNLMIKLDVKNIAGMVKKAIIQGLVNI"
     gene            18500..20374
                     /locus_tag="PLS16_04560"
     CDS             18500..20374
                     /locus_tag="PLS16_04560"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNJ60383.1"
                     /translation="MRIYVLLFLLATLMQSCSNSGTNQTTKNAVSSEDSLQIMNAFVP
                     PFINADSIYNSLQTDFSKKAATIDFLNLNYGKVLIMSGRLDKADTLITSAITRTGFDT
                     TSLAHARYANLQAAIEAYKQNQEGAIGYYKKAIQLFEKHNDTKSAASINFNIANIFLS
                     RLNYPMAYQYSAEAVKGFKAANDTLYYPSALAVNAVSAIVLGKKEEAKTTAEQAREIS
                     ERYKNALGIVMSSYALAEIAMYDKKYDTAIAQFQKVIPLAQQLQQITVTSAAYTSLVK
                     AYLESENYDLAITEGKKAIEFSKKFGYEDVSYSLNRYISQAYQQKGDEHSALQYMRMA
                     DEHFRDELVSNDRRVMGELLVQYQTEKKERQIAEQTLEIQNHRSNFLYAIFGGVLLVL
                     VLGGFFIYNRKAQKLKLKRLQQEKENAILNSFILGEERERKRISQDLHDGVAAMIGAA
                     KMSLDAIPHLPEDKRLEQLSKVKGILENTHADVRHIAHNLLPTVLEVEGLIKATTHFA
                     FEINQTKLIDISVKDYNSNAKDLSTQLQLMLFRVIQELVNNIVKHSQAQKAEIIFSNS
                     PNGLQIEITDDGIGYEGINNSGNQGLYSITQRLKSIGGNFKITKGSSGGTQAKVEIVV
                     "
BASE COUNT         7166 a         3470 c         3430 g         6581 t
ORIGIN      
        1 ttaacttatc aaaaatttca tacgcaataa tctgactatt ttttattgac tgctctttgg
       61 ttatatcgac aaagcccaat ttgcattttt tgcctaaatt gtcataatga aatattccaa
      121 acttgctatc catttgattg gttttagggt aaagaagcag tgatttttcc gctttccaat
      181 gcagattata accaaacatt tgctttaggt catcgtctga tggattgttt acaaaagtca
      241 aaatatgctc attaatattt tattgcttta aaatttccaa aattctaata taaattagtt
      301 attgtaagta ttgagtaagg tctctatttt ttgaccataa aaattgatgc tatcgttata
      361 attgctagtt tctttttttg tatttaaaat aatatactga aactctttga ttctataatt
      421 gcagtattcc ttcaataaat tattgtcatc aataatccat tttggtaatt tgttcgtatc
      481 caattgattt aaaagttgga tgtttttatt ccaataataa atgctagtat cttggagata
      541 ttctatatat aattgagatt ttacagtttc taaatcaggg atattgagtg ctttatcttc
      601 ttgttctcta aatattttga attgttcgtc gtaatattta ctaggatttg gtgctttttg
      661 aaatattaat atgcctaaag taaaatagat accaactaaa ccaatcagaa ttaagttttc
      721 ttttttttca gactcaatat gtggaaaggt atatgaataa aaatatccga taattaatcc
      781 tgtaaataag cctccagcgt gtgcatagat gttagtttct gaatcaggaa taatacttat
      841 aattacatag aggattaaag ttggtaagac ttcttttttt gcttttttat cgaatagttt
      901 tgtagtagca agtgcaagat aagtaccaat aattccaaag atggctcccg atgcaccagc
      961 agaaactgtc caaggaagaa agtatagcgt ccccaagctt ccaaaaatgc cgctaattag
     1021 atagactagg ataaattttt tacttccaat gatgctttct aaagtagcac caaaaattaa
     1081 gagtacatac atattgacca aaatatgcca aattccaata tgtatataat tactggttac
     1141 aattctccac cactgaccat ttatagtata gggtgcgtaa ttagctcccc attttatcaa
     1201 atcatcgtta taaggattga gaagtttaac gccaaataga agcattagaa taaataagcc
     1261 aatattgata tttattataa ttggcgtaaa aaaatagtct ttaacgggca taaaaacatc
     1321 ccaccaatga ccattgtttt catcttctgt tgaagtgtct tcttcaataa ctttttctcc
     1381 aatttgttca tataattgtt gttgtttttc ttctatttct tctttagaaa aagtcgcttt
     1441 caattggtaa aaaacagttt tgaattgttc aatattatca aagtttgttt gttctgattt
     1501 attagcaatg ctatttaact ccacattttc atcgcctaat acaatacaaa attcagattc
     1561 atcacttttt gagttaatag aagttgttgt taagccatct tcatcaatga tagaaatttc
     1621 ccaattaagt tttttaatcg tttctacggc taaagtataa aactgctcct ccgtaaaatc
     1681 tccgtacgag atatattcag aattttctaa atattcttca tttatcatat agtcttacgt
     1741 ttgatggtac tcaaatttaa aactttaatt aaaataaaga aatttataga caaatccata
     1801 tatcgtgaaa gtgtccacat ataagtactt aattaaaagt agaaattgtt tattattcta
     1861 atataatatc aaaatctacg atttcaataa agacttgtac tctgtcttca atttcttctt
     1921 gagtaacttc ttttaatcgc tgagggccaa atttttcaac gcagaatgac gctaatgctg
     1981 aaccataaat tacggcacgt ttcatattgt cgaaggaaat gtcttttgtt ttagctaaat
     2041 agcccataaa tccaccagca aaagtatctc cagctccagt tggatcaaaa acatcttcta
     2101 atggcaaagc aggtgcaaag aaaactctat tgccgtggaa caataaagca ccatgttctc
     2161 cttttttaat aattaagaat tttggaccca ttgttaaaat cttttgagcc gcttttacta
     2221 atgaatattc gcctgttaat tgtctagctt cttcttcatt aatcgtcaat aaatctactt
     2281 ttgtcaaaac gctcaataaa ttttctaatg caatatccat ccaaaaattc atggtgtcca
     2341 ttgcgattaa ttttggtctt gcttctaatt gctctattac tttaatttgt aaagcaggat
     2401 caatatttcc gagaattaaa aagtctggtt ttcttaaatg ttcaggaagt actggattaa
     2461 aattagctaa aacattcaaa tctgttgcta atgtatctct acgattcaaa tctaaatgat
     2521 attttccact ccaaaaaaat gatttttctc cttttattac ctcaattccc gagatatcag
     2581 taccaatttt ctttaaatag gctaattcat cttggtcaaa atcatctcca attacactta
     2641 ctaaacccat atcatgtgta aagtgtccag ctgaatgggc aatataattg cctgatcctc
     2701 ctgtaattct atctgttttt ccgaaaggtg tctctatcgc atcaaaagct actgttccta
     2761 caattaacaa actcataaaa aaatattttt cacaaagatt gcataattat tttaattttt
     2821 atatctttgc ggtcgttatt aattaaatga cacgcttcct tagctcagtc ggttagagca
     2881 actggctgtt aaccagtagg tccttggttc gagtccaaga gggagcgctc caaaaaaaag
     2941 aggttgaatt ttcaacctct tttttattta ctgataattt aaagcacttc cgagccaacg
     3001 ttccattttt tctaatggca tatcttttcg cttagcataa tcaacaactt ggtcttttgc
     3061 aatttttcct attccaaaat atttactatc tggatgtgag aaataccaac ctgaaacagc
     3121 tgctgttggg tacatcgcca ttccttctgt taaaataatt tctgcctctt tggtggcatc
     3181 taaaatttca aacaaagtat ttttttctgt atggtcaata caagcaggat agccaggtgc
     3241 tggtcgtatg ccagtatatt tttctttgat taaatcttca tttgaaaaat tttcagtttt
     3301 atcataagcc caaaattctt tacgaaccct ttcatgcatt ctttctgcaa atgcctctgc
     3361 caatctatct gccaatcctt ttaatagaat gacatcataa tcatcattat tggcttgaaa
     3421 ttcttctagt ttcttttcaa ttccaatacc agcagttact gcaaatccac cgatataatc
     3481 ttctttttga gattcaattg gagcgatata atctgtcaaa cagaaatttg gcaaaccagc
     3541 tgctttttta ttttgttgtc ttagatgatg taagatttta attttattgt tagcaacaat
     3601 ttcaatatca tcatcattga tagaattcgc tttaaaaaat ccaattacag cctttgcagt
     3661 aagccatttt tctgtaatga ttttatccaa catggcattt gcatctgcaa ataatttttt
     3721 ggcctcatct cctacaattt tatcttctag tattgtagga tatttaccgt gtaactccca
     3781 cacttgaaaa aatggcgtcc aatcaatata atttctcaat tcagctaaat cataatcgac
     3841 aaaagttctt gtaccgataa atgttggttt tggtggcgtg taatttgccc aatctatttg
     3901 agttttattc tttcttgcgt tttcaatcgt aagatattcc ttagtttcct tcttgctatt
     3961 atgtctgtct cgcatagcga catattccgc tctagtttct ttctgtaact tttcacggtc
     4021 ttgctcattc ataatttgcg tagcaatagg cacacttcgt gaggcatcta aaacgtggat
     4081 ggtaaatcca gaataatgat gctcaatttt tacagcagta tgtagttttg aagttgtagc
     4141 tccaccaatt aacaaaggca atttaaaatt tctacgttcc atttccttag caacatcgac
     4201 catttcatct aaagatggtg taatcaatcc actcactcca acaatatcta cattttgttt
     4261 aatagcttcg tctaataatt tttctgttgg caccattacg cctaaatcta ttacttcaaa
     4321 attgttacaa gccaatacaa cacccacaat atttttacca atatcatgta cgtctccttt
     4381 taccgttgcc aacaaaacct tacctaattt tttactttct ccactttgtg atgccataat
     4441 aaaaggctgt aaataagcta ccgatttttt catcaccctc gcagatttta caacttgcgg
     4501 taagaacatt ttgccacttc caaataaatc tccaacgata ttcataccat ccatcagtgg
     4561 accttcaatt acttctaatg gtttatcaaa tagctttcta cattcttcgg tatcttcatc
     4621 aatatattca accaagcctt tgactaatgc atattttaat cgctctttaa cgtcttggtt
     4681 acgccattct aaatcttcct cttttttctt gccaccacct tttacttttt cagcgtattc
     4741 taacaatctt tcagttgcat catcttttct gtttaataaa acatcttcta catattctaa
     4801 aagtgttttg tcaatactat cgtaaacttc tagcatggaa ggattaacaa tgcccatatt
     4861 cataccattt tggataccat gatataaaaa tgcggagtgc attgcttcac gaacggcatt
     4921 atttcctcta aatgaaaaag aaacgttaga aacaccacct gaaacatgag ccccttttaa
     4981 attttcacgt atccatttgg cagcattgaa gaaatctaaa gcattatttc tgtgttcatc
     5041 taatccagtt gcgactggaa aaatatttgg atcaaaaata atatcattcg gattaaaatt
     5101 aacttcatta actaaaatat cataactgcg tttgcaaatt tgaattcttc tatcataact
     5161 atcagcttgt ccatcttcat caaaagccat tacaacagtt gcggctccgt atttttttat
     5221 caattttgct tgatgaataa actcagcttc gccagatttt aaagaaatag aatttacgat
     5281 agatttgcct tgtacacatt ttaaaccagc ttcgataatt tcccattttg aagagtctat
     5341 cataattgga atacgagcaa tatctggttc agatgcaatt agattcaaga atgtggtcat
     5401 tgctttaaca ccatcaatca taccttcatc catattgaca tctaatactt gtgcaccgcc
     5461 acgaacttgg tctaaagcaa ctgccaaagc atcttcatag cgatcttctt ttaccattct
     5521 caaaaaaact tttgaacctg taacattggt acgctcacca atattgataa aatttgattc
     5581 ctttgttacg attagtggtt ctaagccact taattttaag atattacaat cagtttccat
     5641 ttatgttaca ctatattttg atggataata cttatcctat ttttatttaa catacacttt
     5701 ctgttagaat ttgtctaggc ttgtaggctt tagcagcctc tgcaaatgct ttaatgtgtt
     5761 gtggtgttgt tccacagcaa ccacctaaaa tattaattaa gcccatttct aaatattctt
     5821 tcacagcatc ggtcatttgt tcaggtgttt catcataacc accaaaagca ttaggcagtc
     5881 ctgcatttgg ataagcactt accataaaag gtgcagattt tgccaaaatt tgcaaatagg
     5941 gtcttaattc tcttgggcct aaagcacaat ttaatccgat agaaaataaa ttcacatgcg
     6001 acattgaaat taagaaagct tctgtagttt gtccacttaa agttctgcca cttgcatctg
     6061 taattgttcc cgatatcata ataggcaact cacgacccat ttcttcaaat aatgtttgta
     6121 ctgcaaataa agcagctttt gcatttaaag tatcaaaaat ggtttctatc aataaaatat
     6181 ctacacctcc atcaatcaat gcttttgctt gttgatgata agcagtaacc aactggtcga
     6241 aagtaattgc acgaaagcct ggattattta catctggaga aagtgaagca gttctattgg
     6301 ttggtcccat tgcaccagca acaaatcttg gaaaatcagt aaactcatta gcaacttctt
     6361 ttgctaattt cgcagattga aaattgatat cataaacagc atcttccaaa tgatagtctg
     6421 cttgtgcaat agttgttcca gaaaaagtat tggtttcaac aatatcagca cctgcttgaa
     6481 aatactggcg atgaatttct ttaataatat caggtcttgt aatggaaagt aaatcgttat
     6541 taccttttaa tggatgttga tgttctttaa atctatcgcc tctaaaatca gcttcttcca
     6601 atttgtaacg ctgaatcatt gttcccatcg caccatctaa gatgaggatt ttttgtttag
     6661 cgatttcgta taaattatta ctcataataa acttttaaaa tcatagatat aaattaaact
     6721 atgaaagtca ttttcaataa aaaatattac gaaataagga taaggtaaaa atattactta
     6781 ctcattaatc ctctaaattg aggtaggttt tggcaccgtt tttagaaagc tatttatttt
     6841 ctagccatta attttctaaa aggttgccaa gacttcagcg ggcctattcc ctccgtcttt
     6901 cgcaataagt tgaattaaaa aaagaactat gctacaaaga tacagtatag attcaaattt
     6961 ccaaattgtc ttgtggtaat aaaattagaa acaacttttg tttaaaatcg gcaaaacacg
     7021 aaccaatcct tgtaaattgt ttgcgaaaaa ctacaatagt gtcttttcta aattattaca
     7081 ttatctttac actatgcatt ttgtatttcc gacttttcta tatgcattaa tcgccctcag
     7141 cattcctatt attatccatt tattttattt tagaaaatac aaaaagttat attttcctga
     7201 tatccgtttt ctgaaaaaac ttcaagaaca gaaatccaat atcaaccaac tgaaacagcg
     7261 gttgatttta ttggcacgtt tggctgccat cttttttttg gttttagctt ttgctcaacc
     7321 cttttttggt aagaaagaga cgcttaatcc tgcaaatact tatgctgcgg tttatataga
     7381 caattcgcct tcaacacaat tgataaaaga tggagaaaca attttggata ttgaaaaaaa
     7441 tcaagcgaaa caagttgcaa atgcactcaa tccaaatgat aaaattgcat taattacaaa
     7501 tgatttatac aattcttacc aaagatggat gaacaagcaa gattttatta atgcggtaga
     7561 taacataaaa tcaagtaaac aacaaaaaag tttggaagag gtatttttaa aacaaaatgc
     7621 tttgttggaa agtgcgggct ctaatccaaa aaacagattt atattttctg attttcaaac
     7681 ttatattatt gataatttaa aaattgacac aaattttacg acctatttat tacctatcca
     7741 aactaaaggc gagaataatg tatttgttga tacctgttgg ttgggaagtc ctgttgtgca
     7801 attgaatacg aataataaat tatttataag gtttaaaaac tctggagaaa aaacggttac
     7861 caataagtct gtaaccttaa aaatcaacga acaagtaaaa tcggtaaaag atataaatat
     7921 cgcagctaat tcctattctg tagattcatt tactttttca attcaaaaaa gcggtgttca
     7981 aaatatagaa gttgttttta atgactatcc aattacttat gatgatgctt tttatgcgag
     8041 ttttgaggtt aaacaaaatg aacatatttt gagtattgaa gatgttgaaa gtacgaaaca
     8101 tatatctgca atatttttag ccgataatta ttttacatat accaaaaatg atgttcgaca
     8161 aatagattat tctaaattag catcgtataa ttttatagtt ttaaatcaga taaaggagat
     8221 tagttctgga ttgataaaca gcttaaaaat atatattcaa aatggtggtg ctttgtttgt
     8281 tattccagca aaagatatca atttagaaag ctataatgtc tttttcaata ccttaggaat
     8341 tggaactttt tcggcaatta aaacacaaaa atcagaagtc cgaaaattga atttgaatga
     8401 aaaaatttat caaggtgttt ttgctcaaat acccaaaaat attgatttgc ctaagattga
     8461 aaaattttat cctttaaatg ttaatgcaca aacgatgcat tatgatatta tgtcaagcac
     8521 tacaaacacg cctttacttt ctaaatttat agtggacaat ggtattgttt atatacaaag
     8581 tacaccttta gatattagtt tttcagacat tgggacgaaa gcagtttatg caccaatcgt
     8641 atataatatg gccatcctaa aaaattatgc cccaccactt ttttatacaa ttggaaaaga
     8701 tattttgatt aagattcctg cattacaaaa taatcatcaa gaacaagtat ccataactaa
     8761 tcaaacacaa gaatttattc cagaaagtag aatgcttgga aatactttat ctttaaatgt
     8821 caaacaaaat ttggaaagag atgggtttta taaaataaag attggaaatc atacaatttt
     8881 gggcaatatt gcattcaatt tcaatagaaa agagtctgat ttgaatttcg ccactattga
     8941 aaatttagaa agtcgttttg attttccaaa ttttaaaatc ttagaacaaa aaaattccaa
     9001 tatttttgca caagtaaaac atataaaaga cggaacagta ctttggaaaa tttttattgt
     9061 tttagcactt ttatgtttat tgatagaaat attattaatt cggtttttaa aataataata
     9121 cagtgaaaat acttattcta caagttacaa ttttagatga aggaagtcct tatcaccaac
     9181 aaaaagtgga cattttgctt gaaaatggta ttattaaaaa tatccaaagt aaaattgaaa
     9241 agacaaatga ttttgaaatc cataattttg aaggttgtta tctttcagtt ggtttttgtg
     9301 atttgattac tcaaattggc gaaccaggat ttgaatatcg tgatgatgtg gaaagcgttg
     9361 caaaatctgc tatttttgga ggatttacca cgatatgtgc tttgccaaat aatcacccaa
     9421 ttacgcaaaa taaatcagat gttgaattta tattgaatag agcaaaatcg acagcaattc
     9481 atatattacc ttttgctgct ttaaccaaag attttaatgg aaaatctccc acagaaatga
     9541 tggatttgaa taaagcaggt gcaattggtt ttacagatat tccacataac attaaagata
     9601 atggggtatt attgcgagct ttacaatata cccaacaatt tggtggtgtt gtttttgctt
     9661 tgccatttga taaaaattta gtgcatgatg gacaagtgaa tgaaagtatt gtttctgtga
     9721 aattaggttt tagaggtatt cctgaaatct cagaatatct tgcggtatat caaagtatcg
     9781 aaatattaaa atatgcaggt gggaaactcc acttgacagg aatttcttct gaagactcca
     9841 taaaactaat tgctcaagct aaaaaagatg ggttgaatat tacagcttct tgttttgtgc
     9901 atcatttgat ttctactgaa gaaagtgtag caacttataa ttctaatttt aaagtttttc
     9961 cacccttgaa gaatgacaaa acacgattgg cattaattga agcggtaaaa aataaaacta
    10021 tagatgtaat atcaacgcaa catacaccct tagattcaga agttaaaaat ttagaatttg
    10081 aatatgctga ttacggtatg attggaatgg aaactgcttt gggcttattg ctgaaataca
    10141 cagatattcc tttagaaata ttaatagata ctttatccta ttcacctaga aaaattataa
    10201 atcgtcaatt aacgatagaa aacgataaaa ttggagaatt taccatattt aatccttcag
    10261 aagaatggaa ttttgaagag caaaatatcc aatcaaaatc taaaaactca ccttatattg
    10321 gaaaggcatt aaaaggaaaa gtaaaggcta tttttaataa aggtgtttta actcaaatac
    10381 aataagctat gaaaattctg atggtttgtt tgggcaatat ttgtcgttct cctttagcag
    10441 aaggtatttt aaaagataaa tgtaaaaaga aaggattaaa ttgggaaata gatagtgcag
    10501 gaacaggaag atggcatgtc ggtagccaac cagataaacg gtctatcgaa gtcgcaaaaa
    10561 atcacggaat tgatattact gaccaaagtg ctagaagtgt aaatagtaat gattatagtt
    10621 attatgatat tatttatgct atggatacaa tgaatttaag agatttaaaa gcatgggctg
    10681 tagatgctaa tgaagaagga aaaatcagac taattttgaa tgaaatagat agttttccaa
    10741 ctgaaagtgt tccagacccc tattttaatg ataatggctt tgaggcggtt tttaatatgt
    10801 tggataaagc ttgtgatgca ataatcaaaa aatacggttg aggatagaca gaaatcttaa
    10861 taggcttgga ttaatacttc tgatggaatg tgtaattttt ctgtgagttt acgaatcatt
    10921 tccaaagata attttctctt tttgttcaaa atttcactag ctctgctttt taatccaatt
    10981 acttcggcta aatcattttg agaataattt aactgctcca ctctgaactt aattgcttca
    11041 attggatcag gtaattcaac aggaaagtgt tcattttcat aattttagag taatattccc
    11101 agaatttcca attcatcgcc ttcattagtt cctgacttgg catcaaaaat caattcaagt
    11161 ctttctaaag ctttaaaata atcttgctct gttttaatgg gttttaattt catcttttaa
    11221 attttattta catctatttt gtcatattct gcatgagttc cgataaatcg aatccacatc
    11281 atttgggaat taaaattaat tttcacgata aatcgataat tatttccctt tatgttaaag
    11341 caaactttat tttcatctaa aatacttgca cttagaaatt cattttttaa ttcattctaa
    11401 ttctgccaag cttctttgtt cgcttcttga tacaatgatt taagttgttg tacacaatca
    11461 gaatgttttt cccaaaagtc tcttaatatt ctttttgcta taactctcaa ttaattttct
    11521 ttagacaaaa ataaaaaatc atttcccaat ttgggaaagt ttatcatgac ttttttcata
    11581 tatttttaag ttattaatgt ataagcctat tttcagggca agatattagg ttcatcatga
    11641 gtacttaatg aatcaatact gagtctttgc tatcagggga aacccttgct attaaatccc
    11701 atattttccc cgataaaggc tacgtatttt ctacccaatt ttgcttcatc aacttataac
    11761 aataagaaaa tgaaagcaaa cttattaaga aatatcagct atgcacttgt gctgttgctt
    11821 actttttcat cttgctccaa agattatgaa agaataaaag acccgattga agaagaaacc
    11881 cctgttacaa tttacatcag cggttattcc aatgaaattg ccaatatggg cgaagtcgta
    11941 tggaaaaatg gaaaaccgat tcaggtgtac aaaacaggtg ttttaccttc tggatatgtg
    12001 cgatatggtg gaattattta cgattttgat atcgaaaacg ataatgtgta tgccgtttgg
    12061 aaactgagcc acaaaaaact atcagcctat ttcccttctg cacatattta tttgtggaaa
    12121 aataatgaaa acacgcttct tctacaaaac gattacaaca tatatcctaa agcagtggac
    12181 aagcatggag acgacctcta tttcgcaggc gattatacca ctccgcaatc ctacccaagt
    12241 atctggaaaa acggcaacag agaacaatta cccggaggta tcggctcagt ttctgactta
    12301 ctcgttacta atacagatgt tttagccgtt ggttacgtag ttactggctc acaatatgct
    12361 gccgcttttt ggaaaaacgg tgtactcact aaattgaacg aaggttttag ctataccgca
    12421 aattcaatag cacaagcagg atacgatacc tacatagctg gtagcggtaa agctggagta
    12481 aacgacccac ttttggcatt gatttggaaa aacggcatac aacaaacact gttacttcca
    12541 gcggggcaaa ttggtgcatc atcaaattcc gtagctattg gaggaaacaa agtgtatgtt
    12601 gtaggttatt cttacacagc agacaatgac agtgaaaaag ccgtactgtg gatagacggc
    12661 agccctttga ttttgtcctc aaccaaaagc atagcatatt ccgtagtggt aaaaggcaat
    12721 accatatatg cttgtggata tgaaaagtcc agcactaaac agagagcggt aatgtggaaa
    12781 ataaaggatg gcaacataga aaccactaag ctaagtaatg gtacagaaga tgctgttgcc
    12841 acttgcatta aagttaaata acaaaatatc aggtttaaat aaaaaagtat gaaaacaaaa
    12901 ataatactca taggacttgc gttagccatt gggttttctg caaacgcaca gatcatcaat
    12961 cccaaagaaa ctactaaaca aaaagtagaa gacagaacaa atcgaaaagt tgacgaagca
    13021 attgataaag gtttggataa aatagaagat ggtattagca atatcttcaa gaaaaaggat
    13081 aaaaaacaga aagataacag caatgatcat tctcaaacag ataataccaa taattctgac
    13141 actaatcaaa atactactca aaacggaaat actgacttct cgaagtataa aaactttgat
    13201 tttattcctg gtaaaaatgt tatcttcttt gataatttca gcgatggttc taaggctcgc
    13261 tggaaagcct acgatgaaaa tcaattaagt atttctgcta atcaggaaag caactggctt
    13321 gaaatggggg gtacgagatt tgccccgata aatgtaggtg tactacccga agatgttacc
    13381 atagaaatgg atgcttatgt gcgtaatggc aagcagcctt gcggtacttt ggaaattagt
    13441 tttgtagagc aatcggagaa agaccgcctc gaagaccctt cgttggataa ttcaagcact
    13501 atttcaataa gcccgattac cgaaacgcca aaaaatgcaa ctatctctta tgagaagaaa
    13561 aaaaataatg aagtaatcgg ctatactatg ggttttggat ttaaaacttg gcaacctgaa
    13621 ttgggtaata cgcatgccca cattagcatt gcacgtaaag gaaatagcgt gtctgtgtat
    13681 ataaataatg aaaagctatt agacaatatt gacttgctca acagcgatat gaaatatatg
    13741 cttgctatga atttgcagaa ttattttgta gaagaaacaa ggatgtacat caccaatgta
    13801 cgtattgcaa atggtacatc caacgctgtt agtgaagtaa aattaaaagg taaattcgta
    13861 accaatacca tttattttga tgttaattct tcacacatca agcctgaaag ctgggttact
    13921 ttgaagcaaa ccgcagatgc tatcaaagcc aattcaggga acttcctgat tgtagggcac
    13981 accgacagcg atggtgcaga tgatgtcaac ctcaaattat cacaaagccg tgctgcttcg
    14041 gtaaaaaatg ctttggtaaa agaatttggg atagatgctt ccagattaat tacagatggt
    14101 aaaggagaaa accagccagt aagctccaac aataccgcat taggaaaagc acagaacaga
    14161 agggtggaat ttattatgga ataaaataga gatgatgaac aacttattaa tattagttct
    14221 tgctttaatt ttctttgctt cttgcagtgg taatattgaa aacgtgagtg tccccgatga
    14281 ttttagttcc gaaaaaataa tttctaaaca gaatcggcta cagcaaatcc ttaccaaaga
    14341 aatggttgcc aaagccgtaa atgttccgat agaaaaaata gaaatatcgg tagagaacag
    14401 cattaatcga agcggacaat atactgcttt gtttagttgg actacgggag ataaaaaaag
    14461 ggtgggcaat cacgagattg acgaatacca tagcatcagc attggtttta taaagcaaat
    14521 gaacatagat gattttgaaa aatactatgg tacgaacgag ggtttgcaaa tggaggtgga
    14581 caatatgtca aagcaggagt attttaacaa agaaataggt acagccgaag caaagtatat
    14641 ttctgaatat gctcgtactc gtaaaatgca aaagctcgat aatgtggcta cagtggcatt
    14701 ttgggagacc ccaatgaatg cactacacgt actcgcaaat gatgctacat ttaccgttac
    14761 agtcaatttt ggcgatgatg aagtattggc aaagaaaaaa tctgtagagc tgactaatgc
    14821 tattctgaac aattaaaatc tttctgttat gcgaatatat tttttaataa ttggaatgct
    14881 tatttcggtc tcgatttttg ccgaaaaagc aatcatagcc aactgttcag agggtggcgg
    14941 atgtacattt gagctttcgg acattgaaag tgatttgctc gacaatgatt ttggcagaaa
    15001 tgttcagccc gatgacatta tcgtattagg gaaaaagaaa aacggcgagg ttattactta
    15061 tttggacaaa aaatcaaggg ctgccataga cagagactgg ggtggtgacg gttacacaca
    15121 gttgggacta tttaatccgc agatacagcc gctgaatgga atgtggaatg tagtatatgg
    15181 cagttctaca ggcaacgatt gttacggtat aggaaatata ggctcgtttt ttcgcaaaaa
    15241 gataacacca ggaacagcag gcaacggcaa catcaaattc cagtttccgt tcaatccgtc
    15301 acagcttttt ccagcaaacg atatgcgatg ggtaaagaca ggatataaca cttacaaagg
    15361 tttgctggac tttaacagca atagtatggg tggtatgaaa atgtactacc acgttaccat
    15421 atttagcaac aaaaaaatcg aaaccattta taccatagaa ataaaagtgc ctaccaagcc
    15481 gctctgtgtg gggaaagttc cggtaactta tactttatta gatgccgaag aaaccgaatt
    15541 cccagaagac gtagagtctg atgatgacct gctgccagtt aatccaaaag gaaaaaaaca
    15601 agaagatgac ctaatgccta taaacccaaa aggcaatgaa aatgatttgc ttcctgttaa
    15661 tcccaaaaag gaagacctgc ttcctgtgga gccagaaaag aaaaacaaaa caaaagtaga
    15721 acgtttggag gataagccga aagttccccg attagaaaac taaatatcaa taatatggaa
    15781 acaaatacac agcaaagcat aactgagggc aaaagcactg ccattattgc ttatattaca
    15841 gtcattgggc tgattattgc ctttgtgcaa aacagcgaaa agaaaaacac ctttgcaaat
    15901 tttcatatcc ggcaaagctt agggcttatg tgtacaggat ttgcattggg gcttgtaaac
    15961 atcattccta ttttgggctg gatagtttcc gtatttggaa tgattgtact tcttattctt
    16021 tggataagtg gattactcaa tgcattgaac ggaaaagaaa gccctgtacc tatattgggc
    16081 aacctataca acaaatggtt ttcgggtatt caataattta ctaataatta aattcataaa
    16141 aatgaaacag atacaatcta tagtaacatt tgctgcaatt tgcattactc tttattcctg
    16201 cggaaatggt gaaaataaaa gccaaaatgg caatgacgtc aatatagatg ccgtcatcaa
    16261 cagcaaatcc aaagactcca atgcaaaagg cgacaataaa tgccttttgg agtatcagaa
    16321 taaatacgat gccctgcttt cagaaaatga tgtaatcaag gcgaccggat tttcaaaaga
    16381 aaaaatggaa actcaataca ataaggtttt aaagaacgct gaacaccacg aatttctgta
    16441 caaattcaaa aatggcaggg taggcaaagt acgaggtttg gaccgggaaa tggaacagcc
    16501 cgatgtagta agcatccgct ccatcaaatc gatgtcactt accaccttta accaaagcta
    16561 ccaagccatt accgatgaac agatggagac agcaaacgat gtcattgatg agattgccga
    16621 aggcaattca ggcgatgcgg atgcagataa agctttgcaa aaggcaaaag agcagaatgt
    16681 aagtaaagac caaatcaaga aaacaggtgg aattttgaca ggagctttca aagaggtttc
    16741 cgaaggttac agagctgtag aaggattagg cgatgccgca acctggaatg tggttaccaa
    16801 tgagctgatt gttttgcaga atggtgtaaa gtttgaaatc ttggcggata tcagcaacga
    16861 taacgaacaa aataaatccg tagccataaa gctggcggaa atcgtattga acaagtgtaa
    16921 ataaaccaag acatatttca atgagattaa ccacttagat acagtttaca ggcatttcag
    16981 acggttcgta caattttaac atagcaggtt atcaatcata tcaaaacaca agatgaagaa
    17041 taggtttatc aaaatatttt ttataatttt tttgcttttt ccgttcttgg tttttgcaaa
    17101 aaaaaagagt aaggtaacaa tctctaaaaa tttaaatttc tccgaagtgt gggtgtggga
    17161 atacaccgat agtaaaggca aaaaaatcga aatggcgata taccgtgagc cgatattaaa
    17221 ttattggctg cttacaactg aagcctactg gcaaaccgat gaaatgagcc tgtggtttat
    17281 attaaaccca aatggtgaag tcctacaagc atatcaagac ggagaaagta atagctcgaa
    17341 aaggctaatg aagcatagtt tatatcctga taaaaataca gtattgccaa acaattggaa
    17401 ggcaacagga attacaaagt cattcggtga tgtcagctta ggatttccaa agtttatagg
    17461 tgaggaatat aatgtcagct acgataaaac caatgagcag tctgtgtttt ttttgacacc
    17521 tacaaaagca gatttttcta cattatatct tttcaatggt ttggatatag atgcaaagtt
    17581 gcccattcgt ttccccaaag atattcctag taattatgtt gtattaagtg aagaaacaaa
    17641 gttttcaaac gtttcagaat tgtatcgttt taaatatata tcgcataccg attaccatat
    17701 taatatttca gaatataatt taaaataaaa tagatagcga tagaaatcct cttgtgtttc
    17761 gatataattt aagtcaaata ttattgaaat aaaaacagga taactacaaa ttgaataaaa
    17821 gataataact aattttatat cgtgtaaaaa ccttaaaatt agttagatga aaattttact
    17881 tgctgacgac cactttttag tactagaagg actagaggta cttttatcta cttttgattt
    17941 tgtaaaaagc acccaaagtg taataaacta tatggagatg aaagaggttt taaagcacga
    18001 aacgtttgat gtattactgc ttgatattca ttttggaaaa cacgatggca gagtaatcat
    18061 aaccgaaatt aaaaaaatga tgcccgctat aaaaattatt gcactcacaa gtcattcaga
    18121 tgccaccaca gtaaaatctt cttttaatgc aggttttgac ggctatctgt tgaaaataga
    18181 taatagagaa gaaattgaaa aagcattgaa agctgtatcg agcaatgaaa aatattttag
    18241 ttcaaaaact cagcaagtct tttttgaaaa tcaggtatcc aagaacaaag tagcacttac
    18301 cgaacgtgaa aaagaaattt tgcaattgat agtcgaagaa aaaacgacaa aagagattgc
    18361 agaacagctt tatctttcag aaaaaactat agaaacccat cgagggaatt taatgataaa
    18421 gctcgatgtg aaaaacattg ccggtatggt aaaaaaagca attattcaag gcttagttaa
    18481 catttaatca aatccaaata tgcgaatata cgttctgtta tttttgttgg ctactttgat
    18541 gcaatcttgc agcaattctg gcacaaatca gaccactaaa aatgcggtta gtagcgaaga
    18601 tagtttgcag ataatgaatg cttttgtccc tccgtttatc aatgccgaca gtatctataa
    18661 ctcattgcaa actgatttta gtaaaaaagc agcaactatt gatttcctca atctaaatta
    18721 tggcaaagtt ttgataatga gtggcagatt agacaaagca gatacattga ttacatcggc
    18781 aataacccgc acaggctttg atacgacaag ccttgctcat gcacggtatg ccaatctgca
    18841 agcggctatt gaagcctaca agcaaaatca ggaaggagct attggctatt acaaaaaagc
    18901 cattcagcta tttgaaaagc ataacgatac caaaagtgcc gcatctataa acttcaatat
    18961 tgctaatatt tttctaagcc gtcttaacta ccctatggct taccagtaca gtgctgaagc
    19021 tgtaaaagga tttaaagcag caaacgacac gctctattat ccaagtgcat tggcagtaaa
    19081 tgccgtatcg gcaattgttt taggcaaaaa agaagaagct aaaaccacag cagagcaagc
    19141 ccgagaaatc agcgagcgat acaaaaatgc tttaggtata gtaatgtcta gctatgcact
    19201 agccgaaatt gcgatgtacg ataaaaagta tgacacagca atagcacaat ttcaaaaagt
    19261 aataccattg gcacaacaat tacagcagat aacggtaact tcagccgcct acacatcact
    19321 ggtaaaagcg tatttagaaa gtgaaaatta cgatttggca atcacagagg ggaaaaaggc
    19381 gattgagttt tccaaaaaat tcgggtacga agacgtgagc tattcactca accgttatat
    19441 ctctcaggct tatcagcaaa aaggcgatga acattcggct ctacaatata tgcgtatggc
    19501 tgatgagcat tttcgcgacg agttggtatc aaacgacagg cgagtgatgg gagagctttt
    19561 agtacaatat caaaccgaaa aaaaagaaag gcaaatagct gagcaaacgc tggaaattca
    19621 aaaccatcgg tcaaattttc tatatgctat ttttggtggg gtgttgctgg tcttggtttt
    19681 agggggattt tttatttata acaggaaagc ccaaaaacta aaactaaaac gactgcaaca
    19741 ggaaaaggaa aatgccattc taaattcttt tattttaggc gaagaaaggg aacgaaaacg
    19801 tatttctcaa gatttgcacg atggtgtagc tgcgatgatt ggagctgcta aaatgagtct
    19861 tgatgccata ccacaccttc cagaagataa acggctggag caactctcca aagtaaaagg
    19921 tattttagaa aatacacatg cggatgtacg gcatattgca cacaatctgc taccaacggt
    19981 attggaagta gaaggcttaa taaaagctac cacccatttt gcttttgaaa tcaatcaaac
    20041 caaattgata gacatttcgg taaaggatta caacagcaat gcaaaagacc tttctacaca
    20101 attacagctg atgcttttcc gggtcattca ggaattagta aataatatcg tcaaacattc
    20161 gcaagcacaa aaagccgaaa taatattcag caacagccca aatggattac agattgaaat
    20221 aacagatgac ggtataggct atgaaggtat caacaattcc ggaaatcaag gattatacag
    20281 cattacacag cgtttaaaat caataggtgg taattttaaa atcacaaaag gaagttctgg
    20341 tggtacccaa gcgaaagtag aaattgttgt ttaaaaaggc gttagggtaa aaaatacaaa
    20401 aagaataagg ttacattgta tcatactaaa atactttaca agttttcatt tatacttaca
    20461 tttcatctat tgttgtataa acctattttc aaggcaagac atcttgcaaa tcgcagaaat
    20521 taatggagtt gcgttttaaa gaaagtcatc attcgttcaa atgccttttt cccttgactg
    20581 ttattgaaaa ataaatgtgg aatgccgtgt ggtaaagcat aatgatacgt ttctaattta
    20641 tcaccta
//
LOCUS       DAOQCW010000034        20230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_960_length_20230_cov_5.051846, whole genome shotgun sequence.
ACCESSION   DAOQCW010000034 DAOQCW010000000
VERSION     DAOQCW010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 20230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20230
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_960_length_20230_cov_5.051846"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="PLS16_04565"
     CDS             <1..434
                     /locus_tag="PLS16_04565"
                     /inference="COORDINATES: protein motif:HMM:NF021623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNJ60384.1"
                     /translation="TALFGHALYKHVDVTREAIPEDLAELFQWHAAEEIEHKHVAFDV
                     LDLVDKDAYTKKVVFMPLATAILYFYLSVGTIMLMYQDRKHLSLLKLPKQFYEFTTGL
                     FAELHGEMVGEFLKFFKKDFHPSDIDDYYLAEEFFADKAYA"
     gene            620..1933
                     /locus_tag="PLS16_04570"
     CDS             620..1933
                     /locus_tag="PLS16_04570"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF006110.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HNJ60385.1"
                     /translation="MSDFLQNPMVKQLAKALNLPLPIPALLNRNNNAYADTEWQSKKS
                     ALVITSNEDQITPKILSDLKHFINEGTLYQDKIDTVVLSALGIENIDMSVEFFTKAQL
                     AVQKLNKNGRVVVLSKTGDDIEQQTIQKALEGFSRALSKELGKNGTTVNHLTITDAPT
                     RNVARAILFFLSDKSSFITGQVVQLNNNFSSELNSVSKSLNGKVAIVTGAARGIGASA
                     ATFIAREGAKVIIVDVPQAKEDAETLAKEIGGDVLLMDITKEDAVKTIQDYVLKNYTQ
                     LDILVNNAGITRDKTLTKMNIAQWTSIMQVNLKAAMNLTNAFIQNGFSKNPKIVGLAS
                     ISGIAGNVGQTNYSASKAGMIAYMNELSKKGFMANAVAPGFIETKMTENLPIFVKEGG
                     RRLSTLKQGGQPQDVAELITFLSTPLSDGISGQVVRVCGGSMIGA"
     gene            2088..3392
                     /locus_tag="PLS16_04575"
     CDS             2088..3392
                     /locus_tag="PLS16_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60386.1"
                     /translation="MNTKSILTLVVAFLFCMPQILSAADDIDKNNEKIEKLEAEIKSL
                     KAENQNLLNASKSDGSGAKLKIRNKVLRPAGKTWYMSASAGYGIPFLLANKKSPLKEI
                     GEGDWQQSPGALSRKPLFGTNGGGFALNLGWGHMFNKYIGIDVLHTLAWHPESLDARI
                     NTKSTSFLGTSSYYATQKTATMAIYVSPHLVMHWDNNKRFGITGKIGMVAPIFGNAIS
                     RAHIDDNSGRLLQTLNGIPPVLGIPFIHMKLDAVAKTKFHPTLGISSSIAFDVKLAKN
                     IWMFAEARVQAYTINLISTKMFEFKMETSLKLGDLEISTKVADDLIKQISGNLLSLSS
                     LTGGLPLNIANASEAPRFLTERRYVDEITENSNTARYGTKSMIGGPLNFTGLEAVDLN
                     KPMDEPGQRFNTSTLYFNVGLRVDFDLWDKKRSEKRKEKAAL"
     gene            3633..5378
                     /locus_tag="PLS16_04580"
     CDS             3633..5378
                     /locus_tag="PLS16_04580"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HNJ60387.1"
                     /translation="MKEVPENFTFDISFTVENGNLDKFTPPNFNGFDVYGPSQSQNIS
                     IINGNVSKSFSYTYTLQPKKQGSFTINPANAVINGKTFSTQPIAIKVIAPQQKQTQNR
                     RRQQNYDPFEDFFGGAESQRPSEAEIKKELNEGIFVKVTPNKTTLYEGDQVTLSYKLY
                     FRLRYDGLNATKMPTYNGFLSEDFDIPIDQQKEPEIEVYNGKKYYVQEFKRVALFPQK
                     SGKTTIDPMSFSCVVGVEIPNNFNPFFSDIEPYEYNFNSNAVNLEVLPLPSPQPKNFS
                     GAVGKFNFQASYDKTQVKVGEPIKLKIAYNGTGNLKLIAAPKLTFPEEFEAYEPKLNE
                     NYATKGSIVSGTKSFEYILIPQDGGTFKLPEYEFGYFDLDKKAYTTFKLPETEITVSG
                     KAKLSENIVNFMKREKKDKASTSIKGNIEQLSDNSILYNSTIFWALSAAPFAIFLIGF
                     LLRKKEYDAPELILLRKKKAHTMALNRMKTAKKLMTNNNEKGFYDEVIRALLQYVSDK
                     LHIPMADLSKENISLKLSNRNISVEKTEQLKEMLTACEMSLFASINKSDSMKTTFQNA
                     IDWIINIDEDLSKIS"
     gene            5380..>5609
                     /locus_tag="PLS16_04585"
     CDS             5380..>5609
                     /locus_tag="PLS16_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60388.1"
                     /translation="MNRLKILIFVLFVGIFYFGFSNNINQQINLSNKYFKEKQYEKAA
                     NILTGLLDQKIYNSTVYYNLGNCFYQLKEYPKA"
     assembly_gap    5610..5709
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5710..5961
                     /locus_tag="PLS16_04590"
     CDS             <5710..5961
                     /locus_tag="PLS16_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60389.1"
                     /translation="SFVIALMLFFICYKNYIYQTKQTHAIVMQSGDTYPSPNKHKKYK
                     NGLEEGSKIKLLDNDGGFYKVQTSNDNVVWILKSQVAEF"
     gene            complement(5963..6790)
                     /locus_tag="PLS16_04595"
     CDS             complement(5963..6790)
                     /locus_tag="PLS16_04595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNJ60390.1"
                     /translation="MRKVIVLIIAFFSILATFAKPDNFEGIASYYGSYFHGRKTASGE
                     LYNQYKLTAAHKTLPLGTVVKVTNLSNSKSVFVKINDRGPFVKGRVIDVSTKAADLLG
                     FKNKGTTKVSVEVVEPDEVPADLSISSEALAEYKKEIKQEDTSWMNIPIVEDNEQKPA
                     PAPMQAPINNELNVNNEGMLNRKDLDLTKLSILEKVTAGYFGIDLGNFFDLVELKELI
                     KLLLAQYQQPIYFDQIASETNQPTYKLFMGNYKNKAYADALQLKLAADFKNCAVVQW"
     assembly_gap    6961..7060
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7061..7378)
                     /locus_tag="PLS16_04600"
     CDS             complement(<7061..7378)
                     /locus_tag="PLS16_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60391.1"
                     /translation="MEIQKEYLTITKTAKIVSYGKYTEATKVVWLVAHGYGFLAENFI
                     KRFKDLDEQEHFVIVPEALNRFYINGLSGKVGANWMTTEERESEIDDYIKYLDDVYVK
                     FNLH"
     gene            7544..8518
                     /locus_tag="PLS16_04605"
     CDS             7544..8518
                     /locus_tag="PLS16_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNJ60392.1"
                     /translation="MSNSVDNIKLPIEDELKIFEKKFRDVMRSHVALLDKVNYYIYQR
                     KGKQVRPMFVFLAAKIVGEVNENTYVGASLIELLHTATLVHDDVVDDAYERRGFFSVN
                     ALWKNKIAVLVGDYFLSKGLLYALETKNYNLLHITSDAVKRMSEGELLQMEKARKLDI
                     EEEIYFEIIKNKTASLIAAACKVGAASVTQDEALINKMHKIGELIGIAFQIKDDLFDY
                     GEADIGKPRGIDIKERKMTLPLIYTLQHSDKATKKWIINTVKNHNEDKQKVNELIKYI
                     INNGGIEYTEQKMLEYKNLAYQAINELPDSDSKQAFIQLIEYITNRNY"
     gene            8524..9540
                     /locus_tag="PLS16_04610"
     CDS             8524..9540
                     /locus_tag="PLS16_04610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNJ60393.1"
                     /translation="MNNSTNKLIIQTYTLIIGILLLLTKLMAYILSSSNAILTDALES
                     VINILAGAFGLYSLFIASKPTDDDHPYGHGKIELISANIEGTLIFTAGLVMVLKSIYN
                     LMFPVELKYLDFGIVLIAGTGIVNYIMGWYSKKAGTKYSSATLISSGVHLKTDAYSSF
                     AIIAGLIIIYITGMSWLDNILSIMAGLFIIYSGFKIIQSSADGIMDRADFILIDKTVN
                     ALNKNRKKEWIDIHNLRIVKYGENIHIDCHTTVPWYWNTQVMHDELKHVEDAIKEAIP
                     TRLETFIHADPCMPKSCSICMIEDCGVRQKPFEEKVEWTITNITKNEKHNSTNIISNN
                     NNTQ"
     gene            9542..10876
                     /gene="purD"
                     /locus_tag="PLS16_04615"
     CDS             9542..10876
                     /gene="purD"
                     /locus_tag="PLS16_04615"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNJ60394.1"
                     /translation="MNILLLGSGGREHAFAQKINQSLLCSQLYVSPGNAGTAKMAFNV
                     SLPDFESIKKFCIEQQIKLVVVGPEQLLVDGIYDYFVEDAILKNIPIIGPSKKGAMLE
                     GSKAFSKQFLINHNIPTAQYKEITAANIEEGFTFLETLQAPYVLKANGLAAGKGVLIC
                     QDLAEAKSELATMIDGKFGAASSTVIIEEFMHGIEFSVFVLTDGKDYKILPVAKDYKR
                     IGEGDTGLNTGGMGAISPPSFVDDAIMSVVEETIIKPTILGFQKDEITYKGFVYIGLM
                     LTPGNIPKVVEYNCRMGDPETQAVFPRINSDIVEVFMALANGTLKDVEFSISNKVCAT
                     IILASKGYPEEFEKGKVITHIEDVKDAYVFHAGTTLAAYLPRPCCEDESEAIKNGIVN
                     KDAHNIISTGGRVLAVSALGKTIDEAVAIANKNAEIIQFENKYYRKDIGFDM"
     gene            complement(10959..12032)
                     /locus_tag="PLS16_04620"
     CDS             complement(10959..12032)
                     /locus_tag="PLS16_04620"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60395.1"
                     /translation="MKNILNCLLVFTIALIPFSVQADCLSGDCKNGKGKAIYYDTENT
                     SFTYEGNFVNGQWQGEGLIIIPNKSTYKGTFKNGEVTGKGEIVYADGNKYVGEVLKFT
                     KEGEGLFTYKNGQTFKGIFKGDGVWNGEGYVYFSDKSYYKGTIQEGKQQGKGFFRFED
                     GTTYEGDFSNGIISGQGTVNYHDGSKYVGAVSNSVRNGYGKLYDKYGKLLFEGKWVND
                     NAQQNTLQKENEQYKQVFNRCYNGFERLTYNGQTGNADIHLCCTVSDDLSLTGTYTFT
                     ININGKNYIYKSEIYGSINKSKNSFTFYDNVVLRKDDVPNGHWTSLDSGVTLTIYNDE
                     NHKGYYVLKGKSVNGNPIELSDW"
     gene            12155..12571
                     /locus_tag="PLS16_04625"
     CDS             12155..12571
                     /locus_tag="PLS16_04625"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNJ60396.1"
                     /translation="MDTDIYFMQKALDLAKQAYKNNEVPVGAIICIENTIIGKGYNQV
                     ELLQDPTAHAEILAITAACSYLGSKYLTQATLYVTLEPCQMCKGAIAHAQIPRVVYAA
                     TDTARKTDTSKIYEGGLLVVEATELMKQFFREKRMF"
     gene            complement(12568..13335)
                     /locus_tag="PLS16_04630"
     CDS             complement(12568..13335)
                     /locus_tag="PLS16_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60397.1"
                     /translation="MEENKTIQSILKEGYQLEFINYIKSGFGLYKASFFSYITFTLLA
                     MMANEQVLRIPKFGGFLFFLLAPLQFGYFIFAEKVYKKQESNFKYFFDGYQRIVPIAL
                     LYFTNIAIFTFILILFFMPIANSQDIQDIKNFVIANPNLLNETKFPELPVISMKTRIV
                     FFVMMMTFLYTAILLSLASYLLWFQQMPVIKSLVYSVLIVKKKFSRWILFASVLLMLN
                     VLGLMAFGIGILITMPVSLLAFYVAYENVVGSETSFT"
     gene            complement(13424..13636)
                     /locus_tag="PLS16_04635"
     CDS             complement(13424..13636)
                     /locus_tag="PLS16_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HNJ60398.1"
                     /translation="MSFFKSNFITIIGVIVGAIAGYLYWLNIGCDSGTCAITSKPINS
                     TLYGALMGGLLFSIFKAEEKQNSIKK"
     gene            complement(13637..14218)
                     /locus_tag="PLS16_04640"
     CDS             complement(13637..14218)
                     /locus_tag="PLS16_04640"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNJ60399.1"
                     /translation="MRKILVFGIISLVVFSCQTNNKTLTLSEQQDYLSKGDSIATQAQ
                     QVLMKNVSAAIQEKGVAGAVDFCNINAMPLTDSVSNLNAVSIQRISEKNRNPNNAIVS
                     ELDKSALEQIKKMMADATIADKHLILQESGNVYYYKPIPLGMPTCLSCHGNTTTDIAP
                     ETLQVIQSKYPTDKATGYQLGELRGLWKIKMNS"
     gene            complement(14296..14613)
                     /locus_tag="PLS16_04645"
     CDS             complement(14296..14613)
                     /locus_tag="PLS16_04645"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNJ60400.1"
                     /translation="MFGFIKNMMGSGNEQLATVIKEGAFLVDVRTPGEFAGGSVKGAV
                     NIPLDKLQSQLSKFKGKTNIVVFCLSGGRSGQAKAFLDKNGIANVINGGPWTNVRQIV
                     NSL"
     gene            complement(14702..15556)
                     /gene="folP"
                     /locus_tag="PLS16_04650"
     CDS             complement(14702..15556)
                     /gene="folP"
                     /locus_tag="PLS16_04650"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNJ60401.1"
                     /translation="MIDIKENNSFVLNCGGTLLNFAEPKVMGILNITPDSFYDGGRYN
                     SETAILKKVEKMLSDGADIIDIGAVSTKPSAEDVTPEIEKERLLPILKLIKKTFSESI
                     VSVDTFRSSIAEMAINEGAVFINDIYAGVVDKNIFEIAARNNIPIAMMHIQGTPKTMQ
                     LNPTYSNVITEVFDYFVERIDVAKSYGVKDILVDVGFGFGKTLEHNYTILKQLDFFKL
                     LGYPLLVGVSRKSMIYKLLENTPQEALNGTTVLHTIALQNGANILRVHDVKEAKECIK
                     IVEMLKKN"
     gene            15654..16214
                     /locus_tag="PLS16_04655"
     CDS             15654..16214
                     /locus_tag="PLS16_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HNJ60402.1"
                     /translation="MKSELGKTIQQYDAAFSKCRNIFITKVRDYGTSWRILRLSSVVD
                     QLYIKAKRIRTIDEKGEQKVDDSVEDEFIGCLNYSIIALIQMELKTENNETIELEFPI
                     VEKLYDKYYQQIKELMLAKNHDYGEAWRDMRITSFTDMILTRLHRIKQMEANQGQSEI
                     SEGIDANLKDIANYAIFALIQLSENK"
     gene            16227..17375
                     /locus_tag="PLS16_04660"
     CDS             16227..17375
                     /locus_tag="PLS16_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNJ60403.1"
                     /translation="MKIITQVCRFLVGALFIFSGVVKAIDPAGTAIKMEEYFEVFAGY
                     LPKLNFLWHFLAHQALSVSVFMIVLEILLGVTLILGIWKKFTLWSLALIIIFFTFLTW
                     FSAKTGKVTDCGCFGDFIKLEPKQSYHKDLVLSVLILIIIIGARNIQLLFSRKLSTII
                     FGILTIATLLFTFRNIYYEPIVDFRPYTVGTSIPDCLQLPPDAKKPITEMVFIYKNKE
                     TGKEERFNNTFPQDIDNWTFVDREDKIIQKGDEPKCKDFAIRDANGNDNSAGFFEEEE
                     YVFLIICPNIKKASDRGFDKLLPVCKAAEKDHRYVFMLTGSVLEDVEKYKIEHKIPYE
                     TYSTDETPLKTIMRSNPGLLILKKGVIVAKFHYNELPDYQTIKKEYLK"
     gene            17377..18438
                     /locus_tag="PLS16_04665"
     CDS             17377..18438
                     /locus_tag="PLS16_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ60404.1"
                     /translation="MGKFILNKLAYGILVLIGVVFIVFALFNLLPVDPARLTLGQRAD
                     VASVEAINKELGLDKPFLVKLKLYLNDISPISVYENTPENQTKYHYTKIISLGESALV
                     IKKPYLRRSYQTKELVSEKLSKALPKTIILATFSIIFASIIGIFFGVLAAIRQHSWFD
                     TIAMSISNLGISVPSYFSAIILAYIFAILLHDITGLEQVGDITTIDDWGDEVLTLKNL
                     ILPVLALGSRPIGIIFQLTRSAMLDVLSQDYIRTAKAKGLSTKIVLFKHALRNAMNPV
                     VTTVSGWFAGLLAGAFFVEIIFDIKGLGYIAVNSLLNFDFPVTMGCVLFTAIVFIVVN
                     FIVDILYGILDPRIRIGNK"
     gene            18451..18948
                     /locus_tag="PLS16_04670"
     CDS             18451..18948
                     /locus_tag="PLS16_04670"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNJ60405.1"
                     /translation="MLIFLVGFMGCGKSYTGRKIAPILGYHYVDMDKVIEAQESMSIK
                     EIFEQKGEAYFRNLEHQYLLDLDTDQNMVISTGGGVPCFFNNMEIMNTKGITIYLNRS
                     KSVVIPRLLQGAHKRPLIQNMDEETLNTFYVTKLAERAPFYEQAQLHVGDANVEEILK
                     LLAIN"
     gene            19300..19464
                     /locus_tag="PLS16_04675"
     CDS             19300..19464
                     /locus_tag="PLS16_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60406.1"
                     /translation="MRNRAIFGKAVWLSFAKAKEKQRHPQAKASCFTFAFAQTHNDIT
                     TIIFNINQDI"
     gene            19481..19987
                     /locus_tag="PLS16_04680"
     CDS             19481..19987
                     /locus_tag="PLS16_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60407.1"
                     /translation="MEQAIIIIAAMTLLGVIALIFLYLTTEKKKKVAREAELTELKEL
                     LKTEVNNLLIKVQAKMQETTNQETEMFEALAKEVRTAQDQANAKINSVFEELQSENTT
                     LRKDVGEKIADIKSSFKEYSEKVKDNLNKYSNDNAEFKKGTELLKEQIQRELQNILKE
                     IKSPLDID"
BASE COUNT         7227 a         3081 c         3056 g         6666 t
ORIGIN      
        1 ttactgcttt atttggtcat gctctttata agcatgttga tgttacacgg gaagcaattc
       61 cggaggactt ggcagaatta tttcaatggc atgctgccga agaaattgag cacaaacacg
      121 tagctttcga tgttttggat ttggtagata aagatgctta tactaaaaaa gtagttttta
      181 tgccgctcgc aacagctata ttgtattttt atttaagtgt aggaacaatt atgctgatgt
      241 atcaggacag aaagcatttg tctttattaa aattgcccaa acaattctat gagtttacaa
      301 caggtttgtt tgcagaatta catggcgaaa tggttggaga atttttaaaa ttcttcaaaa
      361 aagacttcca tccttctgat atagatgatt attatttagc agaagaattc tttgcagata
      421 aagcttatgc ttaattctat aaacagattt aatttttaat ttttctttac agcccaattt
      481 ttgaatttaa aaattgggct gtattaattt aataaaatac aattcaaaag caaactttag
      541 cacataattt acacactcaa ttaattggta aaaagctata aatacactat atttgcacaa
      601 aaaaaataat tcaaatatta tgagcgactt tcttcaaaat ccgatggtaa aacaattagc
      661 aaaagcacta aatttacctt taccaattcc agcattatta aatagaaaca acaatgcgta
      721 tgctgatact gaatggcaat caaaaaaatc tgcactagta attacttcaa atgaagacca
      781 aattacacca aaaatattga gtgatttaaa acattttatt aatgaaggaa cactttatca
      841 agataaaatt gacactgtcg ttttatctgc tttaggcatc gaaaacattg atatgagtgt
      901 agagtttttt actaaggctc aattagcagt acagaaatta aacaaaaacg gaagagttgt
      961 tgtattatca aaaacaggcg atgatataga acaacaaact attcaaaaag cattagaagg
     1021 tttttcaagg gctttatcaa aagagttagg aaaaaatgga acaactgtaa atcacttaac
     1081 cattacagat gctccaacaa gaaatgttgc acgtgccatc ttatttttct tatcagacaa
     1141 atcctcattt atcacagggc aagtcgtaca attaaataat aacttttctt ctgaattaaa
     1201 ctcagtaagc aaatccctaa atggaaaagt agctatcgta actggtgctg ctagaggaat
     1261 tggtgcttct gctgctacat ttattgcaag agaaggtgca aaagtcatta ttgttgatgt
     1321 gcctcaagct aaagaagatg cggaaacatt ggcaaaagaa attggaggcg atgtattatt
     1381 aatggacatt accaaagaag atgctgtaaa aacaattcaa gattatgtat taaaaaatta
     1441 tacccaatta gatattttag taaataatgc aggaattacc agagataaga ctttaacaaa
     1501 aatgaatatt gcacaatgga catctattat gcaagtcaat ttaaaggctg caatgaattt
     1561 aacaaatgca tttattcaaa atggattttc taagaaccct aaaattgttg gactagcatc
     1621 aatatctggt attgcaggaa atgtaggaca aactaactat tcagcgtcaa aagctggaat
     1681 gattgcttat atgaacgaat tatctaaaaa aggttttatg gcaaatgcag ttgcacctgg
     1741 ttttatcgaa acaaaaatga cggagaattt accaattttt gttaaagaag gcggacgaag
     1801 attatcaact ttaaaacaag gtggacaacc tcaagatgtt gctgaattaa ttacattttt
     1861 atcaacacca ttatcagacg gaatcagtgg acaagtggtt cgtgtatgcg gtggtagcat
     1921 gattggagca taaaaaaatc aattacaagt acaaaatggc gtttatatga ctataaacgc
     1981 caattttatt taaaataatt aaaatttttt tgcttttaag ttttgttagt atgatttttt
     2041 tatttacttt tggtaactta aaaagtgtta aaaaaacaat ttgcaatatg aatacaaaat
     2101 caatcttaac tttagtagtt gcgtttttat tttgcatgcc tcaaattctt tcggcagcag
     2161 acgacattga taaaaacaac gaaaaaattg aaaaattaga agccgaaatc aaaagcttaa
     2221 aagctgagaa ccaaaatttg ttgaatgcct caaaatctga tggttcagga gcaaaactaa
     2281 aaataagaaa taaagtatta agaccagcag gaaaaacatg gtatatgtct gcttcagctg
     2341 gatacggtat tccatttttg ttagcaaata aaaaatctcc tttaaaggaa attggagaag
     2401 gtgactggca acaatcacct ggagcactat caagaaagcc attatttggt acaaatggtg
     2461 gtggctttgc attaaattta ggctggggcc acatgttcaa taaatatatt ggaattgatg
     2521 tcttacacac tttagcttgg catccagaat cattagatgc aagaatcaac actaaatcaa
     2581 catcattctt aggaacatct tcttattatg ctacacaaaa aacagcgaca atggcaatct
     2641 atgtttctcc acatttagta atgcattggg ataataataa gcgttttggt attacgggaa
     2701 aaataggtat ggttgctcca atttttggaa atgcgatttc tagagcacat attgatgata
     2761 actcaggaag attattacaa actttaaatg gtattccacc agttttaggt attcctttta
     2821 tccacatgaa attagatgca gttgcaaaaa ctaaattcca cccaacactt ggaattagtt
     2881 cttctattgc ttttgatgtt aaattagcta aaaatatatg gatgtttgca gaagcaagag
     2941 ttcaagcata taccattaat ttaatttcta caaaaatgtt tgaatttaaa atggaaacta
     3001 gtttaaaatt aggtgattta gaaatctcta ctaaagttgc agatgacttg attaaacaaa
     3061 tttctggtaa tcttttatca ttatcttctt taacaggtgg tttgcctttg aatattgcta
     3121 atgcatctga agccccaaga ttcttaacag aacgcagata tgttgatgaa atcactgaaa
     3181 actctaatac ggcaagatat ggtacaaaat ctatgattgg tggaccatta aactttactg
     3241 gcttagaagc tgttgatttg aataaaccaa tggacgagcc aggacaaaga ttcaatacat
     3301 caactttata ttttaatgtt ggtttaagag ttgattttga cctttgggat aaaaaaagat
     3361 cagaaaaaag aaaagaaaaa gcagctttat aaaaacacat actctaaata aaaaatgctc
     3421 caaaaaattg gagcattttt tattttatac aattgctaaa aatccttaaa aacatttgat
     3481 aaataacaaa ataaacttta gaatatttat tttagcaagt tgtaataaga atgactaaaa
     3541 gcacttctat aatcaaatta ttttgttgta tatgcatatt taccatttgc ataaatagca
     3601 gttttgcaca aaaattttat gttacttcct cattgaaaga agttcctgag aactttactt
     3661 ttgatatttc atttactgtt gaaaatggca atttagataa atttacacca ccaaatttta
     3721 atggttttga tgtatatggt ccttctcaat cacaaaatat tagtattatt aatggaaatg
     3781 tatctaaatc tttttcttat acttatacat tacaacccaa aaaacaagga agttttacca
     3841 tcaatccagc caatgctgtc ataaatggga agaccttctc aacacaacca attgcaataa
     3901 aagttatcgc tccacaacaa aagcaaacac aaaacagaag aagacagcaa aactatgatc
     3961 cttttgaaga cttttttggt ggggctgaaa gtcaaagacc ttcagaagct gaaatcaaga
     4021 aagaattaaa tgaaggcata tttgtaaaag taacgcctaa taaaactact ttatatgaag
     4081 gcgaccaagt tacactctcc tataaattgt atttccgttt acgttatgat ggattaaacg
     4141 ctaccaaaat gcctacttat aacggctttc tttctgaaga ttttgacatt cctatagacc
     4201 aacaaaaaga acccgaaatt gaagtctata atggaaaaaa atattatgtt caggaattca
     4261 aaagagttgc cttatttcct caaaagtcag gaaaaactac tatagaccca atgtcttttt
     4321 cttgtgtggt tggtgttgaa atacctaata atttcaaccc gtttttctca gacattgaac
     4381 cttatgaata taatttcaat tctaatgctg tgaacctcga agtgctgcca cttccgagtc
     4441 cacaacctaa gaattttagt ggagcagttg gaaagtttaa tttccaagct agttatgaca
     4501 aaacacaagt taaagttgga gagccaataa aactaaagat tgcatataat ggaacaggaa
     4561 atttaaaatt aattgctgct ccaaaattga ctttcccaga agaatttgaa gcttatgaac
     4621 ccaaattaaa tgaaaattat gcaacaaaag gtagtattgt atcaggtaca aaatcttttg
     4681 aatatattct tatccctcaa gatggaggca cttttaaact ccctgaatat gaatttggct
     4741 attttgattt ggataagaaa gcttatacaa cctttaaact tcctgaaact gaaattacag
     4801 tatctggaaa agccaaatta tcagaaaata tagtaaattt tatgaagcgt gagaaaaagg
     4861 ataaagcttc gacttcaata aaaggtaata tagaacaact ttcagacaat tcaattctat
     4921 ataattcaac catattttgg gctttaagtg ctgcaccgtt tgctatattt ttgattggat
     4981 ttctacttag aaaaaaagaa tatgatgctc cggaattaat attgttaaga aagaaaaaag
     5041 cccatacaat ggctttaaat cgaatgaaaa cggctaagaa attaatgacg aataacaatg
     5101 aaaaaggatt ttatgatgag gtgattcgag cattattaca atatgtttct gataaactgc
     5161 atattccaat ggcagattta tctaaagaaa acatcagctt gaaattatca aacagaaata
     5221 tttctgtgga aaaaacagaa caattaaaag aaatgcttac ggcatgcgaa atgtccttat
     5281 ttgcttctat taataagtcg gatagtatga aaacaacttt ccaaaatgct attgattgga
     5341 ttattaatat tgatgaagat ttaagtaaaa ttagttaaga tgaataggct gaaaatatta
     5401 atatttgtat tgtttgttgg aatattttat tttggattct ctaataatat caatcaacaa
     5461 attaatttgt cgaataagta tttcaaagag aaacagtatg aaaaagctgc gaatattcta
     5521 acaggattat tagaccaaaa aatatacaat agtacagttt attataatct tggaaattgc
     5581 ttctatcaat taaaagaata cccaaaggcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnna gttttgtaat tgctttaatg ttatttttta tttgctataa aaattacatc
     5761 tatcaaacaa aacaaacaca tgcaattgta atgcaaagtg gggataccta cccaagtcca
     5821 aataaacata aaaaatataa aaatggatta gaagaaggtt ctaaaattaa gttattggat
     5881 aatgatggtg gattttataa agttcagact agtaatgata acgttgtttg gattctaaaa
     5941 agccaagttg cagaatttta gattaccatt gaacaacagc acaatttttg aagtcggcgg
     6001 ctaattttaa ttgtaaggca tcagcatacg ctttattttt ataattgccc ataaacaatt
     6061 tataagtcgg ttggttggtt tcacttgcaa tttgatcaaa ataaataggt tgttgatatt
     6121 gtgccaacaa taattttata agttctttaa gctctactaa atcaaagaaa ttacctaaat
     6181 ctattccaaa atatcccgca gtaacttttt caagtataga taatttggtt aagtctaaat
     6241 ctttacgatt tagcatacct tcattattta cgttcaattc attattgata ggagcttgca
     6301 ttggagcagg agctggtttt tgttcgttat cttcaacaat aggaatattc atccaagaag
     6361 tatcttcttg cttaatctct ttcttatatt ctgctaatgc ttcagacgaa attgataaat
     6421 ctgctggaac ttcatctggc tcaaccacct caacagaaac ttttgttgta cccttatttt
     6481 taaatcctaa taaatctgca gctttagtag aaacatcaat cactctccct tttacaaaag
     6541 gtcctctatc attaatcttt acaaatacag attttgaatt ggataagttg gttactttta
     6601 ccacggtgcc taaaggtaat gttttgtgag ctgctgtcaa tttatattga ttatacaact
     6661 ctccacttgc agttttacga ccgtgaaaat atgaaccata ataagatgca ataccttcaa
     6721 aattatcagg ttttgcaaat gttgctaaaa ttgaaaagaa cgcgataata agtactataa
     6781 cttttctcat attgttttaa ttttttttat agactcagag attatacttt ctctaaaaat
     6841 ctgatgcaaa attacaacgg caagtttaag atagttttaa taggttaatg aattgcatta
     6901 aaggttcagt tagaatatca tgccctccct tgtaaaaaat ttgtgaaaac tttaattttg
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atgtaaatta aactttacat
     7081 aaacatcatc taaatattta atatagtcgt caatttcaga ttcacgctcc tcggttgtca
     7141 tccaatttgc accaacctta ccagataatc cattaatata gaatctattc aaggcttctg
     7201 gaactattac aaaatgttcc tgttcatcta aatctttaaa acgtttaatg aaattttcag
     7261 ctaaaaaccc atatccatgt gcgactaacc aaactacttt tgttgcctca gtatattttc
     7321 cgtagcttac tatttttgca gtcttcgtaa ttgtaaggta ttccttttgt atttccattc
     7381 actatcaaat atacttaaaa aacagtgttt catatttttt tttattaaca ttaacttttc
     7441 acaacatcta tctgtgtatt acactttatg aattcacttt taatttttgt atttttacaa
     7501 agttttaaca tcatgaggct atcttgaact tataaatgaa gtaatgagca atagtgttga
     7561 caatattaaa cttccaatag aagacgaact gaaaatattt gaaaaaaaat tcagagatgt
     7621 catgcgtagc catgtcgcct tattggataa agtcaattat tatatctatc aaagaaaagg
     7681 caaacaagta cgtccaatgt ttgttttttt ggctgccaaa attgtgggcg aagtaaatga
     7741 gaatacttat gtaggagcat cacttataga attgttacat acggcaactt tagtgcatga
     7801 tgatgttgtt gatgatgctt atgaacgtag aggtttcttt tctgtaaatg ctttatggaa
     7861 aaataaaatt gctgttttag ttggagatta ttttctgtca aagggattat tgtatgcctt
     7921 agaaactaaa aattataatt tgttacatat cacttcagat gctgtaaaac gtatgagtga
     7981 aggagaattg ttgcaaatgg agaaagcccg aaaattagac attgaagaag aaatctattt
     8041 tgagataatt aaaaacaaaa cagcttcatt gatagccgca gcttgtaagg ttggtgccgc
     8101 ttcagtaact caagatgagg cactcatcaa taaaatgcat aaaatcggtg aattaatagg
     8161 cattgcattc caaattaaag atgatttatt tgattatggt gaagcagata ttggtaaacc
     8221 acgtggaatt gatattaagg aaagaaaaat gaccttacca ttgatttata ctttgcaaca
     8281 ttctgataaa gcaaccaaaa aatggataat taatactgta aaaaatcata atgaagacaa
     8341 acaaaaagtc aatgaattaa ttaaatacat tattaataat ggaggtatag aatatactga
     8401 acaaaaaatg ttagaataca aaaatctagc ttatcaagcc ataaatgaac ttccagatag
     8461 tgattcaaaa caagcattta ttcaattaat agagtatatt acaaatagga attattaata
     8521 aaaatgaata actcaacaaa caaattaatt atacaaactt atacattaat tataggtatt
     8581 cttcttttat taactaaatt gatggcctat attttaagta gttctaatgc tattttaact
     8641 gatgccttag aaagtgtcat taatatactg gctggagcat ttggattata cagtttgttt
     8701 attgcttcaa aacctactga tgatgaccat ccttatggac acggcaaaat agaattaatt
     8761 tctgcaaata ttgaaggcac tttgattttt actgctgggt tagttatggt tttaaaatct
     8821 atttataacc taatgtttcc ggtagaactt aaatatttag attttggaat tgttttaata
     8881 gctggtacag gaatcgtaaa ttatattatg ggttggtatt ccaaaaaggc tggcaccaaa
     8941 tactcatctg ctacacttat ttctagtggt gttcatctaa aaacagatgc ctattcatct
     9001 tttgctatca ttgcaggttt aataattatt tatataactg gaatgagttg gttagacaat
     9061 atcctttcta tcatggcagg tttatttatt atttattcag gatttaaaat catacaatca
     9121 tctgctgatg gtattatgga tagagctgat tttattttaa tagataaaac cgtaaatgca
     9181 ctcaataaaa accgtaaaaa agaatggata gatattcata atttaagaat tgtaaaatat
     9241 ggtgaaaata tccatattga ttgtcataca acagtacctt ggtactggaa tactcaggta
     9301 atgcacgacg aactcaaaca tgttgaagat gccattaaag aagctattcc tacacgttta
     9361 gaaactttta tccatgcaga tccatgtatg cccaaaagtt gttctatctg tatgatagaa
     9421 gattgtgggg ttagacaaaa accatttgaa gaaaaagtag aatggacaat tacgaatatt
     9481 accaaaaacg agaaacataa ttctacaaat atcatttcaa ataataacaa cacacaataa
     9541 aatgaatatt ttacttttag gctctggagg tcgagagcat gcatttgcac aaaaaataaa
     9601 ccaaagttta ctatgttctc aattatatgt tagcccagga aatgcaggta ctgcaaaaat
     9661 ggcttttaat gtttcacttc ctgactttga atctataaag aaattttgca tagaacaaca
     9721 aataaaattg gtcgtagttg gtcctgaaca gctattagtt gatggtattt atgattattt
     9781 tgttgaagat gcaatcttaa aaaatatacc aattattgga ccctccaaaa aaggtgccat
     9841 gttggaaggc agtaaagcat ttagtaaaca gtttttaatc aatcataata ttcctactgc
     9901 acaatataaa gaaattacgg cagcaaatat agaagaagga tttacatttt tagaaacatt
     9961 acaagctcct tatgtgctga aagcaaacgg attggctgct ggaaaaggcg tgttgatttg
    10021 tcaagattta gctgaggcaa aaagtgaatt agctacaatg atagatggca aatttggtgc
    10081 tgcaagttca actgtgatta tagaagaatt tatgcatggc atagagtttt ctgtatttgt
    10141 actgacagac ggaaaagact ataaaatact tcctgttgct aaagattata aacgaattgg
    10201 cgaaggcgat accggattaa atacgggagg aatgggtgca atttcgccac catcttttgt
    10261 tgatgatgct attatgtctg tcgttgaaga aactataatc aaacccacta ttttaggctt
    10321 ccaaaaagat gagattactt acaagggttt tgtctatata ggtttaatgt tgacgccagg
    10381 aaacattcct aaagtagttg agtataattg cagaatgggc gaccctgaaa ctcaagctgt
    10441 tttccctaga attaatagtg atattgtaga agtatttatg gctttagcga atggaacact
    10501 taaagatgtt gaattttcta tttctaataa agtttgtgcc acaattattt tagcctcaaa
    10561 aggttatcca gaagaatttg aaaaaggtaa agtaattacc catattgaag atgtaaaaga
    10621 tgcttatgtt tttcatgcag gcacaacttt agctgcctac ttacctagac cttgttgtga
    10681 ggatgaatct gaggcaatca aaaatggaat tgtaaacaaa gacgcacata atattatttc
    10741 tactggtgga cgtgtattag ctgtttccgc attaggtaaa actatagatg aagcagttgc
    10801 tatcgccaat aaaaatgcag aaattatcca atttgaaaac aaatattaca gaaaggatat
    10861 tggttttgat atgtgatttt taattgagta gtaagacata agtattgagt agtaagaaaa
    10921 ctaatgccta ttgcctaatg actaattaac taaaaaaatc accaatcaga taattcaatt
    10981 ggattaccat taacagattt tcctttcagt acataatagc ctttatgatt ttcatcatta
    11041 tatattgtaa gcgttactcc tgaatctaaa ctagtccaat gtccattggg aacatcatct
    11101 tttctcaata ctacattatc ataaaatgta aaactatttt tgcttttatt gatacttcca
    11161 taaatttctg atttataaat ataatttttt ccatttatat ttattgtaaa agtataagtt
    11221 cctgttaggc tcagatcatc agaaaccgtg caacataaat gaatatcagc attacctgtt
    11281 tgtccattat aagttaatct ttcaaatcca ttataacatc tattaaagac ttgtttatat
    11341 tgttcattct ctttttgtaa tgtattttgc tgtgcattat cgttcaccca ctttccttca
    11401 aaaagaagtt ttccatattt gtcatacaat tttccataac catttctaac gctatttgaa
    11461 acagcaccaa catattttga gccgtcatga taattaactg ttccttgtcc tgatattatc
    11521 ccattactaa aatctccttc ataagtagtg ccatcctcaa aacgaaaaaa accttttcct
    11581 tgttgctttc cttcttgaat agttccttta taatatgatt tatcagaaaa ataaacataa
    11641 ccctctccat tccagacacc atcaccttta aatattcctt taaatgtctg tccattttta
    11701 taagtgaata aaccttcacc ttctttagta aattttaaaa cttcgcccac atatttatta
    11761 ccatcagcat aaacaatttc acctttccct gtaacttctc catttttgaa agtgccttta
    11821 taggttgatt tatttggtat gataatcaat ccttcacctt gccactgccc attcacgaaa
    11881 ttaccttcat aagtaaaaga agtattctct gtatcataat agatggcctt cccttttcca
    11941 tttttacaat ctccagacaa acaatctgct tgtacactaa acggaattaa agcaattgta
    12001 aatactaata agcagtttaa aatatttttc atcattgtag tttgatataa atatacaatt
    12061 tttcttaaat ttgaaaaaaa acagaatgtt aatgttgtat aaatattctg gatttatttt
    12121 tcctatataa cctaaaaatt tcatattgaa ttcaatggac acagatattt attttatgca
    12181 aaaggcttta gatttagcta aacaagcata taaaaataat gaagtaccag taggtgctat
    12241 tatttgtatt gaaaatacga taattggcaa gggttataac caagtggaat tattgcaaga
    12301 cccaactgca cacgctgaaa tccttgcaat tactgctgct tgtagttatt tgggcagtaa
    12361 atatttgaca caagctactt tgtacgtaac tttagaacct tgtcaaatgt gtaaaggtgc
    12421 tattgctcac gcacaaattc ctagagtggt ttatgctgca acagatactg caagaaagac
    12481 agatacttct aaaatttatg aaggaggttt acttgtagtt gaagcaactg aattaatgaa
    12541 acaatttttt agagaaaaaa gaatgtttta agtaaaagaa gtttctgaac ctactacatt
    12601 ttcataagca acataaaatg ctaataaact gactggcata gtaatcaaaa taccaatacc
    12661 aaaagccatt aaacctaaaa cattaagcat caataataca cttgcaaaca aaatccaacg
    12721 actaaatttt ttctttacaa ttaaaacact atataccaaa gatttgatta caggcatttg
    12781 ctgaaaccac aaaagataag aagccaagga taatagaatt gcggtgtata aaaaagtcat
    12841 catcattaca aagaacacaa ttctggtttt catagaaata actggcaact ctggaaattt
    12901 ggtttcattt aataagttag gattcgcaat tacaaaattc tttatatctt gaatatcttg
    12961 agaattagca ataggcataa aaaacaaaat caatataaat gtaaatatgg caatattagt
    13021 aaaatacaat agagcaatag gcactattcg ttggtagcca tcaaaaaaat atttaaaatt
    13081 tgattcttgt tttttatata ctttttcagc aaaaataaaa tagccaaatt gtaaaggtgc
    13141 taataaaaaa aataagaaac ctccaaattt gggaatacgc aatacttgct cattcgccat
    13201 catagccaat aaggtaaatg ttatataact aaaaaaagaa gctttgtata atccaaatcc
    13261 tgatttgata taatttataa actctaactg ataaccctct tttaaaattg attggattgt
    13321 tttattttct tccattaaaa attcattagt tcgattcttc acaaaattaa tctaaagcaa
    13381 tcgaaataaa acgaaagatt agttattttt tataaatagc aacttatttt ttaatgctat
    13441 tttgtttttc ttctgcttta aagatagaaa aaagcaaacc acccattaat gctccgtaca
    13501 atgtactatt tataggcttt gacgtgatag cacaagtacc tgaatcacaa ccaatattta
    13561 gccaatatag atatcctgct attgctccaa ctattacacc tatgatggta ataaaattag
    13621 atttaaaaaa actcatttaa ctattcattt taattttcca taaacctctc aattcgccta
    13681 attgataacc agttgcttta tctgttggat atttggattg aattacttgt aaagtttcag
    13741 gtgctatatc cgttgttgta tttccatggc aagataaaca agttggcata cctaacggaa
    13801 tgggtttata ataatacaca tttccagatt cttgcagtat taaatgttta tctgcaatag
    13861 ttgcatctgc catcattttc tttatttgtt ccaaagctga tttatctaat tccgaaacaa
    13921 ttgcattatt tgggtttcta tttttctcac taattcgttg tatgctaaca gcattcaaat
    13981 tcgatactga atctgtcaaa ggcatagcgt taatattaca aaaatcaaca gcacctgcta
    14041 cacctttttc ttgaattgca gcagaaacat ttttcattaa cacttgctgt gcttgggttg
    14101 cgatactatc tcctttggat aaataatctt gctgttcgga tagggtcagc gttttattat
    14161 tagtttgaca actaaaaact actagactta ttattccaaa aactaatatt tttctcatct
    14221 tttaaattta tcacttaata tcaatactta caaaataata tacaaaacag atagtatgta
    14281 caactcaaat gacctttata gactattcac aatttgacgt acatttgtcc aaggtccgcc
    14341 attaataaca ttagcaattc cgtttttatc taaaaatgct tttgcttgtc cacttcttcc
    14401 tccacttaaa caaaaaacta caatattagt ttttccttta aattttgaca attggctttg
    14461 cagtttgtcc aatggaatat taacggcacc ttttacactt ccaccagcaa attcgcctgg
    14521 cgtacgtaca tcaactagaa atgcaccttc ttttattact gtagctaatt gttcattacc
    14581 tgaacccatc atatttttaa taaatccgaa cataattata cttttaattt tgttatttaa
    14641 tagtacaaag ttctatcatt atgctttctt ctaccgcaac ttttgttaca caagctaatt
    14701 tttagttttt tttaagcatc tctacaattt taatacattc cttcgcctct tttacatcat
    14761 gtactcttag aatatttgct ccattttgca aggcaattgt atgtaaaact gtcgtaccat
    14821 ttaatgcttc ctgtggtgta ttttctaata atttataaat catacttttc cgagaaacac
    14881 caactaacaa aggatatcca agcaatttaa agaagtccaa ttgttttaga attgtataat
    14941 tatgttctaa tgttttgcca aaaccaaacc ctacatctac taagatatct ttgacaccat
    15001 aacttttggc tacatcaatt cgttcaacaa agtagtcaaa aacttctgta atcacgttgg
    15061 aataagttgg atttagttgc atggttttag gcgttccttg aatgtgcatc atcgcaattg
    15121 ggatattatt tcttgccgct atttcaaaaa tatttttatc tacaactcca gcataaatat
    15181 cattgataaa aactgcacct tcatttattg ccatctctgc aatggaactt ctgaaagtat
    15241 caaccgatac aatactttca gaaaatgttt tttttatcaa ctttaagata ggtaacaatc
    15301 gttctttttc aatttcaggc gtaacatctt cagcacttgg cttggtagaa acagctccaa
    15361 tatcaataat atcagctcca tcagaaagca ttttttcaac tttttttaaa atagcagttt
    15421 cagaattgta tctaccacca tcataaaaag aatctggagt aatatttaga atacccatta
    15481 cttttggttc tgcgaaattt aaaagtgtac ctccacaatt tagcacaaac gagttatttt
    15541 ctttaatatc aatcatatac aaaacttaca tcctataaaa aagctaaaaa ataatgaatt
    15601 taagtacaaa aattggattt aaaagccaaa gttatgtatt ttagttgcaa tttatgaaat
    15661 ccgaattagg aaaaacaata cagcaatatg atgcagcatt ttctaaatgc agaaatatat
    15721 ttattacaaa agtgagagat tatggaacat cgtggcggat tttacgcttg agttccgttg
    15781 ttgatcaatt atacatcaaa gccaaacgta tcagaactat tgatgagaaa ggcgaacaaa
    15841 aagttgacga ttctgttgaa gatgaattta ttggttgctt aaattatagt attattgctt
    15901 taatccaaat ggaattaaaa acagaaaata atgaaactat agaattagaa tttccaattg
    15961 tagaaaaact atatgataaa tactatcaac agattaaaga attgatgtta gcaaaaaatc
    16021 acgattatgg cgaagcatgg cgagatatgc gtatcacatc atttaccgat atgattctta
    16081 cacgtttgca tcgtattaaa caaatggaag ccaatcaagg tcagtctgaa atttcagaag
    16141 gaatagatgc caacttaaaa gacattgcaa attatgctat ctttgcacta attcaattat
    16201 cagaaaataa atagaaataa ataattatga aaattatcac acaggtttgt agatttttgg
    16261 tgggtgcctt atttattttt tcaggcgttg ttaaagctat tgatcccgca ggaactgcaa
    16321 taaaaatgga agaatacttt gaggtatttg caggctatct tccgaaatta aatttcttat
    16381 ggcatttttt agcccatcaa gcactttccg tttctgtttt tatgattgta ttggaaatat
    16441 tattgggtgt tactttaatt ctcggaatat ggaaaaaatt cactttatgg agtttagcac
    16501 tcatcattat tttctttaca ttcctaactt ggttttcagc aaaaactggc aaagtaacag
    16561 actgtggttg ctttggtgat tttatcaaat tagagcctaa acaatcttac cataaagact
    16621 tagttctcag tgtattaatt ctaattatca ttattggagc aaggaatata caattattat
    16681 tctccagaaa actttctact ataatttttg gaattttaac tattgctact ttattattta
    16741 catttagaaa catctattac gaaccaattg tagatttcag accttatacc gttggcactt
    16801 ccattcctga ttgtttgcaa ttacctcctg atgctaagaa gccaattact gaaatggttt
    16861 ttatttataa aaataaagaa acaggcaagg aagaacgttt caacaatact tttccgcaag
    16921 atattgacaa ttggactttt gtggatagag aagataaaat tattcaaaaa ggcgacgaac
    16981 ccaaatgtaa agattttgcg attagagatg ccaatggcaa tgataattca gcaggatttt
    17041 ttgaagaaga agaatacgtt tttctaatca tttgtccaaa tatcaaaaaa gcaagtgata
    17101 gaggttttga taaattattg cccgtttgta aagcagcaga aaaagaccac cgttatgttt
    17161 tcatgcttac tggaagtgtt ttagaagatg ttgaaaaata caaaatagaa cataaaattc
    17221 cttatgaaac ttacagtaca gatgaaacac ctttaaaaac aattatgcgt tcaaatcctg
    17281 gacttttgat tttgaaaaaa ggtgtgattg ttgctaagtt tcattataat gaattacctg
    17341 attatcaaac tatcaaaaaa gaatacttaa aataatatgg gtaaatttat cctcaataaa
    17401 ttagcttatg gcatattggt acttattgga gtagtattta ttgtattcgc tttgtttaat
    17461 ttattgcctg tcgatcctgc tcgtttaaca cttggacaaa gagctgatgt tgcatcggta
    17521 gaagctatca ataaagagtt aggattagac aagccatttt tagtaaaact aaaactatac
    17581 ttaaatgaca tttcacctat ttcagtttat gaaaatacac ctgaaaatca aacaaaatat
    17641 cattatacta aaattataag cttaggagaa agtgccttgg ttatcaaaaa accatattta
    17701 cgtcgctctt atcaaactaa agaattagtg agtgaaaaac tgagtaaagc attacctaaa
    17761 actataattc ttgccacatt ttctataata tttgcaagta ttatcggtat tttctttggg
    17821 gtattggcag caattcgcca gcactcttgg tttgatacca tagcgatgtc aatttccaat
    17881 ttaggaattt ctgtcccttc ttatttttca gcaattattt tagcctatat ttttgctatt
    17941 ttattacatg acattacagg cttagaacaa gtcggtgata ttacgaccat tgacgattgg
    18001 ggcgatgaag tcttgacttt aaaaaattta atattgccag ttttagcatt aggttctcga
    18061 ccaatcggaa ttatttttca attgacaaga agtgccatgt tagatgttct gtctcaagat
    18121 tatataagaa ctgctaaagc aaaaggctta tcgactaaaa tcgtattatt caaacatgct
    18181 ttacgcaatg ctatgaatcc tgttgtaacg actgtgagtg gttggtttgc aggattatta
    18241 gcaggtgcct tttttgtcga aataatcttt gacattaaag gattaggata tatagcagtc
    18301 aattctttat taaactttga ttttcctgtt acgatgggtt gtgttttatt tactgccatt
    18361 gtgtttattg ttgttaattt tattgtagat attctatatg ggattttaga cccacgtatt
    18421 agaattggaa ataaataatc tttatacttt atgttgattt tcttagttgg ttttatgggt
    18481 tgtggaaaat cctatacagg tcggaaaatt gcacctattt taggctatca ttatgtggat
    18541 atggacaaag tgattgaagc acaagaatct atgtctatca aagaaatttt tgaacaaaaa
    18601 ggtgaagcct atttcagaaa tttagagcat caatatttat tagatttaga tacagatcaa
    18661 aacatggtta tttctacagg tggtggtgtt ccatgctttt ttaataatat ggaaattatg
    18721 aacactaaag gaattactat ttatctgaat aggagcaaat ctgttgttat tccgaggcta
    18781 ttacaaggtg cacacaagcg accactcatt caaaatatgg atgaagaaac tttgaatact
    18841 ttttatgtta caaaattagc agaacgtgct ccattttatg aacaagcaca attacacgtc
    18901 ggcgatgcta atgtggaaga aattctgaag ttactagcga taaactaaaa aaccaaacaa
    18961 tcagaattta cgaacattga tttataattt tagtatctct gttttataat tcatagcttc
    19021 aattaggaac tcctattaag tttgagacta attcagttca aatcccagta tatattttac
    19081 atattgttag aaaatacgaa atacaatatt gcgtattata ttcataaata caggtaatac
    19141 gcaataagac atctgtatat atttaattag cttacaattt gttagcattt taatgatact
    19201 tttttttgat aatttaaaat catttattat atttgcgtat ataaaggagt tatcggtaac
    19261 tctatgacga cagtgctaac ataaaacaaa ggactaaaaa tgaggaacag agcgattttc
    19321 ggcaaagccg tttggctatc cttcgcaaaa gcaaaagaga agcaacgcca cccacaagcc
    19381 aaagcaagtt gcttcacatt tgcttttgcc caaacgcaca atgacattac gacaataata
    19441 ttcaacataa atcaagatat ttgaaacaaa taaaatgaca atggaacaag caataataat
    19501 tatagcggca atgacacttc ttggtgtcat tgcacttatt tttctttacc taacaactga
    19561 aaagaaaaag aaagtcgcaa gagaagccga gctaaccgaa ctaaaggaac ttttgaaaac
    19621 agaagtcaac aaccttctga ttaaagttca agcaaaaatg caagagacca caaatcaaga
    19681 gacagaaatg tttgaagcac ttgctaaaga agttcgaact gcacaagacc aagcaaacgc
    19741 aaaaattaat tctgtttttg aagaattgca gtccgagaat acaactctaa gaaaagatgt
    19801 tggcgaaaaa attgctgaca tcaaatcttc tttcaaagaa tactcagaaa aggtaaaaga
    19861 taacttgaat aagtactcca acgataatgc agagtttaaa aaaggaacag agctcttaaa
    19921 agagcagata caaagagaac ttcaaaacat tctaaaagaa atcaaatctc ctcttgacat
    19981 agattaagac ctatattttg cataatcatt cacaaaatac gcaacgaata attgcttatc
    20041 tctcttacct tagagacata agcaggaaaa tcatttcacg aaatggtttt gaaaaatgct
    20101 tgcttaacaa atcattgggc tatgcttatc agtttgaaaa tttagaactt gagttaaagg
    20161 ttggagaaag ccaatacgaa gaacttaacc gatattttag ctttcttcaa aagaaccacg
    20221 ataagaaatt
//
LOCUS       DAOQCW010000035        20214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_962_length_20214_cov_6.049308, whole genome shotgun sequence.
ACCESSION   DAOQCW010000035 DAOQCW010000000
VERSION     DAOQCW010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 20214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20214
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_962_length_20214_cov_6.049308"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PLS16_04685"
     rRNA            <1..204
                     /locus_tag="PLS16_04685"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            250..359
                     /gene="rrf"
                     /locus_tag="PLS16_04690"
     rRNA            250..359
                     /gene="rrf"
                     /locus_tag="PLS16_04690"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(509..1129)
                     /locus_tag="PLS16_04695"
     CDS             complement(509..1129)
                     /locus_tag="PLS16_04695"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNJ60408.1"
                     /translation="MTNDVLIPKDGWINIQSPIEIPNLDKQLWFKLSVSVAKTFGRTE
                     IPNVFKLLAINQDIFWRWLFFASRLMPYGELTDREREIIILRVAWLCRCRYEWGQHIE
                     IGQNKAKLSDEDIVNISKGINAFANPKEQLIIKACNEFINKKAISTETWNLLSKYYNN
                     KLMIEISVLIGHYEMLAGILNSTGLQLEPKIEQYYKMFNERIKGME"
     gene            complement(1237..3405)
                     /locus_tag="PLS16_04700"
     CDS             complement(1237..3405)
                     /locus_tag="PLS16_04700"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNJ60409.1"
                     /translation="MAVIGKIRERSWILLTFLAVSLFIFIIQSALESPSGMFKGGDKD
                     SVGEIDDNNISATEFSKDVTDYEEGLKLINPNVTLDDNLRTQINDEVWLNMAKENLLD
                     PHLKELGIEITTKELGEIMWGSNAHPLARNFWAQVGAVNQETGEIDEAKAKDFIKNID
                     KNDPNGENKLRAVIEQIEKLVMEETIKMKYTYLISKSYYMPSFFAKEVVNASREGVAT
                     VLSVPLNSIPDTDFKVIDADITKYISENPNKYKQEASRTLDIVTFDIIPTQQDSLDRK
                     TKLEELRNAYLAITDKSKDTSFIERNSIQREQIAYYSKQDLTKSGRNADEIFSLPIGT
                     TTPIYIENGSYVFTKILDRRIAPDSVHAAHILLSLGNKTEAEIASANKKADSLIQVLA
                     AGKANFGDLANQNSIDENSKIKGGDLGYFTRNQMVKEFNDQVFFKNMSPGNLAKVETQ
                     FGLHIILLLDARNPQLLTEFADFVETIRPSKETDSKAYAQATEFLQKNNTSAKFDAAA
                     KTEKVSKNIIVTSNSSNIQNLGTSKDLIRWAFDEKEGKVGNIKFFDMSDKFIVAKINK
                     VSEKGLAKASDVREEVTQILINKKKADKIAYDVEKAADKKTDLASIATELKDATIKDS
                     VKVTFTSGFSEIGNEPKLSGAVFGTPIGKISKVIKGNEGVYLVQPSTIQQSSTLLESN
                     SLETYQKQMQQMASQQINYQEIINSILDKVEVKDKRYTFF"
     gene            3718..4119
                     /locus_tag="PLS16_04705"
     CDS             3718..4119
                     /locus_tag="PLS16_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HNJ60410.1"
                     /translation="MKKIFLTLLVTISLVAAGFAGNTLKLNVKGMGGSCCENSFKAKA
                     EKLVGVIQVSGVSASSKSAVIEYDAAKTNENEIISTLTKQTGYTIAATTSDAGSNVAP
                     KSCCSKPGATKCATTETGKNCTKSKSSCTTK"
     gene            4246..7146
                     /locus_tag="PLS16_04710"
     CDS             4246..7146
                     /locus_tag="PLS16_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNJ60411.1"
                     /translation="MTYVSATFIQAQAYDSENKKSLPPFLYYEKSEWVDSVLNSMTLD
                     EKIGQLLMVAAYSNKDETHYQEIEQYIKKYKIGGLIFFQGTPEKQAELTNRYQAISKT
                     KLLIGFDGEWGLGMRLKNVVNYPRQLMLGAIEDENLIYDMGAEFARQMKRIGIHVNFA
                     PVIDVNNNPSNPVINDRSFGDNKYNVTLKAKAYMKGMQDNNLLACGKHFPGHGDTDVD
                     SHYGLPVLQHSIERLDKIELYPFKALIRNGIGSIMVAHMNVLALDNTPNLPSTLSKKI
                     VQEKLIDEMGFKGLIFTDALNMKGVSANYPSGVAEVKAIQAGNDVMLFPENVDKAIAG
                     IKAAIDSCELTEERINESVRKVLLTKYWVGLNNFQPIKMSNIVEDLNNEKANLLLTKL
                     VEHSITVVEDKNDFIPIIDVREKIAHVAIGIGSPNVFSSRIDDYAAIDHFYISKSESQ
                     LKFNQLIEQLKSYDKIILEINDMSRFLAKNYGLTDVEIGAINKINQSKKTINIIMGSP
                     YSLQKLNGYGSIIVAYNEDSVTQDITAQILFGARAGSGTLPISVANYEFSKGVCTNGS
                     LRLGYGFPIEVGMNAEILKGIDKIANEAIENGATPSCQILVAKDGKVVYRKSFGTMTY
                     KDSKLVDNNDLYDIASITKVVASTPIIMRMYEDKKLDINKTLGTYYNYPEGSNKTNLT
                     IKNILLHEAGLVAWKPFYKSLLDSVNQIKPEWMSKTYSPMFTRKVIDSVFIRNDFIDS
                     IRNTIYFSELGPKVYKYSDLGYYLLKEIIEKTYADSLQNVAKRYLWSFLGMNNTMYNP
                     LAAQVNLNNIVPTEDEKGFRNTLIHGYVHDQGAALMGGIAGHAGVFSNTNDLAKYFQM
                     LVNGGTYGGTRFYNKKTIDYFTSKQSSISRRGIGFDRPEPSGGGPTCSSASSLSFGHT
                     GFTGTIAWADPKYDLVYILLSNRINPSAENRKFINQNYRTRIQQVIYNSIQQ"
     gene            complement(7175..8389)
                     /locus_tag="PLS16_04715"
     CDS             complement(7175..8389)
                     /locus_tag="PLS16_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60412.1"
                     /translation="MEKEIKTIFKNWFWWGFLSLVVVAFYGTLMRYKIAFSFPFFEQK
                     NLLYAHFNFAFSGWINHALYCGLAYLIFPFLSSANQKKYKWLIVFNLICAYIAALLFT
                     FIGSKTDTIIFSALSTLIIAILFAVFYIRDNKLIPKDNLFSSWAITGLLLNILSVVGP
                     FLLAYMMVSKTFTQDKYLIYKYYFLHFQYNGWFFFASMAMLTTILPKNFPNLNQYFKV
                     FAATVIPTFFLSILWFKLPLWLYIITVITTIIQMIAWLYFIKKYIPLLINDKKPSWVH
                     IFMYAATFAMTVKFILQTISIIPSLSHFVFGFRSIIIAYLHLILLGVYSLFIIGYSLY
                     NGFLSNSNSTKFATIAFLVGVVLNELVLGIQGAAGLIYLPIPHINEALFAVTLLLFGS
                     SIFIVISQKIRK"
     gene            complement(8391..8993)
                     /locus_tag="PLS16_04720"
     CDS             complement(8391..8993)
                     /locus_tag="PLS16_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNJ60413.1"
                     /translation="MHIDYNILIAYGGIAKRINKGDFIFKQDSLPLFYYQVIKGEVRI
                     FSSNTEDKFLTHNIVSEGEGFGESELLLNKPHLVSAQALSAGVFVRIPKENFNNILKD
                     FPDINQQLLHSLAEEIYDNILLSQIWTAPTPEIKIELFLNRYKKQNQEKDKPEEKILI
                     PYTRQQIADFTSLRVETVIRTLRKMSEQQKVKIVQHKVYF"
     gene            complement(9115..9615)
                     /gene="azu"
                     /locus_tag="PLS16_04725"
     CDS             complement(9115..9615)
                     /gene="azu"
                     /locus_tag="PLS16_04725"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HNJ60414.1"
                     /translation="MKKVFLTMTAAAFLFTACNNSSSTTENTTPTTEETAPAEDANVA
                     EVELSAGDDMKYDLTEIKVKEGQTVKLTLVHTGTAPVVAMGHNFVLLKQGVDLAKFAE
                     AAMAAQATDYIPADKAGDVIAHTKTIGGGETTEVEFPAPAKGTYEFLCSFPGHSAMMK
                     GHFIVE"
     gene            complement(9649..10371)
                     /gene="ric"
                     /locus_tag="PLS16_04730"
     CDS             complement(9649..10371)
                     /gene="ric"
                     /locus_tag="PLS16_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HNJ60415.1"
                     /translation="MNITKESTIAELVAQDYRVAAVFKDKAIDFCCNGNRSIQEVCNN
                     QNLDADQLINELTATMGQGGAANIDYNSWPLDLLADYVEKKHHRYVEKAIPEILPYLE
                     KITRVHGGRHAELEEVLALFNEGAGELTKHMKKEELILFPYIRKMVKAEAAKTTVEAA
                     FGTVQNPIEQMMHEHDTEGDRYRKISALTNNYTVPEDGCNTYRVTLSLLKEFEDDLHL
                     HIHLENNILFPKSILLEKQLNA"
     gene            complement(10399..10797)
                     /locus_tag="PLS16_04735"
     CDS             complement(10399..10797)
                     /locus_tag="PLS16_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HNJ60416.1"
                     /translation="MSTIKQIENIEDVKLLVDTFYGKVREDEILKDVFNNVIQNRWPQ
                     HLEKMYRFWQTVLLDDERTYFGAPFPPHAQLPVDAIHFERWLKLFYGTVDSIFIGERA
                     ERAKWQGNRMAEMFLYKIQYYRNNDVIPLA"
     gene            complement(10864..11094)
                     /locus_tag="PLS16_04740"
     CDS             complement(10864..11094)
                     /locus_tag="PLS16_04740"
                     /inference="COORDINATES: protein motif:HMM:NF022969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexameric tyrosine-coordinated heme protein"
                     /protein_id="HNJ60417.1"
                     /translation="MGIWLESLITATPQEGRELAIKMSRKTIAAIQTDPEERKKLRVN
                     YASDTAQLIASANVVAIEFQTIAQANNYWKKG"
     gene            complement(11123..11557)
                     /locus_tag="PLS16_04745"
     CDS             complement(11123..11557)
                     /locus_tag="PLS16_04745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNJ60418.1"
                     /translation="MFSKSCEYGIRALTYIATESLEGKRVKISDVAENADLPEAFTAK
                     ILGTLTKQNIVRSLKGPTGGFEIPLDKMKQIKISEIVFAIDGDSIYNGCALGLSECNP
                     DAPCPMHDQFIKVRGELKTMLTTTTIFDLATKIKTGKSILVR"
     gene            complement(11655..13019)
                     /locus_tag="PLS16_04750"
     CDS             complement(11655..13019)
                     /locus_tag="PLS16_04750"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNJ60419.1"
                     /translation="MNNRIKDIRFYVLSDYIAACITWIIFFSFRKIYIEELHFQKSFL
                     TNDTNFLKGLLFIPVFWWICYFTSNAYTDIYKKSRLNEVGKSIFQSIIGTFFLYFLVM
                     LNDRIRDYQDYYLLFTAYVSIHLLTLLTGRLLILNYAKNRIIHKKVQIETLLVGEAKD
                     RNQIKSILSTQSLPILSHYIKNEVDFENAHTLIHQNQYEEAIIIAHQYEHQQVEHLII
                     ECLAKGILVKIKANDFDILSGKYKTQSIFESGFIEISPFVLTPFQRVTKRLFDIVLSF
                     LVLILLSPFLCIIAIIIKKTSTGKILYTQERIGKNSQPFNIYKFRSMIENAEGDMPQL
                     TQENDLRITKIGSFIRKYRIDELPQLFNVLKGDMSIVGPRPERRYFIDKISKTTPKYQ
                     LLQRIKPGITSLGMVKFGYAHNVPEMIQRMRYDILYIENMSISLDLKILFYTVSTVLQ
                     GKGK"
     gene            complement(13031..14386)
                     /locus_tag="PLS16_04755"
     CDS             complement(13031..14386)
                     /locus_tag="PLS16_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNJ60420.1"
                     /translation="MPKILIVDDEKAIRRTLRDILEYEKYEIDEAEDGEIGVQKIEKG
                     NYDVVILDVKMPKKDGIEVLAELQNKGIDTPVIVLSGHGNIETAVEAVKKGAFDYIPK
                     PPDLNRLLITVRNAMDKSNLVSETKILKKKIAKTKDIVGTSNSIQKIKDTIEKVAPTD
                     ARVLITGDNGSGKELVARWLHEQSNRANGPLIEVNCAAIPSELIESELFGHEKGAFTS
                     AIKQRIGKFEQANGGTLFLDEIGDMSLSAQAKVLRALQENKITRVGGDKDFTVDVRIV
                     AATNKDLLKEAEENRFRLDLYHRLSVILIHVPSLNQRKDDIPMLSEHFLQEICGEYGV
                     PTKKIEKDAVEALQARNWTGNIRELRNVVERLIIMSGKSITKQDVLDYSGPNKKIEQA
                     GIDFDRFANLQQIQDYTERSFIEHHLDKNDWNISKTAEQIGATKKQLEEKMENLGLKK
                     R"
     gene            14467..15282
                     /locus_tag="PLS16_04760"
     CDS             14467..15282
                     /locus_tag="PLS16_04760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ60421.1"
                     /translation="MAKLDLGAVQETLLLPLAARAYDAESKYPILNDQKALELFKQLV
                     PNRKAFRKNLLKVGIVSLTERGWIMDRAVKDFLKRNPKGKVLNIGAGLETSFYRLGQP
                     NVPWFDLDLPDSLALRKQLLPNNFKNVKYIPKSMFDESWIDDIGNISDGLMITVPGVF
                     PYFDEKQVQLFFNTFAPKLKGAEIIFDVLSNATRFFVQRKINQAGMSKATLDWGIMNP
                     RHIEKWNQHIQFVKAYSYLQHPSFNAIGNPIYNFISWNSKFFKSAQIFHFKFV"
     gene            15292..15921
                     /locus_tag="PLS16_04765"
     CDS             15292..15921
                     /locus_tag="PLS16_04765"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HNJ60422.1"
                     /translation="MDVYFIHIDNFIWSETMENDIGSFPENIHQKINAYKSIEHKIHD
                     YYGKKIIQFLLQKYQPNYALCDLKYTEKGKPYFVGDFDINISHSGNFVVGVYVENAQV
                     GIDIEKHRNIEALDFKKYFSENEWNTIQHSENPLITFFDFWAIKESAIKCNGNGFKVL
                     SKTQTFDEKSIQCVDEIYNYRQITIDNNYSCSVCCNNKIETINLIEISL"
     gene            complement(15924..17537)
                     /locus_tag="PLS16_04770"
     CDS             complement(15924..17537)
                     /locus_tag="PLS16_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNJ60423.1"
                     /translation="MKKNTLVLVWIGLLCLVACKETHKEKSAKFFHLNISAGLTSLDP
                     AFSKDQATMWCANQLYNGLVQIDSNLNILPSIAKSWTISNDGLNYTFILRNDVFFHDH
                     ELFPNGKGRKVLASDFVYALTRIIDTTVASTGAWLFNGKIQEQNPFEAINDTTFVIHL
                     KEPFRPMLGLLTLQYCSVVPKEVVEHYGKDFRKHPVGTGPFYFVRWEENNVLIVHKNK
                     NYFEKDSNGNRLPYIDGVRFNFIANRNSEYNQFQKGELDFMTGLDISFKDKLLTKEGV
                     LKSEWKDKINFLKMPYMNTEYIGISMAKQPCAALKNKKVRQAMNYAIDRKKMITYLRN
                     GIGIAAESGIIPLGMNEFDSTVVKGYHFDLSKAKQLLIEAGYPNGKGIDQITVYSNPT
                     YQDLISSVANQLAEIGIKIKIENSPAAFLREAMRKNEAQLFRASWIGDYPDAENYLAL
                     FYSGYGAPPNYTFYKNPAYDKLYEKAMQTNNTVDALKMYHQLEQMALEDAPVILLFYD
                     QIVRFTHKKIKNLPSNAMNLLDVKTVQID"
     gene            complement(17596..18558)
                     /gene="pfkA"
                     /locus_tag="PLS16_04775"
     CDS             complement(17596..18558)
                     /gene="pfkA"
                     /locus_tag="PLS16_04775"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNJ60424.1"
                     /translation="MKNIAVLTSGGDAPGMNAAIRAVVRIALLEKLKVFGIYNGYQGL
                     VDNQIQPLNKKSVGNIIHRGGTILKSARSKDFQTKEGRNKAYHNLVEHQIDAVIVIGG
                     DGTFAGAIQFTQEFNIPFVGIPGTIDNDLFGTEYTIGFKSAVQTAMECVDKIRDTANA
                     FKRLFFIEVMGRDVGAIALATGIATGAEDIFIPETKTNLNDLSERLKRGTDKESYIII
                     VSEGDDKGGAENIAAEFKKTHPEYDIKTLVLGHLLRGGNPTAEDRILAARLGVAAVQS
                     LIQKEFNVMIGIQRNEIAKTPLHLCKKHFLKINQEWEDLIYKLS"
     gene            complement(18558..19412)
                     /locus_tag="PLS16_04780"
     CDS             complement(18558..19412)
                     /locus_tag="PLS16_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HNJ60425.1"
                     /translation="MKIAESELILNKDGSVYHLTLLPEDVADTIITVGDPERVPMVTK
                     YFDKIEIQKANREFVTHTGYLGNKRISVIATGIGTDNIDIVLNELDALFNIDLRTRTP
                     NEQLKSLNIIRIGTSGALQNDIPMDSFLVSEYAVGLEGLLHFYQYHNAHNAINDAVSK
                     ITNTESYTTQADTYLLDKLAKGIKRGITLTAPGFYAPQGRQLRLQPKQKDFIDTLSAL
                     KFENDLQITNLEMETSGIYGLGQLLGHRCISFNAMIANRQLGTFSVNAEQTIDSLIRF
                     VLENIKHL"
     gene            complement(19443..>20214)
                     /locus_tag="PLS16_04785"
     CDS             complement(19443..>20214)
                     /locus_tag="PLS16_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60426.1"
                     /translation="LKNRKKIVRLHNIEWLYYGKLLDLALGIKDKIYFYFEYKKLIKY
                     QSILENSQALHCISSSDTAYYTNRFPNIPTTFIPAFHQNTEIKTKIGKGEYILFHGNL
                     SITDNYLPIVRLLRNQLKNCQFPIIIAGKNPTEHLIETVQLFKNIQLISNPTHEDLET
                     LIQNAHINIIFSEIETGVKLKLINTLFLGRFCFTNQAAVIGSELQNATILVNENDIEQ
                     QIKDYFQQEFTTEILTERIKYLSLYNNKDNALQIINNL"
BASE COUNT         6566 a         3074 c         3180 g         7394 t
ORIGIN      
        1 cctctagtgt accggttatg tcgccagatg taccgccggg tagctacgtt gggaagggat
       61 aagcgctgaa agcatctaag cacgaaaccc acttcaagat tagttctcat agggccgtca
      121 aagactatga cgttgatagg ctacaggtgt aaagatggta acatcaaagc cgagtagtac
      181 taatagcccg ttcattttta aaaattattg attattttcc cattatgttc aaaaatatat
      241 ttaataaata atggcgatta cagcaagggt gttcacctct tcccatcccg aacagagaag
      301 ttaagcccct tagcgccaat ggtactgcct tatggtggga gagtaggtca tcgccaacct
      361 tttatataag cctcatagtt ttctatgggg cttattttat tttagcttta tacaatcttt
      421 atttgttatc tctctttatt attgtcttgt aaaaattgaa gatattggta gtttgatatt
      481 gtacaaatat gttcttgttt gggatttatt attccattcc tttgattctc tcattaaaca
      541 tcttgtaata ctgctcaatt tttggttcta attgaagacc tgtactattt aatatccctg
      601 cgagcatttc ataatgacca attagtacgc ttatttctat cattagtttg ttattataat
      661 attttgaaag taggttccag gtttctgtgc ttattgcttt tttattgata aactcattac
      721 atgctttaat tattaattgt tcttttggat ttgcaaaagc gtttattcct ttggatatat
      781 ttacaatgtc ttcatcgctt agtttggctt tattttgacc tatttcaata tgttgccccc
      841 attcgtaacg acatctacaa agccaagcta ctctgagaat aattatttct ctttctctgt
      901 ctgttaattc accataaggc attaatcttg atgcaaagaa tagccatctc caaaagatat
      961 cttggttgat tgctaacaac ttgaaaacat taggaatttc ggttctccca aaagttttag
     1021 caacggatac tgatagtttg aaccataatt gtttatccaa atttggaatt tcaattggtg
     1081 attgtatgtt tatccaaccg tctttgggta ttagtacatc atttgtcata aagtgaagtt
     1141 agcaatatat ttttaatcgt actactttta aagatatact taaataaaaa agccttcgat
     1201 taaatcgaag gcttaaatat tgttttagtg ttttatttaa aagaaagtat accttttgtc
     1261 ttttacttcc actttatcta aaattgaatt aataatctct tggtagttta tttgctgact
     1321 agccatttgt tgcatttgtt tttggtaagt ttctagagaa tttgattcta ataatgtaga
     1381 ggattgttgt atggttgaag gttgaactaa ataaacgcct tcgtttcctt ttattacttt
     1441 agaaattttt ccgattggag ttccaaatac agcaccagat aacttaggct cattgcctat
     1501 ttctgaaaac ccagaagtaa aggtaacttt tacggaatct ttaattgttg catcctttag
     1561 ttctgttgca atactagcta agtctgtttt tttatcagca gctttttcta catcataagc
     1621 aattttatca gctttctttt tattaattaa aatttgagta acttcttctc ttacatcgct
     1681 tgcttttgct aaaccctttt cacttacttt gttgattttt gctacaataa atttatcact
     1741 catatcaaag aatttgatat tgccaacttt tccttctttt tcatcaaatg cccaacgaat
     1801 taaatctttt gatgtgccta aattttgaat atttgatgag ttagatgtta cgataatgtt
     1861 ttttgaaacc ttttctgttt tagctgcagc atcaaatttt gcagaagtat tgtttttttg
     1921 taaaaattca gttgcttgag cataagcctt actatctgtt tctttacttg gtcttattgt
     1981 ttctacaaaa tcagcaaatt cagtcaataa ttgagggttt cttgcatcta ataataagat
     2041 gatatgtaag ccaaattgtg tttctacttt tgctaaatta ccaggtgaca tatttttaaa
     2101 aaatacttga tcattaaatt cttttaccat ttggtttcta gtaaaataac ctaaatctcc
     2161 accttttatt ttagaatttt catctattga attttgatta gctaaatctc caaagttggc
     2221 tttcccagca gctaatactt gaattaaact atctgctttc ttattagcac ttgctatttc
     2281 agcttctgtt ttgttaccta aagatagtaa gatatgagca gcatgaacgc tgtcaggagc
     2341 tattcttctg tctaatatct tagtaaaaac ataactacca ttttcaatat aaattggtgt
     2401 tgttgttcca ataggaaggc taaaaatttc atcagcattt ctgccacttt ttgtaaggtc
     2461 ttgtttggag tagtaagcta tttgttctct ttgaattgaa tttctttcaa taaatgaggt
     2521 gtctttgctc ttgtcagtaa ttgctaggta agcatttctt agttcttcta attttgtttt
     2581 tctatctaat gaatcttgtt gtgttggaat aatatcgaat gttacaatgt ctaatgtacg
     2641 agatgcttct tgtttatatt tgttaggatt ttcactaata tatttagtga tatctgcatc
     2701 aatgacttta aaatcagtgt ctggaatgct gtttaaagga acactcagta cagttgcaac
     2761 accttctctt gaagcattaa cgacttcttt tgcaaagaat gaaggcatat aataggattt
     2821 agaaatcaag taagtatatt tcatctttat tgtttcctcc attactaatt tttcaatttg
     2881 ctcgattacg gctcttaatt tattttcacc gttagggtcg tttttatcta tattttttat
     2941 gaaatcttta gctttagctt catctatttc tcctgtctct tgatttactg caccaacttg
     3001 tgcccaaaag tttctagcta gtggatgagc atttgaaccc cacataattt cgcctaattc
     3061 tttggttgta atttcaatcc ctaattcttt taagtgtggg tctaagagat tttctttagc
     3121 catatttaac cacacctcat cattgatttg ggttcttaaa ttatcatcta aggttacatt
     3181 tggattaatc aattttaagc cttcttcata atctgtaaca tctttagaaa actcagttgc
     3241 tgaaatgttg ttatcatcta tttctccaac agaatcctta tctccacctt taaacatacc
     3301 tgatggcgac tccaatgcag attggatgat aaaaataaat aatgaaactg caaggaaagt
     3361 taataatatc catgaacgtt ctctgatttt gcctataact gccattctat ttgaaatttt
     3421 tgagtgtcaa agatagtgat tttaaattaa aatctttaat ttatttggtt gaataaagtt
     3481 atatttgtat ataagatagt tttttaattt taagagctta ttatttgtaa attattgtaa
     3541 ctgaaaaaag tatgaatcct tgatttattg tagatttgca cattagattt gggtatcatt
     3601 tgcttacttt taggtataat tggattttaa aagaatacaa tccacttgtg gcattgtctt
     3661 tgctgttctt tttattagtt ttaatgatta gattaattca ctttaaataa atgtaatatg
     3721 aaaaaaatat ttttgacttt attagttact attagtttag ttgctgctgg ttttgctgga
     3781 aatacgctga aattaaatgt aaaaggaatg ggtggtagtt gttgtgaaaa tagttttaaa
     3841 gcgaaagctg aaaagctggt aggtgttatt caggtttctg gggtaagtgc atcttcaaaa
     3901 tctgctgtga ttgagtatga tgcagccaaa acaaatgaga acgaaattat atctacatta
     3961 acaaaacaaa caggttatac aattgctgct acaacttctg atgcaggaag taatgttgct
     4021 ccaaaatctt gttgttcaaa acctggagcg acaaaatgtg caacaactga aactggaaaa
     4081 aattgtacta agagtaaatc ctcttgtaca actaaataaa atttttattt aactttttta
     4141 agaattggaa cagcaaataa actgttcctt tttttatttt taaaaacaat ataaaactac
     4201 taatttgcta aatacatgat tcgattctta ttcacttttg ttttgttgac atacgtttca
     4261 gcaactttta ttcaggcaca agcttatgat tctgaaaata agaaaagttt gccaccattt
     4321 ttgtattatg aaaaaagtga atgggtagat tctgtattaa attcaatgac tttagatgaa
     4381 aaaattggtc aattattaat ggttgctgcc tattccaata aagatgaaac acattatcaa
     4441 gaaatagaac aatatatcaa aaagtataaa attggtggat taattttctt tcaaggtaca
     4501 ccagaaaaac aagctgaact tacgaatcga tatcaagcaa tcagtaaaac aaaattgcta
     4561 attggttttg atggagaatg gggattgggt atgcgtttaa aaaatgtagt caattatcct
     4621 agacaattaa tgttgggagc gattgaagat gaaaatctaa tttatgatat gggtgctgag
     4681 tttgcaagac aaatgaaacg aataggaatt catgtaaatt ttgcacctgt gattgatgta
     4741 aataacaatc cgagtaatcc agtcattaat gatcgctctt ttggagataa taaatacaat
     4801 gttactttaa aagctaaagc ctatatgaaa ggtatgcaag acaataatct tttggcatgt
     4861 gggaagcatt ttccaggaca tggagataca gatgttgatt ctcattatgg tttgccagtt
     4921 ttgcaacatt caatagaaag attagataaa atagaattat atcctttcaa agcattaatc
     4981 agaaatggaa ttggtagtat aatggtggca cacatgaacg ttttggcatt agataataca
     5041 ccaaatttgc catcaacatt atctaaaaaa attgttcaag aaaaattgat tgatgaaatg
     5101 ggctttaagg gattgatttt tacagatgca ctaaatatga aaggtgtttc cgcaaattat
     5161 ccttcaggtg tagcagaagt aaaagcaata caagccggaa atgatgtgat gctttttcct
     5221 gagaatgtgg acaaggcgat agcgggaatc aaagctgcaa ttgatagttg tgaattgact
     5281 gaagagcgaa ttaacgaaag tgttagaaag gtattattaa ctaaatattg ggtaggattg
     5341 aataattttc agcctattaa aatgtctaat attgttgaag atttgaataa tgagaaggca
     5401 aatcttcttt tgacgaaatt ggtagaacat tctattacag ttgttgaaga taagaatgat
     5461 tttataccaa taatagatgt tagagagaaa attgctcatg ttgccattgg tattggaagc
     5521 ccaaatgtat tttctagtag aattgatgat tacgctgcga tagaccattt ttatatttca
     5581 aaatctgaga gtcaattgaa atttaatcaa ttgatagaac aattgaaaag ctacgataaa
     5641 attattttag agattaatga catgagtcgt tttttagcca aaaattatgg attaacggat
     5701 gttgaaattg gagccattaa taaaatcaat caatcaaaaa agacgataaa tataattatg
     5761 ggttctcctt atagtttgca aaaattaaat ggttatggtt ctataattgt tgcctataat
     5821 gaagactctg ttacacaaga tattacagct caaattttat ttggagcgag ggcaggatct
     5881 ggaacattac caatcagtgt tgccaattat gaatttagta aaggtgtttg tactaatgga
     5941 agccttaggc ttggatatgg ctttccgatt gaagttggta tgaatgctga aatattaaaa
     6001 ggtattgata aaattgccaa tgaagctata gagaatggag caacaccaag ttgtcaaata
     6061 ttagtagcaa aagatggaaa agttgtttat cgtaaatctt ttgggacaat gacctataag
     6121 gatagcaaat tggtagataa taatgattta tatgacattg cttcaatcac taaagttgtt
     6181 gcatctacac caattattat gcggatgtac gaagataaaa agctagacat caataaaact
     6241 ttaggaactt attataatta tccagaaggt agtaacaaga caaatctgac aataaaaaat
     6301 atattgttac acgaagctgg tttagttgct tggaagccat tttataaaag ccttttggat
     6361 tctgtaaatc aaataaaacc tgagtggatg agtaaaactt attctcctat gtttacaaga
     6421 aaagtaattg atagtgtatt tataagaaat gactttattg attccataag aaatacaatt
     6481 tacttttctg agttaggacc aaaagtttat aaatacagtg atttgggtta ttatttattg
     6541 aaagaaataa ttgaaaaaac ttatgctgat tctttgcaaa atgttgctaa aagatattta
     6601 tggagtttct taggtatgaa taatacgatg tataatccat tagcggctca agtaaatctg
     6661 aataatattg taccaactga agatgaaaaa ggatttcgta atactttaat tcacggatat
     6721 gtacacgacc aaggtgcggc tttgatgggt ggaattgctg gacatgctgg tgtatttagt
     6781 aatacaaatg atttagcaaa atactttcaa atgttagtaa atggtggcac ttatggtggc
     6841 acaagatttt acaataaaaa gacgattgat tattttactt ctaagcaatc ttcaataagt
     6901 agaagaggaa ttggttttga cagacctgag ccaagtggtg gtggaccgac ttgtagtagt
     6961 gcatcttctt tatcgtttgg acatactgga tttactggaa caattgcttg ggcagatcca
     7021 aaatatgatt tagtttatat tttattatcc aatagaataa atccgagtgc tgagaataga
     7081 aagtttatca atcagaacta tagaactaga attcaacaag taatctataa ttctattcaa
     7141 caataaactg ttttatagga aaagttatga atgattattt acgaatcttc tgagaaataa
     7201 ctataaagat agaacttcca aataataata atgttactgc aaataaggct tcattaatat
     7261 gtggaattgg tagataaatt aatccagcag caccttgaat ccctaatacc aattcattta
     7321 aaacaacgcc aactaaaaat gcgattgtag caaattttgt tgaatttgaa tttgaaagaa
     7381 agccattata aagtgagtac ccaattataa ataaactata tacacctaat aaaatcaaat
     7441 gtaaataggc tataataatt gatctaaaac caaagacaaa atgactaagt gatggaatta
     7501 tagatatcgt ttgaagtata aatttcacag tcattgcaaa ggttgcagca tacataaaaa
     7561 tatgcaccca agaaggtttt ttgtcgttta ttaaaagtgg aatgtatttt tttataaaat
     7621 ataaccaagc tatcatttga ataatagttg taattacggt aatgatatat agccacaaag
     7681 gtaatttgaa ccatagaatg gataaaaaga aagttggaat tactgtggca gcaaatacct
     7741 taaagtattg atttaggttg ggaaagtttt tagggagtat ggttgttaac atcgccatac
     7801 ttgcaaagaa gaaccagcca ttatattgaa aatgcaaaaa ataatattta taaatgaggt
     7861 acttatcttg ggtaaatgtt tttgaaacca tcatataagc caataaaaat ggaccaacaa
     7921 cagagagaat attaagaagt aatccagtaa ttgcccaaga actaaataaa ttatctttcg
     7981 gaattaattt attatctctg atgtaaaaaa cggcaaatag aatagctatg attaaagttg
     8041 ataaagcaga aaatataata gtatcagttt tagaaccaat gaatgtaaac aacaaagcag
     8101 caatatatgc acagattaaa ttaaatacaa tcagccattt gtactttttt tgatttgcag
     8161 aacttaagaa tggaaaaata agataagcta agccacaata taaagcatgg tttatccaac
     8221 cactgaaggc aaagttaaaa tgtgcgtaga gcaagttttt ttgttcaaaa aatggaaagc
     8281 taaaagctat tttatatcgc ataagtgtgc cataaaatgc aacaacaact aagcttaaaa
     8341 atccccacca aaaccaattt ttaaagattg ttttaatttc tttttccatc ttagaaatat
     8401 actttatgtt gtactatttt tactttttgt tgctcactca tttttctaag tgttcgaata
     8461 acggtttcta ctctcaaact tgtgaaatca gcaatttgct gacgtgtata aggaattaga
     8521 attttttctt caggcttatc tttttcctga ttttgttttt tatatctatt tagaaatagt
     8581 tcgattttaa tttcaggtgt tggagctgtc catatttgtg aaagcaatat attgtcataa
     8641 atttcttcag ctaatgaatg tagtagttgt tgattgatat cagggaagtc ttttaagatg
     8701 ttattaaagt tttctttagg aattctaaca aaaacaccag cacttaatgc ttgagcactt
     8761 actaaatgag gtttatttaa gagcaattca gattcgccaa agccttctcc ttctgaaaca
     8821 atgttatgtg taagaaattt gtcttccgtg ttagaagaaa aaatccttac ctcgccttta
     8881 attacttggt aataaaagag aggtaaggaa tcttgtttga aaataaagtc tcctttattt
     8941 attcgtttgg cgataccacc ataagcaata agtatgttat agtcaatatg cattaaaata
     9001 tttttatctt cttctgattt tattcaaatt taagattata tatccaaaat attgtaataa
     9061 aaatcgctct tatctattat ttaacacata gagttgatat aacaattttt tttcttattc
     9121 tacgataaaa tgtcctttca tcatagcaga gtgtccaggg aaagaacata agaattcata
     9181 agttcctttt gcaggagctg ggaattctac ttctgttgtt tctccaccac caattgtttt
     9241 agtgtgtgca attacatcac cagctttgtc agcaggaata taatctgttg cttgagcagc
     9301 cattgcagct tcagcaaatt ttgccaaatc aacaccttgc tttaataata caaaattgtg
     9361 tcccatagca acaacaggag cagtacctgt atgtactaaa gttaatttca cagtttgacc
     9421 ttcttttact ttaatttcag ttaagtcata cttcatatca tcgcctgcac ttagctcaac
     9481 ttcagctaca tttgcgtctt cagcaggtgc tgtttcttca gtagttggtg ttgtgttttc
     9541 agttgttgat gatgaattgt tgcatgctgt aaataagaaa gcagctgcag tcattgttaa
     9601 gaatactttt ttcattttaa ttaaatttat tgagtgaata ataaagtttt aagcgttgag
     9661 ttgtttctct aataaaatag atttcggaaa caaaatatta ttttctaagt ggatatgcaa
     9721 atgtaaatca tcttcaaatt cttttaataa agataatgta acacgataag tattgcaacc
     9781 atcttctggt actgtataat tattagttag tgcagaaatt tttctatatc gatcaccttc
     9841 ggtatcgtgt tcgtgcatca tttgttcaat aggattttgt actgttccaa aagcggcttc
     9901 gacagtcgtt tttgcagctt cagcttttac catttttctg atataaggaa ataaaattaa
     9961 ctcttctttt ttcatgtgtt tcgttaattc acctgctcct tcattaaaga gtgccaaaac
    10021 ttcttctaat tcagcatggc gaccgccgtg aactcttgta attttttcta aataaggcaa
    10081 tatttcagga attgcttttt ctacataacg atggtgtttt ttctcaacat aatctgctaa
    10141 taaatctaaa ggccaagaat tataatcaat atttgcagca ccaccttgtc ccattgttgc
    10201 tgttaattca ttaattaatt ggtctgcatc taaattttgg ttattacaaa cttcttggat
    10261 actacgattg ccattgcaac aaaaatctat tgctttgtct ttaaatacag cagcaactct
    10321 ataatcctga gctactaatt ctgctattgt gctttctttt gtgatattca tatctatttt
    10381 tcttaatact ttctaaaatt aggctaatgg aataacatca ttattcctat aatattgaat
    10441 tttgtacaag aacatttctg ccattctatt gccttgccat tttgcacgct cggcacgctc
    10501 tccaataaat atactatcta cagtgccata aaaaagcttt agccatcttt caaaatgtat
    10561 agcatcgaca ggaagttgtg cgtgaggtgg aaatggagct ccaaaatatg ttcgctcatc
    10621 atctaataat acagtttgcc aaaagcggta cattttctct aaatgttgtg gccaacgatt
    10681 ttggataaca ttattgaaaa cgtcttttaa aatttcatct tctctaactt taccataaaa
    10741 agtatctacc aaaagtttta catcttcaat gttttctatt tgttttattg tactcatctt
    10801 tattaaccta attatctaaa cggctgtacg aataaaatgt ttttgtctta ttttacgata
    10861 ttattaacct tttttccaat aattatttgc ttgtgcaata gtctggaatt caatagctac
    10921 aacatttgca gatgcaatta gttgtgctgt atctgacgca taatttactc ttagtttttt
    10981 tctttcttca gggtctgttt gaatagctgc aattgttttt cttgacattt ttattgctaa
    11041 ttctctacct tcttgaggtg ttgctgtaat taatgattct aaccaaattc ccatattgta
    11101 atctatttta atgatgaatg aattatctta ctaatattga ttttcctgtt ttgatttttg
    11161 ttgctaaatc gaaaattgtt gtggttgtta gcattgtttt taattctcct ctaactttaa
    11221 taaattggtc atgcattgga caaggtgcat caggattgca ttcgcttaaa cctaaagcac
    11281 aaccattgta aatagaatcg ccatcaattg caaatacaat ttcactaatt tttatttgtt
    11341 tcatcttatc taatggaatt tcaaatccac cagttggacc ttttaatgaa cgaacaatat
    11401 tttgtttggt taaagttcca aggattttgg cagtaaacgc ttcgggtaag tctgcatttt
    11461 cggcaacatc actaattttt acacgctttc cttctaaaga ttcagtcgcg atataagtta
    11521 atgcccgtat gccgtattca caagattttg agaacatttt attttaaatt ctatgcaaag
    11581 ataagcaaaa ttttaatttc ggataatatt gtcttaaata aaagtcaata tttaaggttt
    11641 cttaatatat cgctctattt tcccttgcct tgcaagacgg tactcacggt atagaataat
    11701 atttttaaat ctaaactgat gctcatgttc tcaatatata aaatatcata tctcattctt
    11761 tgtatcattt ctggaacatt gtgtgcataa ccaaatttga ccattccaag agatgtgata
    11821 ccaggtttga tacgttggag caattgatat tttggggtgg tttttgaaat tttatcaata
    11881 aaatatcggc gttctgggcg tggaccaacg atactcatat cgcctttcag tacattaaat
    11941 aattgaggca gttcatcaat tctatatttt cggataaagg aaccaatttt tgtaattctt
    12001 aaatcatttt cttgcgtaag ttgaggcata tcaccttccg cattttctat catacttcta
    12061 aatttataga tattaaatgg ttgactattt ttgcctatcc tttcttgagt ataaagaatt
    12121 ttgccagtgg atgttttttt gatgataata gcgataatac ataaaaatgg acttaaaaga
    12181 atcaatacta aaaatgataa cactatatca aaaagacgtt ttgtaactct ttgaaacgga
    12241 gttagtacaa aaggtgatat ttctataaaa cctgattcaa atatactttg tgttttatat
    12301 tttccagata gaatatcaaa atcattcgct tttattttaa caagaattcc tttggctaga
    12361 cattctataa ttagatgttc cacttgctga tgttcgtatt gatgagctat aataatcgct
    12421 tcttcatact gattttgatg aattagtgtg tgtgcatttt caaaatcaac ttcatttttt
    12481 atataatgac ttaaaatagg cagagattgt gtggaaagta tagatttaat ttgattcctg
    12541 tcttttgcct cgccaactaa aagtgtttca atttgtactt ttttatggat aattctgttt
    12601 tttgcataat ttaaaatgag caacctccca gttaataaag tgagcaagtg tatggataca
    12661 taagctgtaa acaataaata ataatcttga taatctctaa ttctatcatt tagcatgact
    12721 aaaaaatata gaaagaaagt gccaataatt gattggaaaa tagacttccc aacttcattt
    12781 aatcttgatt ttttataaat atcagtataa gcgttagaag taaagtaaca aatccaccaa
    12841 aaaacaggaa taaataataa accttttaaa aaattggtat cattggttaa aaatgatttt
    12901 tggaaatgga gttcctcaat atatatttta cgaaaactaa aaaagataat ccacgtaata
    12961 caggctgcaa tataatcaga cagtacataa aaacgaatat ctttaattct gttattcaac
    13021 tgtccaaaat ttatctcttt ttcaagccaa ggttctccat tttttcttct aattgctttt
    13081 tagttgcacc aatttgttcg gctgttttgc taatattcca atcgttttta tccaaatggt
    13141 gttcaataaa actacgttca gtatagtctt gaatttgttg caaatttgca aatcggtcaa
    13201 agtctattcc tgcttgttct atttttttat taggtccaga atagtccaaa acatcttgtt
    13261 tggtaatgct tttgccactc ataattatca aacgttctac cacatttcga agttcacgaa
    13321 tattaccagt ccagtttcta gcttgtaatg cttctactgc atctttttca atttttttgg
    13381 taggcacacc atattcgcca caaatttctt gaaggaaatg ttcagacaac attggaatat
    13441 catcttttct ttgatttaaa gatggaacat gaattaaaat gacgctcaaa cggtggtata
    13501 aatccaaacg gaatctgttt tcctcagctt cttttaataa atctttatta gttgcagcaa
    13561 caatacgaac atctactgta aaatctttgt cgccacccac acgagtaatt ttgttttctt
    13621 gtaaagcacg taatactttt gcttgtgccg aaaggctcat gtctccaatt tcatctaaga
    13681 ataaagtgcc gccattggct tgttcaaatt ttccgatacg ttgtttgata gctgaagtaa
    13741 atgcaccttt ttcgtgtcca aataattcac tttctatcaa ttcagatgga attgcagcac
    13801 aatttacttc aatcaaaggc ccattcgctc tattgctttg ttcgtgcaac catcgtgcaa
    13861 ccaattcttt ccctgaacca ttgtcgcccg taattaaaac acgagcatca gttggtgcaa
    13921 ctttctcaat tgtatctttt attttttgga ttgagttgga tgttcctaca atatcttttg
    13981 ttttagcaat tttctttttt agtattttag tttcagaaac taaattggat ttatccattg
    14041 cattacgcac ggtaatcaat aaacgattca aatcaggtgg ttttggaata taatcaaaag
    14101 cccctttttt tacagcttca acggcagttt caatatttcc gtgtccagaa agtacaataa
    14161 ctggcgtatc aataccttta ttttgtaatt ctgccaatac ttcaatgcca tctttttttg
    14221 gcattttaac atctaaaatt actacatcgt aattgccttt ttcaatcttt tgtacgccaa
    14281 tttctccatc ttcagcttca tcaatttcat atttctcgta ttcgagaatg tctctcaaag
    14341 ttcttctgat ggctttttca tcatctacta ttagtatttt aggcatattt atctttattt
    14401 atttaccatt gtaaagctaa gaaaaaatta gataatagat taaattgtat ttttataaaa
    14461 aaaagaatgg cgaaattaga tttaggtgct gtacaagaaa ctttattatt gcctttggct
    14521 gcgagggctt atgatgctga aagcaaatat ccaatactaa acgatcaaaa agctttggaa
    14581 ctttttaaac aattggtgcc aaatagaaag gcatttagaa aaaatttatt aaaagtcgga
    14641 atcgttagtt taacagagcg tggctggata atggataggg cagtgaaaga ttttttgaaa
    14701 agaaatccaa aaggaaaagt gttgaatatt ggtgcaggtt tagaaacctc tttttatcgc
    14761 ttaggacagc caaatgtgcc ttggtttgat ttagatttac ctgatagttt agcattaaga
    14821 aaacaattat tacccaataa ttttaaaaat gtaaaatata ttcctaagtc gatgtttgat
    14881 gaatcgtgga ttgacgatat aggtaatatt tctgatggtt tgatgataac agtgcctgga
    14941 gtttttcctt attttgatga aaaacaagtg caattatttt ttaatacttt tgctcccaag
    15001 ttaaaaggtg ctgaaattat atttgatgtg ctttctaatg caactagatt ttttgtacaa
    15061 cgaaaaatta accaagctgg aatgtccaaa gctactttgg attggggtat tatgaatcca
    15121 cggcatatcg aaaaatggaa tcaacatatt caatttgtaa aagcatatag ctatttgcaa
    15181 catccatctt ttaatgcgat tggaaatcca atttataatt ttatcagttg gaatagtaag
    15241 ttttttaaat ctgctcaaat atttcatttt aagtttgttt agtgcaaaaa aatggatgtt
    15301 tattttattc atatagataa ttttatttgg tcggaaacta tggagaatga tatagggtca
    15361 ttccctgaga atattcatca aaaaattaat gcttataagt ccatagaaca taaaattcac
    15421 gattattatg gaaaaaaaat cattcaattt ctattacaga aatatcaacc caattatgct
    15481 ttgtgtgatt tgaaatatac tgagaaaggt aaaccatatt ttgttggaga tttcgatatt
    15541 aatatttcgc attctggtaa ttttgtagtt ggtgtttatg tagaaaatgc tcaagtcggt
    15601 attgatattg aaaaacatag aaatattgag gccttggatt ttaaaaaata tttttcagaa
    15661 aatgaatgga atacaattca acattctgaa aatccactaa taactttttt tgatttttgg
    15721 gcaattaaag aatccgccat aaaatgcaat ggaaacggat ttaaggtatt gtctaaaaca
    15781 caaacttttg atgaaaaaag tatccaatgt gttgatgaaa tatataatta tcgtcaaata
    15841 acgattgata ataattatag ttgctcagtt tgttgtaaca acaaaattga aacgataaat
    15901 ttgatagaaa ttagtttata agattagtct atttgtacgg tttttacatc taataaattc
    15961 atcgcattgc taggtaagtt tttaattttt ttatgtgtaa accgtacaat ttggtcatag
    16021 aaaagtaaga taacaggtgc atcttctaac gccatttgtt ctaattgatg gtacattttt
    16081 aaagcatcaa ccgtattatt ggtttgcatt gctttttcat ataatttatc gtaagctgga
    16141 tttttataaa aagtataatt tggtggagct ccatatccac tataaaataa agctaaataa
    16201 ttttcagcat caggataatc gccaatccac gatgcacgaa ataattgtgc ttcattttta
    16261 cgcattgctt ctctcaaaaa tgcagctggt gaattttcaa tttttatttt gatacctatt
    16321 tctgccaatt ggtttgcgac actcgaaatt aaatcttgat acgtagggtt tgaataaact
    16381 gtaatttggt caataccttt gccatttgga taaccagctt caattaataa ttgtttagct
    16441 ttagaaaggt caaagtgata gcctttaaca acagtggaat caaactcatt catacctaaa
    16501 ggaataatgc cactttcggc agcaatacca atgccatttc tcaaataagt aatcattttt
    16561 tttcggtcta tggcataatt catagcttgt cttacttttt tgttttttaa agctgcacag
    16621 ggctgttttg ccattgaaat accaatgtat tcggtattca tataaggcat ctttaaaaaa
    16681 ttaattttgt ctttccattc tgactttagc actccttctt ttgttaatag tttgtctttg
    16741 aatgaaatat ctaagccagt cataaaatca agctcgcctt tttggaattg gttgtattct
    16801 gaatttctat tggcaataaa attaaatcgc acaccatcta tataaggtaa tctattgccg
    16861 ttgctatctt tttcaaaata atttttgttt ttatggacaa ttaatacatt gttttcttcc
    16921 caacgtacaa aataaaaagg gccagttcca actggatgct tacggaagtc tttaccgtaa
    16981 tgctcaacta cttcttttgg aacaacagaa caatattgta atgtaagcaa acccaacatc
    17041 ggtcgaaatg gttcttttaa atgaataaca aatgttgtgt catttatggc ttcaaatgga
    17101 ttttgttctt gtattttccc attaaataac caagcacctg ttgaggcaac agtggtatca
    17161 ataattctag ttaaagcata aacaaaatca gaagcaagca cttttctgcc ttttccgttt
    17221 ggaaacaatt cgtggtcgtg aaaaaacaca tcatttcgaa gtataaaagt ataatttaaa
    17281 ccatcatttg aaattgtcca agattttgca atcgaaggta aaatatttaa gttgctatca
    17341 atttgaacta aaccattata caattgatta gcacaccaca ttgtagcttg atcttttgaa
    17401 aatgctgggt ctaaagatgt taagccagcg gagatattaa gatgaaaaaa ttttgcagat
    17461 ttttctttgt gagtttcttt gcatgcaact aagcatagaa gacctatcca tactaaaact
    17521 aatgtattct ttttcatcta aggacaaata tacttgtatt gtgtagcaaa ataattttat
    17581 ttttagaaca cattgttagc ttaatttata aattaaatct tcccattctt gattaatttt
    17641 caaaaaatgc tttttgcata aatgtaaggg tgtttttgca atttcattac gctgaatgcc
    17701 tatcattaca ttaaattctt tctgaattaa agattgaaca gcagcaacac ctaagcgagc
    17761 tgccaaaatt ctatcttcag ctgttggatt gccgcctcgc aacaaatgtc ctaaaactaa
    17821 agttttgata tcatattcag ggtgtgtttt tttaaattca gctgcaatat tttcggcacc
    17881 tcctttgtca tcgccttctg aaactatgat gatgtaagac tctttatctg ttcctcgttt
    17941 taatctttct gacaaatcat ttaagttagt tttagtttca ggaataaaaa tatcttctgc
    18001 tccagttgca atgccagttg ctaaagcaat tgccccaaca tcacgcccca ttacttctat
    18061 aaaaaacaag cgtttaaatg cattggcagt atctctaatt ttatctacac attccattgc
    18121 tgtttgtacc gcagatttaa aaccaattgt gtattcagta ccaaataagt cgttatcaat
    18181 ggtgccagga attccaacaa aaggaatatt aaactcttgt gtaaattgaa tggcaccagc
    18241 aaaagtacca tcgccaccaa tcacaattac agcatcaatt tgatgttcta ctaagttatg
    18301 ataagctttg tttcttccct ctttagtctg aaaatctttg cttcgagctg attttaaaat
    18361 ggtaccgcct ctatgaataa tgttacctac acttttttta ttcagcggct gaatctgatt
    18421 gtccaccaaa ccttgatagc cattgtatat tccaaatact tttagttttt ctaataaagc
    18481 aattctgact actgcacgaa tagctgcatt catgccagga gcatcgccac cagaggttaa
    18541 aactgcaata tttttcatta taagtgttta atgttttcta aaacaaaacg aattaagctg
    18601 tctattgttt gttcagcatt tacagagaaa gtacctaatt gtctattggc aatcattgca
    18661 ttgaacgaaa tacaacgatg ccctaataat tgacctaaac cataaatacc agaagtttcc
    18721 atttcaaggt tagtgatttg taaatcgttt tcaaatttta aagcagatag tgtatcaata
    18781 aaatcttttt gtttgggttg caatcttaat tgtctgcctt gtggagcata aaacccagga
    18841 gctgtaagtg taattccacg tttaattcct tttgctaatt tatctaacaa atatgtatct
    18901 gcttgagtag tataagattc cgtattggta atttttgaca ctgcatcatt gattgcatta
    18961 tgagcattat gatattgata aaaatgcaat aagccttcta gtccaacggc atattctgat
    19021 actaaaaaac tatccattgg aatgtcattt tgcaatgctc cacttgttcc gatgcgtatg
    19081 atattcaacg attttaattg ctcatttggt gttcttgttc ttaaatcaat attgaataat
    19141 gcatctagtt cattcaatac aatatcaata ttgtcggtgc caattccagt cgcaataaca
    19201 gaaattcttt tgttgcctaa atatccagta tgtgttacaa attcacgatt ggctttctga
    19261 atttctattt tgtcaaaata tttggttacc atcggtacac gctcaggatc gccgacagta
    19321 atgatggtat ctgcaacatc ttcaggcaat aaagtaagat gataaacact accatcttta
    19381 tttaaaatta attcactttc tgcaattttc ataacttctt tttttgctat aataaactca
    19441 aattacaaat tgttgatgat ttgtaatgca ttgtctttgt tgttatacaa acttaaatat
    19501 ttaattcttt ctgtcaatat ttctgtcgta aattcttgtt gaaaatagtc ttttatttgt
    19561 tgttcgatat cattttcatt aacaaggata gttgcatttt gaagttcaga gccaataact
    19621 gctgcttgat ttgtaaagca aaaacgacct aaaaacaaag tattaattag cttcaatttt
    19681 acaccagttt caatctctga aaaaatgata ttgatatgtg cattttgaat tagcgtttct
    19741 aaatcttcgt gtgtcggatt ggaaattagt tgtatgtttt taaataattg aacagtttca
    19801 attagatgtt ccgttggatt ttttccagct ataattatag gaaattgaca atttttgagt
    19861 tggtttctaa gcaaacgtac aattggtaaa taattatctg tgatacttag gtttccatga
    19921 aataagatat attcgccttt cccaatttta gtttttattt cagtattttg gtgaaatgca
    19981 ggaataaatg tagtaggaat atttggaaaa cgattggtgt aataggcggt atcactggaa
    20041 gatatacaat gtaaggcttg gctgttttct aatatagatt ggtattttat aagttttttg
    20101 tattcaaaat aaaaatagat tttgtcttta atacctaatg ccaaatcaag caattttcca
    20161 taatagagcc attcaatatt gtgcagtcgt actatttttt ttctattttt taat
//
LOCUS       DAOQCW010000036        20192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_965_length_20192_cov_5.930129, whole genome shotgun sequence.
ACCESSION   DAOQCW010000036 DAOQCW010000000
VERSION     DAOQCW010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 20192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20192
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_965_length_20192_cov_5.930129"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..542)
                     /locus_tag="PLS16_04790"
     CDS             complement(111..542)
                     /locus_tag="PLS16_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 domain-containing protein"
                     /protein_id="HNJ60427.1"
                     /translation="MNFNLNRYIEAQEKQYAIALAEMQAGYKTSHWMWFIFPQISGLG
                     FSEVSKFYAIKNLEEAKAYLQHGILGKRLREISNVLLMQDTDDATLIFDNPDDMKLHS
                     SMTLFAIADEENLDNVFEKVLAKFFKGKYDRRTVEILTEQQ"
     gene            complement(677..1543)
                     /locus_tag="PLS16_04795"
     CDS             complement(677..1543)
                     /locus_tag="PLS16_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNJ60428.1"
                     /translation="MKHLKITSKITTRDSYSIDRYLSEISKLPLLSDNEEIELAQMVK
                     KGSTEAVNKIITANLRFVVSVAKQYQNQGMSLNDLINEGNIGLIKAASKFDETKGFKF
                     ISYAVWWIRQSILQALIEQPRIVRLPMNKAIVYNRIMNVIQDFEQKNQREPSNDELAE
                     LLDMRADELIILRRSLAFHSSVDVTIGEDDDTTVLDTMVGDTFEQPDNNLIRNSLDTE
                     IKHSFQILSDREQEVIKYYFGMNEYGQTYSLDEIGEKMDLTRERVRQIKDKAIRKMRK
                     NKCALELKTFLQ"
     gene            complement(1626..2378)
                     /locus_tag="PLS16_04800"
     CDS             complement(1626..2378)
                     /locus_tag="PLS16_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HNJ60429.1"
                     /translation="MRDCLIIIPTYNEKENIPKIIPYTLNLSERFEILVIEDGSPDGT
                     ADLVKSLMLTYPQRVHIIERRGKLGLGTAYITGFKWALERSEYKYIFEMDADFSHNPD
                     DLLKLLDACENKGAYLSVGSRYVKGGGVQNWSWDRLFLSSFASKYVRIITGMKVKDTT
                     AGFVCYRNETLQKIDLDKIVFIGYAFQIEMKFAVWLQNLPIIEVPILFKDRELGTSKM
                     SGSIIKEAVLGVISMKIDSLKNRYFLKRKRLA"
     gene            complement(2448..4109)
                     /locus_tag="PLS16_04805"
     CDS             complement(2448..4109)
                     /locus_tag="PLS16_04805"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HNJ60430.1"
                     /translation="MNRIYLVLFCIVCFGKSIAQTIDKSKLFPPFIETKAPEKKIQIQ
                     KTDSLIYENWEWGEYRKLIGNVQLKDSSAYMWCDSAILDITANYLTAYGNPLHLKNGD
                     SIDIWGNFLEYFGDQKQARLTGNAIMKDKKMVLTAPEIYYNTASDIGYYNTGGRMVKD
                     ETILTSQIAYYYHQNGEAVFYKNVVLNTKETTIVSDSMRYNINDEIVYFIAKTKITNK
                     DGNIIYTDKGSFDTKTEKATFDKNTAITKDNSIIKGDTINYDNKTKNAIAQGNVSFQD
                     TSENVTILSGHSLYIDKTQYVRATKDPLLINVSVNKEDTIQKQDTMFMSADTLIAYKI
                     AHQTFDEDSTIQIDSLKIMHAYHHTKMFRRNLSAVCDSLYYTQLNDVFKLYYNPILWI
                     DSLQLSGDSIYIYSENDKISHISVFGNAFIIHWVENEIFNQIKGKIINIFIRDNKIVL
                     MDVDGSSESIYFIKDDNKGYIGGNQSTSGNIAIYFKNGEIDRLKLKNAPEATFTPMKK
                     ISPPNYRLSGFNWQWQIKPKNKYDIIRNKKQYDDFVKSNSLFYEK"
     gene            complement(4149..5189)
                     /locus_tag="PLS16_04810"
     CDS             complement(4149..5189)
                     /locus_tag="PLS16_04810"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNJ60431.1"
                     /translation="MKHKNEFTVGALTIIGIIAFILGYKYLKGEDVFSSSKYIVCYAD
                     QTGGLLPSNQVLENGVGIGRVSEVTISRSPKYPNKAIFILKLDSDIDVPKDSKFAIVA
                     LDMLGKMGIALMRGTSTEYATEQDTLSCISIGNTLDQAVDLFADVKPKLDSLMTSVTG
                     MVNNLNNSLGSGENNLLTKAMTDLSGTLQSVNKLAKTLDNTFTEEKSNLHGILTNVNN
                     LTASFNKETGKIDSILTNFNTLSGKLAVMDLESTVASAKNTLEQLQTTLKKVNEGDGS
                     IAKLLNDDALYKDVLGTIKSLNDLLADLKKNPKKYISLSLIDKSKHITVDTPQDSLDI
                     VSKKKVKIIDNK"
     gene            complement(5222..6454)
                     /locus_tag="PLS16_04815"
     CDS             complement(5222..6454)
                     /locus_tag="PLS16_04815"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNJ60432.1"
                     /translation="MNTKNNVITSIIKSSLLFICIFFLQSQYAYAEFKFKTIVLDAGH
                     GGHDAGCKGYSGHSHEKDVTLAIVLKIGKLIEQNYPNIKVVYTRKTDVFIPLEERAAI
                     ANKNKADLFVSIHCNANVNTSAYGSETFLMGLHKTEANLDVAIRENSVIKLEKDYKKT
                     YDGFDPDSPESLIALSLAQNANIEQSSFLAGRVQNYFANDLKRFNRGVKQAGFWVLYR
                     TTCPSILIETGFLTNPKEEKFLTSENGQNDMAESVYHAFLDYKAYIEKNPIKTNPIVV
                     KPEPINVPTKEDTEEIKPIIEAKTDTDNVKITPPIESPNSSNNVASSNGIEYKIQLTA
                     GSTLFPQSHKIYHSDVKVHYEKIDGLYKYLTEPIDNYANAILMQAKVRKIGYSDAFIT
                     VYKDGVRLSLAESKKYLK"
     gene            6607..9216
                     /locus_tag="PLS16_04820"
     CDS             6607..9216
                     /locus_tag="PLS16_04820"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HNJ60433.1"
                     /translation="MDTLLQNENSTNVEVQTYKIADDQLEDIVKYKAKDSIIYSISDK
                     TMYLYGEANISYQEMQMWATQIRYNWDNGEVTALGGMNDTTGYYDKVYFTQAGENFEA
                     DSARFNFKTKKGKSFGLVTKQLDGFLHSETVKVMNEDMFYAKNARYTTCDLDHPHFYV
                     EIAKAKVVKDKFIVGKPADLVIADVRTPLFLPFAFIPNINKKEKQGSGLIFPTYGDQQ
                     DLGFFLKGLGYYHKINEKMDISTTVDIYTLGSWAINLTSSYKKLYKLYGNFNMNVGQV
                     RRGFANEKRNPNYTKPPIDFSVNWNLNLDPKRLYNATFGLSLNVVSSRRYQQLSNQDP
                     LKVVSNTFTSSISYNKTFPGKPFSLGISSNYVQNTETKAVTLTLPNFNFAVTRINPFE
                     KKIRSTSRKWYEEIGFSYSLNAVNNINTYDSIFFKRETLNRMKNGIVQNLPISANFRL
                     FKFLNFNTTFNYTERWHFYYTDRVFRDTLTYFDTRDSVYKLRRNVTEIDTTYKFNTNR
                     NFGLNLGLSTNLYGTFQFKKSKLMAIRHTFRPSMSFNFTPDFSKPLWNAWRTVQTDTS
                     GRTTDYSRYEYTQFGGPSKGKNASINFNFSNTLEIKIKSKKDTITGTRKITLLDALNF
                     GVGYNFAAEKFKLNFSGISGSTHITDKLNMNFRVGMDPYALDTNGVRINEFYFKHKKR
                     FFRFTGMDISLNGSYTSKKYNNSGTMRMQQEQGLDLSPTFRNIYQLGYYNFDIPWSIN
                     YNYSLNWSKSFVNKKDTNIITQAIGIGLDFNITSKWKINVNTGFDLTNKRVTRTDISV
                     VRDLHCWQLEMRWSPIAAQQSFFITIYVKSQQFNFLRLQKQKSFFDSGFFGSGGAGMG
                     GLGNMMGGVANTF"
     gene            9251..9403
                     /locus_tag="PLS16_04825"
     CDS             9251..9403
                     /locus_tag="PLS16_04825"
                     /inference="COORDINATES: protein motif:HMM:NF039953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HNJ60434.1"
                     /translation="MLELIKDLSQFLWERKQWWLFPLIISLILVALLIVFGGSTAVTP
                     FVYSIF"
     gene            9801..11105
                     /gene="ffh"
                     /locus_tag="PLS16_04830"
     CDS             9801..11105
                     /gene="ffh"
                     /locus_tag="PLS16_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNJ60435.1"
                     /translation="MFENLQDKLEGAFKNLKGQGKITDINIATTVKEIRRALVDADVN
                     YKIAKEFTDTIKDKALGQNVLTAVSPGQLMVKIVSDELTELMGGTVTELNVKENPTII
                     LISGLQGSGKTTFSGKLSYYLKNKLKKSPLLVACDVYRPAAIEQLKVLGEQNKVEVYA
                     EIENKNPVEIAQNAINYAKQKGYNVIIIDTAGRLAVDEQMMQEIYDVKTAIKPTETLF
                     VVDSMTGQDAVNTAKAFNDRINFDGVVLTKLDGDTRGGAALSIKYTVNKPIKFVSRGE
                     KMETLDVFFPDRMAQRILGMGDIVSFVEKAQEQFDEKEAQKLSKKIAKNQFDFDDFLQ
                     QINQIRKMGNLKDLLGMIPGVGKAMKDIDIDDNAFKKIEAMIFSMTPHERANPDVLNG
                     KRRERIAKGSGSSIQDVNNFVKQFDQMKVMMKRFQGMGMKQK"
     gene            complement(11119..12396)
                     /locus_tag="PLS16_04835"
     CDS             complement(11119..12396)
                     /locus_tag="PLS16_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNJ60436.1"
                     /translation="MSIDSVLSRMNKKDSFVLIIYAVTIGLFASLLAISLMGITEYFE
                     HQIFHSAKQYHPLVYIFLPTIGITSIYYLRKYVFHNKKNKGISEIYNTLDYPKDHLPL
                     FKLPSHYINGFLTVIFGGSTGIEVSTVVATATLGNTVYKKDFGARAYKRELVSAGVAA
                     GIAILFNSPIAGWLFAWEVIAKKMNRAFVLCCTIATFVAWIFLQILHPEPILPIAVTG
                     WNWYAIPFMIVLSLFGGSLSIYFTILVLRIKGYFSGINNNFIRVNLGALAVGTMICFF
                     PFIYGDSYHALEEMIGGYHNYSILFLLFIAFLKPLASSLTLGAGGDGGVFAPSIVAGA
                     FLGVAFALICNVYLGTSLQLLNFALIGAATTLSAAIYAPFTSLFLICNLVPNGYELFF
                     PLLICCLIANFFAKLIFPYNVYTYDLDKKVSNH"
     gene            complement(12546..13202)
                     /gene="mnmD"
                     /locus_tag="PLS16_04840"
     CDS             complement(12546..13202)
                     /gene="mnmD"
                     /locus_tag="PLS16_04840"
                     /inference="COORDINATES: protein motif:HMM:NF033855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HNJ60437.1"
                     /translation="MDIDLVITNDGSPTLYVERFDEHYHSIHGSMQESLHVFIQAGLD
                     YKAKQQPSIRIFEMGLGTGLNALLTLNYAQQHNVFVDYHAIELYPLTLESINKLVQFP
                     NFNHPNFLELHQIKSNKMLNIGENFRFTKYLQDINDYKPSQLFDLIYYDAFAPSAQDE
                     LWTVEMFQKMYDMLDDGGCLVTYCAKGIVKRTLKSVGFNIESLPGPIGKREMTRAIKL
                     "
     gene            complement(13208..18391)
                     /locus_tag="PLS16_04845"
     CDS             complement(13208..18391)
                     /locus_tag="PLS16_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNJ60438.1"
                     /translation="MKRNLILLLLCTFYVHTLLFSAIQLVEIQPSDNKKLDNHLFVSE
                     DIAYIVVSESIAKSYAKNIETYIGNHQYLINIPKEKVNSIASKIYYVQPESKVFDDID
                     KTATIQNIQVQFASTIKKVNAIEILKSLNVSLYDFIEDDHIYKLQITTSKIAELAQLP
                     FVLLISKDYKDKQLLVSDSYIMDRLPSVNMPISKGGLNLNGENMNIGIWDGGAIGTHI
                     NLEDTRVFNIDKTLSFSPYNVHPTQVAGAAAMCGNTSYLHSGAASKSNIYAWDNWGNI
                     IQEIKDGISKYQLNVTNHSYNFEQSHCKSASGLYTLDASKMDKLAYEYPNVLHVVASG
                     NTAHLCAINDTFYSLDIGYQGTKNAITVGYLYRNEKLVETSGRGPTTDGRLKPELVAK
                     GFAYTASAPGTAFSTVYGSSFSAPQIAGIATLVQQKYKLQNGVLPPSSLVKAVLCNTA
                     EDMYRKGLDYGAGFGRPDAKKAVQNIIDNKFIIGNISNQNQNNHQFTLTQNADFVSIT
                     LCWTDPAAYPLQHKILVNNVDLKLITPNNDTILPWVLNPIFYQNPAQRGIDNLNNIEY
                     VTLDSALAGTYTILVNGTDIVTDQQQYSISYTFENNGIDIVYPDGGETLDAGANFNVT
                     WNTRNVEDSLSFYYSIDSGINWTFIKNVHSRDAFFTWTTPNINTKNALIKAINSQFSD
                     SSNATFNINFRPNANFITIAPCDKSANIKWPAIPNVQYYTIYVFDDFVWRKVDTTSKL
                     SYQLTGLVNGKSYVVEMTATVDGVESNKSTAKIFTPSFTACSQLNDIGVLTVINPMGG
                     RMHTSSQLGNQVKPVFVIKNYGSNPISNFNLSYQVNEGTIYTSTISRTINSNDTIQVQ
                     ASNDYDMSLAGNYNLKAYSSFSLDTLPNNDTLVYTLKHLANEPISLPWTESFENSGAT
                     YSTNIFGIDGMEYADFNTDKYGRWRIRDNMYALSGKRALTLDNYSNTGSIKSSHIITL
                     NLANYIDSIIYLDLSFMQHGETATDNIVYARGNDTATWIPILNLFQNKAPKGEYNRVQ
                     AINLFNLLYVDNGQTVSSSFQIKLATTNTKASNELTKSGGYTFDDIKLYVANNDLANS
                     TEGKDSILCLNESKNIPIKIKILNQSNQARSNLKVTYIVDNANVVSETISDIILPYQT
                     FDYTFNQTLAITQSGKYVVHVIADDVDDKYKGNDTSDAISFIANLYIDSLPYLNNFEQ
                     TSLCIPEGQNSSWAWSTPNKTYTKMAAQGNKAWTNSTFDTYNYSENSSLYLGCYNMQS
                     LKNTPSVAFNHLLDIENLYDSAWAEYSIDGNEWNRIGCANCGTNFYGNMNGAAKWDKT
                     LMPWQVASSKINIDTTLNNQHVFIRVRLQSDEGIVGEGLAIDDWHVYVTNYTIPLNDS
                     VFIDTISNGNGWIPILKDDKIIAEIYDDNKVLGNITVGYFASNQVRKYQGKALLPRNW
                     SINTENQVDGNFKIRLYLTNEEYLMLLNTDSQLFSMRDIAALRYNGFNQDVVFENNYI
                     KGDFVSIQTDSLYFVPFENGYYVEMNTQKFGEFYLTSKAINPTFVPTTQIVTLDADQM
                     GDDAILAWNTTGDTNVLKYNIQYSIDGQHFQEIASIYSLHNISDTSDYYFIHNSNAAI
                     GTVLYYRIEAVLNSGVSIQSLVDSIQFENMTGIKKNVLQIKSYFSQNQLHIEHNNSIL
                     GNYNLVLVQLDGRVVFQKNIQIDSNKNSIDIPIQLLSNNIYILGLQNKNKIYYSKLYK
                     N"
     gene            complement(18462..18674)
                     /locus_tag="PLS16_04850"
     CDS             complement(18462..18674)
                     /locus_tag="PLS16_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60439.1"
                     /translation="MSDIFTPDFLLKFIYNETNTLENEMIVNEMKIDTDLEQLYHELL
                     ESIAILDTLKFSNIACTSNNILELKV"
     gene            complement(18759..19700)
                     /locus_tag="PLS16_04855"
     CDS             complement(18759..19700)
                     /locus_tag="PLS16_04855"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ60440.1"
                     /translation="MFKKLLFFFLILLLFVGIYVFFLRKDLTLSREYIKEKYKEPNSK
                     FIQWKGAEIHYTESGKGFPILMIHGFGGSFKDFEKLDHLLNPNYKVIRIDVPGFGLSE
                     FPGKPTPQTDFNSIYNEYFNFLIDTLHLDSFYVMGNSLGGMMSWVLTLEHPDKVKKLV
                     LLNSAGYDMKEVEKSAGVKRFQNPIITAFLKRGVPYFMTENGVNRVFYHKSNLSAEKT
                     QRMNDFWNRKGALEQIICMSNSKNYPDQNKIKNIQVPTLILWGKEDIIINSKFAERFH
                     QDIKNSTMIVYDSCGHVPMAEYPEKVLNDVNAFFDKQ"
     gene            19820..>20192
                     /locus_tag="PLS16_04860"
     CDS             19820..>20192
                     /locus_tag="PLS16_04860"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HNJ60441.1"
                     /translation="MAISIFDTLIKKAEALIASDGKVSQLLDEFFLMVGKSTEKLYKI
                     QDSVVTLGRLLRAWVKGDYKNISTSSIIAAVAAIIYFVNPLDLIPDFIPIIGQIDDIL
                     VLGYFVKILNKEIEKFMAWEAA"
BASE COUNT         7028 a         3165 c         2824 g         7175 t
ORIGIN      
        1 agtcccgtcc ggaccgcaaa aaaagtcctc aaatttttga ggactttttt tatttaaatt
       61 actctatgga attcctacta aatttgagcc taaaaatgtc cattaagact ttattgctgt
      121 tctgtcaaaa tctctaccgt ccttctgtca tacttccctt taaaaaattt cgctagtact
      181 ttttcaaaca cattatctag attttcttca tctgcaatgg caaataaagt catgctggaa
      241 tgcagtttca tatcatctgg attgtcaaaa atcaaagtgg catcatcggt atcttgcata
      301 agcaacacat tagatatttc tctcaaccgc ttacctaaaa ttccatgttg taaatatgct
      361 ttcgcttctt caagattttt tatagcataa aactttgaga cttcactaaa acccaatcca
      421 gaaatctgag gaaagataaa ccacatccaa tggctagttt tataacctgc ctgcatttca
      481 gctaaagcaa tggcatattg tttttcctga gcttctatgt atcggtttaa attaaaattc
      541 atggctactc atacttattc taaaaaataa atagctatta cggaatatta tcaatggaat
      601 tccaactaaa ttcgggatta aacttttcgg acagcctctt tttattattt ttctatgttg
      661 catttagaaa actctgttat tgtaaaaatg ttttcagttc taatgcacat ttattttttc
      721 tcattttacg gatggctttg tctttaattt gtcgcactcg ctctctcgtt aaatccattt
      781 tttcaccaat ttcatctaag ctatatgtct gtccatattc attcatacca aaataatatt
      841 ttatcacttc ttgctctcta tcagatagga tttggaatga gtgttttatc tctgtatcca
      901 aagagtttct aattaaatta ttatcaggtt gctcaaatgt atcacctacc attgtatcca
      961 aaactgtggt atcatcatct tctccaatcg taacatcaac ggaagaatga aaagccaaag
     1021 aacgtcgaag aataattaac tcatctgctc tcatatctaa taattcagcc aattcatcat
     1081 ttgaaggttc tctttggttt ttttgttcaa aatcttggat tacattcata attctattgt
     1141 acacaatcgc tttattcatt ggtaagcgta caattctagg ttgctctatt aatgcttgca
     1201 aaatagactg acgaatccac caaacagcat aagaaataaa cttaaatcct ttggtttcat
     1261 caaactttga agctgcttta atcaacccaa tattgccttc attaattaaa tcatttaaag
     1321 acattccttg attttgatat tgtttagcaa ctgataccac aaagcgtaaa ttggctgtaa
     1381 taattttatt gacagcttct gtacttccct tttttaccat ttgtgctaac tcaatttcct
     1441 cattgtccga caataaaggt aattttgaaa tttcagacaa gtatctatca attgaataac
     1501 tgtctctagt agtaatttta gatgtaattt ttaagtgttt cataggcaaa gttttagttt
     1561 aaaaaatatc tttccctatt tatacgtaaa taatctaaaa atgttacaaa actatttata
     1621 aattattaag caagtctttt gcgttttaga aaatatctat ttttaagact atctattttc
     1681 atagaaatta cacctaagac ggcctctttt ataatacttc ctgacatttt tgatgttcct
     1741 aattctctat ctttaaatag aataggtact tcaattattg gtaaattttg taaccacact
     1801 gcaaatttca tttcaatttg aaatgcataa ccaataaaaa ctattttatc taaatctatt
     1861 ttttgtagtg tttcatttct ataacaaaca aagcccgcag tggtatcttt aactttcatt
     1921 cctgtaatga tacgtacata ttttgaagca aatgaggata aaaataacct atcccaagac
     1981 caattttgaa cgcctccgcc ttttacataa cgagaaccaa ctgataagta agcaccttta
     2041 ttttcacaag catctaataa ttttaataaa tcatctggat tgtgcgaaaa atcggcatcc
     2101 atttcaaata tatatttata ttcacttcgt tccaaagccc acttaaagcc agtaatgtaa
     2161 gcagttccta aacctaattt gcctcttctt tctataatgt gtactctttg cggataagta
     2221 agcattaaag acttaaccaa gtcggctgtt ccatctggcg aaccgtcttc aatcactaat
     2281 atctcaaatc tttctgaaag atttagtgtg taaggaatta tcttggggat attttccttt
     2341 tcattgtaag ttggtataat aatcagacaa tcccgcaaag tgtaattttt aacaaaaata
     2401 tgttttatat tacaattaga aaattaaatt atggattttt aagcatatta cttttcataa
     2461 aaaagcgaat tgctttttac aaagtcatca tattgctttt tatttcggat aatatcgtat
     2521 ttatttttgg gttttatttg ccattgccaa ttaaaaccac tgagtctata atttggtgga
     2581 ctaatttttt tcatgggtgt aaatgttgcc tctggtgcat tttttaattt caatcggtca
     2641 atttcgccat ttttaaaata aatcgcaata tttcctgacg tagattggtt acctccaata
     2701 tatcctttat tatcatcttt tataaaatat atactctctg aactaccatc tacatccatc
     2761 aaaacaattt tattatccct aataaaaata ttaattattt tacctttaat ttgattgaat
     2821 atttcatttt ctacccaatg aataataaat gcatttccaa aaacggaaat atgacttatt
     2881 ttatcgttct ctgaataaat ataaatagaa tcaccgctta actgcaatga atctatccat
     2941 aaaatcgggt tatagtataa cttaaaaaca tcattcagtt gtgtataata taaggaatca
     3001 caaacggcgg ataaatttct acgaaacatt tttgtatgat gataggcatg cattattttg
     3061 agagaatcaa tttgtatagt actatcttca tcaaatgttt gatgtgcaat tttataagca
     3121 atcaaagtat cagcactcat aaacattgta tcttgctttt gaatggtatc ttctttattt
     3181 actgaaacat tgattaatag aggatctttt gttgcacgta catattgagt tttatcaata
     3241 tataaagaat gcccgctcag aatagtaaca ttttctgagg tatcttgaaa tgaaacatta
     3301 ccttgtgcaa tagcattctt tgttttattg tcataattaa tagtatcccc ttttataatg
     3361 ctattatcct tagtgatcgc tgtattttta tcaaaagtag ctttctccgt tttggtatca
     3421 aaacttcctt tatctgtata aattatattt ccgtctttgt tggtaatttt tgtttttgca
     3481 ataaaataaa caatttcatc attgatatta tatcgcatag aatctgacac gatagttgtt
     3541 tcttttgtat ttagtacaac atttttataa aaaacagctt ctccattttg atgataataa
     3601 taagcaattt gtgaagttag aatcgtctca tctttgacca ttcttccgcc agtattataa
     3661 tagccaatat cagaagcagt attataataa atctcaggtg ctgtcaaaac cattttctta
     3721 tctttcataa tggcatttcc agttaaacga gcttgctttt ggtcgccaaa atattctaaa
     3781 aaattacccc aaatatcaat agaatcgcca ttttttaggt gtaatggatt accgtaagct
     3841 gttaaataat tagctgtaat atctaaaata gcactatcac accacatata agcactgcta
     3901 tcttttaatt gtacatttcc aattagtttt cgatactctc cccattccca attttcataa
     3961 attaaagaat ctgttttttg aatttgaatc ttcttttcag gagctttagt ttcaataaat
     4021 ggtggaaata attttgactt atctattgtt tgagctatgc ttttgccaaa acatacaata
     4081 caaaataaaa caagatatat tctgttcatt tgggcaaatt cagcgattca atcaatatcg
     4141 aaaaatcatc acttattgtc gataatcttt acttttttct tagaaacaat atctaaagaa
     4201 tcttgtggtg tatcaacagt aatatgtttt gatttatcaa ttaaagaaag actgatatac
     4261 tttttaggat ttttcttcaa gtctgctaat aaatcattta aagatttaat agttcctaga
     4321 acatctttat acaatgcatc atcattcagc aatttagcaa tactaccatc accttcattt
     4381 acttttttaa gtgtagtttg taattgctct aaagtatttt ttgcagaagc aaccgtactt
     4441 tccaaatcca taaccgctaa ctttccagaa agcgtattaa agttggtaag aatactatca
     4501 atctttccag tttctttatt aaatgaagct gtcaaattat tgacatttgt tagaatgcca
     4561 tgtaagtttg atttttcttc tgtaaaagta ttgtctaaag ttttagctaa cttatttaca
     4621 gattgtaaag tccctgataa atcagtcata gctttcgtta ataaattatt ttctccacta
     4681 cctaaagaat tattcaaatt attgaccata cctgtaacag aagtcattaa agaatctaat
     4741 ttaggtttca catctgcaaa taaatccacc gcttgatcta gcgtgtttcc aatagaaata
     4801 caagatagtg tatcttgttc tgttgcatat tcggtgcttg ttccacgcat taaagcaata
     4861 cccatttttc ctaacatatc taaagcaaca atcgcaaatt tactatcttt aggtacatca
     4921 atatctgaat ctaattttaa aataaaaatt gctttattag gatatttagg gcttcgagaa
     4981 atagtaactt cagatacacg accaatacca acgccatttt ctaaaacttg atttgatggt
     5041 aataatccac ctgtttggtc tgcataacaa acaatatatt tacttgaaga aaatacatct
     5101 tctcctttta aatatttata ccctaaaata aaagctatta ttccaataat cgttaatgct
     5161 ccaacagtaa attcattctt atgtttcact ctaatttgtt atttctcaca aatataaaaa
     5221 attattttaa atattttttt gattctgcaa gagataaacg aacaccatct ttataaactg
     5281 tgataaatgc atctgaatag ccaatttttc tgactttagc ttgcatcaaa atggcatttg
     5341 cataattatc tattggctct gtaaggtatt tatataaacc atctattttt tcataatgta
     5401 cttttacatc tgaatgatag attttatgac tttgaggaaa caaagtagaa ccagcagtaa
     5461 gttgaatttt atattcaatt ccattagatg atgctacatt atttgaacta ttaggacttt
     5521 caattggtgg agtaattttt acattatccg tatctgtttt tgcttctata ataggcttaa
     5581 tttcttcagt atcttctttg gttggcacat tgattggttc tggttttaca acaatcggat
     5641 ttgttttgat aggatttttt tcaatatatg ctttataatc taaaaaagcg tgatatacag
     5701 attccgccat atcattttga ccattctctg aagttaaaaa tttttcttct tttggattgg
     5761 ttaagaaacc agtttcaatc aaaatagaag ggcaagttgt acggtataaa acccaaaatc
     5821 ctgcttgttt tacacctcta ttaaaacgtt ttaaatcatt cgcaaaataa ttttgtactc
     5881 gtcctgctaa aaatgaactt tgttcaatat ttgcattttg tgcaagtgaa agtgctatca
     5941 aactttcagg cgaatctggg tcaaaaccat cgtaagtttt cttataatct ttttccaatt
     6001 tgattacgga gttttcacga atcgcaacat ctaagttggc ttctgtttta tgcaaaccca
     6061 ttaaaaaggt ttcagaacca taagctgaag tgtttacatt tgcattacaa tgtatggaaa
     6121 caaataaatc agctttattt ttatttgcaa ttgctgctct ttcttctaat ggaataaata
     6181 catccgtttt tcgtgtataa acaaccttaa tatttgggta attttgctct ataagttttc
     6241 ctattttcaa aacaatagca agtgtaacat ctttttcatg tgaatgtccc gaatagcctt
     6301 tacaaccagc gtcatgtccg ccatgaccag catctagaac aatagtctta aacttaaatt
     6361 cagcataagc gtattggctt tgtaaaaaga atatacagat aaaaagtaag gaagatttta
     6421 taattgaagt aataacatta tttttggtgt tcataaaata actttttggg ttgtcaaaag
     6481 atatatcgtt tattcttatt tattagttgc attatatcgt ttggattagg cttcagtcaa
     6541 tcagacagca taaacaatgc caatcttata gatacgatac aatctattga tacaaatata
     6601 gtgagattgg atacattatt gcaaaatgag aattccacaa atgtggaagt tcagacctat
     6661 aaaattgcag atgaccaact agaagatatt gtaaaatata aagcaaagga ctcgattata
     6721 tactctatta gtgataaaac aatgtattta tatggagaag ccaatatttc atatcaagaa
     6781 atgcaaatgt gggcaacaca aattcgttat aattgggaca atggcgaagt tacagcactc
     6841 ggtggaatga atgatactac tggatattat gacaaagttt actttacaca agcaggagaa
     6901 aattttgagg cagattcagc acgatttaat tttaaaacta aaaagggaaa gagctttggt
     6961 ttagtaacca aacagttaga tggattttta cattctgaaa ctgtgaaagt aatgaatgaa
     7021 gatatgttct atgctaaaaa tgctcgctat acaacctgtg atttggacca cccacatttc
     7081 tatgttgaaa ttgcaaaagc taaagtcgta aaagataagt ttatcgtagg aaaaccagct
     7141 gaccttgtga ttgcagatgt tcggacacct ctttttttac cctttgcttt tattccaaat
     7201 atcaataaaa aagaaaagca aggctcaggt ttaatttttc caacttatgg agaccaacaa
     7261 gatttaggat ttttcttaaa aggtttaggt tattatcata aaatcaatga aaaaatggat
     7321 atatctacca ctgtagatat ttatacatta ggaagttggg caataaacct tacctcttct
     7381 tataaaaagt tatacaaatt atacggtaat tttaatatga atgttggaca agtacggaga
     7441 ggatttgcca acgaaaaacg aaatccaaac tatacaaaac ctcctattga ttttagtgta
     7501 aattggaact taaatctcga tccaaaacga ttgtataatg ccacatttgg attaagttta
     7561 aacgttgtta gtagcagacg ttatcagcaa ttaagcaacc aagatccatt gaaagtggta
     7621 tccaatactt ttacatcaag tattagttat aataaaacat ttccaggaaa accattctca
     7681 ttaggcattt caagcaatta tgttcagaat actgaaacta aagctgttac acttaccttg
     7741 ccaaatttta attttgcagt tactagaatt aatccttttg agaagaaaat aagaagtact
     7801 tcaagaaaat ggtatgaaga aattggcttt agttattcat taaatgcagt gaataatatc
     7861 aatacttatg attctatttt ctttaaaaga gaaacattaa acagaatgaa aaatggtatt
     7921 gttcaaaatt tacctatctc tgcaaatttt agattgttta aattcttaaa tttcaacact
     7981 acttttaatt atactgaacg ttggcatttt tattataccg acagagtatt tagagatact
     8041 ttgacgtatt ttgataccag agatagtgta tataaattaa gaagaaacgt taccgaaatt
     8101 gatacaactt ataagttcaa tacaaataga aactttggat taaatttagg tttaagtact
     8161 aatctttatg gaacttttca attcaaaaaa tctaaattga tggcaatacg gcacactttt
     8221 cgaccatcga tgagttttaa ttttacacca gactttagta agcctttatg gaatgcttgg
     8281 cgaaccgttc agactgatac atcaggcaga accacagact attcgaggta tgaatatacg
     8341 caatttggtg gcccttctaa aggaaaaaat gcaagtatca acttcaattt ttctaatact
     8401 ttggaaataa aaataaaatc taaaaaagat acaattactg gaacacggaa aataacctta
     8461 ttagatgctt tgaattttgg tgttggatat aattttgctg ctgaaaaatt caaattaaat
     8521 ttctcaggaa tttcaggaag tacacatatt acagacaaat taaatatgaa tttcagagtg
     8581 ggtatggatc cttacgcact agatacaaat ggtgtacgga taaatgaatt ttattttaaa
     8641 cataaaaaaa gattttttcg atttacagga atggacataa gcttgaatgg ttcttacaca
     8701 tctaaaaaat ataacaatag tggaacgatg cgtatgcaac aagaacaagg tttggattta
     8761 tcgccaactt tcagaaatat ttatcaatta ggttattata attttgatat tccttggagc
     8821 attaattata attattcttt gaattggagc aaatcatttg tcaataaaaa agatacgaat
     8881 atcattacgc aagcaatagg aattggattg gattttaata tcacatctaa gtggaaaatc
     8941 aatgtaaata caggatttga cctaacaaat aaacgagtaa ctagaacaga tattagtgtc
     9001 gttcgtgatt tgcattgttg gcaattggaa atgcgttggt cgccaattgc tgcacaacaa
     9061 tctttcttta tcacaattta tgtcaaatct cagcaattta atttcttacg attacaaaaa
     9121 caaaaatcct tctttgactc aggattcttc ggttcaggtg gtgctggtat gggtggttta
     9181 ggaaatatga tgggtggagt tgccaataca ttctaaaaat tttgtatttt tacataaatg
     9241 gattaaaact atgctcgaat taattaaaga tttaagtcag tttctatggg aacgcaaaca
     9301 atggtggtta tttccattga taattagttt aattttagtg gctttgttaa ttgtatttgg
     9361 tggttcaact gctgtaacgc catttgttta ttccatcttc taatgcaatt tattaagcaa
     9421 aataaagagg tttttattca aattattgtc ttcagtatta taggcattgg attgcataaa
     9481 acatttttac tttattggtc tttagggtta attattttat tatttatacc gcaattggct
     9541 tccaaatata ttgacatttt aaatagattc attcatttat ttggctatat tttaaaaaca
     9601 atattattta gtgttttatt tgtattcatc ctttgtcctg tagccatttt caagaaaaaa
     9661 agtaataaaa tgggctatat tcatcgaaat ataattttga gcaaaaaatc atttgaaaaa
     9721 atgtggtaat tgctaatatt cagataccaa agaatttcta aaaaatcact atctttgcaa
     9781 caattttaaa gcaaagtttt atgtttgaga atttacagga taaattagaa ggagcgttta
     9841 aaaaccttaa aggtcaagga aaaataacgg atattaatat tgctacaacc gtgaaggaaa
     9901 tccgtagagc attggttgat gccgacgtaa actataaaat tgctaaagaa tttacagata
     9961 caattaagga taaagcatta ggacaaaatg tactaacagc agtttctcca ggtcaattaa
    10021 tggtcaaaat tgtaagcgat gaattgaccg aattgatggg tggaaccgtt actgaattga
    10081 acgtaaaaga aaatccaaca attattttaa tttcaggact acaaggttca ggaaaaacta
    10141 cattttctgg taaattatct tactatttaa aaaataaatt aaaaaaatct cctttattgg
    10201 tagcttgcga cgtttataga ccagctgcga ttgagcagtt gaaagtattg ggtgagcaaa
    10261 ataaagtgga agtttatgct gaaattgaga ataaaaatcc agtagaaatt gcacaaaatg
    10321 caattaatta tgcgaaacaa aagggttata atgtcatcat tatagatact gcaggtcgtt
    10381 tagctgttga tgagcaaatg atgcaagaaa tttatgatgt aaaaactgca attaaaccaa
    10441 cagaaacttt atttgtcgtg gattccatga caggacaaga tgcagtgaat acagcaaaag
    10501 ccttcaatga tagaattaat tttgatggtg ttgtattaac caaattagat ggtgatacaa
    10561 gaggtggagc tgctttatct attaaatata ccgtaaataa acctataaaa tttgtaagcc
    10621 gtggtgaaaa aatggaaacg ttagacgttt tcttcccaga tagaatggca cagagaatat
    10681 taggtatggg tgatatcgtt tcattcgttg aaaaagccca agaacaattc gatgaaaaag
    10741 aagctcaaaa attaagtaag aaaattgcta aaaaccaatt tgattttgat gactttttac
    10801 aacaaatcaa tcaaatcaga aagatgggta acttaaaaga tttattaggt atgatacctg
    10861 gcgttggtaa agcaatgaaa gatattgata ttgatgataa tgctttcaag aaaattgaag
    10921 ctatgatatt ttctatgacg cctcatgaaa gagccaatcc agatgtttta aatggcaaaa
    10981 gaagagaacg tattgcaaaa ggaagtggta gttcaattca agatgtcaat aattttgtca
    11041 agcaatttga ccaaatgaaa gtgatgatga agcgtttcca aggaatggga atgaaacaaa
    11101 aataggctta atatactttc aatgattaga tactttttta tctaagtcgt aagtgtaaac
    11161 attgtaagga aaaatcaatt ttgcaaagaa attagcaatc aaacagcaaa ttagtaatgg
    11221 aaaaaataat tcatatccat taggcactaa attgcatatt aaaaataaag aagtaaatgg
    11281 tgcataaata gcagctgata aagttgttgc agctccaatt aatgcaaagt ttaataattg
    11341 taaggaagtt cctaaatata cattacaaat tagagcaaaa gcaacaccca aaaatgcacc
    11401 agcaacaata ctaggtgcaa agacaccgcc atcgcctcca gctcccaatg ttaatgatga
    11461 tgctaatggt tttagaaatg caataaacaa taaaaacaaa attgagtaat tatgataacc
    11521 accaatcatt tcttctaatg catgataact atcaccataa ataaatggaa aaaaacaaat
    11581 catagtgcca acggctaatg ctccaagatt gacacgaata aaattattat ttattccaga
    11641 gaagtagcct ttaattctaa gtaccaaaat agtaaaatag atagacaaag agccaccaaa
    11701 caaacttaaa actatcataa aaggaatagc ataccaattc caaccagtaa ctgcaatggg
    11761 aagaataggt tcaggatgta aaatctgtaa aaaaatccac gcaacaaagg ttgctattgt
    11821 gcaacatagt acaaaagctc gattcatttt ttttgcaatc acttcccaag caaaaagcca
    11881 accagctatc ggactattaa ataaaattgc aataccagcg gcaacacctg cactcaccaa
    11941 ttctctttta taagctcttg ctccaaaatc ttttttataa actgtattgc ctaaagttgc
    12001 agttgccacc accgttgaga cttcaattcc tgttgaacct ccaaaaatta cagtcaaaaa
    12061 accattgata taatgtgagg gtaacttgaa taatggaaga tggtctttag gataatcgag
    12121 tgtattataa atctcactaa tgcctttgtt tttcttatta tgaaaaacat attttcttag
    12181 ataatatata gaagtaatac caatagtcgg taggaaaata taaactaatg gatgatattg
    12241 tttcgcagag tgaaaaattt gatgttcaaa atattccgta attcccatta agctaattgc
    12301 taatagtgaa gcaaatagac ctatggtaac agcataaatt attaaaacaa aactatcctt
    12361 tttattcatt ctactcaata ctgaatcaat actcataagt tctttcatta agtattgatt
    12421 ggtttttatt aggtataaaa ataaaatgaa tacctaaaat tatacaacag acttataaga
    12481 aattataaaa agatatttat aaattcattt tatattaacg tgattcaaat cgtattaaaa
    12541 aacaatcata atttaattgc tctggtcatt tctctttttc caattggacc tggcaaactt
    12601 tcaatattaa aaccaactga ttttagagtt ctttttacaa ttcctttagc acaatatgta
    12661 accaaacaac cgccatcatc aagcatatca tacatttttt ggaacatttc aactgtccaa
    12721 agttcatctt gtgccgaagg tgcaaacgca tcataatata tcaaatcaaa taactgagaa
    12781 ggcttataat cattaatatc ttggagatat tttgtaaatc taaaattctc tccaatattc
    12841 aacattttat tagacttgat ttgatgaagt tctaaaaaat tgggatgatt aaagtttgga
    12901 aattgtacta atttatttat tgattccaat gtcaatgggt ataactcaat tgcatgataa
    12961 tctacaaaaa cattatgctg ttgtgcataa ttcaaagtta gcaaggcatt aagcccagtt
    13021 cctaaaccca tttcaaaaat ccggatagat ggttgttgct tggctttata atccaatcct
    13081 gcttgaataa atacatgtaa agattcttgc attgaaccat gaattgaatg ataatgttca
    13141 tcaaaacgct caacatacaa agtgggggaa ccatcatttg ttattaccaa atcaatatcc
    13201 aaaatcctta atttttatac aatttagaat agtaaatttt gttcttattt tgcaatccta
    13261 agatgtaaat attattagat aataattgta taggaatatc aatgctattt ttattgctat
    13321 ctatttgaat gtttttctga aaaacaacac gtccatctaa ttgcactaaa accaaattat
    13381 aattacctaa aattgaatta ttatgttcaa tatgtaattg attttgacta aaataagatt
    13441 taatttgtaa aacatttttc tttatgcctg tcatattctc aaattgaatg ctatctacaa
    13501 gcgattgtat gctaacacca gaatttaaga ccgcttctat tcgataatac aaaaccgtac
    13561 caatggcagc attgctatta tgtataaaat aataatcaga agtatctgaa atattatgca
    13621 agctataaat actggcaatt tcttgaaaat gctgaccatc tattgaatat tgaatattat
    13681 attttaaaac attagtatca ccagttgtat tccaagctaa aatagcatca tcgcccattt
    13741 gatctgcatc taacgttaca atctgagtgg ttggaacaaa tgttgggttg atagcttttg
    13801 aagtcaaata aaactcacca aatttttgcg tattcatttc aacataatag ccattttcaa
    13861 atggcacaaa atataaacta tctgtttgga ttgagacaaa atctccttta atataattat
    13921 tttcaaatac tacatcttga ttaaagccat tgtatcttaa tgcagcaata tcccgcatag
    13981 aaaataattg actatctgta ttcaataaca ttaggtattc ctcatttgtc aaatacaatc
    14041 taattttaaa atttccatca acttgatttt ctgtattaat actccaattt cttggcaata
    14101 atgctttacc ttgatatttc ctgacttgat tacttgcaaa atagcctact gttatatttc
    14161 ctaagacttt attatcatca taaatttcgg caataatttt atcatctttc aaaattggta
    14221 tccaaccatt tccattagaa attgtatcaa taaatacaga atcatttaaa ggaatagtat
    14281 aatttgttac ataaacatgc caatcatcaa tagccaaacc ttcgccaact ataccttcat
    14341 ccgattgcaa cctaactcta ataaatacat gttgattatt tagtgttgta tcaatattta
    14401 tttttgatga agcaacttgc caaggcatca atgttttatc ccattttgct gcaccattca
    14461 tatttccata aaaattagta ccacaattag cacaacctat tctattccat tcattgccat
    14521 caatagaata ttctgcccaa gctgagtcat ataagttttc aatatctaat aaatgattaa
    14581 aggcaacaga tggtgtattt ttaaggcttt gcatattata acaacctaaa tacaaactcg
    14641 aattttcact ataattataa gtatcaaaag tagaattcgt ccaagcttta ttgccttgag
    14701 ctgccatttt tgtataagtt ttatttggag tactccaagc ccaacttgag ttttgtcctt
    14761 ctggaataca taaacttgtt tgttcaaaat tattcaagta aggcaacgaa tctatatata
    14821 aattagcaat aaagcttatt gcatctgaag tatcattacc tttatatttg tcatcaacat
    14881 catcagcaat aacgtgaact acatatttac cactttgagt tatagctaag gtttgattaa
    14941 aagtataatc aaaagtttga taaggcaata taatatcact aatggtttca ctcactacat
    15001 ttgcattatc gacaatataa gtaactttta agttgcttcg tgcttggtta ctttggttga
    15061 gaattttaat ttttattgga atatttttgg actcatttaa gcataaaata gagtcttttc
    15121 cttctgtgct atttgctaag tcattatttg caacatacaa tttaatatca tcaaaagtat
    15181 aacctccact tttcgttaat tcattactag ctttagtgtt tgttgtagct aatttaattt
    15241 gaaatgaaga agatactgtt tgtccattat ctacatataa taaattgaat agatttattg
    15301 cttgcactct attatattct cctttaggtg ctttattttg aaataaattt aaaattggta
    15361 tccaagtagc cgtatcattt cctctagcat aaacaatatt atcagtcgca gtctcgccgt
    15421 gttgcataaa acttaaatcc aagtaaataa tcgaatctat ataatttgct aaattaagcg
    15481 taataatatg actactttta atacttccag tattgctata attatctaag gtcaatgctc
    15541 gttttccaga caatgcgtac atattatctc taattctcca acgaccatat ttgtcggtat
    15601 taaaatcggc gtattccata ccatcaatcc caaaaatatt agtactataa gttgctccag
    15661 aattttcaaa agattctgtc catggtaacg aaattggttc gttggctaag tgctttaaag
    15721 tataaactaa ggtatcatta ttaggcaatg tatctaaaga gaatgaagaa tatgctttta
    15781 agttataatt tccagctaaa ctcatatcat aatcattact tgcttgtact tgaatcgtat
    15841 catttgagtt tatagttctt gaaattgttg aagtatatat tgttccttca ttgacttgat
    15901 aagataaatt aaaatttgaa atcgggtttg aaccataatt cttaattaca aaaacaggtt
    15961 ttacttgatt gcctaactga gaagaggtat gcatacgacc gcccattgga tttattactg
    16021 ttaatacacc aatgtcgttc aattgagaac atgctgtaaa agatggcgta aaaatcttgg
    16081 cagtactttt attactttct acaccatcaa ctgttgcagt catttcaaca acataagatt
    16141 tgccatttac taatcctgta agttgataac ttaatttaga agtggtatct acttttcgcc
    16201 aaacgaaatc atcaaaaaca taaatggtat aatattgtac atttggaatc gctggccatt
    16261 ttatatttgc tgatttatca caaggtgcaa ttgtaataaa gtttgcatta ggtctgaaat
    16321 tgatattaaa tgtagcattt gatgaatctg aaaactgtga atttattgct ttaatcaaag
    16381 catttttggt atttatattg ggtgttgtcc acgtaaaaaa tgcatcacgg ctatgtacat
    16441 tttttataaa tgtccaattg ataccactat caatcgaata ataaaaacta agagaatctt
    16501 ctacatttct tgtattccat gtaacgttga agtttgctcc tgcatctaat gtttctccac
    16561 catcaggata gacaatatca ataccattat tttcaaacgt ataacttatg ctatattgct
    16621 gttggtcagt tacaatatct gttccattta ctaaaatggt atatgttcca gcaagtgcag
    16681 aatccaaagt tacatattct atattattca aattatcgat gcctctttgt gctggatttt
    16741 gataaaatat aggatttaaa acccagggta gaatggtatc attattgggc gttattaatt
    16801 ttaaatctac attatttact aaaattttat gttgtaaagg ataagcagca gggtctgtcc
    16861 aacataaagt aattgaaaca aaatcagcat tttgagttaa tgtaaattga tgattatttt
    16921 gattttgatt actaatgttt ccaataataa atttattatc aataatattt tgaactgctt
    16981 ttttagcatc agggcgacca aaacctgctc cataatctaa tccttttcga tacatatctt
    17041 cagccgtatt acataacaca gctttgacta agcttgaagg tggcagaacc ccattttgta
    17101 atttatactt ttgttgaact aaagttgcaa taccagctat ttgtggagct gaaaatgaag
    17161 aaccatatac tgttgaaaac gcagttcctg gagcacttgc agtatatgca aaaccttttg
    17221 ccactaattc tggttttaat ctaccatctg ttgttggacc acgcccactt gtttctacca
    17281 acttttcatt ccgatataaa tatcctacag ttattgcatt tttagtacct tggtatccaa
    17341 tatctaacga ataaaaagta tcattaattg cacaaagatg agctgtattt cctgaggcta
    17401 caacgtgtaa aacattggga tattcatacg ctaacttatc cattttacta gcatctaaag
    17461 tatataaacc tgaagccgat ttacaatgtg attgttcaaa attataggaa tgattggtta
    17521 cattcaattg gtattttgaa attccatctt taatttcttg aataatattt ccccaattat
    17581 cccaagcata aatattggat ttagatgcag caccactatg caaataactt gtatttccac
    17641 acatagctgc tgcacctgca acttgtgttg gatgcacatt atagggcgaa aaactcaatg
    17701 ttttatcaat attaaagact ctggtatctt ctaaattgat atgagttcca attgcgccac
    17761 cgtcccaaat accaatattc atattttctc catttagatt cagtcctcct tttgaaattg
    17821 gcatattgac acttggcaaa cgatccataa tatatgaatc tgaaactaat aattgtttgt
    17881 ctttataatc tttgcttatc aataagacaa aaggtaactg tgcaagttct gctattttac
    17941 tggttgtaat ttgtaattta taaatatggt catcttctat aaaatcatat aaactgacat
    18001 tcaatgattt taatatttca atagcattta ctttcttaat tgtacttgca aattgtactt
    18061 gtatattttg tattgtagca gttttatcta tatcgtcaaa tactttactt tcaggttgta
    18121 cataataaat tttacttgca attgagttaa ctttttcttt tggaatatta attaggtatt
    18181 gatgatttcc aatataagtt tcaatatttt tggcgtatga ttttgctata ctttcagata
    18241 caactatgta tgctatatct tcggaaacaa aaaggtgatt gtctaatttt ttattatcgg
    18301 aaggttgaat ttcaaccaat tgaattgctg aaaaaagcaa tgtatgtaca taaaatgtac
    18361 acaaaagcaa cagtataaga ttccttttca taaatttatt tttagatatt actactaaaa
    18421 ataataaata ttttggataa gtcaaattaa tagaaagttt cttatacttt taattctaaa
    18481 atattatttg aagtacaagc tatattgcta aatttaagcg tatcaagtat tgctatactt
    18541 tccaacaatt catgatatag ttgttctaaa tcagtatcta ttttcatttc atttacaatc
    18601 atttcatttt ctaatgtatt tgtttcattg taaataaatt tcaataaaaa atcaggtgta
    18661 aaaatatctg acataaaatt tctttttaat caataacgaa aaaaaaagaa gaatattgta
    18721 ttctagaaaa ttaaacacgc taattaaaaa aatttcaatt attgtttgtc aaaaaatgca
    18781 ttaacatcat tcaatacttt ttcaggatac tcagccattg gcacgtgtcc acaagaatca
    18841 taaactatca tagtactgtt tttaatatct tgatgaaagc gttcagcaaa ttttgagttt
    18901 ataataatat cttcttttcc ccaaagtatt aatgttggaa cttgaatgtt ttttatttta
    18961 ttttggtcag gataattttt agaattggac atacaaataa tttgttccaa agcacctttt
    19021 ctattccaaa aatcattcat tcgctgcgtt ttttcagcac tcaaattgga tttatgataa
    19081 aatactctat tcaccccatt ttctgtcata aaataaggta ctcctctttt taagaatgca
    19141 gtaattattg ggttttgaaa tcttttaaca cctgcagatt tttcaacttc tttcatatcg
    19201 taaccagcac tattcaacag aaccaatttc tttactttat caggatgttc taatgtcagt
    19261 acccacgaca tcattccacc taaggaattt cccatcacat aaaaactatc taaatgtagc
    19321 gtatctataa gaaaattaaa atattcatta taaatagagt taaaatctgt ttgcggtgta
    19381 ggtttaccag gaaattctga caagccaaat cccggcacat caattcttat aactttataa
    19441 tttggattta ataagtgatc taatttttca aaatctttaa aacttcctcc aaatccatgt
    19501 atcatcaata ttgggaagcc ttttccactt tctgtataat gaatttccgc acctttccac
    19561 tgtataaatt tcgagtttgg ttctttatat ttttctttta tatattcacg tgataaggtc
    19621 aaatcttttc ttagaaaaaa cacataaata cctacaaata ataagagaat taaaaagaaa
    19681 aaaagtagtt ttttgaacat aactgaaatt tataatttat aaaaataaga aaatcaattg
    19741 gtatctatat tttaaaacaa gttaaatttg aatataaaaa tccaaatgat tatttttgga
    19801 ttcttaattt ttacagctaa tggcaatctc aatttttgat acgttaatta aaaaagcgga
    19861 agcattaatt gcttcagatg gaaaagtatc tcaattatta gatgagtttt ttttaatggt
    19921 aggaaaatca actgaaaaat tatataaaat tcaagattct gttgttacat taggcagatt
    19981 gttgagagct tgggtaaaag gtgattataa aaacatcagt acttcctcta ttattgctgc
    20041 cgttgccgca attatttact ttgtaaatcc attagattta atacctgatt tcattccaat
    20101 tattggacaa atagatgata ttttagtatt gggttatttt gtaaaaatat tgaataaaga
    20161 aattgagaaa tttatggctt gggaagctgc cc
//
LOCUS       DAOQCW010000037        19944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_980_length_19944_cov_5.708633, whole genome shotgun sequence.
ACCESSION   DAOQCW010000037 DAOQCW010000000
VERSION     DAOQCW010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 19944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19944
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_980_length_19944_cov_5.708633"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="PLS16_04865"
     CDS             complement(<1..840)
                     /locus_tag="PLS16_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNJ60442.1"
                     /translation="MIAVIPVAGVGTQLRPHTYTQPKSLIPVAGKPILSYIIDQLLEV
                     GITEFVFVIGYLGEKIKEYVDSEYPKINKSFVFQEQREGLGHAIWLTKEVIPNDSSII
                     IVLGDTILDANLKEFIKIKNSALGVKRVDNPSLFGVAQLNDKSEIIQVVEKPKIPKSN
                     MALVGLYKINEYGLLIEALEEIIKKKKKVNNEYLLTDGIMTIIKKGNKVDAFKVDNWY
                     DCGQKEVLLQTNALLLKNKKGNTKNNKLLKNTIIIEPVVIGKNAVVENSIIGPNITIG
                     ENSV"
     gene            complement(877..>1122)
                     /locus_tag="PLS16_04870"
     CDS             complement(877..>1122)
                     /locus_tag="PLS16_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60443.1"
                     /translation="IEFMNWDNANKLQNTIPKQLFVTLNDEESLIYEIIKTHKKIHID
                     DLLGKTPYNLSNLSNILLQLELQDVIKPIPGKFYTLQ"
     assembly_gap    1123..1222
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1223..1815)
                     /locus_tag="PLS16_04875"
     CDS             complement(<1223..1815)
                     /locus_tag="PLS16_04875"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNJ60444.1"
                     /translation="MKDDLLYALALYQTVGVGTITALNLLEYFGNYESIFSAGITKLE
                     KVKGIKKKVALEIFESRKIALKKAESELNFIENNNIQVLFYQDEEYPQRLRNCVDAPF
                     LLFAKGNYDFNKSKMIAVVGTRHATEYGKKMTQQFVEELSAYDISIVSGLALGIDSIA
                     HQSAVKYNLQNIAVLGHGLDMIYPASNKKLAEKIQENG"
     gene            complement(1939..3555)
                     /locus_tag="PLS16_04880"
     CDS             complement(1939..3555)
                     /locus_tag="PLS16_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60445.1"
                     /translation="MKKTLLYSLIIFAFTLSFAQQQNLPLNFEWNNRLQRFINEDNRI
                     HTAIKPYLQSEVNKSLVDSLNELGLPDTTQAGFKFGITSGDLISFRGKKKRNVYIAIN
                     PIVDFEVGYNTAKKSLQHSIGYGAQANFDLGKKLSLSFTYQGVTENYLNQISDYAKEY
                     GVLTGYRSAKFNGNSISSQMFSGYLSFSPNKYVNLQIGNDKNFWGDGYRTFLLGDNSS
                     NYPYIKFSTNFWRIKYVYLLNVMRYGQTSGLNVDNNTSNFKTKYGSFHYLSVDVAKWF
                     EFGFFEGVTWYHEDSSKVRGIEFSYLIPTLFIRPIEFALGSPDNVILGLNFKFKPSNR
                     QDIYLQLALDDMDVAAARRGRGFYRTKVAAQLGIKARDIFKVKHLDILSELNVVRPYV
                     FAHKVPAQNYTNYNQSLVHPLGANFWENVTMISYWKRHWQASAKFQFARKGIDLSPSE
                     HYGSNIFVSDYLISSDLGKAYNNKFLQGVKTNLINLEVRGGYLLNPKLNLALELVYNF
                     RNMKNDYIKQNYHYFGLGIRTNLFNRYSDF"
     gene            3644..4111
                     /locus_tag="PLS16_04885"
     CDS             3644..4111
                     /locus_tag="PLS16_04885"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HNJ60446.1"
                     /translation="MNLRKNIRLINYYPPYIGAGISVKSVNEDFTQIDVQMKMRFWNR
                     NLVGTHFGGSLYSMCDPFYMFMVMEHLGRDYIVWDKSACIDFKKPGLGTVTASFMVTL
                     DEIEKIRQYVEENGKGNFTFNTNVLSETGEIVAAVEKVVYVRKKQTKSIDTNK"
     gene            complement(4164..5363)
                     /locus_tag="PLS16_04890"
     CDS             complement(4164..5363)
                     /locus_tag="PLS16_04890"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNJ60447.1"
                     /translation="MNLEIPKKFKGIQGFALTAAQSLFRPISRKYDLAEHEYPKELDL
                     FAAGLNGMNEGSGGAVGGASSKKEEVQAGEVKNGGNMANCINIQTMCYGDVGLLLTIP
                     GQGLGNAAISAVANPEQLKRFSKSWAAMAITEPNCGSDSSAITTTAVKDGDHYILNGE
                     KIFVTSGERCDCVVVWATLDKAIGKAAIKSFVVEKGTPGMEIARLEKKLGIKASDTAA
                     IVFTDCRVPAENLLGSPEIDVKKGFGGVMETFDNTRPFVASMANGVAEATIERTKELL
                     EDSINLDYATPINNVKAVEAEIYRMEAEHEAARLLTLKAAWLADNRIPNSLEASVCKA
                     KAGRITNNLTLKAVELCSSLGYSYQELLEKWARDSKILDIFEGTQQIQQLIIARRILG
                     KSSAELK"
     gene            complement(5412..6734)
                     /locus_tag="PLS16_04895"
     CDS             complement(5412..6734)
                     /locus_tag="PLS16_04895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNJ60448.1"
                     /translation="MSKKKEAKKTDFSSYFEFYNNLLDSKIGKTLQLRKLSEKIVYEG
                     AKNGVKAGAAATKVFQTFGSGKAKRLEPKKAKDLFDLNYNEEQLMIQESIKQLADKIR
                     IHAEKDDYNGKISEEILREYNELQLAFMQIPEELGGIMKEKSTTTQMMMVETLAYGDL
                     SQAFALFSANSVINAIVQWGTDSQQQILLKDFVEDNAPKASIAINEPTALFSPQTLKT
                     KANKDGNQYIITGEKNMVTFAESAQYFLVAAQTEGGKNQLFFIDRTADGVSTSADVSM
                     GLKTAELGKIVLDNVRIDESAMLGGSKGVNYQEFINYSKLGWCSLAVGCSQAVLDYVI
                     TYANDRYAFGEPISNRQAVAFMIADIKIELDSMRILTQRAVARAEQGLEFQKEAYLAH
                     VLCSDKSMQIGTNGVQLLGGHGFIKDFPVERWYRDLRAVAICYNGLHL"
     gene            complement(6828..7580)
                     /locus_tag="PLS16_04900"
     CDS             complement(6828..7580)
                     /locus_tag="PLS16_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60449.1"
                     /translation="MFLLITHQLIFAQQKEFILNAKIVDENNIPLAHVDIANRRLQLS
                     FAADKYGYFTTNTQMSDTLLLFKSGFLPQKLIVNDTMFDQNKIILVRMFHVPIELTEV
                     QITAIKTHQQIRKSINNLYVPNTDLYPNAILFQNPISYLYELISRKEKEKRLAANLQT
                     EFSKRSVLKELFRLYNAYDIINLPDDDYDNFITYLNMPYEFMQSVSDYDLAVTIKYKF
                     KEYRNDKNNWIKQEIYPAALDDLDRIRQQNKN"
     gene            7674..8234
                     /locus_tag="PLS16_04905"
     CDS             7674..8234
                     /locus_tag="PLS16_04905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNJ60450.1"
                     /translation="MEINNLDDESLIDLYQKHKDNDAIGILYKRYSMLVFGLAFKYFK
                     QEDVAKDMVTEIFMKVMDKLSQAKTQKPESFAKWLYIVSRNEIISVLRKKKIQVDTVD
                     EINSEKFMESEEEIRPIIKDIDEKQLDAAMSNLNEEQNKCIHLFYLQEKSYQEVASLT
                     GYDIKKVKSYIQNGKRNLKMFLTKKN"
     gene            8240..9004
                     /locus_tag="PLS16_04910"
     CDS             8240..9004
                     /locus_tag="PLS16_04910"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ60451.1"
                     /translation="MKKEELKKYLEQTLSDKERNALEQEMQDNPFLNEATEGLEAWTQ
                     QGNADFDALENELNKNIDDKIDSKNLEIKKVVRMPIFRIIAVAAGIIGILFLTVTYLH
                     QQRMETEALFEANFKVLTQIDGAVRGEDAEIESNMNEEKKAVYYYERENYKEAIVHYE
                     KALQSQPDNNQMQLYLAVAYLANYQATDAIQILSENDFSNSQYKQDRNWYLALAYLKN
                     KDLKNAKPLFEQLSAFDNYYQNSSKKLLKSLEGKVL"
     gene            complement(9008..10648)
                     /locus_tag="PLS16_04915"
     CDS             complement(9008..10648)
                     /locus_tag="PLS16_04915"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNJ60452.1"
                     /translation="MKYSIAFLFVLISITTNAQTTLVRGPYLQSLTDKSIKIMWRTSD
                     STQAVVKIGYAPNALNRTIIDTHNIKDHIVLVDGLSAREKYYYSVGTDDVNVLAGGDE
                     QHHFFAAPSVGDTTGFRFWVIGDFGKANSRQVRVRNWFQTYVKDNKVDAWLWLGDNAY
                     QDGKDVEYQEKVFGRTYGYDSILRFLPSYPIPGNHDYNSVNQSKPAEKHTGPYYNNVE
                     VFRNAEMGGIPSKTEAYYSYDFGNVHFMALNSELYTYSIFWDLGSPYKTWIENDIKST
                     DKKFIVAYIHQPPYSKGSHDTDDFYQIFMQNTREKIVPILEKYGVDVVLSGHSHVYER
                     SYLINGHYARSSYFNKEKVVVDSTDGNPDNGNVYTKYTYGPLKGKGTVYSVIGNSGNY
                     EEHNGKMHPVFTTKYDDTGDNGAGSMILEVKGNELTGTYYTLDGVLFDKFRIVKQDSS
                     TISGIKQNKNIKSLKVYPNPFSEQVTLEVSLDKAAVLDVYIQNLTGELVADKIAFGRA
                     NKGVNLIEINNIGDLPAGNYIITIAQDGIFTSKQVVKL"
     gene            complement(10869..11729)
                     /gene="lipA"
                     /locus_tag="PLS16_04920"
     CDS             complement(10869..11729)
                     /gene="lipA"
                     /locus_tag="PLS16_04920"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNJ60453.1"
                     /translation="MNEVTEHKRTPKPSWLKVKIPMGENYKKVKEIVDSHKLHTICQS
                     GNCPNIGECWGRGTATFMILGNVCTRSCSFCAVPTGRPFFVDEDEPRRVADAIRLMGV
                     RHAVITSVNRDELPDKGAKIWNETIKQIKLVSPNTTIETLIPDVKADWNALEVIISAG
                     QEVVSHNMETVKSLYRKIRPQAKYERSLEQILRTKEYGKRTKTGIMLGLGETKEEVYE
                     AMDDLVAHGCDVLTLGQYLQPTKMHTPVIEFITPELFAHYKEVGLGKGIEYVESGALV
                     RSSYHAERHI"
     gene            complement(11731..12522)
                     /locus_tag="PLS16_04925"
     CDS             complement(11731..12522)
                     /locus_tag="PLS16_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60454.1"
                     /translation="MQTSLAQYNFVEQQESKKTSFSNRTWLVGAKIRSDGLGVHIDIT
                     SGRKYRSAILYQFELGYYKHNAQIKQHSPYAGSSDMQFKPYFFGKQNNLFAAHFNIGQ
                     KILIAEKAKKNGLRLFYHYSGGFSLGILKPYMLTVIDTNSLSYNPVEQTYTFSETINV
                     AYNSNETYYLNYIHYNQFGQTFEAIVGGAGFRKGWNLKFRPGLHIKTGLSFDWSKNEG
                     LIKALEVGFSCDVYFNKVPIMLVNNKSVFPSVYLGFQIGRKFKGK"
     gene            complement(12634..12951)
                     /gene="ytxJ"
                     /locus_tag="PLS16_04930"
     CDS             complement(12634..12951)
                     /gene="ytxJ"
                     /locus_tag="PLS16_04930"
                     /inference="COORDINATES: protein motif:HMM:TIGR04019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HNJ60455.1"
                     /translation="MDWINLNSIEQFDELLENQKVFAVFKHSTRCPVSSTAKNRVEKE
                     WTHSFPIYYLDLLNHRAISNYIAEKTGVQHQSPQLIVLENKKVIYDASHSFIFVNDIQ
                     TSS"
     gene            13171..14532
                     /locus_tag="PLS16_04935"
     CDS             13171..14532
                     /locus_tag="PLS16_04935"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNJ60456.1"
                     /translation="MANYHFIAIGGAVMHQLALHLKAEGNEVSGSDDIIFEPAMTNLK
                     HAGILPTSFGWYPEKINHNIDAVILGMHAKADNPELIKAEELGLKIYSFPEFFYQQCK
                     EKTRIAIAGSHGKTSITSMIMHIFKNYDKRFDYLVGAKLDGFKYMVSTSKDNDYAIIE
                     ADEYLTSPLDLRSKFLHYFPQIAIISGIAWDHINVFPTFDNYLETFRKFIASIQPDGY
                     LIYNQEDEELRNLVQSVDTKHIQLIPYSPFDAVIENEAVKIKYEGQSFPIEVFGKHNL
                     SNLKAAFEVGKLANIDVKDILNSFQTFKGAAKRLECIYQDNINKITIYRDFAHAPSKV
                     NATVQAVREKYSNRKMIAFFELHTYSSLQENFICQYAHTLDVADVQFIYIDAEALKIK
                     NKENFDPNWLKEQFANDKIIVLKDKADIIQHLKQAITQNCVVLLMSSGNIANIQIEEI
                     LHS"
     gene            complement(14538..15299)
                     /locus_tag="PLS16_04940"
     CDS             complement(14538..15299)
                     /locus_tag="PLS16_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNJ60457.1"
                     /translation="MANIILITGATAGFGKATAIKFAAAGWNIIITGRRKERLESLEK
                     NLKSTYNIDVLSLCFDVRNLEEVKSAINSLEEKWQAIDVLINNAGLAAGKGPIQEGLY
                     DDWEQMIDTNVKGLLYMTREIAPLMIKRNQGHIINVASLAGWEAYGGGNVYCGTKHAV
                     RAISRGARIDLVQHNIKVSVVSPGAADTEFSLVRFKWDKDKADSVYEGFQPLQAEDIA
                     NSIYFMATQPAHVNIEEIFILPTAQATSNVIHKTK"
     gene            complement(15343..16998)
                     /locus_tag="PLS16_04945"
     CDS             complement(15343..16998)
                     /locus_tag="PLS16_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60458.1"
                     /translation="MPFELQTFFYKDKLKPFSYTNLFLGIILILIAGLRLIRNFNNLV
                     DISFDDEVQYMRYGMELFHNIRSDWGPSYNMWYKLLSLFEQRPIELYLLNYKVVIILL
                     PICLFAFLYVYGISFFISIWIGFSILISTTNILTYPRISHVVVSLFLVVLIINKLWIK
                     SKSRQLILLCFTIFIAAFARPELMLAFLILSAFTLFYIIKNESLKKHIVFALPFVAIA
                     ILIFAVFRLPSDSFKGIDRAYIAFCQHYTIKNIIFNKGHFNQFIDWIAISKTQFPGCN
                     TFTDIVINFPLVVIKGMFVNIGFYLQLIISISTDILFPFQLLPFNKIVLLGYGLVVLF
                     ILYTLISKKQRLNVFNAIQSNKYLFLVLLIFIFPSIISSLVFFPRMHYGIFLLPILSF
                     VFAILIDNLAKGYKIRIGIVLFVFTLFMYKMPSIKKYNTPKLLTNDECPTQSYKEVIK
                     DLNQHTDKPHVIFSNVLSFSLMIDKNFTDFSAEYDYDNNKTFIEQMKTHQVDYILQTK
                     FLTEDRRLSKDSTWLQFMANPQAYGFKKKKLFDDCETYLLYKE"
     gene            17068..17394
                     /locus_tag="PLS16_04950"
     CDS             17068..17394
                     /locus_tag="PLS16_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HNJ60459.1"
                     /translation="MTIQEAQNQIDQWVKTYGVRYFSELTNMAILTEEVGEVARIIAR
                     QYGEQSFKASEKDKNLADELADVLFVIMCLANQTGINLTEALQKNLAKKTQRDAERHF
                     NNEKLK"
     gene            complement(17387..18607)
                     /locus_tag="PLS16_04955"
     CDS             complement(17387..18607)
                     /locus_tag="PLS16_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60460.1"
                     /translation="MIDIRSRQKWFKIGVFNLMLVAAFGALMRYKTAHSFPFFNQSFL
                     LHAHSHFAFAGWVSHILYSGLTILIATYIDEQKLKKYKILIVVNLISAYGMLVAFSAQ
                     GYKAISITFSTLSIFVSYFYALFFILDSKQIPKGTTFKPWAIVGLLINALSSIGPFFL
                     AYLMATKNMNRDLTNSSIYFYLHHQYDGWFFFGSMALLVAMLPKSVPSLNKYLSVFAI
                     TIVPTYVLSILWIDLPKLLLVVATIAAFAQFIAWVLMLSKIWKYLKDFKNKYASFYIF
                     FYFAAAALTFKFLLQVLSSNQTVGDMVFSCRPIIIAYLHLILLGAFSMYLLAYGLYNN
                     YLKANNLAKLAFVYFFFGFVLNELFLVAQSTSWVTLIPVPYINHGLLGAALTLLFGAG
                     LFFISQIISEKKTI"
     gene            complement(18718..19419)
                     /locus_tag="PLS16_04960"
     CDS             complement(18718..19419)
                     /locus_tag="PLS16_04960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ60461.1"
                     /translation="MNIVISGVSRGIGKAIAIKFLKENHTVIGCGKSLEHLEELSNEL
                     KKYELNDKFFYKKCDVSNKEELQEFVDFVFQQVKKVDVLVNNAGVFLGGEMANEEDIF
                     LETMLQTNLLSAYWLTKSILPNMLQHQSGQIFNMCSVAGTKGYPSGGSYAITKHALLG
                     FSRSLREELKDKHIKVTAIHPGATLTDSWNGTDLPEQRFIPVDDIADLVFSISKLSKY
                     SVVEELVIRPQLGDI"
     gene            19485..>19944
                     /locus_tag="PLS16_04965"
     CDS             19485..>19944
                     /locus_tag="PLS16_04965"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ60462.1"
                     /translation="MAEYVYDSGGPLFHVGRYVLMVKRSIAKPEKAIMYWKETLRQMY
                     NIGVGSVVIILLISFFIGAVSSFQFAYNLSKSMVPMYYVGYVVKESMILELAPTVSCL
                     ILAGKVGSNLASELGTMRLTEQIDAYEIMGVNTFSYLIGPKILAAVTMVPF"
BASE COUNT         6822 a         3343 c         2770 g         6909 t
ORIGIN      
        1 aaccgaattt tcgccaattg taatatttgg accaataatt gaattttcaa ctacggcatt
       61 ttttccaatc actacaggct caataataat cgtatttttc aataatttat tattcttagt
      121 attgcccttt ttatttttta ataagagtgc attcgtttgc aacaacactt ctttttgtcc
      181 gcaatcatac caattatcaa ctttaaaagc atcaactttg ttgcctttct ttattattgt
      241 cataatacca tcagttaata aatactcgtt attgactttc tttttcttct tgataatttc
      301 ttctaaggct tcaatcaata aaccgtattc attaatttta tacaaaccta ctaatgccat
      361 attacttttt ggaatcttag gcttttcaac gacttgtata atctctgatt tgtcattaag
      421 ttgggctaca ccaaacaaac tcggattgtc cactcttttc acacccaatg ctgaattttt
      481 aatttttata aattctttta gattggcatc taatattgta tcacctaaaa caataataat
      541 actactatca tttggaataa cttcttttgt gagccaaatg gcatgtccta aaccttctct
      601 ctgctcctgg aaaacaaagc ttttattgat ttttgggtat tctgaatcca catattcttt
      661 aattttctct cctaaatagc caattacaaa aacaaattct gtaataccaa cttccaataa
      721 ttggtctata atataactca aaattggttt tccagcaact ggaataagcg attttggttg
      781 tgtataggta tgcggacgaa gttgagttcc cactccagca actggaatta ctgctatcat
      841 aattctacaa agttatacaa taaatatagg aaaaaattat tgtaatgtat aaaatttccc
      901 aggaattggt ttgatcacat cttgcaactc caattgtaat aaaatattag acaaattact
      961 aagattatag ggtgttttcc ctaacaaatc atcgatatga atttttttat gtgttttgat
     1021 aatttcatat attaaagact cctcatcgtt caaggtaaca aacaactgtt ttggaatagt
     1081 attttgaagc ttatttgcgt tatcccaatt cataaattca atnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnccattttc ctgaattttt tctgctaatt ttttattaga
     1261 tgctggataa atcatatcca aaccatgacc caaaactgca atattttgca gattgtattt
     1321 tactgcagat tgatgcgcaa tgctatcaat tcctaatgct aaaccgctaa caatggaaat
     1381 gtcataagca cttagctctt ctacaaattg ttgcgtcatt ttttttccat attccgttgc
     1441 atgtcgagtt ccaaccactg caatcatctt tgatttatta aaatcataat taccttttgc
     1501 aaatagtaaa aaaggagcat ctacacaatt cctcaatctt tgtggatatt cttcatcttg
     1561 ataaaataaa acttgaatat tattattttc aataaaattt agttcggatt ctgctttttt
     1621 caatgcaatt tttctacttt caaaaatttc taaagcaact ttctttttaa taccttttac
     1681 tttttctagc ttagtaatac cagctgaaaa aatggattca taattcccaa aatattctaa
     1741 taaattaagt gctgtaatag tgcctacgcc gacagtttgg tacaaagcta atgcataaag
     1801 taaatcatct ttcatctaat ttataagacg aaaatttata tgatttccat aagttaaact
     1861 aaagtaaaaa ataaatattt aacgtataaa accatttagt tttgtagtaa tgcatcaata
     1921 tcagaagaaa aaatcaattt aaaaatcgct gtagcgattg aataaattgg tacgaattcc
     1981 taaaccaaaa tagtggtaat tttgtttgat gtaatcattt ttcatatttc tgaaattata
     2041 caccaattcc aaagctaaat ttagttttgg attgagtaaa taaccacctc gaacttctaa
     2101 attaataaga ttcgttttta caccttgtaa aaatttattg ttataggctt tgcctaaatc
     2161 actcgaaatc aaataatctg aaacaaaaat attacttcca taatgctcag aaggcgataa
     2221 atctatgcct tttcgtgcaa attgaaattt agcacttgct tgccaatgtc tcttccaata
     2281 ggatatcatt gttacatttt cccaaaaatt tgctcctaga ggatgtacca atgattggtt
     2341 gtaatttgta taattttgag ctggtacttt atgtgcaaat acatacggtc gaactacatt
     2401 cagttctgat aaaatatcta aatgtttaac cttaaaaata tctctggctt tgatacctaa
     2461 ttgagcagca acttttgttc tataaaatcc acgtcctctt cttgctgccg caacatccat
     2521 atcatctaat gccaattgca aataaatatc ttgacgatta gatggtttaa atttaaaatt
     2581 taatcccaat attacgttgt ccggtgagcc tagtgcaaat tcaataggtc taataaaaag
     2641 tgttggaatt aagtaactaa attctatccc acgaacttta ctagagtctt catgatacca
     2701 agttacacct tcaaaaaaac caaactcaaa ccattttgcc acatctacac ttaaataatg
     2761 gaaagaacca tatttagttt taaaattaga ggtattatta tcgacattta atcctgatgt
     2821 ttgaccatat cgcatcacat tcaacaaata aacatactta attctccaaa aatttgtaga
     2881 aaatttgatg tatggataat tagaggaatt atcgcctaat aagaaagttc ggtagccatc
     2941 tccccagaaa ttcttatcgt ttccaatttg taagttcaca tatttattgg gcgaaaaaga
     3001 taaatagcct gagaacattt gtgaacttat agaatttcca ttaaactttg cacttctata
     3061 acctgtcaaa acgccatatt ctttagcgta atcagaaatt tgattcaaat aattttcagt
     3121 tacaccttga taagtaaatg acaatgagag cttctttcct aaatcaaaat tggcttgagc
     3181 tccataacca atagagtgtt gtaaactttt tttggcggta ttgtaaccta cttcaaaatc
     3241 tacaattgga ttaatagcaa tataaacatt tctttttttc tttcctctaa aactaattaa
     3301 atcacctgat gttataccaa acttaaatcc cgcttgtgtc gtatctggca aacctaattc
     3361 gtttaaacta tctactagac ttttattgac ttcactttgt aaatagggtt ttatggctgt
     3421 atgaattctg ttgtcttcgt tgataaaacg ttgcaaacga ttattccatt caaaatttag
     3481 aggaagattt tgttgttggg caaatgataa agtaaaggca aatatgatta gactatataa
     3541 gagtgttttt ttcataaagt attgaggtag ctaagataat attaatattt tgatattaag
     3601 tagggtttat aattactttt atctttgact tttgattttt aagatgaatt taagaaaaaa
     3661 cattcgttta attaattact atccgccata tattggagca ggaatttctg taaaatctgt
     3721 caatgaagac ttcactcaaa ttgatgttca aatgaaaatg cgattttgga acagaaattt
     3781 ggtaggcaca cattttggtg gctcattgta ttctatgtgc gatccgtttt atatgtttat
     3841 ggtaatggaa catttaggaa gagattatat cgtttgggat aaatcggcct gtatagattt
     3901 taaaaaacca ggattaggga cagttacggc aagttttatg gtaacacttg atgaaatcga
     3961 aaaaattcgt caatatgtag aagaaaatgg aaaaggcaat tttacattca atacaaatgt
     4021 acttagcgaa actggagaaa ttgttgcggc tgtcgaaaaa gtagtctatg tccgtaaaaa
     4081 gcaaacaaaa tcaatagata ccaataagta aataaaaaat gctccataaa tttatggagc
     4141 atttttcagt attcaattta agtttatttc aattctgcag atgattttcc taaaattctt
     4201 cttgcaataa ttaattgttg gatttgctgt gttccttcaa aaatatctaa aattttagaa
     4261 tctctagccc atttttctaa taattcttga taagaatagc ctaaagaact acataattct
     4321 actgctttta aggtcaaatt atttgttatc cttccagctt ttgctttgca aactgatgct
     4381 tctaatgaat ttggaattcg attgtctgcc aaccaagctg cttttagagt taataatcgt
     4441 gctgcttcgt gttctgcctc cattctataa atttccgcct ctactgcttt tacattatta
     4501 attggtgttg cataatctaa attaatacta tcttctaaaa gttctttggt tctttcgata
     4561 gttgcttctg cgacaccatt cgccattgat gcaacaaatg gtctggtatt atcaaaagtt
     4621 tccattacgc caccaaaacc tttttttaca tcaatttctg gagaacctaa tagattttca
     4681 gcaggaactc tacagtccgt aaaaacgatt gcagctgtat ctgatgcttt tatacctaat
     4741 tttttctcta agcgagcaat ttccattcct ggcgttcctt tttcaactac aaatgatttg
     4801 attgctgctt ttcctattgc cttgtctaat gttgcccaaa caactacaca atcgcatctt
     4861 tctcctgaag taacaaatat tttttcacca tttaaaatat aatggtcgcc atcttttact
     4921 gctgtagttg taatggctga ggaatctgaa ccacaattgg gttctgtaat tgccatagct
     4981 gcccaagatt ttgaaaatct ttttaattgc tcaggattcg caaccgcact gatggctgca
     5041 ttacccaaac cttgccctgg aatagtcaat aataaaccaa catcgccata acacatggtt
     5101 tgtatattaa tacagtttgc catattgcct ccatttttca cttcgccagc ttgtacttct
     5161 tctttttttg aacttgcacc accaactgct cctcctgaac cttcgttcat gccatttaac
     5221 cctgctgcaa acaaatccaa ctcctttgga tattcatgtt ctgctaaatc atattttctt
     5281 gatattggtc taaaaagaga ttgtgctgct gttaatgcaa acccttgaat gcctttaaac
     5341 tttttcggaa tttctaaatt cattatattg attatttgaa atatttaagt aattattttt
     5401 gttcgatgtt attataaatg caatccatta taacaaatgg caacggctct caaatctctg
     5461 taccagcgtt ctactggaaa atcttttata aaaccatgcc cacctaatag ttgcacgcca
     5521 ttggtgccaa tttgcataga tttgtcgcta cataaaacat gtgctaaata ggcttctttt
     5581 tgaaattcta agccttgttc tgcacgggca actgctcttt gtgttaaaat tcgcatacta
     5641 tcaagttcta ttttaatatc tgctatcata aaggcaacgg cttgtctatt actgattggc
     5701 tcgccaaatg catatctatc attggcataa gtaatcacat aatctaaaac tgcttgtgag
     5761 caaccaacag ccaaactaca ccatcctaat tttgaataat tgataaactc ttggtaattt
     5821 acacctttgc ttccgcccaa cattgctgat tcatctattc gtacattatc caaaacaatt
     5881 tttcctaatt cagcagtttt caaacccatc gaaacatcgg ctgaagtgct tacaccatca
     5941 gcagttctgt ctataaaaaa taattgattt tttccacctt ctgtttgtgc agctactaaa
     6001 aaatattgtg cggattctgc aaatgttacc atatttttct ctccagtgat aatatattgg
     6061 ttgccatctt tatttgcttt agtttttaaa gtctgcggag aaaataaagc ggttggttca
     6121 ttaatagcga tagatgcttt gggggcatta tcttcaacaa aatcctttaa taaaatttgt
     6181 tgctggctat ctgttcccca ctgtacaatg gcattgatta cactatttgc tgaaaataaa
     6241 gcaaatgctt ggcttaaatc gccataagct aaagtttcaa ccatcatcat ttgtgtggtg
     6301 gtggattttt ctttcatgat accacctaat tcttctggaa tttgcataaa tgccaattgc
     6361 aattcattgt attctcttaa aatctcttct gaaatcttgc cattataatc atctttctca
     6421 gcatggatac ggattttatc tgccaattgt ttaatagatt cttgaatcat caattgttct
     6481 tcattgtaat tcaaatcaaa caaatctttg gcttttttag gttctaatcg ttttgctttt
     6541 ccagaaccaa aagtttgaaa aacttttgta gctgcggctc ctgctttgac accgttttta
     6601 gccccttcgt acacaatctt ttcagataat ttacgaagct gtaatgtttt accaattttt
     6661 gaatctaaga gattattgta gaattcaaaa taactcgaaa agtctgtttt tttagcttct
     6721 tttttcttac tcattttagc gaaatttata tctaactata aatataactt taaatcagta
     6781 attctattat ttcaaaacgt gattttatct tttgtatatt gactttatta gtttttattt
     6841 tgttgtctaa ttcggtctaa atcatctaat gctgctggat aaatctcttg ttttatccaa
     6901 ttgtttttat cgtttctata ttctttgaat ttatatttta tagtaactgc caaatcgtaa
     6961 tcacttacag attgcataaa ctcataaggc atgtttaagt aagtaataaa gttgtcgtag
     7021 tcgtcatcag gtaaattgat aatatcgtaa gcattatata gcctaaataa ttcttttaaa
     7081 acagaacgtt ttgaaaattc tgtttgaagg tttgccgcca atcttttctc cttttctttt
     7141 cttgaaatga gttcgtataa ataagagatt gggttttgaa aaagtattgc atttggatat
     7201 aaatcagtat tgggaacgta taaattatta atcgattttc taatttgctg atgtgttttg
     7261 atggctgtaa tctgtacttc tgtcaattcg ataggtacat ggaacattct aaccaatatt
     7321 attttatttt ggtcaaacat cgtatcattg acgattaatt tttgaggtag aaacccactt
     7381 ttaaaaagca aaagcgtgtc cgacatttga gtatttgtag taaaatagcc atatttatct
     7441 gcagcaaaag agagttgcaa tcttctattg gcaatatcaa catgggctaa aggaatattg
     7501 ttttcatcaa caatttttgc atttagaatg aattcctttt gttgagcaaa aatcaattga
     7561 tgagtgatta aaagaaacac aattccacag agcaaacgca tattcaaaat taagaaaatt
     7621 gaacaaagaa ttttatattt gatttgttaa ttattgttgc ttatctttaa aatatggaga
     7681 taaacaattt agatgacgaa tctttgattg atttatatca aaaacacaaa gataatgatg
     7741 caattggcat actttacaaa cgatattcga tgttggtatt tggtttggca ttcaaatatt
     7801 ttaaacagga agatgttgct aaagacatgg ttactgaaat ttttatgaag gtaatggata
     7861 agctatctca agcaaaaact caaaaacctg aatcatttgc caaatggcta tatattgttt
     7921 ctagaaatga aataataagt gttcttcgta agaaaaaaat tcaagttgat accgttgatg
     7981 aaataaattc agaaaaattt atggaatctg aagaagaaat acgtcctatt atcaaagata
     8041 tagacgaaaa gcagttagac gctgcgatga gcaacttaaa cgaagaacaa aacaaatgta
     8101 ttcatttgtt ttacttacaa gaaaaatcct accaagaggt tgcttcctta actggctacg
     8161 atatcaagaa agtaaagagc tatattcaaa atggcaaaag aaatcttaaa atgtttttaa
     8221 ctaaaaaaaa ttaaaagcta tgaaaaagga agaattaaaa aaatacttag aacaaacact
     8281 gtcagataaa gaacgtaatg cattagaaca agaaatgcaa gacaatccat ttttaaatga
     8341 agctactgaa ggcttagaag catggaccca acaaggcaat gccgattttg atgcattaga
     8401 gaatgaatta aataaaaaca ttgatgataa gatagattcc aaaaatttag aaataaagaa
     8461 agttgtccga atgcctattt ttagaattat agcagttgca gctggaatta ttggtatatt
     8521 gtttcttacg gtaacttatt tacatcagca aagaatggaa acagaggctt tgtttgaagc
     8581 taattttaaa gtattaactc aaattgatgg tgcagtaaga ggtgaagatg cagaaattga
     8641 atcaaacatg aatgaagaaa aaaaggcagt ttattattat gagagagaaa attataaaga
     8701 agcaattgta cattatgaaa aagctttaca aagtcagcca gacaacaatc aaatgcaatt
     8761 atatttggct gtagcatatt tggctaatta tcaagcgaca gatgctattc aaattctttc
     8821 agagaatgat ttttctaata gtcaatataa acaagataga aattggtatt tagcattagc
     8881 ttatcttaaa aataaagatt taaaaaatgc caagccatta tttgaacaat tgagtgcctt
     8941 tgataattac tatcaaaatt cttctaaaaa attgctaaag tctttagaag gaaaggtatt
     9001 ataatcttta caattttacc acttgttttg aggtaaatat gccgtcttgt gcaattgtaa
     9061 taatataatt ccctgcaggt aaatctccaa tattattgat ttcaataaga ttcacacctt
     9121 tgttggctct cccaaaagct attttatctg ctactaattc tccagtcaag ttttgaatat
     9181 aaacatctaa aactgctgct ttatctaaac tcacttccaa ggtaacttgt tctgaaaatg
     9241 gattaggata aacttttaag cttttgatat tcttgttttg tttaattcct gatatagtgg
     9301 aagaatcttg ttttactatt ctgaatttat caaaaagtac tccatcaagg gtgtaataag
     9361 ttccagttaa ttcatttcct tttacttcta gaatcatact tccagcacca ttatcgcctg
     9421 tatcatcata ttttgtggta aagacaggat gcattttacc attgtgttct tcgtaatttc
     9481 cactattgcc aataacagag taaaccgttc cttttccttt caatgggcca taagtatatt
     9541 tggtatatac attaccattg tctggattgc catctgtaga atctacaact actttttctt
     9601 tgttaaaata actagaacga gcataatgac cattgattag atacgaacgt tcatacacat
     9661 gagaatgacc actaagtaca acatctacac catatttttc aagaatggga acaatctttt
     9721 ctctagtatt ctgcataaaa atttgataaa aatcatctgt atcgtgtgaa ccttttgaat
     9781 aaggtggctg atgaatatag gctacaataa atttcttatc cgtacttttt atatcatttt
     9841 ctatccaagt tttatacgga cttcccaaat cccaaaaaat agaataagta tataattctg
     9901 aattgagtgc cataaaatgc acattcccaa aatcataaga ataataagct tcggttttgg
     9961 aaggaatacc tcccatttct gcatttctaa atacttctac attgttataa tatggtccag
    10021 tatgtttctc agcaggcttg ctttgattga cactattgta atcatggttt cctggaattg
    10081 gataggaagg taaaaatctt aatattgaat cgtaaccata agtacgacca aatacttttt
    10141 cttgatattc tacatctttg ccatcttgat aagcattatc acccaaccag agccaagcat
    10201 ctactttatt atcttttaca taagtttgaa accaatttcg cacacgcact tgtctgctat
    10261 tggctttacc aaaatctcca attacccaaa atctaaatcc agtagtatct ccaactgaag
    10321 gagctgcaaa aaaatggtgt tgctcatctc ctcctgccaa tacattaaca tcatctgttc
    10381 ccacagaata ataatatttt tctcttgcac ttagtccatc aacaagaaca atatggtctt
    10441 ttatattatg tgtatcaatt attgttctat ttaatgcatt aggtgcatac cctattttta
    10501 caactgcctg agtagaatca gaagttcgcc acataatttt gattgattta tctgtcaaac
    10561 tttgtagata gggtccacgt accaatgttg tttgtgcatt tgtagttata cttattaata
    10621 caaacaaaaa agcaattgaa tatttcatat agttaagttt tgttgttggc atattaggca
    10681 tttgttatta gtaatttggc attaggcact agtaattaga ggttaggcat tagtattcat
    10741 atttcatatc tttactcatt ccttgttcct attcactatt tactaattta ctattcacta
    10801 atcaatttac attttttcta attcctcctg cctatttact atttactaat tactattcac
    10861 taatcaaatt aaatatgtct ttctgcgtgg taagatgaac gaactaatgc accactttct
    10921 acatattcta ttcctttgcc caatccaact tctttataat gtgcaaacaa ttcaggagtg
    10981 ataaattcaa ttacaggtgt atgcattttg gtcggctgta aatattgtcc gagggttaat
    11041 acatcacaac catgagccac taaatcgtcc atagcttcat atacttcttc ttttgtctct
    11101 cccaaaccca acataattcc agttttggtt cttttaccat attccttagt gcgaagaatt
    11161 tgctctaaac ttctttcata ctttgcttgt ggtctaattt ttctatacaa actttttact
    11221 gtttccatgt tgtgagaaac tacttcttga ccagcactaa taatcacttc caatgcattc
    11281 caatctgctt ttacatctgg aataagtgtt tcaatagttg tatttggcga aactaatttt
    11341 atttgtttga tggtttcatt ccaaattttt gcacctttat ctggcaattc atctctattc
    11401 actgaggtaa tcacggcatg tcgtacgccc atcaatcgaa tggcatcagc gacacgtctt
    11461 ggttcgtctt catctacaaa gaatggtctt ccagttggta cagcacaaaa tgaacaagag
    11521 cgtgtacaca catttcctaa tatcataaac gttgcagtac cacgtcccca acactctcca
    11581 atatttggac aattaccact ttgacaaatt gtatgtaatt tatgagaatc tacaatctct
    11641 ttcacttttt tgtaattctc tcccattggg atttttactt tcaaccaact tggctttggt
    11701 gttcttttat gttcagtaac ttcattcata tcattttcct ttaaattttc ttcctatttg
    11761 aaagcctaaa taaacacttg ggaaaaccga cttgttattg actaacataa taggcacttt
    11821 attgaaataa acatcacaac tgaagcctac ttctaatgct tttatcaagc cttcattttt
    11881 agaccaatca aaagaaagac ctgttttaat atgtaatcca ggtcgaaact tcaaattcca
    11941 accctttcta aaacctgctc cgccaacaat tgcttcaaaa gtttgtccaa attgattgta
    12001 atgtatataa tttagataat aagtttcatt agaattatag gctacattaa ttgtttcaga
    12061 aaaggtatag gtttgttcaa caggattata agacaatgaa ttggtatcta taaccgtcaa
    12121 catatagggt tttaaaatac ctaaagaaaa gcctcctgaa taatgataaa ataaacgtaa
    12181 gccatttttc tttgcctttt cagcaattaa tattttttgt ccaatattaa aatgagcagc
    12241 aaatagatta ttttgttttc caaagaaata tggtttaaat tgcatatcac ttgaacctgc
    12301 ataaggagaa tgttgcttga tttgtgcatt atgtttgtaa taacccaatt caaattgata
    12361 caatattgct gaacgatatt tccgacctga agtaatatca atatgtacgc ctaacccatc
    12421 acttcgaatt ttagcaccta ccaaccacgt tctatttgaa aaagatgttt ttttagattc
    12481 ttgttgctct acaaaattat attgtgctaa tgaagtttgc acaatcaata aaaatattat
    12541 tatagaaaat actctaaaca ccttattttt tataaagtat aaagataact atttctagta
    12601 aaaataaagt actaaaaaac tttcgtaaaa atattaggaa ctcgtttgaa tatcatttac
    12661 aaagataaaa ctatgacttg cgtcgtatat tacctttttg ttttccaaaa cgattaattg
    12721 tggactctga tgttgcacgc cagttttttc agcaatataa ttagaaatag ctctatgatt
    12781 taataaatct aaataatata ttgggaatga atgcgtccat tctttttcta ctctattttt
    12841 tgctgtacta cttacaggac aacgtgtaga atgcttaaaa actgcaaaaa ctttttgatt
    12901 ttctaataat tcatcaaatt gttcaatact gtttaagttt atccaatcca tctaagatat
    12961 attttatttt tggcttgtat ggaatgctcc aggacatcct gattttttac tctgacaaga
    13021 gctacttaat aaaacaatcg cacaaattgc aataagaatt gtctttttca tatccttaat
    13081 tttaattact taacgtacaa atttaaaaat ttatttataa actataataa aaatacaatt
    13141 attgtaatat ttttaacaca agttttaaac atggcaaatt atcattttat agcaattggc
    13201 ggtgctgtta tgcaccaatt agcattacat ttgaaagctg aaggcaatga ggtttcaggc
    13261 tcagatgata ttatctttga acctgcaatg acaaatttaa agcatgcagg tattttgcct
    13321 acatcatttg gctggtatcc agaaaaaatc aatcataata ttgatgctgt aattttggga
    13381 atgcatgcaa aggctgataa tccagaatta attaaagcag aagaattagg cttgaaaata
    13441 tattcatttc ctgaattttt ctaccaacaa tgtaaagaaa aaacaagaat tgcaatagct
    13501 ggaagccatg gaaaaacatc tatcacttca atgataatgc atattttcaa aaattatgac
    13561 aagcgtttcg actatctggt gggtgcgaaa ctagatggtt ttaaatatat ggtaagtaca
    13621 tcaaaagaca atgattatgc gattattgaa gcagatgaat acttaacttc gccattagat
    13681 ttaagaagca aattcttaca ttactttccg caaatagcta taatttctgg tattgcttgg
    13741 gatcatatca atgtttttcc aacttttgat aattacttag aaacatttag aaaattcatt
    13801 gccagcatac aacccgatgg ttatttaatc tacaaccaag aagatgagga gttgagaaat
    13861 ttagtacaat ctgtcgatac aaaacacata cagttaatac catattctcc gtttgatgca
    13921 gttattgaaa atgaagccgt aaaaattaaa tatgaaggac aatcctttcc aatagaagtt
    13981 ttcggcaaac ataatttatc taatttaaag gcggcttttg aagttggaaa attagcaaat
    14041 attgatgtaa aagatatctt aaatagtttt caaacattca aaggagctgc taaaagatta
    14101 gaatgtatat atcaagacaa catcaacaaa atcacaattt acagagattt tgcacatgct
    14161 ccatcaaagg taaatgcaac agtacaagct gttagagaaa aatattcaaa cagaaagatg
    14221 attgctttct ttgagttaca tacgtacagt agtctgcaag aaaattttat ttgtcagtat
    14281 gcacatactt tagatgtcgc agatgttcaa tttatatata tcgatgccga agctttaaaa
    14341 ataaaaaaca aagaaaattt cgacccaaat tggttaaaag aacaatttgc taatgataaa
    14401 attattgtct taaaagacaa ggcagatatt atccaacatt taaaacaagc tataacacaa
    14461 aactgtgttg tattattgat gagttctgga aatattgcta atattcaaat tgaagaaatc
    14521 ttacattctt aaactcttta cttcgttttg tgaatcacat ttgaagtggc ttgtgctgtt
    14581 ggtagaataa aaatctcctc tatattgaca tgagctggtt gtgttgccat aaaataaata
    14641 ctattggcaa tgtcttccgc ttgtaatggt tgaaagcctt catacacgct atcggcctta
    14701 tctttatccc atttaaaccg cactaaacta aattcagtat ctgccgcacc tggagaaact
    14761 actgaaactt taatattatg ttgcaccaaa tcgattcttg ctcctcttga aattgcacgt
    14821 acagcatgtt tcgttccaca atatacattg cctccaccat aagcttccca acctgctaaa
    14881 gatgctacat tgataatatg tccttgattt cttttaatca ttaaaggagc aatttctctt
    14941 gtcatatata ataatccttt gacattggta tctatcattt gttcccaatc atcatacaaa
    15001 ccttcttgaa taggaccttt tccagcagcc aatcctgcat tatttattag cacatctatt
    15061 gcttgccatt tctcttctaa actattaatt gcagatttca cttcttctaa gtttctgaca
    15121 tcaaaacata aagaaagtac atcaatatta tacgtagatt ttagattttt ttctaaagat
    15181 tccaatcgct cttttcttct tcctgtgatg attatattcc agcctgctgc tgcaaatttt
    15241 attgctgttg cttttccaaa ccctgctgtt gctcccgtta ttaaaattat atttgccatg
    15301 tacaaaggta aaaaatagct attacaaatc agtatttaat tgttattctt tgtataacaa
    15361 ataggtttca cagtcatcaa acaacttttt tttcttaaat ccgtaagctt gtggatttgc
    15421 cataaattgc aaccaagtac tatctttact taaacgtcta tcttcagtca aaaatttagt
    15481 ttgtaaaata taatcaactt gatgtgtttt catctgttct ataaatgttt tattattatc
    15541 atagtcgtat tctgcactaa aatcagtaaa gtttttatca atcatcaaac taaaacttaa
    15601 tacattacta aaaatgacat gaggtttatc tgtgtgttga ttcaggtctt taatcacttc
    15661 cttgtaggat tgtgttggac attcatcatt cgtcaataac ttgggtgtat tatatttttt
    15721 aatagatggc attttataca taaacagtgt aaatacaaac aaaacaatac ctatcctaat
    15781 tttatatcct tttgctagat tatctattaa tattgcaaaa acaaaactta aaatgggcaa
    15841 taaaaaaata ccataatgca tacgtggaaa gaataccaaa gaacttataa tactaggaaa
    15901 aataaaaatc aacaacacta aaaataaata tttattcgat tgaattgcat tgaatacatt
    15961 aagtctttgc ttcttgctaa tgagtgtata taatataaat aatacaacaa gcccatagcc
    16021 taataaaaca attttattga aaggtaataa ttgaaatgga aaaagaatat ccgtacttat
    16081 cgaaataatt aattgcaaat aaaatccaat atttacaaac attcctttta ttacgaccaa
    16141 aggaaaattt atcacaatat ccgtaaatgt attacagcca ggaaattggg ttttgcttat
    16201 tgcaatccaa tctataaatt ggttaaaatg tcctttattg aaaataatgt ttttaatagt
    16261 ataatgctga caaaatgcaa tataagctct atcaattccc ttaaaagaat ctgaaggcaa
    16321 acgaaaaaca gcaaaaatta aaattgcaat tgcaacaaaa ggtaaagcaa aaacgatatg
    16381 tttttttaga ctctcatttt ttataatata aaacaaagta aatgctgata aaatcaggaa
    16441 agcaagcatc aattctggcc gagcaaatgc cgcaataaaa atagtaaaac acaataaaat
    16501 caactgcctt gatttagact ttatccataa cttattgatg attaaaacaa ctaaaaataa
    16561 actcactaca acatgtgaaa ttcttggata ggttaaaata tttgttgtgg atataagtat
    16621 tgaaaaacct atccaaatac taataaaaaa cgaaatgcca taaacataca agaaagcaaa
    16681 taaacaaatc ggcaataaaa ttatcactac tttatagttc aacaaataca attcaattgg
    16741 tctttgttca aacaatgaaa gcaatttata ccacatatta tacgatggtc cccaatcact
    16801 tctgatatta tgaaataatt ccataccata acgcatatat tgcacctcat catcaaatga
    16861 aatatctacc aaattattga aattcctaat caatcttaat cctgcaatta gtatcaatat
    16921 aatacccaaa aacaaattgg tgtaactaaa tggttttagt ttatctttat aaaaaaaagt
    16981 ttgcagttca aaaggcataa acttgtacaa aataataaaa ttagctaaaa tagtcattta
    17041 attccgaacc ttaaatatca ataacacatg accatacaag aagcacaaaa ccaaattgac
    17101 caatgggtaa aaacttatgg tgttcgttac ttttcagaat taacgaatat ggcaatatta
    17161 acagaagaag ttggcgaggt tgctcgaatt attgcccgtc agtatggcga acaatcattc
    17221 aaagcctcag aaaaagacaa gaatcttgct gatgaattgg ctgatgtact ttttgttatt
    17281 atgtgcttag ccaatcaaac tggcattaac ctaactgaag ctttacaaaa gaatttagca
    17341 aaaaaaacac aacgtgatgc tgaaagacat ttcaataatg aaaaactcaa atagttttct
    17401 tttcagagat tatttgagaa ataaaaaata agccagctcc gaacaataaa gtaagagctg
    17461 cacctaataa cccgtgattg atatacggaa ctggaattaa ggtaacccaa gaagtacttt
    17521 gtgctactaa aaacaattca tttaaaacaa atccaaagaa gaaataaacg aatgccaact
    17581 tagctaaatt attggcttta agataattgt tatacaatcc ataagccaat aaatacatac
    17641 taaaagcacc caacaaaatc aaatgtaggt aagcaattat aattggtctg caactaaaaa
    17701 ccatatcgcc aactgtttga ttagaagaca atacttgcaa taaaaattta aaggttaatg
    17761 ctgcggctgc aaaataaaag aaaatataaa aactggcata tttattttta aaatctttga
    17821 ggtatttcca tattttagat aacattaata cccaagcaat aaattgtgca aaggcagcta
    17881 ttgtcgctac cacaagcaac aatttaggta aatctatcca taagatggat aatacataag
    17941 ttggaactat tgttattgca aaaacactta aatatttatt caaacttggt actgatttag
    18001 gcaacattgc gaccaaaaga gccatacttc caaagaaaaa ccaaccatca tattgatggt
    18061 gcaagtaaaa ataaatagag ctatttgtta aatctctatt catatttttt gtagccatta
    18121 aataagctaa gaaaaatgga ccgattgatg ataatgcatt tatcaataaa ccgacaattg
    18181 cccaaggttt aaatgttgtt cccttcggga tttgtttact atccaatata aaaaagagtg
    18241 cataaaaata agaaacaaaa atcgatagtg tagagaatgt aatggatatc gctttataac
    18301 cttgagctga aaatgccacc aacattccat aagctgaaat caaattgaca acaattaaaa
    18361 ttttatattt ttttagtttt tgttcatcta tataagtcgc aattaaaatc gttaaacctg
    18421 aatataaaat atgactaacc caacctgcaa acgcaaaatg agaatgtgca tgtaacaaaa
    18481 aagattgatt gaaaaaagga aagctatgtg ctgttttata acgcattaaa gcaccaaatg
    18541 ccgccaccaa cattaaatta aaaacaccta tcttaaacca cttttggcga cttcttatat
    18601 ctatcattga aaaaaacttt aaattcctaa aagcttatac aatatctaat gtaaaaatag
    18661 gtatctcaat tagcctaaac attaccaaaa ctttattttt gacaaaattg tcttttttta
    18721 aatgtctcct aattgtggtc taataactaa ttcttcgacc acactatatt ttgaaagttt
    18781 ggagattgaa aataccaggt ctgcaatatc atccacagga ataaaacgtt gttcaggcaa
    18841 atcagttcca ttccaagaat ccgttaatgt tgctccagga tgtatggctg taactttaat
    18901 atgcttatcc ttcaattcct ctctcaaact tcttgaaaag cctagcaaag catgttttgt
    18961 aattgcataa cttcctccac tagggtaacc ttttgtacca gcaaccgaac acatattgaa
    19021 aatttgccct gattgatgtt gtaacatatt aggcaaaata gattttgtaa gccaatatgc
    19081 agacaataaa ttagtttgca acatggtttc taaaaaaata tcctcttcat tagccatttc
    19141 gccacccaaa aatacacctg cattgttcac taaaacatca acttttttaa cctgttgaaa
    19201 aacaaaatca acaaattctt gtaattcttc tttatttgaa acatcgcatt ttttataaaa
    19261 gaatttatcg ttcaattcat actttttaag ttcgttggat aattcctcta aatgctctaa
    19321 agatttgcca caaccaatta cggtatgatt ttcctttagg aatttgatag caatcgcctt
    19381 accaatacct ctacttacac cagaaattac gatattcata actatttttt tttgtcataa
    19441 aaataaatta tttttacgct taattcaaat ccgatagatt taatatggca gaatatgttt
    19501 atgatagtgg cggtccttta tttcacgtag gcagatatgt gttgatggta aagagaagta
    19561 tcgcaaaacc agaaaaagcc attatgtatt ggaaagaaac tttaagacaa atgtacaata
    19621 taggcgttgg gtctgttgtt attatcttat tgatttcatt ttttattggt gcagtttctt
    19681 ctttccaatt tgcttataat ctttccaaat ctatggtgcc tatgtattat gtgggttatg
    19741 tagtgaaaga atctatgatt ttggaattag caccaacagt ttcttgtttg attttagcag
    19801 gaaaagttgg ttcaaattta gcttcagaat taggaacaat gcgattaact gagcaaatag
    19861 atgcttatga aattatgggt gttaatactt tttcctattt aataggtcca aaaatattag
    19921 ctgccgtaac gatggtgcca tttt
//
LOCUS       DAOQCW010000038        19480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1009_length_19480_cov_5.324891, whole genome shotgun sequence.
ACCESSION   DAOQCW010000038 DAOQCW010000000
VERSION     DAOQCW010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 19480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19480
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1009_length_19480_cov_5.324891"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PLS16_04970"
     CDS             <1..373
                     /locus_tag="PLS16_04970"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HNJ60463.1"
                     /translation="NQFISDIASGRNLTEEEVSEIAKGRIYSGEDAKSIGLVDEIGIL
                     KDAIAEAKKLAKINEPKVVYYPEDKKGFDLFLNSILGDESAKTKIFSLFQSEQKMYKE
                     IIQLKEYQQPQTRMPLVFKIY"
     gene            complement(360..1967)
                     /locus_tag="PLS16_04975"
     CDS             complement(360..1967)
                     /locus_tag="PLS16_04975"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNJ60464.1"
                     /translation="MMNNREIASIIQLLGNLMELQGVPDFRTKSYHFAARSIKSLEQE
                     LVDMPQETIENIPGIGKAISSKIIELISTGNLQLLETYLQKTPAGIIEIFQIKGIGPK
                     KIKLLWEELDIESIGELLYACNENRLITIKGFGVKTQQNIKEQIEFIQKNASYFIWAN
                     LEPLVNEILVEIKEQFPKLLIETVGDFANKKIVLEKITFLIGTEQLQIIEQLQQQFIQ
                     YPIEFISCTPSKFYLKRIELTTTEDFDILKKSMANQQEFSIETAVYESAQIPFIAPEF
                     RDIANIHQLAKEGLLNNIIELKDIKGIIHTHTKYSDGSNTIEEMAQHCMHKGFEYLVI
                     SDHSKSAFYANGLSVERILEQHQEIDQLNLKYPNFKIYKSIESDILYDGSLDYEDNVL
                     QMFDLVIASVHSNLRMTEEKAMERLIKAIENPHTTILGHLTGRLLLSRKGYPINHQKV
                     IDACAENDVVIELNANPNRLDIDYKWIDYCQQKNVKIAINPDAHNLEGIADVRFGVIA
                     ARKGLLFKHNTLNTLSQSEFDDFISKS"
     gene            complement(1957..3474)
                     /locus_tag="PLS16_04980"
     CDS             complement(1957..3474)
                     /locus_tag="PLS16_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60465.1"
                     /translation="MNAQSVTKADALMKDYKYSEAIIELKPIADKGNIQAIRKIAECY
                     RKINDYVNAEKYYSIVVVDKNSIPKNYLYYGQMLMNNNKYKEAQQWFNSFLNSKPSVK
                     DSTLAATLLTSCLKNLGETTPIRKVKLYNLEALNSPAADFCAVAYGGDKIIFTSSRSG
                     KKNSFDGQGYAQVYIASSLNDTQYSVAPVSGTINSKNFNSGPVSINTQKSKIYYTKNN
                     IQYGDAISNKKDEVTLKIFEAKFDGTSAKDFSEMAFNDAEYSCAYPAINKEGNLIYYT
                     SDRAGGFGGKDIYYSTFIDGKWSKPKNAGNIINTPGDEKFPFVHDDGTLYFSSNGHQG
                     LGGLDIFKAMINDSGRVINIMNLGLPFNSSADDFGFYLDSNYHKGFISSDRVNGKGSD
                     DIYAFQYETIPFYLEVFSDGNLQDSVAVVLNYKSISDTVFTDKSGIVQLELLPFTLYK
                     IVIAKEGFVHQEIKIKTSDIFKPLERKVYLELKTEKVEQKTPINEVELLEQKNDE"
     gene            complement(3525..4361)
                     /locus_tag="PLS16_04985"
     CDS             complement(3525..4361)
                     /locus_tag="PLS16_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNJ60466.1"
                     /translation="MKLYTINTGYFKLDGGAMFGIVPKSMWQKLNPPDENNMCTWALR
                     CLLVKDKDRLLLIDAGIGDYLNETYRQRFYPHGDETLEQSLAKHGFKPEQITDILLTH
                     LHFDHCCGVLKRDENNQAQLRFPNATIWTSFQQWEWAMHPNAKEKASFLEEILMPIKN
                     LGKLKFVENEEFQMENVELEFVNGHTESMIICHIYKGKHTITYCADLLPSHHHIRLPF
                     VMAYDIRPLDSLKEKHFLLERAYENDHILFFEHDKDIEACTVKKTAKGIEFDKVIQIS
                     DL"
     gene            4449..5840
                     /locus_tag="PLS16_04990"
     CDS             4449..5840
                     /locus_tag="PLS16_04990"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HNJ60467.1"
                     /translation="MDFIKQTIDKNKDRYIFELMEWLKIPSVSADPKFEAGVLEAANF
                     LAKQLRSISLSNVELLPTKGFPVVYAEKIIDKDLPTILVYGHYDVQPADPYELWNSPP
                     FEPVVKKTDIHPDGAIFARGACDDKGQVYMHVKAIEAMLENNALPCNVKVIIEGEEEV
                     GSANLENFLIDYKDKLAADIILVSDTGIINNDTPSICNGLRGLCYMEIEVTGPNRDLH
                     SGIYGGGVDNPLNVLAHIIAKLKDENGRILIDNFYDEVLAVSDEERKQINAVPISKKE
                     FEGSIGLKEAFGEIGYTLHEQLTIRPTLDVNGIWGGYIGEGAKTVLPSKAQAKISMRL
                     VPNQSPDKIAELFKTYIEKITPNTVQVKVTTHHGGEPYLTPVDSPAFIAAEKAMEQTF
                     GKKPIATRNGASIPIVALFEKILGFKTVLMGFGLDSDAIHSPNEHYGLFNYFKGIETI
                     PYFYKNFTELNKK"
     gene            5860..6852
                     /locus_tag="PLS16_04995"
     CDS             5860..6852
                     /locus_tag="PLS16_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60468.1"
                     /translation="MYFKHQTLVLNIPLIIIGFFLLNRGAGMLVDGASSIARKFKFPE
                     TMVGFTIVALGTALPKFFTNIIAVMNGYNDLFLGNIIGSGIVNLLGIIGLVGLNRSMR
                     ATRYTVFIGFPIAILSTFLVYILSNNLFLFKTNEATPDLDFNDGIYLLIAFVLFMTYA
                     YFNVKKNHTIWFTKDTEEPEFYPFVFAVILVVVGVVGSYAGAILVVDNLIDLSRKYDI
                     SQRFLGQFILSVGGSLAMFWWTKNFKINQTRFEISQLLGTNLLYLLGILGTCAIIAPI
                     PFNIRYNLDLFLLIIANIVMILLLVIGRKLSINLWKSKVLVFLFLVYITYLFVR"
     gene            6941..7789
                     /locus_tag="PLS16_05000"
     CDS             6941..7789
                     /locus_tag="PLS16_05000"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HNJ60469.1"
                     /translation="MKTLSFYFVMLLTTSSLLFTSCKSNAEAGYTSDSLGLESVSKNA
                     TESYAPNSASQSSENSSSSTIPIERKLVKEADLSWETNNISKSHQLLLEKVKKYNAYI
                     SNDSQSKDDYQISNSMVIKLPADKFDAFINELQSNVKNFDSKNIRVLDVTEEYIDVEA
                     RIKTKKELEQRYTEILKRANTVEDIMSVEAQLNSIRAEIESVEGRMKLLNQQITLSTI
                     NINFYETTSAPVGFFGNIGKSIIEGWNSFLHFILNLISIWPTILILGGVVYFIYKKIK
                     QRKSSV"
     gene            complement(7828..9174)
                     /gene="nuoF"
                     /locus_tag="PLS16_05005"
     CDS             complement(7828..9174)
                     /gene="nuoF"
                     /locus_tag="PLS16_05005"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNJ60470.1"
                     /translation="MSRKILLENEHIEGIDTLEVYRKNGGYATLENTLKCLSPNDVQL
                     EVKNSGLRGRGGAGFPTGMKWSFLARPEGVPRYLVCNADESEPGTFKDRYLMERIPHL
                     LIEGMILSSYALGVNTAFIYVRGEFFYIIKILEKAIQEAREAGLLGKNILGYNYNLEI
                     YVQPGGGAYICGEETALLESLEGKRGNPRIKPPFPAVAGLYGCPTVVNNVETLAAVVP
                     IVKMGGEAYAKIGLGKSTGTKLISASGHINKPGVYEIELGLPVEEFIYSDEYCGGIRN
                     GKKLKAVVAGGSSVPVLPTELILKTASGEPRLMTYESLSEGGFQTGTMLGSGGFIVMD
                     EDTSIVKNLFTFARFYRHESCGQCSPCREGTSWMEKILHKMIDGDGKIEDIDLLWDIQ
                     SKIEGKTICPFGDAAAWPVASAIRHFRPEFEEYIRKGNKVENVRHYYRLNNLPVPV"
     gene            complement(9190..9714)
                     /locus_tag="PLS16_05010"
     CDS             complement(9190..9714)
                     /locus_tag="PLS16_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNJ60471.1"
                     /translation="MSTTTEKISFTPEVLQKVQEIIKRYPEGRHKSALLPILHIAQAE
                     FDGWLSPKTMDYVAEILNIKPIEVYEVATFYTMFNLEPVGKYLIEVCQTSSCWMCGAE
                     DVVAYLENKLGVKVGEITSDGMFQIKLAECLGSCGTAPMFQIGDKYYENLTFEKIDVI
                     LEELRKENKRSRYC"
     gene            9794..10783
                     /locus_tag="PLS16_05015"
     CDS             9794..10783
                     /locus_tag="PLS16_05015"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNJ60472.1"
                     /translation="MKKYVFIFGAGRTGTTLATRIIEKSEKVALFKQESHAYLAFWKW
                     GNQLQIPSKIKTPNDFADYVLERFPAINFGWKMENEYPKLLKLVEQIKNEQFFPRDTN
                     DFLNFLFKQIPNENNKVEYIGEKTPAHIYYYQEILNYFKPAKVIITIRDPRATAYSEL
                     VKKNIKALGLAPFNILTFIARFNTVYYLVDAIKNKIGKENVLLVRYEDIVLQPEATIQ
                     QICSFMQIEFQEDMLQMGVFNSSFGDKFQKDKNFNTENIDRWQTELDSSFIAQIEKNC
                     KNIMQKYNYPISNLGNGSDLKLTAKLKLFVAKYFVQYFPALFHYLNKHEKYRL"
     gene            complement(10808..11857)
                     /gene="queA"
                     /locus_tag="PLS16_05020"
     CDS             complement(10808..11857)
                     /gene="queA"
                     /locus_tag="PLS16_05020"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNJ60473.1"
                     /translation="MKLSQFEFELPKEKIALYPNKNRDECKLMVVHRKSGKIEHKIFK
                     DVLNYFDDGDVMIVNNTKVFPARMYGTKEKTGAKIEVFLLRELNHELRLWDVLVDPAR
                     KIRVGNKLYFGDDDLVAEVVDNTTSRGRTIRFLFDGTDEQFRAKIDLLGNTPLPKYIK
                     RDPEELDKEYYQTLFAKEEGAVAAPTAGLHFSNELIKRLEIKGVDFAEVTLHIGLGTF
                     RSIEVEDLSKHKMDAEYVKVESKAVDIVNKAKQKGKNICAVGTTSMRTIESAVSAEHL
                     LKPMEGWTNLFVHPPYDFSIANSMITNFHLPKSSLIIMVAAFAGYDLTMEAYEQAIKK
                     KYNFYSYGDAMLIID"
     gene            11953..13206
                     /locus_tag="PLS16_05025"
     CDS             11953..13206
                     /locus_tag="PLS16_05025"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ60474.1"
                     /translation="MFRILGDTILISLQLFWSNKTRTFLSLFGISIGIFSIVSILTAV
                     DALRGNINDSIKSMGDNVLFINKWAWTFDGTSNYPWWKYAQRPEVKYNIYKHISSKTK
                     LGAYSAFEVNPRSTPEISYKSRKTRKAMISAVTADYAKVFDLDILHGRFLSSMEANNG
                     VNVCIIGKDVADKLFKDIENPVDYELKIDGRSVRVIGVLNKKGESLLGFNFDKSIILP
                     YTYYNNNFSINEKNTDFTVEVRTKDGVSLDELAQELTGLMRAARKLRPVQADDFTINQ
                     LSVIAKGFDSLFSILGIIKWIIGGFSLLVGGFGIANIMFVSVSERQSIIGIKKALGAK
                     NSDILLEFLFESIILCMIGGGIGLLLVWAITGPASEASGMQFVLSAGNILVAISVSFI
                     LGILSGIIPAYIAAKMDPVEAIRSK"
     gene            complement(13441..13842)
                     /locus_tag="PLS16_05030"
     CDS             complement(13441..13842)
                     /locus_tag="PLS16_05030"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNJ60475.1"
                     /translation="MKTNTSTGLLILRLTIGTLLLVHGVSKLYNGVGWISDMLGSPGH
                     ISPVAYLVFMGELFATVLLICGFRTKIAAMLIIINMCAAIFLVHRNEIFTFSENGGWG
                     IELPALFLFGAITLFFAGGGEYAASNESNWD"
     gene            complement(14061..14462)
                     /locus_tag="PLS16_05035"
     CDS             complement(14061..14462)
                     /locus_tag="PLS16_05035"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNJ60476.1"
                     /translation="MKSNLNLGLLILRLSIGILLLLHGVSKLQFGIDWMVQMLEAKGI
                     PGFIAYGSIIGEVLAPLMIIFGFRTKIGALIIAINMATAIFLVHSADIFTLSDKGGLT
                     IELPALFMLGALALVFSGAGKFAASTSSKFD"
     gene            complement(14508..14900)
                     /locus_tag="PLS16_05040"
     CDS             complement(14508..14900)
                     /locus_tag="PLS16_05040"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNJ60477.1"
                     /translation="MQIRRRKEFHDEDAMSAMNDIMFFLMLFFLIISTMANQNIIKLL
                     LPKAEETEQLAKQPVTLSVTEDLKYYINQSEVNVTDLEAALQSAMANNIDNTVVLRVA
                     KGLTVQDLVDVMQVGARLKIKMVLSTDK"
     gene            complement(14906..15610)
                     /locus_tag="PLS16_05045"
     CDS             complement(14906..15610)
                     /locus_tag="PLS16_05045"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNJ60478.1"
                     /translation="MTILNFILQVTTSVDSASAIQTVANTAPESISLFGLLTKGGIVM
                     IPLLILSVIAIGFIVERIMYVNQHSKIDVNLVNNVLDKLYSGNVQNAEALCIQSNSAL
                     GNVLQAGITQLGKPIDHIEKALETQSNIELMEMERHTGYISLISGIAPRLGFVGTILG
                     VIKIFYSISLSSDISIGTISGGLYEKMISSGTGLIIGIIAFMGYNFILTKIDGYSLKL
                     QKAVFEFTKGIKKPTK"
     gene            15723..17051
                     /locus_tag="PLS16_05050"
     CDS             15723..17051
                     /locus_tag="PLS16_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNJ60479.1"
                     /translation="MNKFLCFIMILLLFFQHKVIAQKLDNNRQEYFLNKNMQGSEYRF
                     SFVHITDVHIGEGFSDYGTYGFAKDTMPDMDNSKPTLSLIRAVQWINENYQAKNIKFV
                     IVSGDLTGKAQHSEFEKFKSIMSTLEIPYIPMIGNHDVWQYVRYGFEEPYPTGDTVIN
                     TIFKDAFESAKHFFDNWNDGTRLTATFDPESGHEHYFQNFSFEYDGFKFYALDFNPRY
                     HVKKEEPGIGPEAQLHDIPGGTFQWLKKELSLQPKHKENTILLAHHPLTDNLIYIIAG
                     FVFEYDEYDKLTKMLIPYSNNLALYLAGHVHIYNDYQIRSLNKLYNIMPARVMPASKD
                     FDSAYFQIIHVYKPQEDLTPIKTNNLNTNDVLIYPNPTSDLLNIQLKENIGAIDFSIL
                     DSEGNYILKTKANKIDQYNYTLSIGQLAKGNYQLKIDNENYSGSFKIQKQ"
     gene            complement(17052..17459)
                     /locus_tag="PLS16_05055"
     CDS             complement(17052..17459)
                     /locus_tag="PLS16_05055"
                     /inference="COORDINATES: protein motif:HMM:NF015180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerC domain-containing protein"
                     /protein_id="HNJ60480.1"
                     /translation="MENHKHSANKISIYLSLLCAIHCLAMPVFIAIMPFASVFLEKYH
                     WLELVVIFSTLLLGTNSIVHGYKYHHHDKLPAYLFGIGISILIFSSIIHLGFHSQSIL
                     LQGLNIIGAIFSAAGQIQNLRLSRSGAKKVVVA"
     gene            17544..17960
                     /locus_tag="PLS16_05060"
     CDS             17544..17960
                     /locus_tag="PLS16_05060"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNJ60481.1"
                     /translation="MKNIKERLKKSGLRVTPIREEVLEYFTDQQRALAHADIEANYKN
                     KFDRVTLYRTLTTFIESGILHKVSDNSGIAKFALCQHHDCNQHNHKDNHVHFKCNICN
                     KIECLHELSIPDFKLPKNYIMTTANLLIDGICSNCK"
     gene            17994..19142
                     /locus_tag="PLS16_05065"
     CDS             17994..19142
                     /locus_tag="PLS16_05065"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNJ60482.1"
                     /translation="MKSLFKTVIVIALVYVGLLFFGPKHIQNGLKYWFSNVDDYKVFT
                     NNTIQKSSSPKAWPISSDYNKKQLSAEDEKYMTDRKTCAYLVFQNGQLIHEKYYDDYD
                     SSTISGSFSMAKTVNALLIGKLIEEGKIGSINDDIKKYVPQLTQIKEGELTIKDVITM
                     SADIDWKESYWNIFSMTAESYYGEDLDHLMGKLKVRKDQQQGKIWEYQSCCTQILGYI
                     IKNVSNKSISAYTKEILWQPLGMENDALWSTDKNGGIEKSFCCLNATARDFGKLGALV
                     LQKGKVDSTQIIPAQWIADMTKPASYLKTADGRTCDWYGYQTWIIQQNNMSVPYFRGV
                     HGQFIFVIPEKNAVIVRLGKKIEKKNIDEHEQNDDIKAYLRFGLSLLK"
BASE COUNT         6844 a         3080 c         2768 g         6788 t
ORIGIN      
        1 aaaccaattt atttcagata ttgcatctgg tagaaattta acagaagaag aagtatctga
       61 aattgcaaaa ggtagaattt attctggtga agatgcaaaa tcaataggtt tagtagatga
      121 aattggcata ttgaaagatg caattgctga agcaaaaaaa ttagccaaaa tcaacgaacc
      181 aaaggtggtt tattatccag aagataaaaa aggatttgat ttgtttttga atagcatttt
      241 aggtgatgaa agtgcaaaaa ccaaaatatt ctctttattc caaagtgaac aaaaaatgta
      301 taaggaaatt atacaattga aagaatatca acagccacaa acaagaatgc cattggtttt
      361 taagatttac taataaaatc gtcaaattcc gattgactta atgtatttaa ggtattgtgt
      421 ttaaaaagca aaccttttct ggcagcaata acaccaaacc ttacatctgc aattccttct
      481 aagttgtgtg catctggatt tattgcaatt tttacatttt tttgttggca ataatctatc
      541 catttataat caatatccaa acgatttgga ttggcattta attcaatcac aacatcattt
      601 tcagcacaag catcaattac tttctgatga tttatgggat atccttttct tgataacaac
      661 aaacgacctg tcaagtgtcc caatatcgtt gtgtgaggat tttctatcgc ttttattaaa
      721 cgctccattg ccttttcttc agtcattctt aggtttgagt gtactgaagc aataactaaa
      781 tcaaacatct gcaaaacatt atcctcataa tctaaactgc catcatataa aatatccgat
      841 tcaatacttt tataaatctt aaaattagga tacttcaaat tcaattggtc aatttcttga
      901 tgttgttcta agattcgttc tactgacaag ccatttgcat aaaaagcact tttactatga
      961 tcggaaataa ctaaatattc aaaaccttta tgcatacaat gttgagccat ttcttcaatc
     1021 gtattgctac catcactata ttttgtatgt gtatgtataa ttcctttaat atcttttaat
     1081 tcaataatat tgttcaacaa tccctctttt gctaattgat gaatattggc aatatctcta
     1141 aattctggag cgataaaagg gatttgagct gattcataaa ccgcagtttc aatagaaaat
     1201 tcttgttgat tagccattga tttttttaaa atatcaaaat cctcagtggt cgttaattct
     1261 attcttttta aataaaattt tgacggtgta caactaataa attcaatagg atattgaata
     1321 aattgttgtt gcaattgctc aataatttga agttgttccg ttccaattaa aaaagtaatt
     1381 ttttctaaaa caattttctt attagcaaaa tcacccacag tttcaattaa taatttggga
     1441 aattgctctt taatttcaac taaaatttca ttcactaatg gttccagatt tgcccaaata
     1501 aaatagctag cattcttttg tataaattca atttgttctt tgatattttg ttgtgttttt
     1561 acaccaaaac ctttaatcgt tatcaaacga ttttcattac atgcataaag taactcgcca
     1621 atagattcaa tatctaattc ttcccaaagt aatttaattt tcttaggccc aataccttta
     1681 atttgaaata tttcaataat accagctggc gttttttgaa gataagtttc taatagttgt
     1741 aaatttccag ttgaaattag ttctataatc tttgatgaaa tggctttgcc tattccaggt
     1801 atattttcaa tagtttcttg aggcatatct accaactctt gctctaaaga ttttatactt
     1861 cttgcggcaa aatggtagct cttcgttcta aaatcaggta cgccttgcaa ctccatcaag
     1921 ttgcctaata attgaataat acttgcaatt tctctattat tcatcatttt tttgttctaa
     1981 taattcaacc tcattgattg gagttttttg ctctactttt tcagttttca attctaaata
     2041 aactttacgt tctaaaggtt taaatatatc actcgttttt attttaattt cttgatgaac
     2101 aaaaccttct tttgcaataa caattttata caaagtaaag ggcaataact ccaactgcac
     2161 gatacctgac ttgtccgtaa atacagtatc tgaaatactt ttataattaa gaacgactgc
     2221 tacgctatct tgcaaatttc catctgaaaa aacttctaaa taaaacggaa tagtttcata
     2281 ttgaaaagca taaatatcat cagagccttt gccattcact ctatccgatg aaataaatcc
     2341 tttgtgataa ttagagtcta aataaaatcc aaaatcatca gcagaggaat taaaaggtaa
     2401 acctaaattc ataatattaa taactcgtcc actatcattt atcattgctt taaaaatatc
     2461 taaaccacct aaaccttgat gcccatttga agaaaagtat aaagttccat catcgtgtac
     2521 aaaaggaaat ttttcatcac ctggtgtatt gataatattg cctgcatttt tgggtttaga
     2581 ccattttcca tctataaaag tactataata tatatctttt ccaccaaaac cacctgccct
     2641 atctgatgta taataaatca aatttccttc tttattgata gctggataag cacaagaata
     2701 ttccgcatca ttaaatgcca tttctgaaaa atccttggca cttgtaccat caaacttagc
     2761 ttcaaaaatt tttagtgtta cttcatcttt cttattggaa attgcatctc catattggat
     2821 attattttta gtatagtaaa tctttgattt ttgtgtattt attgatactg gtccactatt
     2881 gaaattttta gaattaattg taccagaaac tggagcaaca ctatattgcg tatcgtttaa
     2941 tgaactagca atatatactt gagcataacc ttgtccatca aaactatttt ttttcccaga
     3001 tcttgaagat gtaaaaataa ttttatctcc accatacgcc actgcacaaa aatctgctgc
     3061 aggtgaattt aaagcttcta aattatacaa ttttactttt ctaatgggtg ttgtttctcc
     3121 taaatttttt aaacaagaag tcaataaagt tgctgctagc gtcgaatctt ttacactcgg
     3181 ctttgaatta agaaatgaat taaaccattg ttgagcttcc ttatatttat tattattcat
     3241 caacatttga ccataataca aatagttttt aggtattgag tttttatcaa caaccacaat
     3301 agaataatac ttttcagcat ttacataatc attaattttt cggtaacact ctgcaatctt
     3361 tcgtattgct tgaatattcc ctttatccgc aattggttta agttcaatga tagcttctga
     3421 atatttgtaa tctttcataa gtgcatcagc ttttgtaact gactgagcat tcatttcaat
     3481 aaaataaaaa aaacttacta tcaatataaa tatacgcaaa tacattacaa atctgaaatt
     3541 tgaattactt tatcaaattc tatacctttt gcagttttct ttactgtaca agcttcaata
     3601 tctttatcgt gttcaaaaaa taaaatatgg tcattttcat aagcacgttc caataaaaaa
     3661 tgtttttctt ttaaagaatc caatggtcta atatcgtaag ccattacaaa tggcaatcga
     3721 atatgatgat gagaaggcag caaatcagca caataggtaa tggtatgttt tcctttgtaa
     3781 atatggcaaa ttatcattga ttcagtatgt ccattgacaa attctaattc tacattttcc
     3841 atttgaaatt cttcattttc aacaaatttc aatttaccca aatttttaat tggcattaaa
     3901 atttcttcta aaaaagatgc tttttcttta gcatttggat gcatagccca ttcccattgt
     3961 tggaaacttg tccaaatcgt tgcattcgga aatcttaact gtgcttgatt attttcatca
     4021 cgttttaaca caccacaaca atggtcaaaa tgtaaatgtg tcaataaaat atctgttatt
     4081 tgttcaggtt taaaaccatg ttttgctaat gattgctcca aagtttcatc gccatgtgga
     4141 taaaatcgtt gtctatacgt ttcattcaaa taatcgccaa ttccggcatc tattaacaat
     4201 aacctatctt tatcttttac taataggcaa cgtaatgccc aagtacacat attattctcg
     4261 tcaggtggat tgagtttttg ccacatactt ttcggaacaa taccaaacat tgcaccacca
     4321 tctaatttaa aatatcccgt atttattgta tatagcttca tatttaagtt tattttttct
     4381 ataattatta tgttaaagat agtagaattt aaagaattac gttaatttca aacaaaaata
     4441 gtgtgattat ggattttatt aaacaaacaa tagataaaaa taaagacaga tatatatttg
     4501 aattaatgga atggcttaaa attccatctg tttctgccga tccaaaattt gaagctggtg
     4561 tattagaagc agcaaatttc ttagccaaac aattgagaag tattagttta agtaatgtcg
     4621 aattactgcc taccaaaggc tttcctgttg tatatgctga aaaaataatt gataaagatt
     4681 taccgactat tttagtttat ggacattatg atgtacaacc agctgatccg tatgaactat
     4741 ggaattctcc cccatttgaa cccgtcgtaa agaaaactga tattcatcct gatggtgcta
     4801 tttttgcacg tggtgcttgc gatgacaaag gtcaagtata tatgcatgta aaagctatag
     4861 aagccatgtt agaaaataat gcattgcctt gtaatgttaa agtaattatt gaaggagaag
     4921 aagaagtagg ttctgcaaat ttagaaaact ttttaattga ttataaagat aaattagctg
     4981 ctgatataat tttggtttca gacactggaa ttatcaataa cgatacacct agtatttgca
     5041 atggtttacg aggtttatgt tatatggaaa ttgaagtaac aggaccaaat cgtgatttac
     5101 attcaggaat ttatggtggt ggtgttgata atccattgaa tgtgctagct catattattg
     5161 caaaactaaa agatgaaaac ggacgaattt taattgataa tttctatgat gaagttttag
     5221 ctgtttcaga cgaagaacgt aaacaaatca atgctgtacc aatttctaaa aaagagtttg
     5281 aaggtagtat tggtttgaaa gaagcctttg gtgaaattgg ttatacatta catgaacaac
     5341 ttactattcg accaacatta gatgtcaatg gaatttgggg tggctatatt ggcgaaggtg
     5401 ctaaaactgt tttgccctca aaagcccaag cgaaaattag tatgcgttta gtacccaacc
     5461 aatctcctga caaaatagct gaattattta aaacatatat tgagaaaatt acgccaaata
     5521 ccgtacaagt aaaagtaact acacatcacg gtggcgaacc ctatttgaca ccagttgatt
     5581 ctccagcttt tattgcagca gaaaaagcaa tggaacaaac atttggtaaa aagcctatcg
     5641 caacaagaaa cggtgcaagt attccaatag ttgcattatt tgaaaaaata ttaggattta
     5701 aaactgtttt aatgggcttt ggtttggata gtgatgcaat tcactcgcca aatgaacatt
     5761 acggcttatt caattatttt aaaggtattg aaacaattcc atatttttat aaaaatttta
     5821 ccgagctcaa taaaaaataa tccttagatt taagtacaaa tgtatttcaa acaccaaacc
     5881 ttagtattaa atattccatt aattattatt gggtttttct tattaaacag aggtgctgga
     5941 atgttggtgg acggagcatc atccattgcg agaaagttca aatttcctga aacgatggtg
     6001 ggctttacaa ttgttgcttt aggcacagct ttacctaagt tttttacaaa tatcattgcc
     6061 gtaatgaatg gctataatga tttatttctt ggaaatatta ttggctctgg catcgtaaat
     6121 ttactgggta ttattggttt agttggctta aatagaagta tgagagcaac aagatatacc
     6181 gtctttattg gctttccaat agctatttta agtacatttc ttgtttatat acttagtaat
     6241 aatttattcc ttttcaaaac gaatgaagct acacccgatt tagattttaa tgatggtatt
     6301 tatttactca tcgcatttgt attatttatg acctatgcct attttaatgt caaaaaaaat
     6361 catacaattt ggtttactaa agacactgaa gagcctgaat tttatccctt tgtctttgca
     6421 gttattttag ttgttgttgg tgtcgtgggt tcttatgctg gagcaatttt agtcgttgat
     6481 aatcttatag atttatcaag gaaatatgat atttcacaaa gatttttagg tcaatttata
     6541 ctttctgttg gcggttcatt agcgatgttt tggtggacta aaaactttaa aatcaatcaa
     6601 actagatttg aaatcagtca attattaggt actaatttat tatatctctt aggtatttta
     6661 ggaacttgtg ctataattgc tccaattcca tttaatattc gctataactt agatttattt
     6721 ttactaatta ttgccaatat cgtgatgatt ttgcttttag taattggtcg aaaattatcc
     6781 atcaatttat ggaaatcaaa agtattggtc tttttatttt tagtttatat aacttattta
     6841 tttgtaagat agtttgaatt gcttttattg atttttaaca atttgaatat ttttggtatt
     6901 tgttttttgt tcaaatttac atcaaccaaa atcaatcaaa atgaaaacat tatcttttta
     6961 tttcgttatg ctgctgacga ctagcagttt attatttaca tcttgtaaaa gcaatgctga
     7021 agctggctat acctctgaca gtttaggctt agaatccgtg agcaaaaatg caacagagag
     7081 ttatgcacca aattcagcgt ctcaaagtag cgaaaatagt tcctcctcaa ctattccaat
     7141 agaacgcaaa ttggttaaag aagcagattt gagctgggag accaataata ttagcaaatc
     7201 acatcagcta ctattagaaa aagtaaagaa atacaatgct tacatttcca atgactcgca
     7261 aagtaaagat gattatcaaa tttcaaacag tatggtaata aaattaccag ctgataaatt
     7321 tgatgctttt atcaatgaac ttcaaagtaa tgtcaaaaat tttgacagta aaaatattag
     7381 agtacttgat gttactgaag aatatattga tgttgaagca agaataaaaa caaagaaaga
     7441 gttggaacaa cgctatactg aaatcttaaa acgagccaat acagttgaag atattatgag
     7501 tgtagaagca caattaaata gtataagagc agaaattgaa tccgttgaag gaagaatgaa
     7561 actactcaat caacaaatta cattgtccac aataaatatc aatttttatg aaactacttc
     7621 agctccagtt ggtttctttg gtaatatcgg caaaagtatt attgaaggtt ggaattcatt
     7681 tttgcatttc atattaaatt taattagcat ttggccaact attcttattt taggcggtgt
     7741 tgtatatttt atctataaaa aaataaagca aagaaaatct agtgtataat agatattaaa
     7801 aagccctgaa ttaaatcagg gcttttatta tactggaacg ggcaaattat tcaatctata
     7861 ataatgcctt acattttcaa ctttattgcc ttttcgtata tattcttcaa attctggtct
     7921 gaaatgacga atggcactcg caacaggcca agccgctgcg tctccaaatg gacaaatggt
     7981 ttttccttca attttgcttt gaatatccca caataaatca atgtcttcaa ttttaccatc
     8041 gccatctatc attttatgta agattttttc catccaactt gttccttctc gacaaggcga
     8101 acattgaccg caactttcat gtcggtaaaa tcgggcaaaa gtaaataagt tttttacaat
     8161 agatgtatct tcatccataa caataaaacc gccagaacct aacattgttc ctgtttgaaa
     8221 accaccctca cttaatgatt catacgtcat caatcgtggt tcgccactag ctgttttcaa
     8281 tatcaattcc gtaggaagca ctggaacaga agaacctcct gcaacaaccg cttttaattt
     8341 tttaccattt cggataccac cacaatattc atcactgtat ataaattctt caactggcaa
     8401 acctaattca atttcataaa cacctggttt attgatatgt ccagatgccg aaattaattt
     8461 tgtgcctgtt gattttccta acccaatttt agcataagct tctccaccca tttttacaat
     8521 tggaacaact gccgccaatg tttcaacatt attcactaca gttggacagc catataaacc
     8581 agctacagct ggaaatggtg gtttaattct tggatttcct ctttttcctt ctaaagattc
     8641 taataatgct gtttcctctc cacaaatata agcaccacca cctggttgta catatatttc
     8701 caaattataa ttatagccta aaatattttt acctaacaaa ccagcttctc gagcttcttg
     8761 gatagctttt tctaatatct taataatata aaaaaattcg ccacgaacat agataaatgc
     8821 agtatttaca cctaatgcat agctagataa aatcatgcct tctatcaata aatgaggaat
     8881 tctttccatt aaatatctat ccttaaaagt tcctggttca gactcatcag cattacaaac
     8941 caaatagcgt ggaacacctt ctggtctagc caaaaaactc catttcatac ccgttggaaa
     9001 accagctcca cctctccctc ttaatccaga attttttact tctaattgta catcattcgg
     9061 ggataaacat tttaaagtat tctccaaagt ggcataacct ccgttttttc tataaacttc
     9121 taaggtatca atcccttcaa tatgctcgtt ttctaataat atttttctag acatgatgaa
     9181 tatgcttctt taacaatatc tacttctttt attttctttt ctaagttcct ctaaaataac
     9241 atcaattttt tcaaatgtga gattttcata atatttatct ccgatttgaa acatgggtgc
     9301 tgttccacaa gaccctaaac attctgctag tttaatttgg aacattccat ctgatgttat
     9361 ttcaccaact ttaacaccta atttgttttc taaataagcc acaacatctt cagcaccaca
     9421 catccaacaa gagcttgttt gacaaacttc tattaaatat tttccaactg gctctaaatt
     9481 aaacatggta taaaatgttg ccacttcata cacttctata ggtttaatat ttaaaatttc
     9541 agcaacataa tccatcgttt tcgggcttaa ccagccatca aactctgctt gtgctatatg
     9601 taaaattggt aataaggcag acttatgtct tccttcagga taccttttta taatttcttg
     9661 tactttttgt agtacttctg gtgtaaaact aattttttct gtagtcgttg acatatcttg
     9721 aagtatgtgc aaagataacg aattacacta aacttatgta ttatatttat taaaagtttt
     9781 acctttgagc ataatgaaaa aatatgtatt tatttttggt gcaggcagaa ctggaacaac
     9841 tttagctact cgaattattg agaaaagtga gaaagtcgcc ttatttaaac aagaatctca
     9901 tgcctatttg gctttttgga aatggggcaa tcaattgcaa attccatcaa aaattaaaac
     9961 acccaacgat tttgctgatt atgttttaga gcgatttcca gctataaatt tcggttggaa
    10021 aatggaaaat gaatatccta aattgttgaa attagtcgaa caaattaaaa atgaacaatt
    10081 ttttccaagg gataccaacg attttttaaa cttcctattt aagcaaattc caaatgaaaa
    10141 taataaagta gaatatattg gagagaaaac accagcacat atttattatt atcaagaaat
    10201 tctaaactat tttaaacctg caaaagttat tatcacaatt cgagacccaa gagcaactgc
    10261 ctattctgaa ttagttaaaa aaaatattaa agcattggga ttagcacctt ttaatatatt
    10321 gacatttatt gcacgattta atacggttta ttatttagtt gatgccatca aaaataaaat
    10381 tggtaaagaa aatgttttat tggttcgata cgaagatatt gttttgcaac ctgaggctac
    10441 catccaacaa atatgttcat ttatgcaaat tgaatttcaa gaagatatgt tacaaatggg
    10501 cgtttttaat agctcatttg gtgataaatt tcaaaaagat aaaaatttta atactgaaaa
    10561 tatagaccga tggcaaacag aattagattc aagttttatt gctcaaatcg aaaagaattg
    10621 taaaaatata atgcaaaagt acaattaccc tattagtaat ttaggaaatg gcagtgattt
    10681 aaagctcact gccaaactaa aactttttgt agcaaaatat tttgtacaat atttccccgc
    10741 tttatttcac tacttaaaca aacacgagaa gtacaggcta taaattattg atgactaaac
    10801 agttatgcta atcaatgatt agcattgcat caccataaga atagaaatta tatttctttt
    10861 ttatagcttg ttcataagcc tccattgtta aatcataacc agcaaaggca gcaaccataa
    10921 ttatcaaact tgattttggc aaatggaaat ttgtaatcat tgagtttgca atagaaaaat
    10981 cataaggtgg atgtacaaat aaatttgtcc aaccttccat tggttttaat aaatgttctg
    11041 ccgaaactgc tgactctatg gtacgcatag aagttgttcc aactgcacaa atattttttc
    11101 ctttttgttt tgctttattt acaatatcta ctgctttact ttctactttt acatactctg
    11161 catccatttt atgtttagat aagtcttcaa cttcaataga acggaaagta cctaatccaa
    11221 tatgtaaagt aacttcagca aaatcaacac ctttaatttc taatcttttg atgagttcat
    11281 tgctaaaatg caatccagca gttggtgctg ccacagcacc ttcttcttta gcaaataatg
    11341 tttgataata ttctttatcc aattcctctg gatctctttt aatatatttc ggtaatggtg
    11401 tattacccaa taagtctatt tttgctctaa actgttcatc tgttccatca aataaaaaac
    11461 ggattgttcg tcctcttgaa gtggtattgt ccactacctc tgccactaaa tcatcatcac
    11521 caaagtacaa tttattacct accctaattt ttctagctgg atcaactaat acgtcccaaa
    11581 gtcttaattc atgatttaat tctcttaata agaaaacctc gatttttgca cctgtttttt
    11641 ctttagtacc atacattctg gcaggaaaaa cctttgtatt gtttacaatc atcacatcgc
    11701 catcatcaaa ataattcaac acatctttga aaattttgtg ttcaattttt cctgattttc
    11761 gatgtacgac catcaattta cattcatcac ggtttttgtt gggataaaga gcgattttct
    11821 cttttggaag ttcaaattca aattgtgaaa gtttcataaa aatattattt tacggaataa
    11881 ttaatataca aagataaggt cataaaaagc ttattccaaa taatttaaaa atattcataa
    11941 atttataacc ttatgtttcg aattttagga gatacaattt taattagttt acagcttttt
    12001 tggtcaaata aaacacgtac ctttttatca ttatttggta ttagtattgg tattttcagt
    12061 attgtttcta tactcaccgc agttgatgca ctcagaggaa atatcaatga ttctataaaa
    12121 agtatgggcg ataatgtgct gtttataaac aaatgggctt ggacttttga cggtacttca
    12181 aattatcctt ggtggaaata cgcacaacgt ccagaagtga aatacaatat ttataaacat
    12241 ataagtagta aaacaaaatt aggtgcttat tccgcatttg aagtaaatcc aagatcaaca
    12301 ccagaaattt cttataaaag tagaaaaaca cgcaaagcaa tgatatctgc tgtaactgcc
    12361 gattatgcca aagtatttga tttagatata ttacacggtc gttttttatc atcaatggaa
    12421 gctaataatg gtgtaaacgt atgtattatt ggaaaagatg ttgctgataa actatttaaa
    12481 gatatagaaa atccagtcga ttatgaacta aaaattgatg gtcgaagtgt tagagtaatt
    12541 ggtgttttga ataaaaaagg agaaagtttg ttaggattca attttgacaa aagtattatt
    12601 ttgccttata cctattacaa caacaatttt agtataaatg aaaaaaatac ggattttacc
    12661 gttgaagtta gaacaaaaga tggtgttagc ttagatgaat tggctcaaga attaacaggc
    12721 ttgatgcgtg cagcgaggaa gcttagacca gtacaagctg atgactttac tatcaatcaa
    12781 ttgagtgtga ttgcaaaagg ttttgatagt ttattcagta ttttaggcat tataaaatgg
    12841 ataattggag gtttttcatt gttagtggga ggctttggta ttgccaatat tatgtttgtt
    12901 tcagtttctg aaagacaatc cattattggt ataaaaaaag cattgggagc caaaaattca
    12961 gatattttac tcgaattttt atttgaatct ataattcttt gtatgattgg aggtggaatt
    13021 ggtttattgt tggtatgggc aatcacagga ccagcatctg aggcaagtgg aatgcaattt
    13081 gtgttgagtg ctggcaatat tttagttgcc attagtgttt catttatatt aggtatttta
    13141 tcaggcataa ttcctgctta tatcgcagca aaaatggacc cagtcgaagc aattagaagt
    13201 aagtaatgtt atatttgttg agcaaaaaaa atcattaaaa gcaaattaag accgttgcaa
    13261 ggtaatcatt attcataacg ctcaagccaa attccttcaa tcttttttgt cctgaaaatg
    13321 gttactcata aataactaag aaagtgtgtg aaaatacgaa cctaaatata gtaatttaaa
    13381 aatttaagcc acaataaaac aaaagtttat tgtggcttaa tagttttagg ttttaattat
    13441 tcaatcccaa ttactttcat tagaagcggc atactcgcct cctccagcaa aaaataatgt
    13501 tattgctcca aataaaaaca aagctggcaa ttcaattccc caaccaccat tttcagaaaa
    13561 agtaaatatt tcatttcgat gtaccaaaaa gatggctgca cacatattaa tgataatcaa
    13621 cattgctgct atcttagttc taaatccaca aattaatagg actgttgcaa ataactctcc
    13681 cataaatact agataggcaa ctggagatat gtgtcctggc gaacccaaca tatcagaaat
    13741 ccaaccaact ccattgtata atttacttac accatgcact aataataagg ttccaattgt
    13801 tagtctaaga atcagtaacc ctgtacttgt atttgttttc ataatctagt atttaatagt
    13861 ttgaagttat atcaactttt actataaagc aaataaattt taatattttt ttaacaaaat
    13921 atgatttgaa taacaaaaac aattgacaaa agtcaataaa aattaattta taagtaaaaa
    13981 aaatagtatt tcaaatttga aatactattt ataaaattca aaagaaaatt aagctaaaat
    14041 attaattcaa cttattttag ttaatcaaat ttactagatg ttgaggcagc aaattttcct
    14101 gctccagaaa atactaaagc taatgcacct aacataaata aagctggcaa ttctattgtt
    14161 aatccacctt tgtcagagag tgtaaatata tccgctgaat gcactaaaaa tatcgccgtt
    14221 gccatattaa tagcgattat taatgcacca atttttgttc taaatccaaa aataatcatc
    14281 aaaggtgcta agacttctcc aataatagaa ccataagcaa taaaacctgg aataccttta
    14341 gcttctaaca tctgaaccat ccagtcaatg ccaaattgca atttagaaac accatgtaat
    14401 aataacaaaa ttccaattga taacctgagt atcaataatc ctaaattcaa atttgatttc
    14461 atattttttt tatttaaagt tatatattat ttttattatt gtcaaattta tttgtctgta
    14521 cttaatacca ttttaatttt taacctagct cctacttgca tcacatctac taaatcttgg
    14581 acagtcaatc cttttgctac tcttaaaacc acagtgttat caatgttatt tgccatagca
    14641 ctttgcaaag ctgcttctaa atctgtaaca ttcacttctg attgattgat ataatatttc
    14701 aaatcttcag ttaccgataa agtaacaggt tgtttggcta attgttcagt ttcttccgct
    14761 tttggcaata ataacttgat gatattttgg ttggccattg ttgaaataat caagaaaaac
    14821 aacatcaaaa aaaacataat atcattcatc gcagacatag cgtcttcgtc atgaaattct
    14881 tttcttcttc tgatttgcat aattattatt ttgtaggttt tttaattcct tttgtaaatt
    14941 caaaaactgc tttttgaagt tttaatgaat agccatcaat ttttgtaaga ataaaattat
    15001 aacccataaa tgcaataata ccaataataa gacctgtgcc tgatgaaatc atcttttcat
    15061 ataaaccgcc tgaaatagta ccaattgaaa tatcagaact taatgaaata ctataaaata
    15121 ttttaattac acctaaaata gtcccaacga aacccaaacg aggtgcaata ccagaaatca
    15181 aactgatata acccgtatgt cgttccatct ccatcaattc aatatttgat tgtgtttcta
    15241 aagctttttc aatgtggtca attggtttcc ctaattgcgt aatacctgct tgcaaaacat
    15301 ttcctaaagc agaattactc tgaatacaaa gtgcttcggc attttgaaca tttccagaat
    15361 acaatttatc caaaacatta ttcactaaat tcacatctat ttttgaatgt tgattaacat
    15421 acattattcg ttccacaata aaaccaatag caatcacgga aagtattagt aatggtatca
    15481 ttacgatacc acctttagtc agtaaaccaa ataaagagat actttctggt gctgtattag
    15541 caacagtttg aattgctgag gctgaatcta cacttgttgt aacttgaagt ataaaattta
    15601 gaatcgtcat aagtaaattt aatttctaca aatgtaacaa acggattaga ttattccacc
    15661 ttttgtttag aaaaattaac tatatatttg taaatacaat atttcaacta aaatcttaaa
    15721 atatgaacaa gtttttatgt tttataatga ttctactttt attttttcaa cataaggtta
    15781 tagcacaaaa attagacaac aatagacaag aatatttttt aaataaaaat atgcaaggct
    15841 cggaatatcg tttttctttt gtgcatatta cagatgttca catcggtgaa ggctttagtg
    15901 attatgggac ttatggcttt gcaaaggata caatgccaga tatggataat agcaaaccta
    15961 ctttatcttt aattcgtgct gtacaatgga taaatgaaaa ttatcaagct aaaaatatca
    16021 agtttgtaat agtttcaggc gatttgactg gtaaagctca acattctgaa tttgagaagt
    16081 ttaaatctat tatgagtaca ttagaaattc catatattcc aatgataggc aatcatgatg
    16141 tatggcaata tgttcgttat ggatttgaag aaccttaccc aactggcgat accgtaatca
    16201 acaccatttt taaagatgcc tttgagtctg ctaaacactt tttcgataat tggaatgatg
    16261 gaactcgatt gactgcgaca tttgatcccg aaagtggaca tgagcattat tttcaaaatt
    16321 ttagttttga atatgatggg tttaaatttt atgctttaga ttttaatcca agataccacg
    16381 tcaagaaaga agaacctgga attgggcctg aagctcaatt acatgatatt cctggcggaa
    16441 ctttccaatg gctaaaaaaa gaattgagtt tacaacccaa acataaagaa aacacaattt
    16501 tgctggctca tcatccgcta accgacaatt taatttacat cattgctggt tttgtttttg
    16561 aatatgacga atatgataaa cttacaaaaa tgcttattcc ttatagtaat aatttagctt
    16621 tatatttagc tggtcatgta catatttata atgattatca aattaggtct ttaaataagt
    16681 tgtataatat tatgccagct agagtcatgc cagcaagtaa agattttgat agtgcctatt
    16741 ttcaaattat tcatgtctat aagccacaag aagatttaac acctataaaa acaaataatt
    16801 taaacactaa tgatgttcta atttatccta atccaacaag tgatttatta aatattcagt
    16861 taaaggaaaa tattggagca attgatttta gtatcttaga tagtgaaggt aattatattc
    16921 taaaaacaaa ggctaataaa attgaccaat ataattatac actttccatt ggacaacttg
    16981 ctaaaggaaa ttatcaatta aaaattgata atgagaatta ttcaggaagt tttaaaattc
    17041 aaaaacaata attaagctac gacaactttt tttgctccac tcctacttaa tctaagattt
    17101 tggatttgtc ctgctgctga gaaaatggct ccaattatat ttaatccttg taataagata
    17161 ctttgtgaat gaaaacccaa atgtattatt gatgaaaaaa ttaaaatact gataccaata
    17221 ccaaataaat aagcgggtaa cttatcatga tgatggtatt tgtaaccatg cacaatagag
    17281 tttgttccta atagtaaagt tgaaaaaata acaactaatt ctaaccaatg gtatttttct
    17341 aaaaaaacag aagcaaatgg cattattgca ataaaaacag gcattgccaa acaatgtata
    17401 gcacataata aggaaaggta aatactaatt ttatttgcac tatgtttgtg gttttccata
    17461 attttatata cttttagata atcattacaa atgcaactca attgcaaata tacatgatat
    17521 atcttgattt ataatgttaa ataatgaaaa atattaaaga acgtctaaaa aaaagtggtt
    17581 tgcgagttac gccaattcgt gaggaagtac ttgaatattt tacagaccaa caaagagcct
    17641 tagcacacgc tgatatcgaa gccaattata aaaataaatt tgaccgagta acactttata
    17701 gaacgctgac aacttttatt gaaagtggca ttttgcataa agtttctgat aatagtggta
    17761 ttgcaaaatt tgcactttgc caacatcatg attgcaacca acacaatcat aaagataatc
    17821 atgtacattt taaatgtaat atttgtaata aaatagagtg tttacatgaa ttatctattc
    17881 cagattttaa attacctaaa aactatatta tgacaactgc caacttatta atagatggaa
    17941 tttgttcaaa ttgcaagtaa aacttatatt tataaaataa aaaaaacagc ctcatgaaat
    18001 cactttttaa aacagttata gtcatcgctt tagtttacgt tggcttatta ttttttggtc
    18061 ctaaacacat tcaaaatgga cttaaatatt ggttttccaa tgttgatgat tataaagttt
    18121 ttacaaataa tactatacaa aaatcatcgt caccaaaagc atggccaatt agttcagatt
    18181 ataataaaaa acaactttct gctgaagatg aaaaatatat gaccgataga aaaacttgtg
    18241 cttatttagt atttcaaaat ggacaattaa ttcatgaaaa atactatgat gactatgata
    18301 gttcaactat ttctggttca ttttctatgg caaaaactgt caatgcttta cttattggaa
    18361 aattgataga agaaggaaaa attggctcta ttaatgatga tattaaaaaa tatgtaccac
    18421 aattaacaca aattaaagaa ggtgaattga cgattaaaga tgttattaca atgagtgcag
    18481 atattgattg gaaagaatca tattggaata tattttctat gactgcagag tcttattatg
    18541 gtgaagattt agatcattta atgggaaaac ttaaagtccg aaaagaccaa caacaaggaa
    18601 aaatatggga ataccaaagt tgctgtacac agatattagg ttatattatt aaaaatgtat
    18661 ctaataaatc tatcagtgca tacactaaag aaattttgtg gcaaccttta ggtatggaaa
    18721 atgatgcttt gtggagtacc gataaaaatg gtggaattga aaagtcattt tgttgcttaa
    18781 atgctactgc tcgagatttt ggaaaattag gagcgttagt tttgcaaaaa ggaaaagtag
    18841 attctactca aattattcca gcacaatgga ttgcagacat gacaaaacca gcttcatacc
    18901 taaaaacagc tgatggcaga acttgcgatt ggtatggcta tcaaacttgg attattcagc
    18961 aaaacaatat gagtgtgcca tattttagag gagtacatgg gcaatttatt tttgttattc
    19021 ctgaaaaaaa tgcagtaatt gttcgtttag gtaaaaagat tgaaaagaaa aatattgatg
    19081 aacatgagca aaatgatgac atcaaagctt atttaagatt tggtttaagt ttattgaaat
    19141 aaaaaatatg tctttctttc taaataaaat gaataggatt acaacgattt atattctaat
    19201 cattttatac aaggtgcaat tcttttgaaa ctacattgaa taaatcttca aaaaaacacc
    19261 attaaatttt gtccattaga gcataacgtc taaaaaaaac attaaaaatg tccattactc
    19321 caaatattat tcaaaaaaat aggctgtccg tttcggacag cctatttttt attagcctaa
    19381 ttgttattct tagaattatt gtaccaatag ttgataagtt gctacaccat cttcattgtt
    19441 caaattcaat aaataaattc ctttagacag attttttatt
//
LOCUS       DAOQCW010000039        18450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1078_length_18450_cov_5.708888, whole genome shotgun sequence.
ACCESSION   DAOQCW010000039 DAOQCW010000000
VERSION     DAOQCW010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 18450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18450
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1078_length_18450_cov_5.708888"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="PLS16_05070"
     CDS             <1..715
                     /locus_tag="PLS16_05070"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HisS family protein"
                     /protein_id="HNJ60483.1"
                     /translation="VEWKEVAKELKDKNIHTTSIDKLHDLLTQKLNLAQLKSALAEIE
                     IAQEGIAEIEEIFYSLQILGIPEQYYTFDISLARGLDYYTGPIFETKLPKHPHIGSLN
                     GGGRYNGLIASFGGKDLPSVGTSLGLDRIFTAMQQLNLLTEVKTSTQVLVGNFGADTL
                     SASLKLVKKLRANGINTEFYPEATKMKKQFSYCDKKQIPFMAILGSEELAQNKITLKV
                     MKTGLQNLIDFNELLEKLA"
     gene            complement(755..1054)
                     /gene="raiA"
                     /locus_tag="PLS16_05075"
     CDS             complement(755..1054)
                     /gene="raiA"
                     /locus_tag="PLS16_05075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNJ60484.1"
                     /translation="MKVEIQSLHFNADASLVEFINKKMEKLDRFYDKIIGSDVVLSLE
                     QLNTQVKDKVVIIKTQIPGQILIAKETSKKFEEAVDLAVESIKKQMEKFKAKQPN"
     gene            complement(1081..1992)
                     /locus_tag="PLS16_05080"
     CDS             complement(1081..1992)
                     /locus_tag="PLS16_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNJ60485.1"
                     /translation="MVNEFLNYLKFEKRYSEKTILAYKIDIQQFVSFLFNEFSETTLE
                     KATYNQIRSWVVQLSLQETQASSLKRKVSALKSFFKFIIKKGIIDNNPADKITTPKLK
                     ERLPKYFEQKNINNLLNETNIFGENDSNATFKDTPEGKQDKLIIEMLYQTGMRRQELI
                     DLKWEAIDFSLKQLKITGKGNKQRLMPCSEKLLHLLEEHKATQNQRFEIKKDKNYIFL
                     TNNGQQLYPNYVYRLIKEKFRNVVAEKKSPHVLRHSFATHISNNGAKLSDIKELMGHA
                     SLASTQVYTHNTIEQLKEVYKKAHPKA"
     gene            complement(2051..2242)
                     /gene="rpsU"
                     /locus_tag="PLS16_05085"
     CDS             complement(2051..2242)
                     /gene="rpsU"
                     /locus_tag="PLS16_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNJ60486.1"
                     /translation="MLIVDAREQDSIDKALKVYKKKHDKAGVVKELRRRQAFTLSSVN
                     RRIQKQKAAYIEQLRKNEE"
     gene            complement(2313..3575)
                     /gene="metK"
                     /locus_tag="PLS16_05090"
     CDS             complement(2313..3575)
                     /gene="metK"
                     /locus_tag="PLS16_05090"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNJ60487.1"
                     /translation="MPYLFTSESVSEGHPDKVADQISDALIDNFLAYDPSSKVACETL
                     VTTGQVVLAGEVKSKAYLDVQEIAREVIRNIGYTKSEYMFEANSCGILSAIHEQSADI
                     NQGVERKNPEDQGAGDQGMMFGYATKETDTYMPLPLELSHLLLRELSAIRREGKVMKY
                     LRPDAKSQVTIEYNDNNQPIRIDAIVISTQHDDFGKEAAMLKQIKQDVIEILIPRVMK
                     KLPKRIQALFNNKIKYHINPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFSG
                     KDPSKVDRSAAYATRHIAKHLVAAGICDEALVQVAYAIGVAKPVGLYVNTYGTAKVNK
                     TDGEIAQIMGNLPEFDMRPYFIEKRFNLRTPIYSETAAYGHMGRESKVVEKTFNKGKD
                     NQLRIKVKLFPWEELNALATVKKAFGIK"
     gene            complement(3672..4748)
                     /gene="hisC"
                     /locus_tag="PLS16_05095"
     CDS             complement(3672..4748)
                     /gene="hisC"
                     /locus_tag="PLS16_05095"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNJ60488.1"
                     /translation="MKKINIDNIVNTHVKKMSAYSSARAEYKGKNAIFLDANENSIGS
                     VTKTIHNRYPDPLQIKVKEELAKLESVTIEKIFLGNGSDECIDVLIRCFCESGNDNIM
                     TFPPVFSMYEHAAHIQNIGVEEVLLLPETFQLDMDTILSKIKTNQQLKIIFICSPNNP
                     TGNLINKEDIETILNSFDGLVVVDEAYQDFANNKSWVSRINEFENLVVIKTFSKVWGM
                     ADARIGMLFANKSIIDYMNIIKMPYNVNQNSQNLALEALQNLAQKNNFVVKLNEGKKY
                     LLKELKQISYIQKVYPSDANFIVIKIDDANHLYHYLTNQHIIVRNRDKAPLMKGCLRI
                     SIGTQKENELLIDQLKNYKVPSHS"
     gene            complement(4756..6051)
                     /gene="hisD"
                     /locus_tag="PLS16_05100"
     CDS             complement(4756..6051)
                     /gene="hisD"
                     /locus_tag="PLS16_05100"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNJ60489.1"
                     /translation="MQTVKYPQKGQEYIEITKRATSNTSEITERVKTILQDVQQNGDA
                     ALKSFSEKFDKVQLETFQVSNNEIKEAIALVPEELKKAIQTAYQNIEKFHQQQVPTIN
                     VIETTIGVKCWRQNSAISNVGIYIPGGTAPLFSTVLMLAIPAKIAGCQNIVLCSPPNL
                     EGKIHEAILYTANLCGVTQIYKVGGAQAVASLAYGTESIPKVDKIFGPGNQYVTIAKQ
                     LVNVAGTAIDMPAGPSEVLVIGDKNANADFIAADLLAQSEHGTDSQSIFVTDSEDKLK
                     EVEIAILQQLELLPRKEITKKALENARLVLLNNLDECMDFSNFYAPEHLIIQTDNADE
                     LAQKVINAGSVFIGAYSPESAGDYASGTNHTLPTHGFATAYSGVSLDSFVKKITFQKL
                     SKEGLKNIGETVEIMASYEALDAHKNSVTIRLNAKTNQD"
     gene            complement(6062..6919)
                     /gene="hisG"
                     /locus_tag="PLS16_05105"
     CDS             complement(6062..6919)
                     /gene="hisG"
                     /locus_tag="PLS16_05105"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNJ60490.1"
                     /translation="MSILKLAIQKKGRLSEKSIELINECAISFPNSTGKLVAKCYDFP
                     MEILFLRDDDIPDYVQDGVADIGIVGENVAVESDKNVDDILKMGFGKCRLSLAVPRSF
                     QYNSIQDLQGKSIATSYPKILKNYLDKHNIQAEIHVISGSVEIAPSIGLADAICDIVS
                     TGSTLLSNGLKEVEQIFHSEAIIIGNKNLSEDKKVILDDFLFRLNAVRKAKKNKYILL
                     NAPNASLDKIIQILPGIKSPTVLPLAENGWSSVHSVITEKDFWQIIQQLKEAGAEGIL
                     VMPIEQMID"
     gene            7189..8601
                     /locus_tag="PLS16_05110"
     CDS             7189..8601
                     /locus_tag="PLS16_05110"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF005899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNJ60491.1"
                     /translation="MKTIHTYDIGILEDFDATETALKINKKEISREEAVACAIDRAMF
                     ANPSINAIVTPDFERALTNANKKYSNNGVFAGVPTFIKDLNDYAGLPTLMGSCGTPSI
                     PKQKNDPVVEQILKVTDSIVLGKSSSSEYGLLPCGETLQQGNTLNPWNLGHSTGGSSA
                     GSAALVAAGVVPFAHANDGGGSIRIPASCCGLVGLKPSRGRNVSSQSAMAPIDMAVDG
                     IVCRSVRDAANYFYGLEQFYTNPNLPKIGKVEHFDKKRLKIAVFTKSTTDVESHGDVV
                     DATMNAAKLCESLGHQIEFIDNPISYQFNKDFLMYWSFLSFGSILLEYQKYGLKFNPL
                     KATKFTKDLAKAYPFLSIHSVFSIKRLKQYNELYSQLFNKYDVLLSPTLSHPAPPIGH
                     YGPNVDTLEVIFRLNSYVNFTTLQNITGAPAISLPMGMSKDRLPIGIQFAANVGEEAK
                     LLSLAFEVERVNKFLKINEF"
     gene            8805..10928
                     /locus_tag="PLS16_05115"
     CDS             8805..10928
                     /locus_tag="PLS16_05115"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HNJ60492.1"
                     /translation="MSVTITENVSPETAKDIKELQIKIGAFIDGTMHGDKFKQFRLTR
                     GVYGQRQLGVHMFRIKIPMGRLNPAQLVRIAELSETYASSNLHFTTRQDIQLHYVKVT
                     DAPYVWAGLADCGLHAREACGNTVRNVTASSTAGIDPNELFDVSPYAYTAYSTFIRNP
                     ICQDMGRKMKIGFSASEEDSAFTFIHDFGFIPKIQDGKRGFSLVVGGGLGAQTFLAQK
                     AFDFIEEDQIIPFMEAALRVFDRYGERLRRHKARMKYLIKDLGMEKWMELVEEERTAT
                     KHKSFKIDLNLIPEAQPAPEKLVPDVIIEDKKRYQDWLNTNTFEQKQKGFYGVYLKVT
                     NGDIKPDTARTIAQLANDYASQDMRVTMNQGLLLKFIRPAALPYVYSVLDSLGLANPG
                     FGTIADITTCPGTDTCNLGVTNSMTLARILEDVIQKEYPELIYDKYLNIRMSGCMNAC
                     GQHMAANIGLHGSSIRVGDKVAPAMQVILGGGVDETGVGVIGDKVVKLPTKKMTDALR
                     TILNDYEANATDEEYFNTYYRRQGKNYFYTLLKPFADPTKFVDDDYFDHGHENYFTPE
                     VGVGECAGASYDMVGIIVNDAKIKQEEANEAMQNQLYAEAIYYAYTGFVVAAKALLLS
                     KDVECNTQIKILKDFDEHYVETAIVPVDGGFENLVLSINKNEPDADFAQQYVARYNSF
                     LEEVLVHRATITNAEKVVLESAYKA"
     gene            11039..11863
                     /locus_tag="PLS16_05120"
     CDS             11039..11863
                     /locus_tag="PLS16_05120"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNJ60493.1"
                     /translation="MGFIDSIQNLIGDKDKNKDFISKFPNKVGSYGYDAWGFNLKGIE
                     PFVEAGRFFYEKYFRVETYGLENIPKDGRVLIIANHSGQLPIDAILLGYALITNQYSP
                     RVPKGMYERFIPTIPFFSSLFSSWGGTLGDPENCIKMLENDEAVMVFPEGARGIAKPT
                     KLKYQLQRFGNGYIYMAVKTKTPIIPVGIVGCEEILINYGNIDFISKLIKAPSFPTLF
                     PFVFPSKVIMHFGEPIYFEGDIGREHIMEDMNKQVKSAVKELINEGLKKRKGIFSK"
     gene            complement(11895..13100)
                     /locus_tag="PLS16_05125"
     CDS             complement(11895..13100)
                     /locus_tag="PLS16_05125"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNJ60494.1"
                     /translation="MNTKKRSFESICIKDEMIENHHAHILPIYATSTFVYEDIEKAMD
                     YFKGKSDTHAYSRMSNPTPDFVAKKIARLEGFDETNSLETFGVLFGSGMAAISSAILA
                     CIEAGQKVLTTNNIYGTSNQFLASTLQTLGVGLVYSDLKDMQVFEQQLLADKSIKIIY
                     IETPTNPTLECYDLKSIASIAKKYQVYTIVDNTFSTPYLQRPLTLGFDIVVHSGTKFL
                     NGHGTGVSGVAVTTNEELYKKINQNKKVFGGICSPFEAYLLNNGIRTLSLRMKQHQAN
                     AQQLAEFLEQHPKITKVNYLGLKSHADHELAKQQMFGFGGMLSFEIDGNFDTAIHFMK
                     QIQFCTVTATLGTTDTLLTHSASTSHSNVPSEQRKAAGISDSLIRCSVGIEAIQDVID
                     DIQNALSKI"
     gene            13289..14341
                     /locus_tag="PLS16_05130"
     CDS             13289..14341
                     /locus_tag="PLS16_05130"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HNJ60495.1"
                     /translation="MLKNLYKLPRFFLFKMDAEAAHHFTLKMLKRTIKLPFAKSTLRA
                     LYQIEDKRLERNVFGLTFKNPVGLAAGLDKNAECIDAFAAMGFGFIEIGTVTPLAQLG
                     NDKPRLFRIIQDEAIINRMGFNNEGAERVRQNILSSELIKLKNRNVLIGGNIGKNKVT
                     PNENAVDDYIKCYHILYDVVDYFVVNISSPNTPNLRDLQEREPLEKLLSALQLENNKK
                     QTPKPILLKIAPDLSVTQLDEIIEVITATKLSGIIATNTTITRNGLSINQTAIEQIGA
                     GGLSGKPLTQKATEIIQYLSKKTEGKLPIIAVGGIMTAQDAIEKINAGASLVQLYSGF
                     IYKGIDLVKEINQKIL"
     gene            14400..15908
                     /locus_tag="PLS16_05135"
     CDS             14400..15908
                     /locus_tag="PLS16_05135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNJ60496.1"
                     /translation="MKIFSTQQMKELDRLTIQKKYSNSWELMENASTEIYKALLEQNI
                     SPKVPILFMCGCGNNGGYGLAVARMMAQKYRVQVFLLQSENYSSDNIRNQELLAATQT
                     KIHFFELHDTLQLPNNAIYIDALFGSGLNRTLEPAWNNIFKQIRAAQPKKIFAIDMPS
                     GLLDGFPMNENATCLSADLVFTLQSPKLSLLLPQNQEYAKDFKVLEIGLDKESIAETP
                     TNIYYIKEKFIKKLLKSPNVFAHKGTFGYVLIVGGNYGSIGAPILSTKAALKTGCGLV
                     TCAVPKCGYEIVQANFPEAMCIADEETYWISKIPQASNYNAIAIGMGLGKNEATKNAL
                     LSFLISNPTIPLVLDADALNILAETNDPFHYITKGSILTPHPKELERLIGTWANDYEK
                     IEKVRIIVKQYQIIILIKGAFSTIICPDDNIYFNSTGNWGMATGGSGDVLSGMIASLL
                     AQGYTPINATTLGVYLHGLSGDIASSISHPHSLIASDIIEHIGNAYKQLDNL"
     gene            complement(15903..16196)
                     /locus_tag="PLS16_05140"
     CDS             complement(15903..16196)
                     /locus_tag="PLS16_05140"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNJ60497.1"
                     /translation="MITRIVKLTMAEENIDTFKTYFDTVCNTIRNQPGCHKLQVWQDI
                     HHPQIFFTYSIWDSEDDLDKYRYSDFFAVFWKTVKPWFGAKAEVWSFDVLMDL"
     gene            complement(16193..16954)
                     /locus_tag="PLS16_05145"
     CDS             complement(16193..16954)
                     /locus_tag="PLS16_05145"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HNJ60498.1"
                     /translation="MAIITLLTDFSDKDYYVSMFKGKLLTTCPSVQIVDISNSIPSYD
                     IYTAALFLKSTYHHFPKNTIHIIRVFEKGIKHQQLLAMKYQDYYFIAPDNGILTLAFE
                     EKPNLIVRVDDLQLKTLDEYYCRVVKEIVFNNNIGSIGKATNNYVELKNPKPALNKDN
                     IEGQVIYIDSFGNVISNIRKEDFERYGDDINFRIYYRRKDYIDTFVDNYADVPRGMAM
                     AHFNSSGYLEIGINYGNAAQLLGLKIGTLIKIEII"
     gene            16996..18000
                     /locus_tag="PLS16_05150"
     CDS             16996..18000
                     /locus_tag="PLS16_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNJ60499.1"
                     /translation="MSELTIKLDTIDPIDFLGYNEGKLKIIKKIFNKLNIVARGNKII
                     AIGNQDDLVEFEQKIKLLVEYLERYGKITEEHIVDILNEENPYETVFPQFKDNVLVHG
                     NNGLLIKARTTNQQNMVDLSSENDILFAIGPAGTGKTYTAVALAVRALKNKTVKKIIL
                     TRPAVEAGENLGFLPGDLKDKVDPYLRPLYDALDDMIPADKLAYFMQNRIIEVAPLAY
                     MRGRTLDHAFMILDEAQNATQLQLKMFLTRIGPSAKCIITGDLSQIDLPRNQQSGLKS
                     TVEILKNIQGIGIIKLTEEDVVRHRLVKAIIKAFDKEKDRIDALKDKEKATQENSEKV
                     "
     gene            18010..>18450
                     /locus_tag="PLS16_05155"
     CDS             18010..>18450
                     /locus_tag="PLS16_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNJ60500.1"
                     /translation="MNSSNFQFPNQTNFYSGKVRDVYTINNDLMVMVVSDRISAFDVI
                     LPKPIPYKGQILNEIANHHLAQTIDILPNWVLEVPLPNVTIGRKCEPFKLEMVIRGYL
                     AGSAWRAYKNGIRTICGVTLPDGLVENQKLEQPIITPTTKASAGH"
BASE COUNT         6123 a         2718 c         3114 g         6495 t
ORIGIN      
        1 agtggagtgg aaggaggttg ccaaagaatt aaaggataaa aatattcata ccacaagtat
       61 tgataaatta catgatttat tgactcaaaa attaaattta gctcagttaa aatcagcatt
      121 agcagaaatt gaaatcgctc aagaaggcat tgctgaaatc gaagagattt tttattcctt
      181 gcaaatttta ggaatcccag aacaatatta tacattcgat atttctttag caagaggatt
      241 ggattattac acaggtccaa tttttgaaac aaaattacct aagcatccgc atattggttc
      301 attaaatggt ggtggaaggt acaatggatt aattgcttca ttcgggggga aagatttacc
      361 ttctgttgga acatctttag gtttagatag aatttttact gctatgcaac aattaaatct
      421 attgactgaa gtgaaaacat ctacacaggt tttggttgga aattttggtg cagatacttt
      481 atcagcatct ttgaaattag taaaaaaatt aagagccaat ggtataaata cagagtttta
      541 tcctgaagca acaaaaatga aaaagcaatt ttcttattgt gataaaaagc aaatcccatt
      601 tatggctatt ttaggaagtg aggagcttgc tcaaaataaa attactttaa aagtaatgaa
      661 aacaggtctg caaaatttga tagattttaa tgagttgttg gaaaagttag cataaaaaaa
      721 ggctgccctt ttcggacagc ctctcttttt ttaattaatt tggttgtttg gctttgaatt
      781 tttccatctg tttctttatc gattcaacgg ctaagtccac agcttcttca aattttttac
      841 ttgtttcttt tgctatcaaa atttgcccag gaatttgggt tttgataatg accaccttgt
      901 cttttacttg agtattgagt tgctctaaac tcaaaacgac atcagaccct atgattttgt
      961 cataaaaacg gtctagtttt tccattttct tgttgatgaa ttctacaaga cttgcgtcgg
     1021 cattaaaatg taaagattga atttctactt tcatcgtact ttatttagtg gtgaaaaaat
     1081 ttatgctttt ggatgagcct ttttataaac ttcttttaat tgctcaattg tgttgtgtgt
     1141 atatacttgt gtggaagcaa gacttgcatg tcccattaat tctttaatgt cagataattt
     1201 ggctccgttg ttgctaatat gcgtagcaaa tgagtgtctt aatacatgag ggcttttctt
     1261 ttctgcaacc acgtttctaa acttttcttt tattaatcta taaacgtagt ttggatataa
     1321 ttgttgtcca ttatttgtta aaaagatata gtttttatct tttttaatct caaagcgttg
     1381 gttttgtgtt gctttatgtt cttctaataa gtgaaggagt ttttcagaac aaggcattag
     1441 cctttgttta ttgccctttc cagttatttt aagttgcttt agactgaaat caatggcttc
     1501 ccattttaaa tcaatgagtt cttgtcgtct cataccagtt tggtagagca tttcaataat
     1561 taatttatct tgcttgcctt caggtgtgtc tttaaatgtg gcatttgaat cattttcgcc
     1621 aaatatattt gtttcattga gcaaattgtt aatattcttt tgctcaaaat attttggaag
     1681 tcgctctttt agttttggag ttgtaatttt atctgctgga ttattatcaa taataccctt
     1741 tttgataata aacttaaaga agctttttaa tgcagaaact tttcttttta agctgctagc
     1801 ttgtgtttct tgtaatgaaa gctgtactac ccaacttctg atttggttat aagtagcttt
     1861 ttctaaggtt gtttcagaaa attcattaaa taaaaaagaa acgaactgct gaatatcaat
     1921 cttatatgct aagattgttt tttcagaata acgtttctca aattttaaat aattcaaaaa
     1981 ttcgttcacc atacttcctc aatgtcattt agaacattaa ggtaatataa aaaaacgaaa
     2041 gttgcaaata ttattcttcg ttttttctta attgctcgat ataagcagct ttttgttttt
     2101 ggattcttct atttacagaa gataatgtaa atgcttgtct tcttctaagc tcttttacaa
     2161 cacctgcttt gtcgtgtttt ttcttgtaaa ctttaagtgc cttgtctatt gagtcttgct
     2221 ctcttgcatc aactattaac ataagttagt atttttttat tttttgagtt gcaaatatac
     2281 gtatttattc tgataaatca aacggataat ttttatttaa taccaaaagc tttttttaca
     2341 gtagctaatg catttaattc ttcccaaggg aataatttta cttttatcct taattgatta
     2401 tcttttcctt tattgaaagt tttttccacc acttttgatt ctctacccat gtgtccataa
     2461 gcagcggttt cactataaat aggtgttcta agattaaaac gtttttcaat gaaataagga
     2521 cgcatatcaa attcaggcaa atttcccata atttgagcaa tctcaccatc tgttttattt
     2581 actttggcag taccataagt atttacatac aagcctactg gtttagctac accaattgca
     2641 taagctactt gtactaatgc ttcatcacat atacctgcag ctactaaatg ttttgcaata
     2701 tgtctggttg cataagctgc acttctatca actttacttg ggtcttttcc agaaaatgca
     2761 ccaccaccgt gtgcaccttt gcctccgtaa gtatccacta taatctttct acctgtcaaa
     2821 ccagtatctc catgtggtcc accaattaca aatttaccag tcgggttgat atgatatttt
     2881 attttattat tgaataatgc ttgaattctt tttggtaatt ttttcattac ccttggaatt
     2941 aaaatttcaa ttacatcttg tttaatttgt ttaagcattg ccgcttcttt gccgaaatca
     3001 tcatgttgag tagaaattac aattgcatca attcttatag gctgattatt gtcattatat
     3061 tctatggtaa cttgcgattt ggcatcagga cgtaaatatt tcattacttt accttctctt
     3121 ctaatggcag aaagttctct taataataaa tgagaaagtt ctaaaggtaa aggcatataa
     3181 gtatcagttt cttttgtagc ataaccaaac atcatacctt ggtcaccagc accttgatct
     3241 tcaggatttt ttctctctac accttgatta atatcagcag attgctcgtg tatagcagaa
     3301 agaataccac aagaatttgc ctcaaacata tattcagatt tagtatatcc aatatttctt
     3361 attacttctc tagcaatttc ttgtacatct aaatatgctt tagattttac ttctccagct
     3421 aaaactactt gtccagttgt tactaaagtc tcgcaagcaa cttttgaaga tgggtcatag
     3481 gctagaaagt tatcaataag tgcatctgaa atttgatctg caactttatc tgggtgtcct
     3541 tctgaaacag attctgacgt gaataaataa ggcatatttg ttcttttatt tgttagtgtt
     3601 tattttttaa tgctcaaata taattaaaag tcaataattt tcttaataat cacttcttat
     3661 tttaagcaat tttatgaatg ggaaggtact ttataatttt ttaattgatc aattaaaagt
     3721 tcattttctt tttgtgtgcc gatgcttata cgcaaacaac ctttcattaa tggagctttg
     3781 tctctatttc taacgatgat atgttgattg gtcaaatagt gatataaatg attcgcatcg
     3841 tctattttta ttacaataaa attcgcatca gatggatata ccttttggat ataagagatt
     3901 tgttttagtt ccttcaataa gtattttttg ccttcattta attttacaac gaaattattc
     3961 ttttgtgcta aattttgtaa ggcttcaaga gctaagtttt gagaattttg gtttacatta
     4021 taaggcatct taataatgtt catataatca ataatagatt tatttgcaaa caacattcca
     4081 attcgagcat ctgccattcc ccaaactttt gagaacgttt taataacaac taagttctca
     4141 aattcattga ttctacttac ccaagattta ttgttagcga aatcttgata ggcttcatct
     4201 acaactacta aaccatcaaa tgaatttaat atcgtttcaa tatcttcttt gttaattaaa
     4261 ttcccagttg gattgttagg cgaacaaata aaaatgattt ttaattgctg atttgttttt
     4321 attttggata aaatggtatc catatccaat tggaaagttt cagggagtag taaaacttct
     4381 tcaactccta tattttgaat atgtgcagca tgttcgtaca ttgaaaatac tggaggaaat
     4441 gtcattatat tgtcattgcc tgattcacaa aaacagcgaa taagaacgtc aatacattca
     4501 tcacttccat tacccaaaaa tattttttca attgttacag attctaattt tgctagttcc
     4561 tcttttactt ttatttgtaa aggatcagga tatctattat gaattgtttt ggtaacagaa
     4621 ccaatactat tctcgtttgc atctaagaat attgcatttt ttcctttata ttcagcacgt
     4681 gccgatgagt aggcactcat ttttttgacg tgtgtattga caatattgtc tatgtttatt
     4741 tttttcatta cgattttagt cttggttagt ttttgcattt agtcgaatag taactgaatt
     4801 tttatgagca tctaatgctt cataacttgc cattatttca acagtttctc ctatattttt
     4861 caatccttct ttggataatt tttggaaagt aatttttttt acaaaactat ctaaacttac
     4921 tccactataa gcagttgcaa atccatgcgt aggtaaagtg tgattggtgc cacttgcata
     4981 atcaccagca ctttcaggag aataggctcc aataaaaaca gaaccagcat tgattacctt
     5041 ttgagctaat tcatctgcat tatctgtttg aataattaaa tgttctggag cataaaaatt
     5101 agaaaagtcc atacactcat ctaagttatt caacaatacc aaccttgcat tttctaatgc
     5161 ttttttagtg atttcttttc taggtaatag ttctaattgt tgtaaaatag caatttctac
     5221 ttcttttaat ttatcctcgc tatcagttac aaaaatagac tggctatctg taccatgttc
     5281 tgattgtgct aataaatcgg cagcaataaa atctgcattt gcatttttat ctccgataac
     5341 caatacttca cttggaccag ctggcatatc aatcgctgta ccagcaacat ttactaattg
     5401 cttagcgatg gttacatatt ggtttcctgg accaaaaatc ttatctactt ttggaatact
     5461 ttccgttcca taagctaatg aagctactgc ttgagctcct cctactttat aaatttgggt
     5521 tacaccacag agattagccg tgtaaagaat tgcttcatgg atttttcctt ctaggtttgg
     5581 aggtgaacaa agaacaatat tttgacatcc agcaatctta gcgggaattg ccaacatcaa
     5641 aacagtcgag aataaaggtg cagttccgcc aggaatataa atacctacat ttgaaattgc
     5701 actattttgt cgccaacatt ttacgccaat agttgtctct ataacattaa tagtaggaac
     5761 ctgttgttga tgaaattttt caatattttg ataggcagtt tgaatcgctt tttttaattc
     5821 ttcaggaact aaagctattg cctctttaat ttcattattg ctaacttgaa aggtttctaa
     5881 ttgtactttg tcaaattttt cagaaaagct tttcaatgca gcatctccat tctgttgcac
     5941 atcttgtaag atggttttta ctcgctcggt aatttctgaa gtattagatg ttgctctctt
     6001 agtgatttcg atatattctt gtcctttttg aggatatttt acagtttgca taatctctgt
     6061 tttagtcaat catttgttcg ataggcatta ctaaaatacc ctcggcgcct gcttctttta
     6121 attgttgaat gatttgccaa aaatcttttt ctgtaattac ggaatgtaca gaactccaac
     6181 cattttctgc cagtggtaaa actgttggac ttttaatgcc tggtaaaatt tgaataattt
     6241 tatctaagct tgcatttggt gcatttaata aaatatattt attcttttta gcttttcgta
     6301 cggcatttaa tctgaataag aaatcatcta agattacttt tttatcttct gataagtttt
     6361 tattcccaat gatgatggct tctgaatgaa atatttgttc tacctctttc aatccattac
     6421 ttaataatgt tgaacctgtg gaaacgatat cacaaatagc atctgctaat ccaatgcttg
     6481 gagcaatttc tactgagcca gaaatgacat gtatttcagc ttgaatattg tgtttgtcta
     6541 aatagttttt tagaattttt gggtaagatg ttgcaataga ttttccttgt aagtcttgaa
     6601 tagagttgta ttgaaaactg cgaggaacag ctaatgatag gcgacatttt ccgaatccca
     6661 tttttaaaat atcatcgaca tttttatcag attcaacggc aacattttct ccaacaattc
     6721 caatatcagc aacaccatct tgtacataat ctggaatatc atcatcacgt aagaataaaa
     6781 tttccatagg aaaatcatag cattttgcaa ctagttttcc tgtactattt ggaaatgaaa
     6841 tagcacattc gtttatcaat tcgattgatt tttcgcttaa acgtcctttt ttttgtatcg
     6901 ctaattttaa tattgacata gtataaaatt actttagtaa agtgaataaa tataaataaa
     6961 tagaaagatt gatgaaaata taaaatacac tcctacttgg agtgatggaa ttgagaatgt
     7021 aaaataaatg cgattgcttt catctaaaaa caaagataag gatatttaga taacgtaatt
     7081 ttaaataata gttatatgta cttatagtgt atttttaaag attttgtgtt ttataaatcc
     7141 cattttagaa agaaaattta gttattttta gaaaatataa atatatagat gaaaacaatc
     7201 cacacgtacg atattggtat cttagaagat tttgatgcga cagaaacagc tttaaagatt
     7261 aataaaaaag aaatctcaag agaggaagct gtggcttgtg ccatagatag agcaatgttt
     7321 gcaaatcctt ctattaatgc aattgtaacg cctgattttg agcgagcatt aaccaatgca
     7381 aataagaaat atagtaacaa tggtgtgttt gctggtgttc caacttttat taaagatttg
     7441 aatgattacg ctggtttacc taccttgatg ggtagttgtg gaacgccttc tattcctaag
     7501 caaaagaacg atccagttgt ggagcaaatt ctaaaagtaa cagatagtat agttttagga
     7561 aaaagtagct cttcagaata tggattgttg ccttgcggag aaaccttaca acaaggaaat
     7621 acgctaaatc cgtggaattt aggacattca acaggtggtt catctgcggg ttctgctgct
     7681 ttggtggctg caggtgttgt gccttttgca catgcaaatg atggaggagg ttctattcgt
     7741 attcctgctt cttgttgtgg tttggtaggt ttaaaaccat caagaggtcg gaatgtctct
     7801 tcacaatcag caatggctcc aatagatatg gcagttgatg gtatcgtttg tagaagtgtg
     7861 cgagatgcgg caaattattt ttatggttta gaacaatttt atacgaatcc taatttgcct
     7921 aaaattggaa aagttgaaca ttttgataaa aagcgattga agatagcggt ttttacgaaa
     7981 tctacaacag atgtagaaag tcatggagat gtagttgatg cgacgatgaa tgctgctaaa
     8041 ctttgtgaaa gcttaggtca tcaaattgaa tttattgata atccaattag ctatcagttc
     8101 aataaagact ttttaatgta ttggtcattt ctgtcttttg gaagtatttt gttagagtat
     8161 caaaaatatg gcttaaaatt taatccatta aaagctacaa agtttacaaa agatttagca
     8221 aaagcctatc cattcttgtc cattcattct gtttttagta taaaaagatt gaagcaatat
     8281 aatgaactat atagtcaatt gtttaacaaa tatgatgttt tattaagccc aacactttcg
     8341 catcctgctc caccaattgg gcattatgga ccaaatgtag ataccttaga agtaattttt
     8401 aggttgaata gttatgtgaa ttttactact ttacaaaata taactggagc tccagcaatt
     8461 tctttgccga tgggtatgag taaagataga ttaccaattg gaattcaatt tgcagctaat
     8521 gttggagaag aagcaaagtt attgtctttg gcttttgaag tggaacgtgt taataaattt
     8581 ttaaaaatta atgaatttta atagtcttat gtaacaaaag ttacataatc atagatttct
     8641 ttttatattt gtttgaatga aattgttttt tgaaaaatca aattgttgta gttgttgtct
     8701 aacctaatgg tatgataggg caaaaattat tattaaaata tatgtaacct atcttattga
     8761 agataggttt ttttataaat caaattaatt aaaataaaca aaaaatgagt gtaactatca
     8821 cagaaaatgt atcacccgaa acggcaaaag atatcaaaga attacaaatt aaaattggtg
     8881 cattcattga tggtacaatg catggagata aatttaagca atttagatta actcgtggtg
     8941 tttatggaca gcgtcagttg ggtgtacaca tgtttaggat taaaattccg atgggcagat
     9001 tgaacccagc tcagttagtt cgtattgcag aattgtcaga aacttatgca tcttctaatt
     9061 tgcattttac aaccagacaa gatatacagt tgcattacgt aaaagtaact gatgcacctt
     9121 atgtttgggc aggtttagca gattgtggtt tacatgctag agaagcttgt ggaaatacag
     9181 taagaaatgt tacagcatct tcaaccgcag gtatcgaccc aaatgaattg tttgatgttt
     9241 ctccttatgc ttatacagca tatagtacat ttattcgcaa cccaatttgt caagacatgg
     9301 gtagaaaaat gaagattggt ttctctgctt cagaagagga tagtgctttc acatttattc
     9361 atgattttgg atttattcct aaaattcaag atgggaaacg aggttttagt ttagttgttg
     9421 gtggaggatt aggagctcag acgtttttgg ctcaaaaagc ttttgatttt atagaagaag
     9481 accaaattat tccatttatg gaggctgctt taagagtatt tgatagatat ggagaaagat
     9541 tacgacgtca taaagctaga atgaaatatt tgataaaaga tttaggaatg gaaaagtgga
     9601 tggaattagt agaagaggag cgtacagcta ccaaacataa gtcttttaag attgatttga
     9661 atttaattcc tgaagctcaa ccagcacctg aaaaacttgt tcctgatgta attatcgaag
     9721 ataaaaagag ataccaagat tggttaaata caaatacatt tgaacaaaaa caaaaaggct
     9781 tttatggtgt atatctaaaa gtaacaaatg gcgatataaa acctgacaca gcaaggacta
     9841 ttgctcaatt ggccaatgat tatgcatcac aagatatgcg tgtaaccatg aatcaaggtt
     9901 tgttgttgaa gtttatacgc ccagcggctt tgccttatgt atattcagtt ttggatagtt
     9961 taggattggc caatcctgga ttcggaacaa ttgcggatat tactacttgt ccaggtacag
    10021 atacttgtaa tttgggtgta acgaatagta tgacattagc tagaatatta gaagatgtta
    10081 ttcaaaaaga atatcctgaa ttaatttatg acaaatactt aaatatcaga atgagtggct
    10141 gtatgaatgc ttgcggacaa cacatggctg caaatattgg tttacatggt agctctatca
    10201 gagtaggaga taaagttgca ccagcaatgc aagtgatatt aggtggaggt gttgatgaaa
    10261 ctggcgttgg tgtaattgga gataaggtgg tgaaactgcc aactaaaaaa atgaccgatg
    10321 ctctacgtac tattcttaat gattatgaag caaatgctac tgatgaggaa tattttaata
    10381 catattatag aagacaagga aagaattatt tttatacgtt attgaagcca tttgcagacc
    10441 caactaagtt tgtagatgat gattattttg accacggaca tgaaaattat tttacacctg
    10501 aagttggtgt gggtgagtgt gctggtgcat cttatgatat ggtaggcata attgtaaatg
    10561 atgctaaaat aaaacaagaa gaagcgaatg aagctatgca aaatcaactt tatgcagaag
    10621 ccatttatta tgcttatact ggttttgtcg tagcggcaaa agcactttta ttgagtaaag
    10681 atgtggaatg taatacacaa attaaaatac tgaaagattt tgatgagcat tatgttgaaa
    10741 cagcaattgt tccagttgat ggtggatttg aaaatctagt attaagtatt aataaaaatg
    10801 aaccagatgc cgattttgca cagcaatatg tcgctcgtta taatagtttt cttgaggaag
    10861 tattggtaca cagagcaaca ataacgaatg ctgagaaagt ggtattagaa agtgcttata
    10921 aagcatagtt atagatatta acttataaga taagtgatat acaacaaatg ataaaaatat
    10981 tatcttttgt ttattcggaa ttgctttcaa ttatcaattt tattatgtag gtttgagtat
    11041 gggtttcata gatagcattc aaaatctaat aggcgataaa gacaagaata aagactttat
    11101 ttctaaattt ccaaataaag ttggaagtta tggttatgat gcttggggtt tcaatcttaa
    11161 aggaattgag ccttttgtag aagcgggtcg atttttctat gaaaaatatt tccgtgtaga
    11221 aacttatggc ttagaaaata ttcctaaaga tggacgtgta ttgattattg ccaatcatag
    11281 cggacagctg cctatagatg ctattttatt agggtatgca ttaattacta accaatattc
    11341 gcctagagtt cccaaaggta tgtatgaacg atttatacct actattcctt tttttagttc
    11401 attgttttct tcttggggtg gtactttagg ggatccagaa aactgtatca agatgttgga
    11461 aaatgatgaa gctgttatgg tttttccaga aggagcaaga ggtattgcaa agccaacaaa
    11521 attgaagtat cagctacaaa gatttggtaa tggttatatt tatatggcgg taaaaacaaa
    11581 aacaccaatt ataccagttg ggattgtggg ttgtgaggaa attttaatca actatggaaa
    11641 tatagatttt ataagtaagt tgataaaagc accctctttt cctacattat ttccgtttgt
    11701 ttttccatca aaggtcatta tgcatttcgg tgagccaatt tattttgaag gcgatattgg
    11761 aagagagcat attatggaag atatgaataa gcaagtaaaa agtgccgtaa aagaacttat
    11821 caatgaggga ttgaaaaaac ggaaaggtat tttttctaaa taattattct tattctatta
    11881 taaatttcta ccgattatat ttttgacaga gcattctgaa tatcatcaat aacatcttgt
    11941 atggcttcaa taccaacaga acatctaatt aaactatcag aaatgcctgc tgcttttcgc
    12001 tgttcagatg gtacatttga atgacttgta cttgcagagt gagtaagtaa agtatcagtg
    12061 gtgccaagtg ttgcagttac ggtacaaaat tgaatctgct tcataaaatg aattgcagta
    12121 tcgaaatttc catcaatttc aaaactcaac ataccaccaa acccaaacat ttgttgcttt
    12181 gctaattcgt ggtctgcatg actttttaat cctaaataat ttactttagt aatttttgga
    12241 tgttgctcta agaattcagc taattgttga gcattggctt gatgctgttt cattcttaaa
    12301 cttaaagtac ggatgccatt atttaataga taagcttcaa atggagaaca aatgccacca
    12361 aaaacttttt tattttgatt gatttttttg tataattctt catttgtagt aactgccaca
    12421 cctgaaactc cggttccgtg tccgtttaaa aatttagttc cagaatgtac aacaatatca
    12481 aagcctaatg ttaatggacg ttgtaaatat ggtgttgaaa aagtattatc tacaatagta
    12541 taaacttgat atttttttgc aatagatgca atagatttta agtcgtaaca ttctaaagtt
    12601 ggattggtgg gcgtttcaat ataaatgatt ttgatacttt tgtctgcgag taattgttgc
    12661 tcaaaaactt gcatatcttt caaatctgaa tatactaatc caacgcctaa tgtttgtaag
    12721 gttgatgcta aaaactgatt gctagttcca taaatattgt ttgtcgtcag cactttttgt
    12781 cctgcttcaa tacaagccaa aattgctgag ctgattgcag ccattcctga accaaataaa
    12841 actccaaaag tttctaggct atttgtttca tcaaaacctt ctaatcgagc aatctttttc
    12901 gctacaaaat ctggtgtagg gttgctcatt ctcgaatagg catgcgtatc agattttcct
    12961 ttaaaataat ccattgcttt ttcaatatct tcatatacaa aagttgaagt cgcataaatg
    13021 ggcaatatgt gtgcatgatg gttttcaatc atttcatctt tgatgcaaat agattcaaaa
    13081 cttctttttt ttgtgttcat aagaatgtat tttaaataaa tgaataggtg tgtttgattg
    13141 tagaattagc agaaacatac atcaaaatta caaattttgg cgactaagcg aaaataaatt
    13201 aacatcaatc aacaaacaag ttaatatagt tttgatttcc agataaatga ataataatat
    13261 ctttgtgcaa tacaaactta aacggataat gcttaaaaat ttatataaac ttccacgctt
    13321 tttcttgttt aaaatggatg ctgaagctgc acatcatttt actttaaaga tgcttaaaag
    13381 aactataaaa ttgccttttg ctaaaagtac tttaagggct ttatatcaga ttgaagataa
    13441 aagattagaa cgaaatgtat ttggtctgac ctttaaaaat ccggttggat tggctgcagg
    13501 attagataag aatgcggagt gtattgatgc ttttgctgca atgggttttg gttttattga
    13561 gattggaact gttacgccat tagctcaact aggaaatgac aaacctagat tgtttagaat
    13621 tatccaagat gaggcaatta ttaatagaat gggttttaac aatgaaggtg ccgaaagagt
    13681 aagacaaaat attctatcgt cagaattaat aaaacttaaa aataggaatg ttttgatagg
    13741 tggaaatatt ggcaaaaata aagtaacacc taatgaaaat gcggtagatg attatatcaa
    13801 atgttatcat attttatatg atgtagtaga ttattttgta gtgaatatta gttcaccaaa
    13861 tacacccaat ttgagagatt tgcaagaaag agaacctttg gaaaagttac tttctgcact
    13921 tcaattagaa aataataaga aacaaacacc taagcccata ttgttaaaaa tcgctcctga
    13981 tttaagtgtt actcaattag acgaaattat tgaagtgata acagcaacaa aattaagtgg
    14041 aattatagca accaatacaa caattactcg taatggatta agcattaatc aaacagcaat
    14101 tgaacaaatt ggagctggtg gattgagtgg taaaccgcta actcaaaaag ccacagaaat
    14161 cattcaatat ctttccaaga aaacagaagg taaactacca attattgcag tcggtggcat
    14221 tatgactgct caagatgcaa tagaaaaaat taatgctggt gcaagtttag ttcaattata
    14281 ttctggattt atttataaag gcatagactt agtcaaagaa atcaatcaaa aaattttata
    14341 aacaaaatca tagttatttc gtttataaaa gggtattctg atttatcttt aaaatataaa
    14401 tgaagatttt tagtacacaa caaatgaaag aattggatag attaactatt cagaaaaaat
    14461 attccaattc ttgggaatta atggagaatg cttcaacaga aatatataaa gctttattag
    14521 agcaaaatat ttcacccaaa gttccaatat tatttatgtg tggatgtgga aataatggtg
    14581 gctatggttt agctgttgct aggatgatgg ctcagaaata tagagttcaa gtgtttttat
    14641 tgcaatctga aaattatagc tctgataata ttagaaatca agagttattg gcagccactc
    14701 aaactaaaat tcattttttt gaactgcatg atactttgca actacccaat aatgcgattt
    14761 atatagatgc tctttttggc tcaggattga atcgtacttt agaacctgct tggaataata
    14821 tttttaaaca aatcagagcc gcacaaccta aaaaaatatt tgccatagat atgccaagtg
    14881 gcttattaga tggttttcca atgaatgaaa atgcaacttg tttgtcagca gatttagtat
    14941 ttacattgca atctccaaag ttgtccttat tgcttccaca aaatcaggaa tatgctaaag
    15001 attttaaagt tttagaaatt ggactagata aagaaagtat tgctgaaacg cctacaaata
    15061 tttattatat taaagagaag ttcattaaaa aattgttgaa atcacctaat gtatttgcac
    15121 ataaaggcac atttggttat gtattaatag ttggaggaaa ttatggcagt attggagctc
    15181 caattctatc aacaaaagca gctttgaaaa ctggatgtgg cttagttact tgtgcagtcc
    15241 caaaatgtgg ttatgaaatt gtacaagcta attttcctga agcgatgtgt attgcagatg
    15301 aagaaactta ttggataagc aaaatacctc aagcatcaaa ttataatgca attgcaatag
    15361 gaatgggatt gggaaaaaat gaagccacca aaaatgcatt gttgagtttt ttaatttcta
    15421 atccaacaat tcctttagtg ttagatgctg atgctttgaa tatcttagct gaaacgaatg
    15481 acccatttca ttatattact aaaggtagta ttttaacacc acatccaaag gagctagaac
    15541 gactaattgg aacttgggca aatgattatg aaaaaataga gaaagtaaga ataattgtaa
    15601 aacaatatca aattataata ttgataaaag gagctttttc tactataatt tgccctgacg
    15661 ataatattta tttcaatagc acaggaaatt ggggaatggc aacaggtggt tctggtgatg
    15721 tattgtctgg tatgatagca tcacttttgg ctcaaggtta tacgccaatt aatgctacaa
    15781 ctctaggtgt ttatttacat ggactttcag gtgatattgc tagttcaatt tcccatccgc
    15841 atagtttgat tgcttcagat attattgagc atattggaaa tgcatataag caattggata
    15901 atttataaat ccattaagac atcaaaactc cacacctcag cctttgctcc gaaccaaggt
    15961 tttactgttt tccagaagac tgcaaagaaa tcggaatatc tatatttatc caaatcgtct
    16021 tcgctatccc aaatgctata cgtgaaaaat atttgtggat gatgaatgtc ttgccaaact
    16081 tgtaatttgt ggcaacctgg ttgatttctg atggtattac atacagtatc aaaataagtt
    16141 ttgaaagtat ctatattttc ttcagccatc gttaatttta cgattcttgt aatcatatta
    16201 tttcaatttt tataagtgtt ccaatcttta agcccaataa ttgtgcagca tttccataat
    16261 ttataccaat ttctaaataa ccactagagt taaaatgagc cattgccatt cctcttggca
    16321 catcagcata attatctaca aacgtatcga tataatcctt tcttctataa tatatcctaa
    16381 agtttatatc gtcgccatat ctttcgaaat cctcttttct gatattgctt attacgtttc
    16441 caaaactatc aatataaatt acctgacctt cgatattatc tttatttaga gcaggctttg
    16501 gattttttag ctccacataa ttattcgtag ctttgcctat acttccaata ttgttattga
    16561 aaacaatttc tttaaccact ctacaataat attcatctag tgtctttaat tgtaaatcgt
    16621 ccactctaac aattaaattt ggtttttcct caaatgctaa agtcaagata ccattatctg
    16681 gagcaataaa ataataatct tgatatttca tcgctaataa ttgttggtgt tttatacctt
    16741 tctcaaaaac tcgaataata tgtattgtat tttttggaaa atgatgataa gtacttttta
    16801 aaaataaagc agcagtataa atatcataag aaggaattga atttgaaata tctacaattt
    16861 gaacagatgg acaggttgta aggagcttac ctttaaacat actaacataa taatccttgt
    16921 cgctaaagtc tgttaataaa gtaattatcg ccattctgca aaaaaagtta atttcaagta
    16981 ctaaaatagg aataattgag cgaactaaca attaaattag atacaataga tccaatagat
    17041 tttttggggt ataatgaagg taaactaaaa atcattaaaa agatatttaa caagttaaat
    17101 attgtagcac gtggtaataa aattattgct attggcaatc aagatgattt agtagaattt
    17161 gagcaaaaaa taaaattatt ggttgaatat ttagaacgtt atggtaaaat tacagaagag
    17221 catatcgttg atattctgaa tgaagaaaat ccgtacgaaa ctgtttttcc tcaattcaaa
    17281 gataatgtgt tggtacatgg aaataatggc ttactaatca aagctagaac taccaatcaa
    17341 caaaatatgg ttgatttatc atctgagaat gatattttat ttgcaattgg accagcagga
    17401 accggaaaaa cttataccgc agtggctttg gctgttcgtg ccttgaaaaa taaaacagta
    17461 aagaaaatta ttttgacaag accagctgtt gaagcaggtg aaaatttggg tttcttgcca
    17521 ggtgatttga aagataaagt agatccttat ttacgtccat tgtatgatgc attagatgat
    17581 atgattccgg ctgataagtt agcttatttt atgcaaaata gaattattga agtggcacct
    17641 ttggcatata tgcgaggtcg tactttagat catgctttta tgattttaga tgaagcacaa
    17701 aatgcgacgc aattgcaact taaaatgttt ttaacaagga ttgggccatc agcaaaatgt
    17761 attattactg gagatttatc gcaaatagac ttgccaagaa accaacagtc tggattgaaa
    17821 agcacagtag agattttaaa aaatattcaa ggtattggta ttataaaact tactgaggaa
    17881 gatgttgtta gacatcgatt agtaaaagca attattaaag catttgataa agaaaaagat
    17941 agaattgatg ctttaaaaga taaagaaaaa gcaacacaag aaaattcaga aaaagtataa
    18001 cttagaagaa tgaattcatc taattttcaa tttccaaatc aaactaattt ttatagcgga
    18061 aaagtacgtg atgtttatac gattaataat gatttgatgg tcatggtggt ttctgatagg
    18121 atttctgcat ttgatgttat cttaccgaaa ccaattcctt ataaaggaca aattttgaat
    18181 gaaattgcca atcatcattt ggcacaaacc attgatatat tgccgaattg ggtattggaa
    18241 gttccattac caaatgttac tataggcaga aaatgtgagc cttttaaatt ggagatggtg
    18301 attcgtggtt atttagcagg ttcagcttgg cgagcatata aaaatggcat cagaacaatt
    18361 tgtggtgtta cacttccaga tggattggtt gaaaaccaaa aactagagca acctattatc
    18421 acaccaacga caaaagcttc agctggtcat
//
LOCUS       DAOQCW010000040        18284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1090_length_18284_cov_6.539031, whole genome shotgun sequence.
ACCESSION   DAOQCW010000040 DAOQCW010000000
VERSION     DAOQCW010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 18284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18284
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1090_length_18284_cov_6.539031"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            294..821
                     /locus_tag="PLS16_05160"
     CDS             294..821
                     /locus_tag="PLS16_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNJ60501.1"
                     /translation="MSLIIPCNNISPKFGNNCYLAPNCTIVGDVEMGNDCSIWFNAVL
                     RGDVNSIRIGNKVNIQDNATIHCTYQKTKTIIADNVSIGHNAIVHGCTIEENVLVGMG
                     AIIMDNCYIEKNALIAAGAVVLEGTRVEEGSIYAGVPAKKVKQLEAATFKDQNERIAN
                     NYLKYSSWFKTDKNE"
     gene            814..1884
                     /locus_tag="PLS16_05165"
     CDS             814..1884
                     /locus_tag="PLS16_05165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNJ60502.1"
                     /translation="MNKKSKILFLSPYPFGKAPSQRLKYEQYYDAFQNAGFDITTSSF
                     ISLSFWKIIYQEGHYFGKIFYTVLGYFRRCFDLLRIRKFDVVYVHLWVTPLGFPIFEY
                     LVCLFAKKVIYDIDDMIYLNNNSNRWIDKIKGRNKPIVLMKKANHIVVCSPFLADFVR
                     QYNTNITTILATYDTEKSLPLKTHIDKEPIIIGWTGTHSTLKYLSIIAPVLEKISKIR
                     NIKFLVICNAIYKNSNIEVENIAWSAEHEYEDLRMIDIGLYPLEKEEWVLGKSGNKAL
                     AYMNLGIPCIATNYGTNQVVIENGKNGFLADDASDWEKYLLQLIDDIELRKRIGTKAR
                     QSVEAKYSVQKNTQTYLDLIVQ"
     gene            complement(1901..2545)
                     /locus_tag="PLS16_05170"
     CDS             complement(1901..2545)
                     /locus_tag="PLS16_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNJ60503.1"
                     /translation="MTSIKSFCFNFFQENTYVIWHNTTKNCWIIDPGCMNTQEQQQLF
                     NFIETNGMKPIRLLNTHCHLDHIYGNKAVADKYQLELGIHQEDVKILDAASLSARMFG
                     APIPEKLEPSYFLDEQSKIDLDGVEFKILFTPGHSPGSICFYNAIDKYCIVGDVLFSR
                     SIGRTDLPYGDYDTLIESIKTKLYTLEDSTEIYNGHGVPTSIAQEKQNNPFVRG"
     gene            2598..5882
                     /locus_tag="PLS16_05175"
     CDS             2598..5882
                     /locus_tag="PLS16_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60504.1"
                     /translation="MKQFLVYIVLFFVVVVAKSQTTQVEFGQNKVQYKKFNWQYLETD
                     NYDLYYYNQSENFAKYIHTIADNTIKNLIDKLGYSNRIKFDILVFNSAEDFAQTNIGL
                     NNETYNIGGTTKVNENKIFIYYNGNYQTFQNDLKKALVDVLTRQMMKGGSFGQKLTNF
                     LFLRLPVWYNIGVVNYIAYGWNTQLDDALRQNFIHYKNISFNQLIKENPSLAGQSFWY
                     MLAEEYSENSISNILYLTRINHNINTGFRLAINKDLSNLSDQWLQFYSERYQADIKNR
                     KVFPSEMLLQQKGKIASVQTNAANNKMVYSLIKKNKQKIKVTELPKKNSQVILKNKFN
                     SNNNFAWSKDNRLLAISYYKNGKIYIKIKDYSTKKTITNKIENLDKIYALDFSYNNDR
                     LVLSAQKNGQTDLFQYSISSNSSSQITNDIFDQLNPKVSKWNGLEKILFSSNIPSKSI
                     GDFSTYNFDALDLFVSDINGKNIERLTTTPNANEVPIGNFNADYFTFMSDENGINNQY
                     IGNLPTSTISDTIDQTKKTIFAFNYNPITNFEKNIEAINVTNTSNIYLVNDVINKRKT
                     KSKIYSENRLSTTAITDGITPTNFIASTKLGKHSNNNFNTQTTIQPNMFNDNQTNLDS
                     IYLGNFPYTFQTKFKNRITTHKSDSIPNETKPSVNASESASENNTVEQINQVEKFAIL
                     NTLPVVSPYKSKFATDYFTTQLDNSLMNTYQSVQQNQGRYEFPSVGILLKVGVSDIMD
                     NHRLSGAIRMPFTFDGSEIALRYDALKHRVDGHLSFYSRFNKVGFDVFDGTGASIGNY
                     IARTSTYIADAGLSFPFNDKNALKVSGGYRNEQFTPTYTDANAFALKKQTENWINGKL
                     EFVHDGTEEIQFNIPKGLKFKTFFEYYKNINSKGLNTYNVGFDLRYYLKIHKNLIWAN
                     RVAFASSFGTQKILYFLGGVDTWLNQKYDNNIPIDGTTNFGLQAPVNNMRGLPQNIRN
                     GNNYLLWNSELRWPLFAFFAKKALQSDFIRDFQLIGFFDAGMAYSEANPFDKENAYRK
                     EIINPGNPMEITLKTYRNPFVYGFGGGIRSNIMGYFIRIDAGWGHNGKTLSTKPIWHI
                     SLSKDF"
     gene            complement(5889..8837)
                     /locus_tag="PLS16_05180"
     CDS             complement(5889..8837)
                     /locus_tag="PLS16_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNJ60505.1"
                     /translation="MSTIVNPYQIDPSILDGLATQLEGDLFVDEKMRILYATDGSVYK
                     EYPLAVAYPKNESDIQKLIHFSNQYKVALIPRAAGTSLGGQVVGNGIVVDISKHLNKV
                     IEVSTEGSWVRVEPGVIRDELNYYLKDYKLYFAPETSTANRAMIAGMVGNNSCGANSI
                     VYGSTRNHVLELKGFLSNGDAISIKALDDNEIDNKIQQKNREGEIYRFLFDNLSNPDT
                     QAQIKENFPKESIHRRNTGYAVDVLLEQTPFEPTGRAFNLAELICGSEGTLMFITEIK
                     LNLVPTPPKYKIVVAAHFQTLEESLKATVFAMKFAPSACELMDKVILDCTKANIEHRK
                     NRFFVKGDPEAILCVEFSSDDTAYAQKMAEQFVLLLEKENLGYQYPLIEGNDINKVWS
                     LRKAGLGLLANIPGDPKAVAVVEDTAVHIEDLPAYIMEFEQMMQKYGQKSVYYAHAGA
                     GELHLRPILDLKLKKDRILFRQIGEETAHLVKKYDGSLSGEHGDGRVRGEFIPLMVGE
                     ANYQLLRDLKYTFDPENLLNPNKIVDTPPMDTSFRYEEDQKTNQFDTVLDFSEDQGIL
                     RAAERCNGCGECRKLPLSGGTMCPSYMATKNEKDTTRARANILRDFLTNSDKENKFDH
                     QEIYDVMDNCLSCKGCTSECPSNVNIPALKAEFLHQYYKEHGVPLRTKAIANINSLNK
                     LGSKFAKLSNYFMTNKFFSSQMKKRLGIAEKRSLPYIYAESLEQWFMKIKDKQKVKYR
                     NGKVYLFIDEFTNFNDVPIGQKAVQLLNKLGYEVDYIKHKESGRAHLSKGLLDKAKEL
                     ANYNVSIFTSWINNDTPLIGIEPSTILTFRDEYLRLVEEPLKDKAKNLAKNCFMIEEF
                     IYQEMEKGNITNKSFTKEPKVIKLQGHCHQKSLSSADYSAWIMGLPENYVVDVIPSGC
                     CGMAGSFGYEKEHYDLSMQIGELVMFPAIRAAEKAIIASSGTSCRHQIKDGTARIALH
                     PIEVLFDALV"
     gene            complement(8919..9230)
                     /locus_tag="PLS16_05185"
     CDS             complement(8919..9230)
                     /locus_tag="PLS16_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HNJ60506.1"
                     /translation="MHIMENNNQINIELPEDIAEGEYSNLAIITHSNQEFVMDFIRVA
                     PGIPKAKVKSRIILTPQHAKRLMKALQDNVKKFEQLNGEIKDNEAVALPMNFGTPTTQ
                     A"
     gene            complement(9287..13606)
                     /gene="rpoC"
                     /locus_tag="PLS16_05190"
     CDS             complement(9287..13606)
                     /gene="rpoC"
                     /locus_tag="PLS16_05190"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNJ60507.1"
                     /translation="MPLKKDSKVKAEFNKITLKLASPDVILSRSCGEVKKPETINYRT
                     YKPEMDGLFCERIFGPSKDYECYCGKYKRIRYKGITCDRCGVEVTEKKVRRERMGHIK
                     LVVPIVHIWYFKSLPNKIGYVLGLSSKNLESIIYYERYAVIQAGILEDGIMINGEKKT
                     YKYLDLLSEEEYLDVMDALPKENLLLPEEDPNKFIAKMGGEGVRDLLANIKLTALAAE
                     LRHSASNETSQQRKSEALKRLSVVEAFREANEHTENRPEWMVVHYLPVIPPELRPLVP
                     LDGGRFASSDLNDLYRRVIIRNNRLKRLIEIKAPEVILRNEKRMLQEAVDSLFDNSRK
                     SNAVKADGGRALKSLSDGLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELKMHECGLPK
                     DMAAELFKPFIIRKLLERGVVKTVKSAKKLVDKKESVVWDILENVMKGHPVLLNRAPT
                     LHRLSIQAFQPKLIEGKAIQLHPLVTTAFNADFDGDQMAVHVPLGNAAILEAQLLVLS
                     SHNILNPQNGSPMTIPSQDMVLGLYYLTKGKKTTETEKVLGEGRIFYSAEEAIIALNE
                     KKADIHAWVKVKTKVLENGKLVDKIIETTLGRIIFNQNVPASCGFVNVLLTKKNLKDV
                     IGNVLTTTDIPTTVNFLDNIKEMGYRQAFKGGLSFNIDDLIIPDLKQNLLNEALLEVE
                     DVWQNFNTGLITNNERYNQIIDIWTRVDTRITEGLMKEISSTKQGFNSVYMMLDSGAR
                     GSKQQIKQLAGMRGLMARPRKSGSAGGDIIENPILSNFKDGLSVLEYFISTHGARKGL
                     ADTALKTADAGYLTRRLVDVAQDVIINAEDCGTLRGLNVAALKEGDDIIESLSDRIVG
                     RTALLDVVHPVSGEIIASEGELINEATAKQIEESGIEEVEIRSVLTCELDRGVCSKCY
                     GRNLTNNQKTQMGDAVGIIAAQSIGEPGTQLTLRTFHAGGAAASVAADSSLLAKFDGK
                     LEFDDVKSVKQEAEDGSVKQVIIGRTGEINIVDEESGRVLITNNIPYGSILAVKSGQK
                     VKKGDVICSWDPYNNVVVSEFKGTVKFEDIIENVTYREEIDEQKGHIEWVTIESRDKK
                     KAPAIKVVDDKGKELASYSMPVGSHIIVKDNVAVNTGTILVKIPRILSKVRDITGGLP
                     RVQELFEARNPSNPAKVSEIDGQVTFSKAIKRGNREITVTSKDGVRKKYLVSLTKHIL
                     VQDGDFVKAGMPLSDGSITPADILNIKGPGALQAYLVNEIQEVYRLQGVKINDKHIEV
                     IVRQMMSNLLIEDAGDTKFLEGDTVNRFEFVKVNDDIFDKKVITEAGDSQELYAGQIV
                     TMRQIREENSNLKKNDLKTLEYRDALPATGSPLLQGITKSSLGVDSWISAASFQETTK
                     VLNRAAINASIDYMQGLKENVIVGHRISAGTGVKAFQEINVANKKEHDKLVKKAFLEE
                     DED"
     gene            complement(13787..15634)
                     /gene="glmS"
                     /locus_tag="PLS16_05195"
     CDS             complement(13787..15634)
                     /gene="glmS"
                     /locus_tag="PLS16_05195"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNJ60508.1"
                     /translation="MCGIVGYIGKKKAFPIIVNGLKRLEYRGYDSSGVAILDEEHNLN
                     VYKKKGKVVEMEAFALANNIEGNIGIGHTRWATHGEANDVNAHPHISTDGDIAIIHNG
                     IIENYNALKKALEQRGYQFRSQTDTEVVAHLIDDIQKNVHVDLAEAVRLALNEVVGAY
                     AIVVISKKEPDKLVVAKMSSPMVIGFGDGELYVASDGSPLIEYTRRVSYIEDGHMAVL
                     HLNGEIEIKNLKDNAVQSPYIEELSISLEAIEKGGFEHFMMKEIHEQPQAILNSIRGR
                     MSLEQMKISLGGINKFESSFKNAERIIIVACGTSWHSGLIGEYLIEELARVNVEVEYA
                     SEFRYRNPVLSEKDIVIAISQSGETADTKAALELAKQKGAFIYGICNVVGSSIARMTD
                     AGSYTHAGPEIGVASTKAFTTQVSILVLLALQLAELRGTISSSKLNEFLNELDILPSK
                     IDKILESDTYIKEIADAFQDSSNFLYLGRGFNFPVALEGALKLKEISYIHAEGYPAAE
                     MKHGPIALIDKNMPVVVLAPNCQHYEKVISNVEEVKTRKGRIIAVVTEGDTEVEALAE
                     YVIEIPNTLEALSPILSVVPLQLLAYHIAVMRGCNVDQPRNLAKSVTVE"
     gene            complement(15646..17013)
                     /locus_tag="PLS16_05200"
     CDS             complement(15646..17013)
                     /locus_tag="PLS16_05200"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HNJ60509.1"
                     /translation="MKQYFLILLAFTAIFSCKKADNIGGDILPNSDYLNLELSDTFLL
                     TSKTIAESPVRTDKLIFNYIGVLQSAEYGKNHAKLMFELANPTIALADSLAPYTIDSA
                     VLFMKYNIVYGDTNSTQNIVVKTLNDNILENQKYYSDNAIFFGTNEVGRANNIALKPS
                     QKFFTYTGDTLGSVSIIRVPMEASYAQTYVDKIGSSDSALIKYANFDNYFRGLEVAFE
                     NNLGNAMALVDLTSDKTRFTIFLKDKNGVRKEMNMYPRLTSVVKESTTYLQSSAINVY
                     GNTYNSSVNNALSSTELTDSINFIIGQAGLMNKITIPDFSNLINQQAAVNKAELYVTQ
                     TNSNADTSSNVSIIYALYKDNSGNFKISGIGAKDSVYVNGNNEKVARYNINISTIVKE
                     MWLGRLTTRDIYLSNHNINQNTDAELNDISYLGGYAPTKLTIGGTNSSDIYQKTRLKI
                     FYSKQ"
     gene            complement(17019..17828)
                     /locus_tag="PLS16_05205"
     CDS             complement(17019..17828)
                     /locus_tag="PLS16_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNJ60510.1"
                     /translation="MEQKRLLFVAQEMDPYLALTEMASLVKRLAPMATDNGWEVRVLM
                     PRFGVINERRNRLHEVVRLSGINIIIDDLDYPLIIKVASLPGTRIQVYFLDNEEFFKR
                     KLVFRDEEENFLEDNGERMIFFCKSLIETVRKFGWTPDIIHCHGWFTSLIPMYLRTHY
                     AKDPIFTNSKIIYSLYEQDFQEELNNGFLKKAKISEQIKDKDIQLLEGLNNVCLNKLG
                     IKFADAISIGSEELSDVDTFKKLAAKKPWLAYQEDIDIQQKYLDFYNQIIG"
     gene            17917..>18284
                     /locus_tag="PLS16_05210"
     CDS             17917..>18284
                     /locus_tag="PLS16_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNJ60511.1"
                     /translation="MKVFSSIIEIRKFLDEQSESQLIIGFVPTMGALHQGHLSLIEQS
                     KKSCDITICSIFVNPTQFNDKKDFEKYPIQIDADLEMLIAAGCDVVFIPSVDEMYPEG
                     TTQDKLLVDLGFLGKTLEAE"
BASE COUNT         6037 a         3195 c         2566 g         6486 t
ORIGIN      
        1 tttctgtttt aattggtgtc gcaatcggcg aaaaaataat tggcttacat attttaggat
       61 tactagtgat tttctctggt atttatagcg tcattaaagg ctaaattctc ttgagttttg
      121 ttttcaatca ttgcatgttt tttcttagaa ccaaaagttg gacagccaca ttttttagat
      181 ttataagctg cacaagcact aaaactcaaa gaaaaaattg aaataaaaat taaaagcaaa
      241 aatctttttt gtattttgaa accattcttc attgttgtat aaaaataata aaaatgtcat
      301 taataattcc atgtaataat atttctccaa aatttggcaa taattgttac ctcgctccaa
      361 attgtaccat tgtaggcgat gttgagatgg gaaacgattg tagtatttgg tttaatgctg
      421 ttttgcgtgg agatgtaaat tccattagaa ttggcaataa agtgaatatt caagataatg
      481 caacaataca ttgtacttac caaaaaacaa aaacaattat tgcagataat gtatcgattg
      541 gacacaacgc tattgtgcac ggttgcacca tcgaagaaaa tgttttagta ggcatgggtg
      601 caattattat ggacaattgc tatattgaaa aaaatgcttt gattgcagct ggagcagttg
      661 ttttagaagg aactcgtgtt gaagagggaa gtatttatgc tggtgtccct gcaaaaaaag
      721 taaaacaatt agaagctgct acttttaaag atcaaaatga aagaattgca aataattatt
      781 taaaatattc aagttggttt aagacagata agaatgaata aaaagtcaaa aatattattt
      841 ttatctcctt atccttttgg caaagctccg agtcaaagac tgaaatatga gcaatattat
      901 gatgctttcc aaaatgcagg ttttgatata acaacaagtt cttttattag cttatctttt
      961 tggaaaatca tttatcaaga aggacattat tttggaaaaa tcttttatac tgtcttaggt
     1021 tattttcgca gatgttttga tttattacgc attaggaaat ttgatgtggt gtatgttcat
     1081 ctttgggtaa cacccttagg tttcccaatt tttgaatatt tagtttgttt atttgccaaa
     1141 aaagtcattt atgatattga tgacatgatt tatttgaaca ataattctaa tagatggatt
     1201 gacaaaataa aaggaagaaa taagccgatt gtattgatga agaaagcaaa tcatattgtg
     1261 gtttgttctc cattcttagc agatttcgtt cgtcaatata atacaaatat cacaacgatt
     1321 ttagctactt acgacactga aaaatctttg ccattgaaaa ctcatattga caaagagcct
     1381 atcattattg gttggacagg tacgcactct actttaaaat atttaagtat tatagctcca
     1441 gttttagaaa aaatttctaa aataagaaat ataaaattct tagtcatttg caatgcaata
     1501 tataaaaact caaatattga agtagagaat attgcttggt ctgctgaaca cgaatatgaa
     1561 gatttgagaa tgatagatat tggtttgtat ccattagaaa aagaggaatg ggttttgggc
     1621 aagagtggaa ataaagcctt agcatatatg aatttaggaa taccttgtat agccacaaat
     1681 tatggaacaa atcaagttgt aattgagaat ggcaaaaatg gatttttagc tgatgatgct
     1741 tcagattggg aaaaatattt attgcaactg attgatgata ttgaattaag aaaacgcatt
     1801 ggaacaaaag caagacaaag tgtagaggct aaatattctg ttcaaaaaaa tacacaaact
     1861 tatttggatt taattgtaca ataggttgat ttatatacaa ttatcctcga acaaaaggat
     1921 tgttttgttt ttcttgggca atacttgttg gaacaccatg tccattataa atttcagtac
     1981 tatcttccaa agtatataat tttgtcttga tactttctat caaggtatca taatcgccat
     2041 aaggtaaatc tgttctgcct atacttctac taaacaacac atctccaaca atacaatatt
     2101 tgtctatcgc attataaaaa caaatgcttc ctggcgaatg tcctggagta aataagattt
     2161 taaattctac accatctaaa tctatttttg attgctcatc taaaaaatag ctaggctcta
     2221 atttttcagg gattggagcg ccaaacattc ttgccgaaag tgatgcggca tctaatattt
     2281 tcacatcttc ttgatgaata cctaattcta attggtattt atcggcaacc gctttgtttc
     2341 cataaatatg atcgagatga cagtgtgtat ttaataaacg aataggcttc atgccattgg
     2401 tttcaataaa attaaacaat tgttgctgtt cctgagtatt catacaacct gggtcgataa
     2461 tccaacaatt tttagtcgta ttgtgccaaa taacataagt attttcctga aaaaaattaa
     2521 aacaaaaact cttaatactt gtcattatct ttgtagttgt cataaacttt atttattttg
     2581 aattaaatta tattttattg aaacagtttt tagtatatat tgtcctattt tttgttgttg
     2641 tagttgcaaa atcacaaacc acacaagtag aatttggaca aaataaggtg caatataaaa
     2701 aatttaattg gcagtatttg gaaactgata attatgattt gtattattac aatcaatctg
     2761 aaaatttcgc taaatatatc catacaattg ctgataatac aattaaaaat ttgatagata
     2821 aattagggta ttccaataga ataaaatttg atattttagt ttttaattca gctgaggact
     2881 ttgcacaaac caatatcggc ttaaataatg agacctataa tattggagga actacaaaag
     2941 tcaatgaaaa taaaatattt atatactata acggcaacta tcaaaccttt caaaatgatt
     3001 taaaaaaggc attggtagat gtccttacaa gacaaatgat gaaaggtgga agctttggac
     3061 aaaagttaac taatttttta tttttacgat tacctgtttg gtataatatt ggcgtggtca
     3121 attatatcgc ttatggttgg aatactcaat tagatgatgc tttaaggcaa aattttattc
     3181 attataaaaa catcagtttt aatcaattaa taaaagaaaa tccaagtttg gcagggcaat
     3241 cattttggta tatgttggca gaagaatatt cagaaaattc gataagtaat atattatacc
     3301 ttacaagaat caatcataat atcaatactg gatttagatt ggcgatcaat aaagatttaa
     3361 gtaatttatc cgaccaatgg ctacaatttt attcagaaag atatcaagct gatataaaaa
     3421 acagaaaagt atttcctagt gaaatgcttt tacaacaaaa aggcaaaatt gcatctgttc
     3481 aaactaacgc agccaataat aaaatggttt atagtttaat taaaaaaaat aagcaaaaaa
     3541 taaaagtaac agaacttcca aagaaaaata gtcaagtaat tctaaaaaat aaattcaatt
     3601 caaataataa ttttgcttgg agtaaggaca atcgcctttt agcgataagc tactataaaa
     3661 atggaaaaat ctatataaaa attaaagatt attcaacaaa aaaaacgata acaaacaaaa
     3721 ttgaaaactt agataaaatc tatgctttag atttctccta caataatgat aggttggtat
     3781 tgtctgcaca aaaaaatgga caaactgatt tatttcaata tagcatttct agtaattcaa
     3841 gtagtcaaat cacaaacgat atttttgacc aattaaatcc aaaagttagc aaatggaatg
     3901 gattagaaaa aattcttttt agttccaata ttccttccaa atctattgga gatttttcaa
     3961 cctataattt tgacgcatta gatttgtttg tttccgatat caacggaaaa aatatagaac
     4021 gtttaaccac aacgccaaat gcgaatgaag ttccaatcgg caattttaat gctgattatt
     4081 ttacttttat gagtgatgaa aatggtatta ataatcaata tatcggaaat ttaccaacta
     4141 gtacaataag cgatactatt gaccaaacga agaaaactat ttttgcgttt aattataacc
     4201 caattactaa ttttgaaaaa aatattgaag cgattaatgt taccaatact tccaatattt
     4261 atttagtaaa tgatgtaata aataaaagaa aaacaaaaag caaaatctat tctgaaaata
     4321 ggctatcaac aacagcaata acagatggga tcacaccaac taattttata gcttcaacaa
     4381 aactaggaaa gcatagcaac aataatttta atacacaaac gaccattcag cctaatatgt
     4441 ttaatgataa ccaaaccaat ttagatagta tttatcttgg aaattttcct tatacatttc
     4501 aaacaaaatt taagaataga attacaaccc acaaatcaga cagtattcca aatgaaacaa
     4561 aaccatcagt aaatgcaagt gaatctgctt cagaaaacaa taccgttgaa caaattaatc
     4621 aagttgagaa atttgcaatt ttaaacacat tgcctgtggt ttcgccatac aaatcaaaat
     4681 ttgcaacaga ttattttaca acacaattag ataattcatt gatgaatact tatcaaagtg
     4741 tacaacaaaa tcaaggtcgt tatgagttcc caagtgttgg tattttatta aaagttggtg
     4801 ttagtgatat tatggacaat catcgtttaa gtggtgcaat cagaatgcct tttacttttg
     4861 atggttcaga aattgcatta agatatgacg cattgaaaca tagagttgat gggcatcttt
     4921 cattttatag tcgttttaat aaagttggat tcgatgtgtt tgacgggaca ggtgcaagta
     4981 taggaaatta tattgccaga acttcaactt atattgcaga tgctggacta agttttcctt
     5041 ttaatgataa aaatgcatta aaagtttctg gtggttaccg aaatgaacaa tttacgccaa
     5101 cctacacaga cgccaatgct tttgcattaa aaaagcaaac tgaaaattgg ataaatggca
     5161 aattagaatt tgtacacgat ggtacagaag agattcaatt taatattcca aaaggtctaa
     5221 aattcaaaac tttttttgaa tactacaaga acattaatag caaaggttta aatacttaca
     5281 atgttggttt tgatttgcgt tattatttaa aaattcataa aaatctaatt tgggcaaacc
     5341 gtgtagcttt tgcttcttcc tttggaacac aaaaaatatt atatttttta ggtggcgtag
     5401 atacttggtt gaatcaaaaa tatgataaca atattccaat tgatggcaca actaattttg
     5461 ggttacaagc acctgttaat aatatgcgtg gcttgccaca aaatattaga aatggcaata
     5521 attatctatt atggaattcc gaattaagat ggcctctatt tgcatttttt gctaaaaaag
     5581 cattacaatc tgactttatc agagactttc aattgattgg attttttgat gcaggaatgg
     5641 cttatagcga agccaatcca tttgacaagg aaaatgctta tagaaaagaa attatcaatc
     5701 cagggaaccc aatggaaata acattaaaaa cctacagaaa tccatttgta tatggatttg
     5761 gaggtggcat tcgctcaaat attatgggtt attttataag aattgatgca ggttggggac
     5821 ataatggcaa aacactttct accaaaccaa tatggcatat ttctttaagt aaggattttt
     5881 aagagaattt atactaaagc atcaaataac acttctattg gatgtaaagc aattcttgct
     5941 gtaccatctt ttatttgatg gcgacagctc gttcctgatg atgcaataat tgctttttca
     6001 gcagctcgaa ttgctggaaa catcactaat tctcctattt gcatagataa atcataatgt
     6061 tctttttcat aaccaaaact tcctgccatt ccacagcaac cgctcggaat tacatctaca
     6121 acataatttt caggaagtcc cattatccaa gctgaatagt ccgcagagga taatgatttt
     6181 tgatgacaat gcccttgtaa tttgataacc tttggctctt ttgtaaaaga tttattagta
     6241 atattgcctt tttccatttc ttgatagata aattcttcta tcataaagca atttttagct
     6301 aaattttttg ctttatcttt taatggctcc tctaccaacc ttaaatattc atctctgaaa
     6361 gttagaatcg ttgaaggctc aatgcctata agtggtgtat cattatttat ccaacttgta
     6421 aaaatagaaa cattatagtt tgccaattct ttagccttat ctaaaagtcc tttagataaa
     6481 tgagctcttc cgctttcttt atgttttata taatcaacct cgtagcccaa tttattcaat
     6541 aactgtacgg ctttctgacc aatcggcaca tcattgaaat tggtaaattc atctataaac
     6601 aaataaactt ttccgtttct atattttacc ttttgcttat ccttgatttt cataaaccat
     6661 tgctccaatg attccgcata gatgtaaggt aaacttcttt tctctgcaat gcctaaacgc
     6721 ttcttcatct gagaggagaa gaatttattg gtcataaaat agttgcttaa tttagcaaac
     6781 ttagagccta atttatttag agaattaata tttgctatgg cttttgttct caaaggaaca
     6841 ccgtgttctt tataatattg atgtaaaaac tcagctttta aagctggaat atttacgtta
     6901 gatggacatt ctgaagtaca ccctttacaa gataaacagt tgtccattac atcataaatt
     6961 tcttgatggt caaacttgtt ttctttatct gaattggtta gaaaatctct taaaatatta
     7021 gctctcgctc gagtggtatc tttttcattt ttggtagcca tataagaagg acacattgtt
     7081 cctccactta atggtaattt tctacattcg ccacaaccat tacaacgctc tgctgctcgc
     7141 aaaattcctt ggtcttcact aaaatctaaa acggtatcaa attgatttgt tttttggtct
     7201 tcttcatatc taaatgaagt atccattggt ggcgtatcca caattttatt aggatttaat
     7261 aaattttctg ggtcgaaagt atattttaaa tctcgtaata attgatagtt tgcttctccc
     7321 accatcaaag gaataaattc gcctcgcacc cgaccatcac catgttctcc actcaatgag
     7381 ccatcgtatt tttttaccaa atgggcggtt tcttctccta tttgtctaaa taaaattcta
     7441 tcttttttga gttttaaatc taaaatagga cgcaaatgca attctcctgc tcctgcatgt
     7501 gcataataca ctgatttttg tccgtatttt tgcatcattt gctcaaactc cataatataa
     7561 gctggcaaat cttcaatatg caccgctgta tcttcaacca cagcaacagc tttcgggtct
     7621 ccaggaatat ttgccaacaa gcctaagcct gcctttcgca aactccacac tttattgata
     7681 tcattacctt cgattaatgg atattgataa cccaaatttt ctttttctaa caacaaaaca
     7741 aattgttcag ccattttttg tgcataagca gtatcgtctg atgaaaattc aacacacaaa
     7801 attgcttcag gatcgccttt tacaaaaaat ctattttttc gatgttcaat attcgctttg
     7861 gtacaatcca aaataacttt atccatcaac tcacatgctg aaggagcaaa tttcatagca
     7921 aaaactgttg ctttcaaact ctcttcgagc gtttgaaaat gtgctgcaac aactatttta
     7981 tattttggtg gagtaggtac taaatttagt tttatttcag tgataaacat caaagtacct
     8041 tcagagccac aaattagttc agctaaatta aatgctcgac ctgtaggttc aaatggagtt
     8101 tgttctaata aaacatctac ggcatagccc gtatttcttc tatgtatgct ttcctttgga
     8161 aagttttcct ttatttgagc ttgagtatct ggattgctta aattatcaaa aaggaaacga
     8221 taaatttcgc cttctctatt cttttgttga attttattat ctatctcatt atcatctaaa
     8281 gccttgatac ttatggcatc tccattgctc aaaaaccctt ttaattccaa aacatggttt
     8341 ctagtagagc catatactat tgaattagct ccgcaagaat tgtttccaac cattcccgca
     8401 atcatagcac gattggcagt agaagtttca ggtgcaaaat ataatttata gtctttaaga
     8461 taataattta attcatcacg aataacacca ggttcaaccc ttacccaact tccttcagta
     8521 ctaacttcta taactttatt caaatgtttt gaaatatcaa cgacaatgcc atttcccaca
     8581 acttgaccac ctaaagaagt accagcagct ctcggaatta aagcaacttt atattgattg
     8641 gaaaaatgaa tgagcttttg gatatcactt tcgttttttg gataggcaac cgccaatgga
     8701 tattctttat atacagaccc atctgttgcg tataagattc tcatcttttc atcaacaaat
     8761 aaatcacctt ccaactgtgt ggctaaacca tccaaaatgc ttggatcgat ttgatatgga
     8821 ttgacaatag tgctcatgtt tacaccaaaa ttacaaaaaa ataaaagttc tttgtattat
     8881 acgcaagtta tatttaattc gttttaatat taatcaaatt aagcttgtgt tgtcggtgtt
     8941 ccaaagttca ttggcaacgc cactgcttca ttgtctttga tttcaccatt caattgttca
     9001 aatttcttga cattatcttg taaagccttc atcaaacgtt tggcgtgttg aggagtcaaa
     9061 ataattctag attttacttt tgctttagga atacctggag caactcttat aaaatccatt
     9121 acaaactctt ggtttgagtg tgtgataatt gccaaattgc tgtattctcc ttcagcaata
     9181 tcttctggta gttcaatatt aatttgatta ttattttcca taatatgaat atttgtatta
     9241 aaaaaaaggt tgtctttcga caaccttata aagatagtta aaaattttaa tcttcgtctt
     9301 cttctaggaa tgctttttta accaatttat catgttcttt tttgtttgct acattaattt
     9361 cttggaaagc cttaacgcca gttcctgcag aaattctatg accaacaata acgttctctt
     9421 tcaaaccttg catataatca atagaagcat taattgcagc tctatttaat actttagttg
     9481 tttcttggaa cgatgctgcc gaaatccagc tatctacacc taaagaagat tttgtaatac
     9541 cttgtaataa tggacttcct gttgcaggta aagcatctct atattctaaa gtttttaaat
     9601 cattcttttt caggttagaa ttttcttctc taatttgacg catagtaacg atttgtccag
     9661 cataaagttc ttgagaatct cctgcctctg taatcacttt cttatcaaaa atatcgtcgt
     9721 ttacttttac aaattcaaat ctattaactg tatcgccttc taaaaattta gtatctccag
     9781 catcttcaat caataaatta ctcatcattt gtcttacaat tacttcaatg tgtttatcat
     9841 tgattttcac accttgaagt cgataaactt cttgaatttc gtttactaag taagcttgta
     9901 aagcacctgg gcctttgata tttaaaatat cagctggtgt gatagaacca tcagacaatg
     9961 gcatacccgc ttttacgaaa tcgccatctt gaactaaaat atgttttgtc aaagaaacta
    10021 agtatttctt tcttacacca tcttttgaag taacagtgat ttctctattt cctctcttaa
    10081 ttgcttttga gaaagtaact tgaccatcaa tttcagaaac ttttgcagga ttagaaggat
    10141 ttcttgcctc aaataattct tgaactcttg gaagacctcc cgtgatatct cgtactttac
    10201 ttaaaattct tggaatcttc actaagatcg taccagtatt aacagcaaca ttgtctttta
    10261 caataatgtg gctaccaact ggcattgaat aagaagccaa ttcttttcct ttatcatcaa
    10321 ctactttaat tgcaggtgct tttttcttat ctctcgattc gatagttacc cattctatgt
    10381 ggcccttttg ctcatcaatt tcctctctat aagtaacgtt ttctatgata tcttcaaact
    10441 tcacagttcc tttaaattca gaaactacca cgttattata tggatcccaa gaacagatta
    10501 catcgccttt tttaactttt tgaccagatt ttacagccaa aattgaacca taagggatat
    10561 tatttgtgat gagtactctt ccagattctt catcaacgat attaatttca cctgttcttc
    10621 cgataattac ttgttttact gatccgtctt cagcttcttg ttttacagat ttaacatcat
    10681 caaattctaa cttaccatca aatttagcta acaagcttga atctgcggca actgatgcag
    10741 cagcaccacc cgcgtggaat gtacgtaaag tcagctgcgt acctggttct ccgattgatt
    10801 gtgccgctat aataccaaca gcatctccca tctgagtttt ttgattattg gttaagtttc
    10861 ttccataaca ttttgaacaa acacctctat ctaattcgca agttaataca gaacgaattt
    10921 ctacttcttc aattccagat tcttcaattt gcttagccgt tgcttcattg attaattctc
    10981 cttctgaagc aattatttct ccagaaactg ggtgtacaac atctaataaa gctgttctac
    11041 caacaattct atctgataaa gattcaatga tatcatctcc ttctttaagt gcggcaacat
    11101 ttaatcctct taaagtacca caatcttctg cattgataat tacatcttgg gctacatcta
    11161 ccaatcttct tgtcagataa ccagcatccg ctgttttaag tgcagtatcc gccaaacctt
    11221 ttctagcacc gtgtgtagat ataaagtact ccaatacact caatccatct ttgaaattag
    11281 aaaggattgg attttcaata atatctccac ctgcagaacc agattttctt ggtcttgcca
    11341 tcaaacctct catacctgcc aactgcttaa tttgttgttt agaacccctc gcaccagaat
    11401 ccaacatcat ataaactgaa ttaaatcctt gttttgtaga tgaaatttct ttcatcaatc
    11461 cttcggtaat tcttgtatca acacgagtcc agatgtcaat aatttggtta taacgttcgt
    11521 tgttggtaat taatcccgta ttgaaatttt gccaaacatc ttccacttct aataaagctt
    11581 cattcaacaa gttttgtttc aaatctggaa taatcaagtc atcaatattg aaagataaac
    11641 cgcctttaaa tgcttgacgg taacccattt ctttaatatt atctaagaaa tttaccgttg
    11701 ttggaatatc tgttgtcgtt aaaacatttc caataacatc tttcaagttt ttcttagtta
    11761 aaagtacatt gacaaacccg caagatgcag gaacattttg attgaagata attctaccta
    11821 aagtagtttc aataatttta tctaccaatt tgccattctc taatactttg gtttttactt
    11881 taacccaagc atgaatatct gctttcttct cattcaatgc aataatcgct tcttccgcag
    11941 aataaaaaat tcttccttct cctagaactt tctccgtttc tgttgttttt ttaccttttg
    12001 tcaaatagta tagaccaagc accatatcct gagaaggaat agtcattggc gaaccatttt
    12061 gagggttcaa aatattatga gaagataaaa ctaataattg agcttctaag atagcagcat
    12121 ttccaagagg aacgtgaact gccatttggt caccgtcaaa atcggcgttg aatgctgttg
    12181 ttactaatgg atgcaattgt attgcttttc cttctattaa ttttggttgg aaagcctgaa
    12241 tagataatct gtgtaaagtt ggggctctgt tcaataatac aggatgtcct ttcattacat
    12301 tttccaagat atcccaaact acagattctt ttttatctac taatttttta gcagatttta
    12361 cggttttaac aacgcctctt tctaataatt ttctaatgat aaacggtttg aataactctg
    12421 ccgccatatc ttttggcaaa ccgcattcgt gcatttttaa ttctgggcca accacaatta
    12481 cagaacgacc tgaataatcc acacgttttc ctaataagtt ttgacggaaa cgaccttgtt
    12541 ttccttttaa accatcagat aatgatttta atgcacgtcc tccatcggct tttacagcgt
    12601 tagattttct tgagttgtca aacaatgaat ctactgcttc ttgaagcatt ctcttttcat
    12661 ttctcaagat aacttcaggt gctttaattt caattaatct ttttaaacgg ttatttctga
    12721 taataaccct tctatataaa tcgtttaaat ccgatgaagc aaaacgacca ccatctaatg
    12781 gaaccaaagg tcttaattct ggtgggatta caggtaaata atgaacaacc atccattctg
    12841 gtctgttttc tgtatgttca ttggcttctc taaaagcttc tacaacactt aatcttttta
    12901 atgcttcaga ttttctttgt tgagatgttt cattcgatgc actatgtctt aattcagcag
    12961 caagtgctgt taatttgata tttgctaata aatctctaac gccttcacca cccattttgg
    13021 cgataaattt atttggatct tcttctggta ataataaatt ttcttttggt aaagcatcca
    13081 ttacatctaa atattcttct tcacttaata agtcaaggta tttatatgtt ttcttttcac
    13141 catttatcat aataccatct tccaaaatac ctgcttggat aacagcatat ctttcataat
    13201 agatgataga ctctaaattt ttagaagata atcctaaaac ataaccaatt ttatttggca
    13261 acgatttgaa ataccaaata tgtacgatag gtacaaccaa tttgatgtga cccattctct
    13321 cacgacgtac ctttttctca gtaacttcaa caccacatct atcgcaagta atgcctttat
    13381 aacgaattct tttgtactta ccgcaatagc attcataatc ttttgaaggt ccaaaaatac
    13441 gttcgcaaaa tagtccgtcc atctctggct tatacgttct atagttgatc gtttctggct
    13501 ttttcacttc tccacaagaa cgactaagaa taacatctgg agaagccaac tttaaagtaa
    13561 ttttattaaa ttcggcttta actttactat ccttctttaa aggcataaat aatttttttt
    13621 agattaaaaa atattttaac agaaaaacta tgcacaaagt acatagtgag gtgcaaaagt
    13681 acaacttaaa aattagaata cataaaaaag aagtagattt tttaggttaa ttaaaaatta
    13741 agttcaacga acctctttca gattaaataa tctttatgtt ttaaatctat tctacggtaa
    13801 cgctctttgc caagtttctt ggttgatcta cattacagcc tctcataact gcaatgtgat
    13861 aagccaataa ctgtaagggt acaaccgaaa gaataggaga caaagcctct aaagtatttg
    13921 gaatttcaat aacatattct gctaaagctt caacttctgt atctccttca gtaacaacag
    13981 caataattct accttttctt gttttaactt cctccacatt tgaaattact ttttcatagt
    14041 gttggcaatt tggtgccaat accacaactg gcatattttt atcaatcaaa gcaatcgggc
    14101 cgtgcttcat ctcagcagct ggatagcctt cagcgtggat ataagaaatt tcttttaatt
    14161 ttaaagctcc ttccaaagca acaggaaaat taaaccctct tcctaaatat aaaaaattac
    14221 ttgaatcttg aaaagcatct gcaatctctt taatatatgt atcactttct aaaattttat
    14281 cgattttaga aggtaatata tctaattcat ttaaaaattc attgagttta gacgacgaaa
    14341 ttgtacctct taattctgct agttgcaatg ccaataatac taatatactt acttgtgttg
    14401 taaatgcttt tgttgatgct acaccaattt caggacccgc atgtgtataa gaacccgcat
    14461 ccgtcattct cgctatgctt gaacctacaa cattacaaat gccataaata aaagcccctt
    14521 tttgttttgc taattctaaa gctgctttag tatcagcagt ttctccagat tgagaaatag
    14581 caataacaat atctttttcc gataatactg ggtttctata tctaaattca ctagcatatt
    14641 caacctccac attcactctt gccaattcct caattaaata ttctcctatc aatccactat
    14701 gccatgaagt accacaagca acaataatta tacgttccgc atttttgaag ctactttcaa
    14761 atttattaat accacctaaa cttattttca tttgctctaa actcattcta cctcgaatgc
    14821 tgtttaaaat ggcttgtggc tgttcgtgaa tttctttcat cataaaatgt tcaaatccac
    14881 ctttttctat agcttcaaga ctaatactaa gttcttctat ataaggactt tgaacagcat
    14941 tgtcttttaa gttctttatt tctatttcgc cattcaaatg caaaacagcc atgtgaccat
    15001 cttcaatata acttacacgt cttgtgtatt ctattaaagg cgaaccatca gatgcaacat
    15061 ataattctcc atcgccaaat ccaattacca ttgggctact cattttggca accactaatt
    15121 tatcgggttc ctttttggaa ataacaacaa tcgcataagc tcctacgact tcattcaaag
    15181 ccaaacgtac cgcctctgct aaatctacgt gtacattttt ttggatatca tcaataaggt
    15241 gtgcaacaac ttctgtatct gtttgtgatc taaactgata gcctctttgt tctaatgctt
    15301 tttttaaagc attgtagttt tcaataattc cattgtgaat aattgcaata tcaccatctg
    15361 tactgatgtg tgggtgggca tttacatcat ttgcttctcc atgagtagcc catcttgtat
    15421 gaccaatgcc aatattccct tctatattat ttgctaaggc aaaagcttcc atctctacca
    15481 ctttgccttt tttcttatag acgtttagat tatgttcttc atctaaaatt gctactccag
    15541 aactatcgta acctctatat tctaaccttt ttaatccatt tactataatt ggaaaagctt
    15601 tcttctttcc tatatatcct acaattccac acataaattt ctaaattact gctttgaata
    15661 aaatattttt aatcttgttt tttgataaat atcgctacta ttagttcctc caatggttaa
    15721 tttcgtagga gcataaccac caagataaga aatatcattt aattctgcat ctgtattttg
    15781 attaatgttg tggttcgata aataaatatc tcttgttgtt aatctgccta accacatttc
    15841 tttaacaata gttgaaatat taatattata tcgagctact ttttcattat ttccatttac
    15901 ataaacagag tcttttgcac caattccgga aattttaaaa ttccctgaat tatctttata
    15961 taatgcataa ataatagaaa cattggaact tgtatcagca tttgaattgg tctgagtaac
    16021 atataactcc gctttattaa ctgccgcttg ctgatttatc aaattagaaa aatctggaat
    16081 agtaatttta ttcatcaagc cagcttgtcc gatgatgaaa ttaatactat ctgttagttc
    16141 agtagagctt aatgcattat ttactgatga attatatgta ttgccataaa catttatagc
    16201 tgaagattgt agataagtcg tactttcttt aactacactt gttaaacgag gatacatatt
    16261 catttctttt ctaacaccat ttttatcttt taaaaagatt gtaaatctgg ttttatcgct
    16321 ggttaaatct actaatgcca ttgcattgcc taaattattt tcaaaagcaa cttctaatcc
    16381 tctaaaataa ttatcaaaat tggcatattt tattaaagca ctgtctgatg agcctatctt
    16441 atctacataa gtttgagcat agcttgcctc cattggtact cgaattatag aaacagaacc
    16501 taaagtgtct ccagtataag tgaaaaattt ttgacttggt ttcaatgcta tattatttgc
    16561 tcttccgact tcatttgtgc caaaaaaaat agcattatca gaatagtatt tttgattttc
    16621 taaaatatta tcattcaatg ttttaaccac aatattttga gttgaatttg tatcgccata
    16681 aacaatattg tatttcataa ataatactgc cgaatcaatc gtataaggtg ctaaagaatc
    16741 tgctaaagct attgttggat ttgccaattc aaacattaat ttggcatgat ttttcccata
    16801 ttcagcactt tgcaacaccc ctatataatt aaaaatcaat ttgtctgttc ttacaggcga
    16861 ttctgctatt gtctttgatg ttaataaaaa agtatctgaa agttctaaat tcaaataatc
    16921 tgaattgggt aaaatatctc cgcctatatt gtctgctttt ttacaagaaa agattgctgt
    16981 aaaagctaaa aggattaaaa agtattgctt catgtatatt atccaataat ttggttgtaa
    17041 aaatctaaat atttttgttg gatatcaata tcttcttgat atgctaacca aggttttttt
    17101 gcagcgagtt tcttaaaagt atccacatca ctcaactctt cagaaccaat gcttatggca
    17161 tcagcaaatt ttatacctaa tttatttaaa catacattat ttaatccttc taaaagttgt
    17221 atgtctttat cttttatttg ttcactaatt tttgcttttt tcaaaaatcc attattcaat
    17281 tcttcttgaa aatcttgttc gtacaaagaa taaataattt tactgtttgt aaaaattgga
    17341 tctttagcat aatgtgttct caaatacatt ggtatcaaac ttgtaaacca accatggcaa
    17401 tgaataatat caggtgtcca gccaaatttc cgtactgtct caatcaaact tttacagaag
    17461 aaaatcatac gctctccatt gtcttccaag aaattttctt cttcatctct gaaaactaat
    17521 tttcttttaa aaaattcttc attatctaaa aaataaactt gtattcttgt tccaggcaag
    17581 gacgctactt taatgattaa tggataatct aaatcgtcaa taatgatatt tataccagaa
    17641 agcctaacca cttcatgcaa tcggtttctt ctttcattga ttactccaaa acgtggcatc
    17701 agcactctca cttcccagcc attgtctgtc gccattggag ctaatctttt aaccaaactt
    17761 gccatttctg tcaatgccaa ataaggatcc atttcttgtg ccacaaacaa caatcttttt
    17821 tgttccatac gctacttcct caattttttt tcttaatttt taaaaatctg cacaaaggta
    17881 cgaatattat gtgttaatat tgcttatttt ttgaaaatga aagtatttag ctctataatt
    17941 gaaatcagaa aatttttaga cgaacagtct gaaagtcaat taataattgg atttgttcca
    18001 actatgggtg ctttacatca aggacattta tcgcttattg agcaatctaa aaaaagttgt
    18061 gatattacta tttgcagtat ttttgtaaac cctactcaat ttaacgataa aaaagatttt
    18121 gaaaagtatc ctattcaaat tgatgctgat ttagaaatgt tgattgcagc tggttgtgat
    18181 gttgtattta ttccttcggt agatgaaatg tatcctgaag gaactacaca agataaacta
    18241 cttgttgatt taggattttt aggaaaaact ttggaagccg aaaa
//
LOCUS       DAOQCW010000041        17870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1120_length_17870_cov_5.640472, whole genome shotgun sequence.
ACCESSION   DAOQCW010000041 DAOQCW010000000
VERSION     DAOQCW010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 17870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17870
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1120_length_17870_cov_5.640472"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(285..893)
                     /locus_tag="PLS16_05215"
     CDS             complement(285..893)
                     /locus_tag="PLS16_05215"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNJ60512.1"
                     /translation="MITIILITITALISFAAFNNHSITYNYIFNANKIYKDKQWYRFF
                     SSGFLHADMFHLFFNMYALYLFGRSVENFLLLKYGDLFGKILYLFLYISAIAVSSIPD
                     YKKYKFSPSYNALGASGAVSAVIFVCVILYPNQGIMIFPIPFFIPSYIFGPIYLAYSY
                     YMAQRNMDNIGHNAHFFGALYGIIFIFVIYKDALQNFLHQIL"
     gene            complement(895..1329)
                     /locus_tag="PLS16_05220"
     CDS             complement(895..1329)
                     /locus_tag="PLS16_05220"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HNJ60513.1"
                     /translation="MPENKIKIKQIDGVTFIRCLVRKKWIQLTPEEKVRQFLIHYLSE
                     ELYYPIKYISIEKKIVINQLSKRYDIVVYNYELQPVILIECKANHIKLNPENLQQLLN
                     YNILLRTPYLMLSNGTEHLIYQLNENSMDRIKQLPDKSVIFN"
     gene            1407..2405
                     /gene="holA"
                     /locus_tag="PLS16_05225"
     CDS             1407..2405
                     /gene="holA"
                     /locus_tag="PLS16_05225"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNJ60514.1"
                     /translation="MTFESINKEINHRKFQPIYFLFGEEDYYIDKIEQQILEKALEPH
                     EKDFNLDIFYAKDIDSVAILNVVNQYPSFAERRVVLIREAQEFKSKVWEELESYFTKP
                     NATTILIFCHKHKALDKRTKIAKTIEKNSVFFNSEKIKEEKLPQWISQFVSANNLKIA
                     DAEAQLLAENLGNDLSRISNEIEKLKVVLPPNAPITKEAIEKWIGISKEYNLSEMNKA
                     VIQHDFGKAIKIALYFEKNPKAGSTIPIIAYLYQFFSRLLVFHAHKNKSDNEFRSMGL
                     FFVQDYRNGARFYSNSKTIEIIHLLNEFDAKSKGLYASSNTSNPDLLRELIYKIMH"
     gene            complement(2646..3287)
                     /locus_tag="PLS16_05230"
     CDS             complement(2646..3287)
                     /locus_tag="PLS16_05230"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNJ60515.1"
                     /translation="MAQRSAYENDLKYQRKKFHFGIHLGIGMSDFRIRHSNAFALSDS
                     VLSVTSKLTPGYEIGALASYHLSKNFELRVLPTFLFNDKNLTFKYKDETTQKLKLSTI
                     VFDMPVEIKFKSDPIKDFKVYVVAGMKYSYDLGTKLSGRFVTYMPQQKPNDLAVNYGV
                     GFEFHFPLFILSPEFKVSNSILNIHQPTKDFTQSNMIQRLYNRTFLFSLNFEG"
     gene            complement(3329..4051)
                     /gene="ubiE"
                     /locus_tag="PLS16_05235"
     CDS             complement(3329..4051)
                     /gene="ubiE"
                     /locus_tag="PLS16_05235"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HNJ60516.1"
                     /translation="MKQTVTPYNSSEGKKKQVANMFNNVAGTYDFLNHFFSLGIDKLW
                     RKKLVKLISREQPLLLLDVATGTGDLAIAESVIPNIQITGIDISEGMLEVGKTKVKQK
                     QINNIQLKLGDSENLEFATDSFDAVSVAFGVRNFENLEKGLLEMQRVLKPGKKLFVLE
                     FSKPSNFIFRSIYYLYFFYILPFVGRIVSKDSSAYSYLPESVKNFPSGKEFVEVLQKC
                     NFKEIECKTLFFGISSIYIARK"
     gene            4152..4769
                     /gene="yihA"
                     /locus_tag="PLS16_05240"
     CDS             4152..4769
                     /gene="yihA"
                     /locus_tag="PLS16_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNJ60517.1"
                     /translation="MIAKATFVGSFNKETECPKVEIPEFAFIGRSNVGKSSLINLVTN
                     RKDLAKTSQTPGKTQKLNFFLIDDTWHLVDLPGYGYAKVSKTMRSDFEKMIQYYFKNR
                     EQLYCTFLLIDASISPQKMDIEFANWLGQHQVPFVIVFTKVDKAKALEIKKNIDKFNG
                     ELLQTWTTLPQSFKTSSERKIGQRELMDFIQLIVKESKNQPSNTK"
     gene            complement(4762..6195)
                     /locus_tag="PLS16_05245"
     CDS             complement(4762..6195)
                     /locus_tag="PLS16_05245"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNJ60518.1"
                     /translation="MTTHEHQLIQLFEQYYKITPTLILQLPQAGSDRIYFKLSAENIE
                     AIGCWGNDIKENQTFIHHALHFKKLHINVPEIYAISENQAYYLQEYLGDISLYDIIKK
                     EGLVEHTINYLKNTLKSLAFLQIKGAENFDFNTCFPIQEFNRSSMFWDLNSFKYYFVR
                     MARVQFNELALNKDFHTLCNFLLEEKHLYFMFRDCQSRNVMIKDDQPYFIDFQGGRKG
                     ALQYDAASLIWQAGAKIPYPIRAELLEYYTNEVCQLIALDKNEFKEKYYGFVLIRILQ
                     TLGAYGFRGLIEKRPHFLQSIIPALDNIKWFLGNIELKIDIPELKNILLQLVNSPLFK
                     PEETEKKSSLLQIEINSFSYRRGIPEEQHGNGGGFVFDCRGILNPGRFEPYKQLHGKD
                     KAVIDFLENKTKVHEFLEGVWKTIGINIDDYIDRDFGHLQINFGCTGGQHRSVYCAEQ
                     TAKFIEKKYGILPKIRHIERENNGQFI"
     gene            6278..7417
                     /locus_tag="PLS16_05250"
     CDS             6278..7417
                     /locus_tag="PLS16_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNJ60519.1"
                     /translation="MDFNQTENQILITQTIRDFAEKHIKPYMMEWDETQHFPVETLRA
                     LGELGLMGIVIPEEYGGSGFGYFEYITALTELGKVDGSLTLSVAAHNSLCTNHIFKFG
                     NDAQRRKWIPKLASGEWIGSWGLTEPNTGSDAGRMQCVARQDGDDWIINGTKNFITHG
                     ISSNVTVVIARTGELLDSRGMTAFVVERGTQGFSGGKKENKLGMRCSETAEMIFDNCR
                     IPKENVLGNVGDGFVQALKILDGGRISIAALSLGIATGAYEAAVQYSKEREQFGKSIS
                     TFQAIAFKLAEMHTKIEAAKLLTYQAADMYMRGENINKESAYAKYYASEVAVYASTEA
                     VQIFGGYGYIKDFPVEKHYRDSKLCTIGEGTSEVQKLVISRAILK"
     gene            7554..7964
                     /locus_tag="PLS16_05255"
     CDS             7554..7964
                     /locus_tag="PLS16_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60520.1"
                     /translation="MSIIYRNIVLLAFCLITNFIFGDNPEGQEYDKEKIKNRSASIWR
                     HEINYPPLTTKSGLRSQIFFLILYDDGTYEQQVFIIPEDKDAYKGIKYYQLSGKWSSN
                     NKEISLDEYGKTQKISNNDFYTKFENIDNLIVKK"
     gene            7982..8950
                     /gene="argC"
                     /locus_tag="PLS16_05260"
     CDS             7982..8950
                     /gene="argC"
                     /locus_tag="PLS16_05260"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNJ60521.1"
                     /translation="MEQINVGIIGATGYTGGELIRVLLQHPKVKIDFCYSRSKFGKKI
                     SDIHQDIFYSDLTFTNEIYPNVDCVFLCLPHGDTKPFLKEHQFSASTKIIDLSNDFRL
                     KADAGEFVYGLPELNKEKIKTANKIANPGCFATTIQLALLPLAKNQLLNGDIHISAIT
                     GSTGAGNKLSETSHFSWRNNNMSTYKTLTHQHVGEINESLMQLQANYNQSLLLVPYRG
                     DYPRGIIATSIISTDESIENIRTLYESFYKDAAFVRLSDTNINLKQIVNTNYCLIYLE
                     KQGSKLIVVSASDNLLKGASGQAVQNMNLIFGLDEKMGLNLKPTGF"
     gene            complement(8951..9448)
                     /locus_tag="PLS16_05265"
     CDS             complement(8951..9448)
                     /locus_tag="PLS16_05265"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNJ60522.1"
                     /translation="MKYIITTYMALFSLFLLSTSSVQAQNIFKVTENQLILPNPILFD
                     LHANDISDTNNPIIDYVADFLKQNSNISKFRIESHVFTESNSTENMKLSMQRAAIVAY
                     YLTTKGIDCGILTVSGFGDTNPIDSINTNLNTRVDFYIEKLGNSEVATKRIFNTCKFF
                     NPCDE"
     gene            complement(9530..11197)
                     /locus_tag="PLS16_05270"
     CDS             complement(9530..11197)
                     /locus_tag="PLS16_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNJ60523.1"
                     /translation="MKWQNFFRTKSIQHILDEVKSSPELQESQLNKNLTVRDLAALGI
                     AAIVGAGIFSTIGSACFDGGPAVSLLFLGIAIACGFSALCYAEFASRVPVAGSAYTYA
                     YTTFGELIAWIIGWDLILEYAIGNITVSIAWSGNFVKFMHDCGVYLPAYLTTSFLEAK
                     KYIPELYAAAPKLFGIPFIINIPAFLINFFITTLVYMGIKESKNFANGMVVFKVAVIL
                     IIIIIGLFFVKPENWSPFAPNGFNGVIKGVSAVFFAYIGFDAISTTAEECKNPQRDLP
                     KGMFYALVIATVLYILVSLALTGMVHYTQLNNEAFLANAFTQIGMNWMGGIIAFSALI
                     ATASVLLVFQIGQPRIWMSMSRDGLLPKKLASVHPKFKTPGFATIITGIIVGIPSLFL
                     DVNLVTDLCSIGTLFAFALVCGGILILQHREKNEGLENQAKFKVPYINGRIMMVILWG
                     ILLLIFLINNEAKRYIDTIFSLEGGFSVWHHHIPIWIFWSIALVISGYTFVKKWSAIP
                     ILGFLSCSYLLSTLGVLNWLRFIVWMLIGLIIYLVYGYKKSKLNSNT"
     gene            complement(11229..12758)
                     /gene="dnaB"
                     /locus_tag="PLS16_05275"
     CDS             complement(11229..12758)
                     /gene="dnaB"
                     /locus_tag="PLS16_05275"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNJ60524.1"
                     /translation="MSEFTTSKKTRTNRTKNEDISHLLYGKVPPQARDLEEAVLGAIL
                     IEKDAISYVSDILKPESFYVDAHGKIYRAIQNLFARSQPIDLLTVSEELRKEGQLESV
                     GGAYYLSELTNKIGSSANVEFHARIVIQKFIQRELIHISNDIIKDAYEDTTDVFDLLD
                     GAEKRIYEITDKNLRNATQGIGQLVSKSVMQIDELISNDGGVIQDSVPSGYKDLDNFI
                     FGWKATDLVIVAARPSMGKTAFVLNVARNAAVDYNLPVAIFSLEMGGVQLAKRLISME
                     TEIDAQKVTNGKMTEAEFAHLRDKVERLAKAPIYINDQPAINVYELRAQCRRLQNAHG
                     IKMVIIDYLQLMSGGGDKGMNREQEISNISRSLKGLAKELNIPVIALSQLNRSVETRG
                     GDKKPQLSDLRESGSIEQDADMVMFLYRPEYYKIDEDKDGASLKGTAEIIVAKHRNGP
                     VGSVQLRFQNNYGRFYDLDRGAGVLDELNNKSEYKIIPSKANFDEENDHNSSDEKFDL
                     F"
     gene            13034..13648
                     /locus_tag="PLS16_05280"
     CDS             13034..13648
                     /locus_tag="PLS16_05280"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNJ60525.1"
                     /translation="MYFVLVKEKKELIQLEVLNITQGFSQYNSYSVVLGEKNGNRRLP
                     IVIGAVEAQSILIAKENIPSKRPLTHDLIKTMFGKFDVMPLEVVINHLLDGVYYAIIT
                     VVYDGYVIDIDSRTSDAIAIALRCNIPIFIHKDILEDVAITFLKSEDEIISISENPEE
                     EYLLQKQSSFSQYSVVELESLLKEALQNEDYEKAAKIRDELKRK"
     assembly_gap    13838..13937
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13938..14597
                     /locus_tag="PLS16_05285"
     CDS             <13938..14597
                     /locus_tag="PLS16_05285"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNJ60526.1"
                     /translation="SSIGVASGIDRKKSIPDEQTPLIKTGVYYDDAKIDTETLVIDFC
                     TQNNLFYTIVRPSAVVGPGSVWVKEPIERKLHKGFFPLINDGRESACLLDARNLAEGI
                     YSIITKEIAKNNIYYFMDDFKDISWKRYFTDLLKMVNQKPSISIPYSVLYPIAVLMEV
                     IANFTHKKPMIAKKSINVLGTSRLVHTQKAKDELGYKSIYQYEETMQYIQEWVKNYYK
                     I"
     gene            14663..15547
                     /gene="era"
                     /locus_tag="PLS16_05290"
     CDS             14663..15547
                     /gene="era"
                     /locus_tag="PLS16_05290"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNJ60527.1"
                     /translation="MKHKAGYVNILGKPNVGKSTLMNALIGEKLSIITSKAQTTRHRI
                     LGILNGDDFQMVISDLPGIIKPAYKMHQTMMQFVKTSLEDADVFIYMVEIGDKPEKQP
                     EEYEKIKQLNVPVIILLNKLDITDHEIVEYERELWEADFPKAEIISFSAKFNWNLEKL
                     MDFALKHLPESPPYFDKDAITDRPERFFVTEIIREKILLYYKQEIPYSTEVVCTYFME
                     DGEIIKIAAEIYVERASQKPILIGKNGEMIKKVGIEARKDLEEFFQKKIYLETFVKVA
                     DGWRNSDNMLRRFGYRDY"
     gene            complement(15557..>17870)
                     /locus_tag="PLS16_05295"
     CDS             complement(15557..>17870)
                     /locus_tag="PLS16_05295"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNJ60528.1"
                     /translation="YFYPKDELILDAKYMQINDIILLNNQKNQYLSYNYDSLQLKIYL
                     GKQFTSSDTLTILIDYIAKPYSISESVLEIDGRGMYFINAENKNPYKPFHLWTQGEVE
                     ANSFWFPTIDAPNEQCSQEIFVTLDTAYISLSNGILVSSTNKGNKRIDYWKQDKQHAP
                     YLFFLGIGDYKKYHTTWRNIDVDYYTFKDYYKDVQEVFGNTPEMLTFYSNLLKVDFPW
                     DKYAQIIAFDYTAGAMENSSAVILYDKLLMTHQDLIDDNYDFIIAHELFHHWFGDLVT
                     AESWANLTLNESFADYGEFLWANYKYGKDNGDAVNKDAFEKYLKQSKYKNEPLVDYYY
                     KAHKDMFDGIRYEKGGRILHLLRNYIGDDAFFKALNLYLVQNQFNSAEPSDFRKVIES
                     VTGKDWNWFFNQWYFDKGHPILDIQNTYHSETKNIEVKIKQIQDKNTAPIFKIPTTID
                     IYFNDSILRKELSIINKESTFYFPAKTKPLFINFDPEGILPAELKENLTESESALKFD
                     RANSYYARSRVLNSFVNNIKGNKTAQDIFIKAIQHTDWNTQKEALSLIYSPSDFTDKN
                     LLNTVLKELIYQSSKADIRKMALNLLNKNNSELSFAVAQYVIKKDSSLQCIAAALDIV
                     KTKNFAIAYQEALPYLDTKNPTLLRSIGSILKDTTVNHLDFFKKAIWLNNYRNAYQNF
                     ENLSDFLMQTQDENLFQDAINFLSDINHYEESNFNIDGSIRICISLRNKLEYQQNNKK
                     TDKYLLPIIAKKLEILNKAIPQLKGNNLGF"
BASE COUNT         6362 a         2629 c         2622 g         6157 t
ORIGIN      
        1 tcgaaccccc gacccgctga ttacaaatca gctgctctgg ccaactgagc tacacaggca
       61 atttatttca acacactaaa agaacaactc caaaatgtaa atcacatttt cggagtgcaa
      121 atatacaact taattttgat ttataaagca ttctgttgaa aaaaaaatcg ctttataatt
      181 atttaaaatt aacttttgca aatttatttt tcatattgtg tttaaaatta cactatctat
      241 ttgataatta attatttata ttgtctataa tcggtaattg ttttttataa aatctgatgt
      301 agaaaattct gcaaagcatc tttataaata acaaaaataa atattatacc atacaatgct
      361 ccaaaaaagt gtgcattatg tccaatatta tccatatttc tttgagccat ataataggaa
      421 taggctagat aaattggtcc aaaaatataa gatggaataa aaaatggaat gggaaaaatc
      481 ataattcctt gattgggata caaaataaca catacaaaaa taactgccga aactgctcca
      541 cttgctccta aggcattata actaggacta aatttatatt ttttatagtc gggaatacta
      601 cttacagcaa tggcacttat gtataaaaat aaatataata ttttgccaaa taaatctcca
      661 tattttaaca ataagaaatt ttctaccgaa cgcccaaata agtataaggc atacatattg
      721 aaaaagaggt gaaacatatc ggcatgtaaa aaccctgaac taaaaaaacg ataccattgt
      781 ttatctttat agattttatt ggcattaaaa atataattat aggttatact atgattattg
      841 aaagcggcaa agctgattag tgccgtaatt gttattaata ttattgttat cattttaatt
      901 aaatatgaca gatttatcgg gtaattgttt aattctatcc atagaatttt cattaagttg
      961 atagattaaa tgctccgttc cattactcaa catcaaatag ggtgttctaa gcagtatatt
     1021 ataatttaat aattgttgaa ggttttctgg attaagtttt atgtgatttg ctttacattc
     1081 aataagtatg acgggttgca actcataatt ataaacgaca atatcataac gtttactcag
     1141 ttggttaatc actatttttt tttctattga tatatatttt attggatagt ataattcttc
     1201 agacaaataa tgtattagaa actgcctaac cttttcttca ggcgttaatt gtatccattt
     1261 ttttcgtacc aaacatcgta taaatgttac gccatcaatt tgttttatct ttattttatt
     1321 ttcaggcata atttctaaat acaaggcttt tttaatattt tagcaaaatt agttttttat
     1381 taaatactac ctaattattt ttaagaatga cttttgaatc tatcaataag gaaatcaacc
     1441 atagaaaatt ccaacctatt tatttcttat ttggtgaaga agattattat attgataaaa
     1501 ttgaacaaca aattcttgaa aaagctttag agcctcacga gaaagatttt aatttagata
     1561 ttttttatgc aaaagatatt gactccgtcg ctatcttaaa tgtagtcaat caataccctt
     1621 catttgcaga acgcagagtc gtattaatta gagaagctca ggagtttaaa tctaaagttt
     1681 gggaagagct tgaaagttat ttcacaaagc caaatgcaac aactatactt attttttgtc
     1741 ataagcacaa agctttggac aaaagaacta aaattgcaaa aactattgaa aaaaactctg
     1801 tattctttaa ttctgagaaa ataaaagaag aaaagttacc acaatggata agccaatttg
     1861 taagtgcaaa taatttaaag attgctgatg cagaggcaca attattggct gaaaacttag
     1921 gaaatgattt atctagaatt agtaatgaga tagagaaatt aaaagttgtt cttccgccaa
     1981 atgcaccaat tacaaaagag gcaattgaaa aatggattgg tatttctaaa gaatacaatt
     2041 taagtgagat gaataaagct gtaattcaac acgattttgg aaaagccata aagattgctt
     2101 tgtattttga aaagaatcca aaagcaggtt ctactattcc tatcattgct tatttatatc
     2161 aattttttag tcgattgttg gtttttcatg ctcataaaaa taaatcggat aatgaattta
     2221 gaagtatggg tttatttttt gttcaagatt atcgtaacgg agctaggttt tattccaatt
     2281 caaaaactat cgaaattatt catttactca atgaatttga tgctaaatca aaaggtttgt
     2341 atgctagttc aaatacttct aatcctgatt tattgcgaga attaatttat aagattatgc
     2401 attgaaagat gagatgtgag atttttagaa tcatgagtca agactttttt tgagtattaa
     2461 gtagaaagtt gagtagaaag tattgagtat taagtattga gaaattcatt atttaattat
     2521 tttattactt cattattaca ttaaccactt atcactaaaa actaacaact aaaataaatc
     2581 ctgtccatca tttaatctta aaaatcaagg ttcagacaaa tagtataatt ttttttaaat
     2641 ccttattaac cttcaaaatt taaggagaat aaaaatgtac gattatataa tcgttgtatc
     2701 atatttgatt gtgtaaaatc ttttgttggt tgatggatat ttaaaataga attggaaact
     2761 ttaaattctg gagaaagaat aaacaaaggg aaatgaaact caaaacctac accataatta
     2821 acagctaaat catttggttt ttgttgtggc atgtaagtaa caaaacgtcc actcaatttt
     2881 gtccctaaat cataactata tttcatacca gctactacat aaactttaaa atctttaatt
     2941 gggtctgatt tgaattttat ttctacaggc atatcaaaaa caatcgtgga aagtttcaat
     3001 ttttgtgttg tttcatcttt gtatttaaaa gttaaattct tatcattaaa caaaaaagta
     3061 ggcaaaaccc ttaattcaaa atttttacta agatgataac ttgctaaagc tccaatttca
     3121 tagcctggag ttaattttga ggtaactgaa agaacggaat cactcaaggc aaatgcatta
     3181 ctgtgtctaa tcctaaaatc agacatacca atacctaaat gtattccaaa gtgaaatttt
     3241 ttacgttgat attttaaatc gttttcatac gcacttctct gagccatcga cgatagcaca
     3301 aaaccacaaa caaataaaag tgttaaattt attttcttgc aatatatatg gaactgattc
     3361 cgaagaaaag ggttttgcat tctatttctt taaaattaca tttttgcaat acttctacaa
     3421 attctttgcc agacggaaag ttttttacgg attctggcaa gtaactataa gcactactat
     3481 cttttgaaac aattctacca acaaaaggta aaatataaaa aaaatataaa taataaatac
     3541 ttctaaatat aaaattagaa ggttttgaaa attctaatac aaataatttt ttacccggtt
     3601 ttaaaacacg ttgcatttcc aacaaacctt tctctaaatt ttcaaaattt cttacaccaa
     3661 atgcgacact taccgcatca aaagagtctg ttgcaaattc taaattttca ctatctccca
     3721 attttaattg gatattattg atttgttttt gttttacttt tgttttacca acttccaaca
     3781 ttccttcaga aatatcaatg cctgtaattt gtatatttgg aataacagat tctgcaatgg
     3841 ctaaatctcc agttccggtt gcaacatcta gtaataaaag tggttgttct ctactaatta
     3901 gtttgactaa tttctttctc caaagcttat caatacccaa agagaaaaaa tgatttaaaa
     3961 aatcataagt acctgctaca ttattaaaca tattggcaac ttgcttcttt tttccttcgc
     4021 tagaattata tggtgtaact gtttgtttca tttttcttac ttacaaaggt acaatttatg
     4081 ttatatttgt aatcactcaa tgtattatta aatctaaaat tggaaataat acattaaaaa
     4141 ttatgctaat tatgattgct aaagctactt ttgttggttc atttaataaa gaaacagaat
     4201 gtcctaaagt cgaaattcct gagtttgctt ttattggacg tagtaatgtt gggaaatctt
     4261 ccttaattaa cttagtaacc aatagaaagg atttggcaaa aacttcacaa acacctggaa
     4321 aaacacaaaa actaaatttc tttttaattg atgatacttg gcatttagta gatttgccgg
     4381 gctatggtta tgcaaaagtt tcaaaaacga tgcgttctga ttttgagaaa atgattcaat
     4441 attatttcaa aaatagagaa caattgtatt gtactttttt actgattgat gcctctattt
     4501 cgccacaaaa aatggatatt gaatttgcca attggttagg acaacatcaa gttccatttg
     4561 ttattgtttt tactaaagtg gataaagcta aagctttaga aattaagaaa aatatagata
     4621 agttcaatgg ggaattatta cagacttgga ctacattacc acaatctttt aaaacttctt
     4681 ctgaacggaa aataggccaa cgagaattaa tggattttat ccaattgatt gtaaaagaga
     4741 gcaaaaacca gccatcgaat actaaataaa ctgtccgttg ttttctcgtt ctatatgtct
     4801 gatttttggt aaaataccat atttcttttc gataaattta gctgtttgtt ccgcacaata
     4861 aacactgcga tgttgtccgc cagtacaacc aaaattaatt tgaagatgtc caaaatctct
     4921 atctatataa tcatcaatat taataccaat cgttttccat acaccttcta aaaactcatg
     4981 aacttttgtc ttattttcta aaaaatcaat aaccgcttta tcctttccgt gtaattgttt
     5041 ataaggttca aatctacccg gatttaaaat gccacgacaa tcaaaaacaa agccacctcc
     5101 gttaccatgt tgctcttcag gaatacctct tctataagaa aaactattaa tttcaatttg
     5161 taaaagacta gatttctttt cagtttcttc tggtttaaat agaggactat tcactaattg
     5221 taataaaatg tttttaagct ctggaatatc aatttttagc tcaatatttc ctaaaaacca
     5281 tttgatatta tctaaagctg gaatgatact ttgaagaaag tgcggtcgtt tttctatcaa
     5341 tccacgaaag ccataagcac ccaatgtttg caatattcta atcaatacaa aaccataata
     5401 tttttcttta aattcatttt tatcaagcgc tattaattga cacacttcat tagtataata
     5461 ttctaataat tctgctcgaa ttgggtatgg aatttttgca ccagcttgcc aaatcaaaga
     5521 agcagcatca tattgtaaag cacctttgcg tccaccctga aaatctataa aataaggttg
     5581 gtcatctttt atcattacat tcctagattg acaatcacga aacataaaat ataaatgttt
     5641 ttcttctaat agaaaattac acaaagtatg gaaatcttta ttcaatgcca attcattaaa
     5701 ttgaactctc gccattcgga caaaataata cttaaaagag tttaagtccc aaaacataga
     5761 gcttctatta aattcttgaa ttggaaaaca agtattaaaa tcaaaatttt cagcaccctt
     5821 aatttgtaaa aaagccaatg actttagtgt atttttaaga taattgattg tatgttcgac
     5881 taaaccctct tttttaatga tatcatacaa acttatatcg ccaagatatt cttgtaaata
     5941 ataagcttga ttttctgaaa ttgcataaat ttctgggaca ttaatatgta gttttttaaa
     6001 atgtagtgca tgatgaataa aagtttgatt ttctttgata tcattgcccc aacaaccaat
     6061 tgcttcaatg ttttcagcgg ataatttaaa ataaattcga tcagaaccag cctgtggtaa
     6121 ttgtaatatt aaagtaggtg taattttgta atattgttca aataattgaa ttaattgatg
     6181 ttcgtgtgtt gtcattctat tgcaaatcta tgttttactt tgaaaattga taaaaaaaac
     6241 ttaaaattct cttatcttag cgacaaatta caaaaaaatg gattttaatc aaaccgaaaa
     6301 ccagatttta attacgcaaa cgattagaga tttcgcagaa aaacatatca aaccttatat
     6361 gatggagtgg gatgaaactc aacatttccc agttgaaaca ttaagagctt taggagaatt
     6421 aggtttaatg ggtatcgtaa ttcctgaaga atatggcggt tcaggttttg gttattttga
     6481 atatatcaca gcattaaccg aattgggcaa agtagatggt tctttaacct tatcagttgc
     6541 tgcacataat tctttatgta cgaatcatat ttttaaattt gggaatgatg cacaaagaag
     6601 aaaatggata ccaaaattag catcaggtga gtggattggt tcttggggat taacagaacc
     6661 caatactggt tcagatgcag gaagaatgca atgtgtagct cgacaagatg gagacgattg
     6721 gattataaat ggaactaaaa acttcattac gcacggaatt tcaagtaatg taacagtggt
     6781 tattgcaaga actggagaat tattagatag tcgtggaatg actgcatttg ttgtagaaag
     6841 aggaacacaa ggttttagtg gtggtaagaa agaaaataaa ctaggaatgc gttgttctga
     6901 aacagcggaa atgatttttg acaactgccg tattcccaaa gaaaatgttt taggtaatgt
     6961 tggtgatggt tttgttcaag ctttaaaaat attagatggt ggtagaatat ctattgctgc
     7021 attaagttta ggaatagcaa caggtgctta tgaagcagca gttcaatact caaaagaaag
     7081 agaacaattt ggtaaatcta tttccacatt ccaagcgatt gcgttcaaat tggcagagat
     7141 gcacactaaa attgaagctg caaaattatt aacctatcaa gcagctgata tgtatatgcg
     7201 tggtgagaat atcaataaag aatctgctta cgctaaatac tatgcatcag aagtagctgt
     7261 ttatgcatct actgaggccg tacaaatttt tggtggctat ggttatatta aagattttcc
     7321 agtagaaaaa cattatagag attctaagct ttgtactatt ggagagggta cgtctgaagt
     7381 gcaaaaattg gtaatcagcc gagcaatatt aaaataatct atcttttaga ttactgaata
     7441 attcattttt tttgattata tattatattt gatatacaag acatactttt tgctgtttta
     7501 attgatttct atcaaaatac ttagtaattt tacactaact atgattcttg ctgatgtcaa
     7561 taatatacag aaatattgtc cttctagctt tctgccttat aacaaatttt atatttggtg
     7621 ataatccaga aggtcaagaa tatgataaag aaaaaattaa gaataggtct gcatctattt
     7681 ggagacatga aattaattat ccaccattaa ccacaaaaag tggtttgaga agtcagattt
     7741 ttttccttat tttgtacgat gatggcactt atgagcaaca agtatttatt attcctgaag
     7801 acaaagatgc ttacaaagga attaaatatt atcagctctc tggaaaatgg tcaagcaata
     7861 acaaagaaat atcattagat gaatatggta aaacccagaa aatctctaat aatgatttct
     7921 atactaaatt tgaaaatata gacaacttaa ttgtaaagaa gtaaaaatag ataggtaaga
     7981 aatggaacaa ataaatgttg gaatcattgg agcaacagga tatacaggtg gcgaattgat
     8041 aagagtttta ttacaacatc caaaagtaaa aatagatttt tgttatagtc gttcgaaatt
     8101 tggtaaaaaa atatcggata ttcatcaaga tattttttat tcagacttaa cttttacaaa
     8161 tgagatttat ccaaacgtag attgtgtttt tctatgtttg ccacatggag atactaaacc
     8221 atttttaaaa gagcatcaat tttctgcaag tacaaaaatt atcgatttaa gtaatgattt
     8281 taggttaaaa gctgatgctg gcgaatttgt atatggtttg cctgaattga ataaagaaaa
     8341 aataaaaact gcaaataaaa ttgctaaccc aggttgtttt gccacaacta ttcaattggc
     8401 attattgcct ttggctaaaa atcaattatt gaatggtgat attcatattt cagccatcac
     8461 tggttcaaca ggtgctggaa ataagctcag tgagacatct catttctctt ggagaaataa
     8521 caatatgagt acctacaaaa ctttgacaca tcagcatgtt ggagaaatta atgaaagttt
     8581 gatgcagttg caagcaaatt ataatcaatc tctactttta gttccttatc gtggcgatta
     8641 tccacgtggt attattgcta catcaatcat ttcgacagat gaaagtatag aaaatatcag
     8701 aacgctctat gaatcatttt ataaagatgc tgcttttgtt cgcttatcgg ataccaatat
     8761 caacttaaaa cagattgtaa acactaatta ttgtttgatt tatttggaaa aacaaggcag
     8821 taagctgatt gtagtaagtg caagtgataa cttactgaaa ggtgcatctg ggcaggcagt
     8881 tcaaaatatg aatttgatat ttggtttaga tgaaaaaatg ggcttaaact taaagccaac
     8941 aggtttttaa ctattcatca caaggattga agaacttaca agtattaaag attctttttg
     9001 ttgcaacttc tgaattacct aatttttcaa tataaaaatc aactctagta tttaagttcg
     9061 tatttataga atcaatagga tttgtatctc caaatccaga aacagtgagt ataccacaat
     9121 ctataccttt ggttgttaaa taataagcca caattgctgc tctttgcata ctcagtttca
     9181 tgttctcagt actgttactt tctgtaaaaa catgtgattc tattctaaat tttgaaatat
     9241 tagaattttg ttttaaaaaa tctgcaacat aatcaattat tgggttattg gtatctgaga
     9301 tatcattggc atgtaagtca aaaagaatcg gattgggtaa aatcaattga ttttctgtta
     9361 ctttaaaaat attctgtgct tgaactgatg atgtactcaa taaaaacaaa gaaaaaagtg
     9421 ccatatatgt cgtgatgata tacttcataa agttcgcttt ttttcaaaaa taaataaaaa
     9481 acaataaaac ttgatgtcaa gaaatgttat taaacaaatt tttaggaaat tatgtgttgg
     9541 aatttaactt gcttttttta taaccataga ccaaatagat aatcaaacca atcagcatcc
     9601 acacaataaa tctaagccaa tttaatacac ccaaagttga taataaataa ctgcaagata
     9661 aaaatcctaa gattggaatt gcagaccatt tctttacaaa agtatatcca cttattacaa
     9721 gagcaatgct ccaaaaaatc caaattggaa tatgatgatg ccatacagaa aaaccacctt
     9781 ctaaagaaaa aatagtatca atatatcttt tggcttcatt atttattaag aaaattaaca
     9841 ataaaatgcc ccaaagtatt accatcatta ttcttccgtt gatatagggt actttaaatt
     9901 tcgcttgatt ttctaagcct tcattttttt ctctgtgttg taatattagg ataccaccac
     9961 agaccaatgc aaatgcaaaa agggttccaa tactacataa atctgttact aaatttacat
    10021 ctaagaataa agaaggaata ccaacgataa tgcctgtaat aatagttgca aatcctggcg
    10081 ttttaaattt cggatgaacg gaggctaatt tttttggcaa taaaccatct cgactcatac
    10141 tcatccagat tcgtggttgt ccaatttgaa atactaataa tacactagcg gttgcaatta
    10201 aagcagaaaa tgcaataatt ccgcccatcc aattcattcc aatttgagta aatgcattag
    10261 ctaaaaatgc ttcattatta agctgtgtat aatgtaccat gcctgttaat gccaaactta
    10321 caagaatata caatacagtt gcaattacta aagcataaaa catgcctttt ggcaaatcgc
    10381 gttgtggatt tttacattct tcagctgttg tagaaattgc atcaaaacca atataagcaa
    10441 aaaatactgc cgaaacacct ttaatgacac cattaaaacc attgggagca aatggcgacc
    10501 aattctcagg ctttacaaaa aacaaaccaa tgatgatgat gattaaaatt acagccactt
    10561 taaatacgac cattccattc gcaaagtttt tagattcttt tatacccata tacactaatg
    10621 tcgtaatgaa gaaattgatt aaaaatgcag gtatatttat gatgaaagga ataccaaata
    10681 gttttggtgc agctgcatat agctcaggaa tatatttttt tgcttccaaa aaagaggtgg
    10741 ttagataagc aggcaaatac acaccacaat catgcataaa ttttacaaaa tttccagacc
    10801 aagcaataga aactgtaata tttccaatag catattctaa aattaaatcc cagccaatta
    10861 tccaagcaat taattcgcca aatgtcgtat aagcataagt ataagcactt cctgcaacag
    10921 gaactctcga tgcaaattct gcgtaacaca aagcagaaaa tccacaagca attgctatac
    10981 ccaaaaataa aagactaaca gctggaccac catcaaaaca agcagagcca attgtactga
    11041 aaatacctgc accaacaatt gcagcaatac ctaatgccgc caaatctcta acggtcaaat
    11101 ttttatttaa ctgactttct tgtaattcag gagaactttt aacttcatca agaatatgtt
    11161 gtattgattt ggttctaaaa aagttttgcc atttcatcta acgaatgtat agattttttt
    11221 taagaagttt agaataaatc aaatttctca tctgaagaat tgtgatcatt ttcttcatca
    11281 aaatttgctt ttgaaggtat gattttatat tccgatttat tattcaattc atctaaaact
    11341 ccagcacctc tatctaaatc ataaaaacga ccataattat tttgaaatct taattgtaca
    11401 gaaccaactg gtccatttct atgtttagct acaataattt ccgcagtacc tttcaaagat
    11461 gcaccgtctt tatcttcatc tattttataa tattctggtc tatataaaaa cataaccata
    11521 tctgcatctt gctcaataga acctgattct ctcaaatcgg acaattgtgg ttttttatcg
    11581 ccacctctcg tttccacact tctgtttaat tgagacaatg caattacagg gatatttaat
    11641 tctttagcca aaccttttaa tgaacgtgaa atattactaa tctcttgttc tcgattcatg
    11701 cctttatcac caccaccact cattaattgt aaatagtcga taataaccat tttgataccg
    11761 tgagcatttt gtaatctacg gcattgagct cgaagttcat aaacgttgat ggctggctgg
    11821 tcattgatat aaattggagc ttttgctaaa cgttctactt tatctctcaa atgagcaaat
    11881 tcagcttcag tcatttttcc attagtaacc ttttgagcat caatttctgt ttccatagaa
    11941 attagacgtt ttgctaattg tacaccaccc atttctaaag agaaaattgc aacaggcaaa
    12001 ttataatcaa cggcagcatt acgagcaaca ttcaatacaa atgctgtttt acccattgat
    12061 ggtctagctg caacaatcac taagtctgtt gccttccaac caaaaataaa attatctaaa
    12121 tctttataac cagaaggtac ggaatcttga attacaccac catcattaga aattaattca
    12181 tcgatttgca ttacagattt tgagaccaat tgtccaatac cttgagtagc atttcttaaa
    12241 tttttatcgg taatttcata gattcgtttt tcagcaccat ctaacaaatc gaagacatct
    12301 gtcgtgtctt cataagcatc tttgataata tcattagaga tatgaattaa ttctcgctga
    12361 ataaattttt gaataacaat tcgagcatga aattcgacat ttgcagatga tccaatttta
    12421 ttggttaatt cacttaaata ataagcacca ccaacacttt ctaattgacc ttcttttctt
    12481 aattcttcgc taacagtcaa taagtcgata ggttgagaac gagcaaataa attttgaata
    12541 gctcgatata tttttccatg agcatcaaca taaaaacttt ctggttttaa aatatcagat
    12601 acataactaa tagcgtcttt ttcaatcaat attgcaccca aaacagcttc ttctaaatca
    12661 cgggcttgtg gtggtacttt cccatacaat aaatgagaaa tatcttcatt tttggttctg
    12721 tttgttcttg tttttttaga ggtagtaaat tctgacatag gattgctaaa gtaggtaaaa
    12781 aagtaggctt ctttatatta attaacacac caattctatg ttaataattt tatttgacac
    12841 aaaacactga tatatagcga ggtgttgatt tttatcaaat gaatatttaa tattaaaatt
    12901 aattcgtgca taaaattgtt gatgtcatca aaaaataagt gaataagtta gtggactaag
    12961 gtgtataagt aaagaactta ttaacatgtt tatactatgg ttattctatt ctatttataa
    13021 ttatcaaaaa agtatgtatt ttgtccttgt gaaagaaaag aaagaattaa tacaattaga
    13081 ggttttgaat attacacaag gattttctca atataattct tatagtgtcg tattgggaga
    13141 aaaaaatggc aatagaagac tgccaattgt tattggagca gtagaagcac aatctatttt
    13201 aatagccaaa gaaaatatcc cttctaagcg tccacttacg cacgatttaa ttaaaacgat
    13261 gtttggcaaa tttgatgtaa tgcctttgga agttgttatt aatcatttat tagacggcgt
    13321 gtattatgct attataactg ttgtttatga cggttatgtg attgatattg attctcgcac
    13381 ttctgatgcg attgctatcg ctctaagatg caatatccca atttttattc acaaagatat
    13441 tttagaagat gttgctatta cttttttaaa atcagaagat gaaattattt ctatttctga
    13501 aaatcctgaa gaagaatact tattgcagaa acaatcaagt tttagtcagt attcagtagt
    13561 tgaattggaa agtttattaa aagaagcttt acaaaatgaa gattatgaga aggcagccaa
    13621 aataagagac gagcttaaaa gaaaataaaa agggtttcta tgtttttaaa ctctatctct
    13681 tattctgaac tcaaaatcat aatacaaaat taacattaat aattaagatt atctttgatt
    13741 aatatcaaga aattagcaat ataaagaatt tgaaagtttt aattacagga ataacaggac
    13801 taataggtaa gcatattgct ttcaaattga ataatatnnn nnnnnnnnnn nnnnnnnnnn
    13861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13921 nnnnnnnnnn nnnnnnnagt tctattggtg ttgcatcagg aattgataga aaaaagagca
    13981 ttcctgatga gcaaacgcct ttgataaaaa ctggcgttta ttatgatgat gctaagatag
    14041 atactgaaac attagtaatt gatttttgta ctcaaaataa tctattttat accatcgttc
    14101 gaccaagtgc tgttgttggg ccaggaagtg tttgggtaaa agaacctata gaaagaaaat
    14161 tacataaagg atttttccct cttattaatg atgggagaga gtctgcttgt ttgttagatg
    14221 caagaaattt agctgaaggc atttatagta ttattacaaa agaaattgcc aaaaataata
    14281 tctattattt tatggatgat tttaaagata tttcttggaa aagatatttt acagatttat
    14341 taaaaatggt caatcaaaaa cctagtattt caattcctta ttctgtttta tatcctattg
    14401 ctgttttgat ggaagtaatt gcaaatttta cacataagaa accaatgata gccaaaaaat
    14461 ctatcaatgt tttaggtaca agtagattag tgcatacaca aaaagcaaaa gatgaattgg
    14521 gttataaatc tatttatcaa tatgaagaaa cgatgcagta tattcaagaa tgggtaaaaa
    14581 attactataa aatttaaaac ctaattattt tatctaaaaa aatattgaca ttaatcaata
    14641 aaaattataa ttttatacta atatgaagca taaagctggt tatgtaaata ttttgggcaa
    14701 acctaatgtt ggtaaatcaa ccttaatgaa cgcattaatt ggcgaaaagt tgtctattat
    14761 cacctctaag gcacagacta cacgtcatcg gattttgggt attttgaatg gcgatgattt
    14821 tcaaatggtc atttctgatt tgccaggtat catcaaacct gcttataaga tgcatcaaac
    14881 tatgatgcaa tttgtaaaaa catctttgga agatgctgat gtttttatat atatggtgga
    14941 aataggagac aagccagaaa agcaaccgga agaatacgaa aagattaaac aacttaatgt
    15001 tcccgttata attttattga ataaattaga catcactgac cacgaaatag ttgagtacga
    15061 aagagaactt tgggaggcag attttccaaa agcagaaatc atttcttttt ctgcaaaatt
    15121 caattggaat ttagaaaaat tgatggattt tgccttaaaa catcttccag aatctccacc
    15181 atattttgat aaagatgcga ttacggatag acctgaacgc ttctttgtta ctgaaattat
    15241 ccgagaaaag attttattgt attataaaca agaaattcca tattctacag aagttgtttg
    15301 tacttatttt atggaagatg gtgaaattat caaaattgct gccgaaatat atgttgaacg
    15361 agccagtcaa aagcctattc tgattggcaa aaatggtgaa atgattaaaa aagtaggcat
    15421 tgaagcgaga aaagatttag aagaattttt ccaaaagaaa atctatttag aaacctttgt
    15481 aaaagtagct gatggctggc gaaatagcga caatatgtta cgtcgatttg gttatcgtga
    15541 ttattaatgt agaaacttaa aagcctaaat tatttccttt cagttgagga atagctttat
    15601 tgagaatttc tagttttttt gcaattattg gaagtaaata tttatccgtt ttcttattgt
    15661 tttgttggta ttctaatttg tttcttaaag aaatacaaat tctaatagaa ccatcaatat
    15721 taaagttact ttcttcataa tgattgatat cggataaaaa gtttattgca tcttggaata
    15781 aattttcatc ttgggtttgc attaaaaagt cagatagatt ttcaaaattt tgataggcat
    15841 ttctgtagtt atttagccaa attgctttct tgaaaaaatc taaatgatta accgttgtgt
    15901 cttttaaaat tgacccaata cttcgtagta aagttggatt tttagtatct aaatatggaa
    15961 gagcttcttg gtaggcaatt gcaaaatttt tagtttttac aatgtctaaa gctgctgcta
    16021 tgcattgaag cgaggagtct tttttgatta catattgtgc tactgcaaaa cttaattctg
    16081 aattattttt attcaataaa tttaaagcca tttttctaat atcagctttt gatgattgat
    16141 aaatcaattc tttaagcacg gtatttaata agtttttatc tgtaaaatca ctgggtgaat
    16201 aaatgagaga aagtgcttct ttttgtgtat tccaatctgt atgttgaatg gcttttataa
    16261 aaatatcttg agctgtttta ttgcctttaa tattgtttac aaaactattt aaaacacgag
    16321 agcgtgcgta ataactattg gctctgtcaa attttagtgc ggattcactt tcagttagat
    16381 tttcttttaa ttctgcaggc aatatacctt caggatcaaa atttataaat aatggttttg
    16441 tctttgcagg aaaataaaaa gtagattctt tgttaatgat agacaattct tttcttagta
    16501 tggaatcatt aaaatatata tcaatagtag ttggaatttt aaaaatagga gcagtgtttt
    16561 tgtcttgaat ttgcttgatt tttacttcta tattttttgt ttcagaatga taagtatttt
    16621 gaatatccaa aataggatga cctttatcga aataccattg gttgaaaaac caattccaat
    16681 cctttccagt tacgctttct attactttac gaaaatcact cggctctgca ctgttaaatt
    16741 ggttttgtac taaatataaa tttaaggctt taaaaaatgc atcgtcgcca atataatttc
    16801 gcaataaatg taaaattctt ccaccttttt catagcgaat gccgtcaaac atatctttgt
    16861 gagctttata ataataatct actaaaggtt catttttata ttttgattgt tttaggtatt
    16921 tttcaaaagc atctttgttt actgcatcac cattatcttt gccatactta taatttgccc
    16981 ataaaaattc gccataatct gcaaaacttt catttaaagt caaattagcc caagattctg
    17041 ctgttactaa atcgccaaac caatgatgga ataattcgtg tgcaatgata aaatcgtaat
    17101 tgtcatcaat caaatcttga tgggtcatta ataatttatc atataatatc accgctgatg
    17161 aattttccat tgcacctgcg gtataatcaa aagcaattat ttgagcatat ttatcccaag
    17221 gaaaatctac ttttagtaaa tttgaataaa aagtaagcat ttctggagta ttgccaaaaa
    17281 cttcctgaac atctttataa taatctttaa aagtataata atcgacatca atatttcgcc
    17341 aagtcgtgtg gtattttttg taatctccta ttcctaaaaa aaataaatag ggtgcatgtt
    17401 gtttatcttg tttccaataa tcaatacgct tatttccttt gtttgtgcta cttaccaaaa
    17461 ttccattaga caatgaaata taagccgtat ccagagttac aaaaatctct tgagaacatt
    17521 gttcgtttgg tgcgtctatt gttggaaacc agaatgaatt agcttctact tctccttgtg
    17581 tccacaagtg aaagggtttg tatggatttt tattctcggc attgataaaa tacatacctc
    17641 ttccatcaat ttccaagaca ctttcactta tagaataagg ctttgcaata taatctataa
    17701 gaattgtcaa agtatcagaa gatgtaaatt gtttacctaa ataaatttta agttgtaaag
    17761 aatcgtaatt ataagataga tattgatttt tttgattgtt caataaaatt atatcattga
    17821 tttgcatata ctttgcatcc aatattaatt catcttttgg atagaaatag
//
LOCUS       DAOQCW010000042        17680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1142_length_17680_cov_6.180652, whole genome shotgun sequence.
ACCESSION   DAOQCW010000042 DAOQCW010000000
VERSION     DAOQCW010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 17680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17680
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1142_length_17680_cov_6.180652"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="PLS16_05300"
     CDS             <1..553
                     /locus_tag="PLS16_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNJ60529.1"
                     /translation="DKLLKSYFYFNLITIFVLNLFDMRKKILALTLVTTLFFVACNNS
                     EKPAPEAPAPEATTEEVTEDVAEYDPKRGEGEYDESNLQLAGLDAAMATKGKAISETK
                     CMSCHKATDEKLVGPGWKGVTQRHTAHWIMNFITNPDAMLDKDPELQKQIEICLVRMP
                     DQNLSGEEAREILEFMRQNDGVK"
     gene            complement(621..1115)
                     /locus_tag="PLS16_05305"
     CDS             complement(621..1115)
                     /locus_tag="PLS16_05305"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HNJ60530.1"
                     /translation="MLTKEQEQYRKLVLNPISFKAGLFAKLPLAGVADCRMTELNETT
                     CKITVPYRFLNKNPFDTTYWAVLGMAAEMAGGALLLMYIHKSNPSVSTFVTGLSSKFI
                     KTATGLTTFVCEEGMMIRERVQKAIETGEPDAFNVKSIGYDKHGNITSEFIFEWSIKA
                     RKKK"
     gene            complement(1174..1542)
                     /locus_tag="PLS16_05310"
     CDS             complement(1174..1542)
                     /locus_tag="PLS16_05310"
                     /inference="COORDINATES: protein motif:HMM:NF017935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HNJ60531.1"
                     /translation="MFKKLKAILFAKCPNCYKGNLFLDSNPYHIKNMDKMHKNCPNCN
                     LDYSNETGFYWLSMYTGYFLSGVLSVVFFLIFTSIYGFLNKVGTFVILNTILMLVLIP
                     MIFRWSRSLTFHLAYKMDKS"
     gene            1704..3062
                     /locus_tag="PLS16_05315"
     CDS             1704..3062
                     /locus_tag="PLS16_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60532.1"
                     /translation="MNRIIKDRQNLIFVFAFGLALVVNFFSWDKPFFWDSLLTSSITQ
                     YYFEHGFGNLILPNDLDAGHPPFFYIYIVVFYKLFGLNLFAAHLSMLLPSLLGLFSLV
                     KILQHLNFSYKLQAFTILGYFFIPAILTQHTLVSYDLMLSSLYLFGIYCILFERKILL
                     SIIAIGICSVAARGLFFIFSLFFVYYLNNKNLKKSILIFLPALLLFGLWMFYHTLFTQ
                     HFIALENNWSEHRKLVNFRGLTMNFIALARVLFDFGIVLLFIIQLLQFYITKNIGRLQ
                     LFWIIPFVVFAGGFVFLSNPIGHRYFLVVYIFLLIDSIYFLNKQYKNWLWILCLLPLG
                     HFQIYGNRISNGWDCTLEYLSFIFLKNDFDRYIKSENIDYSKLGTVFPINISKQQYYL
                     SGDSRSMVNIHGKIIDDYSFILYSNVGNDFSDEQLNRMQQYKIEKIEKHGKVTMILYY
                     TR"
     gene            3125..4729
                     /locus_tag="PLS16_05320"
     CDS             3125..4729
                     /locus_tag="PLS16_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60533.1"
                     /translation="METLKPKYNKQDIINVGLILLLGFLIARQLQDYMDVLLWDEAIY
                     LDRGFLLWKIIPNTWGPVYSVWYKLLSYIEQNKIALYYLNFKILFLLSSILAYLFMVA
                     LRMPKVMALFFTCMWLIHPYNLPSWPKISHFVICIILFAAIFIQYLKSTINQIVVFSI
                     AFLICGFARPELYLSFIFSVLLLFILIFINRKNINPSNILSICVWLLFVGITYKLYRT
                     PLTSGDAQRGLGVFIQHFALNYSIWNKQDILWWFDFQDAVMRCFKPPYTLKNFVQNPY
                     LWQHFSFNIQLLLSKSFSLISNLIFPFHFISKKIFFVLSSLLSLYLLYKNKCQIKYFF
                     QLIKEQFLLFGFLLISVLPSIASSIYAHPRTHYLVILFPFIFFIFSAFFPKHKTSNVF
                     HSLLIFVVFIFFMPKAKDFQFFDLYGTDKNMMTKKTINFIEDKYQDKPTTILDIDGLM
                     PSLMNSNFKSSNIRPLIKEDSVLISSIVLKEQPDIIYVTPTMMKLKKNKTDSLFQQMI
                     AEPERFNYYKQAIPTLGDVYLLEKTK"
     gene            4797..6410
                     /locus_tag="PLS16_05325"
     CDS             4797..6410
                     /locus_tag="PLS16_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60534.1"
                     /translation="MLDKFKRELFIINDFIAICLLIIMGIFVVHNLENNMDVLLWDEA
                     RYLDRGFWLWKAFPKTWGPIYSVWYKILSYVESNRIALYYLNYKVLFIASTILTFSFM
                     RAYKASLFVAFFLSSMWLVNTYNLPAWPKISHFSICIILSAGIIVAFLKNTINKTIVF
                     AIAFLICGYARPELYLSFIFILLLLFVLSIKNIRTLNKSNVLYFALLFFLILLSYKAY
                     RTPLNNEDASRGIGVFLQHYAYNYYTWTKQDVIWWFDVQEALDKSFKAPYQLKEIMLN
                     VNSPFWKHILYNTKLFFSHLVFIISNLFFPFAHLNKIPFFIGTCLIGLYVFWCFRQSL
                     PLIIERFKTNWLVFLVLILGILPSIASSIYAHPRTHYLVLLMPLIFLFLSTVFKESLN
                     SSWKGLVAFALFFILTSPKAKDFKYFDLFGTDKNMMNKKAILFLENKYKTDSVNVFDI
                     DGQLNTLMTQNFKSYNIGAFISKPDYLLSQFIQTERPNVVYVTPTMFKLKRTQKDSIF
                     QQMIHQPESFGYQRNSIPGLTDTYFLVIK"
     gene            6525..7856
                     /locus_tag="PLS16_05330"
     CDS             6525..7856
                     /locus_tag="PLS16_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60535.1"
                     /translation="MGTGKTFLDTVLYTRDQEGYEKKVVFLFIYSLFCNSNTFSQFSA
                     VSSIGGIFVVILIPLMLLLTGVYWYVAIFVYKRLSLIDLFVLIWCLTPIYNAIASHLT
                     VDVKILKALINAMPRYLVVSASLMYFLIKSEKITIKQYCLAGVMVGWFDLILYVILNI
                     YLDPQTYKADESLVGYNPSKGGYIWRFGSVYIIFALSYHFVVFLVRNKFLHLIATMIF
                     LAYMFFLDKQRTEILSVLIPIIIYMFFKLRWYEVINRLSQLVLFSGLILGIIYCINPM
                     LLQFTADMFINFFKFMMGMETGEASADSRIIQFTNAYDFFMRHPTFMFWGIGYVKPET
                     LYLEMGKFIYSDTGIVGILVAHGIIGTILQTSMFLYPIYVFFKVKHYRNDIYYNMGML
                     GCVVGFTSGMFSGGYALNAFGIFYFFAFVEYFRVKEKIYWKHKRLEREKAT"
     gene            7840..9348
                     /locus_tag="PLS16_05335"
     CDS             7840..9348
                     /locus_tag="PLS16_05335"
                     /inference="COORDINATES: protein motif:HMM:NF023218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNJ60536.1"
                     /translation="MKKLLKYFLIIFVIFAILKNCTPSKKEKKQIAEVTTKSSSIDIG
                     LKLDYSKEQQLYADNLFGLNTGFIWTKEIDKSKDFVELLKESGNVVLRFPGGGVANNY
                     YPDEVGYGYNPKKRPIGFKSIMLYNQTKDRKFNIIENFVSLCKQTNAKAIYCANVLDG
                     SVEKMQIVIERLKRENIELIGVELGNEFNMGNYRHVFPNGQIYLDTIQKYIQAIRKNY
                     PDLKIGLVAEMLDVNKLKDKRSEFMHEWNKKISTADFDAYIVHSYFPIDDKQKNFDEI
                     FLKSVDISNPYYTNYTTKLLEYFNSIDKEQKKKIWITEWGIFNLYGGNTFLQAASTAQ
                     FLNNIIQYNKLNRIDIACLHDIGAMIGQAHPKRQYTYQKDNFAVGALYFPYKFLADII
                     VKNKAKLIPHQLDKEVASNFVVQTFYSSENKKTYLYFINTAPITVHLAIPPTKLKKQI
                     EYIVADKLYAHTGFAAFLKDYPSKNTLIQYKKENRSTNKIKIYPYSLGVISF"
     gene            complement(9472..11196)
                     /locus_tag="PLS16_05340"
     CDS             complement(9472..11196)
                     /locus_tag="PLS16_05340"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNJ60537.1"
                     /translation="MIFYKQLLRNILKVRLFVILSLFFFNSLNAQSTILIPNGQFQID
                     YSKQLIVSNFNILAINSTLEEAFNKIQLDKIYTFSDPISSVEIGKSYPIKDEEGHRYQ
                     LFFTELPLIHITTNNIIVDEPRRFTSFFMAESNGMIVNEHIGIEYRGASSQNYPKKSL
                     RIEFWADSTGEETKDVRLLGMREDDDWNLQAMYNEPLRFNNVVSFDLWQQIDTLYYIK
                     KEQKAVNGVNMKYAEVFINNEYRGVYGVGERVDRSQLKLKKFKDDEIRGELYKGSDWT
                     DAVLMNKAPVYDNSKALWAGFEYEYPDAEEIIDWENLYNLISFVEKSSDEEFYLAYPD
                     KMVVDNLVNYFIFLNLTYAADNTGKNLYIAKYDKNEPYFYVPWDLDGTFGLFWDGSNH
                     NNTTGLLSNNLYDRLLKDCSENGFVERLKSRWKFLRTQVITYENITQMFEKQMEYLSR
                     NGIYQREELAWNKYIYPKTQYAYTTNWLKLRLNNLDSFFSQDCKGFDFKDNIPYSFIL
                     FPNPTSSLLNIQSEISDEFYIYDINGIVLKSGYISTGLNIIDVSDLKCGIYFFKPLKS
                     NPNKFIVQ"
     gene            complement(11244..12269)
                     /gene="galE"
                     /locus_tag="PLS16_05345"
     CDS             complement(11244..12269)
                     /gene="galE"
                     /locus_tag="PLS16_05345"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNJ60538.1"
                     /translation="MKVIVTGGTGYIGSHTIVDLLENDFEVVCIDNLARSTKYPIGAI
                     EQITEKKFKFYQIDLCNKQETFDVLEQHKDAVGIIHFAAFKTVPESVANPILYYNNNL
                     ISLNNILEGIQKFGIPNFVFSSSCSVYGNAKALPVTEETPFQEAESAYAHTKQIGEQI
                     IQHYSKVNTTKSILLRYFNPVGAHPSGILGEMLLNRPDNLVPYITQTAIGKLEQLTVF
                     GGDYDTPDGSCVRDYIHVCDIAHAHTLALKYLIENQNHSNCEIFNLGTGNGVSVLEAI
                     HAFEKTTGQKLNYKVGARRAGDVEKIYANNEKASKALKWHCQYNIEDAMLSAWKWENK
                     MKELALL"
     gene            complement(12274..13134)
                     /gene="rfbA"
                     /locus_tag="PLS16_05350"
     CDS             complement(12274..13134)
                     /gene="rfbA"
                     /locus_tag="PLS16_05350"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNJ60539.1"
                     /translation="MKGIILAGGSGTRLYPITMAISKQIMPIYDKPMIYYPLSVLMQA
                     GINEILIISTPHDLPHFERLLGDGKKYGCNFQYAIQEVPNGLAQAFVIGKSFIGDDKV
                     ALILGDNIFYGSGLEDLLVKNNNPDGGVIYAYHVSDPERYGVVEFDEQLNAVSIEEKP
                     TNPKSNYAVPGLYFYDNDVVEIAENLEPSPRGEYEITDVNKEYLKRGKLKVGILDRGT
                     AWLDTGTFNSLMQASQFVQVIEERQGLKVGCIEEIAYKKGFITADQLKEIAQPLLKSG
                     YGQYLIELVK"
     gene            13270..13962
                     /locus_tag="PLS16_05355"
     CDS             13270..13962
                     /locus_tag="PLS16_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ60540.1"
                     /translation="MSTGEKVFKVLVVDDEQDILEFLSYNLEKDGFLVSTAENGKVAL
                     EKAKKEEPDIILLDVMMPEMDGIEACRTLREMPQFENTIIAFLTARTEDYSQIAGFET
                     GADDYISKPIKPRVLISRLRALLRRYETKESKASFLEVGDIKIDRERYLITFKGTEMA
                     VPRKEFELLYLLASKPGKVFKRDEILNEIWGRDIIVGDRTIDVHIRKLREKLGEDLIK
                     TVKGIGYKFDEI"
     gene            13959..15008
                     /locus_tag="PLS16_05360"
     CDS             13959..15008
                     /locus_tag="PLS16_05360"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ60541.1"
                     /translation="MRGNSSLNTISIYTSIAVAFFSTAFFFVVNELFEHQINIFAHFI
                     FFFAIFFCSFILFRYVLKQLIYDKIKIIYKTIGKPLKFQHEVKSSKGNIMLTVERDVA
                     EWAINKNKQIRELRKLEQYRKEFVGNVSHELKTPIFNAQGYIETLLDSNLEDKDFMKS
                     YLGKAASNIDRLEVIVSDLLEISKFETGRIQLEKDGFDIVKLIKKVIAQYQFLAEDCK
                     VKVKVFNHENEFFVFADEKRIMQVLENIISNSIKYNNEKGKVDIRIYDLDEQYMIEIS
                     DNGPGIAEEHLPRLFERFYRADKSRARKVGGTGLGLSIVKNIIEAHEETINVSSTLNE
                     GTMFSFTLHKYRERL"
     gene            complement(15003..16187)
                     /locus_tag="PLS16_05365"
     CDS             complement(15003..16187)
                     /locus_tag="PLS16_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNJ60542.1"
                     /translation="MLSQRQLFLQHVAQTSPEPLALEIEKAEGIYLYDISGKKYIDLI
                     AGISVSNIGHCHPNVVNAIQQQAQTYMHTLVYGEFIQSPQVQLAKYLSDLLPENLNSI
                     YFTNSGAEATEGAMKLAKRFTGKPEIISCVNAYHGSTQGALSLLGDEFFKSAFRPLLP
                     DTRQIRYNNLEDIELITERTAAIFLESVQAEAGVIAPTKEFLIAVRQKCNETGTLLVL
                     DEIQTGCGRTGKLFAFEHFGILPDIILLAKGLGGGMPIGCFISDKKILQSLTHNPVLG
                     HITTFGGNAVCCAAALANIQTIHQQELYIDVPKKAALFLQLLKHPKIKKINNFGLLMA
                     VYLDSFEQIHQVIQYCLKNGLITDWFLFANNCLRIAPPLIITEDEIQASCSILLAALD
                     TL"
     gene            complement(16284..17057)
                     /locus_tag="PLS16_05370"
     CDS             complement(16284..17057)
                     /locus_tag="PLS16_05370"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNJ60543.1"
                     /translation="MQKTLLLILALLLCSGLAIAKGKKTKNIKSTSKLETVDKTIKEE
                     IITLDTIVEKMEPKISTPKTTYVVIETTVGNIKIKLFDETPLHKANFIKIVQDGVLDS
                     TLFHRVIPEFMIQGGDPLSKNAVPGQRLGTGGLDYRVPAELFPNIIHQKGALAAARDG
                     NPQKASSSTQFYIVVGKTYTDNELNILGSRTGNSWTEAQKEIYKTIGGTPMLDMQYTV
                     FGQTVEGQDIVDKIVSAPRDPYDRPFNDIRMLKVSVIEE"
     gene            complement(17083..17439)
                     /locus_tag="PLS16_05375"
     CDS             complement(17083..17439)
                     /locus_tag="PLS16_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60544.1"
                     /translation="MSEIANSEALDFSIEDWKKVTDFFKNRFDKIPDLTNMLFIIGHR
                     EIGQNLVKLSKEQKQDVIHVGVCALLSLADYYTFKGIDEDGWPHYEYNRNKEKLTHEQ
                     QEMLLKKMIIQYIKKL"
     gene            complement(17510..17668)
                     /locus_tag="PLS16_05380"
     CDS             complement(17510..17668)
                     /locus_tag="PLS16_05380"
                     /inference="COORDINATES: protein motif:HMM:NF021915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60545.1"
                     /translation="MQKYVEDPLAEEILQNKIQNGDTILVDTDKEDKEKLTFSVKSKK
                     KKQIKEEK"
BASE COUNT         5933 a         2518 c         2652 g         6577 t
ORIGIN      
        1 tgataaatta ttaaaatcat atttctattt caatttaatt actatctttg ttttaaattt
       61 atttgatatg agaaaaaaaa tattagccct tactttagta acaacattgt tctttgttgc
      121 ttgtaataat agtgaaaaac cagctcctga agcaccagct cctgaagcga ctacagaaga
      181 agtaacagaa gatgttgcag aatatgatcc taaacgtgga gaaggagaat atgatgaatc
      241 aaatctacaa ttagcaggat tagatgctgc aatggctaca aaaggaaaag caatttctga
      301 aacaaaatgt atgtcttgcc acaaagcaac ggatgaaaaa ttagttggtc caggttggaa
      361 aggtgttact caaagacata cagctcattg gattatgaat tttattacaa atccagatgc
      421 aatgttagat aaagaccctg agttacaaaa acaaattgaa atttgtttag ttcgtatgcc
      481 agaccaaaat ttaagtggtg aagaagcgag agaaatatta gaatttatgc gtcaaaatga
      541 tggtgtaaaa taaatattgc aacaaattat tataaaaaaa ggtcagaaaa aattctgacc
      601 ttttttattt gtaatttttt ttacttcttt tttcttgcct taatactcca ttcaaaaata
      661 aattctgatg taatatttcc gtgtttgtca tacccaatag atttcacatt aaatgcgtct
      721 ggttctccag tttctattgc tttttgaaca cgttctctaa tcatcatgcc ttcttcgcaa
      781 acaaatgtag ttaaaccagt tgctgtctta ataaatttgg aagaaagtcc agtaacaaat
      841 gtagaaacag aaggattact tttgtgaata tacattaata ataaagcccc accagccatt
      901 tcagcagcca taccaagtac agcccaatat gttgtatcaa aagggttctt atttaaaaaa
      961 cgataaggga cagtaatttt acaggttgtt tcatttaatt cagtcatgcg acaatcagca
     1021 actcctgcta aaggaagttt tgcaaaaagt ccagctttaa aagaaattgg attaagcacc
     1081 aattttctat attgttcttg ttctttggta agcatcttta atatttattt tgatgaagat
     1141 aattgatatt cacgatatta ttataaatat aggttagctt ttatccattt tataagctaa
     1201 gtgaaaagtt aggcttctag accatctgaa aatcattgga ataagcacta acattaaaat
     1261 agtatttaaa ataacaaaag tgccaacttt gtttaaaaat ccataaatgg aagtaaagat
     1321 tagaaaaaaa acaacagata aaacaccact taggaaataa ccagtgtaca tagatagcca
     1381 ataaaaacca gtttcgttgc tataatctaa gttgcaattc ggacaatttt tatgcatttt
     1441 atccatattt ttaatatgat atggattaga atctaaaaat aaatttcctt tataacaatt
     1501 aggacatttt gcaaaaagaa tagcttttaa ttttttaaac attacactta tttatcttac
     1561 aaaagtaaga tgttttaata atatttttaa atcttgacaa tagagatgta ttttaatagt
     1621 acaaacgata taatcaggat acttttacaa agaatttcca tctttttctt ctaatattta
     1681 tcactacttt tgtttggtaa cagatgaaca gaattattaa agatagacaa aatttaattt
     1741 ttgtatttgc ttttggattg gcattagttg ttaatttttt ttcgtgggat aaaccctttt
     1801 tttgggatag cttactaact tcttctataa cccaatacta ttttgaacat gggtttggta
     1861 acttaatttt acctaacgat ttagatgctg gacatcctcc ctttttttat atttatattg
     1921 ttgtttttta taaattattt ggcttaaact tatttgccgc acacttgtca atgctacttc
     1981 caagcttatt aggcttgttt tcattagtaa aaattttaca acatctaaat ttctcctata
     2041 aattacaagc atttaccatt ttgggctatt tttttattcc tgcaatactc acacaacata
     2101 ctttagtaag ctatgattta atgctttcct cgctttattt atttggtatc tattgtattt
     2161 tatttgaacg aaaaatttta ttgagtataa ttgctatagg tatctgtagt gttgcagctc
     2221 gtggattatt ctttattttt agtttgtttt ttgtttatta tttaaacaat aaaaatctaa
     2281 agaaatctat actaattttt ttaccagcat tgttattatt tggcttatgg atgttttatc
     2341 atactttatt tactcagcat tttattgctt tagaaaataa ttggagcgaa catcgaaagt
     2401 tggttaattt cagaggatta actatgaatt ttattgcctt agcaagagtt ctttttgact
     2461 ttgggattgt attacttttt attattcagt tgttacaatt ttatataaca aagaatatag
     2521 gtaggcttca attgttttgg attattcctt ttgtagtttt cgctggaggt tttgtttttt
     2581 tatctaaccc aattggacat cgctatttct tggtcgtgta tatatttctc cttattgatt
     2641 ctatttattt tttaaataag caatataaaa attggctatg gatattgtgt ttgttgccgt
     2701 taggtcattt tcaaatatat ggtaatcgca tttcaaatgg ttgggattgt actttagaat
     2761 atttgtcatt tattttctta aaaaatgatt ttgacagata tataaaatca gagaacattg
     2821 attattcaaa attaggtaca gtttttccca taaatatatc caagcaacaa tattatttat
     2881 caggtgatag tcgttcaatg gttaatatac atggaaaaat cattgatgat tattcattta
     2941 ttttatattc aaatgttggg aatgattttt cagatgaaca actaaatcga atgcaacaat
     3001 ataagattga aaaaatagaa aaacatggaa aagttaccat gatactatat tatactaggt
     3061 gatttatcag ctaaacaatc tcattccaac tcataaaaaa acagtatctt agtttccatt
     3121 tttaatggaa accttaaagc caaaatataa taaacaagat attattaatg taggtctaat
     3181 cctattgctt gggtttttaa ttgctagaca gcttcaagat tatatggatg tactgctttg
     3241 ggatgaagca atatatttag atagaggatt tttattatgg aaaattattc caaatacttg
     3301 gggacctgtt tattcagttt ggtataaatt attatcctat attgagcaaa ataagatagc
     3361 tttatattat ttgaatttca aaatactgtt tcttttaagt tcaattttgg cttatctttt
     3421 tatggttgct ttgagaatgc caaaagtgat ggctttattc tttacttgta tgtggctgat
     3481 tcatccttat aatttgccgt cttggccaaa aatatctcat tttgttattt gtataattct
     3541 atttgctgct atatttatac aatatttaaa aagtaccatc aatcaaattg ttgttttttc
     3601 aattgctttt ttaatctgtg gctttgctcg tcctgaacta tatttatcgt ttattttttc
     3661 tgttttattg ttatttattt taatttttat caatagaaag aatattaacc catcgaatat
     3721 cttaagtatt tgtgtttggt tgttatttgt gggtattact tacaaacttt accgaacgcc
     3781 actaactagt ggtgatgctc aaaggggttt gggtgtattt atacaacatt ttgcattaaa
     3841 ttattctatt tggaacaaac aagatatttt gtggtggttc gattttcaag atgcagtaat
     3901 gcgttgtttt aaacctcctt atacacttaa aaattttgta caaaatcctt atttgtggca
     3961 acatttttcg tttaatattc aattattatt gtcaaaatca tttagcttaa taagtaatct
     4021 aatttttcca tttcatttta ttagtaaaaa aatatttttt gtacttagtt ctttattgtc
     4081 gctttatctt ctatataaaa acaagtgtca aattaagtat ttctttcaat tgataaagga
     4141 acagtttctt ttgtttggct tcttgttaat atctgttttg ccatctattg catcatctat
     4201 ttatgcacac cctagaacac attatttggt tattttattt ccttttatct tttttatatt
     4261 cagtgcattt tttccaaagc ataaaacatc aaatgttttt cacagcctac tcatctttgt
     4321 agtttttata ttttttatgc ctaaagcaaa agactttcag ttttttgatt tatatggtac
     4381 agataaaaat atgatgacca aaaaaacaat taattttatt gaagataaat atcaagataa
     4441 gccaactaca attttggata ttgatggatt gatgccatct ttgatgaata gtaattttaa
     4501 atcaagtaat attcgacctt taataaaaga agattctgta ttgatttcat cgattgtact
     4561 aaaagaacaa cccgatatta tttatgtcac gccaaccatg atgaaactaa agaaaaacaa
     4621 aacagatagt ttgtttcaac aaatgatagc agaaccagaa cgatttaatt attataaaca
     4681 agctattccg acattaggag atgtttatct attggagaaa acgaagtaaa taaaaatata
     4741 attaaaccca ttgatagtta taaactttaa aaacaactta ccttgtgaaa gattttatgc
     4801 tagataaatt taaaagagaa ctttttataa ttaatgattt tatagctatt tgtctattaa
     4861 taataatggg catatttgtg gtgcataatt tagaaaacaa tatggatgtt ttgctttggg
     4921 acgaggcaag atatttagac agaggatttt ggctttggaa agcatttcca aaaacttggg
     4981 gaccaatcta ttctgtatgg tataaaatcc tttcctatgt tgaaagtaat agaatagctt
     5041 tgtattatct gaattacaaa gtacttttta ttgcaagtac aattttgact tttagtttta
     5101 tgcgagcgta taaagcatct ttattcgtag cttttttctt gagttctatg tggctagtaa
     5161 atacttataa tttaccagca tggcccaaaa tctctcattt ttcaatatgt atcattttat
     5221 ctgcgggaat tattgttgca tttttaaaaa acactatcaa taaaactatt gtttttgcaa
     5281 ttgccttttt gatttgtggg tacgcacgtc cagaattata cttgtcattt atctttatac
     5341 ttctcttgct ttttgtatta agtattaaaa atatcagaac attgaataaa agtaatgttc
     5401 tttattttgc tttgctgttt tttctaatac tgttaagtta taaagcctat cgaacgccgt
     5461 taaataatga agatgcatca agaggaatag gtgttttttt gcagcattat gcatataatt
     5521 attatacttg gacaaaacaa gatgtgattt ggtggtttga tgttcaagaa gctttggata
     5581 aaagttttaa agctccttat caactgaaag aaatcatgtt gaatgtaaat agtccatttt
     5641 ggaagcatat tttgtataat acaaaattat tttttagtca tttggttttc atcatttcaa
     5701 atctattttt tccttttgct catcttaaca aaattccctt ttttattggt acatgtttga
     5761 ttggtttgta tgttttttgg tgttttagac aaagtttgcc tttaattatt gaaagattta
     5821 aaacaaattg gcttgtattt ctagttttaa tcttagggat tctgccgtcg atagcatcaa
     5881 gtatatatgc tcacccaaga acacattatt tagtattgct aatgccttta atttttctat
     5941 ttttaagtac cgtttttaag gaaagcttaa attcatcatg gaagggatta gtagcatttg
     6001 ctttattttt tatacttact tctccaaaag ctaaagactt taagtatttt gatttatttg
     6061 gtacagataa aaatatgatg aataaaaaag caattctttt tttagaaaat aagtataaaa
     6121 cagattctgt taatgttttt gatatagacg gacaactcaa cactttaatg acacaaaatt
     6181 ttaaatcata taatataggt gcatttattt caaaaccaga ttatctctta tctcaattta
     6241 ttcaaactga acgtcctaat gttgtttatg ttacgccaac gatgtttaaa ttaaaaagaa
     6301 cacaaaaaga ttcaattttt caacaaatga ttcatcagcc tgaaagtttt ggataccaaa
     6361 gaaatagtat tccaggattg acagacactt attttctagt aataaagtag aagcgagttt
     6421 ttaaaatgcg ttccttagaa aattctgtta aataccaata ttctgaaaat ttctacaata
     6481 aaaactacta cctttggcat ttagtcgaat aaattgcata acgtttggga acaggaaaaa
     6541 catttttaga tacggtgctt tatactcgtg accaagaagg ttatgagaaa aaagtagttt
     6601 ttctttttat ctattcttta ttttgtaaca gtaatacttt ttctcagttt tcagctgtga
     6661 gtagtattgg aggaattttt gtagtaattc ttattcctct aatgttatta ttgactggag
     6721 tctattggta tgtagctata tttgtatata agcgtttatc attgattgat ttgtttgtac
     6781 ttatttggtg tttaacgcca atttataacg ctattgcctc acatcttacc gttgatgtta
     6841 aaattttgaa agccttaatc aatgcaatgc caagatacct agttgtatct gcaagtctga
     6901 tgtatttttt aatcaaaagt gagaaaatta caataaaaca atactgcctt gctggcgtta
     6961 tggtgggttg gtttgattta attttgtatg tcattctaaa tatctattta gacccacaaa
     7021 cctacaaagc agatgaaagt ttagtgggat ataatccatc aaaaggtgga tatatttggc
     7081 gttttggtag tgtttatatc atatttgcct tgtcctatca ttttgtagta tttttagtaa
     7141 gaaataagtt tttacattta atagccacca tgattttctt ggcttatatg tttttcttgg
     7201 ataaacaacg tacagaaatt ttatcggtat tgataccaat aattatttat atgtttttca
     7261 agttgagatg gtatgaagtc attaaccgat tatcacagtt ggtcttgttt tctggtctta
     7321 ttttggggat tatttattgt atcaatccta tgcttctgca atttacggca gatatgttta
     7381 ttaatttctt taagtttatg atgggtatgg aaactggaga agcatctgca gattcaagaa
     7441 ttattcaatt tacaaatgct tatgatttct ttatgcgtca tcccactttt atgttttggg
     7501 gaattggata tgtgaaacct gaaacactat atctagaaat gggaaaattc atttattcag
     7561 atactggaat tgttggtatt ttagtagcac atggaattat cggtacgata ttacaaactt
     7621 ccatgttctt atatccaatt tatgtatttt ttaaagtaaa gcattatcga aatgatattt
     7681 attataatat gggaatgttg ggttgtgtgg ttggttttac cagtggtatg tttagtggag
     7741 gttatgcttt aaatgcattt ggtattttct atttctttgc ttttgttgaa tattttagag
     7801 taaaagaaaa gatttattgg aaacataaac gcttggaacg tgaaaaagct acttaaatat
     7861 tttttgataa tatttgtcat ttttgcgatt cttaaaaatt gtacgccatc taaaaaggaa
     7921 aaaaaacaaa ttgcagaagt tacaaccaag tcttcctcta ttgatattgg tttaaaatta
     7981 gattattcaa aagagcaaca attatatgcg gataatctat ttggtttaaa tacaggattt
     8041 atttggacaa aagaaattga taaatcaaag gactttgttg aattattaaa agaaagtggc
     8101 aatgttgttt tgagatttcc aggaggaggt gtagccaata attattatcc agatgaagtt
     8161 ggatatggct ataatccaaa gaaaagacca ataggattta aaagtatcat gctctataat
     8221 caaacaaaag atagaaaatt taatattata gaaaattttg taagtctatg taaacaaacc
     8281 aatgcaaaag caatttattg tgccaatgtt ttagatggaa gtgttgagaa aatgcaaatc
     8341 gttattgaac gattgaaaag ggaaaatatt gagctaattg gtgtagaatt aggcaatgaa
     8401 tttaatatgg gaaattatcg acatgttttt ccaaacggac aaatttattt agataccatt
     8461 caaaaataca ttcaagcaat aagaaaaaac tatcctgatt taaaaatagg attagttgct
     8521 gaaatgctag atgtcaataa attaaaggat aagagaagtg aatttatgca tgaatggaat
     8581 aaaaaaattt ctaccgcaga ctttgatgct tacatcgttc attcttattt tccaatagat
     8641 gataaacaaa agaattttga tgaaattttc ttaaagtctg ttgatataag taatccatat
     8701 tatacaaact atacaacaaa attattggaa tattttaact caattgataa agagcagaag
     8761 aaaaaaattt ggattacaga atggggaatt tttaatttat atggaggtaa tacattttta
     8821 caagctgcat cgactgccca atttttaaat aatataatac aatataacaa gcttaatcgt
     8881 attgatattg cttgtttaca cgatattgga gcaatgatag gacaggctca tccaaaacgt
     8941 caatatactt accaaaaaga taattttgct gttggagcgt tatactttcc ttataagttt
     9001 ctagctgata ttattgtaaa aaataaagcc aaattaatac cacatcaatt agataaagaa
     9061 gttgcctcta atttcgttgt acaaacattt tatagctcag agaataaaaa aacatacctt
     9121 tattttatca atactgctcc aataacggtt catttagcta ttccacctac aaaattaaaa
     9181 aagcaaattg aatatattgt tgcggataag ttgtatgcac atactggatt tgctgcattt
     9241 ttgaaagatt atccttcaaa aaacacctta attcaataca aaaaggaaaa cagatctact
     9301 aataaaatta aaatttatcc ttattcactt ggtgttatca gtttttagaa aggaattata
     9361 agttcaattc tttcatttta ttttcccatt tccaagcgga aagcatagcg atttttacat
     9421 aggcttatta tttaagacaa ttcttattag aagggttttt ctaatcaaag cctattgtac
     9481 aataaactta ttaggattac tttttaatgg tttaaaaaaa tatattccac atttcaaatc
     9541 tgaaacatct attatattta gccccgtaga tatatagcca cttttaagaa caataccatt
     9601 gatatcataa atatagaatt catctgaaat ctcagattga atatttaata aagatgatgt
     9661 tgggtttggg aatagtataa atgaatatgg aatattatct ttaaaatcaa atcctttaca
     9721 atcttgacta aaaaaactat ctaagttatt cagtcttaat ttaagccagt ttgtagtata
     9781 agcatattga gttttggggt aaatatattt attccaagct aattcttctc tctgataaat
     9841 accatttctt gataagtatt ccatttgctt ctcaaacatt tgtgtaatgt tttcataagt
     9901 aattacctga gttctcaaga atttccatct actttttaat ctttctacaa atccgttttc
     9961 tgaacaatct ttcaataatc tatcgtataa attatttgac aataatcctg tagtattatt
    10021 atgatttgaa ccatcccaaa ataatccaaa ggtgccgtct aaatcccaag gaacataaaa
    10081 ataaggttca tttttatcat attttgcaat gtatagattc ttgccagtat tgtctgctgc
    10141 ataggttaaa ttaaggaata taaaataatt taccaaattg tccactacca tcttatctgg
    10201 ataagctaaa taaaattctt catcagaaga cttctctaca aaagaaatta aattatataa
    10261 attctcccaa tctattattt cttctgcgtc tgggtattca tattcaaatc cagcccataa
    10321 tgctttagaa ttatcataaa caggtgcctt attcattaag acagcatcgg tccaatcgct
    10381 tcctttatat agttctcctc ttatttcatc atccttaaat tttttcaatt tcaattgaga
    10441 tctatcaact cgttcgccta caccataaac gcctctatat tcattattaa taaacacttc
    10501 tgcgtatttc atatttacgc cattaacagc tttctgttct tttttaatat aataaagcgt
    10561 atctatttgt tgccataaat caaacgaaac tacattgttg aatcttagtg gttcattata
    10621 cattgcttgt aaattccaat catcgtcttc tctcatgccc aataaacgaa catctttagt
    10681 ttcttcgcca gtggagtctg cccaaaattc aattctcaaa gatttttttg gataattttg
    10741 tgagctagca cctctatatt ctattcctat atgctcattt acaatcatgc catttgactc
    10801 tgccataaaa aatgatgtaa aacgccttgg ctcatctact ataatattat ttgtagtaat
    10861 atgaataagc ggcaattctg taaagaataa ttgatatcga tgtccttctt catcctttat
    10921 tggatatgac tttccaatct ctacactact tattggatct gaaaaagtat agattttgtc
    10981 tagttgaatc ttattgaatg cttcttctaa tgttgaattt attgctaaaa tattaaaatt
    11041 agaaacaatt aattgtttag aatagtctat ttggaattgt ccattaggaa ttaatattgt
    11101 actctgtgca tttagagaat tgaaaaaaaa taacgataga atgacgaaaa gtctaacctt
    11161 taatatattc ctcagtaatt gtttataaaa aatcatatca aatcaaagat ataacaaaat
    11221 cagctaagaa attaaatcaa aacttacaac aatgctaatt ctttcatttt attttcccat
    11281 ttccaagcgg aaagcatagc atcttcaata ttatattgac aatgccattt taaagcctta
    11341 gatgcttttt cattgttggc ataaattttt tctacgtcgc cagcacgtct tgcaccaacc
    11401 ttataattta atttttgacc tgttgttttt tcaaatgcat gaatggcttc taaaacagaa
    11461 actccatttc cagttcctaa attaaaaatc tcacaattag aatggttttg attttcgatt
    11521 agatatttta aagctaaagt atgtgcatgt gcaatatcac aaacatgaat ataatcacgc
    11581 acgcaagaac catctggggt atcataatca ccaccaaaaa cggtcaattg ctctaacttc
    11641 ccaatagctg tttgtgtgat ataaggtact aaattatctg gtctattcaa taacatttcc
    11701 cccaaaattc cacttggatg ggctccaact ggattgaagt atcttaataa aatagatttt
    11761 gtggtattga ctttagaata gtgttgtatg atttgttcgc cgatttgttt cgtatgtgca
    11821 tacgccgatt cagcttcttg aaaaggtgtt tcctccgtta caggcaatgc tttggcattt
    11881 ccatatacag aacatgaaga tgaaaataca aaatttggaa tgccaaattt ttgaatacct
    11941 tctaaaatat tatttaatga aattaagttg ttgttgtaat ataatattgg attagcaact
    12001 gattctggca ctgttttaaa tgctgcaaaa tgtataatac caacggcatc tttgtgttgt
    12061 tctaaaacat caaatgtttc ttgtttgttg cacaaatcaa tttgataaaa cttaaatttt
    12121 ttctctgtga tttgctcaat tgctccaatt ggatattttg ttgagcgtgc taaattatca
    12181 atgcatacca cttcaaagtc attttctaat aaatctacaa tggtgtgtga accaatatat
    12241 cctgttccgc ctgttacgat tactttcatc ttattatttt actaattcta ttaaatattg
    12301 tccgtagcca ctttttaata atggttgtgc tatttctttt aattggtcag ccgtgataaa
    12361 tcctttttta taagcaattt cttcaatgca accaactttt aaaccttgtc tttcttcaat
    12421 tacttgtacg aattgagagg cttgcatcag agaattaaac gtgccagtgt ctaaccaagc
    12481 ggtgcctcta tctaaaatgc caacttttag tttacctctt tttagatatt ctttattgac
    12541 atctgtaatt tcatattctc cacgaggaga tggctctagg ttctcggcaa tttcaacgac
    12601 atcattatca tagaaataca aaccaggaac ggcatagttc gatttaggat ttgttggttt
    12661 ttcttcgata gacacggcat ttaattgctc atcaaattca acaacaccat atctttctgg
    12721 gtctgaaacg tgataggcat aaattacacc accatcagga ttgttatttt ttaccaataa
    12781 atcttctaat cctgagccat aaaaaatatt atcacctaaa attaaagcaa ctttatcatc
    12841 gccaataaac gattttccaa ttacaaaagc ttgtgccagt ccattgggaa cttcctgaat
    12901 agcatattga aaattacaac catatttttt cccatcacct aataatcttt caaaatgagg
    12961 caaatcgtgt ggtgtagata taatcaatat ctcattaatt ccagcttgca tcaaaacaga
    13021 taacggatag taaatcattg gcttatcata tatgggcata atttgtttac tgatagccat
    13081 tgtaattgga taaagtcgag ttccagagcc tccagccaag ataatacctt tcatttttaa
    13141 ttatttttga tgattataaa aaattgttcg ctaatataaa cagaataaca gataaatttt
    13201 ttatgtttgt agaataaagt tttggcgata caaaattttg tgttaaattt gcattaaatt
    13261 agactatata tgagtacagg cgaaaaagtt tttaaggttt tagttgtaga tgacgaacaa
    13321 gacattttag aattcttaag ttataattta gaaaaagatg gatttcttgt ttctactgcc
    13381 gagaacggaa aagttgcttt agaaaaagca aaaaaagaag aaccagatat tattttatta
    13441 gatgtgatga tgcctgaaat ggatggaatt gaagcatgta gaacacttag agaaatgcca
    13501 caatttgaaa ataccattat tgcattttta acagcaagaa ctgaagatta ttctcaaatt
    13561 gcaggttttg aaactggtgc cgatgattat atcagtaagc caattaagcc aagagtatta
    13621 atcagtagat taagagcttt gttgagaaga tatgaaacca aagagtctaa agcttcattc
    13681 ttagaagtgg gcgatattaa aattgacaga gagcgttatt taattacgtt caaaggtaca
    13741 gaaatggcag tgcctagaaa agaatttgaa ttgttgtatt tattagcatc taagccaggt
    13801 aaagtattta aacgagatga aatcttaaat gagatttggg gtcgtgatat cattgttggc
    13861 gatagaacaa tcgatgttca tatcagaaaa ctaagagaaa agttgggaga agatttaata
    13921 aaaacagtaa aaggtatagg atacaaattt gatgaaatat gagaggaaac tcttctctaa
    13981 atacaatatc aatttataca agtatagcag tagcattttt ttctactgct ttttttttcg
    14041 tggttaatga gctatttgaa caccaaataa acatatttgc tcattttatt ttcttttttg
    14101 cgatattttt ctgttctttt attttgttta gatatgtatt aaagcaattg atttatgata
    14161 aaatcaagat tatatacaaa acaataggca aacctttaaa atttcagcat gaggtaaaat
    14221 catctaaagg aaatattatg ctgacagttg agcgagatgt tgctgaatgg gctatcaata
    14281 aaaacaagca aatacgtgaa ttacgaaaac tagagcaata taggaaagag tttgtgggca
    14341 atgtttctca tgaattaaaa accccaattt ttaatgcaca aggttatatt gaaactttat
    14401 tagattcaaa tttagaggat aaggatttta tgaaatctta tttaggaaaa gcagcatcta
    14461 atatcgatag attggaagtt attgttagtg atttgttaga aatttctaag tttgaaacgg
    14521 gtcgcataca attagaaaaa gatgggtttg atattgtcaa attaattaaa aaagtaattg
    14581 ctcagtatca atttttagct gaagattgta aagtaaaagt aaaagttttt aatcatgaga
    14641 atgaattttt tgtatttgct gatgaaaaaa gaattatgca ggtattggaa aatattattt
    14701 ctaattcaat aaaatataat aatgaaaaag gcaaagtaga tattagaatt tatgatttag
    14761 atgaacaata tatgatagaa ataagcgata atggaccagg aattgcagaa gagcatttgc
    14821 ctagattatt tgaacgtttc tatcgtgcgg ataaaagtag agcaagaaaa gttggaggta
    14881 caggacttgg actatctatc gtaaagaata ttattgaagc acatgaagaa acaataaatg
    14941 tgagtagcac tttaaatgaa ggcactatgt ttagttttac tttacataaa tatcgagaaa
    15001 gattataaag tatctaaagc cgccaaaaga atagaacaac tagcttgtat ttcatcttca
    15061 gtaataatta atggcggagc aatacgcaga caattattgg caaacaagaa ccaatctgta
    15121 attagtccat tttttaaaca atattgaatg acttgatgta tttgttcgaa actatccaaa
    15181 taaacagcca tcaataagcc gaaattatta atttttttaa tctttggatg ttttaataat
    15241 tgcaggaaca atgctgcctt tttgggtaca tcaatatata attcttgttg atgaatggtt
    15301 tgaatattgg ctaatgccgc agcacagcaa acagcatttc caccaaatgt agtaatatga
    15361 ccgagaacag gattgtgtgt taaactttgc agaattttct tgtcagaaat aaagcaacca
    15421 ataggcatgc ctccacctaa tcctttagcc aataaaataa tatcaggcaa aataccaaaa
    15481 tgctcaaagg caaatagttt tccagttcgt ccgcaacctg tttgaatttc gtctaaaact
    15541 aataatgtgc ctgtttcgtt acatttttgt cttaccgcaa tcaaaaattc tttagttgga
    15601 gcaataacac cagcttccgc ttgaacactt tctaaaaaaa ttgctgcagt tcgttctgta
    15661 atcaattcta tatcctctaa attattatac ctgatttgtc gagtatctgg cagtagtgga
    15721 cgaaatgcag atttgaaaaa ttcatctccg agcaagctca atgctccttg tgtacttccg
    15781 tgataggcgt ttacacaaga aataatctca ggtttgccag taaatcgctt tgctaatttc
    15841 atggcacctt cggttgcttc agcaccacta ttggtaaaat aaatactgtt caaattttca
    15901 ggtagcaaat ctgatagata ctttgctaat tgaacttgtg gcgattgtat aaattcgcca
    15961 tataccaaag tgtgcatata ggtttgagct tgttgctgta tcgcatttac aacatttgga
    16021 tggcagtgtc ctatattact tactgaaatt ccagctatta aatcgatgta ttttttaccc
    16081 gaaatatcat acaaatatat accttcagct ttttctattt ctaatgctaa tggctcaggt
    16141 gatgtttgtg caacatgttg taaaaataat tgtcgttgtg atagcatttt tttgattaac
    16201 ttttattaag tgcataaaat aaaaaaacca tacatttatg ttttattaca aatgtatgga
    16261 gtttatagtt tgtaaagcgt taattactct tctataacag aaaccttcaa cattctaatg
    16321 tcattaaatg gacgatcata aggatctcga ggtgcagaaa caattttatc aacaatatct
    16381 tgtccttcta cagtttgtcc aaatacggta tattgcatat ctaacattgg tgtaccacca
    16441 attgtcttgt aaatttcttt ttgtgcttct gtccatgaat tgccagttct tgaacctaaa
    16501 atatttaatt cattatctgt ataagttttc ccaacaacaa tataaaattg tgtacttgaa
    16561 gaagcttttt gaggattgcc atcacgagca gcagccaaag ctcctttttg atgaatgata
    16621 tttggaaata attcagcagg aactctataa tctaaaccgc ctgttcctaa acgctgtcct
    16681 ggtactgcat ttttagataa tggatctcca ccttgaatca taaattcagg aataacacgg
    16741 tggaataagg tgctatctaa aacgccatct tgtacaattt taataaaatt ggctttgtgc
    16801 aatggggttt catcaaatag ttttatttta atgtttccga ctgttgtttc gatgacaaca
    16861 taagttgttt ttggggtact aatttttggt tccatttttt ctacaattgt atctaatgtt
    16921 attatttctt ctttaattgt tttatctaca gtttctaatt ttgaagtaga ttttatgttt
    16981 tttgtttttt ttccttttgc tatggctaaa cctgaacaaa gtaaaagtgc gaggataagt
    17041 aaaagtgttt tttgcatatt ctttatattt ttaattagtc aattatagct ttttgatgta
    17101 ttgaataatc atttttttta agagcatttc ttgttgttcg tgggttaatt tctctttgtt
    17161 tctgttgtat tcataatgtg gccatccatc ttcatcaatg cctttgaaag tatagtaatc
    17221 tgcaagactt aataatgcac acacaccgac atgtatcaca tcttgttttt gttctttgga
    17281 tagtttgact aaattttgtc caatttcccg atggccgatg atgaacaaca tattcgttaa
    17341 atcaggaatt ttatcaaaac gatttttaaa aaaatcagtt acttttttcc aatcttctat
    17401 actaaaatct aatgcttcac tattcgctat ttcactcatt ttttatccaa acatttataa
    17461 attaaattgt ctaagccgta aatgcttaga caatttattt gtttaaaaat tacttttctt
    17521 ctttaatttg ttttttcttt ttggacttaa cactgaaagt caatttttct ttgtcttctt
    17581 tgtccgtatc aactagaata gtatcgccat tttgaatttt attttgaaga atttcttctg
    17641 ctaatggatc ttcaacatat ttctgtattg cacgatgtaa
//
LOCUS       DAOQCW010000043        17589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1150_length_17589_cov_4.991502, whole genome shotgun sequence.
ACCESSION   DAOQCW010000043 DAOQCW010000000
VERSION     DAOQCW010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 17589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17589
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1150_length_17589_cov_4.991502"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..994
                     /locus_tag="PLS16_05385"
     CDS             98..994
                     /locus_tag="PLS16_05385"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNJ60546.1"
                     /translation="MKKICLLLSFIGLFTHLFSQNKKLLIIGLDGCRADVVNHRYAPT
                     MDSIIHLKNTAYSYKMRNERRTMSATNWSSLLTGVHFSKHKAKNNRKFYKGDFVNYPP
                     FMKYLKDENPELKCASYSTWGDINKYIMLDYLDDITNANKDLADSVVENKVLSMLKLP
                     EVYDATFVHLEDIDHNGHLKGFSKNNATYTDAVTRKDSFVKEILSIVQERKIKYIEDW
                     LVIITTDHGGRRIGRASGHSFGMINPNIRQNFLIMNGDSVNSGKIIAPRTIDIAYSIL
                     TFFGVKIKPEWNLQGKTIGLKD"
     gene            complement(998..1996)
                     /locus_tag="PLS16_05390"
     CDS             complement(998..1996)
                     /locus_tag="PLS16_05390"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004042.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HNJ60547.1"
                     /translation="MINACTNVSSKENFKVMPTDNNTTLLSEQEWKAKLSDEEYYILR
                     EKGTEKPFTGKFLMHKENGYYTCKGCGEKLFKSDAKFDAHCGWPSFDAEIEKGKITYT
                     QDNSHGMQRVEITCSKCGSHLGHVFNDGPTKTGLRYCVNSVSLEFEPESTKTTDTMTI
                     GGGCFWCTEAVYRRINGVLKVEPGYAGGHTKNPTYKEVCTGNTGHAEVIQLTYNPSVI
                     SYLELLKVFFATHDPTTLNRQGNDVGTQYRSVIFFHNEQQKQIATEVISELTLNQIYK
                     QPIVTEIVPITNFYNAEDYHQNYFEENSTSNPYCEIVIQPKVEKFEKLFKNLLKGK"
     gene            complement(2031..2639)
                     /locus_tag="PLS16_05395"
     CDS             complement(2031..2639)
                     /locus_tag="PLS16_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNJ60548.1"
                     /translation="MKKRKVIKENDAKLKMVHYLSEQFNIDPEIINILVDIFKLKKYK
                     KKELIINDTENIDKVCYIVDGLVRIYYHRENKEITNWFIEENGVFMPVYHILTGQPNN
                     NNYEALKTTIVLESSYSKLEKIYAFHPQLQLLGRKMIESYYAKFLAHSHNVLFLSAEE
                     RYASFIEKKSDLLNIVALKHIASYLGISQETLSRIRAKKMVE"
     gene            complement(2712..3062)
                     /gene="rplS"
                     /locus_tag="PLS16_05400"
     CDS             complement(2712..3062)
                     /gene="rplS"
                     /locus_tag="PLS16_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNJ60549.1"
                     /translation="MDKIKELEKSLVANVEHPEFKAGDNVSVHYKIKEGNKERIQIFK
                     GDVIQINSTGVNKTFTVRKISNGVGVERIFPYFSPNIAQVEVHKVGKVRRAKIFYIRE
                     LKGKASRIKEKIVK"
     gene            complement(3127..3816)
                     /gene="trmD"
                     /locus_tag="PLS16_05405"
     CDS             complement(3127..3816)
                     /gene="trmD"
                     /locus_tag="PLS16_05405"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNJ60550.1"
                     /translation="MRIDILSAVPNLLNSFFEHSILKRAIEKQLLEVHIHNVRDYTNH
                     KQKQIDDYQFGGGAGMVMMIEPILNCIEHLQKDRKYDEILYTTPDGELFNQAIANSLS
                     LKENIIIVCGHYKGIDERLREHIITKEISIGSFVLSGGELPAAIIVDAIGRIIPGVLN
                     DETSALTDSFQDNLLAPPVYTRPADYKGWKVPEILLSGHEKKIEAWRYEKSIERTKAR
                     RPDLLRENEIE"
     gene            complement(3813..4319)
                     /gene="rimM"
                     /locus_tag="PLS16_05410"
     CDS             complement(3813..4319)
                     /gene="rimM"
                     /locus_tag="PLS16_05410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNJ60551.1"
                     /translation="MTSQNIQIGKVLKTHGTNGNLKVFIEALFFKEALKSNHFFINQL
                     PYFVESIQKVGEQEIILKLEDVDSRETAQNLCGFEILMDRQQVKKWKKQIFDETLINF
                     TIYNQDLIIGKVQDILEMPQQIMLVTSIQNKEVLIPLNEDFLVEINPNEQKIVLNLPE
                     NYFEIFLG"
     gene            complement(4379..4876)
                     /gene="rpsP"
                     /locus_tag="PLS16_05415"
     CDS             complement(4379..4876)
                     /gene="rpsP"
                     /locus_tag="PLS16_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNJ60552.1"
                     /translation="MATRLRLQRHGSKGQPFYKIVAADSRAKRDGRFIEQIGTYNPTS
                     IPATIVLDIDKAVKWVQTGAEPSDTVKAILKYKGVLYKNHLLRGVKLGLFAAEEVETK
                     FNAWLDAKEGKVAEHKAKADADKKAKHAISVNAKGKVKKVVEETPAEESAPAEETEAP
                     QESAE"
     gene            complement(5016..6614)
                     /gene="aceB"
                     /locus_tag="PLS16_05420"
     CDS             complement(5016..6614)
                     /gene="aceB"
                     /locus_tag="PLS16_05420"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF013442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HNJ60553.1"
                     /translation="MAQYPQLEGIEIKGEYRPEWAEIITPEAVAFFAKLQRKHNARRK
                     ELLQKRVERQVRLDAGELPDFLPETKEIRESAWTVAPVPADIQDRRVEITGPVDRKMI
                     INALNSGAKVFMADFEDSNAPTWENNIQGQVNLRDANRKAISFVNEANGKEYKLNEKH
                     AALFVRPRGWHLLEKHVIVDGEEVSGGIWDFALYFFHNVKQRLENGSGLYLYLPKMES
                     HLEARLWNAIFVDAQNELGVPQGTVKATVLIETIVATFELNEILYELKEHSAGLNCGR
                     WDYIFSFIKRTVKNKAFLVPDRTQVTMTTHFLKSYSELVIQTCHKREAFAMGGMAAQI
                     PIKDNPEANEIALAKVRADKEREAKAGHDGTWVAHPGLVQIAMDIFNEYMPTPNQIFN
                     KREDVHVTAADLLKVPEGTITLGGLKNNIDVGIQYLEAWLRGNGCVPIHNLMEDAATA
                     EISRTQVWQWVHHEDAKLDDGTKVTKELVQKLIDQQKEAYKTSFGDKYAATKFDLAID
                     LFTKMSLQDTLEEFLTLEAYNYID"
     gene            complement(6754..7875)
                     /locus_tag="PLS16_05425"
     CDS             complement(6754..7875)
                     /locus_tag="PLS16_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNJ60554.1"
                     /translation="MAKSKSSSKKATKQPIYSIDKKAENFLFKYLNNASPTGFEAEGQ
                     KIWLDYIKPYIDKYEVDYYGTTYGIINPDAKFKVVIEAHADEISWFVSYIGEDGLINV
                     IRNGGSDHMIAPSKRVNLHTKNGIVKGIFGWPAIHLRDASKDTAPSVKNITIDVGAAN
                     KKEVEDMGIHVGTVITYEDELMILNNKFYTGRALDNRMGGFMIAQVARLLSENKDKLP
                     FALYIVNSVQEEIGLRGAQMVVERIKPNLAIITDVCHDTTTPMIDKKVSGDFKCGNGP
                     VITYGPAVHNILREQILDTAEKNEIAFQRDAASRSTGTDTDAFAYGNAGVPSALISLA
                     LKYMHTTVETVAKSDVEAVISLIYETLKNINPKTSYKYL"
     gene            8065..8856
                     /locus_tag="PLS16_05430"
     CDS             8065..8856
                     /locus_tag="PLS16_05430"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNJ60555.1"
                     /translation="MNSAKRKVEICIISDIHLGTHACHAKELNRYLNSIEPNILIING
                     DWLDIWHFSVGYFPTEHVENIFLILDFARRGIPVYYITGNHDDSMRDFSGYRFDVLEL
                     RDEIILEIDGKKHWIFHGDKYDLSVGGSARWLAKLGGRYYDNIFRINRYINKIRMQQG
                     KEEIFISKFIKDKIKSIVKSKVQDFEEVAIDIAIEEKYDYVICGHIHKPQIRTITKSN
                     GSTTYLNSGDWVENLTALEYNNGKWDLFYYRHDLEFKGIDRTPRD"
     gene            8862..9191
                     /locus_tag="PLS16_05435"
     CDS             8862..9191
                     /locus_tag="PLS16_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015740450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HNJ60556.1"
                     /translation="MGYVYLALAIILEVLGSSLIKVSDGFTKIIPTSIMLIAYVTCFY
                     FLSLTLKTIPLGIAYAIWGGLGIVLTAIISVVIFKQNLDLPAILGIALIVSGVFIMNF
                     FSKTNAH"
     gene            complement(9225..9983)
                     /locus_tag="PLS16_05440"
     CDS             complement(9225..9983)
                     /locus_tag="PLS16_05440"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HNJ60557.1"
                     /translation="MSEIRENEHGELDVRVVAPIQRHEFLVELFKNLPVAESFVFIND
                     HDPIPLFYEFKSIYGDVVDWVYLNRGGREWKVKVTRTAASEERDMKDISTLLDLRKAA
                     PKEWKQIVFHRYGMMEPDTTMELISSIDPEEIQQIFKNKFEGQYTWTYKRQVPDEYII
                     HIKKVEKSGLGDDGFSVVNEMDIRPFPPAERHEMFYKAFADIKVGEAFEFINDHDPKP
                     LYYQMEAESKEPFRWEYIENGPDAWKVRVVKVKA"
     gene            complement(10123..11223)
                     /locus_tag="PLS16_05445"
     CDS             complement(10123..11223)
                     /locus_tag="PLS16_05445"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNJ60558.1"
                     /translation="MKTKFCILMFFSLLFIFQSKAQSTSNWSNQYDEVGKFYDGLAWV
                     KLDYKYGFVDQTGKKVTPLKYDFVGDFSEGLAMVMTGDFEGLEEPKCGFINKTGTEVI
                     PLKYSKAEKFSEGLAMVVEGILGDALSGFIDKTGKVIIPIQYEDAGNFSEGLAKIQLN
                     ENWGFIDKTGKELVAPKYNSVGDFSEGMAVVSSGTSGVEKFGFIDKTGKEIIPLKYDY
                     AGKISEGLASVSINDKYGYIDKTGAEIIHLQYEDASDFSEGIAAIAQNNYKYSFIDKT
                     GKEVIASDYDYAGNFSEGLVMVRKGDYSSGKWGFIDKTGKVIIPLIYSETEPFAQGLA
                     RVKTDENWFLINPKGECVSGCKYAPTTHLKAK"
     gene            11617..12411
                     /locus_tag="PLS16_05450"
     CDS             11617..12411
                     /locus_tag="PLS16_05450"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNJ60559.1"
                     /translation="MFIQQFPIPHSLQNHIVNISFYKLTKDEMLRRKILPDNITSLFV
                     FLNPTIHLKECFSNSIINYRVAYAGPYSKPRLYEVEEEIRFIKIDFFPWGAFSAFGLE
                     QYKLHNTTIDAYKLFPKLKLYSKPLEASLDNIETCIAILEQFMFEDCHEYLTPTDEKI
                     ITACNLIKSKDKNIPIKELCTLVNMSQNSFCNHFTKIIGISPKLFGQIARYSKAKKII
                     HSTIDINWNEIAFQFGYFDQSHFIKEYKRFSGAIPKEYKAWKNFYN"
     gene            12512..15001
                     /locus_tag="PLS16_05455"
     CDS             12512..15001
                     /locus_tag="PLS16_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60560.1"
                     /translation="MKKVIIYFSLSAILIFGCKKDKISNTPTPPPKGQLEIKIQVPAN
                     TDLEVNGGEVLSGATFVDIKDKNKVPIPVFKDDITPVYVFDNKDNLVMMGFAGDNARI
                     VSSATTAQVLLYWGLGLPYADVAFTKSFFEQYEGNKIVGQWVQKFEQDFIADPLIISK
                     GGFIDGLTATVKELRNKDVLDANNMKKSADIKVDAANVKSGLRVQEDGLSQYSVNNYY
                     RRRAHAYLYKMNYTDMNNSKHTLISQFGENTKAENDFPVSSRSGVTSVMGEIGKWVDG
                     SDMGSMVAKTDPNQLQLEDNESEALYKLRIVGPGSLAGAHMSGNEATTAELSKYLELS
                     AETFLLDFLIPTLSLITSAEGMTADFDWSKKDIDIAIDAIKSFWKTTPDVYEEIKKGS
                     YSNALKKVITSIGADVANTYLTAIFDGIGDMGLKNLWSGSVTHIKISKVLAVVDFILG
                     TTDLALTTNNILSSKGIESFDITARTGKVTLLPVEATGVSGSFIKLEAIIKNMELPQG
                     THPFFEWSTSGNFGELWDNQGHRSVSFESQDAKPQYYVKASSNQLSDGNNFEYVYVKA
                     YIGSNVVGTDTTKINVKKVSYKILPTGVTISGQKDKANEIRLYLVRSDNKKDIGQAGD
                     YDYKVVWETAGSYGKLKSGFNNSTTITTYNSDNITYSCTDEDTKEAQEEIRAKVYMKQ
                     KTAPDDEYFLASDTKGIVKIGNDENKKIIHIPMTVFIYQFPDQDNWCGQYPMAIFPVE
                     DSAKHYQVVFYNFPPNYILDGRIIEKITYSWAKGQRPNNEYNPFHGENPPLVHNGQYY
                     ISFAWTGGNCDWINTLPSDNGGVMVEVTYYY"
     gene            complement(15152..15745)
                     /locus_tag="PLS16_05460"
     CDS             complement(15152..15745)
                     /locus_tag="PLS16_05460"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNJ60561.1"
                     /translation="MIEIRFADFLSSNSTLEPTLMEALLNRCKNVHIKKGDFWLRAGE
                     ACQQTLFVENGLLRYYSIDDKGKEHLLQFAPEGWFVSDRASIFFNEPSAYFIQALEDT
                     EAIILDESFMLDLAKKDVSFMEFNNKLLHNHIRQLQNRINQLLSASAEERYLHFTRIY
                     PDILLRVPQLMVASYLGITPESLSRVRKELAQRNHKS"
     gene            16029..>17589
                     /gene="cas12a"
                     /locus_tag="PLS16_05465"
     CDS             16029..>17589
                     /gene="cas12a"
                     /locus_tag="PLS16_05465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V CRISPR-associated protein Cas12a/Cpf1"
                     /protein_id="HNJ60562.1"
                     /translation="MANLLDFTDKYQLSKTLRFELKPIGRTLEHIQTKGLLLQDANRA
                     KSYQEVKKLIDRYHQHFIELALSPLKLTQLEDLQNLYFAPAEAKKEDDFKKKFEKIQE
                     ELRKEIVTAFKTGESNEIFKVLDKKELITKLMVDWLEQKEDKDKVEEFKTFTTYFTGF
                     HENRKNMYTDKAQSTAIAYRLVHENLPKFLDNIKVFEKLKAVPELYEKCSVLYKEIEE
                     YLNIRYIDEAFELGYYNMVLTQKQIEVYNLIIGGRTPKENEKKIQGLNEYINLYNQKQ
                     ADKKNRIPKIKPLYKMILSDRESVSFLAEKFDNSQEVLNAIKEYYHANIKSYQSSEKD
                     DTENVLERVKDLLANLSSNNLFQIYIRNGKAITDISQALFGDFSLIKDALKANFEKTL
                     PEPKGKSKDTRPEKIEKYLKQDYFSIAEIETALWNYRAENDLLKDLKEEEHLIASYFN
                     SHFKTTVNDKEYDLVANVMAKYSCIKGLLEQDYPEDKKLNQETKEIDEIKAFLDALML
                     VLHFVKPLMLPN"
BASE COUNT         5838 a         2756 c         2736 g         6259 t
ORIGIN      
        1 agataaagaa attaatattt cttaggagta aaaattcata acggtccata gttagaagta
       61 ttgagcaata gtttatacta atattaaaag aatttgtatg aaaaagattt gtttattgtt
      121 gtcttttata ggtcttttta cacatttatt ttcacaaaat aaaaagttgt taattattgg
      181 attagatggt tgccgagcag atgttgttaa tcacagatat gcaccaacga tggatagtat
      241 tattcattta aaaaatacag cctattctta taaaatgcgt aatgaaagac gtactatgag
      301 tgccacaaac tggagtagtt tacttacagg tgttcatttt tctaagcata aagccaaaaa
      361 taatagaaaa ttttataaag gagattttgt gaattatccg ccttttatga aatatttgaa
      421 agatgaaaat cctgaactta aatgtgcttc ctattcgact tggggggata tcaacaagta
      481 tattatgttg gattatttag atgatattac gaatgctaac aaagatttag ctgattctgt
      541 agtagaaaat aaagtattaa gtatgcttaa attacctgaa gtttatgatg ctacttttgt
      601 tcatttagaa gatatagacc acaatggaca cttaaaagga tttagtaaaa ataatgctac
      661 atatacagat gctgttacaa ggaaagatag ctttgtaaaa gagattttat ctattgttca
      721 agaaagaaaa attaagtata ttgaagattg gttagtaata attacaactg accacggcgg
      781 cagacgtatt ggtcgtgcga gtggacatag tttcggaatg ataaacccaa atatccgaca
      841 aaattttctt attatgaatg gtgatagcgt taattctgga aaaataattg ctccaagaac
      901 aatagatata gcttatagca ttctaacatt ttttggtgta aaaattaagc cagaatggaa
      961 cttgcaagga aagacgatag gtttaaagga ttaatgttta tttgcctttc aataaattct
     1021 taaataattt ttcaaatttc tccacttttg gctgaataac aatttcacag taaggattgg
     1081 aagtgctatt ttcttcaaaa taattctgat ggtaatcttc tgcattatag aaattagtaa
     1141 taggaactat ttctgtaact attggttgct tgtaaatttg atttaaagtt agttcggaaa
     1201 taacttcagt tgcaatttgt ttttgttgtt cattatggaa gaaaattaca cttctatatt
     1261 gagtgccaac atcgttgcct tgtctgttta aagtagtagg gtcgtgggta gcaaagaaaa
     1321 cttttaataa ttccaaatag cttattacag atggattata ggtaagttga atcacttcgg
     1381 catgtcccgt attgcctgta caaacttctt tatatgttgg attttttgta tgaccgccag
     1441 cataaccagg ttctactttc aatacgccat taattctacg ataaacagct tctgtacacc
     1501 aaaaacaacc accgccaatg gtcatagtat ctgttgtttt tgtactttca ggttcaaatt
     1561 ctaatgaaac agaattaaca caatatctaa gtccagtttt tgttggtccg tcattaaaaa
     1621 catgtcctaa atgactgccg catttactgc aagtgatttc tacacgttgc ataccatgag
     1681 aattgtcttg cgtataggta attttgcctt tttctatttc tgcatcaaag cttggccaac
     1741 cacaatgtgc atcaaatttt gcatcacttt taaataactt ttcaccacag cctttgcagg
     1801 tataatatcc attttcttta tgcattaaaa atttgccagt aaaaggcttt tcagttcctt
     1861 tttctcttaa aatataatat tcttcatcgc ttagcttagc tttccattct tgttcggata
     1921 ggagagttgt gttgttgtca gtaggcataa ctttgaaatt ttctttagaa gatacattag
     1981 tacaagcatt aatcataaat gatattagaa tgtaataaaa attcagcatc ttattcaacc
     2041 attttttttg ctctaattct actcaaggtt tcttgtgaaa ttcccaaata tgaagcgata
     2101 tgctttaaag caactatatt taataaatct gatttttttt caataaaact tgcatatcgt
     2161 tcttcagcag ataaaaataa aacattgtgc gaatgagcca agaacttagc atagtaactc
     2221 tcaatcattt ttctacccaa taattgtaat tgaggatgaa aggcatatat tttttctaat
     2281 ttgctgtatg aagattctaa aactatagtt gtttttaatg cttcgtaatt gttattgtta
     2341 ggctgaccag taagaatatg ataaacaggc ataaaaacgc cattttcctc aataaaccaa
     2401 ttggtaattt ctttattttc tctgtggtaa taaatacgta ctaagccatc tacgatataa
     2461 cataccttat ctatattttc agtatcattg ataattaact ctttcttttt atacttttta
     2521 agtttaaaaa tatcaactaa aatatttatg atttctgggt ctatattaaa ctgctcgctt
     2581 aaataatgaa ccatttttag tttggcatcg ttttccttta taactttgcg tttcttcata
     2641 cttcaatata acaaaaaagt cccaactttg ttggaacttt ttttattttt tttgtgtttt
     2701 ttgaaaaata attacttaac gattttttct ttaattctag aagctttacc ttttaactct
     2761 ctaatataga atattttagc tcttcttact ttaccaactt tgtgtacttc tacttgtgca
     2821 atatttggtg aaaaataagg gaagatacgt tcaacaccaa cgccatttga aattttacgt
     2881 actgtaaaag ttttatttac gccagtacta ttgatttgaa ttacatctcc tttaaaaatc
     2941 tgaattcttt ctttgtttcc ctctttgatt ttataatgta cactcacatt gtcacctgct
     3001 ttaaattcag gatgctcaac attagcaact aaactctttt ctaattcttt aatcttatcc
     3061 attgtaatgt aatttaagta aattgagtgg caaaggtaat tttattttct gaattatcct
     3121 aattttttat tcgatttcat tttctcttaa caagtcaggt cttctggctt ttgttcgttc
     3181 aatgcttttt tcatatcgcc atgcttcgat ttttttttcg tgtcccgata agagaatttc
     3241 agggactttc caacctttat aatctgctgg tcgagtataa acaggtggag ctaataaatt
     3301 atcttgaaat gaatcggtta atgcacttgt ttcatcattt aaaacgcctg gaattattcg
     3361 accaatagca tcaacaataa tagcagcagg taattcgcct cctgaaagta caaaactgcc
     3421 tatggaaatt tctttggtaa taatatgttc tcgtaatcgc tcatctattc ctttgtaatg
     3481 cccacaaaca atgatgatat tttcttttag tgataaacta tttgcaatgg cttgattgaa
     3541 taactcgcca tctggagtag tgtacagaat ttcatcatat tttcggtctt tttgtaaatg
     3601 ttctatgcag ttcaaaatgg gttctatcat cattaccatg cctgcaccac ctccaaattg
     3661 gtaatcatca atttgttttt gtttatggtt tgtataatct ctaacattgt ggatatgaac
     3721 ttccaatagc tgtttctcta tagctctctt tagaatagaa tgttcaaaaa aactattcaa
     3781 taaatttgga acggcactta aaatatcaat tctcatccta aaaatatttc aaaataattt
     3841 tctggtaaat ttaaaactat tttttgctca tttggattaa tctccaccaa aaagtcttca
     3901 tttaatggaa tcaaaacctc cttgttttgg atggaggtta ccaacataat ttgttgtggc
     3961 atttctaata tatcttgaac tttcccaata attaaatctt gattgtatat tgtaaaatta
     4021 attagtgttt catcaaaaat ttgttttttc cactttttta cttgttgtct atccatcaat
     4081 atttcaaagc cacaaaggtt ttgtgctgtt tctcttgaat ctacatcttc taattttagt
     4141 ataatttctt gttcgcctac tttttggata gactcaacaa aatagggaag ttgattaata
     4201 aaaaaatggt tgctttttaa agcttcttta aaaaataggg cttcaataaa aacttttaga
     4261 ttaccgtttg tgccgtgtgt ttttaaaact tttcctattt gtatattttg gctagtcatt
     4321 gtctaaataa aaaatcctga ccataaagat caggattaaa acttaattta ttttaatatt
     4381 attctgcact ttcttgtgga gcttcagttt cttcagcagg agcagattcc tcagcaggag
     4441 tttcttcaac tactttctta acttttcctt tagcatttac tgaaatagca tgttttgctt
     4501 ttttatcagc atcagctttt gctttatgtt cagctacttt gccttcttta gcatctaacc
     4561 aagcattaaa tttagtttct acttcttcag cagcaaataa acctaatttt acacctctta
     4621 ataaatggtt tttgtataaa acacctttgt atttaaggat tgctttaaca gtgtcagatg
     4681 gttcagcacc agtttgtacc cattttactg ctttgtcaat gtctaatacg attgtagcag
     4741 gaattgaagt tggattgtac gttccaattt gttcgatgaa tctaccatct ctttttgcac
     4801 ggctgtccgc agctacaatt ttgtagaaag gttgtccttt tgaaccgtgt ctttgtaatc
     4861 ttaatcttgt tgccataaaa atgttttttt tttgttttta gtttgctttt gctgaaaaat
     4921 cagccttgca gttttaaact tttgagtggc aaaggtaata atattttaaa tagcttgtat
     4981 attacaatat tattacctgc caattgctca atttattagt caatatagtt gtatgcttct
     5041 aatgtcaaga actcttctaa agtatcttgt aagctcattt ttgtaaataa atcaattgct
     5101 aaatcaaatt tagtagctgc atatttatct ccaaaactag ttttataagc ttctttttgt
     5161 tgatcgatta gtttttgaac taattcttta gttactttag ttccgtcatc taatttagca
     5221 tcttcgtggt gaacccattg ccaaacttgt gttcttgaaa tctcagcagt tgcagcatct
     5281 tccatcaaat tatggatagg cacacagcca tttcctctta accaagcttc taaatattga
     5341 ataccaacat cgatattatt ttttaaacca cctaaagtaa tagtaccttc aggcactttc
     5401 aataaatcag cagcagtaac atgaacatct tcacgcttat tgaaaatttg atttggtgtt
     5461 ggcatatatt cattaaaaat atccattgca atttgaacta atccaggatg tgctacccaa
     5521 gtaccatcat gtccagcttt tgcttcacgt tctttatctg cacgtacttt tgccaatgcg
     5581 atttcatttg cttcaggatt gtctttaatt ggaatttgtg ctgccattcc gcccattgca
     5641 aaagcttctc ttttatgaca agtttgaatc actagttctg aataagattt caagaaatgt
     5701 gttgtcattg taacttgtgt tctatctgga actaaaaatg ctttgttttt tacagtacgt
     5761 ttgatgaatg agaaaatata atcccaacgt ccacagttta atccagcaga gtgttctttt
     5821 aattcatata aaatttcatt taattcaaat gtcgcaacga tagtttctat caatacagtt
     5881 gcttttacag taccttgtgg tacgcctaat tcattttgtg catcaacaaa aatagcattc
     5941 cataatctgg cttctaagtg agattccatt tttggtaagt ataaatataa tccactacca
     6001 ttttctaatc tttgttttac attgtggaag aaatataaag caaaatccca aataccgcca
     6061 ctcacttctt cgccgtcaac gataacatgt ttttccaata aatgccatcc tctaggacga
     6121 acaaataaag ccgcatgttt ttcgtttaat ttatattctt ttccattcgc ttcgtttaca
     6181 aaagaaattg cttttctatt tgcatcacgt aaatttactt gtccttggat attgttttcc
     6241 caagtaggag catttgaatc ttcaaagtca gccatgaaaa cttttgcacc agagttgagt
     6301 gcattaataa tcatttttct atcaacagga ccagtaattt caactcttct atcttgaatg
     6361 tctgcaggaa ctggagctac tgtccaagct gattctctaa tttctttagt ttcaggtaaa
     6421 aaatcaggta attcgccagc atctaaacga acttgtcgtt ctactctttt ttgtaataat
     6481 tccttacgtc ttgcattgtg ctttctttgt aattttgcga aaaatgcaac tgcttcaggc
     6541 gtaataattt ctgcccattc aggtctgtac tcgcctttaa tttcgatacc ttctaactga
     6601 ggatattgtg ccataatagt atgttgttta gtttaaaaat ctaatattcg caaatataag
     6661 aaatttaaaa gtcaaataaa aactaagatg ttaagcctat tttaaactta gttgtattgg
     6721 aataaagaaa acctagctac tttcttatta ttactataaa tatttataac tagtctttgg
     6781 attgatgttt tttagtgttt catagattag tgaaattaca gcttccacat cagattttgc
     6841 aacggtttca actgttgtat gcatatattt taaagccaat gaaatcaaag cacttggtac
     6901 gccggcattg ccataagcaa aagcatcagt gtctgttcct gtacttctac ttgcggcatc
     6961 tctttgaaat gcaatttcgt ttttctcagc ggtatctaaa atttgttccc ttaaaatatt
     7021 atgcacagct ggaccataag taatcacagg accattgcca catttaaaat caccacttac
     7081 ttttttgtct atcattggtg tagttgtatc gtggcaaaca tctgtaatga tggctaaatt
     7141 tggtttaatt cgttctacga ccatctgagc accacgcaat ccaatttctt cttgaactga
     7201 attgacaata tataatgcaa aaggcaactt gtccttattt tcactaagca atcgagccac
     7261 ttgagcaatc ataaatccac ccattcgatt atccaaagct cttcctgtat agaatttgtt
     7321 attgagtatc attaattcat cttcataagt aatgactgta ccgacgtgta tgcccatatc
     7381 ttccacctct tttttattag ctgctcctac atctatcgta atatttttta cagacggagc
     7441 tgtatctttt gaagcatctc ttaaatgaat tgcaggccaa ccaaaaatac cttttacaat
     7501 accgtttttg gtatgcagat ttactctttt tgaaggtgct atcatgtgat cactaccgcc
     7561 atttctgata acattgatta gtccatcttc gccaatataa cttacaaacc aagaaatttc
     7621 atctgcgtga gcttcaataa cgactttaaa ttttgcatct gggttaatga tgccataagt
     7681 tgtaccataa taatctactt cgtatttatc aatgtatggt ttaatataat ctaaccaaat
     7741 tttttgacct tctgcttcaa aaccagttgg tgatgcattg ttgaggtatt tgaataggaa
     7801 attttctgct tttttgtcta ttgaatagat aggttgtttt gttgcctttt ttgaacttga
     7861 tttgcttttt gccataagga ttgaatttag aacaacaaaa gtaaatatta tttcaatttt
     7921 ataattgcta aaattgttta ataattgaaa cctttatact ttgatgagtt ttagataaaa
     7981 gagttggcta atttgtaatt attgttttat aaaatctatt gctttactgt gattttgata
     8041 atttattttg tataattgca ttttatgaat tcggccaaga gaaaagtaga aatctgtatt
     8101 atttccgata tccatttagg tacacatgct tgtcatgcaa aagaattgaa tagatatctg
     8161 aatagtattg agccaaatat tttgattata aatggtgatt ggttagatat ttggcatttt
     8221 tctgtgggtt attttccaac cgaacatgtt gaaaatattt tcttaatttt agattttgct
     8281 agacgaggaa ttcctgttta ttatattact ggaaatcatg atgattctat gcgtgatttt
     8341 tcaggctatc gttttgatgt tttagaactt cgtgatgaaa ttattttaga aattgatgga
     8401 aaaaaacatt ggatttttca tggcgacaaa tacgatttgt ctgttggtgg aagtgcaaga
     8461 tggttagcaa aattaggcgg tcgttactat gataatattt ttagaatcaa tagatatatt
     8521 aataaaataa ggatgcaaca aggtaaggaa gaaatcttta tttctaaatt tatcaaagat
     8581 aagataaaaa gtattgtaaa atcaaaagta caagattttg aagaagtcgc tattgatatt
     8641 gctattgaag aaaaatatga ttatgtgatt tgtgggcata tacacaaacc acaaatccga
     8701 acgattacaa aatctaatgg ttcaactact tacttaaatt ctggcgattg ggtggaaaac
     8761 ttgactgctt tagaatataa caatgggaaa tgggatttgt tttattaccg acacgattta
     8821 gagtttaaag ggattgatag aacaccaaga gattaatttt tatgggatat gtttatttag
     8881 ccttagcaat tatattagaa gtattaggct ctagtttaat aaaagtttca gatggattta
     8941 ctaaaataat tccaacatca attatgctta ttgcttatgt aacttgtttt tattttttat
     9001 cactaacttt aaaaacaatt cctttgggca tagcttatgc aatttggggc ggattaggaa
     9061 ttgtattgac agcaataatt tcagtagtta tttttaaaca gaatctggat ttgcccgcta
     9121 tacttggcat tgcattaata gtatcaggtg tatttattat gaactttttt tccaaaacga
     9181 atgcccatta actattaata caatgcaaat atgcttttaa ttcattaagc ttttactttc
     9241 actactctta ctttccaagc atctggacca ttttcaatat attcccaacg gaatggctct
     9301 ttgctttctg cttccatttg gtaatacaaa ggttttggat cgtggtcgtt gataaattca
     9361 aaggcttcgc ctacttttat atcggcaaat gctttgtaaa acatttcatg tctttcagct
     9421 ggtggaaaag gacggatatc catttcattc actactgaaa aaccatcatc gcctaatcca
     9481 cttttctcca cttttttgat atgaataata tattcatcag gaacttgtct tttgtaagtc
     9541 caagtatatt gtccttcaaa tttatttttg aaaatttgtt gaatttcttc agggtcaata
     9601 gatgaaatta attccatagt cgtatctggt tccatcatac catagcgatg aaatacgatt
     9661 tgtttccatt cttttggtgc tgcttttctt aaatctaata aagtagaaat atctttcata
     9721 tctctttctt cagatgcagc tgtacgtgta actttcactt tccattctct gccacctcgg
     9781 tttaaatata cccaatctac cacatctcca taaatagatt tgaattcata aaataatgga
     9841 atagggtcgt ggtcattgat aaatacaaag ctttcagcga caggtaagtt tttaaaaagc
     9901 tctactaaaa attcatgtct ttggataggt gcaacaactc taacgtccaa ttctccgtgt
     9961 tcattttctc ttatttcgct cataatattt taatttaaga taattttgtc cgaaattaat
    10021 ataaatattg atattaagct ccaaaaatga agttaaataa gaattaaatg atagttgatt
    10081 gttatactaa atttcataat acataaaaac aagtaggaat tactattttg cttttagatg
    10141 tgtagttgga gcatacttac aacctgatac acattctcct ttgggattaa ttaaaaacca
    10201 attttcgtct gtttttactc ttgctaaacc ttgagcaaaa ggttcagtct cagaatagat
    10261 taaaggaata ataactttgc ctgttttatc aataaatccc cattttccac ttgaataatc
    10321 accctttcgc accattacta agccctcaga gaaatttcca gcatagtcat agtctgaagc
    10381 aattacttct tttcctgttt tgtcaataaa actatattta taattattct gagctattgc
    10441 tgcaataccc tccgaaaaat cacttgcatc ttcatattgc agatgaatga tctctgcacc
    10501 tgttttgtct atatatccat atttatcatt tatggaaaca cttgctaaac cttcagaaat
    10561 tttacctgca taatcatatt ttaatggaat aatttctttt ccagttttat ctataaagcc
    10621 aaatttttct acccccgatg ttcctgagct aacgacagcc attccttctg aaaaatcgcc
    10681 tacactatta tattttggag ctacaagctc tttccctgtc ttatctatga aaccccagtt
    10741 ttcatttaat tgtatttttg ctaaaccttc agagaaatta cctgcatctt cgtattggat
    10801 aggaataatt accttccctg ttttatcaat aaatcctgac aatgcatctc caagaatacc
    10861 ttcaactacc attgctaaac cttcagagaa tttctcagcc ttactatatt ttagaggaat
    10921 aacttctgtg cctgttttat ttataaaacc acatttaggc tcttctaatc cttcaaaatc
    10981 tccagtcatt accattgcaa gaccttcaga aaaatcaccc acaaaatcat acttcaatgg
    11041 tgtaactttt ttgcctgtct gatcaacaaa gccatattta taatctaatt ttacccaagc
    11101 aagtccatca tagaatttac ctacctcatc atattgattg ctccaattgc tagtggattg
    11161 tgccttgctt tgaaaaataa ataataagga aaagaacatt aagatacaaa attttgtttt
    11221 catagttttg atttttataa tttaaaatag ttttgtttta tcaatagttt tatttcgttt
    11281 aaaaatgaat gaaacgatat caattttttt tagttcaaaa aatataccat tttcaaacaa
    11341 atatagaatt ctttagataa actttcctaa ttaaaaagga attttttaat aaaatcaaat
    11401 gctttcattt taatatatga agaaaattat taaaaaatta ttggaaattg tttcaataca
    11461 aactcaataa aattagtact tctaagacct tatagcctac aacactaaaa attgaataat
    11521 tttagttatc tttaaattga ttgttatatg acttcggtcg catagttttt attatttagt
    11581 acttcattaa aatagtattg cttataatta tcttgaatgt ttatacaaca atttccaata
    11641 ccacattcat tacaaaatca tattgtaaac attagctttt ataaattaac gaaagatgaa
    11701 atgctaagaa gaaaaatatt gcctgataat attacttcct tatttgtgtt tttaaatcct
    11761 actatccact tgaaagaatg tttctctaat agtattatta actatagagt ggcttatgct
    11821 ggtccatatt caaaacctag actatatgaa gtagaagaag aaattcgttt tattaaaatt
    11881 gattttttcc cttggggagc cttttcagct tttggattgg aacaatataa attacacaat
    11941 acaaccattg atgcatataa attatttcca aaacttaaat tatattctaa accgcttgag
    12001 gcatctttgg acaatataga aacctgtatt gctattttag aacaatttat gtttgaagat
    12061 tgccatgaat accttacacc cactgatgaa aaaattatta ccgcttgtaa tttaattaag
    12121 agcaaggata agaatatccc cattaaagaa ttatgcacat tggtaaatat gtctcaaaat
    12181 tctttttgta atcattttac taagataatt ggtatttcgc caaaattatt tggacaaatt
    12241 gcacgttata gtaaagctaa aaaaataatt cattctacta ttgatattaa ttggaatgaa
    12301 attgctttcc aatttggata ttttgatcaa agtcatttta ttaaagaata caagcgattc
    12361 tctggtgcta ttccaaaaga atataaagca tggaagaatt tctataatta aagaaaaaga
    12421 caaatttatc tttttatgtg attttttact attttctaat aggtattata aataatttta
    12481 aattgattta taaaatttaa aacaaacggc tatgaaaaag gtaattattt attttagttt
    12541 aagtgctatc ttaatctttg gatgtaaaaa agataagata tcaaatacac caacgccacc
    12601 acccaaagga cagttagaga ttaaaattca agtaccagct aatactgatt tagaagtaaa
    12661 tggtggggaa gtattgtcag gagctacttt tgtggatata aaagataaaa acaaagtacc
    12721 cattcctgtt tttaaagacg atataacgcc agtttatgtt tttgataata aagacaattt
    12781 ggttatgatg ggttttgctg gagataatgc tagaatagta tcgtcagcaa caacagcaca
    12841 agtgcttttg tattggggac ttggacttcc ttatgcagat gtagcattta caaaatcatt
    12901 ttttgagcag tatgagggta ataagattgt aggtcaatgg gttcaaaaat ttgaacaaga
    12961 ttttatagcg gatccactta ttatttccaa gggtggtttt atagatggtt tgacagctac
    13021 ggtaaaagaa ctcagaaata aagatgtttt agatgctaat aatatgaaaa aatcagcaga
    13081 tataaaagta gatgctgcta atgtaaaaag tggattacga gtgcaggagg atggcttgag
    13141 ccagtattct gtcaataatt attaccgcag aagagctcac gcttacttat ataaaatgaa
    13201 ttatacggat atgaataaca gcaaacatac ccttattagt caatttggag aaaacacaaa
    13261 agctgagaat gattttccag tgagttctcg ttcaggagta acgagtgtta tgggtgaaat
    13321 aggtaaatgg gtagatggta gtgatatggg ttctatggta gccaaaacag atcctaacca
    13381 attacaattg gaggacaatg aaagtgaagc attgtataaa ttaagaattg taggtcctgg
    13441 tagtcttgca ggggcacaca tgagtggtaa tgaggctacg actgctgaac tttcaaaata
    13501 tttagaactc tcagcagaaa cctttctact agatttcctg attcctacac tatctttaat
    13561 tacttctgct gaaggtatga ctgctgattt tgattggagt aagaaagata tagatattgc
    13621 aattgatgca ataaaaagtt tttggaaaac tacccctgat gtgtatgaag agattaaaaa
    13681 aggaagttat tctaatgctc tgaaaaaggt aataacaagc ataggagcag atgtagcaaa
    13741 cacctatctg actgcaattt ttgatggtat tggcgatatg ggtttgaaaa acttatggtc
    13801 tggctctgtt acacatatca aaataagcaa ggttttggct gttgttgatt ttatattggg
    13861 tactaccgac ttggctctta caacgaataa tatcttatcc tccaaaggaa ttgaaagttt
    13921 cgatatcacg gctcgaactg gcaaggtaac actgctacca gtagaagcta caggtgtgtc
    13981 aggtagtttt attaaattag aagctataat taagaatatg gaattgcctc aaggaacaca
    14041 tccgtttttt gaatggagta cttcagggaa ttttggtgag ctttgggata atcaaggaca
    14101 tcgtagtgtt tcctttgaaa gtcaagacgc caagccacaa tattatgtaa aagcttcttc
    14161 taatcaactg agtgatggta ataattttga atacgtatat gtaaaagctt atataggcag
    14221 caatgtggtg ggtacagata ctactaaaat taatgtgaaa aaggtttctt ataaaatatt
    14281 gcctactggt gtaacaattt caggacaaaa agataaagca aatgagattc gtttatattt
    14341 ggtaagaagt gataataaaa aagatattgg acaagctgga gattatgatt ataaagtagt
    14401 ttgggaaaca gcaggaagct atggtaaact gaaaagcggg tttaataatt caacgacaat
    14461 tactacttat aattcggata atattactta ctcctgtacc gatgaagata caaaagaagc
    14521 acaggaagaa atcagagcaa aggtatatat gaaacaaaaa acagctcccg acgatgaata
    14581 ttttttagca agtgatacaa agggaattgt gaaaataggt aatgacgaga ataagaagat
    14641 tatccatata ccaatgaccg tttttattta tcagtttcca gatcaagata actggtgcgg
    14701 acaatatcca atggcaattt ttcccgtaga agatagtgca aaacattatc aggttgtttt
    14761 ttataatttt ccaccaaatt atatattaga tgggcgaatt atagaaaaaa taacttatag
    14821 ctgggcaaaa gggcaaagac caaataatga gtataatcct tttcatggag agaaccctcc
    14881 tttagttcat aatggacagt attatatatc gtttgcatgg actggtggta actgtgattg
    14941 gattaatact ttacctagtg ataatggagg agtgatggtt gaagtaacat attattatta
    15001 gagctaaaag acagcacgca gttatactat ttattctatg aatagaaata ttgttaagtt
    15061 gttatttttg taatttaact taagtaataa gcctcgtttt ctattgatga aaaaagtagg
    15121 aattaaatga ttaatgcttc gaatattgaa tttaagattt atggtttctt tgagccagtt
    15181 ccttacgcac tcgacttaaa ctttctggag taatacccaa ataagaagca accatcaatt
    15241 gtggaacacg taataaaata tcaggataga ttctagtaaa atgtaagtaa cgttcttcgg
    15301 cacttgcact caacaattga ttgattcggt tttgtaactg acggatatgg ttgtgtagta
    15361 gtttattatt gaattccata aacgaaacgt cttttttggc taaatctaac ataaaagatt
    15421 cgtctaaaat gatggcttca gtatcttcta atgcttgaat aaaataagct gacggctcat
    15481 taaagaaaat acttgctctg tctgaaacaa accaaccttc aggtgcaaat tgtaagagat
    15541 gctctttgcc tttatcatca atagaataat aacgaagtaa accattttct acaaaaagcg
    15601 tttgctgaca agcttctcca gcacgtaacc agaaatctcc ttttttaata tgcacatttt
    15661 tacaccgatt aagtagtgcc tccattaacg taggctctaa agtactattg gagctaagaa
    15721 agtctgcaaa gcgtatttct atcataggta atttaaaatt tgtcttatgg cttaaattta
    15781 agataattat tgagtttttt atataaaagc tatcagctat tgtaggaacg aattgtttta
    15841 atttgtattc ctgaatatga aatttgttta cactataata aataatagaa aacttcgcaa
    15901 aaatcaaatt acaataaata ataatagcct ttgttgttgt ggtaaattat taatgtttta
    15961 ctttttaatt aatattatta tattgtgtta taagaagaat attgaaaaat tattaaaatt
    16021 gtgttttcat ggctaactta ttagatttta ccgacaaata ccaactctcc aaaaccctgc
    16081 gttttgaact caaacccatt ggcagaacct tagagcatat tcaaaccaaa ggtttgttac
    16141 ttcaagatgc taacagagca aaaagctatc aagaagtaaa gaaactgata gaccgttacc
    16201 accagcattt tatagaacta gctcttagtc ctttaaaatt gacccaatta gaagatttac
    16261 agaatttata ttttgcccct gctgaggcta aaaaagaaga tgactttaaa aagaaatttg
    16321 aaaaaattca ggaggaacta agaaaagaaa ttgttactgc atttaagact ggagaatcca
    16381 acgagatttt taaagttctg gataaaaaag aactcattac aaagttaatg gtagattggt
    16441 tggagcagaa agaagataaa gataaagttg aggagttcaa aacctttacc acctatttta
    16501 ccggctttca tgaaaaccga aaaaacatgt acaccgataa ggcacagagt accgctattg
    16561 cttaccgttt ggtacatgaa aatttgccta agtttttgga taatataaag gtgtttgaaa
    16621 aactgaaagc agtaccggaa ctgtacgaaa agtgcagtgt actctataaa gagattgaag
    16681 aatatctaaa catacgctat atagatgaag cttttgagtt gggctattac aatatggttt
    16741 taactcaaaa acaaatagag gtgtataacc tcattattgg aggacgaaca cctaaagaaa
    16801 atgaaaagaa aattcaaggt ttaaacgaat acatcaatct ttataaccaa aaacaagcgg
    16861 acaaaaaaaa tagaattccg aaaattaaac cgctctacaa aatgattttg agcgataggg
    16921 aaagtgtttc atttttagct gagaaatttg ataatagtca agaagtactc aatgccataa
    16981 aggagtatta tcacgccaat ataaaatctt atcaatcctc tgaaaaagat gatactgaaa
    17041 atgtattgga acgagtaaaa gacttattgg caaacctaag tagtaataat cttttccaaa
    17101 tttatatccg caacggaaaa gccatcaccg atatttcaca agctctattt ggtgatttta
    17161 gtttgataaa agatgctttg aaagctaatt ttgaaaaaac acttcctgaa ccaaaaggta
    17221 aatctaaaga caccagacca gagaaaattg aaaaatacct aaaacaagat tatttctcta
    17281 ttgcagaaat agaaacagcc ctctggaatt atcgtgctga aaatgattta ctcaaagatt
    17341 tgaaggaaga agaacatctt atagcaagct attttaatag ccatttcaaa actacagtca
    17401 atgacaagga atatgattta gttgctaatg taatggctaa atacagttgt atcaaagggc
    17461 ttttggaaca ggattatccc gaagacaaaa aactaaatca agagacaaag gaaattgatg
    17521 aaattaaagc cttcttagat gctttgatgt tggttttgca ctttgtgaaa cccttgatgc
    17581 ttcctaacg
//
LOCUS       DAOQCW010000044        17366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1166_length_17366_cov_5.519554, whole genome shotgun sequence.
ACCESSION   DAOQCW010000044 DAOQCW010000000
VERSION     DAOQCW010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 17366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17366
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1166_length_17366_cov_5.519554"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="PLS16_05470"
     CDS             complement(<1..1006)
                     /locus_tag="PLS16_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60563.1"
                     /translation="MQQSFYTNKYSILLVYIVLVLLIYSQTFHFDFALDDDLIKEGIE
                     GKIIQFTDIFDVFKQRYNRVDYRPISMLTFALEYFFVGTINPKIAHIVNVIVFVLILF
                     STHYLFQLLFKGKYKLETFFMVCIFAVHPLVVEVVANIKSRDGLLSMLFAIWSLYFFL
                     KASFSNYKLKFLFYSWLFFIIGTFAKIDILGVLLFIVGFNFIKFNKRDWFWGIGIFVF
                     FLITYSIFRGTLVNYYLPIDTSVDSKLAITTFTENPVADLTGFFISIYTYLQVTFIYI
                     GKILLPIKLCYYYGYNYYQLDTNFSLKLLVLFLMFVSIIIGVIFLSKRNGFILIAGIG
                     F"
     gene            complement(1056..2246)
                     /locus_tag="PLS16_05475"
     CDS             complement(1056..2246)
                     /locus_tag="PLS16_05475"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNJ60564.1"
                     /translation="MNHDYNYYNTAIKNEALPLAFVDVDFLDKNIKAIAERAGNKNIR
                     VASKSIRNIWTLKRIFEANKKYQGVMSFTGAEALFLIEQGFDDILLGYPIIDKNQIRN
                     ICKLTKTGKKIILMTDCISHASLINSIAEEEQVTQPICIDVDMSSDFLGLHFGVYRSS
                     LISVRDAKTYITQLLNFKNVKLTALMGYEAQIAGVGDTNPANGIKNFAIKFLKKKSIP
                     EYTQRRKEVIEYIKQKCTTLEIINAGGTGSMEWNKVEDGVTEITVGSGFFSPVLFDYY
                     DNFKHLPAAGFALPIVRQPKDRMFTCLGGGYIASGSVGIDKQPKPYLPEGVQITENEG
                     CGEVQTPFVYNGKQSLKIGDAIFFRHSKAGELCERFNELYLIKEGSVVEKTPTYRGMG
                     KCFL"
     gene            2372..5842
                     /locus_tag="PLS16_05480"
     CDS             2372..5842
                     /locus_tag="PLS16_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60565.1"
                     /translation="MAIIIVGYAYGQVFKDASQLSNLRIKYFSSTANDFSLDTNFVFP
                     NSIVIRNINGILLSPETDYHYDMNTGNILFNSSITDSFYVTYRIFNTNLPTNFAHKKY
                     QSSYYIDSLDAFFKQYQKYQPKQINQIDFLDFGGLQYSGNFSRGVQFGSNQDLSLNSN
                     LDLRLSGKIGNDIEIIAALTDQNIPLQPDGTTQQLQDFDKIYIQINKEPHRITAGDFN
                     QKSSNNSYFLRFSKQLQGIGYANSFKFKKNMQLNTNTAFAVARGKFARNTFFGQEGNQ
                     GPYRLLGNNNESYIIILAGSEKVYINGNLLTRGETNDYIIDYNSGEIIFMPRFLITKD
                     SRIVVEFEYSDKNYFRTTIHNAIDFKYKNLKIYTEVYSEQDAKNKPILNDLNDSAKTY
                     LASIGNNISNAFFDGFKPTTYDETTIQYRLIDTTIGLNHYDSVFIYAPTNSGNLYKVN
                     FSFVGTNKGNYKTSKNSNNLRVYEWIPPIDGIPQGAYEPVILLITPKTQLMTVSGLQY
                     DYKNMYIKSEFAFSNKDMNTFSDIDNKASKGYATNLELGRNDTIKNNNQLKTTIKYEF
                     KQANFRQIEPYRNTEFTRDWNLNDINSTFNEHLFGFQETYNNSKINLKASYRFTSLIR
                     QKNYQGFESQIPISWNYKNLEFNADFKHLSTKDTTLSTSFFRPNIQIAYAIQKAKGLK
                     FGINYLQENNHIQNIIADTLSNNSFKFNIAGLNISMPDSNKWSFNLDYKFRNDFNGLN
                     NQMHIQSFANLIDFKGSANAIKNQHLNWSFTYRDLKVIDSVLAKTPSERTYLGRIEYG
                     NRFFKTALRSNFIYELGGGQERIRQFTYLQVQSGQGYYKWIDENQNGIKELNEFVQSQ
                     FSDSAQYIKVYTDLNEYIRAYTTSYTQTINLTPKAIWFNESGIKKFISKFQIVSFLNI
                     SRKTLKDKHVKPFNPFIMNSKDSNIVNLNSNIRNTIAFNTGDPTYYLAYSNQILNEKL
                     LLVNGYDTRKKIEHLIDGYYNIKTAYTLNYKLLFSKQLYISDFFSNNNLHLNIYGVEP
                     KFSYNFKTMIRTSISYGFLYKQNNPQYGNEKMLSNKLETEFRYSKAGKNTLTTKFSLI
                     LVNYNSETASTKSYTILEGLQKGKNYIWNMEYSRFIGSNLELLLVYEGRKTGTTKVVN
                     TGRAQLRAIF"
     gene            6068..6616
                     /locus_tag="PLS16_05485"
     CDS             6068..6616
                     /locus_tag="PLS16_05485"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNJ60566.1"
                     /translation="MKLKASKLLLIIAFTIFSTQNIKASNPFFDVLGKLCLENKIDLE
                     KIKNFFSDKNINLNEALDPQLYINIFNWRNAPYRLGGKTQKGIDCSNYVFQLNSECQS
                     DYATSYQLAKITNYIDPSELQEGDLVFFNVNGGGISHVGIYLQDGKFTHSSSSNGVII
                     SSMDEPYWSKRFCRAGRITSNK"
     gene            6619..6963
                     /gene="arsC"
                     /locus_tag="PLS16_05490"
     CDS             6619..6963
                     /gene="arsC"
                     /locus_tag="PLS16_05490"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00014.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HNJ60567.1"
                     /translation="MIEIYHNNRCSKSRCTLDILNDKAVEFKVVEYLKEAPSIETIQS
                     LLKKLNIPAHELIRKSEAIYIEKYKGKNLSETEWIQAMHENPILIERPIVINGDKAII
                     ARPPEKALELFS"
     gene            7099..9951
                     /gene="gcvP"
                     /locus_tag="PLS16_05495"
     CDS             7099..9951
                     /gene="gcvP"
                     /locus_tag="PLS16_05495"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HNJ60568.1"
                     /translation="MQIHTFRERHIGTSAKETEEMLSIIGVPSVDALIEKTIPQHIKI
                     NHEITVGQAMSEYEYLSFIRAKATENKIYNNFIGMGYYGTLTPTVILRNIFENPGWYT
                     QYTPYQAEISQGRLEALLNYQTMVSELTGMPIANASLLDEATAAAESMHLLHEVRSKV
                     KNAPSANKIFIDQNTFPQTIDVVKGRALPLNIEVVVGDYTNFTPTQEYFSAIVQYPAA
                     DGAVNNFTEFFQKCKASNIYTTVIADILSLAILTPPAEFGADIVVGSTQRFGVPMGFG
                     GPHAAYFACKEEFVRLMPGRLIGVSVDKHGNRALRMALQTREQHIKREKATSNICTAQ
                     ALLAIMAGMYAVYHGNDGIKNIAQEIHNKANALAQTLTKLGFSIQHQHYFDTISVAIE
                     DANKLKAIALNHQTNLWYNNNTIQITIDETTTNQQLQLVADIFANYKGIASSEVKVVE
                     ASAIPASLQRKLDCLTHPIFNKYHSEHEMLRYIKRLENKDLSLCHSMISLGSCTMKLN
                     ATSEMMPLSLPEFANIHPFVPKDQAQGYLSIIKELENYLAQITGFAACSLQPNSGAQG
                     EYAGLMVIRAYHVAHNQTQRTTAIIPASAHGTNPASAVMAGMDVVVVKTDESGNIDVE
                     DLKLKVEKHKDTLSCLMVTYPSTHGVFEPTIKDICQIIHDNGGLVYMDGANMNAQVGL
                     TSPATIGADVNHINLHKTFAIPHGGGGPGMGPICVNDKLAPYLPKHAIVETGGTEGIA
                     AISAAPYGSASILLISYAYIKMLGQNGIKDATKYAILNANYMKKRLETHFPILYTGEN
                     GSVAHEFIIDLRPLKKDSNIDAVDVAKRLMDYNFHAPTVAFPVPGTLMIEPTESENKD
                     ELDRFCDAMISIRQEIEDIINGKTTKDDNPIINAPHTMFEATKTNWEHPYSREQAAFP
                     LQYVKENKFWASVGKVDNTYGDRNLVCTCPPMEDYM"
     gene            10333..11769
                     /locus_tag="PLS16_05500"
     CDS             10333..11769
                     /locus_tag="PLS16_05500"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNJ60569.1"
                     /translation="MKFYLFIFITILTGQAVFSTTKNFDITIQVKGIKNDFALLAYHY
                     ETSRFVQDTIQFNDKGIASIKGNKNYPDGVYIIAFPTLDFKFFDLIINKETKFSLTTD
                     TIDLPMNMKVSNSIENEALYNNLKYSIPLGFKIDSLKKLLKDLPDSSKTYKNIIEKIE
                     TQTKLTNEHRASVIEKYPNTFYASLLKMMQAPEPIIPKSYYTKNKEWKDTVTTVSYIK
                     EHYFDIIDWSDSAYVRSPIFKQFINQYFDLYIFPLPDSIIPPLDNLIAKAAQGSPLMY
                     KYVFNIVYDKYSKSQIMGYDRIFVHLVDKYLGNGKTPWIEGDQLKKIKDYAEDIRPVL
                     IGNPAADFTFKDSNYVDIKFHALLDKAEYTILVFWNSDCGHCQHDIPLINAKYDSLKT
                     LGAQVVSISTEQTDSTFRAFAAKNCNLNWITGWDPNGTSAFRREYFINATPKVFIIDR
                     KEQKIRAKNLPIADLYGYLEFLKNKEKE"
     gene            11850..12746
                     /locus_tag="PLS16_05505"
     CDS             11850..12746
                     /locus_tag="PLS16_05505"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNJ60570.1"
                     /translation="MTAINLSEFKALVSLLDDDDNEVFTHVSNKLVSFGKEGLSLLES
                     AWEAEENIVIQEKLESLIKKIQYDNIKDRLKQWLESDEQDLLEAALIVNTIEYPEIDE
                     LAIIDKINSIAKSVWIELNAALSPLEELQVMNQVFYQLHGFFSNNDKNTFKQIPDLGN
                     LNKILETKNGNSISLGILFLIIAQKNDLPIYGVNLPYHFIMCYARKHLSKEILDENTD
                     EKQVMFYINPINKGIAFSRMEITNYLKQIKLTSKKNYYSPCNNKAIIKALIFNQIAYY
                     DKESDKAKFLKELFFMIKSPIA"
     gene            complement(12732..13124)
                     /locus_tag="PLS16_05510"
     CDS             complement(12732..13124)
                     /locus_tag="PLS16_05510"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HNJ60571.1"
                     /translation="MSKKIPFSVERTYKSSPSILYSFLTTPSGLVQWFADYVDLDPNG
                     EIFTFEWNGDEQRAIVLAYEDDEYVKLQWEDEEDEKAYFEYKIKVTDITKETILTVTD
                     YAEKEDIPSAKELWLSQLNDLAKALGNG"
     gene            complement(13198..>14154)
                     /locus_tag="PLS16_05515"
     CDS             complement(13198..>14154)
                     /locus_tag="PLS16_05515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNJ60572.1"
                     /translation="IAEIQPSFFEITVAMAFYYFAKQKVDIAIIETGLGGRLDSTNII
                     QPLLSIITNISYDHQSILGNTLAEITNEKAGIIKCKTPAIIGRNQPEIRNVFEAKAKN
                     ENAPLFFADELIQVDNYTLKDGLLNIDFVLRNTKEKEMICTDLNAVYQIENMKTVFAA
                     MLVYNQFYEAIPMSKIKAGLLNVQQQTNFGGRWQVISTEPKIVVDVGHNEDGIVKIIE
                     QLKLEKYEHLHLIYSAVKDKDVDKIIRLLPANATYYLTEAKLERKMPFEILQDKFNAY
                     NLSNYGFENATLALQAAKKNANKLDLILVVGSFFILEDILQY"
     assembly_gap    14155..14254
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14321..15250
                     /locus_tag="PLS16_05520"
     CDS             14321..15250
                     /locus_tag="PLS16_05520"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNJ60573.1"
                     /translation="MKVFRGLDQLPKFKNAVLTIGTYDGVHYGHQYIINRINKIAQEI
                     GGESVLLTFYPHPRLILHPNDNTLKLITTIDEKIEILESLGLDNLVLIPFSPEFAQQS
                     AEEYIEKVLIRNFNPKYICVGYDHKFGKNRIGDYQLLEQLKEKYGYQLEEIPAQTIDE
                     ISVSSTKVRKALLEGDIATANHFLAYPFRIKSKVIHGDKIGKVLGFPTANIEVADEHK
                     LIPASGVYAVWVKVDEHQFKGALSIGYRETVFDNSELTIEVFILDFNKNIYDKSIELI
                     FVDYIRPQIKYENWDLLKEQIAKDVEQINALMD"
     gene            complement(15266..15907)
                     /locus_tag="PLS16_05525"
     CDS             complement(15266..15907)
                     /locus_tag="PLS16_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNJ60574.1"
                     /translation="MYKIFIRNKKVFLTNNPANIQEILTQSNIILLNYDENNDIQQIV
                     NGFILNDKSKSDVIIFCKNIEILKNDFFACFEVVEAAGGVVFNPANEILLIHRRGSWD
                     LPKGKIDDGETISQAALREVEEETGIKNIEILKPIELKYNQTQITYHYYELKGKQCIK
                     LSYWYIMKINESQNLIPQTEEDIEQAVWTKTEIIPSLYGNMYESVIDVIESVI"
     gene            15918..16556
                     /gene="pyrE"
                     /locus_tag="PLS16_05530"
     CDS             15918..16556
                     /gene="pyrE"
                     /locus_tag="PLS16_05530"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNJ60575.1"
                     /translation="MENLEFKQKLAEYLLQIKAIHFNLQNPYTWASGWKSPMYCDNRL
                     ILSYPDVRNFVRDGLIQMIEAYFPEADCIAGVATAGIPHATMIADHLNLPLIYVRSKP
                     KEHGRENLIEGELKHGMRVVVIEDTISTGGSSLQAVEALRKQGAEVIGMCAIYKYGFD
                     VARKRFEESGVTLKTLTNYDYVLRAGIESKYIDDVALVELKKWVSQPDAWNK"
     gene            16562..17182
                     /locus_tag="PLS16_05535"
     CDS             16562..17182
                     /locus_tag="PLS16_05535"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HNJ60576.1"
                     /translation="MLLFSISFTSWQTYAVPIIGAFIGWITNYIAIKMLFHPRKPIKI
                     LFVTFHGIFPKNKARIANKLGTVVQRDLINFSDIKDRFKDPDALSNFKEEIAIRIDDA
                     IRDRLAKTPVLNTIVPEPFIQGIHKTIVKEIETNLPHVIDSSITKIEQKLDIQELVRN
                     KVANFSDEKLENLLLDITAREFKFIELIGAVLGFLIGIIQLLIQAI"
BASE COUNT         6458 a         2620 c         2407 g         5781 t
ORIGIN      
        1 aaaaccctat tccagcaatt aagataaaac catttctttt ggataaaaat ataacaccaa
       61 ttataataga aacaaacatt aaaaataaaa ctaaaagttt taaagaaaaa ttagtatcta
      121 attggtaata gttgtatcca taataataac acagctttat aggtaataaa atctttccaa
      181 tatagatgaa tgtaacttgc aaatatgtat atatactaat aaaaaaacca gttaaatcag
      241 ctactggatt ttcggtaaat gtagtaattg caagcttact atcaacagaa gtatctattg
      301 gtaaataata attaacgaga gttcctctga atatggaata tgtaattaaa aaaaatacaa
      361 aaattccgat tccccaaaac caatctcttt tattgaattt aataaaatta aaaccaacta
      421 taaataataa aactcctaag atatcaatct ttgcaaaagt tccaattata aaaaatagcc
      481 aactataaaa taggaattta agtttatagt tactaaaact tgcttttaaa aaaaaataca
      541 aactccatat agcaaataac atactcaaca atccgtctct cgattttata ttagcaacta
      601 cttcaacaac taaagggtgt accgcaaaaa tacacaccat aaaaaaagtt tctaatttat
      661 attttccttt aaaaagtaat tgaaaaagat aatgcgttga aaaaagtatc aaaacaaaaa
      721 caattacatt aactatatgt gctattttag gatttattgt acctacaaaa aaatactcca
      781 atgcaaatgt aagcatactg attggccgat aatctactct attatatcgc tgcttgaaaa
      841 catcaaatat gtccgtaaat tgaattattt ttccttcaat tccttcttta attaaatcat
      901 catctagggc aaaatcaaaa tgaaaagttt gtgaataaat caacaatacc aatactatat
      961 atactaataa aatactgtat ttatttgtat agaatgattg ttgcattgaa taaaactact
     1021 aaaagaataa tgaaattaaa tattatttta aatctttaca aaaagcattt gcccattcct
     1081 cgataagttg gtgttttttc aacaactgaa ccttctttaa ttaaatataa ttcattaaat
     1141 cgctcacaca actcacctgc tttactatgt ctaaaaaata ttgcatctcc aatttttaac
     1201 gattgctttc cattataaac aaatggtgtc tgaacttcac cgcaaccttc attttctgta
     1261 atttgtacac cttctggcaa atatggtttt ggctgtttat ctatacccac acttccactg
     1321 gcaatataac cgccacctaa acaagtaaac attctatctt ttggctgtct aacaatgggg
     1381 agtgcaaatc cagctgctgg taaatgctta aaattatcat agtaatcaaa taatactggt
     1441 gagaaaaaac ctgaacctac tgtaatttct gttacgccat cttcaacttt attccactcc
     1501 atacttccag ttccacctgc atttataatc tctaaggttg tacatttttg tttaatgtat
     1561 tcaatgactt cttttcttct ttgtgtatat tcaggtatag attttttctt caaaaattta
     1621 attgcaaaat ttttaatgcc attggctggg ttcgtatctc caactcctgc aatctgtgct
     1681 tcataaccca ttaatgctgt taattttaca ttcttaaaat tcaataattg agtgatatat
     1741 gtttttgcat cacgaacact aattagagaa gaacgataaa cgccaaaatg taagcctaaa
     1801 aaatctgaag acatatctac atcaatacaa attggttgcg taacttgttc ttcttcagca
     1861 atggaattta tcaaactcgc atgacttata caatctgtca ttaagataat tttctttcca
     1921 gttttagtga gtttacaaat atttctaatc tgatttttat ctataattgg atagcctaac
     1981 aaaatatcat caaatccttg ctctattaaa aacaaagctt ctgctcctgt aaaagacatg
     2041 acaccttgat atttcttatt tgcttcaaaa atgcgtttta atgtccaaat atttcgtata
     2101 gattttgatg ctacacgaat gtttttatta cccgcacgtt cagctattgc tttaatattt
     2161 ttatctaaga aatcaacatc aacaaaagct aatggcaaag cctcattttt aatagctgta
     2221 ttgtaataat tataatcgtg attcatttgg tatttatttt taatgttcac aaatataata
     2281 agcttcatct aatattctat aaatagcaag aaacaataga aaacttttat ctttatcctt
     2341 gatgtcttta atcaaaaaca tattatttat attggctata ataatagttg ggtatgctta
     2401 tggtcaagta tttaaagatg catcacaatt atctaattta agaataaaat atttttcaag
     2461 cacagctaat gattttagct tagatacaaa ttttgtattc ccaaatagta tagtaatcag
     2521 aaatataaat ggcatcttac tttcccctga aactgattat cattatgata tgaatactgg
     2581 aaatatactt ttcaattcga gcattacaga ttcattttat gttacttata gaatattcaa
     2641 cactaattta ccaacaaatt ttgcccacaa aaaataccaa tcttcttatt atatcgattc
     2701 cttagatgct ttttttaaac aataccaaaa atatcaacct aaacaaatta atcaaattga
     2761 tttcttagat tttggaggtt tgcaatatag cgggaacttt tctcgtggtg ttcaatttgg
     2821 ttctaatcaa gatttaagtt taaattccaa tttagaccta agattatctg ggaaaatagg
     2881 caatgatata gaaataattg ctgcacttac cgaccaaaat attcctttac aaccagatgg
     2941 tacaacgcaa caattgcaag atttcgacaa gatttatata caaatcaata aagagccaca
     3001 ccgtataacc gctggtgatt tcaatcaaaa atctagtaat aattcatatt ttttacgctt
     3061 ttctaagcaa ttgcaaggta taggctatgc aaattctttc aaatttaaga aaaatatgca
     3121 actcaatacc aacactgctt ttgcagttgc tagaggaaaa tttgccagaa atacattttt
     3181 cggtcaagaa ggcaatcaag gaccgtatcg attgttgggg aataacaacg aaagttatat
     3241 cattatctta gcaggaagtg aaaaagtata tatcaatgga aatttactaa ctagaggcga
     3301 aactaatgat tatatcattg attataattc aggtgagatt atttttatgc ctagattttt
     3361 aattactaaa gactccagaa ttgttgtaga atttgaatac tctgacaaaa attattttag
     3421 aactactata cataacgcta ttgattttaa atataaaaat ttaaaaattt acaccgaagt
     3481 ttatagcgaa caagatgcta aaaataagcc tatcttaaat gatttaaacg acagtgccaa
     3541 aacatattta gcaagtatag gaaacaatat cagcaatgct ttttttgatg gctttaaacc
     3601 tactacctat gatgaaacca cgattcaata tagactaata gatacaacaa ttggattaaa
     3661 tcattatgat tcagttttta tttatgctcc cactaattct ggaaatttat ataaagtcaa
     3721 tttttcgttt gtaggcacaa ataaaggcaa ttacaaaacc tcaaaaaact ccaataactt
     3781 gcgtgtgtat gaatggatac ctccaataga tggaatacct caaggtgctt atgaacctgt
     3841 tattttatta atcacaccca aaacacaatt aatgacagtt tcaggtttac aatatgatta
     3901 taaaaatatg tatatcaaat ctgaatttgc cttttccaat aaggatatga atactttttc
     3961 agacatagac aacaaagcca gtaaaggtta tgcaactaat ttagaactag gaagaaatga
     4021 tactataaaa aacaacaacc aacttaaaac aactataaaa tatgaattta aacaagcaaa
     4081 ctttagacaa atagaaccat acagaaatac tgaatttaca agagattgga acttaaatga
     4141 tattaattct acattcaatg aacatctttt tggctttcaa gaaacttata ataattcaaa
     4201 aataaattta aaagcaagct atcgttttac atcgctcatc agacagaaaa attatcaagg
     4261 atttgaaagt caaattccaa tttcttggaa ttacaaaaac ttagaattta atgctgattt
     4321 taaacattta agtacaaaag atactacttt gtcaacttca ttttttagac ctaatattca
     4381 aattgcctat gcaattcaaa aagcaaaagg attaaaattt ggcattaatt atttacaaga
     4441 aaacaaccat attcaaaata taattgcgga cacattaagc aacaatagtt ttaaatttaa
     4501 tatagctgga ttaaatattt caatgccaga ctctaataaa tggagtttta atttagatta
     4561 taaatttaga aatgatttca atggcttaaa taatcaaatg catatacaat cttttgcaaa
     4621 tttgatagat tttaaaggaa gtgccaacgc cattaaaaat caacacctca attggagttt
     4681 tacttatcgt gatttaaaag taatagattc cgtattagct aaaacaccct cagaaagaac
     4741 ctacttagga agaattgaat acggaaatcg ctttttcaaa actgcattgc gtagtaactt
     4801 tatatatgaa ttgggtggtg gacaagaacg cattcgacaa tttacttatt tacaagttca
     4861 atcaggacaa ggttattaca aatggataga tgaaaaccaa aatggcatca aggagttgaa
     4921 tgagtttgtg caatcacaat ttagcgacag tgcacaatac atcaaagtat atactgactt
     4981 aaatgaatac atcagagcat acaccacttc atatactcaa acaattaatt taacacctaa
     5041 agctatctgg tttaatgaaa gtggaattaa gaaatttatt agtaaatttc aaatagtatc
     5101 cttcctaaac atcagcagaa aaactttaaa agacaaacat gttaagccat tcaatccatt
     5161 tataatgaat agtaaggata gtaatattgt aaacctaaat tcaaacatta gaaacactat
     5221 tgctttcaat acaggcgatc caacttatta cttagcttat agcaatcaaa tactcaatga
     5281 aaaattactt ttggtaaacg gttatgatac aagaaaaaaa attgaacacc taattgatgg
     5341 atattacaac ataaaaactg cttatactct aaattataaa ctccttttta gtaagcaatt
     5401 atatatttca gatttcttta gtaataacaa cttgcatttg aatatctatg gtgtagaacc
     5461 aaaattctct tataatttta aaactatgat tagaacttct atttcttatg gttttttata
     5521 taagcaaaac aatcctcaat atggaaacga aaaaatgcta tccaacaaat tagaaacaga
     5581 atttagatat agcaaagcag gaaaaaatac acttaccaca aaattctctt taattttagt
     5641 caattataat tctgaaacgg cttcaaccaa aagctatact attttagaag ggttgcaaaa
     5701 aggcaaaaac tatatttgga acatggaata ttcaagattt ataggttcaa acttagaatt
     5761 acttttagtt tatgaaggtc gaaaaactgg cacaacaaaa gttgtaaata caggcagagc
     5821 acaattaaga gcaatttttt aatagaaaac aatactatat tagttagata atatttcata
     5881 aactgtttat cttataatat ctaaaaaatc atttatattt cttaaaatat ggttaaaatc
     5941 agcaaaattt tcaattatag ctaactagat ttggttattt agtctttttt ttcttatatt
     6001 tgaaaactaa cgaatattaa ccacaaacca taaatcattt ttataactaa aaaaaactaa
     6061 ttatagaatg aaattaaaag caagtaagtt attattaatc atcgcattta ccatattttc
     6121 aacacaaaat ataaaagcaa gtaatccatt ttttgacgtt ctcggaaaat tgtgtttaga
     6181 aaataaaatt gaccttgaaa aaataaaaaa cttcttttct gataaaaaca tcaatttaaa
     6241 cgaagcatta gacccacaat tatatattaa catcttcaat tggagaaatg ctccatatcg
     6301 tttgggtggc aaaactcaaa aaggcattga ttgttccaat tatgtttttc aacttaactc
     6361 agaatgtcaa agtgattacg ccacatctta tcaattggca aaaattacta actatattga
     6421 cccttccgaa ttacaagaag gcgacttagt atttttcaat gtaaatggtg gtggcatttc
     6481 acatgttggt atttatttac aagatggtaa attcacgcac tcttcttctt ccaacggagt
     6541 tatcatcagt agtatggatg aaccttattg gtctaaaaga ttttgcagag ctggtagaat
     6601 cacatctaac aaataaatat gatagaaatt tatcacaata acagatgtag taaaagcaga
     6661 tgtactttag atatcctaaa tgataaagca gttgaattta aagttgtaga atatttaaaa
     6721 gaagctccaa gtatcgaaac gatacaatct ttattaaaaa aattaaatat tcctgctcac
     6781 gaattaataa gaaaaagcga agccatttat attgaaaaat ataaaggtaa aaatttaagt
     6841 gaaacagaat ggattcaagc gatgcatgaa aatccaattt tgattgaaag acctattgtt
     6901 attaatggcg ataaagctat aattgcacga cctccagaaa aagcattaga attattctcc
     6961 taattcaatt aacatcagct ttttttatat ttttctattt cttgatttta ttaaatgaac
     7021 aattttagtt actgttacta aaataattgg gtatatccat agcttaattt tatctttgcg
     7081 taactcaaaa aaacatttat gcaaattcat acttttagag aaagacacat cggaacttct
     7141 gcaaaagaaa cagaagaaat gttatcaatt attggcgttc catctgtaga tgcactaatc
     7201 gaaaaaacga taccacaaca cattaaaatc aatcacgaaa ttactgttgg acaagcaatg
     7261 tctgaatatg aatatttgag tttcataaga gcaaaagcaa ctgaaaataa aatatataat
     7321 aactttattg gaatgggtta ttatggtaca cttacaccaa ctgtaatttt aagaaatata
     7381 tttgaaaatc caggttggta cacacaatac acaccctatc aggcagaaat ttctcaagga
     7441 agattagaag cattattgaa ttaccaaaca atggtttccg aattgacagg aatgccaatt
     7501 gctaatgctt cactattaga tgaagctact gcagctgcag aatctatgca cttactacat
     7561 gaagtaagaa gtaaagtaaa aaatgcacca tctgccaata aaatttttat agaccaaaat
     7621 actttccctc aaacaattga tgttgtaaaa ggaagagctt tacctttaaa tatcgaagtc
     7681 gtagttggcg attataccaa cttcacgcct actcaagaat atttttctgc cattgtacaa
     7741 tatccagctg ccgatggtgc tgtaaataat tttactgaat tttttcaaaa atgcaaagct
     7801 tcaaatattt acacgacagt tattgcagac attttaagct tagccatatt aacacctcct
     7861 gcagaatttg gtgctgatat tgttgttggt tctactcaaa gatttggtgt acctatggga
     7921 ttcggaggtc cacatgctgc atattttgct tgtaaagaag aattcgtacg tttaatgcct
     7981 ggaagattaa ttggtgtttc tgttgataaa catggcaata gagctttgcg tatggcgctt
     8041 caaacaagag aacaacacat caaacgagaa aaagctacat caaatatatg tacagctcaa
     8101 gctctattgg ctattatggc tggaatgtat gcagtttatc atggaaatga tggtattaaa
     8161 aacatcgctc aagaaattca taataaagcc aatgcattag cacaaacgct tacaaaatta
     8221 ggttttagta ttcaacatca acattatttt gatactatta gtgttgccat tgaagatgct
     8281 aataaactaa aagcgatagc attaaatcat caaacaaatt tgtggtataa caacaatact
     8341 attcaaatta caattgatga aacaaccacc aatcaacaat tgcaattagt tgctgatatt
     8401 tttgccaatt ataaaggcat tgcttctagc gaagtaaaag ttgttgaagc ttcagcaatc
     8461 ccagcaagtt tgcaaagaaa attggactgt ttaacacacc caattttcaa taaatatcat
     8521 tcagaacatg aaatgctaag atatatcaag agattagaaa ataaagattt atcgctttgt
     8581 cattcaatga tatcattagg aagttgtact atgaaattga atgcaacatc agaaatgatg
     8641 cctttaagtt taccagaatt tgctaatatt catccatttg tacctaaaga ccaagcacaa
     8701 ggatatttaa gtattattaa agaattagaa aactatttag ctcaaattac aggatttgca
     8761 gcttgttcat tacaaccaaa ctcaggtgct caaggtgaat atgctggatt gatggttatt
     8821 agagcttatc acgtagctca taatcaaaca caaagaacaa ccgcaataat accagcatca
     8881 gctcacggaa ccaatcctgc gagtgcagta atggctggaa tggacgtagt tgtggtaaaa
     8941 actgatgaaa gtggcaatat agatgttgaa gatttaaaac taaaagtaga aaaacataaa
     9001 gatacacttt cttgtttaat ggtaacttat ccatcaacac atggtgtttt tgaacctact
     9061 ataaaagata tttgtcaaat tatacatgac aatggtggtt tagtttatat ggatggtgcc
     9121 aatatgaatg cacaagttgg gttaacttct cctgcaacaa taggtgcaga tgtaaaccac
     9181 atcaacttac ataaaacatt tgcaattcct catggtggtg gtggaccagg aatgggacca
     9241 atttgtgtga atgataaatt agctccctat ttacctaaac atgctattgt tgaaacaggc
     9301 ggaacagaag gtattgcggc aatcagtgcc gcaccttatg gtagtgcatc tattctatta
     9361 atatcttatg cctacattaa aatgttagga caaaatggta ttaaagatgc tacaaaatat
     9421 gctatcttga atgctaatta tatgaaaaaa agattagaaa cacattttcc aatcttatat
     9481 actggcgaaa atggtagtgt tgctcacgaa tttattattg atttaagacc attaaaaaaa
     9541 gattcaaata tcgatgcggt tgatgttgca aaacgtttaa tggattataa tttccacgct
     9601 ccaacggtag catttccagt gccaggcact ttaatgattg aaccaacaga aagtgaaaac
     9661 aaggacgagt tagaccgctt ttgcgatgct atgatttcta ttagacaaga aattgaagat
     9721 ataattaatg gaaaaaccac taaagatgac aacccaatta ttaatgcacc acacacgatg
     9781 tttgaggcga ctaaaaccaa ttgggaacat ccatatagta gagaacaagc tgcgttccca
     9841 ttacaatatg taaaggagaa taaattttgg gcttctgtag gaaaggtgga taatacctat
     9901 ggcgatagaa acttagtttg tacttgtcct ccaatggaag attatatgta ataatatcga
     9961 attttattgc taaaaaaatg cttatatatt aaatcaaaaa tataagacaa gcttttattg
    10021 ttatagactt atcgcatatt ggttttgctg agatttttag gaaagacaat aaaacggtaa
    10081 ctatagctgt aatgctgagc ctatgaatta aattggtgac tttttgtaat cattatggtc
    10141 tctaataaat gcttcaaagc aaactatagc catatttttc agtactatgc ctaattctta
    10201 aaaaaaacta tatccgtttc ctacatttca attattgatt aacaaaaatt aatactttaa
    10261 atacttacag ttctattgaa aagatacttt gtttctatac ttttggaata gattttgcaa
    10321 atactaattt atatgaaatt ttatctattt atttttataa ccattttaac tggacaagcc
    10381 gttttttcaa caactaaaaa ttttgacatt acgattcaag taaaaggtat aaaaaatgat
    10441 tttgctttat tagcttacca ctatgaaaca agtcgctttg ttcaagatac catacaattt
    10501 aatgataaag gaatcgcttc aattaaaggc aacaaaaact atccagatgg cgtttacatt
    10561 atcgccttcc ctactttaga ttttaagttc tttgatttaa ttatcaataa agaaacaaaa
    10621 tttagcttaa caacagatac tattgattta cctatgaata tgaaagtttc aaatagtatt
    10681 gaaaatgaag ctttgtataa taatttaaaa tattctatcc ctttaggctt taaaattgat
    10741 tctttaaaaa agctactaaa agacttgcct gattcttcaa aaacatataa aaacattatt
    10801 gaaaaaattg aaactcaaac aaaattaact aacgagcatc gtgcttctgt aattgaaaaa
    10861 tatccaaata ctttctatgc gtcgctatta aaaatgatgc aagcacctga gccaattatt
    10921 ccaaaatctt attatacaaa aaacaaagaa tggaaagata ccgtaacaac tgtatcttat
    10981 attaaagaac attattttga tataattgat tggtcggatt ctgcttatgt tcgtagtcct
    11041 attttcaaac aatttatcaa tcaatatttt gatttgtata tatttccatt gcctgattct
    11101 atcattccgc cattggataa tttgatagca aaagcagcac aaggttctcc attaatgtat
    11161 aaatacgtat ttaatattgt atatgataaa tattccaaga gtcaaattat gggatacgat
    11221 aggatttttg ttcatctggt tgataagtac ttaggtaatg gaaaaacacc ttggatagaa
    11281 ggcgatcaac ttaaaaaaat aaaagattat gcagaagaca ttcgacctgt attaattgga
    11341 aacccagcag cagattttac ctttaaagat tcaaattatg tggacatcaa attccatgct
    11401 ttattggaca aagctgaata tactattttg gtattttgga attcagattg tggacattgc
    11461 caacatgata ttccattaat taatgctaaa tatgattcat taaaaacatt gggtgcacaa
    11521 gtggtaagta tttcaacaga acaaacagac agtactttta gagcttttgc cgcaaaaaat
    11581 tgtaacttga attggataac aggttgggac ccaaatggca caagtgcttt cagaagagaa
    11641 tattttataa atgcaacacc taaggtattt atcattgata gaaaagaaca aaaaattaga
    11701 gctaaaaact tgcctattgc tgatttatac ggctatttgg aatttttgaa aaacaaggaa
    11761 aaagaataac caaaagactt ctttcaattc ttcacgaatg tttaaaagta tttttcactt
    11821 acattgaaaa tagaactata ttagtactta tgacagcaat taatttatct gaatttaaag
    11881 ccttagtctc cttattagac gatgatgaca atgaagtctt tacacatgtc agtaacaaat
    11941 tagtatcatt tggaaaagag ggattatctt tattagaatc agcttgggaa gctgaagaaa
    12001 acattgttat acaagaaaaa ctagaaagct taataaaaaa aatccaatac gacaatataa
    12061 aagatagatt aaaacaatgg ttagaaagtg atgaacaaga tttattggaa gcagcattaa
    12121 tagtaaatac aattgagtac ccagaaattg atgaattagc aattatcgat aaaattaatt
    12181 ccattgccaa atctgtttgg atagaattga atgcggcact ctctccactc gaagaattac
    12241 aagtgatgaa ccaagttttt tatcaattac atggtttttt cagtaataac gataaaaata
    12301 ctttcaaaca aatacctgat ttaggcaatt tgaataaaat attagagact aaaaatggca
    12361 attcaatttc tttaggcatt ttatttttaa ttattgctca aaaaaatgac ttgccaattt
    12421 atggtgttaa tcttccttat cattttatta tgtgttatgc tagaaagcat ctttctaaag
    12481 aaattttaga cgaaaatact gatgaaaaac aagtgatgtt ttatatcaat ccaataaata
    12541 aaggcattgc attttcaaga atggaaatta ccaattatct aaaacaaatt aaattaacat
    12601 ctaagaaaaa ttattattct ccctgcaata ataaagcaat tattaaagca ttaatcttta
    12661 accaaatcgc atattacgat aaggaaagtg ataaagctaa atttttaaag gagttatttt
    12721 ttatgataaa atcacccatt gcctaaggct tttgctaaat cattcaattg actaagccac
    12781 aattctttgg cactaggaat atcttctttt tcagcataat ccgttacagt taagattgtt
    12841 tcttttgtga tatccgtaac tttaattttg tattcaaaat acgctttttc atcttcttca
    12901 tcttcccatt gtaattttac atattcatca tcttcgtaag ccaaaacaat agctctctgc
    12961 tcatcgccat tccattcaaa tgtaaatatc tctccattgg gatctaaatc cacataatct
    13021 gcaaaccatt gaaccaatcc cgaaggtgtt gtcaaaaaac tatataaaat cgatggtgat
    13081 gatttgtaag ttcgctcaac tgaaaaaggt atttttttac tcataaactt gaattacccg
    13141 caattaagga taaatattta tcatttccaa atttaatttc attttttttt aaaaaaatta
    13201 atattgtaaa atatcttcta aaataaaaaa actaccgact actaaaatta agtctaattt
    13261 gtttgcattt tttttcgcag cttgcaaggc taaagttgcg ttctcaaatc cataattcga
    13321 taaattatag gcattaaatt tatcttgtaa tatttcaaat ggcatctttc tttctaattt
    13381 tgcttcagtc aaataataag tagcatttgc aggcaataaa cgtattatct tatcgacatc
    13441 tttatctttt actgcactat aaataaggtg taaatgctca tatttttcta gttttaattg
    13501 ttcaataatt ttgacaattc catcttcatt atgtccaaca tcaacaacaa ttttaggctc
    13561 agtagaaata acctgccaac gtccaccaaa atttgtttgt tgctgtacat ttagtagccc
    13621 agccttaatt ttactcattg gtattgcttc ataaaattga ttataaacta acattgctgc
    13681 aaaaactgtt ttcatatttt ctatttgata aacagcattc aaatccgtac aaatcatttc
    13741 tttttcttta gtgtttctaa gcacaaaatc aatattcaac aagccatctt ttagtgtata
    13801 attatcgact tgaattaatt catctgcaaa aaacaaaggt gcattttcat tcttagcttt
    13861 agcttcaaaa acatttctaa tttctggttg attcctacca ataatagcag gcgttttgca
    13921 ctttattata cctgcttttt catttgtaat ttcagctaaa gtattaccca aaatagattg
    13981 atgatcgtaa ctaatattag taataatact caataatggt tgaataatat tagtagaatc
    14041 taatcgtccg cctagacctg tttcaataat tgcaatatct actttttgtt tagcaaaata
    14101 ataaaacgcc atcgccacag taatttcaaa aaaagatggt tggatttctg caatnnnnnn
    14161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngtttgt gcatagtcca taaaacaaat
    14281 ataaagcaac ttattgtttt ttaatcttat ttttgtacaa atgaaagttt ttagaggttt
    14341 agaccaattg ccaaaattca aaaatgctgt tctgactatc gggacttatg atggcgtaca
    14401 ttatggtcat caatatatta tcaatagaat aaataaaatt gctcaagaaa ttggaggaga
    14461 aagtgttttg ctcacattct atccacatcc tcgattaatt ttgcatccta atgacaatac
    14521 tttaaaatta attacaacga ttgatgaaaa aatagaaatt ttggaaagtc ttggattgga
    14581 taatttggtt ttgattccat tctcacctga atttgctcaa caaagtgcag aagaatatat
    14641 tgaaaaggta ttaatcagaa atttcaatcc aaaatacatt tgtgttggtt acgaccataa
    14701 atttggcaaa aatagaattg gagattatca attattggaa caattaaaag aaaaatacgg
    14761 ctatcaatta gaagaaattc ctgcacaaac aattgatgaa atttctgtaa gttctacaaa
    14821 agttagaaag gctttattag aaggcgatat tgctacagca aatcattttt tggcttatcc
    14881 atttagaata aaaagcaaag taattcatgg cgataaaata ggcaaagttt taggtttccc
    14941 tactgccaat atcgaagttg ctgatgaaca caaattaatt ccagcttcag gagtatatgc
    15001 tgtttgggta aaagttgatg aacatcaatt taaaggtgca ttgagtattg gttatcgtga
    15061 aacagttttt gataatagtg aattaactat tgaagttttt attttagatt ttaataaaaa
    15121 tatttacgac aaatctattg aattaatttt tgttgattat atacgtcctc aaataaaata
    15181 cgaaaattgg gatttattga aagaacaaat agctaaagat gtagaacaaa ttaatgcttt
    15241 aatggattag ttttctgcca ttatatcata taacactttc aataacatca attactgatt
    15301 catacatatt accatataat gatggaataa tctctgtttt agtccatacg gcttgttcta
    15361 tatcttcttc ggtttgtgga attaaatttt gggattcatt aattttcata atgtaccaat
    15421 aggatagttt tatacattgc ttacctttca attcataata atgatatgtg atttgtgttt
    15481 gattgtattt taattcaatt ggctttaaaa tttctatatt tttaatacct gtctcctctt
    15541 ctacctctcg aagtgcagct tgtgagattg tttctccatc atcaatttta ccttttggca
    15601 aatcccaaga acctcttcta tgaattaata aaatttcatt tgctggatta aaaacaacac
    15661 ctccagcagc ttcaaccact tcaaagcaag caaagaaatc atttttcaat atttcaatat
    15721 ttttacaaaa gataataaca tcacttttgc tcttatcatt caaaataaat ccatttacaa
    15781 tttgttgaat atcattattt tcatcataat taagtaagat aatattggat tgtgttaaaa
    15841 tttcctggat atttgcagga ttattggtaa gaaacacttt cttatttcta ataaaaattt
    15901 tatacatttg aaccgtcatg gaaaatttag aatttaaaca aaagttagcc gaatatttgt
    15961 tacaaataaa agcaattcat tttaatcttc aaaatccata tacttgggca agtggttgga
    16021 agtcgccaat gtattgtgat aatcgattaa tattatctta tcctgatgtt agaaattttg
    16081 ttagagatgg tttgatacaa atgatagaag cctattttcc tgaagctgat tgtattgcag
    16141 gtgttgctac tgctggtatt ccacacgcta caatgatagc tgaccacctt aatttacctt
    16201 tgatttatgt acgttctaaa ccaaaagaac atggacgtga aaacttaatt gaaggagaat
    16261 taaaacatgg tatgcgtgtt gttgttattg aagatacgat atctactgga ggcagtagct
    16321 tacaggcggt agaagcatta agaaaacaag gtgctgaagt gattggtatg tgtgcaatct
    16381 ataaatatgg ttttgatgtt gctagaaaaa gatttgaaga aagtggtgtt actttaaaaa
    16441 ctttaaccaa ttacgattat gtattaagag ctggcataga aagtaaatat attgatgatg
    16501 tcgctttagt tgaattaaaa aaatgggtat cgcaaccaga tgcttggaat aaataaattt
    16561 tatgctatta ttttctatta gctttacaag ctggcaaact tatgcagttc caataattgg
    16621 agcatttatt ggatggatta caaattatat agcaataaaa atgttatttc atccacgaaa
    16681 accaatcaaa atattatttg ttacttttca tggtattttt ccaaaaaaca aagcaaggat
    16741 tgctaataaa ttaggtactg tagttcaaag agatttaatc aatttttctg atataaaaga
    16801 ccgatttaaa gatcctgatg ctttaagtaa tttcaaagaa gaaatagcaa tccgaattga
    16861 tgatgcgatt cgtgatagat tagctaaaac gccagtttta aatactattg taccagaacc
    16921 ttttattcaa ggtatccaca aaacgatagt caaagaaata gaaaccaatt tgccgcacgt
    16981 gattgattct tcgattacaa aaattgagca aaaattagat attcaagaat tagttaggaa
    17041 taaagtcgct aatttttctg atgagaaatt agagaattta cttttggata ttacagccag
    17101 agaattcaaa tttattgaat taatcggtgc tgttttaggc ttcctaatcg gaattataca
    17161 attgcttatt caagcaattt aaagcagaaa ttttattctc tgatttttta tggtgctgga
    17221 ttgggcaatt ccaaatgtat tgcttcaatc tctttcaaaa catcttccga taagtgaata
    17281 tttatcgaat caatattttc tttgagttgt tgtaaatttg ttgctccaat aatatttgca
    17341 gttaaaaaat ctctagaatt tacaaa
//
LOCUS       DAOQCW010000045        17154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1186_length_17154_cov_5.629394, whole genome shotgun sequence.
ACCESSION   DAOQCW010000045 DAOQCW010000000
VERSION     DAOQCW010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 17154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17154
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1186_length_17154_cov_5.629394"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..484
                     /locus_tag="PLS16_05540"
     CDS             272..484
                     /locus_tag="PLS16_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60577.1"
                     /translation="MKVLVEISDNDDLSFAIKVLKSLSFVKKAKPMTKDNIELWNNLI
                     EATEDVQLHKQGKVKLKSAEELLNEL"
     gene            823..1644
                     /locus_tag="PLS16_05545"
     CDS             823..1644
                     /locus_tag="PLS16_05545"
                     /inference="COORDINATES: protein motif:HMM:NF013068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNJ60578.1"
                     /translation="MSWFKKWILERQTYQTLCRDSGYSKDTLQRTFYTILKQSPSLKI
                     IKREKVNLRMDATYFSKFCLVAYQDDFDGYTQLIRFTDGEHYSEIKEDLDNLIRLGVQ
                     LESITTDGHKIVLKAIKKSVPDVLVQRCLVHIQRMCLIWLTRYPKHSSGQELRKLVLM
                     LLKIKTENDRLFWVRELHLWHKRYQDYLSEKTFNEETGRYWYKHKLVRRSYFTIKRAL
                     PNMFHYLKISTIPNTTNGIEGYFSHLKNHLDLHRGLTTKNRINFINWYMFFSNEK"
     gene            1847..2083
                     /locus_tag="PLS16_05550"
     CDS             1847..2083
                     /locus_tag="PLS16_05550"
                     /inference="COORDINATES: protein motif:HMM:NF018050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNJ60579.1"
                     /translation="MKEIECKPDLGTYLGNNCYKIRMSIESKGKGKSGGARVITYLFL
                     ENKNIYLLTIYDKSEKSDLQIGELKEMINSLSFE"
     gene            2456..3574
                     /locus_tag="PLS16_05555"
     CDS             2456..3574
                     /locus_tag="PLS16_05555"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ60580.1"
                     /translation="MIQRILHDQLQNHLFSKKAIVLMGARQVGKTTLLKNIFTNSEDV
                     LWLNGDELDVLELFKNANAVRLKLFFGNKKIVIIDEAQRIPDIGLRLKLIADNLPDIQ
                     LLATGSSSFELANHLNEPLTGRKWEYKMFPVSFKEMVNYHGLLEEKRSLNHRLVYGSY
                     PEVITNIGNEKSILKHLTDSFLYKDILMLEQIKKPDKIIKLLQALAFQIGSQVSYSEL
                     GQIVGLDSKTIEKYILVLEQSYVIFRLGSFSRNLRNELKSSKKIYFFDNGVRNALIAN
                     FEIAEMRTDIGMLWENYLVAERIKKLTYDNIWSNYWFWRTKEQKEIDFIEESNGVIMA
                     YEFKWNPNVKVKVPRNFIENYSAAFKVITPSNYEEFLL"
     assembly_gap    4018..4117
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4426..5604)
                     /locus_tag="PLS16_05560"
     CDS             complement(4426..5604)
                     /locus_tag="PLS16_05560"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNJ60581.1"
                     /translation="MEAYIVAGYRSAVGKAKKGGFRFYRPDDLAADVVKHLVASVPQL
                     DPSKIDDLICGNAIPEAEQGMQIGRMVVLRAGLPLSIGGVTVNRYCASGLETIAMATA
                     KIKAGMAECIIAGGVESMSLLPMTGWRTVLNYEIAKNTWDYYSSMGLTAEAVSKQFNV
                     TREQQDTFAFHSHQKAIKAIEEGKFKDEIVPINIEEIYLDANNKRKTRNYIVDTDEGP
                     RKDTTIEGLGKLKAVFDAKGSVTAGNSSQTSDGAAFVIVMSEKMVKELNIEPIARIIT
                     SAVVGCEPRIMGIGPVVAIPQALKQAGLKQSDIEMIELNEAFAAQSLAVIKELGLNPD
                     IINPNGGAIALGHPLGCSGAKLSIQLINEMRRQNKKYGMVSACIGGGQGIAGIIERLK
                     "
     gene            complement(5636..5992)
                     /locus_tag="PLS16_05565"
     CDS             complement(5636..5992)
                     /locus_tag="PLS16_05565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNJ60582.1"
                     /translation="MNNYKELIVWQKARVLVKSVYDLSVQLPSDEKFGLISQIKRSAV
                     SIPSNIAEGASRVSSKEFAHFLEIAYGSCFELETQLLLCLDINLLQANTLDTIITDLQ
                     EIQKMIYKLIKIKKES"
     gene            complement(6067..8454)
                     /locus_tag="PLS16_05570"
     CDS             complement(6067..8454)
                     /locus_tag="PLS16_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HNJ60583.1"
                     /translation="METKTNRAINKVAVLGSGVMGSGIALHLAGIGCEVLLLDIVPFD
                     LKEEEKANPKMRNKIVNDAFAFALKSKPAQAYSADVPKRVTLGNFDDDMQKIKDCDWI
                     IEVVIERLDIKKQVFEKVDNFRKKGSLVTSNTSGIPIHLMTEGRSEDFQKHFCGTHFF
                     NPPRYLRLLEVIPTPLTDQSVVDFFMDYGDKFLGKETVLCKDTPAFIANRVGVYTMAK
                     IYQLTAELGLKIEETDKLTGGAIGRPNTGTFKLGDLVGNDTGTKVMIGIKENCPNDEQ
                     AGAFETPAYVQFLLDNKFFGNKTKKGYYYKDEKRNSFALRLDTLQYEPVIKPKFASLD
                     VASKAERLADKVNAFIKGEDKGAELVKRSLAGLFAYVSNRIPEISDTLYAVDDALRAG
                     FAWQIGPFEYWDIVGIEKGIELAKADGLTIGTWVNDMLAAGNTSFYKTENGVRKYYDQ
                     QSKTYKAIPGAEAFIILDNLRANKPVWKNSGGTLHDIGDGVLNLEFTSKMNSLGGDVL
                     QAINKSIEIAENGYKGLVIGNDAPNFSVGANLMMIAMMAMEQEYDELDMAIRQFQRTT
                     MRCRYSGVPVVSAPHGMTLGGGCEVTLHSDAVVASAETYMGLVEVGVGLIPGGGGTKE
                     MALRTSDSLKNGDPILPTLQDNFLTVAMAKTSTSGFETYGLNLMRKGTDRVVINSKRV
                     IAEAKAEVINLFDKGYMQPAHRNDIQVLGRTGLGTLTVGVESFVAGGYISEHDAKIAK
                     KIAYVMCGGDLSAPTQVTEQYLLDLEREAFLSLCGEKKTLERIQAMLQTGKPLRN"
     gene            complement(8487..9065)
                     /locus_tag="PLS16_05575"
     CDS             complement(8487..9065)
                     /locus_tag="PLS16_05575"
                     /inference="COORDINATES: protein motif:HMM:NF028235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNJ60584.1"
                     /translation="MPKQKVTSEEILKQSFKLFREKSYHNTSMADIADACGILKGSLY
                     HYYSSKEALMSAVIDYAHTYFKEKIFSLAYNEELSPQERIERMFQKSEKTLLSDGNIM
                     GNIGVETARIVPEFANLIKDFYMEWINAVAYIFRKITNEKDAQDLAEQTVAEFEGAVM
                     MSRIFKDTRFMNNAYLRLKSRFAAITLSDFKH"
     gene            complement(9280..9846)
                     /locus_tag="PLS16_05580"
     CDS             complement(9280..9846)
                     /locus_tag="PLS16_05580"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNJ60585.1"
                     /translation="MEIEKHENQLLEQALNNDQRAFTLLMEKYQNAIQHLAFRIVGNR
                     EDAEDIMIETFSKAFDNLASYSKQYAFSTWIFKIASNTAIDFLRKKHIIKVYIDEDKS
                     IQNHTRLQFNETPEMEFMLHQRKDKIRSFLQQLAKNQKEILELRYFKELTYEEIAEQL
                     QISISNVKIQLHRAKKALASSLPKDGDW"
     gene            complement(9837..10907)
                     /locus_tag="PLS16_05585"
     CDS             complement(9837..10907)
                     /locus_tag="PLS16_05585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ60586.1"
                     /translation="MIFSFIIILLLFQITYYLFFLYYFNKKSQLDSTTYTPSISVIIC
                     AKNEAENLKNNIPKIAQQTYSDFEIIVIDDNSTDKTSIVLEQLKSSISNLKSIKISEH
                     EKIGKGKKFIISEAVKIANNEVLVFTDADCFPASNNWLKIMAQHLFNYEIVLGIAPLI
                     PKGNNLVAWLQTYETAQTMLQYISLSNSGNTYMAVGRNMMLRKTTYLQNLWSETELEI
                     ASGDDDLLIQKIATSNNTNTCTNANTFMLSSAKENYSQWIQQKIRHFSAGFLYLPKYK
                     FLLSIFLSTKILFYFLALINQNFILLLAYIFFQQCLYFFIQYKYKIRTEWQKIALLDF
                     LLVANSIILGLSNQFNIKNRWK"
     gene            10986..11504
                     /locus_tag="PLS16_05590"
     CDS             10986..11504
                     /locus_tag="PLS16_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNJ60587.1"
                     /translation="MSKYQHELETKVLKGNNVFIADNAKVLGNIYLGDDVSVWFGAIL
                     RADFDKIRIGARTNIQDGVIMHVDFGKPIEIGTDNVIGHGAMIHGAKIGSYNLIGMRA
                     TILDGAKIGNYCIIGANALVTGNMVIPDGSMVLGVPAKIVKALPMEAVKSGVALGVEE
                     YILEKNKYLGIK"
     gene            complement(11590..11757)
                     /locus_tag="PLS16_05595"
     CDS             complement(11590..11757)
                     /locus_tag="PLS16_05595"
                     /inference="COORDINATES: protein motif:HMM:NF018961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HNJ60588.1"
                     /translation="MAIVKVIEVIASSEKSFDDAVKNALSEASKTVKNIDSIWVKDFK
                     CHVSNQQIATY"
     gene            12079..12294
                     /locus_tag="PLS16_05600"
     CDS             12079..12294
                     /locus_tag="PLS16_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60589.1"
                     /translation="MENTMLIEVTNQKALKLLHELEELHLIKVLKENFPSTKIKLSDK
                     YRGILSKEKGQSLNEHIKEMRNEWNNI"
     gene            12279..12662
                     /locus_tag="PLS16_05605"
     CDS             12279..12662
                     /locus_tag="PLS16_05605"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNJ60590.1"
                     /translation="MEQYLIDTNVVSDYLSASLSKVGMDFLDTVIDTIPNLSVISKIE
                     LLCWKTDKITTEGVKNFVADSIILDIAPNVISYCVAVRKSKKIKTPDAIIAATALAHG
                     YILITNNEKDFLNIKGLKIINPHKM"
     gene            complement(12786..14072)
                     /gene="murF"
                     /locus_tag="PLS16_05610"
     CDS             complement(12786..14072)
                     /gene="murF"
                     /locus_tag="PLS16_05610"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNJ60591.1"
                     /translation="MNTIQEIYPLFLKSAGISTDTRNIQPNQIYAALKGANFDGNTMA
                     EDALNKGAIYAIIDNPAYKKNDNYFLVDDVLSFLQQLGLYHRKQIPLKALIAVTGSNG
                     KTTTKELINAVLATTYKTHYTKGNLNNHFGIPRTLLEMPLDTEIAVIEMGANHQKEIA
                     AYCTYVEPNYGVITNIGSAHLEGFGGIEGVKKGKGELFDYLKLHNGIIFYNLDSEHIQ
                     SIINEKNIEHTITYGKTKADYQAKIITETPLLSISFEDITIQSHLFGGYNFDNILCAI
                     AIGKHFNVSNDLIKSAIEEYIPNNKRSELITIGTNSIICDYYNANPTSTKHALESFAK
                     SDATNKMVILGDMFELGEYALKEHQQIVDLAEKLNFDTIVLVGSIYAQTQTSKDVIKV
                     QDAQAAKVWLNEYNPQHTQILLKGSNGMKMEKVIEL"
     gene            complement(14142..15632)
                     /locus_tag="PLS16_05615"
     CDS             complement(14142..15632)
                     /locus_tag="PLS16_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNJ60592.1"
                     /translation="MKRLLFISIVALTFASACKGGKGNDSSSTTGWKYNDKDWGGFQV
                     AQNVQQITGPNLVLVEGGTFVMGATEDNLLYEFDNIARRVSVSSFYMDETEVKNVDYR
                     EYIYWLQRVFGGDHPEFVSAAKPDSLCWRRNLAYNEPYVKYYFTHPSYNTYPVVGVSW
                     LQASEYCKWRTDRVNEMLLIKKGYLSTNPDQINEDNFNTEAYMTGLYQGKAGTNPGIE
                     NLGPEGGTRNLNMSDGVLLPEYRLPTEAEWEYAALGLRGNMPLQGEEVISDRRIYPWD
                     GATFRYQKHGKQQGWFMANFMRGRGDYMGVAGALNDNAEITSDVYANFPNDFGLFNMS
                     GNVNEWVQDVYRPLTEAIAEDLNTFRGNDFMKVSPEEGLDEKGRIKYVRQDDEELANR
                     FNYRKAFALDYNDGDSSSFVSYNYGISTLINNKSRVYKGGSWRDRGYYLSPGTRRFIN
                     EDQSSDDLGFRCAMVRVGSPDGNIFGGKGFTEMKKTITKNQKARKF"
     gene            15837..>17154
                     /locus_tag="PLS16_05620"
     CDS             15837..>17154
                     /locus_tag="PLS16_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60593.1"
                     /translation="MFLQNSNVRFLTIFYLFVFLFIPICIASAKETIFIQAIKYGKNF
                     TFSSAYKDESNQQLPYYVYQKQFNGNEFDVQFSNLNYINVDVEFSTSWNISPDLLISK
                     QIVKEKGRTQANVIINPYRLNGQQLQLLQSFDIDIIAIENNSNLRPSSTLQSYVNNSV
                     LANGDWYKFGVTKAGIYKLDFNFFKQLGINTGNIHPNNIRIFGQFGGMLPELAGTNRT
                     DDLKEYPIKVITQNPNQFQQGDFVLVYCKGPDLWNYDGSKQQFKIIKNLYSTTQNLFI
                     TTNNGSGLRIGSISGNTLAENFATNTYDAIDFVEQEIENLGMSGRTFVGDRFINQTNK
                     TYQFNFPNIISNQPLKIWTNVVAVANTFSTMNISMGNNVRNISMSPLQQEYPAVYSYG
                     MPAEQSFLFNNPSSNVQLNYQYQSNDFSAKAYLDFINVQAKCNLVYD"
BASE COUNT         5860 a         2628 c         2556 g         6010 t
ORIGIN      
        1 cactaaccac tgtgaaaact ctgtgtaaaa aacttagtgt cctttgtggt taaaagagca
       61 ttaagtagtg agaaattcat tacttcattg ctacattact acattttttc attactacat
      121 tatttcatta atctttgtga aaactctgta aaaaaaaact agtgttcttt gtggttaaaa
      181 aaatgagtat taagtaacta aaagctaaaa aagtctaata aaacactatt tctatgattt
      241 ttgtatatat ttgtattata aggttttaaa tatgaaagta ttagttgaaa tttctgataa
      301 tgatgatttg tcttttgcta ttaaagtctt aaaaagtcta tcctttgtga aaaaggcaaa
      361 accaatgaca aaggataata ttgaactatg gaataatctt attgaagcaa cagaagatgt
      421 tcagttacat aagcaaggaa aagtaaaact aaaatctgct gaagagttgt taaatgaact
      481 ataatataat tactacgcgg cgatttgaaa aggaaataaa gcgattagct aaaaaattca
      541 catcgtttaa aagtgatttt tcaaaattaa ggcataatac tgaaaaatag ttggcagaaa
      601 gctctgtatt gactatgaat aaagggttta acaaaagttt tatgaaacgt gtttcataaa
      661 actttttctt tgtctaaaaa atggtaaaaa aaacgatgtt ggtcttgtaa aagtttggaa
      721 gttattggtt ggggaaaaca aaataataaa caaaggttta aatgcaaaaa ttgcggtttg
      781 ttgttcacaa ataatcgccc agaacaaaaa gttaaaaacc gtatgtcttg gtttaaaaaa
      841 tggattcttg aaaggcaaac ctaccaaact ctttgtcgag atagtggcta ctcaaaagat
      901 acacttcaaa ggacttttta tactattttg aaacaatctc caagcctaaa aatcatcaaa
      961 cgagaaaaag taaatctgag aatggatgca acttattttt ccaagttctg tttggtggct
     1021 taccaagatg attttgatgg ctacacacag cttatccgtt ttacagacgg cgaacattat
     1081 tcagaaataa aggaggattt agataatttg atacgcttag gtgttcaact tgaaagcatt
     1141 acaactgatg gacataaaat tgttctgaaa gctatcaaaa aatctgttcc tgatgtactt
     1201 gtacaacgat gtctagtgca tatacaacga atgtgtttga tatggcttac tagataccct
     1261 aagcatagtt ctggtcaaga acttagaaaa ttggttttga tgttacttaa aataaaaaca
     1321 gaaaatgata gattattttg ggtaagagaa cttcatcttt ggcataaaag atatcaggat
     1381 tatctttcag aaaagacctt taatgaagaa acaggaagat attggtacaa gcataaactt
     1441 gtccgtcgtt cttattttac cataaaaaga gccttgccaa atatgtttca ttatttgaaa
     1501 atatcaacta tcccgaatac aacaaatggt atcgaaggtt attttagtca tctaaaaaat
     1561 catttagatt tgcatagagg attaacgact aaaaatagga taaatttcat caattggtat
     1621 atgtttttct ctaacgagaa ataggttttt ttagccatat tgcttaccaa agaatgttat
     1681 aaaaaaggac gataatttat cgtcctcatt cttagtttaa gcatcaatct tattgctgct
     1741 tggttgctcc tcagcagagc caatttcctc ttcagattga cagtcgaagt ataatacttt
     1801 tttttataaa ttccaccaac tatttttcag tacattacca aaattaataa aagaaattga
     1861 atgtaaacca gatttaggta cttatcttgg caataattgt tataagatac gtatgtcaat
     1921 tgaaagcaaa ggtaaaggaa agtcaggagg agcaagagta attacttatt tattccttga
     1981 aaacaagaac atctacttac ttacaattta tgataaaagc gaaaaaagtg atttgcaaat
     2041 tggagaactt aaagagatga taaatagcct ttcatttgaa taagacgaaa acgaatggag
     2101 aattaaaaat tgaaaattga aaattgaaaa ttgagaatta aaaattgaaa attgaaaatt
     2161 acgaattacg aattgagaat taacaactaa ccactaacca ctaaccacta accactgtga
     2221 aaactctgtg taaaaaactt agtgtccttt gtggttaaaa aaatgagtat taagtattaa
     2281 gtattgagta ttaagtagta agatgagtag aaagtattga gtattaagta ttgagaaatt
     2341 cattactaca ttatttcatt atttcattat tttatggttc aaatccaaaa aagttgataa
     2401 attccaagaa tcaaatccaa aaaagttgtt atatttgttt aagtcaaaac aatgtatgat
     2461 acaacggata ttacatgatc aacttcaaaa ccacctcttc tctaaaaagg caatagtctt
     2521 aatgggagca agacaagtag gaaaaaccac acttttgaaa aatattttta caaattcaga
     2581 agacgtttta tggctgaatg gagacgaact tgatgtatta gaacttttta aaaatgcaaa
     2641 tgcagtgagg ttgaaacttt tttttggaaa taaaaagata gtaataattg atgaagcaca
     2701 gagaatacca gacattgggt taaggttaaa attgattgct gacaacttac ctgatattca
     2761 actattagca acaggaagtt cttcatttga acttgcaaac catctgaatg aaccactaac
     2821 aggacgaaaa tgggagtata aaatgtttcc tgtttctttt aaggaaatgg tcaattacca
     2881 tggactttta gaggaaaaac gttcactaaa tcatcgctta gtttatggct cttatccaga
     2941 agttataaca aatataggca atgaaaagtc gattttgaaa cacctaacag atagtttttt
     3001 atacaaggat attttgatgt tggagcaaat taagaaacct gataagataa taaaattact
     3061 tcaagcctta gcctttcaga taggctctca ggtatcctat tccgagcttg gacaaattgt
     3121 tggcttagat tctaaaacaa tagaaaaata tattctagtt ttagagcaat catacgtaat
     3181 atttagatta ggttcattca gtagaaattt acgtaatgaa ttaaaaagta gtaagaaaat
     3241 atatttcttt gacaacggtg ttcgcaatgc actaattgca aattttgaaa tagcagaaat
     3301 gagaactgat attggaatgc tttgggagaa ttacctagtt gctgagcgaa tcaaaaaatt
     3361 gacctatgat aatatttgga gtaattattg gttttggcgg acaaaagagc aaaaggaaat
     3421 tgatttcata gaagaaagta atggcgtaat tatggcttat gaattcaaat ggaatcccaa
     3481 tgtaaaagta aaagttccaa ggaattttat cgagaattat tcagcagcat ttaaagtcat
     3541 aacaccaagt aattatgaag aatttttact ataaaaccaa gacttttttg agtaataagt
     3601 agaaagtatt aagtattaag agtcaagatt tttagagcca agaaccaaga ctttttttga
     3661 gtagaaagta ataagtatta agtattgaga agttaattat tacattacta cattacttca
     3721 ttaataaaat cctgctaatc atttaatcct aaaaatcata gttcagacaa ttgccgagaa
     3781 tcaagattat tagaaccaag aatcaagacc ttttttgagt agaaagtaat aagtattaag
     3841 tagtaagtat taagtagtga gaaattcatt actacattat tacattactt cattactaca
     3901 ttaaaaaaat cctgattcag acaattgccg agtattgaga agttaattat ttcattattt
     3961 cattaatctt tgtgaactct gtgtaaaaaa cttagtgtcc tttgtggtta aaaaagtnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatt aagtagaaag tattaagtag
     4141 aaagtattaa gtagaaagta ttaagtagaa agtattaagt agaaagatga gtagaaagta
     4201 ataagtatta agtattgaga agttaattat tacattacta cattattaca ttactacatt
     4261 aaaaaaaatc aaggttcaga caattgctga gaatcaaagc tttatggtgt ccgtacaaaa
     4321 aataggctgc cctttacaga cagcccattt ttttgtttat caagtatcaa gactcaggta
     4381 gtaagaaaac taatgcctat tccctaatgc ctattcccta cgtaattatt tcaatctttc
     4441 aataatacct gcaatacctt gtccgcctcc aatacaagca cttaccatac cgtatttttt
     4501 attctgacga cgcatttcat tgattaattg aatagataat ttagctccag aacaacctag
     4561 tggatgaccc aatgcaattg ctccaccatt tggattgata atatcaggat ttaaacctaa
     4621 ttctttgata acagctaatg attgagcagc aaatgcttcg tttaattcaa tcatttcgat
     4681 atcactttgt tttaatccag cttgttttaa cgcttgtggt atagcaacaa caggtccgat
     4741 acccataatt cttggctcac aaccaactac agcactagta ataattcttg cgattggctc
     4801 aatatttaat tcttttacca ttttctcgct cattacaatt acaaaagcag ctccatcaga
     4861 agtttgagat gaattacctg cagttacgga acctttagca tcaaaaaccg cttttagttt
     4921 tcctaaacct tctattgtag tatcttttct tggaccttca tcagtatcta ctatataatt
     4981 tctagttttt cttttattat tagcatctaa gtagatttct tcaatattaa taggaacaat
     5041 ttcatcttta aattttcctt cttcaattgc tttaatcgct ttttgatgag aatgaaaagc
     5101 aaaggtatct tgttgttctc tagttacatt gaattgttta gaaactgctt ctgctgttaa
     5161 gcccattgag gaataataat cccaagtgtt tttagcaatt tcataattta aaacggttct
     5221 ccagcctgtc attggcagta atgacataga ttctacgcca ccagcaatga tacattctgc
     5281 cattcctgct ttgatttttg cagttgccat agcaatagtt tctaaaccag atgcacagta
     5341 tctatttacg gtaacaccgc caatacttaa tggaagtcct gctctcaaga caaccattct
     5401 tccaatttgc attccttgtt ctgcttcagg aattgcattt ccacaaatta aatcatcaat
     5461 ttttgatgga tctaattgag gaactgatgc aactaaatgc tttacaacat ctgctgctaa
     5521 atcatcggga cgataaaatc gaaaccctcc tttttttgct ttgcccaccg cactgcggta
     5581 tccggctacg atatatgctt ccatattttt agtatttagt aatgagtatt aagtattaag
     5641 actctttttt aattttaatt aatttataaa tcattttttg gatttcctgt aaatcagtaa
     5701 taatcgtatc taaagtattt gcttgtaata agtttatatc aaggcataat aataattgag
     5761 tttctaattc aaaacaagaa ccataagcaa tttccagaaa gtgagcaaat tcttttgagg
     5821 aaactctact tgcaccttct gcaatattag aaggaataga aacggcactt cttttgattt
     5881 gactaattaa accaaatttc tcatcagaag gaagttgtac acttaaatca tatactgatt
     5941 taaccaatac tcttgccttt tgccatacta ttaattcttt atagttgttc attcttaact
     6001 tttgctaatt atattttatg gcgtattact catgtctcaa atctcactac ttaaaatttc
     6061 atactactaa tttctcaaag gttttcctgt ttgcaacatg gcttggattc tttctaaagt
     6121 tttcttttcg ccacaaagtg ataagaatgc ttctctttct aaatccaata gatattgttc
     6181 cgttacttga gtaggagctg ataaatctcc accacacatt acataagcaa ttttcttagc
     6241 aatttttgca tcgtgctcgg aaatatatcc acctgcaaca aatgattcaa cacctactgt
     6301 aagtgtacct aaaccagttc ttcctaatac ttgaatgtca tttctgtgag ctggttgcat
     6361 atagcctttg tcaaataaat tgatgacttc tgctttcgct tctgcaatta ctcttttaga
     6421 attgataaca actctgtcag ttcctttacg cattaagttt aagccataag tttcaaaacc
     6481 agaagttgaa gtttttgcca tagcaacagt taagaaatta tcttgaagtg tcggtaaaat
     6541 tgggtcgcca ttttttaaag aatctgatgt acgtaacgcc atttctttag ttccgccacc
     6601 acctggaatt aaaccaacgc caacttctac taatcccata taagtttcag cagatgcaac
     6661 aacagcatca gagtgtaaag taacttcaca accaccacct aatgtcatac cgtgaggagc
     6721 agaaaccact ggaacaccag aatatctaca acgcatagta gttctttgga attgacgaat
     6781 agccatatcc aattcatcat attcttgttc cattgccatc atcgcaatca tcattaagtt
     6841 ggcaccaact gagaagtttg gagcatcatt tccgattact aatcctttgt atccgttttc
     6901 agcaatttca atagatttat tgatagcttg taatacatcg cctcctaatg aattcatctt
     6961 agaagtaaat tctaaattca aaacaccatc tccgatatca tgtaaagtac caccagaatt
     7021 tttccaaaca ggtttgtttg ctcttaaatt atctaagata ataaatgcct cagcacctgg
     7081 aattgctttg taagttttag attgttgatc gtagtatttt cttacaccat tttcagtttt
     7141 ataaaaagaa gtattgccag ctgccaacat atcattaacc caagttccga tagttaaacc
     7201 gtcagctttt gcaagttcaa tacctttttc aatgccaacg atatcccaat attcaaatgg
     7261 accaatttgc caagcaaagc cagcacgtaa agcatcatca acagcatata aagtgtcaga
     7321 aatttcagga atacggtttg aaacataagc aaataatcca gctaaagaac gttttaccaa
     7381 ttcagcacct ttatcctctc ctttgatgaa tgcatttact ttatctgcta atctttcagc
     7441 tttagaagcg acatctaaag aagcaaattt aggtttgata actggttcat attgcaaagt
     7501 atctaaacgt aatgcaaatg aatttctttt ctcatcttta taataataac cttttttagt
     7561 tttattaccg aagaatttat tgtctaataa aaattgaaca taagcaggtg tttcaaatgc
     7621 accagcttgt tcgtcattag gacaattttc tttgatacct atcatcactt tcgtgccagt
     7681 atcattgcct actaaatcgc ctaatttgaa agttccagta ttaggacgac caatagcacc
     7741 gccagtcaat ttatccgttt cttcaatttt taagcctaat tcagctgtta attgatagat
     7801 ttttgccata gtataaacac ccactctgtt tgcaataaat gcaggtgtgt ctttacataa
     7861 aacagtttct tttcctaaga atttatcgcc ataatccata aagaaatcaa ccacactttg
     7921 gtcggttaat ggcgttggaa ttacttctaa taatcttaaa tatcttggtg gattaaagaa
     7981 gtgtgtacca cagaaatgct tttggaaatc ctcacttcta ccttcagtca ttaaatggat
     8041 aggaatacca gatgtattag aagttaccaa tgaacctttt tttctgaaat tatccacttt
     8101 ttcaaatact tgttttttga tgtctaatcg ctcaattaca acttcgataa tccaatcgca
     8161 gtctttaatt ttttgcatat catcatcaaa atttccaaga gtaactcttt ttggaacatc
     8221 tgcactgtat gcttgtgcag gtttagattt taatgcaaaa gcaaaagcgt catttacgat
     8281 tttgtttcgc attttggggt tagctttttc ttcttctttc aagtcaaaag gaacgatatc
     8341 taacaatagt acttcgcagc ctatacctgc taagtgcaat gcaataccag aacccataac
     8401 tccagaacca agaacggcta ctttgttaat tgctctgttt gtttttgttt ccatgtaata
     8461 aaaaaatcaa tttaaaaaat atataattaa tgtttgaaat cagataatgt aatggcagca
     8521 aatctagatt ttaatcgcag ataggcattg ttcataaatc tggtatcttt aaaaattcta
     8581 ctcatcatta ctgcaccttc aaactcagct actgtttgct ctgctaagtc ttgagcatct
     8641 ttctcattag taatttttct gaatatatag gcaacggcat taatccattc catataaaaa
     8701 tctttaatca aattggcaaa ttctggaact attctggcag tttctacacc aatatttccc
     8761 ataatatttc catcagacaa gagtgttttt tcagattttt ggaacattct ttcgatgcgt
     8821 tcttgtggcg ataattcctc attataagct aatgaaaata ttttttcttt aaaataagta
     8881 tgagcgtagt cgataaccgc actcatcaaa gcttctttgg aagaatagta atgatacaag
     8941 ctgcctttta gtatgccaca agcatcagca atatcagcca ttgatgtatt gtgataactc
     9001 ttttctcgaa acagtttaaa cgactgttta agaatttctt ccgatgtaac tttctgttta
     9061 ggcacgtatt aaaataacac ataaaaataa tcaatgcatg cattgtatca aacgttcgtt
     9121 tggcataata ataatttgat aataatattt ttaggaaaaa ttagtcaatt ttaaagtttg
     9181 taaaatatta attatactaa tgtattttta aaaacaataa aatcgataaa ctaaaagtta
     9241 taacttttgt acgtttaatc ggtataaaaa tattttaatt taccaatcac catcttttgg
     9301 taagctactc gctaaagctt ttttagctcg gtgcaattga attttaacat ttgaaattga
     9361 aatttgtaat tgttcagcaa tctcttcgta agtcaattct ttaaaatatc gtaattctag
     9421 tatttctttt tgatttttgg ctaattgttg gagaaaactt ctgattttgt cttttctttg
     9481 atgtagcata aattccattt caggtgtttc attaaattga agtcgagtat ggttttgaat
     9541 gcttttatct tcgtcaatat aaactttaat aatgtgtttt tttcttaaaa aatcgatagc
     9601 ggtattcgat gcaattttaa aaatccaagt agaaaatgca tattgtttgg aataagaagc
     9661 taaattatca aaagctttag aaaaagtttc aatcataata tcttcagcat cctctctatt
     9721 gccaacaatt ctaaaagcca aatgctggat tgcattttga tatttctcca ttaatagcgt
     9781 aaaagcacgt tggtcgttgt ttaacgcttg ttccaaaagt tggttttcat gcttttctat
     9841 ttccatctat ttttgatgtt gaattggttt gataaaccca agataatact atttgcgacc
     9901 aaaaggaaat ccaataatgc tattttttgc cattctgttc ttattttgta tttatactga
     9961 atgaagaaat ataagcactg ttgaaagaaa atataagcta ataaaagaat aaaattttgg
    10021 ttgattagag ccaggaaata gaataatatt ttagtggata agaatatgct aagcaaaaat
    10081 ttatatttag gcaagtataa aaaaccagca gaaaaatgtc ttattttttg ttgtatccat
    10141 tgcgaataat tttcttttgc agaagagagc ataaatgtat ttgcatttgt acaagtattg
    10201 gtattattag aagttgcaat cttttgtatc aataaatcat cgtcgccaga tgctatttct
    10261 aattccgttt ctgaccaaag attttgtaaa taggtagttt ttcgtagcat catatttctg
    10321 ccgactgcca tataagtatt gccagaattt gataacgaaa tatattgcaa catagtttga
    10381 gccgtttcat aggtttgtaa ccaagcaact aagttatttc ctttcggaat taatggtgca
    10441 atgcctaaaa caatttcata attaaataaa tgttgagcca ttattttcaa ccaattatta
    10501 gacgctggaa aacaatctgc atctgtaaaa actaaaactt cattattggc tattttaact
    10561 gcttcgctga tgataaattt tttccctttc ccaatttttt catgttcaga gatttttatt
    10621 gattttaagt ttgaaataga agattttaat tgttctaaaa cgattgatgt tttatctgta
    10681 gagttatcat caattacaat aatttcaaaa tctgagtacg tttgttgagc aattttaggg
    10741 atattatttt ttaaattttc agcttcattt tttgcacaaa taataacact aatagatgga
    10801 gtgtaagtag tactatctaa ttgacttttt ttattaaaat aataaagaaa aaatagataa
    10861 taagttatct gaaatagtag tagtataata ataaaactga aaatcataat tggtataaac
    10921 aaaatcgcta agatgtaaaa tacaaaatat tattaagcag agaataattt tgtatattta
    10981 tatttatgag taaatatcaa catgaattag aaactaaagt acttaaaggt aataatgttt
    11041 ttattgcaga caatgcaaag gttttgggaa atatttattt aggagatgat gtgtcggttt
    11101 ggtttggggc aattctacgt gccgattttg ataagattag gattggtgca agaacgaata
    11161 tacaagatgg tgttattatg catgtagatt ttggtaagcc tatcgaaatc ggaacagata
    11221 atgttattgg acacggagcg atgattcatg gagcaaaaat tggttcctat aatttgattg
    11281 gaatgcgagc tacaatttta gatggtgcca aaataggtaa ttactgtatt attggtgcaa
    11341 atgcattggt tacggggaat atggttattc cagatgggag tatggtttta ggtgtacctg
    11401 ctaagattgt aaaagcctta ccaatggaag ctgtaaagtc aggtgttgct ttgggtgtag
    11461 aagaatatat tttggaaaag aataagtatt tgggtataaa ataaaaagga gcaaaactta
    11521 atataatttg ctcctcttag tttttggtaa attaagttat tctactctaa atgaaatttt
    11581 gcagataact cagtaagtag caatttgctg attgctaaca tgacatttaa aatcttttac
    11641 ccaaatagaa tctatgttct taactgtttt tgatgcttct gatagtgcat tctttactgc
    11701 atcatcaaaa cttttttcgg aagaagcaat aacttcaata acttttacaa tcgccataat
    11761 ttaagtttta aaaaatgaat aatgtattaa agttatagtc aaatagatgg aactacaact
    11821 ttttaacttt taataagaat atgaatgatg gttttaagag attacctttt ccattttcat
    11881 tccattagag ccttttaaaa gaatttgagt atgttgtgga ttatactcat ttagccatgt
    11941 cctagagcaa ctcaataatt tattaatgtc gcaatattta ttgctaataa caatttttct
    12001 aatatgtttt gcctggttac ttttacaata tttttttatg tatttttgtt atgtagttac
    12061 ttattaaact tttgacttat ggaaaataca atgctcattg aagttaccaa tcaaaaagcc
    12121 ttaaaacttt tacacgaatt ggaagaatta catttaataa aggttctgaa agaaaatttt
    12181 ccttcaacaa aaataaaact gtctgataaa tacagaggta ttctcagtaa agagaaaggt
    12241 caaagtttga atgaacatat caaagaaatg cgtaacgaat ggaacaatat ttgattgaca
    12301 ccaatgtagt ttcagattat ttgtcggctt ctttatctaa agtgggtatg gatttcttag
    12361 atacagtaat tgatactatc cctaatcttt ctgtaatttc taaaatagaa ctactttgct
    12421 ggaagacgga taaaattact acagaaggtg taaagaattt tgttgcagat agtataatat
    12481 tagacattgc tcctaatgta atttcttatt gtgtagctgt acgaaaaagc aaaaaaataa
    12541 aaacacctga cgctattatt gctgcgactg ctttggcaca tggttatatc ctaattacta
    12601 acaatgaaaa agatttttta aatatcaaag gattgaaaat tattaatcca cataaaatgt
    12661 aaaaaatatt taattggcaa agtactaata tctattttct aactccttgt tcctgattgt
    12721 tttgatctaa ttatgtattc ttgggaatta gatattttgg attaaaatag cacttctgca
    12781 taaatctata attctattac cttttccatt ttcattccat tagagccttt taaaagaatt
    12841 tgagtatgtt gtggattata ctcatttagc caaacctttg cagcttgagc atcttgtact
    12901 ttaattacat cttttgatgt ttgtgtttga gcgtaaatac tcccaaccaa tacaattgta
    12961 tcaaaattta atttttctgc caaatctaca atttgctgat gttcttttaa ggcatattcg
    13021 cctaattcaa acatatcacc taggataacc attttatttg tagcatcaga ttttgcaaaa
    13081 ctttccaaag catgtttagt gctagttgga ttcgcattat agtaatcaca aatgatggag
    13141 ttggtaccta ttgtaattaa ttctgaacgt ttattgtttg gaatatattc ttcaatagct
    13201 gattttatta aatcattaga tacattaaaa tgtttcccaa ttgcaatagc acataatata
    13261 ttgtcgaaat tataacctcc aaaaagatga ctttgtatcg ttatatcttc aaatgaaatg
    13321 gataggagag gtgtttctgt tataattttt gcttgataat cggctttagt ttttccataa
    13381 gttatggtat gttcaatatt tttttcatta attattgatt gaatatgctc actatctaaa
    13441 ttataaaaga taattccgtt gtgcagtttt aggtaatcaa ataattcgcc ttttcctttt
    13501 tttacgccct caataccgcc aaatccttct aggtgagcac taccaatatt cgtaatcacg
    13561 ccataatttg gttcaacata agtacaataa gctgcaattt ctttttggtg attggctccc
    13621 atttcaataa ctgcaatttc agtatctaat ggcatttcca atagggttct tggaatacca
    13681 aaatgattgt ttaaatttcc ttttgtataa tgtgttttat aagtagtagc caacacagca
    13741 ttgattaatt ctttagtagt tgttttccca ttcgagccag taactgcaat taatgctttt
    13801 aatggaattt gttttctatg atataatcct aattgttgta agaaagataa aacatcatct
    13861 actaaaaagt aattatcatt ctttttatat gcaggattat caataattgc ataaattgct
    13921 cctttattta aagcatcttc agccattgta ttcccatcaa aatttgctcc ttttaatgca
    13981 gcataaattt gattaggttg tatatttcta gtgtcggtag agatacctgc actttttaaa
    14041 aacaatggat atatttcttg tatagtattc atatataggg atggtaaaaa taaaaaaagt
    14101 cccgatatat tatcgggact ttttttattt atttgagtaa tttaaaactt tctcgctttt
    14161 tgatttttag taatggtttt tttcatttct gtaaaacctt tacctccaaa gatgttacca
    14221 tcaggagaac ctactcttac cattgcacat ctaaagccta aatcatcaga agattggtct
    14281 tcgttgataa atcttcttgt acctggagat aaataataac ctctatctct ccaagaacca
    14341 cctttataaa ctctagattt attattaatt aatgttgaaa taccatagtt ataagaaaca
    14401 aaagaagaag agtcgccatc attataatct aatgcaaatg ctttgcgata gttgaatctg
    14461 ttagctaatt cttcatcatc ttgtcttaca tatttaattc tacctttttc atctaaacct
    14521 tcttcaggag aaactttcat aaaatcgtta cctctaaaag tatttaaatc ttcagcgata
    14581 gcttcagtta aaggtctata aacgtcttga acccactcat taacattacc agacatatta
    14641 aataatccaa aatcattagg gaagtttgca tatacatcag aggtaatttc agcattatca
    14701 tttaaagcac cagcaacacc catgtagtcg cctcgtcctc tcatgaagtt agccataaac
    14761 caaccttgtt gtttgccatg tttttggtat ctgaaagtag caccatccca aggatagatt
    14821 cttctgtcag atattacttc ttctccttgt aatggcatat tgcctcttaa acctaaagca
    14881 gcatattccc attcagcttc tgttggaagt ctatattcag gtaataatac accatcactc
    14941 atatttaagt ttcttgtacc accttcaggt cctaagttct caataccagg atttgttcct
    15001 gcttttcctt gatatagacc agtcatataa gcttcggtat tgaagttatc ttcatttatt
    15061 tgatctggat tggtagataa atatcctttc ttaattaata acatttcatt taccctgtca
    15121 gttctccatt tgcaatattc acttgcttgt aaccaagaaa caccaactac tggatatgta
    15181 ttgtaggatg ggtgtgtgaa atagtatttt acataaggtt cattgtatgc taagtttctt
    15241 ctccaacaaa gtgaatctgg ttttgcagca gatacgaact ctgggtgatc gccaccaaat
    15301 actctctgta accaatagat atattctctg taatctacgt ttttaacttc agtttcatcc
    15361 atatagaatg aagaaactga aactctacgt gctatgttgt caaattcgta aagtaagttg
    15421 tcttcagttg cacccattac aaatgtacca ccttctacta aaaccaagtt tggtccagta
    15481 atttgttgta cattttgagc tacttgaaag ccgccccaat ctttgtcgtt gtatttccaa
    15541 cctgttgtcg aacttgagtc atttccttta ccgcctttac aagcagaagc aaaagtcaag
    15601 gctacaatag aaataaaaag aagtcttttc ataactgtta tttattgaat ttaattttcc
    15661 taaagataca aaagtaatat aatgacacta aatatccaac acttttttat tttattatta
    15721 aacgaatttt ataaaatatt agtgtgtata ttcgttaaaa gttttttatt tttattaaga
    15781 atatttaatt taaaggcttt ttcatagatt ttgactaaat ttaattgata atttatatgt
    15841 ttttacaaaa ttctaatgtt cgatttttaa caatatttta tctctttgtt tttttattta
    15901 tccctatctg tattgcttct gcaaaagaaa caatttttat acaagcgata aaatatggta
    15961 agaattttac tttttcgtct gcttataaag atgagtcaaa ccaacaattg ccttattatg
    16021 tttatcaaaa acaatttaat ggaaatgaat ttgatgtcca attttctaat ctaaattata
    16081 ttaatgttga tgttgaattt tctacgtctt ggaatatttc accagatttg ttgatttcta
    16141 aacaaattgt aaaagagaaa ggcagaaccc aagcaaatgt aataatcaac ccatatagat
    16201 taaatggtca gcaattacaa ttacttcaat ctttcgatat tgatattatt gcgatagaaa
    16261 ataattcaaa tttacgacca agtagcactt tacaatctta tgtaaataat tcagtattag
    16321 caaatggaga ttggtataaa tttggtgtta caaaagcagg tatatataag ttagatttta
    16381 atttttttaa acaattagga attaatacgg gaaatattca tcctaataat attagaatat
    16441 ttggacaatt tggtggaatg ttaccagaac tggctggaac gaatagaaca gatgatttaa
    16501 aagagtatcc aataaaagtt atcactcaaa atcctaatca attccaacaa ggagattttg
    16561 tattagtata ttgtaaaggt cctgatttgt ggaattatga tggatctaaa cagcaattta
    16621 aaataatcaa aaacttatat tcaacaactc aaaatttatt tattacgaca aataatggaa
    16681 gtggattaag aataggaagt ataagtggaa atacattagc tgaaaatttt gccacaaata
    16741 cttatgatgc tattgatttt gtggagcaag aaattgaaaa tttgggtatg tctggtagaa
    16801 cttttgttgg tgatagattt attaatcaaa caaacaaaac ctatcaattt aattttccga
    16861 atattatttc aaaccaaccc ttaaaaattt ggacaaatgt tgttgcagtt gccaatacgt
    16921 ttagtacgat gaatatttca atgggcaaca atgttagaaa tattagtatg tcgcctttgc
    16981 aacaagaata cccagcagtt tatagttatg gaatgcctgc tgaacaatca tttttattca
    17041 ataatccgtc ttctaatgtg caattaaatt atcaatatca aagcaatgat ttttcagcaa
    17101 aagcttattt agattttata aacgttcaag caaagtgtaa tttagtttat gatg
//
LOCUS       DAOQCW010000046        17024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1202_length_17024_cov_5.326596, whole genome shotgun sequence.
ACCESSION   DAOQCW010000046 DAOQCW010000000
VERSION     DAOQCW010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 17024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17024
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1202_length_17024_cov_5.326596"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="PLS16_05625"
     CDS             <1..432
                     /locus_tag="PLS16_05625"
                     /inference="COORDINATES: protein motif:HMM:NF013104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HNJ60594.1"
                     /translation="GLVAITPAAGYVSYGPSLFIGFAASLISNYAVVAFKKTRIDDTL
                     DVFPCHGVGGMMGMILTGVFAADYGTGITDFGLAYGQTKTFFAEIIALGSVAAYVFFG
                     SYAIYYITNKIKPIRVSKTDEELGLDISQHGESYTFAKINL"
     gene            complement(500..1381)
                     /gene="thyA"
                     /locus_tag="PLS16_05630"
     CDS             complement(500..1381)
                     /gene="thyA"
                     /locus_tag="PLS16_05630"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNJ60595.1"
                     /translation="MKQYQDFLQHILDKGTIKEDRTGTGTISVFGYQMRFDLNDGFPL
                     VTTKKLHLKSIVYELLWFIAGDSNIEYLCKNGVNIWNDWPYQKYKSSSDFSGESMKEF
                     AAKIATDHEFALKYGELGPVYGKQWRHWKDEHGNEIDQITNLIHQIKTNPDSRRLIVS
                     AWNVAEIEQMTLPPCHTIFQFYVIDGKLSCQLYQRSADTFLGVPFNIASYALFTMMVA
                     QVCGLGLGDFVHSFGDAHIYSNHIDQVKLQITRTPYSLPKMKINPEVKSIFDFKYEDF
                     TLENYEAHPHIKGEVAV"
     gene            complement(1562..2896)
                     /gene="purB"
                     /locus_tag="PLS16_05635"
     CDS             complement(1562..2896)
                     /gene="purB"
                     /locus_tag="PLS16_05635"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNJ60596.1"
                     /translation="MTTLTAISPIDGRYRNKVADLENYFSEFALMKYRTHVEIEYFIY
                     LSNVIEQIGALSATQIQSLRTIVKDFSTEHALRIKEIEKTTNHDVKAVEYFIKENIKD
                     EDLKQKSEFIHFGLTSQDINNTSIPLMLKDFYHQVYVNKFKQLVDALTQKVQDYNAIP
                     LLARTHGQPASPTKLGKELQVFVERLTLQSNYFSTIPYSSKFGGATGNFNAHLVAFPA
                     IDWKSEANNFVENSLGLVRSQTTTQIEHYDNMAALFHNYSRINTILIDLCRDIWTYIS
                     MDYFKQKIKAGEVGSSAMPHKVNPIDFENAEGNLGIANALFYHLAEKLPISRLQRDLT
                     DSTVFRNVGVPLAHTLIAIDSILKGLDKLLVNEDKIRKDLEDNWIVVAEAIQTILRRE
                     GYEQPYEKLRDLTRTNAKIDDKVIHQFIDSLDVSELIKIELKQITPFNYTGI"
     gene            complement(2975..4723)
                     /locus_tag="PLS16_05640"
     CDS             complement(2975..4723)
                     /locus_tag="PLS16_05640"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNJ60597.1"
                     /translation="MIRKIGLFIMILFCTYSFAKPIIIDSHTKYISLNKKVFATTFEN
                     DTIVQKFEIYNNSNEEQEVFLEIPVENIQFLSIHSIFGVDIFNNERIRDRTLYDRDIV
                     FEYNFSPKSKVIFAVKYLRTDKNKNTEIVLWNKISRIINHQAVDLSRGFFYGFLIVFL
                     LICFVLFLLIREINYLRFFLFLFSGTLYLAVKHNLAYEFLWSKFPTIDYFVKKIALLL
                     YLIFGLNFFRSFIIKRLNDFSLDKYIKLVIFIGVLSIFISLGTGFYTPIIRDIFYLFQ
                     YFYIVICVLLIITSFYIAYFKLNDKSVFLFSIAFFASYAFFLFYPVPSSSRYFDEINL
                     KLIFPYLNTFIIFLLISFTVIWKVRQVVKDNKAMRKEIGKINAQQIFGAVTAQLSERK
                     RVGKELHDGIGILMAMAKMKLSSIKTKDNTEKKTLEGIIQQLDSTTQKIRKFSHDLLP
                     PTLIKFGLYIAINDEIDHYNEVKPNPIRFHADDTIKNLDEVSSYIIYNICINMIKYFY
                     GANNYNITIAIFISEYNKHCDIQINYAGNRINTKHRNIINIEKLIGLLQGSSSDELIN
                     TFTYKLDLDFPIRLKN"
     gene            complement(4720..6483)
                     /locus_tag="PLS16_05645"
     CDS             complement(4720..6483)
                     /locus_tag="PLS16_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60598.1"
                     /translation="MFNCNNILFTKEIILNDNSTELLLYKNSIGANELYKNNIELDFV
                     IQNKGKQLSYYLVGINPILDSLEIKFNHEIFYNGDKVSSENKRIKSYNSVEYLKFPRD
                     SILNLKVQIKTQQEQLNYRLLLVSQNQFLKIINRDSLLFGFFIGMFSMFLLLLISMYL
                     FSSNVFFLKYVQLNIITIAIYFYFSGIGYQYIWGKLNWVQSVLPTILFVFYTLVHASF
                     VKGFFNVKIEFPKLNILLNAYKFILFAFVVYTIIRLAFFFSDYRYFNWFLNIIYLMFV
                     IYVGLSFYIAFYTYYKTRRRESLWVIIGMIIQAFGWLVFFNTLYLSIPAFNAISRFQF
                     FESYLFISQFNFSMYVLEFILITFFIVINYKKIIMLNDVSYNRLVFLQQRNLKDYLKN
                     IENTRLSINNYLEKNILKEIKSIKSQFEEFIFFKEISKLNTLIIKDFDNVENDLTRIM
                     NDNNIKGLVGITLVDLIEHIFAQTVWNIKHKISYSDDVQNLQCNEIFNNHIYRIFQEL
                     MNNAIKHGAANEIDIQLLIENKKLIIVFKDNGISANNSDSKGLGILNIENRLKEFNGT
                     MEISKDNNWKVTMYILLKNII"
     gene            6546..8294
                     /gene="aceK"
                     /locus_tag="PLS16_05650"
     CDS             6546..8294
                     /gene="aceK"
                     /locus_tag="PLS16_05650"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF002804.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HNJ60599.1"
                     /translation="MINDTIINDVANHICDAYDNLLAKFNEITSTAKHDFETKQYALS
                     FSRKQDRYKLYGNYAKETLDYTKLHLGDSLYDIKVWKKIKEKYSDIIINKPYRLNSET
                     FYNSITRKVFVDKSFNPEIEYFDKDDYKPSENKENIIVHDTISVSSLSPLAIKQILQH
                     FKFDVPFENIEQDAINIFEELAPAFIYQKSNLHLDRIEIIKYVFYRNRGAFIIGRFRY
                     RKWRMPFVIPLLNEDNGIYADSIIHSEAEISIIFSFTRASFMVNTPYPAQLIDYLKRI
                     LPYKGVGELYDSIGYYKHGKTILYRDCMNYVLNEDHGDKFIIAPGIKGMVMCVFTLKY
                     YNFVFKLIKDKFEPPKNTTREAVIKKYEEVELSDRVGRMAYAHKFENLEFPRKLFSFE
                     LIQEFQKHCTESIEFKDDKVIIKHVYLERKMIPLNIYLKEASPIEAYKAILDYGYCIK
                     ELAAVNIFPGDLLLKNFGVTRHGRVIFYDYDEIEKITDLRFRKLPEISEEDERFGEMH
                     VSADLNDIFPEEFKGFMAPDGPMGDIFMAEHSELFDPKYWRKIQKKVAEGEYLRFFVY
                     NKNQRFRKGIKLDNDI"
     gene            complement(8445..8909)
                     /locus_tag="PLS16_05655"
     CDS             complement(8445..8909)
                     /locus_tag="PLS16_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60600.1"
                     /translation="MKTTFKTLSTLLLLIIAISAISCNKDKNGKDDDTGKTGKFTIDG
                     VEYSGNSEEQTFVNDNYSIVCQQDEPFKLIQITFHNKAEAITGGTFDVADFSLNVPSG
                     SVEVGVDGLTFDPDGAKTIVVAGNKISISNLPLIQTGGGSKHPLINSASINF"
     gene            complement(8962..9393)
                     /locus_tag="PLS16_05660"
     CDS             complement(8962..9393)
                     /locus_tag="PLS16_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60601.1"
                     /translation="MKTINKIFASGLLLIFISLVSSCSDGIFNVHEQYKLVFWFNQSS
                     SRTFLDDGVDSLTIYVNYQSVAKTPVDDVYWASSPTCDDKTAITATISLKPSQAEKVI
                     ISVKDQTGFEYQSSVVNLNSTLENNYCNSFEIVPSEVKIVH"
     gene            complement(9418..10347)
                     /locus_tag="PLS16_05665"
     CDS             complement(9418..10347)
                     /locus_tag="PLS16_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60602.1"
                     /translation="MKTILNLLSILLISTILFSCKKESVKSAVNLKGKIAPSLSMEKD
                     GEIEFFDLRNVNNPQKAAFNIIGTAENFYLQYVKSRPIGSKTIKTNYGDLSINHNKEM
                     YLADKNWPGWKNISTVGDWLIMNNDNTNQLYPAYQDYDDSTELYSYTNFFKDFASKPN
                     WGNAGTINGCAEVYENYNSILSTRNAFFFNFKTQEYLFDYNINNSGGIYTGYKITDLV
                     KKPNGNMDTDPIDWSNADFVIALSYNHSMQTLKYKGKNVTALVFVDMDTKMYAYFLRN
                     AEDDAVNGADKGRQTLITTSWQPISKLFKGWDL"
     gene            complement(10486..11940)
                     /locus_tag="PLS16_05670"
     CDS             complement(10486..11940)
                     /locus_tag="PLS16_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60603.1"
                     /translation="MKGNSIIFLISKIQKIYTKEIDTYLNSTKKQRTYKFYKLIVSAK
                     NDEQLEKSLLFRKLFNKTYSEKLDYLWRNEIRILKEELENFLITIEHQHISNHNNAYN
                     DWLLVQAFNRLQYIDGIDEKTEKLVKQKDNYASYNFALDACFIRVENVRYKITDISKR
                     LQTYPSLFQDCTILLDDLISAKCAELNLYKAQSNWISYNHHQEHRESLVDEDYICKLR
                     KNALSNLYNHFAASYTVHDNNDNKIQIKNLDQALEFIESIYQNNTLFQEVRINILIAK
                     GRELSANGFFKDSDIVLKSIKQDIDKQFKHHGIRTIYYVNYITNLVKCKYYDEVSNVL
                     DNDFSTDNMLYKNMLLYNRLLCYLYMRDTQNLSNYISYDLDAAPFPQNYMLKIIKSVY
                     FYLIREYDTAIIIINSLAQSVSSEKMLHYKNICQLFKKLYTVNQKYELQKQIPSKEIR
                     QIQEAIEEFENVTPNEFKLVSVYLWLKEEVTRIL"
     gene            complement(11953..12912)
                     /locus_tag="PLS16_05675"
     CDS             complement(11953..12912)
                     /locus_tag="PLS16_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60604.1"
                     /translation="MKRICLFTLFVAVLFTNIILAENQTFKPNSIHFNRLEQDTLDSF
                     IHKVGKDSVLHFLDNKIFQAKEILKKTVGAIKNEPTALELILLNKHFKLGLDSFLIFS
                     PNIVIENFSYVLYNERLLSNNYRYTSDFIKQQSQDSVSNLMYKNIHNNYGKISLIVYC
                     EQLHNIVNPKMIDSIYQTSLNYPINMRNDFYFLLIEYDKNCGRDQLKIKEFRKKLFEI
                     NYNNLNLGKVLSDTSEFNNLNYQNYKLMPSYQLLAYSLYAYDFDIKKHQKEVIYFLNS
                     QSKLGGFKEFNLAEFENISNPENMVYGLWSLLELREQIGKLYP"
     gene            12985..13725
                     /locus_tag="PLS16_05680"
     CDS             12985..13725
                     /locus_tag="PLS16_05680"
                     /inference="COORDINATES: protein motif:HMM:NF025175.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4184 family protein"
                     /protein_id="HNJ60605.1"
                     /translation="MPYTLAHPMFLLWSKRKFPKVSITAIIIASITPDFDILLRLTDT
                     RIHIFQYNLKDIFLLILPISFGIWLYYELCLKKIIVPIFPFLTTEDYKIKDFGLVLLT
                     MLLSILVHLFLDLISHWDAFSLSMMIGWNTGDPILTLLFYGYALYGNTILFSALGLFL
                     MTKYINFKTLLKFRPNKNQSIYFILLFIFTSIFFSFKIFMAVKEHKYFIDILIINFTG
                     ALVFAFLTTPIFFFILQKSKLIHAQPST"
     gene            13706..14542
                     /locus_tag="PLS16_05685"
     CDS             13706..14542
                     /locus_tag="PLS16_05685"
                     /inference="COORDINATES: protein motif:HMM:NF016377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ60606.1"
                     /translation="MPNQVHSLWIGNSLSAMELLTIQSFIENDFVFNLWVYEKINNLP
                     QGIILKDANEIISVHQIFKYKNQNKFGHGKGSVSGFSDLFRYKLLYDKGGIWTDMDIT
                     CLQSFEIKEPYFFRYHHQIGMVGNFMKVPAKSDLMWYCYQETLHQVNEDNINWLLPIE
                     ILIQGIKKYDLQNFVGNISNMDSFLVVRKLLSNKKTPITQWKIIHWMNEEFRRLNIDK
                     NTAIKNSTYQILLDKYHIKYQVLDKISALKLYFRISSFHYAWLNLKARINYFSNNFFL
                     RN"
     gene            14592..15971
                     /locus_tag="PLS16_05690"
     CDS             14592..15971
                     /locus_tag="PLS16_05690"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNJ60607.1"
                     /translation="MNSKSTKYIDKLKGLALIFILSAFSNFLYAGDCPKRYINPIFKE
                     VQYSAALKYGPYKKNTDGTHTWLIYDVYEPKNDTLSLRPVIILIHGGSFTSFPPIDRK
                     SPDIGGMAKALAERGYLVISPDYRLFRGEQTFEKMAETIFAASLDINELFCYLKNDVE
                     NGNQHKIDLNNLFFGGSSAGAILALNYGLFIDDISDLDFNPIYKNIMLKVAAFDGITD
                     ANSILHHKMCGIIPKGYLSISGALIDSNIIKPKIASVYQLHGKMDLALPYTTGYPLGN
                     PALPKLIGPGIFIDKFRAAGITVEADIYDDKFHVPVILPFGDNIPLAIELLLKTGDIY
                     DRPVLDSTIYRISHFCYDLMGKPSEQSCDSTLLFNKKNVLTNQLNLYPNPSNGIFNIE
                     IPKSLSNKANDITIYDSNGKIIFNTQTNLEANIKINLSTISKGMYWMILKSTDIKVKE
                     IYLQKVSIL"
     gene            16290..>17024
                     /locus_tag="PLS16_05695"
     CDS             16290..>17024
                     /locus_tag="PLS16_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNJ60608.1"
                     /translation="MDIQNQTVIPVENPINGKVLYEINDNPENVASYYKIADEVQQKI
                     QAMSIKERIAEVDKINQYVLDHSDFILDCIIAETGKARTDAFAAEVFEVGDVIDVYKK
                     LAPKLLKDKNIDMPVFLMGKKCKQYFEPLGTVLVITPWNFPFYQVIVPSLLSFLAGNA
                     TIAKPSEVTPLKPLWDHFFANSGFMKDAIQVVFGGKQVGAALIQQRPHKIHFTGSVKS
                     GKIIGKLAAEQLIPVDLELGGKDASIV"
BASE COUNT         6157 a         2487 c         2196 g         6184 t
ORIGIN      
        1 ggtttggttg ccatcacacc cgcagcgggt tatgtcagct acggaccaag ccttttcatc
       61 ggttttgcag catcgctcat tagtaactat gcagtggttg cattcaaaaa aacaagaatt
      121 gatgatactt tagatgtttt cccttgtcat ggcgttggtg gtatgatggg tatgattctt
      181 actggtgttt ttgctgctga ttatggaact ggaattacag attttggttt ggcttatgga
      241 caaacgaaaa cattttttgc tgaaattatt gcactaggaa gtgtcgctgc ttatgtattt
      301 tttggttctt atgctattta ttatattaca aataaaatta aaccaatcag agtaagtaaa
      361 actgatgaag agttaggttt agacatttct caacatggcg aaagttatac cttcgcaaaa
      421 attaatcttt aattgttaat tactaataca aaaacagagc tttagaaaac tctaaagctc
      481 tgtttttttt ttataaaatc taaacggcaa cctctccttt tatatgcgga tgagcttcgt
      541 aattttccaa tgtaaaatct tcatatttaa aatcaaaaat agacttaact tcaggattta
      601 ttttcatttt tggcaaagaa tatggtgttc tagtgatttg caatttcact tggtcaatat
      661 gattagaata aatatgagca tctccaaaag aatgtacaaa atcgcctaat cctaaaccac
      721 atacttgagc taccatcatt gtaaacaatg cataggacgc aatattaaaa ggaactccca
      781 aaaaagtatc ggcactacgt tggtataact gacaagacaa ttttccatca atcacataaa
      841 actggaaaat agtatggcaa ggtggtaatg tcatttgctc tatttctgcc acattccaag
      901 cacttacaat taaacgacgt gaatctggat ttgttttaat ttgatgaatt aagtttgtaa
      961 tttgatcaat ttcattacca tgctcatctt tccaatgtcg ccattgtttt ccataaacag
     1021 gtcccaattc tccatatttc aatgcaaatt catggtcagt cgcaattttt gcagcaaatt
     1081 ctttcatact ttccccacta aaatcagaac tacttttata tttctgataa ggccaatcat
     1141 tccaaatatt cactccattt ttacatagat attcgatatt ggaatcacct gcaataaacc
     1201 atagtaattc ataaacgatt gattttaaat gcaatttttt ggttgtaact aatggaaaac
     1261 catcgttcaa atcaaaacgc atttgatagc caaacacact aatagttcct gttcctgttc
     1321 tatcttcttt aatcgttcct ttatctaaaa tatgttgtaa aaaatcttga tattgtttca
     1381 tgtctataaa aataagcaac taattaataa acaaaatatt tcgatatgca cacctcgtta
     1441 gtttgtggag attaataacc aaaaagtata atgagtagta ttttaaataa tatttattat
     1501 aatattttcc aaagacaaaa aagcaatata aaatataatt tagaactatg aaaaatctta
     1561 gctaaattcc tgtataatta aaaggtgtaa tttgtttaag ttcaatttta ataagttcag
     1621 aaacatctaa actatctata aattgatgaa taactttatc atcgattttg gcattggttc
     1681 tagttaaatc tctcaacttt tcataaggtt gttcatatcc ttctcttctc aaaatggttt
     1741 gaatagcttc tgcaactaca atccagttat cttctaaatc ttttctaatt ttatcttcat
     1801 tcactaacaa cttatctaat ccttttaata tggaatcaat agcaatgagt gtatgtgcta
     1861 atgggacacc gacatttcta aaaaccgttg agtcggttaa atctctctgt aatcttgaaa
     1921 taggtaattt ctccgccaaa tgataaaata atgcatttgc aatacctaaa ttaccttcag
     1981 cattctcaaa atctattggg tttactttgt gtggcattgc actactgccc acctctcctg
     2041 ctttgatttt ttgtttgaaa taatccattg aaatatatgt ccaaatatct cggcataaat
     2101 caatcaaaat agtatttatt ctgctataat tatgaaacaa agctgccata ttatcataat
     2161 gctcaatttg agttgtcgtt tgtgagcgaa ccaatcccaa actattttca acaaaattat
     2221 tagcttcact tttccaatct attgctggaa atgcaactaa atgagcatta aaatttccag
     2281 ttgctccacc aaatttagaa ctataaggaa ttgtagaaaa gtaattggat tgtaaagtta
     2341 aacgttcaac aaaaacttgt aattccttac ctaatttagt tggagaagct ggttgtccat
     2401 gtgtacgagc cagaagcgga attgcattat aatcttgtac tttttgtgtt aatgcatcaa
     2461 ctaattgttt aaatttattt acataaacct gatgataaaa atcctttaac atcaaaggaa
     2521 ttgaagtatt attgatgtcc tgagaagtta atccaaaatg tataaattct gatttttgtt
     2581 tcaagtcttc atctttaata ttttctttga taaaatactc cactgctttt acatcgtgat
     2641 tggttgtctt ttcaatttct ttaattcgca aagcatgttc agttgaaaaa tcctttacaa
     2701 tagttcttaa agattgaatt tgcgttgcac tcaatgctcc tatttgttca ataacattag
     2761 ataaataaat gaaatattct atttcaacgt gtgttctata tttcatcaag gcaaattcag
     2821 aaaaataatt ttccaagtca gcaactttat ttcgatatct tccatctatt ggagaaattg
     2881 cagtaagcgt tgtcatttta tatacatttc aattcgtaca aaaataccaa atttgttact
     2941 tatctgcata tctaaacgat taaataaaaa acaactaatt ttttagtcga attggaaaat
     3001 ctaaatccag cttgtaagta aaggtattta ttaattcatc gctggagcta ccttgtaaca
     3061 atccaattaa tttttcaata tttataatat ttctatgttt agtattgatt ctatttcctg
     3121 cataatttat ttgaatatcg caatgcttat tatattcact aataaaaatt gcaatggtta
     3181 tattgtaatt attagcacca taaaaatact ttatcatatt aatacagata ttgtaaataa
     3241 tataactcga tacttcatct aggtttttga tagtatcatc tgcatgaaac ctgattggat
     3301 tgggttttac ttcattataa tgatcgattt catcattaat ggcaatatac aaaccaaatt
     3361 ttatcaatgt tggtggcaat aaatcatgtg aaaacttacg gattttttgt gtagtactat
     3421 ctaattgttg aatgatccct tccaatgtct ttttttcagt attgtccttt gtttttatgg
     3481 aagataattt catttttgcc attgccataa ggattccaat tccatcatgc aactcctttc
     3541 ctactctttt tcgctcgctt aattgtgcag taactgcacc aaatatttgc tgtgcattaa
     3601 tttttccaat ttctttacgc atggctttat tgtctttaac aacttgtcta actttccaaa
     3661 tcactgtaaa tgatatcaat aaaaatataa taaaagtatt caaataaggg aaaatcaatt
     3721 ttaaattgat ttcgtcaaaa tacctagatg aagaaggaac aggataaaaa aggaaaaatg
     3781 catacgaggc aaaaaaagct atagaaaaaa gaaaaacgga tttgtcattt agtttaaaat
     3841 atgcgatata aaatgaagta ataattaata atacacaaat gacgatataa aaatactgaa
     3901 acaaatagaa aatatctcta ataatcggtg tataaaatcc agtgcctaaa gaaataaata
     3961 tacttaaaac accaataaat atgaccaatt tgatatattt atctaaggaa aaatcattaa
     4021 gtcttttaat aataaatgac ctaaaaaaat taagtccaaa aattaaataa agcaacaatg
     4081 caattttttt aacaaaataa tctatcgttg gaaatttaga ccacaaaaac tcataagcta
     4141 aattgtgttt gactgctaaa tataatgtgc ctgaaaacaa aaagagaaaa aaacgtaaat
     4201 aattaatttc acgtattaat agaaataaaa caaaacaaat caataaaaac actatcaaaa
     4261 aaccataaaa aaaacctctg cttaaatcaa ctgcttggtg gtttataatt ctggatattt
     4321 tattccacaa gacaatttca gtatttttat ttttatcggt acgtaaatat ttgaccgcaa
     4381 atattacttt ggattttgga ctaaagttgt attcaaaaac tatatctcta tcatataatg
     4441 ttctatctcg aatacgttca ttgttaaaaa tatcaactcc aaaaattgaa tgtattgata
     4501 aaaactgaat attttctact ggaatttcca aaaacacttc ttgttcttca ttggaattat
     4561 tataaatttc aaatttttga actatggtat cattttcaaa agtcgttgcg aatactttct
     4621 tatttaaact aatatatttt gtgtgactat caataataat aggctttgca aacgaatatg
     4681 tgcaaaataa aatcattata aaaagcccta tttttctaat catataatat ttttgagtaa
     4741 aatatacatg gtaactttcc aattattatc cttactaatt tccatcgttc cattaaattc
     4801 tttcaatcta ttttcgatat tcaaaatacc caagccttta ctatctgaat tgtttgcact
     4861 gataccatta tctttaaaaa cgataattag ttttttgttt tctatcaata attgaatatc
     4921 aatttcgtta gctgctccat gttttatggc attattcatg agttcctgaa aaattcgata
     4981 aatatgatta ttgaaaattt cattacactg taaattttga acatcatcgg aataactaat
     5041 tttgtgttta atattccaaa ctgtttgagc aaaaatatgc tcaatcaaat cgaccaatgt
     5101 aattccaacc aaacctttga tattattgtc gttcattatt cttgtcaaat cattttcaac
     5161 attatcaaaa tcttttataa taagcgtatt caactttgaa atctctttaa agaaaataaa
     5221 ttcttcaaac tgagatttta ttgatttaat ttcttttaat atattttttt ccaaataatt
     5281 atttatactt aatctagtat tttcaatgtt tttaagataa tcttttaagt tacgttgttg
     5341 caaaaatacc aaccgattat aggaaacatc attcaacata attatctttt tataattgat
     5401 tacaataaaa aaagtaatca aaataaattc caaaacatac atactaaaat tgaattggga
     5461 gataaataaa taactttcaa aaaactgaaa tcggctgatg gcattaaaag ctggaatact
     5521 taaatacaaa gtattaaaaa ataccaacca gccaaaagct tgaataatca ttccaataat
     5581 tacccacaac gattctcgtc gtcttgtttt gtaataagta taaaaagcaa tataaaatga
     5641 tagtccaaca tagattacaa acattaaata aatgatattc aaaaaccaat taaaatatct
     5701 ataatcagag aagaaaaaag ccaagcgtat tatcgtataa acaacaaatg caaataatat
     5761 aaatttataa gcatttaaga gaatatttaa tttaggaaat tcaattttta cattaaaaaa
     5821 gccttttaca aaagatgcat gcactaaagt ataaaacaca aacaaaattg taggcaatac
     5881 actttgcacc caatttagtt taccccaaat atattgataa ccaataccag aaaaataaaa
     5941 ataaatggct atcgtaataa tgtttaattg gacatatttt agaaaaaaaa cgttactact
     6001 gaacaaatac atagaaatca aaagcaacaa aaacatcgaa aacattccaa taaagaaacc
     6061 aaaaagcaaa ctatctctat tgataatttt tagaaattga ttttgactaa ccaatagcaa
     6121 acgataattt aattgttctt gttgcgtttt tatttgaacc tttaaattaa ggatactatc
     6181 tctaggaaat tttaaatatt ctaccgaatt ataactttta attcttttgt tttcagaact
     6241 cactttatcg ccattataaa aaatttcatg attgaatttt atttccaaac tgtctaaaat
     6301 tggattaata ccaactaaat aataactcaa ttgttttcct ttattttgaa taacaaaatc
     6361 caattcgata ttatttttat ataattcgtt tgcaccaata ctatttttat acaataataa
     6421 ttcggttgag ttatcattca aaataatttc ttttgtaaaa agaatattat tacaattgaa
     6481 cattaagtac aataaaatga ttatttttat actattacga aaactcaatt ttatatattc
     6541 agataatgat aaatgataca ataattaatg atgttgccaa tcatatttgt gatgcttatg
     6601 ataatttatt ggctaaattt aatgaaatta cttcaactgc taaacatgat tttgaaacaa
     6661 aacaatatgc tttgtcattt tccagaaaac aagatcgcta taaattatat ggaaattacg
     6721 ctaaagagac tttagattat acaaaattac atttaggcga ttcactttat gacataaaag
     6781 tttggaaaaa aatcaaagaa aaatatagtg atatcatcat taataaacca tatcgtttaa
     6841 actcagaaac tttttataat tcaattacta gaaaagtttt tgtggacaaa agtttcaatc
     6901 cagaaattga gtattttgat aaagatgatt ataaaccctc tgaaaataaa gaaaatatta
     6961 ttgttcacga tacaatttca gtaagttcac tatctccgct tgcaattaag caaatattac
     7021 aacattttaa atttgatgtt ccatttgaaa atattgagca agatgccatt aatatttttg
     7081 aagagttagc accagctttt atttatcaaa aatcgaattt acatttagat agaattgaaa
     7141 ttattaagta tgttttttat agaaatcgtg gtgcatttat cataggtcgt tttcgatata
     7201 gaaaatggag aatgcctttt gtgattccat tattaaatga ggacaatgga atttacgctg
     7261 attctatcat tcacagcgaa gcagaaatta gtattatatt tagttttact cgtgcttcct
     7321 ttatggtcaa tacgccttat ccagctcagt taatagacta tttaaaacgt attttacctt
     7381 acaaaggtgt tggcgaattg tatgattcta tcggatatta caaacacgga aaaaccattc
     7441 tatatcgtga ttgcatgaat tatgttttga atgaagacca cggagataaa tttattattg
     7501 ctccaggtat taaaggaatg gtgatgtgtg tttttacctt aaagtattat aattttgttt
     7561 ttaagctcat taaagataag tttgaaccac cgaaaaacac aactcgagaa gctgtcatca
     7621 aaaaatatga agaagtagaa ctctccgacc gagttggtcg tatggcttat gcacataaat
     7681 ttgaaaactt agaatttcca agaaagcttt ttagttttga gcttattcaa gaattccaaa
     7741 aacattgtac ggaatccata gaatttaaag atgataaagt aataattaaa cacgtttatt
     7801 tagaacgtaa aatgatacca ttgaatattt atctaaaaga agcaagtccg attgaagcct
     7861 acaaagcaat tttagattat ggctattgta ttaaagaatt agcggcagtc aatatttttc
     7921 ctggtgattt attgttgaaa aactttggtg ttactagaca tggacgtgta attttttatg
     7981 attatgatga aattgaaaaa attacagatt tacgttttcg aaaattacca gaaatctctg
     8041 aagaagatga acgatttggc gaaatgcatg tttctgcaga tttgaatgat attttcccag
     8101 aagaatttaa aggatttatg gctccagatg gaccaatggg cgatatattt atggcagaac
     8161 acagtgaact ttttgatcca aaatattgga gaaaaattca gaaaaaagta gctgaaggtg
     8221 aatatttaag attctttgtt tataataaaa atcaaagatt tagaaaagga ataaaattag
     8281 acaatgacat ttaattgcat tcctattttt gttttctcat gtaatacaat taaaatgcta
     8341 aactaaaaaa gctattgtgc atctaatttt ggtatgatga tgtagatagt ttctaccaat
     8401 ctattacaaa cgatgcacaa tacctctttt acttttatta ttatttaaaa atttatgcta
     8461 gcggaattga ttaatggatg tttggaacca ccaccagttt gtattaacgg aagatttgaa
     8521 atagaaattt tatttcctgc aacaactata gtttttgctc cgtcaggatc gaaagttaga
     8581 ccatctacac ctacttcaac agagcctgat ggaacattta aactaaaatc tgcaacatca
     8641 aaagttccac cagttatagc ttctgcttta ttatgaaatg taatttggat taatttaaaa
     8701 ggctcatctt gttggcaaac tatagaataa ttatcattta caaatgtttg ttcttcagaa
     8761 tttcctgaat actcaacacc gtcaatagta aattttccag ttttaccagt atcatcatct
     8821 tttccatttt tatctttatt acatgaaatc gctgaaattg ctattattaa taaaagcaaa
     8881 gtagaaagtg ttttaaatgt agttttcatt cttttaaatt taatttatga gaaaatattg
     8941 attttaaatc ttagttgttt gtcaatgaac tatcttaacc tcagaaggca caatttcaaa
     9001 actattacaa tagttatttt ctaaagtact atttaaattt acaactgaac tttgatattc
     9061 aaacccagtt tggtctttaa ccgaaattat tactttttct gcttgactag gttttaaaga
     9121 aattgttgct gtaattgctg ttttatcatc acatgttgga gaacttgccc aatatacatc
     9181 atctactggt gttttggcaa ccgattgata atttacataa atagttagtg agtcaacacc
     9241 atcatctaaa aacgttcttg agcttgattg attaaaccaa aacactagtt tatactgttc
     9301 atgaacatta aagataccat cagaacaaga actcactaaa gagataaaaa tcaataataa
     9361 tccagatgcg aatattttat ttattgtttt cattttgttt atattttata tagaaaatta
     9421 caaatcccaa cctttaaata atttagaaat cggttgccaa gatgtagtaa ttaatgtttg
     9481 tcttcctttg tctgcaccat ttacagcatc atcttcagca ttccgcaaga aataggcata
     9541 cattttggta tccatatcta cgaatactaa tgctgtaacg tttttaccct tatactttag
     9601 tgtctgcata ctatgattat atgaaagtgc aataacaaaa tcggcgtttg accaatcgat
     9661 tggatctgta tccatattac catttggctt tttaactaaa tcagttattt tgtaaccagt
     9721 ataaatccct cctgaattat ttatattata atcgaaaaga tattcttgag ttttaaaatt
     9781 aaaaaagaat gcatttcttg ttgaaagaat tgaattataa ttttcataaa cttcagcaca
     9841 accattaatt gtaccagcat ttccccaatt aggtttagat gcaaaatctt taaaaaagtt
     9901 ggtgtatgaa tataattcag tagaatcatc ataatcttga taagcaggat aaagctgatt
     9961 ggtattatcg ttattcataa ttaaccaatc ccccactgtt gaaatatttt tccaacctgg
    10021 ccaattctta tctgccaaat acatttcttt attatgatta atagataaat ccccataatt
    10081 tgtctttatg gttttactgc caatcggtct gctttttaca tattgtaagt aaaaattttc
    10141 tgctgtcccg ataatattaa atgcagcttt ttgaggatta tttacatttc ttaagtcaaa
    10201 aaattcaatt tctccatctt tttccatact taacgatggt gcaatttttc cttttaaatt
    10261 tactgctgat tttacacttt cttttttaca agaaaataaa attgtactaa ttaaaaggat
    10321 gcttaataaa tttaatattg ttttcatttt tctctttttt tgttgatgta aaattgcacc
    10381 aaacagaaaa gtattaaaaa tgaaattttt aaattttgtg agaaataatt tttgtatatt
    10441 taatttaata cctttataat caatgaattg tcattttaaa gttaattata aaattctagt
    10501 aacctcttcc tttaaccata aataaactga aactaatttg aattcatttg gtgtaacatt
    10561 ttcaaattct tcaatagcct cttgtatttg tctaatttct tttgaaggta tttgtttttg
    10621 tagttcatac ttttgattta cagtatataa tttcttaaat aattgacaaa tatttttata
    10681 atggagcatt ttttctgaag aaacagattg tgccaaacta ttgatgatga ttattgctgt
    10741 atcatattct cgaattaaat aaaaatagac tgacttaata attttcaaca tataattttg
    10801 aggaaaaggt gcagcatcta aatcataaga aatatagtta gataaatttt gtgtatctct
    10861 catatacaaa tagcaaagca atctgttata caacaacata tttttataca acatattatc
    10921 ggtagaaaag tcattatcca gtacatttga aacctcatca tagtatttgc actttactaa
    10981 gtttgttatg taattcacat aataaatagt tcttattccg tgatgtttga attgcttatc
    11041 aatatcttgc ttgatacttt ttaaaacaat atccgaatct ttaaaaaagc cattagcaga
    11101 aagttctctg ccttttgcaa ttaaaatatt tattctaact tcttgaaata atgtattgtt
    11161 ttgataaata gattcaataa attctaatgc ttgatctaga ttttttattt gaattttatt
    11221 atcattatta tcatgtacag tataagatgc agcaaaatgg ttatataaat tacttaatgc
    11281 attttttctt agtttgcaaa tataatcttc atcgaccaaa ctttctctat gttcttgatg
    11341 atgattatag gaaatccaat tactttgtgc tttatataaa tttaattcag cacattttgc
    11401 cgatattaaa tcatctaata aaatagtaca atcttgaaat aaacttggat aggtctgaag
    11461 ccgtttacta atatcagtaa tcttatacct aacattttct actcgaataa agcaagcatc
    11521 taaagcaaaa ttatatgaag cataattatc tttttgttta actaattttt cagttttttc
    11581 atcaatccca tctatatatt gaagcctatt aaaagcttgt actaaaagcc agtcgttata
    11641 ggcattatta tgattagaaa tatgttgatg ttcaattgta atcaaaaaat tttccaattc
    11701 ttcttttaaa attcgaattt catttctcca taaataatcg agtttttctg aatatgtttt
    11761 attgaataat tttctaaaca acaaagattt ctctaattgc tcgtcatttt ttgcactgac
    11821 aattaattta taaaatttgt acgttctttg tttcttagta gaatttaagt aagtatcaat
    11881 ttctttagta tagatttttt gaatcttaga aattaaaaaa ataatagaat tgcctttcat
    11941 atttatacta atttatggat acaatttacc aatttgttct ctcaattcta ataaagacca
    12001 aagtccataa accatatttt ctgggttact aatattttca aattcagcta aattaaactc
    12061 cttaaaacct cctaactttg attgactatt caaaaaataa atgacttcct tttgatgttt
    12121 ctttatatca aaatcgtatg catataaact atatgccaat aactgataac ttggcatcaa
    12181 tttataattt tgataattaa ggttattgaa ttcactggta tccgaaagta ccttgcctaa
    12241 gttcaagtta ttataattta tttcaaacaa tttctttcta aattctttaa ttttaagttg
    12301 atctctaccg caatttttat catattcaat taataagaaa tagaaatcat tccgcatatt
    12361 aatcggataa tttaaagaag tttgataaat ggaatcaatc atttttggat tgacaatatt
    12421 gtgcaattgt tcgcaataaa caatcaaact aatttttcca taattgttgt gtatattttt
    12481 atacattaaa tttgagacag aatcttgact ttgttgtttt ataaaatcac ttgtatatct
    12541 ataattattg gagagcaatc tttcattata cagaacataa gaaaaatttt cgattacaat
    12601 atttggagaa aaaatcaaaa aagagtccaa gcccaattta aaatgcttgt ttaataaaat
    12661 taattccagt gcagttggtt cattttttat tgcaccaaca gtctttttca aaatttcttt
    12721 tgcttgaaaa attttattat ctaaaaaatg taacactgaa tcctttccta ctttatgaat
    12781 aaagctatcc aatgtatcct gttctaatcg attaaaatgt attgaattag gtttaaatgt
    12841 ttgattttct gctagaatta tattcgtaaa taagactgca acaaataaag taaataaaca
    12901 aattcttttc atacgattaa cttttttagt atgtttgtcc aaaataaaaa atactttaag
    12961 taaatatagc aatttgtaca atttatgcca tataccttag cacatccaat gtttttatta
    13021 tggagcaaac gtaagtttcc taaggtttcc attactgcaa ttatcattgc aagtatcacg
    13081 ccagattttg atatactgct tcgcttaacg gatactagaa ttcatatttt tcaatacaat
    13141 ttaaaagata ttttcctatt aattttacct attagttttg gtatttggtt gtattacgaa
    13201 ctttgtctaa aaaaaattat agtacctatt ttcccattct taacaactga agattataaa
    13261 attaaagatt ttggactagt tttattaacg atgttgcttt ctattctagt tcatctattt
    13321 ttagatttaa tttctcattg ggatgccttt agtctatcaa tgatgatagg ttggaatact
    13381 ggcgacccaa ttttaacttt actattttat ggctatgcac tttacggcaa tacaatactt
    13441 ttttctgcct tggggctatt tttaatgaca aaatacatca actttaaaac actactaaag
    13501 tttagaccta ataaaaatca atcgatttat tttatattgt tatttatttt tacaagtata
    13561 ttcttctcgt tcaaaatctt tatggctgtc aaggaacata aatattttat tgatattcta
    13621 attatcaatt ttacgggtgc cttagtattt gctttcctca caacgcctat ttttttcttt
    13681 attttacaaa aatcaaaact gatacatgcc caaccaagta catagtttgt ggataggaaa
    13741 ttcattatct gctatggaat tattgaccat tcaatcattt attgaaaatg attttgtttt
    13801 taatttgtgg gtgtatgaaa aaataaacaa tttaccacaa ggtattatac ttaaagacgc
    13861 caacgaaata atttcagtgc atcaaatttt taaatacaaa aatcaaaata aattcggaca
    13921 cggaaaaggg agtgtttcag gattttctga tttatttcgt tataaattat tatatgacaa
    13981 aggtggtatt tggacagata tggacataac ttgtttacaa agttttgaaa taaaagaacc
    14041 atatttcttt agatatcacc atcaaattgg aatggtcgga aattttatga aagtacctgc
    14101 aaaatctgat ttaatgtggt actgttatca agaaacttta catcaagtaa atgaagataa
    14161 tataaactgg cttttaccta ttgaaattct aattcaaggt ataaaaaaat atgaccttca
    14221 aaattttgtt ggaaatattt ctaatatgga tagtttttta gtcgtcagaa aacttctaag
    14281 caacaagaaa acaccgataa cacaatggaa aattatccat tggatgaacg aagaatttag
    14341 gcgactgaat atagataaaa atacggctat taaaaactca acttaccaaa ttcttttaga
    14401 caaataccat ataaaatatc aggttttaga taaaatatct gccttaaaac tttactttag
    14461 aatttcaagt tttcattatg cttggttgaa tttaaaagct agaattaatt acttttcaaa
    14521 taattttttt ctaagaaact aataataatt caaaataaat ttatattttc aatgcattaa
    14581 ttagtttgat aatgaattca aaatctacca aatacattga caagcttaaa ggattggctt
    14641 taatctttat tttatcagct ttttcaaact ttttatatgc aggagattgc ccaaaaagat
    14701 acattaatcc aattttcaaa gaggtacaat atagtgctgc actaaaatat ggaccttaca
    14761 aaaagaacac agatggcacg catacttggt taatttatga tgtatatgaa cccaaaaatg
    14821 acacactttc acttcggcct gtaattattc ttattcatgg cggtagtttt accagcttcc
    14881 cacctatcga tagaaaaagt ccagatattg gcggaatggc aaaagcctta gcagagcgtg
    14941 gttatcttgt catctctcct gattatcgtt tatttcgtgg cgaacaaact tttgagaaaa
    15001 tggctgaaac aatattcgca gcctctttag atataaatga gttgttttgt tatttaaaaa
    15061 atgatgttga aaatggaaat caacacaaga ttgatttgaa taatctcttt tttggaggct
    15121 cttcagctgg tgccatttta gcattaaatt atggtttgtt tatcgatgat atcagtgatt
    15181 tagatttcaa tccaatttat aaaaacatca tgctaaaagt agctgcattt gatggaatta
    15241 cagatgccaa ttcaatatta catcataaaa tgtgtggcat tatcccaaaa ggttacctat
    15301 caatttctgg agcattaatt gattcaaata ttatcaaacc caaaatcgct agtgtttatc
    15361 aattacacgg caaaatggat ttagcattac catacacaac tggctatcca ttgggcaatc
    15421 cagctcttcc aaaactaatt ggtccaggaa tatttataga taaatttaga gcagctggaa
    15481 taaccgtaga agctgatatt tacgatgata aattccacgt tccagtaatt cttcctttcg
    15541 gtgataatat tccattagct attgaattgc ttttaaaaac aggcgacatt tacgaccgac
    15601 cagttttaga tagtacaatt tatcgcatct ctcatttttg ttatgattta atgggcaaac
    15661 caagtgagca aagttgcgat tctaccttat tatttaataa aaaaaatgta cttacaaatc
    15721 aattaaattt atatccgaat ccttcaaatg gtatatttaa tatagaaatt cctaaatctc
    15781 ttagcaataa agcaaatgat attactattt atgattcaaa tgggaaaata atttttaata
    15841 ctcaaaccaa tttagaagct aatataaaaa ttaatttaag tacaatttct aaaggaatgt
    15901 attggatgat tttaaaatca acagatataa aagttaagga gatttatctt caaaaggtat
    15961 cgatattata atacactttt ataaattata aaggcttgaa tataatacaa taactttaac
    16021 taagcattca atttaattat ataagactga taagaaagtc aaacaacaaa aagaatctaa
    16081 gctttcattt aatttaaaca cggctatcgc attgataatg aggctcaaag caaaagaaca
    16141 atgaagaaaa ttgaaattta gtataaaatt aatgaataaa gaatttcttt caactgtctt
    16201 taaatgatat tattcaataa aaattatcct ttctttatag aattataatg aataaatacc
    16261 tattttagcc tacaaataaa taggtagaaa tggatattca aaatcaaacg gtcattcctg
    16321 tagaaaaccc aattaacgga aaagttttat atgagattaa tgacaatcct gaaaatgttg
    16381 caagttatta caagatagct gatgaagttc aacaaaaaat tcaagcaatg tctattaaag
    16441 aaagaattgc tgaagtggat aaaattaatc aatatgtatt agaccacagt gattttattt
    16501 tagattgcat tattgcagaa acaggcaaag ctcgtacgga tgcctttgct gcagaagtat
    16561 ttgaagtagg cgatgtaatt gatgtttata aaaaattagc tccaaaatta ttaaaagata
    16621 aaaatattga catgcctgtt tttttaatgg gtaaaaaatg taaacaatat tttgaaccat
    16681 taggaactgt tttagttatt acaccttgga atttcccatt ttatcaagtg atagttccta
    16741 gtttactatc ttttcttgct ggaaatgcga ccattgctaa accttcagag gttacaccat
    16801 taaaaccttt atgggatcat ttctttgcca actctggctt tatgaaagat gccattcaag
    16861 ttgtttttgg tggaaagcaa gttggagctg ctttaattca acaacgccca cataaaattc
    16921 acttcacagg ctctgtaaaa agtggaaaaa tcattggcaa attagctgca gaacaactaa
    16981 ttcctgttga tttagaattg ggtggaaaag atgcatctat tgtt
//
LOCUS       DAOQCW010000047        16985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1205_length_16985_cov_5.220319, whole genome shotgun sequence.
ACCESSION   DAOQCW010000047 DAOQCW010000000
VERSION     DAOQCW010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16985
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1205_length_16985_cov_5.220319"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..1136)
                     /locus_tag="PLS16_05700"
     CDS             complement(30..1136)
                     /locus_tag="PLS16_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60609.1"
                     /translation="MCSIELKAQSIYEQYQTIATSFGSDKKIPKLSIVNNDKIVAQFI
                     NGVEPKIQISKKAIAVCQSMKKDSSNALAIILSHELAHYYLNHADCFKYFAGNSIALN
                     NSSVLNEAEADRTGLVQTLKAGYSSTKIYQTLLDNVYTKFNLNENLRGYLSLEKRKEQ
                     ATQIAQEAEDHYRIFIAANFLTTTKNYVPAFIMYDFITSKYAFKENYNNAGVARLNTA
                     IELLLTNNPEKMPFLLPIELDALSRLSFSVERSDATTDNSERCKQLLLEANDKFSKAI
                     LLDKKYAFAYLNMACANILLGNPDAALGNIKDLEINGVKNKDSYTVKAIAYYSKGDKK
                     NAMSNFELAKKYNATNADYNLKLMKSKDSFWNFF"
     gene            complement(1504..1576)
                     /locus_tag="PLS16_05705"
     tRNA            complement(1504..1576)
                     /locus_tag="PLS16_05705"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1541..1543),aa:Arg,seq:tct)
     gene            1645..2352
                     /gene="ung"
                     /locus_tag="PLS16_05710"
     CDS             1645..2352
                     /gene="ung"
                     /locus_tag="PLS16_05710"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNJ60610.1"
                     /translation="MLNTEIQIEATWKLQLADEFQKSYFKNLIDFLAQEKAENRIFFP
                     KIVQIFNAYNFTPFDDVKVVIIGQDPYHGIGQAHGLCFSVQYGIKPPPSLVNIYKEIK
                     NNYPNFKIPNHGNLETWAKQGVLLLNSILTVEKNKPASHQKKGWEHFTDASISKLSQN
                     REGIIFILWGNFAQQKASLIDENRHHILKAAHPSPFSAHNGFFGCNHFEKVNQILRQQ
                     GKSEINWQLPVEPNLFG"
     gene            complement(2370..2906)
                     /gene="dcd"
                     /locus_tag="PLS16_05715"
     CDS             complement(2370..2906)
                     /gene="dcd"
                     /locus_tag="PLS16_05715"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNJ60611.1"
                     /translation="MILSDIEILKEIEKGNILIEPYDRKYLGTNSYDVHLSKYLAVYE
                     DKELDARKHNKIREILIPEEGFVLQPGTLYLGSTLEYTETLAHVPFLEGKSSMGRLGI
                     DIHATAGKGDVGFCNHWTLEISCIHPVRVYAGMPVGQLIYFCVQGAIENLYNKKSSAK
                     YTQRTSKPMESMMWKNNF"
     gene            complement(3005..3661)
                     /locus_tag="PLS16_05720"
     CDS             complement(3005..3661)
                     /locus_tag="PLS16_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60612.1"
                     /translation="MKKLFNRLAIFLAVILFTGACSKESNLKPTLEDRISQDVNLKKA
                     IDNATDLYTSLSREYVQTAAQNSELELLANKINNKTATAADYARVQQIVGMPYDEFIG
                     KVRNFALSLNDLAKSNPELAKMDAAQAQKVLTKAIDSNPELRSSLGKTLVINGKAEKC
                     PLRDICNLAVTLTKMFAGDAICAAINVTTIPVVGGLLCSLVLNLGAGLLTAICNALPC
                     "
     gene            complement(3802..6309)
                     /locus_tag="PLS16_05725"
     CDS             complement(3802..6309)
                     /locus_tag="PLS16_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ60613.1"
                     /translation="MYYRCIFLFLFLFFSFNKLSAQNSESINPKDAPAIGRVFGKVVD
                     ATNYQALSYATVFLSNLKDSTIQYANLVDDDGKFEISTIKIGAYKLIINYLGYKDYVI
                     EKLLIIPPDKINQDIGIIKVEEDSKVLQEVQIVGEKALMQLQADKKVFNVEKSALSAG
                     GSVVDVLRQTPTVDVDADGNISTRGSGNLQVYINGKPSGITGANKQAVLDAIPASAIE
                     SIEIMNNPNAKFDAEGEAGIINIILKKNYTKGINGSVYVGYRTVYKNSIGASINFRKK
                     KIGFTTSYNFKFHETHWSGYDRRKNINQDSSFFYLNTDNKGLQKHSYFNTISTQLDYD
                     INEKNTITIGSLFGSNWRENTGTTQYNFLDENQNTYNYFERYRHTKKFNWNVDANVYY
                     TKTFKSNKHNLIIASNYSISVDKDDPQYQQQAFLNDKTIDFNIPRSIENNKTKDRAHS
                     YQIQADYTQPFDKSKSLLEVGTKFTYRNLINDFYADSLNRATNMIILNNGLTNNFKYQ
                     EIVNAVYGVFSGAYKKFSYKTGLRMEQTNVFGNQSVGNLDFNRHYYDFFPSIFLAQEF
                     KNNHKLQFQYRRSINRPSAHQLNPFGEQQDPFNIRKGNPLLLPIYTNAVELSYVKNFK
                     KLFLTTTVYYRQSKNPYTMFRTVDSTGISIIDFGNLDKGRNIGTEIIARIQLTKWWNL
                     MANTNVFYNMLEGKVPNLEVSQDANSMTWNLRLISTMKVWKTGEIQLMWFYRGKNKFL
                     QGEIRPMSFFNIGFRKDFLKDNKASIAFNFTDVFRTQSFRIINKGANFEGNTKRNWDS
                     TIASITFTYKFGKQDSKPAQQQKKQDDNNQGIDGGGF"
     gene            complement(6309..6704)
                     /locus_tag="PLS16_05730"
     CDS             complement(6309..6704)
                     /locus_tag="PLS16_05730"
                     /inference="COORDINATES: protein motif:HMM:NF016156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HNJ60614.1"
                     /translation="MYKLFGLIASAYGIIAVILGAFGAHALKTKLDDYQHQIYDKAVH
                     YQFFHIVALMAVFLLSKFYPSKALNLSGWFFTMGTLLFSGSLYLLATRHLLGLDNFSK
                     VLGPVTPLGGLSFIVGWVFLFVAILKIKA"
     gene            complement(6784..8076)
                     /locus_tag="PLS16_05735"
     CDS             complement(6784..8076)
                     /locus_tag="PLS16_05735"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HNJ60615.1"
                     /translation="MHQFYTFSVDDISIFKEKVLKLSSIVNNFCYLDANNYTGYPYSS
                     FGSFFAIDAIHTITFSREDKNILQQFEVFRKAHQSWLFGFLSYELKNDIEYGTLYSNN
                     IDGIDNPVLNFFIPKYIIKIEDKKVELGVLNIDAVQEFETLLNQAEQIIKTASEVKIK
                     HRITKEQYVENVRLIQEQIRIGNVYEMNFCQEFYSENSKIDTLETFRKLNELSQAPFS
                     CYFKNNQNYLMCASPERFLKKSKQKLITQPIKGTRKRSINTIEDNNNKQELLSNEKEL
                     SENVMIVDLVRNDFSKIATKNSVQVEELFGLYSFKQVHQLISTISCQIGEEKSFKDII
                     EAMFPMGSMTGAPKLAAMQLAEKYEQTQRGMYSGAVGYITPKGNFDFNVVIRSILYNA
                     KNQYVSIQTGSAITIESIAEQEYEECLVKAQALFEALK"
     gene            complement(8091..9344)
                     /locus_tag="PLS16_05740"
     CDS             complement(8091..9344)
                     /locus_tag="PLS16_05740"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNJ60616.1"
                     /translation="MKVDVILGLQWGDEGKGKIVDFLADKYDVIARFQGGPNAGHTLV
                     IDGKKHVLHTIPSGIFRADKINFIGNGVVIDPVTLLKEIRTIEKEGIDVSKRLIIAKR
                     ANIILPTHKILDAASEASKGDSKIGSTLKGIGPTYMDKTGRNGLRMGDILETDFKVKY
                     ELLKNKHLQLLQIYDYNFDLKPMEEEWFAAIEELKKYQQEESVYFINNLLKDGKRILA
                     EGAQGSMLDIDFGTYPFVTSSNTITAAVCTGLGLAPSTIGKVIGITKAYCTRVGSGPF
                     PTELDNEVGEEIRKEGGEFGATTGRPRRCGWIDLPALKYTCLVNGVTDLVITKADVLE
                     AFYEFGVCTSYQYNGNTTQELPYDMINADIKPNFEYRPSWEHFSYTDKLPETLQSYVN
                     FIEEYLDTKVSMVSNGPEREAIIEL"
     gene            9567..9779
                     /locus_tag="PLS16_05745"
     CDS             9567..9779
                     /locus_tag="PLS16_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60617.1"
                     /translation="MLKVLKYIIIFLLGYKIVNELFGKKKQQTPLSNAPKKQRVETNN
                     YSQQSNPNNGQSTFDDAETIEYEELK"
     gene            9852..11519
                     /locus_tag="PLS16_05750"
     CDS             9852..11519
                     /locus_tag="PLS16_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60618.1"
                     /translation="MQFFKRNFLDILFLGFSLITLFLFFNHYKLYLDVSMADETVYMQ
                     RLNFKIWQLIGSFAPIYTLTYKFFHFFIKDYINLHHFAIISLSWVPSVGLYFFLRQKK
                     FPFIISAYLAWALFLSSFIVAFDWWPRVSHYSLFLIFIFFSWLPKYTKRFSKIFLLAA
                     CFALFMAFVRPEFFMSSMALFTLAFFTFLYEQYKNKITDKLLTVREKIFLVVFLICIA
                     LMTLAWHSPLYGGRMYYAMGQHYTFNTIKWNHKRDASDFIYWEQIFKNKFGNSETLSD
                     MYKANPKELQRHITENFYSYITQIFAFTTELFFPKFLFKIHFRIKWLLLIAIFLALLY
                     HTGIKKYKQVFLEKFQQHFIFIVCIMILVGASVVASIIIYPREHYYILQLAFIYYLFY
                     LLFIPFIENHTFTFKYQKLLNILILLSMIFATPNIKSYPRYNSFYHKYDTPVYLPYIY
                     AVRDLKIVKPTTFLVSEILPVYMGKNFRVNIQFLKDRPFDQLMQEQDIGMMYISDVLL
                     KDKRFSEDTTWIKFINNYESLGWVKLQLPDRKEYIIYRKDLLPNNAK"
     gene            11509..13047
                     /locus_tag="PLS16_05755"
     CDS             11509..13047
                     /locus_tag="PLS16_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60619.1"
                     /translation="MQNRFPFIFKLLILLAILAGFFLFVQMTVDDAYICFRYGYNLFH
                     YGIWNWNADHELIEAYTSFTYMMLSVIPPLIKVQPQIVFKIITFFFFLLIIRRIYLSI
                     HQKYWARLAILIYVSNWQTHVHIYAGLETTFWFWLLLEIFTLLDKNDFSNKIQTKLWL
                     FALLLPLTRPEGAIFSAFIFVYIALIKKEKIYFPTLFLFGSIGVLYFIARYLYFGLPL
                     PLSFYHKSVGNNLGIWGYVYNTFTIWQYLIGFFAIFFIAKSHKLFRYSALLSFGIYYV
                     FYGSSALLMNYANRFGFQIFYPIIIFGMIVIFDKLKSDKKIKQSLVAVSIFLILITAK
                     GLFSNNIVEISSIKNNAMFPFAMQKGHIKVGKAIKEIGHKDMKILLGDAGVIPYIGDC
                     KFYDAQGLADVYLSKQHINKAYFDSIKTDLVILVDFTNQVENLKGKTSTMGKIYDFIQ
                     AEKKHTYIGTLASMPSYNLFFYVRNDSKYKTEIEQQIQKAIIESNNYKFSSKDFIRMK
                     YLKI"
     gene            complement(13050..14948)
                     /gene="asnB"
                     /locus_tag="PLS16_05760"
     CDS             complement(13050..14948)
                     /gene="asnB"
                     /locus_tag="PLS16_05760"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNJ60620.1"
                     /translation="MCGIAGNFAFSTPNILENIWYKVAETLNKRGPDHQSITQINHTT
                     LVHARLSIIDTSSVAHQPIYDSTGRFAIIFNGEIFNFQKIKEDLVSKGYNSFKTQSDT
                     EVLLYAYIHYKEKCLDLLNGFFAFAVYDKLEDELFIANDRYAEKPLYYSQQDDGFYFA
                     SELKALMQLPIEKSLDWTAVSLYFQLNYIPNDISIFNKIKKLHAGHYIKVRSHKVELI
                     QWYHLPYSAEDAERNLLSYKQQQDKLRTLLDDAVRLRLIADVPIGAFLSGGIDSSVIV
                     SLASKHTPHLNTFSIGFEDNAYFDETEYANLVANHFKTNHTVFKLKNDDLLQSLQELL
                     DYTDEPFADSSALLVNLLSKYTKQHVTVALSGDGGDELFAGYNKHYGEWQSRNGGWKA
                     SVIQSLLPVWQKLPKSRSGGFGNKFRQLERFATGMNLSPAERYWRWCCFMDDDELNFL
                     LNGKPEAVLENIIKVRQQFTQFVHSDGTLNDCLRADVALVLANDMFVKVDRMSMRNSL
                     EVRAPFLDYRVVEYAFTLPVSSKIDAHLKKKIIQDTFRDVLPKELYNRPKKGFEVPLL
                     QWFRTDLKDYLDKEIFDEDFIKTQNIFNLEYIRSLKQQLFSNNPNDVHAKIWALIVFQ
                     NFYKKYIL"
     gene            complement(15022..>16985)
                     /locus_tag="PLS16_05765"
     CDS             complement(15022..>16985)
                     /locus_tag="PLS16_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60621.1"
                     /translation="KIQQLIQQNVAAVLPGQIATNLPIQATNTATSKIDSISSLLNLD
                     VRRTGLGEHKLYDAINDKNKATSFKGDVGIYYRPFKDKSYEFSYNYRIGFGNSVYQGT
                     DRYALRNFTQQFHKAEIKGKDFFIRSYLSQTNAGKSYNLTALGTYTNEGISGTKAVWA
                     PTYIANYAGVLIQNSILTSAANTLANAETLVAQAHQIARAAADKPWNDLTPEQQQALI
                     NKVRNGLFQQGGAGFIDHSRLFHTEGMVDLSRWTNKIVDIQIGGNHRMYSLFTNGTVF
                     NEDPDGTGHFKRINIQEYGAFLQLKRKLFKDALTLQGSIRFDKNQNFKGIWSPRIAAV
                     LSVGKDKMHNIRMSYQTGFRNPDTQAQYIYFPVASILLGGTEANAGRYGIYNGGAWTM
                     DSYKARQQALLSGKSLAYADSLLVTSDIGYVKPERLQAIELGYKAMFAKKFMIDINGY
                     FNIYDDFIGQIFVVNKDTAYHKGQAIAPGTTWYPYTNVASKVYSYGAGIGASYVLPKN
                     MLLKASYNWMDYTISKSNASGGSDEDLGFNAANHQFYVGISGDKVWKGLGFSVDYRWQ
                     SKINYLSSFASGIVKSRGILDANISYNIDKIFTQVKVGATNIAGPNYFSNIGGPLIGR
                     TFYVGLTFDQGKQWRENSRRIETEGNTSF"
BASE COUNT         5630 a         2818 c         2279 g         6258 t
ORIGIN      
        1 tttttgttca agctcgatta gttaataatc taaaaaaaat tccagaaact atctttagat
       61 ttcattagtt ttaagttata atcagcatta gtagcattgt atttttttgc tagctcaaaa
      121 ttactcattg catttttttt atcgcctttg ctataatagg caattgcttt tacagtataa
      181 gaatccttat ttttaacacc atttatttcc aagtctttta tatttcctaa tgcagcatca
      241 ggattgccta ataaaatatt cgcacatgcc atattcagat atgcgaatgc atatttttta
      301 tctagtaaaa tagctttcga aaatttgtca ttagcttcca ataataattg tttgcatctt
      361 tcactattat cagtagtagc atcgctcctt tccacagaaa aacttaaacg agaaagggca
      421 tcaagttcga tgggtaacaa aaaaggcatt ttttctggat tatttgtcaa caataactca
      481 atagcagtat ttaatctagc aactccagca ttgttatagt tttctttaaa agcatatttt
      541 gaagtaataa aatcgtacat tataaaagct ggaacataat tctttgtagt tgttaggaaa
      601 ttagcagcaa taaatattcg atagtgatct tctgcctctt gtgctatttg tgtagcttgc
      661 tcttttcttt tttctagaga taaatacccc ctcaagttct catttaagtt gaattttgta
      721 tagacattat ccaataatgt ctggtatatt tttgtagaac tataacctgc ttttaaggtt
      781 tgtactaaac cagtcctatc tgcttctgcc tcattaagta ctgaactatt atttagtgca
      841 atagaatttc ctgcaaaata tttaaaacaa tccgcatggt taagataata gtgtgccaat
      901 tcatgtgata aaataatagc caaagcatta gaggaatctt ttttcatact ttgacataca
      961 gcaattgctt tcttgctgat ttgtattttc ggttcaactc cattaataaa ttgtgctaca
     1021 atcttatcat tatttacaat acttagttta ggtattttct tatcgctacc aaaagaggta
     1081 gcaattgttt ggtattgttc ataaatgctc tgtgccttta attcaatact gcacataata
     1141 gatattacta aaagtgaaga tatagatatt attttgacag agaatttcat ttgaaacatt
     1201 tattaaagat agagataaag atattgcaag ataatatttt tcgattgaaa taagatatcc
     1261 ttcaattgta ggcttagtac tttaccaaac tacagcacca aaacatgcta tggttcagag
     1321 tactttcttc agtcatactt tttgtgtgct gtatgctaac gctcataaca cattgctttt
     1381 ttttagttat taatgtgtaa acctatttct ggacaagaca gtgatggagt ttcacttatt
     1441 tatttaaggt gatattaaaa aaattagggt ttcagcattt cactaaaacc cttgataact
     1501 aagtgatcgc ggcacgattc gaacgtgcga ccaactgctt agaaggcagt tgctctatcc
     1561 cctgagctac gcgacctacc ttgttttggt ttgcaaagat attaagtttt tagttaattt
     1621 tatacatttg attattaaaa aattatgttg aataccgaaa tacagataga agctacttgg
     1681 aaattacaat tagctgatga atttcaaaaa tcttatttta aaaacttaat agatttctta
     1741 gcgcaagaaa aagctgaaaa tcgtatattt ttcccaaaaa ttgtgcaaat ttttaatgcg
     1801 tataatttta caccattcga tgacgttaaa gtggttatta ttggacaaga cccatatcat
     1861 ggcattggac aagcacatgg tttgtgtttt tctgtgcaat atggtattaa acctccacca
     1921 tcattagtga atatctataa agaaataaaa aataattacc ctaattttaa aattccaaac
     1981 catggaaatt tagagacttg ggcaaagcaa ggtgtacttt tgttaaattc gatattaaca
     2041 gtcgagaaaa ataaaccagc ttctcatcaa aagaaaggtt gggagcattt tacagatgct
     2101 agtatttcca aattatcaca aaatagagaa ggtataatat ttatactttg gggaaatttc
     2161 gcacaacaaa aagcaagttt aattgatgaa aacagacatc atattttgaa agcagcacac
     2221 ccttcacctt tttcggcaca caatggattc tttggttgta accattttga aaaggtcaat
     2281 caaattttaa gacagcaagg aaaatctgaa atcaattggc aattacctgt tgaaccaaat
     2341 ttatttggat aagtttatac aatcaatctt taaaaattat tcttccacat catactttcc
     2401 ataggctttg atgtacgttg agtgtatttt gcacttgatt ttttattgta tagattttca
     2461 atagctcctt gaacacaaaa ataaatcaat tgtccaactg gcattcctgc atatactcga
     2521 actgggtgaa tacatgaaat ctctaaagtc caatgattac aaaagccgac atctcctttt
     2581 cctgctgtgg catgaatatc aatacctaaa cgtcccatac tggattttcc ttccaaaaaa
     2641 ggaacatgtg caagtgtttc agtatattct aaagttgatc ctaaataaag tgtacccggt
     2701 tgcaagacaa aaccctcttc tggaatgaga atttctctaa tcttattatg ctttctagca
     2761 tcgagttctt tatcttcata aacagctaag tatttagaca aatgtacatc ataagaatta
     2821 gtaccgagat attttctatc ataaggttct atgagaatat tacctttttc aatctctttc
     2881 aatatctcta tatctgataa aatcatgtgt aatttttgct acaaatttac tataatttac
     2941 tataaaaaaa caaagtcttt tccagaagga aaagactttg taatatattt agttttagaa
     3001 aaaattagca aggcaatgca ttgcaaattg cagtcaataa accagcacct aaattcaata
     3061 ctaaagaaca taataaacca ccaactacag gaattgtagt aacgttgatt gcagcacaga
     3121 tagcatcacc agcaaacatt tttgttaatg taactgctaa gttgcaaata tctcttaatg
     3181 gacatttttc tgcttttccg ttgataacta aagtttttcc taaactgctt cttaattctg
     3241 gatttgaatc aattgcttta gttaaaactt tttgagcttg tgcagcatcc attttagcta
     3301 attcaggatt tgatttagct aaatcattta aagaaagagc aaaatttctt acttttccaa
     3361 tgaactcatc ataaggcatt ccaacgattt gttgaactct agcataatca gcagccgttg
     3421 ctgttttgtt attgatttta tttgctaaaa gttctaactc actgttttgt gcagctgttt
     3481 gtacatactc tcttgacaag cttgtatata aatctgttgc attgtcaatc gcttttttca
     3541 agtttacatc ttgagagatt ctgtcttcta aagttggttt taaattactt tctttagaac
     3601 aagctcctgt aaaaaggatt acggcaagga aaattgccaa tctgttaaat aattttttca
     3661 tttttagtgt attaatttat taacaaagat aaaactattt tttaaataac caaatacggt
     3721 tacttatcat tagttaagtt tcaaaaatta aatacaatca aacaagacaa caatcatact
     3781 ttaggaaaaa gtttccatca attaaaaccc acctccatca attccttgat tattatcgtc
     3841 ttgctttttt tgttgctgtg ctggcttgct atcttgctta ccaaatttat aagtaaatgt
     3901 tatagaagca attgtactat cccaatttcg tttggtattt ccttcaaaat ttgcaccttt
     3961 atttataatt ctaaaacttt gtgttcggaa aacatccgta aaattaaatg ctatactagc
     4021 tttattatct ttcaagaaat cttttcggaa tcctatatta aagaaagaca ttggtctgat
     4081 ttcgccttgt agaaatttat tcttacctct ataaaaccac atgagctgaa tttctcctgt
     4141 tttccaaacc ttcatcgtac tgattaatcg caaattccaa gtcatactat tagcatcttg
     4201 cgatacttct aaattaggaa ctttaccttc caacatatta taaaatacat tggtatttgc
     4261 cattaaattc caccattttg tcaattgaat acgagcaata atttctgttc caatatttcg
     4321 ccctttatct aaattgccaa aatcaataat agatatacct gtactatcta cagttctaaa
     4381 catagtatat ggatttttag attgacgata ataaactgtt gtggttaaaa ataatttctt
     4441 aaaatttttc acataactta attcaactgc gtttgtataa attggcaata aaagtggatt
     4501 tcctttgcgt atattaaatg ggtcttgttg ttctccaaat ggatttaact gatgggcaga
     4561 tggacgatta atgcttcttc gatattgaaa ttgtaactta tgattatttt taaattcttg
     4621 tgctaaaaat atagatggga agaaatcata gtaatgacga ttaaagtcca aattcccaac
     4681 actttgattc ccaaacacat tcgtttgttc cattcttaat ccagttttgt aggaaaattt
     4741 cttataagca ccactaaata cgccataaac ggcattcaca atttcttgat acttaaaatt
     4801 attcgtcaaa ccattattta aaataatcat attggttgct ctattcagag aatcagcata
     4861 aaaatcatta attaaatttc tataagtaaa ttttgtacct acttctaata aagatttaga
     4921 tttatcaaat ggttgagtat aatctgcttg aatttgataa gaatgggctc tatcttttgt
     4981 tttattattt tctatagaac gaggaatgtt aaaatcaatg gttttgtcat tcaaaaaagc
     5041 ttgttgttgg tattgtgggt catctttatc tactgaaata gaataattgg aagcaataat
     5101 caaattatgc ttattagatt taaaagtttt agtataatat acatttgcat ctacattcca
     5161 attaaatttt ttagtatgtc tatatcgttc aaaataattg tatgtatttt gattttcatc
     5221 taagaagtta tattgtgttg tacctgtatt ctctctccaa ttagaaccaa ataaacttcc
     5281 aattgtaatg gtattctttt cattgatatc ataatccaac tgtgtactaa ttgtattaaa
     5341 ataactatgt ttttgcaacc ctttattatc cgtattcaaa taaaaaaagg aactatcttg
     5401 gttgatgttt tttcttctat catatccact ccaatgtgtt tcatgaaact taaaattata
     5461 agaagtcgtg aaaccaattt tcttttttct aaaattgata gatgcaccta tgctattttt
     5521 ataaactgtt cgataaccaa catacacact tccgtttatg cctttagtgt agtttttttt
     5581 gaggataata ttaataattc cagcttcacc ttcagcatca aatttggcat ttggattatt
     5641 cataatctca atactttcaa tagcacttgc tgggatagca tctaatactg cttgcttatt
     5701 tgctcctgta attcctgaag gttttccatt aatataaact tgtaaatttc ctgagcctcg
     5761 agtagaaata tttccgtcgg catcgacatc aacagttggc gtttgtctca atacatctac
     5821 aactgaaccg ccagcactta atgcactttt ttctacatta aatacttttt tatccgcctg
     5881 caattgcatc aatgcttttt cgccaacgat ttgtacttct tgcaacactt tgctgtcttc
     5941 ttccacttta attattccaa tatcttgatt gattttatca ggtggtataa tcaacaactt
     6001 ttcaatcaca taatctttat atcctaaata attgatgatt aatttataag caccaatttt
     6061 aatagttgag atttcaaatt ttccgtcatc atccaccaaa ttagcatatt gaatcgtgct
     6121 gtcttttaaa ttggaaagaa aaacagtagc gtaactcaag gcttgataat ttgtagcatc
     6181 tacaactttt ccaaaaactc gtccaatggc tggagcgtct tttgggttaa tgctttctga
     6241 attttgagca cttaatttat taaaagaaaa gaataaaaaa agaaatagaa aaatacacct
     6301 ataatacatt atgctttgat ttttagaata gccacaaaca aaaaaaccca cccgacaata
     6361 aaagacaatc ctcctaatgg tgttactgga cctaatactt ttgaaaaatt atcaagtccc
     6421 aataaatgcc gtgttgctaa taagtataaa gagccactaa ataacaaagt acccattgta
     6481 aaaaaccaac ctgataaatt caatgcttta gaaggataaa attttgacaa taagaatact
     6541 gccattaaag cgacaatatg aaaaaattga taatgcaccg ctttatcata aatttgatgt
     6601 tgataatcat ctaattttgt tttgagtgca tgagccccaa aagctcctaa aatcactgca
     6661 ataatgccat aagcagaagc aattagacca aataacttat acatatacta aattttagta
     6721 caaaggtatg attttaaata ctttttattg tgaaagtcta tttacgaaga ctgttaaaat
     6781 tagttatttc aaagcctcaa aaagagcttg agcctttacc aaacactctt catattcttg
     6841 ctctgcaata gattcaatcg ttatggcact tcctgtttgt atggacacat actgattttt
     6901 agcattgtat aaaatacttc ggataactac attaaaatca aaatttcctt taggtgtaat
     6961 atatccaacc gcaccagaat acatgcctct ttgtgtttgt tcgtattttt cagccaattg
     7021 catggctgcc aactttggag cacctgtcat gcttcccatc ggaaacatcg cctcaataat
     7081 atctttaaac gatttttctt ctccaatttg gcagcttatc gtagaaatta attgatgtac
     7141 ttgcttgaaa gaatataaac caaaaagttc ttctacttgt acactatttt tagtagcaat
     7201 ttttgaaaaa tcatttcgaa ccaaatctac aatcataaca ttctcactca actctttttc
     7261 attagacaat agctcttgtt tattgttatt atcttcaatg gtattaatac ttctttttct
     7321 tgtacctttt attggttgag taattagttt ttgctttgat tttttaagaa atctttctgg
     7381 acttgcacac atcaaataat tctggttgtt tttaaaataa caactgaatg gtgcttgtga
     7441 taattcattg agttttcgga atgtttccaa tgtatctatc tttgaatttt cggaataaaa
     7501 ctcttgacaa aaattcattt cataaacatt tccaattcta atttgctctt gaattagtcg
     7561 gacattttca acatattgct cttttgtaat tctatgtttt atcttgactt cagaggctgt
     7621 ttttataatt tgctctgctt gatttaataa tgtttcaaat tcttgaaccg catcaatatt
     7681 cagcacaccc aattctactt ttttatcttc aattttgatt atatattttg gtataaaaaa
     7741 attcaaaact ggattgtcaa ttccatcaat attattagaa tatagtgttc catattcaat
     7801 atcattcttt aattcataac ttaaaaagcc aaaaagccac gattgatgtg cttttctaaa
     7861 aacttcaaat tgttgtaata tatttttatc ttctctcgaa aatgtaatcg tatgaatagc
     7921 atctattgca aaaaaagagc caaaggaaga ataaggatat cccgtataat tattagcatc
     7981 taaataacaa aagttattta ctatgctaga caattttaaa actttctctt taaaaataga
     8041 aatatcatct actgaaaatg tataaaattg atgcattttc agtaattgtt ttacaattca
     8101 ataattgcct cacgctctgg tccgtttgaa accatactaa cctttgtatc taaatattcc
     8161 tcaataaaat ttacataaga ttgtagggtt tctggtaatt tatcggtata agaaaaatgc
     8221 tcccaagatg gacgatattc aaaatttggt tttatatcag cattaatcat atcataaggt
     8281 aattcttgtg ttgtattccc attatattga taacttgtac atacgccaaa ctcataaaat
     8341 gcttctaaaa catctgcttt tgtaataact aaatcagtaa caccatttac caagcaagta
     8401 tattttaagg ctggtaaatc tatccaacca caacgtcttg ggcgacctgt tgttgcacca
     8461 aattcgccac cttcttttct aatttcttca cccacttcat tatctaattc tgttgggaat
     8521 gggccacttc ctactcttgt acaataagct tttgtaattc caattacctt acctattgta
     8581 cttggagcta agcccaaacc tgtacaaaca gcggctgtaa tcgtattgga agatgttaca
     8641 aatgggtatg ttccgaaatc aatatccaac atagaacctt gagcgccttc tgctaaaatt
     8701 cttttcccat cttttaaaag attatttata aaataaacag actcttcttg ttgatatttt
     8761 tttaattctt caatcgctgc aaaccattct tcttccatag gttttaaatc aaagttataa
     8821 tcatagattt gtagtaattg caaatgctta ttttttagca attcatattt tactttaaaa
     8881 tctgtttcta aaatatcccc cattctcaaa ccatttcttc cagttttatc catataggtt
     8941 ggtccaattc ctttcaaagt agaaccaatt ttcgaatcgc cttttgaagc ttccgatgct
     9001 gcatctaaaa ttttatgtgt aggtaggata atatttgctc ttttggcaat aatcaatctt
     9061 ttgctgacat caataccttc tttttcaatt gttctaattt cttttaataa agtaactggg
     9121 tcaattacaa caccatttcc aataaaattg attttatctg ctctaaaaat tccagatgga
     9181 atggtatgca atacatgttt tttaccatca atcactaaag tatgaccagc attaggccca
     9241 ccttgaaatc ttgcaatcac atcatacttg tctgctaaaa aatcgacaat ttttccttta
     9301 ccttcatcgc cccactgcaa accaagaatt acatctactt tcattttctt tattttttga
     9361 aattactaaa gtctgaaatt gagaaattat ttcagtataa ccaattttta aacgtcattt
     9421 ttttgttatt ctaataaaaa gatacgagtt aaacacatat acatttgatt attagatatt
     9481 tatataaaaa attattaact taaataggta aatttatttt attaaattta acttttagat
     9541 tattctttat attctatttt tgtattatgc tcaaggtatt aaaatatatt attatctttc
     9601 tcttaggtta caaaattgtc aatgaattat ttggcaaaaa gaaacagcaa acacctttgt
     9661 cgaatgcacc taagaaacaa cgtgtagaaa ctaacaatta cagccaacag tccaatccaa
     9721 acaatggaca atcaacattt gatgatgctg aaacaattga gtatgaagag ctcaaataat
     9781 agatatattt agtatttcta ttacttattc attactaaaa atattgtatc ttagtacagt
     9841 ttttcgttaa gatgcaattt ttcaaaagga attttttaga tatattgttt ttaggctttt
     9901 ctctaattac acttttccta tttttcaatc attataaact ttatttagat gtttccatgg
     9961 ctgatgaaac cgtttatatg caaagattga attttaaaat ttggcaatta attggttcat
    10021 ttgctccgat atatacttta acttataaat tctttcattt cttcatcaaa gattatatca
    10081 acttacatca ttttgcaatc atttctttat cgtgggtgcc aagtgttggt ttatattttt
    10141 ttctacgaca aaagaagttt ccttttatta tttcagccta tttagcttgg gcattatttt
    10201 tatccagttt tatcgttgct tttgattggt ggccaagagt ttctcattat tctttattct
    10261 taatatttat attttttagt tggctaccaa aatacacaaa acgtttctca aaaatatttt
    10321 tattagccgc ttgttttgct ttgtttatgg cgtttgttcg acctgaattt tttatgagca
    10381 gtatggcgtt atttacatta gcgttcttta cttttttata tgagcaatat aaaaataaga
    10441 ttacggacaa gctactaaca gtgagagaaa aaatattttt agtagtattt ttaatttgta
    10501 tcgccttaat gactttagct tggcattctc cactctatgg aggcagaatg tattatgcca
    10561 tgggacaaca ttatactttt aatactataa aatggaatca taaaagagat gcctcagatt
    10621 ttatatattg ggaacaaatt tttaaaaata aatttggcaa tagcgagaca ttatcggata
    10681 tgtataaagc taatccaaaa gaattgcaaa gacatattac cgaaaatttc tattcctata
    10741 ttacccaaat atttgcgttt acaacagaat tattctttcc aaaattttta tttaaaattc
    10801 attttagaat aaaatggctt ttattaattg ctatattttt ggctctatta taccacactg
    10861 gaataaaaaa atacaaacaa gttttccttg aaaaattcca gcaacatttc attttcatag
    10921 tatgtattat gattttagta ggtgcttctg tggtggcatc tatcatcatt tatccgagag
    10981 aacattatta cattttgcaa ttagcattca tttattattt gttttattta ttgtttattc
    11041 catttattga aaaccatact tttactttca aatatcagaa gcttttaaat attttaattt
    11101 tattatctat gatttttgca acgccaaata ttaaaagcta tcctagatac aatagtttct
    11161 accacaaata tgatacgcct gtttatttgc catacatcta tgctgttcgt gatttaaaga
    11221 ttgtaaaacc cacaacattt ttagtcagtg aaattttgcc tgtttatatg ggtaaaaatt
    11281 ttagagttaa tattcagttt ttaaaagacc gtccattcga ccaattgatg caagaacaag
    11341 atattggtat gatgtatatt tctgatgttt tattaaaaga taagcgattt tctgaagata
    11401 ctacttggat taagttcatc aataattatg aaagcttagg ttgggtaaaa ttacaattac
    11461 ctgatagaaa agaatatatt atttacagaa aagatttact tccaaataat gcaaaatagg
    11521 tttcccttta tctttaagct actcattctc ttagcaatcc ttgctggctt ttttctattt
    11581 gttcagatga cagttgatga tgcatatatt tgctttcgat atggatacaa cttatttcat
    11641 tatggaatat ggaattggaa tgcagatcat gagcttattg aagcctatac aagttttact
    11701 tacatgatgc tttctgttat tcctccattg attaaagtac aacctcaaat tgtttttaaa
    11761 atcattactt tcttcttttt tttattgatt attagacgaa tttacctcag tattcatcaa
    11821 aaatattggg ctaggttggc tatcttaatt tatgtttcaa attggcaaac acatgttcat
    11881 atatatgcag gattagaaac cactttttgg ttttggttgc ttttagaaat ttttacccta
    11941 ttagataaaa atgatttctc caacaaaata caaaccaaac tttggctttt tgcattatta
    12001 ttaccgctta cacgtcctga aggtgcaatt ttctcagcat ttatttttgt gtacatcgca
    12061 ttaataaaaa aagagaaaat atactttcca acattattcc tatttggtag tattggtgta
    12121 ctatatttta tagctagata tttgtatttt ggcttaccat tacccctttc attctatcat
    12181 aaatctgttg gaaataattt aggaatttgg ggttatgtat ataatacatt tacgatttgg
    12241 caatatttaa taggcttttt tgcaattttt ttcattgcca aaagccataa actttttaga
    12301 tattctgctt tgctttcatt tgggatatac tatgtatttt atggctcttc tgcattatta
    12361 atgaattatg ccaatagatt tggatttcaa attttctatc caattataat cttcggaatg
    12421 attgtaattt ttgataaatt aaaatcagat aagaagataa aacagagttt agtggctgtt
    12481 tcaatatttc ttattttgat tactgcaaaa ggtctattca gtaataatat tgttgaaatt
    12541 tcatccataa agaacaatgc gatgtttccg tttgcgatgc aaaaaggaca cattaaagtt
    12601 ggcaaagcta taaaggaaat tggacacaaa gatatgaaaa ttttgttggg cgatgctggt
    12661 gtcattccat atattggcga ttgtaaattc tatgatgctc aaggtttagc agatgtttac
    12721 ctttctaaac aacatattaa caaagcctat tttgattcta taaaaacaga cttagttatt
    12781 ctggttgatt tcacaaatca agtagaaaac ttaaaaggaa aaacatcgac aatgggtaaa
    12841 atttacgatt ttattcaagc agaaaaaaaa catacttata ttggaacttt agcatctatg
    12901 ccgagttaca atctattttt ctatgtaaga aatgatagca aatataaaac tgaaatagaa
    12961 caacaaatcc aaaaagctat tattgagtct aataattata agttttcaag taaggatttt
    13021 attaggatga agtatctaaa aatatagtct tagagtatgt attttttata aaaattctga
    13081 aagacaatta atgcccaaat ttttgcgtga acatcattag gattattaga aaataattgt
    13141 tgttttaaac ttctaatata ttccaaatta aaaatatttt gggttttaat aaaatcctca
    13201 tcaaatatct ctttatccaa atagtctttt aagtcagttc taaaccactg taacaatggc
    13261 acttcaaaac cttttttggg tctattgtac aattcttttg gcaaaacatc tctaaaagta
    13321 tcttgaataa ttttcttttt taagtgagca tcaattttag atgaaactgg taatgtaaat
    13381 gcatactcca caactcgata atccaaaaat ggtgctctca cttccaatga atttcgcata
    13441 ctcattctat cgacttttac aaacatatca ttcgccaaaa ctaaagcaac atcagctctc
    13501 aaacaatcat ttagcgtacc atctgagtgt acaaattgtg taaattgctg tcgaacctta
    13561 ataatatttt ctaatacggc ttctggtttc ccattcagta aaaaatttaa ttcgtcatcg
    13621 tccataaaac aacaccaacg ccaatatcgt tctgctggac ttaaattcat tcctgtagca
    13681 aatctttcca attgcctgaa tttattaccg aaacctccac ttcttgactt cggcaatttt
    13741 tgccacacag gtaatagaga ttgaataaca gaagctttcc aacctccatt tctactttgc
    13801 cattcgccat aatgcttatt ataaccagca aataattcat ctccaccatc gccagaaaga
    13861 gcaactgtaa catgttgctt ggtatattta gacaacaaat ttaccaacaa ggcagatgaa
    13921 tctgcaaatg gctcatcagt ataatccaaa agctcttgta agctctgtaa taagtcatca
    13981 tttttaagtt taaaaacagt atgattggtt ttaaaatgat tagccaccag attagcatat
    14041 tctgtttcat caaagtaagc attatcttca aagccaatgg agaaagtatt caaatgtgga
    14101 gtgtgctttg acgctaaaga aacaattaca gaagaatcta ttccgccact taaaaatgca
    14161 ccaataggca cgtcagcaat caaacgtaat cgcacagcat catctaataa tgttctcaat
    14221 ttatcttgtt gttgcttgta agaaagtaga tttctttcag catcttctgc actgtatggc
    14281 aaatgatacc attgtataag ttcaacttta tggctcctta ctttgatata atgtccggca
    14341 tggagttttt taatcttatt aaaaatcgaa atgtcattag gaatataatt caattgaaaa
    14401 tagagcgaaa cagccgtcca gtctaaggat ttttcaattg gtaattgcat gagagcttta
    14461 agttctgatg caaaataaaa tccatcatct tgttgagaat aataaagtgg cttttcggca
    14521 tatctatcat ttgcaataaa aagttcatct tctaatttat cataaacggc aaatgcaaaa
    14581 aatccattca gcaaatccaa acatttttct ttataatgga tataggcata caataaaact
    14641 tcagtatctg attgtgtttt gaaagaatta tatcctttgg aaaccaaatc ttctttgatt
    14701 ttttgaaaat taaaaatttc accattaaaa ataatagcaa atcttccagt gctatcataa
    14761 attggttgat gagcaacaga actcgtatct attattgata aacgagcatg aactaaagtt
    14821 gtatggttta tttgtgtgat tgattgatgg tcaggacctc ttttatttaa tgtttctgct
    14881 actttatacc aaatattttc caaaatattt ggtgtggaga atgcaaaatt tccagctata
    14941 ccacacatac tctgcaaggt acaaaaaagt atggcaactc taaagagttg ccatactttt
    15001 ttttactatt ttctttatga attagaaaga agtatttcct tctgtttcaa ttcttcttga
    15061 attttctctc cattgttttc cttgatcaaa agttaaacca acatagaaag ttcttccaat
    15121 taatggacca ccaatattac tgaaataatt tggacctgca atatttgttg caccaacttt
    15181 tacttgagta aatattttat caatattata agatatattt gcatctaaga ttcctctaga
    15241 tttaacaatt ccactagcaa aacttgataa ataatttatc ttagactgcc atctgtaatc
    15301 aactgaaaaa cctaaacctt tccatacttt atctccacta attccaacat aaaattggtg
    15361 attagctgca ttaaatccta aatcttcatc agagccaccg cttgcatttg atttagagat
    15421 ggtataatcc atccagttat aacttgcttt aagcaacata ttttttggta atacataaga
    15481 agctccgata cctgctccat aagaataaac tttacttgcc acatttgtat atggatacca
    15541 agtagttcct ggtgcaattg cttgtccttt atggtatgct gtatctttat ttacaacaaa
    15601 tatttgtcca ataaaatcat cataaatatt aaaataacca ttaatatcaa tcataaattt
    15661 cttagcaaac atagctttat aacctaactc aatagcctgt aatctttcag gttttacata
    15721 tccaatatct gaagtaacca ataatgaatc agcataagca agtgatttac cagacaataa
    15781 agcttgttgt ctagctttat aagaatccat agtccatgca ccaccattat agataccgta
    15841 tcttccagca tttgcttctg ttcctcctaa taagatagat gcaacaggga aataaatata
    15901 ttgtgcttga gtatctggat ttctgaaacc agtttgataa gacatacgaa tattatgcat
    15961 tttgtcttta cctactgata acacagcagc aatacgtggc gaccaaatcc ctttaaagtt
    16021 ttgattttta tcgaaacgaa ttgatccttg taatgttaat gcatctttga ataattttct
    16081 ttttaattgc aagaatgcac catattcttg aatatttatt cttttaaaat gacctgttcc
    16141 atctggatct tcattaaata cagtaccatt cgtaaataaa ctatacattc tatgatttcc
    16201 accaatctga atatctacaa tcttatttgt ccaacgagat aaatctacca ttccttctgt
    16261 atggaacaaa cgagaatgat caataaaccc tgctccgcct tgttggaata atccatttct
    16321 aactttatta attaaagctt gttgttgttc tggagttaaa tcattccaag gtttatcagc
    16381 agctgctctc gcaatttggt gagcttgtgc taccaatgtt tcagcatttg ccaatgtatt
    16441 tgcagctgaa gttaaaatgc tattttgaat taatacacct gcataatttg caatatatgt
    16501 tggtgcccat actgctttcg taccacttat tccttcatta gtgtatgtac ctaaagctgt
    16561 caagttgtat gattttccag cattagtttg agacaaataa gaacgaataa agaaatcctt
    16621 tcctttaatt tctgctttat ggaattgttg ggtaaagttt cttagtgcat atctatcagt
    16681 accttgataa actgaatttc caaagcctat tctatagttg taagaaaact cataagactt
    16741 atctttaaat ggtctatagt aaatacctac atcaccctta aatgaagttg ccttattttt
    16801 atcattaatt gcatcatata acttatgttc gcctaaacca gttcttctaa catctaaatt
    16861 caataaagaa cttatagaat caattttaga agttgcagta ttagtagcct gaataggtaa
    16921 attagttgca atttgtccag gtaacacagc agctacattt tgttgaatta actgttgtat
    16981 tttaa
//
LOCUS       DAOQCW010000048        16964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1208_length_16964_cov_5.515524, whole genome shotgun sequence.
ACCESSION   DAOQCW010000048 DAOQCW010000000
VERSION     DAOQCW010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16964
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1208_length_16964_cov_5.515524"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /gene="rfbC"
                     /locus_tag="PLS16_05770"
     CDS             <1..479
                     /gene="rfbC"
                     /locus_tag="PLS16_05770"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNJ60622.1"
                     /translation="FLENGLNFDFVQDNHSKSCAGVLRGLHYQNAPYAQTKLVRVTQG
                     SVIDVAVDLRVGSPSYLKSFSIELSESNKKQLLIPKGFAHGFVVLSETCEFLYKCDAL
                     YNKEADGGIYFNDASLDIDWGNIDLDNLIISDKDKNLPLVKDATFNFPFEKYTNTL"
     gene            476..1240
                     /gene="cysQ"
                     /locus_tag="PLS16_05775"
     CDS             476..1240
                     /gene="cysQ"
                     /locus_tag="PLS16_05775"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HNJ60623.1"
                     /translation="MIQEIIKIAEQAGNAILEIYHKDFSIEYKDDKSPLTDADKVSNQ
                     IIIDELKRLSPNIPIISEENKLTEYSERKNWPKCWLVDPIDGTKEFIKKNGEFTVNIA
                     LIENGEPILSVLGVPAQNKIFYAEKGNGSFVIENATTKALKINDKITDTIRIVGSRSH
                     QTPELLAYVEEQNSKFKNVEFVAAGSSLKFCLIAENKADVYPRLGPTMEWDTAAGQLI
                     ATEAGAEVLVWETKHPLAYNRENLLNPYFIVQPKGF"
     gene            1335..>3330
                     /locus_tag="PLS16_05780"
     CDS             1335..>3330
                     /locus_tag="PLS16_05780"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNJ60624.1"
                     /translation="MNNRILLLNLFLLLFLGINAQQSISDLSVTYSQNFNSLAKTGAT
                     SNTLPANWHINVSANPMVYKVDVGSSNSGGIYSYGVINDDDRALGSVASSNASAKNIM
                     YGMQFENNTINNIQSITVSYTGELWRLGSKNIDTLFFQYNINADSIYKGTWVSVPQLN
                     FTTPDTTGVNGSRVGNDAPYKKNISYTITGLNISPSATFWIKWRDFDGLSSDDGLAID
                     DLSVSFAGVTLAACTTPTNNVSNVLLNSTITEIATSFTKASNSQKHLVVYSKNSTLSA
                     NPVDKANYSVGESLGGGTVAYIGNELNPNFIINKLIANTNYTLFFFPFNDSCSGGPLY
                     KVTSIISQTIKTKPFPNTSPYYVSLDTTLTCEQFKTALKDRISNGHTALQYNQIPEVF
                     GKTDMANGYIIDRYSTCQFTLLTNLGCPASSECNCYNREHLVPYSWFGTGENYPMYSD
                     MNFIYPSDAYVNSVQKENLPLGTTSTPYYTNGAGVKVGKSNINGYSGDVFEPGDQYKG
                     DFARAFLYFVTRYEDSLSVFKTRYASSPFTDSLFTGLEPWLLKTLVNWSKIDPPSSLE
                     ISRNDSVYQLQGNRNPYIDHPEWVEKAFGPNGNGCEVIIECNQPDTVATKLLFTNVTE
                     NSISGSFNKATGADGYVVIYRKGKSFLTTLTNNQTYTVGQM"
     assembly_gap    3331..3430
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3431..4369
                     /locus_tag="PLS16_05785"
     CDS             <3431..4369
                     /locus_tag="PLS16_05785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNJ60625.1"
                     /translation="KSFLTTLTNNQTYTVGQMITKTSGTITDTSYVGYVSANPNDTTF
                     NITGLVDNARYWIAVIPYKNCPSGKNYRSQFIDNVNKNDTTTANSSTTCDDPGQAPEG
                     LIFTSITKNSISGKFNKAVGGADAYLVLYRASSSFIVAPTDGQTYTVGQLITKVSGAY
                     TDSSTIGYISSGPNDTTFTINGLDAGKLYWIAVIPFNNCPSGPSYRTVIITNVNKNDT
                     STSSPNAIKINKELNFSVYPNPVSNNRLNIQLDKSNIDAVRVQIVDITGRILFNGNIP
                     EHTKVHQLEIANFGKGMYFLQLEDKNGLNTVPFLVQ"
     gene            complement(4421..5044)
                     /locus_tag="PLS16_05790"
     CDS             complement(4421..5044)
                     /locus_tag="PLS16_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNJ60626.1"
                     /translation="MKIKICGMREPENIRQIATLQPDFMGFIFYEKSKRFINLEDFLQ
                     LDTDYIGINKVAVFVNESIKNIIRIATLGNFKYLQLHGDETPEFCEELKYLDFKIIKA
                     FGINEQFDFHKLNEYETVIDYFLFDTQSTEYGGSGKKFDWNLLDKYKLQTPIFLSGGL
                     EISDIETVQNYCKQLPYCLDFNSKLEDKPGLKNIDKSSKVIQLIRKK"
     gene            complement(5041..5976)
                     /gene="queG"
                     /locus_tag="PLS16_05795"
     CDS             complement(5041..5976)
                     /gene="queG"
                     /locus_tag="PLS16_05795"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNJ60627.1"
                     /translation="MSAAENNAQLIKSIAKELSFSYCGISKAAYLEDQAPVLESWLKN
                     NYNGKLGYMENNFDVRLNPKLLVDDAKIVVSLMYNYYTNTQLEHDDYKISKYAYGKDY
                     HDVIKKKLWLFLDRLKEKIGDINGRGFTDSAPILEGVWAKKSGLGWIGKHSILITKPQ
                     GSFFFLAELVLDIDASIDTPLDKDYCGTCTACIDACPTNAILDSGIINASKCISYLTI
                     ELKDEIIPTEFKNQMENYIFGCDICQEVCPWNRFSIAHQEPEFIPKEALLKMKKEDWE
                     DITEDIFKEIFKGSPVKRTKFKGLTRNINFIKNKL"
     gene            complement(6022..6555)
                     /locus_tag="PLS16_05800"
     CDS             complement(6022..6555)
                     /locus_tag="PLS16_05800"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HNJ60628.1"
                     /translation="MIIPKKVEAVLNQHLEMEANAVFNYLAVASWCEREGLEGCAKFY
                     RDQSDEEHTHFMKFFDYINDVGEHAIVPAVKQPKSKFKNIKEVCEYSLKGEQAVTKSI
                     YHIIKVAEEEGDKATIDFLRFFVDEQREEEVQFKRIIDRIKLIGDGPQSLYYIDKEFE
                     KILNQKIKNAAKENNAG"
     gene            complement(6781..7155)
                     /locus_tag="PLS16_05805"
     CDS             complement(6781..7155)
                     /locus_tag="PLS16_05805"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HNJ60629.1"
                     /translation="MELYIKEVGNKGRGVFCTEDLEEGDEIEICPVIVCPPKDTKHLN
                     KTTLYHYYFIWGEDGESAGIVLGYGAMYNHDYSPNARYEAYYDDNIFKVIARKKIPAN
                     TEITINYNYYPEDQTKLWFDVE"
     gene            complement(7174..8172)
                     /gene="fbp"
                     /locus_tag="PLS16_05810"
     CDS             complement(7174..8172)
                     /gene="fbp"
                     /locus_tag="PLS16_05810"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HNJ60630.1"
                     /translation="MTIKDRLVTLDEFIIEQQNKIEDATGELSGLLRDIGLAAKIVNR
                     DLRKAGLVDNILGDAEKTNIQGEEVKKLDEFANTILTKTLQNSGECAGIASEEEDEIV
                     VFENRKKAKYVVLFDPLDGSSNIDVNATVGTIFSVFKRVTKLGENCTLEDFLQAGEKQ
                     AAAGYVIYGSSTMLVYTTGDGVNGFTLEPSIGEFCLSHPNIKTKKHALTYSVNQANYH
                     SFSENIRKYIDYCMSSDGNKKLYSLRYIGSMVADFHRNLIKGGIYMYPATHKDKNGKL
                     RLMYECNPLSFVQEQAGGKGTDGVRRILEIIPTELHQRTPVFVGSKEMVEHILENQ"
     gene            8244..9497
                     /locus_tag="PLS16_05815"
     CDS             8244..9497
                     /locus_tag="PLS16_05815"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNJ60631.1"
                     /translation="MRVFKFGGASVRDAAGIKNVASIIQKYGDKNTLVVVSAVGKTTN
                     ALEEVVAAYFDASKGGKKTLEPVKNNHLEICAGLFNTDNPVYAHLEKVFSEIEQRFDT
                     TPSDNYNHIYDQIVSQGELLSSIILSAYLKEAGIDNNWLDVREIIKTDNTYREAVVDW
                     VKTGELVATKVVPMVNGSIVVTQGFLGSTSDSQTTTLGREGSDYTAAIFANLLNAENQ
                     TIWKDVPGVMNGDPRIFSDAVFLEQISYNEAVEMTFYGATVIHPKTIKPLQNKNIPLH
                     VRSFINPEGVGTIVGKEDQKSLPPIRIVKKNQALLTLTTKDFSFIQEDVLASIFRAFS
                     KVNLKLNMMQQEAISFEAVVDNQEDKIARLMKFLQDNFQIEIQEDLSILTLRHYNDAT
                     IVQNSAGKEVLLVQKTSTTYQSLIR"
     gene            9648..11534
                     /gene="rpsA"
                     /locus_tag="PLS16_05820"
     CDS             9648..11534
                     /gene="rpsA"
                     /locus_tag="PLS16_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNJ60632.1"
                     /translation="MTTEKENPNLENDAELVARNKEVVEVEDLNLEEIKKEVVQKEFD
                     WNINKRGANRYSKEEAAQLEQLYDNTFNQIENNEIITATVSAVTDSDVVLNVGFKSDG
                     LVPRSEFKDMPDIKVGDEVLVFVENKENKKGNLILSRKKAKLVDAWERIKKAKDTDEI
                     VKGKVISKTKGGLIVDLFGMETFLPGSQIDIKPITDYDQFVGKMMEFKVVKINEAIKN
                     AVVSHKALIESDIESQRLEIIGKLEKGQVLEGTIKNITDFGAFIDLGGIDGLLYITDI
                     SWGRINHPSEVLQMDQKIQVVVIEYDENKKRISLGLKQLQAHPWDNLPEGIVEGSKVK
                     GKIVNIEDYGAFLEIVPGVEGLIHVSEISWGNQPINSRDFFKLGDEHEALVVTIDKDE
                     RKMSVSLKQMEEDPWKNITTKYAVGTRHKGIVKNITPYGVFVELDDIVGGMIHISDLS
                     WTKRYNHPNEYTSVGSELDVTILEIDSDNRKIVLGHKQLEEDPWDTFENLFPVGSVHE
                     ATVLKREDKGAIVQLPYGIEAFAPTRHIKKEDGKLADVDEVLKFKVLEFERDDKRIIV
                     SHTRVIEDEKLEEKRKVDGDKRKETRKQKDALKDVNSKVEKSTLGDLGALADLKAKME
                     GGEK"
     gene            11847..14270
                     /gene="pheT"
                     /locus_tag="PLS16_05825"
     CDS             11847..14270
                     /gene="pheT"
                     /locus_tag="PLS16_05825"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNJ60633.1"
                     /translation="MEISLNWLRDYVDIPLSVEEISVLLTDIGLEVEGVEDYVSIKGG
                     LEGLVVGFVQEKEKHPDADKLSVCKVDIGNEVLQIVCGAPNVAAGQKVIVALVGTQIF
                     PTKGEPFLIKKAKIRGVESVGMICAEDEIGIGESHDGILVLDENTEIGIPVSSLFNVY
                     KDTIIEIGLTPNRSDAHSHIGVARDLMAAYNYRNNKNIKLKAIETYQDTHKIEQGFKV
                     SVEDKEDCPKYLGILLGNISIQESPIWLQNKLRAIGQKPINYIVDVTNFVMHELGQPL
                     HAFDLTAISGNEIIVKKVKENTPFITLDGSEIKLSGTELMICNAEEPMCIAGVYGGKK
                     SGVTENTKSIFLESAYFNPKSIRKTSTKHQLKTDAATHFEKGIDPNITQIALERAIFL
                     LQQIDSNITLLSNMYNIENEEFNHFEIKLNFNKVRQITGASITNEQILKILDLLEIKV
                     INLTDLGTDLLVPPYRADVQREVDVIEDILRIYGFNNILIPSKINASVNITETKNPEQ
                     IYEQVANDLTSIGYAEILTNPLSKSKLIEKFEPARENWVNLLSSINVELDVLRNNMLF
                     STLETVSYNNNHKNSNLRLFELGRTYHKNGQDYIEQEHLILCHFGLKQEPNWKATAQK
                     TDFFTLKTSVNRLLQKIGINQFEITFSESKYFDYGLEYAINDAIIVKFGKVSSNWTTL
                     FDIKQEVYFAEFNWLKLIELYNSNKVSYKPISKFPSVKRDLALLLNKEIKFEELKTVA
                     EKIGKKLLQKIDIFDIYTDDKMKANEQSYALSFIFKDEHKTLQDADIDSIMQKMIQVF
                     QKEFNATVR"
     gene            14270..14677
                     /locus_tag="PLS16_05830"
     CDS             14270..14677
                     /locus_tag="PLS16_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60634.1"
                     /translation="MSKLDDRWNRIVKKIHLIIEKNKKLEEVELTLRNKINELEKSNK
                     SLTEQNSELNKEEQQTEIINQQNYIKELEQQQEILNKNLEQIKIENQTLKEALKVKES
                     NAVIANVEEREALKKQIEQHINKIDDILELINP"
     gene            14677..14976
                     /locus_tag="PLS16_05835"
     CDS             14677..14976
                     /locus_tag="PLS16_05835"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNJ60635.1"
                     /translation="MAEQNNINIMVAGRPYKLKVSGEEESFVRQAAKEINDKISEFQK
                     TLLTRDKQDFLSMIALQTTADMLQSKNKITNSASLEAKFDELEQLLDRSLGITLF"
     gene            15055..16650
                     /gene="rny"
                     /locus_tag="PLS16_05840"
     CDS             15055..16650
                     /gene="rny"
                     /locus_tag="PLS16_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNJ60636.1"
                     /translation="MIEIVTGVVALIIGIVIGKFLFKANVDKQLQEAKLLEEKAQSMV
                     DNAKKESEIIVKEASTLAEATKKDKMLEAKEHFFKLKEEHNKEIDQRNKKTVETENRL
                     KQVEQSLKDRLANAGKQEKDNEEIKAKLQLQLEVMNAKQADLEKAHQEHISKLEQIAK
                     LSAEDAKAQLVEQLKGKAQTEAIAHVKMVMDEAKLKANKEAKKIIIQSIQRVAAENAI
                     ENAISVFNLQSDDQKGQIIGREGRNIRALEAATGVEIIVDDTPEAIVISGFDPYKREI
                     ARLSLQRLVTDGRIHPARIEEVVAKTEKMLEQQIMEIGERTVIDLGIHGLHPELIKIV
                     GKMRFRSSYGQNLLQHSREVSNLCAIMASELGLNAKMAKRAGLLHDIGKVSDEDPELS
                     HALLGAKLCERYGEHPAIVNAVGAHHDEMEMLFVISPIIQACDAISGARPGARREIME
                     SYMKRIAELEKLALNYNGVEKAYAIQAGRELRVIVEAGKTSDKDVEETAFKIAQQIQD
                     EMTYPGQIKVTVIRETRGVSVAK"
     gene            complement(16795..16868)
                     /locus_tag="PLS16_05845"
     tRNA            complement(16795..16868)
                     /locus_tag="PLS16_05845"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16832..16834),aa:Ala,seq:ggc)
BASE COUNT         6124 a         2306 c         2799 g         5635 t
ORIGIN      
        1 aatttttaga aaatggatta aattttgatt ttgtacagga taatcattca aaatcatgtg
       61 ctggagtatt acgaggtttg cattaccaga atgcacccta cgcacaaact aaattagtaa
      121 gagtaactca aggtagtgtt attgatgttg ctgttgattt acgtgtaggc tctccatctt
      181 atcttaaatc tttttccata gaattgtcag aaagtaataa aaaacaatta ttaataccaa
      241 aaggatttgc tcatggcttt gtagtacttt ctgaaacttg tgagttctta tacaaatgtg
      301 atgcattgta taataaagaa gcagatggtg gcatttattt taatgatgct tctttagata
      361 tagattgggg taatattgat ttagataatt taataattag tgataaggat aaaaatcttc
      421 cattagtaaa agatgctacc tttaatttcc cttttgaaaa atatacaaat actttatgat
      481 acaagaaata attaagattg ctgagcaagc agggaatgct attttggaaa tttaccacaa
      541 agatttttca attgaatata aagacgataa atcaccattg acagatgctg ataaagtttc
      601 taatcaaatt atcattgatg aattaaaaag attatctccc aatattccaa ttatttccga
      661 agaaaataaa cttacagaat attctgaaag aaaaaactgg ccaaaatgtt ggttagttga
      721 ccctattgat ggtaccaaag agtttattaa gaaaaatgga gaatttacag ttaatattgc
      781 tttaattgaa aatggtgaac caattttaag tgtcttaggt gtgcctgctc aaaacaaaat
      841 tttttatgca gaaaaaggaa atggttcttt tgtgattgaa aatgcgacta caaaagcatt
      901 aaaaataaat gataaaatta cggatacaat tagaattgtt ggtagtcgtt ctcaccaaac
      961 accagaatta ttagcttatg tagaagagca aaatagcaaa tttaaaaatg tagaatttgt
     1021 tgccgcaggt agttctctta aattttgttt gattgctgag aataaagctg acgtatatcc
     1081 acgcttaggt ccaacaatgg aatgggatac tgctgcaggg caacttatag ctactgaagc
     1141 tggtgcggaa gttttagttt gggaaaccaa gcacccttta gcttataata gagaaaattt
     1201 attgaacccg tattttattg tacagcctaa aggtttttaa gttgttagtt ttcttaatta
     1261 ttctttgaaa ataagtgcat aacttatttt caaagaataa tatttgtttt gtatttttga
     1321 gaaaattact gaatatgaat aatagaattt tactattaaa tctttttcta ctattgtttt
     1381 taggtataaa tgcacaacaa tctatatctg atttatctgt aacttattcc caaaacttta
     1441 atagtttagc taaaactggt gcaacttcta atactttacc agccaattgg catattaatg
     1501 tatcagccaa tccaatggtt tataaagtgg atgtaggatc ctcaaattct ggaggtattt
     1561 atagttatgg tgtcattaat gatgatgata gagcattagg ctcagtagct tctagtaatg
     1621 cttctgctaa aaacattatg tatggaatgc agtttgaaaa taatacgatt aataatattc
     1681 aatcaattac agtttcctat actggagagt tgtggagatt gggttctaaa aatatagata
     1741 ccttattttt tcaatataat attaatgcag acagcattta taaaggaact tgggttagtg
     1801 ttccacaatt gaacttcaca acacccgata caactggagt taatggaagt agagtgggta
     1861 atgatgctcc ctataaaaaa aatattagct atacgattac tggtttaaat atttctccaa
     1921 gtgcaacttt ttggattaaa tggagagatt ttgatggatt aagttctgat gatggtctag
     1981 cgattgatga tttatcagtt tcatttgctg gagttacttt agctgcttgt actactccaa
     2041 caaataatgt ttcaaacgtt cttttaaatt caacaattac agaaattgca actagtttta
     2101 cgaaagcgtc aaatagtcaa aaacacttag tggtttattc taaaaattca acattaagtg
     2161 ctaatcctgt tgataaagct aattattcag taggagagag tttaggaggt ggaactgttg
     2221 cttatatagg taatgaattg aatcctaatt ttattataaa taaattaatt gcaaatacaa
     2281 attacacgct tttctttttt ccttttaatg attcttgttc aggcggacct ttgtataaag
     2341 ttacatcaat tatttctcaa acgataaaaa caaagccatt tccaaatact tcgccttatt
     2401 atgtttcttt agatacaaca ttaacttgcg agcaatttaa aactgcacta aaagatagaa
     2461 tttctaatgg acatacagct ttgcaataca atcaaattcc tgaagtattt gggaaaactg
     2521 atatggcaaa tggatatatt attgaccgat actcaacatg tcaatttact ttattaacta
     2581 atttaggttg tccagcttct agcgaatgta attgttataa ccgtgaacat ttagttcctt
     2641 actcttggtt tggaactggt gaaaattatc caatgtactc agatatgaat tttatttacc
     2701 catctgatgc ttatgtaaat agtgttcaga aggaaaattt acctttaggt acaacaagta
     2761 caccatatta tacaaacgga gcaggtgtaa aagttggaaa atcaaatatt aatggttatt
     2821 caggtgatgt ttttgaacca ggagaccaat ataaaggcga ttttgctcgt gcatttttat
     2881 attttgttac tagatatgaa gatagcttga gtgtatttaa aactcgttat gcttcaagcc
     2941 catttacaga ttctttattt acaggattag agccttggtt attaaaaact ttagtaaatt
     3001 ggagtaaaat tgacccacca agttctttag aaatttcaag aaatgattct gtttatcaat
     3061 tacaaggtaa cagaaatcct tatattgacc atcctgaatg ggttgaaaaa gcatttggtc
     3121 caaatggaaa tggttgtgaa gtaattatag aatgtaatca accagatact gtagctacaa
     3181 aacttttatt tacaaatgta actgaaaatt caatttcagg ttcatttaat aaagcaactg
     3241 gtgcagatgg ttatgttgtt atttatagaa aagggaaatc tttcttgaca actttgacaa
     3301 ataaccaaac ttatacggtt gggcaaatga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn aaatctttct tgacaacttt gacaaataac caaacttata cggttgggca
     3481 aatgattact aaaacaagtg gcacaataac cgatacttct tatgttggtt atgtttctgc
     3541 aaatcctaat gatacaactt ttaatattac aggtttagta gataatgcac gttattggat
     3601 agctgtaatt ccttacaaaa attgtccttc aggaaaaaat tatcgttctc aatttattga
     3661 taacgtaaat aaaaatgata cgactactgc aaatagttca acaacttgtg atgatcctgg
     3721 acaagcacca gaaggtttga tatttactag tataactaaa aattcaatct caggtaaatt
     3781 caataaagct gttggtggag cagatgctta tttagtgctt tatagagctt cgagttcatt
     3841 tattgtagct cctacagacg gacaaactta tactgttgga cagttaatta ctaaagtaag
     3901 tggtgcttac acagatagtt caacgattgg ttatatttca tcaggaccaa acgatactac
     3961 ttttactata aatggattag acgcaggtaa attatattgg attgctgtta ttccatttaa
     4021 taattgccct tcaggtccaa gttatagaac tgttattatt acgaatgtca ataaaaatga
     4081 cacatcaaca tcttctccaa atgctataaa aattaataaa gaattgaatt tttctgttta
     4141 cccaaatcca gtaagtaata acagattaaa tattcaatta gataaaagta atattgatgc
     4201 agttcgagta caaattgtag atattactgg tcgtatttta tttaatggaa atattcctga
     4261 acatacaaaa gtacatcaat tagaaattgc taattttgga aaaggaatgt acttcttaca
     4321 attggaagat aaaaatggtt taaatactgt accgttttta gttcaataaa atattagaat
     4381 gtgtaataaa aattgtaaca gctcaaattt atttgggctg ttattttttt ctaatgagtt
     4441 gaattacttt cgatgattta tctatatttt ttagtcctgg tttgtcttcc aatttgctgt
     4501 taaaatctaa gcaataaggc aattgtttac aataattttg tacagtttca atatctgaaa
     4561 tttctaatcc tcctgataaa aatataggtg tttggagttt gtatttatct aataaattcc
     4621 aatcaaattt tttgccactg ccaccatatt cagtactttg tgtgtcaaat aaaaaataat
     4681 caatgacagt ctcatattca tttaatttat gaaaatcaaa ttgttcattg atgccaaacg
     4741 ctttaatgat tttaaaatcc aaatatttta attcttcaca aaattcaggg gtttcatctc
     4801 catgtaattg taagtattta aaattaccta gtgttgcaat tcttataata tttttaatgc
     4861 tttcatttac aaaaactgct actttattta tacctatata atcagtatcg agttgtaaaa
     4921 aatcttctaa atttataaaa cgttttgatt tttcatagaa aataaaaccc ataaaatcag
     4981 gctgtaaagt ggcaatttgc cttatgtttt ctggctctct cataccacaa atttttattt
     5041 tcatagcttg ttttttataa agttgatatt tctagttaat cctttaaatt tagttctttt
     5101 tactggagaa cccttgaaaa tttctttaaa aatatcttca gtaatatctt cccaatcttc
     5161 ttttttcatt ttcaataatg cttcttttgg aataaattca ggttcttgat gggcaattga
     5221 aaaacgattc caagggcaaa cttcttgaca aatatcacaa ccaaaaatat aattttccat
     5281 ctgattttta aactcagtag gaataatttc gtcttttagt tcgatagtca aataggatat
     5341 gcattttgaa gcatttataa ttccagagtc taatatggca tttgttggac aggcatctat
     5401 gcaagcggta catgtaccac aataatcttt atctaaaggt gtgtctatgc tggcatcaat
     5461 gtctaaaact aattcggcca aaaagaaaaa tgaaccttgt ggtttagtaa ttaaaatact
     5521 gtgtttccca atccaaccta atcctgattt tttagcccaa acaccttcta aaataggagc
     5581 ggagtcagta aatcctctac catttatgtc tccaattttt tcttttaaac gatctaaaaa
     5641 tagccacaat ttctttttga ttacatcgtg ataatcttta ccataagcat atttagaaat
     5701 tttataatcg tcatgttcta attgtgtatt tgtataataa ttgtacatta aggaaacaac
     5761 tatttttgca tcatcaacca gtaattttgg atttagacgc acatcaaaat tattttccat
     5821 gtaccctaat ttgccattgt aattattttt taaccaactt tccaagacgg gagcttggtc
     5881 ttctaaataa gcagctttgc tgatgccaca ataactaaaa ctcaattctt tagctatcga
     5941 ttttattaat tgggcattgt tttcagcagc ggacatttac acagaacaga attttagtta
     6001 aagaatactt tgccaagaaa attatccagc attgttttct tttgcagcat tttttatctt
     6061 ttgatttagg attttttcaa attctttatc aatataatac aaactttgag gaccatcgcc
     6121 aatcaatttt attctatcaa taattcgttt aaattgtact tcttcttcac gttgctcatc
     6181 aacaaaaaat cttaaaaagt caatagttgc tttgtctcct tcttcctcag ctacttttat
     6241 aatatgatag atggatttag ttactgcttg ttctcctttt aatgaatatt cacaaacttc
     6301 tttgatattt ttgaatttac tttttggttg ttttaccgca ggaacaatag catgttctcc
     6361 aacatcattt atatagtcaa aaaacttcat aaagtgtgta tgctcttcat ctgattggtc
     6421 tctataaaat ttagcacaac cttccaaacc ttctctctcg caccaagatg ccactgcaag
     6481 gtaattaaat actgcatttg cttccatttc caaatgttgg tttaaaacgg cttctacttt
     6541 tttaggaatt atcataatat tgaattttat tttaatagat taggatacaa aactaactat
     6601 tactataaga aattgcaaat ttttattttt cagacctgaa aattggtaaa taatgtaact
     6661 tgtattattg ttaatatttg ataatataac aaatgttata taaaactatt tatatctcaa
     6721 ataaatactt agattttgaa ttaaaaagta atattaagtt tcatttttta ttgattttaa
     6781 ttattcaaca tcaaaccaaa gtttggtttg gtcttctgga taataattgt aattaatagt
     6841 gatttcagta ttggctggaa ttttttttcg agcaattact ttgaagatat tatcatcata
     6901 ataggcttca tacctagcat ttggactata gtcatgatta tacattgcac cgtaacctag
     6961 aacaatacca gcactttcgc catcttcacc ccaaataaaa taataatgat aaagcgttgt
     7021 tttgttgagg tgtttggtat cttttggcgg acaaacaatt actggacaaa tttcaatttc
     7081 atcaccttct tccaaatctt cagtacagaa aacacctctg cctttattcc caacttcttt
     7141 aatatataat tccatagttt ttttcttaaa aatttattga ttttctaaaa tatgctccac
     7201 catttcttta gaaccaacaa aaacgggcgt tctttgatgt aattctgttg ggataatttc
     7261 caaaattctt cttactccat ctgtaccttt tcctccagct tgttcttgaa caaaacttaa
     7321 tggattgcat tcatacatta atctcaattt tccatttttg tctttatgag tagcaggata
     7381 catataaata ccgcctttaa ttaaatttct atgaaaatca gccaccatag aaccaatata
     7441 ccttagagaa tatagttttt tattaccatc tgaagacata cagtaatcaa tatattttct
     7501 aatattttct gaaaatgaat gataatttgc ttgatttaca gaataggtta aagcatgttt
     7561 tttggtttta atatttggat gtgataaaca aaattcaccg attgaaggct ctaatgtaaa
     7621 accattcaca ccatcgccag ttgtataaac taacatagtt gatgagccat aaataacata
     7681 acctgcggca gcttgttttt cgcctgcttg taaaaagtct tctaaagtac agttttctcc
     7741 aagtttagta actcttttaa agactgaaaa tatagtacca acagtcgcat tgacatcaat
     7801 attactagaa ccatctaatg ggtcaaaaag tactacatat ttagcttttt ttctgttttc
     7861 aaaaacaaca atttcgtctt cctcttcaga agcaatacct gcacattcgc cactattttg
     7921 tagcgttttt gtaagaatag tatttgcaaa ttcatctagt tttttgacct cttcgccttg
     7981 aatattagtt ttttcagcat ctcctaaaat attatccacc aaacctgctt ttcttaaatc
     8041 tcgattgaca atcttagcag ctaagcctat atctcttaat aatcctgata attcgcctgt
     8101 ggcatcttca attttatttt gctgttcaat tataaattca tctaacgtaa ctaatcggtc
     8161 tttgattgtc ataaatttaa tttttgccaa atataataaa aaacgttttt tttactaaat
     8221 ttgcaccgct aaataaacaa aatatgagag tttttaaatt tggaggtgct tctgtaagag
     8281 atgccgcagg cataaaaaat gttgcatcaa ttattcagaa atatggtgat aagaatactt
     8341 tagtggtagt ttctgctgtt ggaaaaacaa ctaatgcttt agaagaagtt gttgcagctt
     8401 attttgacgc atctaaaggt ggaaagaaaa cacttgaacc agtaaagaac aaccatttgg
     8461 aaatttgtgc aggcttattt aatacagata atccagtata tgcacattta gaaaaagtat
     8521 tttctgagat tgaacaaaga ttcgatacaa caccatcaga taattataat catatttatg
     8581 accaaattgt ttctcaagga gagttacttt catcaattat cctttctgca tatttaaaag
     8641 aagctggaat tgataataat tggttagatg ttcgtgagat tattaaaacg gataatacat
     8701 atcgtgaagc agttgtggat tgggtaaaaa caggagagtt ggtggctaca aaagttgttc
     8761 ctatggtaaa tggaagtatt gtggttacac aaggattttt aggaagtact tcagatagtc
     8821 aaacaacaac tttaggtcgt gaaggttcag actatacggc agcaattttt gcaaatcttt
     8881 tgaatgctga aaatcaaaca atatggaagg atgttccagg cgtaatgaat ggtgatccta
     8941 gaattttttc agatgctgtt ttcttagaac aaatttccta taatgaagct gttgaaatga
     9001 cattttatgg agctactgta attcatccaa aaacaattaa gcctttacaa aataaaaata
     9061 ttccactgca cgttcgttct tttataaacc cagaaggcgt tggtacaatt gttggaaaag
     9121 aagatcaaaa aagtttgccg ccaataagaa ttgttaagaa aaaccaagca ttattaacct
     9181 taactactaa agatttttca tttattcaag aagatgtttt ggcttcaata tttagagcat
     9241 tctctaaagt gaatttaaaa ttgaatatga tgcaacaaga agctatttct tttgaagctg
     9301 ttgtggataa tcaagaagat aagattgcaa gattaatgaa atttttacaa gataatttcc
     9361 aaattgaaat tcaagaggat ttaagcattt taacgctcag acattataat gatgcgacaa
     9421 tagtacaaaa ttctgctgga aaagaagtct tattagttca aaaaacgtca acaacttatc
     9481 aatctttgat tagataattt ggtatattaa aaatgattaa aggcatatta attaatattg
     9541 atatgccttt tttattgtaa aaatgattaa ctttgcaatc cgattttgta ttaagttatt
     9601 aaattggaag atttaattta ttttttaacc atttaaaaag tcaggatttg acaacagaaa
     9661 aagaaaatcc aaatttggaa aacgacgccg aattggtagc aagaaacaaa gaagtcgtag
     9721 aagttgaaga ccttaattta gaggaaatca aaaaagaagt agttcagaaa gaatttgatt
     9781 ggaacatcaa caaaagaggt gctaatcgtt attctaaaga agaagctgct cagttagagc
     9841 aattgtacga caacacattt aatcaaattg aaaacaacga aattattaca gcaacagtta
     9901 gtgcagttac agactctgat gttgtattaa atgtaggttt caaaagtgat ggtttagttc
     9961 caagaagtga attcaaggat atgccagaca ttaaagttgg agatgaggtt ttggtatttg
    10021 ttgaaaacaa agaaaacaaa aaaggaaatc ttatcctttc acgtaaaaaa gctaaattag
    10081 ttgatgcttg ggaaagaatt aagaaagcta aagacactga tgagattgtt aaaggaaaag
    10141 taatcagcaa aactaaaggt ggtttaatcg ttgatttatt tggtatggaa acattcttac
    10201 ctggctctca aattgatatt aaaccaatta cagattatga tcaatttgta ggtaagatga
    10261 tggagtttaa agttgtgaaa atcaatgaag caattaaaaa tgcagtagtt tctcacaaag
    10321 ctttaattga atctgatatt gaatctcaaa gattagaaat tatcggtaaa ttagaaaaag
    10381 gtcaagtatt agaaggaact atcaaaaata ttactgattt cggtgcattc attgatttag
    10441 gtggtattga tggtttattg tatattactg atatttcttg gggtagaatt aatcatccaa
    10501 gtgaagtgtt acaaatggat caaaaaatcc aagtggtggt tattgagtat gatgaaaaca
    10561 aaaaacgtat ttcattgggc ttaaaacaat tacaagctca tccttgggat aatttaccag
    10621 aaggaattgt tgaaggaagc aaagtaaaag gtaaaattgt aaatattgaa gattatggtg
    10681 ctttcttaga aatcgtacca ggtgttgaag gtttaattca cgtatctgaa atatcttggg
    10741 gtaaccaacc aattaattct agagatttct tcaaattagg agacgagcat gaagctttgg
    10801 ttgttacaat tgacaaagat gaaagaaaaa tgtctgtgag tttaaaacag atggaagaag
    10861 acccttggaa aaacatcact actaaatatg cagttggtac aagacataaa ggtattgtaa
    10921 aaaatatcac tccttatggt gtatttgttg agttagatga tatcgttggt ggtatgattc
    10981 atattagtga tttatcttgg actaaacgtt ataatcatcc aaatgaatat acttcagttg
    11041 gtagtgaatt agatgtaact attttagaaa tcgattctga taatagaaaa atcgttttag
    11101 gtcataaaca attggaagaa gatccttggg atacttttga aaatcttttc ccagtaggtt
    11161 ctgtacacga agctacagta ttgaaaagag aggataaagg tgctatcgtt caattacctt
    11221 atggtatcga agcatttgct ccaactcgtc atatcaaaaa agaagatggt aagttagcag
    11281 atgtagatga ggttttaaaa ttcaaagttt tagagtttga aagagatgac aaacgtatta
    11341 ttgtttctca tactcgcgtt atcgaagatg aaaaattaga agaaaagaga aaagtagatg
    11401 gagataagcg taaagaaact agaaaacaaa aagatgcatt gaaagatgtt aatagcaaag
    11461 tagaaaaatc tacattaggc gatttaggtg cattagctga tttgaaagca aaaatggaag
    11521 gcggagaaaa ataataaata ttcaccttaa taataaacgg ctacccaaag gtagccgttt
    11581 ttatttctct ctatttcttt atctatagtg gttttaagag aatttgtaaa ctgtttttgt
    11641 atttgccaaa tatagtaatg aagctgttgc ttgtcaaatt aaaatgtttt cattgtgtct
    11701 ttcctaaaat tctgagaaaa actaatatac aacaagtcta taacaataaa agtttgtctt
    11761 atttctaaaa gtatatattg ttttttttac tttttatttt gattgaaatt gctagaatct
    11821 aatttcaaaa atgtaatttt gcaagtatgg aaatttcgtt gaattggtta agagattatg
    11881 ttgatattcc actttctgtg gaagaaatta gtgtattatt gacagatatt ggtttggaag
    11941 ttgaaggcgt tgaagattat gtatctatca aaggtggatt agaaggttta gtcgtaggat
    12001 ttgttcaaga aaaagaaaaa catccagacg cagataaatt gtctgtttgt aaagtagata
    12061 ttggaaatga agttttgcaa attgtttgtg gagcgccaaa tgttgcagca gggcaaaaag
    12121 taattgttgc cttggttggc acacaaattt ttcccacgaa aggagagcct tttttgatta
    12181 aaaaagcaaa gattagaggt gtggaatctg ttggaatgat ttgtgcggaa gatgaaatcg
    12241 gaataggaga gagccacgat ggaattttgg ttttagatga aaatactgaa attggtattc
    12301 cagtaagctc actttttaat gtttataaag ataccattat tgaaattggc ttaacaccta
    12361 atcgctcaga tgcacattct catatcggcg ttgcacgaga tttgatggcg gcgtataatt
    12421 atagaaataa caaaaatatt aagttaaaag ccattgaaac ctatcaagat actcataaga
    12481 tagaacaggg ttttaaagta tcagtagaag ataaagaaga ttgtccaaaa tatttaggta
    12541 ttctattggg aaatatttcc attcaagaat caccaatttg gttgcaaaat aagttgagag
    12601 caattggaca aaagccgatt aattatatcg ttgatgttac gaattttgtc atgcacgaac
    12661 ttggacagcc tttacacgct tttgatctta cagcaatttc aggcaatgaa attattgtca
    12721 aaaaagttaa ggaaaatacc ccatttatta ctttagatgg ttcagaaatt aaactttcag
    12781 gtactgagtt gatgatttgt aatgctgaag aaccgatgtg tatagcagga gtttatggag
    12841 ggaaaaaatc tggggtaact gaaaatacaa agtctatatt cttagaaagt gcttatttta
    12901 atcccaagtc tattagaaaa acttcaacaa aacaccaatt aaaaacagat gctgctacgc
    12961 attttgaaaa aggaatagat ccaaacatta cacaaatagc tttagaaaga gctatatttt
    13021 tattacagca aatagattca aatattactt tactttcaaa tatgtataat attgaaaatg
    13081 aggaatttaa tcattttgaa ataaagttaa attttaataa ggttaggcaa ataaccggag
    13141 cttctattac taacgaacag attctgaaaa ttttagattt attagaaatt aaggtaataa
    13201 atctaacaga ccttggtacc gatttattag tgccacctta tcgtgcagat gtacaaagag
    13261 aagttgatgt cattgaagat attttgagga tttatggatt taacaatata ttgataccat
    13321 caaaaataaa tgcttcagta aatattactg aaacaaaaaa tccagaacaa atttatgaac
    13381 aagtagccaa tgatttaact tcgattggtt atgctgagat tttaactaac ccactgtcta
    13441 aatctaaatt gattgaaaaa tttgaaccag caagggaaaa ttgggtgaat ttattgagtt
    13501 ctataaatgt agaattagat gtattgcgaa ataatatgtt attttctact ttggaaacag
    13561 taagttataa taacaatcac aaaaacagta atttaagatt gtttgagtta ggtagaactt
    13621 atcataaaaa cggacaagat tatattgaac aagagcattt aattttgtgt cattttggtt
    13681 tgaaacaaga acccaattgg aaagctactg ctcaaaaaac agatttcttt accttgaaga
    13741 cttctgttaa tcgcttatta caaaaaattg gtataaatca atttgaaata acattttcgg
    13801 aatctaagta ttttgattat ggattggaat atgcaattaa tgatgctatt attgtgaaat
    13861 ttggaaaagt ttcaagcaat tggactactt tatttgatat taaacaagaa gtttattttg
    13921 cagaatttaa ttggttgaaa ttaattgaat tatacaatag taataaagta agctataagc
    13981 caatttctaa atttccttca gttaaaagag atttggcttt attgttgaat aaagaaatta
    14041 agtttgaaga attaaaaact gttgcagaaa aaataggtaa aaaattatta caaaaaattg
    14101 atatctttga tatttataca gatgataaaa tgaaagctaa tgaacaatct tatgcattaa
    14161 gttttatatt taaagacgaa cataaaactt tacaagatgc agatattgat tccattatgc
    14221 aaaagatgat acaagtattt caaaaagaat ttaatgcaac agtaagataa tgagtaagtt
    14281 agatgataga tggaatagaa tcgtaaaaaa aattcatctt attatagaaa aaaataagaa
    14341 attagaagaa gttgaattaa ctttacgaaa taaaatcaat gaattagaga aaagtaataa
    14401 atctttaact gaacaaaaca gtgaattaaa taaagaagaa cagcaaactg aaattattaa
    14461 ccaacaaaat tatattaagg aattagaaca acaacaagag atattaaata aaaatttaga
    14521 acaaattaaa attgaaaatc aaactttaaa agaagcttta aaagttaaag aatcaaatgc
    14581 agtgatagcg aatgtagagg aaagagaagc attaaagaaa caaattgaac aacatatcaa
    14641 taaaattgat gatattttag aattgataaa cccataatgg cagaacaaaa taatatcaat
    14701 atcatggtgg ctggtcgccc atacaaactc aaagtttctg gagaagaaga gagttttgta
    14761 agacaggctg cgaaagagat taacgataaa atttctgaat tccagaaaac gttattaact
    14821 cgtgataagc aagatttctt gtcgatgata gcactacaaa cgactgctga tatgcttcaa
    14881 tctaaaaata aaattaccaa ttctgcttca ttagaagcca aatttgatga attggaacaa
    14941 ctattagata ggagtttagg aattacccta ttttgatttg tatagatttg ctttaagttt
    15001 ttattgtctt ttattattgt agatttattt ataaacaata aaaaaggagg aaaaatgata
    15061 gaaatagtta caggtgtagt tgcattgatt attggaattg taataggcaa atttttgttt
    15121 aaagcaaatg tagataagca attacaagaa gctaaattac ttgaggaaaa agctcaaagt
    15181 atggtagata atgccaaaaa agaatcagaa attattgtta aagaagctag tactttggcc
    15241 gaagcaacca agaaagataa aatgttggag gcaaaagaac atttctttaa gttgaaagaa
    15301 gaacacaata aggaaattga ccaaagaaat aaaaaaacgg tcgagactga aaatagattg
    15361 aagcaagtag aacaatcatt aaaagatcgt ttggcaaacg ctggcaaaca agaaaaggat
    15421 aatgaagaaa taaaagccaa acttcaactt caattggaag tgatgaatgc taaacaagca
    15481 gatttagaaa aggcacatca agaacatatt agcaaattag agcaaatcgc aaaactttca
    15541 gccgaagatg ccaaagctca actggtagaa cagttaaaag gtaaggcaca aactgaagct
    15601 attgctcacg ttaaaatggt gatggatgaa gctaaattaa aagcaaataa agaagccaaa
    15661 aaaatcatca tacaatctat acaaagagtg gcagcagaaa atgctatcga aaatgccatt
    15721 tctgttttta atttacaaag cgatgaccaa aaaggtcaaa ttattggtcg tgaaggtaga
    15781 aatatcagag ccttagaagc tgcaacaggt gtcgaaatca ttgttgatga tacacctgaa
    15841 gcgattgtaa tctcaggttt tgacccttat aaaagagaga ttgcaagact atcattacaa
    15901 cgcttagtta ctgatggtag aattcaccca gctagaattg aagaagttgt ggcaaaaacc
    15961 gagaagatgt tggagcaaca aattatggag ataggagagc gtacggttat tgatttaggc
    16021 attcatggtt tacatcctga attgataaag atagttggaa aaatgcgttt ccgttcttct
    16081 tatggacaaa acttattaca acattcacgc gaggtttcta atttatgtgc tattatggct
    16141 agtgaattag gcttaaatgc aaaaatggca aaacgtgctg gtttattaca cgatatagga
    16201 aaagtttctg atgaagatcc agaattatct cacgctttat taggtgcaaa attatgtgag
    16261 cgatatggag agcatcctgc tattgtgaat gcagttggtg cacaccatga tgagatggaa
    16321 atgttatttg tgatttctcc aattatccaa gcttgtgatg ctatttctgg agctagacct
    16381 ggagcaagac gtgaaataat ggaaagctat atgaagcgta ttgcagaatt agaaaaattg
    16441 gctttaaatt ataatggtgt tgaaaaagcg tatgcaattc aagcaggtcg tgaattacgt
    16501 gttattgttg aagctggaaa aacttcagat aaagatgtag aagaaacagc atttaaaatt
    16561 gcacaacaga tccaagatga aatgacttac cctggtcaaa taaaagttac ggttattcga
    16621 gaaactagag gtgtgtctgt tgcaaaataa ataatagaga ctataatgtg aaagaggctg
    16681 tccgaaaagg gcagcttttt tttttagaca atatctctaa aactgtgtaa acctattaca
    16741 gaaaaagaaa atttctatcc ataaatctta tcaataaatc aatcataata ggagtggagc
    16801 atatcggagt cgaaccgatg acctcttgac tgccagtcaa gcactctagc cagctgagct
    16861 aatgccccaa tacataggat tgataaagat ataaattagc atttaaacaa ccaattttca
    16921 atgatttttt ttccgttggg tgtcattaca ctttcaggat gaaa
//
LOCUS       DAOQCW010000049        16364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1264_length_16364_cov_5.574223, whole genome shotgun sequence.
ACCESSION   DAOQCW010000049 DAOQCW010000000
VERSION     DAOQCW010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16364
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1264_length_16364_cov_5.574223"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1677
                     /gene="uvrC"
                     /locus_tag="PLS16_05850"
     CDS             <1..1677
                     /gene="uvrC"
                     /locus_tag="PLS16_05850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNJ60637.1"
                     /translation="AKNLKKRVSSYFTKDDHPAKTKLLVKQIQRIEYTIVNTEKDALL
                     LENSLIKQLQPKFNINLKDDKTYPYIKIFNEQFPRVIFTRNKIDDGSEYFGPYTSVFN
                     VRYIYNLLKEMYPIRTCSYNLSKENIRKKKFKVCLEYHLGNCLGPCEALQTEEDYNRN
                     IEQIRNILKGKTRVVKDILREEIEIYAQQLAFEKAADLKKKLDALNEYIENSIVSNLE
                     TEDAHIFGYVENETKAFINYLYIYDGNIVQTKSFFIQKNIEEDKEDILTMAIVEQIGL
                     LKNKALVLLPFDISFSDESYQFFVPKIGEKKKLVELAQKNAFVQMQKTIAHTKTPKSE
                     RILELMKSELKLKSLPVHIECFDNSNFHGDYPVSACVVFKNAQPSKKDYRHFHVKTVV
                     GPNDFDTMKEAVFRRYHRLLEEEADLPQLVVIDGGKGQLSVAMESLKKLNLENEIQLI
                     SIAKRLEEIYYPNDSIPLHLNVKSETLKVLQHIRDEAHRFGITFHRHIRDKGTLQTEL
                     TKIEGVGELSAQKLLAEIGSVEQIKSASLNQLEKVVGKRIAKKVYDYYLQ"
     gene            complement(1822..2148)
                     /locus_tag="PLS16_05855"
     CDS             complement(1822..2148)
                     /locus_tag="PLS16_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HNJ60638.1"
                     /translation="MITVSDIAKNRIEHLWVENKLSADHFVRISVSSGGCSGLSYNMN
                     FDDDFKAGDEAFEDKGIKLVVDAKSLLYLLGTELDFSEGLSGKGFYFNNPNASRTCSC
                     GESFSL"
     gene            complement(2197..2682)
                     /gene="purE"
                     /locus_tag="PLS16_05860"
     CDS             complement(2197..2682)
                     /gene="purE"
                     /locus_tag="PLS16_05860"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNJ60639.1"
                     /translation="MAKIAIVMGSDSDSPTMSEATKILDTLGVAYDYTIVSAHRTPHK
                     MYEFAENAHKNGVKVIIAGAGGAAHLPGMIAAISPLPVIGVPVQSKTLSGWDSLLSIV
                     QMPGGVPVATVAIGGAKNAGILAAQILATEDTTLLDRIIAYKKQLNDEVEQKCQDFKP
                     F"
     gene            complement(2686..3258)
                     /locus_tag="PLS16_05865"
     CDS             complement(2686..3258)
                     /locus_tag="PLS16_05865"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNJ60640.1"
                     /translation="MINLILFGPPGSGKGTQAVKLAEHFNLLHISTGDLFRNEMKNET
                     PLGLEAKSYISKGELVPDEVTIGMLSNKVDENIGKVDGFIFDGFPRTEPQAEALDKLL
                     TLKNTEITKVLALDVPEEEIVGRILERAKTSGRADDADETIIRNRFNIYLNETAQVAD
                     HYKKVDKFVALDGVGTIESIFEALKAEIEK"
     gene            complement(3398..5110)
                     /locus_tag="PLS16_05870"
     CDS             complement(3398..5110)
                     /locus_tag="PLS16_05870"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNJ60641.1"
                     /translation="MTIQAVKRPDRQYFPNDLVFENWQSVQEFYASLQASTVEHLDSL
                     MEFIAKRSELDKIVEEAYRWFYVKMTCDTENEVYKNAYESFITDTMPELMKVSNELNL
                     KVYQSPAFNQLDENRFFIYKRGLKSAIELYREENIPLFQEEQLKAQEFGSISGQMTIE
                     HDGKDITLSQAAVFLQNEDRNLRQTVYEKIQNRRFQDKDKLQQLFSDLVQIRHKIALN
                     TGFENYRDYKLAELGRFDYSVKDCEAFHESIEKVVIPVVNKIYEKRKQNLNLEQLKPY
                     DLDVNENGGTPLHPYQTEQEFKDKSIACLNSVDSYFAECISIMDKMDYLDLSSRKGKA
                     PGGYNMTLPEIGIPFIFMNGAGTQRDVETMVHEAGHAVHSFLMRDLAYNFDGEITSEI
                     AELASMSMEFFTYDGLNFFYNEEDKRKAIQTHLKGVISMLPWIALIDKFQHWIYTNPH
                     HSVEERTATWNEFFSRFSSKVTDWSDYQMFKDIVWQKQLHLFEVPFYYIEYGIAQLGS
                     IGMYRNYLTDKASTVQNYKKALSLGYTKTIPTMYETAGVKFDFSTENVQSLVDFVAKK
                     IDGF"
     gene            complement(5122..5529)
                     /locus_tag="PLS16_05875"
     CDS             complement(5122..5529)
                     /locus_tag="PLS16_05875"
                     /inference="COORDINATES: protein motif:HMM:NF037329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HNJ60642.1"
                     /translation="MELKDIVAVTGVSGLKKLVSNRNDGLILSDLDDTNKKFYPSRTH
                     LFSPLDNISIYTDDDMVSVGEVLGMIKKNETTIPVISSTSSNEDLKKYMAQILPNYDR
                     DKVKVSDIKKLVRWFEIINPYNIIKTEDDKVAE"
     gene            complement(5613..6686)
                     /gene="prfA"
                     /locus_tag="PLS16_05880"
     CDS             complement(5613..6686)
                     /gene="prfA"
                     /locus_tag="PLS16_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNJ60643.1"
                     /translation="MLDKLEAIKIKYKDLEQQLSDAAVVADAQRFRKVGKEYRDLEPI
                     VDAYEKYKNLLSNITFNKQVVAEEKDEELKEMAKMELDEQLAQLPELEEEIRLLLIPK
                     DPEDDKNAILEIRAGTGGDEASIFAGDLLKMYLRYCDSKGWKTEIMNENPGTSGGFKE
                     VVVEVNGDGVYGILKYESGVHRVQRVPETESQGRVHTSAATVAVLPEAEEVDVEIKES
                     DIRRDVYRASGAGGQHVNRTESAVRLTHIPTGTVAECQDGRSQIKNYESALKVLRTRL
                     YEAAINAHNDAIAKQRKTLVSTGDRSAKIRTYNYPQGRVTDHRINLTLYNLGEIVNGD
                     LNPIIDKLIFEENAEKLKMQNMH"
     gene            6800..7306
                     /locus_tag="PLS16_05885"
     CDS             6800..7306
                     /locus_tag="PLS16_05885"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNJ60644.1"
                     /translation="MKQILLAVSLLIGISTFSQNVIVGSKAPEIEQAKPDGTMLKLSS
                     LTGNVVLIDFWASWCYPCRKQNPNVVALYNQYKGKKWIKGTKGFTVFSVSLDNNAESW
                     KKAIEADGLIWPNHVSDLKGWSSAPGAVYGIRSIPQTLLIDEGGFVIARNPSYDFIRE
                     FLNKRLKQ"
     gene            7412..8011
                     /locus_tag="PLS16_05890"
     CDS             7412..8011
                     /locus_tag="PLS16_05890"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNJ60645.1"
                     /translation="MQHEEIKSTEEQQEKVENTTENNSIPEELVSNEVVDKEIDTTNE
                     EPVSAVSELEQLQQELNEQKDKYLRLFAEFDNFKKRSAKEKRDFTLVAAQDIMKELLA
                     VIDDYKRANKSFSQDGNKENFADGLQLIFDKFNKILASKGLKEMESIGTDFDFGKHEA
                     IAEIPAPTEELKGKVIDEVEAGYLLNSVIIRYAKVVVGK"
     gene            8035..9183
                     /gene="dnaJ"
                     /locus_tag="PLS16_05895"
     CDS             8035..9183
                     /gene="dnaJ"
                     /locus_tag="PLS16_05895"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNJ60646.1"
                     /translation="MKRDYYEILGVNKTASAEEIKKAYRKLAVQYHPDKNPGDKAAEE
                     KFKEAAEAYDILSNADKKAKYDRFGHQAFGGGGGGGNYGGGMNMEDIFSQFGDIFGGG
                     GGFESFFGGGGRRTERGKGQRGSNLRVKVKLDLKEVANGANKKIKLKKYVTCDSCHGS
                     GAKDSSSYQTCGTCNGSGAVRKVTQTFLGQMATTSTCPTCHGEGKIITNKCTKCKGEG
                     RMYDEETISIDVPAGVSDGIQLSMSGKGNAGIRGGYAGDLLIGIEEVPHPDLKRIENN
                     VIYNLYLNIADVALGTQVEVPTIDGQARIKIPKGTQSGKLFRLQGKGIPSLNGYGKGD
                     QIVEVKVFTPVDLTSEETALLEKLRSSRNFNPDAQKEKNKGFFDKLFN"
     gene            complement(9190..9939)
                     /locus_tag="PLS16_05900"
     CDS             complement(9190..9939)
                     /locus_tag="PLS16_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HNJ60647.1"
                     /translation="MFRYKGRGRTFTHNLSIAILLSFVAGIVNVAGYLSVHQLTTNVT
                     GHFAFLIEEVNQSNFTKAIFLFLYILSFLFGSFTSNFLVELNLKRNSNYVYLLPIAIE
                     CSILFGAALFGQYIIYKLPNLLAFLLLFAMGLQNALVTVISKSIVRTTHLTGLFTDLG
                     IELSQLFFYKESSQQSRLLKSIRLRMAIISSFFIGGIVGGLTYSQIQLYALIIAGIVL
                     LFGVVINYLKYRYLKLKRRYQSKKIPSNNDN"
     gene            complement(9973..10761)
                     /locus_tag="PLS16_05905"
     CDS             complement(9973..10761)
                     /locus_tag="PLS16_05905"
                     /inference="COORDINATES: protein motif:HMM:NF017672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006672 - ceramide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ceramidase domain-containing protein"
                     /protein_id="HNJ60648.1"
                     /translation="MKINTFTSTVLVFIISSFTIGLMIVYGGFGVTDGTGAVFCEASR
                     TGNLILQPVNSWSNIGFVTAGLYASWQITYGNIRNNNIFYKSSFFAKFICGLMVLLGP
                     CSMAMHATETSLGGLFDMNSMYLIAGFMVGYSFVRFYNLDIKYFFLIFFICVIIGNAS
                     PQLHKIINIDFFLGNLAFGLMCFLGLLHEFLYHIKKIVHAEIKYAVLCGLTFIIAFII
                     WNFGRDGSCICFPYSPFQLHGVWHILCALSVYFLFRYYISENKI"
     gene            complement(10758..11513)
                     /locus_tag="PLS16_05910"
     CDS             complement(10758..11513)
                     /locus_tag="PLS16_05910"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNJ60649.1"
                     /translation="MTIKKHIPNSLTLLNLFCGCCGIVFCLYNQYQYVPYLIFTALLA
                     DFLDGFVARALKVKSDLGAQLDSLADMTTFGVLPSVMMLMLLQLLEKNNQLAFEINSS
                     NDTTQVIFSYIALLSFIYAICACLRLAKFNIDTRQTVNFIGVPTPAGAMFILGIYANY
                     FSQNALFNFIYQQWFLIISTALISLLMISELSLFSLKGNPFNYKENKLRFVFLILCIP
                     QIFIFKWLGLSTIILSYILFSIIDFYTTKKQTS"
     gene            complement(11555..12025)
                     /locus_tag="PLS16_05915"
     CDS             complement(11555..12025)
                     /locus_tag="PLS16_05915"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNJ60650.1"
                     /translation="MEDIKFMQRAIELSEMGMQTGSGGPFGCVVVKNGKIVGEGFNQV
                     TSTNDPTAHAEVVAIRNACKNLNTFQLEGCEIYTSCEPCPMCLGAIYWARPEKIYYAN
                     SREDAKAIGFDDSFIYDEIPLAMHQRKIPMINLNNQEAKLVFNKWQNKVDKTEY"
     gene            12138..12722
                     /gene="gmk"
                     /locus_tag="PLS16_05920"
     CDS             12138..12722
                     /gene="gmk"
                     /locus_tag="PLS16_05920"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNJ60651.1"
                     /translation="MEKAIILSGPSGVGKNTLGDFLLARFPQLAYSVSATTRKIRTGE
                     KEGKDYYYISVPEFENKIQNNELLEWQEVYEETYYGTLKSELDRIHQHQQVPLLVIDV
                     YGAINVMKNIEIETLTIFIDIVSLEELEKRLTQRGTETAEKVAKRMEKAVVEVQEKSA
                     FDVVIVNNDLITAQNELATVVAQFIGAEYSDLKL"
     gene            12751..13785
                     /gene="proB"
                     /locus_tag="PLS16_05925"
     CDS             12751..13785
                     /gene="proB"
                     /locus_tag="PLS16_05925"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNJ60652.1"
                     /translation="MKKELLVYKIGTAGITNKQGLIDIKVLQEIVRQLALLHKNYNIL
                     IVSSGAVGTGKKFIKDYSGKIEERKAAAAIGNPILIQKYANAFAKYDIAIAQSLCERE
                     HFSNRKKFLQLRSTYEELWQNNIIPIANENDVVSSYELKFSDNDELATLLAIGLNASN
                     LLIATSVDGVLDAENNVVSTITKFDQSILSLARKDKTNVGLGGMLSKLTFAKLASSLG
                     IQVNIFSAKKENSILDALENKIGTFIQAKVVAQSARQKWLASGSNVIGSVEIDNGAKS
                     ALLKRKSLLAVGVRNILHHFGNGEAFEIVDDDGHVVAVAKSKIGSKQLTDTSSKQNII
                     LAHADEIVLL"
     gene            complement(13812..15059)
                     /locus_tag="PLS16_05930"
     CDS             complement(13812..15059)
                     /locus_tag="PLS16_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase family
                     protein"
                     /protein_id="HNJ60653.1"
                     /translation="MKPQNILAQNFKLPNGTVIKNRLFKSAMSEGQGHKNGAPKESLI
                     NLYKVWAEGGLGICVTGNVMIDKRYLGEPGNVIIENEDNLAQLKAWAEAATLNNTACW
                     VQLNHPGKQSPRGINKENVAPSAIPFRTDMRAVFATPRALTIEEIKDIIKRFGTAAAI
                     VKKAGFSGVQIHGAHGYLVSQFLSPHHNVRNDEYGGNAENRRRFVVEVYKAMRAKVGK
                     DFPIGIKLNSADFQKGGFTEKESMETIKTLSDLGIDLIEISGGTYESPEMMVVNKMKE
                     STKKREAYFMDFATKVRKQINTPLVVTGGFRSLNGMANAISSKDVDFVGIARSLAIEP
                     DLPNRLLDGQEPQYIIQPRTTGIRMIDSSGILEIKWYADQMKRIGNGEKPIPNENPYQ
                     TLFRLIKSYFVSGGKKPTRMRAK"
     gene            15276..16055
                     /locus_tag="PLS16_05935"
     CDS             15276..16055
                     /locus_tag="PLS16_05935"
                     /inference="COORDINATES: protein motif:HMM:NF018054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60654.1"
                     /translation="MNYWVELSIEYASQKSYLDDLFQVYPTIPEGIREINQERWTNIE
                     KAFEQKNNDLLIRELLKLELFPIKDSYIAYLKRDGSAIARNPKTINRISGRLYEMGLD
                     KIFERCSEPKETNRQIGPLFRRWLNSKALGIEPVSYEEFISSNDDAILNGSDAQLMKF
                     ANEFLKYEHPKGLDFVGRFNGKLVIGEAKFLTDFGGHQNAQFNDAISTIMTDINAVKI
                     AILDGVLYIKGNNKMYKDITGKYKDLNIMSSLVLREFLYQL"
     gene            16068..>16364
                     /locus_tag="PLS16_05940"
     CDS             16068..>16364
                     /locus_tag="PLS16_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60655.1"
                     /translation="MQLDYPNKKTEQQIFDSIPNIFLEQINNSDSSNLLIQANNLVAL
                     KQLITQHNLAGKIDLIYIDPPFATNNTFTITDGRASTISNSKNGNVAYSDTLKGF"
BASE COUNT         5750 a         2552 c         2663 g         5399 t
ORIGIN      
        1 gcaaaaaatt taaagaaaag agtgagttct tactttacaa aagatgacca tcctgcgaaa
       61 actaagttgc ttgtaaagca aattcaaaga attgaatata ctattgtcaa tacggaaaaa
      121 gatgcacttt tattagaaaa ttcactcatc aaacaattac aacctaaatt caatatcaat
      181 ttaaaagatg ataaaactta tccatatatc aagatattta atgaacaatt tccaagagtg
      241 atttttacta gaaataaaat agatgatgga agtgaatatt ttggccctta tacttctgtt
      301 tttaatgtta gatatattta taacttattg aaagagatgt atccaattcg cacttgttct
      361 tataatttat ctaaagaaaa tattaggaaa aagaaattta aagtttgttt agaatatcat
      421 ttaggtaatt gtttgggacc atgtgaagcc ctgcaaactg aagaagatta caatagaaat
      481 attgaacaaa ttagaaatat tttgaaaggt aaaactcgag ttgtaaaaga tattttgcga
      541 gaggaaatag aaatttatgc tcaacaatta gcatttgaaa aagctgctga tttaaagaaa
      601 aaacttgatg cattgaatga gtatattgaa aattcaatcg ttagtaattt agaaaccgaa
      661 gatgctcata tttttggcta tgtagaaaat gaaactaagg catttataaa ttatttgtac
      721 atctatgatg gtaatatagt tcaaacaaaa tcttttttta ttcaaaaaaa cattgaagaa
      781 gataaagagg atattttaac catggcaatt gttgaacaaa taggtttgtt gaaaaacaaa
      841 gctctagtat tattgccttt tgatatatct tttagtgatg aaagttatca gttttttgtt
      901 ccgaaaatag gagaaaagaa gaagttggtc gagttggcac aaaaaaacgc ttttgtacaa
      961 atgcaaaaaa caattgctca tactaaaaca ccaaaaagtg aacgtatttt ggaattgatg
     1021 aaatctgaac taaaattaaa atcattgcct gttcatattg aatgttttga taattctaat
     1081 tttcatggag attatcctgt ttctgcttgt gtagtcttta aaaatgctca acctagcaag
     1141 aaagattata gacactttca tgttaaaaca gttgttggtc caaatgattt tgatactatg
     1201 aaagaagctg tttttcgtag ataccatcgt ttgttagagg aagaagccga tttgccacag
     1261 ttggttgtaa ttgatggcgg aaaaggacag ttgagtgttg caatggaaag tttgaagaag
     1321 ttgaatttag aaaatgaaat acaattgatt tccattgcca aacgtttaga agaaatttat
     1381 tatccaaatg atagcattcc tttgcatttg aatgttaaat ctgaaacttt gaaagtatta
     1441 caacatatac gtgatgaggc acacagattt ggaattacct ttcatagaca tattagagat
     1501 aaaggcactt tacaaaccga actgacaaaa attgaaggtg taggagaact gtctgcacag
     1561 aaattattag cggaaattgg ttcagtagag cagataaaat cagcatcttt aaatcaatta
     1621 gaaaaagtgg ttgggaagcg tattgcaaaa aaggtttatg attattatct tcaataaatt
     1681 ctctgccctc aaaaggtgtt atcacctttt gttgcaattc tctattcaac actaaataat
     1741 tttttttgtg tgttggcagg agataatacc tgccaaaggc gaagcgataa attcgtgatt
     1801 tttattttga aattaaaaaa tctacaaaga aaaactttcc ccacaagagc aagttctgga
     1861 ggcattggga ttgttaaagt aaaacccttt gccactcaat ccttccgaga aatctaattc
     1921 tgttcctaaa agatataata agctttttgc atcaacaact aatttaatgc ctttgtcttc
     1981 aaaagcttca tcgccggctt taaaatcatc atcaaagttc atattgtacg ataagccaga
     2041 acaaccgcca ctactgacag aaatacgaac aaaatgatcg gcagacagtt tgttttccac
     2101 ccaaagatgt tcaattctat tttttgcaat atcacttaca gttatcatac ttaaaactta
     2161 aaggtaataa ttaagacaaa ttaaaagata ataagtttaa aatggtttga agtcttgaca
     2221 tttttgctct acttcatcat ttaattgttt cttataagca ataattctat ctaaaagagt
     2281 cgtgtcttca gtggctaata tttgtgcagc caaaatacca gcattctttg caccgccaat
     2341 ggctacagtc gcaactggaa cgccaccagg catttgaaca atagatagca aactgtccca
     2401 gccacttaaa gtcttggatt gtacaggcac accaattaca ggtaatggtg aaatagcagc
     2461 aatcattcct ggaagatgag cagccccacc tgcaccagcg ataattactt ttacaccatt
     2521 tttatgtgca ttttcagcaa attcatacat tttgtgaggc gttctgtgtg cagatacaat
     2581 cgtatagtca taggcaacac ccaatgtatc taatattttt gtagcttcac tcatagttgg
     2641 tgaatcgctg tcagaaccca ttacaattgc aattttagcc atagttcatt tttctatttc
     2701 agcttttaaa gcttcaaaaa tagattcaat tgtgcctacg ccgtctaaag caacaaattt
     2761 atctactttt ttatagtggt cagctacttg agcagtttcg ttcaaatata tattaaatct
     2821 atttcggatg atagtttcat ctgcatcatc agctctacca gaagtttttg ctctttctaa
     2881 aattcttcca acaatttctt cttcaggaac atctaatgct aaaaccttag taatttctgt
     2941 attttttaaa gttagtaatt tatcaagagc ttcagcttga ggttcagttc ttggaaaacc
     3001 atcgaaaata aaaccatcta ctttgccaat attttcatca actttatttg ataacatacc
     3061 aattgtaact tcatcaggaa cgagttctcc tttggaaatg tagctttttg cttctaatcc
     3121 aagaggtgtt tcatttttca tttcatttct gaataagtct ccagtagaaa tgtgtaataa
     3181 gttaaagtgt tcagctaatt ttacagcttg agttcctttt ccacttccgg gaggtccaaa
     3241 taatattaaa tttatcatgt cgttgtaaat caattaagtt acaaaaataa aggtatttat
     3301 gtataaatgt gttgatttat gattaagaat ttgaagaata ggcgtttgga aagtattaat
     3361 tctgtattat tcattgaata aagtaaaaat gaagacctca aaagccatca atctttttag
     3421 ctacaaagtc aaccaaagat tgaacatttt cagtagagaa atcaaatttc acacctgctg
     3481 tttcatacat ggtcggaatg gtttttgtat agcctaaact taatgctttt ttgtaattct
     3541 gcacagttga ggctttgtcg gttagatagt ttctatacat tccaatggag cctaattgtg
     3601 cgatgccata ttcaatataa tagaatggaa cttcaaataa atgtaattgt ttttgccata
     3661 caatatcttt aaacatttga taatcgctcc agtctgttac cttacttgaa aatctactaa
     3721 aaaattcatt ccaagtagcg gtacgctctt caacactatg atgtgggttg gtataaatcc
     3781 aatgttggaa tttgtcaatc aaagcaatcc aaggcaacat agaaattacg ccttttaaat
     3841 gggtttgtat ggcttttctt ttatcttctt cattgtagaa aaaatttagc ccatcatacg
     3901 taaaaaattc catactcatt gaggctaatt cagctatctc agaggtaatt tctccatcaa
     3961 aattataagc taaatctcgc attaagaaag agtgaacagc atgtccagct tcgtgaacca
     4021 ttgtttccac atctcgttga gtaccagctc cattcataaa aataaatggt atgccaattt
     4081 ctggtaaggt catattataa ccacctggag cttttccttt tctgctactc aaatctaaat
     4141 aatccatctt atccattatg ctgatgcatt cagcaaaata actatccacc gaatttaaac
     4201 aagcaataga tttgtcttta aattcttgct ctgtttgata aggatggagc ggcgttccac
     4261 cgttttcatt cacatctaaa tcataaggtt tgagttgttc tagatttaga ttttgtttac
     4321 gcttttcata aattttattg acaacaggaa taacaacttt ctctatagat tcatgaaagg
     4381 cttcacagtc ttttacacta taatcaaatc gacctaattc ggctaattta taatctctgt
     4441 aattttcaaa tccagtattt aaagcaattt tatgtcgaat ttgtactaaa tcagaaaata
     4501 gttgttgcaa tttatcttta tcttgaaaac gtctattttg gattttttca tataccgttt
     4561 gacgcaaatt tctgtcttca ttttgtaaaa aaactgctgc ttgactaagt gtaatatctt
     4621 ttccatcatg ctcaatcgtc atttgccctg aaatactgcc aaactcttgt gctttcaatt
     4681 gctcttcttg aaatagcgga atattttctt cacgatataa ttctatggca ctttttaagc
     4741 ctcttttgta aatgaaaaat ctattttcat ctaattgatt gaatgctgga gattgatata
     4801 cttttaggtt cagttcatta gaaaccttca ttaattcagg cattgtatct gtaatgaaag
     4861 attcataagc gtttttgtaa acttcgtttt ctgtatcaca ggtcatcttc acataaaacc
     4921 aacgataagc ctcttcaaca attttgtcta attcacttct tttagcaata aattccatta
     4981 aagaatctaa atgctcaact gttgaggctt gcaaacttgc atagaattcc tgtaccgatt
     5041 gccaattttc aaatactaaa tcatttggaa agtattgtct atccggtctt tttactgctt
     5101 gaattgtcat ttttttattt tttattctgc tactttatca tcttctgttt tgatgatatt
     5161 gtatggatta atgatttcaa accatcgcac caattttttt atatcgctta cttttacttt
     5221 atctctatcg taattaggta aaatttgagc catatatttt tttaaatctt cattagaaga
     5281 agtactgctg ataacaggaa ttgttgtttc atttttcttt atcataccca aaacctcacc
     5341 aacagaaacc atatcatcat ctgtatatat agaaatatta tctaaaggag aaaataaatg
     5401 cgttcttgac ggataaaatt tcttatttgt gtcgtccaaa tcagataata ttaaaccatc
     5461 atttctattc gatactaatt ttttcaatcc agatacacca gttacagcaa cgatatcttt
     5521 taattccata aatgtaattt tttgcgtaaa aatagtaaaa taatagcaat tgcaattcta
     5581 aaataatctt atcaataaaa tcacaataga ttttaatgca tattctgcat ctttaatttc
     5641 tcagcatttt cctcaaaaat taatttatcg atgataggat tcaaatcgcc gtttacaatt
     5701 tctcctaaat tgtataatgt caaattaatt cggtggtcag ttacacgacc ttgaggataa
     5761 ttataagttc taatttttgc acttctgtct ccagtagata ccaacgtttt tctttgttta
     5821 gcaattgcat cgttgtgtgc attgattgct gcttcataca atcttgtacg taaaactttt
     5881 aaagcacttt cgtagttttt aatttgtgag cgaccatctt gacattctgc tacagttcct
     5941 gttggaatat gcgttaaacg tactgccgat tccgttctat taacgtgctg tccaccagca
     6001 ccacttgctc tataaacatc tcttctaata tcactttctt taatttcaac atctacttct
     6061 tctgcttctg gtaaaacggc aactgttgca gccgaagtat gtactcttcc ttgcgattct
     6121 gtttcaggta cacgttgcac acgatgtaca ccactttcat attttaaaat gccataaacc
     6181 ccatcaccat ttacttctac cactacttct ttaaagccac ccgaagtacc tggattctca
     6241 ttcatgattt ctgttttcca gcctttgcta tcgcaatatc taagatacat ttttaataaa
     6301 tctcctgcaa aaatactagc ttcatctcct ccagttccag ctctaatttc taaaatagca
     6361 tttttgtcat cttcagggtc ctttggaatt aataataatc tgatttcttc ttccaattca
     6421 ggtaattgtg ccaattgctc gtccaattcc atttttgcca tctctttcag ttcttcatct
     6481 ttttcttctg caacgacttg tttgttaaaa gtgatattac tcaatagatt tttatatttt
     6541 tcgtaagcat caacaatagg ttctaagtct ctatattctt taccaacttt tctaaatcgt
     6601 tgggcatcag ccacaacagc agcatctgat aattgttgtt ctaaatcttt gtattttatc
     6661 tttatcgctt ctaatttgtc taacatagca gcaaaaatac aattttatta tttggcattc
     6721 agtgtagtag cacagatgca aagaaaattt aacaaaaatt ggaatgaaat ttgtctaatt
     6781 ttgatgaaat aaatttttta tgaagcaaat attacttgca gtctctttat taataggcat
     6841 tagtacattt tctcaaaatg ttattgttgg ttcaaaagct ccagaaattg aacaagcaaa
     6901 acctgatgga acgatgttaa aactgtcttc acttacagga aatgtagttt taatagattt
     6961 ttgggcatct tggtgctatc cttgtagaaa gcaaaaccca aatgtagttg cattatacaa
     7021 tcaatataaa ggtaaaaaat ggataaaagg aacgaaaggt tttactgttt ttagtgtttc
     7081 tttagataat aatgctgaat cttggaaaaa agctattgaa gcagatggtt tgatttggcc
     7141 aaatcacgtg agtgatttaa agggttggag ttctgcacct ggagcagttt atggtattcg
     7201 ttctattcct caaacattat taatagatga aggtggtttt gtaattgcca gaaatccttc
     7261 ttatgatttt attcgtgaat ttttgaacaa aagactaaaa caatagattg ttttttgctt
     7321 tagaagtaaa tgagattttc ttttatgtca aaatgtccta attatctcat tggcaaactt
     7381 tttgaccaat aagatagaaa taggggaaat tatgcaacac gaagagataa aatcgactga
     7441 agaacagcag gaaaaagtag aaaatacaac tgaaaacaat tcaattcctg aagaattggt
     7501 atcgaatgaa gttgtagata aggaaataga tacaacgaat gaagagccag tttctgcggt
     7561 ttctgaatta gaacaattac aacaagaatt aaacgagcaa aaagacaagt atttacgttt
     7621 gtttgcagag tttgataatt tcaaaaagcg tagtgctaaa gaaaaacgag acttcacact
     7681 tgtagcagca caagatatta tgaaggaatt attggcagtt attgatgatt ataaaagagc
     7741 caataagtca tttagtcaag atgggaacaa agaaaacttt gctgatgggt tacaacttat
     7801 ttttgataaa tttaataaga ttttagcgtc aaaaggcttg aaagaaatgg aaagcattgg
     7861 cactgatttt gattttggaa aacacgaagc tattgcagaa attcctgctc caactgaaga
     7921 attgaaagga aaagtaattg atgaagtgga ggctggttat ttattaaata gtgtcatcat
     7981 tcgttatgca aaagtggttg taggcaaata acataaaaca gaacaatcta ttaaatgaaa
     8041 agagattact acgaaatatt aggcgttaat aaaacggcaa gtgccgaaga aattaaaaaa
     8101 gcatatcgaa aattagcggt tcaatatcac ccagataaga atcctggaga caaagctgct
     8161 gaagagaagt ttaaggaagc cgcagaagct tatgatattc taagcaatgc tgataaaaaa
     8221 gcaaaatacg accgttttgg acaccaagca tttggtggtg gcggcggtgg cggaaattat
     8281 ggcggtggca tgaatatgga agatattttc tctcaatttg gagatatatt tggtggcggt
     8341 ggcggctttg agtcgttttt tggtggcggt ggaagaagaa ccgaaagagg taagggacaa
     8401 agaggtagta atttgagagt aaaagtgaag ttagatttga aagaagttgc gaatggtgca
     8461 aataaaaaaa tcaaacttaa aaagtatgtt acttgcgatt cttgtcatgg aagtggtgcc
     8521 aaagatagta gttcatacca aacttgtgga acttgtaatg gaagtggtgc tgtaagaaaa
     8581 gttacccaaa ccttcttagg acaaatggca acaacatcaa cttgtcctac ttgtcatggc
     8641 gaaggtaaaa taattacaaa taagtgtaca aaatgtaaag gtgaaggtag aatgtatgac
     8701 gaagaaacca ttagcattga tgtgcctgct ggtgtaagcg atggtatcca attgtcgatg
     8761 agtggaaaag gaaatgctgg aattcgtggt ggatatgctg gagatttatt gattggaata
     8821 gaagaagtgc cacaccctga tttgaaacgc attgagaata atgttattta taatctatat
     8881 ttgaatattg cagatgtagc tttaggtact caagttgaag tgccaacaat tgatggacaa
     8941 gcaagaataa aaattccaaa aggtactcaa agtgggaaat tattcagatt acaaggcaaa
     9001 ggtattccaa gtttgaatgg ttatggaaaa ggcgatcaaa ttgtagaagt caaagttttt
     9061 acaccagttg atttaacctc tgaagaaaca gcattgttgg aaaaattaag aagttcaaga
     9121 aacttcaatc ctgatgctca aaaagagaaa aataaaggtt tttttgataa attattcaac
     9181 taaatatttt tagttatcat tattagacgg tatcttttta gattgatacc gtctttttaa
     9241 tttaaggtat ctatacttca aataattgat aacaacacca aaaagtaata caattcctgc
     9301 gataataagt gcatataatt gtatttgtga ataggttaag cctccaacaa ttccacctat
     9361 aaaaaaggag gaaataatag ccattctaag tctaattgat tttagtaatc tggattgttg
     9421 tgatgattct ttataaaaga aaagttgtga tagctcaata cccaaatctg tgaatagacc
     9481 tgttaaatgt gtggttcgaa ctatagattt tgagattact gtaactaaag cgttttgtaa
     9541 tcccattgca aataataata aaaatgctaa taaattaggt agtttgtaga taatatattg
     9601 tccaaacaaa gcagctccaa aaaggataga acattcaata gctattggta aaagatacac
     9661 ataattacta ttgcgtttta aatttagttc tactaaaaaa tttgaagtaa acgaaccgaa
     9721 tagaaacgat aagatataca aaaatagaaa aatagcttta gtgaagttgg attgattaac
     9781 ttcttcaatt aaaaaggcaa aatgtcctgt aacattagtg gtcaattgat gaacggataa
     9841 ataaccagcg acattaacaa tacctgccac aaatgataat aatatggcaa tgcttaaatt
     9901 gtgtgtgaat gttctgcctc tacctttata ccgaaacatt tgaagttttg ttattcatta
     9961 acgatatcaa aattaaattt tattttctga aatataatat cgaaaaagga aatatacgga
    10021 taaggcacac aagatatgcc aaacaccgtg caactggaaa ggagagtatg gaaagcaaat
    10081 acaactgcca tctctgccaa aattccaaat aataaatgca atgataaaag ttaagccaca
    10141 aaggactgca tacttaattt ctgcatgtac aatttttttg atgtggtata aaaattcgtg
    10201 caataagcct aaaaagcaca tcagtccgaa ggcgagatta cctaaaaaga aatcgatatt
    10261 aataatctta tgtagctgag gagaggcatt cccaattatc acacaaataa aaaaaataag
    10321 gaagaaatac tttatgtcta aattatagaa gcgaacaaaa gaataaccta ccataaatcc
    10381 tgcaataaga tacatactgt tcatatcaaa aagcccaccc aatgaggttt ctgttgcatg
    10441 cattgccata gagcaaggac ctaataaaac cattaagcca cagataaatt tggcaaaaaa
    10501 gctacttttg taaaaaatat tattgtttct aatattgcca taagttattt gccaagaagc
    10561 atataaacca gcagttacaa atccaatatt tgaccaagaa tttactggtt gtaaaattaa
    10621 atttccagtt cttgatgctt cacaaaatac agcacctgtt ccatcagtta ctccaaagcc
    10681 accatataca atcatcaagc ctatggtaaa agaagaaatg ataaaaacta agacggtaga
    10741 ggtaaaagta tttattttca agatgtttgt ttttttgtag tataaaaatc tataatacta
    10801 aatagaatat aagataaaat tatcgtgctt aatcccaacc atttgaaaat aaaaatttga
    10861 ggaatacaga gtattagaaa tacaaatcgc aatttatttt ctttatagtt gaatggattc
    10921 ccttttaaac taaataggga taattcacta atcattaaca atgaaataag tgcagtagaa
    10981 ataattaaaa accattgctg ataaataaaa ttgaataaag cattttgaga aaaataatta
    11041 gcataaatac ctaaaataaa cattgcacct gctggtgttg gaacaccaat aaaattaacc
    11101 gtttggcgag tatcaatatt aaatttagcc aatcgtaaac aagcacatat tgcatatata
    11161 aaactaagta aagcgatata agaaaatatt acttgagttg tatcattaga gctatttatt
    11221 tcaaaagcta attgattatt tttctctaaa agttgtaata gcatcaacat catcacgctt
    11281 ggtaaaaccc caaaagtggt catatctgcc aaagaatcta attgtgcacc taaatctgat
    11341 ttcactttta aagcacgtgc cacaaatcca tctaggaagt cagctagaag tgcagtaaaa
    11401 ataagataag gaacatattg atattggtta taaaggcaaa atacaatacc acagcaacca
    11461 caaaataaat taagtaaagt aagagaatta ggaatgtgtt ttttgattgt cattggttca
    11521 aaattaagaa aattattgaa ttgtaaggag ttatttaata ttctgtttta tcaaccttat
    11581 tttgccattt attaaaaact aatttggctt cttggttatt caaatttatc ataggtattt
    11641 tacgttgatg cattgccaaa ggaatttcat catatataaa tgagtcatca aagccgatgg
    11701 ctttagcatc ttcacgtgag ttggcgtagt aaattttttc aggtctagcc caataaatag
    11761 cacctaaaca catcggacat ggttcgcaag aagtataaat ttcacaacct tctaattgaa
    11821 aagtatttaa gtttttacaa gcatttcgta tggcaacgac ctctgcatgt gcagttgggt
    11881 catttgttga agtaacttga ttaaatcctt cgcctacaat ttttccattt ttgacaacca
    11941 cacatccaaa aggaccacca ctgcccgttt gcattcccat ttcagacaat tcaatcgctc
    12001 tttgcataaa ttttatatct tccataaatc aattgttatt tgtaaatatt tatccaaaga
    12061 tgaacattat ttaagttaga aacaacaagg taacattaaa aatagctaca taaaatattc
    12121 tttaattatc ttcgcacatg gaaaaagcaa taatactttc tggtccatca ggtgtaggta
    12181 aaaatacatt aggtgatttt ttgctggcaa gatttccaca attggcttat tccgtttcgg
    12241 caacaacaag aaagattaga actggcgaaa aagaaggcaa agattattac tatatctcag
    12301 tgccagaatt tgaaaataag attcaaaata atgaattgct ggaatggcaa gaagtttatg
    12361 aagaaactta ttatggcact ttaaaatcag agttggatag aatacatcaa caccaacaag
    12421 ttccactttt ggtaatagat gtttatggtg ctatcaatgt gatgaaaaat attgagatag
    12481 aaaccctaac aatttttata gatattgtaa gtttagaaga attagaaaaa agattgacac
    12541 aacgaggaac agaaactgct gaaaaagtag cgaaacgaat ggagaaagcc gttgtggaag
    12601 tacaagaaaa atctgcattt gatgtcgtta ttgtaaacaa tgatttaatt actgcccaaa
    12661 atgaattggc tacagttgtt gctcaattta taggtgctga atactcggat ttaaaattgt
    12721 aaatttgcat ttcggattaa ttattcatag gtgaaaaaag aacttttagt atataaaatc
    12781 ggaactgctg gaattaccaa caaacaaggt cttattgata ttaaggtgtt gcaagaaatt
    12841 gttagacaat tagcattgtt gcacaaaaat tataatattc taattgtatc atctggtgct
    12901 gttggaactg gaaaaaaatt cattaaagat tattctggaa aaattgaaga acgaaaagca
    12961 gcggctgcaa ttggtaatcc aattttaatt caaaaatatg caaatgcgtt tgcaaaatat
    13021 gacatcgcaa ttgcacagag tttgtgtgaa agagaacatt tttccaatag aaaaaaattt
    13081 ctacaacttc gttctactta cgaagaatta tggcaaaata acatcattcc tattgccaat
    13141 gaaaatgatg tggtaagttc gtatgaatta aaattttctg ataatgatga gttggcaacc
    13201 ttattggcaa ttggtttaaa tgcatctaat ttattgattg caacaagtgt tgatggtgtt
    13261 ttagatgcag agaataatgt tgtttcaaca attacgaaat ttgaccaatc tatactttca
    13321 ttagctcgaa aagacaagac aaatgttggt ttggggggaa tgcttagtaa actgacattt
    13381 gcaaaattgg caagttcatt aggcatacaa gtcaatattt ttagtgcaaa aaaggaaaat
    13441 tctattcttg atgctttaga aaataaaata gggactttta ttcaagcaaa agttgttgcc
    13501 caatcagcaa gacaaaaatg gttggcaagt ggtagtaatg tgattggttc tgttgaaatt
    13561 gacaatggtg cgaaaagtgc tttattgaaa agaaaaagtt tattagcagt cggtgttcgg
    13621 aatattttac atcattttgg aaatggtgaa gcatttgaga ttgttgatga tgatggacat
    13681 gttgttgcgg tggctaaatc aaaaattggg tcaaaacaat taacagacac aagcagtaaa
    13741 caaaatatta tattggctca cgcagatgaa attgtattgc tataatttac caaaataata
    13801 gcctcaaata attacttagc tcgcattcta gtaggtttct ttccacctga tacaaagtaa
    13861 cttttgatta atctaaataa tgtttgatat ggattttcat ttgggatagg tttttctcca
    13921 ttcccaattc ttttcatttg atctgcatac cattttattt ctaaaatacc agaactatca
    13981 atcattctga taccagttgt gcgtggttgg ataatatatt gcggttcttg accatccaat
    14041 aaacgattgg gtaaatcagg ttcaatagcc aaagaacgtg caatgcccac aaaatcaaca
    14101 tctttagaag atattgcatt tgccatacca ttcaaactgc gaaagcctcc agtaacaact
    14161 aagggagtat tgatttgctt tcttactttt gtagcaaagt ccataaaata agcctctcgt
    14221 tttttagtac tttctttcat tttgttgacc accatcattt caggtgattc ataagtacca
    14281 ccagaaattt ctattaaatc aatacctaaa tcggacaatg tttttatggt ttccatagat
    14341 tctttctcag taaaaccacc tttttggaaa tctgctgaat ttaatttaat accaatcgga
    14401 aaatccttgc ctactttcgc tctcattgct ttatatacct ctactacaaa acgtcttcta
    14461 ttttcggcat ttccaccata ttcatcattt cttacattat gatgtggaga taaaaattga
    14521 cttactaaat agccatgagc tccatgaatt tgaacacctg aaaaaccagc tttttttaca
    14581 atggcagcag cagttccaaa tcgttttata atatctttta tttcttctat cgttaatgca
    14641 cgtggagttg caaaaacagc acgcatatct gttctaaaag gaattgctga tggtgctaca
    14701 ttctctttat ttatacctct tggcgattgt tttcctgggt ggttgagttg cacccaacaa
    14761 gctgtattat ttaatgttgc agcttccgcc caagctttca attgtgctaa attatcttca
    14821 ttttcaataa ttacattacc cggttcgcct aaatatcgct tgtctatcat tacattgcca
    14881 gtaacacaaa tacctagccc accttctgcc caaactttat ataagttaat gagtgattct
    14941 tttggagcac catttttatg accttgacct tcactcattg ctgatttaaa aagtctattt
    15001 ttaataacag ttccattagg taatttaaaa ttttgtgcta aaatattttg aggtttcata
    15061 tactttaata ttggttacag ataattcttg tgcaatataa tatttattga ttgaagaata
    15121 gtacttcaag aaacttatta ttaaaggtta ttttgcagaa ttaaaatgct gatttttttt
    15181 atatctataa gaatgttgtg aactaagtta aaacctgccg aacaaatatt gaccctaaac
    15241 ataatctgca aattattttt tgtaaatttg acaatatgaa ctattgggta gaattaagta
    15301 tagaatatgc aagtcagaaa tcctatttgg acgatttgtt tcaagtatat ccgactatac
    15361 ccgaaggaat tagagaaatt aatcaagaac gttggacaaa tattgaaaaa gcatttgagc
    15421 aaaaaaacaa tgacttactt attagagaac ttttaaaact agaacttttt cctattaaag
    15481 acagttacat tgcttacctc aaacgagacg ggagcgccat tgcccgaaat cctaaaacta
    15541 taaacagaat tagtggacga ttgtatgaaa tggggctgga caaaattttt gaacgttgta
    15601 gcgagcctaa agaaacaaat agacaaattg gacctttgtt cagacgttgg cttaactcaa
    15661 aagcattggg aattgaacca gtttcttacg aagaatttat atcaagtaat gatgacgcta
    15721 ttttgaatgg aagcgatgct caactgatga aatttgcaaa cgaatttcta aaatacgaac
    15781 atccaaaagg gttagatttc gttggaaggt ttaacggaaa acttgtcatt ggagaagcca
    15841 agtttttaac tgattttgga gggcaccaaa atgcacaatt caatgatgca atcagtacga
    15901 taatgacaga catcaatgcc gttaaaatcg caattcttga tggagttttg tatatcaaag
    15961 gaaacaacaa aatgtacaaa gacattacag gaaaatacaa agatttgaat attatgagca
    16021 gtttagtatt gcgtgaattt ctttatcaac tttaaaattt tgaaattgtg caattagatt
    16081 accctaataa aaaaactgaa cagcaaattt ttgatagtat tccaaatatt tttttggagc
    16141 aaatcaataa ttcagatagc tccaatttgc taatacaagc caataattta gtggcattaa
    16201 aacaacttat aacacaacat aatttagcag gaaaaattga tttaatttat attgacccac
    16261 cgtttgcaac aaacaataca tttaccatta cagatggaag agcaagtact atcagtaatt
    16321 ctaaaaatgg aaatgttgct tattcagata ctttaaaagg tttt
//
LOCUS       DAOQCW010000050        16321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1267_length_16321_cov_4.873417, whole genome shotgun sequence.
ACCESSION   DAOQCW010000050 DAOQCW010000000
VERSION     DAOQCW010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16321
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1267_length_16321_cov_4.873417"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..629)
                     /locus_tag="PLS16_05945"
     CDS             complement(21..629)
                     /locus_tag="PLS16_05945"
                     /inference="COORDINATES: protein motif:HMM:NF036723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60656.1"
                     /translation="MREQHTPYSNFYQAIPKLENEYTNDEFLQDYLATNLPKDILKSI
                     QPDLEQFGQRVATELVEYANACENNPPKLISFNAYGDRVDEIKVAPEWQKLEDIAAEE
                     GLVAIGYERKFGIYSRLYQFVKLYLYTPSSAIYTCPLAMTDGAARLIEFYGDDFLKDK
                     PFKGIISRNPKDFLTSGQWMTERTGGSDVARSMTIAHKADDG"
     gene            755..1192
                     /locus_tag="PLS16_05950"
     CDS             755..1192
                     /locus_tag="PLS16_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNJ60657.1"
                     /translation="MLSKKTKYAINALVHLAKEPPKQPVLISDISEKQHIPKKFLEAI
                     LLDMKNAGLVNSKKGKGGGYYLLKEPADINLAEVIRLFDGAIGMLPCVTHKYYEKCDE
                     CEDEVTCGIRHTFLSIRNETVELLKQSTLAQIVETETALKTHD"
     gene            1185..1844
                     /locus_tag="PLS16_05955"
     CDS             1185..1844
                     /locus_tag="PLS16_05955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HNJ60658.1"
                     /translation="MISKDSLLFLEQLSKNNNKEWFDINRKIYQNYRTEFIEFIQQWI
                     NELSHFDTEIENIEAKNCIFRINRDIRFSKNKQPYKTNFGAYISKGGKKSNYGGYYLH
                     LEPNNCFFGAGIYETEPLELAKIRQEIDYNFKEFKTIVENQKFQKTFGTLNIENKLKK
                     APKGYDENNEAIEYLKLKNFIIGTRIEDKMICSPNFNQYLVELSKQSIPFIRFLNQAL
                     D"
     gene            complement(1846..2388)
                     /locus_tag="PLS16_05960"
     CDS             complement(1846..2388)
                     /locus_tag="PLS16_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60659.1"
                     /translation="MFFSFFSCKKAEQKLIDDGFITYTIPQGGHYSDNSKLVMLSAIS
                     ELKFTVVFDGSAKYISALPENQLDINKLYGFSDNNTQHHENSARIGWRWNEDELQLFA
                     YIYNNSVRSFQYIKSVSLNDEINCSIKIEANKYVFTVDGTTIEMPRFSTSPNGYKLYP
                     YFGGDEVAPHKILIRIKEIK"
     gene            2534..3682
                     /locus_tag="PLS16_05965"
     CDS             2534..3682
                     /locus_tag="PLS16_05965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNJ60660.1"
                     /translation="MLRILILNYEYPPIGGGAGIVTKHLAEEFIKLGHQITILTTWFE
                     GETENYKHNNFEIIRLKSKRKSTFQSNPLEMYDWIKKSKQYFNQNDIRGKFDVCFANF
                     TLPGGDVALFLKQKFNLPYIILSHGHDIPWAFPKQMFLWHTLSYFWIKKICTQSAYNI
                     ILSDELKTMADNLVPSNKSKNIILNNGLKIHDFNKQFSGHILNIIFIGRLVEQKNPLL
                     FLESIRQLRDLNIPFRVEIYGDGELRKDMELYVQKNKIEQIYFKGKVNHNDVINALKN
                     TDLLVSSSKSEGMALAILEALTFGVYVVVTNVSGNDKLILENINGNIVKEQRASIIAT
                     KIADFYHQKFTKNYTYPTDYMEQLQAQFSWQGIAKQYIDLLSKAANKN"
     gene            complement(3679..5898)
                     /gene="purL"
                     /locus_tag="PLS16_05970"
     CDS             complement(3679..5898)
                     /gene="purL"
                     /locus_tag="PLS16_05970"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HNJ60661.1"
                     /translation="MSVIVNEELAKTLGLLPEEFIKIQEILGRVPNYTELSIYSVMWS
                     EHCSYKNSIKYLKKLPRKGKALLVEAGEENAGLVDIGNGWACAFKIESHNHPSAVEPF
                     QGAATGVGGISRDIFTMGARPIASLNSLRFGLIDNKRTQHLLKGVVKGIGHYGNCFGV
                     PTVAGEVYFDDCYQVNPLVNAMSVGIVKIGDTISATSYGKGNPVYIVGSATGKDGIHG
                     ATFASEDLGEDAEDKIPSVQVGDPFVEKLLLEATLEVIKTGAVIGMQDMGAAGITCST
                     SEMSAKGEHGMKIWLDKVPTRQKNMLPFEILLSESQERMLIVVEKGKEALVEAVFDKW
                     DLNCAVIGEVTEGNTLEYYMNGELVAEVPADTLVLGGGAPVYDRAFSKPKYHEERDKY
                     SIEQISEPNIDNLKEISKAVVAYPNIASKRFIYKQYDSMVGVNNTGINEPSDANIIRL
                     KESNKGLVVTVDCNSRYVHADAEVGGAIAVAEAARNITCSGGKPVAITNCLNFGNPYD
                     PEVYWNFVHALQGMSASCIKFDTPVTGGNVSFYNQSPNRAVNPTPTIGMLGVIEDVQN
                     KMTLDFKNESDLIYQIGASKNDISSSEYLFNYHKVELSPAPYFNLDEEYAMQNAVSDL
                     IENKLVQSVHDISEGGLFIAIMESAMPRNYGFEINTSPSIRKDAFLFGEAQGRAIASV
                     TAEQKAAFELLLTQKQIPFELVGQVKNKAVVVDGLDFGNIEEYKDLYDTSIEKYMEA"
     gene            6005..7294
                     /locus_tag="PLS16_05975"
     CDS             6005..7294
                     /locus_tag="PLS16_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60662.1"
                     /translation="MLLEILYPLLFILLLSVIISKSKYFKSTEIGSPILVLGFLLKCI
                     SAFYFGYLFNKNILTGNDTFNYLRNSEIIYSSIQESWLTYFKLTFGRNWHSNPDLNLI
                     PYIDNMDFWYDNSNYLMVRINAIIRLFSFGIYNVHAVIFAFISFIGIYNLYLFFENKV
                     FNKRILQIILFGIPSIVFWTSGVHKEAIVIFALGMSLYNTDKLFSTQYERKHVILTIL
                     GLFILGSIRIYILAFLIPLYIAIILSQKISFPKRTFRFYAALTIFLCIISLALIDTLI
                     PNFSLFNEFIVRRSHFLRISGNTTFDVVTNFNSIEGFIMVIIDAIINPFIHPFPNESN
                     LFLVFIASLETYLMLFIILATIFSLSYKNLYKNPYALFCILFSLSILFLIGLIVNNSG
                     AIVRYRSIAIPFLLIGLCLLYKPKESLQLETEVGITK"
     gene            complement(7306..7905)
                     /locus_tag="PLS16_05980"
     CDS             complement(7306..7905)
                     /locus_tag="PLS16_05980"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNJ60663.1"
                     /translation="MFWVRTPNIIQKSFPKVIWEMNEEARALIKMPSCTFMGKKIYLT
                     FDDGPHPIITPWVLEQLKANNVKATFFCLGEHIEKYPEIFEQIKKDGHTIGNHGYKHY
                     NGWRISRKNYLENYQKGKAISGSNLFRPPYAKVRAMDKFTKGEIVICSVLPHDYHPKV
                     TPEKCYENIQNHLRENDILVLHDNEKAWTKLQYILPKCL"
     gene            complement(7911..8999)
                     /locus_tag="PLS16_05985"
     CDS             complement(7911..8999)
                     /locus_tag="PLS16_05985"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNJ60664.1"
                     /translation="MHKFWIIIWLCLVNSLNIYANSFSINDFYKENKRLDYVTDSVFN
                     SLNNKQKAAQIIMYAVSSTDNVYTYNNSKKLLEDTIISNVIFLKGYLSNIANQIKDYN
                     AIQTSFNNIYACDCEPSLFNMKYFGSPNVLKTSQLNSDSLVLDAAKTISSVLDKIGIQ
                     INLSPLADNAFNAAVINNRSFGNNIDDIIHRAAQFIDESKTFNIATTIKHFPGHGNVI
                     GDSHKNLVYINGPLKEVPTFYHLIQKSEPVFTMMGHIAIKDNEQFNTAGLPSSIDKNI
                     VSNLLKNDLGFRGIVITDAMNMLGVSTIPNADFKALEAGNDIVLMPKNPRLLSEQIVE
                     ELDKQTALAYQIEDSVKKMIRLKICLTK"
     gene            complement(9031..10290)
                     /locus_tag="PLS16_05990"
     CDS             complement(9031..10290)
                     /locus_tag="PLS16_05990"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNJ60665.1"
                     /translation="MAYKEPIQIGGYTKAGFEKVRDVFQFNFYHRKEVGASCCVYFEG
                     EKVVDLYAGYKDKDRRKHWEEDTLALLFSTTKGISSLAMAILHSRGLLDYDEKVATYW
                     AEFAVNRKENITVRQLFAHQAGVFSNDKSFSYELLKDEKALSSALAKQKPYWKVGDRQ
                     GYHAWTIAMYQNELMKRIDPKGRNLSEFVYEEIAHPLGVEMYIGFPKEINIERKADNI
                     PFNPLEAITGGDNRDFGKLIKNITNPFSMFYKSLLNPPFAINLNNFNKRKYIEIQNGA
                     ALGFSNARGLATIYNEFATGGKKLGIRKETLEELAKAPIAPKIRRTDLVLGVNIPFSV
                     GFAKPSTYQNFGIDYKAFGTFGAGGSGAFADPTMKVAFAYVMNKMGTHIANDPRELAL
                     RQATYDCVLAIEHNRNLKKEIFASKVE"
     gene            complement(10392..10940)
                     /locus_tag="PLS16_05995"
     CDS             complement(10392..10940)
                     /locus_tag="PLS16_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNJ60666.1"
                     /translation="MNWNEYLIKFEQILNDENPTAPYNDPEYFEYTKLNHSRLKRWLK
                     TAVLTEETKAVIHAIKEKQNWILITEPWCGDAAHITPIIYMMSILNENIHLELQLRDT
                     DSEIDNYLTNGGKSIPIFIVRDADGKDKFRWGPRPVNAQNIFNEQKANGEPFEKIKEA
                     LQHFYNKDKTASIQHELTALLK"
     gene            complement(10979..11692)
                     /locus_tag="PLS16_06000"
     CDS             complement(10979..11692)
                     /locus_tag="PLS16_06000"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNJ60667.1"
                     /translation="MFLKNKIAILTGASSGLGVAIAKALVKSDVLVYGLARNKVALSN
                     LQESLGYLFHPIEMDITNREALLNWIQITFNEKHLPDILVNNAGIGNFGKVDEIPTEK
                     WLEMVNTNLNGMYFITSLIVPFLKMNKQVSHIINIGSILATMGREDGTAYCATKYGVR
                     GFSEALLKELRPFDIKVTCVHPGSIETDFFKNSGIEKHSNMLQPDAIADTIIHILKTP
                     DNMLIDEIIMRPLHAQKPL"
     gene            complement(11702..12151)
                     /locus_tag="PLS16_06005"
     CDS             complement(11702..12151)
                     /locus_tag="PLS16_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNJ60668.1"
                     /translation="MKSRLDYGQVAPKAIKGLLEIENYIHHSNLEKSLFELVKTRASQ
                     INGCGYCLDMHTKDARKEGETEQRLYALSAWRETPFFTERERIALEWTEALTLIAGNP
                     ISDELYERVSKEFNEQDLIALTMTIIAINGWNRLAIPFRTVAGSYNP"
     gene            complement(12154..14931)
                     /gene="acnA"
                     /locus_tag="PLS16_06010"
     CDS             complement(12154..14931)
                     /gene="acnA"
                     /locus_tag="PLS16_06010"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HNJ60669.1"
                     /translation="MSTKSPKFQKLIQVNGVDYNYCSLKDFPKGNIEQLPFSIRILLE
                     NVLRNYDGFSVTDEHIQTLANWTPEPKDKDIPFMPSRVLMQDFTGVPAVVDIASLRSE
                     FVRHGKDGQKINPAIPVDLVIDHSVQVDYFGTNYAYEKNVEVEYERNKERYELLKWAQ
                     NGMNNFTVVPPGMGICHQVNLEYLAKGITNRDGWLFPDTLVGTDSHTPMVNGIGVVAW
                     GVGGIEAEAAMLGQPIFFTCPEVVGLKLTGKIPNECTATDMVLSITRILRDKGVVGKF
                     VEVFGEGLNNLSVTDRATIGNMSPEFGCTVTYFPIDDRTLEYMRNTNRSEEQINIVEK
                     YCKENLLWRTGNEQIKYSSVVEFDLNSLEPTVSGPKRPQDKIFVKDLKNKFADLLQQE
                     YNRTYTQVNERKEAAWLAEGGSGTEFLFGKVPHDGHTSNAEIVREDLHTVRIKNKNQE
                     YNFSDGSIAIAAITSCTNTSNPAVMIGAGLLARNAIERGLRTKSWVKTSLAPGSKVVT
                     QYLQRSGLNYDLDALRFHIVGYGCTSCIGNSGPLPPHIAEAIEKGELVVASVLSGNRN
                     FEARVHPQTKMNFLMSPMLVVAYALVGRVDVDLMHDALGFDPNGEPVYLKDIWPSRVE
                     IQNTINDCVKQEDFKAVYDVIFEGSEDWKDLKVKLDQNFEWRNDSTYIKEAPFFENIS
                     NQLSPITDIKSARVLLYLGDSVTTDHISPAGSFKEDTAAGQYLIANGVEKVAFNSYGS
                     RRGNHEVMMRGTFANVRIKNKIIDKEGGFSKYFPTGEVKTVFETAMAYQKDKTPLIVL
                     AGKEYGSGSSRDWAAKGTVLLGIKAVIAESFERIHRSNLVGMGVAPLVFMDGENAQSL
                     GLDGSEVFDIIGFENDLVPHKILEVKAVHPSGKEIDFKVKARLDADIEIEYYKNGGIL
                     QYVLRDYLKKD"
     gene            complement(15253..>16321)
                     /locus_tag="PLS16_06015"
     CDS             complement(15253..>16321)
                     /locus_tag="PLS16_06015"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNJ60670.1"
                     /translation="LNKYKFEIGQHRDKNVIWILFPNDATLRNELKQHFPSVQFSGSK
                     KCWYLNDVKAIREQLGLHEKTSFEKVDFELITEVNRPALQRMHETLLLKGYSQNTIKS
                     YCGEFMQLLVTLRNIDVNTLTSERLRSYMLYCTKKLKLSENTLNSRMNAIKFYFEQVM
                     KYEKLFFQEIPRPKQKTSLPKVLSKNEILRLFGQVDNLKHQIILKLCYGMGLRVSEIV
                     NLKISNIDSGRMLVHIENSKGKKDRYVPLPETILETLRDYFREYRPKEFLFEGQYGGQ
                     YSIRSVQAIFKTAMKKAKIKKTIGIHGLRHSYATHLLEAGTDMVFIQKLLGHKDVRTT
                     QIYAKVTNKQLAKVKSPLDDL"
BASE COUNT         5442 a         2872 c         2199 g         5808 t
ORIGIN      
        1 catttatgtc ctttaagttc ctagccatca tcggctttat gagcaatcgt catagaacga
       61 gcaacatcag agccaccagt tcgttcggtc atccattgtc cactagttaa aaaatctttt
      121 ggatttctag aaataattcc tttaaatggt ttatctttta gaaaatcatc accataaaat
      181 tcaatcaatc ttgcagcacc atctgtcatg gcaagtggac aagtataaat tgctgatgat
      241 ggtgtatata aatagagttt tacaaattga tacaatcttg aataaatacc aaattttcgt
      301 tcataaccaa ttgcgactaa accttcttcg gctgcaatat cttctaattt ttgccattca
      361 ggtgctactt taatttcatc tactctatcg ccatacgcat taaaggatat tagtttaggc
      421 ggattatttt cacaagcatt tgcatactcc actaattctg tagcaacacg ctgtccaaat
      481 tgttccaaat ctggttgtat agattttagt atatctttcg gaagattagt tgctaaataa
      541 tcttgtaaaa attcatcgtt tgtatattca ttttccaatt ttggtattgc ttgataaaaa
      601 ttggagtatg gtgtatgttg ctctctcatg ctataatatt tgtaaaatgt aattctaagg
      661 taactagatt ttcaaacaaa aacaaacaga taagctctaa attatattta ggattttata
      721 agatatttta ttattttagc taaaactttt atcgatgttg tcaaagaaga caaaatatgc
      781 cataaatgcc ctagtacact tagccaaaga accaccaaaa caacctgtct taattagtga
      841 tatttcagag aaacagcaca ttcctaaaaa gtttttagaa gccattttat tagatatgaa
      901 aaatgctggc ttagttaatt ctaagaaagg aaaaggcggc ggttactatc ttttaaaaga
      961 acctgctgat attaacttag cagaagtcat tcgtttattt gatggtgcaa ttggaatgtt
     1021 accttgtgtt acacacaaat attatgaaaa atgtgatgag tgtgaagatg aagtaacttg
     1081 tggaattcgc catacttttt tatccataag aaatgaaact gtagagttgc taaaacaaag
     1141 tacattagca caaattgttg aaacagaaac tgcattaaaa acacatgatt agcaaagata
     1201 gcttactttt tttggaacag ttatcaaaga ataacaataa agaatggttt gatattaatc
     1261 gaaaaatata ccaaaattac agaactgaat ttatagaatt tattcaacaa tggattaatg
     1321 aattaagtca ttttgatact gaaattgaaa atatagaagc aaagaattgt atattccgaa
     1381 tcaatagaga tattcgtttt tctaaaaaca aacaacctta taaaacaaat tttggagcat
     1441 atatttcaaa aggtggcaag aaaagtaatt atggtggcta ttacctacat cttgaaccga
     1501 ataattgctt ttttggagct ggaatatatg aaactgaacc attagaattg gcaaaaataa
     1561 gacaagaaat agattataat ttcaaggaat ttaaaaccat tgtagaaaac caaaaatttc
     1621 aaaaaacatt cggcacttta aatatcgaaa ataaactaaa aaaagctcca aaaggttatg
     1681 atgaaaacaa cgaagctatt gaatatttaa aattgaaaaa cttcattatt ggaaccagaa
     1741 tcgaagataa aatgatttgt agtccaaatt ttaatcaata tttagtggaa ttatccaaac
     1801 aatccattcc ttttattcgc tttttaaacc aagcattgga ctaaactatt ttatttcttt
     1861 gattcgtatt aatattttat gtggagcaac ttcatctcct ccgaaataag gatataattt
     1921 ataaccattg ggcgatgttg aaaatctagg catttcgata gtagtgccat ctactgtaaa
     1981 aacatattta ttagcttcta ttttaataga acaatttatt tcatcattca gagaaacaga
     2041 tttaatatat tggaaactac gaactgaatt attataaatg taagcaaata attgtaattc
     2101 atcctcattc caacgccatc caatacgagc actattttcg tgatgttgtg tattattatc
     2161 cgaaaatcca tataacttat taatatctaa ttgattttct ggaagtgcag atatatattt
     2221 tgctgaacca tcaaatacga cagtaaattt cagttctgaa atagctgaaa gcattaccaa
     2281 tttactatta tctgaatagt gtccgccttg gggaattgta taagtaataa aaccatcatc
     2341 aattaatttt tgttcagcct ttttacaaga gaaaaaagaa aaaaacacta tccacaaaag
     2401 aagtcgtaaa ttaattttca tataattcta tactgataaa gataaaataa attgaaatat
     2461 aattatataa attttaatcc tattttggaa atagtttatc cacttaaatt tattattttt
     2521 agcaaaactt aaaatgctta gaatactcat tttaaactat gaatatccac caattggtgg
     2581 tggtgcaggc attgtaacaa agcatttggc tgaagagttt ataaaactag gacatcaaat
     2641 tactattcta acaacttggt ttgaaggaga aactgaaaat tacaagcata ataactttga
     2701 aattatccgt ttaaaatcta agcgaaaaag tacttttcag tcgaatccat tagaaatgta
     2761 tgattggatt aagaaaagta aacagtattt taatcagaat gatattcgag gaaaatttga
     2821 tgtatgtttc gctaatttta cacttcctgg cggtgatgtt gctttgtttt taaaacaaaa
     2881 attcaattta ccttatatta ttttatcgca tgggcatgat attccttggg catttccaaa
     2941 gcaaatgttt ttatggcaca cactctccta cttttggata aagaaaattt gtacacaatc
     3001 agcttacaat attattcttt ctgatgaatt aaaaacaatg gctgacaatt tagtacctag
     3061 caataaatct aaaaatatca tactcaataa tggtttaaag attcatgatt ttaataaaca
     3121 atttagtgga catattttaa atattatttt cattggcaga ttagtggaac aaaaaaatcc
     3181 tctattattt ttagaaagta tccgacaatt aagagatttg aatatacctt ttcgtgtaga
     3241 aatttatggc gatggcgaat taagaaaaga tatggaatta tacgttcaaa aaaataaaat
     3301 tgaacagatt tattttaaag gaaaagtaaa tcacaatgat gttataaatg ctctaaaaaa
     3361 tactgattta ttagtatctt cctcaaaaag tgaaggaatg gctttggcta ttttagaagc
     3421 actcactttc ggtgtatatg tggtggtaac aaatgttagt ggcaacgata aattaattct
     3481 tgaaaacatc aatggcaata tcgttaaaga acaaagggca tcaataattg caaccaaaat
     3541 tgcagatttc tatcatcaaa aatttacaaa aaactataca tatccaacag attatatgga
     3601 gcaattacaa gcacaatttt cttggcaagg aattgcaaaa caatacatag atttgttatc
     3661 aaaagcggct aataaaaatt aagcttccat atatttttcg atagaagtgt cgtataaatc
     3721 tttatattct tctatatttc caaaatctaa accatcaacc acaactgctt tatttttcac
     3781 ttgtcctacc aattcaaaag gaatttgttt ttgagtaagt aatagttcaa atgctgcttt
     3841 ttgttcagct gttactgatg caattgcacg tccttgagct tcgccaaata aaaatgcatc
     3901 tttgcgaatt gaaggacttg tatttatttc aaacccataa ttccttggca ttgcactttc
     3961 cataatagca ataaataaac caccttcact aatatcatga actgattgaa ctaatttatt
     4021 ttcaattaaa tcagaaaccg cattttgcat agcgtattct tcatctaaat taaaataggg
     4081 tgctggagat aattctactt tatggtaatt gaataaatat tcagaagatg atatatcatt
     4141 tttagaggca ccaatctgat aaattaaatc actttcattt ttaaaatcta aagtcatttt
     4201 attttgcaca tcttctatca caccaagcat tccaattgta ggcgtaggat taactgctct
     4261 atttggcgat tgattataaa aagaaacatt tccaccagtt acaggtgtat caaacttgat
     4321 acaacttgca gacatacctt gtaaagcatg tacgaaattc caataaactt ctgggtcata
     4381 aggatttcca aaattcaaac agttggtaat cgcaacaggt tttcctccac tacaagtgat
     4441 atttcttgca gcttcagcaa ctgcaatagc tccgcctact tctgcatcgg catgtacata
     4501 acggctatta caatccacag taacgaccaa gcctttgtta ctttctttta aacgaataat
     4561 atttgcatca ctcggttcat taattccagt attattcact ccaaccatcg aatcgtattg
     4621 cttataaata aatctttttg aagcaatatt aggataagca actactgcct ttgaaatttc
     4681 ttttaaatta tcaatgtttg gttctgaaat ttgctcaata gaatatttat cacgttcttc
     4741 atgatattta ggtttagaaa atgctctatc atacactgga gctcctccac ctaaaaccaa
     4801 agtatcagct ggcacttcag caactaattc tccattcata taatattcca aagtatttcc
     4861 ttcggtaact tcaccaataa cagcacaatt caaatcccat ttgtcaaaaa ctgcttcgac
     4921 taatgcttct tttcctttct caacaacaat taacattcgt tcttgtgatt cagacaataa
     4981 gatttcaaaa ggcaacatat ttttttggcg tgttggaact ttatccaacc aaattttcat
     5041 accatgttca ccttttgcac tcatttcaga agttgaacaa gtaataccag ctgctcccat
     5101 atcttgcata ccaataacgg cacccgtttt aataacttct aatgttgctt ccaacaataa
     5161 tttctctaca aatggatcgc ctacttgtac agaaggaatc ttatcttcag catcttcgcc
     5221 taaatcctcc gaagcaaaag tagctccatg aataccatct tttccagttg cagaaccaac
     5281 aatatatact ggatttcctt ttccgtaaga agtagcagaa atagtatctc cgattttcac
     5341 aattccgacg ctcatcgcat ttaccaatgg atttacttgg tagcaatcat caaaatatac
     5401 ttcaccagct actgttggaa caccaaaaca atttccataa tgtcctatac cttttacaac
     5461 acctttcaat aagtgttgtg tacgtttatt atcaatcaat ccaaaacgca atgaatttaa
     5521 agaagctatt ggtcttgctc ccatagtaaa aatatcacgc gaaataccac ctactccagt
     5581 tgcagctccc tgaaatggtt ctactgcact tgggtgatta tgcgattcaa ttttaaaagc
     5641 acaagcccag ccattaccga tatctactaa tccagcattc tcttcacctg cttccactaa
     5701 caaagcctta ccttttcttg gtaatttttt taaatatttt atggaatttt tatacgaaca
     5761 atgctcactc cacatcacag aataaatact taattctgta tagttgggta ctcttcctaa
     5821 aatctcttgg attttaataa attcctcagg taacaatcct aaagttttgg ctaattcttc
     5881 gtttacgata acagacataa taaaattgat ataagttaca aaagtacaaa ataaaccact
     5941 ataagactaa aaatatttgt tatttagtgc agttttatta atttcgtaag agtttattat
     6001 acttatgttg ctggaaattt tatacccttt actttttatt cttttattga gtgtaattat
     6061 ttctaaatcc aaatacttta aaagcactga aataggttct ccaatattag ttttgggttt
     6121 cttattgaaa tgtataagtg ccttttattt tggctattta tttaataaga acattctaac
     6181 aggaaatgat acttttaatt atttaagaaa ttcagaaatt atatattctt ctatacaaga
     6241 atcatggctt acctatttta aactaacatt tgggcggaat tggcattcca atccagactt
     6301 aaatctcata ccatatatag ataatatgga tttttggtac gacaatagta attatttgat
     6361 ggtgcgaata aatgctatta ttcgattatt ttcctttgga atttacaacg tacatgccgt
     6421 aatttttgca tttatttcat ttattggtat ttataattta tacctctttt ttgaaaacaa
     6481 agtttttaat aaaaggattt tacaaataat tttgtttggc attccttcta ttgttttttg
     6541 gacttctggc gttcataaag aagccatagt tatctttgca ttaggtatga gtttatataa
     6601 tacagataag ctttttagca ctcaatacga aaggaaacac gttattctta ccattttagg
     6661 attatttatt ttaggaagca tcagaatcta tatacttgca tttttaattc ctttgtatat
     6721 tgcaattatt ctttctcaga aaatcagctt ccccaaacga acatttcgtt tttatgccgc
     6781 ccttactatt ttcttatgta ttattagttt ggcattaata gatacgctaa ttccaaactt
     6841 tagtttgttt aatgaattca tcgttcgaag aagtcatttt ttgagaattt ctggcaatac
     6901 cacatttgat gttgttacaa atttcaatag tattgaaggt tttattatgg taataattga
     6961 tgcgattatc aatccattta ttcatccatt tccaaatgaa tctaatttat ttttagtatt
     7021 tattgcttca ttggaaacat acttaatgtt gtttataatt ttagccacaa ttttctcttt
     7081 aagttacaaa aatttataca aaaatcctta tgctttgttc tgtatattat tctcgctcag
     7141 tatattattt ttaatcggtt taattgtcaa taattcagga gcaattgtca gataccgttc
     7201 aattgccata ccatttttat taattggctt gtgtttatta tacaaaccga aggaaagttt
     7261 acaactagaa acagaagttg gtataaccaa ataaatttat agattttaca aacattttgg
     7321 caaaatatat tgcagtttag tccatgcttt ttcattatca tgcaaaacta aaatatcatt
     7381 ttctctaaga tgattttgaa tgttttcata acacttttca ggtgtaactt tgggatgata
     7441 atcatgtggc aatacagaac aaatcacgat ttctcctttg gtaaatttgt ccattgcacg
     7501 aacttttgca taaggtggac gaaataaatt actcccacta atagcttttc ctttttgata
     7561 attttctaag taattctttc tggaaattct ccacccatta taatgtttgt agccgtggtt
     7621 gccaatggtg tgtccatctt ttttaatctg ttcaaatatt tcaggatatt tttcaatatg
     7681 ttcacctaaa caaaaaaatg ttgcttttac attatttgct tttaattgtt ccaaaaccca
     7741 aggagtaatt attgggtgtg gtccatcatc aaatgtaagg tatatttttt tgcccataaa
     7801 agtacaacta ggcattttaa ttaacgctct agcctcttca ttcatttccc aaattacctt
     7861 tggaaatgat ttttgtataa tatttggtgt tcttacccaa aacatactat ctattttgtt
     7921 aaacaaattt ttaatctaat catttttttc acagagtctt ctatttgata cgctaatgct
     7981 gtttgcttat ccaactcctc aactatctgt tcggacaata atctcggatt tttaggcatc
     8041 aaaacaatat cattcccagc ttctaaagct ttaaaatcgg catttggaat agtagaaacg
     8101 cccaacatat tcattgcatc tgtaataaca attccacgaa atcctaaatc atttttcaat
     8161 aaattagaaa caatattttt atcaatactt gatggcaatc cagctgtatt aaactgttca
     8221 ttatctttaa tcgctatatg acccatcatt gtaaaaacgg gttcagactt ttggattaag
     8281 tgataaaaag taggtacttc ttttaaaggt ccattaatat atactaaatt tttatgtgaa
     8341 tcgccaatta catttccatg tccagggaaa tgtttaattg ttgttgcgat attgaaagtt
     8401 tttgactcat caataaattg tgctgctcta tgaataatat catcaatatt attaccaaac
     8461 gagcgattat taattactgc tgcattaaac gcattatctg ccaatggtga taaattaatc
     8521 tgaataccta ttttatccaa aaccgatgaa attgttttag ctgcatctaa gactaaacta
     8581 tcagaattca attgactggt ctttaaaaca ttcggagagc caaaatactt catattaaac
     8641 aatgaaggtt cacaatcaca ggcataaata ttattaaaac tcgtttgaat tgcattataa
     8701 tccttaattt gattggctat atttgataaa taacctttca aaaaaataac attactaatt
     8761 attgtatctt ctaatagttt tttagaatta ttataagtat aaacattatc tgtagagctt
     8821 acagcataca tgataatttg agctgctttt tgtttgttat ttaaggaatt aaaaacagaa
     8881 tcagtaacat aatccaaacg tttattctct ttataaaaat cattaataga aaatgaatta
     8941 gcataaatat tcaagctatt taccaaacaa agccaaatta ttatccaaaa cttatgcatt
     9001 aatttcttat ttattttatt actacaaaaa tcattctact tttgaagcaa aaatttcctt
     9061 ttttagattt ctattatgtt ctatagctaa aacacagtca taagttgctt gtcttaaagc
     9121 taattctctt gggtcattcg caatatgtgt tcccatttta ttcatcacat aagcaaaagc
     9181 aactttcatt gttggatcgg caaacgcacc agagccacca gcaccaaatg taccaaatgc
     9241 tttataatca attccaaaat tttgataggt tgaaggtttt gcaaaaccaa ccgaaaatgg
     9301 gatatttaca cctaatacca aatctgttct tctgattttt ggagcaatcg gtgcttttgc
     9361 taattcctcc aatgtttctt tacgaatgcc taacttctta cctcctgttg caaattcatt
     9421 ataaattgta gctaagccac gagcatttga aaaaccaaga gctgcaccat tttgaatttc
     9481 tatatatttt cgcttattaa aattatttag gttgatagca aaaggtggat ttaataaaga
     9541 tttataaaac atagaaaatg gattggtaat atttttaatc aacttcccaa aatctctatt
     9601 atcacctcca gttattgcct ctaatggatt gaatggtata ttatctgctt tgcgttcaat
     9661 attgatttcc tttggaaacc caatatacat ttcaacaccc aaaggatgag caatttcttc
     9721 ataaacaaac tctgataaat tacgaccttt agggtctata cgcttcatta attcattctg
     9781 gtacatagca attgtccaag cgtggtaacc ttgcctatct ccgactttcc aataaggttt
     9841 ctgctttgcc aaagcagaac ttaatgcctt ttcatctttt aataattcat aactaaatga
     9901 tttatcatta ctaaacacac ctgcttgatg agcaaataat tgtctaactg taatattttc
     9961 ttttctattt acagcaaatt ctgcccaata ggtagctact ttttcatcgt aatctaataa
    10021 tcctcgactg tgtaatattg ccattgctaa tgaagaaata cctttggttg ttgaaaacaa
    10081 caatgctaaa gtatcttctt cccaatgttt tcgtctgtct ttgtctttat aaccagcata
    10141 caaatctacg accttttcgc cttcaaaata aacacaacaa ctcgctccta cttcttttct
    10201 atgataaaaa ttaaattgga agacatctct tactttctca aatcctgctt tagtgtaacc
    10261 accaatttgt atcggttctt tatatgccat aagtaaaatt aaatattttg ttctaaaata
    10321 agaaatacta agagaaatgc agcattcact gagtaataat tatccttaga gaaatattta
    10381 aaaaatattt tctacttcaa taaagcagtc aattcatgtt gaatgctagc ggttttgtct
    10441 ttattataaa agtgttgcaa tgcttcttta attttttcaa atggctctcc atttgccttc
    10501 tgttcattga atatattttg tgcattgact ggtcgtggac cccaacgaaa tttatcttta
    10561 ccatctgcat ctctaactat aaaaattggg atagattttc ctccatttgt taggtaatta
    10621 tcaatttcag aatcggtatc tcgcaattgg agttccaaat gaatattttc attcaaaatt
    10681 gacatcatat aaattatcgg tgtaatatga gcagcatcgc cacaccatgg ttctgttatc
    10741 aatatccaat tttgcttttc tttaatggca tgaattacgg cttttgtttc ttcagttagt
    10801 acagcagttt tcaaccatct tttcaaccgt gaatgattca attttgtata ttcaaaatat
    10861 tctgggtcgt tatatggtgc agttgggttt tcatcgttta aaatttgttc aaacttaatt
    10921 aagtattcat tccaattcat aaaagtatta ttataattat ttaattttaa aattcaattt
    10981 ataatggttt ttgagcatgt aaaggtcgca ttattatttc atctattagc atattatcag
    11041 gtgtttttag gatatgaatg attgtgtctg caatagcatc aggttgtaac atattagaat
    11101 gtttttcaat acctgaattc ttaaaaaaat ctgtttctat tgaaccagga tgcacacagg
    11161 ttactttaat atcaaatgga cgtaattctt ttaataaagc ttcactaaaa ccacgaacac
    11221 catatttagt cgcacaataa gcagttccat cttccctacc cattgttgct aagatagaac
    11281 caatattaat aatatgagat acttgtttat tcatttttag aaatggaaca atcaatgagg
    11341 ttataaaata cataccattc agatttgtat tgaccatttc caaccacttt tctgtgggaa
    11401 tttcatcgac tttcccaaaa ttacctatac ctgcattatt tactaagata tcaggaagat
    11461 gcttttcatt aaacgttatt tgaatccaat ttaacaatgc ctctcgattt gtaatatcca
    11521 tttcaatagg atgaaataaa taccccaaac tttcttgcaa attggatagt gctactttat
    11581 ttctcgctaa accataaacc aaaacatcac tttttacgag tgcttttgca atggctacac
    11641 ctaaaccact acttgcacct gtaaggatag caattttatt ttttaaaaac acaattttag
    11701 attatgggtt ataagaacct gcaacagttc taaaaggtat tgctaatcgg ttccatccat
    11761 taatagcaat aattgtcatt gttaaggcta ttaaatcttg ctcattaaat tctttgctta
    11821 ctcgttcata caactcatca gaaataggat ttccagcaat taaagttaat gcctctgtcc
    11881 attctaaagc aatccgctca cgttcagtaa aaaaaggcgt ttcacgccaa gctgaaagag
    11941 catataatcg ttgctctgtt tctccttctt ttcttgcatc tttggtgtgc atatccaaac
    12001 aataaccaca accattaatc tgcgaagctc tagtttttac taattcaaat aaacttttct
    12061 ctaaatttga atgatgaata taattttcaa tttccaataa gccttttata gcttttggtg
    12121 ctacttgacc ataatctaat ctacttttca tttttaatct tttttcaaat aatctctcaa
    12181 tacatactgc aaaataccgc catttttata gtactcaatt tcaatatctg catctaatct
    12241 tgcttttact ttaaaatcaa tttcttttcc tgatggatgc actgctttaa cttctaaaat
    12301 tttatgagga actaaatcat tttcaaatcc aataatatcg aaaacttctg aaccatctaa
    12361 tcctaaagat tgtgcatttt cgccatccat aaatactaat ggagcaacac ccattccaac
    12421 tagattactt ctatggattc gttcaaaact ttctgcaatt accgctttta tacctaataa
    12481 aacagtacct tttgctgccc aatctcttga tgaacctgag ccatattctt ttccagccaa
    12541 tacaattaat ggtgttttgt ctttttgata agccattgct gtttcaaaaa cagtttttac
    12601 ttctccagtt ggaaaatatt tactaaaacc accctctttg tcaattattt tatttttgat
    12661 acgcacattt gcaaaagttc cacgcatcat cacttcatga ttacctcttc gagaaccata
    12721 agaattaaaa gccacttttt caacaccatt tgcaattaga tattgcccag ctgccgtatc
    12781 ttctttaaat gaacccgcag gagaaatatg atcggtggta acagaatcac ctaaatacaa
    12841 caagactctt gctgatttaa tatctgtgat tggtgatagt tgattgctaa tattttcaaa
    12901 gaatggagct tctttaatat aggtagaatc atttctccat tcaaaatttt gatctagttt
    12961 tactttcaaa tctttccaat cttcagagcc ttcaaaaatt acatcataaa ctgctttaaa
    13021 atcctcttgt tttacacagt cattgattgt attttgaatc tccacacgac ttggccaaat
    13081 atcttttaaa tatacaggct caccattcgg gtcaaaacca agtgcatcat gcattaaatc
    13141 aacatctaca cgacctacta atgcataagc aacaactaac attggcgaca tcaagaaatt
    13201 catttttgtt tgagggtgca ctcttgcttc aaaatttcta ttacctgata gtacagatgc
    13261 aactaccaat tctccttttt caatggcttc cgcaatatgt ggtggcaaag gccctgaatt
    13321 tccaatacaa gaagtgcaac cgtacccaac tatatgaaat cgaagtgcat ctaaatcata
    13381 atttaagcca gaacgttgca aatattgagt aaccactttt gaacctggtg ctaaggacgt
    13441 ttttacccac gatttagtac gcaaccctct ttcaattgca tttctagcta acaatccagc
    13501 tccaatcatt accgcaggat ttgaagtgtt ggtacaactt gtaatcgctg caattgcaat
    13561 gcttccatcg ctaaaattat attcttgatt tttattttta atacgaacag tatgcaaatc
    13621 ttctctaaca atctcagcat ttgaggtatg tccgtcgtgt ggaactttac caaataaaaa
    13681 ttctgttcct gagccacctt ccgccaacca agcagcttct tttcgctcat ttacttgcgt
    13741 ataagttctg ttatattctt gctgtaataa atcagcaaat ttatttttca aatcttttac
    13801 aaaaatttta tcttgaggac gtttaggtcc tgaaactgtt ggttctaaac tattcaaatc
    13861 aaactcaaca actgaggaat atttaatctg ttcattacct gttctccaaa gtaaattctc
    13921 tttacagtat ttctctacaa tattaatttg ctcttcagaa cgattggtat ttcgcatata
    13981 ctctaaagtt ctgtcatcaa taggaaaata agttacagta cacccaaatt ctggcgacat
    14041 atttccaatt gttgctctat cagtaacact taaattattc aagccctcgc caaatacttc
    14101 tacaaattta ccaacaacac ctttatctct taaaattctt gtgatggata aaaccatatc
    14161 agttgcagta cattcgttgg gtatttttcc tgttaatttc aaaccaacaa cctctggaca
    14221 tgtaaagaaa ataggctgac cgagcattgc tgcttctgct tcaattccac caacacccca
    14281 agcaacaact ccaattccat taaccatagg cgtatgtgaa tctgttccaa ctaatgtatc
    14341 tggaaataac caaccatcac gattggtaat acctttagcc aaatattcta aattcacttg
    14401 atggcaaatg cccattccag gaggaactac tgtaaaatta ttcattccat tttgagccca
    14461 ctttagcaat tcatagcgtt ctttatttct ttcatattct acttctacat ttttttcata
    14521 agcataattt gttccaaaat aatcaacctg aactgagtga tcgataacta aatctactgg
    14581 aattgcagga ttaatttttt gtccatcttt tccatgtctt acaaattctg aacgcaaaga
    14641 agcgatatca actacggctg gcactcctgt aaaatcttgc attaaaactc gtgaaggcat
    14701 aaaaggaata tccttatctt ttggttcagg tgtccaattt gctaaagttt gaatatgttc
    14761 atcggtaaca ctaaaaccat cgtaattcct cagcacattt tctaataaaa tacgaatact
    14821 aaagggcaac tgttcaatat ttcctttggg aaaatctttt aatgaacaat aattgtaatc
    14881 aacaccattt acctgaatta atttttggaa ttttggagat tttgtactca taaactttta
    14941 ctttattgat gattaataaa tacttatctt aaaaagttga agtaaggtaa tatactttaa
    15001 aaaataagta catataacaa acataattat tattcaaaat gtttagtact tgacaaaata
    15061 atccgaatat attatagcga aatataaaaa ctttcttgtg cttaaatagg tttacacatt
    15121 aataaccgaa gtataacact ttttttcata aattccatca actatttatc agtacattac
    15181 ctgcattaaa ataaaatgtt tatttatcat agggtttgaa acaaaaaact gtttttaggg
    15241 ggacaagata aatcataaat catctaaagg acttttgact ttagctaact gtttgttcgt
    15301 tactttggca tatatttggg tagtccgtac gtctttatga cctaataact tttgaataaa
    15361 aaccatatct gttcccgctt ctaataaatg tgtggcgtaa ctgtgcctca agccgtgaat
    15421 accaatggtc ttctttattt tggctttttt cattgccgtt ttgaatatag cttgtacact
    15481 gcgaatagaa tattgaccgc catactgtcc ttcaaaaaga aattctttag gacgatattc
    15541 cctaaaataa tctcgtaagg tttctaaaat agtttcggga agcggtacat aacgatcttt
    15601 tttacctttg gaattttcga tatgtactaa catacgtcca ctgtcaatat tagagatttt
    15661 aagatttacg atttcgctga cacgtaaacc cataccataa catagcttta gaataatctg
    15721 atgtttcaga ttatccactt gtccgaaaag ccgtaaaatc tcatttttgc ttaacacttt
    15781 gggaagagat gttttttgct tgggtcgtgg aatttcttga aaaaatagtt tttcatattt
    15841 cataacctgc tcaaaataga actttatcgc attcatacga ctatttagtg tattttctga
    15901 caacttgagt ttttttgtac agtaaagcat ataggaacgt aggcgttcag atgtaagtgt
    15961 attcacatcg atatttctaa gcgtaacaag cagttgcata aactcaccac agtagctttt
    16021 aatggtattt tgactgtacc cttttaataa aagcgtttcg tgcatacgtt gtaaagcagg
    16081 acgattaact tcagtaatca gttcaaaatc aactttttca aatgatgttt tttcgtgcaa
    16141 gccaagctgc tctctgattg ccttaacatc attgagatac cagcattttt tagacccgct
    16201 gaactgtact gatgggaagt gttgctttag ttcatttctc aaagtagcat catttggaaa
    16261 cagtatccaa attacatttt tgtccctatg ttgccctatc tcaaatttat atttattcag
    16321 t
//
LOCUS       DAOQCW010000051        16119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1290_length_16119_cov_4.765999, whole genome shotgun sequence.
ACCESSION   DAOQCW010000051 DAOQCW010000000
VERSION     DAOQCW010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16119
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1290_length_16119_cov_4.765999"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="PLS16_06020"
     CDS             <1..1249
                     /locus_tag="PLS16_06020"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNJ60671.1"
                     /translation="DIITGLDAELQAYGEKLMTGKVGSIVAIEPSTGQILAYVSSPTF
                     DPNLLSGRYRQNNFNYLFRDEDKPLLNRPISAMYPPGSTFKVGAALVMLQQNTHPWNW
                     SWFCPGGYRVGSHFLRCHGTHAIPDVQTALQHSCNSYFCKIYNDFVMDSTYATPEIGY
                     QKWWDYMFDLGYGKLTGIDLFGEKKGNLPTTNYYNKIFGKGKWRAATTISNAIGQGEI
                     LSTPLQICNEMAMVANHGYYWEPRVAAYIIKDGKKYKVKQKKIIVGIAPEHFPLVIDG
                     LEKVVEQGTGRIARIEGLTVCGKTGTAENPHGKDHSIFSGFAPKVNAKIAIAVFVENA
                     GFGATYAAPIASLMMEKYINDSISKKRLFLEERMIKTHTIKPKIDTNYIKSKTYIDSL
                     QRIKHKNDSIQKLKAKKDSTKTN"
     gene            1250..2527
                     /gene="rodA"
                     /locus_tag="PLS16_06025"
     CDS             1250..2527
                     /gene="rodA"
                     /locus_tag="PLS16_06025"
                     /inference="COORDINATES: protein motif:HMM:NF037961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNJ60672.1"
                     /translation="MKADYKKNSGSIDTILLLCYIALVVIGVLSIFSATYNNEFQGTF
                     FSLSHSYMKQVLWAGISFLMFFALIGMDRRIIQFFSYPAYILIMLLLFSVLFIGERTK
                     GDQNWINLGFFKLQPSEFAKFATAMALAAFFDTNNMHFKTTKDYLTAFAIIAIPLGLV
                     LLQGDAGSAIVFLSFIFVLNREGLPDGLIYFGLYVVFIGILSLVLDKFIIIAITIFVF
                     GIFIYINLRNKAMVKFLAIIMFGTMIFSSSVSIIFNNVLLKHQRDRINIVLGKELTKE
                     AERGIAFNLNQSKIAIGSGGIIGKGYLNGTQTRFNYVPETSTDFIFTAIGEEWGFLGS
                     LVLIGLYVSLLTRLIFMAEQQRTAYGRVYIYSVAAIIFTHVAINIGMTIGIVPVIGIP
                     LPFISYGGSSLLSFSFMIATCLKLDNEQRGIMR"
     gene            complement(2535..3134)
                     /locus_tag="PLS16_06030"
     CDS             complement(2535..3134)
                     /locus_tag="PLS16_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60673.1"
                     /translation="MNNKQSRILNIITFLAFTILGIYKNEVTVFYIIYLFWMEAFVRQ
                     LIELSYIIRRDSKLFSSISVAWPAFFMMIIYVVFIIVLFGFIPFSAGKDSETFLINVK
                     TLMFKNIFFNLSVLVYIIQYILYIYVNGFKEKTIIPFNRNHIILHISIILGAVIQLTP
                     IVSFVKPKELINILSIIPFLILKIILDKGFKSKGESILV"
     gene            3274..4956
                     /gene="ilvD"
                     /locus_tag="PLS16_06035"
     CDS             3274..4956
                     /gene="ilvD"
                     /locus_tag="PLS16_06035"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNJ60674.1"
                     /translation="MAELNKYSKTITQDPTQPAAQAMLHAIGMTNDDFKKAQIGICSV
                     GWDGNPCNMHLNGFGHELKGYVNQQADLIGLSFYTIGVSDGISMGTPGMRYSLVSRDV
                     IADSIETNAGAQYYDGIVSIAGCDKNMPGVIMGMARLDRPSIMVYGGTIGAGHYKNQQ
                     LDVVSAFEALGQKFAGSISEEDFKGVIESACPGPGACGGMYTANTMASAIEAMGMSLP
                     YSASNPAISEDKKNELKDVAAAIRNLLEKDIKPSDIMTFKAFENAIRIVFVLGGSTNA
                     VMHLIAMAKAIGVKVTLDDFVRLAEITPVLSDFRPSGRYVMEDLHKIGGVPAVMKLLL
                     KEGMLHGDCMTVTGKTIAENLADLSELSDGQDLVYPFSNPIKKTGHIRILYGNIATEG
                     AVAKITGKEGERFEGEAICFDKEQDAIDAMQAKKVKAGNIVVIRYVGPRGAPGMPEML
                     KPTSVLMGEGLGKDVALITDGRFSGGSHGFVVGHVTPEAQNGGNIALIKDGDKIIIDA
                     VKNTIDVIVSDEILTERRKALQLPELKAKKGVLYKYAKTVASASEGCVTDEF"
     gene            4985..6079
                     /locus_tag="PLS16_06040"
     CDS             4985..6079
                     /locus_tag="PLS16_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HNJ60675.1"
                     /translation="MRKLNFLLMFVISVAFISCKNEGKANQEVASAEDTELLQTAKNL
                     FKPITGNVDEEGKKNLSTEMVELGKTLYYDTKLSPKGNNSCNSCHNLNTFGVDNLSFS
                     PGDEGKLGGRNSPTTLNAAFHSTQFWDGRAKDVEEQAGGPILNPVEMNMPNEKTVIDR
                     LSKVDYYKDLFKKAFPNDANPISYKNIQTAIGAFERTLVTPSAFDKYLADDHNALDAQ
                     QKKGLDLFIKTGCISCHSGVALGGNSFQKFGVFADYRTFTKSNSTDKGLADLNKNEAS
                     TDFFKVPSLRNIEHTYPYFHDGSVKDLNTAIHIMAKAQLNKELNTEEVGDIAAFLKSL
                     TGEVSAYAKTPPAILGDDAIPANKRTTVSK"
     gene            6180..7052
                     /locus_tag="PLS16_06045"
     CDS             6180..7052
                     /locus_tag="PLS16_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HNJ60676.1"
                     /translation="MGASIFTVLDDIAVLMDDVAITAKIATKKTAAILGDDLAVNAEK
                     ATGYVSSRELPVLWAISKGSFINKLIIVPLILILNIYAPFLIKILLVIGGIYLAYEGA
                     EKIIDFLFHKNHKEVENTLTTEGETEEVLEKKKVNAAIKTDFILSLEIVIIALSSVLN
                     ENLTLQIMTVSIVALIATVGVYGFVALLVRMDDLGFQLINVSKNNKFLTILGKGLVNA
                     LPLIVKLLSIVGTIALILVSGGIFIHYIDIFHHLLPKVPIVFREIIFGLGVGIITVII
                     ANLLNWIVKQIRNK"
     gene            7187..>8232
                     /locus_tag="PLS16_06050"
     CDS             7187..>8232
                     /locus_tag="PLS16_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60677.1"
                     /translation="MKQLHFILPVLLFIGISILPTTCHAQFGKVINKVINKTVPNKSS
                     NTTNDGSSNKTNNNSNNSDNIPTIKSNSPIPENEKYNCQEKEVSNGYSSEVHKKYSNK
                     LVFSKTEIVMSNQNEATFSNTFTLKDNIYSRIYLSESPVYTAQTMGLCCKGGLYCMKL
                     FYRYIIDNKETNNQYAVLSTPEPKKITWQLGISPSKEDVNSGYPLREIYKFKELASEL
                     SEGTHKIRVEYFMEIPDDDTERGLLYSTKFGKEIVLASGEFTLQVKTADKTEMKEKLY
                     VKPAPVTYSSSSSNQQSCYVEVKNGSGESIKFDLYTSASRSTESLSSNSNKSIDCNMY
                     EKIVITDGRCKDVT"
     assembly_gap    8233..8332
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8406..10427
                     /locus_tag="PLS16_06055"
     CDS             8406..10427
                     /locus_tag="PLS16_06055"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNJ60678.1"
                     /translation="MKLKFIITLLTFLFFLNIDAQNPDFEIRRNAYIENGFNHLNSNS
                     IIFQAYKNSPIDTTLLNEILNNIASNTTADFDIVKLIRILFLSNGEYDNLILPILEPI
                     PFWLTKDEKLRVYWSENHMIMWMSSNWLLHEKYGKQVDDSLNSQLKHLLKLKVDYGFY
                     EFFSSVYLPYSLSGLLNLADFAQDVEIKHLATQAAQKLLKDILLLTNNQGVFFPTAGR
                     NYYSKYSTPYNQNHSNLIYLLTGMGEEPTSVSHSGSFLATSSIDVNSVISSWTSTLNT
                     NFTVGHSLLDGVNTINKNIPLLSKVIFQWSSGAYFDPDVALSTATILNEYNLWNHDEF
                     KDFKDFKGLPLAIAPTLARIASSISKSSGIYNPNISIFKNNSVTLSSVQDFWKGKNGY
                     QQFPVVANTGTSAVFPISGKVTEDLENRSELHANTHLPYVKQTDNIALVMYRPEKGLA
                     LFGYTDNKLDIALKWDANTFDEIREYNNWILGREKDGYIAVRRHCTDTILNKIACDNH
                     DGQTWIIVVGNIEMYGSFDNFENIIKTSIFESKWYFDLPTLQWAYQSKISIDNKVLEY
                     TWYADIFSFPTEKEVSTAIINKKDYQEISVYPNPANTFVKLSFPNSITKNSRYKIFNT
                     VGETVLEKVIEPTELNNLLINTSTFSNGLYFINIETKDKIYTGKIIIQK"
     gene            10534..11766
                     /gene="serA"
                     /locus_tag="PLS16_06060"
     CDS             10534..11766
                     /gene="serA"
                     /locus_tag="PLS16_06060"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNJ60679.1"
                     /translation="MSKEVSYPKEKIKILLLEGLHQSATNEFNKKGYTNIDSYKEAMT
                     EEELLNCIENYHIIGIRSKTNLTAPVLEKAKKLMTIGCFCIGTNQVDLAKATELGIPV
                     FNSPYSNTRSVAELIIANSIMLLRGVPSKSKAMHEGNWQKTAKASYELRGKTIGIIGY
                     GHIGSQVSVLGESMGLKVIYFDIEPKLPLGNAQAVETLEELYAKADIVTLHVPATPGT
                     KNMITANDFNQMKDGVIFQNLSRGNVVDVDALRDAIVSGKIGGAAVDVFPKEPKQNGP
                     GFESPLVGLDNVILTPHIGGSTQEAQENIGTEVGSKLTSYMDNGSTLGSKSVPELSLA
                     PQKDTKRVLHFHKNVPGVLSEINTTLSKHHVNILGQYLKTNESIGYVVLDVDKNADLT
                     LIDDLKTVANTIKARILY"
     gene            11843..12208
                     /locus_tag="PLS16_06065"
     CDS             11843..12208
                     /locus_tag="PLS16_06065"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNJ60680.1"
                     /translation="MPYKEKEIEKLYYSIGEVASMLDVNKSLLRFWETEFTNLRPKKA
                     SKGNRLYTKQDIDYIKKIKSLVKDKGFTIEGARKQLKEKQDIIINNNIYVKNDFDKNE
                     VSKKLNEIKFFLSELAKKI"
     gene            12210..12749
                     /locus_tag="PLS16_06070"
     CDS             12210..12749
                     /locus_tag="PLS16_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60681.1"
                     /translation="MPKNIQYFIFSFVFFIACKSDKIVIKNPTVFFQELIADDKNAIF
                     KNINFDFSKDDVIDAEKSQQYDATESYLAYSYNFPKDTSKFVEYADIKYFFDEHDVLK
                     IITVNVYLNDSIQETQLLDNFKEYYSHKYDAPKIDEYQYYSWDATADSKTTKGKYYFN
                     IGIRKLAGEYGLALEFLRL"
     gene            complement(12774..14234)
                     /locus_tag="PLS16_06075"
     CDS             complement(12774..14234)
                     /locus_tag="PLS16_06075"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNJ60682.1"
                     /translation="MSPTLILACILVYFLCLIGIGYITSRKANNASYFIGNKESVWYI
                     VAFGLIGDSLSGLTYLSVPGSVKAAQFNYFQVVLGYVLGYWAIAYFLLPLYYKHNLTS
                     IYTYLDKRIGTISQRTGSFYFILSRLIGAGGRLFLASIVLQKFVFDGFGIPFPVTVAL
                     IINLMLVYTLKGGIKTLVWTDMFQSIFLLMGVLFTIVFIMQRLDWGVAEVFAQIKNSD
                     YSQVFFWDWKAKSYFWKQFLGGAFIAICMTGLDQNMMQKNLSCKTLNEAQKNINSFSI
                     IVVIVNVFFLALGALLYLYANQLEIPLPEKPDQLFPTLAFNHLGQAAGLVFIIGLTAA
                     TFNSADSVLTTLTTSFYIDFLHKDETSSDANKKLRYVIHITFAILLLFVVLAFGLFDD
                     QSLVFTVLDIAGITYGPLLGLFIFGMFTTRRIKDIYVPIICIISAGFCTYLYFSSNHN
                     ASWLNGYKIGVELLIINGFMTFVGLLLFSNKSKEIY"
     gene            complement(14320..15516)
                     /locus_tag="PLS16_06080"
     CDS             complement(14320..15516)
                     /locus_tag="PLS16_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNJ60683.1"
                     /translation="MFKNIKLKRSVYATEEHELLQKTTQHFIEKEIAPYHAQWEEDSM
                     VSREVWLKAGETGLLCLDVPEQYGGAGLDFSFCALITEEILKAGYSGPGFFLHSDIVA
                     PYIVDYGTEEQKKKWLPKMCTGEVITAIGMTEPSCGSDLQALKTTAEDRGDHYLVNGQ
                     KTFITNGYMSDLAIIAARTIVDGEFIGISLFLIEADRAGFSKGKPFKKVGMKAQDTCE
                     LFFDNVEIPKDNLLGEIGKGFVYMMQKLGRERLSVALMAVGGAEGALENTIVYTTSRE
                     AFKQPVAAFQNTQFKLAELATQLQIHQTFVDRCVELYCNKELSAETASMAKYSCTDMH
                     QHVVDECVQLHGGYGYMWEYYIARAYADNRVARIYAGTNEIMKLLISRGLLREMHEAN
                     KRAAKK"
     gene            complement(15539..>16119)
                     /locus_tag="PLS16_06085"
     CDS             complement(15539..>16119)
                     /locus_tag="PLS16_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNJ60684.1"
                     /translation="GSFIGLAATTQSTIRIKNTRLDMLGLIPKYFEKLGIQLKIENDD
                     IIVEEQETYEIQSFIDGSVLTIADGPWPLFTPDLISIMLVTATQAKGSILIHQKMFES
                     RLFFVDKLIDMGAKIILCDPHRATVIGINREYQLRGINMSSPDIRAGVALLIAALSAK
                     GQSVISNVHQIDRGYQFIDKRLQAIGANIVRK"
BASE COUNT         5796 a         2355 c         2524 g         5344 t
ORIGIN      
        1 tgatattatt actggattag atgcggaact tcaagcttat ggtgaaaaat tgatgactgg
       61 aaaagttggt tctatagttg ctatcgaacc atcaactgga caaatattag cttatgtaag
      121 tagtccaact tttgacccaa atttattatc tggaagatat agacaaaata atttcaatta
      181 tttattcaga gatgaagata aacctttatt aaatagacca atcagtgcaa tgtatccgcc
      241 aggctcaact tttaaagttg gtgcagcttt agttatgtta caacaaaata cgcatccttg
      301 gaattggagc tggttttgtc caggtggcta tcgtgttggt tcacattttt taagatgtca
      361 tggaacacat gctattcctg acgtacaaac tgcattgcaa cattcttgta attcctattt
      421 ttgcaaaatt tataatgatt ttgtgatgga ctcaacttat gccacgcccg aaattggcta
      481 tcagaaatgg tgggattata tgtttgattt aggttatgga aaattaacag gaattgattt
      541 attcggagaa aagaaaggga atcttccaac gacaaattat tataataaaa tttttggtaa
      601 aggaaaatgg cgtgccgcaa caactatatc caacgcaatt ggtcagggag aaatcttatc
      661 tactccactt cagatttgta atgaaatggc aatggttgca aaccatggtt attattggga
      721 accaagagtg gctgcctata ttattaaaga tgggaaaaaa tacaaagtaa aacagaaaaa
      781 aataattgtt ggcattgctc cagagcattt tccattagtg atagatggtt tagaaaaagt
      841 tgttgaacaa ggaactggta gaattgctag aattgaaggc ttaactgttt gtggaaaaac
      901 aggtactgcc gaaaatccac atggcaaaga ccactccatt ttttctggtt tcgctccaaa
      961 agtaaatgca aaaattgcaa ttgctgtttt tgttgaaaat gctggattcg gtgctactta
     1021 tgctgcacca attgcctctt taatgatgga aaaatatatt aacgattcaa tttctaaaaa
     1081 acgtttattc ttggaagaaa gaatgattaa aacgcatact ataaaaccta aaatagatac
     1141 gaattatatc aaatcaaaaa cttatattga ttcattgcaa agaataaaac acaaaaatga
     1201 ttctattcaa aaattaaaag caaaaaaaga tagtacaaag accaattaaa tgaaagcaga
     1261 ctataaaaaa aatagcggtt ctattgatac cattttactc ttatgttata ttgcattagt
     1321 tgtgattggt gtgctttcta ttttttctgc aacttataat aatgaatttc aaggaacttt
     1381 tttcagtcta agtcatagtt atatgaagca agtactttgg gcaggtattt catttttgat
     1441 gttttttgca ttgattggaa tggacagaag aattattcaa tttttttctt atccagccta
     1501 tattttaatc atgttgttgc ttttttcggt attatttatt ggagaacgta ctaaaggtga
     1561 ccaaaactgg ataaatcttg gtttctttaa attacaacct tctgaatttg ccaaattcgc
     1621 tactgcaatg gcgttggcgg catttttcga taccaataat atgcatttta aaactactaa
     1681 agattactta actgcatttg ctattattgc aatcccatta ggattagttt tattacaagg
     1741 agatgctggc tctgccatcg tatttttatc ctttattttt gtcttaaatc gagaaggttt
     1801 acctgacgga ttaatctatt ttggattata tgttgtattt attggcattt tatcattggt
     1861 attagataaa tttatcatca ttgcaattac catttttgtt tttggtattt ttatttacat
     1921 aaacctcaga aataaagcaa tggtcaaatt cttagccatt attatgtttg ggacaatgat
     1981 attttcttcc tctgtgagta taatattcaa caatgtttta ttaaaacacc aaagagatag
     2041 aattaatatt gttttaggaa aagagctaac caaagaagcc gaaagaggta ttgcatttaa
     2101 cctaaaccaa tcaaaaattg ctattggttc tggtggtatt attggtaaag gttatttgaa
     2161 tggaactcaa acacgtttta attatgttcc tgaaacaagt accgatttta ttttcactgc
     2221 aattggtgaa gaatggggct ttcttggttc attagttttg attggattat atgtttcact
     2281 acttactcgg cttattttta tggctgagca acaacgaacg gcatacggtc gggtatatat
     2341 ttattcagtt gcagctatta tttttacaca tgttgccata aatattggta tgaccatcgg
     2401 aattgtacca gttattggaa ttccacttcc tttcataagt tatggaggtt cttccctatt
     2461 gtcatttagt tttatgatag caacttgttt aaaactagac aatgaacaga ggggtattat
     2521 gcggtaattt tcacctaaac caatattgac tcccctttag acttgaaacc cttatccaga
     2581 attattttta aaatcaaaaa aggtattata gatagaatat ttatcaattc cttaggttta
     2641 acaaaactca caattggtgt taattgaatt acagcaccta atataatgga aatatgtaaa
     2701 ataatatgat ttcgattgaa aggtataatt gtcttttctt taaaaccatt tacataaatg
     2761 taaagtatat attgaattat atatactaat acacttaaat taaaaaaaat atttttaaac
     2821 attagagttt ttacattaat aagaaaggtc tcggaatctt ttccagcaga aaatggtata
     2881 aaaccaaata aaacaattat aaatacaaca taaattatca tcataaaaaa tgctggccat
     2941 gctacagaaa ttgaactaaa taatttgcta tctcttctga taatataact taattctatt
     3001 aattgcctca caaaagcttc catccaaaaa agatagataa tataaaatac agtaacttca
     3061 tttttataaa taccaagtat agtaaatgct aaaaaggtaa taatatttaa aatacggctt
     3121 tgtttattat tcacaatatc caaatataac aatatctata aactaaatgc acctaaaaat
     3181 atgtaagtat catttttctt tattgacaat tcaattttat ttatatgaat attaggataa
     3241 atctccttat ttttgtagta aataaattac gtaatggctg aattaaataa atactctaaa
     3301 acgattactc aagatccaac acaacctgcg gcacaagcaa tgttacatgc aattggcatg
     3361 actaatgatg atttcaaaaa agcacaaatt ggcatttgtt ctgttggttg ggatggcaat
     3421 ccgtgtaata tgcacttgaa tggttttggt cacgaattaa aaggttatgt gaaccaacaa
     3481 gcagatttaa tcggattaag tttttataca attggtgttt ctgatggaat ttcaatggga
     3541 acacctggca tgcgatattc tttagtttct cgtgatgtaa ttgcagactc catcgaaacc
     3601 aatgctggtg ctcaatacta tgatggtatt gtttctatcg caggttgtga taaaaatatg
     3661 cctggcgtaa tcatgggtat ggctcgattg gatagaccat caattatggt ttatggtgga
     3721 actattggtg ctggacatta taaaaatcaa caattagatg ttgtttctgc atttgaagct
     3781 ctaggtcaaa aatttgctgg ctcaatatca gaagaagatt ttaaaggtgt aatagaaagt
     3841 gcttgtcctg gtccaggtgc atgtggtgga atgtacacag ccaatacaat ggcatcagca
     3901 attgaagcaa tgggtatgag tttgccttat tcagcaagta atccagcaat ttcagaagat
     3961 aagaaaaacg aattgaaaga tgttgctgct gcaatcagaa atttattaga aaaagatata
     4021 aaaccatctg atattatgac attcaaggca tttgaaaatg caattagaat tgtttttgta
     4081 ttaggcggaa gtaccaatgc agtaatgcat ttaattgcta tggctaaagc tattggtgta
     4141 aaagttactt tagatgattt tgtaagattg gcagaaatca caccagttct ttcagatttc
     4201 agacctagtg gaagatatgt gatggaagat ttacataaaa ttggaggtgt acctgctgta
     4261 atgaaattac ttttaaaaga aggtatgtta cacggcgatt gtatgactgt tactggaaaa
     4321 acaattgcag aaaatctagc tgatttgtct gagttatctg atggtcaaga tttagtttat
     4381 ccgtttagta atccaattaa aaaaactgga catattagaa ttttatatgg caatattgca
     4441 acggaaggag ctgttgcaaa aattacagga aaagaaggtg aaagatttga aggcgaagca
     4501 atttgttttg acaaagaaca agatgcaatt gatgcgatgc aagctaaaaa agtaaaagct
     4561 ggaaatattg ttgttattag atatgttggc cctcgtggtg ctccaggtat gccagaaatg
     4621 ttgaaaccaa cttctgtttt aatgggagaa ggtttgggta aagatgttgc tttaattact
     4681 gatggtagat ttagtggtgg ttctcatgga tttgtggttg gacacgtaac gcctgaagct
     4741 caaaatggtg gaaatattgc tttaattaaa gacggagata aaataattat cgatgctgtc
     4801 aaaaatacga tagatgtgat tgtttctgat gaaattttaa ctgaaagaag aaaagctttg
     4861 cagctgccag aattaaaagc aaaaaaaggt gttttgtata aatatgcaaa gacagtagct
     4921 tcggcttctg aaggttgcgt aaccgatgag ttttaattaa taattcaatt aaaaatatca
     4981 aaaaatgaga aaattaaatt tcctcctgat gtttgttata tctgttgctt tcatcagttg
     5041 taagaacgaa ggcaaagcta atcaagaggt agctagtgct gaagatactg aattgctcca
     5101 aacagcaaaa aacttattca aacctattac tggaaatgtt gatgaagaag gaaaaaaaaa
     5161 tctatcaaca gaaatggttg aacttggaaa aacattgtac tacgatacca aattatctcc
     5221 aaaaggaaat aatagttgta attcttgtca taacttgaat acttttggtg tcgataattt
     5281 atctttttcc cctggagatg agggtaaatt aggaggaaga aactctccta ctaccttaaa
     5341 tgctgcattt cattctactc aattttggga tggtagagca aaagacgttg aagaacaagc
     5401 tggaggccca attttaaatc cagtggagat gaatatgcct aatgaaaaaa cggtaattga
     5461 tagattatct aaagtagatt attacaaaga tttatttaaa aaagcatttc ctaatgatgc
     5521 caatccaatt tcttataaaa atatacaaac ggcaatcggt gcatttgaac gtacattagt
     5581 tacaccatct gcttttgata aatacttagc tgatgaccac aatgctttag atgcccaaca
     5641 aaaaaaaggt ttagatttat ttattaaaac tggttgtatt tcgtgtcata gcggtgtggc
     5701 tttaggtgga aattcattcc aaaagtttgg tgtatttgca gattatagaa catttactaa
     5761 atcaaattct actgataaag gtttagcaga tttgaataaa aacgaagcaa gtacagactt
     5821 ctttaaagta ccatctttga gaaatattga acatacctat ccatatttcc atgatggaag
     5881 cgtaaaagat ttgaatactg ctattcatat aatggcaaaa gctcaattaa ataaagaatt
     5941 gaataccgaa gaagttggag atattgctgc tttcttaaaa tcattaactg gcgaagtaag
     6001 tgcgtatgca aaaacaccac ctgctatctt aggcgatgat gcaattccag ccaacaaaag
     6061 aacaacagtt agtaaataat atgctagctt atataaagta actttgattt tatcccttaa
     6121 atatattatt gaataatgat agatttaagg gataattttt ttaataattt agatgaaata
     6181 tgggagcaag tatttttact gttttagatg acatagccgt actaatggat gatgtagcca
     6241 ttactgcaaa gattgcaaca aaaaaaactg ctgcaatctt gggtgatgat ttagctgtaa
     6301 atgctgaaaa agctactggt tatgtatctt caagagaatt gcctgtatta tgggctatca
     6361 gtaagggttc ttttattaat aagttaatta tagttccgct aatcttaatc ctaaatatat
     6421 atgcgccttt tttaataaaa attttattgg tcattggagg catctattta gcttatgaag
     6481 gtgctgaaaa aatcatagat tttttatttc ataaaaatca taaagaagta gagaatacat
     6541 taactacaga gggtgaaaca gaagaagttt tagaaaaaaa gaaagtcaat gctgccataa
     6601 aaaccgattt tattctttct ttagaaattg taattattgc tttaagttct gtattgaatg
     6661 aaaatttaac tttacagata atgacggttt ctattgttgc acttattgct acggttggtg
     6721 tttatggatt tgttgctctt ttagtaagaa tggatgattt aggatttcaa ttgattaatg
     6781 tgtctaaaaa caataaattt ttaaccattt tagggaaagg tcttgtaaat gctttgccat
     6841 taattgtaaa attacttagc attgttggaa caattgcatt gatattagtt tctggaggaa
     6901 tatttattca ttatatagat atatttcatc atttattacc caaagttcca attgtattcc
     6961 gtgaaataat ctttggactt ggcgttggta taatcactgt tataatagcc aatctattga
     7021 attggatagt aaagcaaatt cgcaataaat aaagtactaa attctaattg atatttgtaa
     7081 tttataggag ttcactctga aaaagtaggg tttcactcaa taaaaatggc tatttaatta
     7141 tttcaataaa ctattaaata attttactgt aataaaaatt acagttatga aacaattaca
     7201 ttttatttta cccgttttat tatttattgg aatttcaata ttaccaacta catgtcatgc
     7261 tcaattcgga aaagtaatta ataaggtaat aaataaaaca gtaccaaaca aatcatctaa
     7321 tacaactaac gacggctctt cgaataaaac aaataataat tctaacaatt cggataatat
     7381 accaacaatc aaaagcaact cacctattcc tgaaaatgaa aaatacaatt gtcaagaaaa
     7441 ggaggtttct aatggctatt catcagaagt acataaaaag tatagtaata aactagtttt
     7501 ctctaaaact gaaatagtta tgagtaacca aaatgaagct actttctcca atacatttac
     7561 tttaaaagac aatatttatt caagaattta tttaagtgaa tctcctgttt atacagcaca
     7621 aactatgggt ttatgctgta aaggtggttt gtattgtatg aaattatttt atagatatat
     7681 tattgacaat aaggagacaa ataatcaata tgctgtcctt tcaacaccag aaccaaagaa
     7741 gataacttgg cagcttggaa tatctccttc aaaagaagat gttaattcag gttatccatt
     7801 aagagaaata tataaattta aagaattagc atctgaactt agcgaaggaa cgcataaaat
     7861 aagagttgag tattttatgg agattccaga tgatgatacc gaaagagggc ttttatatag
     7921 cactaaattt ggaaaagaaa tagtattagc aagtggagaa tttacgctac aagtaaaaac
     7981 ggcagataaa acagaaatga aagaaaaatt gtatgttaaa ccagcacctg taacttatag
     8041 ttctagttca tcaaatcaac aaagttgcta tgtggaagta aaaaatggta gtggagaatc
     8101 aataaaattt gatttataca caagtgcaag tcgctcaact gaaagtttat cttcaaattc
     8161 aaacaaatca attgattgta acatgtatga gaaaattgta attactgatg gtcgttgcaa
     8221 agatgttacc tannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttgataac
     8341 tttctcaaaa atttataaca ttttataatg atattcatta tcttagattg ataaactaca
     8401 agattgtgaa acttaaattc attattacac ttttaacatt cttatttttc ttaaatattg
     8461 atgctcaaaa tcctgatttc gaaattagga gaaatgctta tatagaaaat ggatttaatc
     8521 atctaaattc aaattccatc atttttcaag catataaaaa tagtccaatt gatacaacct
     8581 tattgaatga aattttaaat aatattgcat cgaataccac agcagatttt gatattgtaa
     8641 agctaattag aattttgttt ttatctaatg gtgaatatga taatctaata ttacccattc
     8701 ttgaaccgat accattttgg ctaacaaaag atgagaaatt aagagtatat tggtcagaaa
     8761 accatatgat tatgtggatg tcatctaatt ggttactaca tgaaaaatat ggaaaacaag
     8821 ttgatgatag tttgaatagc caactaaaac atttattaaa attaaaagtg gattatggtt
     8881 tttatgaatt tttttcatct gtatatctcc cttatagctt atcaggctta ttaaacttag
     8941 ctgattttgc tcaagatgta gaaataaagc atttagctac acaagctgct caaaaattat
     9001 tgaaagacat tttgttatta accaataacc aaggcgtttt ctttcctacg gcagggagaa
     9061 attattatag taaatattca actccgtata accaaaatca tagtaactta atatatcttt
     9121 taactggaat gggtgaagaa ccaacatcgg tatcacactc aggtagtttt ttagccactt
     9181 cttcaataga tgtaaattct gttatttcat catggacaag tacacttaat acgaatttta
     9241 cggttggaca ttcattatta gatggcgtta atactatcaa taaaaatatc cctttacttt
     9301 caaaagtaat ttttcaatgg agttctggag cctattttga tcctgatgtt gcacttagta
     9361 cggctactat tttaaatgaa tacaatttat ggaatcatga tgaatttaaa gattttaagg
     9421 attttaaagg tttgccatta gctatcgcac caactttagc tagaatagca agttctattt
     9481 caaaaagttc tggtatctat aatcccaaca tctctatttt taaaaacaat tcagtaacct
     9541 tatcctcagt gcaagatttt tggaaaggaa aaaatggtta tcagcaattt ccagttgttg
     9601 ctaatactgg tacgtccgct gtttttccta tttcaggcaa ggttactgaa gatttagaaa
     9661 atcgatctga actacacgct aatactcatt taccttatgt taaacaaacc gataatattg
     9721 cattagtaat gtatagacca gaaaaaggat tggctctttt tggatatact gataataaac
     9781 tagacattgc tctcaaatgg gatgcaaata cgtttgatga aataagagaa tataataatt
     9841 ggatattagg gagagaaaaa gatggttata ttgctgttag aagacattgt acggatacaa
     9901 ttctaaataa aatagcatgc gataaccatg atgggcaaac ttggattata gtagttggta
     9961 acatagaaat gtatggtagc tttgataatt ttgaaaacat aattaaaaca tcgatttttg
    10021 aaagtaaatg gtattttgac ttacctacat tacaatgggc ttatcaatct aaaattagta
    10081 tagataataa agttttagaa tacacttggt atgcagatat tttttctttt ccaactgaaa
    10141 aagaagtatc taccgcaatt attaacaaaa aagattatca agaaatatca gtttacccta
    10201 accctgctaa tacttttgta aagctatcgt ttccaaatag tataacaaaa aattcaaggt
    10261 ataaaatatt taatacagtt ggagaaactg ttttagaaaa agttatagaa ccaacagaac
    10321 tcaataattt acttatcaat acttctactt tttcaaatgg tttgtatttt ataaatatag
    10381 aaaccaaaga caaaatctat actgggaaaa ttataataca gaaataattt ctagatttca
    10441 tccatattaa ataatcaata aacatagact acaatcagta aaacttttaa tttatcacta
    10501 aattagcttc aaatacttaa aatactatat attatgtcaa aagaagtttc ctatcctaaa
    10561 gagaaaatta aaattctttt attagaaggt ttgcaccaat ctgcaacaaa tgaatttaac
    10621 aaaaaaggct atactaatat tgattcctac aaagaagcga tgacagagga agaactatta
    10681 aactgcatag aaaactatca tattattggc attcgctcta aaacaaattt aactgcaccg
    10741 gttttagaaa aagcaaaaaa actaatgacc ataggttgtt tttgtattgg cactaatcaa
    10801 gttgatttag ctaaagctac tgaattaggc attcctgttt tcaattctcc ttactctaac
    10861 actcgttcag tggcagaatt aataattgcc aactctatta tgttattgcg aggtgttcca
    10921 tctaaaagta aagcaatgca tgaaggcaat tggcaaaaaa cagctaaagc aagttatgaa
    10981 ttaagaggca aaacaattgg tataattgga tatggacata ttggaagtca agtttcagta
    11041 ttaggcgaaa gtatgggttt aaaggtgatt tattttgaca ttgaaccaaa attgccttta
    11101 ggaaatgctc aagctgtcga aaccttagaa gaattatatg caaaagcaga tattgtaacc
    11161 ttacacgtac cagcaacacc tggtacaaaa aacatgatta ctgcaaatga ttttaatcaa
    11221 atgaaagatg gtgttatttt tcaaaatcta tccagaggta atgtagttga tgtagatgca
    11281 ttaagagatg caattgttag tgggaaaatt ggtggtgctg ctgttgatgt ttttcctaaa
    11341 gaacctaaac aaaatggtcc tggattcgag tcgcctttag ttggtttaga taatgtgatt
    11401 ttaactccgc acattggtgg ctctactcaa gaagctcagg aaaatattgg aacagaagtt
    11461 ggaagtaaac tgacaagcta tatggacaat ggttcaactt taggttctaa atcagttcct
    11521 gagttaagtt tagcaccaca aaaagacaca aaacgtgttt tacattttca taaaaatgta
    11581 cctggtgtat tatctgaaat aaatacaact ttgtctaagc atcatgtaaa tattttagga
    11641 caatatttaa aaactaatga aagtattggt tatgttgttt tagatgttga taaaaatgca
    11701 gacttaaccc ttattgatga tttaaaaaca gttgccaata ctataaaagc tagaatttta
    11761 tattaaaaat tgttgctttt ttgctaaaaa aatattctta tactcgaaaa ttcttgtatt
    11821 tttaacttga atatctaatc gtatgcctta taaagaaaaa gaaatagaaa aattatatta
    11881 tagtattggc gaagttgcta gtatgttaga tgtaaataag tctttattac gattttggga
    11941 aacagaattt accaatttaa gacccaaaaa agcatctaaa ggcaatcgtt tatatacaaa
    12001 acaagatatt gattacatca aaaaaattaa aagtctagtt aaggataaag gctttacgat
    12061 tgaaggagca agaaaacaat taaaagaaaa acaagacata attatcaata acaacattta
    12121 tgtaaaaaat gattttgaca agaacgaagt gtctaaaaaa ctaaatgaaa ttaaattttt
    12181 cctttctgaa ttggctaaaa aaatataaaa tgcctaaaaa cattcaatac tttatttttt
    12241 cttttgtatt ttttatagct tgtaaatctg ataagatagt tataaaaaat ccaactgtat
    12301 tttttcaaga attaattgct gatgataaaa atgctatttt taagaatatc aatttcgatt
    12361 tttcaaaaga tgatgtaata gacgccgaaa aatcccaaca atatgatgct acagaatctt
    12421 atttagctta tagttataat ttcccaaaag atacttctaa gtttgtagaa tatgctgaca
    12481 taaaatactt ttttgatgag catgatgtct tgaaaattat tacagtaaat gtatatttga
    12541 atgacagtat tcaagaaact caattgctag ataatttcaa agaatattat tcacataaat
    12601 atgatgctcc aaaaatagat gaatatcagt attattcttg ggatgcaaca gcagatagca
    12661 aaacaacaaa aggcaaatat tattttaata taggcattag aaaactggca ggtgaatatg
    12721 gattagcttt ggagtttttg aggttataat atcatacttt actaaattaa aaatcagtat
    12781 atttctttag atttattact aaatagcaac aatcctacaa aagtcataaa tccattgata
    12841 ataagtagct ctacgccaat tttatatcca ttcaaccacg atgcattatg atttgatgag
    12901 aaatataaat acgtacaaaa tccagcacta attatacaaa ttataggcac ataaatatct
    12961 tttattcttc gagtagtaaa catcccaaag ataaataaac ccaacaaggg accataagta
    13021 attcctgcta tatccaaaac agtaaaaacc aaagattggt catcaaataa gccaaatgct
    13081 aatacaacaa atagtaataa aattgcaaat gtgatatgaa taacatatct caatttctta
    13141 tttgcatctg agcttgtttc atctttatgt aaaaaatcaa tataaaatga ggttgttaat
    13201 gtcgttagta cagaatctgc actattgaaa gttgctgccg ttagtccaat aataaatacc
    13261 aatcctgcgg cttgccctag atgattaaag gctagagtag gaaataattg atcaggtttt
    13321 tctggtaaag gaatttccaa ctgatttgca tataaataca ataaagcacc taatgccaag
    13381 aaaaatacat taacaataac aacaataata ctaaaagaat tgatgttttt ttgagcttca
    13441 tttaaagtct tacaactaag gtttttttgc atcatatttt ggtctaaacc agtcatacaa
    13501 attgcaataa aagcacctcc taaaaattgt ttccaaaaat aactttttgc tttccaatcc
    13561 cagaaaaaca cttgtgaata atcagagttt tttatttgtg caaacacttc tgctactccc
    13621 caatccaaac gttgcattat aaaaacaatg gtaaataata cacccattaa taaaaatata
    13681 gattgaaaca tatcagtcca tactaaagtt ttaataccac cttttagtgt ataaaccaac
    13741 attaaattta taatcaaagc gactgtaaca ggaaaaggaa tcccaaaacc atcaaataca
    13801 aatttttgta atactataga tgcgagaaac aacctacctc ctgctccaat tagtcgagat
    13861 aaaataaaat agaatgaacc tgttctttga gaaatggtac caatccgttt atctaaatac
    13921 gtataaatcg atgtgaggtt gtgtttatag tataatggca aaagaaaata agcaattgcc
    13981 caataaccta atacataacc taatacaact tgaaagtaat taaattgggc agcttttact
    14041 gaacctggaa cagataaata agttaatcca gaaagcgaat ctccaattaa accaaaggca
    14101 acgatatacc aaacggattc tttatttcca atgaaatatg aggcattatt tgcttttcgt
    14161 gaagtaatgt aaccaattcc aattaaacat aagaaataaa ctaaaataca tgcgagaatc
    14221 agggttggcg acataagctt tctttaatta ttagccaaaa tacaaatttt tctaactaaa
    14281 atacaaaagc aatatagtac aaactcactt ttaataagat tattttttag cagctctctt
    14341 attagcttcg tgcatttctc tgagcaaacc tcttgaaatt aaaagtttca tgatttcatt
    14401 ggttcccgca taaattcttg caactctatt atctgcatac gctctcgcaa tataatattc
    14461 ccacatatat ccataaccac catgcagctg aacgcactca tctaccacat gttgatgcat
    14521 atcggtacaa ctgtattttg ccattgaggc agtttctgct gaaagctctt tattgcaata
    14581 taattccacg catctatcta caaatgtttg gtgtatttgc agttgagttg ccaattctgc
    14641 taacttaaat tgtgtatttt gaaatgcagc aacaggctgt ttaaatgctt ctcttgatgt
    14701 ggtatataca atcgtatttt ctaatgcacc ttcagcacca ccaactgcca ttaaagctac
    14761 tgataaacgc tctcttccta atttttgcat catataaaca aagccctttc ctatttcgcc
    14821 caataaatta tcttttggta tttcaacatt atcaaaaaat aactcacagg tatcttgagc
    14881 tttcatacct acttttttga atggtttgcc ttttgaaaat cctgctctat ctgcttcaat
    14941 taaaaacaag ctaatgccta taaattcacc atcgactatt gttcttgctg caataattgc
    15001 caaatcactc atataaccat tcgtaataaa tgttttctgc ccattgacta gataatgatc
    15061 acctctatcc tcagctgtag tttttaatgc ttgcaaatca ctaccacaac ttggttctgt
    15121 cattccaatt gcagtaataa cctcgcctgt acacattttt ggcagccatt ttttcttttg
    15181 ctcttctgtt ccgtaatcta caatataagg tgcaacaata tctgaatgta aaaagaaacc
    15241 aggtccagaa tatcctgctt ttaaaatttc ctcagtgatt aaagcacaaa atgaaaaatc
    15301 taaaccagct ccaccatact gttctggaac atctaaacac aataatcctg tttcgccagc
    15361 tttcaaccaa acttcacgac ttaccatgga gtcttcttcc cattgtgcat gataaggtgc
    15421 aatttctttt tcaataaaat gttgagtagt tttttgtaat aattcgtgtt cttcggttgc
    15481 gtaaacgctt ctttttaatt tgatattttt aaacataaat ttattttttc tgaattattt
    15541 attttcttac aatattagct ccaattgctt gtaatctttt atcaataaat tgataacctc
    15601 tatctatttg gtgtacatta gaaataacac tttgtccttt tgctgaaagt gcggcaatta
    15661 acaaagcaac tccagcacga atatctggcg atgacatatt aattcctcta agttggtatt
    15721 ctctgttaat gccaataacc gttgctctgt gtggatcgca caagattatt ttagctccca
    15781 tatctatcag tttatcgaca aaaaacaagc ggctttcaaa cattttttga tgaatcaaaa
    15841 tactaccttt agcttgtgtt gcggttacca acataatact gattaaatcg ggtgtaaaaa
    15901 gtggccatgg tccatcagca attgtaagta ctgaaccatc tataaaagat tgtatttcat
    15961 aagtttcttg ttcttcaact ataatatcgt cgttttcaat ttttaattga atgcctaatt
    16021 tttcaaagta ttttggaatt aatcccaaca tatctaaacg agtattttta atacgtatcg
    16081 tagattgtgt cgtcgctgcc aaaccaataa aacttccta
//
LOCUS       DAOQCW010000052        15858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1327_length_15858_cov_5.778080, whole genome shotgun sequence.
ACCESSION   DAOQCW010000052 DAOQCW010000000
VERSION     DAOQCW010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15858
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1327_length_15858_cov_5.778080"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(235..1614)
                     /locus_tag="PLS16_06090"
     CDS             complement(235..1614)
                     /locus_tag="PLS16_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HNJ60685.1"
                     /translation="MNWNNCFSDKRFGTPSIDTDDRTEYERDWDRIVFSSAFRRLQNK
                     TQVFPLPEEIFVHNRLTHSLEVASVGRSLGKMVGELLIELEEIKKDKKAKAFYRDNLK
                     NVISAACLAHDMGNPAFGHSGEEAISKYFRKRDTDKPEDIIFKNQFSKNEWKDLITFE
                     GNANALRIITQNQKNKPRGGFKLTYSTIGAILKYPCESLASEGKKGKSHRKKYGYFKA
                     DESIFIEVCKELNMIDDSNDGFVAFKRHPFVYLVEAADDICYNIIDFEDAHRLGILDY
                     ETVRSIFMEIISMNSRIKLEHIEQRLEEYKDDRNECVAYLRANCISTLTNKCAAVFFE
                     NRDDIIKGNYEKALIDDIPEFKDIFKRLTEINIRQIYNNKKVVEIEIAGFRIMSVLVE
                     DFVTSALTPAEKREKEQQKILALLPNQFAFDENDTPYNKTMKILDFISGMTDLYALKL
                     FRKLRGIEL"
     gene            complement(1696..2493)
                     /locus_tag="PLS16_06095"
     CDS             complement(1696..2493)
                     /locus_tag="PLS16_06095"
                     /inference="COORDINATES: protein motif:HMM:NF025029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HNJ60686.1"
                     /translation="MKVNSMTLIWAICLLPLNSFNSYGSSQINENSQYNIIESDEVKF
                     PSLNEGNQSEQNNTVCLEELLEFCQEISPEALKMSLNAYQYLLKNNKLKNKEILTVVD
                     FSKPSTEERIFVVNLKSKKIISKSLCAHGQNSGDNFATSFSNTPNSLQSSLGAFIANE
                     TYIGANGLSLKLDGMENGINSNARMRSVVIHPAKYVSYDFIKENGRLGRSQGCPALPE
                     NKSKEIISLIKGGSCFFIYHPSNKYKETSAIFKSYNEQEIEQLVAQL"
     gene            complement(2698..3609)
                     /locus_tag="PLS16_06100"
     CDS             complement(2698..3609)
                     /locus_tag="PLS16_06100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNJ60687.1"
                     /translation="MTKKIGVITVTYNSKNVIEAFLDSVFNQTYTDFILYVVDNISQD
                     DTLNFVRNSQDNRVKIIQNYKNEGVAKGNNQGIQAALEDGCDLIMLLNNDVEFEYTLF
                     EKLVANLYVLNTSMIAPKMMYHPETNLIWWAGTKFKRSDGYLAPHIGIQEEDKGQYNN
                     IETMDYAPTACVLMRKEVIEDTGIMDEKFFAYFDDTDFFYRIYKQGKHKLLYFPHAQF
                     YHKVGGLSNMREGTAKKFKFNDFYIHLNTRNHIYYLRKQKLFTSYLRIVILFFRYNMR
                     FLFSGKYNVNFNTFKLLNSSYFQGLKM"
     gene            complement(3622..4875)
                     /locus_tag="PLS16_06105"
     CDS             complement(3622..4875)
                     /locus_tag="PLS16_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HNJ60688.1"
                     /translation="MITINAFKGLRPRKDNVHLVAAKPYDVLNSAEAKEEVKENPISF
                     LRISKPEINFDNGIDQYADEVYAKGNEIFNQFIQDGILIKDETPCLYIYAQTMNGRTQ
                     TGLVAGSSIDDYFNDVIKKHELTLVAKENDRIRHMKTKMAQPGMVFLTYRSVAEIDAL
                     IDNYTQNHNVEYDFVDSQDVRHQLWVVNDAIIIEQLKNLFETKVPFSYIADGHHRSAA
                     SAKVGKQLREENPNFNGTESFNYFLSCLFPSEQLQIQDYNRLVKDTNGLSTDVLLEKI
                     QENFDIEKMGSTIFKPSQLHEFSFYIDGNWYKLTAKKNTYANANAIEVLDVSILSNYL
                     LAPILNIQDQRTDKRIDFVGGIRGLGELQKRVDSGEMKLAFAFYPVSINQLMEVADSN
                     QIMPPKSTWFEPKLKSGLVINQLNG"
     gene            complement(4939..5517)
                     /gene="leuD"
                     /locus_tag="PLS16_06110"
     CDS             complement(4939..5517)
                     /gene="leuD"
                     /locus_tag="PLS16_06110"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNJ60689.1"
                     /translation="MEKFTTIISSAVPVNIEDIDTDQIIPARFLKATSREGFGENLFR
                     DWRFNSDNTEKPDFVLNNKKYQGTILVAGKNFGCGSSREHAAWAIHDYGFKVVVSSFF
                     ADIFKNNALNNGVLPVVVSPEFLKVIFTAIEADATTKLVVSLEKQTISLNGQTESFEI
                     NDYKKTCLINGYDDIDYLLNIKDKIEAFEKSR"
     gene            complement(5549..6934)
                     /gene="leuC"
                     /locus_tag="PLS16_06115"
     CDS             complement(5549..6934)
                     /gene="leuC"
                     /locus_tag="PLS16_06115"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNJ60690.1"
                     /translation="MGKTLFEKVWEKHIVQSINNGPEVVYIDRHFIHEVTTPQAFTGL
                     DNRGIKVFRPKQTVATADHNVPTLNQHLPIKEALSRHQVDTLIKNCANHGIELYGLGH
                     KYQGIVHVIGPELGITQPGMTIVCGDSHTSTHGAFGAVAFGIGTSEVEQVLATQCILQ
                     TRPKTMRINIEGNIGKGVVSKDIVLYILSKITASGGTGYFIEFAGSAIRSLSMEARMT
                     ICNMSIEMGARGGMIAPDQTTFDYVKGRTFAPQGADFDKAVEDWKLLVSDADAVFDKE
                     YTFDAADIEPMITYGTNPGMGIKISDEIPNPKGEDAGLDKSLQYMNFERGETLLQKPI
                     DYVFLGSCTNSRIEDLRMFAQLVKGKQKASNVVVWIVPGSKQVERIAKEEGIDIILKE
                     AGMELREPGCSACLGMNEDKISAGKYCISTSNRNFEGRQGPNSRTLLASPLTAAAAAI
                     TGKIVDVRTLL"
     gene            complement(7060..7839)
                     /gene="trpC"
                     /locus_tag="PLS16_06120"
     CDS             complement(7060..7839)
                     /gene="trpC"
                     /locus_tag="PLS16_06120"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HNJ60691.1"
                     /translation="MNILDKIVSRKKEEIADAKANISIRNLEQTNFFNRTTFSVSKNI
                     KAKGSSGIIAEFKRQSPSKGIINNKADVVEVVKGYENAGVVASSILTDTDFFGGNKND
                     LVQARTEVNIPLLRKDFMIDEYQFIEAKNWGADIVLLIAAILTPQEVKTFTDLAQNLG
                     LEVLLELHGEEELNHVYHKVNMVGINNRNLKTFEVDVEQSIRMAEKLGRDFIKVAESG
                     ISNIDTLKHFRTNGFEAFLIGENFMKTENPALACKDFIGQI"
     gene            complement(7842..8552)
                     /locus_tag="PLS16_06125"
     CDS             complement(7842..8552)
                     /locus_tag="PLS16_06125"
                     /inference="COORDINATES: protein motif:HMM:NF018154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HNJ60692.1"
                     /translation="MAQKFRLFAGPNGSGKSTLFKDLRQAKVIHTEIYISADSIEVTL
                     KNKKQFYFPAYRIQANQEEFIAHIKQSSLFKRIEHKEIFLKSCTIQSGILKIENTEIN
                     SYHASFVATYLVDKLFETKQSFCFETVMSHDSKIELLKKAKSLKYKIYLYFLYTNDVK
                     LNIGRVKLRAQQGEHDVPEEKIKSRYLKSLSLLPNALELAETAYVFDTSSLPATIVLQ
                     KCHNKIEKYQELPKITKL"
     gene            complement(8552..8713)
                     /locus_tag="PLS16_06130"
     CDS             complement(8552..8713)
                     /locus_tag="PLS16_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60693.1"
                     /translation="MTASIIDTEKAKMEKVLEAVSKTVVKRAKKFNQPVLILKDGKVK
                     EIFPYRKSK"
     gene            complement(8946..9926)
                     /gene="trpD"
                     /locus_tag="PLS16_06135"
     CDS             complement(8946..9926)
                     /gene="trpD"
                     /locus_tag="PLS16_06135"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNJ60694.1"
                     /translation="MKDLLHRLYFGHYLSRAEASQVVEDITNKKYSDVQNAALLSALN
                     MRLPSTEEMLGYRDTMLKQALNINIHHQKVIDIVGTGGDGKDTFNISTLACLVCAGAG
                     IKVAKHGNYSASSVSGSSNILEAVGVVFSNDETILNQQLNDANITFLHAPLFHPAMKN
                     IGALRKELGVKTIFNLLGPLSNPCEPTIQMIGVYSDVIGNLYKNVLLQLKSNFAVVHA
                     IDGYDEISLTAETRVSTKDKDIYFLPKDFGAKTIQSHEIYGGNTIESNKTIFLNVLNG
                     KGTEAQNNVVIANAALGISLYTNKNLEESLVLAKESLLGLKALACLEKLK"
     gene            complement(9931..11559)
                     /locus_tag="PLS16_06140"
     CDS             complement(9931..11559)
                     /locus_tag="PLS16_06140"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNJ60695.1"
                     /translation="MTDIDFNLNEDKMRLAVDELKNKLTKIHLGGGQKKIDNEHKKGK
                     MTARERIDYLLDKNSEFIEIAAFAGDEMYKEQGGCVSGGVVVVIGYVSGRQCMIIAND
                     ATVKAGAWFPITGKKNLRAQEIAMENKLPVIYLVDSAGAFLPMQDEIFPDKENFGRIF
                     RNNAKMSAMGIPQIAAIMGSCVAGGAYLPIMSDESLIVEGTGSIFLAGPYLVKAAIGE
                     TVEQEVLGGAFTQTAISGVCDYNMKNDQECLDTIKKLIDKYGKFETAGFDRVKSKKPK
                     ANEKEIYGIYTSATGKPYDVREVIKRMVDNSEFTEYKEEYGQTIVCAYARIDGWSVGI
                     VANQRNVVKSAKGEVQIGGVIYSDSADKAARFIMLCNQKKIPLVFLQDVTGFMVGSRS
                     EHGGIIKDGAKLVNAVSNSVVPKFTVVTGNSYGAGNYAMCGKAYDPRLIVAWPSAKIA
                     VMGGDQASKVILQIQEASIKAKGKELSEDEKTKILKEIKDRYEATTTAYYAASRIWVD
                     AVIDPLETRKWISMGIEAANNAPVEKFNVGVLQT"
     gene            11647..12639
                     /locus_tag="PLS16_06145"
     CDS             11647..12639
                     /locus_tag="PLS16_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60696.1"
                     /translation="MALQLYNPFENLYFNDNTCFLTGIDLQHSEDTITVFPEWIMEQY
                     GLANTSFTMMDNITSVLYKDLKLPCSKKVKEAFHQLDQEIKTAFEGGYESVKVLDSQR
                     LFLWMGRIVYGVLYKDMLLERAKMEKRKLELNISTRLKERMGLFHLMLQSLVAPIEFS
                     ELKPWSISIVKLKYSKDIFNYHDDTIKLIFSLGMNGFGIIACLQDNGVIKESQQDILS
                     KIGETVLHPIQFEELYARFLYATFLLQYRVNYKIEEREQALFIEALPIQEERNKSLFG
                     AKDFNMYAQVLADLWSPWGLKKSEIANFPDEPISFLEDATTFQLIRPDTIDFPF"
     gene            complement(12768..14018)
                     /gene="porV"
                     /locus_tag="PLS16_06150"
     CDS             complement(12768..14018)
                     /gene="porV"
                     /locus_tag="PLS16_06150"
                     /inference="COORDINATES: protein motif:HMM:NF033710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HNJ60697.1"
                     /translation="MLLTGNKYKINIALLVLFSFLIFQNINAQTNVTVNSAVPFLRII
                     PDARGSALGDVGIATSVDANATFYNPSKIGFSTKKVGLSLSYAPWLRSLGVTDIYVFH
                     MSSFYKFSQFDAVNMSMKFFSLGDIQYTDEVGTPLGTGSPRELALDAGYVRRLGNMFS
                     LGVNFRYIYSNLGKGQTIGSDPIKPAHAFSSDLSATFNKNFEVGSKKMKSNVMAGLNI
                     SNMGTKVAYTNSPQVKDYLPANLGIGLGYRIQPNKLHEVGIYADFNKLLVPTPKIGDK
                     DSNGVADYREKSAIGGMFGSLFDAPFKEELREWTMGIGAEYWYDHMIAVRAGYFLEAK
                     DKGNRKFFAAGVGVKYSVFGLDFSYLIPTNSQRNPLDNTFRFTLKFDFAKQADFYKED
                     VVRDKNPEVDELDKASKKKMKNGK"
     gene            complement(14074..>15858)
                     /gene="porU"
                     /locus_tag="PLS16_06155"
     CDS             complement(14074..>15858)
                     /gene="porU"
                     /locus_tag="PLS16_06155"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HNJ60698.1"
                     /translation="VGRIPADNTTKAQHFVDKVKHYYSQNAFGDWRSQLTFIADDEDN
                     NLHIDQAEIIAKTTTNQLAKYNVDKIYLDAFQQQHTINGSTYPDANLTLNNKIYNGTL
                     LVNYIGHGGGTGLAQEKLVTLTEIDNWNNENKLPIFITATCEFARFDESESFSAGERL
                     LLKEKGGAVGLLTTTRLVFANENFDINKSFMSQFTQSVNQQDLLLGDILKLAKRQTFT
                     GDGNRKFLLLGDPALRLSFPKNNVVLTSINEQTTDTLKALKKIVLKGEVRDNANQILS
                     NFNGVASIIVYDKKNTENTLANDPTSQQKSFEIQKNKIYRGKATVNNGVFQVAFIVPK
                     DINYQYGKAKISIYANSSNTDALGADTNNIIGGIDTTIIVDNKGPVVEAFMNDEQFVF
                     GGITNENPVLLLKLFDDNGINTSGNGVGHDITAILDNNTKDIKILNDFYESEIDNLQK
                     GTVKFPLDKISHGRHTLHAKAWDIMNNSGEAYTEFVVEKSAKLAISHVLNYPNPFTTK
                     TKFMFEHNKPNQNLDLKIEIFAVSGKLIKSIEQNVNTSGFRVADIEWDGRDDFGDKIG
                     RGVYIYKITLKDENGGKISQYQKLVILN"
BASE COUNT         5140 a         2827 c         2010 g         5881 t
ORIGIN      
        1 ggtcaactaa agtaaattgt tcgctttgcg gaaataatgc agtggctttg tacacattaa
       61 gtgtcaattg gtgtgctttt ttccaaacgt tcaggtcaaa ataggattga attttttctt
      121 ccataaaaaa tatttatggt ttaaaaatac caaataatta ctaaaaaatg tatgctctaa
      181 acattatagg aagattttag gcttttttat gataaataga agaaatattg ttaattacaa
      241 ttcaattcct ctcaattttc taaacaactt caaggcatat aaatctgtca ttcctgaaat
      301 aaaatccaat attttcatcg ttttattata aggtgtatcg ttttcatcaa aagcaaattg
      361 atttggcaac aatgccaata ttttttgttg ttccttttct cttttttctg ctggagttaa
      421 agccgaagta acaaaatcct ccaccaaaac gctcataatc ctaaacccag caatttcaat
      481 ttctactact tttttattat tataaatctg acgaatattt atttcagtaa gtcttttgaa
      541 aatatcttta aactcaggaa tatcgtcaat caaagctttt tcataatttc ctttaataat
      601 atcatctcta ttctcaaaaa acactgctgc acatttattg gtcaaagtac taatacagtt
      661 ggctcgtaaa taggcaacac attcatttct atcatcttta tattcttcta atctttgctc
      721 gatatgttcc agttttatcc ttgaattcat agaaatgatt tccataaaaa tagaacgtac
      781 tgtttcgtaa tctaaaattc ctaatcgatg agcatcttca aaatctataa tattataaca
      841 aatatcatct gctgcttcta ccaaataaac aaatggatgt cgcttgaaag ctacaaagcc
      901 atcattgcta tcatcaatca tatttagttc tttacaaact tcaataaata tactttcatc
      961 agctttaaaa taaccatatt tttttcggtg cgacttccct ttcttgcctt cactagctaa
     1021 agactcacat ggatatttta aaattgctcc aattgttgag taagttaatt taaaaccacc
     1081 tcgaggttta tttttttgat tttgtgtaat aattcttaaa gcatttgcat taccttcaaa
     1141 agtgattaaa tctttccatt cgttttttga aaactgattt ttaaaaataa tatcttctgg
     1201 tttatcagta tctctctttc taaaatattt agaaatagcc tcctcaccag aatgtccaaa
     1261 tgcaggattt cccatatcgt gtgctaaaca agctgcacta attacatttt ttaaattatc
     1321 tcgataaaag gctttcgctt ttttatcctt ttttatttct tctaattcaa ttaataattc
     1381 acccaccatt ttacctaaag aacggccaac acttgcaact tctaaagaat gtgtcaatcg
     1441 attatggaca aaaatttctt caggtaaagg aaaaacctgt gttttgtttt gtaatctgcg
     1501 gaatgcactt gaaaaaacaa tacgatccca atctctttca tactctgttc tatcatctgt
     1561 atcgatagaa ggtgttccaa atcgtttgtc ggaaaagcaa ttgttccaat tcattgtttt
     1621 gtcaattgat tgaatgacaa agatacaatt ctaagaatat cttttttgaa tttaatattg
     1681 tgaaacaaaa actgtttata attgagctac caactgctca atttcttgct cattataaga
     1741 tttaaagata gctgaagttt ccttatattt attacttgga tgataaataa agaaacaaga
     1801 accacctttg attaatgata taatttcttt gcttttattt tctggcaaag ctgggcatcc
     1861 ttgacttcta cctaatcttc cattctcttt tataaagtca tagctgacat attttgctgg
     1921 atgaataaca acactacgca ttctcgcatt gctatttatt ccgttttcca ttccatctaa
     1981 tttcaatgaa agtccattag caccaatata tgtttcatta gcaataaatg cacctaaact
     2041 gctttgtaaa gaattgggtg tgttagaaaa ggaagtagca aaattatcgc cactattttg
     2101 tccatgagca cacaaacttt tagaaataat ctttttagat tttagattaa caacaaaaat
     2161 tcgttcttca gttgatggtt tagaaaaatc aacaaccgtc aaaatttctt tattcttgag
     2221 tttgttattt ttcaataaat actgataagc atttaaagac atttttaaag cttctggcga
     2281 aatttcttgg caaaattcca ataactcttc taaacaaact gtattatttt gctctgattg
     2341 atttccttcg tttaaagaag ggaactttac ttcatcactt tcaataatat tgtattgact
     2401 attttcattg atttggctac tgccgtaaga gttgaatgag tttaagggta ataaacaaat
     2461 tgcccaaata agtgtcattg aatttacttt cattaaatta agtttttatg ttcgtgattt
     2521 ctattcttaa aaaccataaa acgttatcta cctatttttc ggtattcaag atacatcaaa
     2581 aatctaattc aataacaaaa actactgctg gatattctta aaaaattcct aattagcact
     2641 tttttagcaa gttattaaca aaatatttta atttgtggaa gtttaataat caatcactta
     2701 catttttaat ccctgaaagt aggaactatt taatagttta aaagtgttga aattcacatt
     2761 gtatttccca ctaaaaagga aacgcatatt gtacctaaaa aacaaaataa caattcttag
     2821 gtaagaagta aataactttt gtttgcgtaa ataatagata tgattacgag tatttaagtg
     2881 aatataaaaa tcattgaact taaatttttt ggctgttccc tctctcatat ttgacaagcc
     2941 acctacttta tgataaaatt gagcatgcgg aaaatagagc aatttatgtt ttccttgctt
     3001 atagattcta taaaaaaaat cggtatcatc aaaataagca aaaaactttt catccataat
     3061 acctgtgtct tcaattactt cctttcgcat caaaacacaa gccgttggag cgtaatccat
     3121 tgtttcgata ttattgtatt gtcctttatc ttcttcttga ataccgatat gaggtgctaa
     3181 ataaccatca cttcttttga attttgtgcc tgcccaccaa attaagttgg tttctgggtg
     3241 atacatcatt ttaggtgcta tcatacttgt attcaataca tataaattgg caactaattt
     3301 ttcaaaaagt gtatattcaa attcaacatc attgtttaac agcataatta aatcacaacc
     3361 atcctctaat gctgcttgaa taccttggtt attgcctttt gcaacgcctt cgtttttata
     3421 attctgaatt atttttactc tgttatcttg actatttctt acgaaattaa gtgtatcatc
     3481 ttgagaaata ttatctacta cataaagtat aaaatcggta tatgtctgat tgaatacact
     3541 atctaaaaat gcttcgataa cattcttgct attataagta acggtaatga cgccaatttt
     3601 ttttgtcata tacctacaaa attaaccatt tagttgatta attaccaaac ctgattttaa
     3661 ttttggttca aaccaagttg atttaggtgg cataatttga tttgaatctg caacttccat
     3721 taattgattg atagaaactg gatagaaagc gaaagctaat ttcatttctc cgctatctac
     3781 tcttttttgc aactctccta aaccacgaat gccgcctaca aaatcaatcc gtttatctgt
     3841 tctttggtct tgaatattta aaattggagc taataaataa ttactcaata tacttacgtc
     3901 taaaacttca atagcatttg cattggcata ggtatttttc tttgctgtca atttatacca
     3961 atttccatca atataaaatg aaaattcatg taattgtgaa ggtttgaaaa ttgtagaacc
     4021 cattttttct atatcaaaat tttcttggat tttttctagc aacacatcgg tacttaaacc
     4081 atttgtatct ttcactaatc tgttataatc ttggatttgt aattgctcag atggaaataa
     4141 acaagataga aaataattaa aactttctgt tccgttgaag tttggatttt cttctctcaa
     4201 ttgttttcca acttttgcag atgcagccga acgatgatgc ccatctgcga tataagaaaa
     4261 tggaacttta gtttcaaata gattttttaa ttgctcaata attattgcat cattcactac
     4321 ccaaagttga tgtctgacat cttgactatc tacaaaatca tattctacat tatgattttg
     4381 agtataatta tctatcaaag catcaatttc agcaacagaa cgataagtta aaaaaaccat
     4441 tccaggctgt gccattttgg ttttcatgtg tcttattcta tcattttctt tggctactaa
     4501 agttaattca tgctttttta taacatcatt aaaataatca tcaatagaag agccagcaac
     4561 taatcctgtt tgtgttctac cattcatcgt ttgagcataa atatacaaac aaggtgtttc
     4621 atcttttatc aaaatgccat cttgtataaa ttgattgaaa atttcattgc ctttagcata
     4681 tacctcatca gcatattggt caattccatt atcgaaatta atttcgggtt tagaaattct
     4741 caaaaatgaa atagggttct ctttaacttc ttcttttgct tcagcagaat ttaagacatc
     4801 gtaaggcttt gctgctacca aatgtacatt gtcttttctt ggtcttaatc ctttgaatgc
     4861 gttaattgtt atcataaatt attatggttt tcgtttattt ttgaagtaat ggattagctt
     4921 taaattatct aaatcaattt atctactttt ttcaaatgct tcaattttat ccttgatatt
     4981 taataaataa tcaatatcat catagccatt gattaagcat gtctttttat aatcattaat
     5041 ttcaaaactt tcagtttgtc cgtttaaact aatggtttgt ttttccaaac taactactaa
     5101 ttttgttgtc gcatcagctt caatcgctgt aaaaatcact tttaaaaact ctggcgaaac
     5161 caccacagga agcacgccat tattcaatgc attattttta aaaatatcag caaaaaaaga
     5221 agaaaccact actttaaatc cataatcatg aattgcccaa gctgcgtgtt ctctggaaga
     5281 accacagcca aaattttttc cagcaacgag tattgtgcct tgatattttt tattgtttaa
     5341 aacaaaatct ggtttttcag tattatcgga attaaaacgc caatctctga ataaattttc
     5401 accaaaacct tctctgcttg ttgctttcaa aaaacgagca ggaataattt ggtctgtatc
     5461 aatatcttca atatttacag gaactgcact tgatattatc gttgtaaatt tttccatttt
     5521 atatttttaa agtcttaatt taattttctt ataaaagcgt tctaacatca acaatttttc
     5581 cagtaattgc agcagcagca gccgttaatg gactagctaa taatgttctt gagttgggtc
     5641 cttgtcggcc ttcaaagttt cgatttgatg tagaaataca atattttcct gctgaaattt
     5701 tatcttcatt catacccaaa catgccgaac aacctggctc tcttaattcc attcctgctt
     5761 cttttaagat aatatctata ccttcttctt ttgcaattct ttctacttgt ttgctgcctg
     5821 gaacaatcca aacaaccaca tttgacgctt tttgttttcc ttttaccaac tgtgcaaaca
     5881 ttcgtaaatc ttcgattcta gaattggtac agcttcctaa aaaaacataa tctatcggct
     5941 tttgcaataa agtttctcca cgctcaaagt tcatatattg aagtgattta tccaatccag
     6001 catcttctcc ttttggattt ggaatttcat cagaaatttt aatacccatt cctggatttg
     6061 ttccataagt aatcatcggt tcgatgtcag ctgcatcgaa agtatattct ttatcaaaaa
     6121 cagcatcagc atcagaaacc aataatttcc aatcctccac tgctttatca aaatcagctc
     6181 cttgtggtgc aaatgttctc cctttaacat aatcgaaagt cgtttggtct ggagctatca
     6241 ttccacctct tgcacccatt tcaatactca tattacaaat tgtcatacgg gcttccatac
     6301 ttaaacttct aatagcagaa cctgcaaact caataaaata accagttcct ccactcgcag
     6361 taattttaga taaaatatac aatacaatat ctttagaaac cacacctttc ccaatatttc
     6421 cttcaatatt gatacgcatt gttttggggc gtgtttgcaa aatgcattgt gtcgctaaaa
     6481 cctgctccac ttctgatgtt ccaattccaa aagcaacagc accgaaagca ccatgagttg
     6541 atgtgtgaga gtcgccacaa acaatagtca ttcctggctg tgtaattcct aattctggtc
     6601 caataacgtg aacaatacct tgatatttat gacctaatcc atataattca ataccatgat
     6661 tagcacagtt ttttatcaat gtatccactt gatgtcttga caatgcctct ttaatcggta
     6721 gatgctgatt tagagttggc acattatgat cagcagttgc tactgtttgt tttggtctaa
     6781 atactttgat tcctcgatta tctaaacctg taaacgcctg tggcgttgtt acttcgtgta
     6841 taaaatgtct gtcaatataa acgacttctg gtccgttatt tatcgactgt acgatatgtt
     6901 tttcccaaac tttttcaaat aatgtcttac ccataaatct aaagaattag tgtaaaatta
     6961 ttgaaaatta gagcaataaa aggcttttaa cgattaaggt tttattaaaa tgacaatatt
     7021 gaaattttat taataaatac aaaacattaa ttgatgaatt tagatttgac ctataaaatc
     7081 tttacaagct aaagctggat tttctgtctt cataaaattc tctccaatta aaaatgcttc
     7141 aaatccattt gttctaaagt gttttagtgt atcaatatta gagataccac tttcagcaac
     7201 tttaataaaa tctctgccta atttttccgc cattcgtatg ctttgttcca catcaacttc
     7261 aaatgtttta aggtttctat tattgatgcc gaccatattt accttgtggt acacatgatt
     7321 gagttcttct tctccatgta attccaacaa aacttctaaa cccaaatttt gtgctaggtc
     7381 tgtaaaagtt tttacttctt gtggtgttaa aatcgcagca attagtaaca caatatccgc
     7441 tccccaattc ttagcttcaa taaattgata ttcatctatc ataaaatctt tacgaagcaa
     7501 tggtatatta acttccgtac gtgcttgaac taaatcattt ttgttgccac caaaaaaatc
     7561 agtatctgtt aaaatggaac tagcaacaac gccagcattt tcataacctt ttacaacttc
     7621 aacaacatct gctttattat taataattcc tttagaagga gattgtcttt taaattctgc
     7681 aataattcct gagcttcctt ttgctttaat atttttagaa acagaaaatg ttgttctatt
     7741 aaaaaaattt gtttgttcta aatttctaat agagatattt gcttttgcat cggcaatttc
     7801 ttcttttttt ctgcttacaa ttttatcgag gatattcata tttataactt tgtaattttt
     7861 ggaagttctt gatacttttc tattttatta tgacattttt gtaatacaat agtggcaggc
     7921 aatgacgaag tatcaaaaac ataagctgtt tctgcaagtt ctaaagcatt tggcaacaaa
     7981 cttaatgatt ttaagtatct acttttaatt ttttcttcag gcacatcatg ttctccttgt
     8041 tgagctctta attttaccct tccaatattt aacttaacat catttgtgta tagaaaatat
     8101 aaatagattt tgtatttcaa tgattttgct tttttcaata actcaatttt tgaatcgtgc
     8161 gacattactg tttcaaaaca aaaagattgc ttggtttcaa ataatttatc taccaaatac
     8221 gttgccacaa atgatgcatg ataagaatta atttcagtgt tttctatctt taaaattcct
     8281 gattgaattg tacagctttt caaaaatatt tctttatgtt caatacgttt aaataaagat
     8341 gattgtttga tatgtgcaat aaattcttcc tgatttgcct gtattctgta tgctggaaaa
     8401 taaaattgtt ttttgttttt caaggtaact tctatactat ctgcactaat atatatttct
     8461 gtatgaataa ctttagcttg gcgtaaatct ttaaacagcg tagattttcc agagccatta
     8521 ggacctgcaa acagtcgaaa cttttgagcc attatttact ctttctatat ggaaaaattt
     8581 cttttacctt tccgtctttt aatataagaa ctggctgatt aaattttttg gcccgcttca
     8641 ctacagtttt agatactgct tctaaaactt tttccatttt tgctttttct gtatctatta
     8701 tacttgctgt catattttaa agttaataat ttatattgaa atatgtttta attaattgtc
     8761 aattttaaac atttttattt aatacgctgc tttctaatca ttaattagta taatttttga
     8821 atcattttta ttgctcgtaa ttatcaaatc acaattagat acaattgtcc taaaatattg
     8881 cattttaaaa atccataaaa cttgaattta ttgcttttgc aactactttt tcattcataa
     8941 tagaattatt tcaatttctc taaacacgct aatgccttta gtcccaacaa actttctttt
     9001 gccaaaacca aactttcttc taaattttta tttgtataaa gtgaaatacc taatgctgca
     9061 ttggcaataa caacattatt ttgagcttct gtacctttgc catttaagac attaagaaaa
     9121 atcgttttat tactttctat cgtattgcca ccataaattt catgtgattg aatagtctta
     9181 gctccaaaat ctttaggtaa aaaataaata tctttatctt tagtcgaaac acgtgtttct
     9241 gccgttaaag aaatttcatc atatccatca atcgcatgca caacagcaaa attactttta
     9301 agttgcaata aaacattttt atacaagttt cctataacat cgctataaac accaatcatt
     9361 tgtatcgttg gttcacaagg attggaaagt ggacctaata aattaaatat tgttttaaca
     9421 cccaattctt ttctcaatgc tccaatattt ttcattgcag gatgaaataa tggtgcgtgt
     9481 aaaaaggtaa tattcgcatc gtttaattgt tgattcaaaa tagtttcatc atttgaaaaa
     9541 actacaccaa ctgcctctaa gatattagaa gaaccagaaa ctgaagaagc actgtaattt
     9601 ccatgctttg caactttaat tcctgcacct gcacagacca aacaagctaa agtagaaata
     9661 ttaaaagtat cttttccatc gccaccagtt ccaacaatat ctatcacttt ttgatgatgt
     9721 atattaatat ttaaagcttg ttttaacatc gtatcacgat agcccaacat ctcttctgtt
     9781 gatggcaaac gcatatttaa tgcagataat aatgctgcat tttgtacatc actatatttt
     9841 ttatttgtaa tatcttctac aacttgactt gcttctgctc tggataaata atgtccaaaa
     9901 tacaatctgt gtaataaatc tttcatcttc ctaggtttgt aaaacgccaa cattaaattt
     9961 ttcaactggt gcattattag ctgcttcaat gcccatagaa atccactttc tagtttctag
    10021 tgggtcaatt actgcatcta cccaaattct tgatgccgca taataagcag tagtagttgc
    10081 ttcgtatcta tcttttattt cttttaatat tttagtcttt tcatcttctg aaagttcttt
    10141 accttttgct ttgatggaag cttcctgaat ttgtaaaata acttttgatg cttggtcgcc
    10201 gcccataact gcaatttttg ctgatggcca agcaactatc aaacgagggt cataagcctt
    10261 accacacatt gcataattcc cagcaccata actatttcca gtaacaactg taaattttgg
    10321 aacaacagaa ttagaaactg cattgactaa ttttgcacca tctttaataa ttcctccatg
    10381 ctcactgcga gaaccaacca taaaacctgt aacatcttga aggaatacca acggaatttt
    10441 cttttgattg caaagcatta taaaacgagc agctttatca gcactatcag aatagattac
    10501 gcctccaatt tgcacctctc cttttgcact cttaacaaca tttctttggt tagcaacaat
    10561 acctacactc caaccatcaa ttcttgcata cgcacacaca atggtttgtc catattcttc
    10621 tttatactct gtaaactcag aattatcaac catccgcttg ataacttctc ttacatcata
    10681 aggttttcca gttgctgatg tataaatacc gtaaatttct ttttcattag ctttaggttt
    10741 tttgcttttt actctatcaa aaccagctgt ttcaaatttt ccgtatttat caatcaattt
    10801 tttaatagta tctaggcatt cttggtcgtt tttcatatta taatcacaaa caccagaaat
    10861 tgctgtttgt gtaaaagcac ctcctaaaac ttcttgttct acagtttcgc caatcgctgc
    10921 ttttactaaa tacggtccag ctagaaaaat acttccagtg ccttctacaa ttaagctttc
    10981 atcactcata ataggcaaat aggcaccgcc agcaacacaa cttcccataa ttgcagcaat
    11041 ttgaggaata cccattgccg acattttagc gttgtttcta aaaattcttc caaaattttc
    11101 tttatctgga aaaatttcat cttgcatagg taaaaaagca cccgcactat ctactaaata
    11161 gataacaggc aatttatttt ccattgcaat ttcttgagct ctcaaatttt tctttccagt
    11221 aattggaaac cacgctcctg ctttcactgt tgcatcattt gcaataatca tacattgtct
    11281 gccagaaaca taaccaataa ctaccacaac acctcctgaa acacaaccac cttgttcttt
    11341 gtacatctca tcaccagcaa aagcagcaat ctcaataaat tctgagtttt tatccaataa
    11401 ataatcaatt cgttctcgag cagtcatctt gcccttttta tgctcattat ctattttttt
    11461 ttgtccacct cctaaatgta ttttagtcaa tttattttta agctcatcaa ctgccaaacg
    11521 catcttgtct tcattcaaat taaaatctat gtcagtcatc acaacaaatt tatttttgct
    11581 tgaaaatata aaataaaacg gatttattaa aacagatacc catttttatt tgataatttt
    11641 gcaattatgg cattacaact ttacaaccca tttgaaaatt tatactttaa tgataatact
    11701 tgttttttaa caggaattga cctacaacat tctgaagata caattactgt ttttccagaa
    11761 tggataatgg aacagtatgg tttagctaat acttctttta ctatgatgga taatattacg
    11821 tctgtattgt ataaagattt aaaattacct tgttctaaga aagttaagga agcatttcat
    11881 caattagacc aagaaataaa aactgctttt gaaggtggat atgagtctgt taaagtacta
    11941 gatagtcaaa gattgttcct ttggatgggt agaatcgttt atggtgtatt atataaagat
    12001 atgctattag aaagagccaa aatggaaaaa aggaaattag aactcaatat atccactaga
    12061 ttaaaagaac gaatgggctt atttcatttg atgttacaat cattagttgc tccaattgag
    12121 tttagtgaat taaaaccatg gagcatttct attgtaaaac taaagtattc aaaagatatt
    12181 ttcaattacc atgatgatac tatcaaattg attttctcct taggaatgaa tggctttggt
    12241 attattgcat gtctgcaaga caatggtgtt attaaagaaa gtcagcaaga tattttatca
    12301 aaaattggag aaactgtttt acatccaata caatttgaag aattgtatgc tcgattttta
    12361 tatgctactt ttttattgca atatagagta aattacaaaa ttgaagagcg agaacaagct
    12421 cttttcattg aagctttacc aattcaagag gaaagaaata aatctttatt tggtgcaaaa
    12481 gattttaata tgtatgcaca agttttagca gatttgtggt cgccttgggg tttaaaaaaa
    12541 tcagaaatag ctaatttccc agacgaaccg ataagctttt tagaagatgc tactactttt
    12601 caactcattc gacctgatac aattgacttt cctttttagt ttaaagaaaa aactttaaaa
    12661 ataacttttt tctttacttg aaatgtgttc atttcatttt ggcaaacaca aagaaaacat
    12721 atctgattga aacaaacttc tctaaatcta tataaagata gatattgtta ttttccattc
    12781 ttcatttttt tcttactcgc tttatctaac tcatcgactt cagggttttt gtccctaaca
    12841 acatcttctt tatagaaatc tgcttgtttt gcaaaatcaa atttcaaagt aaatctaaat
    12901 gtattatcca aaggatttcg ttgactatta gttggtatta ggtaagaaaa atccaatcca
    12961 aatacactat attttacacc aactccagca gcaaaaaact tacgattccc tttatcttta
    13021 gcttctaaaa aataacctgc tctaactgca atcatgtggt cataccaata ctctgcacca
    13081 atacccattg tccattctcg caattcttct ttaaaaggtg catcaaacaa agaaccaaac
    13141 attccaccaa tagcagattt ctctctataa tcagcaacac cattactatc cttatctcca
    13201 atttttggtg taggcactaa taatttattg aaatcagcat aaattccaac ctcatgtaat
    13261 ttattaggtt gtattctata acctaatcca atacctaaat ttgcaggcaa ataatctttc
    13321 acctgaggac tattggtata agcaactttt gttcccatat ttgaaatatt caaacctgcc
    13381 attacattag atttcatttt tttactgcct acttcaaaat ttttattaaa ggttgcagat
    13441 aaatcagaac taaaagcatg agctggtttt attgggtccg aaccaattgt ttgtccttta
    13501 cccaaattag aatatatata tcggaagttt acacccaatg aaaacatatt tcctaatctt
    13561 cttacataac ctgcatctaa tgctaattct cttggggaac ctgtacctaa tggcgttcca
    13621 acttcatcag tatattgaat atcgcctaaa ctaaaaaact tcatagacat atttacagca
    13681 tcaaattgag agaatttgta aaaagaactc atgtgaaata cataaatatc tgttacacct
    13741 aaacttctta accaaggagc ataagaaagt gataatccaa cttttttagt agaaaaacca
    13801 atctttgatg gattgtaaaa tgtagcattg gcatcaacag aagttgcaat accaacatca
    13861 cctaaagcac ttcctcgtgc atcaggaata attctcaaaa aaggaacagc actatttaca
    13921 gttacattag tttgtgcatt aatattttga aatattagaa aggaaaatag aacaagtagg
    13981 gctatattta ttttatattt atttccagtt agtaacatac ttaatattat aaagtttatt
    14041 aaacgtaaag attgtgaaaa tagtgaaaaa aatctagttt agaataacta atttttggta
    14101 ttgtgaaatt ttccctccat tttcatcttt taatgtaatt ttatagatat aaactcctct
    14161 tccaatctta tctccaaaat catctcttcc atcccattca atatcggcaa ctctaaatcc
    14221 tgaagtattg acattttgtt ctatcgattt aattaactta ccagaaacag caaaaatttc
    14281 aatttttaaa tctagatttt gattgggttt attgtgttca aacatgaact ttgttttggt
    14341 cgtaaaagga tttggataat ttaaaacatg gcttattgct aattttgcac ttttttcaac
    14401 cacaaattcc gtataagctt ctccagaatt attcataata tcccaagctt ttgcatgtaa
    14461 agtatgtcgt ccatgactaa ttttatctaa aggaaatttc accgttcctt tttgtaaatt
    14521 atctatttct gattcataaa aatcatttaa tattttaata tcttttgtat tattatctag
    14581 tatcgcagta atatcatgcc caacaccatt gcctgatgta ttgatgccat tatcatcaaa
    14641 taactttaat aataatactg gattttcatt ggttattcct ccaaaaacaa attgttcatc
    14701 attcataaat gcttcaacaa ctgggccttt attatcaaca attattgtag tatcaattcc
    14761 tccaattata ttattggtat cggcacctaa agcatctgta ttagaactat tggcataaat
    14821 tgaaatttta gctttgccat attggtaatt aatatctttt gggactataa aagctacttg
    14881 aaaaacacca ttatttacag ttgcttttcc tctatagatt ttattttttt gaatttcaaa
    14941 tgatttttgc tgactagtgg ggtcgtttgc taaagtgttt tctgtgtttt ttttatcata
    15001 aacaataata gatgcaactc cgttaaaatt tgacaaaatt tgattggcat tatcacgtac
    15061 ttcgcctttc aatactatct ttttcaatgc ttttagtgta tctgtagttt gttcattaat
    15121 gcttgtcaat acaacattat ttttaggaaa agataaacgt aaagctgggt ctcctaataa
    15181 taaaaacttt ctattaccat ctcctgtaaa agtttgcctt tttgccaatt ttagaatatc
    15241 tccaagtaat aaatcttgtt gatttactga ttgtgtaaac tgactcatga aacttttatt
    15301 gatgtcaaaa ttttcattag caaataccaa acgtgttgtt gttaataaac caacggctcc
    15361 acctttttct tttaaaagca aacgttcgcc tgctgaaaaa ctttcacttt catcaaatct
    15421 tgcaaactca caagttgccg taataaaaat tggcaattta ttttcattat tccaattatc
    15481 aatctccgtt aaagtaacta atttttcttg tgctaagcca gttcctccgc catgtccaat
    15541 ataatttacc aacaaggttc cattataaat tttattattc agtgtcaaat ttgcatcagg
    15601 ataagtggaa ccatttatgg tatgttgctg ttgaaaagca tctaaatata ttttatctac
    15661 attatatttt gcaagttgat ttgtcgttgt tttagcaatt atttctgctt gatcaatatg
    15721 caaattatta tcttcatcat ctgcaataaa agtaagttgt gaacgccaat caccaaaagc
    15781 attttggctg tagtaatgct ttaccttatc tacaaaatgt tgtgcttttg tagtattatc
    15841 agctggaatt cttccaac
//
LOCUS       DAOQCW010000053        15714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1346_length_15714_cov_5.943419, whole genome shotgun sequence.
ACCESSION   DAOQCW010000053 DAOQCW010000000
VERSION     DAOQCW010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15714
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1346_length_15714_cov_5.943419"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="PLS16_06160"
     CDS             complement(<1..496)
                     /locus_tag="PLS16_06160"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNJ60699.1"
                     /translation="MGTEQIKSLQGGEFVIKESTTAQVFTPEDYNEEQLMVRQMASDY
                     VEKEVMPRWKEIEKQEPGLSKSLLEKAGELGLLSTAIPEQYGGMNQDVITGCIIAEEY
                     GRTGSFATTAICHIGIGTLPILYFGTEEQKAKFLPKLATGEWAASYCLTEPSSGSDAL
                     GAKTT"
     gene            complement(597..1517)
                     /locus_tag="PLS16_06165"
     CDS             complement(597..1517)
                     /locus_tag="PLS16_06165"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNJ60700.1"
                     /translation="MEKLANKHLIAIEQLSPGEIELILETAQQFKEVINRQVKKVPTL
                     RDITVANLFFENSTRTRTSFELAEKRLSADVVNFSSSTSSVKKGETLLDTVNNILSMK
                     VDMVVMRHPSPGAHQYLADRIDAAIINAGDGTHEHPTQALLDAFSLQEKLGNLQGKKI
                     AIVGDISHSRVALSNIFCLQKLGAEVTLCGPSTLIPKYIKDLGVNISHNVDETLAWCD
                     AANILRIQLERQDIQYFPSLREYAMYFGVNKQRLQKLDKQIVIMHPGPINRGVEISSD
                     VADSEHSIILEQVENGVAVRMAALYLLANK"
     gene            complement(1518..2051)
                     /gene="pyrR"
                     /locus_tag="PLS16_06170"
     CDS             complement(1518..2051)
                     /gene="pyrR"
                     /locus_tag="PLS16_06170"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNJ60701.1"
                     /translation="MSSILLHQEEINLIINRLSRQIIELNNLDSTVIIGVQPRGIYLA
                     EKVHQLIEQLANTKIPYGTIDDTFHRDDFRRGKIVKANETNINFEIEGKNVILIDDVL
                     YTGRTIRAALDALLMYGRPNKIELLVLIDRRFNRELPIQPDFVGKTIDTIEKVKVLVQ
                     WKQNEQEQDFVIFENEN"
     gene            2137..2547
                     /locus_tag="PLS16_06175"
     CDS             2137..2547
                     /locus_tag="PLS16_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60702.1"
                     /translation="MTFGLVIVLIISVCTFALGWFARGYYLRNYELNRKEFEDLKIEN
                     LELRSTIAGLKSPANDAQTLKGVIDAHIAEKESMARKIQEYETILKDYVPRKKESVKI
                     HFVEEHPTLENIQAKESSNNDVNETENDEAPNTN"
     gene            2656..4236
                     /locus_tag="PLS16_06180"
     CDS             2656..4236
                     /locus_tag="PLS16_06180"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNJ60703.1"
                     /translation="MDGFRALGLSEEVLIALEKKGFEQPTPIQEQTIPLLLNGEKDIV
                     GQAQTGTGKTAAFGLPIIERFKANKKAVQALVLAPTRELAIQVADEIKAYKGSKKIWI
                     STVYGGAPIGKQMSELEKGAHIVVGTPGRVIDLINRKKLKLDDIDFLVLDEADEMLNM
                     GFIDDIELILKSCNEDRRMLLFSATMPSRILNLAKKYMGDYDMIQIQKKEITNMSIEQ
                     SYYEVPDRDRFDALSRILDVEEDFYGIIFCNTKAETIEISNHLNEKGYSADSLHGDIQ
                     QNLREKVVKQFKSNKYGVLVATDVAARGIDISDLTHVINYGLPQDPESYVHRIGRTGR
                     AGKTGKAMSIIDPSERRKLSFIKSISKADIQKGKLPDAYEMVKIKKNRIKDEVESIIE
                     TEHFEEYLTFAQEVLSENDPKIAIAALIKHFVKDELNPKSYKVIKEPAAGKISSSKQN
                     VRLFFAKGKQDGITPKVILDMLRKDTGVDSKKINEIRIMQNFSFFNTNGEDADLILNI
                     YKKQNSRMPLVAKARERN"
     gene            4297..5085
                     /locus_tag="PLS16_06185"
     CDS             4297..5085
                     /locus_tag="PLS16_06185"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNJ60704.1"
                     /translation="MNLIEIQTATIDAVKNASSIIKEAFGKISTQDIETKSLNSLVTY
                     VDKETEQSLVAQLKNIVPEASFLTEEATIECRSSDWQWIIDPLDGTTNFIHRVPVFGI
                     SVALAYKNDIQVAVVYEPNRDECFYASKNNGAFLNGQAINVSATTQLSDSLLATGFPY
                     YDYEQTDQYLALMKKLMQNTRGIRRLGAAAIDLCYTANGIFDGFFEYSLSPWDVAAGS
                     LIVQEAGGKIYDFNGGNDYLFGRSIIATNATIYNEFYNSLKKYF"
     gene            complement(5092..5883)
                     /locus_tag="PLS16_06190"
     CDS             complement(5092..5883)
                     /locus_tag="PLS16_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60705.1"
                     /translation="MKLKFAFFSICAFYFLNNYAKVPELLTTIPEKAFDIQINYSDEI
                     IILQKGNVKKFDTQGNLLATYGNIYINQQTEIISYNTFRTILFCPDFGKIIILDKRFG
                     VVSIVDVYNIADYFVSKVGISYDNSSLWFWDDIRQVLIKTNQKNDIIYVSNNMQIYVG
                     KTIHPMMITELATKLYLNDTTQGLFVFDNTGTYKKLIPIANIQSFYEKEGLLFYVSGD
                     GVYSYNAMTFEQTNYTSVPKLNQLKIGQTVLCGINSYGLVEIWNY"
     gene            complement(5880..6659)
                     /locus_tag="PLS16_06195"
     CDS             complement(5880..6659)
                     /locus_tag="PLS16_06195"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNJ60706.1"
                     /translation="MPVSLDLKIDILDNKKLYFLSDIHLGSPNDKVSLERELKLIVFL
                     DSIQKDAHSIFFVGDIFDFWFEYKHAVPKYFIRFFAKILELQQSNIRIYFLKGNHDLW
                     MQDYISDYLNVYLVEDRIFLNCNDKLFFIAHGDGKGPGDLKYKILKKIFTNSLCKIMF
                     RWLHPDIGIKLALTWSRSSFTNPADEVFEGEDKEWLVQYAKRKMQENIKANYFIFGHR
                     HLPMQLQIAENTNFINLGDWLYHDTYAVFNGKDFELKEWKG"
     gene            6791..7363
                     /locus_tag="PLS16_06200"
     CDS             6791..7363
                     /locus_tag="PLS16_06200"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNJ60707.1"
                     /translation="MHSNQIHNDLSIKIQHKGLKKEIHLFEYQLNGAFFANFENSIIQ
                     DCNLKILINFDKRLEPYHCKIQIDGTVKTDCDKCNAAFPMTVLADFELYIKYTGEHHS
                     EEESETEIIFITRDEPEIDFTKLVYDLLHLSMPIYKVCNDVGNTAYCDKEMLKAIDQY
                     LQKTEQKNEQETEEIDPRWAKLQELKNKLN"
     gene            7385..7576
                     /gene="rpmF"
                     /locus_tag="PLS16_06205"
     CDS             7385..7576
                     /gene="rpmF"
                     /locus_tag="PLS16_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNJ60708.1"
                     /translation="MAHPKRKTSKTRRDKRRTHYKSDIPNVVTCKATGESHLSHNAYV
                     HEGDLYYNGKKLVEGYEKA"
     gene            7616..8179
                     /gene="efp"
                     /locus_tag="PLS16_06210"
     CDS             7616..8179
                     /gene="efp"
                     /locus_tag="PLS16_06210"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNJ60709.1"
                     /translation="MASTSDIRNGMCINFNSDIYQIIEFLHVKPGKGAAFVRTKLRSV
                     TTGRVLENTFPSGTKLDEVRVEHREYQYLYDEGDFSTFMNNETYEQITVDKKVINAPE
                     FLVEGMVVKIAVRAEDEMPLTCTLPDTVVLEVTYAEPGIKGDTATNTLRPSTVSSGAK
                     VMIPLFVNVGDKIKIKTETGDYMERVK"
     gene            8304..9290
                     /locus_tag="PLS16_06215"
     CDS             8304..9290
                     /locus_tag="PLS16_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60710.1"
                     /translation="MKADIKKIYDAHVAFELEQFENKLLKSNINDEVNAVWGWMGKAK
                     LNDITTSKKVKDFLERNLEDRALTEHQKEYLIDLGNSIRELALESKHTLSDFVSKDTY
                     FEIADYLIEQKEARERIISRIVKNPFYGEMLADTLYDGIKSFMAQSGPSQETVGGSIF
                     SIGKSLVGAALSGVQDNIDKNIKKFISTNLSKAIADSEDNLKEKLSTSKLKSLSRNIW
                     DKAESLKVKNLAKGVKEEHIEKIVDKGEMVAKDLIAAEAVKELAHFVIDHFFKHDGKK
                     TISKILTDNGITKEIILRESEEIAIPIIESMKKTGFLQERIEARLGKFYSTL"
     gene            9302..9700
                     /locus_tag="PLS16_06220"
     CDS             9302..9700
                     /locus_tag="PLS16_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HNJ60711.1"
                     /translation="MKKIGLLILVLLVLSQFFRPIKNNTPHTNINTIPEDVKADLKVA
                     CYDCHSNQTEYPWYSNIQPIAWWMNKHIKNGKQKLNFDDVIKQKRYKDIVETIEEKEM
                     PLKSYTIIHQDANLDVAQKERIIAWAKSKQ"
     gene            complement(9914..10909)
                     /locus_tag="PLS16_06225"
     CDS             complement(9914..10909)
                     /locus_tag="PLS16_06225"
                     /inference="COORDINATES: protein motif:HMM:NF039709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6263 family protein"
                     /protein_id="HNJ60712.1"
                     /translation="MLKKSILILIVNFSFFCHLFAGDKLDLSLNLKVGDTYKISIPMQ
                     VPTNMDISMSKESIQQLLGFQDTILSQNDSFNIDTSTIEQFSQKIKLDMQLNIVFMVK
                     VLEHNKNNYLFELYFDELSYYSKQDSTEVRFNSKSTSNKIPDKNRTEFNNLKKIIGQK
                     FLVSTNQIGKILEIQHYDKVEKLLNTKQSEESKSNNALSDQLKAKNFENTLSDIFNIL
                     PNKPVGIGDSWTKEFIIKDEVAPYIVKTKYTLKDIEEDALLINSMTIVYSEKPTLNQF
                     TTDGDGSAFIRLSKSDFFAQEQNYSLKLTMKMKLLGMDMLINSDASCVYSVKKVF"
     gene            complement(10910..12229)
                     /gene="mtaB"
                     /locus_tag="PLS16_06230"
     CDS             complement(10910..12229)
                     /gene="mtaB"
                     /locus_tag="PLS16_06230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HNJ60713.1"
                     /translation="MFSDKTIAFHTLGCKLNFAETSAITRQMEAVGFETVDFTSKADV
                     YVINTCSVTDDADAKCRNIVRRSLKNNPNAFVVVLGCYAQLKPNEIASIEGVDLVLGA
                     KDKFRLPNIITDLTKNACGQVFTSDIHEVNVFMPSFSLAERTRSFLKIQDGCDYKCSF
                     CTIPLARGKSRSDSLENILNNAKALAANGIKEIVLTGVNTGDYGKGIEDEIGLIDVVK
                     HLEQIEEIERFRISSVEPNLMTDEIIDFIANSKKFMPHFHMPLQSGSDRILGLMQRRY
                     KSALYAEKVAKIKSILPDACIGVDVIVGFPGETEEDFMETFNFIHSIDASYLHVFTYS
                     ERANTKALEMENPVPMQERHDRCIRLRNLSEKKKRHFYEQFLNSNRKVLFEDEQRGEL
                     MHGFTDNYIKVNAPYDKSLCNTIQEVSLNGFSEDGNINVNVNQSILI"
     gene            12316..13266
                     /locus_tag="PLS16_06235"
     CDS             12316..13266
                     /locus_tag="PLS16_06235"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNJ60714.1"
                     /translation="MNLAVHNKIKVGIIGVGNLGMQYINRLQHNAYYEIIGYSDVNAH
                     LNHYLEDEFALKNYDNAKRLFETCDAVIICTPNQSIMKYLHVGIKTGKHIFLGRPLIH
                     EISELLYIEDLVREAGIKMQVSLADSFSPIFKKLKEIIEEPVFVEMKRSITFETSKNN
                     TQMIQESLLKDICLLLDITKGKVQKVRYSNVFKQMDFVQIRIEFDNGSAAHISINQIS
                     NTKSFSIQAYQSSKVITANLSNRRMNIVTQRDTHLKLSETNFELEEIDTISEELNAFA
                     ESILFNKDCSNSITDFFKASELTYKILKSLEEKDKDILVS"
     gene            complement(13255..13866)
                     /locus_tag="PLS16_06240"
     CDS             complement(13255..13866)
                     /locus_tag="PLS16_06240"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNJ60715.1"
                     /translation="MNKQIYIIRHGQTDYNIKQIVQGRGVNSDLNETGRLQAQLFFEQ
                     YKTVPFDTIFTSALKRTQQTVAPFLENKIPHIIRKEIDEIAWGIFEGVQHHPSLQERY
                     YGIIESWKNGAYDNKIEGGESPNELKQRLTPFIEEIKTIEAENILVCTHGRTLRMLMC
                     LILNKPISEMDNFQHENTCLYQFEYYDNSFSLVKANDLAHLRD"
     gene            complement(13950..15548)
                     /locus_tag="PLS16_06245"
     CDS             complement(13950..15548)
                     /locus_tag="PLS16_06245"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNJ60716.1"
                     /translation="MAKFIFVTGGVTSSLGKGILAASLAKLLQARGLRVTIQKFDPYI
                     NVDPGTMNPYEHGECYVTEDGAETDLDLGHYERFLGEPTSQANNVTTGRIYLEVINRE
                     RRGDYLGKTVQIIPHITDEIKRRMLLLGNSGKYDIILTEIGGTVGDIESLPYVESMRQ
                     LKWQLPKEDYLVLHLTLVPYLASAKELKTKPTQHSVREMQSYGVQPHVLICRTEHTLG
                     EERKRKIADFCNVEPDCVIEAMDAETIYDVPINLKQEQLDRIVLEKLGYKDMPEVELG
                     KWNNFLKGHKQPSKEITVAVVGKYIELKDAYISIYESFIHAGAAHNCKVNIVALQSEE
                     IDKSNVAEKLAHVNGVFVAPGFGQRGIDGKIAVIQYARENKIPFFGVCLGMQMAVIEF
                     ARNILGIADADSTEMNTSTTNPVIDLMNDQKDIQNMGGTMRLGAYSCTIKPNTLAHKI
                     YETEEISERHRHRYEFNNQYLEQFEQAGMIASGKNKDNNLVEIIEIPEHPFFIGVQFH
                     PEYKSTILNPHSLFCAFVEAALKF"
BASE COUNT         5634 a         2483 c         2289 g         5308 t
ORIGIN      
        1 ctgttgtttt tgcacctaat gcatcagaac ctgaacttgg ctctgttaaa caataagaag
       61 cagcccactc tcctgttgct aattttggta aaaacttagc tttttgctcc tctgttccaa
      121 aatataaaat tggcaatgta ccaataccaa tatggcaaat tgcagttgtt gcaaatgaac
      181 ctgtacgacc atattcttca gcaataatac aaccagttat tacatcttgg ttcataccac
      241 catattgttc aggaatagct gttgaaagca aacctaattc accagctttt tctaataaac
      301 tctttgataa acctggctct tgtttttcta tttctttcca tcttggcatt acttctttct
      361 ctacgtaatc tgaagccatt tggcgaacca ttaattgttc ttcattgtaa tcttcaggtg
      421 taaaaacttg tgctgttgtt gattctttaa tcacaaactc tccaccttgt aatgatttaa
      481 tttgttctgt tcccatttct ttatttaatt aatcaatttt tttcgccccg aaaatacaat
      541 ttttgtttca aatttagaaa cttgtttatg aaataatgat ttcaatcatt aatctattac
      601 ttattagcta ataaatacaa agccgccata cgcacagcaa caccattttc cacttgttct
      661 aagatgatag aatgttctga atcggcaaca tctgaactaa tttctacacc tcgattaatt
      721 ggaccaggat gcatgataac aatctgcttg tctaattttt gtaagcgttg tttatttaca
      781 ccaaaataca tagcatattc tctcaaggaa ggaaaatatt gaatatcttg tctttctaat
      841 tgaatgcgta aaatattagc agcatcgcac caagctaatg tttcatcaac attatgagaa
      901 atattaacgc ctaaatcttt aatatattta ggaatcaggg ttgatgggcc acaaagcgta
      961 acttcagcac ctaatttttg caaacaaaat atattggata aagcaactct tgaatgtgaa
     1021 atatctccaa cgattgcaat tttttttcct tgtaaattgc ctaatttttc ttgaagtgaa
     1081 aatgcatcta ataatgcttg agttggatgt tcgtgtgtac catcaccagc attgataatt
     1141 gctgcatcta ttctatccgc aagatattga tgagctcctg ggcttggatg tcgcatcacc
     1201 accatatcca ctttcattga taaaatattg tttaccgtat ctaaaagtgt ttctcctttt
     1261 tttacagaag aagttgaaga tgaaaaatta acaacatcag cggataatct tttttctgcc
     1321 aattcaaatg atgttcttgt tcttgttgag ttttcaaaaa acaaatttgc gaccgtaata
     1381 tctcttaaag taggaacttt ttttacttgt ctattaataa cttctttaaa ttgttgagct
     1441 gtttcaagta ttaattcaat ttcacctgga cttagttgtt cgatagcaat taagtgttta
     1501 ttggctaatt tttccattta attttcgttt tcaaaaataa caaaatcttg ttcttgttca
     1561 ttttgcttcc attgaacaag cactttaact ttttcaattg tatcgatagt ttttccaaca
     1621 aaatctggct gaattggcaa ttctctatta aaccttctat caattaatac taacaattct
     1681 atcttatttg gtcttccgta cattaataaa gcatctaaag ctgctctaat cgttcttcca
     1741 gtatatagaa catcatcgat tagaatcaca tttttacctt caatctcaaa attaatattt
     1801 gtttcattgg ctttaacaat ttttccacgt ctaaaatcat ctcgatgaaa agtatcatca
     1861 attgtaccat acggaatttt agtatttgct aattgttcta tcagttgatg aactttctcc
     1921 gccaaataaa taccacgagg ttgtacaccg ataataactg ttgagtctaa attattaagt
     1981 tcaataattt gtctgcttaa gcggttgatg attaaattaa tttcctcttg atgcagtaaa
     2041 atagaagaca tacgatgcaa agatataatt ttataacttt ttttaataga aaaattatta
     2101 actttaggta gtcaaatttt atctaaagtt aattatatga cttttggact tgttatcgta
     2161 ttaataatca gtgtatgcac ttttgcctta ggatggtttg caagaggcta ttatctcaga
     2221 aattatgagt taaatagaaa ggaatttgaa gatttgaaga ttgaaaattt agaattaaga
     2281 agtacaattg ctgggctaaa aagtcctgca aatgatgctc aaacgctgaa aggtgttata
     2341 gatgctcaca tagctgaaaa agagtctatg gctagaaaaa tacaagaata tgaaaccatt
     2401 cttaaagatt atgttccacg aaaaaaagaa tctgtgaaaa ttcattttgt tgaagagcat
     2461 cctactttag aaaatattca agcaaaagaa tcttcaaata atgatgtaaa cgaaaccgag
     2521 aatgatgaag ctccaaatac aaattagttt acatcaattt gttaaaaaca tcaattattt
     2581 cataaattgc taacaccaaa gctttcatcg gcaaacaaat aattacgtat ctttgcaccc
     2641 taattatatt aaaatatgga cggatttaga gcgttgggtt tatctgaaga ggtattaatc
     2701 gcacttgaaa aaaaagggtt tgaacaacca acaccaatac aagaacaaac tattccttta
     2761 cttttaaacg gagagaaaga tatcgtaggg caagctcaaa ctggtactgg caaaacagca
     2821 gcatttggac ttccaattat tgaaagattt aaagcaaata aaaaagcggt acaagcttta
     2881 gtattagcac caacaagaga acttgccata caagttgcag atgaaatcaa agcctacaaa
     2941 ggttcaaaaa aaatatggat tagcacggtt tatggtggcg ctccaattgg aaaacaaatg
     3001 agtgaattag aaaaaggtgc tcatattgtt gtaggtacac ctggtcgtgt tatcgattta
     3061 atcaatagaa aaaaattaaa attagacgac atcgatttct tggttttaga tgaagcagat
     3121 gagatgttga atatgggctt tattgatgat attgaattaa ttttaaaatc atgtaatgaa
     3181 gatagacgta tgttgttatt ttcagcaact atgccttcta gaattttgaa tttggcaaaa
     3241 aaatacatgg gcgactatga tatgatacaa attcaaaaga aagagattac caatatgagt
     3301 attgagcaat cttattatga agttcccgac agagatcgtt tcgatgcctt atcaagaatt
     3361 ttagatgttg aagaagattt ttacggtatt attttctgta atacaaaagc tgaaactatt
     3421 gaaatcagca atcatttaaa cgagaaaggt tatagtgcag attcattaca tggcgatatt
     3481 cagcaaaatt taagagagaa agttgtaaag caattcaaaa gcaacaaata tggtgtttta
     3541 gtagcaactg atgtggcagc aagaggtatt gatattagtg atttaactca tgttatcaat
     3601 tatggtttac cacaagatcc tgaatcttat gtacacagaa ttggaagaac tggtcgagcc
     3661 ggaaaaactg gaaaagcaat gtctatcatt gatccatctg aaagaagaaa actatcattc
     3721 atcaagagca ttagcaaagc tgatattcaa aaaggtaaat tacctgatgc ttacgaaatg
     3781 gtaaaaatca agaaaaacag aataaaagac gaagttgaat ctatcattga aacggaacac
     3841 tttgaagaat atttaacttt tgctcaagaa gttttgagcg aaaatgatcc taaaattgca
     3901 attgctgcat taatcaagca ttttgtaaaa gatgagttaa atccaaaatc ttataaagtt
     3961 attaaagaac ctgctgctgg aaaaattagc agtagcaaac aaaatgtaag attattcttt
     4021 gcaaaaggca aacaagatgg cattacacca aaagtaattt tggatatgct tagaaaagat
     4081 acaggcgtgg atagtaaaaa aatcaatgag attagaatta tgcaaaactt ctcatttttt
     4141 aataccaatg gagaagatgc tgatttaatt ttgaatatct ataagaaaca aaatagcaga
     4201 atgcctttag ttgcaaaagc tagagaacgt aattaattta cttttccttt caatttataa
     4261 ctgaaatact tttagaaata agtgtatttt taacttatga atttaattga aatccaaaca
     4321 gcaaccattg atgccgtaaa aaatgcatct tccattatta aagaagcatt tggtaaaatt
     4381 agtactcaag atattgaaac taaaagttta aatagtttag ttacctacgt tgataaagaa
     4441 accgaacaat ccttagttgc tcaattaaaa aacattgtac cagaagcatc atttcttact
     4501 gaagaagcta ctattgaatg ccgctcaagt gattggcaat ggattattga cccactagat
     4561 ggcacaacaa acttcataca tcgagtacct gtttttggca tttcagttgc tttggcttat
     4621 aaaaatgata ttcaagtagc tgtggtttat gaacctaatc gtgatgaatg tttttatgcc
     4681 tctaaaaaca atggtgcttt tttaaatgga caagctatta atgtttcagc aactacacaa
     4741 ttaagtgata gtttgcttgc gacaggcttc ccttattatg attacgaaca aacagaccaa
     4801 tacttagctt taatgaaaaa attaatgcaa aacactagag gaattaggcg tttaggagct
     4861 gctgcaatcg atttatgtta tacggcaaat ggcatttttg atggtttttt tgaatatagt
     4921 ttatcgcctt gggatgttgc tgcgggaagc ttaattgtac aagaagctgg aggaaaaatt
     4981 tatgatttta atggtggaaa tgactatcta ttcggaagaa gtattatcgc aacaaatgct
     5041 acaatttaca atgagtttta taattcacta aaaaagtatt tttaggaaaa ttcaataatt
     5101 ccaaatctct actaaaccat aagaatttat accacaaagt acagtttgtc caattttcaa
     5161 ttgatttaat ttaggtacag atgtataatt agtttgctca aaagtcattg cattgtaact
     5221 ataaacgcca tcgcctgaaa cataaaacaa caatccttct ttttcataga aagactgaat
     5281 atttgcaatt ggaattaact ttttataagt tcctgtatta tcaaacacaa acaatccttg
     5341 cgttgtatca ttcaaataca attttgttgc taattcagta atcatcatcg gatgaattgt
     5401 ttttcctaca taaatttgca tattattgct cacataaata atatcatttt tttgattggt
     5461 ttttattaaa acttgtcgaa tatcatccca aaaccataaa cttgaattat cataagatat
     5521 gccgacttta cttacaaaat aatcagctat attatataca tcgacaatac tgaccacgcc
     5581 aaatcttttg tctaatatta taattttacc aaaatcaggg caaaacaaaa tcgtccgaaa
     5641 tgtattataa cttataattt ctgtttgttg attaatatag atattaccat aagttgccaa
     5701 taaatttcct tgcgtatcaa atttcttcac attgcctttt tgtagaatta taatttcatc
     5761 agaataattg atttgaatat caaaagcttt ctcaggaatt gttgttagca gttctggtac
     5821 ttttgcataa ttatttagaa aataaaatgc acaaatgcta aaaaaagcga atttcaattt
     5881 catcctttcc attcttttag ttcaaaatct ttaccattaa aaactgcata agtatcatga
     5941 tataaccaat cgcccaaatt gataaaatta gtattttcag caatttgaag ttgcatcggc
     6001 aaatgtcgat gaccaaaaat aaaataattt gcttttatat tttcttgcat ttttcgtttt
     6061 gcatactgta ccaaccattc tttatcctct ccttcaaaaa cttcatcagc tggatttgta
     6121 aaactgcttc tactccaagt caatgctaat tttatgccaa tatctggatg caaccaacga
     6181 aacattattt tacacaaaga attggtaaat attttcttta aaattttgta ttttaaatca
     6241 ccaggacctt tcccatctcc atgagcaata aaaaacaatt tatcattaca attcaaaaat
     6301 attctatcct ccactaaata gacatttaga taatcagaaa tataatcctg catccacaaa
     6361 tcatgattac ctttcaaaaa ataaatacga atattacttt gctgtaattc taagatttta
     6421 gcaaaaaagc gtatgaaata ttttggaact gcgtgtttgt attcaaacca aaagtcaaat
     6481 atatcaccta caaaaaagat agaatgtgcg tctttttgaa tggaatctaa aaaaacaatt
     6541 aatttcaatt ctcgctccaa agaaacctta tcatttggcg aacctaaatg aatatctgag
     6601 agaaaatata atttcttatt atctaaaata tcaattttca aatccaagga aacaggcata
     6661 tttttcaatt aatatgctat aaaaatacaa aaaaaagaca aagtctttag ataacaaatg
     6721 tttaatagct aaaaatgagg ttatttcaaa ataaatagtt atttttgcaa tccaatttaa
     6781 gttcttcgtg atgcattcta atcaaattca taatgactta tcgattaaaa tccagcacaa
     6841 agggctcaaa aaggaaatcc atttatttga atatcaatta aatggagcgt tctttgcaaa
     6901 ttttgaaaat tcaatcattc aggattgtaa tctaaagata ttaattaatt ttgataaaag
     6961 attagaaccc tatcactgta aaatccaaat tgatggaact gtaaaaacgg attgtgataa
     7021 gtgcaacgca gcttttccaa tgactgtttt ggcagatttt gaattgtata ttaaatatac
     7081 aggtgagcat cattcagaag aagaaagcga aaccgaaatc atttttatta caagagatga
     7141 acctgagatt gattttacaa aattggttta tgatttattg catttaagta tgcctattta
     7201 caaagtttgt aatgatgtag gcaatacagc atattgtgat aaagaaatgc tcaaagcaat
     7261 agaccaatat ttgcaaaaaa cagaacaaaa aaacgaacaa gaaactgaag aaattgaccc
     7321 aagatgggca aaacttcagg aacttaaaaa taaactaaat taaaaaaaac ttaaatagaa
     7381 taagatggca catcctaaaa gaaaaacatc gaaaacaaga cgtgataaaa gaagaacgca
     7441 ctacaaatca gacattccta atgtagtaac ttgcaaagca acaggcgaaa gtcatttaag
     7501 ccataacgct tatgttcacg aaggtgattt atactacaac ggaaaaaaac tagtggaagg
     7561 ctacgaaaaa gcttaattag ttttaaataa tttgtttgat ttaaaaatat aaaaaatggc
     7621 ttcaacttct gatattagaa atggtatgtg tattaatttc aactctgata tttatcagat
     7681 tatagaattt ttacacgtaa aacctggaaa aggtgctgca tttgtacgaa caaaattaag
     7741 aagtgtaaca actggaagag ttttggaaaa tacattccct tcaggtacaa aattggacga
     7801 agtaagggta gaacaccgtg aatatcaata tttatacgac gaaggcgatt tctctacttt
     7861 tatgaataat gaaacgtatg agcaaattac cgttgataaa aaagtaatta atgcaccaga
     7921 gtttttagta gaaggtatgg tggttaaaat tgcggttaga gcagaagatg aaatgccttt
     7981 gacttgtaca ttaccagata ctgttgtttt agaagtaacg tatgctgagc ctggaattaa
     8041 aggtgatact gctacaaaca cacttagacc atcaacggta tcatcaggtg caaaagtgat
     8101 gattccttta tttgtgaatg ttggcgataa aatcaaaatc aaaacagaaa ctggcgatta
     8161 tatggaaaga gtaaaataat tttcactttt ttcaaaatat acattaagca accttttagg
     8221 ttgctttttt ttattaaaaa taaatagcta aagattattc taattagttt tttttatact
     8281 atcttgatta aaattttact caaatgaaag cagatattaa aaaaatatat gatgcacatg
     8341 ttgcatttga attagaacag tttgaaaaca agctacttaa aagcaatatt aatgatgaag
     8401 ttaatgccgt ttggggctgg atgggaaaag caaaacttaa tgacatcact acgtcaaaaa
     8461 aagttaagga ttttttggaa agaaatttag aagacagagc attaacagaa catcagaaag
     8521 aatatttaat agatttaggc aattccattc gtgaacttgc attagaaagc aaacatacct
     8581 tatctgactt tgtatctaaa gatacttatt ttgaaatcgc tgattattta atagaacaaa
     8641 aagaggcaag agaacgcatt attagtagaa ttgtaaaaaa tccattttat ggcgaaatgc
     8701 ttgctgatac tttatatgat ggtataaaat cttttatggc acaaagtgga ccgagccaag
     8761 aaacagttgg tggttcaatt tttagtatcg gaaaaagttt agtaggtgct gctctttcag
     8821 gggtccaaga taatatagac aaaaatatca agaaattcat atccacaaac ctttctaaag
     8881 ccattgcaga tagtgaagat aatttgaaag aaaagctaag tacctctaaa ctaaaatcac
     8941 tttcaagaaa catttgggac aaagcagaat cgcttaaagt aaaaaattta gcaaaaggtg
     9001 taaaagaaga acatattgag aaaattgtag ataaaggcga aatggttgct aaagatttaa
     9061 ttgcagctga agctgtaaaa gaactagctc attttgttat tgaccatttc tttaaacacg
     9121 atggcaaaaa aactataagt aagatactaa cagataatgg catcacaaaa gaaataattt
     9181 taagagaatc tgaagaaatt gcaattccaa ttattgagag tatgaaaaaa acaggttttt
     9241 tacaagaacg tattgaagct agattaggca aattttattc cactttataa atatttaaaa
     9301 aatgaaaaaa atagggttat taattttagt tttattggta ctttctcaat ttttcagacc
     9361 aataaaaaac aatacaccac acacaaatat caatacaatt cctgaagatg taaaagcaga
     9421 tttgaaagta gcttgttatg attgtcatag taaccaaaca gaatatcctt ggtacagtaa
     9481 tattcaacct attgcgtggt ggatgaataa gcatatcaaa aatggtaagc aaaaattaaa
     9541 ttttgacgat gtaataaagc aaaagcgtta taaagatatt gttgaaacta ttgaagaaaa
     9601 agaaatgcct ttaaaaagct atacaattat ccatcaagat gcaaatttag atgttgcaca
     9661 aaaagaaaga attattgctt gggcaaaatc taagcagtaa tgttctatcc atagtttttc
     9721 actagtaaat tagattttta attgtccagt ttaaaccttg caactgacag gttttattac
     9781 ctgtcgccca ccctcgaaaa gcattatgta tttttgatga aattttttac tgtgagttgg
     9841 caggtgataa cacttgccaa aggcgacaaa ttagattttt aattgtctag actatgctct
     9901 tgacaattga aaatcaaaaa actttcttca cagaatacac gcatgaagca tcagaattta
     9961 taagcatatc catgcccaat aatttcattt tcatcgttaa tttcagactg taattttgtt
    10021 cttgagcaaa gaaatctgac ttactcaatc gaataaatgc acttccatct ccgtccgtcg
    10081 taaattgatt gagcgttggt ttttctgaat aaacaattgt catactatta atcaataaag
    10141 catcttcttc aatatctttt aaagtatatt ttgttttaac aatataaggt gcaacttcat
    10201 cttttataat aaattctttt gtccaagagt cgccaatacc aactggttta ttcggtagaa
    10261 tattaaaaat atcagacaag gtattttcaa agtttttagc tttcaattgg tctgaaagtg
    10321 cattattaga tttcgattct tccgattgtt ttgtattcaa taatttttca actttatcat
    10381 agtgttgaat ctctaaaatc tttccaattt ggttggtgga taccaaaaat ttttgaccta
    10441 taatcttttt taaattatta aattctgttc tatttttatc tggaatttta ttagaagtac
    10501 ttttgctatt aaatctaact tctgtactat cttgcttaga ataataagac aattcgtcaa
    10561 aatacaattc aaatagataa ttgtttttat tatgttccaa tacttttacc ataaacacaa
    10621 tattcaactg catatccaac tttattttct gagaaaattg ttcaatcgtt gaagtatcaa
    10681 tattaaaact atcattttgg cttagaatag tatcctgaaa acccaataat tgctgaatac
    10741 tttctttact catcgaaata tccatattgg taggcacttg cattggaata ctaattttgt
    10801 aagtatctcc tacttttaaa ttcaaagaca aatccaattt atctccagca aataaatgac
    10861 aaaagaaact aaaattgact atcagaataa gaatactttt ctttagcatc taaattaaaa
    10921 ttgattgatt aacattaaca ttaatatttc catcttcgct gaaaccattt agcgaaacct
    10981 cttgtatggt attacacaaa cttttatcat aaggtgcatt tactttgata taattatccg
    11041 taaaaccatg catcaattcg cctctttgct catcttcaaa taatactttt ctattgctat
    11101 ttaaaaactg ttcgtaaaaa tgtctttttt tcttttctga aagatttctt aaacgaatgc
    11161 atctatcatg tctttcttgc attggcacag gattttccat ttctaatgct tttgtatttg
    11221 ctctttcaga ataagtaaat acatgcaaat aagaagcatc tattgaatga atgaaattaa
    11281 aagtttccat aaaatcctct tctgtctcgc caggaaaccc aacaatcaca tctacaccaa
    11341 tacaagcatc tggcaaaata gattttattt tagccacttt ttcagcatat aaagctgatt
    11401 tatagcgtcg ttgcatcaag cctaaaattc tatcacttcc actttgtaaa ggcatgtgaa
    11461 aatgtggcat aaatttttta ctattggcaa taaagtcgat aatttcatct gtcatcaagt
    11521 tgggttctac agaggatatt ctaaaacgct caatttcttc aatctgttct aaatgcttta
    11581 ccacatcaat taagccaatt tcgtcctcaa tgcctttgcc ataatctccc gtatttacac
    11641 cagtcaatac aatttctttg attccatttg ctgccaaagc tttggcatta ttcaaaatat
    11701 tttctaaact atcacttcta ctttttcctc gtgctaaagg aatcgtacaa aatgaacatt
    11761 tataatcaca accatcttgg attttcaaaa aagagcgtgt tctttcagcc aaagaaaatg
    11821 aaggcataaa aacattcact tcgtgaatat ctgaagtaaa aacttggcca caagcatttt
    11881 tagttaagtc tgtaataata ttcggcaacc tgaatttatc ttttgcaccc aagaccaaat
    11941 caacaccttc aatagatgca atttcatttg gttttaattg agcataacaa cctaaaacaa
    12001 ctacaaatgc attaggatta tttttcaatg aacgtctaac aatatttcta catttggcat
    12061 ctgcatcatc agttacagaa caagtattaa taacataaac atctgccttt gaagtaaaat
    12121 caaccgtttc aaaaccaacg gcttccattt gtcttgtaat agctgaagtt tcggcaaaat
    12181 tcaatttgca acccagcgtg tgaaatgcga tagttttatc tgaaaacata gacgaagtta
    12241 agcaatatgc accaaatgtg gattaaatat aaataaaata atagttgcat tttatacttt
    12301 taatccgtta taaagatgaa cttagctgta cataataaaa taaaagttgg aattattggc
    12361 gttggtaatt taggcatgca atatatcaat cgtttacaac acaatgctta ttatgaaatt
    12421 attggctatt cagatgttaa tgcccatttg aatcattacc tagaagatga gtttgcttta
    12481 aaaaattacg ataatgctaa aagattattt gaaacgtgtg atgccgtaat aatttgtacg
    12541 cccaaccaat ccatcatgaa atatttacat gttggtatta aaactgggaa gcatattttt
    12601 ttaggtcgcc ctttgattca cgaaatatca gaacttttat atattgaaga tttagtacga
    12661 gaagctggaa taaaaatgca agtgagctta gctgatagtt ttagtccgat ttttaaaaaa
    12721 ttaaaagaaa tcattgaaga gccagttttt gtggagatga agcgaagtat cacttttgaa
    12781 acatcaaaaa ataatactca aatgatacaa gaatctttat taaaagatat ttgtttgctt
    12841 ttggatatta caaaaggaaa agttcaaaaa gttagataca gcaatgtttt taaacagatg
    12901 gattttgtcc aaattcgtat tgaatttgac aatggctctg cggctcatat ttctatcaat
    12961 caaatttcaa ataccaaaag tttttctatt caagcatatc aatcatcaaa agttatcact
    13021 gcaaatttat ccaatagaag aatgaatatt gttacacaaa gagatacgca tttaaaatta
    13081 tctgaaacca attttgagtt agaagaaata gataccataa gcgaagaatt aaatgcattt
    13141 gcagaaagta ttctctttaa taaagattgt tcaaattcta ttacagattt ctttaaagcc
    13201 agcgaattga cttataagat tttaaagagt ttggaggaaa aagataaaga tattttagtc
    13261 tcttaaatgt gctaaatcat ttgcttttac taaagaaaaa ctattatcgt aatactcaaa
    13321 ttgatacaaa caagtatttt catgttgaaa gttgtccatt tctgaaatag gcttatttaa
    13381 aattaaacac atcaacattc taagtgttct tccgtgtgta cacactaaaa tattttctgc
    13441 ttcaattgtt ttaatttctt ctatgaatgg cgttaatctt tgttttaatt catttggact
    13501 ctcaccacct tcaattttat tatcatacgc cccatttttc caactttcta taataccata
    13561 atatctttct tgtaaactag gatgatgttg aacaccttca aaaattcccc aagcaatttc
    13621 atcaatttct tttctaatta tatgtggaat tttgttttct aaaaatggtg caacagtttg
    13681 ttgtgttctt tttaaagcag aagtaaatat agtatcaaaa ggaacagttt tgtactgctc
    13741 aaaaaacaac tgtgcttgaa gacgaccagt ttcattcaaa tcagaattta caccacgacc
    13801 ctgcactatt tgcttgatat tataatctgt ttgtccgtgt cgtatgatgt atatttgttt
    13861 gttcattttt ttattagatt tttttaaagc aagagtaaaa cttaatttat ttttaattac
    13921 aattcaaata aaaattaagc attaataact caaaatttta aagctgcttc aacaaaagca
    13981 caaaaaagtg aatgcggatt gagtattgta cttttatatt ctggatgaaa ttgtacgcca
    14041 ataaaaaatg gatgttcagg aatttcaata atttctacta aattattgtc cttatttttt
    14101 ccactcgcta tcattcccgc ttgttcaaat tgctctaaat attgattatt aaattcgtaa
    14161 cgatgtctat gtctttctga aatttcttca gtttcataga ttttatgtgc caatgtatta
    14221 ggttttattg tgcaagaata agcacccaaa cgcattgtac cacccatatt ttgaatatct
    14281 ttttgatcat tcattaaatc tatcactgga tttgtggtcg atgtgttcat ctctgtcgaa
    14341 tcagcatctg caatacctaa aatatttctt gcaaattcaa ttactgccat ttgcatacct
    14401 aaacaaacac caaaaaatgg aattttattt tctctcgcat attgtattac ggcgattttt
    14461 ccatctatac ctctttgccc gaaacctggt gcaacaaaga caccatttac atgagccaat
    14521 ttctcagcaa catttgattt gtctatttct tctgactgta aggcaacaat attgactttg
    14581 caattgtgag cagctccagc atgtataaaa gattcataaa tggaaatata agcatctttt
    14641 aattcaatat atttacctac aacagctact gtaatttctt tacttggttg tttatgccct
    14701 ttcaagaaat tattccattt ccctaattcc acttcaggca tatctttata acctaatttt
    14761 tctaaaacaa ttctatctaa ttgttcttgc tttaaattaa ttggtacatc ataaatagtt
    14821 tcagcatcca ttgcctctat cacacaatct ggttcaacat tacagaaatc agcaattttt
    14881 cttttacgct cttcgcctaa agtgtgttct gttctgcaaa ttaaaacatg aggttgcaca
    14941 ccataagatt gcatttctct cacagaatgt tgagtcggtt tagttttaag ttctttggca
    15001 gaagctaaat aaggcaccaa tgttaaatgc aaaaccaagt aatcttcttt gggtaattgc
    15061 catttcaatt gtcgcatact ttcaacataa ggaagcgatt caatatcgcc aactgtaccg
    15121 ccaatttctg taaggataat atcatacttt cctgaattgc ccaagagcaa cattcttctt
    15181 ttaatctcat ctgtaatatg aggaataatt tgaacagttt ttcccaaata atcgcctctt
    15241 cgttctctat taatgacttc taaataaatt cttccagtgg taacattatt agcttgagaa
    15301 gtaggttcgc ctaaaaaacg ctcataatgt cctaaatcta aatctgtttc agcaccatct
    15361 tctgttacat aacattctcc gtgttcataa ggattcattg ttcctggatc gacgttaata
    15421 taaggatcaa acttttgaat agtaacccgc aaacctcttg cttgtagtag ctttgctaaa
    15481 gaagcagcta aaataccttt acctaaagag gaagtaacac ctcctgtaac gaaaataaat
    15541 tttgccataa ctgatgtgtg gttatggaca acaaagttag taaaataaaa ttgattaaaa
    15601 aaagaatatg ctgatttgat aatttgatga ttcaaatttt taaaacaaac atcacaattt
    15661 ttattagacc tcttgcgaaa ttcattttag acttagatta taaattcctg caat
//
LOCUS       DAOQCW010000054        15697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1352_length_15697_cov_5.558944, whole genome shotgun sequence.
ACCESSION   DAOQCW010000054 DAOQCW010000000
VERSION     DAOQCW010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15697
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1352_length_15697_cov_5.558944"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="PLS16_06250"
     CDS             complement(<1..961)
                     /locus_tag="PLS16_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HNJ60717.1"
                     /translation="MKYSLVILCFLFTNTFANSSYLNSYGFLQNIEFKLYTKDIPPQK
                     TLSNQQTTEEILMYDIIHAVWENGKWTAYTTNKTLLGVYKVGSPTIIQNSNGKKLFFI
                     ANFPGSKGGTDLYLSEFINGEWSKPRNMGKNINTPYNESNPGMLDENTLTYSTNGIIK
                     KVDINTFVIDNGGDPQFDFTAQTNNTVANAQKLAEQKLAEQKLAEQKLAEQKLAEQKL
                     AEQKLAEQKLAEQKLAEQKLAEQKLAELKLAEQKLAEQKLAEQKLAEQKLAEQKLAEQ
                     KLAEQKLAEQKLAEQKLAEQKLAEQKLAEQKLAEQKLAEQKLAE"
     gene            complement(1089..1415)
                     /locus_tag="PLS16_06255"
     CDS             complement(1089..1415)
                     /locus_tag="PLS16_06255"
                     /inference="COORDINATES: protein motif:HMM:NF013500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HNJ60718.1"
                     /translation="MTKLDRPIITDETLFLIIDGKDCPTLKNFYENIGEQLQFPDYFA
                     FNLDSFDELMNDLSWIDEPQIILLIVNFELFLGQEEKEKLKAITQILDNAILENDFIH
                     FEWFYK"
     gene            complement(1426..1884)
                     /locus_tag="PLS16_06260"
     CDS             complement(1426..1884)
                     /locus_tag="PLS16_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HNJ60719.1"
                     /translation="MKGKFSLILIIIAFIAGFFISNLTGKYKIVEKEKLEQHTNFKEQ
                     HSSSIIKNEKNDEQNNQYNDNNIPIKVYDIIKHIKKYNKAPDGYVGGRQFMNYEKLLP
                     EEDENGNKISYQEWDVNPKIVGKNRGKERLVTGSDVESILQMIITHPLMK"
     gene            complement(1894..2550)
                     /gene="fsa"
                     /locus_tag="PLS16_06265"
     CDS             complement(1894..2550)
                     /gene="fsa"
                     /locus_tag="PLS16_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HNJ60720.1"
                     /translation="MKFFIDTANLEQIKEANDLGILDGVTTNPSLMAKEGITGTENIL
                     EHYKKICSIVDGDVSAEVISTDYANIIEEGKKLAALHPNIVVKVPMIKDGIKAIKYFT
                     DNGIKTNCTLVFSAGQAILAAKAGATYLSPFVGRLDDISFDGIDLIHQIRHIYDNYNY
                     KTEILAASVRNSLHIIKCAEVGADVVTCPLSSITGLFNHPLTDKGLAQFLADYAKGNS
                     "
     gene            complement(2612..3148)
                     /locus_tag="PLS16_06270"
     CDS             complement(2612..3148)
                     /locus_tag="PLS16_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60721.1"
                     /translation="MVASTTQAQCVEDFLALRKSNIQEVNLRLSNLNWQLYDEDEQRK
                     DDIIVYTYKNENDIDAMLGIQWIDYVNRPKHYNKNRLSFQVQNKELYEQFVNEIKTLG
                     FYYIYQKNVYDNDVMLYSNSDVTIEIIESKSKYMYDGGTYYNFAFYNKLEYEVVFRDE
                     NPLENNPFTSISLYNKSK"
     gene            3322..4905
                     /locus_tag="PLS16_06275"
     CDS             3322..4905
                     /locus_tag="PLS16_06275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNJ60722.1"
                     /translation="MNKSLIPFVLALVLASGVFLGYKLQERRQGNPNIYSNTTIHSKL
                     ENILQLIDAKYVDTVNVQQLYDEAITDILGKLDPHSTYLPPIESQQEAEIMTGNFEGI
                     GIEFMISKDTIQVVVPIAGGPSEQVGLSAGDKILKINDTLVAGVKITNEDVMKKLKGV
                     KDTKVKVTILKRNNKIIDYTITRNKIPIYSVDAAFMLDAKTGFIKINRFSQTTYNEFA
                     EKLDMLLGKGMKNLVIDLRGNGGGFLDQAVKIADELLDGKKLIVYTEGRNSPKMVQNA
                     GKIGNFESGNLAILIDEGSASASEILSGAIQDWDRGTIIGRRSFGKGLVQEEIPFKDG
                     SAVRLTVAKYYTPSGRSIQKDYSHGIESYYMETSNRYAKDTTTKIEQKPKQVYHTKVK
                     NREVFGGGGITPDILVPLDTSMFNLLVLEILYNSVMNEFVYNYYQSNKANFKFKDIVD
                     FDKNFNISNEFYQSFLQYAYKNGVSSKNSIYAEKSKSYLELRLKAFLAKQQFQNDGFY
                     YVMSKDDKMIEKALKTVEK"
     gene            4909..6042
                     /locus_tag="PLS16_06280"
     CDS             4909..6042
                     /locus_tag="PLS16_06280"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HNJ60723.1"
                     /translation="MEKFAFKHIVAKTFVGLEELLEEELKALGAKNILKLKRGVQCKY
                     DKTLLYRINIESRYCLRALVSLEQFKATTSDELYQAAMQIPWEDYFSVQQTFAINSSV
                     FSTYFKHSQYASLKLKDAICDRFRKIGNNRPSINTDNPDILFNLHINEDKVTISLDSS
                     GESLHKRGYKQFQDIAPINEVLAAGLIGLSGWNREDLFIDGMCGSGTIAIEAALMAIN
                     KAPNLDRDDFCFKKWRDFDAQLLDEIIDIAHSKILDKKECVLGIDIDEKVLQKAQRNI
                     NRARLRQQITVQRKNFLKHIPPHQNGVCILNPPYDERMEQEAIDVFYKEIGDAFKQHF
                     TLYTCGIISSNLEAMKKVGLKPKRRFDMMNGKLPCKFNVYEMY"
     gene            complement(6052..6627)
                     /locus_tag="PLS16_06285"
     CDS             complement(6052..6627)
                     /locus_tag="PLS16_06285"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNJ60724.1"
                     /translation="MLVHDIIDFAKDIDTSIFIFINQKIQNAFFDFLMPIIRNKITWI
                     PIYGILIYLYFKKFKSKAIYFIALLLISLTLSDFLAHQFLKPFFARPRPCYNELINVR
                     LLIESCGGHWSFPSIHAANHFALSVGIILSRLFMKRIWIIILIFWAILIGFAQIYVGV
                     HYPFDVLAGSIYGSLLSFIIFKIMKIEKHLE"
     gene            complement(6631..7278)
                     /locus_tag="PLS16_06290"
     CDS             complement(6631..7278)
                     /locus_tag="PLS16_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNJ60725.1"
                     /translation="MKKKLVIIGAGHGISKAVAKKFGLEKYHIILIARKDEVLAEITE
                     ELSNEGISVEYYVADAKDNESIRDVFEQIWEQHQFIDSILYNVYNNRQVNIDEETADS
                     ITKDFKSNVAAAFTAIQLVLPDMEKNNTGTILFTGGGLALHPMKEFGSLSIGKAGLRS
                     LAESLHEALKPKNIFVGTVTVCGVVSENDEKYNPTKIADLFWQLQTERKQFEIQY"
     gene            complement(7290..8027)
                     /locus_tag="PLS16_06295"
     CDS             complement(7290..8027)
                     /locus_tag="PLS16_06295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ60726.1"
                     /translation="MEIDNKNWFELWFNSPFYHILYKNRDQNEANIFIDNMITKLKMD
                     YGKILDLACGKGRHAYYLAEKGFDVIGVDLAENSIKYANTMYQLPILEFYVHDMRKPF
                     RINYFNYVLNLFTSLGYFNQIQENEAVFKSSYDMLQDGGYFLVDFMNTEKVIQNLVPR
                     EHKNIDNIDFYIRREVENEKIYKHIKIEKEKNVYIYREEVQVLLPHHFHNFANNAGFT
                     VVNEFGDYQFQSFNPKTSDRYILLLKK"
     gene            complement(8031..9413)
                     /locus_tag="PLS16_06300"
     CDS             complement(8031..9413)
                     /locus_tag="PLS16_06300"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF027971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNJ60727.1"
                     /translation="MEIQFCGADKTVTGSSHLIRLKNGKQILLDCGLYQGGGKEMFDF
                     NTQWFFKPSSIDILVLSHAHIDHSGKIPKLVKDGFRGKIYCTSATRDLCAIMLLDSAK
                     IQESDAKSAQKRGNLAAKPLYDEFDVKNALKLFRTVGYDKWFYADEAIKVLFKDAGHI
                     LGSASITLDIMEGGKQTILGFTGDIGRWDRPILKDPEPMPECEFLISESTYGGITHED
                     TPQETGKLLKAITDTCIKNKGKLLIPAFSIGRTQEILYKLDQLYISGQLPEVPVYLDS
                     PLSMNATEIYKMHYECMDEDIEQFLKSDDNPFGWKHLHYITDKFESKLLNEKQEPCVI
                     ISASGMAEAGRIKHHIFHNIEDAKNTILIVGYCAKGTLGEKLVQKPEFVKIFGIEKKV
                     NAQIEIIGSMSAHADDIEIRKFLANQKLAKKIFLVHGDEKRQLRLKENLLNNGFGEIY
                     IPSIGEIVTL"
     gene            9497..9961
                     /locus_tag="PLS16_06305"
     CDS             9497..9961
                     /locus_tag="PLS16_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60728.1"
                     /translation="MFQEILKSIQDKIQAQIQEKFGLNSDQTSQSTSVLLDNFKKFFS
                     EDVMSGDFSNIKSILDNGLQSITENPTLQNFQKNVVNDLVNKVGLTDDIAQKVKDIQV
                     NELFSSLQNELLDENGKPDFSKIMSKININELQEKAKDLLGGIDLGGLFGKK"
     gene            10079..11326
                     /gene="hemA"
                     /locus_tag="PLS16_06310"
     CDS             10079..11326
                     /gene="hemA"
                     /locus_tag="PLS16_06310"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNJ60729.1"
                     /translation="MQQYKVVTITHKTTKVNRLKDYLLSDGETSDYPIQRLEQMKTNF
                     AFSELLYLNTCNRVTMFFCCDYKMSDEVLRELFLFINPSLNKELIELHVSKAMVFEGK
                     DAIRHLFGVVASLDSLVVGEREILGQIKYAYQKAKEHNLSGDSIRLAIEQAIVFAKKI
                     HTETKIGEKPISVVSLAYRSLLDWNLSNQSKVLIIGAGQTNNLMANLLIKNDFQQVTV
                     YNRTLNKAEELASRFKNAKAYSLENLPNHDEDFDVIISCTGANHIILHESTFHSINKG
                     RKKTVTIVDLAVPQDIEKAITELPFVNYIDIVSLEQKAKENLQFRKSEMFKAEAILED
                     FLNVFFELVKERKLELALSNIPNEVKEIRTVALEKAFKKDIDTLNDDAKDVLLKVMDY
                     MEQKYTALPFKASKKSLLQQINS"
     gene            11330..12913
                     /gene="hemC"
                     /locus_tag="PLS16_06315"
     CDS             11330..12913
                     /gene="hemC"
                     /locus_tag="PLS16_06315"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HNJ60730.1"
                     /translation="MSNTKVIIGTRGSKLALWQANYTQQILSKHNIEAEIKIIQTKGD
                     ASQTWNTSFDKLEGKGFFTKELEEALLNNDIDLAVHSCKDLPTVFPDGLTISAYSKRA
                     NPFDVLLINKEKVDVTLPLKLKTDAIVGTSSSRRKAQLLSYRKDITIKDIRGNVPTRI
                     SKLENGEFDAILLAQAGIDRLEIDISKFEIVPLEAPMFIPAAAQGILAYQIRENDERM
                     RQLTTCLHDENDAEISTLERKILNAFDGGCQVPLAIYVKKEADWNHVWISLAQSWNGM
                     PFRTHTQYKNLKDVDVSALVQQIRNPKPKKVFITSNLSVDSFFKTNLEAHTYQVDGIS
                     FLDFKAVDFDKSLINNADWVFLSSKHAIDFFVPQYYKKDLEHTKFAVLGKGTEMQLNK
                     YGIQADFVGDGHGDTSARLFEQKINGKVKVLFPGATQSLHQVQNNFSKGLTDITNLSV
                     YQNTMKSDICKIDADIIVCTSPMNAKAYFSKFEKESKQTVISIGRSTSSLLSEMNIEH
                     KVAYLPYDYCLVDEVLSVE"
     gene            complement(12975..13196)
                     /locus_tag="PLS16_06320"
     CDS             complement(12975..13196)
                     /locus_tag="PLS16_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HNJ60731.1"
                     /translation="MYWTLELASYLEEAPFPATKDEIIDYAIRIGAPLEVIENLQELE
                     GDEDIFESMEDIWPDYPTKEDFLFNEDEY"
     gene            13421..14167
                     /locus_tag="PLS16_06325"
     CDS             13421..14167
                     /locus_tag="PLS16_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase"
                     /protein_id="HNJ60732.1"
                     /translation="MQIKLSEIPTKAPKELEKDFIKAKTAEILNDIKKLQDILIASKK
                     NALLVILQGMDASGKDGVTKNVFGALNPLGINVAAFKKPTEEEMAHDFLWRVHKVVPS
                     KGNITIFNRSHYEDVLIQRVHHWVDEKMIKNRFEAINDFETLLEQTGTKIIKCFLHIS
                     ENEQLERLQERKTNPEKMWKHNDDDWEERKLWKQYQNAYEDVFENCSKAAKWHIIQTD
                     QNWYKEYLIADLVKNALEDMHLEYPLLNSK"
     gene            14176..15024
                     /locus_tag="PLS16_06330"
     CDS             14176..15024
                     /locus_tag="PLS16_06330"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HNJ60733.1"
                     /translation="MLYNQLLDNGFVIINLLNVDEVSYFNELCQKYLKTDQQDFISGS
                     HFLSKEENAFINNELHKTIKSKVQVLFPDLELLGGTLATKKKGKSVLKAHNDWDIVNE
                     KSYNSYNLWISLVDTDKYNGTLGLVPNSHLWQHGHRGFGIAGQFEQFTNDFVNLGIEP
                     ELKAGQAILYNHKLIHFSRPNQKDEARNVAIIGMKDKAASLQASLSTDGQFIETYAVE
                     EADFYGFNIENIKQKPLISKEPVVTQKLTLSQIKKLYQQHLSEAYKSTNQSSSSFIDK
                     IKAWFQ"
     gene            complement(15033..>15697)
                     /locus_tag="PLS16_06335"
     CDS             complement(15033..>15697)
                     /locus_tag="PLS16_06335"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNJ60734.1"
                     /translation="NNGLLLQSKKFLTHLREMCTQYGILLIFDEVISGFRVGFEGAAG
                     LYEIQPDIITFGKIIGGGMPVGAYAASKEIMSNVSPDGPVYQAGTLSGNPVAMAAGIA
                     QLKQCLTPYFYNILQQKTDVLVDGINKHIENKGYDCHLFNIGSIFWIAFSKEKHINSA
                     DKIDSNKMTLFSKLHKYLLENGIYIGPSGYEVGFVSSAHSMEDINRTIEVFSKGLDVA
                     FA"
BASE COUNT         5517 a         2244 c         2364 g         5572 t
ORIGIN      
        1 gttcagccaa cttctgttca gctaattttt gttctgctaa tttttgttct gctaattttt
       61 gttctgctaa tttttgttca gccaactttt gttcagctaa cttctgttca gctaacttct
      121 gttcagctaa tttttgttca gctaatttct gttcagctaa tttctgttca gctaatttct
      181 gttcagctaa tttttgttca gctaattttt gttcagctag tttcagttca gctaattttt
      241 gttctgctag tttctgttca gctaatttct gttctgctag tttctgttct gctagtttct
      301 gttctgctag tttctgttct gctagttttt gttctgctag tttttgttct gctagtttct
      361 gttcagctag tttctgctca gctaatttct gagcatttgc tactgtatta tttgtttgtg
      421 cagtaaaatc aaattgtgga tcaccaccat tatcgattac aaatgtattg atatcgactt
      481 tttttattat tccattagtt gaataggtaa gtgtattttc atctaacatt cctggattgg
      541 attcattata tggtgtattt atatttttgc ccatatttct tggtttagac cattctccat
      601 ttataaattc agatagatat aaatcagtac cgccttttga accagggaaa tttgctataa
      661 agaataattt tttaccatta ctattttgga taattgttgg actcccaact ttataaacgc
      721 ccaaaagtgt tttgttggtc gtataggcag tccatttgcc gttttcccaa actgcatgaa
      781 taatatcata cattaaaatt tcttcagtcg tttgttggtt tgataatgtt ttttgaggtg
      841 ggatatcttt tgtatatagt ttaaattcaa tattttgaag aaatccataa ctatttaaat
      901 agctactatt tgcaaaagta ttggtaaata aaaaacataa gattactaat gagtatttca
      961 tatcaactta aaaattaaat aattaattac gaaaaataaa taaattcata ttaaaatgat
     1021 tcttttgttt tccacatctg tataaaaaca tcgattttgt ttacattgtc tagtgattgt
     1081 ggttgcaatt atttataaaa ccattcaaaa tgaataaaat cattttctaa tattgcatta
     1141 tctaaaattt gggtaatagc ctttagtttt tctttttctt cttggcctaa aaataattca
     1201 aaattgacga ttaaaagtat gatttgaggt tcatctatcc acgaaaggtc attcattaat
     1261 tcatcgaaac tgtctaaatt aaatgcaaaa taatcaggga attgtaattg ctcaccaatg
     1321 ttttcataaa aattctttag tgttgggcaa tcttttccat caataattag aaacagtgtt
     1381 tcatctgtaa ttattggtct atctaatttt gtcattcgaa aagttttatt tcattaaagg
     1441 atgcgtaatg atcatttgta aaatagactc gacatcagaa cccgtaacta gtctttcttt
     1501 acctctattt ttgccaacaa tttttggatt gacatcccat tcttggtatg aaattttatt
     1561 gccgttctca tcttcttcag gtaataattt ttcataattc ataaactgtc tgccacctac
     1621 ataaccatca ggtgctttgt tgtacttttt gatatgctta ataatatcat atacttttat
     1681 aggaatatta ttgtcattat attgattatt ttgctcatca tttttctcat ttttgataat
     1741 agaagaagaa tgttgttctt tgaaatttgt atgctgttct aatttttctt tctctacaat
     1801 cttatacttt ccagtaagat tcgaaatgaa aaagccagca ataaatgcaa ttataattaa
     1861 tataagacta aactttcctt tcataaagta gtattatgaa tttcctttag cataatctgc
     1921 taaaaactgt gccaatcctt tgtcagttaa tggatgatta aacaaaccag tgatggaaga
     1981 aagtgggcat gtaacaacat ctgctccaac ttcagcacat tttattatat gtaatgaatt
     2041 tctaacagaa gctgctaaaa tttcagtttt gtaattgtag ttatcataga tatgtctaat
     2101 ttggtggatt aaatcaatac catcaaatga aatatcatct aaacgtccta caaatgggct
     2161 taaataagtt gctccagctt ttgcagccag aattgcttga ccagcagaaa atactaatgt
     2221 gcaatttgtt ttaatgccat tgtccgtaaa gtattttatc gcttttattc catcttttat
     2281 catgggtact tttaccacaa tatttggatg taaagcagct aattttttac cttcttcaat
     2341 aatgttcgca taatctgtag aaataacttc tgcactcaca tcaccatcta caatactgca
     2401 tattttttta taatgctcta agatgttttc tgttcctgta attccttctt tagccatcaa
     2461 agacgggtta gtggtaacac cgtctaagat gcctaaatca tttgcttctt taatttgctc
     2521 taaattcgct gtatcaatga aaaatttcat aacttataat tgtattttaa ttgataacaa
     2581 atataagcga atgaaacgag aaaatgaatt tttattttga tttattgtac aacgaaatac
     2641 ttgtaaatgg attattctct aatggatttt catctctaaa aacaacttca tattctaact
     2701 tattatagaa cgcaaaattg taataagtgc caccatcata catatattta cttttagatt
     2761 ctatgatttc aattgttaca tcactattag aatataacat cacatcgttg tcatagacat
     2821 tcttttggta aatgtaatag aaaccaagag ttttaatttc atttacaaat tgttcataca
     2881 attctttgtt ttgaacttga aatgataatc tgtttttgtt gtaatgtttt ggacgattta
     2941 catagtcaat ccattggata cctaacatcg catcgatgtc attttcattt ttgtaagtat
     3001 aaacgatgat atcgtcttta cgttgctcat cttcatcata caactgccaa ttcagattgg
     3061 ataatcttaa attaacttct tgtatgttac tttttcttaa tgctaagaag tcttctacgc
     3121 attgtgcttg ggttgtgctt gctaccatta ttactaataa tagtaacaaa aaagtgtttt
     3181 ttatcatgtt gggtattttt atgttaatgc aatattaaat aaaatttatg aatatgtcaa
     3241 gtctttttga aaattatttt taaaaaaaat tattaaagtt attttataaa taggacactt
     3301 atatatcctt acatttgtaa catgaataaa tcgctcattc cttttgtgct tgctttagtt
     3361 ttagcatcag gtgttttttt aggatataag ttacaagaaa gacgacaagg taatccaaat
     3421 atttattcca atacaactat acattctaag ttggaaaata ttttacaatt aatagatgct
     3481 aaatatgtag ataccgtaaa tgttcaacaa ttatatgatg aagcgattac agatatactt
     3541 ggtaaattag atccacattc tacttattta cctccaatcg aaagccaaca agaagctgaa
     3601 attatgactg gaaactttga agggattggt attgaattta tgatttcaaa agataccatt
     3661 caagtagttg ttccaattgc tggtggacct tcagaacaag ttggtttaag tgctggcgat
     3721 aaaattttaa aaatcaatga tactttagtt gcaggcgtaa aaattactaa tgaagatgta
     3781 atgaaaaaat taaaaggtgt gaaagacact aaagtaaaag ttactatttt aaaacgcaat
     3841 aataaaataa tcgattacac aattactcgt aataaaattc caatttattc agtagatgca
     3901 gcatttatgt tagatgcaaa aacaggattt ataaaaatta atcgttttag tcaaacaacg
     3961 tataatgaat ttgcagaaaa gttagatatg ttattaggta aagggatgaa aaatttagtg
     4021 attgatttac gtggaaatgg tggtggtttt ttagaccaag cagttaaaat tgcagatgaa
     4081 ttattagatg gcaagaaatt aattgtttat acagaaggaa gaaattcgcc taaaatggtt
     4141 cagaatgctg gaaaaattgg caattttgaa tcaggaaact tagctatttt aattgatgaa
     4201 ggttctgctt ctgccagtga aattttgagt ggtgctattc aagattggga tagaggtaca
     4261 atcatcggaa gacgttcctt tgggaaaggt ttagtgcagg aagaaattcc ttttaaagat
     4321 ggctctgcgg tacgtttgac ggtagcaaaa tattatacac caagtggtag aagtattcag
     4381 aaagattatt cacatggaat tgaatcttat tatatggaaa cctcaaatag gtatgccaaa
     4441 gataccacaa ctaaaataga gcaaaaacca aaacaagtgt atcatactaa agtaaaaaat
     4501 agagaggttt ttggtggtgg tggcattacg cctgatattc tagttccttt agatacaagc
     4561 atgtttaatc tattggtttt agaaatattg tataattcag taatgaatga gtttgtttat
     4621 aattattatc aaagcaataa agctaatttt aaatttaaag atattgtaga ttttgataaa
     4681 aactttaata ttagtaatga attttatcaa tcatttcttc aatatgccta taaaaatgga
     4741 gtaagttcaa aaaatagtat ttatgctgaa aaatcaaaat cttatttaga attaagattg
     4801 aaagcatttt tagctaaaca acaatttcaa aatgacggtt tttattatgt tatgtctaaa
     4861 gatgataaaa tgattgaaaa agcattaaag actgttgaaa aataaggtat ggaaaagttt
     4921 gcatttaaac atattgtggc taaaactttt gtgggtttag aagaattatt agaagaagaa
     4981 ttaaaagctt taggtgctaa aaatatttta aaactaaaac gtggtgtgca atgtaaatac
     5041 gataaaacat tattgtatag aattaatata gaaagtcggt attgtcttag agcattagta
     5101 agtttagagc aatttaaagc aacaacttcc gatgaattgt atcaggcagc aatgcaaatt
     5161 ccttgggaag attattttag tgtgcaacaa acttttgcaa ttaatagtag tgtattttca
     5221 acctatttta aacattcgca atatgcttct ttaaaactaa aagatgctat ttgtgataga
     5281 tttagaaaaa tcggcaataa tagacccagt atcaatacag acaatcctga tatcttattt
     5341 aatttgcata taaatgaaga taaagttacg attagtttgg atagttcagg agagagtttg
     5401 cacaaaagag gctataagca atttcaagat atagcaccaa tcaatgaagt attggcagcg
     5461 ggtttaattg gactgtcagg ttggaatagg gaagacctat ttattgatgg aatgtgtgga
     5521 agtggaacga ttgcgattga agctgcactt atggcaatta ataaggcacc taatttagat
     5581 agagatgatt tttgttttaa aaaatggcga gatttcgatg cccaattatt agatgaaatt
     5641 attgatatag cacattcaaa aattttagat aaaaaagaat gtgtgcttgg tattgatata
     5701 gatgaaaaag tgttacagaa agctcaacgt aatatcaatc gtgcgaggtt aagacaacaa
     5761 attacggtac aacgaaagaa ttttttaaaa catattccac cacatcaaaa tggagtttgt
     5821 atcctaaatc ctccgtacga tgaacgtatg gaacaagagg caattgatgt gttttataaa
     5881 gaaattggag atgccttcaa acaacatttt actttatata cttgtggcat tatttcttct
     5941 aatttagaag cgatgaaaaa ggtaggttta aaacccaaaa gacgctttga tatgatgaat
     6001 ggaaaattgc cttgtaagtt caatgtttat gaaatgtatt agtttttagg tttattctaa
     6061 atgcttttca attttcatga ttttgaatat tataaaactt agcaaactac cataaatact
     6121 tcctgctaga acatcgaatg gataatgaac gccaacataa atttgtgcaa acccaataag
     6181 tatagcccaa aatattaata taattatcca aattctcttc ataaaaagtc tgcttaaaat
     6241 aattccaacg gacaaagcaa agtgattagc agcatgaatg gaaggaaaac tccaatgtcc
     6301 accgcaactt tcaatcaaca atctgacatt tattaattca ttataacaag gacgaggtct
     6361 agcaaaaaat ggttttaaaa attgatgagc taaaaaatca ctaagtgtta aacttatcaa
     6421 aagtaaagca atgaaataga ttgctttgct tttgaatttt ttaaaatata gatagattaa
     6481 aataccataa attggaatcc aagtaatctt gtttctaatt ataggcatta aaaaatcaaa
     6541 aaatgcattt tgtatttttt gattaataaa gataaatatg gaagtgtcaa tatctttagc
     6601 aaagtctatg atgtcatgaa caagcataga ttaatattga atctcaaatt gctttctttc
     6661 agtttgtaat tgccaaaata aatcagcaat ttttgtagga ttatatttct catcattttc
     6721 actaacaact ccacaaacag taactgttcc aacaaaaata tttttaggtt ttaatgcttc
     6781 gtgtaaagat tcagccaaac ttctcaatcc tgcttttcct atcgaaagtg aaccaaattc
     6841 cttcatagga tgaagtgcta aaccaccgcc agtaaataaa attgtacctg tattgttttt
     6901 ttccatatca ggtaatacta attgaatggc agtaaatgct gctgcaacat tggatttaaa
     6961 atctttggta atactatctg cagtttcttc atcaatattt acttgtctat tattgtaaac
     7021 attatataaa atgctatcta taaattgatg ttgctcccaa atttgttcaa aaacatcacg
     7081 tattgattca ttgtcttttg catctgcaac ataatattct acactgatac cttcattaga
     7141 aagctcctca gtaatttctg ctaatacttc atcttttctt gcaattaata tgatatgata
     7201 cttttctaaa ccaaattttt tagcaactgc tttactaatt ccatgtcctg ctccaataat
     7261 tactaacttt tttttcatat ataaattttc tattttttca ataaaaggat atacctatct
     7321 gatgttttgg gattgaaaga ttgaaattga taatctccaa attcgtttac aacggtaaaa
     7381 cctgcattat ttgcaaagtt gtgaaaatga tgtggtaata aaacttgaac ttcctctcga
     7441 taaatatata catttttttc tttttctatt ttgatgtgtt tgtatatctt ttcattttct
     7501 acttcacgac gtatataaaa atcaatatta tcaatgtttt tatgttctcg tggcacaaga
     7561 ttttgaatta ctttttcagt attcataaaa tcaactagaa aataaccgcc atcttgtaac
     7621 atatcataag aacttttaaa aacagcttca ttctcttgaa tttgattgaa ataacctaaa
     7681 cttgtaaata aattcaatac ataattgaaa taattaattc taaaaggctt tctcatatca
     7741 tgtacataaa actctaaaat cggtaattga tacatcgtat tggcatattt tatactgttt
     7801 tctgccaaat ctacaccaat aacatcaaat cctttttcag ctaaataata agcatgtctt
     7861 ccttttccac aagctaaatc taatatttta ccataatcca tttttaattt ggtaatcata
     7921 ttgtctatga aaatattagc ttcattttgg tctctatttt tatataaaat atgataaaaa
     7981 ggagagttaa accataattc aaaccaattt ttattatcta tttccatttt ttataaagtt
     8041 acaatttcac caattgatgg gatataaatc tcaccaaatc cattatttaa taagttttct
     8101 tttaatctaa gttgtctttt ttcatcacca tgaaccaaaa aaatcttttt ggctaatttt
     8161 tgattcgcta aaaattttct aatttcaata tcatcagcat gagcactcat actaccaatg
     8221 atttcaattt gagcatttac tttcttttct attccaaata tttttacaaa ttcaggcttt
     8281 tgaactagtt tttcgcccaa tgtacctttt gcacaataac caactattaa aatagtattt
     8341 ttggcatctt ctatattgtg aaaaatatga tgtttgatac gtccagcctc agccattcct
     8401 gatgcactaa tgataacaca aggttcttgt ttttcgttta ataattttga ttcaaattta
     8461 tctgtaatat agtgtaaatg tttccaccca aatgggttat catcactttt tagaaactgc
     8521 tcaatgtcct cgtccataca ttcataatgc attttataaa tttcagtagc attcatagaa
     8581 agcggactat ctaaataaac tggaacttca ggtaattgtc ccgaaatata gagttgatct
     8641 aatttatata aaatctcttg agtgcgtcca atactaaaag caggtatcaa taattttcct
     8701 ttattcttaa tgcaggtatc tgtaatggct tttaataatt ttcctgtttc ctgaggcgta
     8761 tcttcgtgtg taataccacc ataagtagat tctgaaatta gaaattcaca ttcaggcatt
     8821 ggctctgggt ctttgagtat tggtctgtcc catctgccta tgtcgccagt gaaaccaagt
     8881 atagtttgtt tgccaccttc cataatatct aatgtaatag aagcactgcc tagaatatgt
     8941 ccagcatctt taaataatac ttttatagct tcatcagcat aaaaccattt gtcatatccc
     9001 acagtacgga ataatttcaa agcatttttt acatcaaatt cgtcatataa aggctttgca
     9061 gccaaattac ctcttttttg tgcagatttt gcatcacttt cttggatttt tgcactatct
     9121 aataacatga ttgcacataa atcacgtgtt gcagatgtac aataaatttt tccacgaaaa
     9181 ccatctttta ctaattttgg aatttttcct gagtggtcaa tatgtgcatg acttaatact
     9241 aaaatatcta ttgatgatgg cttaaaaaac cattgtgtat taaaatcaaa catttctttg
     9301 ccaccacctt ggtataaacc acagtctagc aaaatttgtt tgccgttttt tagacgtatt
     9361 aaatgcgaac ttccagttac tgttttatca gcaccacaaa attgtatctc cataatttaa
     9421 ttataacaaa actatgtaat atgatttaga ttttataatt tgcatgtata aattaattca
     9481 ttaaaattta ttttttatgt ttcaagaaat tctaaaaagt attcaagaca aaattcaagc
     9541 acaaattcaa gagaaatttg gtttaaatag tgatcaaact tcgcaaagca cttcagtttt
     9601 gttagacaat tttaagaagt ttttttctga agatgttatg tcaggtgatt tttctaatat
     9661 aaaatcgatt ttagataatg gcttacaaag tattactgag aatcctacat tacaaaattt
     9721 ccaaaaaaat gtagtgaatg atttggttaa taaagtaggt ttaacagatg atattgctca
     9781 aaaggtaaaa gatattcagg taaatgaatt gtttagttct ctacagaatg aattattaga
     9841 cgaaaatgga aagcctgatt ttagcaagat aatgagtaaa atcaatatca atgaattaca
     9901 agaaaaagct aaagatttat taggtggcat cgatttaggt ggactttttg gaaaaaaata
     9961 attttgtgga taagatgtta gtcatacttt aagtttatat tacctaactt taggtatgat
    10021 atatatcatt tttatttcat taaaaaatga tgcatctttg taaaagaaac gaaaaaacgt
    10081 gcagcaatat aaagttgtaa ccataacaca caaaacaacc aaggtaaacc gcctaaaaga
    10141 ctatttgctt tcagatggtg aaacttcaga ctatccaatc caaaggttgg agcagatgaa
    10201 gacaaacttt gcgttttctg aattactata tttgaatact tgcaacagag ttaccatgtt
    10261 tttctgttgt gattataaaa tgagcgatga agtattgaga gaattattct tatttatcaa
    10321 tccatcatta aataaagaat taatagaatt acacgtttca aaagcaatgg tttttgaagg
    10381 taaagatgct atcaggcatt tatttggtgt agtagcttca ttagactcat tggtagttgg
    10441 cgaaagagaa attttaggac aaattaaata tgcttatcaa aaagcaaaag aacataattt
    10501 gtctggcgat agcattcgtt tagcgattga acaagccatc gtttttgcaa aaaaaatcca
    10561 tacggaaact aaaattggtg aaaaaccaat ttcagtggtt tctttagcat atcgttcttt
    10621 attggattgg aacttatcta atcaatctaa agtattgata ataggagctg ggcaaaccaa
    10681 taacttgatg gcaaatttat taattaagaa tgattttcag caagttacag tatataatag
    10741 aactttaaat aaagcagaag aattagcctc aagatttaaa aatgccaaag cttatagctt
    10801 agaaaattta cccaatcatg atgaagattt tgatgtaatt atttcttgta cgggtgccaa
    10861 tcatattatt ttgcatgaat ccacattcca tagcattaat aaagggcgaa aaaaaacagt
    10921 tactattgta gatttggcgg tacctcaaga tattgagaaa gctattactg aattgccttt
    10981 tgtaaactat attgatattg tttctttaga acaaaaagct aaagaaaatt tacaatttag
    11041 aaagagtgaa atgtttaagg cagaagctat tttagaagat tttctgaatg ttttctttga
    11101 attagtaaaa gaaagaaaat tggaattggc attgtcaaat attccgaacg aagtaaaaga
    11161 aattagaact gttgctttgg aaaaagcatt taaaaaagat attgatacat tgaatgatga
    11221 cgcaaaagat gtattattga aagtaatgga ctatatggaa caaaaatata ctgcattacc
    11281 atttaaagca tctaaaaaaa gtttgcttca acaaattaat tcctaaaaat tgagcaatac
    11341 aaaagttatt atcggtacac gtggtagtaa attagcattg tggcaagcta actataccca
    11401 acaaattcta tctaagcata atattgaagc tgaaattaag attattcaaa caaaaggcga
    11461 tgcttcacaa acttggaata caagttttga taaattggaa ggaaaaggtt tttttaccaa
    11521 agaattagaa gaagcattgt tgaataatga tatagattta gccgtacatt cttgcaaaga
    11581 tttacctact gtttttcctg atggactaac catttcagct tattccaaaa gagccaatcc
    11641 ttttgatgtt ttattgatta ataaagaaaa agtagatgtt acacttcctt tgaaattgaa
    11701 aacagatgca attgttggta cttcaagttc aagacgtaaa gctcagttgc tttcatatag
    11761 aaaagatatt actataaaag atattcgtgg aaatgtgcca acgagaattt ctaaattgga
    11821 aaatggagaa tttgatgcaa ttttattagc acaagcagga attgaccgat tagaaataga
    11881 tatttctaag tttgaaattg ttccattaga agctccaatg tttattcctg cagcagcaca
    11941 aggtatttta gcatatcaga ttcgtgaaaa tgatgaacga atgcgacaat tgaccacttg
    12001 cttgcatgat gaaaacgatg cagaaatttc aactttagaa agaaaaattt tgaatgcttt
    12061 cgatggtggt tgtcaagttc ctttggcaat ttatgtcaaa aaagaagcag attggaatca
    12121 tgtttggatt agtttggctc aaagttggaa tggaatgcct tttagaacac atacgcagta
    12181 taaaaaccta aaagatgttg atgtttcggc attggtacag caaatcagaa atcctaaacc
    12241 taagaaagtt tttataacca gtaatttatc agttgatagt ttttttaaga caaatttaga
    12301 agcccatact tatcaagtag atggtatttc ttttttagat tttaaagcag ttgatttcga
    12361 taagtctttg ataaataatg ctgattgggt tttcttatca agtaagcatg caatagattt
    12421 ctttgttcct caatattata aaaaggattt agagcatact aaatttgcag tattaggcaa
    12481 aggaacagaa atgcaattga ataaatatgg aatccaagca gattttgtag gagatggaca
    12541 tggcgataca agtgctagat tatttgaaca aaaaataaat ggcaaagtaa aagttttgtt
    12601 tcctggtgca actcaatctt tacatcaagt acaaaataat tttagcaaag gtttaacaga
    12661 tattaccaat ctatctgtgt atcagaatac tatgaagtca gatatttgta aaattgatgc
    12721 tgatattatt gtatgcacaa gcccgatgaa tgccaaggct tatttttcta aatttgaaaa
    12781 agaatccaag cagactgtca tttcaattgg acgttcaact tcttcattat tatcagaaat
    12841 gaatatagaa cataaagttg cgtacctacc ttatgattat tgtttagttg atgaggtgtt
    12901 gagtgttgag tgattttaca aataaaaata ggctgcaaag gcagcctata ctatatttct
    12961 aagaaaaaac tagtttagta ttcatcttcg ttaaataaga aatcttcctt ggttggataa
    13021 tctggccaaa tgtcttccat tgattcaaaa atatcttcat caccctctaa ttcttgtaag
    13081 ttttcaataa cctctaaagg tgcacctatt ctaatagcat agtcaataat ctcatctttt
    13141 gtagccggaa atggagcttc ttctaaatac gacgctaatt ctaatgtcca atacataatt
    13201 ttgccttttt tttaatgatt aacaaatatt gttccgtttt tgattttcgc acaaatatag
    13261 acttaaattt taattataaa aattttattt tcaatcggta ttttttagtg tttataagtc
    13321 gtttttattg agttacaata taagttagca tatataatat ttattcaaaa aatttacttc
    13381 aatcaattaa tttagttatt ttaggactac aatattcatt atgcaaatta agttatcaga
    13441 gataccgact aaagcaccaa aagaattaga aaaagatttt ataaaagcta aaactgctga
    13501 aatccttaac gatattaaga aattacaaga tattttaatt gcttctaaga aaaatgcttt
    13561 attagtgatt ttgcaaggta tggatgcaag tggaaaagat ggtgttacta aaaatgtttt
    13621 tggagcatta aatccattag gtatcaatgt tgcagctttt aaaaaaccaa cagaggagga
    13681 gatggctcat gattttttgt ggagagtgca taaagttgtg ccatcaaaag gaaatattac
    13741 tattttcaat cgttcacatt atgaagatgt tttaattcag cgagtgcatc attgggtaga
    13801 tgaaaagatg ataaaaaata gatttgaagc tatcaatgat tttgaaaccc tattggaaca
    13861 aactggaaca aaaattatta aatgtttttt gcatatttct gaaaatgaac aattggaacg
    13921 tttgcaagaa cgtaaaacta atcctgaaaa aatgtggaaa cacaatgatg acgattggga
    13981 agaacgaaaa ttatggaaac aatatcaaaa tgcttatgaa gatgtttttg aaaattgctc
    14041 gaaagctgca aaatggcata ttattcaaac agatcaaaat tggtataaag aatatttaat
    14101 tgctgattta gtgaaaaatg ctttggaaga tatgcattta gaataccctt tattaaactc
    14161 caaataattg tttctatgtt gtacaaccaa cttttagata atggatttgt aattattaat
    14221 cttttgaatg ttgatgaagt aagctatttt aatgaattgt gtcaaaagta tttaaaaaca
    14281 gaccaacaag attttatttc tggttctcat tttttatcca aagaggaaaa tgcttttatc
    14341 aataatgaat tacacaaaac catcaaatcc aaagtacaag ttttgtttcc agacttagaa
    14401 ttattaggtg gcactctagc aactaaaaag aaaggaaaat ctgtactcaa agcccataat
    14461 gattgggata ttgttaatga aaagtcctat aattcctaca atttatggat aagtttagta
    14521 gatactgata aatataatgg aactttagga ttagttccaa attcgcatct ttggcaacat
    14581 ggtcatcgtg gttttggaat tgctggacaa tttgaacaat ttacgaatga ttttgtaaat
    14641 ttaggcattg agccagaatt gaaagctggt caagccattt tgtataatca taaattgata
    14701 catttttcaa gacctaatca aaaagatgaa gctagaaatg ttgcaattat aggcatgaaa
    14761 gacaaagctg cttctttgca agcttcttta agcactgatg gacaattcat tgaaacttat
    14821 gctgttgaag aagcagactt ttatggtttt aatattgaaa atatcaaaca aaagcctttg
    14881 atttcaaaag agccagtagt aactcaaaaa ttaacactta gccaaattaa aaaattatac
    14941 caacaacatt taagtgaagc atataaatca acaaatcaat cttcatcgtc ttttatcgat
    15001 aaaataaaag cgtggtttca ataagcttac ttttaagcaa atgcaacatc taagccttta
    15061 gagaaaactt caatagttct attaatatct tccattgaat gagctgagga tacaaaacct
    15121 acttcataac cagatggacc gatataaata ccattttcta gtaaatattt atgtaattta
    15181 ctaaatagag tcatcttatt gctatcaatt ttatccgcac tattaatatg cttttctttt
    15241 gaaaatgcaa tccaaaaaat agaaccaata ttaaataaat gacagtcgta acccttattt
    15301 tcaatatgtt tattgatgcc atcaaccaat acatctgttt tttgttgtaa tatattatag
    15361 aaataaggtg ttaagcattg ttttagttga gcaattcctg ctgccattgc tactggattt
    15421 ccacttaaag taccagcttg ataaacaggt ccatcaggcg aaacattact cataatttct
    15481 ttagatgcag cataggcacc aacaggcatt ccacctccaa taattttgcc aaatgtgata
    15541 atatctggct gaatttcata caagccagca gcaccttcaa aacctactct aaagccagaa
    15601 ataacttcgt caaaaatcaa taaaatgcca tattgagtac acatttctcg taaatgtgtc
    15661 aagaattttt tactttgtaa taacaatcca ttatttg
//
LOCUS       DAOQCW010000055        15654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1358_length_15654_cov_6.025450, whole genome shotgun sequence.
ACCESSION   DAOQCW010000055 DAOQCW010000000
VERSION     DAOQCW010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15654
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1358_length_15654_cov_6.025450"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..640
                     /locus_tag="PLS16_06340"
     CDS             161..640
                     /locus_tag="PLS16_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60735.1"
                     /translation="MPIPDIEQYTIDTVMTPTDTSGYIYGRIYENKSEQGRIFEAYTA
                     TSYLNGDTILSISTTYKNQKLYILFNSNQLGKYTASPDVSNPMSCFILFDSNVSNLTD
                     LLVNLRVGEIKLNSFDSVNNIFRGKINVSNIGFGTNTYDIRGDILLKINLTSFPTTP"
     gene            complement(630..1316)
                     /locus_tag="PLS16_06345"
     CDS             complement(630..1316)
                     /locus_tag="PLS16_06345"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNJ60736.1"
                     /translation="MALEVLYEDNHIIIVNKKSGDIVQADITQDEPLTEPVKRYLKEK
                     YNKPGEVFLGVVHRIDRPVSGVVVFARTSKALSRMTNLFRTRDVKKTYWAVVKNKPEV
                     NTATLIHYHQKDEARKRAKLFNKEVAHSKICELEYKYLTSSDKYHLLEVYPTTGRFHQ
                     IRAQLSKIGSPIKGDLKYGFERPNENASIHLHARSIEFIHPISMKEISIIAPVPKDDV
                     IWQYFEKVKA"
     gene            1336..2187
                     /locus_tag="PLS16_06350"
     CDS             1336..2187
                     /locus_tag="PLS16_06350"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNJ60737.1"
                     /translation="MNKQPVVILGMHRSGTTMITKILENLGLYVGEEKEENHEALFFW
                     EINNWIFDLHTARPELPHNMQFTNPKTKQIIEESLQYFVQSPRKKKYLGSLNTNYQSL
                     QALDFNFGWKDPKNTFTLEFWKSIFPNPKIIHVYRNPIDCISSYIERDLVMKNQFQWD
                     WKKKVKRRFLITHKFNYNFRLYNLEQGYQLWQEYVQKAIDLQKDNSNYLAIQYEQFLK
                     QPEIEIQKMLAFCNLSPSEQVIQQQINTIQKDRAYAFVSNEKYVDFYQTIKNDKLIGQ
                     LDYQNIV"
     gene            2191..3129
                     /locus_tag="PLS16_06355"
     CDS             2191..3129
                     /locus_tag="PLS16_06355"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNJ60738.1"
                     /translation="MQEKVDILLATYNGERFLQKQIDSILQQTYSNFTIYIRDDGSKD
                     GTIKIIKDYAQKYPNKIIFIEDILGNLGVTQNFNELMKYSSANYIAFSDQDDIWLPNK
                     IEQSLKVLADLENKHQGTPAFVYSDMQIINEEDQFTYPSFWKLASLSPKYFTFNRLLM
                     QSIPHGCTILMNKRLKEIAFPIPQNAILHDHWLSLVTVNFGITMPILEPLILLRNHGA
                     NLTQKKTNNFKRLQRFYKNFGTNDEYLKHLNLRIEQGKSFYNEYFNQLSVENQHILEQ
                     FLLLETTSGWKRKMIYIKNKFYRTTFFHTLKMIWRA"
     gene            3147..3722
                     /locus_tag="PLS16_06360"
     CDS             3147..3722
                     /locus_tag="PLS16_06360"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HNJ60739.1"
                     /translation="MENIKKCDWCLGKPLYEKYHDEEWGVPVFDDQKQFEFLVLESAQ
                     AGLSWYTILQRREGYRKAFVNFDYKKIAKFSSEKIEELMQFEGIIRNRKKIESAINNA
                     QRFMEIQKEFGSFSNYIWRFVDGKSIVNQFKTLKDVPATTPISDALAKDMKKRGFKFL
                     GSTILYAHMQATGLVNDHLLDCHRHQQCKIK"
     gene            3723..4808
                     /locus_tag="PLS16_06365"
     CDS             3723..4808
                     /locus_tag="PLS16_06365"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNJ60740.1"
                     /translation="MQIPFFDIRRQYHAIKQEIDEAVLVCLENGQFIGGDEVKKFEHN
                     LAEYLQINHVIACGNGTDALQLALMSLGLPIGSKIIIPAFTYIAPIEVAKFLGYEIIF
                     ADVDKQTFNINLEHIKKVYTDDVRAVVVVHLFGQICSDIDEINQFCLDKNIFLIEDNA
                     QSISAEKIINRNSLMTTSFFPTKNLGAFGDGGAVLTNDEILSKKIRQIASHGQTKKYE
                     HTIIGINSRLDTLQAAILNIKLQYLDRNIEKRKEIANLYNKAFASIPQIETPKDFGRH
                     TFHQYTLKVDEEHRDNLKEYLKQNGIETNIYYPIPAHLQSAYLQSISLPNTEMLCKTV
                     LSLPIFPELKPNEQLYIIKNILDYFQK"
     assembly_gap    4923..5022
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5023..5535
                     /gene="purQ"
                     /locus_tag="PLS16_06370"
     CDS             <5023..5535
                     /gene="purQ"
                     /locus_tag="PLS16_06370"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HNJ60741.1"
                     /translation="IAKQSPIMKSVAKHAADGGIVWGICNGFQILCEAGLLPGALLMN
                     DHQKFVCQNQYIKPQTLNSLITRNLDLNKAYNVPIAHGEGRYFADEATLNEMTVNDQI
                     LFHYCDENGNINEQSNPNGSTRNIAGICNKKRNVFGMMPHPERASEKVLGNTDGLALF
                     ESLLLLQTAL"
     gene            5572..7587
                     /locus_tag="PLS16_06375"
     CDS             5572..7587
                     /locus_tag="PLS16_06375"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNJ60742.1"
                     /translation="MKRILQYTNFLILLFFSLYLNAQGMQSHLSFKTSVETLGNNEYN
                     LKITCTLDKDWHVFSQFTDDGGPLPTRFIFKNNKDIQLIGGVKEVGKLHKKFDELFGV
                     NVSSFDNQVTFVQKVKVSNPKAILEGEFDGQVCKDEEGCMPFGPEKLNFRFDNTEEAK
                     TNDTISSSLVQVDTSKSEDTTALRSDKFNWQHADNGCSVQSEKKDKSMLLIFLFGFIG
                     GLVALLTPCVFPMVPLTVSFFTKGGKDKKQGIKKALTYGLSIIVIYVLLGLVITSLFG
                     SDALNAMSTNVWFNMAFFVIFVVFAFSFFGFYEITLPSSWANQSDSLASKGGNLGIFF
                     MAFTLALVSFSCTGPIIGTLLVEAATGGGPTILGRIPIKPTIGMLGFSTALALPFTLF
                     ALFPQWLQSLPKSGGWMSTVKVTLGFIELALALKFLSIADMTQNWGILRIEPFLIIWI
                     ILFIVLAMYLLGVFQYFKKETKEHISIPRKLFGLASLGFAIYMAYGLFTYQPLKLLSG
                     LAPPVGYNFKNEKNETFIHFKDYDEGLAYAKTHNLPILLDFTGFGCVNCRKVEEHVWT
                     NPTIDKLLHKYVIISLYVDDRKELPQDQWYESDATGKLREVKTIGQKWSDFQALHFKT
                     NAQPYYVLIDKEERILNQPVDYSFSSSVANYKNFLECGLNMEKQLNH"
     gene            7702..8787
                     /locus_tag="PLS16_06380"
     CDS             7702..8787
                     /locus_tag="PLS16_06380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNJ60743.1"
                     /translation="MPTRFCKKYIFLVIFVFVGMMKGYAQDPSFAQFLNFRQYLNPAL
                     TGSERGLNVSMIYKNQWFYVPGGFNTYGIAADVQSSRLSSGIGIIAYRDVEAKALTRN
                     YVGASYAYIVRLSKNANLHFGIRAAFVNNGLDRTKLLFSDQIDPEIGASGGTPSITTI
                     PNRTVNYFDLDAGTVMRFRFDINDRPVHNSIGFAVSHLTRPDESFLNIESKIPMRFTL
                     HYGSMFPIYSKKMYNRDPMFYISPVFKFDYQSKLRVYNAGFYTTFKPIYTGIMYQLNK
                     YRFDNTHGLIILGGIDWDLGYDEMMTLNIGYSYQIDFSGIGTKSRGQHEITLRMNFND
                     VAIKNPKKKAKAIDCYSFPGRKAVKIF"
     gene            8828..10795
                     /gene="dnaG"
                     /locus_tag="PLS16_06385"
     CDS             8828..10795
                     /gene="dnaG"
                     /locus_tag="PLS16_06385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNJ60744.1"
                     /translation="MKTTIFFNFTRVITQDTIRKIIDTAQALDVVADFVTLKKSGSIY
                     KGNCPFHQERTPSFVVNPNKNIFKCFGCGKGGDAIGFIMEYEKLSYPEALRYLAQKYN
                     IEVEETHTQSEESKEEQSQIESLYIALNFAAKFYQQQLTESEDGRNIALSYCKERGFS
                     NETITTFQIGYSPDSFDAFYKEAKSNAFNEDILLKAGLIKEKNEKRYDFFRDRLMFPI
                     WNVSGKVIAFGGRILKKIDNQPKYINTAETDVYHKSQILYGIFQAKSDIRKNDACFLV
                     EGYTDVISLHQSGIKNVVASSGTALTKEQVFLIKRFTENIIILYDGDAAGIKAALRGL
                     DIVLELGLNVKIVLLPDGEDPDSFVKQKGKEEALQFIKENQQDFILFKASQSISDVKN
                     DPIQKAEAIKDIVRSITLIQDNIKRQLYIKECATIVDIDEKTLINEVNKIKIQQIKKR
                     ENVPEEDVEQLASLQEKYIEPQKKESQIPEFFYQERDIIRVLLEHADKSMNEEDNVGA
                     YILSELVEVPFKNTIFDKIIKHYINAYEQDIPFDTISDATKIEDNQIKLAIIDIQSSK
                     YEISENWLKKHEIIITEANRTYKTDVASVLNRYKYLKFMELLKDLDEKIKLADTENKM
                     EELYSSLQKKIKLIGVRNELAKEIGTVTHPY"
     gene            11039..12397
                     /gene="rseP"
                     /locus_tag="PLS16_06390"
     CDS             11039..12397
                     /gene="rseP"
                     /locus_tag="PLS16_06390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNJ60745.1"
                     /translation="MNLIQVFYLFLGLSLLIILHEFGHYLPAKLFKTRVRKFYLFFDF
                     LFPFSKVLPFSIFKKKIGDTEYGLGWFPFGGYVDIAGMVDETTSAEDLEQDDTPKENQ
                     FRFKPAWQRLIIMVGGVTVNMVLAMVIYAGLFGVYGEEYLPNKSIKNGIMVVDSIAYD
                     LGLQDGDKLVALDGKPIERFDNFQKDMLFDQAKKLEIERNGEKITLDISREFLGGLIQ
                     KKKMQIILPAFPFIVDSVLAGKPAAAADFQKDDKILSVNQVPTPKYSDASKEISKYPN
                     QEITIQVERNGQAKDLKVTPDSTAKVGISPSLDFKKLYQTETIHYGFFGAIARGIKEP
                     FSKLGDYWNSMKLMALPKVEIKDNLGGFYSMGKIMPATWDWRAFWTITAFISVILAFM
                     NILPIPMLDGGYIVFLFYEMIVGKPLPEKVQNALQYVGLMIVFGLLIVANGMDIIRGI
                     FG"
     gene            12524..12602
                     /locus_tag="PLS16_06395"
     tRNA            12524..12602
                     /locus_tag="PLS16_06395"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12557..12559,aa:Leu,seq:caa)
     gene            complement(12684..14015)
                     /locus_tag="PLS16_06400"
     CDS             complement(12684..14015)
                     /locus_tag="PLS16_06400"
                     /inference="COORDINATES: protein motif:HMM:NF013333.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0010142 - farnesyl diphosphate biosynthetic
                     process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HNJ60746.1"
                     /translation="MKVGIEAAHFFIPDLYLEIKDLAIERGIDSLKLEKGLGLNKMAV
                     LNSNEDTATLAANALFRLIENYAINPNDIAKIYLGTESALDAAKPTCTYAVQMVEEQL
                     VTKYGVHCFRNCDVLDMTFACIGGIDALQNALDFVQVHPNRKAIVIAADYAKYELGSS
                     GEYTQGAGAVALLISSSPNLIEIESTFGIATESVFDFFKPRRYFEKSILKNAPENYPD
                     TLEIFTDEPIFDGQYSNECFKERIKEAYYHFKSGIGTTENMLDEWQHLIFHLPYAFHG
                     KRILTELFALEKDINIEPNEALKQLASSDLYQEFVKEKIETSQKASSEIGNMYTASIF
                     MALLSTFQTAFDEGKAITENKVGFFAYGSGSKAKVFEGKINKNWQSVVAKWHLFQSLA
                     NRTPIDFKTYLQLHKKQITSVIHQKPNQFVLDSIEKENPNLKGARYYTYIR"
     gene            complement(14170..15513)
                     /locus_tag="PLS16_06405"
     CDS             complement(14170..15513)
                     /locus_tag="PLS16_06405"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HNJ60747.1"
                     /translation="MRTNMFRILFALVVFTSVTAITSCKKDKFDEPPITIPEPKEKDS
                     VNTTIAELKALYTTMGTPLEITDNLWIQGVVVGDDRTGNVYKQIVIDDGTAGISISLD
                     QSDSYVNYPVGRKVYVKCKGLYLGDYNKLIQLGGGVDPDDATKTARIASALISKIIIK
                     GPAGNTVTPIEVKINDLNSSYQNRLIVIKNAEVVPGDTTKTYANFATQQAANVTFKDC
                     DGNTIIARTSGFADFAGKKVSKGNGDLVAIYTEFGSTPQLLLRSEADAKLEGPRCPEP
                     VVLLSENFDAGGTGNITIPGWVNFSTAGLKTWYATGSTNKNARFSAFNSTPANQETSN
                     IGWLITPAINFDASTNEELNFRRYVGFASGTIKMEVLYSTNYSGSGDPSAATWTLIVD
                     DAPLATSGFSDAGPISLSTISGTAVHIAFRYTGGYGLSPAATTQYNIDEIEITGE"
BASE COUNT         5485 a         2304 c         2556 g         5209 t
ORIGIN      
        1 agtacaaaac ggaaacaaca actaaaatat ctaagactat cagtggtgca agcactgtca
       61 ttaaattata attcccatga tgcaaaaaca ttttctacca attttattca ttacgtttct
      121 aattagtttc ttttccttgt cttcttgtac ctacgaaaat atgcctattc cagatattga
      181 gcaatatacg attgataccg taatgactcc aaccgataca tcaggatata tttatggaag
      241 gatttatgaa aataaatcag agcaaggacg aatttttgaa gcatatacgg caacatctta
      301 cttaaatggc gatacaattc tatcaattag cacgacctat aagaatcaaa aattatatat
      361 cctttttaat tctaatcaat taggaaaata tactgcatcg ccagatgttt ctaatccgat
      421 gtcatgtttt attttatttg atagcaatgt ttctaatctt acagatttgc tagtgaatct
      481 tagagttgga gagataaagc ttaactcttt tgatagcgta aataatattt ttagaggaaa
      541 aattaatgtg agcaatattg gttttggaac gaatacttat gacattcgag gagacattct
      601 tctaaaaatt aatttgactt cctttccaac tacgccttaa ctttttcaaa atattgccaa
      661 attacatcat cttttggaac aggagcaatg atagaaattt ctttcatact tataggatgg
      721 ataaattcta tgctccttgc atgtaaatga atagaagcat tttcattagg tcgctcaaaa
      781 ccatatttta aatctccttt tataggagaa ccaattttgc tcaattgtgc tctaatttga
      841 tgaaatcttc cagtagttgg atagacttcc aataaatgat atttatctga tgatgttaaa
      901 tatttatatt ctagttcaca aattttagaa tgggcaactt ctttattaaa aagcttagct
      961 ctttttcgtg cctcgtcttt ttgatgataa tgaattaaag tagccgtgtt aacctctggt
     1021 ttgtttttta caacagccca ataagtcttt tttacatcac gtgttctaaa taagtttgtc
     1081 attcgagaaa gagccttgct tgttcttgca aacacaacga caccacttac aggtcggtca
     1141 atacgatgca caacacccaa aaatacttcg ccaggcttat tatatttttc tttcaaatat
     1201 cgtttaaccg gttctgttaa aggttcatct tgggtaatat ctgcttgtac aatatctcct
     1261 gattttttat ttacaatgat aatatgatta tcttcgtata atacttctaa agccatagct
     1321 caaaaataat caaaaatgaa taaacagcca gttgtaattt taggaatgca tcgtagtgga
     1381 acgactatga taactaaaat attggaaaat ctaggcttat atgttggtga ggagaaagaa
     1441 gaaaaccacg aagcattatt cttttgggaa ataaacaatt ggatttttga cttgcataca
     1501 gctcgacctg agttgccaca caatatgcaa tttaccaatc caaaaacaaa acaaattata
     1561 gaagaatcct tgcaatattt tgttcaatca ccaagaaaaa agaaatatct aggaagccta
     1621 aacaccaact accaatcact tcaagcatta gattttaact ttggctggaa agaccctaaa
     1681 aatactttta ccttagagtt ttggaaatct atttttccta acccaaaaat tatccatgtt
     1741 tatagaaatc ctatagattg tatttccagt tatattgaaa gagatttggt gatgaaaaat
     1801 caattccaat gggattggaa gaaaaaagtg aaacgaaggt ttttgattac acataaattt
     1861 aattataatt ttcgtttgta taatttagaa cagggttatc aactttggca agaatatgtc
     1921 caaaaagcaa ttgacttaca aaaggataat tccaattatt tagctattca atatgaacaa
     1981 tttttgaagc aacctgaaat tgaaatccaa aagatgttag cgttttgcaa tttatctcca
     2041 tcagaacaag tcattcaaca acaaataaat accattcaaa aagatagagc ttatgctttt
     2101 gtttctaatg aaaaatatgt agatttctat caaactatta aaaatgataa attgattggt
     2161 caattagatt atcagaatat cgtgtaattg atgcaggaaa aagtagatat attattggca
     2221 acttataatg gagaaaggtt tcttcagaaa caaatcgatt ctattctaca acaaacctat
     2281 tctaatttta caatttatat tagagatgat ggctcaaaag atggtactat aaaaatcatt
     2341 aaagattatg cacagaagta tcctaataaa ataatattta tagaagatat cttaggaaac
     2401 ttaggcgtta cacagaattt taacgagttg atgaaatata gctctgctaa ttatattgca
     2461 ttttcagatc aagatgatat ttggttgcct aataaaatag aacaatcttt aaaggtatta
     2521 gccgatttag aaaacaaaca tcaaggaact cccgcttttg tttatagcga tatgcaaatt
     2581 attaatgaag aggatcagtt tacatatcct tctttttgga aattagcctc tttgtctcca
     2641 aagtacttta catttaatag acttttgatg cagagtattc ctcatgggtg tacgattttg
     2701 atgaataaaa gactaaaaga aatagccttt cctattccac aaaatgctat tttacacgac
     2761 cattggttgt ctttggttac tgtaaatttt ggaatcacaa tgccaattct cgaaccgctt
     2821 attttattaa gaaatcacgg agcaaacctt actcaaaaaa agacaaataa ttttaaaagg
     2881 cttcaacgtt tttataaaaa ttttggaact aatgatgagt atttaaaaca tctaaatctc
     2941 agaattgagc aaggaaaatc attttacaat gaatatttta atcaattaag cgttgagaat
     3001 caacatattt tagaacagtt tttattatta gagacaacat ctggctggaa acggaaaatg
     3061 atttatatca aaaataaatt ttatagaacg acattttttc atacgctgaa aatgatttgg
     3121 agagcataat ttattaaatt taaataatgg aaaatataaa aaaatgtgat tggtgtttgg
     3181 ggaaaccact ttatgaaaaa tatcatgatg aagaatgggg agttccagtt tttgacgacc
     3241 aaaagcagtt tgaattttta gtgttagaat ctgcacaagc tggattaagt tggtacacta
     3301 ttttacaaag aagagaaggt tatagaaaag cttttgtaaa ttttgactat aaaaaaattg
     3361 ccaagttttc atcagaaaag atagaagaat taatgcagtt tgaaggtatt atccgtaatc
     3421 gtaaaaaaat tgaatctgcc atcaataatg ctcaaagatt tatggaaatc caaaaagaat
     3481 ttggttcatt ttccaattat atatggcgtt ttgttgatgg aaaatctatt gtaaatcaat
     3541 tcaaaacctt aaaagatgtt ccagcaacaa caccaatttc agatgcttta gcaaaagata
     3601 tgaaaaaacg aggattcaaa tttttaggtt caacgatact ctatgctcac atgcaagcaa
     3661 cgggtttagt aaatgaccat ttgcttgatt gccaccgaca ccaacaatgt aaaatcaaat
     3721 aaatgcaaat tccatttttc gatatccgca gacaatatca tgctataaag caagaaatag
     3781 atgaagctgt tttggtatgt ttagaaaatg gacaatttat tggaggagat gaagtaaaaa
     3841 aatttgagca taatttggcg gaatatcttc aaattaatca tgtgattgct tgtggaaatg
     3901 gaactgatgc actgcaattg gctctaatga gtttgggttt gccgataggc tcaaaaatta
     3961 tcattccagc ttttacttat atcgcaccta tcgaagttgc caagttttta ggatatgaaa
     4021 ttatttttgc tgatgttgat aaacagacat tcaacattaa tttagaacat attaaaaaag
     4081 tttatactga tgatgttagg gctgttgtcg ttgttcattt atttggacaa atttgttcag
     4141 atattgatga gataaaccaa ttttgcttgg ataaaaatat tttcttgata gaagataatg
     4201 ctcaaagtat atcagctgaa aaaataataa atagaaattc tttaatgacg acttcatttt
     4261 ttcccaccaa aaatttgggg gcttttggtg atggtggtgc cgtattaacc aacgacgaaa
     4321 ttttatcaaa aaaaattagg caaattgcct cacatggtca aactaaaaaa tatgagcata
     4381 caattattgg tatcaattca cgtttagata ctttacaagc tgctattttg aatattaaac
     4441 tacaatattt agacaggaat attgaaaaga gaaaagaaat tgcaaatcta tataataaag
     4501 cttttgcatc tattcctcag attgaaacac caaaagattt tggacgacat acctttcatc
     4561 aatatacctt aaaagttgat gaggaacatc gagataattt gaaagaatat ttgaaacaaa
     4621 atggaattga aaccaatatt tattatccaa ttcctgcaca tttacaatct gcctatttac
     4681 aaagtatttc actgccaaat actgaaatgt tatgcaaaac agtactttcc ttacctattt
     4741 ttcctgaatt aaaacctaat gagcaattat atattatcaa aaatatcctt gattattttc
     4801 aaaaatagtt caatacacag cttttagatt gtttaacaca gcctataagc ttcaataact
     4861 tttatttaag taactttgca acattgaaat taaacaatac tcaacaaaag catagctgag
     4921 ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnattgccaa gcaatctcca
     5041 attatgaagt ctgtagcaaa acatgctgct gatggaggaa tcgtttgggg aatttgcaat
     5101 ggattccaga ttttatgtga agcaggattg cttcctggtg cattattgat gaacgaccat
     5161 caaaagtttg tttgccaaaa tcaatatata aaaccacaaa ctttaaatag cttaattact
     5221 agaaatctgg atttgaataa agcatataat gttccaattg ctcatggcga aggtagatat
     5281 tttgcagatg aagcaacttt gaatgaaatg acagtaaatg accaaatcct atttcactat
     5341 tgtgatgaaa acggcaatat caacgaacaa agtaatccaa acggttctac aagaaatata
     5401 gcaggaattt gtaataaaaa gagaaatgta tttggaatga tgcctcaccc agaacgtgct
     5461 tctgaaaaag tattaggaaa taccgatgga ttggctcttt ttgaatcgtt acttttgtta
     5521 caaacagcac tataaaaaca tcggatattt gcagcattaa aatttttcta aatgaaacgt
     5581 atattacaat atactaattt cctgatttta ttatttttta gcctatactt gaatgcacaa
     5641 ggaatgcaaa gtcatttgtc tttcaaaact tcagtagaga cacttggtaa taatgaatat
     5701 aatttaaaaa tcacttgtac cttagataaa gattggcatg tattttctca atttacagat
     5761 gatggcggac cattgccaac ccgatttata tttaaaaata acaaagacat acaacttatt
     5821 ggaggggtaa aagaagttgg aaagttgcat aaaaaatttg atgaactatt tggtgtaaat
     5881 gtttcatctt ttgacaatca agttacattt gtacaaaagg taaaagtaag caatcccaaa
     5941 gctattttgg aaggagaatt tgatggacaa gtttgtaaag acgaagaggg ttgtatgcct
     6001 tttggtcctg aaaaattaaa ttttagattt gataatacag aggaggctaa aacaaatgat
     6061 actattagtt ctagtttagt acaagtagat acctcaaagt ctgaagatac gacagcattg
     6121 aggagtgata aattcaattg gcaacatgcg gataatggat gtagtgtaca gtcagaaaaa
     6181 aaggacaaga gtatgttatt aattttcctt tttggattta ttggtggctt agttgcttta
     6241 ttaacacctt gtgttttccc gatggtacct ttaactgtaa gcttctttac taaaggtgga
     6301 aaagataaaa aacaaggtat taaaaaggca ctcacttatg gcttatcgat aattgtgatt
     6361 tatgtacttt taggattagt cattacatca ttgtttggct cagatgcttt aaatgcaatg
     6421 agtacaaatg tttggtttaa tatggcattt tttgtcattt ttgtcgtatt tgcattttct
     6481 ttctttggat tttatgaaat tactttgcca tcatcttggg caaatcaatc tgatagttta
     6541 gctagtaaag gtggcaactt aggaattttc ttcatggcat ttacattagc tttagtttct
     6601 ttttcttgca ctggtccaat cattggtact ttattagtgg aagctgcaac aggtggaggc
     6661 ccaacaattc ttggaagaat tcccataaag ccaacaattg gaatgttagg tttctcaaca
     6721 gcattagcat tgccatttac tttatttgct ttgtttccac aatggttaca atctttgcca
     6781 aaatctggtg gttggatgag taccgtaaaa gttaccttag gatttattga attagcatta
     6841 gcactcaaat tcttatctat tgcagatatg acacaaaatt ggggtatttt gagaatagag
     6901 ccatttttga ttatctggat aatattattt attgtcttgg caatgtattt attgggtgta
     6961 ttccaatatt ttaaaaaaga aacaaaagaa catatttcta taccaagaaa gctatttgga
     7021 cttgcttcat tgggttttgc tatttatatg gcttatggtt tatttactta tcaaccatta
     7081 aaactattaa gtggattggc tccgcctgtt ggctataatt tcaaaaacga gaaaaacgaa
     7141 actttcatac attttaaaga ttatgatgaa ggattagctt atgccaaaac acataatctg
     7201 cccatattat tagattttac aggatttggt tgtgtgaatt gccgaaaagt agaggaacac
     7261 gtttggacaa atcctacaat tgataaactt ttacataaat atgtcatcat ttcgctttat
     7321 gttgatgata gaaaagaatt accacaagac caatggtacg aatcagatgc tactggaaaa
     7381 ttacgagaag ttaaaacgat aggacaaaaa tggagcgatt ttcaagcttt acatttcaaa
     7441 acaaatgcac aaccttacta tgttttgata gataaagaag agcgaatttt aaatcaacca
     7501 gtagattatt ctttttcttc ctcagtagcg aattacaaaa atttcttaga atgtggtttg
     7561 aatatggaaa agcaactaaa tcattaaatc agattttttt aggataaata gcttgttata
     7621 aatcataaaa atcagataaa aaactagtaa attaaagatt ttgtttttaa tttattaact
     7681 ttgtgtactt aaagtggaaa agtgccaaca cgtttttgta agaaatatat atttttagta
     7741 atttttgttt ttgtcggaat gatgaaaggc tatgctcaag acccatcttt tgcacagttt
     7801 ttaaatttta gacaatacct caatccagca ttaactggct cagaaagagg cttgaatgta
     7861 tctatgattt ataaaaatca gtggttttat gttccaggag gctttaatac gtatggaatt
     7921 gcagctgatg tacaaagttc aagactgtca tcaggaattg gaattattgc ttatcgtgat
     7981 gtagaagcaa aagcacttac tagaaattat gttggagcat cttatgctta tatcgttcgc
     8041 ttatcaaaaa atgctaattt acattttgga attagagcag cgtttgtgaa taacggatta
     8101 gaccgtacaa aattattatt ttccgaccaa atagatcctg aaattggtgc aagtggagga
     8161 acgccctcaa ttacaacaat tcccaatcgt acagtaaatt attttgacct agatgctgga
     8221 acagtaatgc gatttagatt tgatatcaat gaccgtcctg ttcataattc tattggattt
     8281 gcggtaagtc atcttacacg accagatgaa tcttttttga atatagaatc aaaaattcct
     8341 atgcgattta ctttgcatta cggaagcatg ttcccaattt attcaaaaaa aatgtacaat
     8401 cgagacccaa tgttttatat ttctccagtc tttaaatttg attatcaatc aaaattgagg
     8461 gtgtataatg ctgggtttta tactacattc aaaccaattt atactggaat aatgtatcaa
     8521 ttaaataaat accgatttga caatacacat ggactgatta ttttaggtgg tattgattgg
     8581 gatttaggtt atgacgaaat gatgacttta aatatcggtt acagttatca aattgatttt
     8641 tcaggtatag gtacaaaaag ccgtggacag cacgaaatta cactcagaat gaatttcaat
     8701 gatgtagcta tcaaaaatcc aaagaagaaa gcaaaagcca tagattgtta ttctttccct
     8761 ggcagaaaag cggttaaaat tttctaataa ttttcttttt tttatagatt aggtgttcgg
     8821 taaattgttg aaaacgacaa tattttttaa ttttacaaga gtgattactc aagataccat
     8881 acggaaaatt atagatacag cacaagcatt agatgtggtg gctgattttg taaccctaaa
     8941 aaaatcagga agtatttata aaggaaattg tccatttcat caagagcgaa cgccttcatt
     9001 tgtggtaaat ccaaataaaa atatctttaa atgttttggt tgtgggaaag gtggagatgc
     9061 gattggtttt attatggaat atgaaaaact atcctatcca gaagcattaa gatatttggc
     9121 acaaaagtat aatattgagg ttgaagaaac acatactcaa agtgaagaaa gcaaggagga
     9181 gcaatctcaa atagagagtt tgtatatcgc tttaaatttt gcagcaaagt tttatcaaca
     9241 acaattgaca gaatctgaag atggtagaaa tattgctttg agttattgta aagaaagagg
     9301 cttttcaaac gaaacaataa ctacctttca aattggttat tcgcctgata gctttgatgc
     9361 cttttataaa gaagcaaaat caaatgcctt taatgaagat atcttactca aagctggatt
     9421 aatcaaagaa aagaacgaaa aacgatacga cttctttaga gatagattga tgtttccaat
     9481 ttggaatgtt tcaggaaaag taattgcttt tggtggtaga attttaaaaa agattgacaa
     9541 tcaaccaaaa tatatcaata ctgccgaaac agatgtgtac cacaaatcac agattttgta
     9601 tggtattttt caagcaaagt ccgatattcg gaaaaatgat gcttgttttt tggtagaagg
     9661 atataccgat gtcatttctt tacatcaatc gggaataaaa aatgtggtgg caagttctgg
     9721 aactgcattg acaaaagagc aagttttttt gataaaaaga tttacagaaa atattattat
     9781 tctttatgat ggcgatgccg caggcattaa agcagctttg cgtggattag atattgtttt
     9841 agaactcggc ttaaatgtaa aaattgtttt attaccagat ggagaagatc cagattcttt
     9901 tgtaaagcaa aaaggaaaag aagaggcact tcaatttatt aaagaaaatc agcaagattt
     9961 tattcttttc aaagcatctc aaagcatctc ggatgtaaaa aacgacccta tacaaaaagc
    10021 agaagcgatt aaagatattg tccgtagtat tactctaatt caagataata tcaaacgaca
    10081 attatatata aaagaatgtg caacgattgt tgatattgat gaaaaaacat tgattaatga
    10141 agttaataaa attaagattc agcaaattaa gaaaagagaa aatgttcctg aagaagatgt
    10201 agagcagttg gcttcacttc aagagaaata tatcgaacca caaaaaaaag aatcacaaat
    10261 tccagaattt ttctatcaag aacgtgatat tattcgagtt ttattagaac atgcggataa
    10321 aagcatgaat gaagaagata atgttggagc ttatatatta tcggaattag tagaagtgcc
    10381 ttttaaaaat acgatttttg ataaaattat caaacattat atcaatgcat acgaacaaga
    10441 tattccgttt gataccataa gtgatgcaac caaaattgaa gataatcaaa tcaaattagc
    10501 tattattgat attcaatctt ctaaatatga aattagtgag aactggctaa aaaagcatga
    10561 aattattatc actgaagcca atagaaccta caaaactgat gttgcatctg tgttgaatcg
    10621 ttataaatat ttgaaattta tggaattgct taaagattta gatgaaaaaa tcaaattagc
    10681 agatacagaa aataaaatgg aagaactata ttcttcttta caaaaaaaga taaaacttat
    10741 aggtgtccga aatgagttgg caaaagaaat aggtacagta actcatcctt attaaattta
    10801 agcatcatct tcacaaataa cattgataat tttatccatt atttttaaaa ttctatattt
    10861 tgagaaaaat agcctaattc ttaaagttaa aattcaaaat ttaaggctaa tatttcgtgc
    10921 aaatatcctt gcttacctca tctgaacata aaataaataa taattaacgt attgaaagtt
    10981 atggttttcg tacctttgtc cactagatta aaaattaaaa aatacagatt ataataaaat
    11041 gaatttaata caagtattct atcttttcct tggactgtca ttgttgatta ttctacacga
    11101 atttgggcat tatcttccag caaaattatt taaaaccaga gttagaaaat tttacttatt
    11161 ttttgatttc ttattccctt tcagcaaagt attgccattt tctattttta agaaaaaaat
    11221 aggagatacg gaatatggct taggttggtt tccattcggt ggatatgtag atattgctgg
    11281 aatggtagat gaaacaacct ctgcagaaga tttggaacaa gatgatacgc caaaagaaaa
    11341 tcaattccgt tttaaaccag cttggcaacg actgattatt atggttggtg gtgtaacagt
    11401 gaatatggtt ttggcgatgg tcatttatgc tggattgttt ggcgtttatg gcgaagaata
    11461 tttaccaaat aaatccataa aaaatggaat tatggtcgta gattctattg cttacgattt
    11521 agggttgcaa gatggtgata aattagttgc tttagatggc aaaccaatcg agcgttttga
    11581 taattttcaa aaagatatgt tgtttgacca agctaagaaa ttagaaattg aacgaaatgg
    11641 cgagaagatt acattggata tttcaagaga atttttgggc ggcttaatcc aaaagaagaa
    11701 aatgcaaatt attttgcctg catttccttt tattgtagat agtgtattag ctggtaagcc
    11761 agcagcggcg gcagattttc aaaaagatga taaaatattg agtgtaaatc aagttcctac
    11821 tccaaaatat agcgatgcat ctaaagaaat tagtaagtat ccaaatcaag aaattactat
    11881 acaggtggaa agaaatggac aagcaaaaga cctaaaagta acaccagata gtactgcaaa
    11941 agttggcatt tccccaagtt tagatttcaa aaaattatat caaacagaaa cgatacatta
    12001 tggatttttt ggggcaattg caagaggcat caaagaacca ttttcaaaat taggcgatta
    12061 ttggaatagt atgaagttaa tggcattgcc taaagttgaa attaaagata atttaggtgg
    12121 tttttattct atgggtaaaa ttatgccagc aacttgggat tggagagcat tttggacaat
    12181 aactgcattc atttctgtaa tcttagcgtt tatgaatatt ttaccaattc ctatgttaga
    12241 tggtggatat attgtgtttt tattctatga aatgattgta ggcaaaccat tgcctgaaaa
    12301 agtacagaat gctttgcaat atgtgggtct aatgatagtc tttggattat taattgtcgc
    12361 caacggaatg gatattattc gtggtatttt tggctaaaag tattttgata tatcaaaata
    12421 taaaattact tttgcggtca gttctttgat taaatagtat tagcaaaata gcaacggata
    12481 aaaacgttta tttttgttgc actatttctt cattaatcag tcagccgaag tggcggaatg
    12541 gtagacgcgc acgactcaaa ctcgtgtacg aaagtgtgtg ggttcgagtc ccaccttcgg
    12601 tacaaacaac aaaactaaag ccttgtaagt aaacgaatta caaggctttt ttttgggtct
    12661 attgtagaaa ggataactaa cgtttatcga atgtaggtat aatatctggc accctttaaa
    12721 tttggatttt ctttttcaat gctatccaat acaaattgat ttggtttttg atgaatgact
    12781 gaagtaattt gctttttgtg taattgcaga taggtcttaa aatcaattgg agtacggttt
    12841 gctaaacttt ggaataaatg ccattttgca acgacagatt gccaattttt attgattttt
    12901 ccttcaaaaa ccttagcttt tgaaccgctg ccataagcaa agaaaccaac tttattttca
    12961 gtaattgctt tgccttcatc aaaagcagtt tgaaaagtgg agagcaatgc cataaaaatc
    13021 gaagcagtgt acatattccc aatttcagac gaagcctttt gtgaagtctc aattttttct
    13081 ttgacaaatt cttggtataa atcagatgat gccaattgtt ttaaagcttc attgggttca
    13141 atattaatat ctttttccaa agcaaagagt tctgtaagta ttcttttacc gtgaaatgca
    13201 taaggcaaat gaaaaatcaa atgttgccat tcatcaagca tattttcagt agttccaatt
    13261 ccagatttga aatgataata ggcttcttta attcgctctt taaagcattc gttagaatat
    13321 tgtccatcaa agatgggttc atctgtaaat atttcaagtg tatcaggata attttctggt
    13381 gcatttttta aaatactctt ctcaaaatat cttctaggct taaaaaaatc aaaaacactt
    13441 tctgttgcaa ttccaaaagt gctttcaatt tctattaaat ttggggaaga tgaaatcaat
    13501 aaggcaacgg ctccagcacc ttgtgtatat tctccactgc tacctaattc gtatttggca
    13561 taatctgcag caataacaat tgcctttcta tttggatgta cttgtacaaa atctaaagca
    13621 ttttgcaagg catcaattcc gccaatacaa gcaaatgtca tatctaaaac atcacaattt
    13681 ctaaagcaat gaacaccata ttttgtaact aattgttctt ctaccatctg aactgcataa
    13741 gtacatgtag gttttgcagc atctaaagca ctttcagttc ctagataaat ttttgcaata
    13801 tcattaggat tgatggcata attttctatc aaacggaata gagcattggc tgctaatgta
    13861 gcagtatctt cattgctatt taaaactgcc attttattta agcctaaacc tttttctaat
    13921 ttcaaagaat ctatgcctct ttcaattgcc aaatccttaa tttctaaata caaatctgga
    13981 ataaagaaat gtgccgcttc aatacctact ttcatgtgct ttggttattt atgtgcttac
    14041 gaaaatagaa cctttaggtt taaatcaaag tcaataaatc ttgaaactta taatattaaa
    14101 tttcactaca aaaaaaagag gctgtcaatt ttttaggaca gcctcttttt ttataaaatt
    14161 agaatctaat tattctccag taatttcaat ttcatcaata ttatattgtg tcgttgctgc
    14221 tggtgataaa ccataaccgc cagtatatct aaaggcaata tgaacagcag ttccagaaat
    14281 cgtacttaaa gaaattggac cagcatcaga aaatcctgaa gtagcaagtg gtgcatcatc
    14341 tacaattaaa gtccaagttg cagcacttgg gtctccactg cctgaataat ttgttgaata
    14401 taatacttcc attttgatgg taccagaagc aaaacctaca tatcttctaa agtttaattc
    14461 ttcatttgta gatgcatcaa aattgatagc aggagttatt aaccaaccaa tattagatgt
    14521 ttcttgattt gctggggtgc tattaaatgc tgagaaacgt gcatttttat ttgtactacc
    14581 cgtagcatac caagttttca aacctgctgt ggagaaattt acccaacctg gaattgtaat
    14641 atttccagtt cctccagcat caaaattttc agataagagt acaacaggct cagggcatct
    14701 aggtccttct agttttgcat ctgcttcact tcttaataat aattgtggtg tagaaccaaa
    14761 ttcagtataa atagctacta aatcgccatt tcctttgctt acttttttgc ctgcaaaatc
    14821 tgcaaaacca ctagttcttg caataatggt attgccatca caatctttga aagtaacatt
    14881 tgctgcttgt tgtgttgcaa aatttgcata agtttttgtt gtgtctccag gtacaacttc
    14941 agcattttta attacgatta atctattttg ataagagcta tttaagtcat tgatttttac
    15001 ctctattgga gttaccgtat ttccagctgg tcctttaatg atgattttgc taatcaaagc
    15061 acttgcaata cgtgcagttt tggtagcatc atcaggatca acaccaccgc ctaattggat
    15121 aagtttattg taatcaccaa ggtataaacc tttacatttt acatatactt ttctaccaac
    15181 aggatagttt acataagaat cactttggtc taaagagatt gaaatacccg cagttccatc
    15241 atcaatgacg atttgtttgt acacattacc agttctatca tcaccaacta cgacaccttg
    15301 aatccataag ttgtctgtaa tttctaatgg agtacccatt gttgtatata atgcttttaa
    15361 ttcagcaata gtggtgttta cactatcctt ttcttttggt tcaggaatag tgatgggtgg
    15421 ttcatcaaac ttatcttttt tacaagatgt aatggcagtt accgatgtaa agaccaccaa
    15481 cgcaaataaa attctaaaca tatttgttct cattattgat attttttctt tttattatta
    15541 atacattctc caagctaaac tgataaaata attgatgcca taagcatacg catatctatt
    15601 tttaaataga tttaagtctt tgccgtccac atcaaatctt aattgttcga aagc
//
LOCUS       DAOQCW010000056        15458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1384_length_15458_cov_4.777121, whole genome shotgun sequence.
ACCESSION   DAOQCW010000056 DAOQCW010000000
VERSION     DAOQCW010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15458
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1384_length_15458_cov_4.777121"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PLS16_06410"
     CDS             complement(<1..268)
                     /locus_tag="PLS16_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNJ60748.1"
                     /translation="MENKVAIVSGANGNLGRAVVQYFLAQKWLVIGLEHRLCNSIIEE
                     NYQEIEVNLLDENATYNCVNKIITDYGRIDALVHTVGGFAMGNIA"
     gene            complement(325..795)
                     /locus_tag="PLS16_06415"
     CDS             complement(325..795)
                     /locus_tag="PLS16_06415"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNJ60749.1"
                     /translation="MLELLSKKDVQTIRILQDFNVSTERVFSFFSDHNRLSEIYPAFI
                     HRIVDAPNPFNCNDVGSSRIIIGFPVILQETITAYIENKYIEYKISSVSPLKNHIGKM
                     NFIPLSENQSRLEYVIEFEPLVPFTGFLLLQLNTKIVQDALGVLVRRFKENPNY"
     gene            complement(795..1217)
                     /locus_tag="PLS16_06420"
     CDS             complement(795..1217)
                     /locus_tag="PLS16_06420"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNJ60750.1"
                     /translation="MQSIDITQKFNAPQQEIFNFLSNHNNLKSIFMIDVKRIKDAEGD
                     NKNGLGSVRSLGIGVGLIEETVTVFEAPNLIEYKISNNIPVNYHLGRLEFSSENNQTV
                     LHYTIDLESKFAFADPILKFILETSIRNGLSKLASKYK"
     gene            1341..1829
                     /gene="rraA"
                     /locus_tag="PLS16_06425"
     CDS             1341..1829
                     /gene="rraA"
                     /locus_tag="PLS16_06425"
                     /inference="COORDINATES: protein motif:HMM:NF006875.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HNJ60751.1"
                     /translation="MSFATADLYDANENNPNIQVALPLFQSYGKKKQFNGKIFTLKCH
                     EDNTLVGDILRKEDGTGKVLVIDGGGSLRCALVGDNIALAAIKNNWEGIIVYGCIRDA
                     APINDMKIGIRALATNPTKTVKRNTGIQNITVNFANVNFINEQYVYVDEDGILVSDKA
                     LI"
     gene            complement(1830..3851)
                     /locus_tag="PLS16_06430"
     CDS             complement(1830..3851)
                     /locus_tag="PLS16_06430"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HNJ60752.1"
                     /translation="MIIKFKQFSLIYYKEDKQISIQNQDFSQFEHEDFVLEFLLEADE
                     DKNATIYTVELMPKQAIRLVHFSIDIDTDYANTFGVFANGFQSWTESRVYYKDDIIKP
                     AKKFIKGLAYTYGDYNFVNYKKHKGCIHSWNYTYIKQEKYKINLLASLNETNAYTLFE
                     HDMQNNSLRISKEIEDRQVAEKFELLHFVSIEDREDFAFQNFAFIKSFKTLKVQPSIG
                     WTSWYNYYTKIDEQIILDNLENFTQNNIPISIFQIDDGWQNAVGDWLNIQPKFKNGMQ
                     FIADKIKSKNITAGLWLAPFACEKNSFIFREKQDWVLKDKTGKLIKIGYNPMWSGWFY
                     ALDFFNEELRDYLRKVFDTILNEWHFDLVKLDFLYGTASIPQHGKSRGEVMHLVMNFL
                     RECVGDKKILGCGVPLSAAQDTTDFCRIGPDIHLSWEFKLLKLLNARERLSTYSAIHN
                     TISRRHLNQLGFINDPDVFILRKEKNNLNFNEQYSLLLANLLFGDLLFTSDDISKYNE
                     ETLFLYKSIFPLVEKESVLVQQEKEFYQIHFNIGDKYYLALINTANHDKIYKLPNGSF
                     FDNVTQNTLKGNKGLTIPKHSSKCLFRINATPFTLVGSKGHFFCGVEVDNIFISGGIL
                     EIEWNKKLVNKPIVYYRIPISYEISLSENTQLESRTEDADFAIIALSPK"
     gene            3970..4860
                     /locus_tag="PLS16_06435"
     CDS             3970..4860
                     /locus_tag="PLS16_06435"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HNJ60753.1"
                     /translation="MKIEQITVIGAGTMGNGIAHVCAQFGYKVNLVDVSETALDKAIT
                     TITKNLDRQLQKGSITEETKNLTLNNITINTDLKTAKNSDIVIEAASENFNIKEKIFQ
                     QLDTICKPETILASNTSSISITRIAACTNRPEKVIGMHFMNPVPVMKLVEIIKGYSTD
                     KATTETIVTLTKQLQKTPIEANDFPGFVANRILMPMINEAIYTLNEGVSGVAEIDQIM
                     KLGMAHPMGPLQLADFIGLDVCLSILNVLYEGFGLPKYAPCPLLVNMVTAKHLGVKTG
                     KGFYDYSGGTKELVVSSNFS"
     gene            4922..5752
                     /locus_tag="PLS16_06440"
     CDS             4922..5752
                     /locus_tag="PLS16_06440"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNJ60754.1"
                     /translation="MSNILVPLDFSPNAINALKYADNLAKSIDANLIVLNIYDGGISN
                     YEAGWPIEELFDNVVKMIQEKTNAIIKENCSSTNISTVFKNGNVKDLILESIDLLGAN
                     FVVMGTHGASLITKVLFGSNTAHTIAHSKVPVLAVPSDFSFTPLQKIVYASDFNNLNA
                     ELKLLNKVSEQLKNIPIETLHLRYETESAENISENFKKVISENNYQNIQLQQETFDLD
                     NTLANAIREYAEAQQNIILAMFAEDRNIFQSLFLGSKTEYLTYKLNVPLLAMNKANLS
                     "
     gene            complement(5901..7154)
                     /locus_tag="PLS16_06445"
     CDS             complement(5901..7154)
                     /locus_tag="PLS16_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNJ60755.1"
                     /translation="MNLTLAERMRPKQIEDLIGQQHLLGKGKPFRKLIENGALHSLIL
                     WGPPGVGKTTLARLLAENAKLPFEQLSAIAAGVKELRAIIDEARTFGTKVLFIDEIHR
                     FNKSQQDALLGAVENGTIILIGATTENPSFEVNAALLSRCQVYILNSLEADELKHLVD
                     KALKEDANLVAKNIQVAEYEALIYFSGGDARKLLNVLEIVADNLEINTIQNATVEAII
                     QAQIARYDKKGEQHYDIISAFIKSMRGSDVNAAVYWLARMIVAGEDPKFIARRMLIFA
                     SEDIGNANPNALLLANATFDAVSKIGSPESRIILSQCVVYLATSVKSNASYLAINDAI
                     ALAEKTNHLSVPLHLRNAPTKLMENLNYGKGYKYAHQYENNFIDLEFLPEQISNTKFY
                     EPGINAKENELRRFLKERWKEKYGY"
     gene            complement(7156..7668)
                     /locus_tag="PLS16_06450"
     CDS             complement(7156..7668)
                     /locus_tag="PLS16_06450"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNJ60756.1"
                     /translation="METIRKCKDTANVQTELIMPGHTNPIGNLMGGNLLCWMDVCSGI
                     SAIKMSRHIAVTASLDDVSFKLPIKVGDIVILRSFVTRSFNTSMEVICEVFIKNIRNN
                     EELKSHEAYFTFVALNDDGTPVKVEQVEPETEREKELYENALERRELRLVRAGKMKMV
                     DTVVLKNKLV"
     gene            7743..7819
                     /locus_tag="PLS16_06455"
     tRNA            7743..7819
                     /locus_tag="PLS16_06455"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7777..7779,aa:Arg,seq:tcg)
     gene            7833..8714
                     /locus_tag="PLS16_06460"
     CDS             7833..8714
                     /locus_tag="PLS16_06460"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNJ60757.1"
                     /translation="MLLIPSPLERILLPFKNNITLYIKRDDLIHSHISGNKWRKLKYN
                     IEAFQQQNKKILVTVGGAFSNHIMATAAVSNWKKIPCIGIIRGDELNIHSNKNLAYAH
                     QQNMHLEFIDRHFYAKRYDDDFLELLSNKLDIPNQDIFFIPEGGANMEGAKGCEDIVH
                     EIDLNFDYILTDCGTGTTLAGIANAVHENQKAIGISVIGNNFDIEQSTRHFTNKPFTM
                     LHQFNFGGYAKTNTELLDFIKYFYRMFQIKLDYVYTAKLFYAIFNLIEQDYFPPNSTI
                     IGVHTGGVLNADIFDEI"
     gene            8704..9294
                     /locus_tag="PLS16_06465"
     CDS             8704..9294
                     /locus_tag="PLS16_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HNJ60758.1"
                     /translation="MKFNTIHSIESVIIKDKQSKFIGYAYPIYSELEAKEKIEAIKAQ
                     HPKATHHCYAYKLGFDNNNYRANDDGEPNGTAGKPILNQINSLGLTNCLVIVVRYFGG
                     TKLGVSGLIDAYKLCAKETLAATTIDEREIISTFNFEVAFELTNQVFDYLNKKQYTYT
                     KTFTKSNIQFNVQITKELEDTLINFLAQKQIEFSVE"
     gene            complement(9298..9690)
                     /locus_tag="PLS16_06470"
     CDS             complement(9298..9690)
                     /locus_tag="PLS16_06470"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HNJ60759.1"
                     /translation="MSDYLFSYGTLRMEQSHPMAGYLAKNSVLVGLGELKNAELFKID
                     WYPALIKTNNSEDIVYGDVFNVNSDDVLLKIDEYEGIGIGNPPYEYRREKVKIRTELN
                     EIEAWVYFYNFDLPNGAEKVLSGDFLNP"
     gene            9809..11554
                     /locus_tag="PLS16_06475"
     CDS             9809..11554
                     /locus_tag="PLS16_06475"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNJ60760.1"
                     /translation="MQTTIIRNGIYFDGTGAKGVKKDIIIKDNKIDAVMENAPNIEGA
                     KEVDADGHWVCPGFIDIHTHYDAELEVLPSLDESVRHGVTTVIIGNCSLSAALGKDKE
                     IVDLFARVENIPAKVLSKWIIGNIKWSNVREYYEHLESLPIGPNVASFIGHSNIRIDA
                     MGLDGAFMRPKATDAELFKMEGIVAEAMQAGYLGMSIDMLPFHRWAGEYEKKYLGISV
                     PSQRAAKSEYRRLAHVLRNHNRVLQITPNAVDKTSFATILRLSHGGFFKPSLKTSIVA
                     ALDLKSNENIYKVLKFYGALSNNVMRANMKFQTLAEPFLNYGDGPITPLFEEFPSMIK
                     AISSSEAERKKMFLDANFRKVFTKEWNHKATSVFPRALKEMWVVNAPDQSLIGKNFEQ
                     IAIEAGKDPVNHFMDLLAEYDTKLRWKCDTSNHREKIRLDLLNSEHCMPGFNDSGAHN
                     VNMAFHDGSLQMLKSVQKHPEVMSIERAIYRLTKEAADFLGIDAGDISVGKRADVVII
                     DPSKLDSDINSSPIEDFHPALDGAYRLVKRSGKVVKHVFINGIEAYSNNGVVGFHEGL
                     GKTKQFGQLLKSTWR"
     gene            11804..13000
                     /locus_tag="PLS16_06480"
     CDS             11804..13000
                     /locus_tag="PLS16_06480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HNJ60761.1"
                     /translation="MTQSVINRKFLIILLGILAALGPFSIDMYLPGFQQIAEEFNVHE
                     KAVAFTLTSYFSGIAVGQLLYGPIVDKYGRKIPLLVGLSIYMLASLGCALASSIEFLI
                     AMRLLQALGGCVGMVASNAIISDVYEKNERAKAFSYIILIMGLAPLIAPSLGSFFVAH
                     FTWHYIFYFLFIFSLSVILLISFFLPETSIYKHSNKLKFIKISKDYLEVLKNPIFLKY
                     TLASSIVMSILFAYISSAPFLFMTLYKLDNTTFSILFAVNAAGFIAGSYCNGVLTKYI
                     PYIKIAKVASVILASIALAATITYYINPQIPYKILTSGIFFILVLIGFINPNATAGSL
                     APFTNNIGIASALGGSFRMGIGAIVAAFIGSFQGSTAIILFVIIFILSIATVSLLWIK
                     TKKINH"
     gene            13131..14501
                     /gene="hemN"
                     /locus_tag="PLS16_06485"
     CDS             13131..14501
                     /gene="hemN"
                     /locus_tag="PLS16_06485"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HNJ60762.1"
                     /translation="MSNLIDKYNIPGPRYTSYPTVPYWDNNTFSFNNWEISVLKSYEE
                     SNDSEGISIYMHLPFCEQLCTFCACHKRITKRHEVETPYLESVIKEWELYLQLFNKNK
                     KTTPKLKELHLGGGTPTFFSPENLKILLDSLFSTVEIAESSEFSFEGHPNNTTKKHLE
                     VLYNLGFRRCSFGVQDYDLKVQKAINRIQPFENVQKVTDWARAIGYKSISHDLVFGLP
                     HQTWESVEATIRKTLELKPDRLAFYSYAHVPWVKGVGQRGYDENDLPSGETKRKLYEN
                     GKQLLQDLGYIEIGMDHFALEHDDLYQSLIQNKLHRNFMGYSSGKTQLMIGLGMSAIS
                     DSWYGFAQNNKTVEDYQAIVESGQIPVNHGHILTEEDLIIRKHILNLMCQLETNFNNK
                     NSFPELPEVLDKLKEMQKDGLVEISSKTVKITEKGRAFSRNVAMAFDLRMIRKQPEAR
                     IFSMTI"
     gene            14508..14828
                     /locus_tag="PLS16_06490"
     CDS             14508..14828
                     /locus_tag="PLS16_06490"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ60763.1"
                     /translation="MKEEFIYTPININHAENRFEMMVDGYMAYIEFEIHNGIYSLTHT
                     ISPKEIGGRGVASSLIEKVLIYLNAEKRQFIPICPMVINFIQKHPEWKKWIPKQYHEE
                     IDKA"
     gene            15005..>15458
                     /locus_tag="PLS16_06495"
     CDS             15005..>15458
                     /locus_tag="PLS16_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60764.1"
                     /translation="MSETTQDYHIIEKFREMILKRYEYADLKNRFELSDSITEEVVAE
                     VKNYFLTTIYPPAQERKELEDAFRDLAAYVKQPRKIWNLFGDMARAIFKFGRHFMSAL
                     KAGMDSLDSFMGAKDFEAAMTEIANKNGMKPPMSDEDYEDAMYQLDRVK"
BASE COUNT         5523 a         2404 c         2304 g         5227 t
ORIGIN      
        1 ccgcaatatt tcccattgca aatcctccaa cagtatgaac taatgcatct attctaccat
       61 aatctgtaat aattttattt acacaattat aagtagcatt ttcatctaaa agattaactt
      121 caatttcttg ataattttct tctataattg agttgcataa tcgatgttcc aatccaatta
      181 cgagccattt ttgagctaaa aaatattgca caacagctct ccctaaattt ccatttgctc
      241 cactaactat cgcaacttta ttttccatat atcgattaaa ttacttcgtt aaaaataaac
      301 aaaaatacag tgctaacaaa aaaattaata atttggattt tccttaaatc ttcgcaccaa
      361 aacgcccaaa gcatcttgaa caattttagt atttaattgc aataacaaaa atccagtaaa
      421 cggtaccaaa ggttcaaact caattacata ttctaatcga gattgatttt cactcaacgg
      481 aataaaattc attttgccaa tgtggttttt aagtggactc acagaactaa ttttatattc
      541 tatgtattta ttttcaatat atgcagtaat cgtttcttgt aaaataacag gaaaaccaat
      601 gataattctt gacgaaccta catcattgca attaaaagga tttggggcat ctacaattcg
      661 atgaataaaa gctggatata tttctgaaag tcgattatgg tcgctaaaaa aagaaaagac
      721 acgttctgta cttacattaa aatcttgtaa aatacgtatt gtttgaacat cttttttact
      781 taataattca agcattattt atattttgaa gctaattttg acaatccatt tcttatagat
      841 gtttctaata taaatttcaa aattggatca gcaaaagcaa acttactttc taaatctatg
      901 gtataatgta aaactgtttg attattttca gaagaaaatt ccaatcttcc taaatgataa
      961 tttacaggaa tattattaga aattttatat tctatcaaat tcggtgcttc aaaaacagta
     1021 acagtttctt caattaaacc gacaccaatt cctaaacttc tgacagaacc taatccattt
     1081 ttattatcac cttcagcatc tttaattctt ttcacatcaa tcataaatat tgacttcaaa
     1141 ttattgtgat tactcaagaa attaaatatt tcttgttgag gtgcattaaa cttttgtgta
     1201 atatcaatac tttgcataaa ccaattttct gcataaattt aagaaaaata caatcaaaaa
     1261 aacacgactt caaaacttat tttactttag ataagctttt aaaattcaat tttctatttt
     1321 atttttacag aaaaaagaca atgagttttg ctactgctga tttatacgat gctaatgaaa
     1381 acaatccaaa cattcaagtt gcattgccat tatttcaatc ttatggaaag aaaaaacaat
     1441 ttaatggtaa aatttttact ttaaaatgcc atgaagacaa cactttagta ggtgatattt
     1501 taagaaaaga agatggaacc ggaaaggtat tggttattga cggtggtggc tctttgcgtt
     1561 gtgctttggt gggcgacaat attgctttgg ctgctatcaa aaacaattgg gaaggtatta
     1621 ttgtttatgg ttgtattcgt gatgctgcac ctattaatga tatgaaaatt ggcattagag
     1681 ccttagcaac aaatccaaca aaaactgtaa agagaaatac tggaatacaa aatattacag
     1741 tcaattttgc gaatgtcaat ttcatcaatg aacaatatgt atatgttgat gaagatggta
     1801 ttttagtgag cgacaaagct ctaatttaat tacttcgggc ttaatgcaat tattgcaaaa
     1861 tcggcatctt cagttcgact ctctaattgt gtattttcag ataatgaaat ttcataactt
     1921 ataggaattc tataataaac gataggttta ttgactaatt ttttattcca ttcaatttct
     1981 aaaataccac cgctgataaa aatattatcc acctcaacac cgcagaaaaa atgccctttt
     2041 gaaccaacta aagtaaatgg cgtggcattg attctaaaca aacattttga gctgtgtttt
     2101 ggtatcgtta aacctttatt gcctttcagt gtattttgag taacattatc aaaaaaacta
     2161 ccattgggta atttataaat cttatcatgg ttcgctgtat ttatcaatgc taaataatat
     2221 ttatcaccta tattaaaatg gatttgataa aactcttttt cttgttgcac caatacactt
     2281 tctttctcta ccaaaggaaa aattgactta tataaaaaaa gtgtttcttc attgtatttt
     2341 gaaatatcat cagaagtaaa aagcaaatct ccaaacaata aattcgctaa caacaaacta
     2401 tattgttcat taaaattgag attattcttc tctttcctca gtataaaaac atctggatca
     2461 ttaataaaac caagttgatt caaatgcctt ctcgaaattg tattgtgaat ggcactataa
     2521 gtactcaatc tttctcgagc attcaataat ttcaatagtt taaactccca agacaaatgt
     2581 atatcaggtc caattctaca aaaatctgtt gtatcttgag ccgcagacaa cggaacacca
     2641 caacctaata tttttttatc acccacacac tcacgcaaaa aattcataac tagatgcatt
     2701 acttctcccc tactttttcc atgttgcgga atgcttgcag ttccatacaa aaaatctaat
     2761 tttactaaat caaaatgcca ttcatttaaa atggtatcaa aaacttttct caaataatct
     2821 ctcaactctt cattaaaaaa atctaaagca taaaaccaac cactccacat tggattataa
     2881 ccaatcttta ttaattttcc agttttatct tttaaaaccc aatcttgttt ttctctaaaa
     2941 ataaaggagt ttttttcaca agcaaatggt gctaaccaca atccagcagt aatattttta
     3001 gatttaattt tatccgcaat aaactgcatt ccatttttaa attttggttg aatattcaac
     3061 caatcaccca ctgcattttg ccaaccatcg tcaatctgaa aaatagaaat tggaatatta
     3121 ttttgagtaa aattttctag gttatctaag atgatttgct catctatttt tgtgtaataa
     3181 ttataccaac ttgtccaacc tatacttggc tgaactttca aagttttaaa cgattttata
     3241 aatgcaaaat tctgaaatgc aaaatcttct ctatcttcaa tactcacaaa atggagcagt
     3301 tcaaactttt cagctacttg tctatcttca atttcttttg aaatgcgtag agaattattt
     3361 tgcatatcgt gttcaaacaa ggtgtaagca ttcgtttcat ttaaagatgc taagagatta
     3421 attttatatt tctcttgttt gatataagta taattccaag aatgaataca acctttatgt
     3481 tttttataat tgacaaaatt ataatcacca taagtataag ccaatccttt tataaacttc
     3541 tttgctggct tgataatatc atctttataa tatactctgc tttcagtcca agattgaaat
     3601 ccattggcaa aaacaccaaa agtatttgca taatcagtat caatatctat cgaaaaatga
     3661 accaatcgaa tagcttgttt aggcatcaac tcaacagtat aaatagttgc atttttatct
     3721 tcatcagctt ctaataaaaa ttctaaaaca aaatcttcat gctcaaattg agaaaaatct
     3781 tgattttgga tactaatttg cttatcctct ttataataaa tcagcgaaaa ttgtttaaac
     3841 ttaataatca tcttacttaa aatgataaaa tcaattaatt gtttggtaag ataagtcttt
     3901 tatcattcaa ttttaatagc ttaaattaat tatccgtata atatttgtaa ctttcacaaa
     3961 aaaaataata tgaaaataga acagattacg gtaattggtg cgggtacaat gggcaatgga
     4021 atcgcacatg tttgtgctca attcggatat aaagttaatt tagtagatgt gagcgaaaca
     4081 gctttagaca aagctattac aacaattaca aaaaacctag acagacaatt acaaaaaggc
     4141 agtattacgg aagaaactaa aaatttaacg cttaataata ttacaataaa tacagattta
     4201 aaaacggcta aaaatagcga tattgtaatt gaagctgctt cagaaaattt taatattaaa
     4261 gagaaaatat ttcaacaatt agataccatt tgcaagcctg aaacaatttt agcaagcaat
     4321 acatcatcta tttcaattac tagaatagcc gcttgtacca acagaccaga aaaagtaata
     4381 ggtatgcact ttatgaatcc agtacctgtg atgaaattag tagagataat taaaggttat
     4441 tcaacagaca aagctacaac tgaaaccatt gttacgctta caaaacaact acaaaaaaca
     4501 cctatcgaag caaatgattt tcctggattt gttgcgaata gaattttaat gccaatgata
     4561 aatgaagcta tttatacttt aaatgaaggc gtttctggtg ttgctgaaat cgaccaaatt
     4621 atgaaattag gtatggcaca tccaatgggt ccattacaat tagcagattt cataggttta
     4681 gatgtttgtt tatcaatctt aaatgtttta tatgaaggat ttggcttgcc aaaatatgca
     4741 ccttgcccac ttttggtcaa tatggtaact gcaaaacatt taggcgtaaa aactggcaaa
     4801 ggattttacg attattctgg aggaacaaaa gaattggtcg taagtagtaa tttttcctaa
     4861 aatttagcaa aacatttatt atatttaatt gttcgtatta taacacacaa aaatttttat
     4921 tatgagtaat atcttagttc ctttagactt ctcgcccaat gcgattaatg ctttgaaata
     4981 tgcagataat ttagctaaaa gtattgatgc taatcttata gttttaaata tttatgatgg
     5041 aggcattagc aactatgagg caggttggcc aatcgaagaa ttatttgaca atgttgttaa
     5101 gatgatacaa gaaaaaacaa atgctatcat aaaagaaaat tgtagttcaa caaatatttc
     5161 tactgttttt aaaaatggta atgtaaagga tttaatcctt gaaagtatag atttattggg
     5221 agcaaatttt gttgttatgg gaacacatgg tgcttcattg attacaaaag tattatttgg
     5281 tagcaataca gcacatacca ttgcacattc taaagttcca gttttggcag ttcctagtga
     5341 tttttcattt acaccgcttc aaaaaattgt atatgcttct gatttcaata atttgaatgc
     5401 agaattgaag ctcttaaata aagtatcgga gcaattaaaa aatatcccaa ttgaaacttt
     5461 acatttaaga tacgaaactg aaagtgctga aaatatttca gaaaatttca aaaaggtaat
     5521 ttctgaaaat aattatcaaa acatacaatt acaacaagaa acttttgatt tggataatac
     5581 gctagcaaat gcgattcgtg aatatgccga ggctcaacaa aacattattc tagcgatgtt
     5641 tgctgaagat agaaatattt tccaatcatt atttttagga agcaaaacag agtatttaac
     5701 ttacaagctg aatgtaccct tgttggcaat gaataaagca aatcttagct aataggttca
     5761 ctgccagact gatgttatct ctgctccaga taggtgttat tacctgtcag caaagacatt
     5821 ggtattgttg gttgcaaacc aacaaaggtg aaagaatttt aggcaaaagt tgaaaatacc
     5881 tgagagtaaa taaagccttg ctaataacca tatttttctt tccacctttc ctttaggaat
     5941 cgccttaatt cattttcttt agcattaatt cctggttcat aaaatttagt attggaaatt
     6001 tgttctggca aaaactctaa atctataaaa ttattttcat actgatgggc atatttatag
     6061 cccttgccat aattcaaatt ttccatcaac tttgttgggg catttcttag atgcaatggc
     6121 acagataaat gattagtttt ttcagctaaa gcaattgcat cattgatggc taaataacta
     6181 gcattgcttt ttacagaagt tgccaaatag acaacacact gacttaaaat aattcgagat
     6241 tcaggagagc caatttttga aacagcatca aatgtagcgt tggctaataa taatgcattg
     6301 ggattggcat ttccaatatc ttcagatgca aaaataagca ttcgtctagc aataaattta
     6361 ggatcttcgc cagcaacaat cattcgtgcc aaccaatata cagcagcatt gacatcactt
     6421 cctcgcatgg attttataaa tgctgaaata atatcataat gttgctcccc ttttttatca
     6481 tatcgagcaa tttgtgcttg aataatggcc tcaacagttg cattttgaat agtgtttatc
     6541 tctaaattat ctgctacaat ttctaagaca ttcaacaatt tccgagcatc accacctgaa
     6601 aaataaatca aagcttcata ttcagctact tgaatatttt tagctaccaa attggcatct
     6661 tcttttagag ctttatctac taaatgcttt aattcgtctg cttctaatga gtttaaaata
     6721 tacacttgac atctggacaa caaagctgca tttacttcaa atgaaggatt ttctgtcgtt
     6781 gctccaatta atataatggt gccattttct actgcaccca ataaggcatc ttgttgtgat
     6841 ttattaaatc gatgaatttc atcaataaat aaaacttttg tgccaaaggt tcttgcttcg
     6901 tcaataattg ctcttaattc tttaacacca gcagcaatag cacttaattg ttcaaagggc
     6961 aattttgcat tttctgccaa tagtcttgct aaggtcgttt tgcctacacc aggaggtccc
     7021 caaagtataa gcgaatgtaa agccccattt tcaattagtt ttctaaatgg cttgccttta
     7081 cctaataaat gctgctgacc aatcaggtct tcaatctgct ttggacgcat tctttcggct
     7141 aaagtcaaat tcattttaaa ctaatttatt ttttagtaca acagtatcta ccattttcat
     7201 ttttccagct ctcaccaaac gcaattctct acgttctaaa gcattttcat acaattcttt
     7261 ttcgcgttcc gtttctggct caacttgctc tacttttaca ggcgttccat catcatttaa
     7321 agcaacaaat gtaaaataag cttcatgcga ttttagttct tcgttgtttc taatattttt
     7381 aataaacacc tcacaaatta cttccattga tgtattgaaa gagcgagtaa caaaagagcg
     7441 tagaattaca atatcgccaa ctttaattgg caatttaaaa gacacatcat ctaaagaggc
     7501 agtaaccgca atatgtctag acattttaat tgcactaatt ccagagcaaa catccatcca
     7561 acacaacagg tttccaccca tcaaattccc aataggattg gtatgtccgg gcataattaa
     7621 ttccgtttgt acattagctg tatccttaca ttttctaatc gtttccatag tataaatgta
     7681 ggaatattaa atgagttttt gtatctttac acactaatta caatatcaat aaattgtaaa
     7741 aaggctgcgt agttcaactg gatagaatat cagatttcgg ctctgagggt tgagggttcg
     7801 aatccttccg cggtcaccaa ttttcttcta taatgcttct aattccttct ccattagaaa
     7861 ggatattact accttttaaa aacaatatca cattgtatat caaacgagat gatttaattc
     7921 attcacatat ttcaggcaac aagtggcgaa aattaaaata caatatagaa gcctttcaac
     7981 aacaaaataa aaaaatatta gtaaccgttg gtggtgcttt ttccaatcat attatggcga
     8041 ccgctgctgt ttcaaattgg aaaaaaattc cttgtattgg tattattcga ggcgatgaat
     8101 taaatataca ttctaataaa aatttagcgt atgctcatca acaaaatatg catttagaat
     8161 ttatagatag acatttttac gcaaaaagat atgatgatga tttcttagaa ttattgtcta
     8221 acaaattgga tattcccaat caagatattt ttttcattcc agaaggtggt gcaaacatgg
     8281 aaggtgcaaa agggtgtgag gatatcgttc acgaaattga cctaaatttc gattatattc
     8341 taacagattg tggaactgga actactctcg ctggaattgc aaatgcagta catgaaaatc
     8401 aaaaagcgat aggtatttct gtgattggta acaattttga cattgaacaa tcgacacgtc
     8461 atttcacaaa caaacctttt acaatgttgc atcagtttaa ttttggagga tatgcaaaaa
     8521 ccaacactga acttcttgat tttattaagt atttctatag aatgtttcaa ataaaattgg
     8581 actatgttta tactgcaaag ttattttatg ctatttttaa tttaatagag caagattatt
     8641 ttccacccaa ttctacaatt attggcgttc atacaggtgg tgttttaaat gcagatattt
     8701 tcgatgaaat ttaataccat tcatagtata gaatccgtta tcatcaaaga taaacaatct
     8761 aaatttattg gatacgccta ccctatttat tctgaattag aagccaagga aaagatagaa
     8821 gccatcaagg ctcaacatcc aaaagcaacg catcattgtt atgcttataa attaggcttt
     8881 gataataata attatagagc aaatgatgat ggcgaaccca acggtacagc tggaaaacct
     8941 attttaaatc aaatcaatag tttaggctta accaattgtt tggtaattgt agttcgatat
     9001 tttggtggca caaaattagg cgtttctgga ttaattgatg cttataaact atgtgcaaaa
     9061 gaaaccctag cagcgacgac tatagatgaa agagaaatca taagtacttt taactttgaa
     9121 gttgcttttg agctgactaa tcaagtcttt gattatctaa acaaaaaaca atatacctat
     9181 actaagactt ttactaaaag taatattcag tttaatgttc agattactaa agaacttgaa
     9241 gatactttaa ttaacttttt agctcaaaag cagattgaat tttcagtaga ataaatttta
     9301 aggattcaaa aaatcacctg aaagtacttt ttcagcacca ttaggcaaat caaaattata
     9361 aaaatacacc cacgcttcta tttcatttaa ttcggttcta atttttactt tttctcttcg
     9421 atattcataa ggaggatttc caattccaat accttcatat tcatcaattt tcaaaagaac
     9481 atcatcgcta tttacattaa aaacatcacc ataaacgatg tcttcactat tgttagtttt
     9541 aattaatgca ggataccaat caatcttaaa caattcagca tttttaagct ctccaagacc
     9601 aactaaaaca ctattttttg ctaaataacc agccattgga tggctttgtt ccattctcaa
     9661 agtgccataa gaaaataaat aatcagacat gttgtaaaga taatcatatt catataaaaa
     9721 aagatatgtt atatgctaga taattttttg acttttttca aaaattaata tcaaaataaa
     9781 tgcttatttt tagacacatt tttaaaatat gcaaactaca atcataagaa acggaattta
     9841 ttttgacgga actggtgcaa aaggagtaaa aaaagacatc attatcaaag ataataaaat
     9901 tgatgctgtt atggaaaatg caccaaatat tgaaggtgca aaagaagttg atgcagatgg
     9961 acattgggtt tgtcctggtt ttattgatat tcatacacat tatgatgcag aattagaagt
    10021 actgccgtct ttagatgaat cagtaagaca cggtgtaacc actgtcatta ttggtaactg
    10081 ttcactttct gctgctttgg gaaaagacaa agagattgta gatttatttg caagagtaga
    10141 aaacattcct gctaaagtat taagcaaatg gattataggt aatattaaat ggtctaatgt
    10201 tcgagaatat tatgaacatt tagaaagttt gcctataggt ccaaatgtag caagttttat
    10261 tgggcactcc aatatccgaa ttgatgcaat gggtttagat ggtgcattta tgcgaccaaa
    10321 agcaacagat gctgagcttt tcaaaatgga aggcattgta gctgaagcca tgcaagctgg
    10381 ttatttaggt atgtcaattg atatgttacc atttcatcgt tgggctggcg aatatgagaa
    10441 aaagtattta ggtatttctg taccttctca aagagctgca aaatctgaat atagaagatt
    10501 ggcacacgtt ttaagaaacc acaacagagt attacaaata acaccaaacg cagttgataa
    10561 aacatcgttt gctactattt tgagattatc acatggtgga ttttttaaac caagtttaaa
    10621 aacatcaatt gttgcagcat tagatttaaa atctaatgaa aatatttata aagtattaaa
    10681 gttttatggt gctttaagca ataatgtaat gcgtgcaaat atgaaattcc aaaccttagc
    10741 ggagccattt ctaaactatg gagatggtcc aataacacct ttatttgaag agtttccatc
    10801 tatgataaag gcgataagta gctcagaagc tgaacgtaaa aaaatgtttt tagatgctaa
    10861 ttttagaaaa gtttttacaa aagaatggaa ccataaagcc acatcagttt tccctagagc
    10921 tttgaaagaa atgtgggtcg tgaatgctcc agaccaatct ttgattggca aaaattttga
    10981 acaaatcgct attgaagcag gtaaagatcc tgtgaatcat tttatggatt tattagctga
    11041 atatgatact aaattaagat ggaaatgtga tacttctaat catagagaaa aaatccgttt
    11101 agatttatta aatagtgaac attgtatgcc aggattcaat gatagcggtg cacacaatgt
    11161 aaatatggct ttccatgatg gcagtttaca aatgctaaaa tctgtacaaa aacatcctga
    11221 agtaatgagc atagaaagag ctatttatag attaacaaaa gaagctgctg actttttagg
    11281 tatagatgct ggcgatattt cggtgggcaa acgtgcagat gtggttatta tcgatcctag
    11341 taaattagat tcagatataa attctagtcc aattgaagat ttccatccag ctttagatgg
    11401 tgcatacaga ttggtaaaac gtagtggaaa ggttgttaaa catgttttca ttaatggtat
    11461 tgaggcttat agcaacaatg gtgtcgttgg atttcatgaa ggtttaggaa aaactaaaca
    11521 atttggtcaa ttactgaaaa gtacttggag atagttcaat ttaataccga aacagaacaa
    11581 aagttttatt caatttctga attaataatg tagtttgttc attattaaat cagaaaatga
    11641 ataatttgaa ttacattact atttcttttt aaactattaa aagctaataa aattctacct
    11701 tcaaaagtat ttagaatttt caaaagtagt ccaatacact tttaggctta cagataagct
    11761 tctaatttat attaaagatt aactaacttt atggtctatt tttatgactc aatcagtaat
    11821 taacagaaag tttctaatta tactattagg tattttggct gcacttggac ctttctcgat
    11881 tgatatgtat cttccaggtt ttcaacaaat agctgaagaa tttaatgtcc atgaaaaagc
    11941 agttgcattc acacttacct cctatttttc tggcatagct gttggtcaat tactatatgg
    12001 tccaatcgtt gataaatatg gtcgaaaaat accattatta gtcggtttat ctatatacat
    12061 gttagcttca ttaggttgtg ctttagcaag cagtattgag tttttaatag ccatgcgatt
    12121 attgcaagct ctaggcggtt gtgttggcat ggtcgcatct aatgcaatta ttagtgatgt
    12181 ttatgaaaaa aatgaaagag caaaagcatt ttcttatatt atattaataa tgggattggc
    12241 tccgctaatt gctccaagtt taggtagctt ttttgttgca cactttacgt ggcattatat
    12301 tttttatttc ttatttattt tctcactttc ggtaattctt ttaatttcct ttttcttacc
    12361 tgaaactagt atttataagc attctaacaa attaaaattt ataaagattt caaaagacta
    12421 tcttgaagtg ctaaaaaatc ctatattttt aaaatatacc ttagcaagta gcattgtaat
    12481 gtctattcta tttgcttata tatcttcagc tccattttta tttatgacct tatataaatt
    12541 ggacaatact acattttcaa tcttatttgc tgtaaatgct gcaggcttta tagctggcag
    12601 ttattgtaat ggcgttttaa cgaaatacat tccctatatc aaaattgcaa aagtagcctc
    12661 tgtaatatta gcatcaattg cattagcagc cacaattact tattatatta atcctcaaat
    12721 tccttataaa atattaacta gtggtatatt ttttattctc gtattaattg ggtttattaa
    12781 tcccaatgca actgctggtt cacttgcacc atttacaaat aatattggaa tagcttcagc
    12841 attaggtggt tcatttagaa tgggaattgg tgcaattgta gctgcattta ttggaagttt
    12901 tcaaggcagc acagctatta ttttatttgt aattattttt atattgtcaa ttgctacagt
    12961 ttcactttta tggataaaaa caaagaaaat caatcattaa taaagtaaaa taaagatatt
    13021 aggatacaaa aaaataaata tacgacatct tatatgtcca aaatatccta tttaacaaca
    13081 aataagaagt atctcatcta aaattagtaa ttttgttagc tattatagtt atgagtaatt
    13141 taattgataa atacaatata cctgggccac gatatacatc ttatccaact gttccttatt
    13201 gggataacaa tactttttcg tttaataatt gggaaatttc agttttaaaa tcttatgaag
    13261 aaagtaatga ttctgaaggt atttcaattt acatgcacct tccattttgt gagcaattat
    13321 gtaccttttg tgcttgccat aaacgaatca caaaaagaca cgaagtggaa acaccttatt
    13381 tggaaagtgt tataaaagaa tgggaacttt atttacaact atttaataaa aataaaaaaa
    13441 caacgccaaa gctcaaagaa ttgcatttgg gcggaggaac tccaacattt ttttcaccag
    13501 aaaacttgaa aattttatta gatagcttat tttctactgt agagattgct gaaagctctg
    13561 aattttcatt tgagggacat cctaacaata caactaaaaa acatttggaa gtattatata
    13621 atttaggatt tagacgttgt agttttggtg ttcaagacta tgatttaaaa gtgcaaaaag
    13681 ccattaatag aattcaacct tttgaaaatg tacaaaaagt aactgattgg gctagagcaa
    13741 tcggttataa aagcatatcg cacgatttgg tttttggttt gccacaccaa acttgggaat
    13801 cagttgaagc aactattcgg aaaactttgg agctaaaacc agacagactt gctttctatt
    13861 cgtatgcaca cgtaccttgg gtaaaaggtg ttgggcaacg tgggtatgat gaaaatgact
    13921 taccaagtgg cgaaaccaaa cgaaaattgt atgaaaacgg caaacaatta ttacaagatt
    13981 taggctatat cgaaattgga atggatcatt ttgcattaga acatgatgat ttatatcaat
    14041 cattaataca aaataaatta caccgtaatt tcatgggtta ttcctctgga aaaacacagt
    14101 taatgattgg cttaggtatg agtgccattt cagattcttg gtatggattt gcccaaaaca
    14161 ataaaacggt tgaagattac caagcaatag ttgaaagtgg tcaaattcct gtaaatcatg
    14221 gacatatttt aactgaagaa gatttaatta tcagaaaaca tattttgaat ttaatgtgtc
    14281 aattagagac taattttaat aataaaaata gctttcctga actgcctgaa gttttggata
    14341 aattaaaaga aatgcagaaa gatggcttgg ttgaaattag ttcaaaaaca gtaaaaatta
    14401 ccgaaaaagg aagagcattt tcgagaaacg ttgcaatggc atttgattta agaatgatac
    14461 gcaaacagcc agaagcaaga atattttcaa tgacaattta attttttatg aaagaagaat
    14521 ttatttacac accaataaat ataaaccatg ctgaaaatcg atttgaaatg atggttgatg
    14581 gttatatggc ttatattgaa tttgaaattc acaatggaat ttatagttta acacacacaa
    14641 ttagtccaaa agaaattgga ggcagaggtg ttgcaagttc attaattgag aaggtactta
    14701 tatacctaaa tgctgaaaaa cggcaattta taccaatatg cccaatggtt ataaatttta
    14761 tacaaaaaca ccctgaatgg aaaaaatgga ttcctaagca ataccacgaa gagattgaca
    14821 aagcttaata gaatatttat tatcctttaa aagtgatatg agataggatt tttctaaatt
    14881 aaccattcaa aactcaatta tacttttatt caattcactt ttgctaaata atggaactat
    14941 aattagatat aataacctaa tatagattaa aaacattaaa ttagtcaaat ctaattcaca
    15001 taatatgtca gaaacaacac aagattatca tattatagaa aagtttcgtg aaatgattct
    15061 caaaagatat gaatatgctg atttaaaaaa cagatttgaa ctatcagact ccataacaga
    15121 agaagttgtt gcagaggtaa aaaattattt tttaacgaca atatatccgc ctgcacaaga
    15181 aaggaaagaa cttgaagatg cctttcgaga tttggcagct tatgtaaaac agccacgtaa
    15241 aatttggaat ttatttggag atatggcaag agctattttt aagtttggta ggcattttat
    15301 gtctgcttta aaagctggaa tggactcttt agattcattt atgggagcca aagactttga
    15361 agctgctatg acagaaattg ctaataaaaa tggcatgaaa ccaccaatgt ctgatgaaga
    15421 ttatgaagat gccatgtatc aattagatag agttaaaa
//
LOCUS       DAOQCW010000057        15409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1392_length_15409_cov_5.902110, whole genome shotgun sequence.
ACCESSION   DAOQCW010000057 DAOQCW010000000
VERSION     DAOQCW010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15409
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1392_length_15409_cov_5.902110"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="PLS16_06500"
     CDS             complement(<1..585)
                     /locus_tag="PLS16_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNJ60765.1"
                     /translation="MAHQQFEKLTDIEVIHRILNGEKPLYELIVRRYNPYLYKIGRSY
                     NYNHDDTQDLMQDTYVDAFRNLSQFESRAGFKTWIARIMLNNCFRKKEKLSFKNEYAE
                     DNINEKSIPMFTSNSNDTSHIINNKELGGIIEKALEVIPEDYRMVFSLREINGMNVLE
                     TANLLGISEANVKVRLSRAKEMLRNILENNYEPKE"
     gene            complement(620..1000)
                     /locus_tag="PLS16_06505"
     CDS             complement(620..1000)
                     /locus_tag="PLS16_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60766.1"
                     /translation="METQNQQQIVKNVFNLLKITFVLIPIVAGADKFTNLLTNWEQYL
                     NPTLAGFLPFSGAVFMMIAGVIEIIAGLIVLKKPEIGGLIVAAWLVCIALSLLVGFNY
                     VDVAVRDIVMAIAAFSMAKLAKAV"
     gene            1252..3834
                     /locus_tag="PLS16_06510"
     CDS             1252..3834
                     /locus_tag="PLS16_06510"
                     /inference="COORDINATES: protein motif:HMM:NF009397.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HNJ60767.1"
                     /translation="MGEQNEQHITTLDGLYENWFLDYASYVILERAVPALYDGLKPVQ
                     RRIMHTMKTLDDGRFNKVANIVGSTMQYHPHGDASINEAIVNIGQKDLLIDCQGNWGD
                     IRTGDSAAAARYIEARLSKFALEVMFNEDTTEWQLSYDGRKKEPVLLPVKFPLLLAQG
                     IEGIAVGLSTKVMPHNFCELIDASIKYLKKEDFILYPDFLTGGLVDVREYNDGKRGGK
                     IRVRAKVETIDKKSLRISEIPFGTTTANLIDSILKANEKGKIKIKKVHDNTAKDVDIQ
                     IELQPNISTSVAIDALYAFTDCELSISPNTCVIIDEKPHFTDVSSALKISTDTTVQLL
                     KRELEILLGELQDKWHFSSLEKIFIENRIYRDIEEEETWEGVISAIDKGLDPFKNLLQ
                     RAVTQDDIVRLTEIKIKRISKFDAKKADEYIKSLEDEMAQTQKNLKGLTAFAIKYFEN
                     LLKKYGKGRERKTEITNFDTVEVKLVAANNEKLYVNRAEGFVGYGKDLKKEEFISECS
                     DIDDIIAFTKDGIMKVVRNSDKVFIGKDIIHVAVWKKNDERTTYNAVYLDGKSGNNYV
                     KRFNVTSITRDKEYPITQGNPKSKLLYFTANPNAEAEIIDVQLTQGCSARIKRFDYDF
                     SELTIKNRSSQGNILTKYPVRKIELRAKGQSTLGGLDIWLDDDIGKLNTEQRGKYLGS
                     FQNDDKILVLLKSGEYMLTNFELTNRYNMNDSISIEKYYPNTIISAIHYIPSKKCNYI
                     KRFQIETSTLEQRFSFIADEKDAQLVFGTTVPNPTIEYTLDIGKNKSSEPEQSVLASV
                     IEVKGWKAVGNKFVGGTIKSIALIESKLVEESTETKETINQNIDFEITNLKENTEGEQ
                     GELF"
     gene            complement(3859..4764)
                     /gene="hemF"
                     /locus_tag="PLS16_06515"
     CDS             complement(3859..4764)
                     /gene="hemF"
                     /locus_tag="PLS16_06515"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HNJ60768.1"
                     /translation="MTSLDSIKNYLVDLQQQICNGLEKLDGKAVFVSDKWERTEGGGG
                     DSRVIIDGNVFEKGGVNFSHVHGKLPEVLKSETRHADYFHATGVSIVIHTQNPYVPII
                     HMNVRYFCMMEAEDGTIVDEWFGGGIDLSPAYPNEQDIIFFHQYLKNECDKFDSDYYP
                     KFKTWCDEYFTIKHRNQMRGIGGIFYDHLRPNDTCNKQELFEFMQAVGNSFLPIYTEI
                     VNRNKQRTFTDAQKEWQFIRRGYYTEFNLVYDRGTHFGLKTNGRIESILMSLPPHAEW
                     KYNFQPEINSEEYKALQYFQAKNWV"
     gene            complement(4865..6265)
                     /gene="fumC"
                     /locus_tag="PLS16_06520"
     CDS             complement(4865..6265)
                     /gene="fumC"
                     /locus_tag="PLS16_06520"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HNJ60769.1"
                     /translation="MEFRIEKDTMGQVKVPAHVYWGAQTQRSIENFQIAQDINKMPKE
                     IIRAFAYLKKAAAITNHKAKVLDKKKSDIIGQVCDEILAGKLDDQFPLVVWQTGSGTQ
                     SNMNCNEVIAYRGHVLLGGNLADEKKLINPNDDVNKSQSSNDTFPTAMHIAAYTILVE
                     KTIPGIKKLRNTLAKKSKAFANVVKIGRTHFMDATPLTLGQEFSGYVSQLDHGIKAIE
                     NTLAHLSELALGGTAVGTGINTPKGYAKNVAKEIATLTGLPFITADNKFEALAAHDAI
                     VEAHGALKTVAVSMMKIANDIRMLSSGPRCGIGEIHIPDNEPGSSIMPGKVNPTQCEA
                     MTMSAAQIMGNDVAINIGGSNGHFELNVFKPVMIYNFLHSARLIADVCVSFNDKCAVG
                     IEPLHDNIKRNLDNSLMLVTALNPKIGYYKAAEIAQTAHKNNSTLKETAIKLGYLTAK
                     EFDEWVRPEDMIGGLK"
     gene            complement(6372..6974)
                     /locus_tag="PLS16_06525"
     CDS             complement(6372..6974)
                     /locus_tag="PLS16_06525"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNJ60770.1"
                     /translation="MEVALKGISIGVMVSFLIGPIFLGLIDITITRGWKSGLAYILAI
                     IVSDAALIILINEIFTEIHIEKFKFYVGLIGGIILMLFGVITFISKTNLKGVDTENVK
                     TIGGAFLKGVSINLTNPFVIVWWMGLYTATAVQGYTSNEKFIYYFFILFTIFFFDLLK
                     MRFGYYLKYNLSVEKLSMFKKLVGISLFIFGIVMMLRVLL"
     gene            7093..8319
                     /locus_tag="PLS16_06530"
     CDS             7093..8319
                     /locus_tag="PLS16_06530"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNJ60771.1"
                     /translation="MIENEFDILIVGGGASGFFTAIQIAEQNPNLSICILEQSKKVLE
                     KVKISGGGRCNVTNICSDPKELVKNYPRGNKNLLNIFYAFNTTHTIRWFEGHNIQLKT
                     ESDGRVFPKSNTSQTIIDCFLNLCHQYKITIQTEQKVVQIKAIEQAFHLQTLNKKYIC
                     KKLVLACSTNKIILDEIEKLNISTIPLIPSLFSFNTKNLLFKELAGITLENCTAQIVN
                     TKIRTEGNILITHTGVTGPCILKLSAYGAKELFQLNYNTDIIINWNDDNFNDTISVLS
                     DLKISEAKKKVSNVKPYSIPNRFWNNLLAISSIDKEKKWADLNKQEIKNLAINLSQFS
                     LRINGKSTNKDEFVTAGGISLQAVNMKTMQSKQYENLYFTGEILDIDGITGGFNFQAA
                     WSTAYLAAKHIASLDS"
     gene            8493..9008
                     /locus_tag="PLS16_06535"
     CDS             8493..9008
                     /locus_tag="PLS16_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNJ60772.1"
                     /translation="MEIPASAQKILNAETAALKVDRIVFQILENTFHSPEIVMIGIKD
                     GGYIIAEKIAEKINHYYPEKKIALLSLTIDKINPVGNTILLEPNSNLEGKTIVLIDDV
                     ANTGKTMFYTFNELTKYKIEKLITCVLIDRTHKKFPIQSDIVGLQMATTFNEHIIVKI
                     ENNEIEGAYLF"
     gene            9025..10128
                     /locus_tag="PLS16_06540"
     CDS             9025..10128
                     /locus_tag="PLS16_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60773.1"
                     /translation="MKKEVIFAFLLFISTFCFSQKNGFNIDDLVEPNEQELLIIERIN
                     YLNEMRKLSSNLFWKDFGLDEFPGTIVYFTDSLSYFINPEQSVLDKISKFKALKNPYD
                     YTILRLALPFDAPTFVIQTLFETSEGNKKNINYMLPILFCSSPEVSKTLQPEINSTEL
                     WASNTLHEYFHQYQFKIKAIYSYLNVFISQKKMFDKDSMQSIYNKNWNFKDTLVKEND
                     LLLKAINSNSLEAEKNYFTQFLKSRNKRRLEYQKKTKFAIGQIEDLWEKIEGTTLYLE
                     AVLKQNFGQLPPQHYLIQHDTLFLNQNIFSGFNLATDLKYTSIQDADYYFGATGVNLI
                     RLLEKHNVDYKKDFFQYASMPLSTQLKYFYKIK"
     gene            10133..10792
                     /locus_tag="PLS16_06545"
     CDS             10133..10792
                     /locus_tag="PLS16_06545"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HNJ60774.1"
                     /translation="MFSKLFSKKTIILLCTFVFLITISAIVFCNNKVEQFSNDKLYND
                     INKIPHNHVGLLLGTCKTLKDRITINPFWQHRLEATYLLWKHKKIDIILISGDGGWHG
                     YNEPEDFRKAFLKLGVPDSVIVIDAAGFRTHDSVIRALKIFGLKKFTIISQLFHNKRA
                     LVIANKYNIDAIGFNANDIGRKHAWFNYFREKLARVKLFLDLYILNTQPHLLDDTRFI
                     N"
     gene            10804..12303
                     /gene="glpK"
                     /locus_tag="PLS16_06550"
     CDS             10804..12303
                     /gene="glpK"
                     /locus_tag="PLS16_06550"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HNJ60775.1"
                     /translation="MKMKYILALDQGTTSSRAILFDESSNIVAVAQKEFTQIFPQPGW
                     VEHNPEEIWQTQLETAKEVIVKANILPSNIAAIGITNQRETTVIWDKETGKPIYNAIV
                     WQDRRTASFCDELKARDLQKYVGENTGLVIDAYFSGTKINWILNHVEGAREKAHQGKL
                     LFGTIDTWLIWNLTKGTVHATDYTNASRTMIYNIKNLEWDKKLCLDLDVPTSILPQVL
                     NSSDDFGKTHTDILFNESIPITGVAGDQQSALFGQACFEKGMAKNTYGTGCFMLMNTG
                     EKFVQSKSGLITTIAWGLDGKITYALEGSVFVAGAAIQWLRDGLKLIDTAKDSEYFAL
                     KVDDTGGVYVVPAFVGLGAPYWNMYARGAIFGLTRGTGKAHLIRATLESLAYQTNDIL
                     QAMQQDSGINLSELRVDGGACANNLLMQFQADIIDVPVERPKLIESTALGAAFFAGIY
                     VGLYSIEKINKIKAIDHRFEPKMQEEKRTKLLKGWKKAIKRAIDWELDE"
     gene            12412..12609
                     /locus_tag="PLS16_06555"
     CDS             12412..12609
                     /locus_tag="PLS16_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60776.1"
                     /translation="MKKLFVLFAAATLFVGVQACKSTEQPAEEVVEATEEVVEDAAAV
                     VDSAAAVVDSAATVVTETVAK"
     gene            12731..12925
                     /locus_tag="PLS16_06560"
     CDS             12731..12925
                     /locus_tag="PLS16_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60777.1"
                     /translation="MKKLFVLFAAATLFVGVQACKSTEQPAEEVAATEEVVEEAPAVV
                     DSAAAVVDSAATVVTETVAK"
     gene            13152..>15409
                     /locus_tag="PLS16_06565"
     CDS             13152..>15409
                     /locus_tag="PLS16_06565"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HNJ60778.1"
                     /translation="MRIFLFVFLICNVLFVKAQYFIDNAVAPQSHLALWYKQPAENWM
                     TSALPIGNGRIGAMVFGGVIQEEIQFNDKTLWTGNKNTRGSYQNFGNILLNFPKLDTI
                     EHYRRILDLETATCQIDYKARNINYKRTYFASYPDNAIIMQFSSDTEAAISFDILLND
                     AHSSEIKILDNSMVFRGKLDILSYEAQLSIKHEGGILKKDNHQLSIQNANTVTLILTL
                     GTNYNPVEADYIGYDATELSSIISNQMSSAYSKTYDTLFARHVSDYKSLFDRVHLNLG
                     NTNPSIPTDQLQLLYNKGDRNTALDVLYFQYGRYLMISSARGIAMPSNLQGLWNDSNL
                     PAWGSDIHTNINVQMNYWPAEITNLSELHSSLTDYVFNEAVLQPAWQENATTLGAKGW
                     TLRTQCNIFGYSDWKWNRPANAWLAMHLWQHYTYTLDTVYLQQKAYPVMKSACDFWLS
                     YLIKDKNGKLVAPKEWSPEHGPWQDGVAYAQQLIFNLFENTLKANEILGENNSYHITL
                     KNTLLNLDNGIAIGHWGQIKEWKNYKRGENKKKKQHRHISHLIALYPGNKISPLLDKK
                     YSDAAIKSLNQRGDSGPGWSRAWKINAWARLLDGERAYQLIQQALQNTTSASTNFDEN
                     GGAYQNLLDAHPPFQIDGNFGATSGIAEMLLQSHLEKIQLLPAVPKAWSKGSVYGLKA
                     MNNFTVDIIWDLDLQTQAIIHSGSGKICSIYFKNIANAKITDNNGRPVPFIVINNDEI
                     KFPTSKGLSYTI"
BASE COUNT         5615 a         2307 c         2415 g         5072 t
ORIGIN      
        1 ctctttaggt tcataattat tttcaagtat atttctcaac atttcctttg ctctactcaa
       61 tcttactttt acattggctt cgctaattcc tagtaagttg gcagtttcca acacattcat
      121 tccgttgatc tccctgagtg agaaaaccat tctataatct tcaggaatta cttcgagtgc
      181 tttttctata ataccgccta attctttatt attgatgata tggcttgtat cattgctatt
      241 gcttgtaaac ataggtattg atttttcgtt gatattatct tctgcatatt cgtttttaaa
      301 acttaatttt tctttttttc taaagcaatt attcaacata attcgagcaa tccaagtttt
      361 aaaaccagca cgactttcaa attggcttaa atttctaaag gcatcaacat aagtatcttg
      421 cattaaatct tgcgtatcat catggttgta attgtaagaa cggccaatct tgtataaata
      481 tggattatat cgtcttacaa tgagttcata aagtggtttt tcgccattta agattctatg
      541 aattacttct atatctgtta atttttcaaa ttgttgatgt gccatgagtt ttcattttta
      601 ttatacaata ataaacattc tacactgcct ttgccaattt tgccattgaa aatgcagcaa
      661 ttgccatgac aatatctcta actgcaacat cgacataatt aaatccaact aaaagcgata
      721 aagctataca cactaaccac gcagcaacaa ttaagcctcc aatttctggc ttttttaaga
      781 caattagtcc agcaataatt tcgataactc ccgcaatcat cataaatact gcaccagaaa
      841 aaggcaaaaa ccctgccaaa gttgggttga ggtattgttc ccaattggtc aataaattag
      901 taaatttatc cgctcctgcg acaattggaa ttaagacgaa agtaattttc aataagttaa
      961 atacattttt tacgatttgt tgttgatttt gtgtttccat tatattctaa attttgttgt
     1021 taataaatat tggttctata taaatagagt caatgaaaca agaaaaggtt acaagcaaaa
     1081 cgaaaaaaat taagtacaaa atcaaagaca ttgattatca attcattaaa attaattatt
     1141 taatgaaatt atttttactc aagaaataat ctacactcgt ttacaaatat ttctttgcct
     1201 tcaaaataga taaaagtata tttacgctga attttacaga agattgaatt tatgggagaa
     1261 caaaatgaac aacatattac aactttagat ggcttgtatg agaattggtt tttagattat
     1321 gcctcttatg taatattaga acgtgcagtt cctgctttgt atgatggatt aaaaccagtg
     1381 caacgtagaa ttatgcatac gatgaaaaca ttagacgatg gtcgttttaa taaagttgcc
     1441 aatattgttg gaagtacgat gcaatatcat ccacacggcg atgcttctat taatgaagcc
     1501 attgtaaata taggtcagaa agatttattg attgattgtc aaggaaactg gggagatatt
     1561 agaactggag atagtgctgc tgctgcgaga tatattgaag ctcgtttgtc aaagtttgcc
     1621 ttagaagtga tgtttaatga agataccaca gaatggcaac tttcgtatga tggtcgaaaa
     1681 aaagaacctg ttttattgcc tgtaaaattt ccattattat tagctcaagg tattgaaggt
     1741 attgcagttg gtttatctac caaagtaatg cctcacaatt tttgtgaatt gattgatgca
     1801 tctatcaaat atttaaaaaa agaagatttt attttatatc cagacttttt aactggcggt
     1861 cttgtggacg taagagaata caatgatgga aagcgtggcg gaaaaattag agtacgtgca
     1921 aaagtagaaa caatagataa aaaatcactt agaatttcag agattccatt tggaacaaca
     1981 actgccaatt tgattgattc tatcttaaaa gccaatgaaa aaggcaagat taaaatcaaa
     2041 aaagtacatg ataatactgc aaaagatgta gatatacaaa tcgaattgca acctaatatc
     2101 tcaacaagtg ttgcgattga tgcattgtat gcttttaccg attgtgagtt gtctatttcg
     2161 ccaaatactt gtgtaattat tgatgaaaaa ccacatttta cagatgtttc atctgcttta
     2221 aaaatatcaa cagatacaac tgtacaatta ttaaaacgtg aacttgaaat tctattgggt
     2281 gaacttcaag acaaatggca tttttcatct ttggaaaaaa tatttataga aaatagaatt
     2341 tatcgagata tagaagagga agaaacttgg gaaggcgtta tttcagcaat tgacaaaggt
     2401 ttagaccctt tcaaaaattt attacaaaga gcagttactc aagatgatat tgttcgatta
     2461 acggaaatta aaattaaaag aatttctaag tttgatgcaa aaaaagctga tgagtatatt
     2521 aaatcgttgg aagatgaaat ggcacaaacc caaaagaatt taaaaggact tacagctttt
     2581 gctatcaaat attttgaaaa tttgcttaaa aaatatggca agggcaggga acgaaaaaca
     2641 gaaattacca atttcgatac tgtagaagta aaattagtag ctgccaacaa tgaaaaattg
     2701 tatgtaaaca gagccgaagg ctttgttggt tatggaaaag atttaaagaa agaggaattt
     2761 atttcagaat gttctgatat agatgatatc attgccttta ctaaagatgg tattatgaaa
     2821 gtagttcgca attcggataa agtatttatt ggaaaagata ttatccatgt tgcagtttgg
     2881 aaaaagaatg acgaaagaac aacatacaat gcggtttatt tagatggaaa atcaggcaat
     2941 aattatgtca aaagatttaa tgtaacctca attacaagag acaaggaata tccaatcact
     3001 caaggtaatc caaaatcgaa actattgtat tttactgcta atccaaatgc tgaagctgaa
     3061 attattgatg ttcaattgac acaaggttgt tctgctagaa tcaagcgttt tgattatgat
     3121 ttttcagaat taaccatcaa aaatagaagt tcgcaaggaa atattttgac caaatatcct
     3181 gttagaaaaa ttgaattaag agctaaagga caatctactt tgggtggctt agatatttgg
     3241 ttagatgatg atattggcaa acttaatacg gaacagagag gcaaatattt aggcagtttc
     3301 caaaatgatg ataaaatctt agttttatta aaatcaggag aatatatgct gactaatttt
     3361 gaattgacca atcgttataa tatgaatgat agtatttcta tcgaaaaata ttatccaaat
     3421 acaatcatat cagcgattca ttatattcca tcaaaaaaat gtaattatat caaacgtttt
     3481 cagattgaaa cttcgacatt agaacaacgt ttctcattta ttgcagatga aaaagatgct
     3541 caattagtat ttggtacgac tgtcccaaat cctacgattg aatacacttt ggacatagga
     3601 aaaaacaaat catcagagcc tgaacaaagt gttttagcat cagtcattga ggtaaaaggt
     3661 tggaaagctg tcggtaataa atttgttggt ggtacaatta aatctattgc gttaattgaa
     3721 tctaaactag ttgaagaatc aacagaaaca aaagaaacta tcaaccaaaa tattgatttt
     3781 gagattacca atctaaaaga aaatacggaa ggcgaacaag gcgaattatt ttaaagctaa
     3841 agctctaaag taatttttct aaacccaatt tttggcttga aaatattgca aagccttgta
     3901 ttcttctgaa tttatttcag gttgaaaatt atatttccat tccgcatgtg gtggcaagct
     3961 catcaatatc gattctattc ttccattagt ttttaaacca aaatgcgttc ctctgtcata
     4021 gaccaagtta aattcagtat aataacctct acgaataaat tgccattcct tttgggcatc
     4081 cgtaaaagtt ctttgtttat ttctattaac gatttcagta taaataggta aaaaactatt
     4141 tcctacagct tgcataaatt caaacaattc ttgtttgtta caagtatcat tcggacgtaa
     4201 atggtcataa aaaataccac ctataccacg catttggttt ctgtgtttga tagtaaaata
     4261 ttcatcacac caagttttaa attttggata ataatctgaa tcaaatttat cacattcatt
     4321 ctttaaatat tgatggaaaa atataatatc ttgttcattt ggataagctg gcgacaaatc
     4381 tattccaccg ccaaaccatt catctacaat ggttccatct tcggcttcca tcatacaaaa
     4441 atatcgaaca ttcatgtgaa taattggaac ataaggattt tgggtatgaa taacaataga
     4501 aacgccagta gcatgaaaat aatcagcatg tcgagtttca gattttaata cttcaggcaa
     4561 tttaccatga acatgagaaa aattaacgcc acctttttca aaaacatttc catctataat
     4621 tactctagag tctccgcctc caccttcggt tctttcccat ttatctgaaa caaatacggc
     4681 tttcccatca agtttttcta agccattaca aatttgttgc tgcaaatcaa ctaagtaatt
     4741 ttttatcgaa tctaatgatg tcatgattaa tatttatatg taaaactaag tatttattaa
     4801 aaaaaagagc ttatccaagg ttatggacaa gctcgtagaa tttaagaatt tttcaaacct
     4861 caacttattt caatccacct atcatatctt ctggacgtac ccactcatca aattcttttg
     4921 cagttaggta gcctaatttt atagctgttt cttttaaagt agaattattt ttatgtgcag
     4981 tttgagcaat ttcagccgct ttataatagc caatttttgg atttaaagca gtaactaaca
     5041 tcaaactatt atccaaattt cttttgatat tatcgtgcaa aggttcaata ccaacagcac
     5101 atttatcatt gaaagaaaca caaacatcag caattaatct cgcagaatgt aagaaattat
     5161 aaatcattac aggtttaaaa acattcaatt caaaatgtcc atttgagcca ccaatattaa
     5221 tagctacatc atttcccata atttgagctg ccgacatggt catcgcttcg cattgagtag
     5281 gatttacttt tcctggcata attgaagaac ctggttcgtt atcaggaata tgaatttcac
     5341 caataccaca tcttggtcca gaagacaaca ttctgatatc attagcaatt ttcatcattg
     5401 aaactgccac tgttttcaat gctccatgag cttccacaat cgcatcatga gccgccagag
     5461 cttcaaattt gttatctgca gtaataaatg gtaatcctgt taaagtggca atttctttag
     5521 ccacattttt tgcataacct ttaggtgtat taattcctgt accaactgct gttccaccta
     5581 aagccaattc tgataaatga gccaaagtat tttctatagc tttaattcca tgatctaatt
     5641 gagatacata acctgaaaac tcttgaccaa gtgtcaaagg tgtagcatcc ataaaatgtg
     5701 tacgaccaat tttaaccaca ttagcaaaag cttttgattt ttttgctaaa gtatttctca
     5761 gttttttaat tcctggaatt gttttctcta ccaagatagt ataggctgca atatgcatgg
     5821 ctgttggaaa agtatcattc gatgattgtg atttattgac atcatcattt ggattgataa
     5881 gttttttctc atctgcaaga tttccaccta ataaaacatg accacgatag gcaatcactt
     5941 cattacaatt catatttgat tgagttcctg agcctgtttg ccatactact aatggaaatt
     6001 ggtcgtctaa ttttcctgct agtatttcat cacaaacttg tccaataatg tctgactttt
     6061 tcttgtctaa aacttttgct ttgtgattgg taattgctgc agcttttttt aaatacgcaa
     6121 atgctctaat aatttcttta ggcattttat taatatcttg tgcaatttga aagttctcta
     6181 tagaacgttg ggtttgagca ccccaataca catgagccgg aactttaact tgtcccatgg
     6241 tatctttctc aattctgaat tccattttct aaaaattata ggttataaat acttttgtaa
     6301 ttaaaaaatc gctgtaaata tacggaatta tacaatttca ttaattataa tacttgataa
     6361 ttttgtctta tttataataa aactctgagc atcatcacaa ttccaaaaat aaataaggaa
     6421 attcctacca atttcttaaa catagaaagt ttttctacac ttaaattata tttaagatag
     6481 taaccaaaac gcatcttcaa taaatcaaaa aagaaaatgg taaataaaat aaaaaagtag
     6541 taaataaatt tttcatttga ggtataacct tgaacagcag tagcagtgta taagcccatc
     6601 caccagacaa ttacaaatgg gtttgttaaa ttgattgata cacctttaag aaatgcaccg
     6661 ccaatagttt ttacattttc agtatccacg cctttcaaat ttgttttaga aatgaatgtt
     6721 attacaccaa ataacattag gattatccca ccaattaaac caacataaaa cttaaacttt
     6781 tcgatatgaa tttcagtaaa tatttcatta atcaaaataa ttaatgctgc atcgctcaca
     6841 ataatagcta agatatatgc caaaccagat ttccagcctc ttgtaattgt aatatcaatt
     6901 aatcccaaaa aaattggacc aattaaaaaa ctaaccatta cgccgattga tattcctttt
     6961 aaagcaactt ccattattgc caaaaataca taaaatagat ataaactgat attaggatat
     7021 tactaccaaa ttcagcattt aagacaaatc gttttatagt aagtctaatt ataaaattcc
     7081 tatttttgca taatgattga aaatgaattt gatattctaa ttgtgggtgg tggagcaagt
     7141 ggctttttta cagctattca aattgcagaa caaaatccta atctttcaat ttgcattttg
     7201 gaacaaagca aaaaagtatt agaaaaagta aaaatttcag gtggcggaag atgtaacgta
     7261 acaaatatat gttcagaccc aaaagaatta gtaaaaaatt acccaagagg caataaaaat
     7321 ttattgaata tattttatgc ttttaataca acacatacaa tccgttggtt cgaaggccat
     7381 aatattcaat taaaaactga aagtgatgga agagtttttc caaaaagcaa cacctctcaa
     7441 acaataattg attgtttttt aaacttatgt catcagtata aaattacaat tcagacagag
     7501 caaaaggtag ttcaaattaa agcaattgaa caagcgtttc atttacaaac tttaaataaa
     7561 aaatacattt gcaaaaaatt agttttggcg tgtagtacca ataaaattat tttagacgaa
     7621 attgaaaaat taaatatctc tacaatacct ttaattcctt cattatttag ttttaatact
     7681 aaaaatctac tatttaaaga attggcaggc ataaccttag aaaattgtac tgcacaaatt
     7741 gtaaacacta aaattagaac tgaagggaat attttgatta cacatactgg cgttactgga
     7801 ccttgtatat tgaaattatc agcttatggt gcgaaagagc tgtttcagtt gaattacaat
     7861 acagatatta ttatcaattg gaatgacgac aattttaatg atacgatttc agtattatct
     7921 gatttaaaaa ttagtgaagc taagaaaaaa gtaagcaatg taaaaccata ttctattcct
     7981 aatagatttt ggaataattt attagctatt tcttcgatag ataaagaaaa aaaatgggca
     8041 gacttaaaca aacaagaaat aaaaaatcta gcgataaatc tttcgcaatt tagtttaaga
     8101 attaatggaa aaagcaccaa taaagatgaa tttgtaaccg caggaggcat ctcacttcaa
     8161 gccgtcaata tgaaaacaat gcaatccaaa caatatgaaa acctatattt cacaggcgaa
     8221 atattagata ttgatggaat tactggaggt tttaattttc aggcagcatg gtccactgct
     8281 tacctcgctg caaaacatat tgctagcttg gactcataga agtgattaaa aataaatgag
     8341 taacgaagaa gatgggagga attgggtttg agatttttga ataatgattt tcttgaaacg
     8401 gtcttaaatt atgaataatg gattacaaat gacaggctat atttatgaat tatttcatta
     8461 ttaaattatt ccatttttct tatatttgag gtatggaaat tcctgcttca gcacaaaaaa
     8521 tattgaatgc cgaaactgcg gctttaaaag tagatagaat cgtatttcaa atcttagaaa
     8581 atacctttca tagtccagaa attgtaatga ttgggataaa agatggtggt tatattattg
     8641 cagaaaaaat tgcagaaaaa ataaaccatt attatccaga aaaaaaaata gcattgcttt
     8701 ctttaaccat cgacaaaata aatcctgttg gaaatacgat attgcttgaa cccaattcta
     8761 atttagaagg aaaaactatt gtattaattg atgatgttgc aaatactggc aaaactatgt
     8821 tttatacttt taatgagctt acaaaataca aaatagagaa gctcattacc tgtgttttaa
     8881 tagacagaac tcataaaaaa tttcctatac agtctgatat tgttggctta caaatggcta
     8941 ctacttttaa tgaacacatt attgtaaaaa ttgaaaacaa cgaaatagaa ggtgcatatt
     9001 tattttaaat actaatattt tttgatgaag aaagaagtaa tatttgcatt tttacttttt
     9061 ataagtacat tttgtttttc tcagaaaaat ggttttaata ttgatgattt agtagagcca
     9121 aatgaacaag aattattgat tattgaacgc ataaattatc ttaatgaaat gcgaaaatta
     9181 agttccaatt tattttggaa agattttggt ctagatgagt ttccaggcac tattgtatat
     9241 tttacagatt cactttccta ttttatcaat ccagaacaaa gtgttttaga taaaatatca
     9301 aaatttaaag cacttaaaaa tccctatgat tatacaattt taagattagc acttccattt
     9361 gatgcaccaa cctttgtaat ccaaacacta tttgaaacat cagaaggaaa taaaaaaaat
     9421 attaactata tgttgccgat tttattttgt agttctccag aagtaagtaa aactttacaa
     9481 ccagaaatta atagcacgga actttgggca agcaatacat tgcacgaata ttttcaccaa
     9541 tatcaattca agataaaagc catttatagt tatcttaatg tctttatttc gcaaaagaaa
     9601 atgtttgaca aagacagtat gcaatccatt tacaacaaaa actggaactt taaagatacc
     9661 ttagtcaaag aaaatgattt attacttaaa gctattaatt ccaatagcct tgaagccgaa
     9721 aaaaattatt ttactcaatt tctaaaatca agaaataaaa ggcgattaga ataccaaaag
     9781 aaaacaaagt ttgcaattgg tcaaattgaa gatttatggg aaaaaattga aggtacaact
     9841 ttatatcttg aagctgtact aaaacaaaac tttgggcaac ttcctcctca acattaccta
     9901 atacaacacg atactttatt tttaaatcaa aatattttta gtgggttcaa tcttgcaact
     9961 gatttaaaat acacatcaat ccaagatgca gattattatt ttggagcaac tggtgtaaat
    10021 ctaattcggc tattagaaaa acacaatgtt gattataaaa aggatttctt ccaatatgca
    10081 tcaatgcctt tatctactca attaaagtac ttttataaaa taaaataatt caatgttctc
    10141 aaaattattt tctaagaaaa caattattct attatgcacg tttgtattcc taataacaat
    10201 ttcagctatt gttttttgta ataataaagt cgagcagttt tcaaatgaca aattatacaa
    10261 cgatattaat aaaatccctc acaatcatgt aggcttactt ttaggaacat gcaaaacatt
    10321 aaaagacaga attactatca atcctttttg gcaacatcga ttagaggcaa cttatttact
    10381 ttggaaacat aaaaaaatag atataatatt gatcagtggt gatggcggat ggcatggcta
    10441 taacgaacca gaagatttta gaaaagcatt tttgaaatta ggcgttcctg attccgtgat
    10501 agttatagat gctgctggat ttagaacgca cgattctgtg attcgagcat tgaaaatatt
    10561 tggtttaaaa aagtttacta ttatctctca actatttcat aataaaagag cattagttat
    10621 tgcaaataaa tacaatattg atgcaattgg gtttaatgca aatgatatag gcagaaaaca
    10681 tgcttggttt aattatttta gagaaaaatt agcaagagta aaattattct tagatttata
    10741 tattctcaac acacagccac accttttaga tgatacaaga tttattaatt aagtaaataa
    10801 caaatgaaaa tgaaatatat tttagcatta gaccaaggca ctactagctc aagagctatt
    10861 ttgtttgatg aatcaagtaa tattgttgcc gtagcacaaa aagaatttac acaaatattc
    10921 ccacaaccag gttgggtaga acataatcct gaagaaattt ggcaaacaca gttagaaacc
    10981 gctaaagaag tcatcgtaaa agccaacata ttaccatcaa atattgcagc tattggcatt
    11041 accaaccaaa gagaaacgac cgtaatttgg gataaagaaa ctggaaaacc aatttacaat
    11101 gcaatcgttt ggcaagacag aagaactgct agtttttgtg atgagctaaa agctagagat
    11161 ttacaaaaat atgtaggcga aaacactgga ttagtcatag atgcatattt ttcaggtaca
    11221 aaaatcaatt ggattttaaa tcatgtggaa ggagcaagag aaaaagctca tcaaggaaaa
    11281 ttattatttg gaactataga tacttggcta atttggaact taactaaagg tactgttcac
    11341 gcaacagatt atacgaatgc ttccagaacc atgatatata atattaaaaa tttagaatgg
    11401 gataaaaaac tctgtttaga tttagatgtt ccaacttcta ttttaccaca agtattaaac
    11461 tcttcagatg attttggaaa gacacatacc gatattctat ttaatgaatc tattccaata
    11521 acaggtgttg ctggcgacca acaaagtgct ttatttgggc aagcctgttt tgaaaaagga
    11581 atggcaaaaa atacttatgg tactggttgt tttatgctaa tgaatacagg agaaaaattt
    11641 gtacaatcaa aatctggatt aatcactaca attgcttggg gattagatgg aaaaattacg
    11701 tatgcattag aaggtagtgt ttttgtggct ggtgctgcaa tacaatggct tcgtgatggc
    11761 ttaaaattaa tagatactgc aaaagattca gaatattttg ccttaaaagt agatgatact
    11821 ggtggtgtgt atgtagtgcc tgcttttgtt ggtttgggtg caccctattg gaatatgtat
    11881 gcacgtggag caatttttgg actaactaga ggaactggaa aagcacattt aattagagca
    11941 acattagaat ctctagctta tcaaaccaat gatattttac aagcaatgca acaagattca
    12001 ggtataaatc taagtgaatt aagagtagat ggtggagctt gtgccaataa tttattaatg
    12061 caatttcaag cagatatcat agatgtacca gtagaaagac caaaactcat agaatccacg
    12121 gcattaggtg ctgcattttt cgctggtatc tatgttggac tttattctat tgaaaaaatc
    12181 aataaaataa aagccataga ccataggttt gaaccaaaga tgcaagaaga gaaaagaact
    12241 aagcttctta aaggttggaa aaaagcaatt aaacgagcga tagattggga gttagacgaa
    12301 taattaaaaa caaatattaa tataatattt atttaacaaa gaaaatattg ctaaataaaa
    12361 ttctttgtaa tatttttgtc ctcgaattta aactaattaa aaaaaatcaa aatgaaaaaa
    12421 ttattcgtat tatttgctgc tgctacatta ttcgtaggag tacaagcttg taaatctact
    12481 gaacaaccag ctgaagaagt tgttgaagct actgaagaag ttgttgaaga cgctgctgct
    12541 gtagttgatt ctgctgctgc tgtagttgat tctgctgcta ctgtagttac tgaaacagtt
    12601 gctaagtaat ttacttaaca agtaaaattt aagccccaat ttttttgggg ctttttttat
    12661 ttttttaaca ttccattctt ttaaatttta gctccaatta ttatatttgc aacaactaaa
    12721 tcaatcaaaa atgaagaaat tatttgtatt atttgctgct gctacattat tcgtaggagt
    12781 acaagcgtgt aaatctactg agcaaccagc tgaagaagta gctgctactg aagaagtagt
    12841 tgaagaagct ccagctgtag ttgattctgc tgctgctgta gttgattctg ctgctactgt
    12901 agttactgaa acagttgcta agtaatttac ttaacaagta aaaaaatcaa taggctgttc
    12961 gaaaagtttc gggcagccta tttaatttat actaatattt ttgaatgttt tttttctgaa
    13021 atagacacac gcattaatat aaaaattgtg taaaaatgaa agctagtaaa ttgatttcaa
    13081 aattaaaata taaaactgta atttttttta aaatatcaca attttcactt ttaattctat
    13141 atttatcgtc aatgaggatt tttctttttg tttttctaat ttgtaatgta ttatttgtaa
    13201 aagctcagta ttttattgat aatgcagtag ctccgcaatc tcatttggct ttatggtata
    13261 agcaacctgc tgagaattgg atgacttcgg ctctgccaat tggtaatgga agaattggag
    13321 caatggtttt tggtggcgta attcaggaag aaattcagtt taatgataaa actttatgga
    13381 ctggcaataa aaatacaaga ggctcctatc aaaattttgg caatatcctc ttaaattttc
    13441 ctaaattgga tactattgag cactacagaa gaatactcga tttggaaact gctacttgtc
    13501 aaatagacta taaggctagg aatatcaatt ataaaagaac ttattttgct agttatcctg
    13561 ataatgcaat aataatgcaa ttctcaagcg atacggaagc cgcaatctca tttgatattt
    13621 tattaaacga tgctcattcc tcagagataa aaattttaga caattcaatg gtttttagag
    13681 gaaagttaga tatactttct tatgaagcac agttaagcat aaaacatgaa ggtggtattc
    13741 ttaaaaagga taatcatcaa ttaagtattc aaaatgccaa tactgttact ctaattctaa
    13801 ctttaggtac taattacaat ccagttgaag ccgattatat tggttatgat gcaactgaat
    13861 tgagttcaat aatttctaat caaatgtcat cagcttattc taaaacttac gatactttat
    13921 ttgctcgaca tgtttcagat tataaatcat tatttgatag agtgcatttg aatttgggaa
    13981 atacaaatcc aagtattcct accgatcaac ttcaattatt gtataataaa ggagacagaa
    14041 atacggcttt agatgtttta tattttcaat atgggagata tttaatgatt tcaagtgcta
    14101 gaggaattgc aatgccctct aatttacaag gattgtggaa tgatagcaac ttaccagctt
    14161 ggggttctga tattcatacc aatataaatg tccaaatgaa ttattggcct gctgaaataa
    14221 ccaacctttc agagttacat agttcattga cagattatgt ttttaatgaa gcagttttgc
    14281 aacctgcatg gcaagaaaat gccaccactc taggagcaaa aggctggact ttaagaacac
    14341 aatgtaatat atttggctat tcagattgga aatggaatag acctgctaat gcttggttag
    14401 cgatgcatct atggcaacat tatacatata cattagatac agtctatttg caacaaaaag
    14461 cttatcctgt gatgaaatct gcatgtgatt tctggttgtc ttatttaata aaagataaaa
    14521 atggaaagct agttgctcca aaggaatggt cgccagaaca tggtccttgg caagatggtg
    14581 tggcttatgc acaacagctc atttttaatt tatttgaaaa taccttaaaa gcaaatgaga
    14641 ttttaggtga gaataactct tatcatatta ccttgaaaaa tacattatta aatttagata
    14701 atggtatagc aatcggacat tggggacaga ttaaagaatg gaaaaactat aaacgtggtg
    14761 agaataaaaa gaaaaaacaa catagacata tatcacattt gattgcatta tatcctggca
    14821 ataaaatttc tcccttactt gataaaaaat actcagatgc tgctataaaa tctttaaacc
    14881 aaagaggaga ttctggtcct gggtggagta gagcatggaa aattaatgct tgggcaagat
    14941 tactagatgg agagcgagct tatcaactta tccaacaagc attgcaaaat acaacttctg
    15001 caagtactaa ttttgatgaa aatggtggag catatcaaaa tttattagat gctcaccctc
    15061 ctttccaaat agatggtaat tttggagcca cttcgggaat tgcagaaatg ttattacaaa
    15121 gccatttgga gaagattcaa ttactgcctg ccgtgccaaa agcttggtcg aaaggaagtg
    15181 tgtatggatt aaaagcaatg aataatttta cggtagatat aatttgggat ttagatcttc
    15241 aaactcaagc gattatccac tctggttctg gaaagatatg ttctatttat ttcaaaaata
    15301 tagcgaatgc gaaaattact gacaataatg gtcgtcctgt accttttatc gtaattaata
    15361 atgacgaaat taaatttcct acatcaaaag gtttgagtta tacaattag
//
LOCUS       DAOQCW010000058        15244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1414_length_15244_cov_5.550069, whole genome shotgun sequence.
ACCESSION   DAOQCW010000058 DAOQCW010000000
VERSION     DAOQCW010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15244
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1414_length_15244_cov_5.550069"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="PLS16_06570"
     CDS             complement(<1..568)
                     /locus_tag="PLS16_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HNJ60779.1"
                     /translation="MNTVELVESFSEFKEFKNIDRPTMMRVLEDVFKTLLRKKYTSDQ
                     NFDIIVNTDKGDLEIWRHRNVVEDGQVQDKLAEVELSYAKTIEPDMEVDEELYEPIPL
                     EIFGRRAILAARQTLISRILDLEKDEVYKKYKDRIGDIISAEVYQVWRKEILLLDDEG
                     NELILPKNEQIKTDFYKKGDTIRAVIKDV"
     gene            complement(574..1050)
                     /locus_tag="PLS16_06575"
     CDS             complement(574..1050)
                     /locus_tag="PLS16_06575"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60780.1"
                     /translation="MENSIQLKEQIEQYLNNNIDNTTMFVVDVKVLPTNKIEVFLDRS
                     DTNITIDECAKISRKLEEYLESNQLVGEKYTLEVSSPGMDQPFKVPQQFLKNIDKNVE
                     ILMNDGTKDIGKLIAYNTDSVEIQTEKKVKKEIITENKTILLSEIKSIKKHFIFKI"
     gene            1237..1701
                     /locus_tag="PLS16_06580"
     CDS             1237..1701
                     /locus_tag="PLS16_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60781.1"
                     /translation="MAIPQFEHLNNEEKELLFDVPAIITLLIGSADDDLNSEEISTGL
                     RIILVRKDSGDKLLQDYYEATNNVFDSSLNKYSLAYENLATEERIEKLSVILAQLDTI
                     LPTIDPLYATTLIKSFRTFAKEIAEASGGFAGIASVSNAEHQLVSLPMISFH"
     gene            1764..2207
                     /locus_tag="PLS16_06585"
     CDS             1764..2207
                     /locus_tag="PLS16_06585"
                     /inference="COORDINATES: protein motif:HMM:NF024822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HNJ60782.1"
                     /translation="MSKLVKLMDSAFKIPGTNRTFGIDPILNLIPYGGAVAGYLISAY
                     LLISMFKNGASSKVIGKMIGNITLDAVVGAIPFLGTVFDFMFKANKRNLTLAIEHFEE
                     GKHQGSILPYLIPIIIILIALFAFVVYITIIAFRFFFDILNQIQL"
     gene            2274..3260
                     /gene="ppk2"
                     /locus_tag="PLS16_06590"
     CDS             2274..3260
                     /gene="ppk2"
                     /locus_tag="PLS16_06590"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HNJ60783.1"
                     /translation="MAKVNPNTKAKNLEVQKTNVPNTKETVLPLENKNGVDSTNDTKV
                     EEQSILIRDLNNLKSKKDLAELIKSEGASPNKILSNIAYEEQLHDLQIELVQLQRWTQ
                     ETGARIAVLFEGRDAAGKGGTIRRFTEHINPRAMRVVALPKPSDEEKGQWYFQRYIKQ
                     LPNKGEIVFFDRSWYNRAVVEPVNNFCTQQQYEVFMQQVTEFEHMLYEDGVIIIKFWF
                     SISKEEQLRRFESRKKNPLKQWKLSPVDMQAQEKWDIYTKYKEEMFRRTHTSFSPWII
                     VKANNKQKARLESIRYVISLIPYVDKISTKTRTHPDPNIVARYHRKSQQVDN"
     gene            3350..3706
                     /locus_tag="PLS16_06595"
     CDS             3350..3706
                     /locus_tag="PLS16_06595"
                     /inference="COORDINATES: protein motif:HMM:NF037003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60784.1"
                     /translation="MNENKNQHELNVCLDECLLYIHSFLKGKKTVVKTLQIGLADGKL
                     ANTIVNATQKPHIVVDPRQEMRQFSGIKFLKAENIGISRDKVVRDLWLHQDLGKIGAK
                     KSFTIPAHGIVVLKTN"
     gene            complement(3708..5477)
                     /locus_tag="PLS16_06600"
     CDS             complement(3708..5477)
                     /locus_tag="PLS16_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNJ60785.1"
                     /translation="MNIDYKKIGLSLMVIVVIIAFYAISFIKKDKQSVAVNNFDLTKI
                     KERGILRVITEYNSISYFIYKDEVAGFDYEIMERFAESIGVRTEYIIAHNHQEMYNFL
                     RQGKGDIIATGLYQTNESGVAYSIPYRKSEQVLVQRKAGKYTRKSDSTTSYYQPIIDI
                     KSLEAKDVFLTLNSPYIEQVKHLADTLGFNVNINLVSDERSIEDLINAVADAEIDYTV
                     ADKDLALTNNSFLGNLDLGIQFGKEKDLHYATRKSSKELQIILNEWLSHFIKSKDYKQ
                     IANKYFKFDRKIIDQFNETEYLTKGQISLYDNIIQYFAKSINWDWRLVAALMYQESKF
                     DPNATSWAGAKGLMQLMPGTARQMGLHTGLYQPEQNIQAGTKYLQYLEQFWKEVPDFT
                     QRIKFILASYNAGVGHIQDAARLAKKYGYSETEWDGNVEYFILYKSNPRFYNDRVVKY
                     GYARGSETFNYVRNIIKKYFYYTDNINDSTNLDFALQQKDLIPFNGIDGVYNPSVGLL
                     QERVRKELFVSKKLFEQNQDLVPLNKKNNPFDKAPNELFKEKGKSKKLFEQKSTLFRK
                     NDTLSNQQLMIKNESKINQLEPR"
     gene            complement(5524..6315)
                     /locus_tag="PLS16_06605"
     CDS             complement(5524..6315)
                     /locus_tag="PLS16_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HNJ60786.1"
                     /translation="MIKDIIKNKSTRLFIILGGFFIANALIAETIGVKIFSLEATFDL
                     PPANIPIFGYPFSFNLTAGVLLWPVVFVMTDIINEYYGKRGVKFLSNLTVGLLAYAFL
                     IYYLAIQLVPADWWITSKTLNGVNDMQAGYQQILGQGAAIIFGSLTAFLLGQVIDVGI
                     FHKIKQMTGEKYIWLRSTGSTVVSQFIDSFVVLFIAFYFVPKYLTGQPWPFKMILAIG
                     VGNYIYKFIVAIVLTPVIYFAHHYIDKYLGDEIATKMKNAATQNN"
     gene            complement(6330..7676)
                     /gene="tilS"
                     /locus_tag="PLS16_06610"
     CDS             complement(6330..7676)
                     /gene="tilS"
                     /locus_tag="PLS16_06610"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNJ60787.1"
                     /translation="MLEQVEKYIQDNNLANKGTPILVACSVGVDSMVLVKLLQLLTFP
                     ISIAHCNFQLRDEESDGDESFIRTYAEENNIPLFVKRFETIAYKKNSKISTQMAAREL
                     RYEWFEQIRKENNFHSIATAHHLDDQLETILMNFAKGTGVNGLSGMQAKNNFIIRPLL
                     QIPKQSILQFAQDNHLAFREDSSNLTDDYQRNKIRHHITPKLLEINPNLYENVLQFTS
                     IMQDTQTLLALQIDAIRKKCVSIKNGNTIIKINYVKQHPAKKTVLFLLLKDFGFTDIH
                     IEQIFKIKESSKQFFSETHRLIIDRNELIITSIRHEILSHLLMDKIPNNIQFNQYKIQ
                     CSVVPIEHLNIKDSKNYAYIALEKLEFPLMLRYLKEGDYFYPFGIGKSKNPLKVGKKK
                     IGKYFRDEKLSLLEKEQTPILFSKERAVWLVGHRIDDRFKVTGTTKEVLKFKIIKG"
     gene            complement(7677..8654)
                     /gene="lpxD"
                     /locus_tag="PLS16_06615"
     CDS             complement(7677..8654)
                     /gene="lpxD"
                     /locus_tag="PLS16_06615"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNJ60788.1"
                     /translation="MFTLDKIAEYIGASIEGNKDYQVEAIAKIENATAQHISFLTNEK
                     YIDYVNKNEIGILLVSDAIYQTIKSKNIATNQIKGGFLILNDAYAALPKVLELFATQS
                     NENQEYKIGTNSTIYPNVFIGTNVQIGNDCIIYPNVSIYADCIIGNRVIIHSGAVIGA
                     DGFGFAPQKDGTYNKIPQLGNVIIEDDCEIGANTCIDRATIGSTIIRKGVKLDNLIQV
                     GHNVEIGEHTVIAAQTGIAGSTKIGHHNMIGGQVGFVGHISIAPYTKINAQSGVAQSV
                     KTENEIIGGAPAFSLKQFFKSSIVFKQLPELQKQITTLQNEIQKLKEEK"
     gene            8729..9157
                     /locus_tag="PLS16_06620"
     CDS             8729..9157
                     /locus_tag="PLS16_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HNJ60789.1"
                     /translation="MRIWKVGDIPIDALEKRKKGTLMEALGMEYVEIGDDFIEARMPV
                     DERTRQPFGILHGGAHVALAESIGSLGANLCLDNTKEYAVGLDINSNHLKSVSSGFVH
                     GIAKPIHIGKTTQVWEIKIYDENKKNLLNITRLTMMIKQI"
     gene            complement(9220..9909)
                     /locus_tag="PLS16_06625"
     CDS             complement(9220..9909)
                     /locus_tag="PLS16_06625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNJ60790.1"
                     /translation="MLKRFYSNTLPLILDADSRHAAKTLRCKEGDKIEVIDGQGKLFE
                     AIIFHIGRNEIQLNDIKLIQEATNVQLLSIAIAPTKNPSRIEWFIEKATELGVKNIYP
                     IQTKRTEKSSIKEERLNNIIISAIKQSKQLYLPTLFPLQPFKEVIRQTQSIPQKFIAH
                     CADNPVRLQNVFQNDKEAIVFIGPEGDFTTDEILLAQQNGFVPISLGNSILRTETAGV
                     YVTGLIRMLLG"
     gene            complement(9915..10511)
                     /locus_tag="PLS16_06630"
     CDS             complement(9915..10511)
                     /locus_tag="PLS16_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60791.1"
                     /translation="MKVLFYILFIFLLSGCAATYKSFTVEQFPYKELQTENPKIDYAT
                     RQGVMFNTNNFKYAKREQKQAYNLIAFKIVNKSTEPINVSDFKYSCGGATVITPISMD
                     TYIKENKQKAGLYWLYLGLVSPNPFMGKNGLGKVIPVGAAPAIINFALAHKANKKMKQ
                     NIGAYDLTNKVIQPNDTIYGILPFKGSSNCGDIFITLP"
     gene            complement(10557..11036)
                     /locus_tag="PLS16_06635"
     CDS             complement(10557..11036)
                     /locus_tag="PLS16_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60792.1"
                     /translation="MPYHKLPDDAKVWIYQADRPIEIDELNFINDLIEAFVEQWQLKT
                     QDILGYAAVYYRRFIVIMADVGSIGKEAIEEPIEMIHYIENELGIDFFQDKAICYKIG
                     LQDIDSFYLEDIERLIDNGTLSKETLIFDNHIHIKVDFENKWGKLRAIDTDFSKYFS"
     gene            complement(11076..11837)
                     /locus_tag="PLS16_06640"
     CDS             complement(11076..11837)
                     /locus_tag="PLS16_06640"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNJ60793.1"
                     /translation="MENNQNLLKALIIAIAIILSAFIFKSAVKNRNANEDTISVTGLG
                     TVDFVSDEINWSGSFNVVAPDAKTAYNTIVADKKKVRDFFISKGFKDNEFVFGGAEVN
                     KKFRDISYETSNGGVRYESVFDGYLATQTISFYSKKNDALMSKIEKVKQETAELINQG
                     IEFTSNETQYTYSDLPSLKHNLIESATKDARERAEKIVKNGKGSLDKLKSASMGVFQI
                     TGRDSNEDYSYGGINDTYSKEKSARITVRLTYILD"
     gene            complement(11903..13135)
                     /gene="lysA"
                     /locus_tag="PLS16_06645"
     CDS             complement(11903..13135)
                     /gene="lysA"
                     /locus_tag="PLS16_06645"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNJ60794.1"
                     /translation="MEIVNNEYQISGVSVLEVCKEFGTPLYVYDANVIERNYNRLINA
                     FKTTNVEIHYACKALTNINILKLMKKIGANLDTVSIQEVQIGLKAGFAPQEIMYTPNC
                     VGMEEISKAVSLGVKINIDNISILEQFGNKYGDTYPICIRINPHIMAGGNTKISTGHI
                     DSKFGISVYQLRHIERVVKSTNLKVEGLHMHTGSDILDVDVFLNGAELLFDAAKHFKD
                     LEFLDLGSGFKVKYSKDDYSTNIEELGEKISERFNAFCTAYGKELKLIFEPGKFLVSE
                     SGFLFVNVNVIKQTMSTVFAGVDSGQNHLIRPMFYDAYHHITNVSNPEGKNRIYTVVG
                     YICETDTFGWDRKLNEVREGDILCLHNAGAYGFSMSSNYNSRYRPAEVLIKDGKAILI
                     RRRENLEDILASQIDAEI"
     gene            complement(13212..13583)
                     /locus_tag="PLS16_06650"
     CDS             complement(13212..13583)
                     /locus_tag="PLS16_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60795.1"
                     /translation="MRVVVHIFILIAVVTFFVIYNFSGANNHNSKSEIVIVGNDTLGR
                     NWDNLPSAKEIEAARFKENANNATMNFNFGRQNEVTQKEDPNTFESIGFSNRIGESQN
                     NRETIISDNGAEIVVGTYENY"
     gene            complement(13674..>15244)
                     /locus_tag="PLS16_06655"
     CDS             complement(13674..>15244)
                     /locus_tag="PLS16_06655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HNJ60796.1"
                     /translation="QKNNNNKYMSLDVMVILIGSIFCGLMILFTYLTNRDIQPKQLFL
                     LFFTEMWERFSFYGMRALLILYMTNVLLYKDKDSNLMYGAYNALVYITPLFGGILADR
                     ILGFKKSIIFGGVMMAIGHLVLAIPMNQTFFLGLGFLIVGNGFFKPNISSFLGKFYEE
                     NDKRRDAGYAIFYMGINVGGFLGSAICGYLGQKISWHLGFGIAGVFMIIGLITFLLNR
                     DKLEGKGGVSDATFLSKKSLIGIDWEKTIYLGSVLFVFVAILMIVFNDITQIFFAIIG
                     LITIVYILFVASKEEIKPKQMLWAALVMIIFSSLFWAFYEQAGGALNLMAERNVDMKV
                     GSTELSSAMVNNSINPFYIIFLTPLFAFMWNWLDKRNLKPNDPLKFGFAFVFLAIGYY
                     AFVYGGNKGIDIGYMPLLYFLLGYFFITVGELFLSPIGLSMITKLSPPKIIGFMMGTW
                     FLASAFGHELAGWIGSKMAIPEANPDGSVFTAIQSLPIYLAGCNQIALVSVIAAVFIM
                     ISSLIVKKWMHGVN"
BASE COUNT         5250 a         2508 c         2065 g         5421 t
ORIGIN      
        1 ctacatcttt aataacggca cgaatcgtat cgcctttttt atagaaatca gtttttattt
       61 gttcgttttt aggtagaatt agttcgttgc cttcatcatc taaaagtaaa atttcttttc
      121 tccatacttg gtatacttct gctgaaataa tatctccaat tctgtcttta tattttttgt
      181 atacttcgtc tttttctaag tctaaaattc tagatattag tgtttgtctt gctgcaagaa
      241 tagctcttct tccaaatatt tctaatggaa ttggctcata caactcttca tctacttcca
      301 tgtctggctc tattgtttta gcgtaagata gttctacttc tgctaattta tcttgtactt
      361 gtccatcttc aacaacattt ctatgtctcc aaatttctaa atcacctttg tctgtattta
      421 ctattatgtc aaagttttga tctgaagtgt atttttttct aaggagtgtt ttaaagacat
      481 cttccaagac acgcatcatt gttggtctat ctatgttctt aaactcttta aattccgaga
      541 acgactctac taattctaca gtattcatgt tttttaaatt ttaaaaatga aatgtttttt
      601 tattgattta atttctgata ataatattgt tttattttct gtgattattt ctttttttac
      661 ttttttttct gtttgaattt caacactatc tgtattgtat gcaattagct ttccaatatc
      721 ttttgtgcca tcattcatta atatttctac gtttttatct atatttttta gaaattgttg
      781 tggcacttta aatggttggt ccatgcctgg tgatgatact tctaaagtat atttttcacc
      841 tactagttga tttgattcta aatattcttc aagttttctg ctgatttttg cacattcatc
      901 tatcgtaata tttgtatctg atctatcaag aaatacttct atcttatttg ttggcaatac
      961 tttaacatca actacaaaca tagttgtgtt gtcaatattg ttgtttaaat attgctcaat
     1021 ttgttctttt aattgaatag aattttccat tattttttat aaataaaaag aggactttgt
     1081 caagtcctct catcaatctt ggtgcaaaga taagtataaa tttgtataaa acaaatattt
     1141 tttgcttttt ataagcaact aaacttaaat tatcttaata tggctaaaaa attagctgag
     1201 aaaatattat tttcgtgcat taattaaata gactttatgg caataccaca atttgaacac
     1261 ttaaataacg aagaaaaaga actacttttt gatgttcctg caattattac attacttatt
     1321 ggttctgcag atgatgattt aaacagtgaa gaaattagta ctggacttag aataatttta
     1381 gtaagaaaag atagtggtga caagttgtta caagattatt atgaagctac aaacaatgtt
     1441 tttgatagct cattaaataa atatagttta gcatacgaaa atttagctac agaagaacgt
     1501 attgagaaat taagcgttat tctagctcaa ctagatacaa ttctaccaac aatagatcca
     1561 ttgtatgcta caacattgat aaaatcattt agaacttttg caaaagaaat agctgaagca
     1621 tctggtggtt ttgcaggaat agcaagtgtg tctaatgcgg aacaccaact tgtatcttta
     1681 ccaatgatta gttttcatta attgtaatta atacattgca aacaataaac gaaaataata
     1741 agcttcccaa ggaattagta aatgtgtcta agttagtaaa gctaatggac agtgcattta
     1801 aaattccagg tacaaatagg acttttggaa tagatccaat tttaaatcta attccatatg
     1861 gtggtgctgt ggctggatat ctaatttctg cgtatttact tataagcatg tttaaaaatg
     1921 gtgcaagttc taaagtgatt gggaaaatga ttggcaatat tactttagat gccgttgttg
     1981 gtgctattcc ttttttaggt actgtattcg attttatgtt taaggcaaat aaaagaaacc
     2041 taacattagc tatagaacat tttgaagaag gaaaacacca aggcagtatt ttaccatatc
     2101 ttattccaat aataattatt cttatagcat tgtttgcttt tgtagtctat attactatca
     2161 ttgcatttag attctttttt gatatactaa atcagattca actctgataa atatcataat
     2221 atcaattaac ttatatcata tatcaagcta ttttattggt ctatttttgc aaaatggcaa
     2281 aagtaaatcc aaacacaaag gcaaaaaatc ttgaagtaca gaagactaat gtaccaaaca
     2341 caaaagaaac agttctacca ttagagaaca aaaatggagt tgattctaca aatgatacaa
     2401 aagttgaaga gcaatctatt ttaattagag acttgaataa tctgaaatca aaaaaagatt
     2461 tagcagaatt aataaaaagt gaaggcgcaa gccctaataa aattctatca aacattgctt
     2521 atgaagaaca gttgcatgat ttgcaaatag aattggtgca attacaaaga tggacacaag
     2581 aaacaggtgc aagaattgct gttttatttg aaggaagaga tgccgcagga aaaggtggaa
     2641 ctatcagaag atttacagaa catataaatc cacgtgctat gcgcgtagta gcattaccaa
     2701 aacctagtga tgaagaaaaa ggacaatggt attttcaaag atatattaaa caactaccaa
     2761 ataaaggaga aattgtcttt tttgatagaa gttggtataa tagagcagtt gtagaaccag
     2821 taaataattt ttgtacacaa caacagtatg aagtatttat gcagcaagta acagaatttg
     2881 aacatatgct ctacgaagat ggcgttatca ttataaaatt ttggttttct atatcaaaag
     2941 aagagcaatt aagaagattt gaatcaagga aaaagaaccc attgaagcaa tggaaattaa
     3001 gtcctgttga tatgcaagca caggaaaaat gggatatcta cacaaaatat aaagaagaaa
     3061 tgtttagacg tacgcatact tctttctcac cttggataat tgtaaaagca aataacaagc
     3121 aaaaagcaag gttagaaagt atcagatatg ttatctcttt aattccatat gtagataaaa
     3181 taagtaccaa aacgcgcaca catccagacc caaatattgt agcaagatat catagaaaat
     3241 cgcaacaagt agataattaa tatttttctt cgcacattgt ctgttaattt ttttctcgtt
     3301 tatataataa attatataaa tttacgacat cttttaatag acctaaaaca tgaatgaaaa
     3361 taaaaaccaa cacgaactaa atgtttgttt ggatgaatgt ctattgtata tacattcatt
     3421 cttaaaagga aaaaaaacag ttgtaaagac attgcaaatt ggattagctg atggcaaact
     3481 tgcaaacacc attgtaaatg ctactcagaa accacatatt gttgttgatc caagacaaga
     3541 aatgcgtcag ttttcaggaa ttaaattctt aaaagctgaa aacattggaa ttagccgaga
     3601 taaagtagtt agagatttat ggttacatca agatttaggt aagattggag ctaaaaaatc
     3661 atttactata cctgcacatg gcattgttgt tttaaaaacc aactaagcta acgaggttct
     3721 agctgattta tcttactttc gtttttaatc atcaattgtt ggttacttag tgtatcgttt
     3781 ttacgaaaca aggtactctt ttgttcaaaa agttttttgc ttttaccctt ttctttaaat
     3841 aattcattag gagctttatc aaaaggattg tttttcttat ttaatggaac taaatcttga
     3901 ttttgttcaa agagtttttt agaaacaaat agctcttttc taacacgttc ctgtaataac
     3961 ccaacacttg gattatacac accatctatt ccgttaaatg gaatcaaatc tttttgttgt
     4021 agagcaaaat ctaaattggt tgagtcattg atattatcag tataataaaa atatttttta
     4081 atgatattac gaacataatt aaaggtttcg cttcctcttg catagccata ttttaccacc
     4141 ctatcattat aaaatcttgg attagattta taaagtataa aatattctac attaccatcc
     4201 cattccgttt cggaatagcc atattttttt gccagtctcg cagcatcttg aatatgcccg
     4261 acaccagcat tataggatgc taaaataaac tttattcgtt gtgtaaaatc tggcacttct
     4321 ttccaaaatt gttctaaata ttgtaagtat tttgttcctg cttgtatatt ttgttctggc
     4381 tgatacaaac cagtgtgtaa acccatttgt cttgctgttc ctggcatcaa ttgcattaag
     4441 ccttttgctc cagcccacga agttgcattt gggtcaaatt tagattcttg atacatcaaa
     4501 gcagctacca aacgccaatc ccaattgata cttttggcaa aatattgaat aatattatca
     4561 tatagcgaaa tttgcccttt agttaaatat tctgtttcat taaattggtc aattatcttt
     4621 ctatcaaact taaaatactt attggcaatt tgtttgtaat cttttgattt aataaaatga
     4681 ctcagccatt catttaaaat aatttgcaat tcttttgaag attttcgagt tgcataatgc
     4741 aaatcttttt ctttcccaaa ctgaatccct aaatctaaat ttccaaggaa tgaattattt
     4801 gttagggcta aatctttatc ggcaactgta taatcaattt cagcatcagc aactgcatta
     4861 attaaatctt cgatacttct ttcatcactc accaaattga tatttacatt aaatccaagt
     4921 gtatccgcta ggtgttttac ttgctcgata taaggcgaat ttagtgttaa aaaaacatct
     4981 ttcgcttcca atgattttat atctattatg ggttgataat aagaagttgt actatctgat
     5041 ttgcgagtat attttccagc ttttcgttga actaatactt gttctgattt tctatacggt
     5101 atagaataag cgaccccaga ttcattggtt tgatacaaac cagttgcaat gatatcccct
     5161 tttccttgtc tcaaaaaatt atacatttct tgatgattat gagcaataat atattccgtt
     5221 cgcacaccaa tactttctgc aaaacgctcc attatttcat aatcaaaacc agcgacttca
     5281 tctttataaa taaaatagct aatggaatta tactctgtaa tgacacgcaa gatgccacgt
     5341 tcttttattt ttgtcaagtc aaaattatta actgcaactg attgcttatc tttttttata
     5401 aatgaaatgg catagaaagc gataatcaca actattacca ttaagcttaa gccaatcttt
     5461 ttataatcaa tattcaaaac gatacaaatt tatagaataa ttgacaacaa aaactatgca
     5521 attctaatta ttctgtgtgg cggcattttt cattttggtt gcaatttcat cgcccaaata
     5581 tttatcaata taatgatgtg caaaataaat gactggcgta agcacgatgg caacaataaa
     5641 tttataaata taattcccaa cgccaatggc taaaatcatt ttaaaaggcc agggttgtcc
     5701 tgttaaatat ttaggcacaa aataaaaggc aataaacaat accacaaagc tatcaataaa
     5761 ttgagaaacg actgtggagc ctgtggaacg taaccagata tacttttcgc ctgtcatttg
     5821 tttaatttta tgaaaaatac ccacatcaat tacttgtcct aataagaatg cagttaatga
     5881 accaaaaata attgctgcac cttgtcccaa tatttgttgg taacctgctt gcatatcatt
     5941 tacaccattc aatgttttac tcgtaatcca ccaatcggca ggtactaatt gaatggctaa
     6001 ataatatatt aaaaaagcat aagccaataa accgacagtt aaattagaca aaaattttac
     6061 acctcttttg ccataatatt cattgataat atctgtcatc acaaacacaa caggccaaag
     6121 caatactcca gctgtcagat taaaagaaaa tggataacca aagattggaa tgtttgcagg
     6181 tggcaaatca aaagtagctt ctaaagagaa gatttttaca cctattgttt ctgcaatcag
     6241 tgcatttgca ataaaaaaac cacccaagat gataaataag cgagtacttt tattttttat
     6301 aatatctttt atcatttgta gattttaaac tatcctttaa tgattttaaa ttttaaaact
     6361 tctttagttg ttccagttac tttaaatcta tcatcaattc gatggcctac caaccaaaca
     6421 gcacgttctt ttgaaaataa aattggcgtt tgttcttttt ccaataaact caatttttca
     6481 tctctaaaat attttcctat ttttttctta cccactttaa gcggattttt agattttcca
     6541 ataccaaaag gatagaaata atcgccttct tttaagtaac gaagcatcaa tggaaattct
     6601 aatttttcta aagcaatata ggcataattt ttactatcct taatgtttaa atgttcaatt
     6661 ggcacaacag aacattgtat tttatattga ttaaattgaa tattatttgg aattttatcc
     6721 atcaatagat gtgataatat ttcatgccta attgaggtaa taatcaattc atttctatct
     6781 ataattaaac ggtgcgtttc agaaaaaaat tgcttactac tttcttttat tttaaaaatt
     6841 tgttcaatat gaatatccgt aaatccaaaa tctttcaata gtaaaaacaa taccgttttc
     6901 tttgcaggat gttgttttac atagttgatt tttattatcg tattaccatt ttttatacta
     6961 acacatttct ttctaatagc atcaatttgt aatgccaaca aggtttgagt gtcttgcata
     7021 attgatgtaa attgcaatac attctcatat aaatttggat ttatttctaa taattttggt
     7081 gtaatatgat gtcgtatttt attacgttga taatcgtctg ttagattgga actatcctct
     7141 ctaaatgcta gatgattatc ttgtgcaaat tgcagaatag attgttttgg tatttgtaat
     7201 aaaggtcgaa ttataaaatt atttttggct tgcataccac ttaaaccatt tacaccagtt
     7261 cctttagcaa aattcatcaa tatagtttct aattgatcat ctaaatggtg tgcagtggct
     7321 atgctatgaa aattattttc ttttctaatt tgttcaaacc attcatatct taattcacga
     7381 gcagccatct gagtagatat tttagaattc tttttatacg ctatggtttc aaatctcttt
     7441 acaaacaacg gaatattatt ttcctccgca taagttctaa taaaactttc atcgccatca
     7501 ctttcttcat ctctcaattg aaagttgcaa tgagcaattg aaattgggaa agttaataat
     7561 tgtagtaact ttaccaaaac catagaatct acaccaacac tgcaagctac taaaatcggt
     7621 gtgcctttat tcgctaaatt attatcttga atatattttt caacttgctc tagcatctac
     7681 ttttcttctt ttaatttttg aatttcattt tgcaaagtag taatctgttt ttgtaattct
     7741 ggtaattgct taaatacaat tgatgattta aaaaattgtt ttaaagaaaa ggcaggtgca
     7801 ccaccaatta tttcattttc agtttttaca ctttgagcta caccactttg agcattgatt
     7861 tttgtataag gtgcaatact aatatgtcca acaaaaccaa cctgacctcc aatcatatta
     7921 tgatgtccga ttttggttga acctgcgatt ccagtttgag cagcaattac agtatgttcg
     7981 ccaatttcca cattatgtcc tacttgaatt aaattatcca acttcacacc tttacgaatg
     8041 attgtagagc ctattgttgc tctatcaata caagtatttg caccaatctc acaatcatct
     8101 tcaataataa cattccctaa ttgtggtatt ttattataag ttccatcttt ttgtggtgca
     8161 aaaccaaaac catcagcacc aattactgca ccagaatgta taattacacg attcccaata
     8221 atacaatctg cataaattga aacatttgga taaataatac aatcattgcc aatttgaaca
     8281 tttgttccaa taaaaacatt aggataaatc gtagaatttg tacctatttt atactcttga
     8341 ttttcatttg attgagttgc aaataattct aatacttttg gcaaggcagc ataggcatca
     8401 tttagaatca gaaatccacc tttaatttga ttggtagcaa tattcttaga ttttattgtt
     8461 tgatagattg catcagaaac taataaaata ccaatctcat ttttatttac ataatcgata
     8521 tatttttcat tagtaagaaa actaatatgt tgagctgtag cattttcaat ttttgcaata
     8581 gcctcaactt gataatcttt attaccttcg atgctggcac caatatattc tgctatttta
     8641 tctaaagtaa acaaatacga ctatttctga cgaaagtaaa aaaaagaacg caataattta
     8701 ttaagtttga taaacaaaac aatacaaaat gagaatttgg aaagtaggtg atattccaat
     8761 agatgctttg gaaaaaagaa aaaaaggtac tttaatggaa gctttaggta tggaatatgt
     8821 agaaattggt gatgatttta ttgaggcaag aatgcctgta gatgaaagaa ctcgacaacc
     8881 atttggaatt ttacacggcg gtgcacatgt cgctttagct gaatctattg gaagtttggg
     8941 agcaaattta tgtttagaca ataccaaaga atatgcagtt ggcttagata tcaattctaa
     9001 tcatttaaaa agtgtaagtt caggttttgt acatggcatt gcaaagccta ttcatattgg
     9061 caaaacaaca caggtttggg aaataaaaat ttatgatgaa aacaaaaaaa atctattaaa
     9121 tattaccaga ctaacaatga tgataaagca gatataatta aaataattta gtaatgaaat
     9181 aatttttcaa acttacacac ttttggggta gtgtcttttt tatcctagaa gcatacgaat
     9241 caaacctgtt acataaacgc cagcagtttc tgttctgagt attgaattac ccaaactaat
     9301 gggtacaaaa ccattttgtt gagctaacaa gatttcatcg gtagtaaaat cgccttctgg
     9361 tccaataaaa acgatggctt ctttatcgtt ttgaaaaaca ttttgcaatc gtactggatt
     9421 gtcggcacaa tgtgctataa atttttgtgg tatggattgt gtttgacgga taacttcctt
     9481 aaaaggttgt aaaggaaata aagttggtaa gtataattgc ttactttgtt taattgctga
     9541 gatgataata ttatttaatc tttcttcctt gatacttgat ttttcagtcc ttttggtttg
     9601 aattggataa atatttttta cacctaattc tgtagccttt tcaataaacc attctattct
     9661 tgaaggattt ttagttggtg ctattgcaat tgaaagcaat tgtacattcg ttgcttcttg
     9721 aattaattta atatcattca gttgaatttc atttctgcca atatgaaaaa taattgcttc
     9781 aaataatttt ccttgtccat caattacttc aattttatcg ccttctttac aacgaagagt
     9841 ctttgctgca tgtcttgaat cagcatcaag aattaaagga agtgtgtttg aataaaagcg
     9901 tttaagcatt tactttaggg caaagtaatg aatatatctc cacaatttga tgagcctttg
     9961 aatggaagaa taccataaat tgtatcattt ggttgtatga ctttgttggt taaatcataa
    10021 gctccgatat tttgcttcat ttttttattg gctttgtgtg ctaatgcgaa attgataatt
    10081 gctggtgcag caccaacagg aattaccttt cctaaaccat tttttcccat aaacggattg
    10141 ggagatacca aacctaaata caaccaatat aaaccagctt tttgcttatt ttctttaata
    10201 taagtatcca tagaaattgg tgtaatcaca gttgctccac cacaagaata tttaaaatcg
    10261 ctaacattga taggttcagt acttttattt acaattttaa atgcaattag gttatatgct
    10321 tgtttttgct ctcgtttggc atacttaaaa ttgttcgtat taaacattac accttgcctt
    10381 gtagcataat caattttagg attttctgtt tgtaattctt tgtacggaaa ttgttctaca
    10441 gtaaacgatt tgtatgttgc cgcacatccc gacaatagga aaataaataa tatgtagaat
    10501 aagactttca taaaaaataa attttcaaga attgaagata atgaatttgt tgtattttaa
    10561 ctaaaatatt tagaaaaatc agtatctatg gctcttaatt tcccccattt attctcaaaa
    10621 tcaactttta tatgaatatg attatcaaaa ataagggttt cttttgaaag cgtcccatta
    10681 tcgataagtc tttcaatatc ttctaaataa aaagaatcta tatcttgcaa acctatttta
    10741 taacagatgg ctttatcttg aaagaaatct attcccaact cattttcaat ataatgtatc
    10801 atctctatag gttcttcaat tgcttctttg ccaatagaac caacatctgc cataataaca
    10861 ataaatctgc gataataaac agcggcatag cctaaaatat cttgagtttt taattgccat
    10921 tgttcaacaa atgcctcaat taaatcatta ataaaattta attcatctat ttcaattggt
    10981 ctgtctgctt gataaatcca cactttcgca tcgtcaggta acttatggta aggcattaac
    11041 attagactac aaatataaac agaaagaatt taaaattagt ctaagatata tgtcaaacgt
    11101 actgtaattc gagcagactt ttctttgcta taagtatcat taataccgcc ataactataa
    11161 tcttcatttg aatctctacc cgtaatttgg aaaacgccca tacttgcact ttttaattta
    11221 tctaaacttc ctttgccatt tttaactatc ttttcggctc tttctctagc atcttttgta
    11281 gcactttcaa ttaaattgtg tttcaaactt ggtaaatctg aataggtata ttgggtttca
    11341 ttagaagtaa attcaattcc ttgattgata agctccgctg tttcttgttt aactttttca
    11401 atttttgaca ttaaagcatc atttttctta ctataaaatg aaattgtttg tgtggctaaa
    11461 taaccatcaa aaaccgattc ataacgcaca ccgccattgc ttgtctcata cgaaatatct
    11521 ctaaattttt tattgacttc ggcacctcca aaaacaaatt cattgtcttt aaaaccttta
    11581 gatataaaga aatctctaac ctttttttta tctgcaacta ttgtattata cgctgttttt
    11641 gcatctggag caacaacatt aaaacttcct gaccaattaa tttcatcaga aacaaaatca
    11701 actgttccta atccagtaac agagatagta tcttcatttg catttctatt tttaacagca
    11761 cttttaaaga taaatgccga cagaattata gcaattgcaa taattaaagc tttaagcaaa
    11821 ttttgattat tttccataat aaatttagtt caataattga ccaaatttat atattatacc
    11881 acaaaaaagt ggttaaaatg tgttatattt cagcatcaat ttgtgaggct aaaatatcct
    11941 ctaaattttc tcttcttctg attaaaatgg ctttaccatc tttaatgaga acttccgccg
    12001 gtcgatacct tgaattgtaa tttgatgaca ttgaaaatcc ataagcacct gcattgtgca
    12061 agcataaaat atcgccttct cgtacctcat ttagttttct gtcccaacca aaggtatcag
    12121 tttcgcaaat ataaccaaca actgtataaa tcctattttt tccttcagga ttagagacat
    12181 tagtgatgtg gtggtaagca tcgtaaaaca ttggtcttat aagatgattt tgaccagaat
    12241 ctacaccagc aaaaacagtg gacatggttt gtttaataac attcacattc acaaaaagaa
    12301 atccagattc tgaaactaag aattttcctg gttcaaaaat taatttcaat tcttttccat
    12361 aagctgtaca aaatgcatta aatcgttcgc taattttttc gcctaattct tcaatatttg
    12421 tagaataatc gtctttacta tattttactt taaatccgct tcctaaatct aaaaattcta
    12481 agtctttaaa atgtttggcc gcatcaaaca ataattctgc accattcaaa aacacatcaa
    12541 catctaaaat atcagaacca gtatgcatgt gcaaaccttc tacttttaaa ttggtagatt
    12601 ttaccacacg ttcgatatgt cttaattgat agacagaaat tccaaatttt gaatcaatat
    12661 gacctgttga aattttagta tttccgccag ccataatgtg tggattgata cgaatacaaa
    12721 taggataggt atctccatat ttatttccaa attgttctaa aatagagata ttatcaatat
    12781 tgatttttac acccaaactt acggctttgg aaatttcttc cattccaaca caatttggtg
    12841 tgtacattat ttcctgaggt gcaaatcctg ccttcaatcc aatttgtact tcttggatag
    12901 aaacagtatc caagttcgca ccaatctttt tcattaattt taaaatattg atattggtta
    12961 atgctttaca agcataatga atttcaacat tcgtagtttt gaaagcattt atcaaacgat
    13021 tataattacg ttctatgaca tttgcatcat aaacatataa aggtgttcca aattctttac
    13081 aaacctctaa aacagataca ccacttatct gatattcatt atttacaatt tccatcttct
    13141 attttaaaaa tgcaaaagta taaataaatc gataaaaact catttgaaaa tcaatattat
    13201 tttagtaaat tttagtagtt ttcataagta cctactacaa tttcggcacc attgtcagat
    13261 ataattgttt ctctgttatt ttggctttct ccaattctgt tgctaaagcc tatagactca
    13321 aaagtatttg ggtcttcttt ttgagttact tcattctgcc ttccaaagtt aaaattcatt
    13381 gtagcattgt ttgcattctc tttaaatcta gcagcttcga tttcctttgc acttggcaaa
    13441 ttatcccaat ttctgcctag agtatcattt ccaacaatca caatctcgct tttggaatta
    13501 tggttattag cacctgaaaa attatagata acaaaaaatg ttacaacagc aatcaaaata
    13561 aatatatgaa caactactct cattaagtct tctatttcaa taaattaacg acaaataaag
    13621 tgccaacttt tgtaaaatta cttaaaatca agcactaaaa ttacttttgt ctgttaatta
    13681 acaccgtgca tccatttttt tacaatcaat gaacttatca taataaaaac tgcagcaata
    13741 actgaaacta atgcaatttg attacatcct gctaaataaa taggtaagga ttgaattgca
    13801 gtaaagacag aaccatcagg gttagcttct ggaatagcca ttttagaacc aatccaacct
    13861 gctaattcat gtccgaatgc acttgccaaa aaccaagtac ccatcataaa tccaataatt
    13921 tttggaggag aaagtttagt tatcatagat aaaccaattg gggaaagaaa aagttcccct
    13981 actgtaataa agaaatagcc taataaaaag tataataaag gcatataacc tatatcgatg
    14041 cctttattac ccccataaac aaaagcataa taaccaatag ctaagaatac aaacgcaaat
    14101 ccaaatttaa gtgggtcatt gggttttaaa tttcgtttat ctaaccaatt ccacataaat
    14161 gcgaaaagag gtgtaaggaa aataatataa aacggattta ttgaattatt gaccatagcc
    14221 gacgacaatt ctgtgcttcc aactttcata tctacatttc gttctgccat taagttcaaa
    14281 gctccacctg cttgttcata aaatgcccaa aaaagtgagg agaaaataat catcaccaag
    14341 gcagcccaga gcatttgttt gggctttatt tcttctttag atgctacaaa taagatatag
    14401 acaattgtta tcaatccgat aatagcaaaa aatatttgag taatatcatt aaacactatc
    14461 attaaaattg ctacaaacac aaacaaaacc gaacctaaat aaattgtttt ctcccaatca
    14521 attccaatta gcgatttttt actcaaaaat gtcgcatcag aaacaccacc ttttccttct
    14581 aatttatctc tatttaataa gaaggtaatt aaaccaatta tcataaatac accagcgatt
    14641 ccgaagccaa gatgccaact tattttttgt cctagataac cacaaatcgc actgcctaaa
    14701 aatccaccga cattgatgcc catgtaaaaa atagcataac ctgcatctct ccttttatca
    14761 ttttcctcat aaaactttcc taaaaaactc gaaatattag gtttaaaaaa tccgtttcca
    14821 actattaaaa atcctaatcc taaaaagaaa gtttggttca ttggaatagc taaaactaaa
    14881 tgaccaatag ccatcattac accgccaaat atgatagatt ttttaaagcc taaaattcta
    14941 tctgctaata ttcctccaaa taatggcgtg atgtaaacca aagcgttata ggcaccatac
    15001 attaaattgc tatctttatc tttgtacaac aatacatttg tcatatataa aatcaacaaa
    15061 gcacgcattc cataaaagga aaagcgttcc cacatttctg taaagaaaag tagaaaaagt
    15121 tgcttaggtt gaatatctct attggttaaa taggtaaaca gaatcattaa tccgcagaaa
    15181 atagatccaa ttaaaattac cattacatct aaactcatat atttattatt attgttcttc
    15241 tgaa
//
LOCUS       DAOQCW010000059        15081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1441_length_15081_cov_5.359710, whole genome shotgun sequence.
ACCESSION   DAOQCW010000059 DAOQCW010000000
VERSION     DAOQCW010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15081
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1441_length_15081_cov_5.359710"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PLS16_06660"
     CDS             complement(<1..205)
                     /locus_tag="PLS16_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60797.1"
                     /translation="MFSLKQIIEQPQLIENISNEQLNDWLKAYPYVSFLHLEKYKRNR
                     TDEQKNKTAFYITHRDVIFKIDYS"
     gene            complement(209..748)
                     /gene="lptE"
                     /locus_tag="PLS16_06665"
     CDS             complement(209..748)
                     /gene="lptE"
                     /locus_tag="PLS16_06665"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HNJ60798.1"
                     /translation="MIVKTLDINVNKKKLSFFALILFALICSNACKIYRFSDASIDPN
                     LKTFTINPTNNIATFQNASAAADFTEKLKEKFIRETRMVLTRQDGDIEYACTIIEYNI
                     EPIALTNTQTLAQNRLNISVKVECINHKDNKKSYTQNFRDGENFSAQTAFNSLESNLQ
                     NTIFDRITQQIFNKSFSNW"
     gene            complement(745..1992)
                     /locus_tag="PLS16_06670"
     CDS             complement(745..1992)
                     /locus_tag="PLS16_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNJ60799.1"
                     /translation="MELQSIKQRFKIIGNSPALNNALDIAVRVASTNLSVLITGESGV
                     GKEVFSQIIHTLSSRKHENFIAVNCGAIPEGTIDSELFGHEKGAFTGAHDKRKGYFET
                     VNNGTIFLDEIGEMPIGTQARLLRLLESGEFIKVGSSNVQKTDVRIIAATNVDLPKLV
                     QIGKFREDLYYRLNTVPIHVPALRERKDDIVLLFRKFASDFAEQYRTPLIQLDDDAKL
                     SLTKYRFPGNVRELKNIAEQVSVLSKDRIIKKEDILRFLPDNELNTLPMLSNGQEASS
                     FSDREILYKMIFDIKNDLNELKKFVWNATNGNVQSSEIDFSDVAIPKISERKNDASHS
                     SNFLHVPDKESADDNPTIIINETPAYEVNTEEQSLSLMDMEKDLIIKALEKHKNKRKD
                     AAFDLGISERTLYRKIKEYNLEK"
     assembly_gap    2201..2300
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2301..6293
                     /locus_tag="PLS16_06675"
     CDS             <2301..6293
                     /locus_tag="PLS16_06675"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNJ60800.1"
                     /translation="TINLKSSPDPQLTEIKNFSRCSGTPNPEELSFTDASTSTNNIKN
                     YKIDWGDGSPAYNSATAPNAVTHTYNPGSYTVTYTITGTGYSCNNATKTYGVLVGKGP
                     TIYPILNQPTQCAPTNYNLPFDVAKMVAENSPSTIYKLYVNDELQSTYDNKSIPLNYS
                     YFFEEGSCGYSSSCPQTSMYSVGVESSNECDFTFFQQCIIVKDSVKPEISGKDTVCVD
                     QANTFMNTDKKSRVFVGTQCTQFADHTWSITPSAGVSANPVFNTPVKKNSISITFSKT
                     GNYRLKLKVDGDCNTKDTFFDIVVVEPIELAATFTPPGCLPESPGYVDVPFTSQIVTG
                     VPKDYKWTVTGGPATFVGGTSASSKDVVVRFPQAGNYKVSLTSSSSTCPVVSWTSPTI
                     VVKTNPKIDTLPLVLGCSTPFTFRNDNGTPLNENDDFYTFYNGNDGTTKYQWTFNSGN
                     PSSASSEKPGNIVYNTAGTFDIITVIRNQCGSDSINREITIYDNIKPTLGKGFDICIS
                     APPIQLPAASPTGGLWSGPGITDVNLGVFNPALAGSGKQTVRYTINQSTACPTFEEME
                     INVIEILGFNAGPNQEVCKGTILKLENDTNFPGGNWTGTGVSSSVLNSFDPYGLAPGT
                     YPVTYTYKDPSGQCSMTAMKNILVKDSIHFTPPPNLCVNVPFEFGSISDNIALPVDWD
                     FGDGSPHAFVLNPTHNYTTSGIKNITLKANTIDGCAATLKFSINVKENAKLNFNVSPA
                     TTCTGEIMVSFPKTHDPASLYTWTYDNKTLVRNDSSAFLLNFSKPILADSIYKIELKA
                     NYFCGSALAEQKVTIKSDPVADFNIQSIGCEPFNPVLTNNAYGSSTTYHWDFGIPGQT
                     SQLPNPTPPTYYNSSRNDTTYTITQTVSNACGTDKISKIITVRANTTYAAITQDKIEG
                     CSPLEVQFESVSSSGSLSWDFGDNTSGFSEFQPKTYTQPGTYIVKLEVQGNCGKSSAT
                     SKVTVHPQPTVDFSVKDLCLGKPTKFTPILTNTAAVTWSFGDGNFSSQMNPSYSYLSA
                     DNFDVKLKAFTNKGCSDSITKIITIANQPKAGFNIDNPSVCLGVPVTLLNASNSAGSP
                     NFTWDFGDGTFDNNAQPEPHNYLHAGTYSVSLLAVEGLCRDSIKKPNAIRVYSLPNAD
                     FTYTIDHLGKFQVPVIFNNISSLATKYVWLFNGKDSSFEKNPTFLFNNLGPNRVILYA
                     ENANGCIDTVIKLLAVDFDGAVFVPNAFAPELGSGESTLFKPKGVDLKEYHVQVFSTY
                     GQLLWESTKLENGQPSEAWDGRYKGEIMPQDVYVWKIRAIFKSGKAWEGNEDINTGKK
                     TTMGSVILLR"
     gene            6369..7454
                     /locus_tag="PLS16_06680"
     CDS             6369..7454
                     /locus_tag="PLS16_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNJ60801.1"
                     /translation="MENITEEKIIYALSQVDDPDFKRDLVSLKMIKDVSFTQEEINFT
                     VELTTPACPLKDQIERDCRNAIAQLVSSTATVNINFTSRVQTAKNVKADVLPNVKNVI
                     AVASGKGGVGKSTVAVNLALALAQTGAKVGLVDADIYGPSIPTMLSLNNMRPNVQKVD
                     DKNYLVPIEYYDMKVMSIGFMIGEKQPVAWRGPMATSALRQMISEVLWEELDYLVLDL
                     PPGTGDIHLTLAQNIGVTAAVVVTTPQEVALADARKAIAFYQLEAINIPILGVIENMS
                     YFTPPDALDKKYYIFGKGGGQLIAQQFEVPFLGELPIEQKIREGGDEGKPIALQFSNS
                     ASTAFANLAQQVAQQAAIINAKVPESV"
     gene            7466..7711
                     /locus_tag="PLS16_06685"
     CDS             7466..7711
                     /locus_tag="PLS16_06685"
                     /inference="COORDINATES: protein motif:HMM:NF013286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNJ60802.1"
                     /translation="MLDTSDILYERVEKALEEIRPFLIADGGDIELVSISEDFIVYIK
                     LKGACESCRMSASTVKAGVESTVKAAVSEIQKVVALN"
     gene            7789..8967
                     /locus_tag="PLS16_06690"
     CDS             7789..8967
                     /locus_tag="PLS16_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNJ60803.1"
                     /translation="MKRKYQHFATNAIRTQHERGFREHSVPLYLTSSFVFEDAEQARA
                     LFADEYTDNIYSRFSNPNTSEFIEKMCILEGMEDGFATATGMAAVFASMMPFLNKGDH
                     ILASSALFASTTKIIKEYMPNWGIEHSFFDINQPETWESLIQPNTKMIFAETPSNPGL
                     VLIDMEHLCNIGKKHNVLVNIDNCFATPYLQQPAKYGADIITHSATKFLDGQGRVTGG
                     VVLANKEITKTIRQFCRQTGPSLSPFNAWILSKSLETLHVRMDRHCQNALELAQWLET
                     QEDIKKVVYPFLKSHPQYELAKKQMTQGGGVVTFEVHGGLERGRKFLDSLQLFSHTAN
                     LGDCRSIATHPASTTHSKIDEASRKASGIENGTIRTSIGLEHIDDIKADILQALEKSR
                     "
     gene            8967..>9268
                     /locus_tag="PLS16_06695"
     CDS             8967..>9268
                     /locus_tag="PLS16_06695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ60804.1"
                     /translation="MISLIIPFFNESKRLDRLEDGLNQYRNRNQLIQEIIFVNDGSSD
                     DTLLHLQKFQKQFPENNIQVIQIETNCGKGNALQAGILQAQHNWVLCNDADLSYRL"
     assembly_gap    9269..9368
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9369..9829
                     /locus_tag="PLS16_06700"
     CDS             <9369..9829
                     /locus_tag="PLS16_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60805.1"
                     /translation="AQHNWVLCNDADLSYRLEQIDEWYETELLDLNKNETIYFGKRII
                     KDKKSKFFVHRIIIGKIFYLLIRLILGINIQDTQCGFKLYPTAIAKEVFKDLQEKRFA
                     FDLEIIYKLIQQNISIQTLPVQCIDIEGSKVNLLKDSWNMFCALFRIRNL"
     gene            9919..10758
                     /locus_tag="PLS16_06705"
     CDS             9919..10758
                     /locus_tag="PLS16_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNJ60806.1"
                     /translation="MQIAELNKTATQVRRDIVRMVHQCKSGHPGGSLGCADFLTALYF
                     DVMKVNTDFDMNGKDEDIFFLSNGHISPLFYSVLARRGFFEVKELATFRHINSRLQGH
                     PTTHEGLPGIRIASGSLGQGISVAAGAALGKKLNKDDKYVFCLTGDGELQEGQNWEAI
                     LFAAANKVDNLIVTVDWNDAQIDGSTQEVCDLGDLEAKFKAFGWEVLTMKGNQMEDVV
                     VQLNEAKNKAKQGKPVVILMRTEMGFGVDYMMGTHKWHGSAPNDEQLAKALAQLEETL
                     GDY"
     gene            11001..12083
                     /locus_tag="PLS16_06710"
     CDS             11001..12083
                     /locus_tag="PLS16_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60807.1"
                     /translation="MLRLLKKIGILITIILGLSVLGSFILPYGWADAVQYAKIQHYKS
                     VKGKYNALFFGGSLEYRQVNPKIVDGVLNSNQIHFNSYNLGIDGHNIIQQLADIDGIM
                     KIKNDSIKYIFVSMSSEPYFFAYNKNTSKWLAWVDLKSTVNSIFILPNAGRTLKEKTR
                     FIILYIRSYFMNLFKVGMVPDVLTHYLGQDTLYQAYIGPDKNGFFAYDDERTYLAQYN
                     RGLDEDLNTSRARFIQDMHYRDSLRNANTQQFSNYKVTDKPNQEQVNMLYRFMKKYNK
                     MGIDVYFILPPRAMTSYSYLLPIFYALPENRRIELADPRQYPQFYTYENGYNFHHLNK
                     KGAAIFSKILGNKIADLLMTSSKIPE"
     gene            12216..13631
                     /locus_tag="PLS16_06715"
     CDS             12216..13631
                     /locus_tag="PLS16_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60808.1"
                     /translation="MSGYFFYGSFAPKYLLILVAITLNDFFAGKKIEDATTKSEKKKW
                     LFLSIAMNLGILIFFKYVNFIDDNIRNLFELLHLPYFVPQTWFDKIIVPVGVSFITFQ
                     SLSYTIDIYKGLIKPERDLGYFATFTAFWPVMVNGPIERAKNLLPQLQEHHSFEYNRV
                     RDGLIRMLFGFFKKLVIADRLAFFVDDMYLHFNDVSGWTIIVGGFLFFFQVYCDFSGY
                     SDIALGAAKIMGINITENFKRPFFSKNLAEFWTRWHVSLSTWLTDYVFFYLGAYKAKG
                     LKVVFNVVFVLAICGLWHGANYPMILAFTMIGIAMAIRYLWQFNIVRNIKPSNAYKLF
                     DKYFPEWGHVLVTVSILVFCFLLFRVHTAQMEWNRMHIDDPVSWWHIAGLLYSKMFHL
                     GSAAYFQEWINHKGIVEFICGIAFLIILLVIEFLIGDKTIEKAISERAKPIRWIIYIL
                     LTLAITWFGVYNNTSFVYFQY"
     gene            13631..14710
                     /locus_tag="PLS16_06720"
     CDS             13631..14710
                     /locus_tag="PLS16_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60809.1"
                     /translation="MRYLLRNIIVLILVVLIASITINIFTPFYWGDYTQTTKILHYKK
                     NPTKYNAIFFGGSLEYRHINPTMVDAIAKQNNIQLSSFNAGIDGHNIIQELRDMEGFL
                     SIKNSELKYIFISLSSEPYFFKPNRNTAKWISWQNFPAYINAMQILPTLDDKWGEKGR
                     FMWYYTTSFIKKNFNMGLLPSLFQSYLDRPSLDSAYLGKNKDGFFPYDDEERLLIEHY
                     KWADEFVIESNAVYKKEKGIRDSLTNSVKHSFDTYNASIKPNQAELNVLVSIIKKYAK
                     KGIDVYFILPPRARTSYSFLLPIYYQLPQERCIELANPNKYPEFYAVEYGYNYHHMNK
                     QGANLYSKLLGERISALINKNKLIP"
     gene            complement(14748..>15081)
                     /locus_tag="PLS16_06725"
     CDS             complement(14748..>15081)
                     /locus_tag="PLS16_06725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNJ60810.1"
                     /translation="NNIFLVGNSIGGISSYILDTSKISANKISVKRNAINIYPNPTKN
                     TATINLKTFDEVQNLSCFNLMGQPFKIGYQQAGKLLNISVEALPSGLYILQFQQKNEI
                     YYAKIMKE"
BASE COUNT         5014 a         2254 c         2513 g         5100 t
ORIGIN      
        1 tactataatc tatcttaaaa atgacatctc tatgtgtaat ataaaaagct gttttgtttt
       61 tctgctcatc cgttctgttt cttttatact tttccaaatg aagaaatgaa acataaggat
      121 aggcttttaa ccaatcattt aattgttcat tagaaatatt ttctattaac tggggttgtt
      181 cgatgatttg tttaagtgag aacatagttt accaattaga gaaagattta ttaaaaattt
      241 gctgggtaat tctatcaaag atagtgtttt gtaaattaga ttctaacgaa ttaaatgcag
      301 tttgtgcaga aaaattttcg ccatctctaa aattttgtgt gtaagacttt ttattatctt
      361 tatgattaat gcattccact tttacactaa tatttaatct attttgtgct aaagtttgcg
      421 tgttggttag agcaattggt tcaatattat attcaataat agtacaagca tattcgatat
      481 ctccgtcttg tctagttaaa accattctcg tttctcgaat aaatttttct tttagttttt
      541 cagtaaaatc tgctgcagca ctcgcatttt gaaaagttgc aatattattt gtaggattaa
      601 ttgtaaatgt ttttaaatta ggatcaatac tcgcatcaga aaaacgatat atcttacacg
      661 cattagaaca aattaaagca aatagaatta aggcaaaaaa actaagtttt tttttattta
      721 cgtttatatc taacgtcttt acaatcattt ttctaaatta tattctttaa tttttctata
      781 taatgttctt tctgaaatgc ctaaatcaaa tgcagcatct ttccttttat ttttgtgttt
      841 ctctaaagct tttataatca aatctttttc catatccatc aatgaaagac tttgttcttc
      901 cgtattgact tcataagctg gggtttcatt gataattatt gtcggattat catcagcaga
      961 ttctttatca ggaacatgca agaaatttga ggaatgtgat gcatcatttt ttctttcgct
     1021 tattttagga atagcaacat cactaaaatc aatttctgaa ctctgaacat ttccatttgt
     1081 agcattccag acaaacttct ttaattcatt caaatcattc tttatatcaa aaatcatttt
     1141 atataaaatc tctctatctg aaaaactaga cgcttcttgc ccattgctta acattggcaa
     1201 ggtattgagt tcattatctg gcaaaaaacg caatatatct tcctttttta ttattctatc
     1261 tttagataaa acagaaactt gctctgctat attttttagt tctcgaacat taccaggaaa
     1321 tcgatatttt gtcaatgaca atttagcatc atcatctaat tgtatcaatg gcgttcgata
     1381 ttgttctgca aaatcagagg caaattttct aaacaacaat acaatatcat ctttcctttc
     1441 tcttaaagct ggaacatgga ttggaactgt atttaaacga taatataaat cttctcgaaa
     1501 ttttcctatt tgaactaatt tcggcaagtc aacattggta gctgcaataa ttcgaacatc
     1561 agttttttga acatttgaag atccaacttt aataaattct ccactttcta ataaacgtaa
     1621 caatcgggct tgtgttccaa tgggcatttc tcctatttca tctaaaaata tagtgccatt
     1681 atttactgtt tcaaaatagc cttttctttt atcgtgagca ccagtgaacg cacctttctc
     1741 atgcccaaac aattcagaat ctattgtgcc ttcaggaatt gcaccacaat tgacggcaat
     1801 aaaattttcg tgcttacgag aggaaagtgt atggataatt tgtgaaaata cttctttgcc
     1861 gacaccactt tctccagtta ttagcacaga taaatttgtt gatgctactc gtacagcaat
     1921 atccaatgca ttatttaatg ctggtgagtt tccaataatc ttaaaccttt gttttatgct
     1981 ttgtaattcc aaattttacg agatatttat attagaatac aaatataatg atttatatga
     2041 atagtagaat attaagcagt acttagaaat attgtgaatt atctgctaaa aacttgacaa
     2101 atagcatata atgaagactc aataaaataa gtaatccttt tttttattaa ttttgggtta
     2161 tgagaataaa aaatctaaaa ggaatctttt tgattctaat nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn actatcaatc taaaaagttc tccagaccca caacttacag
     2341 aaataaaaaa ttttagcaga tgttcaggca caccaaatcc agaagaatta tcttttacgg
     2401 acgcaagtac ttcaactaat aatattaaaa attataagat agattggggt gatggctctc
     2461 cagcatataa ttccgctaca gctccaaatg ccgttacgca tacctataat ccaggttctt
     2521 atacagtaac ctatacaatt acaggaactg gttattcttg taataatgcc actaaaactt
     2581 atggcgtttt ggttgggaaa ggaccaacca tatatccaat tctaaatcag ccaacacaat
     2641 gtgctccaac aaattacaac ctgccttttg atgttgcaaa aatggttgcg gaaaatagcc
     2701 caagtactat ttacaaatta tatgtaaatg atgaattaca atctacttat gataacaaaa
     2761 gtattcctct aaattattcc tatttttttg aagaaggtag ttgtggctac tcctcttctt
     2821 gtccacaaac gagtatgtat tctgtaggag tggaaagttc aaatgaatgt gattttactt
     2881 tctttcaaca atgtatcata gtaaaagatt ctgtaaaacc tgaaattagt ggcaaagata
     2941 ccgtttgtgt tgatcaggct aatacattta tgaatacaga taaaaaaagc cgagtatttg
     3001 tgggtactca atgtacacaa tttgctgacc atacttggtc gataactcca agtgctggtg
     3061 tttctgcaaa tcctgtattt aatacacctg ttaagaaaaa cagtatctcg attacatttt
     3121 ctaagacagg aaactataga ttaaaattaa aggttgatgg tgattgtaat acaaaagata
     3181 cattttttga cattgttgtt gtagaaccaa ttgaattggc tgcaacattt acacctccag
     3241 gatgtctccc agaaagccca ggatatgttg atgttccctt taccagtcaa attgttactg
     3301 gtgtacctaa agactataaa tggacggtaa ctggtggacc agccactttt gttggtggca
     3361 cgagtgcaag ttctaaagat gttgttgtac gtttccctca agctggaaat tataaagtta
     3421 gtcttacctc gagttcttct acttgtccag tcgtttcttg gacaagtcca acgatagtcg
     3481 ttaaaactaa cccaaaaatt gacactttac ctttagtttt aggatgctca acacctttta
     3541 cttttaggaa tgacaatgga actccattaa atgaaaatga tgatttctat actttctata
     3601 atggaaacga tggaacaaca aaatatcaat ggacattcaa tagcggaaat ccttcttctg
     3661 cgtcatcaga aaaacctgga aatatcgttt ataataccgc aggtactttt gatattataa
     3721 cagtgataag aaaccaatgt ggaagcgata gtatcaatag agaaattacg atttatgaca
     3781 atataaaacc aactttaggg aagggttttg atatctgcat aagtgctcca ccaattcaat
     3841 tacctgctgc tagtccaaca ggtggcttat ggtcaggacc cggaattacc gatgtaaatt
     3901 taggggtatt taatccagca ttggcaggca gtggaaaaca aactgttcgc tataccatta
     3961 atcagagtac agcatgtcct acttttgagg aaatggaaat taatgtcatt gaaattttag
     4021 gatttaatgc tggaccaaat caagaagttt gtaaaggaac aatattaaaa ttagaaaatg
     4081 acactaactt ccctggtgga aattggactg gaactggtgt ctcgagttct gttctcaatt
     4141 catttgaccc ttatggatta gcacctggaa catatccagt aacttatact tataaagatc
     4201 ctagtgggca atgttcaatg actgctatga aaaatatctt ggtaaaagat agtattcatt
     4261 ttacgccacc tcctaattta tgtgtaaatg tcccatttga atttggtagt atttcagata
     4321 atattgcatt gccagtcgat tgggattttg gagatggaag tccacatgca tttgtactca
     4381 atccaacgca taattatacg acaagtggaa taaaaaatat aactctaaaa gccaatacca
     4441 ttgatggatg tgcagccaca ttaaagtttt ctatcaatgt aaaagaaaat gcaaaattaa
     4501 actttaatgt ttcgccagct acaacatgta ctggagaaat aatggtttcg tttcctaaaa
     4561 ctcatgaccc tgcaagtcta tatacttgga cttatgataa taaaacatta gtaagaaatg
     4621 actcttctgc attcttatta aatttttcta aaccaatatt agcagattct atttataaaa
     4681 ttgaattaaa agcaaattac ttctgtggtt ctgctttagc agagcaaaag gtaaccataa
     4741 aatcagatcc tgttgcagat tttaatatcc agtctattgg ttgtgagcct tttaatccag
     4801 tattgactaa caatgcttat ggaagctcta ctacttatca ttgggatttt gggattcctg
     4861 gacaaacttc tcaattacca aatcctactc ctccaactta ttataattcg agtagaaatg
     4921 atacgaccta taccataacg caaacagttt ctaatgcgtg tggtactgat aaaatttcaa
     4981 aaataattac agttcgtgca aatactacct acgctgcaat tactcaagat aagatagaag
     5041 gctgctctcc tttagaagtc caatttgaaa gtgtatcctc aagtggtagt ttaagttggg
     5101 attttggcga caatacttct ggattttctg aattccaacc aaagacctat acacaacctg
     5161 gaacttacat tgtgaaacta gaagttcaag ggaattgtgg taaatcaagt gctactagta
     5221 aggttaccgt tcatccacaa cctacagttg actttagtgt aaaagattta tgtttaggta
     5281 aaccaactaa atttacacca attctaacaa atactgctgc agtaacatgg agctttggag
     5341 atggcaattt ctcttctcaa atgaatccaa gctattcata tctatcagct gataattttg
     5401 atgtcaagtt gaaagcattt acaaacaaag gttgtagcga ctcaataaca aaaataataa
     5461 cgattgccaa tcaacctaaa gctggattta atattgataa tccgagtgta tgtttaggtg
     5521 tacccgttac tttattaaat gcatccaact cagcaggcag ccctaatttt acttgggatt
     5581 ttggagatgg cacttttgat aataatgcac agcccgaacc tcataattat ttgcatgctg
     5641 gcacctattc tgtttcatta ttggcagtag aaggtctttg tagggatagt attaaaaaac
     5701 caaatgcaat acgtgtgtat tctttaccaa atgctgattt tacttatacg attgaccatt
     5761 taggaaaatt ccaagtacct gttattttta ataacatttc ttctttagct actaaatatg
     5821 tttggttatt caatggaaaa gattcctcgt ttgaaaaaaa tccgactttc ctatttaata
     5881 atttaggacc taatcgagtt atattatatg ccgaaaatgc aaatggttgt attgatactg
     5941 taatcaaatt acttgctgta gattttgatg gagctgtatt tgttcctaat gcttttgctc
     6001 cagaattagg tagtggcgaa tctactttat ttaaaccaaa aggcgttgat ttgaaagaat
     6061 atcatgttca agtattttca acttatggac aattactttg ggaaagtaca aagttggaaa
     6121 atggacaacc ttctgaagct tgggatggga gatacaaagg agaaataatg cctcaagatg
     6181 tttatgtttg gaaaattaga gccattttta aaagcggaaa agcttgggaa ggcaacgagg
     6241 atattaatac tggtaaaaaa acaaccatgg gttctgttat tttattaagg taaattcttt
     6301 gttatttaaa gtaaatctaa acaagctttt gctttatttg ttattttctt tagtacattt
     6361 gccttattat ggagaatatt acagaagaaa aaataattta tgcactttcg caagtagatg
     6421 atccagattt taaaagagat ttggtttcat taaaaatgat aaaagatgta tcatttactc
     6481 aagaagagat aaattttaca gttgaactta caaccccagc atgtccactg aaagatcaaa
     6541 ttgagagaga ttgcagaaat gctattgctc aattggtttc gagtacagct acggtaaata
     6601 taaatttcac atcaagagta caaactgcta agaatgtaaa agcagatgta ttgccaaacg
     6661 taaaaaacgt cattgctgtt gcttcgggaa aaggtggtgt tggaaaatca acggttgccg
     6721 taaacttagc tttggcactg gctcaaactg gagcgaaagt aggtttagta gatgcagata
     6781 tctatggacc atctattcca acaatgttga gcctaaacaa tatgcgtccc aatgttcaaa
     6841 aagtagatga taaaaactat ttggtgccga ttgaatatta tgatatgaaa gtaatgtcaa
     6901 ttggatttat gattggcgaa aagcaacctg ttgcttggcg tggtccaatg gctacttctg
     6961 ctttaagaca aatgatttct gaagtacttt gggaagagtt agattattta gttttagatt
     7021 tacctccagg aactggagat atacatttaa ctctagctca aaatattggt gtaaccgcag
     7081 ctgtagtggt taccacacct caagaagttg cattagctga tgctagaaaa gcaattgcat
     7141 tttatcaatt agaagcaatc aatattccaa tattaggcgt tattgaaaac atgtcttatt
     7201 tcacacctcc tgatgctttg gataaaaagt attatatttt tggtaaaggc ggtggacaat
     7261 taattgcaca acaatttgaa gtgccattct taggtgaatt accaattgaa caaaaaatta
     7321 gagaaggcgg tgatgaagga aagcctatcg ctcttcaatt ttcaaatagt gcaagtactg
     7381 catttgctaa cttagctcaa caagtcgctc aacaagcagc tattattaat gcaaaagtac
     7441 cagaatcagt ataatttcag atacgatgtt agatacgagt gatatattat atgaacgtgt
     7501 tgaaaaagca ttagaagaaa ttcgcccttt tttaatagca gatggtggag atattgaatt
     7561 ggtaagtatt tcagaagatt ttattgttta tataaaatta aaaggtgcat gtgaatcttg
     7621 tagaatgagt gcttctactg ttaaagctgg agtcgaaagc accgtaaaag ctgcggtttc
     7681 tgaaatacaa aaagttgtag ctttgaatta ggaattttga attgaccaaa aaatttaaac
     7741 tttccttatt tttcaactta ctatctttac tttttaaatt gctatccaat gaaaagaaaa
     7801 tatcagcatt ttgctacaaa tgctatccgt acacaacacg aacgtggatt tagagagcat
     7861 agtgttccat tatacctaac ttcttctttt gtttttgaag atgctgagca agctagggca
     7921 ttatttgcag atgaatatac tgataatatt tattcacgat tttcaaatcc aaatacttct
     7981 gaatttatag aaaagatgtg cattttagaa ggaatggaag atggctttgc taccgcaaca
     8041 ggtatggctg ctgtttttgc ttctatgatg ccgtttttaa ataaaggcga ccatattttg
     8101 gcttcatcag ctttatttgc ttctactaca aaaattataa aagaatatat gcctaattgg
     8161 ggcatagaac atagcttttt tgatattaat caacctgaaa cttgggaaag tcttattcaa
     8221 ccaaatacaa aaatgatatt tgcagaaacg ccttccaatc ctggtttagt attgattgat
     8281 atggaacatc tttgcaacat tgggaaaaag cataatgttt tagtgaatat tgataattgc
     8341 tttgcaacgc cttatttaca acagcctgct aaatatggtg ccgatatcat tacccattca
     8401 gctacaaaat ttttagatgg gcaaggtcga gttactggtg gcgttgtttt ggccaataaa
     8461 gaaatcacta aaacaataag acaattttgc agacaaactg gtccttcttt atctccgttt
     8521 aatgcttgga tattaagcaa aagcttagaa accttgcatg ttagaatgga tagacattgt
     8581 caaaatgctt tagaattagc tcaatggtta gaaacacaag aggatattaa aaaagtagtt
     8641 tatccatttt taaaatctca tcctcaatat gaattagcta aaaaacaaat gacacaaggc
     8701 ggtggcgttg ttacctttga agtccatggt ggtttagaaa gaggacgaaa attcttagat
     8761 agtttgcaac ttttctcgca tactgcaaat ttaggtgatt gtcgctctat tgcgacacat
     8821 cctgcatcaa caacacatag taaaattgat gaagcatcaa gaaaagcttc tggcattgag
     8881 aatggaacta tccgaacttc aataggtttg gagcatattg acgatataaa ggcagatata
     8941 ctacaagcat tagaaaaaag tagataatga tttcattgat tattccattt tttaatgaat
     9001 ctaaaagatt ggatagatta gaagatggtt taaatcaata taggaataga aatcaactta
     9061 tacaggaaat tatttttgtg aatgatggaa gttctgacga cacactactg catttacaaa
     9121 agtttcaaaa gcaatttcct gaaaataaca ttcaagttat tcagatagaa acaaattgtg
     9181 gcaaaggcaa tgcattacaa gcagggattt tacaagcaca acacaattgg gttttgtgta
     9241 atgatgcgga cttatcttat cgtttagann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnaa gcacaacaca attgggtttt gtgtaatgat gcggacttat cttatcgttt
     9421 agagcaaata gacgaatggt atgagactga attattagac ttgaacaaaa atgaaacaat
     9481 ttattttgga aagcgaatta taaaggataa gaaaagcaaa ttctttgtcc atagaattat
     9541 tattggaaaa atattttacc tacttattag gttgatttta ggtataaaca ttcaagatac
     9601 acaatgtggt tttaaattgt atccaactgc tattgcaaaa gaagtattta aagatttgca
     9661 agaaaaacgg tttgcctttg atttggaaat catttataaa ttaatacaac aaaatattag
     9721 cattcaaact ttacctgttc aatgcattga tattgaaggt tctaaagtta atttacttaa
     9781 agatagctgg aatatgtttt gtgcattgtt ccgaattaga aatctttaaa taaaatcctc
     9841 attatattta tatccatttc ttcattttta attttctcta aactttattt ttaatacttt
     9901 tgtaattcaa ttaaaaaaat gcaaatagca gaactcaata aaacagcaac acaagttaga
     9961 agagatatcg ttcgtatggt acatcagtgc aaatctgggc atcctggagg ttctttaggt
    10021 tgtgccgatt tcttaactgc attatatttt gatgtaatga aagtgaatac ggattttgat
    10081 atgaatggaa aagatgaaga tattttcttt ttatccaacg gtcatatttc tccattgttt
    10141 tattctgttt tggctcgtag aggttttttt gaagtaaaag aattggcaac attcagacat
    10201 atcaattcca gattgcaagg acacccaacc acacacgagg gactacccgg aattagaata
    10261 gcatcaggaa gcttaggtca aggaatttct gttgcagctg gtgcagcatt aggcaaaaaa
    10321 ctgaataaag atgacaaata tgtattttgt ttaactggtg atggagaatt acaagaagga
    10381 caaaattggg aagcaatttt atttgctgcc gcaaataaag tggacaatct aattgttaca
    10441 gtcgattgga atgatgctca aattgatggt tctacacaag aagtttgtga tttgggcgat
    10501 ttagaagcaa agtttaaagc atttggttgg gaagttttaa ctatgaaagg caaccaaatg
    10561 gaagatgtcg ttgtacaact aaatgaagca aaaaataaag caaaacaagg caaaccagtt
    10621 gttatcttga tgcgtacaga aatgggcttt ggcgtggatt acatgatggg cactcataaa
    10681 tggcacggct cagcacctaa tgatgaacaa ttagcaaaag ccttagctca attagaagaa
    10741 actttaggag attattaaaa ttattttcat taattcttat gttttttgat agcttatact
    10801 tcttaatgct atctaagaaa ttgtctgaat caagatttgc aggattaaat gatgaacagg
    10861 atttttaaat acaatcctaa aatatagaaa attctgatgc tgacaaaaaa agtacaaaca
    10921 tacactccta ctaaattttg tgcttaataa aacccaaata aaaaaacata tttttcgtat
    10981 tttagcctga ttttaatgta atgttgaggc tgctaaaaaa aataggaatt ttaattacaa
    11041 tcatcttggg tttgtccgtt ttgggcagtt tcattttgcc ttatggttgg gctgatgctg
    11101 tacaatatgc taaaattcaa cattataaat ctgttaaagg aaaatacaat gccttatttt
    11161 ttggtggcag tttggaatat cgacaagtca atccaaagat tgtcgatggt gttttaaata
    11221 gcaatcagat acattttaat agttataatt taggtattga tggccataat attatccaac
    11281 aactagcgga tattgatggt attatgaaaa taaaaaacga cagtatcaaa tatatctttg
    11341 taagtatgtc gagtgaacct tatttttttg cttacaataa aaatacttca aaatggttgg
    11401 cttgggtaga tttaaaatcc actgtaaatt ccatttttat attacctaat gctggaagaa
    11461 ccctaaaaga gaaaacgaga tttattatat tgtatatccg aagttacttt atgaatttat
    11521 ttaaagtagg tatggttcca gatgttttga ctcattattt aggtcaagat acactttatc
    11581 aagcttatat tggaccagac aaaaatggtt tttttgctta tgatgatgag cgaacttatt
    11641 tggcacaata caatcgaggt ttagatgaag atttgaatac atcaagagct agatttatac
    11701 aagatatgca ctatcgtgat agtttgcgaa atgcgaatac ccaacaattc tctaactata
    11761 aagttacaga caaaccaaat caagaacaag tgaatatgtt gtatcggttt atgaaaaaat
    11821 acaataaaat gggtatagat gtgtatttta ttttacctcc acgagcaatg acctcctatt
    11881 cttatttatt acccatcttc tatgcattgc cagaaaatag gcgaattgag ttggcagacc
    11941 caagacaata cccacaattt tatacgtatg aaaatggcta taattttcat catttgaata
    12001 aaaaaggagc tgccattttt agtaagatat tgggtaacaa gattgcagat ttattaatga
    12061 cctcctcaaa aatacccgaa taatttatta tttttaacaa attaaattga tggacaaaca
    12121 tgctttttaa ctcttttagt ttccttattt tctttccaat atttctgtta ctttatttca
    12181 gaattaatgc caaacatcaa tggaaactat tgttattgag tggttacttt ttttatggtt
    12241 cttttgctcc aaaatatttg ttgattttgg tggctattac tttgaatgat ttttttgctg
    12301 gtaaaaaaat tgaagatgca acaaccaaat cagaaaaaaa gaaatggttg tttttgagca
    12361 ttgctatgaa tttgggtatt ttaattttct ttaagtatgt aaatttcata gacgataata
    12421 ttcgcaacct atttgagtta ttgcatctgc cctattttgt tccacaaact tggtttgata
    12481 aaattattgt gcctgtcggt gtttcattta taactttcca atctctcagt tacacgattg
    12541 atatttataa aggcttgatt aaaccggaac gagatttggg ttattttgca acttttactg
    12601 cattttggcc tgtaatggtc aatggtccaa ttgaacgagc taaaaactta ctaccgcaat
    12661 tacaagaaca tcattcattt gagtataata gagttcgaga tggtttaata agaatgttgt
    12721 ttggattttt taaaaaactc gtcattgccg accgattagc tttttttgtt gatgatatgt
    12781 atttacattt taatgatgta agtggttgga caattattgt cggtggcttt ttgtttttct
    12841 ttcaggtata ttgtgatttt agtggctatt ctgatattgc tttaggagct gcaaagatta
    12901 tgggtattaa tatcacagaa aatttcaaac ggcctttttt ctccaagaat ttagctgaat
    12961 tttggacacg ctggcacgtt tctctatcaa catggctgac agattatgtg ttcttttatt
    13021 tgggtgcata taaagcaaaa gggctaaaag tcgtctttaa tgttgttttt gttttggcta
    13081 tttgtggctt gtggcacggt gctaattatc cgatgatatt agcatttaca atgataggca
    13141 ttgcgatggc aattagatat ttgtggcaat ttaatatcgt taggaatata aaacctagta
    13201 atgcttataa actctttgat aaatactttc cagaatgggg tcatgtttta gtaaccgtta
    13261 gcatcttagt tttctgtttt ttattattca gggtacatac ggcacaaatg gaatggaatc
    13321 gaatgcatat tgatgaccca gtgagttggt ggcatattgc aggtttattg tattcaaaaa
    13381 tgtttcattt aggttctgca gcttattttc aagaatggat taatcacaaa ggaattgttg
    13441 agtttatttg tggcattgct tttcttatta ttttattagt aattgaattt ttgattggcg
    13501 acaaaactat tgaaaaagcg atttcagaaa gagcaaaacc catacgatgg attatctata
    13561 ttttgctcac tttggcgata acttggtttg gggtttataa taatacttca tttgtttatt
    13621 ttcaatacta atgcggtact tgttgagaaa tattattgtc ttgatattgg tggtattaat
    13681 agcttcaata acgataaata tttttacacc tttttattgg ggagattata cacaaacaac
    13741 taagatttta cattataaga agaacccaac aaaatacaat gcgattttct ttggtggcag
    13801 tttagagtat agacatatca atcctactat ggttgatgct attgcaaagc aaaataatat
    13861 tcaattatca tcattcaatg caggaataga tggtcataat attattcaag aattaagaga
    13921 tatggaagga ttcttatcta ttaaaaattc tgagcttaaa tatattttta tcagtttatc
    13981 tagtgaaccc tattttttca aacctaatag aaatacagcg aaatggattt cctggcaaaa
    14041 ctttcctgct tacattaatg cgatgcaaat cttaccgact ttagatgata aatggggcga
    14101 aaaaggtcga tttatgtggt attataccac ttcatttatt aaaaagaatt ttaatatggg
    14161 cttgttacct agtttgtttc aaagttactt agacagaccc tctcttgatt cagcttattt
    14221 aggtaaaaat aaagatggtt tttttcctta tgatgatgaa gagcgtttgt tgatagagca
    14281 ttacaaatgg gcagatgaat ttgtcattga atctaatgca gtttataaaa aagaaaaagg
    14341 tataagagat agtttgacca atagcgtaaa acattcattt gatacgtata atgcaagcat
    14401 aaaacccaac caagctgagt tgaatgttct tgtcagtatc atcaaaaaat atgctaaaaa
    14461 aggaattgat gtttatttta ttttgcctcc acgtgctaga acaagctatt catttttatt
    14521 gccaatttat tatcaacttc cacaagaaag atgcattgaa ttagcgaatc caaacaaata
    14581 tccagaattc tatgcagtgg aatatggata taattatcac cacatgaata aacaaggtgc
    14641 aaatttatat agtaagttat taggcgaacg aattagtgca ttaattaata aaaacaaact
    14701 tattccttaa tagctattga caaagtatca agcaaataaa atgcttttta ttccttcatt
    14761 attttagcat aatatatttc attcttttgc tggaattgca aaatatatag tcctgatggc
    14821 aatgcttcaa cactaatatt taatagcttt ccagcttgtt ggtatccaat tttgaaaggt
    14881 tgtcccatta aattgaaaca acttaaattt tgaacttcat cgaatgtttt taaatttatt
    14941 gtagccgtat tttttgttgg atttggatag atattaatag catttctttt tactgaaatt
    15001 ttattcgctg aaatcttaga cgtatctaaa atataagaag aaatgccacc aattgaattg
    15061 ccaactagaa atatattatt c
//
LOCUS       DAOQCW010000060        15043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1447_length_15043_cov_5.624700, whole genome shotgun sequence.
ACCESSION   DAOQCW010000060 DAOQCW010000000
VERSION     DAOQCW010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15043
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1447_length_15043_cov_5.624700"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="PLS16_06730"
     CDS             complement(<1..827)
                     /locus_tag="PLS16_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60811.1"
                     /translation="MKSAIKIFLTLAFFISIFNTYAASDVTLTINYSTVGTKSGFDHI
                     SKLIVFVDGINKGESNEQKQTIPNSVTIKIPYGSHSIKAVFYAKQGNYWEERTLKNNY
                     SFDCIYNKTLNFNKNTTIDLTFDFNNNEVKVKEPVFNNNTTSNNKVGNYQASLQKIND
                     YLKTFDNGYYGYLELKDGYLYDRFKSGKYCKVLMTDLDKAIEYTTSEKVIVKCKTGKC
                     VYSTYTDAYHEELSFSQSAYFNSKELVTLINNFIADYNNKPSTNNSITNSNNIDYQSA
                     "
     gene            complement(996..2579)
                     /locus_tag="PLS16_06735"
     CDS             complement(996..2579)
                     /locus_tag="PLS16_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HNJ60812.1"
                     /translation="MQRRRFLGLSTLASASLFGWSTFSLSSCKKNTATKASTNYNAIV
                     IGSGFGGSVTALRLAEKGINTLMIEMGKFYDVTQSNNTFSPTLPPDKRSTWLKTKTEL
                     PFGINLSWKEKYTGVLDRVAFKNMAVYRNICLGGGSISNGGVLLEPQETHFNQFISST
                     INFQELKNKYFPIVRNMMQASKMPDDLFNSKYYQFAQVCKEHAINAGFEITNADSFYN
                     FDIWRKETLNLVPKSALNGELLYGNNNGIKNSLDRNYISDALGTGNLTIKTLSKVVEV
                     SYQAETEKYTVIVETINERGILQETTTHQCSHLFVCAGSLGTSEILLKARETGKLPNL
                     NQEVGNAFGTNGNAFTIRKNINKPTGNMHSSPPTLSVLDFNNPIAPLAAMQDIFPLGI
                     DLRILLMVGQPYNEYKGSFQYQQSTNSIELNWATNGMDQPKQAMQNFIEKMNTANNSQ
                     LDTTFIKEGVSSTFTYHPLGGVVLDKASDNFGRLKGYKKLYAIDGSMLPGNCAMVNPS
                     LTIAALAERNLREILNNDF"
     gene            complement(2657..3346)
                     /locus_tag="PLS16_06740"
     CDS             complement(2657..3346)
                     /locus_tag="PLS16_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMUG2 DNA glycosylase family protein"
                     /protein_id="HNJ60813.1"
                     /translation="MSKTIADKVIQFNKNLDYKGSLPDGFRVLNPYLDNPETLQVMQA
                     FYHKFYNDNQKRKFIIGINPSRHGAGVTGVPFTDTKRLESICGIAMKTAYTHEVSSVF
                     LYDMIQEYGGINRFYQNFYINSPFPLAIVRKANGNNWVNANYYDDPKLFDAVKDYMID
                     SLKQHFAIGLDNSKVFILGKKNATFIQKLNQEAKLFEQFSVLEHPRYIQQYKSKEKQL
                     YIDKYIIELNK"
     gene            complement(3350..6637)
                     /locus_tag="PLS16_06745"
     CDS             complement(3350..6637)
                     /locus_tag="PLS16_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60814.1"
                     /translation="MSNYQLLIHRLDAFIRKYYLNQLLRGSLIFLSSILAYYLVASVL
                     EYNLYFSTSVRKIILLAFIGLSFLLFIKLIVIPLSNYLKLGQVISHENAAKIIGNHFE
                     NIKDKLLNILQLHQQNQNVQNKELIEASINQKIENIKLVPFTSAINLSENKKYLPYLL
                     PILLLILGIILLAPQVIKESNERLITPNTVYAPKAPFDFILENKNLKTIQFGDIEIKL
                     KTKGEKLPNEVYLISGKQKIKLEKLSKNEFSHRLTNLQSDTKFYFSANEFNSNEYNIQ
                     VFKKPIITNHSVKIIYPSYTGKKSEILKDVSDILIPIGTKVTWTILADNTDNIQIFSE
                     NSVTNAQNASKHIFSFSKQILQDTKFKLLPSNSTIKNPDTLFYSITTIPDNPPMIQCQ
                     QIIDSTQPDFILMSGSISDDYALSKFEFVYRIFDEKDQLIIGKRRNLSIPQPSISTFD
                     YPFNIKEIELNPGQRLEYFMEVWDNDAIHGAKSSKSQTFQYKKMSVAELENQENKNNE
                     AINDGLSNLQDKTNKLSKQIEAFKQKILSKNSLSWEDKKAAEQLRNEQQQLLKEVQQM
                     DKKYEDNLNNQKEFKKENEEILQKQEKIQEMLKDLMNDELKDLMQQIEDLMSKMQQKN
                     AFENLDKMQMSNQNLKKELEKMQELFKQLQLEQKAQETIDKLNKLAEEQKQLSEKQNE
                     SNSSLKAKQDELNINFENIKENIKQLEQLNKDLDDKMKLDQPEKDAQQIEQNMDESSK
                     QLQENQKQNANQKQKKAAEQMKNLAQKMQNSLDQMQMDQNAEDIQLIRKLLDNLLKFS
                     FEQEKTMQDLKKTDPQSPKYVQIMQKQANLKEDADMIADSLQSLGKRQFQLQNFISDE
                     IYKLNRELKKALDNLQERYIASASSAQQFVMTAANNLALMLSESLDNLQMQQKQKMSG
                     DGNCKKPGKEGQKPSMNQMQKELGEQLKNMQQQMQQGKEGQKMSKELAEAAEKQAMIR
                     EMLRKMKEQMSQEEKDKVGVDDLLNKMDDIEKDIITKNISNKTINRQKEIETRLLEYD
                     KAKREQGEEEKRQSKSAPDIQKQLPPALQEYLQKKKSATEMYQSIPPNLQPFYKNLVD
                     KYFQLLQ"
     gene            complement(6760..7320)
                     /locus_tag="PLS16_06750"
     CDS             complement(6760..7320)
                     /locus_tag="PLS16_06750"
                     /inference="COORDINATES: protein motif:HMM:NF027363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HNJ60815.1"
                     /translation="MKQILFLLVAFFIFDASAQKTLNGVTLPAKVKANNQELVLNGGG
                     IRKKAMFKVYVLGLYTPAKTKDAKAIINTNDDLMARLVITSSVVNSSNMSEAIIEGFE
                     KSTNNNMAPLKSKIDAFIGAFKEEIKTGDVFDLVYSPSTGTKALKNGKLVTTVEGEAF
                     KKALFGIWLGDNPIDTKVKSGILGLE"
     gene            complement(7399..9027)
                     /locus_tag="PLS16_06755"
     CDS             complement(7399..9027)
                     /locus_tag="PLS16_06755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ60816.1"
                     /translation="MVNYNVQYFLEQCLNSIFRSSVSFNFEVYVVDNASLDGSVDMLK
                     KQFPQVKLIENKENVGFAKANNQAIKLSQSEYVLLINPDTIIQEDTLEKCVAKMANDS
                     KIGALGIKMYDGNGKYLPESKRGFPSPIAALSKMSGLSKIFPKSKLFGKYHLGYLDKN
                     QNHEVDVLSGAFMLVRKSVFDTIGLLDESYFMYGEDIDLSYRIQQAGYKNYYFAESNI
                     IHFKGESTKKGNLNYIKLFYGAMIIFASKQLNKTQASFLIPLLKIGIILRAILEIIEQ
                     FLSKILYPLIDLGLMLFNLLTLKYIFEQYIKSDEHLTYPSTFMYINIPIYMSLWLLSL
                     WIIGAYTKNAKWKHIFIGLIIGFLSISIVYSFLPLSLRSSRSIIVLGFLLNTITLLIY
                     KWIVAQVFQTNNNFFNTQEHYILVCEQEECIHLQKLVSKTQPNNHYVGYVTVQDIMKK
                     DKNYLGKTIQIKDILEVYPISLIYFSTDSIAMQIIVETMQKTTNKSIHFKMIVKNKYI
                     ISSNNKNIQGEIVGFEIPRNQKISILQKIRNWLV"
     gene            complement(9058..9675)
                     /gene="recR"
                     /locus_tag="PLS16_06760"
     CDS             complement(9058..9675)
                     /gene="recR"
                     /locus_tag="PLS16_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNJ60817.1"
                     /translation="MIFPSKLIEDAVGAFAQFPGIGKKTALRLVMHLLQNDTETTKNF
                     AETLLKMRQNIKHCNNCHNVSDNDICSICNNDFRDHHTICVVENFRDILAIEQTQQYN
                     GVYHVLEGLISPLDGIGPEQLKIETLENRIKNQEITEIIMALNPTIDGDTTIFYLSKK
                     FKDYPIKITTISRGIAFGGELEYVDEITLARSIHARLPYENYLGK"
     gene            9859..10827
                     /locus_tag="PLS16_06765"
     CDS             9859..10827
                     /locus_tag="PLS16_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNJ60818.1"
                     /translation="MKYIVSFLCLVLAFACVPKKEYDSLLNKYMQTQVDKSNVDKDFA
                     DYQKLSAENKAKLDASLEKLKNDSTELSKLIDNMKNEMDNLLSEKERIEKENQEKLKD
                     ALSQNQKTNAELLQMQLDLEKQKLTLDQKQKDLDKLQSEIALKSNRINDLEKTLNEQK
                     MQSEKLKNMITDALVKFNAGELEVYTKDGKVYVSMSDKLLFKSGSTIVETKGIEALGK
                     LADVIKKNPDILVNIEGHTDNVPYSSSAGCIKDNWDLSLMRASSVLHILTEKYFVSRL
                     QLTASGKGEFSPKATNITPEGKAQNRRTEIILTPKIDKIMELLNDK"
     gene            10889..11830
                     /locus_tag="PLS16_06770"
     CDS             10889..11830
                     /locus_tag="PLS16_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60819.1"
                     /translation="MKKIAFVLVLLVSTIFAHSQNIDGLWRGTIIMYVYDVYMAEMNL
                     KKMPNETFTANLKITNGFYKGEFNISGNVCNRVNLEITAIILLKENESSNWIDCLNGT
                     LDLSEGENILSFTDTWHQTLEMQKNYAACKVKFIQKDMFQCLRSAYLVKQNYDIYTNA
                     FDDLWNKHDRLEQEAPQKNKETYIVEKKEEKEPIIEKKLPFKNRDVVVKSEIQVSNKE
                     ITIEYWDKYNFDGDSISLYLNDRPILENVLLTKIKSSVVVQLDKKNNYLTLHALNLGK
                     EPPNTAAITVKDGKKIQNIMLYSDLEKSGTLKIVLKE"
     gene            11934..12182
                     /locus_tag="PLS16_06775"
     CDS             11934..12182
                     /locus_tag="PLS16_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HNJ60820.1"
                     /translation="MKKDTHPQNYRTVVFKDFSCDHTFLGKSCAVTKDTFTWEDGNEY
                     PLVKLEISNMSHPYYTGKAKFVDTAGRIDKFNKKYAKK"
     gene            12249..13433
                     /locus_tag="PLS16_06780"
     CDS             12249..13433
                     /locus_tag="PLS16_06780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HNJ60821.1"
                     /translation="MSLNIVLFDTPQDWANLLPLTATRAIADCKIGILTIKDKWRNLL
                     QTEPEILTQAYLQVKYKHIPNIGTIYVNAKVLPSANLLAHLEKLQLNGALVSNEVLVA
                     FRSSELIQNNQTISQLKFSKQETIEHLVMINYPWDIFLNNGNEIKKDIQLLDLKPNPE
                     ALSATNILIGKDIYVGKNVRCEASVLNSNDGPIYLDDDTEVMEGCTVRAPFYLGKHST
                     LKMQAKIYGDTSIGEHCKIGGEVSNSVIFGYSNKAHDGFLGNSVLAEWCNLGADTNNS
                     NLKNNYGKIKVWNYPTQDYINSGLQFSGLIMGDHSKAAINTQFNTGTVVGVCANVFDA
                     GFSAKFIPDFSWGRNAEFALETAYQVTQRVMERRHLQLSEIDKSILKHIFETTKLFRK
                     FN"
     gene            complement(13430..14053)
                     /locus_tag="PLS16_06785"
     CDS             complement(13430..14053)
                     /locus_tag="PLS16_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60822.1"
                     /translation="MKKIIYFAVLALLTSTSFGQEHEPVKEYKFKFKAGVASTLHFYK
                     QHENEIGYKKVALGNGLGTELGYYITKKISVNLHYYLHYYKNPIKNSGAPFLSNKFHV
                     NQQNHIIATTVGFTVFQKKSSSIIINTGPGFTIKNEQVALLDPLTKIPTRIIDNKSFK
                     YVQPISINIENFIKNKIILGAGIQTFIFGEKQPLKGACLFSYLGLPF"
     gene            complement(14125..>15043)
                     /locus_tag="PLS16_06790"
     CDS             complement(14125..>15043)
                     /locus_tag="PLS16_06790"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60823.1"
                     /translation="LSSSPSSSASSFASSSFSEAKEQMEQIIDNLKTIYKGLNNEAEK
                     IEYSPERLEELQDKYNTYNRLMQKHQVKDITALQEIKKSYEEKINQYNHHDELLIQIN
                     DEINVLKNVLQKQAQKLSKNRISKIPDFEQSLSTILQQIGMPFAQVKFEVITGNLNEK
                     GIDTIRFLFSPNKGVAVQSLQQVGSGGEKSRLMLAIKSLSVQAKASPTMIFDEIDTGI
                     SGEVANKVALLLKEIGKNHQIIAITHLPQVAAKAEHHYFVYKKHDKAVTYTNIKELNK
                     EERIQEIAIMLSGENPNDAAIANAKILMQ"
BASE COUNT         4917 a         2126 c         2254 g         5746 t
ORIGIN      
        1 gcagattgat aatctatatt atttgaatta gtaatagaat tatttgtgct tggtttattg
       61 ttataatcag caatgaaatt attgattaag gtgactaatt cttttgaatt aaaataggca
      121 ctctgtgaaa acgataattc ttcgtggtac gcatcagtat aagttgaata gacacatttt
      181 cctgttttac atttaactat gactttttca ctagttgtat attcgattgc tttatctaaa
      241 tctgtcatta atactttaca atattttcct gatttgaatc tatcatacaa gtaaccatct
      301 ttcaattcaa gataaccata gtaaccatta tcaaaagttt ttaaatagtc atttattttt
      361 tgaagagatg cctgatagtt gccaacttta ttattgctag ttgtattatt attaaatact
      421 ggttctttaa ctttaacctc gttgttatta aagtcgaatg ttaaatcgat ggttgtgttc
      481 ttattaaaat tgagcgtctt gttataaata caatcaaaag aataattgtt ttttagtgtc
      541 cgttcttccc agtaattacc ttgttttgca taaaatacag ctttaataga atgacttcca
      601 taaggtattt ttatcgttac actattagga atagtttgct tctgttcatt actttctcct
      661 ttattaatac catctacaaa cactatcaac ttagaaatgt gatcaaagcc tgactttgta
      721 cctacagttg aataattaat ggttaatgta acatctgatg ctgcataagt attgaaaata
      781 cttataaaaa atgctagagt taaaaatatt ttaatggctg atttcatatt ctttatttta
      841 tagttttttt attgcaattt atattatttt aatggttaat caatacttta aatattaaat
      901 tttattggtt ttaattatta aatttaacta gcattttaca aaaatagtac caagaattat
      961 ttgaaaaagt aagctatttg ttagattatc aatatttaaa aatcattatt taggatttct
     1021 cttaaattgc gttctgctaa agctgcaatt gtcaaagaag gattgaccat agcacagttt
     1081 cctggtaaca tggaaccgtc gatggcatat agttttttat aacctttcag tcttccaaaa
     1141 ttatctgaag ctttgtctaa tacaacgcca cccaatggat gatatgtaaa tgtacttgaa
     1201 acgccttctt tgataaaagt tgtatccaat tgactattgt ttgcagtatt cattttttca
     1261 ataaaatttt gcatggcttg tttgggttga tccattccat ttgtagccca atttaattca
     1321 atggaatttg tactttgttg atattgaaaa ctgcctttat attcattgta tggctgtcca
     1381 accataagca aaattcttaa atctatacca agtggaaaaa tatcttgcat ggctgccaat
     1441 ggtgcaattg gattattgaa atctaaaact gaaagtgttg gcggacttga atgcatattg
     1501 cctgttggct tattaatatt ttttctaatc gtaaatgcat taccgtttgt gccaaatgca
     1561 tttcccactt cttgatttaa gttgggtaat tttccagttt ctctggcttt taataatatt
     1621 tcagacgtac ctaagcttcc agcacaaaca aatagatgag aacattgatg tgttgttgtt
     1681 tcttgtaaaa tgcctctttc attaattgtc tcaacaataa cggtatattt ttcagtttct
     1741 gcttgatagc taacttcaac aacttttgat aaggttttaa tcgttaagtt tccagtacct
     1801 aatgcatctg aaatataatt tctatctaat gagtttttaa ttccattatt attgccataa
     1861 agcaattcgc catttaaggc agattttgga actaagttta gtgtttcctt acgccaaata
     1921 tcaaaattat aaaatgagtc tgcatttgta atttcaaaac cagcatttat tgcatgttcc
     1981 ttacaaactt gtgcaaattg ataatattta gaattaaata aatcatcagg catttttgat
     2041 gcttgcatca tattcctaac tattggaaaa tatttatttt ttagctcttg gaaattaatt
     2101 gtggaagaaa taaattggtt gaaatgtgtt tcttgtggct ctaacaatac tccgccatta
     2161 gaaatagaac cgccacccaa acaaatatta cgataaactg ccatattttt aaaggctact
     2221 ctgtccaaaa cgccagtata tttttctttc cacgaaagat tgataccaaa aggtaattca
     2281 gtttttgttt ttaaccaagt tgagcgtttg tcgggtggga gcgtaggtga aaaggtattg
     2341 ttactttggg ttacatcata aaattttccc atttcaatca tcaacgtatt gatgcctttt
     2401 tctgccaaac gaagtgcagt aactgaacca ccaaaacctg agcctataac aatggcatta
     2461 tagtttgtgc tagcttttgt cgctgtattt tttttacaag aggataagga aaaagtactc
     2521 cacccaaata atgatgcaga ggctaaagta gataatccca aaaatcttcg acgttgcata
     2581 gcttaaaatc aattttttat tgaatgtaag atactatttt gaaatgaaat cgtgttgttt
     2641 tttgcaattt tgtaaactat ttattcaatt caatgatgta tttatcaata tacaattgtt
     2701 tttctttgga tttatattgt tgaatatatc ttggatgttc taataccgaa aattgctcaa
     2761 ataattttgc ttcttgattg agtttttgaa taaatgtagc atttttcttt cctaaaataa
     2821 acactttgga attatctaaa ccaatagcaa aatgctgttt caatgaatct atcatatagt
     2881 cttttacggc atcaaataat tttgggtcat catagtaatt agcatttacc caattatttc
     2941 catttgcttt tcgtacaatg gctaatggaa atggtgagtt gatataaaaa ttttgataga
     3001 accgatttat accaccatat tcttgtatca tatcatacaa aaaaacagaa gaaacttcat
     3061 gcgtatatgc agttttcata gcaataccac aaatgctttc caatcgtttt gtatctgtaa
     3121 atggcacacc tgttacacct gctccgtgcc gacttggatt gataccaatg ataaattttc
     3181 gtttttgatt gtcattgtaa aatttatgat agaatgcttg cattacctgc aatgtttctg
     3241 gattatctaa atatgggttg agtacacgaa atccatcggg caaacttcct ttgtaatcta
     3301 agtttttatt gaattgaata actttatctg caattgtttt ggacatattc tattgcaata
     3361 attggaaata tttatctact aaatttttgt agaatggttg gagatttggc ggaatagatt
     3421 gatacatttc tgttgcagat tttttctttt gtaaatattc ttgcaaagct ggtggtaatt
     3481 gtttttgaat atctggagct gatttagatt gtcgtttttc ttcctcgcct tgttctcttt
     3541 ttgccttgtc gtattctaat aatcgagttt caatttcttt ttgtctattg atagttttgt
     3601 tgctaatatt tttagtaatg atgtcttttt ctatatcatc catcttattc aataaatcat
     3661 caacaccaac tttgtctttt tcctcctgcg acatttgttc tttcatttta cggagcattt
     3721 ctcgtatcat ggcttgtttt tcagcagctt cagccaattc cttactcatt ttctgtcctt
     3781 ctttgccttg ctgcatttgt tgttgcatat ttttcaattg ttcgcctagt tctttttgca
     3841 tttgattcat actcggtttt tgaccttctt tacctggttt tttgcaattg ccatctccac
     3901 tcattttctg tttttgttgc atttgtaaat tatccaaaga ttcactcaac attaatgcta
     3961 aattgtttgc agcagtcatt acaaattgct gtgcagatga ggcagaagca atatatcttt
     4021 cttgtaaatt atctaaagct ttttttagct cacgattgag tttatatatt tcatctgaga
     4081 tgaagttttg caactgaaat tgtctttttc ctaaagattg aaggctatct gcaatcatgt
     4141 cggcatcttc tttcaaatta gcttgttttt gcataatttg aacatatttt ggagactgcg
     4201 gatcggtctt tttcaagtct tgcattgtct tttcttgctc gaaagaaaat tttaataaat
     4261 tatctaataa ctttctaatg agttgtatgt cttctgcatt ttgatccatt tgcatttgat
     4321 ctaaactatt ttgcattttt tgagctaaat ttttcatttg ctctgctgct ttcttttgtt
     4381 tttggttggc attttgtttt tgattttctt gcaattgttt agaactttca tccatatttt
     4441 gttctatctg ttgggcatct ttttctggtt ggtctaactt cattttatca tccaaatcct
     4501 tattcaattg ttctaattgc tttatatttt ctttgatgtt ttcaaagttt atattgagtt
     4561 catcttgctt tgctttcaat gatgaattgg attcattttg tttttcagaa agttgctttt
     4621 gttcttcagc aagtttatta agtttatcta ttgtttcttg tgctttttgc tctaattgta
     4681 attgtttaaa aagttcttgc attttttcca attccttttt cagattttgg ttgctcattt
     4741 gcattttatc taaattttca aatgcatttt tttgttgcat tttgctcatc aagtcttcaa
     4801 tttgttgcat taaatctttt aattcatcat tcatcaaatc tttcagcatt tcttgaattt
     4861 tttcttgctt ttgcaaaatt tcttcattct ctttcttaaa ttctttttga ttatttagat
     4921 tgtcttcata ttttttatcc atttgttgca cttccttcaa aagttgttgt tgctcatttc
     4981 gtaattgttc tgctgctttt ttatcttccc aacttaaact attttttgat aatatttttt
     5041 gtttgaaagc ttcgatttgt ttacttaatt tattggtttt gtcttgcaaa ttggatagac
     5101 catcattaat agcttcatta tttttatttt cttgattttc taattctgca acactcattt
     5161 tcttatattg gaatgtttgc gatttggaag acttagctcc atgtatggca tcattatccc
     5221 aaacttccat aaaatattct aatcgttgcc ctggatttaa ttcaatttct ttgatattaa
     5281 atggataatc aaatgttgaa atcgatggtt gaggaatact taaattccgt ctttttccaa
     5341 taattaattg gtccttttca tcaaaaattc tataaacaaa ttcaaattta cttaatgcat
     5401 aatcatcgga aatactacca ctcattaata taaaatctgg ttgtgtgcta tcaatgattt
     5461 gctgacattg tatcattggt ggattatctg gaattgtagt tatagaataa aatagtgtat
     5521 ctgggttttt tatggttgaa ttggaaggaa gcaatttaaa ctttgtgtct tgtaaaattt
     5581 gtttggagaa actaaatata tgcttacttg cattttgtgc atttgtaaca ctattttcgc
     5641 taaatatttg aatattatcg gtgttgtcag ctagtatggt ccaagtaact ttggttccga
     5701 ttggaattaa aatatcacta acatctttta atatttctga tttttttcct gtataacttg
     5761 gatatattat tttgactgaa tggtttgtaa taattggctt tttgaaaacc tgaatattgt
     5821 attcattgga attaaattcg ttggcagaaa aataaaactt cgtatcagat tgtagatttg
     5881 taaggcgatg gctaaattca tttttagata atttctctaa tttgattttc tgttttccag
     5941 atattaagta aacttcatta ggcaattttt ctccttttgt ttttaactta atttcaatat
     6001 cgccaaactg tattgttttt agatttttat tttctaatat aaaatcaaat ggtgcttttg
     6061 gagcataaac cgtattgggt gtaattaagc gttcattact ttctttaata acttgtggag
     6121 caagtaaaat aattcctaaa atcaataata aaatgggtaa taagtagggt agatattttt
     6181 tattttctga aagattaata gcagacgtga atggtactaa ttttatattt tctatctttt
     6241 gattgatact ggcttcaatc aattctttgt tctgtacatt ttgattttgt tgatgtaatt
     6301 gtaaaatatt taataattta tctttgatat tttcaaaatg gttaccaata atttttgcag
     6361 cattttcatg tgaaataact tgacctaatt ttagataatt tgaaagtgga ataacaatca
     6421 gtttgatgaa tagtaaaaat gataatccaa taaaagcaag taaaattatt tttcttactg
     6481 atgtagaaaa atataaattg tactctagta cacttgcaac taaataataa gctaaaatgc
     6541 ttgataaaaa tatcaaactt cctctcaata attgattgag ataatacttt cggatgaaag
     6601 catctaaacg atgaattaat agctgataat tactcattag tcttgtgtac taaattagtt
     6661 aagtgtactt taaaaaacta aactattttg ttatttttct taaaataaaa aactacccaa
     6721 tttatgggta gtttaaataa tattttagcg cttttatgct tattctaaac ctaaaatacc
     6781 tgatttaact tttgtatcaa ttggattatc acctaaccaa atgccaaata aagctttttt
     6841 gaatgcttcg ccttcaacag tagtaactaa tttgccattt tttaaagctt tagtaccagt
     6901 tgatggacta taaactaaat caaatacatc gcctgtttta atttcttctt tgaacgcacc
     6961 tataaatgca tcaatttttg attttaatgg agccatatta ttattagttg atttctcaaa
     7021 accttcaata atagcttcag acatattgct actatttaca actgaagatg taatcactaa
     7081 tcttgccatt aaatcatcgt ttgtattaat gatggctttt gcatctttag tttttgcagg
     7141 tgtataaagt cctaaaacat aaactttgaa catcgctttt tttctaattc caccgccgtt
     7201 aagcactaat tcttgattgt tcgcttttac tttagcagga agtgtaacac catttaatgt
     7261 tttttgtgca cttgcatcga aaatgaaaaa tgcaactaat aagaaaagaa tttgtttcat
     7321 gtcaatttat ttatttataa aactacaaaa accttaccaa aaataaaaag gaatttttaa
     7381 ttgttttttt tgaagagttt aaaccagcca atttctaatc ttttgtaaaa ttgaaatttt
     7441 ttggtttcta ggaatctcaa agccaacaat ttcgccttga atgtttttat tattgctact
     7501 gatgatatat ttatttttta caatcatctt gaaatgtata gatttatttg ttgttttttg
     7561 catagtttct acgatgattt gcatagcaat actatccgta gaaaaatata ttagagaaat
     7621 aggatatact tcaagaatgt cttttatttg tatagtttta cctaaataat ttttatcttt
     7681 tttcattata tcttgtactg taacatagcc cacataatga ttgttgggtt gagttttact
     7741 tactaatttt tgtaaatgaa tacactcttc ttgctcgcat actaaaatat aatgctcttg
     7801 agtgttgaaa aagttattgt tcgtttgaaa tacttgagca actatccatt tataaatgag
     7861 taaagtgatt gtattaagta aaaatcctaa tacaatgatg ctacgtgagg agcgaagcga
     7921 taaaggtaaa aatgaataaa ctatagaaat agataaaaat cctatgatta atccaataaa
     7981 aatatgtttc cattttgcat tttttgtata agcaccaata atccacaaac tcaagagcca
     8041 caaactcata tagattggaa tattgatata cataaaagta cttgggtaag taagatgctc
     8101 atcggatttt atatattgct caaaaatata ttttagtgtt agaagattaa aaagcatcaa
     8161 acctaaatct atcaaagggt ataaaatttt agataaaaac tgttcaataa tttctaaaat
     8221 tgctcgtaaa ataataccaa ttttaagaag aggaattaga aaacttgctt gtgttttatt
     8281 gagctgtttt gacgcaaaaa taatcattgc accataaaac aatttgatat aatttaaatt
     8341 gccttttttc gtgctttcac ctttaaaatg tatgatatta ctttcagcaa aataatagtt
     8401 tttatagcct gcttgttgga ttctgtacga taaatctata tcttctccat acataaaata
     8461 actttcgtcc aataagccaa tggtatcaaa aacagatttt ctgactaaca taaatgcacc
     8521 actcaataca tcaacttcat gattttgatt tttatctaaa tagcctaaat gatatttgcc
     8581 aaataatttt gattttggaa atattttaga caagccactc atcttggaga gtgctgctat
     8641 tggagacgga aaacctcttt ttgattcagg taaatatttg ccattgccat catacatttt
     8701 gatacctaat gctccaattt ttgaatcatt agccattttt gcaacacatt tttctaaagt
     8761 atcttcttgt attattgtat caggattaat caataaaaca tattcagatt gacttaattt
     8821 tatagcttgg ttgttggctt tggcaaatcc gacgttttct ttattttcaa ttagttttac
     8881 ttgaggaaat tgctttttta gcatatccac cgaaccatct aaagaagcat tatctacgac
     8941 ataaacttca aagttaaatg aaacggaaga acgaaaaatg ctgttcagac attgttctaa
     9001 aaaatactgt acgttataat ttactatgac aatactgagt ttgatagtag tgtttattta
     9061 ttttcctaga taattttcat aaggcaatct tgcgtgaata cttctcgcta atgtaatttc
     9121 atcaacatat tccaattctc caccaaaagc aattcctctt gatatggttg tgatttttat
     9181 aggataatct ttgaattttt tagataaata gaaaatagta gtatcgccat ctatcgttgg
     9241 atttaatgcc attattattt ctgtaatttc ttgattttta attctgtttt ctaaagtttc
     9301 aatctttaat tgttcaggtc caataccatc taatggcgaa attaagcctt ctaaaacatg
     9361 atagacacca ttgtattgtt gcgtttgttc tatagccaaa atatctctaa aattttcaac
     9421 aacacatatt gtatgatggt ctctaaaatc gttgttgcat atactacaaa tatcattgtc
     9481 agaaacatta tgacaattat tgcaatgttt tatattttga cgcattttta aaagcgtttc
     9541 tgcaaagttt tttgttgttt cagtatcatt ttgaagtaaa tgcatcacca atcgtaaagc
     9601 agtttttttt ccaattccag ggaattgagc aaaagcaccc acagcatctt caatcaattt
     9661 tgatggaaat atcatgtttc aaaagtacga tattaaggtt atttaatcaa gaaaaaccaa
     9721 attatattgg gttttagaaa tatacaaaat tttatttaaa aatgtataat ctgatttttg
     9781 actctgtttt cacaccctgt gagtattttg atgattatat attgataatt gcacttactt
     9841 ttacccaaaa tgtacaaaat gaaatatata gtgagttttc tttgcttggt attagctttt
     9901 gcttgtgtac ctaagaaaga atatgatagt ttgttgaata aatacatgca aactcaggtt
     9961 gataaaagta atgtagataa ggattttgca gattaccaaa aattatcagc agagaataaa
    10021 gcaaaattag atgcttcttt agaaaaatta aaaaatgatt ctacagaatt atccaaattg
    10081 attgataaca tgaaaaatga aatggataat ttgttgtctg aaaaagagcg tatagaaaag
    10141 gaaaatcaag agaaattaaa agatgcatta tctcaaaatc aaaagacaaa tgctgaatta
    10201 ttgcaaatgc aattggattt agaaaaacaa aaattgactt tagaccaaaa acaaaaagat
    10261 ttagataaac ttcaatcgga aattgcacta aaaagcaata gaatcaatga tttggaaaaa
    10321 acgctgaacg aacaaaaaat gcaatctgag aaattaaaaa acatgattac agatgcttta
    10381 gtgaaattta atgctggcga actagaagtt tatactaaag atggaaaagt ttatgtttct
    10441 atgtctgata aattattatt caaatcagga agtactattg ttgaaacaaa aggtattgag
    10501 gcattaggaa aattagcaga cgtaatcaag aaaaatccag atattttagt caatatcgaa
    10561 ggacatactg ataatgttcc ttactcaagc agtgcaggtt gtataaaaga taattgggat
    10621 ttaagtttaa tgcgtgcaag tagtgttttg catattctga ccgaaaagta ttttgtaagt
    10681 aggttgcaat tgacagcaag tggcaaaggc gaattttcac ccaaagcaac taacattaca
    10741 ccagaaggga aagcacaaaa tagaagaact gaaattatct taacaccaaa aatagataag
    10801 ataatggaat tactgaatga caaataactt caaatgttaa taaaaatagg gtttgcctaa
    10861 attttctatc tttgcagaaa atagagcgtt gaagaagata gcatttgtcc ttgttttatt
    10921 ggtttctacc atatttgcac acagtcaaaa tattgatgga ttgtggcgag gtacaattat
    10981 catgtatgtt tatgatgtat atatggcaga aatgaatctg aaaaaaatgc caaatgaaac
    11041 attcactgct aacctcaaaa ttacaaatgg tttttataaa ggcgaattta atattagtgg
    11101 aaatgtttgt aatcgagtaa atctagaaat tacagctatt atattattga aagaaaatga
    11161 atcttctaat tggattgatt gtttgaatgg tactttggat ttaagcgaag gggagaatat
    11221 tttatctttt acagatactt ggcatcaaac tttggagatg cagaagaatt atgctgcttg
    11281 taaagtaaaa tttattcaaa aagatatgtt tcaatgcttg cgttctgctt atttagtcaa
    11341 acaaaattat gatatttata cgaatgcatt tgatgattta tggaataagc acgacagatt
    11401 agaacaagaa gccccacaaa aaaataagga aacctatatc gttgaaaaaa aggaagagaa
    11461 agaaccaatc attgagaaaa aattgccttt taaaaatcgt gatgtggtgg taaaaagtga
    11521 aattcaagtg tctaataaag aaattactat agaatactgg gataaatata attttgatgg
    11581 tgattctata agtttgtatt tgaatgatag acctatcctt gaaaacgtct tgctgaccaa
    11641 aattaaatca tctgttgttg ttcaattgga taaaaagaat aattatctga ctttacatgc
    11701 cttgaattta ggtaaagaac cacccaatac agctgcaatt actgtaaaag atggcaaaaa
    11761 gatacagaat atcatgttgt actcagattt agaaaagagc ggtactttga aaattgtatt
    11821 gaaagaatag agcagtaata taaaattaac ataatttgaa gcttttaata ttgcatattt
    11881 tagtttttta gaaaataaat gtatatcttt gcactttcaa aatagaatta gatatgaaaa
    11941 aagacacaca tccacaaaat tatagaacag tagtattcaa agatttttct tgcgaccaca
    12001 ctttcttggg taagtcttgt gcagttacaa aagatacatt tacttgggaa gatggaaacg
    12061 aatacccatt agtaaaatta gagatttcaa atatgtctca tccttactat acaggaaaag
    12121 ctaaatttgt agatactgca ggtcgtattg ataaattcaa caaaaaatac gctaaaaaat
    12181 aattttacga ttaaagatta taacattaat gctccgaata ttcggagcat ttcttatttt
    12241 tacccattat gtcattgaat attgtactgt ttgatacgcc acaagattgg gcaaacttat
    12301 tgccgctcac tgccacacgt gctattgctg attgtaaaat tggaatttta accataaaag
    12361 ataagtggag aaatttatta caaactgaac cagaaattct tactcaagct tatctacaag
    12421 taaaatataa acatatccca aatattggaa caatttatgt caatgcaaaa gtattgccaa
    12481 gtgctaattt gttggcacat ttagaaaagc tacaattaaa tggagcatta gtgtctaatg
    12541 aagttttagt ggcttttaga agttcagagc ttattcaaaa taaccaaact ataagtcaat
    12601 tgaaattttc taaacaagaa acaattgaac acttagtgat gataaattat ccttgggata
    12661 tatttctaaa taatggaaat gagattaaaa aggatattca attattggat ttaaaaccaa
    12721 atccagaagc actttctgcg accaatattt tgattggaaa ggatatttat gttggcaaaa
    12781 acgtacgttg tgaagcatct gttttgaata gtaatgatgg cccaatttat ttagatgatg
    12841 ataccgaagt gatggaaggt tgcaccgttc gagctccttt ttatttagga aaacatagta
    12901 ctttaaaaat gcaagctaaa atttatggcg ataccagtat tggtgaacat tgtaaaattg
    12961 gcggggaagt ttctaatagt gtaatttttg gatattccaa taaagcccac gatggatttt
    13021 taggtaattc ggttttggca gaatggtgca atttgggtgc tgataccaat aattcaaacc
    13081 tgaaaaataa ttatggaaaa ataaaagttt ggaattatcc aacacaagat tatatcaatt
    13141 ctggattgca attttctggc ttgattatgg gcgaccattc taaagctgca atcaatacac
    13201 aattcaatac aggaacggtt gttggtgtat gtgcaaatgt atttgatgct ggtttttctg
    13261 caaaatttat tcctgatttt tcttggggaa gaaatgcaga atttgcttta gaaactgcgt
    13321 atcaagtgac ccaacgtgtg atggaaagac gacatttgca attatctgaa atagataaaa
    13381 gtattttaaa acatattttt gaaacaacaa agttgtttag aaaattcaat tagaaaggta
    13441 agcctaaata agaaaataaa cacgcacctt taagtggttg tttttctcca aatataaatg
    13501 tttggatacc agcacctaat atgattttat tcttgataaa attctcaata ttaatagaaa
    13561 taggttgtac gtatttaaaa gacttattat caatgatacg tgttggaatt ttagttaatg
    13621 gatctaaaag tgccacttgt tcatttttaa tggtaaaacc tggaccagta ttgataataa
    13681 tagagctgct tttcttttgg aaaactgtaa atccaactgt tgttgcaata atatgatttt
    13741 gttggttcac atggaattta ttagataaaa aaggtgctcc actattttta ataggattct
    13801 tgtaataatg caaataataa tgcaagttta cacttatctt ttttgtgata taataaccta
    13861 attcagtacc taaaccatta ccaagagcta cttttttgta acctatttca ttttcatgtt
    13921 gtttataaaa gtgtaaggta cttgctacac cagctttgaa tttgaattta tattccttaa
    13981 ctggctcatg ttcttgtcca aatgaagtgc tggttaataa agccaaaact gcgaaataga
    14041 ttattttttt cattgctcga agataagctt tttttatatt aatccttaaa attggttaat
    14101 tattttctat gattttgagg ctaattattg catcaatatt ttggcatttg caatagcagc
    14161 gtcattcgga ttttcgccac tcaacataat tgcaatttcc tgaatacgtt cctctttatt
    14221 aagttcttta atatttgtat aggtaactgc cttatcatgt tttttgtaaa caaaataatg
    14281 atgttctgct tttgctgcta cttgtggcaa atgtgtgatt gcaataattt gatggttttt
    14341 tccaatctct tttaataata aggctacttt gtttgctact tcaccagaaa tacctgtatc
    14401 tatttcatca aaaatcatag ttggggaagc ttttgcttgt acagataatg atttaattgc
    14461 caacattaac cttgattttt caccaccaga acctacttgt tgcaaggatt gtacagctac
    14521 acctttatta ggagaaaata aaaaacgtat cgtatctatg cctttttcat tgaggtttcc
    14581 tgtaattact tcaaatttta cttgtgcaaa aggcattccg atttgttgta aaattgtact
    14641 taaagattgc tcaaaatcag gaatttttga aattctattt ttagataatt tttgagcctg
    14701 tttttgtaat acatttttaa ggacatttat ttcatcattg atttgaataa gcaattcatc
    14761 gtgatgattg tattgattga ttttttcttc gtaagatttt ttaatttctt gtaacgctgt
    14821 aatgtctttt acttgatgtt tttgcatcaa acgattgtaa gtattatatt tatcttgtaa
    14881 ctcttctaat ctttctggag aatattctat tttttcagct tcattattca aacctttata
    14941 gatagttttt aggttatcta taatttgttc catttgttct tttgcttctg aaaatgaaga
    15001 ggaagcaaac gaagaggctg aagaagaagg tgaagaagaa aga
//
LOCUS       DAOQCW010000061        14891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1474_length_14891_cov_5.168374, whole genome shotgun sequence.
ACCESSION   DAOQCW010000061 DAOQCW010000000
VERSION     DAOQCW010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14891
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1474_length_14891_cov_5.168374"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1588)
                     /locus_tag="PLS16_06795"
     CDS             complement(<1..1588)
                     /locus_tag="PLS16_06795"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNJ60824.1"
                     /translation="MLKTFNINLGGQAFVINEDAYHVLDNYLNNIKRIYANEEGKEEI
                     LQDIETRFAELFIQKGKDRIVAVEDVNQVIAVMGTPEDFEDGEQAETTTNTKPNFQKS
                     TPTEKRLYRDKDNSVVAGVCSGLTAYFGINDPIWLRIIFIISPFITVGTALLVYIILW
                     IIMPEAKTTAEKLAMKGEPINLSNIVEKKNEKIEELTSSSSLNNIVNAIGKIVSILFN
                     ILIKLSKVLLYIIFGSIILSLIGTVIAFIVSVFIGTPIAKQFLFDANTNSPILLILGG
                     LLVGVSALVGLILLLVHLFSKNYKVFNLKTILPIVGLIISGLFILSFVTKEGAAFFKH
                     KEKIVQSVPLNYSAMGDTLKLDISPMTKREHQININSFGDLMSFLGKDFEIENDVDIQ
                     IFTTPLDSFYVEKEFSSFGKTEKEALKNATSISHQLTQMNNKLIIDPLFSFNTKERKI
                     RNQKLIVRVYVPENKVIQWTPQTEEYIDISDMPINWDKETYRKVNANNQVKIDIKSDS
                     SNSVNINIRGNDDDDYDDDYD"
     gene            complement(1618..1968)
                     /locus_tag="PLS16_06800"
     CDS             complement(1618..1968)
                     /locus_tag="PLS16_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNJ60825.1"
                     /translation="MAAVENQFSIENTKVQMRKGILEFCILGIISRGEVYASDILDEL
                     KSANMLVVEGTVYPLLTRLKNLGLLAYEWKESNSGPPRKYYVLTEQGRGALDELQFTW
                     LELANAVERTITKK"
     gene            complement(2029..2475)
                     /locus_tag="PLS16_06805"
     CDS             complement(2029..2475)
                     /locus_tag="PLS16_06805"
                     /inference="COORDINATES: protein motif:HMM:NF022838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-like domain-containing protein"
                     /protein_id="HNJ60826.1"
                     /translation="MKTTSILKGTMVAVLLSANFIVSAGDRVIPYSETPSSVKTYINT
                     HFPNYKVLKVETDNDILYKEYEVFLSNGIKVEFDGKEKSIKNISGQSKLPESIIPKKV
                     RDYVNKNYSGNTIVEYNLENNGISVELSNDIELKFSTNGDFLRIDD"
     gene            complement(2505..2780)
                     /locus_tag="PLS16_06810"
     CDS             complement(2505..2780)
                     /locus_tag="PLS16_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60827.1"
                     /translation="MKKVLTFGMAVMMISLLSYSAWFAFNKYEANKNKETTVQYVVDE
                     KGNPTVTYIENNVSPRKLLKFIDTIEAFEFTNLSGVMQVFTVKVKLN"
     gene            2998..3828
                     /locus_tag="PLS16_06815"
     CDS             2998..3828
                     /locus_tag="PLS16_06815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HNJ60828.1"
                     /translation="MKKTSILIYFTISIIIFSACKTSKITTTQNTLSEQRKLNDIALN
                     GKVYASIWQQNAGEFRALCYQAFNIAKNNIDQKISSNPTRPLAIITDIDETFLDNSPY
                     AVTQALQGKEFSYSTWLEWTSLGKAIAYPGSLDFFNYAASKNVTVFYITNRNQNDKPG
                     TLKNLQDLHFPFADNEHLIVMDKTSNKEQRRQAIQKNYDVFMYLGDNLGDFSDVFYKK
                     TQTERNKNTDDLADEFGNKFIVLPNSGYGDWETALPGFNFDLTPAQKDSVFLKNLIGY
                     "
     gene            3893..5206
                     /locus_tag="PLS16_06820"
     CDS             3893..5206
                     /locus_tag="PLS16_06820"
                     /inference="COORDINATES: protein motif:HMM:NF001923.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HNJ60829.1"
                     /translation="MSKWKFERTNNSLPEVYSSISVTQGKNKWNKFLAFLGPGLMVAV
                     GYMDPGNWATDIEGGARFGYTLLSVILISNIFAIFLQYLSLKLGIVTNRDLAQACKDH
                     YSKPTAFILWILAEIAIAATDLAEVIGSAIAIHLLFGIPITIGIIITVIDVFILLLLQ
                     NRGFRYIEAFVGGLIFIISFCFVYNLLLAQPEIGAVLNGLIPKVDIIQNADMLYIGIG
                     ILGATVMPHNLYLHSSIVQTRAYGKDNISKKEAIKYATLDSTISLLLAFFINAAILIL
                     AAATFHKNELYEVTQIEDAYKMLEPLLGASLAPTFFAIALLAAGQNSTLTGTLSGQIV
                     MEGFINLKIKPWARRLITRLIAIIPALIVSIWMGERGTDRLLIFSQVILSLQLSFAVI
                     PLVLFTSDKDKMGEFANSKILTTFVWSIALIIISFNLYMLSGFIR"
     gene            complement(5213..6346)
                     /gene="hisB"
                     /locus_tag="PLS16_06825"
     CDS             complement(5213..6346)
                     /gene="hisB"
                     /locus_tag="PLS16_06825"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HNJ60830.1"
                     /translation="MKQKVLFIDRDGTIIVEPKDNFQTDKFEKLAFLPNVISALKRIV
                     NESNFKLVMVTNQDGLGTKSFPEKSFWGPHNLMLSILETEGVQFEDIYIDKTFAKDNA
                     PTRKPNTGLLTKYLDTSKYDLENSLVIGDRLTDIQLAKNIGAKGILIGRSADTTDDDK
                     LNQKALKPFIALKTNEWNKIADFICKPNRTASIQRNTNETQIQINLNLDGNGKSKIHT
                     GLGFFDHMLEQLSRHSGCDLEIKVKGDLHIDEHHTIEDTAIALGEAFYQAIGDKRGIN
                     RYGFLLPMDDVLAQVAIDFSGRSWLVWKAKFSREKVGDMPTEMFYHFWKSFSDAAKCN
                     LNIKAEGDNEHHKIEAIFKAVAKSIKMAIKKDGNELPTTKGLL"
     gene            complement(6381..9155)
                     /gene="uvrA"
                     /locus_tag="PLS16_06830"
     CDS             complement(6381..9155)
                     /gene="uvrA"
                     /locus_tag="PLS16_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNJ60831.1"
                     /translation="MPKVLTKNLEAQHIIVKGARMHNLKNIDVTIPRNNLVVITGVSG
                     SGKSSLTIDTIFAEGQRRYVESLSSYARQFLSRMDKPDVDYIKGLSPAIAIEQKTTTG
                     TTRSTVGTLTEIYDYLRLLYAKIGRTYSPISGNEVVKNEVADVLDFIKTLDDTQKIQI
                     LAPIHDTTTKGIQSLIAENIQRLLVDENAVIIDELDISKLPNANSVFALIDRAVAEKT
                     EENLHRLADSIQLAMSISNGTCVIDIPQKEKVSFSTAFELDGMEFEVPSVALFNFNSP
                     YGACKTCEGFGTVLGIDSDIVIPDKTISFYEGAVVPWRTTNGEYWKKQFIKGAAIVDF
                     PIHRPIMDLSKEQLNFLWKGGPGFLGINDFFKDVESQTYKIQYRVLLSKYRGKTDCPE
                     CKGSRLRADTEYVKINQKSIGDVLQMSIKDLKSFFDNIVLTEKEQHIAKRILLELRNR
                     LQFMSDVGLEYLTLNRFSNTLSGGEAQRINLTRNLGSNLTDSLYILDEPSVGLHPDDT
                     GKLIAILKHLKNLGNTVIVVEHEEEMMRQADFIVDVGPNAGYLGGEIVFVGTYNEILN
                     NDKSWTAKYLNHIIEIELPKQRRKPNYFFKIEGIFYHNIKNETVEIPLNSLTVVTGVS
                     GSGKTTLIKDIVYPILKTEVEKIPTKAGFYKNISGHYKTLTHIEFIDQNPIGRSSRSN
                     PITYIKAYDAIRDLFSKQSLSKIRGYQIRHFSFNIDGGRCDECKGEGEKVVEMQFLAD
                     VHLKCESCNGKRFKNEILEVQYQGKNIAEILELTVDEAIIFFEKEKDIASKLQPLQDV
                     GLGYIRLGQSSSTLSGGEAQRVKLASFLGKGNSKEKVLFIFDEPTTGLHFHDINKLLD
                     TFNALIEIGHSIIVIEHNLDVIKCADWLIDLGPKGGDEGGTILFQGVPENILNVKESI
                     TAKYLKDKLK"
     gene            9310..9888
                     /locus_tag="PLS16_06835"
     CDS             9310..9888
                     /locus_tag="PLS16_06835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNJ60832.1"
                     /translation="MREEFLSDEVLIHQYTLGNNKSIGTLFYRHQKKVVTYLYFKVND
                     QQLAEDIFIESFFRIIKSIDNKKYMENGKFYNLLIRVAKNSSIDYFRKIKSQPRIVDI
                     DGLVSIKNLRDNYEEQEYIEQNERVLEKIKAIILELPDDEREIVVLKHYADLTFREIS
                     ELTGINLNTALSKMRIALQRIRKMAEQRQIKY"
     gene            complement(9891..10217)
                     /locus_tag="PLS16_06840"
     CDS             complement(9891..10217)
                     /locus_tag="PLS16_06840"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNJ60833.1"
                     /translation="MNDFYLKVFEVVQQIPYGKVCTYGDIAEFLGTKSSSRLVGYAMN
                     YAHNYDTQIPAHRVVNRNGMLTGKFHFDSPTMMQQLLENEGIIVIDDKIQDFENKRWF
                     PNCLLD"
     gene            complement(10283..11146)
                     /locus_tag="PLS16_06845"
     CDS             complement(10283..11146)
                     /locus_tag="PLS16_06845"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNJ60834.1"
                     /translation="MTVIAHKLKVRNVHFNFDKVEKHWILGSAVATHFINSMHIVFPE
                     GEKFFIRSVRRFSKQVKSDELRTEINSFCGQEGVHSREHQRFWEVMEEQGLKPNLMAD
                     FLKLTAFSGKYSYENVLRNSLNKLQPQLGDKMALSVTTALEHYTAILANALFLEPMAT
                     NKNIAPQMLELLHWHAAEEIEHKAVCFDVLKEVDDSYILRISGMAHASLLLWGYLFAG
                     QMYFIYQDKDKSIIRMPIEMAVLMKTIVFGEIGKNLSKHLLLYFKKDFHPNDINDRHF
                     LENFFKDKSYA"
     gene            11379..12647
                     /gene="icd"
                     /locus_tag="PLS16_06850"
     CDS             11379..12647
                     /gene="icd"
                     /locus_tag="PLS16_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNJ60835.1"
                     /translation="MTPEKITIKDGKLVVPNNPIIPYIEGDGTGPDIWKASVKVFDAA
                     VEKAYNGTRKINWIEVLSGEKAFKQTGEWLPNDTLTTIQEHLIAIKGPLTTPVGGGIR
                     SLNVALRQILDLYACVRPVRYFNGVPSNSKVPDQVDMVIFRENTEDIYAGIEWLAGTP
                     EANKIIQFLKDEMNVTTKIRFPESSSIGIKPVSKEGTERLVRSALEYAVKHNRKSVTL
                     VHKGNIMKFTEGKFKEWGYQLAEKEFGDKVFTWVQYDKIAVEDGTSEANEAMKQAIAE
                     GKIIIKDAIADNFLQQIILRPDEYDVVATLNLNGDYISDALAALVGGIGIAPGANINY
                     VTGMAIFEATHGTAPKYANQDKVNPSSLILSGVMMLEFMGWTEAANLIVKGMEQSIEN
                     KTVTYDFHRLMDNATLLKCSEFGDAIIKSF"
     gene            complement(12714..13646)
                     /gene="mdh"
                     /locus_tag="PLS16_06855"
     CDS             complement(12714..13646)
                     /gene="mdh"
                     /locus_tag="PLS16_06855"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNJ60836.1"
                     /translation="MKVTVVGAGAVGATCADNIARKELCTELVMLDIRDGFTDGKAMD
                     MMQTAHLLGFDTKITGSTNDYSKTANSDVVVVTSGLPRKPGMTREELIGTNAGIVKGV
                     AENILKYSPNAIFIIISNPMDTMTYLTLKSTGIPKNRIIGMGGALDSSRFKYYLSQAL
                     NCSASDLNAAVIGGHGDTTMIPLTRLANLNGVPVTSLLDKETLDKVAADTMVGGATLT
                     KLIGTSAWYAPGAAGASLVEAVLRDEKKVVTCSVALEGEYGQSDICMGVPVVIGKNGW
                     EKILDYQLNEEEQAAFNKSADAVRAMNDVLKTVI"
     gene            complement(13777..14379)
                     /gene="gldD"
                     /locus_tag="PLS16_06860"
     CDS             complement(13777..14379)
                     /gene="gldD"
                     /locus_tag="PLS16_06860"
                     /inference="COORDINATES: protein motif:HMM:TIGR03512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HNJ60837.1"
                     /translation="MFTNKHLGFFCFLILFFAISCKTNYTPKRSGYFRLDFPKERNFV
                     TYNSASCPFIFEYADFGQIEYDSNDLKEEHEHPCWFNINYKDYNAKIYLSYTEISSSN
                     TLDNLIKDSYKLTFKHTIRADYIDETIVKGKHPNVSGMIYDVGGNAASGVQFYITDST
                     KNFLRGSLYFNVAPNIDSIAPAIQYFRNDVVHIINSLSWK"
     gene            complement(14385..>14891)
                     /locus_tag="PLS16_06865"
     CDS             complement(14385..>14891)
                     /locus_tag="PLS16_06865"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HNJ60838.1"
                     /translation="AKDLLLYLEETDSFDWQKLIKPAFFVPDSKKIDDLLEDFQNKRI
                     HMAVIVDEYGGTLGIVTLEDVLEEVIGEIKDEFDDVAEIDFKKIDNNNYIFEARTAIN
                     DIYKVLDIKDDVFDEIRGDADSLAGLMLEINHDLPKEGDELTYKNYNFIILEANDTRI
                     IRVKIVIK"
BASE COUNT         5067 a         2546 c         1985 g         5293 t
ORIGIN      
        1 catcgtaatc atcatcatag tcatcatcat cattacctcg aatattaata ttgacactat
       61 tcgagctatc acttttaata tctattttca cttgattatt tgcattgact tttctataag
      121 tttctttgtc ccaattgatt ggcatatcag aaatatcaat gtattcctcc gtttgtggtg
      181 tccattgaat cactttgttt tcaggaacat aaactctaac aatcaatttt tgatttctaa
      241 ttttacgttc tttagtatta aaactaaata gtgggtcaat gattagttta ttattcattt
      301 gcgtcagttg atgcgaaatt gaagtagcat tctttaaggc ttctttttct gttttaccaa
      361 aagatgaaaa ctctttctcc acataaaatg aatctaaagg cgttgtaaaa atttgaatat
      421 cgacatcatt ttcaatttca aaatctttac ctaaaaaact cattaaatcg ccaaaactat
      481 taatatttat ctgatgttct cttttagtca tcggtgaaat atctaatttt aaagtatcac
      541 ccattgcact gtaatttaat ggaacagact gcactatttt ttctttatgt ttgaaaaaag
      601 ctgctccttc ttttgttaca aatgagagga taaacagccc cgaaataatc aatccaacaa
      661 ttggcaatat ggtttttaaa ttaaatactt tatagttttt agaaaacaaa tgaaccaaca
      721 ataatatcaa tccaactaat gcagaaacgc cgaccaataa tcctcctaaa atcaataaaa
      781 ttgggctgtt ggtattggca tcaaataaaa actgttttgc aattggcgta ccaataaaaa
      841 cactaacaat aaaagctatt actgtaccaa ttaaagacaa aatgatgcta ccgaaaatga
      901 tgtataataa tactttactt aatttaatta aaatattaaa aaggatactt acaattttac
      961 caatagcatt tacgatatta tttaaagaac tagagctagt taattcttct atcttctcat
     1021 tttttttttc tacgatatta gataaattaa taggctctcc cttcatggct aatttttctg
     1081 ctgttgtttt agcttcaggc ataattatcc aaagaataat ataaaccaat aatgctgtac
     1141 caacagtaat aaatggcgaa ataatgaaaa ttattcttaa ccaaattggg tcatttatac
     1201 cgaaataggc tgtcaaacca ctacaaacac cagcgacaac actattgtct ttatctctgt
     1261 ataatctctt ttcagttggt gtactttttt gaaaatttgg ttttgtattt gttgtagttt
     1321 ccgcttgctc accatcttca aagtcttctg gtgttcccat cacagcaatc acttgattaa
     1381 catcttcaac tgccacaatt ctatcttttc ctttttggat aaacagttct gcaaagcgag
     1441 tttctatatc ttgcaaaatt tcttctttac cttcttcatt tgcatagata cgtttaatat
     1501 tattgaggta attatccaaa acatggtagg catcttcgtt aataacaaat gcttgtcctc
     1561 ctaaatttat attgaatgtc tttaacatat tgattaattt aattttttga atattggtta
     1621 ttttttagtt attgttcttt ctactgcatt agctagttct agccaagtaa attgcaattc
     1681 atctaatgct ccacgtcctt gctccgttaa tacataatat tttcttggcg gaccagaatt
     1741 gctttctttc cattcataag ccaataatcc taaatttttc aatcttgtaa gcaaaggata
     1801 aacggttccc tccaccacca acatatttgc agatttaagt tcatctaaaa tatcggaggc
     1861 ataaacctca cctcttgaaa taattccaag aatacagaac tccaaaatac ctttccgcat
     1921 ctgtacttta gtattctcta tactaaattg attttcaact gctgccattt attctatttt
     1981 aatttacaat aaagtattat tttgattgat ttccaaataa accctttttt aatcgtctat
     2041 cctaagaaaa tcgccatttg ttgaaaattt aagttctata tcattactta actcaacaga
     2101 gattccatta ttttctaaat tatattcaac tattgtgttg ccagaataat ttttatttac
     2161 ataatcacga acttttttag gtataatact ttctggtaat ttagattgtc cactaatatt
     2221 tttaatactt ttttcttttc catcaaattc taccttaatt ccattgctaa gaaaaacttc
     2281 atattcttta tacaaaatat cattgtccgt ttccactttc aacactttat aattaggaaa
     2341 atgagtattg atataagttt taacagagct tggagtttct gaataaggga taactctatc
     2401 tccagctgat acgataaaat ttgctgataa taatactgca accattgtgc cttttaaaat
     2461 acttgtagtt ttcatttttt ctttttttta gtttgattaa taatttagtt taattttact
     2521 tttactgtaa acacttgcat tacgccactt aggttggtaa attcaaatgc ttcaattgtg
     2581 tctataaatt tcaataactt tctaggcgaa acattattct ctatataagt tactgtcgga
     2641 tttccttttt catctacaac atattgaaca gtggtttctt tgtttttgtt tgcttcatat
     2701 ttgttaaatg caaaccaagc agaataggat aacaaactta tcatcataac tgccattccg
     2761 aatgtgagta cttttttcat tttttttatt tttttaaatt atttataata tcaagaactt
     2821 tacaatgcaa atataatgct tttacaatga acattgcaat acatagtact atatatctat
     2881 gttaaaaaat gttaattatc aatatttgta gggcttttag ctacaaatta atttattaat
     2941 ttttgaaata agagataaaa taaaactaat tattaccttt agcgtataaa ttaatttatg
     3001 aaaaagacat caattcttat ttattttacg attagtataa ttattttttc agcttgcaaa
     3061 acaagcaaaa ttacaacaac tcagaataca cttagtgaac aaagaaagct gaatgatata
     3121 gcattaaatg ggaaagtata tgcctcaata tggcaacaaa atgctggaga atttcgtgct
     3181 ttatgctatc aggcatttaa tattgctaaa aataatatag accaaaaaat cagctctaat
     3241 ccaacaagac cattggctat tattactgat attgatgaga cttttttaga taattctcct
     3301 tatgctgtaa ctcaagcatt acaaggcaaa gaattcagtt attcaacttg gctagaatgg
     3361 acttctttag gaaaagctat agcttatcca ggttctttag acttctttaa ttatgcagca
     3421 tcaaaaaatg taactgtatt ttatattaca aatcgtaatc aaaatgacaa acccggaaca
     3481 ctcaaaaact tacaagattt acattttcct tttgcagata atgaacatct aattgtaatg
     3541 gataagactt ctaataaaga acaacgaaga caagctattc aaaaaaacta tgatgttttt
     3601 atgtatcttg gtgataattt aggcgatttc tcagatgtat tttataagaa aacacaaact
     3661 gaacgcaata aaaataccga tgatttagca gatgaatttg gtaataaatt tatagtgctt
     3721 ccaaattcag gatatggcga ctgggaaaca gccttacctg gattcaattt cgatttaaca
     3781 ccagcacaaa aagactctgt cttcctaaag aatttaatag gttattagta aaatcattaa
     3841 cattttaatt ttcataaagc attaaattta tttacttttg taaaaaaaat ctgtgtctaa
     3901 gtggaaattt gaacgaacta acaatagctt acctgaagtt tattcaagta tttctgtaac
     3961 gcaaggaaaa aataaatgga ataaattctt agcgttttta ggacctggct taatggtggc
     4021 agttggctat atggacccag gaaattgggc tacagatatt gaaggtggtg caagatttgg
     4081 atatacctta ttaagtgtca tattaatctc caatattttt gccatttttt tacaatacct
     4141 atctttaaaa ttaggaattg taacaaatag agatttagca caagcttgta aagatcatta
     4201 tagcaaacca acggcattta tactttggat tttggctgaa attgctattg cagcaacaga
     4261 cttagctgaa gtaataggtt ctgcaattgc aatacatctt ttatttggaa ttccaataac
     4321 gattggaatt attattacag ttatagatgt ttttattctt ttattattac aaaaccgtgg
     4381 tttcaggtat attgaggctt ttgttggtgg attaattttt atcatttcct tctgttttgt
     4441 gtataattta ttactagctc aaccagaaat tggtgctgtt ttgaatggct taattccaaa
     4501 ggttgatatc atacaaaatg cagatatgtt gtatattgga ataggcattt tgggtgctac
     4561 ggtaatgcct cataatctat atttacattc tagtattgtt caaacaagag cttatggcaa
     4621 agataatata tccaaaaaag aagccattaa atatgctact ttagattcaa ctatttcatt
     4681 actattagca ttttttatca acgccgctat actcatttta gcagccgcta ctttccataa
     4741 aaatgaatta tatgaagtaa cacaaataga agatgcctat aaaatgttag aaccactttt
     4801 aggagcatca ttagcaccga cattttttgc tattgcactt ttagccgcag gacaaaactc
     4861 aaccctgaca ggtacattat cagggcaaat tgtaatggaa ggatttataa atttaaaaat
     4921 aaaaccttgg gctagaagat taattactag acttattgcc ataattccag cattaattgt
     4981 ttcaatatgg atgggagaaa gaggcactga cagattattg atatttagcc aagtaatctt
     5041 atcacttcaa cttagttttg cagtaattcc tttagtttta tttactagtg ataaagataa
     5101 aatgggagaa tttgccaatt ctaaaatatt aacaacattc gtgtggtcaa ttgcactaat
     5161 tattatttcc tttaacttat atatgctttc tggatttata agatagcaaa tattatagca
     5221 aacctttagt tgttggcaat tcgttaccat ctttcttaat tgccattttt atagactttg
     5281 caactgcttt aaaaatagct tcaattttat ggtgttcatt atcgccttcc gctttgatat
     5341 tcaaattaca tttagcagca tcactaaaac tcttccagaa atgataaaac atttcagttg
     5401 gcatatctcc tactttttct ctggaaaatt tagccttcca aaccaaccaa cttcttcctg
     5461 aaaaatcaat agcaacttgt gctaaaacat catccatggg caataaaaaa ccatatctat
     5521 taataccacg tttatcgcca attgcttgat aaaatgcttc gcctaatgca atagcagtat
     5581 cttcaatcgt atggtgttca tcaatatgca aatcaccttt cactttaatt tctaaatcac
     5641 aaccagaatg acgagataat tgttccaaca tgtggtcaaa aaaacctaat cctgtatgaa
     5701 tttttgattt cccattccca tctaaattca aatttatttg aatttgtgtt tcattcgtat
     5761 ttctttgaat tgaagctgtt ctgttaggtt tacagataaa atctgcaatt ttattccact
     5821 catttgtttt taaagcgata aatggtttta gtgctttctg atttaattta tcatcatctg
     5881 tcgtatctgc acttcttcca attaatatac cttttgcacc aatattttta gctaattgaa
     5941 tatcagttaa tctatcacca ataactaaag aattttctaa atcgtacttt gaagtatcta
     6001 aatattttgt caataaacct gtatttggtt ttcttgttgg agcattatct tttgcaaaag
     6061 ttttatcaat ataaatatct tcaaattgta caccttcagt ttctaaaata gacagcatca
     6121 aattatgtgg tccccaaaaa gatttttcag gaaaggattt tgtacctaaa ccatcttggt
     6181 ttgtaaccat tactaattta aaattacttt cattgactat cctttttagt gccgaaataa
     6241 cattgggtaa aaatgctaat ttttcaaatt tatctgtttg gaaattatct ttgggctcta
     6301 caataattgt tccatctcta tctataaata aaactttttg tttcataatc aattttaata
     6361 gtaaactaca aagatagcca ttatttcagt ttatctttca aatacttggc ggtaatactt
     6421 tccttgacat tcaaaatatt ttcagggaca ccttgaaaca aaattgtacc gccttcgtca
     6481 ccacctttgg gtcctaaatc aatcaaccaa tctgcacatt ttattacatc taaattatgt
     6541 tcgataacta taatagaatg accaatttca attaatgcat taaaagtatc taataattta
     6601 ttaatgtcat gaaagtgcaa tccagttgtc ggttcatcaa aaataaacaa gaccttttcc
     6661 tttgaatttc ctttgcctaa aaatgaagcc aatttcacac gttgtgcttc cccacctgaa
     6721 agtgttgagg aactttgccc taaacgaata taacccaatc caacatcttg caatggttgc
     6781 agtttagatg ctatatcttt ttctttttca aaaaaaataa tggcttcatc aactgtaagt
     6841 tctaatattt ctgcaatatt ttttccctga tactgtactt ctaaaatttc attcttaaat
     6901 cttttgccat tacaactttc acattttaaa tgaacatctg ccaagaattg catttcaacc
     6961 actttttctc cttcaccttt acattcatca caacgtccac catcaatatt aaatgagaaa
     7021 tgtcttattt gataacctct aatttttgat aaagattgtt tagaaaataa atctctaatt
     7081 gcatcataag cctttatata ggtaattgga ttagaccgag aacttctacc aataggattt
     7141 tggtcaataa actcaatatg tgtaagtgtt ttataatgac cacttatatt tttataaaaa
     7201 cctgctttag ttgggatttt ttctacttct gttttaagaa taggatacac aatatcttta
     7261 attaatgtag tttttccaga gccgctaaca ccagtaacta ctgtcaaact attcaaagga
     7321 atttcaacag tttcattttt gatattatga taaaagatac cttcaatttt aaaaaaatag
     7381 ttgggtttac gtctttgttt tggtaactcg atttctataa tatgatttag atattttgct
     7441 gtccaacttt tgtcgttatt cagaatttca ttataagttc caacaaaaac tatttctccg
     7501 cccaaataac cagcatttgg tccaacatca acaataaaat cagcttgcct catcatttct
     7561 tcttcgtgtt ccacaacaat caccgtattg cctaagtttt ttagatgctt taaaatagca
     7621 atcaattttc cagtatcatc tggatgcaaa ccaacacttg gttcatctaa aatatataaa
     7681 gaatctgtta ggttacttcc caaatttcta gttagattaa tccgctgtgc ttcccctccc
     7741 gataaagtat tgctaaatct attcagtgtc aaatattcta acccaacatc gctcataaat
     7801 tgtaaacgat tacgcaattc tagcaaaatt ctttttgcaa tatgctgttc tttctctgta
     7861 agtactatat tatcaaaaaa actttttaaa tctttaatag acatttgcaa tacatctcca
     7921 atactttttt gattaatttt gacatattcc gtatctgctc gaagtcttga acctttacat
     7981 tctggacaat ctgtttttcc tctatatttt gataataaaa ctcgatattg aattttatag
     8041 gtttgtgatt caacatcttt aaaaaaatca ttaatgccta aaaatcctgg accacctttc
     8101 cataaaaaat ttaactgttc tttcgacaaa tccattatcg gtctatggat aggaaaatca
     8161 acgattgcag ctccttttat aaattgtttt ttccaatatt ctccatttgt agtacgccat
     8221 ggaacaactg caccttcata aaacgaaatt gttttatctg gaattacaat atcactatca
     8281 attcctaaaa ctgtaccaaa accttcgcat gttttacaag ctccataagg cgaattaaaa
     8341 ttaaataagg caaccgacgg cacttcaaac tccattccgt caagctcaaa agctgttgaa
     8401 aagcttactt tttctttttg tggaatatca ataacacaag tcccattact aatgctcatt
     8461 gccaattgaa tagagtccgc taaacgatgc aagttttctt cagttttttc tgcaactgct
     8521 ctgtcaatta aagcaaaaac tgagtttgcg tttggtaatt ttgaaatatc taattcatca
     8581 ataatgactg cattttcatc tactaataat ctttgaatat tttcagctat taaagattga
     8641 ataccttttg tagtagtatc atgtattggt gctagaattt gtatcttttg tgtatcatct
     8701 aaggttttta taaaatccaa aacatcagca acttcattct ttacgacttc gtttccagaa
     8761 attggcgaat aagttcttcc aatcttagca tacaataatc tgaggtagtc ataaatttct
     8821 gtcaatgtgc caactgtact acgtgttgtt cctgttgttg tcttctgctc aatagcaatg
     8881 gctggtgata gtccttttat ataatccaca tcaggcttat ccattctgct taaaaactgt
     8941 cttgcataag atgatagtga ttctacataa cgcctttgtc cttcagcaaa aattgtatca
     9001 atcgtcaatg atgattttcc acttccactc acacctgtaa taaccaccaa attatttctt
     9061 ggtatcgtta catcaatatt tttaagattg tgcattcttg cacctttcac aataatatgt
     9121 tgagcttcta aatttttcgt taaaacttta ggcattacag cgaatttaag actttattag
     9181 gttaatcaat gttaaggaat gtacaagatt aaaatcattt acataaaatt gtcttaataa
     9241 taatttcaca caaaatgata gacttctaca tgattcattt tattaacgca tcaacaaaaa
     9301 taaaagttta tgagagaaga atttttaagc gatgaagtac tcatacatca atatacttta
     9361 ggcaacaata aaagcatagg tacattattt tatagacatc aaaaaaaggt cgttacctat
     9421 ttatatttta aagtgaatga ccaacagctt gctgaagata tttttattga atcatttttt
     9481 agaatcatca agtccataga taataaaaaa tatatggaaa atggtaagtt ttataattta
     9541 cttatccgag tagccaaaaa ttcgagcatc gattatttta gaaaaataaa aagccaaccc
     9601 cgaattgtcg atattgatgg attagtatcc ataaaaaatt taagagataa ttacgaagag
     9661 caagaataca tagaacagaa tgaaagagta ttggaaaaga taaaagcaat cattttagaa
     9721 ttgcctgatg atgaacgtga aattgtcgta ctcaaacatt atgcagattt gacttttaga
     9781 gaaatttcag aattaactgg tataaatctc aacacggcat taagtaaaat gcgaatagct
     9841 ttacaacgaa tccgaaaaat ggcagaacaa cgacaaatta aatattaagt tcaatctaat
     9901 aaacaattag gaaaccatct tttattttca aaatcttgaa ttttatcatc gatgacaata
     9961 attccttcgt tttctaagag ttgttgcatc attgttggtg aatcaaagtg aaactttcca
    10021 gttagcatac catttctatt gaccactcta tgtgcaggaa tttgtgtgtc ataattatga
    10081 gcgtaattca tagcataacc tactaatctt gacgaagatt ttgttcctaa aaattcagca
    10141 atatcaccat aagtacaaac tttgccataa ggaatttgtt gtaccacctc aaatactttc
    10201 agataaaaat cattcataga taatagaaaa aaatagttcc tctaaaatag gaactatttt
    10261 taataatctc taaaatgtta aatcaagcgt aacttttatc tttaaagaaa ttctctaaaa
    10321 aatgtctgtc attaatatca ttagggtgaa aatctttttt gaaatacaat aataaatgct
    10381 tagaaagatt tttgccaatt tctccaaaga caattgtttt catgagtaca gccatctcaa
    10441 ttggcattct gattatactt ttatctttat cttgataaat aaaatacatt tgacctgcaa
    10501 ataaataacc ccaaagcaac aaagaagcat gtgccatacc tgaaattcta agaatataac
    10561 tatcatcaac ttccttcagc acatcaaaac aaactgcttt gtgttcaatt tcttcagcgg
    10621 catgccagtg caataactcc aacatttgtg gtgcaatgtt tttatttgta gccataggct
    10681 ccaaaaataa ggcattggct aaaattgctg tataatgttc taatgctgtc gtaacagaaa
    10741 gtgccatctt atcgcccaat tgaggttgca atttattcag tgaatttctt aatacatttt
    10801 cataactata cttccccgaa aaagctgtaa gctttaaaaa atctgccatt aagtttggtt
    10861 ttagcccttg ctcttccata acttcccaaa atcgttgatg ctcacgagag tgtacacctt
    10921 cttgtccgca gaaactattg atttctgttc ttaattcatc actttttact tgtttagaaa
    10981 atcgtcgaac actgcgaata aaaaactttt cgccctcagg aaatacaata tgcatactat
    11041 tgataaaatg cgtggctact gcactcccta atatccaatg tttctcaact ttatcaaaat
    11101 taaaatgtac gtttctaact tttaatttgt gtgctattac agtcataact ttaaatttat
    11161 tatcataaaa ttagacctta aaacaactgc aacctattat ctttggataa atatacttta
    11221 aaagaattag aacttatctt ttgtttaaac atctacattt atacaaaaaa aaacaaaact
    11281 ttatttgcta tatattgtta atggagtgaa gagataaaat actttttatt atatttgaat
    11341 gcagaaacat ctaaatcaat tttataaaat atatcattat gacacctgaa aaaattacaa
    11401 ttaaagatgg gaaattagta gttccaaaca atccaataat tccatatatt gaaggcgatg
    11461 gaactggtcc tgacatctgg aaagcatctg taaaagtttt tgatgccgca gtagaaaaag
    11521 cctataacgg aacgagaaaa ataaattgga ttgaagtact ttctggggaa aaagctttta
    11581 aacagactgg cgaatggttg cctaacgata cactaacaac aatacaagaa catttaattg
    11641 caattaaagg acctttgact actccagttg gtggtggtat tcgttctttg aatgtggctc
    11701 ttagacaaat tttagattta tatgcatgtg tacgacctgt tcgttatttc aatggtgttc
    11761 cttccaattc aaaagttcct gaccaagtag atatggttat tttcagagaa aatactgaag
    11821 atatttatgc tggaatcgaa tggttggctg gcactccaga ggctaataaa atcattcaat
    11881 ttttgaaaga tgagatgaat gttactacaa aaattcgttt cccagaatca tcttctattg
    11941 gaataaaacc agtatctaaa gaaggaacag agcgtttagt acgttctgct ttagaatatg
    12001 ctgtaaaaca caacagaaaa tctgtaacct tggtacacaa gggaaatatt atgaaattta
    12061 ctgaaggtaa gtttaaagaa tggggttatc agttagctga aaaagaattt ggtgataaag
    12121 ttttcacttg ggttcaatat gataaaattg cagtagaaga tggaacttct gaagcaaatg
    12181 aagcgatgaa acaagccatt gccgaaggca aaatcattat taaagatgct attgctgaca
    12241 acttcttaca acaaattatt cttagaccag atgaatacga tgttgttgca acattgaact
    12301 taaatggcga ctacatttca gatgcattag ctgcattagt gggtggaatt ggtattgctc
    12361 caggtgcaaa catcaactat gtaacaggaa tggcaatctt tgaagcaaca cacggaactg
    12421 ctccaaaata tgctaaccaa gacaaagtta atccgagttc cttaattctg agcggtgtga
    12481 tgatgttaga atttatgggt tggactgaag ctgccaactt aatagttaaa ggtatggaac
    12541 aatctataga aaataaaacg gtaacttacg attttcatag attgatggat aatgcaactt
    12601 tattaaaatg ctctgaattt ggagatgcaa ttataaaaag tttttagtaa ggttcagata
    12661 ctaaataaaa aaggctgcct atagcagcct ttttctattc tataaaacaa taattagatt
    12721 actgttttta aaacatcatt catcgctcta acagcgtcag cacttttatt aaaagcagct
    12781 tgttcttctt cgtttaattg ataatctaat attttttccc atccattttt accaattaca
    12841 acaggaacac ccatacaaat atcagattgt ccgtattcgc cttctaaagc aacactacaa
    12901 gtaacaactt ttttctcatc tctcaacact gcttcaacaa gagaagctcc agcagcaccc
    12961 ggtgcatacc aagcagaagt cccaattaac ttagttaaag tagcaccacc aaccattgta
    13021 tcagcagcaa ctttatctaa agtttctttg tctaataaag atgtaactgg aacaccattt
    13081 aagttagcta atcttgtaag tggaatcatt gttgtatcgc catgaccacc aataacagca
    13141 gcattcaaat cagaagctga acagtttaaa gcttgagata aatagtattt aaaacggcta
    13201 ctatctaatg ctccacccat tccaataatt ctatttttag gaatacccgt agatttcaat
    13261 gtcaaataag tcatagtatc cattggatta gagataataa taaaaatagc atttggagaa
    13321 tattttaaaa tattttctgc cacacctttt acaatacctg cgttagttcc gattaattct
    13381 tcacgagtca ttcctggttt tctaggaagt cctgaagtta ctacaacaac atcactgttg
    13441 gcagttttag aataatcatt ggtagaaccc gtgatttttg tatcaaatcc taataaatgt
    13501 gctgtttgca tcatatccat agctttacca tctgtaaaac catctctgat gtctaacatt
    13561 actaattctg tgcacaattc tttacgagca atattatctg cacaagttgc accaactgcg
    13621 cctgctccta ctactgttac tttcattttg tattattttt ttatgtaaac tgaaaaatta
    13681 aatttcaata caaatataca aaaacattga tgttcaacaa gaatatttca ttcattttag
    13741 attaactata agttatgctg atgtttcaaa gaaacattat ttccaactca atgagttgat
    13801 aatatgcaca acatcatttc taaaatactg tattgctgga gcgatagagt cgatatttgg
    13861 agctacatta aaatacaatg aaccacgaag gaaattctta gtactgtctg ttatataaaa
    13921 ttgaactcca gatgctgcat ttccacctac atcataaatc atacctgata catttgggtg
    13981 ctttcctttg actattgttt catcaatgta gtctgctcgt atagtatgtt tgaaagtaag
    14041 cttgtaacta tctttaatta aattatctaa tgtattggaa ctagaaattt cagtataact
    14101 tagatatatt ttagcattat aatctttata atttatatta aaccaacaag gatgttcatg
    14161 ttcttccttc aaatcattac tatcatattc aatctgtcca aaatcagcat attcaaatat
    14221 aaaaggacaa ctcgctgaat tgtaagttac aaaattccgt tcttttggaa aatctaatct
    14281 aaaataacct gaacgtttgg gcgtataatt tgttttgcaa gaaattgcaa aaaacaaaat
    14341 caaaaaacaa aaaaatccta aatgtttatt tgtaaacata atttctattt gataacgatt
    14401 ttaacacgaa taattcttgt atcattcgcc tccaagataa taaaattata attcttataa
    14461 gttaattcat caccttcttt cggtaaatcg tggttaattt ccaacatcaa tccagctaat
    14521 gaatccgcat ctcctcttat ttcatcaaaa acatcatctt tgatatccaa aactttataa
    14581 atatcattaa ttgcagttct tgcttcgaaa atgtaattgt tattgtcaat ttttttaaaa
    14641 tcaatttctg caacatcatc aaactcgtct ttaatttctc caatcacttc ttccaaaaca
    14701 tcttctaaag taacaattcc aagtgtacct ccgtattcat caactatgac tgccatgtga
    14761 attcttttgt tttgaaaatc ttctaataag tcatctattt ttttgctatc aggaacaaaa
    14821 aatgcaggtt ttatcaactt ttgccaatca aaactatctg tttcttccaa atatagtaat
    14881 aaatctttcg c
//
LOCUS       DAOQCW010000062        14833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1483_length_14833_cov_5.965828, whole genome shotgun sequence.
ACCESSION   DAOQCW010000062 DAOQCW010000000
VERSION     DAOQCW010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14833
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1483_length_14833_cov_5.965828"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PLS16_06870"
     CDS             complement(<1..233)
                     /locus_tag="PLS16_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNJ60839.1"
                     /translation="MNFPIIAPSVLSANFANLEADFKMLNESEAEFIHIDVMDGVFVP
                     NISFGLPVIQAMKKLSNKTFDVHLMIVEPQNYI"
     gene            complement(253..810)
                     /locus_tag="PLS16_06875"
     CDS             complement(253..810)
                     /locus_tag="PLS16_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNJ60840.1"
                     /translation="MQSEKLLQSLIEQTNQITYQAEKFKTFDLDILTWRENNTSWNIL
                     ECLEHLNLYGDFYLLQIETKIKNSKTKSELDIKSGLLGNYFAESMLPKEKLNKMKTFK
                     DKNPLNAQLDKSVIDKFLSQQKKLISILHQAKNVSLNKIKIETSISSLLKLKLGDTFQ
                     FFINHIVRHFKQIEKIQIAMYSNKY"
     gene            complement(866..1372)
                     /locus_tag="PLS16_06880"
     CDS             complement(866..1372)
                     /locus_tag="PLS16_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNJ60841.1"
                     /translation="MKEIIEFSDKTVTIDRNEFLKLSGSIDTNIYFVESGSLRIFISD
                     DVGEQIIRFAYKDNIFVALDSFLTGKSSGFSIQAIKKTVVKVISKQQFIRFLEIEKNR
                     NLWTKILENLVLQQMEREIDLLINSPKERYERVLQRSPQLFQEIPNRYIANYLRMSAE
                     TLSRLKKS"
     gene            complement(1422..1718)
                     /locus_tag="PLS16_06885"
     CDS             complement(1422..1718)
                     /locus_tag="PLS16_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60842.1"
                     /translation="MGQLFKYVFLSLIAIALLQVIFTKEEKKDTYTPAVRDTTRKTIG
                     YDWQRGVYVYEDEGLAKNSEGYWVPENEIVKPKELKLDPKDKDEPLSAFDLPNY"
     gene            complement(1786..2190)
                     /locus_tag="PLS16_06890"
     CDS             complement(1786..2190)
                     /locus_tag="PLS16_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HNJ60843.1"
                     /translation="MKYWLMKSEPDVFSFDDLIAKGQEHWDGVRNYTARNNMQAMKVG
                     DKAFFYHSNIGKEIVGIVKIVKEAYQDPTTDETRWVCVDVAPIKKLKNPVSLAAIKAN
                     PKLAEMKIIKQSRLSVIDVTKAEFDEILEMSK"
     gene            2355..5126
                     /locus_tag="PLS16_06895"
     CDS             2355..5126
                     /locus_tag="PLS16_06895"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HNJ60844.1"
                     /translation="MSIYQDYLQEIEERKKQGLHAKPIDEAPLLTAIIEQIKDLGNPH
                     RADSLNFFIYNTLPGTTPAAAVKALFLKDIILGNTVVAEITPDFAFELLAHMKGGKSI
                     EVLLDIALGNDTTTAKKAAEVLKTQVFLYDADTARLKEAYENGNAIAKEILESYAKAE
                     FFTKLPEVPEAIKVVTYIAAEGDISTDLLSPGNQAHSRSDRELHGKCMNSPEAQAEIR
                     ALQAKFPDASVMMIAEKGTMGVGSSRMSGVNNVALWTGKQSSPYIPFVNIAPIVGGTN
                     GISPIFLTTVAVTGGIGLDLKNWVKKVDADGNVVKAENGDPVLEEVYSVATGTELTIN
                     TKTKKLYNGDKELIDISSALTPQKMEFIKAGGSYAVVFGKKLQTFAAETLGIQAPVVF
                     APSKEITIEGQGLTAVEKIFNKNAVGVTPGKVLHAGSDVRVKVNIVGSQDTTGLMTAQ
                     ELESMAATVISPTLDGAYQSGCHTASVWDKKAQANIPKLMSFMNNFGLITARDPEGVY
                     HSMTDVIHKVLNDITINDWAIIIGGDSHTRMSKGVAFGADSGTVALALATGEASMPIP
                     ESVKVTFKGQMKEYMDFRDVVHATQAQMLQQFGGENVFQGRVIEVHIGTLPADQAFTF
                     TDWTAEMKAKASVNISEDATLIQSLEIAKSRIQIMIDKGMDNDNQVLKGLIDKANNRI
                     AEIQSGVNPALRPDANAKYFAEVVVDLDQIVEPMIADPDVNNKDVSKRYTHDTIRPLS
                     YYEGERKVDLGFVGSCMVHKGDLKIVAQMLRNVEAEKGKVEFNAPLVVAAPTYNIIDE
                     LKAEGDWDILEKYSGFEFNDAAPKNTARTEYENIMYLERPGCNLCMGNQEKAAKGDTV
                     MATSTRLFQGRVVEDSATKKGESLLSSTPVVVLSAILGRTPNIEEYQSAVKGINLTKF
                     KPSSMQLTK"
     gene            complement(5199..5729)
                     /locus_tag="PLS16_06900"
     CDS             complement(5199..5729)
                     /locus_tag="PLS16_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60845.1"
                     /translation="MTNYNIGTKLEHPTFGKGVIIDNEDEYLYNIYFSEHGEKEISKA
                     FDGMLVIKLVEKDNSGISLDEVEKTIYQMIKPLIEPIEDCKIADRYQGGKLIIQSADT
                     SLQDKEIDLESFFHKIVMTRDRLRVMEQKINASKLTDAEKVELQQYITRIYGSLTTFN
                     VLFKYKDDYFKGTGGA"
     gene            5966..6103
                     /locus_tag="PLS16_06905"
     CDS             5966..6103
                     /locus_tag="PLS16_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60846.1"
                     /translation="MRIKKSCLSFNPPSPLLVATNNGERNKELSQKLKTPFEGDGDNN
                     Y"
     gene            complement(6124..6399)
                     /locus_tag="PLS16_06910"
     CDS             complement(6124..6399)
                     /locus_tag="PLS16_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HNJ60847.1"
                     /translation="MSYEIEYSGKIKKDIKLAQKRGLDMELFKEAVIYLENDGKLPAK
                     YKAHILSGNWKGYWECHIQSDWLLIWKQDEVIKLVTLIRTGTHFDLF"
     gene            complement(6396..6611)
                     /locus_tag="PLS16_06915"
     CDS             complement(6396..6611)
                     /locus_tag="PLS16_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60848.1"
                     /translation="MEAIKLKINKRTKYGKALLELIKIGVSENKGIEIIKEKVPNEAT
                     IKAIQDVEKGKTFKVNNSKDLFLGLVI"
     gene            6895..10293
                     /locus_tag="PLS16_06920"
     CDS             6895..10293
                     /locus_tag="PLS16_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNJ60849.1"
                     /translation="MQNPTPYQPKNKIRIVTAASLFDGHDVAINIMRRIMQSSGAEVI
                     HLGHNRSVKEIVDCAIQEDANAIAITSYQGGHVRFLKYMYDLLQEQNSNQIKIVGGGG
                     GTILPSEIEELEQYGIDKIYSPDDGREMGLQGMINEVLEIADFATGKDISLDTELLKQ
                     KNTKQIAQLISCAENFPKEFEKTAAQISSLTAHNQTPILGITGTGGAGKSSLTDEIVR
                     RFLLDFPDKSVAIISIDPSRRKTGGALLGDRIRMNSINNERVFMRSMATRQTNASISK
                     DVQDAIQILKAAAYDLIIVETSGIGQSGTEIIDIADVFLYVMTPEFGAASQLEKIDML
                     DYADIVAINKFDKRGAQDALRDVKKQYQRNRNLWDKNPDEMPVFGSIAAQFNDPGTNE
                     LYLNLIQTIITKTKVDWRSQLNLKTGNSEKIYIIPPNRTRYLSEIVKTNKDYDVWVNE
                     QAEIANALYALDRVQSIVQSSQSNKNQESRTKNQELLPIDQLRNEQQQKLHHDCKQIL
                     DNWTEKVQSYKNKEFVFKVRDKEIKIKTHTTSLSHQEIPKVALPKYTAWGDILKWNLQ
                     ENVPGSFPYTAGVFPFKRENEDPTRMFAGEGGPERTNKRFHYVSLGLPAKRLSTAFDS
                     VTLYGNNPDLRPDIYGKIGNSGVSICTLDDAKKLYSGFDLCSPTTSVSMTINGPAPTI
                     CAFFMNAAIDQQCEKYIKENNLTTIVEQKIDAIYKAKNVTRPCYYGKLPEGNDGLGLM
                     LLGVTGDQVLEKDIYEKIKKYTLSQVRGTVQADILKEDQAQNTCIFSTEFSLKLMGDV
                     QSYFVENKVRNFYSVSISGYHIAEAGANPISQLAFTLANGFTFVEYYLSRGMHIDDFA
                     PNLSFFFSNGIDPEYAVIGRVARKIWAKAMKYLYKGDERSQKLKYHVQTSGRSLHAQE
                     ISFNDIRTTLQALYAIYDNCNSLHTNAYDEAITTPTEESVRRAMAIQLIINKELGLAK
                     NENPLQGSFIIEELTDLVEEAVYTEFERMTERGGVLGAMETMYQRGKIQEESMYYENL
                     KHTGNYPIIGVNTFLSSKGSPTVIPDEVIRSTDEEKNNQIDNLNALHQRNENDSIKLL
                     KTLQQTALENKNVFEVLMETVKYCSLGQITQALFEVGGQYRRNM"
     gene            complement(10353..11312)
                     /locus_tag="PLS16_06925"
     CDS             complement(10353..11312)
                     /locus_tag="PLS16_06925"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HNJ60850.1"
                     /translation="MKWIQTYTGFWRNLRLLHFIYNYINRQKLKRNKDLYKRFGINKS
                     VYSSISSKDFIGKPNRLPWLDYPVTPEKLVEHPVYMQFDKSVQSALLDWHKNGFLVLP
                     SFYKDTIIDKINQEIELLLETEMLQFNYTKRKVMNAFQYSEIIRQTIQDSRLKSILEF
                     ILENPVIPFQTINFIQGSEQAPHSDAIHMSTYPQGNLIAAWIALEDIQLEAGAIAYYP
                     GSHQLPYLTNKDIGAKNNFLFLDANANLKHEIKTAKLIKDNHLKPSILTAKKGDVLIW
                     HSNLIHRGLPIQNPKLTRKSMVTHYFTKDVICYHEISERPALI"
     gene            complement(11326..12741)
                     /gene="accC"
                     /locus_tag="PLS16_06930"
     CDS             complement(11326..12741)
                     /gene="accC"
                     /locus_tag="PLS16_06930"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNJ60851.1"
                     /translation="MKKILVANRGEIALRVMRTCKKMGIATVAVYSTADRKSPHVRFA
                     DEAVCIGEPASKQSYLDIDKIIKVCKQLNVDGVHPGYGFLSENAQFAKACEENGIVFI
                     GPSVLAIEVMGSKLAAKQAVGKYNVPLVPGTDQPITDIAEAKKLAAEIGFPILIKASA
                     GGGGKGMRVVENEAEFEEQMNLAVSEAVSAFGDGAVFIEKFVQSPRHIEIQVLGDAHG
                     NVVYLFERECSVQRRHQKVVEEAPSALLTPEVRKKMGEAAVNVAKAVNYVGAGTVEFI
                     ANNNLDFYFLEMNTRLQVEHPVTEMITGKDLVKEQIQIAEGKPLSFSQEDLKINGHSI
                     ELRVYAEDPNNNFLPDIGTLDTYQVPTGDGVRVDDGFDEGMEIPIYYDPMIAKLIAHA
                     ENRDAAIQKLLNAIDDYKIEGVKTTLGFGKFVLNHPAFISGDFDTKFVDNYFHPTNIN
                     QKELKMASIMAASLYAQFNKK"
     gene            12810..13442
                     /locus_tag="PLS16_06935"
     CDS             12810..13442
                     /locus_tag="PLS16_06935"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60852.1"
                     /translation="MLKKEFLLEWRSKASIGSLLVHALATIFFIYMIFNGDINFITWL
                     ALFWVINLFSVIQITARTFQKDANEHFYYLKQLASAQHIILSKIIYNTIFIIIISFIV
                     FLFMQLFFSQQVTYLGIFSLTILLASLGFSALFSLLSGISAKTQNMVLLAVLGLPIIL
                     PLILIISKLSALADFYTTINDIYIFLSAAVLLDITILILAIILFPYLWKD"
     gene            13455..14129
                     /gene="ccsA"
                     /locus_tag="PLS16_06940"
     CDS             13455..14129
                     /gene="ccsA"
                     /locus_tag="PLS16_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNJ60853.1"
                     /translation="MKQNWWKFVASIFLIYSLIAGFLLPIPELNILEQSIRNLFFHVP
                     MWFSMMAMMFISLWYSIKYLNSNKTIYDIKASAFAQVGFVLGLLGITTGSVWAKVTWG
                     AWWVFSEPKLNGAAAALLVYAAYFILRSSFDDEEKTARFSAVYNLFAFVMFMVFINIL
                     PRLTDSLHPGNGGNPGFNAYDLDSRLRAVFYPACIGWIAIAYWIGNIVVRIKQLNRKI
                     LMLEID"
     gene            14137..>14833
                     /locus_tag="PLS16_06945"
     CDS             14137..>14833
                     /locus_tag="PLS16_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60854.1"
                     /translation="MNLKPYLFFIFVFYFVFLNGAENGYKYWKKANQLFEQKKYQEAL
                     IYYDKIDKNIDILDVKINSARCLYFLEKFVEGEIAYQKIIETPNINLDVYKEYANILI
                     KNGKANEAKIWDKKYIEENIKQQKFITEIPKNPTKVDSSKTKVVSSKTDKVQKLKPIK
                     EDSSKTKVVSSKNQVVNNKIDKEQKLKPIKDTVKIEKPKAIKQKDTTAKTIKTPKVKP
                     TKVDSSKTKVVSNK"
BASE COUNT         5118 a         2332 c         2350 g         5033 t
ORIGIN      
        1 tgaatataat tttgaggttc tacaatcatt aaatgaacat caaaagtttt gttactcaat
       61 tttttcatag cttgaattac aggtaatcca aatgaaatat taggtacaaa aacaccgtcc
      121 attacatcaa tatgaataaa ttcagcctca ctttcgttca gcattttaaa atcagcttct
      181 aaattggcaa aatttgcaga taaaaccgat ggagctatta ttggaaaatt catgtacaaa
      241 gatacatttt tttcagtatt tattactgta cattgctatt tgtatttttt caatttgttt
      301 aaaatgtctg acaatatgat taataaaaaa ctgaaatgta tcgcccaact ttaatttcaa
      361 tagacttgat attgacgtct caatttttat cttatttaaa ctaacatttt tggcttgatg
      421 gagaatagaa attaactttt tttgctgact taaaaactta tcaattacgc ttttatcaag
      481 ttgtgcattc aaaggatttt tatctttaaa agtcttcatt ttatttagtt tttctttagg
      541 caacatactt tctgcaaagt aatttcccaa taatccactt ttaatatcga gttcagattt
      601 tgttttagaa tttttgattt tagtttcaat ctgtaacaaa taaaaatctc cgtataaatt
      661 tagatgctcc aaacattcca aaatattcca agacgtatta ttttctctcc aagtcaaaat
      721 atccaaatca aaggttttaa atttttcagc ttgatatgta atttgattag tttgctctat
      781 taatgattgt aataattttt ctgattgcat aattaaaatt ttatgcaaag ttttaaaatg
      841 tttttctgat aaacattgat tgtaatcaag acttttttaa tcgagataat gtttcggcac
      901 tcattctcaa ataattggca atatatctgt ttggtatttc ttgaaataat tgggggcttc
      961 tctgcaaaac cctttcgtat ctttcttttg gtgaatttat caaaagatca atttctcgtt
     1021 ccatttgttg aagcaccaag ttttctaaga tttttgtcca taaatttcta tttttctcaa
     1081 tttctaaaaa acgtataaat tgctgtttac ttataacttt tacaactgtt ttttttattg
     1141 cctgaataga aaatcccgaa gattttcctg ttaaaaaaga atctaatgca acaaatatat
     1201 tgtctttgta tgcaaaccta ataatttgtt ctccaacatc atcagagata aaaattctta
     1261 aacttccgct ttcaacaaaa taaatatttg tatcaatact gccactcaat tttagaaatt
     1321 catttctgtc tattgttact gttttatctg agaactcaat aatttctttc attgtttaaa
     1381 ctcactaaaa tataaaaaat gataatgaaa gtaaatgatt tttagtaatt aggcaaatcg
     1441 aatgcagaaa gtggttcgtc tttgtctttt ggatctaatt ttaattcttt tggttttaca
     1501 atttcatttt ctggcaccca ataaccttct gagttttttg ctaaaccttc atcttcataa
     1561 acataaacac cacgttgcca atcataacca attgtttttc ttgtagtgtc tctaacagca
     1621 ggtgtgtatg tatctttttt ttcttctttg gtaaaaataa cttgcaataa agcgatagca
     1681 attaaagata aaaatacata tttaaacaac tgtcccatga tataaatata tgttttttat
     1741 ttgatataat tcaatttaaa caaattatca aacctatcat caaaattact tactcatttc
     1801 caaaatttca tcaaattcgg ctttggttac atcaataacc gacaatctac tttgtttaat
     1861 aattttcatt tcagctaatt taggattggc ttttattgca gccaaactta caggattttt
     1921 aagttttttt attggagcaa catctacaca aacccatcta gtttcatcag ttgttggatc
     1981 ttgataagct tctttcacaa ttttcacaat acccacaatt tcctttccaa tattagaatg
     2041 ataaaaaaaa gccttatcgc ctactttcat tgcttgcata ttatttcttg ccgtataatt
     2101 tcgcacaccg tcccaatgtt cttgtccttt tgctatcaaa tcatcaaaac taaacacatc
     2161 aggttcagat ttcattaacc aatatttcat aatacttttt atttagaatt gatataaata
     2221 tagtgttgta aattaatcat ttttaaacaa taaagcattg aaaaatcatt aaaaattgag
     2281 tacatttgcc gtcgcaaata aattttatca aaatacagac gaatacgtct atcatattaa
     2341 aaaaaacttc tattatgagc atttaccaag attatcttca agagattgaa gaaagaaaaa
     2401 aacaaggact tcatgcaaaa ccaattgatg aagcaccatt attaactgca attatcgaac
     2461 aaattaaaga tttaggtaat cctcacagag cagattctct aaacttcttt atctacaata
     2521 ccttaccagg cacaacacct gcggcagctg ttaaagcact tttcttaaaa gatattattt
     2581 taggaaatac cgttgttgct gaaattacac ctgattttgc ttttgagtta ttggcacaca
     2641 tgaaaggtgg aaaatcaatt gaagtattat tggatattgc attaggaaat gatacgacaa
     2701 ctgctaaaaa agcggcagaa gtacttaaaa ctcaagtttt cctttatgat gcagatactg
     2761 ctcgtttgaa agaagcctac gaaaatggta atgccattgc taaagaaata ttagaaagct
     2821 atgctaaagc agaatttttt actaaattac cagaagttcc tgaagctatt aaagtagtaa
     2881 cttatatcgc agcggaaggt gatatctcta cagatttatt atctccagga aatcaagctc
     2941 actctcgttc tgatcgtgaa ttgcatggaa aatgtatgaa ctctccagag gcacaagcag
     3001 aaattagagc attacaagca aaattcccag atgcaagtgt gatgatgata gcagaaaaag
     3061 gtacgatggg tgttggctct tcaagaatgt ctggtgtaaa caacgtggca ctttggactg
     3121 gtaaacaatc tagtccttat attccatttg taaatattgc tcctattgtt ggcggaacaa
     3181 acggaatttc tccgattttc ttaaccacag ttgcagtaac tggaggaatt ggacttgacc
     3241 ttaaaaattg ggtaaagaaa gttgatgctg atggaaatgt agtaaaagct gaaaatggag
     3301 acccagtttt agaagaagta tattctgttg ctactggaac tgaattaacg attaatacaa
     3361 agactaagaa attatacaat ggagataaag aattaattga tatttcaagt gctttaacac
     3421 cacaaaaaat ggagtttatt aaagctggag gttcttatgc tgttgtattt ggtaaaaaat
     3481 tacaaacctt tgctgctgaa actttaggaa ttcaagctcc tgttgttttt gcaccttcaa
     3541 aagaaattac gattgaagga caaggcttaa ctgctgtaga gaaaatattc aataaaaatg
     3601 cagtgggcgt aactccagga aaagtattac acgcaggttc tgacgttcgt gtaaaagtaa
     3661 atattgttgg ttctcaagat acaactggtt tgatgactgc ccaagaatta gaatctatgg
     3721 ctgcgacggt tatctctcca actttagatg gtgcttatca atctggttgc catacagcat
     3781 cagtttggga taaaaaagct caggcaaata tcccaaaact aatgagcttt atgaacaact
     3841 ttggtttaat tacagcgaga gacccagaag gcgtttatca ttctatgact gatgtaattc
     3901 acaaagtatt aaatgatatc actattaatg attgggcaat tattattggt ggagactcac
     3961 atactagaat gtctaaaggt gttgcttttg gtgcagactc tggaactgtt gctttagctt
     4021 tagcaactgg agaagcttct atgccaattc cagaatctgt aaaagttact ttcaaaggtc
     4081 aaatgaaaga atatatggat ttccgtgatg tggttcatgc tacacaagca caaatgttac
     4141 aacaatttgg cggcgagaat gtattccaag gaagagtaat tgaagtacat attggcacat
     4201 tacctgctga ccaagcattt acatttactg actggactgc agaaatgaaa gcaaaagcat
     4261 ctgtaaatat ttcagaagat gcaacattaa ttcaatcatt ggagattgca aaaagtagaa
     4321 tccaaataat gattgataaa ggaatggata atgataatca agtattgaaa ggtttgattg
     4381 acaaagcaaa taatcgtatt gctgaaattc aatctggagt aaatcctgct ttacgccctg
     4441 atgcaaatgc aaaatatttt gcagaagtag tagtcgatct tgaccaaatt gttgagccaa
     4501 tgattgcaga tcctgatgta aacaataaag atgtttctaa acgctataca cacgatacca
     4561 tccgtccatt atcttattat gaaggcgaaa gaaaagttga tttaggattc gttggttctt
     4621 gtatggtaca caaaggcgac ttaaaaatcg ttgcacaaat gcttagaaat gtagaagcag
     4681 aaaaaggtaa agtagaattt aatgctcctt tggttgttgc cgcaccaact tataacatta
     4741 ttgatgaatt aaaagctgaa ggtgattggg atattttaga aaaatattct ggatttgaat
     4801 ttaatgatgc tgctccaaaa aatacggctc gtacagaata tgaaaatata atgtatttag
     4861 agcgtcctgg ttgtaactta tgtatgggta accaagaaaa agctgcaaaa ggcgatactg
     4921 ttatggcaac ttctacacgt ttgttccaag gtagagtggt agaagattca gcaactaaaa
     4981 aaggagaatc tttattatct tcaacaccag tggttgtact ttctgcaatc ttaggtcgta
     5041 caccaaatat tgaagaatat caatctgctg taaaaggtat taacttgaca aaattcaaac
     5101 cttcatcaat gcaattaact aaataattgt attgatgata caataaaaaa accgccactt
     5161 ttttggcggt ttttttattg atattccttt cctataaatt atgctccgcc tgttccttta
     5221 aaataatcat ctttatattt aaacaaaaca ttgaacgtcg ttaatgaacc ataaattcta
     5281 gtaatatatt gttgtaattc aactttctct gcatcagtca atttactagc attgattttt
     5341 tgttccatca ctcgcaatct atctcttgtc atcacaattt tatggaagaa agattctagg
     5401 tcaatttctt tatcttgcaa agatgtatca gccgattgaa tgattaattt tcctccttga
     5461 tatctatcgg caattttaca atcctcaatg ggttcaatta aaggctttat catttgatag
     5521 atggtcttct ctacttcgtc taatgaaata cctgaattgt ctttttctac taacttaata
     5581 accaacattc catcaaaggc ttttgaaatt tccttttcac cgtgttccga aaaataaata
     5641 ttatataaat attcatcttc attgtcaata attacacctt ttccaaaagt gggatgttct
     5701 aactttgttc ctatattata atttgtcata tttatatttt tggacgaaaa taaggaaaaa
     5761 caagcaagta aataaatttt tacacaatat tgtttttgag agccaagacg agatagaatt
     5821 aaataattta ttaattatta accactaaaa taatcctgtc catccttaat tccagtaaat
     5881 cctgattcag acaattaccg agaatcaaga ttactagagc caagaatcaa gactttattt
     5941 agacataagt agaaataatt aagaagtgag aattaaaaaa tcctgtctat catttaatcc
     6001 tccttcgcca ttgttggttg caaccaacaa tggcgagagg aataaagaac ttagtcaaaa
     6061 gctaaaaaca cctttcgagg gcgatggaga taataattat taaggttcag ttatgaagct
     6121 ttcttaaaac aaatcgaaat gtgttcctgt tcttattaat gtaacaagtt taataacctc
     6181 atcttgtttc catatcaaca accaatccga ctggatatga cattcccaat atcctttcca
     6241 attgcctgat aaaatatgag ctttatattt tgcagggagt tttccatcat tttcgagata
     6301 gataactgct tccttaaaaa gttccatatc cagtcctctt ttttgagcca acttaatatc
     6361 ttttttaatc tttccagaat attctatttc gtagctcata tcaccaatcc taaaaacaaa
     6421 tctttagaat tatttacttt aaaggttttt cctttttcta catcttgaat tgctttgatg
     6481 gttgcttcat ttggaacttt ttctttaatg atttctatac ctttattttc ggaaaccccg
     6541 attttaatca attctagcaa tgcttttccg tatttggtac gtttgtttat ttttaattta
     6601 atagcttcca taagttctta ttttatacaa agacaagaga aaataaagaa attaaaaaaa
     6661 acttaactcc taacttcaga caagcattat tccccaataa tttttattta taaaatagca
     6721 aataaggtat ttagtcataa ggtggtaact ctaattggtt tgttggttgc aaaccaacaa
     6781 gggcgaagag agtgtcctcc tttcctattg tccattatct atatttttaa gcgagaataa
     6841 ggattttaaa ctatctaaat tcatttttta aagtatatta cttaatttta caatatgcaa
     6901 aatccgacac catatcagcc taaaaacaaa attagaatag taaccgcagc ttctcttttt
     6961 gacggtcatg atgttgctat caatattatg cgtagaatta tgcagtcttc gggtgctgaa
     7021 gtgatacatc ttggacacaa tcgaagtgta aaagagattg tggactgtgc cattcaagaa
     7081 gatgcaaatg ctattgctat tacatcttat caaggtggtc atgttcgctt cttgaaatat
     7141 atgtatgatt tgttgcaaga gcaaaatagc aatcaaatta aaattgttgg cggtggtgga
     7201 ggaactattt taccatcaga aattgaagaa ttagaacaat atggcataga taaaatttat
     7261 tcgccagatg atggtcgtga aatgggtttg caaggtatga taaatgaagt attagaaatt
     7321 gcagatttcg cgacaggaaa agatattagt ttggatactg aattattaaa acaaaaaaat
     7381 accaaacaaa ttgcacaact aatttcttgt gctgaaaatt tccctaagga atttgaaaaa
     7441 acagcagctc aaatctcatc tctcactgct cacaatcaaa ctccaattct cggaattact
     7501 ggaactggcg gtgctggaaa gtcatctcta accgatgaga tagtacgcag atttttatta
     7561 gattttccag ataaatcagt ggcgattatt tccattgacc catcacgaag aaaaactgga
     7621 ggagccttat tgggcgatag aatccgaatg aatagtatca ataatgaacg tgtttttatg
     7681 cgttcgatgg caactcgtca aaccaatgct tctatttcta aagatgttca agatgctatc
     7741 cagattttaa aagctgctgc ttatgattta atcattgttg aaacttctgg aattgggcag
     7801 agtggtactg aaattattga tattgctgat gttttcttat atgtgatgac acctgaattt
     7861 ggagctgctt cacaattgga aaaaattgat atgttggatt atgcagatat tgttgctatc
     7921 aataaatttg ataagcgagg agctcaagat gctttaagag atgtcaaaaa acagtaccaa
     7981 cgcaatagaa acctatggga taaaaatcct gatgaaatgc ctgtttttgg tagtattgca
     8041 gcccaattta atgatccagg aactaatgaa ctatatctaa atctaattca aacaatcatt
     8101 acgaagacaa aagtagattg gcgtagtcaa ttaaatttaa aaactggaaa ttctgaaaaa
     8161 atctatatta ttccaccaaa cagaacacgc tatttatctg aaattgtaaa gacaaataaa
     8221 gattatgatg tttgggtcaa tgaacaagct gaaattgcaa acgcacttta tgctttggat
     8281 agagttcagt ctattgttca gagttcacag tccaataaga accaagaatc aagaaccaag
     8341 aaccaagaac tattaccgat tgaccaatta aggaatgaac aacaacaaaa actgcatcat
     8401 gattgtaaac aaattttaga caattggaca gagaaagttc aatcttataa aaataaagag
     8461 tttgtattta aagttcgtga taaagaaata aaaattaaaa cacataccac ttcattatcg
     8521 catcaagaaa taccaaaagt ggcacttcca aaatacacag cttggggtga tattttgaaa
     8581 tggaatttgc aagaaaatgt accaggtagt tttccatata ctgctggtgt ctttcctttc
     8641 aaaagagaaa atgaagatcc tacaagaatg tttgctggag aaggtggtcc tgaaagaacc
     8701 aataaacgtt ttcattatgt gtctttaggt ttgcctgcaa aaaggttatc aactgcgttt
     8761 gatagtgtta ctttatatgg caacaatcca gatttacgac ctgatattta tggaaaaatc
     8821 ggtaattcag gcgtttcaat ttgtacgcta gatgatgcta aaaaactgta ttctggattt
     8881 gatttatgca gtccgacaac atcagtttct atgacgatta atggaccagc accaacaatt
     8941 tgtgcatttt ttatgaatgc tgcaatcgac caacaatgtg aaaagtatat caaggaaaat
     9001 aatttaacta caatagtaga acaaaagatt gatgcaattt ataaagcaaa aaatgtcact
     9061 cgtccttgtt attatggaaa attacctgaa ggtaatgacg gtttaggttt gatgctttta
     9121 ggcgttacag gcgaccaagt tttagaaaaa gatatttatg aaaaaataaa aaaatataca
     9181 ctttctcaag ttcgaggaac tgtccaagct gatattttaa aagaagatca agcacaaaac
     9241 acttgtatct tttctacaga gttttcatta aaattgatgg gtgatgtaca aagctatttt
     9301 gtagaaaata aagtacgtaa tttttattct gtttcaattt ctggttatca tattgccgaa
     9361 gctggagcga acccaatatc tcagctggca tttacattag ccaatggctt tacatttgta
     9421 gaatattatt tatccagagg aatgcatatc gatgattttg caccaaactt atcgttcttt
     9481 ttctccaatg gtatagaccc agagtatgct gtaataggtc gtgttgctcg aaaaatatgg
     9541 gcaaaagcaa tgaaatattt gtacaaaggc gatgaaagaa gtcagaaatt aaaatatcat
     9601 gtacaaacat ctggaagaag cttacacgct caagaaataa gttttaatga tattcgtaca
     9661 acattgcaag cattgtatgc tatttatgat aactgcaatt ccttacatac caatgcttat
     9721 gatgaagcaa ttaccacacc aaccgaagaa agtgtgcgta gagcaatggc aattcaatta
     9781 attatcaata aagaattagg gttggcaaaa aatgaaaatc ctttacaagg ttcttttatt
     9841 attgaagaat tgactgattt ggtagaagaa gcagtttata cagaatttga gagaatgacg
     9901 gagcgtggag gtgttttggg agcaatggaa actatgtatc aacgtggaaa aattcaggaa
     9961 gaaagtatgt attatgaaaa cttgaaacat acaggaaatt atccaattat tggtgtaaat
    10021 acttttttat cctcaaaagg ttcaccaact gtaattccag atgaagtgat aagaagtacc
    10081 gatgaagaaa aaaataatca gattgataat ttgaatgcac tgcatcaacg aaatgaaaat
    10141 gattcgataa agcttctaaa aacattacaa caaacagcat tagaaaataa aaatgtattt
    10201 gaagtcttaa tggaaacagt aaaatattgc agtttaggac aaattactca agcacttttt
    10261 gaagttggtg gtcagtatag aagaaatatg taggcattga aataagcagc ttgttgatat
    10321 caaccaacaa tatgcttatt ctatcaaaga ttctaaatta atgcagggcg ttcactaatt
    10381 tcatggtaac atattacatc ttttgtgaaa tagtgagtta ccatactttt cctcgttaat
    10441 ttagggtttt gaataggtaa gcctctatga attaagttgc tatgccaaat taacacatct
    10501 cctttctttg cagttagaat tgaaggttta agatgattgt cttttatcaa tttcgctgtt
    10561 ttaatttcat gttttaaatt tgcatttgca tcaagaaaaa gaaaattatt ttttgctcca
    10621 atatctttat tggttaagta gggtaattga tgagaacctg gataatatgc tatcgctcct
    10681 gcttctaatt gaatatcttc taaagcaatc caagccgcaa ttaaatttcc ttgtggatat
    10741 gtactcatgt gaatagcatc actatgcgga gcttgttcac taccttgaat aaaatttata
    10801 gtttggaacg ggattactgg gttttctaaa ataaattcta aaattgattt tagtcttgaa
    10861 tcttgaattg tttggcgtat aatttcagaa tattgaaaag cattcattac ctttcttttg
    10921 gtataattaa attgcaacat ttcagtttcc aatagcaatt caatttcttg attaatttta
    10981 tcaattatgg tgtccttata aaatgaaggc aaaactaaaa aaccattttt atgccaatct
    11041 aacaaagctg attgaacaga tttgtcgaat tgcatataaa ctggatgttc cactaatttt
    11101 tctggtgtaa caggatagtc caaccaaggt aatcgattgg gtttgcctat aaaatctttt
    11161 gaagatatag atgaatatac agatttattt ataccaaatc ttttatataa atctttatta
    11221 cgtttgagtt tttgtcgatt gatataatta taaataaaat gcaacaaacg caggtttcgc
    11281 caaaagccag tatatgtttg tatccatttc atttagacaa caagcctatt ttttattaaa
    11341 ttgagcgtat aagcttgcag ccataatact tgccatcttc aattctttct gatttatatt
    11401 agttggatgg aaataattat caacaaattt tgtatcaaaa tcgcctgaaa taaaagcagg
    11461 gtggtttaat acaaattttc caaaacctaa agttgtttta actccttcaa ttttataatc
    11521 atcaatagca tttaataatt tttggattgc tgcatctcga ttttctgcat gagcaattaa
    11581 tttggcaatc attgggtcat aataaattgg gatttccata ccttcatcaa aaccatcatc
    11641 aactcgcaca ccatcaccag ttggaacttg atatgtatcc aatgttccga tatctggtag
    11701 aaaattattg tttggatctt cagcatatac acgcaactca attgaatgtc cattaatttt
    11761 taaatcctct tgtgaaaaag aaagtggctt cccttctgca atttgaattt gttccttaac
    11821 taaatctttt ccagtaatca tttcagtaac tggatgttct acttgcaaac gagtattcat
    11881 ttccaagaaa tagaaatcta aattattatt ggcgataaat tctacagttc ctgcacccac
    11941 ataatttact gctttggcaa cattcactgc tgcttctccc attttcttac gaacttcagg
    12001 tgttaataaa gctgatggag cttcctccac aaccttttga tgtcggcgtt gtacagaaca
    12061 ttctcgttca aataaataaa cgacattacc atgtgcatcg cctaaaactt ggatttcgat
    12121 atgtcgagga gattgtacaa acttttcaat aaaaacagca ccatcaccaa atgcagaaac
    12181 agcttctgaa acagctaagt tcatttgttc ttcaaattct gcttcatttt caactacacg
    12241 catacctttt ccaccgccac ctgcactggc tttaatcaaa attgggaatc caatttcggc
    12301 tgctaatttt ttagcttcag caatatctgt aatgggttgg tctgttccag gtactaaagg
    12361 cacattatat tttccgacag cttgtttagc tgctaattta ctgcccatca cttctattgc
    12421 caatacacta ggtcctataa aaacaatacc attttcttca cacgcttttg caaactgagc
    12481 attttcagaa agaaagccat aacctggatg tacaccatca acattgagtt gcttgcatac
    12541 ttttatgatt ttatctatat ccaaatagga ttgcttagat gcaggttctc caatgcaaac
    12601 cgcttcgtca gcaaagcgta catgaggtga ttttctatct gctgtagaat atactgcaac
    12661 tgtagctatt cccatttttt tacaagtacg cattactctc aatgcaatct ctcctctatt
    12721 agcaactaat atttttttca tcaaggctaa aatatgaaaa tatatagaat tatctttgca
    12781 atcgcatgga tttgcaaatt atttatattt tgttaaaaaa ggagttttta ttggaatggc
    12841 gttcaaaagc atcaatagga agcttattgg tacacgcatt agccacgatt ttttttattt
    12901 atatgatatt taatggagat atcaacttca ttacttggtt agcacttttt tgggtaatta
    12961 atttattttc tgttattcaa attacagcac gaacattcca aaaagatgcc aatgaacatt
    13021 tttattattt aaaacaacta gcctctgcac aacatattat actgtctaaa attatataca
    13081 atacgatatt tattatcatc atatccttca ttgtatttct atttatgcaa ttattctttt
    13141 cacagcaagt tacctacttg ggtatttttt cattaactat actattagca tctttaggct
    13201 tttcggcatt attttcatta ctatcaggca tttcagctaa aacacaaaac atggttttat
    13261 tggcagtttt gggcttacca attattttac cactcatttt aattatttca aaactaagtg
    13321 cattagcaga tttctacacg actattaacg atatttatat ttttttaagt gctgctgtgc
    13381 tattagatat tacaatttta atacttgcta tcatcttatt tccttacctt tggaaagatt
    13441 aatttttgga aattatgaag caaaattggt ggaaatttgt ggcgagtata tttttgatat
    13501 atagtttgat tgctggattt ttattaccta ttccagaatt aaatatttta gaacagtcta
    13561 tacgtaattt atttttccat gttccgatgt ggttttctat gatggcgatg atgtttattt
    13621 ctttgtggta tagcattaaa tatttaaact ccaacaaaac catttatgat ataaaagcga
    13681 gtgcatttgc acaagttggc tttgtacttg ggttattagg cataacaaca ggttcagttt
    13741 gggcaaaagt aacgtggggt gcttggtggg ttttttcaga acccaaatta aatggggcag
    13801 cagcagcttt attggtttat gctgcatatt ttatactccg aagctcgttt gatgatgaag
    13861 aaaaaacagc tagatttagt gccgtatata acttatttgc ttttgtaatg tttatggttt
    13921 ttatcaatat tttaccaaga ttaacagatt cattacaccc aggtaatggt ggaaatcctg
    13981 gatttaatgc ttatgattta gatagcagac ttagagccgt tttttatcct gcatgtatag
    14041 gctggatagc cattgcctat tggataggaa atattgtagt acgtattaag caattaaatc
    14101 gcaaaatttt aatgctcgaa atagattaga agacttatga atttgaagcc ttatttattt
    14161 tttatatttg tattttattt tgtttttttg aatggagctg aaaatggcta caaatattgg
    14221 aagaaagcca atcaattatt tgaacaaaag aaatatcaag aagctttaat ttattatgat
    14281 aagatagata aaaatattga tatcctagat gtaaagatta attcagctcg ttgcttatat
    14341 ttcttagaga aatttgttga aggcgaaatt gcctatcaaa aaattataga aaccccaaat
    14401 attaatttag atgtttataa agaatatgcc aacattttaa tcaaaaatgg taaagctaat
    14461 gaagcaaaaa tatgggataa aaaatatatt gaggaaaaca taaaacaaca aaaatttatt
    14521 accgaaattc caaagaatcc aactaaagta gatagtagca agacgaaagt agtaagtagt
    14581 aagacggata aagtgcaaaa attaaaaccg attaaggaag atagtagtaa gactaaagta
    14641 gtaagtagta agaatcaagt agtaaataat aagattgata aggagcaaaa attaaaaccg
    14701 attaaggata ctgtaaagat tgaaaaacct aaagcaataa agcaaaaaga tacaactgct
    14761 aaaacaatta aaacaccgaa agttaaacca actaaagtag atagtagtaa gacaaaagta
    14821 gtaagtaata aga
//
LOCUS       DAOQCW010000063        14281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1565_length_14281_cov_5.369535, whole genome shotgun sequence.
ACCESSION   DAOQCW010000063 DAOQCW010000000
VERSION     DAOQCW010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14281
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1565_length_14281_cov_5.369535"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..373)
                     /locus_tag="PLS16_06950"
     CDS             complement(47..373)
                     /locus_tag="PLS16_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60855.1"
                     /translation="MKLIFFLLAAQIIIFSATPCSDLSTYLAKESIHQTSSNSHENHG
                     HHDDCSPFCSCTCCTTGTIAQSDFYYDVVLTDAHNFYTDLVKIPSTEMNFVSSFYGDI
                     WQPPKI"
     gene            593..1984
                     /locus_tag="PLS16_06955"
     CDS             593..1984
                     /locus_tag="PLS16_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HNJ60856.1"
                     /translation="MRDRYIDLIEQTYYFPQDGFDIEDNWLNFHKIDLRQIIEEHGAP
                     LKIYYLPKIGDNINRARRWFYEAFESTGYNGHYYYCYCTKSNHFAFVLDEVLKNNAHL
                     ETSSNYDINLIRALFKKKKIDKKIRIICNGFKPKEYADSIVGLINDGYSNVIPVLDNM
                     DEWEYFKNVDKEINIGVRMAAEEEPNYQFYTSRLGIRQRDMIPFYIEKIKPNENVNLK
                     MLHFFIDSGIKDSAYYWSELNKCLNMYVDLKQVCPELNSINIGGGMPIRYSLGSKYDY
                     AYLIEEIVRKIKSFCDEAEIEHPNIYTEFGNFTVGESACNIFEVIGQKKQNDSELWYM
                     IGGSLMATIPDAWGLNQRFILLPINGWNQTYLRTIIGGLSCDVSDYYNSEVHINQVYM
                     PKIVGDQKLYIGFFHTGAYQDSISGYGGTKHCLIPSPKILIMDKDSNGKLTQRIFAEE
                     QSADSMLKVLGYY"
     gene            2094..2867
                     /locus_tag="PLS16_06960"
     CDS             2094..2867
                     /locus_tag="PLS16_06960"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE N-terminal
                     domain-containing protein"
                     /protein_id="HNJ60857.1"
                     /translation="MRHFNRPLVLLVLFVLATYSCANLKNNASTETPDGHTAQNSLDW
                     QGTYSGVLPCADCEGIETELKLNNDQTYSLTNKYLDGKNSSTDTLTGTFIWKGNNVEL
                     ERKIKDIRPDVFKVEENQVKQLDLEGKEVTGELANAYILKKNGNSEVEDKKWKLVELN
                     GKPVSGTADTHYIIFHSKEGRLEAKAGCNMMSYEYSIKNQLQVRFTPGITTLMACQET
                     TEDKLKAVLDKADNISVGPDVLTLNKARMAPLARFELVK"
     gene            2885..3286
                     /locus_tag="PLS16_06965"
     CDS             2885..3286
                     /locus_tag="PLS16_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60858.1"
                     /translation="MKTSPIFIMKPSILILLFIFTTLNINAQNNNCKNTLDSNSVVTL
                     LKSKGLILQNKQLSQEEIDQTPSYQPNVKFNELECTWEVSSQQYSTTKKGTCKHTNGC
                     TLVKSLLVIVDAQTQKIIKKYRTKELSPNFE"
     gene            complement(3287..3982)
                     /locus_tag="PLS16_06970"
     CDS             complement(3287..3982)
                     /locus_tag="PLS16_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNJ60859.1"
                     /translation="MVNKEALSHLIQVTKDKNVKLIAISKTKTEKDILEVYNAGQKVF
                     GENKVQELVQKYEKLPKDIEWHLVGTLQTNKVKYIAPFVHLIHSVDSLKLAIEINKQA
                     EKNNRIIDVLLEVFIAAETTKHGFSEEELIEHLDIHAFESLKNIRIIGLMGMATFTQN
                     QIVIKNEFAGLKDLFERLKATYFKDNSAFTELSMGMSGDYEIAIDKGSTMVRVGSAIF
                     GERERKKGVNVPL"
     gene            3988..4488
                     /locus_tag="PLS16_06975"
     CDS             3988..4488
                     /locus_tag="PLS16_06975"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HNJ60860.1"
                     /translation="MRFITLKNTSIFCFILLLLFSCDKNKAKKGISEENFKSIMIDLF
                     LIQGTYDYQQGSIYNDSTKNALDSIFNLVYKKHHTDSTIFKESWDYYLSDKNNYLSLM
                     SAIRDSIQKFDSVAQLRPINKDEETASYGASMEKEEQLFEQQFRGRKNLNRIRDSILK
                     QEKIKP"
     gene            4485..5456
                     /gene="bioB"
                     /locus_tag="PLS16_06980"
     CDS             4485..5456
                     /gene="bioB"
                     /locus_tag="PLS16_06980"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HNJ60861.1"
                     /translation="MTFDQIKEIYNRPLLELIYQSSDIHRKNHQAGEVQVCTLLSVKT
                     GGCTEDCAYCPQAARYHTDVKVHKLLDPEDVLNRAKEAKANGSTRFCMGAAWREVRDN
                     RDFDNVLAMVREVNKLDMEVCCTLGMLSASQAEKLKEAGLTAYNHNIDTSEEYYDKVI
                     TTRTYEDRLETIHNVSKAGISVCSGGIIGLGEEEDARIGMIYTLANLPEPPESIPVNA
                     LVPVKGTPLENMERVSVWEMLRMIATIRIVVPKAMIRLSAGRNEMPISEQALCFIAGA
                     NSIFAGDKLLTTPNPAFTSDMEMFEILGLQPRVSFKNENKNEVAEVL"
     gene            5456..6589
                     /locus_tag="PLS16_06985"
     CDS             5456..6589
                     /locus_tag="PLS16_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HNJ60862.1"
                     /translation="MSTIETQLKVYLEKRKENQTFRTLYTNSNLIDFSSNDYLGLANS
                     AWVKQEIYKDLATNYRYAKSGATGSRLLSGNSKLYESLEQQLADFHHAESALLFNSGY
                     NANLGLIATIARKNDLILYDELAHASILQALKISVATSEKFAHNDLIHLQELLETNQN
                     KYNQIFVITESLFSMDGDFAPLKDLALLSDKFNANLIVDEAHATGIYGKNGAGKCIEL
                     GIEKLCFARVYTFGKAIGAHGAVVVGSELLKNYLINFSKPFIYSTALSLYDLLHIKHS
                     YRFIQQFAFASNNLQNNIKYFKENISVHQNIPKIIGEGPIFAYIIEHSEICRAKAKQL
                     QSKGLDIKPIVYPTVAKRKERLRIIIHSFNTKEQIDRLIHSIL"
     gene            6723..8351
                     /locus_tag="PLS16_06990"
     CDS             6723..8351
                     /locus_tag="PLS16_06990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HNJ60863.1"
                     /translation="MTFSAKNRSEYIKTLKTEQFDLVVIGGGITGAGIALDAATRGLK
                     VALVEKQDFAFGTSSRSTKLIHGGLRYLKQLEFGIVHEVGRERAILYKNAPHIVIPEK
                     MLLPIIKGGSLGKRSTSLGLYVYDKLAGVNRKERRFMLDKSKTLAVEPLLNESILRGG
                     GMYVEYRSDDARLTIEVMKTAVANGALCLNYIECTDFRLDEKNKISGIAVVDKLKNEE
                     FIIQTKKVINAAGPWVDNLRKIDLSLKGKRLHLTKGVHIVIPHKRMPISHATYFDVAD
                     GRMVFAIPRDNIVYLGTTDTNYKEDTNFPYAEKEDVQYILDAANNMFPSLKLTMKDVI
                     STWAGLRPLIHEDGKSPSDLSRKDEIFISESNLISIAGGKLTGFRKMAQRSVDVVCNQ
                     LKKEEGRLFQPCSTEFIQLSGGNLEGKSIEEYARNLQTQFKDFDLDKVTALVKKYGSN
                     TKEILEITESKTNNDLLLGEAIYSMNEEMTYNIADFYIRRTGRVYFERNNLEPIIDKI
                     HQFFKDQLYYDSETALQFREDFNKEYEGVVQFKS"
     gene            8369..9340
                     /locus_tag="PLS16_06995"
     CDS             8369..9340
                     /locus_tag="PLS16_06995"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HNJ60864.1"
                     /translation="MEILDKQQRLIKDEKLLPFGIYNDLINDVSTSFYDKQQFSLRRK
                     TERKTWIFFGFYSKEITCGFAIVDAGIAATAFSYIFIPEENIFAEDKTTIPFGFDGNF
                     NPNLYDTWVLKNYSIHSNKSKMFYETTNKKFELNIRANNNDNGFSVVAPSKDKRPFNF
                     TYKNLMIDSEVEVKYNSKTYNAKGNFGAIDFTKGYPPRETMWNWLSFIGKTTSGKTIA
                     ANIVDNFNDNLENILYVDGIKTVLSKSNFLYKKPMDKNVWKINTLDNLLHLEVHPLGK
                     RSEDLNLMFMKSKFTQIFGEIKGDVRIDGKLENFTAVGVTEEHFALW"
     gene            9345..9842
                     /locus_tag="PLS16_07000"
     CDS             9345..9842
                     /locus_tag="PLS16_07000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HNJ60865.1"
                     /translation="MIEKLKHINTFIFDVDGVLTNGMLIGTDNGELWRLFNAKDGFAL
                     KVALQKNYNLCIITGGTSIGVYKRFEQIGFKHIYYKIKDKLPVLKDFLEQQNIKAEQV
                     MYIGDDLPDYHAMHYCGISACPNNAATEIKATVDYICKTNGGEGCAREIIEMVLKSQD
                     NWYKL"
     gene            9850..10836
                     /locus_tag="PLS16_07005"
     CDS             9850..10836
                     /locus_tag="PLS16_07005"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HNJ60866.1"
                     /translation="MNYIKLIRPINILFIIFIMILFRYAFVLPNFYKVYDFSSELSTF
                     QFILLLLVTVFTAASGYVINDIYDSDIDLVNKPKKVIIDKTIGVDDAYNFYKILALIT
                     ILLSVTLTISTKNYGLATIPILILVALNFYAQIFKKLFLIGNLIIALFSAMVILLPAL
                     YESKTNLDTPKIVIDIKSGILTAAICYALFAFFTTFIREIVKDMQDIEGDKQYGCKTV
                     PIILGSTKSKILISVIAIFLLLFIFSFAQFFPSLNIQYIHLYIDIILILPFIFIIALL
                     WWGKTNNHYRLVSGALKTYMLIGVCTMFYFVYVSGAASYLFVQYANFIKKIF"
     gene            10839..11402
                     /locus_tag="PLS16_07010"
     CDS             10839..11402
                     /locus_tag="PLS16_07010"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HNJ60867.1"
                     /translation="MKDKIILGSQSPRRKVILEEAGFDVQILLADIEENYPDDIEKAD
                     VPEYLAKLKMQHLPCNLDENIFIITADTMLLFKDKLIGKPENTDEAFNMLSELNDTFH
                     EVITGVCIKKNNKTISFSQKTKVYFKKMNLEEIKNFIDTHIVLDKAGAYNIQEYIGVD
                     KIEGDFDNVAGLPIQTILEKIKNWNNL"
     gene            11433..11615
                     /locus_tag="PLS16_07015"
     CDS             11433..11615
                     /locus_tag="PLS16_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60868.1"
                     /translation="MKLKYLLPLITWIVPTVIISAIMFKVDAPLTDTQFGGFVALLLS
                     ACITYIVGIRITLKDK"
     gene            complement(11659..12138)
                     /locus_tag="PLS16_07020"
     CDS             complement(11659..12138)
                     /locus_tag="PLS16_07020"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ60869.1"
                     /translation="MIRIATILDIDKIIEIAEQSFKIHYKDIISQEQIDYMYEKMYAP
                     QAILQQMKDGHTFIIFFENENPLAFASYFIREDNCLYLSKLYIKYDFRRKKIGSQLLL
                     FIENIVHKNAYSFIELNVNRKNNAMYFYKSLGFIMHEKVDIAYSKFWLNDYVLRKYF"
     gene            complement(12206..13312)
                     /locus_tag="PLS16_07025"
     CDS             complement(12206..13312)
                     /locus_tag="PLS16_07025"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNJ60870.1"
                     /translation="MNKIISLLFVVLISLSVTARNNFSQNDASGHSIGITIGPSWALT
                     DLGGGGNSNPFLRDLDFKSTKFGIGAFYRYTINEWVAVRAQFLYGMLAGSDKNVKGPN
                     TYNGNYKGPFFRKARNADFKTHLLQIEAIAELNLKRYDPSASKGDKARWAPYLAGGFG
                     MFYFNPFTKGTFSTQNLGTATYPVGLGGGNVYTAAEINELQQYEGEKIKLRKLGASTG
                     STYAPISFNLTGILGLKFNVTDKIAIFGELWYNQTFTDNLDDVNGYYPNFSTYQGLSP
                     LQKAMTVRYYEIHNTLDPAYAYGGGSIFNPVTGEGYKRGDDDKDPGDAKGKNDQFFSA
                     QIGVSIRLEGGGRNNGFGCGRKNAYNHKFSCPKW"
     gene            complement(13368..14000)
                     /gene="nth"
                     /locus_tag="PLS16_07030"
     CDS             complement(13368..14000)
                     /gene="nth"
                     /locus_tag="PLS16_07030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNJ60871.1"
                     /translation="MTKKEKTAFIVNTLEQLFPNPPIPLHHKDAYTMLIAVLLSAQCT
                     DERVNKITPLLFKKADNPIKMSKLSVEEIRDIIKPCGLSPQKSKAIHQLSHILIDKHQ
                     GNVPNSMELLEELPGVGHKTASVVMSQIFGEPTFPVDTHIHRLAYRWGLSDGSSVQQT
                     EKDLKKTFDKNLWNNLHLQIIYYGRSYCRARPHDVKNCIICQKIGVKSRM"
     gene            14037..>14281
                     /locus_tag="PLS16_07035"
     CDS             14037..>14281
                     /locus_tag="PLS16_07035"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HNJ60872.1"
                     /translation="MIEIYTDGAARGNPGRGGYGVILKYKQQYKELSDGFRLTTNNRM
                     ELLACIIGLEAIKAQNMDITIFSDSKYVVDSVVKGWV"
BASE COUNT         5225 a         2061 c         2203 g         4792 t
ORIGIN      
        1 atttaaaatg ctctttactt tgaacagtat aatttattct aaaaaattaa attttgggcg
       61 gttgccaaat atctccgtaa aaactagaaa caaaattcat ttctgttgat ggaattttta
      121 ctaaatcagt ataaaaatta tgtgcatctg tcaagacaac atcataataa aagtcagatt
      181 gagcaatggt gccagttgta cagcaagtac agctacaaaa aggcgaacaa tcatcgtgat
      241 gaccatgatt ttcgtgtgaa ttagaagaag tttgatgtat tgattctttc gctaaataag
      301 tactcaaatc gctacaaggt gtagcactaa aaattattat ttgagctgct aacaaaaaga
      361 atatcagttt cactagaaca aagttaagaa taatttctat atatgtcatt aattaaagtt
      421 aattcatcat ttattaaata aaaatcaatt aaaaaaaaca ataattcagc aataaaaatt
      481 cacatgttaa aacacaataa ctaagcagga ttatagctaa aaaaaaaggt aataaataat
      541 aatattcttt attacctttg tgctttaacc aaatcgtttt tattagaaaa taatgagaga
      601 ccgttatatt gatttaattg aacaaaccta ttactttcca caagatgggt tcgatattga
      661 agacaactgg ctaaacttcc acaagataga tttaaggcag attatagaag aacacggtgc
      721 accattaaaa atttattatt taccaaaaat tggcgataat atcaatcgag caagacgttg
      781 gttttatgaa gcatttgaaa gcactggtta caatggtcat tattattatt gttattgtac
      841 aaaaagcaat cattttgctt ttgttttaga tgaagtttta aaaaacaatg cccatttaga
      901 aacatcttct aattatgata tcaatttgat tcgagcatta tttaaaaaga agaaaattga
      961 taagaaaatt cggattatct gcaatggatt taagcctaaa gaatatgccg attctattgt
     1021 tggcttaata aatgatggct attccaatgt cattcctgtt ttggacaata tggatgaatg
     1081 ggagtatttt aaaaatgtcg ataaagaaat caatattggt gtgagaatgg ctgctgaaga
     1141 agagccaaac tatcaatttt atacttcaag attaggtatc agacaacggg atatgattcc
     1201 attttatatc gaaaaaatta aaccgaatga aaatgtaaac ctaaaaatgt tacatttctt
     1261 catagactct ggcattaaag acagtgctta ctattggagt gaattgaata agtgtttaaa
     1321 tatgtatgtc gatttaaagc aagtttgtcc agaattgaac agcattaaca ttggtggtgg
     1381 aatgcccatt cgttattcat taggttctaa atatgactat gcctatttga ttgaagaaat
     1441 cgttagaaaa atcaaatcgt tctgtgatga agcagaaatt gagcatccaa atatttatac
     1501 tgaatttggt aattttacag ttggcgaaag tgcttgtaat atatttgaag tgattggtca
     1561 gaaaaaacaa aatgactcag aactttggta tatgattggt ggttcgttaa tggcaaccat
     1621 tccagatgct tggggtttaa atcaacgatt tattttattg cctataaatg gctggaacca
     1681 aacttattta aggacaatta ttggaggttt aagttgtgat gtaagtgatt attataactc
     1741 agaggtgcat attaaccaag tatatatgcc aaaaattgtg ggtgatcaga aattgtatat
     1801 cggttttttt catactggag cttaccaaga ttccataagt ggatatggtg ggacaaaaca
     1861 ttgtttaatt ccatcaccca aaatacttat tatggataaa gatagtaatg gaaaactaac
     1921 acaaagaatt tttgcggagg aacaaagtgc agatagcatg cttaaagtat taggatatta
     1981 ttaatatgta tttattcaat agttattcat ttacaaaaca acatatttta acaatcgaac
     2041 atgaaattca gcagtaattt tattctcata aatctatatt aataaatatg aaaatgagac
     2101 attttaacag accattagtt ttattagttc tatttgtttt agcaacctac tcgtgtgcaa
     2161 atttaaaaaa caatgcttca acagaaacac ctgatggaca cactgcacaa aattcattag
     2221 attggcaagg aacttactca ggagtattac cttgtgcaga ctgtgaaggt attgaaactg
     2281 aattaaaatt aaataatgac caaacttata gtttaacaaa taaatactta gatggtaaaa
     2341 attctagtac agatacattg actggaactt ttatctggaa aggaaacaat gtagaattag
     2401 aaagaaaaat aaaagatatt agacctgatg tatttaaagt agaagaaaat caagtaaaac
     2461 aattagactt ggaaggtaag gaagtaactg gagaattagc aaatgcatat attcttaaaa
     2521 aaaatggcaa ttctgaagtt gaagataaaa aatggaaatt agtagaatta aacggtaaac
     2581 cagtaagcgg aactgctgat acacactata ttattttcca ttcaaaagaa ggaagattag
     2641 aagcaaaagc aggatgtaat atgatgtctt acgaatattc tataaaaaat caattacaag
     2701 taagatttac tcctggtatc actaccttaa tggcttgtca agaaactaca gaagataaat
     2761 taaaagctgt tttagataaa gctgataata tttcagttgg tccagatgtg cttactttaa
     2821 acaaagccag aatggctcca ttagctagat ttgaattagt aaaataatat tataaataag
     2881 ggcgatgaaa acatcgccca tttttattat gaagccttca attttaatac ttttatttat
     2941 ttttacgaca ttgaatatta atgcacaaaa taataattgc aaaaatactt tagatagtaa
     3001 ttctgtagta acacttttaa aatctaaagg attaatacta caaaacaaac aactttctca
     3061 ggaagaaatt gatcaaacac cttcctatca acctaatgta aaattcaatg aacttgaatg
     3121 tacttgggaa gtttcctcac aacaatacag tacaacaaaa aaaggaactt gtaaacatac
     3181 caatggctgt acattagtaa aatcacttct tgttattgta gatgcacaaa cacaaaaaat
     3241 cattaaaaaa tatagaacta aggaattatc tccaaacttt gaatagttac aaaggtacat
     3301 tcacaccttt ttttctttct ctttctccaa aaattgcact acccactcga accatcgttg
     3361 agcctttatc tattgcaatt tcataatcac cactcatgcc cattgaaagt tcagtaaatg
     3421 cagaattatc tttaaaataa gttgctttca atctttcaaa caaatctttt aaaccagcaa
     3481 attcattctt aataacgatt tgattttgcg taaaagttgc cattcccatt aaccctataa
     3541 tacgtatatt tttcaaagac tcaaatgcat gaatatctaa atgctcaatc aattcttctt
     3601 ctgaaaaacc atgtttcgtt gtctctgcgg caataaatac ttccaataac acatcaatta
     3661 ttcgattatt tttttctgct tgcttattga tttcaatagc taatttcaaa ctatcaacag
     3721 aatgaatcaa gtgtacaaaa ggtgcaatat atttaacttt attggtctgt aaagtaccca
     3781 ccaaatgcca ttcaatatct ttgggtaatt tttcatattt ttgaactaat tcttgaactt
     3841 tattttctcc gaatactttt tgacctgcat tatacacttc taaaatatct ttttcagttt
     3901 ttgttttgct aattgcaatt aattttacat ttttatcctt tgtaacttga attaaatgcg
     3961 agagtgcttc tttatttacc ataactgatg agatttataa ctttaaaaaa tacaagtata
     4021 ttttgcttta tattgctatt gcttttttct tgcgataaaa ataaggcaaa aaaaggtatt
     4081 tccgaagaaa acttcaaaag tataatgatt gatttatttt taattcaagg aacttatgat
     4141 taccaacaag gttctatcta taatgattct acaaaaaatg ctttagattc tattttcaat
     4201 ctagtttata aaaaacacca tacagattct acaattttta aagaaagttg ggattattat
     4261 ttatccgata aaaataatta cctatcttta atgagtgcaa ttcgtgattc aattcaaaag
     4321 tttgattctg ttgctcaatt aagaccaata aacaaagatg aagaaactgc atcttatggt
     4381 gcatcaatgg aaaaagaaga acaattattt gaacaacaat ttagaggaag aaaaaatcta
     4441 aacagaataa gagactcaat actaaaacaa gaaaaaatta aaccatgaca tttgaccaaa
     4501 ttaaagaaat ttacaataga ccacttttag aactaattta ccaatcttca gatatacata
     4561 gaaaaaatca tcaagcaggc gaagtacaag tttgcacact actttctgta aaaacaggtg
     4621 gttgcactga agattgtgct tattgtccac aagctgcacg ttaccacact gatgtaaaag
     4681 ttcataaact tttagaccct gaagatgtat taaatagagc aaaagaagca aaagccaatg
     4741 gctctacacg tttttgtatg ggtgctgctt ggcgagaagt gcgagataat agagattttg
     4801 acaatgtttt ggcaatggta cgtgaggtaa acaaattaga tatggaagtt tgttgtactt
     4861 tgggtatgct atcagcttct caggcagaaa aattgaaaga agctggatta acagcctata
     4921 accataatat tgatacttca gaagagtatt atgacaaagt cattaccaca agaacttatg
     4981 aagacagatt ggaaactatt cataatgtta gcaaggctgg cattagtgtt tgtagtggtg
     5041 gtattatcgg gttaggcgaa gaagaagatg ccagaattgg catgatttat accttagcta
     5101 atttgcctga accgcctgaa tccattcctg taaatgcctt agttcctgta aaaggtacgc
     5161 cattagaaaa tatggaacgt gtttcagttt gggaaatgct aagaatgatt gcaactatta
     5221 gaatcgttgt tccaaaagct atgattagac tttcagcagg aagaaatgag atgcctataa
     5281 gcgaacaagc tttatgtttt attgctggtg caaattcaat ttttgctggc gataaactat
     5341 taactacacc aaatcccgct tttacttcag atatggaaat gtttgaaatt ttaggcttac
     5401 aaccgagagt ttcatttaaa aacgaaaata aaaatgaagt agcggaagtt ttgtaatgag
     5461 taccattgaa acgcaattaa aagtatattt agaaaaaaga aaggaaaatc aaacattccg
     5521 aactttatat acaaatagca atttaattga tttttcatct aatgattatt taggtttagc
     5581 caattcagct tgggtaaaac aagaaattta taaagactta gctacaaatt atcgctatgc
     5641 caaatcagga gcaacaggtt ctcgtttatt gagtggaaac tcaaaattgt atgaatcact
     5701 agaacaacaa ttagcagatt ttcatcacgc agaaagtgct ttattgttta attctggtta
     5761 caatgccaat ttaggtttaa tagcaacaat tgcacgcaaa aatgatttaa ttctatatga
     5821 tgaattagca catgcttcaa ttttacaagc actaaaaatt agcgtagcta ctagcgagaa
     5881 atttgcacac aatgacctga ttcatttaca agaattactt gaaacaaatc aaaataaata
     5941 caatcagatt tttgtaatta ctgaatcact attttctatg gatggtgatt ttgcaccact
     6001 aaaggatttg gcattgcttt ctgataaatt caatgcaaac ttaattgttg atgaagctca
     6061 tgcaactgga atttatggca aaaatggtgc aggaaaatgt atagaattgg gtatagaaaa
     6121 actgtgtttt gcacgagtat atacttttgg aaaagcaatt ggtgcacatg gagcagtggt
     6181 tgttggtagt gagttattaa aaaactatct tattaatttt tctaagccat ttatttattc
     6241 tacggcttta agtttgtacg atttgctgca tatcaaacat agttatcgtt ttattcaaca
     6301 atttgctttt gcttctaata acttgcaaaa taatatcaaa tattttaaag aaaatatatc
     6361 agtacaccaa aatataccaa aaataattgg agaaggacca atatttgctt atattattga
     6421 acattctgaa atatgcagag caaaagcaaa acaattacaa tctaaaggct tagatataaa
     6481 acctattgtt tatccaaccg tagccaaaag aaaagaacgt ttacgaatta tcattcatag
     6541 ttttaataca aaagaacaaa tagatagatt aattcatagt attttataat ataaaaactt
     6601 tgtaaatctc tatttaaaac ttatcctcta ttattattac atttttaaat tatcaaccaa
     6661 cactatcatt aaatctatgc ttttgattat ctttagataa attttaaaca agcaaaacta
     6721 ttatgacttt ttctgcaaaa aatcgctctg aatatatcaa aacgcttaaa actgaacaat
     6781 ttgatttagt agtcattggt ggtggtatca ctggtgccgg aattgcacta gatgcagcta
     6841 cccgaggttt aaaagttgct ttagtagaaa aacaagattt tgcttttgga acaagttctc
     6901 gatcaacaaa attaattcat ggtggcttac gctatttaaa gcaattagaa tttggtatcg
     6961 tacacgaagt aggcagagaa cgtgcgattt tatataaaaa tgcaccgcat attgtaattc
     7021 ctgaaaagat gttattgccc attattaaag gcggttcatt gggaaaacga agtacttctt
     7081 taggtttgta tgtatatgat aagttggctg gcgtgaacag aaaagaacgc cgttttatgc
     7141 tagataaatc taaaacacta gcagtcgagc ctttattaaa tgaatctatt ttgcgtggtg
     7201 gtggaatgta tgttgaatat cgaagtgatg atgctcgatt gacgattgaa gtgatgaaaa
     7261 ctgccgtcgc taatggtgca ttatgcttga attatattga atgtacagat tttagattag
     7321 atgaaaaaaa taagattagt gggattgcag tcgtcgataa attgaaaaat gaagaattta
     7381 tcattcaaac taaaaaagta attaatgctg ctggaccttg ggtggataat ttgagaaaaa
     7441 ttgatttatc attaaaagga aaaagattac accttaccaa aggtgttcat attgttattc
     7501 cacacaagag aatgcctatc agtcatgcaa cttattttga tgttgctgat ggacgtatgg
     7561 tatttgctat tcctcgagat aacatagttt atttaggtac aacagataca aattataaag
     7621 aagataccaa ttttccttat gctgaaaaag aagatgtaca atacatctta gatgctgcga
     7681 ataatatgtt tccaagttta aaactaacca tgaaagatgt tatttctact tgggcaggtt
     7741 taagaccttt gatacatgaa gatggaaaat ctccttcaga tttatccaga aaagatgaaa
     7801 tttttatttc tgaatccaat ctaatatcca ttgcaggtgg aaaactaact ggttttagaa
     7861 aaatggcaca acgctctgtt gatgttgtat gtaaccaatt aaaaaaagaa gaaggcagat
     7921 tattccaacc ttgtagtaca gaatttattc aattatcagg cggaaattta gaaggcaaaa
     7981 gtattgaaga atatgcaaga aatctgcaaa ctcaatttaa agattttgat ttagataaag
     8041 taactgcatt ggtaaaaaaa tatggcagca atacaaaaga aattttagaa atcactgaaa
     8101 gcaaaaccaa taatgattta ttattaggcg aagctatcta ttctatgaat gaagaaatga
     8161 cctacaacat tgcagatttt tacattagac gtacaggtcg tgtttatttt gaacgcaata
     8221 atttagaacc aatcatagat aaaatacatc aatttttcaa agaccagctt tattatgata
     8281 gcgaaaccgc tctacaattt agagaagact ttaataaaga atacgaaggt gtcgtacaat
     8341 tcaaatctta aaatacaaat tatataaaat ggaaatttta gataaacagc aacgtttaat
     8401 taaagacgaa aaactattgc cttttggtat ttacaatgac ttaattaatg atgtaagtac
     8461 ttctttttat gacaaacaac aattctcgct aagaagaaaa acagaacgca aaacttggat
     8521 attttttggc ttctattcta aagaaattac ttgtggattt gctattgttg atgcaggcat
     8581 tgcagcaact gcattttctt atatttttat acctgaagaa aatatttttg ctgaagacaa
     8641 aacaactatt ccatttggtt ttgatggcaa ttttaatcct aacttatatg atacttgggt
     8701 gctaaaaaat tacagcattc atagcaacaa atccaaaatg ttttatgaaa ccaccaacaa
     8761 aaaatttgaa ttaaatattc gtgccaataa caacgacaat ggctttagtg tggtcgcacc
     8821 ttcaaaagat aaaagaccgt ttaattttac ttataaaaat ctaatgatag actcagaagt
     8881 tgaagttaaa tataatagca aaacttataa tgcaaaagga aattttggag ctattgattt
     8941 tacaaaaggc tatcctccac gagaaacgat gtggaattgg ctttcattta ttggaaaaac
     9001 tacatctggc aaaacaattg cggctaatat tgtagataat ttcaatgata acttagagaa
     9061 tattttgtat gtagatggca taaaaacagt tttgtcaaaa tccaattttt tatataaaaa
     9121 accaatggat aaaaatgttt ggaaaatcaa tactttagat aatttattgc atttagaagt
     9181 tcatccatta ggcaaaagaa gcgaggattt gaatttgatg tttatgaaaa gtaagttcac
     9241 acaaatcttt ggagaaataa aaggagatgt taggattgat ggtaaattag aaaattttac
     9301 ggctgtcgga gtaaccgaag agcattttgc attgtggtag aattatgata gaaaaactaa
     9361 aacatatcaa tacatttatt tttgatgtag atggtgtatt aaccaatgga atgttgattg
     9421 gaacagataa tggcgaactt tggcgtttat ttaatgcaaa agacggcttt gctttaaaag
     9481 ttgcgttaca aaaaaattac aatttgtgta ttattactgg aggcacttca atcggtgtgt
     9541 ataaacgttt tgagcaaatt gggttcaaac acatttatta caaaataaaa gacaaactac
     9601 ctgttttaaa agatttttta gaacaacaaa atataaaagc agaacaggtg atgtatattg
     9661 gtgatgattt acctgattat catgctatgc attattgtgg gattagtgct tgtccaaata
     9721 acgccgctac tgaaattaaa gcaactgttg attatatttg caaaaccaat ggtggcgaag
     9781 gttgtgcaag agaaataata gaaatggtat taaagtccca agacaattgg tacaaattat
     9841 aataaaacaa tgaattacat caaattaata agaccaatca atatattatt catcattttt
     9901 ataatgattt tattcagata tgcttttgta ttacctaact tttataaagt atatgatttt
     9961 agttctgaat taagtacatt ccaatttatt ttactcttat tagttaccgt ttttactgca
    10021 gcaagtggtt atgtcattaa tgatatttat gattctgata tagatttagt aaacaaaccc
    10081 aaaaaagtaa ttattgataa aaccattggt gttgatgatg cctataattt ctataaaata
    10141 ttagcactaa tcactatatt actttcagtt acattaacaa tttctactaa aaattatgga
    10201 ttagcaacca ttccaattct gattcttgtc gccttaaatt tttatgctca aattttcaaa
    10261 aagctatttt taataggcaa tcttattatt gcattatttt ctgcaatggt cattttgtta
    10321 cctgcattat acgaaagtaa aacaaattta gataccccaa aaattgtaat tgacatcaaa
    10381 agtggaattt taactgctgc aatttgttat gcactatttg catttttcac aacttttatc
    10441 cgtgaaatag ttaaagacat gcaagatatt gaaggagaca agcaatatgg ttgtaaaaca
    10501 gtacctatta ttttaggtag cactaaaagt aagattttaa tcagtgtaat tgctattttt
    10561 ttattgcttt tcatttttag ttttgctcaa ttttttccta gcttaaatat ccaatacatt
    10621 catctgtata ttgatataat tttaatttta ccattcatat tcattattgc attactttgg
    10681 tggggaaaaa ctaataatca ttaccgatta gtttctggtg ctttgaaaac gtatatgctt
    10741 attggggttt gcacgatgtt ttattttgtc tatgtaagtg gtgcagcatc ctatttgttt
    10801 gtacaatatg ctaattttat taaaaagatt ttctaaactt gaaagacaaa attattttag
    10861 gttctcaatc tccaagaaga aaagttattc ttgaggaagc tggttttgat gttcaaatat
    10921 tacttgcaga tatagaagaa aattatcctg atgatataga aaaagcagat gtcccagaat
    10981 atcttgctaa gttaaaaatg caacaccttc cttgcaattt agatgaaaat atttttataa
    11041 taacggcaga tacaatgtta ttgttcaagg ataaattaat tggaaagcct gaaaataccg
    11101 atgaagcctt taatatgtta tcagaattaa atgatacctt tcatgaagtc attacaggtg
    11161 tttgcattaa aaagaataat aaaaccattt catttagtca aaaaactaaa gtttatttca
    11221 aaaaaatgaa tcttgaagaa ataaaaaact ttattgatac tcacatcgta ttggacaaag
    11281 ctggtgctta taatattcag gaatatattg gcgtagataa aattgaagga gattttgata
    11341 atgtagcagg tttacctatc caaactattt tagaaaaaat aaaaaactgg aataacttat
    11401 aagaagtcca tttttatatc taatttgcaa caatgaaatt aaaatatctt ttaccactta
    11461 ttacttggat agttccaact gtaattattt cagccattat gtttaaagtg gatgcaccat
    11521 taaccgatac tcaatttgga ggatttgtag ccttacttct ttctgcttgt atcacttata
    11581 ttgttggaat aagaattaca ttgaaggaca aataaaaaga cataaaaaaa catgtcaatt
    11641 atttttattt ggatacgcct aaaaatactt tcttaaaaca taatcattta accaaaactt
    11701 gctataagca atatccactt tttcatgcat aataaatcct aaagatttat aaaaatacat
    11761 tgcattattc ttacgattta catttaattc aataaatgaa taggcatttt tgtgaacaat
    11821 attttcaatg aatagtaata attgagaacc tattttcttt cttctaaaat cgtattttat
    11881 atacagtttc gataagtaca aacaattatc ttctctaata aaataggaag caaaagccaa
    11941 tggattttca ttttcaaaga atattataaa agtatgacca tctttcattt gttgtaaaat
    12001 agcttgtgga gcatacatct tttcatacat ataatcaatt tgctcttgag agatgatgtc
    12061 tttataatgt attttaaaac tttgttcagc tatttcgata attttatcaa tatctaatat
    12121 cgttgcaatt ctaatcattc aattattaat aaaaatggac actaaaaaat agtgtccaat
    12181 attttgtatt aaatttaaac caatattacc atttaggaca gctaaattta tggttataag
    12241 catttttacg tccacatcca aatccattat ttcttccgcc accttcaagt ctgatagaaa
    12301 caccaatttg tgcagagaaa aattggtcat ttttaccttt agcatcacca gggtccttgt
    12361 catcatcacc tcttttatac ccttctccag ttacaggatt aaagatactg ccaccaccgt
    12421 aagcataagc tgggtcaagt gtattatgta tttcataata tcttacagtc attgcttttt
    12481 gcaaaggcga aagtccttga taagtactga aattagggta gtaaccattt acatcatcta
    12541 aattatccgt aaaagtttga ttgtaccata attcaccaaa tattgcaatt ttatcagtta
    12601 cattaaattt caatcctaaa atacccgtta agttaaaact tattggagca taagtgcttc
    12661 cagttgaagc acctaatttt cttaatttaa tcttctcccc ttcatattgt tgtaactcat
    12721 taatttctgc tgctgtatat acatttccac cacctaaacc aacaggatat gttgcagtac
    12781 ctaaattttg agtactaaat gttcctttag tgaaagggtt aaaataaaac attccaaaac
    12841 cacctgctaa atatggtgcc catcttgctt tatcgccttt agatgcagaa ggatcatatc
    12901 tctttaaatt taattcagct attgcttcta tctgcaataa atgagtttta aaatctgcat
    12961 tccttgcctt tctaaaaaaa ggtcctttgt aatttccatt atatgtattt ggtcctttaa
    13021 catttttatc actaccagct aacatcccat ataaaaactg agctcttaca gcaacccatt
    13081 cgttaatcgt gtatctataa aatgctccaa ttccaaactt tgtagattta aaatctaaat
    13141 ctcttaaaaa tggattacta tttccacctc ctcccaaatc tgttaatgcc caacttggtc
    13201 cgatagttat tcctatagaa tgaccacttg catcgttttg agagaaatta tttcttgcag
    13261 taacagaaag tgaaattaat actacaaaca gtaaagagat tattttattc atctttatat
    13321 ttttaataga atttataaag taatggttaa agataattaa aaaaaaatta cattctactt
    13381 tttactccga ttttttgaca aattatacaa tttttcacat catgtggtct ggctcgacaa
    13441 taacttcttc cataatagat gatctgtaaa tgtaaattat tccataaatt tttatcaaaa
    13501 gttttcttca aatctttttc tgtttgctgt acgctacttc cgtctgatag accccaacga
    13561 taagctaaac gatgaatatg tgtgtccact ggaaaagtgg gttctccaaa aatctggctc
    13621 attaccacag atgcagtttt gtgtcctaca cctggcaatt cttctaataa ctccatagaa
    13681 ttgggtacgt ttccttggtg tttatctatc aatatatgag acaactgatg tattgcttta
    13741 gacttttgag gagacaatcc gcaaggttta ataatatctc ttatttcttc aacagaaagc
    13801 ttagacattt ttataggatt atctgctttt ttaaataaca atggtgtaat tttattgaca
    13861 cgttcatcag tgcattgggc agaaagtaag actgcaatca acattgtata agcgtcttta
    13921 tgatgtaagg gaataggagg atttgggaat aattgctcta aagtatttac aataaacgct
    13981 gttttttctt tcttagtcat gggactaatt tactattttt accgaaaata ataaaagtga
    14041 tagagattta tactgatgga gcagcaagag gaaaccctgg acgaggtggc tatggcgtta
    14101 ttttaaaata taaacaacaa tacaaggaac tttcagatgg ttttcgttta acgaccaaca
    14161 accgaatgga attattggct tgcatcattg gactggaagc tataaaagct caaaatatgg
    14221 atattacgat attttctgac tccaaatatg tagtggatag tgtagtaaaa ggctgggtgt
    14281 t
//
LOCUS       DAOQCW010000064        14041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1601_length_14041_cov_5.705491, whole genome shotgun sequence.
ACCESSION   DAOQCW010000064 DAOQCW010000000
VERSION     DAOQCW010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14041
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1601_length_14041_cov_5.705491"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="PLS16_07040"
     CDS             complement(<1..927)
                     /locus_tag="PLS16_07040"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HNJ60873.1"
                     /translation="MLFLAMFVSISKYAVFSLNQYQIAQIKKQQSKKYKILEQFIHKP
                     KQLLATYLYAITFFSFGIVILTENLFNTWFYSLNVVENRGWYLFLRLLILTLCLLFFV
                     EIIPRIYGKEHNLKWSQRLIYLVWFLNILFKPFTKLFISIAELIETQMENTSNSLTAE
                     DIDEAIDNTTADEHKKKDSQILKGLIKFGNITVSQIMRSRIDVVAVDKNIKLNQLLAM
                     VKDSGYSRIPVYEDSIDKISGIIYAKDLLLYLEETDSFDWQKLIKPAFFVPVSKKIDD
                     LLEDFQNKRIHMAVIVDEYGGTLGIVTLEDVLE"
     gene            complement(1042..1473)
                     /gene="ssb"
                     /locus_tag="PLS16_07045"
     CDS             complement(1042..1473)
                     /gene="ssb"
                     /locus_tag="PLS16_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNJ60874.1"
                     /translation="MSVNKVILIGRLGKDPEVRKLSTNATVCNFPLATKEFYKKEDGT
                     YLEQTEWHNIVMWRGVAERAERILKKGNNVFIEGKIKTRSWDDKEGNKRYSTEIVVEN
                     FQLLEKKQDGNVANADTQPGEHLDRPDTDLEQGIQNDDLPF"
     gene            complement(1508..2545)
                     /gene="mutY"
                     /locus_tag="PLS16_07050"
     CDS             complement(1508..2545)
                     /gene="mutY"
                     /locus_tag="PLS16_07050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNJ60875.1"
                     /translation="MTNFNKKLIEWYSDHKRALPWKENVDPYQIWLSEIILQQTRVEQ
                     GLPYYLKFISQYPTIIDFANADITEILKLWEGLGYYSRARNMYVTAQNIRDNFGGKFP
                     NSYHDLRTLKGIGDYTAAAIASFAFGLPNAVVDGNVYRFLARYFGINIPIDTTIGKKY
                     FQEKAQSLINKKKPGEHNQGMMDFGALVCTPKKPKCKDCPFVSECVAYHSNTIEQLPV
                     KSKKMIKKERHLHFFVLIEKNKIIIQQRTKNDIWKLLYDFPNIETESNLLGNEYLKLF
                     SIKTKMPEPIVFKQLLTHQKINAYFYKLNSFKNFTSGVNAIEIDISEIKNYAFPKIIL
                     DYLKCEELYLS"
     gene            2636..2929
                     /locus_tag="PLS16_07055"
     CDS             2636..2929
                     /locus_tag="PLS16_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNJ60876.1"
                     /translation="MRKADIISTIAEKTGVPKVDVIITVESFIKEVKDSLAQGENLYI
                     RGFGSFIRKTRKAKIGRNIKKNTAIEIPEHDIPAFKPSKQFLDQIKNNSIKKK"
     gene            2933..3754
                     /locus_tag="PLS16_07060"
     CDS             2933..3754
                     /locus_tag="PLS16_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ60877.1"
                     /translation="MGKAQWILVSVAILVGASLYFLGRTTTKEKEASVNATTVAKTTT
                     IDFKAIEATFIQKNEVPDTLKIILEQYKNNPKDTNVLLDLALAYRSMHQAHLAGHYFY
                     ELAEVQNNPIYYERAGNAFLNAQRENIDTSISDNLLTFAVQSLEKAIAIEPNSVSAKM
                     LLGTAYVESGLEPMKGVGLLKEVADADPNNLEAQILLGRFSLQSGQYDKAKERLDAVL
                     KKEPNNTEAIYFLAFTEAELGNKKRALELIELCKSLVNNPDFDKEIEHFVKELKK"
     gene            3881..5425
                     /locus_tag="PLS16_07065"
     CDS             3881..5425
                     /locus_tag="PLS16_07065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HNJ60878.1"
                     /translation="MIRELVLHTNSKGVEIALLENKKLVEYHLDVNENDGCAAGNIYL
                     GKIKKLNPALNAAFVDIGHDKDAFVHYSDLSPYIKSIKAFSLNARNTAGATSLDNFIN
                     EPLIDKDGKIEEIFSKGDLLIFEVAKEAISTKGPRLTCEISFPGRYIVLTPFNTSIGI
                     SKKISDEQERQRLTNICEKYKPTGFGIIVRTNAQDISEEELAKNIDLLHKRWIKMSNN
                     MYAQQKPLRLHKEMSKSISIVRDLLNDSFERIITDDEQMLGELKTYIEENIPDKTQLL
                     NLHQSHSPIFEEYDISRQVKTAFGKIVTLKSGAYLIIEHTEALHVVDVNSGPKIKKDI
                     DQDTNAFNINSEAAQEVARQLRLRNIGGIIVIDFIDMKSASYKAKILEIMQEAMKTDK
                     AKHVILPISKFGLMEITRQRVKEQVTIDVAEDIVSNHAKVEEPLQIIDLIYNDLDRLN
                     NHQSKSFLLYVHPFIYAFLKKGTLSFRMKWFSKTNKWIKLVQDSSLRLAEYKLLIQDS
                     GEKAFK"
     gene            5439..6041
                     /locus_tag="PLS16_07070"
     CDS             5439..6041
                     /locus_tag="PLS16_07070"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF011369.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HNJ60879.1"
                     /translation="MNKKTLALLIISILILLDQASKIYIKLTMYMGEHFFYLGDWASI
                     HFVENEGMAFGLSFGGGIGKILLTTFRIVVVSFIAYYIFHQIKQNKANSFFIICLSII
                     FAGAIGNIIDSIFYGVIFNESTPMQIATIFPEQGYAPLLKGKVVDMFYFPLYQGNLPE
                     WVPIFGGKAVDFFPYIFNLADAYITVGVALLLIFQKKILH"
     gene            complement(6056..6871)
                     /gene="murI"
                     /locus_tag="PLS16_07075"
     CDS             complement(6056..6871)
                     /gene="murI"
                     /locus_tag="PLS16_07075"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNJ60880.1"
                     /translation="MDSNTSIGIFDSGIGGLTVANAILHELPNERIIYFGDTAHLPYG
                     DKSASAIQYYAKNITNFLSSKNCKVIVIACNTASAVAYDLLQKIVPKDVFVINVIDPV
                     VEYIINKGYKKVGVIGTIGTIGSNIYKIKIKEQNAKIKVKQLATPLLASMIETGFING
                     QISQLIIEDYLLKPEIQDIDTLVLGCTHYPLIKKEIEDYYKQQNLQVDVLATNEIVGA
                     YVKQYMISEGIIRYEKSEEEHQFFVSDYTETFAQTTRMFYGKDIKLEEARIFD"
     gene            complement(6887..7432)
                     /locus_tag="PLS16_07080"
     CDS             complement(6887..7432)
                     /locus_tag="PLS16_07080"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNJ60881.1"
                     /translation="MRKLFLLFALVTLFAGTTLAENKFGYMNSMEIISLMPEAQAADS
                     AIQKYAVELDGLINKMYTEYDAKAADASSKKQKGLLTPEQEELIVKDLQDLEKRITDF
                     QEAADAKIDKKRQALFDPILKKLNDAISTVAKNNGYTYIFDTSSQALLYAIESDNVLP
                     LVKNALNIKDTPAPKTSTTPK"
     gene            complement(7487..7999)
                     /locus_tag="PLS16_07085"
     CDS             complement(7487..7999)
                     /locus_tag="PLS16_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNJ60882.1"
                     /translation="MKKVFLLFGLFVMVASSAMAQRFAYIDTKYILDKLPEYTKAQSL
                     LDNQAAQWQKEIDTKSEQLDKMYSKYDAESFLLTNDLKKQREDEIIKAENEIQDLQKK
                     RFGFEGDLFTKRKELIQPIQDKVFDAVQKIAEQRAYDFIFDKASGGATMLYTNPKYDV
                     SEEVIKKLGL"
     gene            complement(8010..10685)
                     /locus_tag="PLS16_07090"
     CDS             complement(8010..10685)
                     /locus_tag="PLS16_07090"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HNJ60883.1"
                     /translation="MGHKITNILFSLFFFFIIIPNAVAQEEASDNTFILDYKKPKSFK
                     IESVDIIGTNFLDKNIIRSLSGLKVGDNITVPGDDIAKAIKNLWKQGLFGDVEIQASK
                     IEGDKIYLDIIVVEKPRLNTITVQGVKKGEKDELTKMLDGLRAKPMTDALNANIKNII
                     ADYLEGKSYLHPIIDVVQIKDSTLVNSASVIINIDKGPKALINSVVVDGRENIELSKV
                     RKAMKNTREQTKIDLGYNAHPEYSRKQKIKNTLYTLGNLNLTSIKDFFAERFRISFKG
                     TKFNEDKYEEDKVSLINYYNTVGYRDAKIVYDTVVVKPTGVVDVKIKVEEGNKYYFGN
                     ITFKGNTKYTSDVLSTQLGIKKGDVYNAELLQKRINGDMENGDISSLYYDDGYLFFNI
                     NPIETAVYNDTIDIELRVNEGSQATIKNIIIDGNDKTNEHVIRRELRTYPGDKFSRSN
                     LIRSQRELANLGFIDPQETQVVPIPNPTDGTVDIHYTVKEKSADQIELSAGWGGQQGG
                     LIGTLGLKFNNFSLRNMFKKGAWSPLPTGDGQQFSVRAETNGKRYQNYSFSFTEPWLG
                     GKKPNAFTFSAFRSRLQDVLNKEVLGKQVTNGLSVALGTRLKKPDDYFVFQASADYYG
                     YQLQNFGGRYFVNGKALTDGKFNNLNFSLSLSRNSINNPTFPTSGSNISASVQFTPPY
                     SLFRGKNFDYNGISDSEKYKWLEYHKWRVNFEWFTPLSKSAKKPLVLRLGAKFGFLGT
                     YNKKIGVTPFERFQMGGNGIPSTITLFGTTILAQRGYEGPYSTDGGDPIFTKFLLELR
                     YPFSLNPTATVYGLIFAEAANTYKSISDYNPFKLKKSVGVGIRAILPMFGLIGIDYGI
                     RFDNANGSNLTKASGLFDYIGKNGNITFILGFEPE"
     gene            complement(10701..11465)
                     /locus_tag="PLS16_07095"
     CDS             complement(10701..11465)
                     /locus_tag="PLS16_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNJ60884.1"
                     /translation="MKAEAKNIIYKELVDKDRLPEHIAIIMDGNGRWAKQKGKLRVFG
                     HKNGVQAVREVSEACAEIGVKYLTLYAFSTENWNRPQLEVSALMELLALTIKREIKTL
                     QKNNIRLNAIGNIDSLPEKSREALKETIEATKQNTRMTLTLALSYGSHEEITNAVKNI
                     ALDCLADKLKPNDINPELIGQYLYTKNMPNPDLMIRTSGEHRVSNFLLWQLAYAEFYF
                     TPVLWPDYNKECIFQAIVEYQQRERRFGKTTEQIHQ"
     gene            complement(11465..12280)
                     /locus_tag="PLS16_07100"
     CDS             complement(11465..12280)
                     /locus_tag="PLS16_07100"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HNJ60885.1"
                     /translation="MLGVFCKFNVQAQEKEFGAWAGVAVYLGDLNPNMSFKNARIGTG
                     LFFRYNINNRMSFRAGVNYAYLQAKDSYLKRYPYLLHRNLDFSSQIIEVAGNYEINFF
                     AYSPVGGRNVKTWTPYIFAGGSIFYNNPVSEYNGKKYKLEYVGTEGQKSTNVQSRTKG
                     YNSYAFAIPYGFGIKYALTQNWALNFEVSSRFVFSDFLDDVSGVYPDISHINYYENGV
                     NIGEFIYDKSTEVGPKIGVTNKQRGTSSDKDRFLFVGVGITYTIFETKCPKVF"
     gene            complement(12530..12754)
                     /locus_tag="PLS16_07105"
     CDS             complement(12530..12754)
                     /locus_tag="PLS16_07105"
                     /inference="COORDINATES: protein motif:HMM:NF024763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HNJ60886.1"
                     /translation="MITIIQQRAKCIGCNYCVELAPQRWRMSKKDGKSVLLGAIDKKG
                     FWSVKVREDELDSNIAAAKACPVNIIQVIE"
     gene            complement(12751..13995)
                     /locus_tag="PLS16_07110"
     CDS             complement(12751..13995)
                     /locus_tag="PLS16_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HNJ60887.1"
                     /translation="MGKKFELMAPAGSYEALMAAIHAGCDSVYFGVEQFNMRARATMN
                     FTLEDLPKVCTICKENNVRSYLTLNTIVYDHDLSAIKTVLDAAKSAGATAVIAADQAV
                     IAYARSINLEVHISTQVNVTNIETVKFYALFADTIVLSRELSLIQVKKITDIIEKEQI
                     KGPSGRLIEIEIFGHGALCMAVSGKCYLSLHTANASANRGACVQNCRRSYKVIDNEDG
                     HELEIDNEYIMSAQDLCTIDFLDQVLDAGVTVLKIEGRGKGADYVKTTVECYREAIDS
                     YQAGTYTKEKVAEWMERLKSVYNRGFWSGYYLGQKLGEWNDAPGSKATTKKIFLGKGV
                     HYFDKIKVAEFKLESYELSVGDKILITGPTTGAIETTIEELHNDKGAVNTAKKGESCA
                     FKLDTPIRTSDKLYKIVDATLV"
BASE COUNT         4744 a         2394 c         1943 g         4960 t
ORIGIN      
        1 ttccaaaaca tcttcgaggg taacaattcc gagcgtaccg ccatattcat ctacaatgac
       61 tgccatgtga atccttttgt tttgaaaatc ttctaataag tcatctattt tcttgctaac
      121 tggaacaaaa aaggctggtt ttatcaattt ttgccaatca aaactatctg tttcttccaa
      181 atatagtaat aaatctttcg cataaataat tcctgaaatt ttatcgatac tatcttcata
      241 aactggaatt cttgaatatc cagagtcttt aaccattgcc aaaagttggt ttagtttaat
      301 atttttatcc actgccacca catctatacg tgaacgcata atttgagaaa cagtaatatt
      361 tccaaatttt atcaagcctt ttaaaatttg tgaatctttc tttttatgtt catcagcagt
      421 cgtattgtca attgcttcat caatatcctc ggcagtcaaa ctattggaag tattttccat
      481 ttgtgtctca atcaattcgg caatacttat aaataatttt gtaaatggtt taaaaagaat
      541 atttaaaaac caaactaaat aaatcaatct ttgactccat tttaaattat gttcctttcc
      601 ataaattcta ggaattattt ctacaaaaaa caataagcac aaggtaagta taagcaatct
      661 taaaaacaga taccatcctc tattttctac aacattaagt gaataaaacc aagtattaaa
      721 aagattttct gtaagaatga ctattccaaa actaaagaaa gtaattgcat ataaataagt
      781 tgctaaaagc tgtttcggtt tatgaataaa ttgctctaaa attttatact ttttactctg
      841 ttgtttttta atttgagcta tttgatattg attcaatgaa aaaactgcat acttagaaat
      901 actaacaaac atcgcaagaa acaacaatat tagaattcca aagatttgaa aataaaaggt
      961 aggtaaaact acaaagctaa gaaaaggaat taccgagaat gtctgtatgg gtatgtcgtc
     1021 gctcaaaact tatcaattaa attaaaaagg caaatcatca ttctgaatac cttgctccaa
     1081 atcagtgtct ggtctgtcta aatgttctcc tggttgcgta tctgcattag caacattgcc
     1141 atcttgtttt ttctccaaaa gttgaaaatt ttccaccaca atctccgttg agtatctttt
     1201 attgccttct ttatcgtccc aagaacgtgt ttttatttta ccttcgataa aaacattatt
     1261 tccttttttt aaaattcttt cagcacgttc agccacacca cgccacatta caatattatg
     1321 ccattcggtt tgttctaaat aagttccatc ttccttttta taaaattctt ttgtagctag
     1381 cggaaaatta caaactgttg catttgtgct taatttacga acttctgggt cttttcccaa
     1441 tctccctatt agaattactt tattgacact catcttaatt ttcgtttagt tatggcttaa
     1501 tatacatcta agataaatat aattcttcac atttcaaata atctaaaatt atttttggga
     1561 aagcatagtt ttttatttcg gaaatatcta tttcaatagc atttactcca cttgtaaaat
     1621 ttttaaaaga attgagttta taaaaatagg catttatttt ttggtgtgtc aataattgtt
     1681 taaatacaat aggttcaggc attttagttt tgatagaaaa caactttaaa tattcatttc
     1741 ctaacaaatt actttcagtt tcaatatttg gaaaatcgta caataatttc caaatatcat
     1801 ttttggttcg ctgttggata ataattttat ttttttcaat aagcacaaaa aaatgtaagt
     1861 gcctttcttt tttaatcatt tttttagatt tcactggcaa ttgttcaatt gtattagaat
     1921 gatatgctac acattctgat acaaaaggac aatctttaca ttttggcttt tttggagtac
     1981 aaactaatgc tccaaaatcc atcattcctt gattatgttc tcctggcttt tttttattaa
     2041 ttaaagattg tgctttttct tgaaaatatt tttttccaat tgttgtatca atcggaatat
     2101 taattccaaa atatctggct aaaaatcgat aaacattacc atcgactacc gcattcggca
     2161 atccaaatgc aaatgaagca atagctgccg ctgtataatc tccaatacct tttaatgttc
     2221 tcaaatcatg atacgaatta ggaaatttgc ctccaaaatt atctctaata ttttgagcag
     2281 taacatacat attccttgct cttgaataat accctaatcc ttcccataat tttaaaattt
     2341 cagtaatatc tgcatttgca aaatcaataa ttgttggata ttgactgata aatttcaaat
     2401 aatagggcaa tccttgttcc actcgagttt gttgaagaat aatttcggaa agccaaattt
     2461 gatatgggtc aacgttttcc ttccaaggca atgctctctt atggtctgaa taccactcta
     2521 ttagcttttt gttaaaattt gtcattcaat ttaatataaa ctaaaccata aataatgtta
     2581 gaaatttctt ttatcagtac aaatttcgca attttgtcgc taatattaat tagttatgag
     2641 aaaagcagat attatttcta caattgctga aaaaacaggt gtaccaaaag tagatgttat
     2701 tattacagtt gaatctttta tcaaagaagt taaagattct ttagcacaag gtgaaaatct
     2761 ttatatcaga ggatttggta gttttattag aaaaacaaga aaagctaaaa ttggtagaaa
     2821 tatcaaaaaa aatacagcaa ttgaaatccc tgaacacgat attccagcat tcaaaccttc
     2881 taagcaattc ttagatcaaa ttaagaataa tagcattaaa aagaaataag ctttgggaaa
     2941 agcacaatgg atattagtat cagttgctat tcttgttggt gcaagtctat attttttagg
     3001 tagaacaacc actaaagaaa aagaagcaag tgtaaatgca actactgttg caaagacaac
     3061 aactattgat tttaaagcaa tagaagcaac cttcatacaa aaaaatgaag ttccagatac
     3121 tttaaaaatt attttagaac aatacaaaaa taatccaaaa gataccaatg tactacttga
     3181 tttagcatta gcttatagaa gtatgcatca agcacatttg gcaggacatt atttttatga
     3241 attggcagaa gttcaaaata atccaattta ttatgaaaga gctggaaatg catttctaaa
     3301 tgcacaacga gaaaatattg atacaagtat ttcagataat ttgcttacct ttgcagtcca
     3361 atctttggaa aaagccatag ctatagagcc aaatagtgtt tctgcaaaaa tgttattagg
     3421 tacagcttat gtagaaagtg gcttagaacc aatgaaagga gtaggattat tgaaagaagt
     3481 agcagatgca gatcctaata atttggaagc tcaaatttta ttaggtagat tttcactaca
     3541 atcaggtcaa tatgacaaag ccaaagaaag attggacgct gttttgaaaa aagagccaaa
     3601 taataccgaa gctatttact ttttagcatt tacggaggca gaattaggca acaaaaagcg
     3661 tgcattagaa ttgatagaat tatgtaaaag tttggtcaat aatccagact ttgacaaaga
     3721 aatagaacat tttgttaaag aattaaaaaa ataaaaatta tgccttcagg aaagaaaaga
     3781 aaaagacata agatttctac gcacaagaga aagaaaagac ttagaaaaaa cagacataag
     3841 aagaaaaaat aatacctaat tattcactaa aaattatgtg atgataagag aattagtatt
     3901 acatactaat tccaaaggtg ttgaaattgc gcttctagaa aataagaaat tagtcgaata
     3961 tcatttagat gtcaatgaaa atgatggctg tgctgctgga aatatctatt taggtaaaat
     4021 taaaaagtta aatcctgcat taaatgctgc atttgtggac atcggacacg ataaagacgc
     4081 atttgtacat tactcagatt taagtccata tattaaaagc ataaaagcat tttcgcttaa
     4141 tgctcggaac acagcaggtg caacatcttt ggataatttt atcaatgaac cactcattga
     4201 taaagatggt aaaattgaag agatctttag taaaggtgat ttattgatat ttgaagttgc
     4261 taaagaagcc atctcaacaa aagggccaag actaacttgt gaaatttcat ttccaggaag
     4321 atatattgta cttacgccat tcaatacttc aatcggtata agcaaaaaaa ttagcgacga
     4381 gcaagaacga caacgcctta caaatatttg tgaaaaatat aaaccaactg gttttggtat
     4441 cattgttcgc acgaatgctc aagatatttc agaagaagaa cttgcaaaaa atatcgatct
     4501 actacataag cgttggataa aaatgtccaa caatatgtat gctcaacaaa aacctttgag
     4561 attacataaa gaaatgagta aaagcatttc tattgttaga gatttactga acgatagttt
     4621 tgaacgaatt attaccgatg atgagcaaat gttaggcgag ttaaaaactt atattgagga
     4681 aaatattcct gataaaacac agttgttaaa cctccaccaa tctcactcac cgatttttga
     4741 agaatatgat ataagcagac aagtgaaaac agcattcgga aaaatagtta ccctaaaaag
     4801 cggagcatat ctgattatag aacacacgga agcattacac gttgttgatg tgaatagtgg
     4861 tccaaagata aaaaaggata ttgaccaaga tactaatgca tttaatataa attcagaagc
     4921 agcacaagaa gttgcaagac agctaagact ccgaaatatt ggtggcatta tcgttattga
     4981 ttttatagat atgaaatctg cttcgtataa agctaagata ttggaaatca tgcaagaagc
     5041 gatgaaaaca gacaaagcga agcatgttat tttaccaatt agcaaatttg gattaatgga
     5101 aattacacga caacgtgtta aagaacaagt taccattgat gttgcagaag atattgtaag
     5161 caatcatgca aaagttgaag aacctctcca aattatagat ttaatttata atgatttaga
     5221 tagattgaat aatcatcaat ccaaaagttt cttgctttat gtacatcctt ttatttatgc
     5281 atttttgaaa aaaggaacac tttcatttag aatgaaatgg tttagcaaaa ccaataaatg
     5341 gataaagttg gtacaagata gtagtttgcg attagctgaa tataaattat tgatacaaga
     5401 ttcaggtgaa aaagcattta aatgattaaa ttagtccaat gaataaaaag acattagcct
     5461 tattaataat ctctatccta attttattag atcaagcaag taagatatac attaagctga
     5521 cgatgtatat gggtgagcat ttcttttatt taggcgattg ggcaagtatt cattttgttg
     5581 aaaatgaagg tatggctttt ggtttgagct ttggtggtgg cattggaaaa atacttttaa
     5641 ctacttttag aatcgttgtt gttagcttta ttgcctatta tattttccat cagattaagc
     5701 aaaataaggc aaatagtttt ttcattattt gtttatccat aatattcgct ggagctattg
     5761 gaaatattat agatagtatt ttttatggtg taatatttaa tgaaagtaca cccatgcaaa
     5821 ttgctactat ctttccagag caaggttatg ctccattatt aaaaggaaaa gtagttgata
     5881 tgttttactt tccactttat caaggaaatt tacctgagtg ggttccaatt tttggtggta
     5941 aagccgtaga tttttttcct tatatattta atttagctga cgcttatatt acagtcggtg
     6001 ttgctttatt acttatcttc caaaagaaga tattgcatta aatagattat acttcttaat
     6061 caaaaattcg agcttcttct agtttaatat ctttgccata aaacattctt gtcgtttgtg
     6121 cgaaagtttc agtatagtct gaaacaaaaa actgatgttc ttcttcagat ttttcataac
     6181 gaataatacc ttcagaaatc atatactgtt ttacgtaagc accgacaatt tcattagttg
     6241 ctagaacatc aacttgaaga ttttgctgct tataataatc ttcaatttcc tttttaatta
     6301 aaggataatg cgtacatcct aatacaagtg tatcaatatc ttgaatttca ggtttaagca
     6361 ggtagtcttc aataatcaat tgactgattt gcccatttat aaatccagtt tcaatcattg
     6421 aagccaatag cggagtcgct aattgcttaa ccttaatttt agcattttgt tctttgattt
     6481 tgattttata gatattagaa ccaatagttc caatagttcc aatcacacca acttttttat
     6541 agcctttgtt gataatgtat tcaacaactg ggtcgattac attaattaca aatacatctt
     6601 tcgggacaat tttttgtaac aaatcgtaag ctacagctga tgccgtatta caagcaatta
     6661 caataacttt acaattttta gaagataaaa aattagtgat atttttagca taatactgaa
     6721 tagcacttgc tgacttatcg ccatagggca aatgggcagt atcaccaaaa taaattattc
     6781 tttcattggg taattcatgt aaaatagcat tggcgaccgt taagccgcca atacctgagt
     6841 caaaaattcc aattgaagta ttagaatcca tatctcgaaa attttattat tttggtgtag
     6901 tgctagtttt aggagctgga gtatctttga tattcaaagc attttttacc aatggcaata
     6961 cattatcact ttctattgca tacaataaag cttgtgaact tgtatcaaag atataagtat
     7021 atccattgtt tttagccact gtagaaattg catcatttaa tttcttcaag attgggtcaa
     7081 ataaagcttg tcttttttta tcaattttag catctgctgc ttcttgaaaa tcagtaattc
     7141 ttttttctaa atcttgtaaa tctttaacga ttaattcttc ttgttctggt gttaataaac
     7201 ccttttgttt tttggaagat gcatctgctg cttttgcatc atactctgtg tacattttat
     7261 tgattaagcc atctaattct accgcatatt tctgaattgc agaatctgct gcttgagctt
     7321 ctggcatcaa actaataatt tccatagagt tcatatagcc aaatttattt tcagctaaag
     7381 ttgtacctgc aaataaagtt accaatgcaa ataataaaaa taatttcctc atttctttct
     7441 gttgtttagt tgttgttatt taattgattt atttataatt taatgtttat agtcctaatt
     7501 ttttaatgac ttcttcgctc acatcatatt ttggattagt atataacata gttgctccgc
     7561 cagatgcttt gtcaaagata aaatcgtaag ctctttgttc tgcaattttt tgtacagcat
     7621 caaaaacttt atcttgaatg ggttgaatta attcttttct cttagtgaac aaatcgcctt
     7681 caaacccaaa acgtttcttt tgtaaatctt gtatttcatt ttctgctttt atgatttcat
     7741 cttctctttg ctttttcaaa tcattggtca ataaaaatga ttctgcatca tactttgaat
     7801 acatcttgtc caattgctca gatttggtgt caatttcttt ctgccattgt gctgcttgat
     7861 tgtctaataa tgattgtgct ttagtatatt ctggtaattt atccaaaata tatttggtat
     7921 caatataagc aaatctttgt gccatagcag aactcgctac cataacaaac aatccaaata
     7981 ataaaaatac ttttttcata attaaaattt tattctggtt caaatcctaa tataaatgtg
     8041 atgtttccat ttttacctat atagtcaaat aagccagatg ctttagttaa gttactacca
     8101 ttagcattat caaaacgtat gccataatca attccaatca aaccaaacat tggcaatatt
     8161 gctctaatac caacacccac actctttttc aatttgaatg gattataatc cgaaattgat
     8221 ttgtaagtat ttgctgcttc tgcaaatatt aatccatata ctgttgctgt cggatttaaa
     8281 gagaatggat atcttaattc taataaaaat ttagtaaata ttgggtcgcc accatccgta
     8341 gaatatggtc cttcataacc tctctgtgcc aagatagttg ttccaaataa agtaatcgtt
     8401 gaaggaatac catttccacc catttggaat ctttcaaatg gtgttacgcc aattttttta
     8461 ttgtaagtac ccaagaaacc aaatttagca cctaaacgta ataccaatgg cttttttgca
     8521 cttttagaaa gtggtgtaaa ccattcaaaa ttaactctcc acttgtggta ttctaaccac
     8581 ttatattttt cggaatctga aattccatta taatcaaaat tctttcctct aaataatgaa
     8641 tatggtggtg taaattgtac agacgcagaa atattagaac cagaagttgg gaatgttggg
     8701 ttattaatag aatttcttga caaagataga ctaaaattta agttattaaa tttaccatct
     8761 gtcaatgctt ttccattcac aaaatatcta ccaccaaaat tttgcaactg ataaccataa
     8821 taatctgctg aagcttggaa tacaaaataa tcatctggtt ttttcaatct tgtacctaaa
     8881 gcaactgaca aaccattggt tacttgctta cctaatactt ctttatttaa aacatcttgc
     8941 aaacgagaac ggaatgcaga gaatgtaaat gcatttggct ttttgccacc taaccaaggt
     9001 tctgtaaatg agaaactata attttgataa cgctttccat ttgtttctgc acgcactgag
     9061 aattgttggc catctccagt tggcaatggc gaccaagctc ctttcttaaa catatttctc
     9121 aatgaaaaat tattaaactt caaacctaaa gtacctatta agccaccttg ttgtccaccc
     9181 caacctgctg aaagttcaat ttggtctgca gatttttctt tcaccgtata atgaatatct
     9241 acagttccat cagttggatt tggaattggc actacctgag tctcttgtgg gtcaatgaag
     9301 cctaagtttg ctaattctct ttgtgaacgt atcaggttag aacgactgaa tttatcacct
     9361 ggataagttc ttaattctct tcggataacg tgttcattcg ttttatcatt tccatcaata
     9421 atgatattct taatcgttgc ttgagaacct tcgtttactc tcaattctat atctatagtg
     9481 tcattataaa ccgcagtttc aattgggttg atattaaaaa agagataacc atcatcataa
     9541 tacaatgaac taatatcgcc attttccata tcgccattga tacgtttttg taataattct
     9601 gcgttatata catcgccttt tttgatacct aattgtgtac ttaacacatc tgaagtatat
     9661 ttagtattgc ctttaaaagt aatatttcca aaataatatt tattgccttc ctccacttta
     9721 attttgacat ctacaacacc tgttggttta acaacgactg tatcataaac tattttggca
     9781 tcacgatatc cgactgtatt ataataattg ataagactta ctttatcttc ttcatattta
     9841 tcttcattaa acttggtgcc tttaaaagaa attctaaatc gttcagcaaa gaaatctttt
     9901 atagaagtca aattcaaatt tccgagtgta tataaagtgt ttttgatttt ttgttttctt
     9961 gaatattcgg ggtgtgcatt atatcctaaa tcaattttag tttgctcacg agtatttttc
    10021 attgcctttc ttactttaga caattcaata ttctcacgtc catcaaccac tacagaatta
    10081 attaatgctt tagggccttt atcaatatta ataatcactg aagcactatt gactaaagtg
    10141 gagtctttaa tttgtacgac atcaataatt ggatgtaaat aagattttcc ttctaaataa
    10201 tctgcaataa tgtttttaat attagcgtta agtgcatctg tcattggttt tgctcttaat
    10261 ccatctaaca ttttagtcag ttcatctttt tctccttttt tgacaccttg aactgtaata
    10321 gtatttaatc ttggtttttc tacaacaatg atatccaaat aaattttatc tccttcaatt
    10381 ttagaagcct gaatttctac atcaccaaat aatccttgtt tccatagatt tttaattgct
    10441 tttgcaatat catcaccagg cactgtaata ttatctccta ctttcaatcc acttaaagaa
    10501 cgaataatat ttttatctaa aaaatttgtt ccaatgatat ctacagattc tatcttaaaa
    10561 cttttaggct ttttataatc taatataaat gtattatcgc tcgcttcttc ttgagcaact
    10621 gcatttggaa taatgataaa aaagaaaaat aagctaaaga gtatgttggt tattttatga
    10681 cccatttata tttgaaatta ttattgatga atttgttctg ttgttttacc gaatcttctt
    10741 tctcgttgtt gatactctac aatcgcttga aaaatacatt ctttattata atctggccaa
    10801 agaactggcg tgaaataaaa ttcggcataa gctaattgcc aaagtaaaaa attactcact
    10861 ctatgttctc cacttgttct aatcattaaa tctggatttg gcatattctt ggtatataag
    10921 tactgcccaa ttagttctgg attgatatca tttggcttta atttatcagc caaacagtct
    10981 aaagcgatat tttttacagc gttcgtaatc tcttcatgtg aaccataact caatgctaaa
    11041 gttaatgtca ttcgagtatt ttgtttggtc gcttcaattg tttcttttaa agcttcacga
    11101 cttttttcag gtaaagaatc aatatttcct attgcattca gacgaatatt gtttttttgc
    11161 aaagtcttaa tttctctttt aatcgtcaat gccaataatt ccattaaagc agaaacttca
    11221 agctgaggtc ggttccaatt ttcagtagag aatgcataaa gagtcaagta tttaacacca
    11281 atttcagcac aagcttcaga tacttcacgc acagcttgaa cgccattctt atgcccaaac
    11341 acacgcaatt tccctttttg tttcgcccat cttccgttgc catccataat aatagcgata
    11401 tgctctggca atctgtcctt atctactaat tctttataaa taatattttt agcttctgct
    11461 ttcattaaaa aactttaggg cattttgttt caaaaatagt ataagtaatg ccaactccaa
    11521 caaatagaaa tctgtcttta tcacttgaag tgcctctttg cttatttgta acaccaattt
    11581 ttggtcctac ttcagttgat ttatcataaa taaattctcc aatatttacg ccattttcat
    11641 aataattaat atgactaata tctggataaa caccacttac atcatctaag aaatcagaga
    11701 aaacaaaacg gctacttact tcaaaattca atgcccaatt ttgcgtaagt gcatatttta
    11761 taccaaaccc ataaggaatt gcaaacgcat agctattata accttttgtt ctgctctgca
    11821 cattcgtaga tttttgtcct tcggtgccaa catattccaa tttatatttt ttaccattat
    11881 attccgaaac tggattatta taaaaaatac ttccaccagc aaagatataa ggtgtccatg
    11941 tcttcacatt tcgaccacct acaggagaat aagcaaagaa attaatttca taatttccag
    12001 caacttcaat aatttgagag gaaaaatcaa ggtttcgatg tagcaaataa gggtaacgct
    12061 ttagataact atctttagct tgcaaataag cataattcac accagcccta aaagacattc
    12121 tattattaat attatatcta aaaaacaaac cagtgcctat tcttgcattc ttaaaagaca
    12181 tatttggatt caaatctccg agataaaccg caacacctgc ccaagctcca aattcttttt
    12241 cttgtgcttg tacattaaat ttgcagaata ctcccagcat aattagacaa aatattttca
    12301 atcctttttt cagaaaaagc taattttcca acaaagataa gattttcagt caatttttga
    12361 tataaaacca atgcaaaacc cattattttt taggttttaa aattatttaa caaacaataa
    12421 ttgaacaaat tatgtggaaa taaatagttc gtcgtatata atatagactt ctaaaacaaa
    12481 aatctatttt taaatgaaat taaaataccc taaagttcca aaagatatat tattctatca
    12541 cttgaattat atttactgga caagctttgg cggctgcaat attactatct aattcgtcct
    12601 cacgaacctt aacactccaa aatccttttt tatcaatagc acccaacaac acacttttac
    12661 catctttttt actcattcgc caacgttgtg gagctaattc tacacagtaa ttacagccaa
    12721 tacattttgc tctttgttgg ataattgtta tcatactaag gttgcatcaa caattttata
    12781 taatttatcg cttgttctaa tcggcgtatc taatttgaaa gcacaggact ctcctttttt
    12841 tgctgtattt actgcacctt tatcattgtg taactcttcg atggttgttt caatggcacc
    12901 tgtagttggt ccagttatta atattttatc gccaacactt aattcgtaag attctaactt
    12961 aaactctgcc accttaattt tatcaaaata atgaacacct ttacccaaaa atattttctt
    13021 cgtagttgct tttgaaccag gcgcatcatt ccactcgccc aatttctgac ctaagtaata
    13081 accactccaa aaacctcgat tataaacact tttcaaacgc tccatccatt cagcaacttt
    13141 ttctttagta taagttcctg cttgatagct atcaatcgct tctcgatagc actccacagt
    13201 tgtttttaca taatcagcac cttttccccg accttcaatt tttaagacag taacaccagc
    13261 atctaaaact tggtctaaga agtctattgt acataagtct tgtgcagaca tgatatattc
    13321 attatctatt tctaattcat gtccatcttc attatcgatg actttataac ttcttctaca
    13381 attttgtaca caagcacctc gattggctga tgcatttgca gtatgcaaac ttaaataaca
    13441 tttgcctgat actgccatac acaaggcccc atgtccaaaa atctctatct caatcaatct
    13501 acctgatggc cctttaattt gttctttctc aattatatcc gtaatttttt tgacttgaat
    13561 taagctcaac tctcttgaaa gcacaatagt atcagcaaat aatgcataga attttacagt
    13621 ttcgatattc gtaacattaa cttgagtaga aatgtgtact tctaaattga tacttctagc
    13681 ataagcaatt actgcttggt ctgccgcaat cacagcagtt gctccagcac tttttgcggc
    13741 atctaatact gttttaatag ccgacaaatc atgatcataa acgatagtgt ttaacgttaa
    13801 atagctacga acattatttt ctttacaaat tgtacatact tttggcaaat cctctaaagt
    13861 aaagttcata gttgcacgag cacgcatatt gaattgctca acaccaaaat acactgaatc
    13921 acaaccagcg tggatagctg ccattaaagc ctcatagctt ccagctggcg ccattagttc
    13981 aaatttcttt cccataaaat aaagcagtct tcaacactgc ggtacaaaat tagggaataa
    14041 a
//
LOCUS       DAOQCW010000065        13988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1611_length_13988_cov_5.238786, whole genome shotgun sequence.
ACCESSION   DAOQCW010000065 DAOQCW010000000
VERSION     DAOQCW010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13988
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1611_length_13988_cov_5.238786"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2434)
                     /locus_tag="PLS16_07115"
     CDS             complement(<1..2434)
                     /locus_tag="PLS16_07115"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HNJ60888.1"
                     /translation="MRNIYILILMTLVFFNTIAKEHTNNEPNALVKCGTPVPSLKWEM
                     NFQKHIQNYEWNKKNNPNAKNAEESIVIPVIVHVIYDKDTVIENITKAQIDSQFVILN
                     RNYAGNSAYKNNIPSAFLPRVANTNISFCPALVDPSGNVLLEPGINRISRTSVGFSSP
                     GSKGWTRTYIDNTIKPNTIWNPRYYLNIWVVPKIRTEDGDVLLGYATFPESMYASGED
                     GDVDNSKTDGVVCAARGFGKSSTNYTEYNDGGTATHEIGHWLGLRHIWGDENCGNDFC
                     NDTPLHEDANYGCPEFPHITSCGNSPNGEMFMNYMDYTDDYCTNMFTLDQKVRMMTSM
                     NTDSLRNALLSSPVCNSTMQEPLANFNYALTSTDICGTQKYNFFDKSMWNKNATSWTW
                     TFQDATPTTSSVKNPTDITFNSSGEKQVTLTIVTPLGTSTVTKKIIVAMVGNSTLPLL
                     ENFEGSVFPPNGWVLKKRSVEPNLNWELRKGYSAFGLGNQSMIFNNTEYDGKKKIDDI
                     LSPKINLTGVTQAKIKFDVAYAPYYVINSDNSIDIQYDKLEVHLTTNCGTTSTSVYKK
                     EGTALATYLPGIGEEFYPTASQWRTDSIDIPASFLNKADVQLVFRNYGMYGHTIYIDN
                     INVYNTQPAAVVLNPNFTMSKSEICIGESITLNNTTPELIDSLRWNISGGNPFTSNNS
                     NLSVQYGSSGNYNIQLTVFKNNSSSTITKTIKVNSKPTVSISPANTTICVGQSVTLIA
                     SGASSYAWSTGQSGNTIVVNPSVNSTYKVLGIANNCTSDSAIAIVNITTTNPTVSITK
                     SPNVSTICEGDSV"
     gene            complement(2558..2968)
                     /locus_tag="PLS16_07120"
     CDS             complement(2558..2968)
                     /locus_tag="PLS16_07120"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNJ60889.1"
                     /translation="MKKLYFILSFVFAGFFGFAQTDANAPVVDANAPQITFKTESIDY
                     GTIKQDASGLRVFEFTNTGKSPLIITSATGSCGCTVPTYPKEPIMPGQKAEIEVRYDT
                     HRIGAFSKTVTVNTNASTPTKTLRISGTVEAVAP"
     gene            complement(3060..3599)
                     /locus_tag="PLS16_07125"
     CDS             complement(3060..3599)
                     /locus_tag="PLS16_07125"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNJ60890.1"
                     /translation="MKNEILADLNKVEVLYNQYLEDTETMTDAQFNQIPNADEWSLAQ
                     ALYHTWTAIDLTVLFINKQMTSAKQRKSITLKNSIRYILLKIALFSPLKFKVPPQLKD
                     KMIAHITHQELKEKSQDTFARFKTLIETFPKELETNEIFFHPVIGWINLQQSVGFLIE
                     HTLHHKAQIYRIKEQVLKS"
     gene            3717..4904
                     /locus_tag="PLS16_07130"
     CDS             3717..4904
                     /locus_tag="PLS16_07130"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNJ60891.1"
                     /translation="MFLDFFLLLREEGLPVTITEYLHLLEALNEKVVEYSVDDFYYLS
                     RTTLVKNEQFLDRYDILFAQYFNGVQLEDLSDAKIDEDWIKKEIDRLFSDEEKKMIEK
                     MGGLDKLMERFKELLEKQKERHQGGNTWIGTGGTSPFGNGGYNPEGIRIGGSGGGKSA
                     IKVWEKRDFQNYASDVELNTRNIKMALRRLRILTREGIEDELDLHTTIEKTCKNAGYL
                     DIEMQASKKNKVKILLFFDVGGSMDPYIDICEQLFSAAKTEFKHLEFFYFHNCIYGSV
                     WKNNELRHQDRIPTFEILHKYNSDYRIIFVGDATMSPYEITANGGSVEHFNDESGATW
                     LNRFVEKYKNIVWLNPSNEYFWDDIPSVQIIKQIFKDRMYALTLDGISNAMKTLKKNK
                     RAV"
     gene            complement(4931..6115)
                     /locus_tag="PLS16_07135"
     CDS             complement(4931..6115)
                     /locus_tag="PLS16_07135"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HNJ60892.1"
                     /translation="MRFGLSLILFFLFFGFSKAQVQNFNVPKISSDAKISLLTVGVGE
                     EIYQLFGHTGIRIKDEKLGWDLVYNYGTFDFYDPNFIKKFIKGELDYFLSVDDFTAFM
                     NEYVEENRSVHEQVLDLDSTQVQKIFEFLTTNAREEFKYYRYDFLYDNCATRPRDVIV
                     KVLFKDQLKFKPDVDDEATYRELIDRHNSNEWLDFGMDIAIGLPTDKKAGYGRTFLPF
                     ELQQLIEGATLNGKSIVLSSSQILDTIPEHHSKKWFTPGLLFWLIFLFFFILQIKNKF
                     NAVLYKVVAVTFFTTLGLLGWLFIYFWFGTNHTTTYWNLNILWAMPLNLPIALFILSN
                     KYRKWVHQYFFVYRMILVILLCGWGLNPQQYHAAVVPIILLAILLNSKYLTIPTSKEI
                     DR"
     gene            6212..6988
                     /locus_tag="PLS16_07140"
     CDS             6212..6988
                     /locus_tag="PLS16_07140"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HNJ60893.1"
                     /translation="MKTKEEIVHNWLPRYTGKKLEEFGEYILLTNFEKYIELFAEIHQ
                     VEVDGLNSPMPNATAGNITIINFGMGSAVAATILDLLSAIKPKAALFLGKCGGLKKKN
                     EIGDFVLPIAAIRGDGTSNDYFPPEVPALPSFALQKAISTTIREFQQDYWTGTVYTTN
                     RRVWEHDDTFKEYLRKLRAMAIDMETATIFSVAFANEIPVGALLLVSDQPMIPEGIKT
                     SESDKNVTKNYVDLHLKIGIASLQQLQNKTTTIKHLRFED"
     gene            complement(7040..7771)
                     /locus_tag="PLS16_07145"
     CDS             complement(7040..7771)
                     /locus_tag="PLS16_07145"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNJ60894.1"
                     /translation="MKKYYFILVFVLGFVACNNGNASQDNGTTQSINENLDVNQFAEK
                     VVGNSGIHLLDVRTEEEYASGHLENAQNININGASFADEVAKLDKNKPVYLYCLSGAR
                     SAKAASYLSQQGFKEVYNLKGGIIAWKGAEKPVTQESNANKGLNLADYNKLVSADKMV
                     LVDFYAPWCGPCKILSPIVKEVETEMGTFKLQKINYDEAGEVVSHLKIAAIPLLVLYK
                     EGKEVWKHNGTIEKAELKKIIQQYQ"
     gene            complement(7834..9351)
                     /gene="lepB"
                     /locus_tag="PLS16_07150"
     CDS             complement(7834..9351)
                     /gene="lepB"
                     /locus_tag="PLS16_07150"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNJ60895.1"
                     /translation="MLSIIYFYLLYYFLHFSLYGIFKKAGKNPIHVFIPFLQDATWLE
                     IIGKNKLKAFWGLIPYVNFLFALTWVTDTLNCFGKTSFLHHTFASLFGFISFSYIGFV
                     DKNAKYLGPLYADKNFKHKRNMAREWGDAIVFAVIAATFVRMFTLEAYKIPTTSMEGS
                     LLAGDFLFVSKFNYGARIPQTPIAFPFAHHTMPLGLGKAYSTLIQLPYKRLPGFEKVT
                     KGDVVVFNYPGDAVEHPERPIDKRENYVKRCVGTPGDTLQIINRQLFINNEKQAHYTN
                     MQFLYSVVLKQKLNRPLIDELGLLWFYDFAINYADGYGNPIAPLNQIEEDFDNYISQS
                     DSVLIFLTDNQIELMKQKNLIQTIAEFKQEQPIQFLEDIFIAPSQLGWTVDNFGPLVI
                     PKKDMTIDLTAENFVKYYSTIKYFEKHSIEVNNNRQFIIDGKLATQFTFTQDYYFMMG
                     DNRHNSADSRFWGFVPEDHIVGKPLFVWLSMNNFEQIPKSIKWNRMFKSVTNLSK"
     gene            9490..9954
                     /gene="smpB"
                     /locus_tag="PLS16_07155"
     CDS             9490..9954
                     /gene="smpB"
                     /locus_tag="PLS16_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNJ60896.1"
                     /translation="MAKKATHIELVNRKASFNYELLESYKAGIILAGTEIKSIREGKA
                     NLSDSYCFFKDDELWVKNLHIAEYSFGTYNNHVPLRQRKLLLQKRELKKLHSKIKERG
                     FTIIPYKLFFNERNLCKLEIYLARGKKSFDKRETIKARDEQKNIDRIKKSYR"
     gene            complement(9968..10507)
                     /locus_tag="PLS16_07160"
     CDS             complement(9968..10507)
                     /locus_tag="PLS16_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60897.1"
                     /translation="MSKFPIEEVDKIKYKAKKGIDRMAKKGALMVKLNKNGQEFYVVG
                     THLNAAGSDDYRICQLKQLKTELLDKYEKDSLPTIVAGDLNVDKYKRSLYDSMLGILN
                     VNDYKMEGDLQLTYDYTKNTLAYGKSQNWIDYIFLMQHQLKVEDVKREVKIIESPWSK
                     KHQSLSDHHAVEMKLYYKK"
     gene            complement(10547..10807)
                     /locus_tag="PLS16_07165"
     CDS             complement(10547..10807)
                     /locus_tag="PLS16_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60898.1"
                     /translation="MPINPTQLPEQEVALDSLKMLSWNIYMLPPMLKFTGKKKRSIHI
                     ANQLLTTDYDLIVFQEAFHRGARRRMVKRMQTDYPYRIGPAF"
     gene            11269..12813
                     /locus_tag="PLS16_07170"
     CDS             11269..12813
                     /locus_tag="PLS16_07170"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR00497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HNJ60899.1"
                     /translation="MTSTAQRAELQAKIWKIANEVRGAVDGWDFKQFVLGALFYRFIS
                     ENFSKHFDDADTVYANLADDVISSEIKDDAIKTKGYFIYPSQLFVNIAKSASTNKNLN
                     TELKAIFDAIESSANGYASEDDIKGLFADFDTTSNRLGNSVPDKNKRLAAVLKGVEEL
                     NFGNFENNQIDLFGDAYEFLISNYAANAGKSGGEFFTPQTVSKLIAQLALHKKKKVNK
                     IYDPACGSGSLLLQAKKHFDSHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFHIL
                     LGDTLLKPALGDEKPFDAIVSNPPYSVTWIGSDDPTLINDDRFAPAGVLAPKSKADFA
                     FVLHALSYLSSKGRAAIVCFPGIFYRGGAEQKIRQYLVDNNFVETVISLAPNLFFGTS
                     IAVNILVLSKNKKENSTQFIDASGEDFFKKVTNNNVLEDSHIERIMELFDKKENVAHT
                     AITVENSKIAENDYNLSVSSYVEAKDNREVIDIKVLNAEIAETVKKINALRTDIDKII
                     KEIETV"
     gene            12913..>13988
                     /locus_tag="PLS16_07175"
     CDS             12913..>13988
                     /locus_tag="PLS16_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60900.1"
                     /translation="MPYNPQKHHRRSIRLKGYDYAQAGLYFITICCQDRANLFGAIEN
                     GEMILNEYGQIAFEEWANTENIRDNCRIHEYIIMPNHIHGIIEIIFKKENNMDEALIS
                     NNSKEENLNSSTGEEIISDNKSESIISENTGKEIISDNKSESIISENKSESIISDNTG
                     EEIISDNTGEEIISDNKSESIFFDSTGELQFAPTAPTEFPPTAPKFKSPSQTIGSIIR
                     GFKIATIKRIKDLILKEKDNMSEALISNNSEEKNLNSSTGELQFAPMEFAQTEFAPTA
                     PTEFASTAPIIPNNSEDSIISNNKSKEIISDNKSKSIFSDSTGELQFAPTEFAPTEFA
                     PTAPIITNNSEDSIISNNKSKEIIS"
BASE COUNT         4874 a         2328 c         2067 g         4719 t
ORIGIN      
        1 ttacagaatc gccctcacaa attgtactta cattcggaga ttttgtaata cttacagttg
       61 ggtttgttgt tgtaatgttt actattgcaa tagctgaatc tgatgtacaa ttatttgcaa
      121 ttcctaatac tttataagta gaatttactg aaggatttac gactatagta tttccacttt
      181 gtcctgtact ccaagcataa ctacttgctc cacttgctat aagtgttaca gattgtccaa
      241 cacaaattgt tgtatttgca ggactaatac ttacagtagg ttttgaatta actttaattg
      301 tttttgttat tgtagaagaa ctattatttt taaaaactgt caattgaata ttataatttc
      361 ctgaagaacc atattgtaca gataaatttg aattattcga tgtaaacgga ttacctccac
      421 taatattcca tctcaaagaa tcaattaatt ctggagttgt attattaagt gttattgact
      481 ctccaataca tatttcagat ttagacatcg taaagttagg atttaatacc acagcagctg
      541 gttgcgtatt gtaaacattg atattatcaa tataaatcgt atgtccatac attccataat
      601 ttctaaaaac taactgaaca tctgctttat ttaaaaatga ggctggaata tctatagaat
      661 ctgttctcca ctgactagca gttggataaa actcctcacc tatgcctggt aaataagtag
      721 ccaaagcagt accttccttt ttataaacag atgtagaagt agtaccacaa tttgttgtca
      781 aatgaacttc tagcttatca tattgtatat caatagaatt atccgaattg attacgtaat
      841 atggagcata ggcaacatca aatttaattt ttgcttgtgt aactccagtt aaattaatct
      901 ttggagataa tatatcatct atctttttct ttccatcata ttctgtatta ttaaatatca
      961 ttgattgatt tcctagccca aaagcactat atccttttct taattcccaa tttagatttg
     1021 gttcaacaga acgctttttc agcacccaac cattaggagg aaaaactgag ccttcaaagt
     1081 tttcaagtaa tggaagtgtt gaatttccaa ccattgcaac aataattttt ttcgtaacag
     1141 tagaagttcc taatggcgta acaattgtta atgtaacttg tttttcacca gaagaattaa
     1201 aagtaatatc cgttggattt tttactgaag atgttgtagg agttgcatct tgaaatgtcc
     1261 aagtccaaga tgtcgcattc ttattccaca ttgatttatc aaagaaatta tatttttgag
     1321 taccacaaat atccgtagaa gttaaagcat aattaaaatt agctaatggt tcttgcatcg
     1381 ttgaattaca aacaggagaa ctcaataaag catttctaag actatcggta ttcattgatg
     1441 tcatcatacg cactttctga tctaaagtga acatatttgt acaataatca tctgtataat
     1501 ccatataatt cataaacatt tcaccatttg ggctatttcc acacgaagta atatgtggaa
     1561 actcaggaca accataatta gcatcttcat gtagtggtgt atcattacaa aaatcatttc
     1621 cacaattttc atctccccaa atatgtctca aaccaagcca atgaccaatt tcgtgtgttg
     1681 ctgtacctcc atcattatat tctgtataat tagtagaact cttaccaaaa cctctcgctg
     1741 cacaaactac accatctgtt ttactattat cgacatcgcc atcttctcca gaagcataca
     1801 tactttcagg aaaagtagca taacctaaaa gtacatcacc atcttcagtt ctaatttttg
     1861 gcactaccca aatattcaaa taataccttg gattccaaat tgtatttggc tttatagtat
     1921 tatcaatata tgttctagtc caaccttttg aaccaggaga tgaaaatcca acagaagtac
     1981 gactgattct attaattcct ggttccaata aaacatttcc gcttggatca actaatgctg
     2041 gacaaaacga aatattagta ttcgctacac gaggcaaaaa tgcacttgga atattattct
     2101 tataagcact attccccgca taatttctat ttaaaatgac aaattgagag tcaatctgtg
     2161 ctttagttat attttcaatt actgtatctt tatcatatat aacatgtaca ataacaggaa
     2221 tcacaatact ttcttcagca ttttttgcat ttggattatt tttcttattc cattcataat
     2281 tttgaatatg tttttgaaag ttcatttccc attttaaact aggaactgga gttccacatt
     2341 ttactagtgc attgggttca ttatttgtgt gttcttttgc aattgtatta aaaaatacaa
     2401 gtgtcattaa aattagaata taaatatttc tcataaattg ggtagcaaaa ataattattc
     2461 tattgaatat aaaatattat ctttttcaac attaacattt tgtataaaaa aagcagaacc
     2521 gaagttctgc ttttataatt taacttaact aattcaatta aggtgcaact gcttcaactg
     2581 ttccactaat tcttaatgtt ttagttggag tagatgcatt tgtgtttaca gtaacagttt
     2641 tgctgaaagc tccaattctg tgagtgtcgt atctaacttc aatttctgct ttttgtcctg
     2701 gcataattgg ttctttagga tacgttggca cagtacaacc acaagagcct gtcgcacttg
     2761 taataattaa tggagattta cctgtatttg taaattcaaa cactctcaat ccacttgcgt
     2821 cttgtttaat tgtaccataa tcaattgact cggttttaaa cgtaatttgt ggtgcattcg
     2881 catcaactac tggtgcattc gcatctgttt gtgcaaaacc aaaaaatccg gcaaacacaa
     2941 aacttaagat aaagtatagc tttttcattt tcaaaaaatt taattagtgt ataacaaatt
     3001 taatgccttt ttttagaaat catcaaaaat taagattcca aactcatttt taacatattt
     3061 taggatttca atacttgctc cttaattcta tagatttgtg ctttatgatg taaagtatgt
     3121 tctatcaaaa agcctactga ctgttgtaaa tttatccaac caattactgg atgaaaaaat
     3181 atttcattag tttccaattc tttaggaaag gtttctatta aagttttaaa acgagcaaat
     3241 gtatcttgac tcttctcctt caattcttgg tgtgtaatat gtgctatcat tttatctttt
     3301 aattgtggtg gcactttaaa ttttaaaggg gagaataatg ctatctttaa aagtatatat
     3361 cttatggaat tttttaatgt tattgattta cgttgtttag cagaggtcat ttgtttattg
     3421 ataaaaagta ccgttaaatc tattgctgtc caagtatgat ataacgcttg tgctaaactc
     3481 cattcatcag catttggtat ttgattaaat tgagcatctg tcatagtttc tgtatcttct
     3541 aaatattgat tatacaatac ttctacttta ttcaaatctg ctaatatttc atttttcata
     3601 tttataaaat taattaaaaa aattatctat ttcaacaatc ttagaatatt tagtaatttt
     3661 acacttagat aaagacgtac ttttatctat ctgttttata ctcaatttga agcattatgt
     3721 ttttagattt ttttttactg ctcagagaag aaggtttgcc tgttaccatt acggaatatt
     3781 tgcatttatt agaagcttta aatgaaaagg tagtagaata tagtgtggat gatttctatt
     3841 atttatccag aacaacttta gtaaaaaacg aacaattctt agaccgttac gatattcttt
     3901 ttgctcaata tttcaatggt gttcaattag aagatttatc tgatgccaaa atcgatgaag
     3961 attggattaa aaaagaaata gaccgcctct tttctgatga ggaaaagaaa atgattgaga
     4021 aaatgggtgg tttggataaa ctaatggagc gatttaaaga attattagaa aaacaaaaag
     4081 aaagacatca aggtggaaat acttggattg gaaccggtgg cacttctcct tttggtaatg
     4141 gtggttataa tcctgaaggt ataagaatag gtggcagtgg cggaggaaaa agtgctatta
     4201 aagtttggga aaaacgagat tttcaaaact atgcttcaga tgtagaatta aatactcgta
     4261 atataaaaat ggcattacga agattgagaa tcttaacacg agaaggcatt gaagatgaat
     4321 tagatttaca tactacaata gaaaaaacat gcaaaaatgc tggttattta gatattgaaa
     4381 tgcaagcgtc aaaaaagaat aaagttaaaa ttttactttt ttttgatgtc ggtggctcta
     4441 tggatcctta tattgatatt tgtgaacaac ttttctctgc tgcaaaaaca gagtttaaac
     4501 atttggagtt tttttatttc cacaactgca tttatggaag tgtctggaaa aacaatgaac
     4561 ttcgtcatca agatagaata ccaacatttg aaattttaca taaatacaat tcagattatc
     4621 gcattatttt tgttggtgat gcaacaatgt ctccttatga aattactgca aatggtggaa
     4681 gtgtagagca ttttaatgat gaaagtggtg caacttggct gaataggttt gttgaaaaat
     4741 ataagaatat tgtttggtta aatccttcta atgaatattt ttgggacgat attccttctg
     4801 ttcaaattat caaacaaata tttaaagata gaatgtatgc attaacgtta gatggtattt
     4861 caaatgctat gaaaacattg aagaaaaaca aacgtgctgt ctaatatttc tgcaacttaa
     4921 aaactatgac ttatctatca atttctttgg aagttggtat agttaaatac tttgagttta
     4981 ataatatggc taacaatatg attggaacta cagcggcatg gtattgctga ggatttaaac
     5041 cccaaccaca caacaaaata accaatatca ttctataaac aaagaaatat tgatgcaccc
     5101 attttctata tttattggat aatataaata atgcaattgg taaattaagt ggcattgccc
     5161 ataatatatt caaattccaa taggttgtcg tgtgatttgt accaaaccaa aaataaatga
     5221 ataaccaacc taataatcct aatgttgtaa aaaaagttac ggctactact ttatacaaaa
     5281 cagcattaaa tttatttttg atttgtaaaa taaaaaagaa caagaatatc aaccaaaaca
     5341 acaaacctgg cgtaaaccac tttttactat gatgctccgg gattgtatct aaaatttgtg
     5401 aggaactcaa gacaattgat tttccattta atgttgcccc ttcgattaat tgttgcaatt
     5461 caaaaggtaa aaaagtcctt ccataacctg cttttttatc cgttggcaaa cctattgcta
     5521 tatccattcc aaaatccaac cattcattgg aattatgtcg atcaattaat tcacgatagg
     5581 tagcctcatc atctacatct ggtttgaatt tcaattggtc tttgaagagt actttcacga
     5641 taacatctcg tggtcgtgtt gcacaattat catacaaaaa atcatagcga taatacttaa
     5701 attcctctcg agcattcgtg gttaaaaact caaatatttt ttgaacctga gttgaatcta
     5761 aatctaatac ttgttcatga actgagcgat tttcttcaac atattcattc ataaaagcgg
     5821 taaaatcatc tactgaaaga aaataatcta actctccttt tataaatttt ttaataaagt
     5881 ttggatcata aaaatcaaat gtgccataat tatagaccaa atcccaacct aatttttcat
     5941 cttttatacg tatgcctgta tgaccaaaaa gttggtatat ttcttcgccg acaccaacag
     6001 ttaacaaact aatctttgca tctgaactga ttttaggaac attaaaattt tgtacttgtg
     6061 ctttgctaaa gccgaaaaat aaaaaaaaca aaatcaaact aagtccaaat ctcatctgca
     6121 atatataaaa gaagtacaaa attaatttat tttcaatgat tttagcatat aaatcggaaa
     6181 tatctataaa aaaaatactt acatttagaa tatgaaaact aaagaagaga ttgtacacaa
     6241 ttggttgcca cgttatacag gtaagaaact tgaagaattt ggagagtata ttttattgac
     6301 taattttgag aaatatatag aattatttgc tgaaattcat caagttgaag tcgatggatt
     6361 aaatagtcca atgccaaatg ctactgctgg aaatataacg attatcaact tcggaatggg
     6421 gagtgccgtc gctgcaacaa ttttagattt gctttctgcc atcaagccaa aagctgcact
     6481 gtttttaggc aaatgtggtg gactgaaaaa aaagaatgaa attggcgatt ttgtcttacc
     6541 aattgctgca attcgtggcg atggtacaag caatgattat tttcctccag aagttccggc
     6601 attgccatca tttgcactac aaaaagctat ttccacgacg attcgtgagt ttcagcaaga
     6661 ttattggact ggaacagttt ataccaccaa ccgaagagtt tgggaacatg atgatacttt
     6721 taaagagtat ttacgtaaac ttcgtgctat ggctattgat atggaaactg ctacaatttt
     6781 tagtgttgct tttgccaatg aaattccagt tggtgcattg ctattagttt ccgaccaacc
     6841 tatgattcct gaaggtataa aaactagtga aagtgataaa aatgtaacca aaaattatgt
     6901 tgatttacat ttaaaaattg gcattgcatc tttgcaacaa ttacaaaata aaaccaccac
     6961 gattaagcac ttacgcttcg aagattaaaa acaaaaagca ccatcttacg atgatgctct
     7021 ttgctcattc gtatttttat tactgatatt gttgaataat ttttttcaat tctgctttct
     7081 ctatagttcc attatgtttc caaacttctt taccttcttt atataaaact aataatggga
     7141 ttgctgctat ttttagatgg cttaccacct ctcctgcttc atcataatta attttctgca
     7201 atttgaaagt acccatttct gtttctactt cttttacaat tggagaaagt attttgcatg
     7261 gcccacacca aggtgcataa aaatctacta ataccatttt atccgcactt accaatttat
     7321 tataatcagc caaattcaaa cctttgtttg cgttactttc ttgcgttaca ggtttttctg
     7381 ctcctttcca agcaataatt ccacctttta aattatatac ttctttaaat ccttgctgac
     7441 ttaaataaga agcagccttc gcacttcttg ctcctgaaag acaatacaga tacacaggct
     7501 tatttttatc taactttgca acttcatctg caaaacttgc accattgata ttaatatttt
     7561 gagcattttc taaatgtcct gaagcatact cttcttctgt tcttacatct aataaatgaa
     7621 ttcctgaatt gcctacaact ttttcagcaa attgattaac atctaaattt tcattgatgc
     7681 tttgtgttgt gccattatct tgcgaggcat tgccattatt acaagcaaca aatccaagca
     7741 caaaaacgag tataaaataa tattttttca taagtcaaaa ttaatctaat attaggataa
     7801 gcagtgcaac aaaagttaca caacatttta tttttattta ctcaaatttg taaccgattt
     7861 aaacattcta ttccacttaa ttgattttgg tatttgttca aaattattca tagataacca
     7921 aacaaacaat ggctttccaa cgatatggtc ttctggcaca aagccccaaa atcttgaatc
     7981 agcggaatta tgtcgattat cgcccatcat aaaataataa tcttgtgtaa acgtaaattg
     8041 tgttgccaat tttccatcaa tgataaattg tctattatta tttacttcta tcgaatgttt
     8101 ttcaaaatat tttatcgttg aataatattt tacaaaattt tcagcagtca aatcaattgt
     8161 catgtctttt ttaggaatca ctaaaggccc aaaattatcg actgtccaac ccaattgact
     8221 cggagctata aaaatatctt ccaaaaattg aatgggttgt tcttgtttaa attcggctat
     8281 ggtttgaatt aagttttttt gcttcatcaa ttctatttga ttatctgtca agaatatcaa
     8341 aacactatcc gactgagaaa tataattatc aaaatcttct tcaatttgat tcaatggtgc
     8401 tattggattg ccataaccat cagcataatt tattgcaaaa tcataaaacc ataataagcc
     8461 taactcatca atcaatggac gatttaattt ttgttttaaa actacactat acaaaaactg
     8521 catattggta taatgtgctt gtttttcatt attgataaac aattgacgat tgataatttg
     8581 caaagtatcg cccggtgtac caacacatcg ttttacataa ttttctcttt tatctatcgg
     8641 acgttctgga tgttctaccg catcgcctgg ataattaaaa accactacat ctccttttgt
     8701 tactttttca aaacctggca atcttttgta aggcaactga attaatgtgg aataagcctt
     8761 gcctaaacca agtggcatgg tatgatgtgc aaatggaaat gcaatgggcg tttgtgggat
     8821 tcttgctcca taattaaatt ttgaaacaaa taaaaaatcg cctgccaata atgaaccttc
     8881 catcgaagtg gttggtattt tatatgcttc caaagtaaac attctcacaa aagttgcagc
     8941 aattacagca aatacaattg catcgcccca ttctcttgcc atatttcgtt tatgtttgaa
     9001 atttttatca gcgtataatg gtcctaaata ttttgcattt ttatctacaa aaccaatata
     9061 actaaacgaa ataaaaccaa ataatgaagc aaaagtatgg tgtaaaaaag aggtttttcc
     9121 aaaacaattt aaggtatcag ttacccacgt taatgcaaat agaaaattca catacggaat
     9181 caaaccccaa aatgctttaa gtttattttt accaataatt tctaaccaag tagcatcttg
     9241 taaaaaagga ataaatacat gtataggatt ttttcctgcc tttttaaaaa taccatataa
     9301 agaaaaatgt aggaaataat acaaaagata aaagtaaata atactcagca tagaatgatt
     9361 ttaactggac aaagatgcta attttttcaa taaaataagt aatcaatttc taaaataagg
     9421 ttatttaacc actaaaaact ttgttttttt acagcttaca atctctatta tttatatctt
     9481 tgcgagccaa tggcaaaaaa agctactcat atcgaattgg tcaatcgtaa agctagtttc
     9541 aattacgaac ttttagagtc gtataaagct ggtattattc tcgctggaac tgaaataaaa
     9601 tctattcgag aaggcaaagc caatttatca gatagttatt gtttttttaa agatgatgaa
     9661 ctttgggtaa aaaatttaca cattgcagaa tatagttttg gtacgtacaa caaccatgtt
     9721 cctcttcgtc aaagaaaatt gcttttacaa aaaagagaac taaaaaagtt gcactccaaa
     9781 ataaaagaaa gaggttttac gattattcct tataaattat tttttaatga acgcaattta
     9841 tgcaaacttg aaatctatct tgcaagaggt aaaaaatcgt tcgataaacg tgaaactata
     9901 aaagctcgtg atgagcaaaa aaatatagat agaatcaaaa aatcatatag atagctttga
     9961 atatctttta ctttttgtag tataatttca tctcaacagc atgatggtca gaaagtgatt
    10021 ggtgtttttt agaccaaggg ctttcaatga ttttcacttc acgttttacg tcttcaactt
    10081 ttaattgatg ttgcattaga aaaatataat ctatccaatt ttgagatttt ccataagcta
    10141 aagtattctt tgtataatca taagttaatt gtaaatctcc ttccatttta taatcattga
    10201 cattcaaaat acctagcata ctatcataca aactcctttt atatttatcg acatttaagt
    10261 cgccagctac aatcgttgga aggctatctt tttcgtattt atctaataat tctgttttaa
    10321 gttgttttaa ctgacagatt ctataatcat cggatcctgc agcattcaaa tgagtgccta
    10381 caacataaaa ttcttgtcca tttttattta gcttgaccat taaagctccc ttttttgcca
    10441 ttctatcaat gccttttttt gctttatatt ttattttatc aacctcttcg ataggaaact
    10501 ttgacattat ccaaattcca tcagatgttt taatagaaaa attactttaa aaagcgggtc
    10561 caattctata aggataatca gtctgcattc ttttaaccat tcttcgtctt gcaccacgat
    10621 gaaaagcttc ctgaaataca attaaatcat aatcagtcgt caaaagttgg ttggcgatat
    10681 gaatagaacg ctttttcttt ccagtaaatt taagcatagg cggaagcata tatatattcc
    10741 aagaaagcat ttttaaacta tctaaagcaa cttcttgctc aggtaattgt gttggattta
    10801 tgggcataat ttgagcctca gaaaaattta ttactagaat aaagaaaaaa gaaattgcga
    10861 tttttatcat ttacaaatct attaaaaaaa atctaggaac acgactattg caaatatcca
    10921 attacaaaat catccaaggc tttcattaaa ttttatacca caattattgc agtgttgttg
    10981 aagattaaga aatattgcat aacaatgaag attaaaaaga gtttggtttt aaatttataa
    11041 aaatagattt atttgcaatt atcataatac aaaatatatc aagatttaaa gtaattccta
    11101 atagatatta tagatatagt atcaaaagaa ttttctattt ttaaaaacag tagaattaaa
    11161 tattctattt ttgaatttag ctaaacaaaa gccaaaaaaa actaaaacta tcttactaag
    11221 tattttgaga attatacatt gaaaattaag taaataataa aagaaaaaat gacaagtacc
    11281 gcacaaagag ccgaattaca agccaaaata tggaaaatag ccaatgaagt acgtggagcc
    11341 gttgatggat gggattttaa acagtttgtt ctcggagctc tgttttaccg ctttatcagc
    11401 gaaaatttct ccaagcattt tgacgatgca gacacagttt atgccaattt agcggatgat
    11461 gtcatctctt cagaaataaa agacgatgcc attaaaacca aaggctattt catttatcct
    11521 tcacagcttt ttgtaaatat tgccaaatcc gcaagcacca acaaaaattt gaacaccgag
    11581 ctcaaagcta tttttgatgc cattgaaagc tctgccaatg gttatgcttc tgaagacgat
    11641 atcaaaggtt tgtttgccga ttttgatact acaagcaatc gcttgggaaa ttccgttcca
    11701 gataaaaaca aacgattggc tgccgtgcta aaaggcgtag aagaactcaa ttttggcaat
    11761 tttgaaaata atcaaatcga cctatttggt gatgcttacg agttcctaat ttccaactat
    11821 gcagccaatg ctggaaaatc aggtggtgaa ttttttacgc ctcaaacagt ttccaagctc
    11881 attgctcaat tggctttgca caagaaaaaa aaggtaaata aaatttacga ccccgcctgt
    11941 ggctcaggtt cattgctctt gcaagccaaa aaacatttcg acagtcatat tattgaagaa
    12001 ggattttttg gacaagaaat caaccatacc acttataact tggctcgtat gaatatgttt
    12061 ttgcacaata tcaattacga taaattccat attttgctag gcgatacact tttaaaacct
    12121 gcattgggcg acgaaaaacc ttttgatgcc attgtttcta atcctccata ctcggtaact
    12181 tggattggta gtgatgaccc tactttaatt aatgacgacc gttttgctcc ggcaggcgtt
    12241 ttagctccca aatctaaagc cgattttgcc tttgtattgc acgctttaag ttatctatct
    12301 agcaaaggaa gagctgccat tgtatgcttt ccaggtattt tttaccgtgg cggtgccgag
    12361 cagaaaatcc gtcagtattt ggtggataat aattttgtag aaacagtaat ttcccttgct
    12421 cccaacttgt tttttggcac ttcaatagcc gtaaatattt tggtactttc caaaaacaaa
    12481 aaagaaaata gcacccagtt tattgatgca agtggcgaag atttcttcaa aaaagtaact
    12541 aataataatg tattggaaga cagccacatt gaacgcatta tggaactgtt cgacaaaaaa
    12601 gaaaatgttg cacataccgc catcactgtt gaaaacagca aaattgccga aaatgattac
    12661 aatctttccg taagctctta tgtagaagcc aaagacaatc gagaagtgat tgatataaaa
    12721 gtgttgaatg cagagattgc tgaaacggta aagaaaataa atgctttgcg tacggatatt
    12781 gataaaatta ttaaagagat agaaactgtc taatccttga tttttgtgat tcaattatcc
    12841 agatgattca ataaaaaaaa gaatcagtta tatcacagca ataaaaaaaa tcacaataca
    12901 aaaaaaacaa atatgccata caatccccaa aaacaccatc gtcgttctat ccgattaaaa
    12961 ggatatgatt atgcacaggc aggattgtat tttattacaa tatgttgcca agatagagca
    13021 aatttatttg gagcaataga aaacggtgaa atgatattaa atgaatacgg acaaattgca
    13081 ttcgaagaat gggcaaacac cgaaaacatt cgagataatt gccgcatcca cgaatacatc
    13141 atcatgccca atcatatcca tggaatcatc gaaattattt ttaaaaaaga aaataatatg
    13201 gacgaagctt taatttctaa taattcgaaa gaagagaatc ttaatagtag tacgggcgaa
    13261 gagattattt ctgataataa gagcgaatca attatttccg aaaatacggg caaagagatt
    13321 atttctgata ataagagcga atcaattatt tccgagaata agagcgaatc aattatttcc
    13381 gataatacgg gcgaagagat tatttccgat aatacgggcg aagagattat ttctgataat
    13441 aagagcgaat caattttttt cgatagtacg ggcgaattgc aattcgcccc aaccgcccca
    13501 acggaattcc ccccaaccgc cccaaaattc aaatccccat ctcaaactat cggctccata
    13561 ataagaggat ttaaaatcgc tacaataaaa agaataaaag atttaatttt aaaggaaaag
    13621 gataatatga gcgaagctct aatttctaat aattcggaag aaaagaatct taatagtagt
    13681 acgggcgaat tgcaatttgc cccaatggaa ttcgcccaaa cggaattcgc cccaaccgcc
    13741 ccaacggaat tcgcctcaac cgcaccaata atccccaata attcagaaga ttctataatt
    13801 tctaataata agagcaaaga gattatttcc gataataaga gcaaatcaat tttttccgat
    13861 agtacgggcg aattgcaatt cgccccaacg gaattcgccc caacggaatt cgccccaacc
    13921 gcaccaataa ttaccaataa ttcagaagat tctataattt ctaataataa gagcaaagag
    13981 attatttc
//
LOCUS       DAOQCW010000066        13983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1612_length_13983_cov_5.684951, whole genome shotgun sequence.
ACCESSION   DAOQCW010000066 DAOQCW010000000
VERSION     DAOQCW010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13983
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1612_length_13983_cov_5.684951"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(269..859)
                     /locus_tag="PLS16_07180"
     CDS             complement(269..859)
                     /locus_tag="PLS16_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60901.1"
                     /translation="MEIKINHTNSLSAARYFSSFGIQNFGFTLDSMNPNHISLLDAKQ
                     IVSWLYQPNIILEVGIFQNIEEIEILQKEFHFSSVELNIHHPDFKRIIEQYNQSWIKL
                     NIDELQQIDFNEPIFQDSAFIINIFELKIDFKMLIEFSQKHLILIDLNDSSKIEDFIK
                     KTGIMNYTIPCVREKSVGKLDLEPYEHFLPYLEDAY"
     gene            complement(861..1901)
                     /locus_tag="PLS16_07185"
     CDS             complement(861..1901)
                     /locus_tag="PLS16_07185"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNJ60902.1"
                     /translation="METSKREKIIGIWLMIGVFMLMVQIFLGGVTRLTGSGLSITEWD
                     AIMGFIPPLNQAEWVEVFEKYKQFDQFRLVNSTFTLADFKSIFLWEFVHRLWARFMAL
                     CAFLPMIYFIFKGILKQKDILKLLLIIALGGLAGFLGWIMVASGLEKNKVLVNPAKLM
                     LHLLVAAFIVSLTFRFALGYLYPRNQTTLNSTRNIISGFLILIFIQICLGALVAGSKA
                     AFSCTTFPLMHGAFIPDNLGFPQPYENFIHENNFTLQFIHRMTAYFIAIVSIVLFFKF
                     SKLSFINAFHRSRKILIIAVFIQIGLGAATLLFTKSSIPVFWAELHQLGAFAILFSVI
                     ALHYFSQPLNKL"
     gene            complement(1888..2871)
                     /locus_tag="PLS16_07190"
     CDS             complement(1888..2871)
                     /locus_tag="PLS16_07190"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNJ60903.1"
                     /translation="MVLVTGASGFLGGELVKKLVANGESVRIIRRNNSDLAHLKSIID
                     KIEVKEADILDVSALEDAFENIDQVYHTAAIISFNKQNIAQMYKTNIEGTANVVNVAL
                     SSNLKKLIHISSIAAIGGKPNELITEETKWEDNEWTSNYGITKQMAERQIFRGIEEGL
                     EATIINPGIIVGIGHEENKSTLNLLNRISHQKMPFYTNGTNGFVDIDDVVNISIQLMQ
                     GNYNKEKFIAISENWSFKQYFESIARALGVPAPKRALNTWTSSIFYNGDALLSFLNPN
                     RKRSFTKENAKIAMEQFSYSNQKVKNALNYNFTPMQETIHKIVKKYQDGNK"
     gene            complement(2881..4041)
                     /locus_tag="PLS16_07195"
     CDS             complement(2881..4041)
                     /locus_tag="PLS16_07195"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HNJ60904.1"
                     /translation="MKLEKTPTIGVIGGGQLGKMLIQKAMQWSVPIIILDKGKDCVAS
                     NYAQQHIVKDIKNADAIQELSDQVDVVTIEIEHTNADKLIELHNQGKTIIPNPYVLKT
                     IQDKGTQKVFYQENNIPTATFFLAENKSELLEKLNSFDLEKVVVKSRTGGYDGKGVYI
                     NSKKNILDNIDIIPFDGSMMIEAHVPFKKELAVLAAQDIHGNIVSYPTIEMEFDPKSN
                     LVNFLFSPANIPIHIEEKAALIAKQCLSSFNSVGLFAIELFLTEDDQLLVNEIAPRPH
                     NSAHHTIEGFYTSQYEQLLRVLLGFPLGNTQYKMPSAMLNIVASETQSGHYVLKYFKE
                     LSAIKGVYVHLYGKEFAKPNRKLGHITICLPSIEEVMKTAYQIKNLLEIEII"
     gene            4223..5704
                     /locus_tag="PLS16_07200"
     CDS             4223..5704
                     /locus_tag="PLS16_07200"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HNJ60905.1"
                     /translation="MLKINQKIEADNQIIIIDALQEDHLAFLEKEEALIVQKKIEEKQ
                     NPILIQALNKNYILYIFKAEKLNAQTKEKLRRDAHKIYQELQSKKEKNVSVQCITKIE
                     PKFIKAFLEGMLLSQYVFDKYKKNKKELVEIFVGNQLLSKADFQELNILQEAVKITKD
                     LVNEPQNIMNAVHYTNEIKKIFVKSNVKYTYWNQEKITKEKMGGILAVNRGSENPAFF
                     HILEYKPTNALNKQPLVLVGKGVMYDTGGLSIKPTPNSMDLMKCDMAGSATVLGTILA
                     ISKLNLPYYIVGLFPTVENKINEKSICPGDVITMYDGTTVEVMNTDAEGRLILADALH
                     YAKRLNPELVIDLATLTGAAIRAVGKEAAVVMGNADKKIRKKLEKVAYKTDEKIVYFP
                     LWDDYKPYLDSEIADLKNIGGAEAGAITAGKFLEHFTDYPWLHIDLSPAFVTSRYNYR
                     GIHATAYGVRLLCKFVKNLSKQQKQIRNVKSQPRKNKSRNNNR"
     gene            5655..6725
                     /gene="pdxA"
                     /locus_tag="PLS16_07205"
     CDS             5655..6725
                     /gene="pdxA"
                     /locus_tag="PLS16_07205"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNJ60906.1"
                     /translation="MSNPNLEKIRVGITIGDAAGVGPEIIMKTFSDDRLFKQFTPIVF
                     GNPRVFSFYKKVLNIEKFQYSSIKDYKNLSNNSINIVPVWEDEFVISPGQPSNETGKF
                     AFLSLKAACEALNNGDIDVLVTAPINKNVIQSAEFNNTGHTEYLQKFFGAKDNLMFLV
                     ADDIKVGLVTNHEPISEVAKNITKEKIQRKIQLINKSLQEDFGIDKPKIAVLGLNPHA
                     GDKGLVGQEELEIIFPAVKEMRDQQNIIAFGPYPADGFFASDNWRKFDAILAMYHDQG
                     LIPFKYIAGAEGVNFTAGLSVVRTSPDHGTAENIAGKGIADEISFRNAIYKAVDIFNN
                     RADYAEMRSNPLKKLAKFEEER"
     gene            complement(6748..7248)
                     /locus_tag="PLS16_07210"
     CDS             complement(6748..7248)
                     /locus_tag="PLS16_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNJ60907.1"
                     /translation="MKIGIISDTHSYLDKQVLHYFKDVDEIWHAGDIGNLEVTNALVQ
                     EKKLRAVFGNIDNAEARMQFAKFESFSIENCKILIIHIAGSFGKYNKETQDLIKEHQP
                     NILVCGHSHIVKVANDIKYNLLYINPGAAGIHGFHKVKTIIRFEIENGKPKNMELIEL
                     GQRGKV"
     gene            complement(7252..8148)
                     /locus_tag="PLS16_07215"
     CDS             complement(7252..8148)
                     /locus_tag="PLS16_07215"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HNJ60908.1"
                     /translation="MNEIDQIQVNFSENLWVLNICLSFIMFSVALDLKVSDFKSIIQH
                     PKIALAGIFSQYIAFPFVAFLFIKITQPHPTIALGLLLVVVCPSGNISNFLTHTAKGN
                     IALAISLTGISTILAPISAPFLFTFYANMDAGMQSILKNFHIGFLEMLQSVSVLLLFP
                     ILLALLLNYKFPNFVLRIKKTVKIFSLLIFIAFLIFAFKANFEVFKKYVSLVLWVVFF
                     LDLLGFIIGYYTGKLFRLNYSDCKTLSIETGIHNAGLGLVLIFSFFGGIGGMALVAAW
                     WGIWHLFAGMAIAQYWKRKAEN"
     gene            complement(8158..9195)
                     /locus_tag="PLS16_07220"
     CDS             complement(8158..9195)
                     /locus_tag="PLS16_07220"
                     /inference="COORDINATES: protein motif:HMM:NF014698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HNJ60909.1"
                     /translation="MKTFFLENGKTYPLVVAPETDNSLEGLKNWIAKNHEDLEQKLLH
                     YGAILFRGFDIQAPLDFEQVAKSIDADLKNDYMGTSPRDKKTDFVFSASELPPHYPIM
                     QHCEMSFLPSAPRRLFFYCHTAPPFGGETPICDFRKVYKDLKTEVKNAFETKGVKHIR
                     NYASPNTKARTGFQLKSWHDMFHTDDKKIVEEKCKEHDIICEWKENEGLRMTNKSNAF
                     KTHPVTGEKAWFNHTQVFHIDAAAIEYDYIHKRQKRWETFKTELLLELMTFYKKLATD
                     SNDQSMHVTFGDGTEIPKDYVQHIQDVIWENMSIYSWQKGDIIAIDNFSTSHGRLPYY
                     GDREVMVCWTS"
     gene            9249..9800
                     /locus_tag="PLS16_07225"
     CDS             9249..9800
                     /locus_tag="PLS16_07225"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNJ60910.1"
                     /translation="MKYCPKKERVTIGDSIFLVPYQNGFEFDFFNLVQKNIDRLKDSF
                     PNMVRKNETVDGTKSYFEEKNIFWNKNIEYANLIFFEELLIGHFNIKDINIKKSTVEL
                     SYFIDKDFEKRGIVFQIIENRKKFIFNQLNIKTIKARCNINNKESEKVMQRAGMQYEG
                     TIYQNYKTYDEMPVDTYIYSITQ"
     gene            complement(9817..10989)
                     /locus_tag="PLS16_07230"
     CDS             complement(9817..10989)
                     /locus_tag="PLS16_07230"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNJ60911.1"
                     /translation="MIKRKLQYTIQSLLSDFPAVAILGPRQIGKTTLAKSIAKQLKKP
                     TIYLDVEKPSDYAKLQDAEAYLQMHQDKCVIIDEVQFMPSLYNVLRSLIDTHRKPMRF
                     ILTGSASPNLIKGISESLAGRIAYTELSPIGLDELPRNIARNKHYFFGGFPTPLTYKD
                     TSKAIHWLDYFIKSYIERDLSMLYDVKMDTRVVRHFWQMLANNNSGLWNAETYARALG
                     VTAPTVNRYLDFLEGAYLVRRLYPWFPNTNKRLVKSPKVYIRDSGLLHRLADIYKLDE
                     LYGNVIIGASWEGYVIEQIITNLPNNITPYFYRTHAGTEVDLVLVKQNKPYILIEIKL
                     NNLSKPKRGFYEAIDDLKPTKSFVISKETETYTDSGNVTYISLYNFIMLENLKLYF"
     gene            11200..13320
                     /gene="metG"
                     /locus_tag="PLS16_07235"
     CDS             11200..13320
                     /gene="metG"
                     /locus_tag="PLS16_07235"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNJ60912.1"
                     /translation="MSKKRYTITAALPYANGPLHIGHLAGAYIAADIYARHLRLKGED
                     VIFVCGSDEHGAAITIKAKKERTTPRKIVNKYNQIIRKSFEQFGIAFDIYHRTSEPIH
                     YETSQEIFNILNDKNAFEIIENEQYYDAKANQFLADRYIIGTCPKCANENAYGDQCEK
                     CGSSLNPTDLINPKSTLTNSTPILKKTKHWFLKLDQQQDAIREWLETTAEKNNWKAHV
                     IGQCKSWLNDGLRPRAMTRDLDWGIPVPANIEGSEGKVLYVWLDAPIGYISATKQLFK
                     EIGEEKFDFAYPQNFSKYLQNKKAEDWRKYWQDEDTTLINFIGKDNIVFHCITFPAIL
                     KITEQYILTSNVPANEFMNLEGDKISTSRNWAVWLHEYLEEFPGKEDELRYCLIANMP
                     ENKDSEFTWKEYQDRVNNELVATYGNFVNRVVTLIHKYYLGTLDNSFRQTMRDNDTVL
                     SKIISETEQNIANSIEQFKFKDALNELMAFARAGNKFLADTEPWKLVKEDPDKTRSIL
                     SIALDWVYALAIFSKPLLPKSADKIFRMLNADVTDNDPFNYQPKKEHINPIVLLFEKI
                     TDEQIAVQIDKLQQPKQIQLENSGDKSSPSLPLKSEIQYDDFAKMDIRTGKILEAEKV
                     PKADKLLKLIVDIGIEQRTIVSGIAEHFKPEAIVGQQVLVLVNLAPRKLRGIESNGMI
                     LMAEDADGKLHFAQANVNAGMEVR"
     gene            13438..>13983
                     /locus_tag="PLS16_07240"
     CDS             13438..>13983
                     /locus_tag="PLS16_07240"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNJ60913.1"
                     /translation="MIQPRTFKGARDFLPEQMLHRERILDTMRTVFKKYGYAPVETPA
                     IEYLDVLTGKYGEEADRLIYKLNYKTGTKDEAALHYDLTVPFSRMVAMNNDLAMPFKR
                     YQMQPVWRADRPQPHQGRFREFYQCDVDCVGSSSMLIEAELIAITYEVLNGLDIPDFL
                     IKINNRKILNGITQYIGLDNNF"
BASE COUNT         4956 a         2020 c         2248 g         4759 t
ORIGIN      
        1 tattgcagga atttataatc taagtctaaa atgaatttcg caagaggtct attgtaatag
       61 ttaatgaaga tgatgaagaa ttaacttatt atttaacttg gttggatagt gataacctag
      121 tttatgtgta tgttgatggg aaatttccat cagaaagttg tacagggaat attaatattt
      181 atgttcaaac cgaagtagta aaataaatta actatttata ttacaattga aggcggaatt
      241 tttatattcc gcctttttta ttttgttctt agtaagcatc ttctaaataa ggtaaaaaat
      301 gctcataagg ctctaaatca agttttccaa cagacttttc tcttacacat ggaatagtat
      361 aattcataat gccagtcttt ttaataaagt cttcaatttt tgaggagtca ttgaggtcta
      421 tcaaaatcaa atgtttttgt gaaaactcaa ttagcatttt aaagtcaatt tttagttcga
      481 aaatattgat aataaaagca ctgtcttgaa atataggctc attaaaatct atttgttgta
      541 gttcatcaat atttaatttt atccaagatt gattgtattg ttcaattatt cttttaaaat
      601 cagggtgatg gatatttaac tccacagatg agaagtgaaa ttctttttgt aatatttcta
      661 tctcttctat attttgaaaa atacctactt ctaaaataat atttggctga tagagccaac
      721 ttacaatctg ttttgcatca agtaaactta tatggtttgg attcattgaa tctaatgtaa
      781 atccaaaatt ctgaatccca aaagaactaa aataccttgc agcactcaag ctattagtgt
      841 gatttatttt tatttccaat ttataattta tttaatggtt gtgaaaaata atgtaaggca
      901 ataactgaaa atagaattgc aaatgcacct aattgatgca attctgccca aaatactgga
      961 attgaagatt ttgtaaataa taaagttgct gcacctaatc caatttgaat aaatactgct
     1021 atgattaata ttttcctact tctgtgaaat gcattaataa atgatagttt tgaaaattta
     1081 aaaaataaca caatacttac gattgctata aagtaagcag tcattctgtg aatgaattgt
     1141 agtgtgaaat tattttcgtg tataaagttt tcataaggct gtggaaaacc aaggttatct
     1201 ggaataaatg caccatgcat taagggaaaa gtagtacaac taaaagctgc ttttgaacct
     1261 gcaaccaacg cacctaagca tatttgtata aaaattaaaa tcaaaaatcc agaaataata
     1321 tttctcgtag aatttagagt tgtttgattt cttggatata aatagcctaa agcaaatcta
     1381 aatgtcaaac tcactataaa agcagcaact aataaatgca acatcaattt tgcaggattt
     1441 actaatactt tatttttttc taatccgctt gcaaccatta tccaacctaa aaatccagct
     1501 aatccaccta aagcaatgat taataatagt tttaaaatgt ctttctgttt taaaattcct
     1561 ttaaaaataa aataaatcat tggtaaaaag gcacacaatg ccatgaatct tgcccataat
     1621 cgatgcacaa attcccataa aaatatactt ttaaaatcgg ctaaagtgaa tgttgaattg
     1681 actaatctaa attggtcaaa ttgcttatat ttttcaaaaa cctctaccca ttctgcttga
     1741 tttaatggcg gaataaaacc cattattgcg tcccattctg tgatagataa accagagcca
     1801 gtgagtcgtg taacgcctcc gaggaaaatt tgtaccatca acataaatac gccaatcatc
     1861 aaccaaatac ctataatttt ttctctttta cttgtttcca tcttgatatt ttttgactat
     1921 tttatgaata gtttcttgca ttggagtgaa attataattc aatgcatttt ttactttttg
     1981 attggaataa gaaaattgct ccatagcaat ttttgcattt tcttttgtaa aggaacgttt
     2041 tctatttgga tttaaaaaac ttaaaagggc atctccattg taaaatatac tagaagtcca
     2101 agtatttaat gctcttttag gtgcaggtac gcccaaagct cttgctatag attcaaaata
     2161 ttgcttaaag ctccaatttt cactaatcgc aataaatttt tctttattat aattgccttg
     2221 cattaattgg atactaatat ttacgacatc atcaatatct acaaatccat ttgttccgtt
     2281 tgtataaaaa ggcattttct ggtgagaaat tcgatttaat aaattcaacg ttgatttgtt
     2341 ttcttcgtgt ccaatcccaa caattattcc tgggttaatt attgttgctt ctaagccctc
     2401 ttcaatacca cgaaatattt gtctttctgc catttgtttt gtgattccat aatttgaagt
     2461 ccactcattg tcttcccatt ttgtttcttc tgtaatcaac tcgtttggct tgccaccaat
     2521 agcagcaata gaactgatat gaattagttt ctttaaattt gaactcaatg ccacattgac
     2581 cacatttgct gtgccttcaa tgttcgtttt gtacatttga gctatatttt gtttgttgaa
     2641 tgaaataata gcagcagtgt gataaacttg gtctatattt tcaaaggcat cttctaatgc
     2701 agatacatct agaatatcag cttctttaac ttcaatttta tcaattatag attttaaatg
     2761 tgctaaatct gaattatttc tcctgataat tcgaacagat tctccattgg caactaattt
     2821 cttgactaat tcaccaccta aaaaacctga tgcacctgtt actaaaacca aaatatctat
     2881 ttatataatt tctatttcta ataagttttt aatttgatag gctgttttca ttacttcttc
     2941 tattgatggc agacaaatag ttatatgacc taattttcga ttaggtttgg caaattcttt
     3001 accatataga tgaacataaa caccttttat tgcagataat tctttgaaat atttcagtac
     3061 ataatgacca ctttgagttt cagaagctac aatattcaac attgcacttg gcattttata
     3121 ttgtgtattg cctaatggaa atcctagtaa aacacgaagc aattgctcat attgagaagt
     3181 gtaaaatcct tcaatagtat gatgtgctga attatgtgga cgaggagcta tttcgttcac
     3241 taacaattgg tcgtcttccg taagaaataa ctctatcgca aataaaccaa cactattaaa
     3301 agaggataaa cattgtttgg ctattaatgc tgctttttct tcgatatgaa taggaatgtt
     3361 tgcaggagaa aataagaaat ttactagatt gcttttcggg tcaaactcca tttctatagt
     3421 agggtaacta acaatatttc cgtgaatgtc ttgtgcagca agaacggcta attctttttt
     3481 gaaaggaaca tgagcttcta tcatcattga accatcaaaa ggaataatat cgatattgtc
     3541 tagaatattt ttcttactat taatataaac gcctttgcca tcgtaacctc ctgttctgga
     3601 tttaacgaca actttttcaa ggtcaaaaga gtttaatttc tctagcaatt cacttttgtt
     3661 ttctgctaga aaaaacgtag ccgttggaat attgttttct tgataaaata ccttttgagt
     3721 tcctttatct tgaatagttt ttaatacata aggattagga ataatagttt ttccttgatt
     3781 gtgcaattca attagtttgt cagcatttgt atgttcaatt tcgatggtta ccacatctac
     3841 ttgatcagat aattcttgaa tagcatctgc atttttaata tcttttacaa tatgttgctg
     3901 ggcataatta gaggcaacac aatctttacc tttgtctaaa ataataattg ggacagacca
     3961 ttgcattgct ttttgaatca acatttttcc aagttgtcct ccgccaataa ccccaatagt
     4021 tggtgttttt tctaatttca taatcaattg tgaaattaag acaattttat gctgtttccg
     4081 aacttttagg gtttatttat tattatgctt atttattttg ataaagaaat tttatatggt
     4141 caattaagat tcaataaaga aagatatgcg gtatagtctt tagagaatgt tttgtatatt
     4201 tgagagttag aaagacttat atatgttgaa aataaatcaa aaaatagaag cagataatca
     4261 aatcattatt attgatgcgt tgcaagagga tcacttagct tttttagaaa aggaagaagc
     4321 ccttatcgta cagaagaaaa tagaagaaaa acaaaatcct atacttattc aggcattaaa
     4381 taaaaattat attctatata tttttaaagc tgaaaaatta aatgctcaaa ccaaagaaaa
     4441 actgaggaga gatgctcata aaatatatca agaacttcaa tcaaagaaag agaaaaatgt
     4501 ttctgttcaa tgtataacaa aaatagaacc taagtttatt aaggcttttt tagaagggat
     4561 gctgctttca cagtatgttt tcgataagta taaaaagaat aaaaaggaat tagtagaaat
     4621 atttgttggg aatcaacttt tgtctaaagc agattttcaa gaattaaata tcttacaaga
     4681 ggcggttaaa ataacaaaag atttagttaa tgagccacaa aatataatga atgctgttca
     4741 ttatacgaat gaaattaaaa agatatttgt aaaatctaat gtaaaatata cctattggaa
     4801 tcaagagaaa atcactaaag aaaaaatggg gggcattttg gcagttaatc gaggaagtga
     4861 aaatccagct ttttttcata ttttggaata caaaccaact aatgcactca acaaacaacc
     4921 cttggtttta gttggaaaag gcgtgatgta cgacactgga ggtttgtcta ttaagccaac
     4981 gcctaacagt atggatttga tgaaatgtga tatggcaggt tctgcaactg tattgggaac
     5041 tattttggca atttctaagt tgaatttacc ttattatata gtcggattat ttccaacagt
     5101 agaaaataaa atcaatgaga aatctatttg cccaggagat gtaataacta tgtacgatgg
     5161 cacaaccgtg gaggtaatga atacggatgc agaaggaaga ttaatcctag ctgatgcttt
     5221 gcattatgca aaaagattaa atcctgaatt agtcattgat ttagctacat taacaggtgc
     5281 agctattcgt gcggtgggta aagaagctgc tgttgtaatg ggaaacgcag ataaaaaaat
     5341 aagaaaaaaa cttgaaaaag ttgcctataa aactgatgag aagatagttt actttccact
     5401 ttgggatgac tataaacctt atttagattc tgaaattgca gatttgaaaa atataggtgg
     5461 agcagaagcc ggagctatta ctgcaggtaa atttttagaa cattttacag attatccatg
     5521 gctacatatt gatttatcac cagcctttgt aacttcaaga tataattaca gaggaattca
     5581 tgcaacagct tatggtgtca gattattatg taaatttgta aaaaatttaa gcaaacaaca
     5641 aaaacaaatt agaaatgtca aatcccaacc tagaaaaaat aagagtagga ataacaatag
     5701 gtgatgcagc aggtgttggt cctgaaataa taatgaaaac ttttagtgat gataggctgt
     5761 tcaagcaatt tacgccaata gtttttggaa atccaagagt tttttctttc tataaaaaag
     5821 tacttaatat tgagaagttt caatattctt caataaaaga ttataaaaat ttaagtaata
     5881 attctattaa tatcgtacct gtttgggaag atgaatttgt aattagtcca ggacaacctt
     5941 ctaatgaaac tggaaaattt gcatttctat ctttaaaagc agcttgtgag gcactaaaca
     6001 atggagatat tgatgttttg gttactgcac cgataaataa gaatgtaatt caatcagcag
     6061 aatttaataa tacaggacat accgagtatc ttcaaaagtt ttttggagcg aaagataatt
     6121 taatgttttt agttgcagat gatattaaag tgggattagt tactaatcat gagcctatta
     6181 gtgaagtcgc taaaaatata actaaagaaa aaattcaaag aaaaattcaa ttaataaata
     6241 aatctttgca agaagatttt ggaatagata aacctaaaat tgctgtactt ggattaaatc
     6301 cacatgctgg agataaaggt ttagttggac aagaagaatt ggaaattatt tttccagcag
     6361 tgaaggaaat gagagaccaa caaaatatta ttgcatttgg accttatccc gcagatggtt
     6421 tttttgcatc agataattgg agaaaattcg atgcaatatt agccatgtat cacgaccaag
     6481 gactgattcc ttttaaatat attgctggag cggaaggggt taattttaca gctggattaa
     6541 gtgttgttag aacttcgcca gatcatggaa ctgctgaaaa tattgcagga aaaggaattg
     6601 ctgatgaaat ttcttttaga aatgcaattt ataaagctgt agatattttc aataatagag
     6661 cagattatgc agaaatgcgt agtaatccat taaagaaatt agcaaagttt gaagaagaaa
     6721 ggtaatatat aaaaatagcg tttagtttta gacttttcct ctttgcccta attcaattaa
     6781 ttccatattc ttaggtttgc cattttctat ttcaaaacga ataatagttt taaccttatg
     6841 aaatccatgg atgcctgccg caccaggatt aatgtataat aaattatatt taatatcatt
     6901 cgctactttc acaatatgag aatgaccaca cactagaata ttaggttgat gttctttaat
     6961 taaatcttgc gtttctttgt tgtatttgcc aaagctacca gcaatatgaa tgataaggat
     7021 tttgcaattt tcaatggaaa aactttcaaa ttttgcaaat tgcattcttg cttcagcatt
     7081 atcgatattg ccaaatacag cacgtaattt tttttcttga actaaagcat ttgtaacttc
     7141 caaattgcca atatctccag catgccaaat ttcgtcaaca tctttaaaat agtgcagaac
     7201 ttgtttgtct aaataactat gcgtatccga aataatgcca attttcattt tttaattttc
     7261 tgcttttctt ttccaatatt gagcaattgc cattcctgca aaaagatgcc aaattcccca
     7321 ccatgctgct actaatgcca tgcctccaat gccaccaaaa aaactaaata tcaaaaccaa
     7381 tcctaatcca gcattatgta tgcctgtttc tatagaaagt gttttgcagt ctgaataatt
     7441 taacctgaat aattttccag tataataacc aatgataaag cctaataaat ctaaaaaaaa
     7501 aacaacccat aaaactaaag aaacgtattt cttaaagact tcaaaatttg ctttaaatgc
     7561 aaaaattaga aaggcaataa aaattagtag agagaaaatt tttactgttt tttttattct
     7621 taaaacaaag tttggaaact tgtaattgag tagtaaagct aatagaatcg gaaataataa
     7681 aagaacagaa acactttgta gcatctctag aaatccaata tgaaaatttt ttaaaattga
     7741 ttgcatacct gcatccatat tggcatagaa tgtgaaaaga aatggagcgg aaattggagc
     7801 taatatggta gaaattccag ttaaacttat ggctaatgca atgtttcctt tggcagtatg
     7861 tgtaagaaaa ttagaaatat taccactagg acaaaccacc actaacagta aacctaaagc
     7921 aattgttgga tgtggctgag taatttttat aaataagaaa gcaacaaaag gaaaagcaat
     7981 atattgagag aaaatacctg ctaaagcaat ttttgggtgt tggataattg atttaaaatc
     8041 tgaaactttt aagtctaaag caacactaaa cattataaaa ctcaaacaaa tattgagtac
     8101 ccataaattc tctgagaaat taacttgaat ttggtctatt tcgttcattc aagcatatta
     8161 agaagtccag cagaccatca cttctcggtc gccataatag ggtaatcttc catgtgatgt
     8221 tgagaagttg tcaatagcaa taatatctcc tttttgccaa gaatagatag acatattctc
     8281 ccaaatcaca tcttgaatat gttgcacata atcttttgga atttcagtgc catctccaaa
     8341 agttacatgc atactttggt cattagaatc tgtggctaat tttttataga aagtcattaa
     8401 ttctaaaagt agttcagttt taaaagtttc ccatcttttt tgtctcttat gaatatagtc
     8461 gtattcaatt gcagcagcat caatatggaa aacttgagta tggttaaacc aagctttttc
     8521 tccagtaact ggatgagttt tgaaggcatt acttttgttg gtcattctta ggccctcatt
     8581 ttctttccat tcgcaaatga tatcgtgttc tttgcatttt tcttcaacaa tttttttgtc
     8641 atccgtatgg aacatgtcgt gccaactttt tagctgaaac cctgttcttg cttttgtatt
     8701 tggtgatgca taatttctga tatgttttac acccttagtt tcaaaagcat ttttcacttc
     8761 agtttttaaa tctttataaa cttttctaaa atcacaaatt ggagtttcgc caccaaaagg
     8821 tggagcggta tgacaataaa aaaacaaacg tcttggtgct gaaggcaaaa aactcatttc
     8881 acaatgttgc ataattggat aatgtggagg caattcacta gcagaaaata caaagtcagt
     8941 ttttttatca cgaggagaag tccccatata atcatttttc aaatcagcat caatactttt
     9001 tgcaacttgc tcaaaatcta aaggtgcctg aatgtcaaat cctctaaaaa gaattgcacc
     9061 ataatgcaat agtttctgtt ctaaatcttc atggtttttt gcaatccaat ttttcaaacc
     9121 ctctaaggaa ttatccgttt ctggagcaac tactaaagga taagttttgc cattttctaa
     9181 gaaaaaagtc ttcataagct tgtgttagta taaatataaa cataattttt tatttacatt
     9241 tgttttgaat gaaatattgt ccaaaaaaag aacgtgtaac gataggagat tctatttttt
     9301 tagtgccata ccaaaatgga tttgaatttg atttttttaa tcttgtccaa aagaatatag
     9361 acagattgaa agatagtttt cccaatatgg ttaggaaaaa tgaaacagta gatggaacta
     9421 aaagctattt tgaagaaaaa aatatttttt ggaataagaa tatagaatat gccaacctaa
     9481 ttttttttga agaattattg ataggacact ttaatatcaa ggatataaat attaagaaaa
     9541 gcacagttga attatcctat tttattgata aggacttcga aaaaagaggt attgtatttc
     9601 aaattattga aaatagaaag aaatttattt ttaatcaatt aaatattaaa accattaaag
     9661 ctcgttgtaa tattaataat aaagagagtg aaaaagtaat gcaacgtgca ggaatgcaat
     9721 acgaaggtac aatttatcag aattacaaaa cttacgatga aatgcctgta gatacttaca
     9781 tttattcaat tactcaataa tataaatgct tgaaaatcaa aaatacaatt ttagattttc
     9841 aagcataata aaattgtata aacttatata agttacattc ccagagtctg tgtaggtttc
     9901 agtttctttt gaaattacaa atgatttagt aggctttaag tcatcaatag cttcatagaa
     9961 accacgtttg ggtttgctta ggttattgag tttgatttca atcaaaatat agggtttgtt
    10021 ttgtttaact aaaaccaaat ctacttcagt tcctgcatgt gtcctataaa aatagggcgt
    10081 aatattattg ggtaagttgg taataatttg ttctatgaca taaccttccc aagatgctcc
    10141 tattattaca tttccatata gttcatcaag cttataaata tctgccaaac ggtgcaataa
    10201 tccgctatct cgtatatata ctttaggcga tttcactagt cgtttatttg tatttggaaa
    10261 ccatggatag agtcgtctaa ctaaataagc accttctaag aaatctaggt atcgattgac
    10321 agttggagca gtaacaccta aagctcttgc atacgtttca gcattccaca agccactatt
    10381 gttattggct aacatttgcc agaaatgtcg cacaactctt gtgtccattt ttacatcgta
    10441 caacatactt aaatctcttt caatataact cttgatgaaa tagtctaacc agtgaatagc
    10501 ttttgaagtg tctttatagg tgagtggagt gggaaagcct ccaaaaaaat aatgtttatt
    10561 tctagcaata tttctaggta attcatctaa tccaatagga cttaattcag tataggcaat
    10621 tcttccagca agagattctg aaattccttt tattaaattc ggagatgctg agccagttaa
    10681 aataaaacgc attggttttc tatgcgtatc tataagtgaa cgcaatacgt tgtaaagtga
    10741 aggcataaat tgaacttcat caataataac acatttgtct tggtgcattt gtaaataagc
    10801 ttccgcatct tgcagtttag cataatctga tggcttctct acatctaaat aaatggtagg
    10861 cttttttaat tgctttgcaa tgctttttgc taatgttgtt tttccaattt gccttggtcc
    10921 caaaattgca acagctggaa aatcacttag tagagactga atcgtatatt gtaatttccg
    10981 ctttatcata gttcaaaagt acatattatt tttggattac taaaattttt atgcttgaaa
    11041 attaaaaatt ttatttttga ttttcaagca ttattctaag ttgatacttt ctatactttt
    11101 gataatatta tataatatta tggcggtaac ttattttgat agaaaaatca atagttgatg
    11161 tttttttcag aatttttttc taagtatctt tgttttctaa tgagtaaaaa aagatataca
    11221 attacagcgg ctttgcctta tgcaaatggg cctttacata tcggacattt agctggggct
    11281 tatatagctg cagatattta tgcacgtcat cttagattaa aaggagaaga tgttattttt
    11341 gtatgtggta gtgatgaaca tggtgctgca attactatca aagcaaaaaa agagcgtaca
    11401 acaccaagaa aaattgtcaa taaatacaat caaattatta gaaaaagttt tgagcaattt
    11461 ggaattgcat ttgatattta tcacaggact tcagagccaa ttcattatga aacttctcaa
    11521 gagattttta atattttaaa tgataaaaat gcatttgaaa tcattgaaaa tgaacaatat
    11581 tacgatgcta aagcaaatca gtttttggca gatagataca ttataggaac ttgccccaaa
    11641 tgtgcaaatg aaaatgctta tggtgatcaa tgtgagaaat gcggtagctc tttaaatcca
    11701 actgatttga ttaatcccaa atcgacgcta acaaattcaa cgcctatttt gaaaaaaacc
    11761 aaacattggt ttttgaaatt agatcaacag caagatgcaa taagagaatg gttggaaaca
    11821 acagcagaaa aaaataattg gaaagcacac gttattggtc aatgcaaaag ttggttaaac
    11881 gatggacttc gtcctagagc gatgactaga gatttagact ggggaattcc tgttcctgct
    11941 aatattgagg gttcggaagg taaagtcttg tatgtttggt tagatgcacc aataggttat
    12001 atttctgcaa ccaagcaatt gtttaaagag attggagaag aaaaatttga ttttgcttat
    12061 cctcaaaatt ttagcaagta tttgcaaaat aaaaaagctg aagattggcg taagtattgg
    12121 caagatgaag atacaacact tatcaatttt attggaaaag ataatattgt ttttcattgt
    12181 attacttttc ctgctatttt aaaaatcaca gagcaatata ttctgacaag taatgtgcca
    12241 gcgaatgaat ttatgaattt agaaggagat aaaatttcaa cttcaagaaa ttgggcagtt
    12301 tggttgcatg aatatttaga ggaatttcca ggaaaggaag atgaattacg ttactgtttg
    12361 attgcgaata tgcctgaaaa taaagattct gagtttactt ggaaagagta tcaagataga
    12421 gtgaataatg aactggtggc aacctatgga aattttgtta atcgtgtagt tacacttatt
    12481 cataaatatt atttaggaac gttagataac tcatttagac aaacaatgcg tgataatgac
    12541 acggttttaa gtaaaattat ttctgaaaca gaacaaaata ttgcaaattc tatagaacaa
    12601 tttaagttta aagatgcttt gaatgaatta atggcttttg cacgtgctgg aaataagttt
    12661 ttagcagata ctgagccttg gaaattagta aaagaagatc cagataaaac gagaagtatt
    12721 ttatcaattg cgttggattg ggtatatgca ttagctatat tttcaaaacc acttttgcca
    12781 aaatcagcag ataagatatt taggatgttg aatgctgatg taacagataa tgacccattt
    12841 aattatcaac caaagaaaga acatataaat ccgatagttt tgttatttga aaaaattaca
    12901 gatgaacaaa ttgctgtcca gatagataaa ttacaacaac ctaaacaaat tcagcttgaa
    12961 aattcaggcg ataaatcttc tccttcttta cctcttaaat cagaaattca atacgatgac
    13021 tttgctaaga tggatattag aacaggcaaa attttagaag cagagaaagt gccaaaagca
    13081 gataaattat tgaaacttat agttgatatt gggattgagc aacgtactat tgtttctgga
    13141 attgcagaac attttaaacc agaagctatt gttggtcagc aagtattagt tttagtcaat
    13201 ttagctccaa gaaaattaag aggaattgaa agtaatggca tgattttaat ggctgaagat
    13261 gctgatggaa aattgcattt tgctcaagca aatgttaatg ctggaatgga agtgagatag
    13321 ttgttactac tcgtttatgc attttatatt aaatactaca aaaacattaa atagtagata
    13381 atagactgtt atgcagttta attttaatat ttttgcttaa ataattgaac agttattatg
    13441 atacaaccaa gaacattcaa gggagcgaga gattttttgc cagagcaaat gttacataga
    13501 gaacgtattt tggatacaat gcgtactgta tttaagaaat atggctacgc acctgtagaa
    13561 acaccagcta ttgaatattt agatgttctt actggaaaat atggagagga ggcagaccgt
    13621 ttaatttata aattgaacta taaaacaggc acaaaagatg aagccgcttt gcattatgat
    13681 ttaacagtac cattttcaag aatggtggca atgaataatg atttggcaat gccatttaaa
    13741 agataccaaa tgcaaccagt ttggcgagca gacagaccac agccacatca aggaagattt
    13801 agagagtttt accaatgtga tgtagattgt gtgggatctt catctatgct gattgaagcg
    13861 gaattaattg caattactta tgaagtttta aatggcttag acattcctga ttttttaata
    13921 aaaatcaaca ataggaaaat attaaatggc atcacacaat atattggatt ggataataat
    13981 ttt
//
LOCUS       DAOQCW010000067        13573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1686_length_13573_cov_5.451768, whole genome shotgun sequence.
ACCESSION   DAOQCW010000067 DAOQCW010000000
VERSION     DAOQCW010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13573
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1686_length_13573_cov_5.451768"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1327
                     /locus_tag="PLS16_07245"
     CDS             <1..1327
                     /locus_tag="PLS16_07245"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNJ60914.1"
                     /translation="GGILFALGHFVFIPFLRYVSTSGMSELLTASSLFLVIGVSELMV
                     AVGLSPALGAFIAGMMLANSEFRNELESNIEPMKGILLAVFFVSVGAMINFNIIFDDY
                     ILIFSLVGLVLVVKFGVLCLIGKIYKIDFVQNLFYAFTLSQIGEFAFVLLGFSKEVSI
                     FNNQLYAQLMAVTTITMLTTPILLIIYDKFISPSIKPKNTDESDFDIQKMDIQKKKII
                     IVGFGFFGSTVGRLLKANKISATILDHDTERVKILREYGFKVYYGDATRWSILETSGI
                     HEAEILVLCLDNPEENMRIIELVKKKKLNLKIFVRARNRLDAELFFKNGIDNFYRETM
                     GTAVNMAVDVLHEMGMRKYTARRMGKRFELIDEASLKKMAEENNLDASFTMKETIQRE
                     QLLLSEDSRNFESYQLIEENQEDENENLEDEITNSSKPSNEDNAQLNLF"
     gene            complement(1330..1890)
                     /locus_tag="PLS16_07250"
     CDS             complement(1330..1890)
                     /locus_tag="PLS16_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60915.1"
                     /translation="MKHLSDFFYSKIQQKSLWIMLFLYTVIGFGIIPYKTAQMQQQYG
                     EHLMPFDLTFGYDKAFADSFLDQLGENGRVEYVQFVKIYDSIYPFIYGGLLLTMLTLL
                     INRGGYAHSKWRVLNLLPILIVLVDFGENVCSIYNTTHFPMYSLTLLKIGSVLTQIKW
                     SLVLLTFIALIYFGFKAFRRRSNISK"
     gene            complement(1911..2573)
                     /locus_tag="PLS16_07255"
     CDS             complement(1911..2573)
                     /locus_tag="PLS16_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60916.1"
                     /translation="MSAANALIFSILAFVVLTTISMLCYPGGKFFDHSSIGYSFLENF
                     YSDLGRYKTFSGGNKNISTFFFVIAVFQMSWFIANFFKAFISKVAHIDNHYSIQKLVR
                     TSAFAYGILLASTAITPYDRLLLVHALVANFSFVAMTFVAFGTAFLTYKINFPKSYTF
                     VFVLLGMLLVVYIILLFTVRSLVQGANPYFHPTAQKIMVVCIISALVYLASGYKKFVD
                     KY"
     gene            complement(2578..3567)
                     /gene="floA"
                     /locus_tag="PLS16_07260"
     CDS             complement(2578..3567)
                     /gene="floA"
                     /locus_tag="PLS16_07260"
                     /inference="COORDINATES: protein motif:HMM:NF010186.0"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HNJ60917.1"
                     /translation="MSGTFLTVITIVLLLFILIFVFTFMRYISLWVSARLSNVNIGLI
                     QLFVMQLRKVPPATIVNSMITATKAGIPVDRDGLEAHFLAGGNVMKVVSALISADKAN
                     ISLTFKEATAIDLAGRDVFEAVQLSVNPKVIESPPVTAVAKDGIQLIAKARVTVRANI
                     RQLVGGAGEETVLARIGEGIVSSIGSSADHKQVLENPDSISKVVLNKGLDSGTAFEIL
                     SIDIADIDIGKNIGAILQMDQANADKNIAQAKAEERRAMAVAQEHEMIAKAQEAKAKV
                     IEAEAEIPMAIAEAFKNGNLGIMDYYKIENVKADTKMRDSIGGGDGKDKKGSI"
     gene            complement(3579..4043)
                     /locus_tag="PLS16_07265"
     CDS             complement(3579..4043)
                     /locus_tag="PLS16_07265"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNJ60918.1"
                     /translation="MSPLAIVIGILLFGTLLFVVEIFVMPTLIIGKVAFVVTLGGLAL
                     AFYELGALYGSLCVLAAIIINGLLLYFGMERIAKSKISVLEVIDGKVNNFEDFGLKID
                     DEGVAVTDIRPEGKAIFNDQKVTVWALTGFITTETKIQIHKIEENKIFVKPI"
     gene            4116..4583
                     /locus_tag="PLS16_07270"
     CDS             4116..4583
                     /locus_tag="PLS16_07270"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNJ60919.1"
                     /translation="MKIEFWVIGKNSDKFIDDGIQAYLKKIKYFNDIQFHIFSDIKNI
                     KETNVLKDKEAEKYLSKIEPDDCVILLDENGSTYSSRKFAHFIEQRQNQSTRRCIFII
                     GGAFGFNDKLYKRSNYKISLSEMTFSHQLIRLIFLEQLYRAYTIINNFPYHND"
     gene            complement(4580..4978)
                     /locus_tag="PLS16_07275"
     CDS             complement(4580..4978)
                     /locus_tag="PLS16_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60920.1"
                     /translation="MKWIRILQPTLLVFIGVVSVFMTSSVIFDWFGIRAKEGNFVPFI
                     VYTNFICGWIYLFAAYESWKHQWIGVRLLLLASIALIVAFVFLQLHIYNGGLYEIKTV
                     KAMLFRITFTITMTVAGFYLLKHRNRTSKN"
     gene            5292..7862
                     /locus_tag="PLS16_07280"
     CDS             5292..7862
                     /locus_tag="PLS16_07280"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HNJ60921.1"
                     /translation="METATLEPKNKKITYETANNEALRYFKGDDLAAKVWVTKYALKD
                     SDGNLYEANPDDMHWRIANEIARIETRYPNPMSAQEIFDLIKDFKYIIPQGSPMTGIG
                     NNFQVASLSNCFVIGNGNNSDSYGSIMKIDEEQVQLMKRRGGVGHDLSYIRPKGSAVK
                     NSALTSTGLVPFMERYSNSTREVAQDGRRGALMLSVSINHPDAEDFINAKLEQGKITG
                     ANISVRIDDEFMQAVKNNTDYKQKYPIHSKHPKFTKTVNAKNIWDKIVHNAWKSAEPG
                     ILFWDTIAKESIPDCYADLGFKTVSTNPCGEIPLCPYDSCRLLAINLYSYVENPFTDK
                     AKFNFELFKKHVALAQRIMDDIIDLELEKVDVILAKINSDPEEEYLKLSERTLWEKIK
                     EKAIQGRRTGVGITAEGDMLAALNLRYGSEAAVDFSIEVHKTLALAAYRSSVEMAKER
                     GAFEVFDAEREKENPFILRLKDADPDLYAQMQLYGRRNIALLTIAPTGTTSLMAQTTS
                     GIEPVFLPVYKRRRKVNPNDKEIRVDFIDEVGDSWEEYIVFHHHFKTWMKVNNIDTTK
                     NYTQEEINELIEKSPYYKSTSNDVDWLSKVKLQGAVQKWVDHSISVTINIPNDATEEL
                     VNELYLKAWEVGCKGVTVYRDGSRSGVLVAADSKKKENTDDNIGGVLTNDFPTKRPQI
                     LEADVVKFQNNKEKWIAFVGLIDEKPYEIFTGLADDDEGISIPRWVQDGHIIKNKDEK
                     GNTRYDFQFKNQRGYKTTIEGLSHKFNPEFWNYAKLISGTLRHGMPLVKVVDLINHIE
                     LDSEFINNWKAGVARALKRYIPDGTEASGHKCSSCGSNEVVYQEGCLTCKSCGSSKCG
                     "
     gene            8149..9408
                     /locus_tag="PLS16_07285"
     CDS             8149..9408
                     /locus_tag="PLS16_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HNJ60922.1"
                     /translation="MKYIFTLIIALVYLTNYAQCDPERYRNEIFPNVITHADIIYGNN
                     KDNKGQSTDLLMTVYEPDEVITELRPLIIFIHGGSFTGGDRTDQHLDFTSERFARKGY
                     VSANIEYRLEQTVFISPYVNFADKNNWYKAMIRSIQDIRASIRYFKKDVAENGNQFNI
                     DTSKIIIYGISAGAIAGLHVAYLDNLEEASAPFKKNFIELGGLEGNSGSAGYTSNGIK
                     AVVNASGAIDNMNYLNNNLDIPLISFHHKIDLTVPFDKGCFITVACFLGDFYGSNKIS
                     QQAKKIGLPYEFYPINKEGHPADDFNDTAVHEMVVAKTTEFLYKHVICTDAMTPIAKR
                     ETKTFNIFPNPSSGWITIENETNLLHQTCTLQIIDQYGQIIQEQEFIADTQNQFSFNI
                     ASGLYTLRVLSNKDLSEQYVSRLAIVH"
     gene            9452..11596
                     /gene="ligA"
                     /locus_tag="PLS16_07290"
     CDS             9452..11596
                     /gene="ligA"
                     /locus_tag="PLS16_07290"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNJ60923.1"
                     /translation="MDLFSHIQPDISTITQTALQKIGQFIPENEVEHYCNDLKSIINY
                     YDKKYYVDADSPISDYDYDILFKQLKELEEAYPNLITADSPTQRVAKGLNETFQTVQH
                     LVPMMSLENSYNIQDLREFERKVKESVATNEKIEYICEPKFDGSSIALVYENNQLVRA
                     ATRGNGIEGDDITPNAKAIKSIPLKANFSQFGIHKIEIRGEVVMQLAVLEQLNKERLE
                     QNIILKNENKKELELFKNARNTAAGSLRLKDSAEVAARKLDAILYQIGYAEDKNGNNI
                     TFSEIFKSHDDNLKLLSDLGFKTPFLDKTLSEDIDQIMATCLDWQDKRDNYPIDIDGM
                     VIKVNNTKLQQIIGKTSHHPKWATAFKFKPKQGFSTLENVEFQVGRTGQITPVAKITP
                     VQLMGVEISSISLHNEDFIIDKDIRLYDTVIVERAGDVIPYIVGSLPEKRNGKETKIE
                     FPKNCPSCAHLLYKNLEESAWRCINTNCPAQLEEHLIHFVSKEAMNIFGFGEENVRTF
                     LKENLISNIASIYKIDYEKVAQLEGWKVKSIQNLKEGIEQSKQNENWRLLVGLGIRHI
                     GTTTAKMLAKQVAHLLDYANWNTEQFMQLEDVGPKVALSLQAFFSDESNIELLKELEV
                     LGLNLKRTEQVLHSEKLAGKTFLFTGTLTQFSRDEAKILVENNGGKNLSAVSANLNYL
                     VAGEKAGSKLTKAQKIESIQIIDEFEFLKMIE"
     gene            11593..11997
                     /locus_tag="PLS16_07295"
     CDS             11593..11997
                     /locus_tag="PLS16_07295"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HNJ60924.1"
                     /translation="MKTSVLDIFIARFYPQTKDKINDLIADSILLKKLTDAAYTKLES
                     SIQYYADISEQIKYVILILNDWANKNYTDINEKKIILGILVLLYFVSPIDLIPDFIPF
                     LGKKDDLFLLKKVSKGLDEEILKYKEWRKTNK"
     gene            complement(11999..13441)
                     /gene="miaB"
                     /locus_tag="PLS16_07300"
     CDS             complement(11999..13441)
                     /gene="miaB"
                     /locus_tag="PLS16_07300"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNJ60925.1"
                     /translation="MEELLDYVKNKQHDESRQGEALNIELEPTDFKNDKYFYIESYGC
                     QMNFSDSEIVASILAKQGYSLTADIFQSDLILLNTCAVRDKAEQTVRKRLQFINELKE
                     QKPGLMIGMLGCMAERLKSKLLEEEKLVDIVVGPDAYRSLPELIAEAEGGQKAVNVLL
                     SREETYADISPVRLDKNGVTAFISIMRGCDNMCSFCVVPFTRGRERSRDPESIVAEAE
                     DLFKRGYKEVTLLGQNVDSYAWRGEGKNDDKEAEVLVNFAQLLEKVALVNPDLRVRFS
                     TSHPKDITDDVLYAIAKYENICKYIHLPVQSGSSSCLKRMNRTYDRDWYINRVDAIKR
                     IIPDCGLSSDMIAGFCGETEAEHQDTLSMMDYVRYDYSYMFYYSERPGTLAARKYPDD
                     VEESVKKRRLQEIIDKQNQLSVENNRKDIGKIYKVLIEKTSKKSTEDFSGRNSQNKMI
                     VFPRGNAKIGDYVNVLVESITQGTLIGKIV"
BASE COUNT         4990 a         2151 c         2050 g         4382 t
ORIGIN      
        1 aggcggtatt ttatttgctt tgggacattt tgtttttatt ccttttttgc gatatgtttc
       61 tacttctggt atgtctgaat tattaaccgc atcttcttta tttttagtta tcggtgtttc
      121 tgaattaatg gttgcagtag gtttatctcc agcattgggt gcttttattg caggtatgat
      181 gttggcaaat agtgaattca gaaatgaatt agaatcgaat attgaaccaa tgaaaggaat
      241 tctcctcgcc gtattttttg tgagcgtcgg tgcaatgatt aatttcaata taatttttga
      301 cgattatatt ttaatattta gcttagttgg attagtatta gtcgtaaaat ttggagtgct
      361 gtgcttgatt ggaaaaatat acaaaataga ttttgttcaa aatttatttt atgcttttac
      421 attatcgcaa attggagaat ttgcatttgt actattagga ttttctaaag aagtatccat
      481 atttaataac caattatatg cacaattgat ggctgttact acaataacca tgctaactac
      541 gcctatctta ttaattatct acgataaatt tattagtcca agtataaaac ccaaaaacac
      601 agatgagtct gattttgata ttcaaaaaat ggatatccaa aagaagaaaa ttattatagt
      661 tggatttggc ttttttggaa gtactgttgg acgattatta aaagcaaata aaatctcagc
      721 aaccatctta gaccatgaca cggagagagt aaaaatatta agagaatatg gatttaaagt
      781 ttattatggc gatgcaactc gttggtctat cttagaaaca tctggcattc acgaagccga
      841 aattcttgtt ctttgtttag ataatccaga agaaaatatg cgaataatag aattagttaa
      901 gaagaaaaaa ctaaatctaa aaatatttgt acgagctaga aatagactag atgcagaact
      961 attttttaaa aatggcatcg ataattttta tcgtgaaacg atgggaaccg cagtaaatat
     1021 ggcggtcgat gttttacatg aaatgggcat gcgtaaatat actgcaagaa gaatgggtaa
     1081 acggtttgaa ttaattgatg aagcttcctt aaagaaaatg gctgaggaaa acaatttaga
     1141 tgctagtttc acaatgaagg aaacaattca aagagagcaa ttattattat ctgaagatag
     1201 tagaaacttt gaatcctatc aacttattga agaaaatcaa gaagatgaaa atgagaactt
     1261 agaggatgaa attacaaatt ccagcaaacc gagcaatgaa gataatgctc aattaaattt
     1321 attctaagct tattttgaaa tattgcttct tcttctaaat gccttaaatc caaaataaat
     1381 taaagcaata aatgtaagca aaaccaaaga ccatttaatt tgtgtcaata cactgccaat
     1441 tttcaataaa gtcaaagaat acattgggaa atgtgttgta ttatagattg agcaaacatt
     1501 ttctccaaaa tcaaccaaaa caattagaat aggtaacaga ttcaatactc gccattttga
     1561 atgagcatat cctcctctat taatcaataa ggtaagcatt gtcaataata aacctccata
     1621 aataaacgga taaatactat cataaatctt tacaaattgc acatattcaa ctctgccatt
     1681 ttcacctaat tggtctaaaa aactatctgc aaatgcttta tcatatccaa atgttaaatc
     1741 aaaaggcatc aaatgttctc catattgttg ttgcatttga gctgtcttat aaggaataat
     1801 tccaaagcca attactgtgt acaaaaacaa cattatccaa agtgattttt gttgaatttt
     1861 ggaataaaag aaatcagaaa gatgtttcat agcatcaaag ttttattgaa tcaatattta
     1921 tctacaaatt ttttatagcc tgaagccaaa tatactaaag cactaattat acaaacaacc
     1981 ataatttttt gagcggtagg atgaaaataa ggattagctc cttgaactaa tgaacgtacc
     2041 gtaaacaaca aaattatata cactactaag agcataccca acaatacaaa aacaaaggta
     2101 taacttttgg gaaaattaat tttataagtt aaaaatgctg ttccaaaagc aacaaacgtc
     2161 attgctacaa atgaaaaatt agctactaaa gcatggacta ataataatct atcataaggc
     2221 gtaatggcag tacttgctaa caaaattcca taagcaaaag ctgaagttcg cactaatttt
     2281 tgaatcgaat aatgattatc tatatgagca actttggaaa taaatgcttt aaaaaaattg
     2341 gctataaacc aagacatttg aaaaactgca atgacaaaga aaaaggtgga aatattttta
     2401 tttccacctg aaaaagtttt atatcttcct aaatcactat agaaattttc caaaaaagaa
     2461 taaccaatac tagaatggtc aaaaaatttt cctccaggat aacataacat cgaaattgtt
     2521 gttaaaacaa caaacgccaa aatagaaaaa atgagtgcat tagctgcact catttcatta
     2581 aatactacct tttttatctt ttccgtctcc accgccaatt gaatcacgca ttttggtatc
     2641 tgcttttaca ttctcaattt tatagtaatc cataatacca agattaccat ttttaaatgc
     2701 ttcagctatt gccattggaa tttctgcttc tgcttcaatt acttttgctt ttgcttcttg
     2761 agcttttgca atcatttcgt gttcttgagc aaccgccatc gctcttcttt cctcagcttt
     2821 tgcttgtgca atattcttat ctgcattcgc ctgatccatt tgtaaaattg caccaatgtt
     2881 ttttcctata tcaatatctg cgatatcaat agataaaatt tcaaatgcag ttccactatc
     2941 caaaccctta tttaatacca cttttgatat agaatctgga ttttccaaca cttgtttatg
     3001 atcagcagaa ctaccaatac tagatacaat tccttcgcca atacgagcca aaacagtctc
     3061 ctctcctgct ccaccgacta attgtctaat attagcacga actgtaactc ttgccttagc
     3121 aattaattgg ataccatctt tcgctacggc agttactggt ggcgattcaa tcactttagg
     3181 atttacagac aattgtactg cttcaaacac atctcgacca gctaaatcaa tagcagttgc
     3241 ttctttaaat gtcaatgaga tatttgcttt gtctgcagaa attaatgcac ttactacttt
     3301 catcacattt ccacctgcta aaaagtgtgc ttctaaacca tctctatcca ctggaatacc
     3361 tgcttttgta gcagtaatca ttgaatttac tatagttgct ggtggcactt ttcgtaattg
     3421 cattacaaaa agttgaatta atccaatatt tacgttagat aatcgtgcag atacccacaa
     3481 actaatatat cgcataaacg taaaaacgaa tattaagata aatagtaaaa gtacaatggt
     3541 aatgactgtt aaaaatgttc ctgacataat gttttctttt aaattggttt tacaaaaatt
     3601 ttattttcct ctattttatg aatttgaatt ttagtttctg ttgtaataaa tcctgttaat
     3661 gcccaaaccg ttactttttg gtcattaaat attgctttac cttctggtct aatatctgta
     3721 actgccacac cttcatcatc tatctttaaa ccaaaatctt caaaattatt aactttccca
     3781 tctattactt ctagtaccga aattttactt ttcgcaattc tttccattcc aaaatataac
     3841 aacaatccat taataataat tgctgccaat acacacaaac ttccgtacaa tgcacctaac
     3901 tcataaaatg ctaaagccaa gccacctaaa gtaactacaa aagctacttt tcctataatt
     3961 aaagtcggca ttacaaaaat ctctactaca aaaagtaatg taccaaatag taaaattcca
     4021 ataacaattg caagaggcga catatctaat tatacctttc aaatataata aatttattcc
     4081 attttcagta ttattgcaat aaaatattta gaaaaatgaa aattgaattt tgggtgattg
     4141 gtaaaaatag cgacaaattc attgatgatg gcatacaagc ttatcttaaa aaaataaaat
     4201 atttcaatga cattcagttt catatctttt ctgatataaa aaacatcaaa gaaactaatg
     4261 tattgaaaga taaggaagct gaaaagtatt tatcgaaaat tgaaccagac gattgcgtta
     4321 tcttattaga cgaaaatgga agcacttata gcagtcgaaa gtttgctcat ttcatcgaac
     4381 aaagacaaaa tcaatctaca agaagatgta tttttatcat cggcggagcg tttggcttta
     4441 atgataagtt atataaaaga agtaattaca aaatctcact ttctgaaatg acattttcgc
     4501 accaattaat tagacttatt tttttagaac aactatacag agcttatact attattaata
     4561 attttcctta tcacaacgat taatttttcg atgttctatt tctatgtttt agcaaataaa
     4621 acccagcaac tgtcattgta atagtaaacg taattctaaa aagcatcgct tttacagttt
     4681 ttatctcata caaaccgccg ttataaatat gtaattgtaa aaacacaaat gcaactatta
     4741 atgcaatgga tgccaataat aaaagtcgca cacctatcca ttgatgcttc caactttcat
     4801 aagctgcaaa taaataaatc cagccacaaa tgaaattagt ataaacaata aatggtacaa
     4861 aattaccttc tttggcacga ataccaaacc aatcaaaaat tactgatgag gtcataaata
     4921 cagaaactac tccaataaaa actaataagg ttggttgaag tattcttatc catttcataa
     4981 gcctaaaaat ctacaacaac aaagctaaca aatattattt aattacaaac ttaacattaa
     5041 gtcatataca cttacttagt tttcaagctt ttgaaattaa aataagtacg aaaatacttt
     5101 tttcataaga accttataca aaataatcaa caataagtca aatttttaac aaatagattt
     5161 caaaatttgc aaacttttta aatgttgata acttttattt tattgattat caatactata
     5221 aatattaatt tttgcacaca tttgaaaatg aaattatttt gttgtagttt tatttcgtta
     5281 aaaatttaca catggaaaca gcaactttag aaccaaaaaa caagaaaatt acttacgaaa
     5341 ctgctaataa tgaagcgtta agatatttta aaggtgatga tttagctgca aaagtttggg
     5401 ttacaaaata tgcattaaaa gattcagatg gcaaccttta tgaagctaat ccagatgata
     5461 tgcattggag aattgccaat gaaattgcac gaattgaaac acgttatcca aatccaatgt
     5521 ctgcacaaga aatctttgat ttaatcaaag attttaaata tatcattcct caaggaagtc
     5581 caatgactgg aattggtaat aattttcaag tggcatcgct ttccaattgt tttgttattg
     5641 gcaatggtaa taacagcgac tcttatggaa gtatcatgaa aattgatgaa gaacaagtac
     5701 aattgatgaa acgtagaggt ggggttggtc atgatttatc ttatatcaga ccaaaaggtt
     5761 cggctgtaaa aaactcagca ttgacctcca caggcttagt gccatttatg gaaaggtatt
     5821 ctaactcaac acgtgaagtg gctcaagatg gcagacgagg tgctttaatg ctttcggttt
     5881 ctattaatca cccagatgct gaagatttta tcaatgcaaa attagagcaa ggaaaaatta
     5941 ctggagcaaa tatctccgta agaattgatg acgagtttat gcaagcagtt aaaaacaata
     6001 ctgattataa acaaaaatat cctatccaca gcaaacatcc taagtttaca aaaacagtca
     6061 atgcaaaaaa tatttgggat aaaatcgttc ataatgcttg gaagtcagca gaaccaggta
     6121 ttttattttg ggatactatt gccaaagaat ccattcctga ttgctatgca gatttaggtt
     6181 ttaaaacagt ttctacaaat ccatgcggag aaattccatt atgtccttat gattcttgca
     6241 gattattagc cataaatctt tattcttatg tagaaaatcc atttacagat aaagctaaat
     6301 tcaattttga attattcaaa aaacatgtgg cacttgctca aagaataatg gatgatatca
     6361 tagatttaga gttagaaaaa gttgatgtaa ttttagctaa aatcaattca gatcctgaag
     6421 aagaatattt aaaattatca gaaagaacgc tttgggaaaa aataaaagaa aaagccattc
     6481 aaggaagaag aactggcgta ggtattactg ccgaaggtga tatgttagcc gctttaaatt
     6541 taagatatgg tagtgaagca gctgtagatt tttctattga agtacacaaa actttagcat
     6601 tggctgctta tcgttcatcg gtggaaatgg caaaagaaag aggtgcattt gaagtatttg
     6661 atgcagaaag agaaaaagag aatccattta ttctacgatt aaaagatgca gatcctgatt
     6721 tgtatgctca aatgcaatta tatggtagac gaaatattgc tttattaact attgcaccaa
     6781 ctggaactac ttctttaatg gcacaaacta cctctggaat agagccagtt ttcctacctg
     6841 tttataagag aagaagaaaa gtaaatccaa atgataaaga gattcgagtt gattttattg
     6901 atgaagtggg cgattcttgg gaagaatata tcgtatttca tcatcatttc aaaacttgga
     6961 tgaaggtaaa taatatagat accactaaaa attatactca agaagaaatc aatgaattga
     7021 tagaaaaatc accttattac aaatctactt ctaatgatgt tgattggttg agcaaagtaa
     7081 aattgcaagg tgctgtacaa aaatgggttg atcactctat ttctgttacc ataaatattc
     7141 cgaatgatgc aacagaagaa ttagtcaatg aattatatct aaaagcttgg gaagtaggct
     7201 gcaaaggcgt tacagtttat agagatggct cccgctcagg cgttttggtc gctgcagata
     7261 gcaagaaaaa agaaaataca gatgacaata taggtggtgt attaacaaac gatttcccaa
     7321 caaaacgtcc acaaatatta gaagctgatg ttgtaaaatt ccaaaacaac aaagaaaaat
     7381 ggattgcatt tgttggatta atagatgaaa aaccttatga aatttttact ggattagcag
     7441 atgatgatga gggtatttca attccacgtt gggtacaaga tggacacatc atcaaaaata
     7501 aagatgaaaa aggtaatact cgttatgact tccaatttaa aaaccaaaga ggttataaaa
     7561 ccaccattga aggtttgtct cataaattta atcctgaatt ttggaactat gccaaattaa
     7621 tttcaggtac acttagacat ggaatgccat tggtaaaagt agtagattta attaaccata
     7681 ttgagttaga ctctgagttt atcaataact ggaaagctgg tgttgctcga gcattaaaaa
     7741 gatacattcc tgatggaaca gaagcgtctg gacataaatg ttcaagttgt ggctctaacg
     7801 aggttgtcta tcaagagggt tgcttgacat gcaaaagttg tggttcaagc aaatgcggct
     7861 aaattttaaa ctagtaaatt atttcataaa aataaaaaat gcccaactcc ttagagttgg
     7921 gcttcataca accgcaaaga aaacagccat aaccagcggt caatactttg acaaagattc
     7981 aactaatatt ccaaatcatc tattttaaac caattattaa attatcaaat ctaacccaaa
     8041 gatttagtca ttttttaaaa taagcttcat tttgttaagt tttgttatta tttttgtaag
     8101 taagtcatta ataataacac tcttatctaa atttttatat agaatattat gaaatatatt
     8161 tttacactca ttattgcttt ggtttatctt actaactatg cacaatgcga cccagaaagg
     8221 tatagaaatg aaatttttcc taatgtcatt acccatgctg atatcattta tggtaataac
     8281 aaagacaaca aaggacaatc tactgattta ttaatgacag tttatgaacc cgatgaagtt
     8341 attactgaat tgcgaccatt gataattttt attcacggag gttcttttac tggtggcgat
     8401 agaaccgacc aacatttgga ctttacttca gaacgttttg caagaaaagg ttacgtttct
     8461 gctaatattg aatatagatt agaacaaact gtttttattt ctccttatgt aaattttgcg
     8521 gataaaaata actggtacaa agctatgatt cgtagcatac aagatattag agccagcatc
     8581 cgttatttta agaaagatgt agcagaaaat ggtaatcaat ttaatataga tacttctaaa
     8641 attattattt atggaatttc tgcaggagca attgctggct tacatgttgc atatttagat
     8701 aatttagaag aagcctctgc tcctttcaaa aagaatttta ttgaattggg tggtttagaa
     8761 ggcaatagcg gaagtgctgg ttatacatca aatgggatta aagcggttgt aaatgcaagt
     8821 ggtgcaattg acaatatgaa ttatctgaac aataacttag atattccgct tatctcattc
     8881 catcataaaa ttgacttaac tgttcctttt gacaaaggtt gttttataac tgtggcctgt
     8941 tttttaggtg atttttatgg tagcaataaa atttctcaac aagctaaaaa aattggtttg
     9001 ccttatgagt tctacccaat taataaagaa ggacatcccg ctgatgattt caatgatact
     9061 gctgtacacg aaatggttgt cgctaaaaca acagagtttt tatacaaaca cgttatttgt
     9121 acagatgcaa tgacgccaat tgcaaaaaga gagacgaaaa cttttaatat ttttccaaac
     9181 ccaagttcag gctggattac aatagaaaat gaaaccaatc tacttcacca aacttgtacc
     9241 ttacaaataa ttgaccagta cggacaaatt atacaagaac aagaatttat tgcggatact
     9301 caaaatcaat tttcattcaa tattgcaagt ggtttataca cgcttagagt actatccaat
     9361 aaagatttat ctgaacaata tgtttcaagg ttagccattg tacattaaaa aattagccat
     9421 tgaatattcc taaattcttg taattttagc tatggattta tttagtcata ttcaacctga
     9481 tattagtaca atcacacaaa ctgcacttca aaaaattgga caatttatac cagaaaatga
     9541 agtagaacat tattgtaatg acttaaaatc aatcattaat tattacgaca aaaaatatta
     9601 tgtcgatgca gattcgccta tcagtgatta tgattatgac atcttattta agcaactcaa
     9661 agaattagaa gaagcatatc caaatttgat tactgctgat tctccaacac aacgtgttgc
     9721 aaaaggatta aatgaaactt ttcaaactgt tcaacatcta gtaccaatga tgagtttgga
     9781 aaattcatat aacattcaag atttaagaga atttgaaaga aaagtaaaag aatcggttgc
     9841 tactaacgaa aaaattgaat atatctgtga gccaaaattt gatggttcat ctattgctct
     9901 agtttatgaa aacaatcaac tagtacgtgc agccacacgt ggaaacggca ttgaaggcga
     9961 tgatatcaca ccaaatgcaa aagcaataaa atcgattcct ttaaaagcaa atttttcaca
    10021 atttggaatt cataaaattg aaattcgtgg agaagtggtg atgcagcttg ctgttttgga
    10081 gcaattgaat aaagaacgct tagaacaaaa tataattcta aaaaatgaaa ataaaaaaga
    10141 attagaacta tttaagaatg caagaaatac cgcagctgga agcttacgct taaaagacag
    10201 tgcagaagtt gctgctcgga aattagatgc tattttgtat caaattgggt atgcggaaga
    10261 caaaaatgga aacaatatta cattttctga aattttcaaa tctcacgatg acaacttaaa
    10321 attattaagt gatttgggct tcaaaacacc ttttttagac aaaacattaa gcgaagatat
    10381 agaccaaatt atggcaactt gcttagattg gcaagataaa agagataatt acccaatcga
    10441 tattgatggc atggtaatca aagtaaacaa taccaaatta caacaaatta tcggcaaaac
    10501 ttctcatcat ccaaaatggg caacagcttt taaatttaag ccaaaacaag gtttttctac
    10561 tttagaaaat gttgaatttc aggtgggcag aactggtcaa attactccag tagcaaaaat
    10621 tacaccagtg caattgatgg gtgttgaaat tagctctatt tctcttcaca atgaagattt
    10681 tattattgat aaagatattc gcctttatga tacagtaatt gtcgaaagag cgggtgatgt
    10741 aattccatat attgttggga gtttgccaga aaaaagaaat ggaaaagaaa caaaaataga
    10801 gtttcctaaa aattgtcctt cgtgtgcaca tcttttgtat aaaaatttag aggaaagtgc
    10861 ttggcgttgt attaatacaa attgtcctgc acaattagaa gaacatttaa tacattttgt
    10921 atccaaagaa gcgatgaata tattcggttt tggagaagag aatgttagaa cttttttaaa
    10981 agaaaatcta atttctaata tcgcttctat ttataaaatt gattatgaaa aagtggcaca
    11041 attagaggga tggaaagtca aatctataca aaatctaaag gaaggcattg agcaatctaa
    11101 acaaaatgaa aattggagat tgttggttgg cttaggtatt cgacatattg gaacaactac
    11161 tgccaaaatg ttggcaaagc aagtggctca tttattagat tatgcaaatt ggaatacaga
    11221 acaatttatg caactagaag atgttggacc taaagttgcc ttatctcttc aagctttttt
    11281 ctctgacgag agtaatattg aattattgaa agaattagaa gtacttggtt taaatttaaa
    11341 acgtacagaa caagtattac attcagaaaa attagctgga aaaacatttt tgtttactgg
    11401 aactttaacg caattttcga gagatgaagc caaaatttta gtagagaata atggaggcaa
    11461 aaatctgagt gctgtaagtg caaacttaaa ttatttagta gctggagaaa aagctggaag
    11521 taaattaacc aaagctcaaa aaattgaaag tattcaaatt attgatgagt ttgaattttt
    11581 aaaaatgatt gaatgaaaac aagcgtttta gatatattta ttgctagatt ttatccgcaa
    11641 acaaaagata aaattaatga tttaattgca gattcaattc tgctgaaaaa actaacagac
    11701 gcagcctaca caaagctaga atcttcgatt caatattatg cagatatctc tgagcaaata
    11761 aaatatgtca tacttattct gaatgattgg gcaaataaaa actatacaga catcaatgaa
    11821 aagaaaatta ttttaggcat tttagtctta ttgtattttg taagtccaat tgatttaata
    11881 cctgatttta ttccttttct aggcaaaaaa gatgatctat ttttactcaa aaaagtgagc
    11941 aaaggtttag atgaagaaat actgaaatac aaggaatgga gaaagacaaa taaatagtct
    12001 aaacaatctt cccaattaaa gtaccttgcg ttatcgattc aactaaaaca ttgacataat
    12061 ctccaatttt tgcattgcct cttggaaaaa ctatcatctt gttttgtgag ttacgaccac
    12121 taaaatcttc ggtacttttc ttagatgtct tttcaattaa aactttatat attttgccaa
    12181 tatcttttcg attattttcg acagataatt gattttgttt atctattatt tcttgtaatc
    12241 ttcttttttt cacggactct tcaacatcat caggatattt ccttgctgct aaagttccag
    12301 gtctttcaga ataataaaac atatagctat aatcgtagcg tacataatcc atcatagata
    12361 aagtatcttg atgttcggct tccgtttctc cacaaaaacc agcaatcata tcagaagata
    12421 acccacaatc aggaattatt ctttttatgg catctactct attgatatac caatctcgat
    12481 cgtaagttcg attcattctt tttaagcaag aactgctacc actctgaact ggcaaatgaa
    12541 tatacttaca aatattttca tattttgcaa tggcatacaa aacatcatct gtaatatctt
    12601 taggatgcga agtggaaaaa cgcacacgta aatcaggatt aaccaaagct actttttcta
    12661 ataattgagc aaaattgact aatacctcag cttctttatc atcattttta ccttcgcctc
    12721 tccaagcata agaatctaca ttttgaccta ataaagttac ttctttataa cctcttttga
    12781 ataaatcttc agcttcagca acgatagact ctgggtctcg acttcgttct ctaccacgag
    12841 taaaaggtac tacacaaaat gaacacatat tatcacaacc acgcataata gaaataaaag
    12901 cagtaacgcc atttttatct aaacgaactg gcgaaatatc agcataagtt tcttctctac
    12961 ttaataaaac attcactgct ttttgtccac cttcagcttc agcaatcaac tcaggcaatg
    13021 agcgataagc atctggtcca acaactatat ctactaattt ttcttcttct agtaacttag
    13081 atttcaaacg ctcagccata caacccaaca ttccaatcat caatcctggt ttttgttctt
    13141 tcaattcatt gataaattgc aaacgttttc ttactgtttg ttcagcttta tctcgaacag
    13201 cacaagtatt tagcaaaatc aaatcggatt gaaaaatatc tgcggtcaat gaataacctt
    13261 gttttgctaa tatggaagca acaatttcac tatcagaaaa attcatttga caaccataac
    13321 tttctatata aaaatactta tcatttttaa aatcagttgg ctctaattct atatttaaag
    13381 cctcgccttg tcgagactca tcgtgttgtt tattttttac ataatctaaa agctcttcca
    13441 taaaatcctt ctattttctg ttgcaaagtt agtaaaatca gtataaactt tcaaatcaac
    13501 aataccaaag caattttagt ctttcaaaaa gcattatatt tgtactaaat tcaatttatt
    13561 tgtgcttaaa tac
//
LOCUS       DAOQCW010000068        13237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1742_length_13237_cov_5.089516, whole genome shotgun sequence.
ACCESSION   DAOQCW010000068 DAOQCW010000000
VERSION     DAOQCW010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13237
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1742_length_13237_cov_5.089516"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PLS16_07305"
     CDS             <1..244
                     /locus_tag="PLS16_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="short chain dehydrogenase"
                     /protein_id="HNJ60926.1"
                     /translation="PRTTIATAAVQNLLGGNSIMNASRKPEILADAAYYILKRDAKTT
                     TGNYFVDDELLASEGITDLSQYAYAPGHDLAPDFFI"
     gene            263..418
                     /locus_tag="PLS16_07310"
     CDS             263..418
                     /locus_tag="PLS16_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA synthetase"
                     /protein_id="HNJ60927.1"
                     /translation="MENLPNEDSNKSKISSYIKKIGVLGFLFFLVKGLIWIAIFVFGV
                     KGCGWVS"
     gene            497..1504
                     /gene="trpS"
                     /locus_tag="PLS16_07315"
     CDS             497..1504
                     /gene="trpS"
                     /locus_tag="PLS16_07315"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNJ60928.1"
                     /translation="MNKERVLSGVRSTGNLHLGNYFGAVRNFVRMQDNYDCYFFIANL
                     HALTTHPDPKQLVASTKAVFAEYLACGLDPEKSVLYLQSDVPEISELYTLLNMLAYKG
                     ELEKCTSFKEKIKKHNENINAGLLTYPVLMAADILIHKAHKVPVGKDQEQHLEMTRNF
                     AVRFNHKYGVDFFPEPQAFNFTEQLVKVPGLDGSGKMGKSEAEGNAIYLCEDEASIRK
                     KVMRAKTDSGPTEPNSSKPIEIENLFALMKLVSTKETIQHFDDAYNNCVIRYGDLKKQ
                     LAEDIIQFVRPFKEKIVEYENKPALLKEVADFGAEKARKSANATISELKTLMGLNNVW
                     I"
     gene            1553..2209
                     /locus_tag="PLS16_07320"
     CDS             1553..2209
                     /locus_tag="PLS16_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNJ60929.1"
                     /translation="MAKKKDSNDNKANIKHIAVAGNIGSGKTTLTSLLAKHYNWNPRF
                     EDVESNPYLNDFYADMPRWSFNLQVYFLNHRLQQLLEIQEGNEVVIQDRTIYEDAHIF
                     APNLHEMGLMNNRDFENYISLFKTINKQITPPNLLIYLKASLGTLVSQIEKRGRAYED
                     NIRLDYLKQLNHYYDNWIENYKEGPLLIIPVDDLNFADKPEDFGKVVDKINAELFGLF
                     "
     gene            2345..3628
                     /gene="eno"
                     /locus_tag="PLS16_07325"
     CDS             2345..3628
                     /gene="eno"
                     /locus_tag="PLS16_07325"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNJ60930.1"
                     /translation="MSYIVSVHARQILDSRGNPTVEVDILTDNGIIGRAAVPSGASTG
                     AREAVELRDGGNLYMGKGVLKAVENVNSTIAEALIGVDVYDQRVIDKIMLELDGTDNK
                     QKLGANAILAVSLAVAKAAAQEAGMPIYRYVGGTNAYTLPIPLMNILNGGAHADNKID
                     FQEFMVMPVGASTFSEGLRMGVEIFHNLKKVLKSKGYSTNVGDEGGFAPEIKSNEEAI
                     ETVLKAIESAGYKPGEQVKIAMDAASSEFYDATKKRYVFKKSDNKEMSSEEMVDYWKT
                     WVNKYPILSIEDGLAEDDWNGWALLTKEIGNKCQLVGDDVFVTNSKILAEGIEKKVAN
                     SILVKVNQIGSLSETIEAVELAHRNRYTSIMSHRSGETEDSIIADLAVALNCGQIKTG
                     SASRSDRMAKYNQLLRIEEELGSQAYYPGKNYRFF"
     gene            complement(3981..5726)
                     /gene="aspS"
                     /locus_tag="PLS16_07330"
     CDS             complement(3981..5726)
                     /gene="aspS"
                     /locus_tag="PLS16_07330"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNJ60931.1"
                     /translation="MYRSHTCGELRITDVNKNVTLSGWVQTSRNFGELLFIDIRDRYG
                     LTQIVFNKTENIDLFTQANQLGREFVVQIKGKVVERSNKNPKNPTGEIEIIANDLNVL
                     SASKLPPFTIENNTDGGDDIRMQYRYLDLRRGEVQEKIIFRHKVGHEVRNYLNANGFL
                     DIETPFLIKSTPEGARDFVVPSRMNPGQFYALPQSPQTFKQLLMVSGFDKYYQITRCF
                     RDEDLRADRQPEFTQIDCEMAFVEQEDILNMFEGLIKHIFKTIKNIDLPDFPRMQYDD
                     AMRLYGSDKPDLRFGMQFNEMNDIVKGKDFKVFDDAELIIGINAENCANFTRKQLDEL
                     TDFVKRPQIGATGLIYLRYNEDGTLKSSVDKFYNEEELKKWAVSAKMKEGDLLLVLCG
                     KAEKVRKQMNELRLELGNKLGLRKADEFKPLWVLDFPLLEYIEEDNRWFAMHHPFTSP
                     KKQDIEFLYTDPGRVRADAYDLAINGVEVGGGSIRIFDKQLQAKMFERLGFSEAEAQK
                     QFGFLMNAFEFGAPPHGGLAFGFDRLCAVLNSVETIRDFIAFPKNNSGRDVMLDSPST
                     IDEKQLKELNIDVKI"
     gene            complement(5787..6992)
                     /locus_tag="PLS16_07335"
     CDS             complement(5787..6992)
                     /locus_tag="PLS16_07335"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNJ60932.1"
                     /translation="MKNEKHIIFILTFMWFCSVVDFMLLMPFGSEFMELYQIQPNKFS
                     MLIAAYSISAGTSALFATFYIDKYDRKKILLIAFFNFAIGSILCSFAHTFELMLFSRF
                     FTGTFGGLLGCTTVAIISDIVPYHRRGKAMGSLNTAFGLASIIGIPLGLFISKYTDEF
                     MPFRIIGFLALFVFVLAYKIIPPLGQYIRETSSLSLKDVIDLFRDKNLRRAYIFVFFL
                     VLGHFMYFSFINPYLQDNLGFEKDETTLMYLIGGISVTITSLSIGKYIDQYGKLKSFI
                     VLLICSFIPVLIIAHTTSLNLLYVLFFCTLLFVFSSGRMIAAMTLLTGAPTEAQRSKF
                     LAIRSAIIEFSEGIAATLGGLILTRNPENGHLEHYGELSYLTVVIGLICIWVVRKIRI
                     NEEINETIA"
     gene            complement(7118..7483)
                     /locus_tag="PLS16_07340"
     CDS             complement(7118..7483)
                     /locus_tag="PLS16_07340"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNJ60933.1"
                     /translation="MNKKFSIIDRIISFKYALQGLKILIKEEHNAWIHLFFMLLAIVF
                     GLIYSISLNEWIVLVLLFALVFLCELFNSAIENLCNYVQPEKHELIGKVKDLASAAVL
                     VSAIAAFIIGLIIFIPKVF"
     gene            complement(7484..8308)
                     /locus_tag="PLS16_07345"
     CDS             complement(7484..8308)
                     /locus_tag="PLS16_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ60934.1"
                     /translation="METISINSLSGRNIQLMIRHPQQQVKGVVLINPGTCIPQKVYWN
                     FADFLTKNNYISITYDYGDAQNFKSKVSHVDWIKDMNAAMQYVVENFADCKKYCVGHS
                     SGGQLIGYSSSAKDFDQLFLVASTNGYWKYLNPLYKLLMLLFWYILVPLNVLINGYFN
                     NKMYGVSGGFPKKIILELRHFCLSKQFFLPYFAQQKVPLFYNTIQCPVKSYYFADDAV
                     TSLKGCQFILDLYANAPREIETLKAKDYGMKYFGHRGFFNKKAEYLLWNKFLIDLI"
     gene            complement(8310..9770)
                     /locus_tag="PLS16_07350"
     CDS             complement(8310..9770)
                     /locus_tag="PLS16_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60935.1"
                     /translation="MKNIFVIFLLFVFSLTNAQDLEWSEDCSKAYHLIFQFKFNESKA
                     LIQTIKSKEPNNKMPYFIENYIDFFTLYVSEDKSLYPQVKNNISNRLVVLKSCDKNSP
                     YYLYTQAEIYLQWALCKLKFQEYLSAVLDIRKAFNLLNENQKKFPNFKANLKSLGFLH
                     TIFGAIPDNFKFGAKVLGLKGSIEQGLKELHEVIELKDFEFKSEALILYTFLQLHINK
                     NQESAWKLLENQKLSTEDNLLNVFIRANVAHYLGKNDEVIQLIQNKPKSDNFYPIYYL
                     DFMLGNALLQKLNKNAYYYLNKYLEKFKGNSYKKEAYRKIAWYYLINNQPSNYKKYLQ
                     LCKNYPIAITDEDKAAQKEAERNLIPNKILLESRILFDGGYFNKSLQKINELDDKQLQ
                     NRDLVEYYYRKARIYDELNKKVEANTNYILVMNKSIGFDYYFAANASLKLAQFFEQNN
                     KKSLAIKYYNKAIALPKDEYENSINAEAKAGLNRVQ"
     gene            9967..10419
                     /locus_tag="PLS16_07355"
     CDS             9967..10419
                     /locus_tag="PLS16_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HNJ60936.1"
                     /translation="MKNVFNQNDTADFISRINKLTPTSKPSWGKMNVEQMLAHCNVTY
                     EMVYDDIHPKPNVFKKFFLKLLVKNIVINEKPYKKNSQTAAEFIIKDSKKFESEKKRL
                     IDYIIKTQQLGKNHFEGKESHSFGVLTSIEWNNMFSKHLDHHLTQFGV"
     gene            complement(10438..11748)
                     /gene="der"
                     /locus_tag="PLS16_07360"
     CDS             complement(10438..11748)
                     /gene="der"
                     /locus_tag="PLS16_07360"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNJ60937.1"
                     /translation="MSYTVAIVGRPNVGKSTLFNRLVGYRKAITDDVSGVTRDRQYGT
                     TEWNGKTFNVVDTGGFVPKTDDIFEKAIKQQVKIAIEEANLIVFMTDVTTSITDLDEA
                     MTRELRKSKKKVLVCVNKVDNSDRYLLANEFYGLGFEDVFMVSSMSGSGTGELLDAIT
                     AEIPDDDIHDDPYADLPKIAIVGQPNAGKSTLLNALVGDERNIVTEIAGTTRDSIHTR
                     YNLFGKEFLLIDTAGIRKKNKVHEDLEFYSVMRAIKAIDEADVCLLLIDAECNIEAQD
                     LKIIKLIENKKKGLVIAVNKWDKIEKDTNTLKEFEKKIKDKIAPFKDVPILFISAQEK
                     QRIHKLVEVLIQVNENRSVRIPTHKLNDILLPEIEKTPPPSYRGDYIRIKYITQLPTK
                     VPTFAFFCNYPEQVKESYRNFLENKLREHFNLTGVPINVFFRKK"
     gene            complement(11829..>13237)
                     /locus_tag="PLS16_07365"
     CDS             complement(11829..>13237)
                     /locus_tag="PLS16_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNJ60938.1"
                     /translation="KIPGEFWKKFGEMGYFGLAYPEEYGGSNLDFFYTVVMIEEVSKV
                     FSGGLAITAAVQTCMSTPYILHHGSEYLKEEFMRPAIAGDKIGCIGITEPGAGSDVQN
                     IQTRATKEGDFYILNGSKTFITNGIYGHFVILVCKTNLNAGAAGVSLLVLDLNSEGIT
                     KNKLKKLGWHASDTAELHFDNVKVPVKNLLGEEGKGFYYLMGGLQLERLAGSIMGYAA
                     CEGMLQYGMEYMQQREAFGRPINKFQVLRHRVAQMAAEIEATKFYVLQTCRMHNEKKY
                     AVKESSISKLLASELADKTAYQVLQFFGGYGYMEEYKIARAFRDSRIGTIGGGTSEIM
                     REIIAKMVIDDANYNRATSSTTSNEEQQKPSTVEEIFTTLPGRLKKEKAAGKNITTLF
                     EFENELFYSVIINDGNITIQKEKTLNTYDLILNTDTETYIAVETGKINPQEAFMTGKI
                     KVSDLMKMMEFGGLFKRL"
BASE COUNT         4677 a         2072 c         1884 g         4604 t
ORIGIN      
        1 gccaagaaca accatagcaa cagcagcggt acaaaattta ttgggaggaa attctattat
       61 gaatgcttct cgaaaaccag aaatattagc ggatgcagct tattatattt tgaaaagaga
      121 tgctaaaact accacaggaa attattttgt agatgatgaa ttgttagcat ctgaaggcat
      181 tacagattta agtcaatatg cttatgctcc gggtcatgat ttagctcctg acttttttat
      241 ataattgcaa ataaaataaa tagtggaaaa tttaccgaat gaagattcaa ataaaagcaa
      301 aatatcaagc tatataaaga aaataggcgt tttaggattc ttattttttt tggtaaaagg
      361 tttaatttgg attgcaatat ttgtttttgg tgtaaaaggt tgtggttggg tttcttaaat
      421 acaattttaa aaaatattct caactgattg tcgtaataat acagctatta cattctaatt
      481 tattattttt gtaaatatga ataaagaaag agttttaagt ggggtacgaa gtactgggaa
      541 tttgcatctt ggaaattatt ttggtgctgt gcgtaatttt gtgcgtatgc aagataatta
      601 cgattgctat ttttttattg ctaatttaca tgcattaaca acacatcctg atcctaaaca
      661 attagtggca tctacaaaag cagtttttgc tgaatattta gcttgtggat tagacccgga
      721 gaaaagtgta ttgtatttac aaagtgatgt gcctgaaatt tctgaattat atacattgtt
      781 gaatatgttg gcttataaag gcgaattgga aaaatgtaca tcattcaaag aaaaaataaa
      841 aaagcataat gaaaatatta atgctggatt attaacttat ccagttttga tggctgcaga
      901 tatattaatt cataaagcac acaaagttcc agtagggaag gatcaagagc aacatttaga
      961 aatgactaga aattttgctg tacgttttaa tcataaatat ggtgttgatt tttttccaga
     1021 accacaagct tttaatttta ctgaacaatt ggttaaggtt ccaggattag atggcagtgg
     1081 aaaaatgggt aaaagcgaag ctgaaggtaa tgctatatat ctttgtgaag atgaggcttc
     1141 tatacgcaaa aaggtaatgc gtgctaaaac cgattcagga cctactgaac cgaattcaag
     1201 caaacctata gaaattgaaa acttatttgc tttaatgaaa ttagtttcta caaaagaaac
     1261 catacaacat tttgatgatg cctataataa ttgtgtcatt cgatatggtg atttaaaaaa
     1321 acaattagca gaagatataa ttcaatttgt tcgtcctttt aaagaaaaaa ttgttgagta
     1381 tgaaaacaaa ccagctttat taaaagaagt agctgatttt ggagctgaaa aagcaagaaa
     1441 aagtgctaat gcaactatta gtgaattaaa aaccttgatg ggattaaata atgtttggat
     1501 ttagtttcag atataatatt ttagatttat ttttaagaac caaataatta atatggcaaa
     1561 gaaaaaagac agcaatgaca acaaggcaaa tatcaaacat attgcagttg cagggaatat
     1621 tggctctgga aaaacaacat taacaagttt gttggcaaaa cactataatt ggaatccaag
     1681 atttgaagat gttgaaagca atccatatct aaatgatttc tatgctgata tgcctcgctg
     1741 gtctttcaat ttgcaagtgt attttttgaa ccatagatta caacaattat tggaaattca
     1801 agaaggaaat gaagtcgtta ttcaagatag aacgatttat gaagatgctc atatttttgc
     1861 tcccaactta catgaaatgg ggttaatgaa caatagagat tttgaaaatt atatttcact
     1921 atttaaaact atcaataaac aaattacacc tccaaattta ttaatttatt taaaagcatc
     1981 tttgggaacg ctagtttcgc agattgaaaa aagaggacgt gcttatgaag ataatattcg
     2041 tttagattat ttaaaacaat tgaatcatta ttatgacaat tggatagaaa attacaaaga
     2101 aggtccttta ttgattattc cagttgatga tttgaatttt gccgataaac ccgaagattt
     2161 tggtaaagtt gtagataaaa tcaatgctga gttgttcggg ttgttttaag cattatatcc
     2221 acattttttt tcaattgttc attttattat ccatagaaaa caatattcta taaataccct
     2281 ttttatttgt tggtaaaaaa cttttatctt cgtgtcatta ttttaaaaca taaataaata
     2341 tactatgagc tatattgtat ctgtacacgc aagacaaatt ttagattcga ggggtaaccc
     2401 aacagttgaa gtagatattt taactgataa cggaattata ggaagagcag ctgttccttc
     2461 tggcgcatca acgggagctc gtgaagcagt tgaacttaga gacggtggaa atttgtatat
     2521 gggaaaaggt gttttgaaag ctgttgagaa tgttaattct acaattgcag aagcattaat
     2581 aggagtggac gtttatgacc aacgtgtgat tgacaaaatc atgttggaat tagatggaac
     2641 agataacaaa caaaaattag gagcgaatgc gatattagct gtttctttgg ctgttgctaa
     2701 agctgctgct caagaagctg gaatgcctat atatcgttat gttggaggaa caaatgctta
     2761 tactttgccc attcctttaa tgaatatatt gaatggtggt gctcacgccg ataataaaat
     2821 tgattttcaa gaatttatgg ttatgccagt tggtgcatct acattttctg aaggtttaag
     2881 aatgggtgta gagatttttc ataatttgaa aaaagtatta aagtcaaaag gttattctac
     2941 aaacgtaggc gacgaaggtg gatttgcacc agaaataaaa tctaatgaag aagccataga
     3001 aacagtctta aaagcgatag aaagtgctgg ttataaacct ggtgaacaag tgaaaattgc
     3061 tatggatgct gcatcttcag agttttatga cgctactaaa aaacgatatg ttttcaaaaa
     3121 atctgataat aaagagatga gttcggagga aatggtagat tattggaaaa cttgggtaaa
     3181 taaatatcca attttatcga tagaagatgg attagccgaa gatgattgga atggttgggc
     3241 attattaact aaagagattg gaaataaatg tcaattggta ggagatgatg tgtttgtaac
     3301 caatagtaag attttagctg aaggtattga aaagaaagta gctaattcaa ttttggttaa
     3361 agtaaatcaa attggaagct tgtcagaaac cattgaagct gtggaattag cacaccgtaa
     3421 tcgatatacc tcaattatga gccatagaag tggcgaaact gaagatagta ttattgctga
     3481 tttagcggtt gctttaaatt gtggacaaat taaaacaggt tcagcatcaa gaagtgatag
     3541 aatggcaaag tacaatcaat tacttagaat tgaagaagaa ttaggtagtc aagcatatta
     3601 tccaggaaaa aattatagat tcttttaatt tgaattttta agttttagaa aaaagctgtc
     3661 cgaaaaggac agcttttttt attgtatgat ttgtattcta gtttctccat ttttatgaat
     3721 acaattagat gattataatt aaatacaatg ctataagttt tcgatttgta ccatataatt
     3781 ccttgattta caataattct atttaacata atataaatta taggacaaaa ttataaaaca
     3841 aaattctgca attaagatga ctgataatac aataaattca aaacctaatt caacgctttt
     3901 gaataattat agtaaaagtt ataacaaaat catttacaga aatgaagaaa aaaaatttgg
     3961 tttttgatta taaaaaaaaa ttaaatcttt acatctatat ttaattcttt cagttgtttt
     4021 tcatcaattg tacttggtga atctaacatt acatcacgac cagaattatt ttttggaaat
     4081 gcaataaaat ctctgatggt ttcaacgctg tttaataccg cacataatct atcaaaacca
     4141 aatgctaatc caccgtgtgg tggtgctcca aattcaaagg cattcattaa gaaaccaaat
     4201 tgtttttgag cttcggcttc actaaaacct aaacgttcaa acatcttggc ttgcaattgt
     4261 ttatcaaaaa ttctaattga accaccgcca acttcaacac cattgatggc caaatcgtaa
     4321 gcatctgcac gaacacgacc tgggtctgta tataaaaact caatatcttg tttttttggc
     4381 gatgtaaacg gatgatgcat tgcaaaccaa cgattatctt cttcaatata ttccaacaac
     4441 ggaaaatcta atacccaaag tggtttaaat tcatctgctt ttcttaatcc taatttgttg
     4501 cctaattcca aacgcaattc attcatttgc ttgcgtactt tttcagcttt accacacaat
     4561 actaataata aatctccttc tttcatcttt gcagatacag cccatttttt tagttcttct
     4621 tcattataaa atttatctac agatgatttt aaagtaccat cttcattata tcttagataa
     4681 attaaacctg ttgcaccaat ttgtggacgt tttacaaaat cggtcaattc atctaattgt
     4741 tttctagtaa aattggcaca attttcagca ttaataccaa tgataagttc tgcatcgtca
     4801 aatactttaa aatcttttcc ttttacaatg tcattcattt cattgaattg cattccaaaa
     4861 cgtaaatctg gtttatcact accatacaaa cgcattgcat catcatattg catacgtgga
     4921 aaatctggta aatcaatatt tttaatggtt ttaaagatgt gttttatcaa accttcaaac
     4981 atatttaaaa tgtcttcttg ctccacaaaa gccatttcac aatctatctg tgtaaactca
     5041 ggttgtctgt ccgctcgtaa atcttcatct ctaaaacatc ttgttatttg ataatattta
     5101 tcaaaacctg aaaccatcaa taattgctta aatgtttgag gcgattgtgg taaagcataa
     5161 aattgaccag gattcattcg acttggtaca acaaaatctc ttgcaccttc tggcgtagat
     5221 tttattaaaa atggtgtttc tatatccaaa aatccattag cattcaagta attacggact
     5281 tcatgaccaa ctttatgacg aaatattatt ttttcttgta cttctcccct tcttaaatcc
     5341 aaatatctgt attgcatacg aatatcatca ccaccatcag tattattttc gatagtaaat
     5401 ggtggtaatt ttgaggcaga taaaacattc aaatcattag cgataatttc aatttctcca
     5461 gtcgggttct taggattttt atttgaacgt tctacaactt tacctttaat ttgaacaaca
     5521 aattcacggc ctaattggtt cgcttgagta aataaatcaa tattttcagt tttattgaat
     5581 actatttgag taagtccata tctatctctt atatctataa ataataactc tccaaaattt
     5641 cttgaagttt gtacccaacc agaaagtgtt acatttttat tgacatctgt aattctcagt
     5701 tctccacaag tatgcgatct atacatagaa taaattttaa tgtttgtagt acaaaaataa
     5761 atgtttaatt gattattttt agtggtttaa gctattgttt cgttaatttc ttcatttatt
     5821 cggattttcc taaccaccca aatacaaatt aacccaataa ccactgtcag ataactcaat
     5881 tctccataat gctccaaatg tccattttct ggatttcgag ttaaaattaa accgcctaaa
     5941 gtagctgcaa ttccttcgct aaattctata attgctgagc gaatggctaa aaattttgag
     6001 cgttgtgcct ctgttggagc acctgttaat aaagtcattg ctgcaatcat tctgcctgaa
     6061 ctaaatacaa acaataaagt acagaaaaac aaaacatata acaaatttaa actggtggta
     6121 tgagcaataa tcagaactgg aataaatgag caaatcaaaa gtactatgaa ggattttaat
     6181 ttaccatatt ggtcaatata tttaccaata gataaagatg taatagttac agaaattcca
     6241 ccaattaaat acattaaagt ggtttcatct ttttcaaatc ccaaattgtc ttgtaaatag
     6301 ggatttataa aggaaaaata cataaaatgt cctaatacta gaaagaaaac aaatatataa
     6361 gctcttctta gattcttatc tctaaacaaa tctataacat ctttcaatga gagcgaagaa
     6421 gtttcacgga tatattgtcc caaaggtgga atgattttgt aagctaaaac aaaaacaaac
     6481 aaagctaaaa aaccaataat tctaaaaggc ataaactcat cggtatattt actaataaac
     6541 aaaccgagtg gtatgccaat aatcgaagcc aatccaaatg cggtattcaa actacccatc
     6601 gccttccctc tcctatgata cggtacaata tctgagatga ttgcgacagt tgtacagcct
     6661 aataaaccac caaatgttcc agtaaaaaaa cgactaaaga gcattaactc aaatgtatgt
     6721 gcaaatgaac ataatatact tccaattgca aaattgaaaa atgcaatgag taaaatcttt
     6781 tttctgtcgt atttatcaat ataaaatgtt gcgaataatg cagatgtccc tgcggaaata
     6841 gaataggctg caattaacat actgaattta ttgggttgaa tttggtacag ttccataaat
     6901 tctgaaccaa atggcatcag taacataaag tccaccacag agcaaaacca cataaatgtc
     6961 aaaataaaaa tgatatgttt ttcatttttc atgctgcaaa attaagtgct acaaatcgct
     7021 caaaatctta attttataaa attaacctaa gaataatttc taaaaattga tatataacaa
     7081 agaattttga tttataaaaa aatataaagt gtaatgttta aaacactttt ggaataaaaa
     7141 ttatcaatcc aataatgaaa gctgcgatag ctgagacaag tacagcagcg gaagctaaat
     7201 cttttacttt gcctatcaat tcatgctttt caggttgtac ataattacat agattttcta
     7261 tggctgaatt aaataattca catagaaata ctaaagcaaa aagcaaaaca agtactatcc
     7321 attcgtttaa cgaaatagaa tatattaatc caaaaacaat ggctaacagc ataaaaaaaa
     7381 gatgaatcca agcattgtgt tcttctttta tcaatatttt caaaccttga agagcatatt
     7441 taaaactgat aattctatct attatggaaa atttcttatt catttatatt aaatctatta
     7501 aaaatttatt ccataataaa tattctgctt tcttattgaa aaatcctcta tgtccaaaat
     7561 atttcattcc ataatcttta gcttttagcg tttcaatttc acgaggtgca ttggcatata
     7621 aatctaaaat aaattgacaa ccttttaatg aagtaacagc atcatctgca aaataatagg
     7681 actttacagg acactgaatt gtattataaa ataatggtac tttttgttga gcaaaataag
     7741 gcaaaaaaaa ttgtttactt aaacaaaaat gccttaattc taaaattatt ttcttgggaa
     7801 aaccaccaga aacgccatac attttattat taaaatagcc atttatcagt acattcagtg
     7861 gcactaaaat ataccaaaac aatagcatta atagtttata tagtggattt agatatttcc
     7921 aataaccatt agttgaagca actaaaaata actgatcaaa atctttagcg gaggagctat
     7981 aacctattaa ttgtccacct gaactatgac ccacacaata ttttttacaa tcagcaaaat
     8041 tttcaacgac atattgcatc gcagcattca tatcttttat ccaatcgaca tgactaactt
     8101 tggatttaaa attctgagca tcaccataat cgtaagtaat tgaaatataa ttatttttag
     8161 ttaaaaaatc tgcaaaattc caatatactt tttgaggaat acatgtacct ggatttatta
     8221 aaacaacacc ttttacttgc tgctgtggat gtcgaatcat taattgaata tttctaccag
     8281 ataatgaatt aatactgatt gtttccatat tattgtactc tatttagacc agctttagct
     8341 tctgcattga tgctgttttc atattcatct ttaggcaaag caattgcttt attataatat
     8401 ttaatagcta aactcttttt gttattctgt tcaaaaaatt gagctaattt taaactggca
     8461 ttggcagcaa aataataatc aaaaccaata cttttattca ttactagaat ataatttgta
     8521 ttggcttcaa cttttttatt taattcatca taaatccttg ctttcctata ataatattcc
     8581 actaaatccc tattttgaag ttgtttgtca tcaagttcat taattttctg aagcgattta
     8641 ttgaaatagc ctccatcaaa taaaattcta gattctaaaa gtattttatt cggtattaaa
     8701 ttacgttctg cttctttttg tgctgcttta tcttcgtctg taatggcaat cggataattt
     8761 ttacataatt gtaagtattt tttataattg ctaggttgat tattgattaa ataataccaa
     8821 gctatttttc gataagcctc ctttttatag gaatttcctt taaatttttc aagatattta
     8881 ttcaaataat agtatgcatt tttattaagt ttctgtaaca atgcatttcc taacataaaa
     8941 tctaaataat aaattggata aaaattatcg gatttaggtt tgttctgtat taattgaatc
     9001 acttcatcat ttttgcctaa ataatgagct acatttgccc gaataaaaac atttaataaa
     9061 ttatcttctg tagatagctt ttgattttct aatagtttcc acgcactttc ttgattttta
     9121 ttgatgtgca attgtaaaaa agtatataaa attagagctt cacttttaaa ctcaaaatct
     9181 ttcaattcaa ttacctcatg aagttccttc agtccttgct caatactacc ttttaaaccc
     9241 aaaacttttg ctccaaactt aaaattatct ggaattgctc caaaaatagt gtgtaaaaaa
     9301 cctaagcttt ttagattagc tttaaaattt ggaaactttt tttgattctc atttaataaa
     9361 ttaaatgctt ttcgaatatc taaaacggca cttaaatatt cttgaaattt tagtttacat
     9421 aatgcccatt gcaaataaat ttctgcttga gtatataaat aatatggtga attcttatca
     9481 caactcttta aaacaaccaa acgattagag atattattct ttacttgagg atataaactc
     9541 ttgtcttcag aaacatacaa cgtaaaaaag tcaatataat tttcaataaa ataaggcatt
     9601 ttattgtttg gctcttttga tttaattgtt tgaattaaag ctttactttc attaaactta
     9661 aattgaaaaa ttaaatgata ggcttttgag caatcctcag accattctaa atcttgagca
     9721 ttcgtcaaag aaaagacaaa taaaaggaat atgacaaaaa tgtttttcaa aacacttgca
     9781 aaataaagta aaaataaaca taattaaaat ccgttcacat ttttatattt tgaattgact
     9841 taaaattaca ttacatattt ataaaaataa aaacatgtaa agtgattttt cgattaaaat
     9901 aaaatgtata tttatcataa gattgaaata gaaaattggg agctttttta aaaacattac
     9961 aatcaaatga aaaacgtatt taatcagaat gatactgcgg actttattag ccgcataaac
    10021 aaattaactc caacttccaa accgagctgg ggaaaaatga atgttgaaca aatgttggca
    10081 cattgtaatg taacttacga aatggtttat gacgatattc atcccaagcc aaatgtgttt
    10141 aaaaaatttt tcttaaaact tcttgtaaaa aatattgtaa ttaatgaaaa accatacaag
    10201 aaaaatagtc aaacagctgc tgagtttatt ataaaagaca gcaaaaagtt tgaatctgag
    10261 aaaaaacgac ttattgacta cattatcaag acacaacaat tgggaaagaa ccattttgaa
    10321 gggaaagagt ctcactcttt tggtgttttg acatcaatag aatggaacaa tatgtttagt
    10381 aaacaccttg accatcattt gactcaattc ggagtttaaa aagttaaata ataaatttta
    10441 cttctttcta aaaaacacat ttataggaac gccagttaaa ttaaaatgtt ctcttaattt
    10501 attttccaaa aagtttctat acgattcttt tacttgctct ggataattgc agaagaaagc
    10561 aaaagttgga actttagttg gtaattgtgt gatatatttt attctgatat aatcaccacg
    10621 atacgatggc ggtggcgttt tttcaatttc aggcaataaa atatcattta atttatgtgt
    10681 tggaattcta acacttcgat tttcatttac ttgaattaat acttccacta atttatgaat
    10741 acgttgtttt tcttgagctg aaataaataa aattggcaca tctttaaaag gagcaatctt
    10801 atctttaatt ttcttctcaa attcttttaa agtatttgta tctttttcaa ttttatccca
    10861 tttattgaca gcaattacca aacctttttt cttgttttct ataagtttga taattttcaa
    10921 atcttgagct tcaatattac attcagcatc tatcaataac aaacacacat cagcttcatc
    10981 aatggcttta atagcacgca ttactgaata aaattctaaa tcttcgtgta ctttattttt
    11041 cttacgaata cctgcagtat ctatcaataa aaactctttt ccaaataaat tatatcgtgt
    11101 gtgtatgcta tctctagtcg ttcctgcaat ttcagtaacg atgttacgct catctcctac
    11161 caaggcattt aataaagtag attttccagc attgggttgt ccaacaatcg caattttagg
    11221 taaatctgca taaggatcat catgaatatc atcgtctgga atttctgctg taatagcatc
    11281 taacaattcg cctgttccac taccagacat agaagaaacc ataaaaacat cttcaaatcc
    11341 taagccataa aattcatttg ctaataaata tctatcgcta ttatccactt tattaacaca
    11401 aactaaaact ttctttttgc tctttctaag ctcacgcgtc atcgcttcat ctaaatcggt
    11461 gatacttgtc gtaacatcgg tcataaaaac aataagattg gcttcttcta ttgcaatttt
    11521 tacttgttgt ttgatggctt tttcaaaaat atcatctgtt ttaggaacaa aacctccagt
    11581 atctactacg ttaaatgttt ttccattcca ctctgttgtt ccatattgtc tatcacgagt
    11641 aacgccactc acgtcatctg taattgcttt acgataacca actaatcgat taaataatgt
    11701 agattttcca acattaggtc ttcctacaat tgctactgta taactcacga tgcaaagata
    11761 gttaaaatac tatattgttg tatgatacag tcttttagat agctttgtta ttatgttatt
    11821 aaactaattt ataaacgttt aaataagcca ccaaattcca tcattttcat taaatcagat
    11881 acttttatct tacctgtcat aaatgcttcc tgtgggttta tttttccagt ttcaactgca
    11941 atatatgttt ctgtgtccgt attcagtatt aagtcgtaag tattaagtgt tttttctttt
    12001 tgaatagtta tattcccatc attaatgata acagaataga acaattcatt ttcaaattca
    12061 aataaggtag ttatgttttt tcctgcagct ttctcctttt ttaaacgtcc tggaagtgta
    12121 gtaaaaattt cttcaactgt tgatggcttt tgttgttctt catttgaagt tgtactagat
    12181 gttgctctat tatagtttgc atcatcaatg accattttgg caataatttc tcgcataatt
    12241 tcagatgtgc cgccaccaat tgttccgata cgtgaatctc tgaatgctct tgcaattttg
    12301 tattcttcca tataaccata tccaccaaaa aattgtaata cttgataagc agttttatct
    12361 gccaattcag atgctaaaag tttggaaatt gaactttctt ttactgcata ttttttttcg
    12421 ttatgcattc tacaagtttg taaaacataa aatttggtcg cttcaatttc tgctgccatt
    12481 tgagctactc gatgacgcaa tacttggaat ttattaattg gtctgccaaa tgcttctctt
    12541 tgttgcatat attccattcc atattgtaac ataccttcac aagcagcata gcccataatc
    12601 gaaccagcta atctttccaa ttgtaaacca cccatcaaat aataaaatcc ttttccctcc
    12661 tctcctaata aattttttac aggaactttt acattatcaa aatgcaattc agcggtatct
    12721 gatgcatgcc aacctagttt tttgagttta tttttggtaa taccttcgct attcaaatct
    12781 aaaactagca aacttacgcc agcagcaccc gcatttaggt ttgttttaca aacgagtatc
    12841 acaaaatgac cgtaaatgcc attagtaata aaggttttgc tgccatttaa aatataaaaa
    12901 tctccttctt ttgttgcacg tgtttgaata ttttgaacat cacttccagc accaggttca
    12961 gtgattccaa tacaaccaat tttatcacca gctattgcag gtcgcataaa ttcttctttc
    13021 aaatattcac ttccatgatg taaaatataa ggagtactca tacaagtttg tactgccgct
    13081 gtaatggcta atcctccaga aaatactttt gaaacttctt ctatcattac aactgtatag
    13141 aaaaaatcta agttgctgcc accatattct tctggataag ctaatccgaa atagcccatt
    13201 tctccgaact ttttccaaaa ttcacctgga attttac
//
LOCUS       DAOQCW010000069        13040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1778_length_13040_cov_5.951714, whole genome shotgun sequence.
ACCESSION   DAOQCW010000069 DAOQCW010000000
VERSION     DAOQCW010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13040
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1778_length_13040_cov_5.951714"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /gene="dcm"
                     /locus_tag="PLS16_07370"
     CDS             complement(<1..743)
                     /gene="dcm"
                     /locus_tag="PLS16_07370"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HNJ60939.1"
                     /translation="MKVVSLFAGCGGLDLGLINAGHKIVFANDFDKDCQITYEKNIGK
                     HFWLGDVKQLDTSILPEYDILTGGFPCQGFSVANLYRDASDKRNELYLQIVRIISETK
                     PKYFLAENVPGLLSLGKGEIAKMILNDFKSIGVADGFSGYDVKMYLLNAANYGVPQGR
                     KRVIFLGVSNEIKKEKREILFQNFPPIKTHDSYDPKLNPFKTLREAIGDLPEPDTKEA
                     EKILNHYGTKHKVKINGYMGNRKLDWDKPG"
     gene            complement(740..1048)
                     /locus_tag="PLS16_07375"
     CDS             complement(740..1048)
                     /locus_tag="PLS16_07375"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNJ60940.1"
                     /translation="MISSFGKKLRSLREHKQLSLREVATEIGIDTSLLGKIERDERQP
                     TKEQIKLVAKFFKFDENKLIREHLSDQVAYKIIEADADIDILKVAEQKIKYLKTKKII
                     "
     gene            complement(1237..2235)
                     /gene="dusB"
                     /locus_tag="PLS16_07380"
     CDS             complement(1237..2235)
                     /gene="dusB"
                     /locus_tag="PLS16_07380"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HNJ60941.1"
                     /translation="MPKIANIDLGEFPIILAPMEDVSDPPFRYVCKQYGADLMYTEFI
                     SSEGLIRNAKKSIQKLDIFDYERPMGIQMFGGEEDAMVQSAKIIEAVQPDLIDINYGC
                     PVNKVVCKMAGAGILQDIPKMIRLTEQVVKATNLPVTVKTRLGWDDNSKNIVEVAERL
                     QDIGIQALSIHGRTRKQMYKGEADWTLIGEVKNNPRMHIPIFGNGDIDSPQKALEYKN
                     KYGVDGIMIGRAAIGSPWIFNQIKAYIFENKLIEQPSIEEKIKTIRTHLQKSVEWKGN
                     VLGILEMRQHYSNYLKGLPNIKPYRAKLVMANEFDEINEILTEIIQEYAGFESVTI"
     gene            complement(2348..2941)
                     /locus_tag="PLS16_07385"
     CDS             complement(2348..2941)
                     /locus_tag="PLS16_07385"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HNJ60942.1"
                     /translation="MNIQDLLSGALGQQAQQVVAQQLGIDQQQAASAIQLALPTLLSS
                     LSHNASKPEGADALFNALGNHNGSTLGNLGGLAQAALASGEGAKILGHIFGNRQGQVE
                     NAISQNSGLGAGQIGKLLQILAPIVMGALGGQKQQQGFDVGGLVSMLAGAATSQKQQN
                     PQASDMLSQLFDQNKDGNVVDDTLKMGAGILGKLFNK"
     gene            complement(3009..4799)
                     /locus_tag="PLS16_07390"
     CDS             complement(3009..4799)
                     /locus_tag="PLS16_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNJ60943.1"
                     /translation="MQKDEEKKISKERFFKAIKIFNYLKPYKWYFGLGLFALVFSSST
                     VMIVPKLIGGLVDVSTGKSFYKIENRNTLGLVFIAVFILQGFLSFLRVYLFARANEPA
                     LANIRKDLYNKIISMPIPFFEERRIGDLTSRITNDIVSLQDTLSLTLAEAFRQVSILV
                     IGTFMLFFTSIKLTLVMLVSVPIVTLLAVVFGKFIQKNSRLTQESLAEANIIAEETMQ
                     NINMVKAYTNEAYESKRYYVKVYDTLQLALKNSLYRGTFISFIIVATFGALMFVLWYG
                     SGMVQAYQLDKTQGISEGDLVSFLIITVFIGAALGGLSDAFGRILKALGASERILEII
                     DLNPETDTKDYTPIKLNGSIHFQDIKFAYPSRKDIEIYKNLNFEVAQGKKIALVGTSG
                     AGKSTVFNLLLSYYKPDNGDIFIDNKNINEITVKQLRGNIAIVPQEVILFGGTIRENI
                     LYGKLDASEDEIIDATKKANAWQFISQFPEGLDTKVGERGIKLSGGQRQRIAIARAIL
                     KDPAILLLDEATSALDAESEVLVQEALNNLMQNRTTLIIAHRLSTIRNVDTIFVLDRG
                     AIVEQGNHNELSLKSDGVYNNLLKLQYQIS"
     gene            complement(4882..5187)
                     /locus_tag="PLS16_07395"
     CDS             complement(4882..5187)
                     /locus_tag="PLS16_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HNJ60944.1"
                     /translation="MTTLLKTEIGRLRIIEFLEGMSLILLYFVAVPLKYMADFPLLVR
                     IIGPIHGALFVAFIIMAYLFSESRNWGWSKLLKVILIASFIPFGIFYTDSKILSKEK"
     gene            complement(5297..5680)
                     /locus_tag="PLS16_07400"
     CDS             complement(5297..5680)
                     /locus_tag="PLS16_07400"
                     /inference="COORDINATES: protein motif:HMM:NF022769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HNJ60945.1"
                     /translation="MEIKGRIIQIMPIVSGEGKNGAWKKQEYILEMPNGSFAPKKVCF
                     SAWSDKIDQFAIKENEEVTVSIDIESREYSGRWYTDVRAWKVSRGQENPAPSAPSYSN
                     STPSTPQSVQEAPVAPSKEDDDLPF"
     gene            5747..6073
                     /locus_tag="PLS16_07405"
     CDS             5747..6073
                     /locus_tag="PLS16_07405"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNJ60946.1"
                     /translation="MFIYLYDRMKQQENSIQRLEKAALILKTVAHPARLAIIDLLKRH
                     QELTVSEIIQAIELEQSLTSHHLTNMKVRGILGSKRVGKQIFYYLKERDVAKVIDCIE
                     HCKPNF"
     gene            6084..6674
                     /locus_tag="PLS16_07410"
     CDS             6084..6674
                     /locus_tag="PLS16_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNJ60947.1"
                     /translation="MSLDKLFWDSLWKNNLTAWDLKKVSPPIKDYFDKIENKDIAILI
                     PGCGNAYEAKYLLELGFTNVTIIDIAPTVVGTLLNELDAYIGKQLSVVCGDFFKHSGQ
                     YDLIVEQTFFCALDKQLRKMYVQKMKSLLKTDGQLVGLLFDIDFGKPHPPFGGNKEEY
                     VALFNQEFAINYMETATNSVKPRLGNELWIELNPLK"
     gene            6681..6932
                     /locus_tag="PLS16_07415"
     CDS             6681..6932
                     /locus_tag="PLS16_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60948.1"
                     /translation="MKDILFKNWHVMRIIRLVLSVILLVEAVQMKEPLLGAFGGIFLL
                     MTLFNAGCGTASCCGTNDNCNTTPAKQNTNKEIEFEEVK"
     gene            6943..7242
                     /gene="trxA"
                     /locus_tag="PLS16_07420"
     CDS             6943..7242
                     /gene="trxA"
                     /locus_tag="PLS16_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNJ60949.1"
                     /translation="MSASFQEIIKSEKPVLVDFFATWCGPCKAMSPILQDLKTQIGDK
                     ATILKIDVDKNPALAQQLSIQGVPTLILYKNGQIVWRKSGVVDKNSLASLINQHS"
     gene            7357..7914
                     /locus_tag="PLS16_07425"
     CDS             7357..7914
                     /locus_tag="PLS16_07425"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNJ60950.1"
                     /translation="MEKLIYEPWPWYLAGVLITVNVYLLLYLGKKFGISTGFKTSCSV
                     LGAGKVASFFNYNWKEERWLLVYILGTIIGGFIAFQFMNGDAPVLVSESTKLALEKLN
                     IVPNQSFLPSEIYGLEFFSIKRLLILVVGGLLIGFGTRWADGCTSGHAISGLSNLQLP
                     SLVAVIGFFIGGLLMTFVFIPLIFK"
     gene            7923..8339
                     /locus_tag="PLS16_07430"
     CDS             7923..8339
                     /locus_tag="PLS16_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNJ60951.1"
                     /translation="MKKDIRFLLVGIFFGIVLTKAQVVSWFRIYEMFRFESFHMYGII
                     GTAVVTGIILVQFIKRTKHKSVDGLEIFIEEKPKTYKANLLGGIIFGLGWALVGACPG
                     PLYILIGGGFFAVIIVILSAIFGTFLYGLLRDKLPH"
     gene            complement(8374..9147)
                     /locus_tag="PLS16_07435"
     CDS             complement(8374..9147)
                     /locus_tag="PLS16_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HNJ60952.1"
                     /translation="MEWDNESLKLFLEEKVAQYNTLDFISDDPIQLPHQFTSKEDIEI
                     IGFLVATIAWGNRKMIIKNGKKMLQLMGNAPYDFILSHNKKQLDRLDTFVHRTFNGVD
                     FKYFIQSLRNIYTKHNGLESIFIQHQSDTSLLPAISAFHSIFFELSHEFRTEKHIANP
                     EKGSVAKRINMFLRWMCRKDNCGVDFGIWAIHPSKLSCPLDVHSGNIARALGLITRKN
                     NDLKALQELDATLRKFDPQDPVKYDFALFGLGVNNELDL"
     gene            9197..9535
                     /locus_tag="PLS16_07440"
     CDS             9197..9535
                     /locus_tag="PLS16_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60953.1"
                     /translation="MSSVFNQELANNTLLLLQDESIVFSTNEEFRNYIIQFINRLITE
                     DFNHLLFILYRIDVHEDKVRTLLADNNGEHAAATITDLIIEREKQKMITRQQFKSQQN
                     IYEDEECELW"
     gene            complement(9543..10784)
                     /gene="clpX"
                     /locus_tag="PLS16_07445"
     CDS             complement(9543..10784)
                     /gene="clpX"
                     /locus_tag="PLS16_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNJ60954.1"
                     /translation="MAKGRAQDFCNFCGKPKSEALILISGLDAHICETCAEQAKIIAD
                     EELVRLKKKPTKRLKISKPKAIKEALDQYIIGQDEAKKAIAVAVYNHYKRLNQKVSDD
                     EVEIEKSNILVVGQTGTGKTLIAKTIAKQLDVPFTIVDATTFTEAGYVGEDVESILSR
                     LLQNCDFDVTQAERGIVYIDEIDKITRKSDNPSITRDVGGEGVQQSLLKLLEGTEVLV
                     PPHGGRKHPEQKLIKVDTKNILFICGGSFDGIEKIIAKRTKAASLGFKKREEHAEIES
                     IKDNFYPFINHQDIKTFGLIPELLGRLPVLVHLNPLDKTALKSILLEPKNALIKQYKK
                     LFAIENIELKINDDAIDLIVEKALEYKLGARGLRTICEIIFNEAMYDLPSDDSIHKFE
                     LTRTYAEEKVNKSTIANLKAV"
     gene            complement(10863..11540)
                     /gene="clpP"
                     /locus_tag="PLS16_07450"
     CDS             complement(10863..11540)
                     /gene="clpP"
                     /locus_tag="PLS16_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNJ60955.1"
                     /translation="MNLGNEFRKYATKHHGLSSVYVDQFIDKNITNLTPNIIEERPMN
                     VAALDVFSRLMMDRIIFMGTAIDDYVSNIIMAQLLFLESADNTRDVQIYINSPGGSVY
                     AGLGIYDTMQYINPDVATICTGMAASMAAVLLCAGEKGKRSALKHSRVMIHQPMGGAQ
                     GQASEIEITYKQIQLLKKELYEIIATHSGQPFEKIEHDSDRDYWMIAEEAKEYGMIDE
                     VLLKNKR"
     gene            complement(11547..12905)
                     /gene="tig"
                     /locus_tag="PLS16_07455"
     CDS             complement(11547..12905)
                     /gene="tig"
                     /locus_tag="PLS16_07455"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNJ60956.1"
                     /translation="MNITKTNTDNVNAVLTVNIQKEDYMPQVDKMLQDYRKKVSIKGF
                     RVGHVPAGLVKKMYGNAILFDEVNKLTSEALNKYILDEKLDILGQPLPKADNNQVVID
                     INQPADITFEFEIGMVPEFSIPELSSIQVNAKKITVTDNDIDKEVNDISLRYGDAEEI
                     TDTIQEKDIITFELKELEGKAIKEGGIQNTTVLNEDMIVDKKVRKALTKQKVGDSFTA
                     NPFTLVDRDSEQVAKNILGIKENIPTILPENFQFTITKVTRLKQSAIDQSLFDKVYGE
                     GNVTSEEDFRNKIRAEIENYTEQSYQNSAKENIYKELMDKIQLDFPEAFLKKFIKNTN
                     EKPVSDEQIDLEYPGFIKGMSWNIISTRIAKDNDIKVEMDDVKAFSKEQIKRQFAMYG
                     GGGLDDATLDMLNDNMLKKEDHVRKSFDAALEQKLFDLLISKVKISEEVVTFEDYVNQ
                     TK"
BASE COUNT         4140 a         2114 c         1952 g         4834 t
ORIGIN      
        1 ccaggtttgt cccaatcaag ctttctgttt cccatatagc cattaatttt aaccttatgc
       61 ttagtgccgt aatgatttaa tattttttcg gcttctttag tgtcaggttc aggtaaatcg
      121 ccaatagcct ctcttaaagt cttgaacggg tttaatttag gatcataact atcatgtgtt
      181 tttataggtg gaaaattttg aaataaaatt tctctttttt cttttttaat ttcgtttgat
      241 acgccaagaa aaataacacg ttttctacct tgaggaacac cgtaatttgc tgcatttaat
      301 aagtacattt taacatcata acctgaaaac ccgtctgcga ctccaatgct tttaaaatca
      361 tttaaaatca ttttggcaat ctcacctttt ccaagactca atagtccagg aacattttcc
      421 gctaaaaaat atttaggttt tgtttctgaa attattctta caatttgtaa ataaagttca
      481 tttcttttat cagaagcatc tctatataga ttagcaactg aaaagccttg acaaggaaac
      541 ccccctgtaa gaatatcata ttcgggtagt atacttgtat ctaattgctt tacatcgcca
      601 agccaaaagt gtttcccaat atttttctca tatgtaattt ggcagtcttt gtcaaaatca
      661 tttgcaaaaa caattttatg cccagcattt attaaaccaa ggtctaaacc accgcaacct
      721 gcgaataatg aaactacctt catattattt tttttgtttt taagtattta attttttgtt
      781 cagcaacttt taaaatatct atatcagcgt ctgcctctat tattttgtat gctacttggt
      841 cgctcaaatg ttctctgatt aatttgtttt catcaaattt gaaaaacttg gcaacaagtt
      901 ttatttgttc tttagtaggt tgtctttcat ctcgttctat ttttccgagc aaagatgtgt
      961 caattccgat ttctgttgca acttcacgta atgacagctg tttatgttcg cgcaatgaac
     1021 gaagtttttt accaaacgaa gaaatcatat tcttagatag tgccatcgag gactaatttt
     1081 gtccaaatat aatattaaat atattcgagg actaattttg accttaaaag ttttcaacag
     1141 tctaagttga ggtatttttc ctgtactgat taaaaaatat atagattaaa ttgttatcga
     1201 cttatattgt aatctattta tacttacatc tagcaattaa atagtaacag attcaaaacc
     1261 tgcgtattct tggataatct ccgttaatat ttcattgatt tcatcaaatt cgttagccat
     1321 tactaatttt gctctataag gttttatatt aggcaatcct tttagataat tgctgtaatg
     1381 ttgtcgcatt tctagaatac ccaaaacatt acctttccat tctacagatt tctgtaagtg
     1441 tgttcgtata gtttttattt tttcctcgat agaaggttgc tcaataagtt tgttttcaaa
     1501 aatataagct ttaatttggt taaaaatcca aggtgagcca attgctgctc tgccaatcat
     1561 aatgccatct acaccatatt tgtttttata ttccaatgcc ttttgtggag aatcaatatc
     1621 gccatttcca aaaattggaa tatgcattcg tggattgttt tttacttctc ctattaaagt
     1681 ccaatcagcc tcgcctttgt acatttgttt tcttgttcta ccatgaatac ttaatgcttg
     1741 gatgccaata tcttgcaatc gctcagcaac ttcaacaatg tttttggaat tatcatccca
     1801 gcctaaacgt gtttttacag taactggtaa gttggtagct ttaacaactt gttctgtcag
     1861 acgaatcatt tttggaatat cttgtaaaat tccagcacca gccattttac aaactacttt
     1921 attaacagga cagccataat ttatatctat taaatcaggt tgcactgctt caataatttt
     1981 agcagattgt accattgcat cttcttcgcc accaaacatt tgaataccca ttggtcgttc
     2041 ataatcaaat atgtctagct tttgaatgct ttttttagca tttcgaatca atccttctga
     2101 tgaaataaac tcagtgtaca ttaaatctgc accatattgt ttgcaaacat atcggaacgg
     2161 aggatcgctc acatcttcca ttggagcgag aattattgga aattcaccta aatcaatatt
     2221 tgcaatcttt ggcattatag gacaaaagta cgattattat tgtaaatact aataagcttc
     2281 ttattaggga aaatgaaaaa gggtgaatag caattactac ccacccttcc aaattaacaa
     2341 ctataaatta tttattaaaa agcttaccta agataccagc acccattttt aaggtatcat
     2401 caactacatt accatcttta ttttggtcaa ataattgaga tagcatatcg ctagcttgag
     2461 ggttttgttg cttttgagaa gttgcggcac ctgccaacat acttactaag ccaccaacat
     2521 caaatccttg ttgttgtttt tgtccaccta aagcacccat tacaatagga gctaaaattt
     2581 gtaagagttt gcctatttgc ccagctccca atcctgaatt ttgagatata gcattttcta
     2641 cttgaccttg tctattgcca aaaatatgac ctaatatttt agcaccttcg ccacttgcta
     2701 aagctgcttg tgctaagcca ccaaggttgc ctaatgttga accattgtga ttgcctaatg
     2761 cattaaataa agcatctgca ccttcaggtt tgcttgcatt atgtgataag gagcttaata
     2821 atgtaggtaa agctaattgt attgcagaag ctgcttgttg ttggtcgatg cctaattgtt
     2881 gtgctacaac ttgttgcgct tgttgtccta aagcaccact taataaatct tgaatattca
     2941 tttttctaat tttagaatat taaaggtata ttatttctat taaaaaaaga aaataagact
     3001 tattaacttt aactaatctg atattgtaat tttagtaggt tgttatatac accatcactt
     3061 tttagtgaaa gctcattgtg attaccttgt tctacgattg cacctctgtc taaaacaaaa
     3121 atagtatcta catttctgat ggttgataat cggtgtgcaa tgattaaagt tgttctgttt
     3181 tgcattaaat tattaagtgc ttcttgtact aatacttctg attctgcatc taaggcactt
     3241 gttgcttcgt ccaataatag aattgctggg tcttttaata tagctcttgc aattgcaatt
     3301 ctttgtctct gtccaccaga taatttaatg cctctttctc caacttttgt atctaatcct
     3361 tctggaaatt gactaataaa ttgccaagca tttgcttttt ttgttgcatc aattatttca
     3421 tcttcagaag catctagttt cccatataaa atattttcac ggatagtacc accaaataat
     3481 attacttctt gaggaactat tgcaatattg cctcttaatt gttttacagt aatttcgtta
     3541 atatttttat tatcaataaa aatatctcca ttatcaggtt tgtaataact caataataaa
     3601 ttaaagacag ttgattttcc agcaccagat gttccaacta atgcaatttt cttcccttgt
     3661 gctacttcaa aatttaggtt tttgtaaatt tctatatctt ttcttgatgg ataagcaaac
     3721 ttaatatctt ggaaatggat acttccgttt aatttaattg gtgtgtaatc tttagtatct
     3781 gtttctggat tcaagtctat tatttctaaa attctttcag atgcaccaag tgcttttaaa
     3841 attcttccaa aagcatcact caaacctccg agtgccgcac caataaatac ggtaattatt
     3901 aaaaaagaaa ctaaatcacc ttctgaaatt ccttgtgttt tgtctagttg ataagcttgt
     3961 accatacctg aaccatacca taaaacaaac attaaagcac caaatgtagc aacaataata
     4021 aatgagataa aagttcctct gtataatgag ttttttaatg ccaattgcaa agtatcataa
     4081 acttttacat aatatctttt gctttcataa gcctcattgg tataggcttt taccatattg
     4141 atgttttgca ttgtttcctc agcaattata tttgcttcag ccaatgattc ttgtgtaagg
     4201 cgagaatttt tttgaataaa ctttccaaaa actacggcta aaagcgttac aattggaaca
     4261 gaaactaaca ttacaagagt taattttata gaggtaaaaa acaacataaa agtaccaatc
     4321 actaaaatgg atacttgtcg aaatgcctca gcaagtgtta gggataaagt atcttgtaat
     4381 gaaacgatat catttgtaat tctactggtt aaatcgccaa ttcttctttc ttcaaaaaat
     4441 ggaatcggca tactgataat cttattgtac aaatcttttc tgatgtttgc taaagcaggt
     4501 tcattagcac gagcaaataa atatactcgc aaaaaagaaa gaaatccttg aagtataaat
     4561 acggcaataa aaactaagcc taaggtattt ctattttcta tcttataaaa actctttcct
     4621 gttgatacat ctactaagcc tccaattaat ttgggtacta tcattacggt tgaactagaa
     4681 aatactaaag caaataatcc taatccaaaa taccatttgt aaggtttaag gtaattgaaa
     4741 atctttatgg ctttaaaaaa gcgttctttt gatatttttt tttcttcgtc cttttgcact
     4801 tttatattgt tgtagctcaa aaatacaagt tgttgttgta ttaattgaat tatattagat
     4861 taattatgaa ttaaggaagg tttatttttc tttacttaaa attttactgt ctgtataaaa
     4921 aataccaaaa ggaataaaac tagcgattaa aattaccttc aacaatttag accaacccca
     4981 atttcttgat tcactaaaaa gataagccat tatgataaaa gctacaaata atgctccgtg
     5041 aataggcccg ataattctaa ctaataatgg aaagtctgcc atgtatttaa gcggtacagc
     5101 tacaaagtat aatagtatta aagacatacc ttctaagaat tcaataattc taagtctgcc
     5161 tatttcagtt tttaatagtg ttgtcatttt ttaattttaa tgaattgtat ttttcaatca
     5221 taaaatgaaa agaccaagtc aaattttggc ttggtcaatt tatttatttt attgttcttt
     5281 agaatatata aatttattag aaaggtaaat catcatcttc ttttgacggt gccacaggtg
     5341 cttcttgaac agactgaggt gttgatggtg ttgaattgga atagcttgga gctgaaggag
     5401 ctggattttc ttgtcctctt gaaactttcc aagctctaac atctgtgtac catctgccac
     5461 tatattctct actttcaata tctatagaaa cagttacctc ttcattttct ttaattgcga
     5521 attggtcaat cttatctgac catgctgaaa aacaaacctt ttttggtgca aaactaccat
     5581 taggcatttc taaaatatat tcttgttttt tccaagcacc attctttcct tcgccactta
     5641 ctattggcat aatttgaatt attcttcctt taatttccat attacaaata tagtgatttg
     5701 gctataataa aaaaattaac ctttcttaat tttgatgtat gaaaatatgt tcatatattt
     5761 gtatgataga atgaagcaac aagaaaattc aatacaaaga ttagaaaagg ctgcactaat
     5821 attaaagaca gttgcacatc ctgctcgatt agcaattata gatttgctaa aaagacatca
     5881 ggaattgact gtttcagaaa ttattcaagc aatagaatta gagcaatctt tgacctctca
     5941 tcatttgact aatatgaagg tgcgaggtat tttgggtagt aaaagagtag ggaagcaaat
     6001 cttttactat ttaaaagaaa gagatgttgc taaagtgatt gattgtattg aacattgtaa
     6061 accaaatttt tagtatgatg gaaatgagtt tagataaatt attctgggat tccttatgga
     6121 aaaataattt aacagcttgg gacttaaaaa aagtatctcc accgataaaa gactattttg
     6181 ataagataga aaataaggat atagcgattc taattccagg ttgtggtaat gcttatgaag
     6241 ctaaatactt attagaatta ggctttacaa atgttactat tatagatatt gcaccaacag
     6301 ttgttggaac tttattaaat gaacttgatg cctatattgg gaagcaatta agtgtagttt
     6361 gtggtgattt ttttaaacat tctggtcagt atgatttaat agtagaacaa actttctttt
     6421 gtgcattaga taagcaactt agaaaaatgt atgttcaaaa aatgaaatct cttttaaaaa
     6481 cagatggtca attggttggc ttactttttg atattgattt tggaaaacca catccacctt
     6541 ttggaggcaa taaagaagaa tatgttgcat tatttaacca agaatttgca attaattata
     6601 tggaaactgc aacaaattct gttaaaccac ggttaggtaa tgagctttgg atagaattaa
     6661 atcctttaaa ataatttgtt atgaaagata tattatttaa aaattggcac gtaatgcgta
     6721 tcattagatt ggtactgtct gtaatattat tagtagaagc agtgcagatg aaagagccat
     6781 tgttaggagc atttggtgga atttttttac tgatgacttt gttcaatgca ggttgtggaa
     6841 cagcatcttg ttgtggaaca aatgacaatt gtaatacaac acctgcaaag caaaatacaa
     6901 ataaggaaat tgaatttgaa gaagttaaat aaaaaaagaa gtatgagtgc atcatttcaa
     6961 gaaattatta aaagtgaaaa gccagtgttg gtagattttt ttgccacttg gtgtggacca
     7021 tgcaaagcaa tgtctccaat tttacaagat ttaaaaactc aaattggtga taaagcaact
     7081 attttaaaaa ttgatgtgga caaaaatcca gctcttgcac aacaattgag tattcaaggt
     7141 gttcccacct tgatattata taaaaacggt cagattgtat ggcggaaaag cggtgtagta
     7201 gataaaaata gtcttgctag tttaatcaat cagcatagct aatacttgaa atatcatatt
     7261 gagtaatagt ttcattactt tttattaaaa ttaagctatt ccttttgttc gggttagtag
     7321 tgctaaattt gtagctttgt ttgaacaatc tactatatgg aaaaattaat ttatgaaccc
     7381 tggccttggt atttagcagg tgtattaatt acagtcaatg tctatttact tttatattta
     7441 ggaaaaaagt ttggtatttc aacaggattt aaaacatctt gtagtgtttt gggtgcagga
     7501 aaagttgctt catttttcaa ttacaattgg aaagaagaaa gatggctttt agtctatatc
     7561 ttaggtacta taattggtgg ttttatagca ttccagttta tgaatggtga tgctccagtg
     7621 ctagtttctg aaagtactaa acttgcatta gaaaagttaa acatagttcc taatcaatct
     7681 tttctgccga gtgaaattta tggcttagag ttttttagta tcaaaagatt attaatattg
     7741 gttgttggtg gtttattgat aggatttgga acacgatggg ctgatggttg cacttcaggt
     7801 catgcaatat caggactttc taatttacaa ttaccttcat tagttgctgt aataggcttt
     7861 tttattggtg gtttattgat gacatttgtt tttattccct taatttttaa ataaacaata
     7921 cgatgaaaaa ggatattaga tttttattag taggtatatt tttcggaatt gttttaacaa
     7981 aagcacaagt tgtttcttgg tttagaattt atgagatgtt tcgatttgaa tcttttcaca
     8041 tgtatggaat tattggaacg gcagtagtta caggtatcat tttggtacag tttattaaga
     8101 gaaccaaaca caaaagtgta gatggtttgg aaattttcat tgaagaaaaa ccaaaaactt
     8161 ataaagcaaa tttattaggt ggaattatat ttggattagg ttgggcatta gtgggtgctt
     8221 gtcctggtcc tctttatatt ctaataggtg gtggtttttt tgcggttata atagttattc
     8281 tttctgcaat ctttggaaca ttcttatatg gcttattaag agataaattg ccgcactaat
     8341 tattagaaga ttataaaatt aagttgttga attttacaaa tccaactcat tatttacacc
     8401 taatccaaaa agggcaaagt catattttac gggatcttgt gggtcaaatt ttcgtaaagt
     8461 agcgtctaat tcttgtaatg ctttaaggtc gttatttttt ctagtgatta atcctaaggc
     8521 tcgtgcaata tttccagaat gtacatctaa tggacaagat aattttgacg gatgaatagc
     8581 ccaaatacca aaatctacac cacaattatc ttttcgacac atccatctta aaaacatatt
     8641 gatacgtttt gctacactgc ctttttcagg atttgcaata tgcttttctg tacgaaattc
     8701 gtgtgacaac tcaaagaaaa tagaatgaaa tgcacttata gctggtaata aactagtgtc
     8761 tgactgatgt tggataaata tactttctaa gccattatgc ttagtgtaaa tatttctaag
     8821 agattgaata aaatatttaa aatctacacc attaaatgtt cgatgcacaa aagtatctaa
     8881 tctatccaat tgttttttat tgtgcgataa gataaaatca tacggagcat tgcccatcaa
     8941 ttggagcatt tttttaccat ttttgataat cattttacgg ttgccccacg caattgtagc
     9001 taccaaaaat ccaatgattt caatatcttc tttacttgta aattgatggg gtagttgtat
     9061 tgggtcatcg ctgataaaat ctaatgtgtt gtattgtgca actttctctt ctaaaaataa
     9121 tttcaagctt tcattatccc actccataaa agaataaaaa taagcattta tgcgaaattt
     9181 gttgtaactt taatctatgt cttcagtatt taatcaagaa ttggcaaata atactttgct
     9241 tttattgcaa gatgaaagta tagttttttc tacgaacgaa gaatttagaa actatattat
     9301 tcaattcatc aatagattga taactgaaga ttttaatcat ctactattta ttttatatag
     9361 aattgatgtg catgaagata aagtgcgaac acttttagca gataataatg gtgaacatgc
     9421 agctgcaaca attaccgatt taattattga aagagaaaaa caaaaaatga ttactcgcca
     9481 acaattcaaa tcccagcaaa atatctatga agatgaagag tgtgaacttt ggtaagttaa
     9541 tactaaactg cttttaaatt tgcaatcgtt gatttattga ctttctcttc tgcataggtt
     9601 ctagttaatt caaatttatg aatactatca tctgaaggta agtcgtacat agcttcgttg
     9661 aaaataattt cacaaatagt acgcaaacct ctggctccca atttgtactc caatgctttt
     9721 tcaacaatta aatctattgc atcgtcattg atttttagtt caatattttc gatagcaaaa
     9781 agctttttat attgttttat cagtgcattt tttggttcta ataatataga ttttaatgca
     9841 gttttatcta aaggatttaa gtgaactaat acaggcaatc ttcctaataa ttcaggaatt
     9901 aaaccaaaag ttttaatatc ctgatgattg ataaatggat aaaaattatc ttttattgat
     9961 tcaatttcag cgtgttcttc tcttttctta aaacccaaag atgcagcttt ggtacgtttt
    10021 gcaatgattt tttcaatgcc atcaaacgaa ccaccacaga taaataaaat atttttagta
    10081 tctactttaa ttaatttctg ttctggatgt ttacgaccac catgtggcgg caccaaaact
    10141 tcagtgcctt ctaataattt caataaagat tgttgcaccc cttcgccacc aacatctcta
    10201 gtaatggaag gattgtcgct ctttctagtt attttatcaa tctcatcaat atatacaatg
    10261 ccacgttcag cttgagttac atcaaaatca caattttgca ataatcttga taaaatactt
    10321 tcaacatctt cgcctacata accagcttca gtaaatgttg tggcatcaac aatagtaaat
    10381 ggaacatcta attgtttagc aattgtttta gcaatcaagg tttttccagt tcctgtttga
    10441 ccaacaacta aaatatttga cttttcaatt tctacttcat catcacttac tttttggttc
    10501 aatcttttat agtgattata aacagcaacc gctatcgctt ttttagcctc atcttgtcca
    10561 ataatatatt ggtctaaagc ctccttaatg gctttaggtt tgcttatttt tagtctttta
    10621 gttggtttct tctttaaacg cactaattct tcatctgcta taatttttgc ttgttctgca
    10681 caggtttcac aaatatgtgc atctaatcca gatattaaaa ttagtgcctc tgatttaggc
    10741 tttccacaaa aattacaaaa atcttgagct cttccttttg ccatttttta gtattcttat
    10801 tagaaaccaa atgcaatctt aaaaagattg catttggaag tattttaaaa tttgaatgat
    10861 atttatcgct tgtttttcaa taaaacttcg tctatcattc cgtattcttt tgcttcttca
    10921 gcaatcatcc aatagtctct atcactatca tgttcaattt tctcaaaagg ctgtccagag
    10981 tgtgttgcaa taatctcgta caattctttt ttcaataatt gaatttgttt gtaagtaatt
    11041 tctatttcag atgcctgacc ttgagcaccg cccattggtt ggtgaatcat tactcttgag
    11101 tgttttagtg cagaacgttt tcctttttct ccagcacata ataaaacagc tgccattgat
    11161 gccgccattc ctgtacagat agttgccaca tcaggattga tatattgcat tgtatcataa
    11221 attcccaaac cagcataaac actgcctcca ggagaattga tataaatttg tacatctcta
    11281 gtgttatcag cagattctaa gaaaagcaat tgagccatga tgatattaga aacataatca
    11341 tcaattgctg ttcccataaa gataattcta tccatcatta atctggaaaa aacatctaaa
    11401 gctgcaacat tcatgggtct ttcctcgata atatttggag taagattagt aatgttctta
    11461 tcaataaatt gatctacata tacacttgat aagccatgat gcttagtagc atattttcta
    11521 aattcgttgc ctaaattcat attttgttat tttgtttgat ttacgtaatc ttcaaatgta
    11581 acgacttctt ctgaaatttt tacttttgat atcaataaat caaacaattt ttgttccaaa
    11641 gctgcatcga atgactttct gacatggtct tcttttttta gcatattgtc attcaacata
    11701 tctagggttg catcatctaa gccaccaccg ccatacattg caaattgtct tttgatttgt
    11761 tctttagaaa atgctttaac atcatccatc tcaaccttaa tatcattgtc ttttgctatt
    11821 cttgtactaa tgatattcca agacatacct tttataaatc ctgggtattc taaatctatt
    11881 tgttcatcag aaactggttt ctcgttggtg ttttttataa actttttcaa gaatgcttca
    11941 ggaaaatcta attgaatttt atccatcaat tctttataga tattttcttt agctgaattt
    12001 tgataggatt gctcagtata gttttcaatc tcagctctga ttttatttct aaagtcttct
    12061 tcagaagtaa cattaccttc gccataaact ttatcaaata aagattggtc tattgctgat
    12121 tgctttaaac gtgtaacttt agtaattgta aattggaagt tttcaggtaa aatagttgga
    12181 atattttctt taatacctaa aatgttttta gcaacttgtt cactgtctct atctaccaaa
    12241 gtaaatggat tagcggtaaa tgaatcgcca actttttgtt ttgttaatgc ttttcttact
    12301 tttttatcta caatcatatc ttcattcaaa acagtagtat tttgaatgcc accttctttt
    12361 attgcttttc cttctaattc tttcaactca aaagtgatga tatctttttc ttggatagta
    12421 tcggtaattt cttcagcatc tccgtatctc aagctgatat cgtttacctc tttatcaata
    12481 tcattatctg taacagttat tttttttgca tttacttgga tagaagataa ttcaggaatt
    12541 gagaactcag gcaccattcc aatttcaaat tcaaaagtaa tatcggcagg ttgattaatg
    12601 tcaataacca cttgattgtt atctgctttt ggaagtggtt gtccaagaat atctaatttc
    12661 tcatctagga tatatttgtt caaagcctca gaagtgagtt tgtttacttc gtcaaaaagg
    12721 atagcattac catacatttt ttttactaat ccagctggca cgtgtccaac tctaaatccc
    12781 ttgatgctta ctttttttct gtaatcttga agcattttgt ccacttgtgg catatagtct
    12841 tccttttgga tattcacagt aagtactgca tttacattgt cggtgtttgt ttttgtaata
    12901 ttcattgaat aaaaaattga tctatctaaa aaaattaaat gttgaacagc agtgcgaaac
    12961 taaccattca acatttgttg ttttggtgcg ggtggaggga ctcgaacccc catggttgcc
    13021 cgctagatcc taagtctagt
//
LOCUS       DAOQCW010000070        12849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1807_length_12849_cov_5.712443, whole genome shotgun sequence.
ACCESSION   DAOQCW010000070 DAOQCW010000000
VERSION     DAOQCW010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12849
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1807_length_12849_cov_5.712443"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PLS16_07460"
     CDS             <1..370
                     /locus_tag="PLS16_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60957.1"
                     /translation="IENPEAEIKKICQHIDINFNPNMLEITQESSSIEPDSEEKGFKK
                     ERVANWQKGGLNNTEIWFCEKIGKQNIQHYGYKFSNVQPNYLLVPLYLISFPIKLILS
                     FMLNLDRMKNIFDTLKRRLS"
     gene            455..1087
                     /locus_tag="PLS16_07465"
     CDS             455..1087
                     /locus_tag="PLS16_07465"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNJ60958.1"
                     /translation="MKVETRKEVANTVTHAIGLIAFVTLIPIMFVKAAKYELDGKLLG
                     LILFSVGLVAVYTSSTVYHAIMHENTKRFLRVLDHISIYFLIAGSYSAFMTMFMPQSI
                     SHPFLYILWGIVAVGIVKKIFFTGRFEILSTSLYVFLGCMGLFLGKYLFQYIPTNVIW
                     MIFLGGASYLFGVIFYAWKGYRYHHAVWHVFVFGGSMLHFLAVFFGLSKM"
     gene            complement(1095..2123)
                     /locus_tag="PLS16_07470"
     CDS             complement(1095..2123)
                     /locus_tag="PLS16_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HNJ60959.1"
                     /translation="MSEKLQYLAIHKVGNKGNNDLLFLTKKRVVLNHEMQLLLTDYFL
                     NAFSSEEYYQFQHDSDLGLNEVYTYASQIFENPKVFQEVSIHIAQHLYNQSLHPKIKS
                     GEFYVVYFKDCFIDGQNVDAIGLFKSENKDTFLEIEQTDEGFDIDSKQGININKIDKG
                     CLIYNIDKENGYTLSIVDNINKSLEAQYWKDDFLNVLVVNNHYHQTNQFLTLTKQFVT
                     KQLEEDFEISKADKIDLLNRSVDYFKKHEQFDKQEFEKEVFHHDNIIQSFREFDQSFK
                     NEFDIEIEDTFFISEQAVKKQARVFKSVIKLDKNFHIYIHGNREMIEQGVDENGRKFY
                     KIYYDNES"
     gene            complement(2133..3269)
                     /gene="nspC"
                     /locus_tag="PLS16_07475"
     CDS             complement(2133..3269)
                     /gene="nspC"
                     /locus_tag="PLS16_07475"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR01047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HNJ60960.1"
                     /translation="MDKKLRIDTAQIPSPAFVLQEDLLIQNLELLKYFQEQADVSIIC
                     ALKGFSFYAEFPLLKKYLKGATASSLFEAQLVYEEMSYKPHTYCPIYVPNDFDTIASI
                     SSHLTFNSLSDWERYKNKIPANVQCGLRVNPGYSEITTDLYNPASPNSRLGIGANLLD
                     KKLPEGISGLHFHALCENNSYTLEKVLNSFSNLYHDLILQCEWLNFGGGHLITHKDYD
                     VEHGIQVLKNFRKKYPHLKEIILEPGSAVGWETGYLVSTVLDIVENGAVKTAMLDVSF
                     TCHMPDCLEMPYQPKVIGSVEQSEYKYQLGGMSCLAGDVMGDWYFEKPLQIGQKIIFN
                     DMIHYTMVKTTTFNGINLPNIGIWRNNEYINLKTFGYEDYKRKL"
     gene            complement(3276..4295)
                     /gene="hemE"
                     /locus_tag="PLS16_07480"
     CDS             complement(3276..4295)
                     /gene="hemE"
                     /locus_tag="PLS16_07480"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HNJ60961.1"
                     /translation="MLKNDLLLRAAKGENVERVPVWMMRQAGRILKEYRAVRSSVDGF
                     KALVKNPELICEVTIQPVDILKVDAAIIFSDILVIPEAMGLNYEMIESKGPLFPETIK
                     SKADIDNLTTDVLDNLNYVFDSLSLTKKTLDNSVPLIGFAGAPFTIFCYMIEGKGSKT
                     FSVAKKFLYTEPKLAHQLLEKITISTIKYLKQQIKCGADIVQVFDSWAGVLGKAQYQE
                     FALPYMKMISDAITEVPVILFAKDAHYIIEDFAKTNCSVIGLDWTIDIANARKQAPTK
                     TLQGNLDPCVLYADEQKIEQEVAKMMQAFGKQQYICNLGHGVYPDVDAEKAKFFVECV
                     KHYKF"
     gene            4297..5079
                     /locus_tag="PLS16_07485"
     CDS             4297..5079
                     /locus_tag="PLS16_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNJ60962.1"
                     /translation="MAKIGFFSVLKNSYISKKIETNMSDGVNPIVEHEKIVNIEHADI
                     YQGKHLVLEDINFDVKKGDFVYLIGRTGQGKSSLLRTLYGDLELKKGEGTVCGYNLKN
                     IKSKEVPYLRRKLGIIFQDFQLLQDKTVYDNLKFVLEAVEINNPKEIKKKIEEALEKV
                     KLPFKIDKYPSQLSGGEQQRVAIARALLNNPELILADEPTGNLDPETTHEVMTLFYNI
                     FQELKTPVIMATHEYQIIEQFPGIIYMCAYNQITKDESHFKK"
     gene            5167..5239
                     /locus_tag="PLS16_07490"
     tRNA            5167..5239
                     /locus_tag="PLS16_07490"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5200..5202,aa:Gly,seq:gcc)
     gene            5298..5380
                     /locus_tag="PLS16_07495"
     tRNA            5298..5380
                     /locus_tag="PLS16_07495"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5331..5333,aa:Leu,seq:taa)
     gene            complement(5454..6110)
                     /locus_tag="PLS16_07500"
     CDS             complement(5454..6110)
                     /locus_tag="PLS16_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ60963.1"
                     /translation="MQTIKIAIIDDHNFLIQSIKDRLSFFDTIEVIHTANNGKQAIDY
                     TQKNSNFDLILMDIDMPEMNGIQATKIIKEQFPHIKILMLTIYDDDENIFQAIQAGAD
                     GYLLKEINPDDLNQAINDTIQGGAAMTPSIALKTLNLLRNPLNAKKEIIEYDIEQLSK
                     REIEILELLAKGFTHTIIAENLFIASSTVRKHIENIYQKLQVHSKVEAVQAAQKRNII
                     "
     gene            complement(6120..7880)
                     /locus_tag="PLS16_07505"
     CDS             complement(6120..7880)
                     /locus_tag="PLS16_07505"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ60964.1"
                     /translation="MNKRILSFLFFAVYFLFQPIILFAQNKDSFETISLAEIQTNSSQ
                     TIALLLKQLHQAETDKNDQQIAKISSQLALTYYYAGDYENNRKYALQAIQFFNQLKDY
                     KSLAYEYGELGFRLKSIDLKSAESYMQKGMSIAQKHNSQKQLISIYNNFGVIKRLLNQ
                     ADSALMYHQKSIAICTKYNDSISLPYSLNNIGTLYLEQKKYELADANFEKALQIRKNL
                     KDAYGISDNYAYSGDLYFAQKNYAKAITAFLHSLKIADSLKISNLILYNYKQLAESYK
                     LNGDTKNALFFADRRQDFGDSLINIQTNDKIIKYQIQFETAEKEKQILQQQLEAKKRK
                     TTILLLTFLAIILIISTYFIYRNLKIKNKQQQQEFELNEARLLIEHQKQLDEQRLTIS
                     RDLHDNIGSQLTFIISSIDTLKYALKITDDKINQRMTSISDFTRTTITDLRDTIWAMN
                     QAEIGFQEMKNRIENFIYKAKNLGQGLAFNFEIDEELNRYTFSSTQGMNIYRIIQEAC
                     NNVIKYANASQINIKILKTKQQIEIAIEDDGIGFDTSKNYNGNGLLNMKKRIQSIEGT
                     LKISSTKGKTQILCTFPIIP"
     gene            complement(8187..8630)
                     /locus_tag="PLS16_07510"
     CDS             complement(8187..8630)
                     /locus_tag="PLS16_07510"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNJ60965.1"
                     /translation="MNTKKTIQKLKKLHAFEVDELINTQHEIAFSHINCLDCANCCKT
                     TPALLQNEDINRIAKHLKISTKQFLQEYTTKDEDNDIVFKKTPCVFLNNDNTCSIYTV
                     RPYACKDYPHTHQKNQLKIIKITENNIPICPAVAEIFKQLEKDLD"
     gene            complement(8651..12193)
                     /gene="smc"
                     /locus_tag="PLS16_07515"
     CDS             complement(8651..12193)
                     /gene="smc"
                     /locus_tag="PLS16_07515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNJ60966.1"
                     /translation="MQLRSIEIKGFKSFADKTIINFQNNITGIVGPNGCGKSNVVDSI
                     RWVLGEQKNKALRLEKMENIIFNGSKDKAASNLAEVSLTLDNTRNLLPTEFQSVTITR
                     SIDRDGDSTYKLNGVNCRLKDIRDLFMDTGISTDSYAIIELKMIDQILNNVDNARRTL
                     LEQAAGISKYKTRKRETILKLNATEADLNRVEDLLFEIEKNLKALENQARKTRQYKSF
                     KEEYKIISIDLSKFELHGIYTKFEHLQKTLQQLEDEKLAIITQIEVNEANLEKEKLSI
                     LDKEKDLSEQQKLLNTFLAEIQENESKKKIDTQKLQFLKDKIKQLHTQIEQASGIINS
                     LEDEISKLTQFNEEDETKLNALKTTFDDAKEKLEAQRNINLEYRTNVETIRNTFNQLQ
                     SNIAQNEKQLAVKENDLNTLERNAQQSLFESEERSKNLTQFQEQINQTEAKINTQKAL
                     IEDLQNKETESEQKIKLLEESLETSRQDLTNKNRTLDAKNNEYKLTKNMIDSLEGFPE
                     SIKFLKKNVNWLKDIPLLSDIIYTDEKYRVAIETVLQPQLNNFIVDTENAALNAIEVL
                     AKSSSGKAGFLILDRFQQLKEQSLTSIPNTIKAAEIVQVDEKYRKLINYLLEGIYIAN
                     ENTNLSEIIKNSKETEPIYIISENGSLMQSKHQISGGAVGLFEGKRLGRIKNLEILDK
                     QIKELDAQVINLKKTFQDTQTEINHLKINSFRNTIQRENNSLQQMEKELVSVKSKLEN
                     EQNNIEKITNNAQVLKEKIEVVKNEIQPLKNNLEQLKKEQEQSKNQLEELEIDYKKAS
                     ETYTQISGEYNNINILYIQQENKIKQNKQTVQFKNNQLSDTKNRLGTAKQEIETTETE
                     VRTLEQNLTQTENSLIASYREKEQKMQDLSTQESDYFDNKEKVQNIEDILRKKNKERQ
                     EKEQNINDNKDTYNDHKLQLNLLKERLSIEFQIDIKDWTENFELPETNKEELEDRIQK
                     VKKKIDNFGEVNPIAEEAYNEMKERYDFIQAQKADLAASKDNLLATMNEIEMTAKDQF
                     METFNAVRLNFIDVFRSMFTPDDNCDMVLQNPDDPLESEIDIIAKPKGKRPQSINQLS
                     GGEKTLTALSLLFGLYLYKPAPFCILDEVDAPLDDNNIKKFNEAIRKFSENSQFIIVT
                     HNKSTMASVDNIYGVTMVKQGISRVVPVDFGSLN"
     gene            12278..12787
                     /locus_tag="PLS16_07520"
     CDS             12278..12787
                     /locus_tag="PLS16_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60967.1"
                     /translation="MEWIYIIGLIGYYLYKAYNKGIENSTKNPKPIKKVEQREPEKSF
                     EDILKEIIQQNEPKVAPKPTPPPKQQQKPVVKSKPLTPNTNKRSEDPILHNHDIPSES
                     IEYDANIENIFHKRYNSIETSSNRIEAKEDENYNVRKSQGFKGLLVDGKLLSAKEMLI
                     AQTVFERKF"
BASE COUNT         4057 a         1887 c         1908 g         4997 t
ORIGIN      
        1 tatagaaaac cctgaagctg aaataaaaaa aatctgccag catattgaca ttaactttaa
       61 tccaaatatg ttggaaataa cacaagaaag ttcatcaata gagccagata gtgaagaaaa
      121 aggttttaaa aaagaacgtg ttgcgaattg gcaaaaaggc gggttaaata atacagaaat
      181 ttggttttgc gaaaaaattg gcaaacaaaa tatccagcat tatgggtata aattttctaa
      241 tgtacaacca aattatcttt tagttccctt gtatttaatt tcatttccta ttaaattgat
      301 tttatcgttt atgttaaatt tagatagaat gaaaaatata tttgatacct taaaaagacg
      361 tttatcttaa ttcaatacta tttttctata tattctttgt tattgctttg tattatagac
      421 tgattatttt aactttagat aaaaattttg ttttatgaaa gttgaaacaa gaaaagaagt
      481 cgctaataca gtaacgcatg caattggttt aatcgcattt gtaacgctta ttcctataat
      541 gtttgttaaa gctgctaaat atgaattaga tggtaaatta ttgggtttaa ttttgttttc
      601 tgttggcttg gtggctgttt atacctcttc aacggtgtat cacgcaatta tgcatgaaaa
      661 cactaaaaga tttttgcggg ttttagatca tattagtatt tattttctaa ttgctggaag
      721 ctattcagca tttatgacga tgtttatgcc ccaaagtatt agtcatccat tcttatatat
      781 tttatgggga atagtggctg ttggcatagt taaaaaaata ttttttacag gtagatttga
      841 aatactatca acaagcttat atgttttcct aggatgtatg ggtttgtttc ttggaaaata
      901 tctttttcag tatataccaa caaatgttat ttggatgata tttttaggcg gtgcatctta
      961 cttgtttggt gttattttct atgcttggaa aggttatcga tatcatcatg cagtttggca
     1021 tgtatttgtt tttggtggta gtatgctgca ctttttagca gttttttttg gactttctaa
     1081 aatgtaattt tggattaact ttcgttatcg taatatattt tatagaattt tctgccgttt
     1141 tcatctacac cttgttctat catttcacga ttgccgtgaa tataaatatg gaagtttttg
     1201 tccaatttga taacactttt aaatactctt gcttgttttt ttacggcttg ttctgaaatg
     1261 aaaaaagtat cctcaatttc tatatcaaat tcatttttga aactttggtc aaattctcgg
     1321 aaggactgaa taatattatc atgatggaaa acctcctttt caaattcttg tttgtcaaac
     1381 tgttcatgtt ttttaaaata atctactgaa cgattgagta aatctatttt gtcagctttg
     1441 cttatttcaa aatcttcttc taattgtttt gttacaaatt gtttggttaa agtcagaaat
     1501 tgattcgttt ggtgataatg attattgacc actaaaacat tcaaaaaatc atctttccaa
     1561 tattgagctt ctaaactttt attgatatta tctacaattg aaagggtata gccattttct
     1621 ttatcaatat tataaatcaa acaaccctta tcaattttat tgatattaat accttgctta
     1681 ctatcaatat caaagccttc atctgtttgt tcaatttcta aaaatgtatc tttattttct
     1741 gatttaaata agccaattgc atctacattt tgtccatcaa taaaacaatc tttaaaataa
     1801 actacataaa attcgccact ttttattttt ggatgtaaac tttggttgta caaatgttgt
     1861 gcaatatgaa tagatacttc ttgaaaaact ttaggatttt caaaaatctg tgaggcataa
     1921 gtatatactt cattcaaacc taaatcactg tcatgttgaa attgataata ctcttccgaa
     1981 gaaaatgcat ttagaaaata atccgtcagc aataattgca tttcgtgatt taagacgacc
     2041 cttttctttg ttaaaaaaag caagtcatta ttgcctttat tacccacttt atgtatggct
     2101 aaatattgta gtttctcact catattgatt gcttaaagct ttcttttata atcttcgtaa
     2161 ccgaaagttt tcagattgat gtattcatta tttcgccaaa taccaatatt gggcaaattg
     2221 ataccattaa aagtagtagt ctttaccatt gtataatgta tcatatcatt aaaaataatc
     2281 ttttgaccta tttgtaatgg tttttcaaaa taccaatcgc ccataacatc gccagctaaa
     2341 caactcatgc cacctaattg atatttatac tcactttgtt ctacacttcc aataactttt
     2401 ggttgataag gcatttctaa acaatctggc atgtggcaag taaaagaaac atctaacata
     2461 gcagttttta cagctccatt ttctacaata tccaatactg tcgaaactaa ataaccagtt
     2521 tcccaaccaa ctgcactacc tggttctaaa ataatttctt ttaaatgagg atattttttt
     2581 ctaaaatttt tcaaaacttg aatgccatgt tctacatcat aatctttgtg tgtaatcaaa
     2641 tgtccaccac caaaatttag ccattcgcat tgtaaaatta aatcgtggta tagattagaa
     2701 aatgaattta aaactttttc taaagtataa gagttgttct cacacaaagc atgaaaatgt
     2761 aaaccagaaa ttccctctgg taattttttg tccagtaaat tagcaccaat tcctaatctt
     2821 gagtttggtg atgctggatt atataaatca gtagtaattt cgctatagcc aggatttaca
     2881 cgcaagccac attgaacatt agcaggaatt ttatttttat atctttccca atcacttaat
     2941 gaattgaaag taagatgaga agatattgaa gctattgtat caaaatcatt aggtacataa
     3001 ataggacagt aggtgtgtgg tttatagctc atttcttcat atactaattg agcttcaaat
     3061 aaagaacttg cagtagctcc tttaaggtat tttttcaata aaggaaattc agcatagaaa
     3121 gaaaaacctt ttaaggcaca aataatagaa acatcagctt gttcttgaaa atattttaat
     3181 aactctaaat tttgaatcaa taaatcttct tgtaaaacaa aagcaggtga tgggatttga
     3241 gctgtatcta tgcgtagttt tttatccatt attaattaga atttataatg cttcacacat
     3301 tcaacgaaaa actttgcctt ttctgcatca acatcaggat agacaccatg ccctaaattg
     3361 caaatgtatt gttgtttgcc aaaagcctgc atcattttgg ctacttcttg ttcaattttt
     3421 tgttcatcag catataaaac acaaggatct aaattgcctt gaagtgtttt tgtaggagct
     3481 tgttttcgtg cattagcaat atcaatagtc caatctaaac caattacact acaatttgtt
     3541 tttgcaaaat cttcaatgat gtaatgcgca tcttttgcaa ataaaataac tggtacttct
     3601 gtaattgcat cgctaatcat tttcatataa ggtaatgcaa attcttggta ttgagctttg
     3661 cccaaaacac cagcccaact atcaaaaact tgcacaatat ctgcaccaca ttttatttgt
     3721 tgctttaaat atttgatggt agaaatggta atcttttcta ataattgatg tgctaatttg
     3781 ggttcggtat ataagaattt tttagctaca gaaaatgttt tgctgccttt tccttctatc
     3841 atataacaaa aaatagtaaa tggagcaccc gcaaaaccaa ttaaaggaac gctgttatcc
     3901 aaggtttttt tagttaaact aagagaatca aaaacataat tcaaattatc taaaacatca
     3961 gtcgttaagt tatctatatc tgcttttgat ttaatggttt caggaaataa aggtcctttg
     4021 ctttcaatca tttcataatt caagcccatt gcttctggaa tcactaatat atcagaaaaa
     4081 ataattgctg catcaacttt taaaatatct acaggctgaa ttgttacctc acaaatgagt
     4141 tcaggatttt ttactaatgc tttaaatcca tcaacactac ttcttactgc tctatattct
     4201 tttaaaattc ttcctgcttg tcgcatcatc caaactggca ctctttcaac gttttcgcct
     4261 tttgcggctc tgagtagtaa atcatttttc aacataatgg caaaaatagg cttttttagt
     4321 gtcttaaaaa atagttacat tagcaaaaaa atagaaacaa atatgagcga tggtgttaat
     4381 ccaattgttg aacatgaaaa aattgtaaat atagaacacg ccgatattta tcaaggtaag
     4441 catttagtat tagaagatat aaattttgat gtaaagaaag gcgattttgt ttacttgatt
     4501 ggaagaactg gtcaaggtaa aagtagttta ttgcgtacat tatatggtga tttggaattg
     4561 aagaaaggag aggggacagt ttgtggttat aatttaaaaa atatcaaatc gaaagaagtg
     4621 ccttatttaa gaagaaaatt aggtattatt ttccaagatt ttcagctatt acaagataaa
     4681 acagtgtatg ataatttgaa atttgtttta gaagctgttg aaattaataa tcctaaagaa
     4741 ataaagaaaa aaatagaaga agcattagag aaagtgaaat tgccctttaa aatcgataaa
     4801 tatccatctc aactttctgg aggagagcaa caacgtgttg caattgcaag agctttattg
     4861 aataatccgg aattgatttt ggcagatgag cctactggaa acttagaccc tgaaacaaca
     4921 cacgaagtaa tgactttgtt ttataatatt tttcaagaat tgaaaacacc agtaattatg
     4981 gcaacgcatg agtatcaaat cattgaacag tttccaggta ttatttatat gtgtgcttat
     5041 aatcagatta ccaaagacga aagccatttt aaaaaataat ttcaaaaaaa aagttgaaaa
     5101 aagtgctgaa aagattttga attaaaaaaa aatgcttatc tttgcacgca gttctttaaa
     5161 aacaatgcga aagtagctca gttggtagag catcaccttg ccaaggtgag ggtcgcgggt
     5221 tcgagtctcg tctttcgctc tgattttttc acacataaat ttgtcataaa taagtgtgga
     5281 atataaatac taaagatgcc caggtggtgg aatggtagac acgcaggact taaaatcctg
     5341 tggcttcacg gctgtgcggg ttcgagtccc gccctgggta cttctttatt ttcttcttta
     5401 aattattatc aatcttcttc cttttcaacc aaaattattc taagtgtatt caattagata
     5461 atatttcgtt tttgagctgc ttgtacagct tcaactttag aatgtacttg tagtttttgg
     5521 tagatatttt caatatgttt tcgcacagtt gatgatgcaa taaaaagatt ttcagcgatt
     5581 atggtatgtg taaatccctt tgccaatagt tctaaaattt caatttctct tttggataat
     5641 tgttcgatat cgtactctat aatttctttt ttagcattaa gcggatttct tagtaaattc
     5701 aaggttttta gagcaattga tggtgtcatt gcagcaccac cttgtatagt gtcatttatg
     5761 gcttgattca aatcatcggg attgatttct tttaataaat atccatcagc tcctgcttga
     5821 atggcttgaa atatattctc gtcatcatca taaattgtca gcattaaaat cttgatgtgt
     5881 ggaaactgtt cttttataat ttttgttgct tgaatgccat tcatttctgg catgtcaata
     5941 tccattaaaa ttaaatcaaa attagaattc ttttgtgtat aatctatagc ctgtttgcca
     6001 ttatttgcgg tgtgaatgac ttcaatagta tcaaaaaatg agagtctgtc tttaattgat
     6061 tggattaaga aattatggtc atcaataatt gcaattttta tcgtctgcat tgcttgaaat
     6121 taaggaataa ttggaaaagt acaaaggatt tgtgttttac cttttgtaga agaaatttta
     6181 agtgtgcctt caatagattg gatacgtttt ttcatattca ataatccatt tccattgtaa
     6241 ttttttgaag tatcaaagcc aattccatca tcttctatgg ctatttcaat ttgttgctta
     6301 gtttttagaa ttttgatatt tatttgactt gcatttgcgt attttatgac attattacaa
     6361 gcttcttgaa tgattctata aatattcatg ccttgtgtag atgaaaaagt ataacgattt
     6421 aattcttcat caatttcaaa attaaatgcc aacccttgac ccaaattttt agctttataa
     6481 atgaaatttt caattcgatt tttcatttcc tgaaatccta tttctgcttg attcattgcc
     6541 caaattgtat ctctcaagtc ggttattgtt gttcttgtaa aatctgaaat agaggtcatt
     6601 ctttggttga ttttatcatc agtaattttt aaagcatatt tcaacgtatc tattgatgag
     6661 atgataaaag ttaattgaga gccaatatta tcatgtaaat ctcttgaaat ggtcaaacgt
     6721 tgttcatcta attgcttttg atgttcgatt aataatcttg cttcattaag ttcaaattct
     6781 tgttgttgtt gtttgttttt aatttttaaa tttcgatata tgaaataagt agaaataatt
     6841 aatataatcg ccaaaaatgt aagtaaaagt atggttgttt ttcttttttt tgcttctaat
     6901 tgttgttgta aaatttgttt ttctttttca gctgtttcaa attgaatttg gtattttata
     6961 attttatcat tggtttgaat attaatgaga ctatcgccaa aatcttgcct tctatctgca
     7021 aaaaataatg catttttagt atcaccattc aatttatagc tttctgcaag ttgcttgtaa
     7081 ttgtataaaa ttaaattgct aatctttaag ctgtcagcaa tttttaagga atgcaaaaat
     7141 gctgtaattg ctttagcata atttttttgt gcaaaatata aatcacctga ataggcataa
     7201 ttgtcagaaa tgccataagc gtcttttaag tttttacgaa tttgcaatgc cttctcaaaa
     7261 ttagcgtctg caagttcata tttcttttgt tcaagatata gcgttccgat attatttagg
     7321 ctataaggca aactgatact gtcattgtat ttggtacaaa tagctatgct tttttgatga
     7381 tacattaagg cactgtccgc ctgatttagt aatcgtttta ttacaccaaa attattgtat
     7441 atactgataa gttgtttttg tgagttgtgt ttttgagcaa tggacattcc tttttgcata
     7501 taagattcag cactttttaa gtctatactt ttaagtcgaa aacccaattc tccatattca
     7561 taagccaaag atttataatc ttttaattga ttgaaaaatt gtatggcttg taaagcatat
     7621 tttctgttgt tttcataatc tccagcataa taataggtca atgccaattg acttgaaatt
     7681 tttgctattt gttggtcgtt tttatctgtt tctgcttgat gtaattgttt taaaagtagt
     7741 gcaatagttt ggcttgagtt agtttgaatt tcagcaagtg aaatcgtttc gaaggaatcc
     7801 ttattttgag cgaataaaat aataggttga aataggaaat aaacagcaaa aaataaaaag
     7861 gataaaatgc gtttattcat aatggatact tattcgcttt tgcttcttta tcgtgaaaat
     7921 atactttttt attgaaaaaa tgaaatgcca acaatataaa ttagcataga gtttgtatta
     7981 aaaatacgtc atttgacgta ttgtaggctt gatattagac aagtaaattt gcattacaaa
     8041 aacttcattt ataaaacaaa gaccataata atgtgataaa caaacatctt gcaatgtgtt
     8101 taattagttg ggtaaaactc cttttttaaa aggagttttt tttgttttga tgatgaaatg
     8161 aatggtttgt gaagtttctt attaaactaa tccaaatctt tttctaattg cttaaaaatt
     8221 tcggctactg ctggacaaat cggaatattg ttttctgtta ttttaatgat ttttaattga
     8281 tttttttggt gtgtgtgagg atagtcttta caagcataag gacgcacagt ataaattgaa
     8341 caagtattgt cattgttaag aaaaacacaa ggcgtttttt tgaaaacaat atcgttatcc
     8401 tcatcttttg tagtatattc ttgtaggaat tgtttggtgc taatttttaa atgtttagcg
     8461 attcgattaa tgtcttcatt ttgcaaaagg gcaggagttg ttttgcaaca atttgcacaa
     8521 tctaaacaat tgatatgact aaaagcaatt tcgtgttggg tgtttatcaa ttcatcaact
     8581 tcaaaagcat ggagtttttt gagtttctgt atagtctttt tagtattcat catttatatg
     8641 gaatactgtg ttaatttaaa cttccaaaat caacaggcac aactctagaa atgccttgtt
     8701 ttaccatagt tacgccgtaa atattatcaa ccgaagccat agtacttttg ttgtgagtaa
     8761 caattataaa ttgtgaattt tctgagaatt tacgaattgc ttcattgaat tttttgatat
     8821 tattatcgtc caaaggagca tcaacctcat ctaaaataca gaaaggtgca ggtttatata
     8881 aatataaacc aaataataaa gacaatgcag ttagtgtttt ttcgccacca gaaagctgat
     8941 tgatggattg tggacgtttt ccttttggct ttgcaattat atcaatttca gactccaatg
     9001 gatcgtcagg attttgtaat accatatcac aattgtcatc tggtgtgaac atacttctga
     9061 aaacatcaat aaaattaagc cttacagcgt taaatgtttc cataaattgg tcttttgcag
     9121 tcatttcaat ttcattcata gttgccaata aattatcttt tgaagctgct aaatctgctt
     9181 tttgtgcttg tataaaatcg taacgttctt tcatttcatt gtaagcctct tccgctattg
     9241 gatttacttc tccgaaattg tcaattttct tctttacttt ttgtattcta tcttctaatt
     9301 cttccttatt tgtttcaggc aactcaaaat tttcagtcca atctttgatg tcaatttgaa
     9361 actcaatact caatctttct ttcaaaagat ttaactgtaa tttgtggtca ttgtaagtat
     9421 ctttattatc gttgatgttt tgttcttttt cttgtctttc tttatttttc ttccgtaaaa
     9481 tatcttcaat attttgcact ttttctttat tgtcaaaata gtcagattct tgcgttgaaa
     9541 gatcctgcat tttttgttct ttctctcgat aagatgcgat taagctattt tcagtttgag
     9601 ttaaattttg ttctaaagtg cgtacttcag tttcagtggt ttcaatttct tgtttggcag
     9661 tccctaatct attttttgta tctgaaagtt gattgttttt aaattgaacg gtttgtttat
     9721 tttgtttgat tttattttct tgttgaatat agagaatatt gatattgtta tattctcctg
     9781 atatttgagt ataagtttca cttgcctttt tataatcaat ttctaattct tcaagctgat
     9841 ttttggattg ttcttgttct ttttttagct gttctaaatt attttttaaa ggttgaattt
     9901 cattttttac aacctcaatt ttttctttta aaacttgtgc attattggtg attttttcaa
     9961 tattgttttg ctcattttcc aatttagatt ttacacttac caattccttt tccatttgct
    10021 gcaagctgtt attttcacgc tgaatggtat ttctaaaaga atttatttta aggtgattaa
    10081 tttctgtttg tgtatcttga aatgttttct ttagattgat aacttgtgca tctagttctt
    10141 tgatttgctt gtctaaaatc tctaaatttt taattctacc caaacgttta ccttcaaaca
    10201 agccaactgc tccacctgaa atttgatgtt ttgattgcat caaactgcca ttttctgaaa
    10261 ttatatagat tggttctgtt tctttagagt ttttaatgat ttcagataga ttagtgtttt
    10321 catttgctat ataaatccct tctaataaat aattaataag ctttctgtat ttttcatcaa
    10381 cttgtacaat ttctgctgct ttaatagtat taggaataga agtcagcgat tgttctttaa
    10441 gttgttggaa tctatctaaa attaaaaaac cagctttgcc tgaactactt tttgccaata
    10501 cttcaatcgc atttaaagca gcattttcgg tatctacaat aaaattattg agctgtggtt
    10561 gtaaaacagt ttcgatagct actcgatatt tttcatcagt ataaataata tctgaaagca
    10621 aaggaatatc tttcaaccaa ttgacatttt tctttaaaaa tttaatagac tcaggaaaac
    10681 cttccaaact gtctatcata tttttagtta gtttgtattc gttgttttta gcatctaaag
    10741 ttctgttttt attggtcaaa tcttgtcttg aagtttctaa actttcttcc aataacttaa
    10801 tcttttgttc gctttcagtt tctttgtttt gtaaatcttc aataagagcc ttttgagtgt
    10861 ttattttagc ttctgtttga ttgatttgtt cttgaaattg cgtaagattt ttacttcgtt
    10921 cttcactttc aaataaagat tgctgtgcat tgcgttctaa agtgtttaaa tcgttttctt
    10981 ttaccgccaa ttgcttttca ttttgtgcaa tattactttg taattgattg aaagtatttc
    11041 tgatagtttc gacattcgta cgatattcta agttgatatt gcgttgagcc tctaattttt
    11101 ccttcgcatc atcgaaagtt gtttttaagg catttaattt agtttcatct tcttcattga
    11161 attgggtgag tttagaaatt tcatcttcca aactatttat aatgcctgaa gcttgttcaa
    11221 tttgcgtatg tagttgcttg attttgtctt ttaaaaattg taatttttga gtatcaattt
    11281 ttttcttgct ttcattttct tgaatttctg ccaagaatgt attcagtaat ttttgttgtt
    11341 ctgataaatc tttttcttta tctaaaatac tcaatttttc tttttctaaa ttagcttcat
    11401 tcacttcaat ttgtgtgatg atagccaatt tttcatcttc taattgttgt aaagtttttt
    11461 gtaaatgttc aaacttggta taaattccat gtaattcaaa tttggataaa tctatcgaaa
    11521 tgattttgta ttcttcttta aaagatttgt attgtcgggt ttttcgagct tgattttcta
    11581 atgcttttaa gtttttttca atttcaaaca ataaatcttc tacacgattc aaatcggctt
    11641 cggtagcgtt tagttttaaa atggtttctc gttttcttgt tttgtattta gaaattcctg
    11701 cggcttgttc caataaagtg cgacgagcat tgtccacgtt atttagaatt tggtctatca
    11761 tttttaactc gataatcgca taagaatccg tactaattcc agtatccata aataaatcac
    11821 ggatatcttt taatctacaa tttacgccat ttagtttgta ggtgctatcg ccatctctgt
    11881 caatagaacg tgtgatggta acgctttgaa attcggtagg taataaattt cgtgtattgt
    11941 ctaatgtaag tgaaacttca gctaggttgc tagcagcttt atccttagag ccattaaaaa
    12001 taatattttc cattttctcc aaacgtagag ctttattttt ttgctcaccc aaaacccaac
    12061 ggatagaatc aaccacgttt gatttaccac aaccgtttgg accaactatt cctgtaatat
    12121 tattttggaa attgataatc gtcttgtcag caaaactttt aaatccttta atctcaatac
    12181 ttcttaattg catagcctac aaatatacca aaaattaaaa ataaatatgt gctataatat
    12241 tttgtagaat attaaaattt tgctatattt gaattgtatg gaatggattt atattatcgg
    12301 tctaatagga tattatttat ataaagcgta taacaaaggt attgagaact caacgaagaa
    12361 tccaaaaccc attaagaaag tagaacagcg tgagcctgaa aagtcttttg aagatatttt
    12421 aaaagaaata atacaacaaa atgaaccaaa agttgctccg aaaccaacac caccaccaaa
    12481 acaacagcaa aaaccagtcg taaaatcaaa gccacttaca ccaaatacaa ataaaaggag
    12541 tgaagatcct atattacata atcatgatat tccttcagaa tctattgaat atgatgcaaa
    12601 tatagagaat atcttccata aaagatataa tagtatagaa acctcttcca atagaattga
    12661 agcaaaggaa gatgaaaact ataatgttag aaaaagccaa ggatttaaag gtttgttagt
    12721 agatggaaaa ttactttcag ctaaagaaat gctaatagcc caaactgtat ttgaacgaaa
    12781 attctaaggt taagtctgct tctggcatgt ctctctttgt tgagactaaa tatttcaacc
    12841 tataattat
//
LOCUS       DAOQCW010000071        12827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1811_length_12827_cov_5.781319, whole genome shotgun sequence.
ACCESSION   DAOQCW010000071 DAOQCW010000000
VERSION     DAOQCW010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12827
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1811_length_12827_cov_5.781319"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="PLS16_07525"
     CDS             complement(<1..771)
                     /locus_tag="PLS16_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNJ60968.1"
                     /translation="MQNSFISSYRNFDYLELDSFLTEEQKLIREHVQHFTNHQIIPII
                     DDYAQNAKFPKQLLPELAKLNAFGAMIPSKYGGGGLDAISYGLIMQELERGDSAIRSC
                     ASVQSSLVMLPIYKFGSEEQKQKYLPKLATAEMIGAFGLTEPNHGSDPSSMETRLIKK
                     GDKYILNGAKMWITNAPIADIAVVWAKNENNHVVGCIVEKGMSGFTTPEIKNKWSLRA
                     SITGELIFNDVELSETNILPLAKGLKAPLSCLSSARYGI"
     gene            794..1156
                     /gene="folB"
                     /locus_tag="PLS16_07530"
     CDS             794..1156
                     /gene="folB"
                     /locus_tag="PLS16_07530"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNJ60969.1"
                     /translation="MQVIEKKQSIGLVGMEFYAHHGYYEEEQKIGGQYTVDIYIQYEA
                     EFSEDKLENTINYEIVYQVVKNQMAISSKLIEHIGEQIIIQLKKILPDHHSVKVIIKK
                     HRPPLKGIVNYAIFEIEM"
     gene            1218..1291
                     /locus_tag="PLS16_07535"
     tRNA            1218..1291
                     /locus_tag="PLS16_07535"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1250..1252,aa:Cys,seq:gca)
     gene            complement(1361..3784)
                     /locus_tag="PLS16_07540"
     CDS             complement(1361..3784)
                     /locus_tag="PLS16_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNJ60970.1"
                     /translation="MTKDILDNIDKTLSFKNFKSSILADFRLALMSREVSLIGRREVL
                     AGRAKFGIFGDGKELPQIAMAKVFQNGDFRSGYYRDQTFMLASGLVTVEQLFAQLYAN
                     PSLADDPNSGGRQMNNHFATRSLFPNGDWRNLKDLKNTAADNSPTSSQMPRALGLALA
                     SKKYRQLINVVGNTQFSQNGNEICFATIGDASTSEGLFWESVNAAGVLQVPLIISVWD
                     DGYGISVPKKYQTTKESISDILSGFRYVEGKNGFKIYKAKAWDYVELINIYTKAAQET
                     RIAHIPTLIHIEEATQPQGHSTSGSHERYKSSERLSFEKDYDCITKFKEWIYEFGIAT
                     EDELNEIEQETKQYVGEQRKIAWNKYHQPIINERDEIIQLFSNIQKQQTEFKESIEQI
                     IQLLKNAIDIQRKDIFESIDEVLILLKDLKNEQKIDIIKWRNEQLVVNHERYSSYQFT
                     NSSKSPLKQPVVAKTYDENSKILSAYQLLNACFKANFERDKTILAFGEDVGHIGDVNQ
                     AFAGLQEQFGKERVYDTGIREATIIGEGIGLSMRGLRPIAEIQYLDYLLYGLQPLTDD
                     VACLSYRSKGGQKAPLIVRTRGHRLEGIWHTGSPMQMLLGSLRGMHLCVPRNMTQAAG
                     FYNLLLRSDEPALVIECLNGYRLKEKIPANVGEFTVPLGVPEILNEGSDITLVTYGSC
                     VRIAQKAVLTLEELGISVELIDVQTLLPFDIHGIIGKSVMKTNRVVFLDEDYPGGATA
                     YMMQKVIEQQDIFNYLDVAPQTITAKEHRTSFGTDGDYFCKPNQQDIVRKLYAIMHDV
                     NPTKYNQIF"
     gene            3968..4390
                     /locus_tag="PLS16_07545"
     CDS             3968..4390
                     /locus_tag="PLS16_07545"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNJ60971.1"
                     /translation="MKKIFYLLAITLCTSLAFAQTGSAIKFNETEFDFGNHPQGKPIT
                     HEFFFTNTGSEPIVLESVKASCGCTTPDWSKEPVMPGKTGSVKAQYNMSREGTFRKSI
                     NVTTKGGENVVLYISGNAIPQTNGVEGADQSIIGGGQK"
     gene            4472..5677
                     /locus_tag="PLS16_07550"
     CDS             4472..5677
                     /locus_tag="PLS16_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNJ60972.1"
                     /translation="MPKVSEKGLKMPSSPIRKLAPYADEARKKGVYIYQLNIGQPDIE
                     TPSIFWDAVKQIDRKILEYSPSNGYESLRNRYTRYFQEKCGLETLLPEDVLITTGASE
                     ALFFTLLSILDEGEEVIIPEPLYANYIGFSKSGNIKVVPIATSFENGFNLPSIEEFEK
                     VITPKTKAILICNPNNPTGYTYTFEELERLKNLVKKHDLFIISDEVYREFIYSDQAHI
                     SMFNFQEIEQHVILVDSISKRFSACGARIGMVASKNKLVLETVLKFAQQRLSPPSIEQ
                     LAATALFDVEEDYFKKVNTEYKKRRDTIVAGLNKIEGVKCHLPKGAFYCMVQLPVEDA
                     EHFCKWMLSDFSFQNTTVMMAPGTGFYATPEKGKNEVRIAYVLNSEELEKAAICLGEG
                     LKAYQQIHT"
     gene            complement(5678..6439)
                     /locus_tag="PLS16_07555"
     CDS             complement(5678..6439)
                     /locus_tag="PLS16_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNJ60973.1"
                     /translation="MKFTFLGTGTSQGVPVITCHCKVCQSKDKRDKRTRSSLLIQSDQ
                     TTIVIDTGPDFRCQMLRTKVENLDAIVYTHGHKDHVAGLDDIRPFNYMLDKTIGVYAE
                     PLVQEILKKEFSYIFASNKYPGVPQIEVHTIDTTAFKINDIHFLPIRAMHKDLPVLGF
                     RIADFTYITDANFIADSELEKIKGTEVLVLNALRREKHYSHFSLSEAIELATKIGAKQ
                     TYFTHISHHLGLYQEVNQELPEGIQLAYDGLTIEL"
     gene            complement(6472..6750)
                     /locus_tag="PLS16_07560"
     CDS             complement(6472..6750)
                     /locus_tag="PLS16_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60974.1"
                     /translation="MAFRYNTATLQLIESIFKQNKYVVRYEKGAFQSGYCVLQDKRVV
                     VINKFYDTESRINALVEIFQQLELTNTDFDEKQMKIFSELAQRKLEIE"
     gene            7112..7846
                     /locus_tag="PLS16_07565"
     CDS             7112..7846
                     /locus_tag="PLS16_07565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ60975.1"
                     /translation="MYLYTNWAATIPMANEEEDFAPFVDLLSRVMDVVGTGKIYFVID
                     NASKDKTLELARDLEKKDNRFEVIWAPDNKNVVDAYLKGFEVAYNNGHDIIIEMDAGL
                     SHDPRAIPMYLRVLNEGNECAFGSRFIKDGSMGDSPWRRRFLSKTGTTLANLLLGTKL
                     FDMTSGYQGFHREIIGKLLAYPLKSRAHFYQTEVKYLLRKHRIAEIPIHYQAPSPRVS
                     SKAIKNAYSTLFWYFIQRLKGNTPTI"
     gene            7871..8878
                     /locus_tag="PLS16_07570"
     CDS             7871..8878
                     /locus_tag="PLS16_07570"
                     /inference="COORDINATES: protein motif:HMM:NF015350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STELLO glycosyltransferase family protein"
                     /protein_id="HNJ60976.1"
                     /translation="MKKALIITSIANDQHPVLQLYAKKCAEMEMPFICIGDTKSPDKF
                     NLDGCDFWSVERQLTLDSKFAQICPTRHYARKNIGYILAIQKGYTELVETDDDNLPKE
                     EFWEFKNQRNIVAYDVKDTGWVNIYKYFSNQFIWPRGLPLQELQKQHEALESFTLKTI
                     DCPIQQGLADENPDVDAVYRLTYPLPLNFDFAYNIALGNNAWSPFNSQNTQWYKDAFP
                     LLYLPAYCSFRMTDIWRSFVAQRIAWENDWSILYHKATVWQERNEHNLLKDFEDEIPG
                     YLNNAKIATDLMNLNLKGGKDNLLNDLIACYDMLTKNGYVGKEEMQLVQAWADDLNKF
                     M"
     gene            9318..10466
                     /locus_tag="PLS16_07575"
     CDS             9318..10466
                     /locus_tag="PLS16_07575"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNJ60977.1"
                     /translation="MSNEKLYFKNLDSIRFIAALMVFLQHAVSPSYQYLPIKNTFWEK
                     LLITMSNGGTGVSIFFVLSGFLITYLLISEYELNSKISIKNFYIRRLLRIWPLYFLVV
                     AFSFLIYPFLKSLIGMNNPLGSNFLYHLTFLSNFDVINIEKHCFGSDAMSQNITWSVS
                     IEEQFYLFWPLIFMFLPKRLWVYSISIVILGSILFRIINNNDGVVLYFHTFSVLLDLG
                     IGGLMAFLIKSNKQFRSLFENSSTLTHITLFIFSFCLVFWGDKLFFFNYGAAIGRVFV
                     SFSFALIISAQAITKRKSKLDLGNMSFANKWGKYTYGIYLIHPIAITIIDILVRTMHF
                     PKTNFIALFSIGTIGFVLTLFLSKISFRYYESRFLSLKEKYTTIQTRE"
     gene            10776..11411
                     /locus_tag="PLS16_07580"
     CDS             10776..11411
                     /locus_tag="PLS16_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HNJ60978.1"
                     /translation="MEVRDLFGNIIPSLTIDKTKSGLTKIENGEYLFYPDFFSKAESD
                     KYLQTMKENIEWKQESMNMYGKQVLFPRLTAWYGDNNKPYSFSGITLNPNFWTDELSE
                     IKNKIEPIAQTKFNSVLLNRYRSGNDSISWHTDAEKELGINPIIASVNFGATRKFQLR
                     HIKTKEKIEIELTHGSLLIMQGELQHFWQHQVPKTSKFVGERINLTFRTIL"
     gene            11413..12432
                     /locus_tag="PLS16_07585"
     CDS             11413..12432
                     /locus_tag="PLS16_07585"
                     /inference="COORDINATES: protein motif:HMM:NF020476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HNJ60979.1"
                     /translation="MTWDKLEILTDEGEKVTAQAPVIVSASRSTDIPTFYADWFVERW
                     KKGYIKWINPFNGVPLYVSFKNAKVVVFWTKNPKPMFKHLDFLDVNVKNYYFQFSLND
                     YDKEGYEGKVPRLENRINTFKELSERIGKERVVWRFDPLLLTTEIDIKELLRRLENIG
                     NQIYFFTNKLVFSFADISIYKKVVNNMNKANIDYIEFSPDTMIEFAEGLNKLNKKWNL
                     ELATCAEKFDLDKYGIIHNKCVDDDLMIKLFSHDIELMKFLGIEFTEPTLFEDGESFI
                     KTRKLKDKGQREACGCIMSKDIGEYNTCPHECVYCYANTSFETAKNNYKSHTANPFAE
                     TIIGK"
     gene            12435..>12827
                     /locus_tag="PLS16_07590"
     CDS             12435..>12827
                     /locus_tag="PLS16_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60980.1"
                     /translation="MPYYFNLPLITNLTRDQRRAVDENNPLALSGGPGTGKSVVSLWR
                     HITNHSTGLRNSLLLTYTKTLEHYLKASANTQNVNAGNNIARTNCWLCDCPDNNVERK
                     NFEEIIIDEGQDIIKDTFQRFFNYTNRIS"
BASE COUNT         4423 a         2046 c         2008 g         4350 t
ORIGIN      
        1 aatgccatat cttgcagagg ataaacaaga caatggtgct tttaatcctt ttgccaatgg
       61 caaaatattt gtttcactca attcaacatc attaaaaatt agttcgcctg tgatagatgc
      121 ccgcaaactc catttatttt ttatttcagg tgttgtaaat cctgacattc ctttttcaac
      181 aatacaaccg acaacgtgat tattttcatt ttttgcccaa acaactgcaa tatcggcaat
      241 cggtgcatta gtaatccaca tttttgcccc atttaaaata tacttatctc cttttttaat
      301 tagtctagtt tccatactag atgggtcgct tccatgattg ggttctgtca atccaaaggc
      361 tcctatcatc tcggcagtgg ctaatttagg taagtatttt tgtttctgct cctctgaacc
      421 aaatttataa ataggtaaca tcactaaaga actctgaaca ctagcacagc ttcttattgc
      481 cgaatcccct ctttcgagtt cttgcataat taatccataa gaaatcgcat ctagacctcc
      541 gccaccatat tttgaaggaa tcattgctcc aaaagcatta agctttgcta attctggtaa
      601 taattgtttt ggaaattttg cattttgagc ataatcatca ataataggta tgatttgatg
      661 attagtaaaa tgctgcacat gctctctaat caatttttgt tcttctgtca aaaaactatc
      721 taattctaaa taatcaaaat ttctatatga agatataaac gaattttgca tgatataatg
      781 tatttttgtt gtgatgcaag ttatagaaaa aaaacaaagt attggtttag tcggtatgga
      841 attttatgcc catcatgggt attatgagga agagcaaaaa atcggtggac aatatactgt
      901 tgatatttat attcaatatg aggctgaatt ttcggaagac aaacttgaaa atacgataaa
      961 ttatgaaatt gtctatcaag tggttaaaaa tcaaatggcc atatcttcca aactaataga
     1021 acatattgga gaacaaatta ttatacaact aaaaaaaata cttccagacc atcatagcgt
     1081 caaagttatt ataaaaaaac atcgtcctcc attgaaaggt atcgtaaatt atgcaatatt
     1141 tgaaatagaa atgtgataaa agtttgcata taaagaaaaa ggttttatat ttgcactccg
     1201 ttagtgtaaa ctaatatggt cggttggccg agaggctagg cagagctctg caaaagctcg
     1261 tacggcggtt cgaatccgcc accgacctcc aaaataaaac gcaaattatt gtatatcaat
     1321 agtttgcgtt tttaattttc atcaaaaagt agctaataaa ttagaaaatt tggttgtatt
     1381 tagtaggatt tacgtcatgc attattgcat ataatttccg cactatatct tgttggttgg
     1441 gtttgcaaaa ataatcgcca tctgtaccaa atgaagtacg atgttctttg gctgtaattg
     1501 tttggggagc aacatctaaa taattaaaaa tatcctgttg ttctattact ttttgcatca
     1561 tataagcagt cgctccacct ggataatctt catctaaaaa aacgactcta ttggttttca
     1621 ttacagattt tccaattatt ccatgtatat caaacggcaa taaggtttgc acatctatta
     1681 attctactga tatgcctaat tcttctaagg tcagtactgc tttttgtgca atacgcacac
     1741 atgagccata agttactaaa gtaatatcag aaccttcatt tagaatttct ggtacaccta
     1801 atggcactgt aaattcacca acatttgctg gaatcttctc ttttaaacga taaccattca
     1861 aacactcaat cactaaagct ggttcatcgc tacgcaacaa taaattataa aaacctgcag
     1921 cttgtgtcat atttctagga acacaaagat gcataccacg caaactaccc aaaagcattt
     1981 gcattggcga accagtatgc caaatacctt ccaatcgatg tcctctagtt cgaacgatta
     2041 gtggagcttt ttgtccacct tttgaacggt atgataaaca agcaacatca tcagtgagcg
     2101 gttgtaagcc ataaagcaaa taatccaaat attgaatttc tgcaattgga cgtagtcctc
     2161 gcatagacaa gcctattccc tctcctatta ttgttgcttc tcttattcct gtatcataaa
     2221 ctctttcttt cccaaactgt tcctgcaaac cagcaaaagc ttgattaaca tctccaatat
     2281 gtcctacatc ttcgccaaaa gcaagtattg ttttatcacg ttcaaaattc gctttaaaac
     2341 atgcatttaa taattgataa gcacttaaaa tttttgaatt ttcatcatag gtttttgcaa
     2401 ctacaggttg tttcaatgga gattttgatg aatttgtaaa ttgataagaa ctatatcgct
     2461 catgatttac caccaactgc tcatttctcc attttatgat gtcaattttt tgttcatttt
     2521 ttaaatcttt caataaaatc agtacttcat ctatcgattc aaaaatatct tttctttgaa
     2581 tatctattgc atttttaagc aattgaataa tctgttcaat tgactcttta aattctgttt
     2641 gttgtttttg aatatttgaa aacaactgaa taatttcatc acgttcatta ataattggtt
     2701 ggtggtattt attccaagca atttttcttt gctcaccaac atattgttta gtttcttgtt
     2761 ctatttcatt taattcatct tctgttgcaa ttccaaattc ataaatccat tctttaaatt
     2821 ttgtaatgca atcatagtct ttctcgaatg ataatctttc tgatgattta tacctttcat
     2881 gcgaaccact tgtggagtgt ccttgtggtt gggttgcttc ttcaatatga ataagtgttg
     2941 gaatatgagc tattcttgtt tcctgtgctg cttttgtata tatattgata agttcaacat
     3001 aatcccacgc ttttgcttta tagattttaa aaccattttt accttctaca tatcgaaatc
     3061 cacttaatat atcgctaata ctttctttag tcgtttgata ttttttggga acagaaatac
     3121 cgtatccatc gtcccaaaca gaaataatta aaggcacttg caaaacacct gcagcattta
     3181 cagattccca aaataaacct tcagatgttg atgcatcgcc aattgtagca aaacatattt
     3241 catttccatt ttgactaaat tgtgtattgc ctacaacatt tattaattgt ctatattttt
     3301 ttgatgctaa tgccaatcct aatgctcgtg gcatttgaga gcttgtagga gaattatcag
     3361 ctgccgtatt ttttaaatct ttcaaatttc gccagtcacc atttgggaat aaacttcttg
     3421 ttgcaaagtg attattcatt tggcgaccac cagaatttgg gtcatcagcg agcgatggat
     3481 tcgcatacaa ttgtgcaaat aattgttcga ctgttactaa accactagcc agcataaaag
     3541 tttggtcacg gtaatagcct gagcgaaaat ctccattttg aaatactttt gccatagcaa
     3601 tttgaggtaa ttccttacca tcaccaaaaa ttccaaactt ggctctacct gctaaaacct
     3661 ctcttcttcc aatcaaactc acttctctac tcatcaatgc caacctaaaa tcagcaagta
     3721 tgctactttt aaaattttta aaagaaagtg ttttatcaat attatccaaa atgtcctttg
     3781 tcatttgcaa cataaaaaat atcctatatc tatttacaca aatttaatat aaaagtttat
     3841 atatgcacca aaaatctcaa aggactagac caatcaatat ctacattcgt ttattattta
     3901 taaacaaaga atataatctt aaaatttact ggcataattt ttgttattaa tttagaaaag
     3961 aatttttatg aaaaaaatat tttatttatt agctatcacg ttgtgtacat cattggcttt
     4021 tgctcaaaca ggttctgcta ttaagtttaa cgaaacagaa tttgattttg gcaatcatcc
     4081 gcaaggaaaa ccaattacac acgaattctt ttttaccaat acgggttctg agccaatagt
     4141 tttagaaagc gtaaaggctt cttgtggttg cactactcca gattggagta aagagcctgt
     4201 tatgcctggc aaaactggtt ctgtaaaggc acaatacaat atgtctagag aaggaacttt
     4261 tagaaagtct ataaatgtaa ccacaaaagg tggtgagaac gttgtactat atatttctgg
     4321 taatgctatt cctcaaacaa atggcgttga aggtgcggac caaagtatta ttggtggtgg
     4381 gcaaaagtaa tagcacaacc tatattaaat tttagtttaa attcaaactc catgcttttt
     4441 agtatggagt ttttttaatt ttgtacttat tatgccaaaa gtatcagaaa aagggctaaa
     4501 aatgccttca tctcctatca gaaaactcgc accttatgct gatgaagcac gaaaaaaagg
     4561 tgtgtatatc taccaactta atattggaca acccgatatt gaaacgccaa gtattttttg
     4621 ggatgccgta aaacagatag atagaaaaat attagaatat agtccgtcta atggttatga
     4681 aagccttaga aatcgttata cacgctattt tcaagaaaaa tgtggtctag aaaccttatt
     4741 acctgaagac gttttgatta ctactggtgc atctgaagca ttatttttta cattattatc
     4801 gattttagat gaaggtgaag aagtaatcat tccagaacct ttatatgcca actacatcgg
     4861 attttctaaa agtggaaata taaaagttgt tccaattgca acctcttttg agaatggatt
     4921 taacttacca tctatcgaag aatttgagaa agtaattact ccaaaaacta aagctatact
     4981 tatttgtaat ccaaacaatc ctactggata tacttatact ttcgaagaat tagaaagact
     5041 aaaaaactta gtgaaaaaac acgatttatt tatcattagc gatgaggttt atcgtgagtt
     5101 tatttattca gaccaagctc atatcagtat gttcaacttc caagagattg aacagcatgt
     5161 aatcttagta gattctattt ctaaaagatt tagtgcttgt ggtgcaagga ttggaatggt
     5221 ggcttcaaaa aataaattgg tactcgaaac cgttttaaaa tttgctcaac aaagattaag
     5281 tccccctagt attgaacaat tggcagcaac tgctttattt gatgtagaag aagattattt
     5341 taaaaaagta aatacagaat acaaaaaaag acgagatact attgttgcag ggttgaataa
     5401 aatcgaaggt gtaaaatgcc atttacctaa aggtgctttt tactgcatgg tacaattgcc
     5461 cgtagaagat gcagaacatt tttgcaaatg gatgttgagt gatttctcat ttcaaaatac
     5521 aactgtaatg atggctccag gaactggttt ttatgctact cctgaaaaag gtaaaaatga
     5581 agtacggatt gcttatgttt taaattcaga agaattggaa aaagctgcta tttgcttagg
     5641 tgaaggttta aaagcatacc aacaaataca cacttaacta caattcaatc gttagtccat
     5701 cataagctaa ttgaataccc tctggtaatt cttgattaac ttcttgatac aaacctaaat
     5761 ggtgtgaaat atgtgtaaaa taagtttgct tcgcaccaat ttttgtagct aactcaattg
     5821 cttctgataa agaaaaatga gaataatgtt tttctctacg aagtgcattt aatactaaaa
     5881 cctctgtgcc tttaatcttt tctaactctg agtctgcaat aaaattagcg tctgtaatgt
     5941 atgtaaaatc tgctattcta aaccccaaaa ctggcaaatc tttgtgcata gctcgtatag
     6001 gtaaaaaatg aatatcgttt atcttaaaag cagtcgtatc aatagtatgt acttcaattt
     6061 gaggaacacc tgggtattta ttgctagcaa aaatataaga aaactctttt tttaaaatct
     6121 cttgtactaa tggttctgca taaacgccaa ttgttttatc taacatataa ttaaaaggtc
     6181 ggatatcatc taaaccagca acatggtctt tatgtccatg tgtataaact atggcatcta
     6241 aattttcaac ttttgtacgc aacatttgac acctaaaatc tggaccagta tcaataacaa
     6301 tagtagtttg gtcagactgt attaaaagtg aactccttgt tcttttatct cttttatctt
     6361 tagattgaca gactttacaa tgacatgtaa ttactggcac accttgtgat gttccagttc
     6421 ctaaaaaagt aaatttcatt ttcaaaaatt cgtattatac aatgatatgt actattcaat
     6481 ttccaatttt ctttgagcca attcactaaa aattttcatt tgtttttcat caaaatctgt
     6541 atttgttagt tctaattgct gaaaaatttc taccaatgca tttattcttg attcggtatc
     6601 atagaattta ttaattacta ccactcgttt atcttgcaac acacaataac ctgattgaaa
     6661 agcacctttt tcatatctta ccacatactt attctgtttg aagatagatt caatcaattg
     6721 aagcgtagca gtattgtatc tgaaagccat actacaaaag tacaaagtat tttacaatga
     6781 ataatcaaat ttttgtccac aataaaacaa gtgtttttcc ataaaaaagt ctaagaccgt
     6841 tgcaaggtaa tcattattca taaatctcaa gtcaaattcc ttcaatcttc ttcgttactc
     6901 atctattttt aatcctcatt atcaatatga taactgcggt gtaaaaataa atgagagcct
     6961 tgaatatttt tgtaatttga cttttgcaac ggtcttagtc tatatataag aaagcaaaat
     7021 tcttttaaca gatatttttg tttgtatatt atataaatct ttttaaatga gaaaaaagcc
     7081 gtactttagc aactcaaaat aagtaaaaat catgtattta tatacgaatt gggcagcgac
     7141 tattccgatg gcaaatgaag aggaagattt tgcacctttt gtagatttat taagtcgagt
     7201 aatggatgtt gtgggtactg gaaaaatata ttttgtaata gacaatgcct ccaaagataa
     7261 gactttagaa ttagcaagag atttagagaa aaaagacaat cgttttgaag tcatttgggc
     7321 tccagataat aaaaatgttg ttgatgctta cttaaaaggt tttgaagttg cctacaataa
     7381 tggacatgat atcattattg agatggatgc tggtctaagc cacgatccaa gagcaattcc
     7441 tatgtattta cgagttttaa acgagggtaa tgaatgtgct tttggaagtc gttttataaa
     7501 agatggttct atgggagatt ctccgtggcg tagaagattt ttatcaaaga caggtactac
     7561 tttagcaaat ttactattag gtacaaaatt attcgatatg acttctggct atcaaggatt
     7621 tcacagggaa atcattggta aattattagc ttatccatta aaatccagag ctcattttta
     7681 ccaaactgaa gtaaaatatc tgttgagaaa acatcgtatt gctgaaattc caatccacta
     7741 ccaagcacct tcacctagag tttcatctaa agccataaaa aatgcttaca gtacattatt
     7801 ttggtatttt attcaaagat taaaaggaaa tacgcctaca atttaaatcc actagtttaa
     7861 ttaaacaaca atgaaaaaag cacttattat tacgtccatt gccaatgacc aacatccagt
     7921 attacaatta tatgcaaaga aatgtgctga aatggaaatg ccttttatat gtattggcga
     7981 cacaaaatct ccagataaat ttaacttaga tggttgtgat ttttggagtg ttgaacgtca
     8041 gcttacatta gattcaaaat ttgcacaaat ttgtccaaca agacattatg ccagaaaaaa
     8101 tattggttac attttagcaa ttcaaaaagg ttatactgaa ttggttgaaa ccgatgatga
     8161 taacttgcca aaagaggaat tttgggaatt taaaaaccaa agaaatattg tggcttatga
     8221 tgtaaaagat acaggttggg taaatatcta caaatatttc agtaatcaat ttatttggcc
     8281 tagaggatta cctttgcaag aattacaaaa acaacacgaa gccctagaaa gctttacttt
     8341 aaaaacgatt gattgtccta tccaacaagg cttagcggat gaaaatccag atgtcgatgc
     8401 ggtatatcgt ctaacttatc cattgccttt aaactttgat tttgcataca atattgcttt
     8461 aggaaataat gcttggagtc catttaatag tcaaaataca caatggtaca aagatgcatt
     8521 tcctttacta tacttaccag catattgtag ttttagaatg actgatattt ggagaagttt
     8581 tgtagctcaa agaattgctt gggaaaatga ttggagtatt ttgtaccaca aagccactgt
     8641 atggcaagaa agaaatgagc ataatctttt aaaagatttt gaagatgaaa ttccaggtta
     8701 tttgaacaat gctaaaatag caacagattt aatgaatttg aatttaaaag gtgggaaaga
     8761 taatttatta aatgatttaa ttgcttgtta tgatatgctt acaaaaaatg gctatgtagg
     8821 aaaagaggaa atgcaattgg tacaagcatg ggcagatgat ttgaataagt ttatgtaacc
     8881 taccaacata gagaaaataa aagaggctgt ccgaaatttt ggacagcttt tttttaagat
     8941 tcagattgaa ttagatgtgc aagcaatttg tatttctgat aatttcttct ttctaaattg
     9001 gtttacatta acaactaaat aaatgtgtaa aaatggaatc caataaagcc ttttaggata
     9061 atacttttag acggcggctt tagaattcaa atcccatcat atattataca tattgttata
     9121 aaaaatacga aatacactat tgcgtactac attacctaaa tataaataac acgcaataag
     9181 acatctgtat atatttaatt aacttacaat ttgttagcaa ttttatgcta ttttttttta
     9241 taatttaaaa gtcatttatt atatttgcgt atataaagga gttagcggta agcaaaaaag
     9301 acacgaccaa gacaattatg agtaacgaaa aattatattt taagaattta gattctatta
     9361 gatttatagc tgcattaatg gttttccttc aacatgctgt tagcccatcg tatcaatatc
     9421 tgccaattaa aaataccttt tgggagaaac tgctcatcac aatgagtaat ggagggacag
     9481 gggtttcaat attttttgta ttgtctggtt ttctaatcac ttatcttctt ataagcgaat
     9541 atgagttaaa ttcaaaaata tctattaaaa atttttacat caggcgctta ctaagaattt
     9601 ggcctctata ttttttagtc gttgcttttt cttttttgat ttatcctttt ctaaaatcat
     9661 taatcggaat gaacaaccct ctgggttcaa attttcttta tcacttaact tttttgagca
     9721 attttgatgt gataaatatt gaaaagcatt gctttggaag tgatgcaatg tcgcaaaaca
     9781 taacttggtc ggtttctatt gaagaacaat tttatttgtt ttggccatta atatttatgt
     9841 ttttaccaaa acgactttgg gtttactcaa tatcaattgt catactcggt tcaatacttt
     9901 ttcgcattat aaacaacaat gatggtgttg ttttatactt tcatactttc tcagtgttgc
     9961 ttgaccttgg tattggtgga cttatggctt ttttaataaa atcaaataaa caattcagga
    10021 gtttatttga aaatagttca acattgactc atattacttt atttattttt tcattttgtt
    10081 tagttttctg gggagataaa ctgttcttct ttaattatgg agctgctatt ggtcgtgttt
    10141 ttgtatcatt ttcctttgca ttaataattt ctgcccaagc cattacaaaa agaaaatcaa
    10201 aacttgacct tgggaatatg tcatttgcaa acaaatgggg aaaatatact tatggtattt
    10261 atttaattca tccaatagct attactatta ttgacatctt agttcggaca atgcatttcc
    10321 caaaaactaa ttttatcgct ttattttcta ttggcactat tggttttgtc cttactttat
    10381 ttttaagtaa aattagtttt agatattatg aatcaagatt tctttcatta aaagaaaaat
    10441 acacaacaat acaaacacgt gaatagattg ccaaccgcta acagcacatt tgcaataggc
    10501 ggggtttcgt gctccgcaga cagttaagcg gtagccgaaa aatttgtgct ccgcatcaac
    10561 atttgtgctg aaaagcccgc ccatcgcaaa tctgcaaaac gttatgcctc accctataaa
    10621 agacagtgca aacattaaac gacagtaaaa atgaacagac aagccaatgc ttcggcaaaa
    10681 tcaaaagagc tgcaacctgc cgcacaaagc ctacgctttg cagcacattt gcttttgccc
    10741 gaacacacat acttcacagt aaaaaaatca agaaaatgga agtaagagac ctttttggca
    10801 atattatccc ttctttgacc attgataaaa caaaatcagg tttaacaaaa attgaaaatg
    10861 gtgaatactt attttatcct gatttctttt caaaagcgga aagtgataag tatttacaaa
    10921 caatgaagga aaatattgaa tggaaacaag aatcgatgaa tatgtatgga aaacaagttt
    10981 tgttccctcg tttgactgct tggtatggtg acaataacaa accttattca ttttctggaa
    11041 ttacccttaa tcccaatttt tggactgatg aattatctga aataaaaaat aaaattgaac
    11101 ctattgcaca gactaaattc aatagtgttt tattaaatcg ttatagaagt ggaaatgatt
    11161 ctatttcttg gcatactgat gctgaaaaag aattaggcat taacccaatt attgcttcgg
    11221 taaactttgg agctacaaga aagtttcagc taagacatat taaaacaaaa gagaaaattg
    11281 aaattgaatt aactcacgga agccttttaa taatgcaggg agaattacaa catttttggc
    11341 aacatcaagt tccaaaaaca agtaaatttg ttggtgagag aattaatcta acttttagaa
    11401 caatattgta aaatgacttg ggataagctc gaaatattaa ctgatgaagg agagaaagta
    11461 acagctcaag ctcctgttat tgtttctgcg agcagatcaa ctgatatacc tacattttat
    11521 gcagattggt ttgttgagcg atggaaaaaa gggtatatca aatggataaa tccttttaat
    11581 ggtgtaccgc tatatgtttc ttttaaaaat gcaaaagttg tagttttttg gacgaagaat
    11641 ccaaaaccga tgtttaagca tttagacttt cttgatgtaa atgtaaagaa ctattatttt
    11701 caatttagct tgaatgatta tgacaaggaa ggatatgaag gcaaagttcc aagattggaa
    11761 aatagaataa acacttttaa agagttatct gaaagaatag gaaaagaaag ggtagtttgg
    11821 agatttgacc cattattact tactactgaa attgatataa aagaactact aagaagactt
    11881 gaaaatatag gcaatcaaat ttatttcttt accaataaac tagttttcag ttttgctgat
    11941 atatcgattt ataaaaaagt agtaaacaat atgaataaag caaatattga ctacattgaa
    12001 ttttctcctg acactatgat tgaatttgct gaaggattga ataaactcaa taaaaaatgg
    12061 aatcttgaat tggctacttg tgctgaaaaa tttgatttag ataaatatgg cattattcac
    12121 aataaatgtg tggatgatga tttaatgata aagttattta gccacgacat agaattgatg
    12181 aagtttttag gtatagaatt tacagaacct acactttttg aagatggaga aagttttatt
    12241 aaaacaagaa aactaaaaga caaaggacag cgtgaagctt gtggttgtat tatgagcaaa
    12301 gatattggtg aatacaacac ttgcccacac gaatgtgtat attgctatgc aaatacatct
    12361 tttgaaactg caaaaaacaa ttataaatca catactgcaa atccgtttgc agaaacaata
    12421 atcggaaaat aattatgccg tactatttta atttaccact tattacaaat ttaacacgag
    12481 accaacgaag agctgttgat gaaaataatc cacttgcctt gtctggtgga ccgggaacag
    12541 gaaaaagtgt agttagttta tggcgtcaca tcactaatca ttcaacagga ttacgaaata
    12601 gcttattact aacatatact aagactttag aacattatct gaaagcttct gctaatacac
    12661 aaaatgttaa tgcaggaaat aatattgcta ggacaaattg ctggctatgt gattgtccag
    12721 ataataacgt agaaagaaaa aattttgaag agattatcat tgatgaagga caagatataa
    12781 taaaagatac atttcagcga ttttttaatt atactaatag aatctct
//
LOCUS       DAOQCW010000072        12708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1834_length_12708_cov_4.724808, whole genome shotgun sequence.
ACCESSION   DAOQCW010000072 DAOQCW010000000
VERSION     DAOQCW010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12708
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1834_length_12708_cov_4.724808"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="PLS16_07595"
     CDS             complement(<1..698)
                     /locus_tag="PLS16_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNJ60981.1"
                     /translation="MTTFKNKTVFISGGTRGIGHAIGMRLAQEGANIVIAAKSTEPNP
                     KIPGTIFTAAADMEKAGGKALAVKCDIRDEAEVINAVNKAVETFGGIDILINNASAIN
                     LSPTLALEMKRYDLMQDINTRGTFLCSQKCIPYLLKAENPHILNLSPPISSINEHWFK
                     NHVGYSIAKFGMSLCVLGMSGEYRGKIGINALWPRTTIATAAVQNLLGGNSIMNASRK
                     PEILADAAYYILKR"
     gene            804..2195
                     /gene="lpdA"
                     /locus_tag="PLS16_07600"
     CDS             804..2195
                     /gene="lpdA"
                     /locus_tag="PLS16_07600"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNJ60982.1"
                     /translation="MKDFDVIILGAGPGGYVAAIRLAQLSYKVAIVEKENLGGICLNW
                     GCIPTKALLKSAHLFKEIKSAHQYGISIDEVKADFTKIIERSRNIANEMQNGIQFLMK
                     KNKIEVIKGYGKILPGRRVEVTNNEAQILIYKSDAIILATGSSPKNIASLPIDHQYII
                     DYKDAMSIKKQPKELIIIGAGAIGVEFAYFYQSIGTQVTIIEFSEHILPNEDVDVSLA
                     LKKSLEKQGIKIYTSSKVLNSKIENNEVVLLVNTPEQEIILKSECVLSAVGIAANLDN
                     IGLELCNIETERDKIKTNIYCQTNVDGVYAIGDIIGGQALAHTASAEAIIAAEHFAGL
                     STLPIDYNNVPSCVYCSPEIASVGYTEVQLIKNKIDYLVGKFPFSASGKAKAIGETKG
                     FVKVLVDKKYDEILGVHIIGTNATEIIEEIVLARSLEATGKSILHSIHPHPTINEAIK
                     EAFENAYKQSIHL"
     gene            complement(2203..2727)
                     /locus_tag="PLS16_07605"
     CDS             complement(2203..2727)
                     /locus_tag="PLS16_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HNJ60983.1"
                     /translation="MRKLKLDELNRISRDEFKESIKNPIVIVLDNVRSAMNVGSVFRT
                     SDAFLVEEIILVGITAQPPNREIQKSALGATETVDWRYFSTTEDCIQYLKEKNYTILS
                     IEQSDHSTMLNEFKIEDNKQYALVLGNEVDGVSYEFINESVACIEVPQFGTKHSLNIS
                     VCAGIVIWHFVSAY"
     gene            2870..3586
                     /gene="bshB1"
                     /locus_tag="PLS16_07610"
     CDS             2870..3586
                     /gene="bshB1"
                     /locus_tag="PLS16_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HNJ60984.1"
                     /translation="MKLDILVFAAHPDDAELACGGTIAKHVKNGYKVGIIDLTNGELS
                     SRGNVDLRRKEAQKAAEILGLSVRENLGFRDGFFEIDEYHLLEIVKKIRQYQPAIVLC
                     NAIDDRHPDHKRGGDLVERAFFLSGLVKIETEINGVAQSIWRPKKLIHYIQEKYIKPS
                     FVVDISDFLEVKFKSIEAYTSQFYQKDSDEPYTKISGEGYFEFLKSRAKELGSQSGYD
                     YAEGFVSKTLIGIDNIIDIE"
     gene            complement(3655..4629)
                     /gene="meaB"
                     /locus_tag="PLS16_07615"
     CDS             complement(3655..4629)
                     /gene="meaB"
                     /locus_tag="PLS16_07615"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNJ60985.1"
                     /translation="MIEPLSYYIDGINNKDRIVLSRAITLIESTVPAHRVVANQLIEY
                     CLKQEKQSKRIGITGSPGVGKSTFINALGAEIIANSQSLAILAIDPSSTITKGSILGD
                     KTRMYEIAYHKDVFIRPTASSNYLGGIAQNTYQTILLCEAFGFDYILIETVGVGQSEI
                     EIKHLTDLFMLLVLPNSGDELQGIKRGVMELADIIIINKADKANEQQAKISVAQLKSV
                     LPLLNYQNKEWNNIVLQTSSIEKIGIDNTLKYIESYFSKVNIESIRKQQLLSWKNNLV
                     IQLIQEKIMSNKNIKTIIDEFLANDAYSYKQIQKINTQLESLINKLDS"
     gene            complement(4666..5868)
                     /locus_tag="PLS16_07620"
     CDS             complement(4666..5868)
                     /locus_tag="PLS16_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNJ60986.1"
                     /translation="MTTSIIDRIKQSANEFSTQLIEIRRHLHTHPELSFEEKETAKFI
                     ATQLDKLGITYQKDIGGHGIVGLIKGNNPDQQTIALRADIDALPIHEKNKVSYCSVNE
                     GKMHACGHDVHTTCLIGALKVLNEIKDNFEGQIKFIFQPAEEKLPGGASILIQEGVLE
                     NPKVEKIFGEHVLPQLETGKVAFRSGISMASCDEIFITIKGNGGHGAVPNLAVDTVLI
                     ASHIVIALQQIVSRNANPIMPSVLTIGKFIANGATNVIPEEVKMEGTFRTFDEKWRAD
                     AKIKIVQIATSIAESMGAEIEIDIAHGYPFLKNDEEITQKAIANAKLYLGEQNVEELP
                     IRMTAEDFSYYTQVVPACFYRLGTGNIEKGITSSIHTPTFDVDENCLEIGAGLMAWLA
                     FRELSNGK"
     gene            complement(5966..6781)
                     /locus_tag="PLS16_07625"
     CDS             complement(5966..6781)
                     /locus_tag="PLS16_07625"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNJ60987.1"
                     /translation="MKKTFLLTLMIGVMGFTSNIFAETIEDIVAKADAAMKAVKTCSY
                     DFYSKERFKGGKVISSYIQFKIQESPVKKTYANSIEPQKAQLVYIPSVQSKVKVKKGF
                     TLNLELTNGLLMKEQHQTIDRAGFGRIRKVLMTSIDARKGQDLDKFAKITGSVNYDGK
                     DCYRIEINDPDYKIILHTVTPTQTSVWKLGDQLAIPEYKIKELNGLSKDELTPGQKIK
                     IPSSYAKKTIIYLDKKTYLPLYQRMEDAEGMYETYEMKNLKIGVSLTDADFQF"
     gene            complement(6851..8197)
                     /gene="argH"
                     /locus_tag="PLS16_07630"
     CDS             complement(6851..8197)
                     /gene="argH"
                     /locus_tag="PLS16_07630"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNJ60988.1"
                     /translation="MAKIWQKKEIDNSELSKLVETFTVGKDRELDLILAKYDVLGSLA
                     HTQMLAEVGLLPKEELVPIHKELKNIYNTIEAGEFTLDESSEDIHSEVEYRLTQKLGD
                     LGKKIHSGRSRNDQVLLDIKLYSRAKIKETVEQTQILFDTLIGLAEKHKDVLLPGYTH
                     LQIAMPSSFGLWFSAYAESLSDDINSLYGAYALVNKNPLGSGAGYGSSFPLNRIRTTE
                     LLGFESLNYNVVYAQMNRGKMEKFVSSALANIASSLAKLAMDATLYINQNFGFISFPD
                     VLTTGSSIMPHKKNPDVFELIRAKCNKLINLPTQISMITGNLPSGYHRDLQIIKEDYL
                     YSFDTLIDCIKMTNTMLEYIIIKEQILEDDRYKYLFTVDSINELVNQGQSFRDAYVEV
                     GQKVENGSYEKPNELKHTLEGSIGNLCLDEIVKKMIKNTEQFHFTKFLEKENCLVQ"
     gene            8379..11171
                     /gene="polA"
                     /locus_tag="PLS16_07635"
     CDS             8379..11171
                     /gene="polA"
                     /locus_tag="PLS16_07635"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNJ60989.1"
                     /translation="MEKLYLFDSFAMIFRAYFALQKNPLINSKGQNVSAITGFLNTLY
                     EIITKYKPTHIACAFDSTEPTIRHEAFEAYKANRSETPEDIIFSIPYIKAIIKGFNIP
                     ILEVPGYEADDIIGAIAMQAEQHNIETYIVTMDKDLGQLVSDKTFIHRPPYMGKPAQI
                     YGVQEICERWEIDNPKQLIDILGLMGDAVDNIPGVAGIGEKTAQKLIKDFGSIEGIYE
                     NIEQIKGKLKDKLVEQKEQAYMSKSLATVELNVPVEFHIETFKIQEKNTVELAEIFAE
                     LEFRTQAKRILGDSYNLVEEKNKASINNNQQFDLFSSISETTSATENISNNKNLYNTE
                     HQYSLIDSENKLAELIQKIAKEKSICFDTETTSLNTFESELVGISISLKENEGFYIPC
                     PTNKQQTQAIIEQLRPILENENITKIGQNIKYDLLVLKNYNIELKGILWDTMLAHYLI
                     EPDARHGMDILAENYLNYQTISIEELIGKKGKNQGNMRDVPIEKIKDYAIEDADITLQ
                     LQQIFKKEIDQTHLENLFYNVETKLITVLADMEREGINVDVPFLQQYSSEIQEELIQL
                     QDTIYKKSGTTFNIDSPKQLGEILFEHLKIPYQGKKTKTGQYSTDEATLQKFFDKHPI
                     IQSILDYREFTKLKSTYVDAIPNLINPLTNRIHTTYNQTSVATGRLSSINPNLQNIPI
                     RTERGKQIRKAFIPRDENYTLLSADYSQIELRIVASISGDENMMEAFRQQQDIHSATA
                     ARVFNVSLEEVSSEMRRKAKMVNFGIIYGISAFGLAQRLGTGRNEAAELIEEYFKQYP
                     KIKNYMDNTILFAQQNGYVETLLKRRNYIRDINSENYTIRGFAERIAINAPIQGSAAD
                     MIKVAMIDIHQQLKSLKCHSKLLLQVHDELILDVHKSEKEIIEKIVISSMKNALPLNI
                     PVEVSCGWGNNWLEAH"
     gene            11371..>12708
                     /locus_tag="PLS16_07640"
     CDS             11371..>12708
                     /locus_tag="PLS16_07640"
                     /inference="COORDINATES: protein motif:HMM:NF024574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ60990.1"
                     /translation="MKSRLFLFVFIILSFFGFSANWDATYISKLLAKKEYDKIINYYK
                     KEYTQKPEYAFKIADVYAKKKDYATAISWYQKEPHLLKETKVNLINLAKTYKEMGDYQ
                     KALDTYITYAAESGDATKVLKDAQLCEKLIRASSFTDNYLIEPYTFNTAVNEINLSIL
                     RANLIYLQRDFENEKRKNDSPNSVTLQAQRMFDKWNEPIKIIQQEETNKEYIKFSFTK
                     DGNKVVFSAKPATLLPNKKESNKKPKLYDQIFVADFLGGQLFNIKSFPYNSEEYSCAN
                     PSFNNDGTKIIFSSDMSNKTDGFDIYESELIEGKWTKPKNLGNLLNSPKNDNHPFYLK
                     EKNNKEYLYFSSDREDGFGGYDIYRAEYSDGLWQDVQLLPAPINTAGNEDSYTYDLET
                     QTAYVSSDRSDSKGGFDIYRIKPLNLLLFITVKDNDTKDPLGFAFLELFEKNNQ"
BASE COUNT         4515 a         1940 c         1866 g         4387 t
ORIGIN      
        1 tctcttttta aaatataata ggctgcatct gctaatattt ctggctttct agaagcattc
       61 ataatagaat ttcctcccaa taaattttgt actgctgccg tagcaatggt tgttcttggc
      121 cataatgcat tgataccaat cttaccacga tattcgccac tcattcctaa cacacataaa
      181 ctcattccaa attttgcaat cgagtagcct acatgatttt taaaccaatg ttcattaata
      241 gaactaattg gtggcgataa atttaagatg tgtgggtttt ctgcttttaa taaataagga
      301 atacattttt gtgagcaaag gaaagtacca cgagtattaa tgtcttgcat caaatcgtat
      361 ctcttcattt ctaatgctaa tgtcggcgac aggttgatgg cagatgcatt attgattaaa
      421 atatctatac ctccaaatgt ttctacggct ttgtttactg catttattac ttcagcttca
      481 tcacgtatat cacatttcac agccaaagct ttgcctcctg ctttttccat atctgctgca
      541 gcggtaaaaa ttgtacctgg aatttttgga ttaggttctg tacttttagc agcaatgacg
      601 atatttgcac cttcttgagc taaacgcata ccaattgcgt gtcctatgcc acgagttcct
      661 cctgaaataa aaacagtttt atttttaaat gttgtcatat tttgttattt taattacaac
      721 aagataccat attttttgtt ctgacatata ttgtattccc atttttttga gttatattta
      781 attatcattt attgcttttg cttatgaaag attttgatgt tattattttg ggagctggac
      841 ctggtggcta tgttgccgca attcgattgg cacaattgag ctataaagta gctattgttg
      901 aaaaagaaaa tttaggtggc atttgtttaa attggggttg cattcctaca aaagctttat
      961 taaaatcagc tcatttattc aaagaaataa aatcggctca tcaatatgga atatccattg
     1021 atgaagtaaa agctgatttt actaagatta ttgaaagaag cagaaatatt gcaaatgaaa
     1081 tgcaaaatgg cattcaattt ctgatgaaaa aaaacaaaat tgaagtaata aaaggttatg
     1141 gaaaaatact gccgggcaga cgtgtggaag ttactaataa tgaagctcaa atattgattt
     1201 ataaaagtga tgctatcata ttagctacag gaagttctcc aaaaaatata gcttcattac
     1261 ctattgacca tcaatatatt attgattaca aagatgcaat gtctatcaaa aaacaaccca
     1321 aagaattgat aattattggt gcaggtgcaa taggtgttga atttgcttat ttttatcaat
     1381 ctataggtac acaagtaacg ataattgaat tttctgaaca tattttgccg aatgaagatg
     1441 tagatgtttc cttagctttg aaaaaaagtt tagaaaaaca aggtattaaa atttacactt
     1501 cgtcaaaagt attaaatagt aaaattgaaa ataatgaagt tgtgctattg gtaaatacac
     1561 ctgaacaaga aattattttg aaatctgaat gtgttttgag tgctgtgggt attgctgcaa
     1621 atttagataa tattggtttg gaattgtgta atattgaaac cgagagagac aaaattaaaa
     1681 cgaatattta ttgtcaaaca aatgttgatg gagtgtatgc aatcggcgat attattggag
     1741 gtcaagcatt ggcacatact gcgagtgcag aagcaatcat tgctgctgaa cattttgcag
     1801 gtttatcaac tttgcctatt gattataaca atgttccgtc ttgtgtatat tgctcgccag
     1861 aaattgcttc agttggttat actgaagtac aattaattaa aaataaaata gactatttag
     1921 taggtaaatt tccatttagt gcctcaggaa aagcaaaagc cataggagaa accaaaggtt
     1981 ttgtaaaagt attggttgat aaaaagtatg atgaaatttt aggcgttcat attattggta
     2041 caaatgctac tgaaatcatt gaagaaattg tattggctag aagtttagaa gcaacaggaa
     2101 aaagtatatt acacagtatt catccacacc ctacgataaa tgaagcgata aaagaagcct
     2161 ttgaaaatgc ttacaagcag tctattcact tatagtttat ttttaatatg cagatacaaa
     2221 atgccaaata acaatgcctg cacataccga aatgtttagc gaatgcttgg ttccaaattg
     2281 tggtacttcg atacaagcaa cactttcatt gataaattca taagaaacac catctacttc
     2341 attgcccaat actaaagcat attgcttatt atcttcaatt ttaaattcat taagcatcgt
     2401 agaatgatca ctttgttcaa tagataaaat agtgtaattc ttttctttta gatattgaat
     2461 acaatcttct gttgtagaaa aatatcgcca atccacagtt tcagttgcac ccaaagctga
     2521 tttttgaatt tcacgattgg ggggttgagc agtgatgccc actaaaataa tttcttcgac
     2581 taaaaaagca tcagaagtac ggaatacaga acctacattc atggcactac gaacattatc
     2641 taatacaata actataggat ttttgatgct ttctttaaat tcatctcttg aaattctatt
     2701 aagttcatct aattttagtt tccgcatggc tttaaattaa aaattactat tgcaagaaaa
     2761 gaaaaagatt agagatatta atccattttc ggatatttaa acatttcata ctaaatagct
     2821 acactcctat tgatttggaa tttcaatttt aattttggaa ttttgtatta tgaaattgga
     2881 tattttagtt tttgcagctc atcccgatga tgctgaatta gcttgtggcg gaacgattgc
     2941 taagcatgta aaaaatggct ataaagttgg cataatagat ttaacgaatg gcgaattaag
     3001 ttcacgtggc aatgttgatt tacgaagaaa agaagctcaa aaagctgcag aaattttagg
     3061 gttatcagtt cgagagaatt taggttttag agatggtttt tttgaaattg atgaatatca
     3121 tttgcttgaa attgtaaaaa aaattagaca atatcaacca gcaattgtat tgtgcaatgc
     3181 catagacgac agacatcctg accataaacg tggtggcgat ttggtggagc gtgctttttt
     3241 tctgagtgga ttggttaaaa ttgaaacgga aataaatgga gttgctcaat ctatttggag
     3301 acctaaaaag ttgatacatt atatccaaga aaaatatatt aaaccaagtt ttgtagtaga
     3361 tatttctgat tttttagagg ttaaatttaa aagtattgaa gcctatactt cacaatttta
     3421 tcaaaaagat agtgatgaac cttatactaa aatttcagga gaaggctatt ttgaattttt
     3481 aaaatctaga gctaaagaat taggtagtca atctggctat gactatgcag aaggatttgt
     3541 aagcaaaact ttaattggaa tagataatat tattgatatt gaataaaatt atatggtcac
     3601 aaatggagtt tttttagtta gtgatgtgta aacctatttt aggacaagaa ataattaact
     3661 atccaattta tttatcaagc tttctaattg agtatttatt ttttgaattt gcttatagct
     3721 atatgcatca ttagccaaaa attcatcaat aattgttttg atatttttat tagacataat
     3781 cttttcttga atcaactgaa ttactaaatt gtttttccaa ctaagtagtt gttgtttgcg
     3841 tatagactca atatttactt tagaaaagta actttcgata tattttaaag tattatcaat
     3901 cccaatcttt tcaatagatg aagtttgtaa aacaatatta ttccattctt tattttgata
     3961 attcaataaa ggcaaaacac tttttaattg tgcaacacta atttttgctt gttgctcatt
     4021 tgctttgtct gctttgttga taataataat atcagccaac tccattacac cacgtttaat
     4081 accttgcaat tcatcgccag aatttggtaa aaccaacaac ataaataaat cagtaagatg
     4141 tttaatttct atttcagatt gccccacacc taccgtttca ataagtatat aatcaaaacc
     4201 aaaagcctca cataacaata tagtttgata tgtattttga gcaattccac ctaaataatt
     4261 actacttgca gttggacgta taaaaacatc tttatggtaa gcaatttcgt acattctagt
     4321 tttatcgcct aaaatgcttc cttttgtaat tgtactactt ggatcaatag ctaaaattgc
     4381 taaagactga gaattagcaa taatttctgc accaagcgca ttgataaatg tactttttcc
     4441 tacaccagga gaacccgtaa tgccaattct cttagattgt ttttcttgtt ttaaacaata
     4501 ttcaatcaat tgattagcta caacacgatg agctggtaca gtactttcta ttaaagtaat
     4561 agctctactc aacacaattc tgtctttatt gtttatgcca tcaatataat atgataaagg
     4621 ttctatcaag tgtcgctgat tgattgatta tttattaact tttacctatt tcccattact
     4681 caactcacga aatgctaacc acgccatcaa accagcacca atttctaaac aattttcatc
     4741 gacatcaaaa gttggtgtat gaatagatga agtgatacct ttttcaatat ttcctgttcc
     4801 taatctataa aaacatgctg gtacaacttg agtgtaataa ctaaaatctt ctgcggtcat
     4861 acgaatgggc aactcttcta cattttgctc tcctaaatat aatttagcat ttgcaatcgc
     4921 tttttgggta atttcttcat cgttttttaa gaatggatag ccatgagcta tatcaatttc
     4981 aatttctgca cccatacttt cagcaatact agtggctatt tgaacgattt tgattttcgc
     5041 atctgctctc cacttttcat cgaaagttct aaaagtacct tccattttta cttcttctgg
     5101 aattacatta gtagcaccat tggcaataaa tttccctatt gtcaaaactg aaggcataat
     5161 aggattagca tttctactta caatttgttg caaagcaatg acgatatgcg aagctatcaa
     5221 caccgtatca acagcaagat ttggaacagc accatgtccg ccatttcctt ttattgtaat
     5281 aaaaatttca tcacaacttg ccatcgaaat gccacttcta aatgctactt ttccagtttc
     5341 taattgtggc aaaacatgct ccccaaaaat cttttcaacc ttaggatttt ctaaaacacc
     5401 ttcttgaatt aatatcgatg caccaccagg taatttttct tccgctggtt gaaaaataaa
     5461 tttaatttgt ccttcaaaat tatctttgat ttcatttagt actttcaatg cacctattaa
     5521 acaagtcgta tgtacatcgt gtccacaagc atgcattttt ccttcattga cagagcaata
     5581 gctgacttta tttttttcgt gaatgggtaa tgcatcaata tcggctcgta aagctatcgt
     5641 ttgttggtct ggattatttc cttttattaa tcctactatt ccatgtcctc caatgtcttt
     5701 ttgataagtg atgcctaatt tatctaattg agtagcaata aattttgctg tttctttttc
     5761 ttcaaaactt aattcaggat gtgtgtgtaa atgtcttcta atttcaatca attgagtaga
     5821 aaattcattt gctgattgtt taattctatc tataatactc gttgtcatat atcaacaaaa
     5881 ttacaaaaaa taaaatgtat caatgccact aaataaaaaa gctttccaaa atttggaaag
     5941 cttaatttaa tatttttatt aaaaattaaa actgaaaatc agcatctgta agacttacac
     6001 caatttttaa gtttttcatt tcataagttt catacattcc ctctgcatct tccattcttt
     6061 ggtataatgg taaatatgtt tttttatcta aataaataat tgttttttta gcataagaag
     6121 aaggaatttt aatcttttgc ccaggtgtta attcgtcttt agataagccg tttaattctt
     6181 taattttata ctcagggata gctaattgat cacctaattt ccaaactgaa gtttgagttg
     6241 gtgtaacagt gtgcagaata attttataat cagggtcatt gatttcaatt ctatagcaat
     6301 ctttaccatc ataatttaca cttcctgtaa ttttagcaaa tttatctaaa tcttgacctt
     6361 ttcttgcgtc aatactcgtc atcaaaacct ttctaattct gccaaatcca gctctatcaa
     6421 ttgtttgatg ttgttctttc atcaataaac catttgtcaa ttcaagattt aatgtaaatc
     6481 ctttttttac ttttactttt gattgaactg atggaatata aactaattga gctttttgcg
     6541 gttcaataga attagcataa gttttcttta ctggtgattc ctgaatttta aattggatgt
     6601 atgaagaaat tacttttccg cctttaaatc tttctttaga atagaaatca taagaacaag
     6661 ttttaacagc tttcattgca gcatctgcct tcgctacaat atcttcaata gtttcggcaa
     6721 agatgtttga agtaaaaccc atcactccaa tcattaatgt caataagaat gtttttttca
     6781 ttttctaata aattattttt ttgtttgaga attttatatg accgtaatat tacaaaaact
     6841 ttgccaaaaa ttattgtact aaacaatttt ctttttctaa aaacttagta aaatgaaatt
     6901 gttctgtatt ttttatcatt tttttaacaa tttcatctaa acacaggttt ccaatgctac
     6961 cttccaaagt atgtttaagt tcattaggtt tttcataaga accattttct actttttgtc
     7021 ctacttctac atacgcatct cggaatgatt gaccttgatt gactaactca ttaatactat
     7081 ccacagtaaa aagatattta tacctatcat cttctaaaat ttgttcttta ataataatat
     7141 attccaacat cgtattggtc atttttatac aatcaattag agtatcaaaa ctatatagat
     7201 aatcttcttt tataatttgt aaatcacgat gataacctga aggtaaattt ccagttatca
     7261 tagaaatctg cgtaggcaga tttattaatt tattgcattt tgcacgaatc aattcaaaaa
     7321 catcaggatt ttttttatgc ggcattattg atgagcctgt cgtcaaaaca tcaggaaaac
     7381 taataaaacc aaaattttga ttgatataca atgttgcatc catcgccaac tttgctaaag
     7441 atgatgctat gtttgccaat gcactactta caaatttttc cattttacct ctgttcattt
     7501 gagcatagac aacattataa ttcaaggatt caaaacctaa taattctgtt gttcttattc
     7561 tatttaaagg aaatgaactt ccatatccag cacctgaacc taatggattt ttattaacaa
     7621 gagcataagc accatataac gaattaatat catctgaaag actttctgca taagcggaga
     7681 accataaacc aaaagaactt ggcattgcaa tttgcaaatg tgtatagcca ggcaaaagta
     7741 catctttatg cttttctgcc aagccaatta aggtatcaaa aagaatttgt gtttgctcta
     7801 ctgtttcttt aatttttgct ctcgaatata gcttaatatc taataaaact tggtcattac
     7861 gacttctacc agaatgtatt ttcttaccca aatcacctaa tttctgtgtt aatctatact
     7921 ccacttctga atgaatatct tcgctgcttt catctaaagt aaactctcca gcttcgatag
     7981 tgttataaat attttttagc tctttatgaa ttggcactaa ttcttcttta ggtaacaatc
     8041 caacctctgc caacatctga gtatgtgcca atgaacctaa aacatcatat tttgccaaaa
     8101 ttaaatctaa ctcacgatct ttgccaactg taaaagtttc caccaatttt gataattccg
     8161 aattatctat ttcttttttc tgccaaattt ttgccacagt ctataattta attagaataa
     8221 gttacaaatt tacgatttct tttgttttta aagacagaat atacaaaact aaaatacgtc
     8281 tctatttcgt agaaaacatt acaatttaat agctgtcaat aaaaattagc attatttcct
     8341 tagctttatc attttttatt attatcaacg attactaaat ggaaaaatta tatctattcg
     8401 attcttttgc aatgattttt agagcttatt ttgctttaca aaaaaatcca ttaatcaatt
     8461 caaaaggtca aaatgtttct gccattactg gttttttaaa tacactttac gaaataatca
     8521 ctaaatataa accaacgcat attgcatgtg cattcgatag tacagaaccc actatccgcc
     8581 acgaagcatt tgaagcatat aaagctaatc gttcagaaac tccagaagat ataatatttt
     8641 caattccata tattaaagca attattaaag gctttaatat tccaatttta gaagtcccag
     8701 gttatgaagc tgatgatatt attggtgcaa ttgcaatgca agcagaacaa cataatattg
     8761 aaacttatat tgtaactatg gataaagatt tgggacaatt agtttcagat aaaacgttta
     8821 ttcatagacc gccttatatg ggaaaaccag ctcaaattta tggtgtacaa gaaatttgtg
     8881 aacgttggga aatcgataac ccaaaacaac ttattgatat tttaggctta atgggtgatg
     8941 cagtagataa tattcctggt gttgctggta ttggagaaaa gactgcacaa aaattaatta
     9001 aagattttgg aagtattgaa ggaatctatg aaaatataga acagattaaa ggcaaactaa
     9061 aagataaatt agttgaacaa aaagaacagg cttatatgtc taaaagttta gctactgttg
     9121 aactgaatgt accagttgag tttcatattg aaacttttaa aattcaagag aaaaatactg
     9181 ttgaattagc tgaaattttt gctgaattag aatttagaac gcaagccaaa agaattttag
     9241 gtgatagtta taacttggta gaagaaaaaa ataaagcaag tatcaataac aatcagcaat
     9301 ttgatttatt ttcttcgata agcgaaacta cttcagcaac tgaaaatata tctaataata
     9361 aaaatttata caacactgaa catcaatatt cattaatcga ttccgaaaac aaactagctg
     9421 aactaataca aaaaattgct aaagaaaaat caatttgttt tgatacagaa actacatctt
     9481 taaatacttt tgaaagtgaa ttagtaggaa tcagcattag tctcaaagaa aatgaaggct
     9541 tttatattcc ttgtcctacg aacaaacaac aaacacaagc cataattgaa cagcttcgac
     9601 ctattttaga aaatgaaaat attactaaaa ttggacagaa tatcaaatat gatttattgg
     9661 ttttaaaaaa ttacaatata gaattaaaag gcatactttg ggataccatg ttggcacatt
     9721 acctgattga accagatgct cgacacggaa tggatatttt ggctgaaaat tatctgaatt
     9781 atcaaactat ttctattgaa gaattgattg gtaagaaagg aaaaaatcaa ggtaatatgc
     9841 gtgatgttcc aattgaaaaa atcaaagatt atgccataga agatgctgat attactttac
     9901 aacttcaaca aattttcaaa aaggaaatag accaaacgca tttagaaaat ttattttata
     9961 atgttgaaac taaattaatc acagtattgg cagatatgga gcgtgagggt atcaatgtag
    10021 atgttccttt tttacagcaa tattctagtg aaattcaaga agaattaatt caattacaag
    10081 atacaattta taaaaagagt ggcacaactt tcaatattga ctctcctaaa caattaggag
    10141 aaatactatt tgaacatcta aaaattcctt atcaaggaaa aaaaacaaaa actggacaat
    10201 atagtactga cgaagctact ttacagaagt tttttgacaa acatcctatt atacaatcta
    10261 ttttggatta tagagaattt acaaaattaa aatcgactta tgttgatgca ataccaaatt
    10321 taattaatcc tttaaccaat agaatccata cgacttataa tcaaacaagt gtagcaacag
    10381 gaagattaag ttctattaac ccaaatttgc aaaatattcc tattcgtact gaacgaggaa
    10441 aacaaatcag aaaagcattt atacctagag atgaaaacta tactttatta tcagcagatt
    10501 attctcagat tgaattaaga attgtcgctt ccataagtgg agatgaaaat atgatggaag
    10561 cattcagaca acagcaagac attcactccg ctacagcagc ccgagtattt aatgtaagtt
    10621 tagaagaagt cagttcagaa atgagaagaa aggctaaaat ggtcaatttt ggtattattt
    10681 atggtatatc tgcatttggt ttggctcaaa gattaggcac aggaagaaat gaagccgctg
    10741 aattaattga ggaatatttt aagcaatatc caaaaattaa aaactatatg gacaatacta
    10801 tactttttgc acaacaaaat ggctatgttg aaaccttatt aaaaagaaga aattatatca
    10861 gagatataaa ttcagaaaat tatactatcc gaggttttgc agaaagaatt gcgattaatg
    10921 caccgattca aggttcggca gctgatatga taaaagtagc aatgattgat attcatcaac
    10981 aactaaaaag tctaaaatgt cattcaaaat tactattaca agtacacgat gaattaatcc
    11041 ttgatgtaca taaatctgaa aaagaaatta ttgaaaagat agtaatatct tcaatgaaaa
    11101 acgctttacc attaaacatt cctgttgagg tgagttgtgg ttggggaaac aattggttag
    11161 aggctcatta attaaataaa taaggtcaaa ctactaataa aacttactaa catcgttaaa
    11221 gaacgatgtt agtaaatact tttccacaaa acaatacttt taaggagttg tgtggaacac
    11281 ttttttttaa gtcgcattaa aattcatcta cttttgagat tgaattagtg taaaaacttt
    11341 ggttttgaaa ctaccaaaat ttgatatttt atgaaaagta gattattttt atttgttttt
    11401 attattttaa gtttctttgg attctctgct aattgggatg ctacttatat ctccaagtta
    11461 ttggcaaaaa aagagtatga caaaataatt aattactaca aaaaagagta cacgcaaaag
    11521 ccagagtatg cttttaaaat tgctgatgta tatgccaaaa agaaagatta tgcaactgca
    11581 atttcttggt atcaaaaaga accacattta ttaaaagaaa ctaaagtgaa cctcataaat
    11641 ttggcaaaaa cctataagga aatgggtgat tatcaaaaag cgttagatac ttatattact
    11701 tatgctgctg aatcaggcga tgctactaaa gtattaaaag atgctcaatt atgtgaaaag
    11761 ttaattcgtg ctagttcatt tactgataat tatttaatcg aaccatacac tttcaataca
    11821 gcagtaaatg aaattaattt aagtattctt agagctaatt taatttacct tcaaagggat
    11881 tttgagaatg aaaaaaggaa aaatgattct cctaattctg ttactttgca agcacaaaga
    11941 atgtttgaca aatggaatga acctataaaa attatccaac aagaggaaac gaataaagaa
    12001 tatatcaagt tttcatttac taaagatggt aacaaagttg ttttctctgc aaaaccagca
    12061 accttattgc ccaataaaaa agaaagcaat aaaaaaccaa aattatatga tcaaattttt
    12121 gttgcagatt tcttaggtgg acaattattc aatataaaat catttcctta taattctgaa
    12181 gaatattctt gtgcaaatcc aagttttaat aatgatggta ctaaaataat tttctcttca
    12241 gatatgagta ataaaactga tggttttgat atttatgaaa gtgaattaat cgaaggaaaa
    12301 tggactaaac ctaaaaattt gggcaactta ttgaactcac ctaaaaatga taatcaccca
    12361 ttctatttaa aagagaagaa caataaagaa tatttatatt tttcatctga cagagaagat
    12421 ggatttggtg gttatgatat atatcgtgca gaatatagtg atggactttg gcaagatgtt
    12481 caattattac cagcaccaat caacacagca ggtaacgaag atagttatac ttatgattta
    12541 gaaacacaaa ctgcttatgt aagttcagac agaagcgata gcaaaggtgg ttttgatata
    12601 tacagaatta aaccactcaa tttattatta tttattaccg taaaagataa tgatactaaa
    12661 gacccattag gatttgcgtt tttagaatta tttgaaaaaa ataatcaa
//
LOCUS       DAOQCW010000073        12241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1930_length_12241_cov_5.909650, whole genome shotgun sequence.
ACCESSION   DAOQCW010000073 DAOQCW010000000
VERSION     DAOQCW010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12241
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1930_length_12241_cov_5.909650"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PLS16_07645"
     CDS             <1..252
                     /locus_tag="PLS16_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNJ60991.1"
                     /translation="SFTFVIKTPPASALLLEASKAKKGSAQPNRNKIGAVSWAQVKEI
                     AEIKMPDLNCFTVESAMRMVAGTARSLGITVDGTAPWEA"
     gene            335..1030
                     /gene="rplA"
                     /locus_tag="PLS16_07650"
     CDS             335..1030
                     /gene="rplA"
                     /locus_tag="PLS16_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNJ60992.1"
                     /translation="MKVSKNRKKVNEKLEKDKVYTLEQASALIKELNTTKFNSSVDLH
                     IRLGVDPRKPDQALRGTVSLPHGTGRSKTVLVLCPADKEEQAKAAGADYYGLDEYLEK
                     IAGGWTDVDVIIATPSVMPKLGRLGKILGPRNLMPNPKSGTVTENVGDAVTEVKKGKI
                     AFKIDKFGIIHTSVGRISFEPNQIFDNAKEMMTTISKMKPSSAKGIYFKSIYLATTMS
                     PALQIDVKSVPGI"
     gene            1055..1576
                     /gene="rplJ"
                     /locus_tag="PLS16_07655"
     CDS             1055..1576
                     /gene="rplJ"
                     /locus_tag="PLS16_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNJ60993.1"
                     /translation="MTRAEKQATIDQLKNTFENSEYFYLTDTSGLNVEDFGKLRRICF
                     EKGITIQVAKNTFIRKALDSCGKLSNEIEPALHGTTALMISETGNLPAKVIKEFRGDK
                     EKPALKAAYIDSAIFSGDNQLAALVALKSKNELIGDVIGLLQSPAKNVISALKSSGQT
                     IAGLVKTLEERNN"
     gene            1604..1981
                     /gene="rplL"
                     /locus_tag="PLS16_07660"
     CDS             1604..1981
                     /gene="rplL"
                     /locus_tag="PLS16_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNJ60994.1"
                     /translation="MADIKALAEQLVNLTVKEVNELAEVLKSEYGIEPAAAAVAVAAP
                     AAGGGAAAAEEKTSFDVILKDGGAQKLGVVKVVKELTGLGLKEAKELVDGAPKAIKEG
                     VSKDEAESLKAKLEEAGASVELK"
     gene            2075..5905
                     /gene="rpoB"
                     /locus_tag="PLS16_07665"
     CDS             2075..5905
                     /gene="rpoB"
                     /locus_tag="PLS16_07665"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNJ60995.1"
                     /translation="MSKSNSKNLTSSGRVNFGKINNQYDFPNLLDIQVKSYKDFLQLE
                     TTADNRKDEGLYNVFAENFPINDIRNIFLLEFLDYYVDPPRYSIEECIERGLTYSVPL
                     KAKLRLSCNDEEHIDFQTIVQDVFLGNIPYMTDKGTFIINGAERVVVSQLHRSPGVFF
                     GMSFHPNGTKIYSARVIPFKGAWMEFATDINNVMYAYIDRKKKFPVTTLLRAIGFDTD
                     KDILNLFGLSDEIQVNKKNLEKNIGRKLVARVLKSWMEDFVDEDTGEVVSIERNEIIL
                     ERDTILDEKNINLILETDVKTIILEKENIDEDYSIIFNTLQKDTSNSELEALQHIYKQ
                     LRGTEPPDDDTARGIIEKLFFSDKRYDLGFVGRYKINKKLDLDIKDDTIILTKEDIVS
                     IVKYLIQLINQKAEIDDIDHLSNRRVRTVGEQLYAQFGVGLARMARTIRERMNVRDNE
                     VFTPIDLINARTLSSVINSFFGTSQLSQFLDQTNPLAEITHKRRISALGPGGLSRERA
                     GFEVRDVHYSHYGRLCTIETPEGPNIGLISTLCVHAKVNEMGFIETPYRRVKDGKLLN
                     KVEFLTAEEEDINMIAQSNVTVSDKGELVDEKIRCRFQDDFPVIDRNEDEFNTINYMD
                     VAPNQIVGVSASLIPFLENDDANRALMGSNMQRQAVPLMRTESPIVGTGIEAKVAHDS
                     RVLVNAERSGVVEYVDANEIRIRYDRTDNERLVSFDEDVKVYKLTKFARTNQATCINL
                     IPIVRKGDKIKAGQVLCEGYATQNGELALGKNLLVSFMPWKGYNYEDAIVINEKVVRE
                     DIFTSIHVEEYELDVRDTKLGEEEFTNDIPNISEDITRNLDENGIIRVGAKIKEGDIL
                     IGKITPKGESDPTPEEKLLRAIFGDKAGDVKDASYKAPPSTEGVVINTKLFQKARKDK
                     NSKIREKAELEKAEKVHIQNLEEIKRVLVDKLMTLLGGKTTNGIKNIFGEEAVPKGTK
                     FTEKLFGSLNYQNIDTSSWTKEDETNDLIKRLVHNYLIKHNEEKGRFLRDKYAITVGD
                     ELPAGVLKLAKVYVAQKRKLKVGDKMAGRHGNKGIVAKIVRQEDMPFLEDGTPVDIVL
                     NPLGVPSRMNIGQIYETVLGWAGKQLGIKFATPIFDGASVEDIEDFVTEAGLPKLGHA
                     YLYDGETGDRFDQRSTVGVIYMIKLAHMVDDKMHARSIGPYSLITQQPLGGKAQFGGQ
                     RLGEMEVWALEAYGASNILQELLTIKSDDIQGRAKAYEAIVKGDNLPEAGVPESFNVL
                     VHELRGLALDLDFE"
     gene            complement(5954..6571)
                     /locus_tag="PLS16_07670"
     CDS             complement(5954..6571)
                     /locus_tag="PLS16_07670"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNJ60996.1"
                     /translation="MTISLQDISKRYGLEWILKKVNLELITNNQYAVIGPNGSGKSTL
                     LKIIANGIVPTQGKITYSIDNNITPSDFLINKNIAFTAPYISILEEMSLEEMYIFHSK
                     LHAMYCKDLLHFLDIIELQKHRNKYIKHFSSGMKQRVKLALAILSKTEVLILDEPSSN
                     LDEKATQWYLNLVQKYMQDRIVLIGSNQEREYNFCTHQINVLDYK"
     gene            complement(6575..7153)
                     /locus_tag="PLS16_07675"
     CDS             complement(6575..7153)
                     /locus_tag="PLS16_07675"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HNJ60997.1"
                     /translation="MNTISDIIQKRRTIDPDCFNGKIVPKVIIDKMLAATNWAPNHGH
                     TEPWDFIIFEGHEKCKEFGVIHANLYKANNTPEHFLQKKYDKLLHRADNCSHVIICTN
                     NRGNSKNIPDLEEICATSAAIQNLLLVAQENGVATFWSTGGLTHHDAFKAYFGYSEKD
                     TVLAVIYIGYTDVEIPEGKRNSDFSNKVKYYQ"
     gene            7339..9201
                     /locus_tag="PLS16_07680"
     CDS             7339..9201
                     /locus_tag="PLS16_07680"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HNJ60998.1"
                     /translation="MAEVQYTEDNIRTLDWKEHIRLRPGMYIGKLGDGTSPDDGIYVL
                     LKEVIDNSIDEHVMGFGKQIDVVIKDKTVKIRDYGRGIPLGAVIDCVSKINTGGKYDS
                     RAFQKSVGLNGVGTKAVNALSTYFKVESVRDGEKKAAEFSVGELTTDFKIEKTNDRNG
                     TTFTFVADNTIFKNFNFVEDFVEEQLWNYAYLNAGLTINYNNKKFVSKEGLKDLLSKK
                     TNESELRYPIIHLKGDDIEFALTHGNQYGEEYYSFVNGQYTTQGGTHLQAFKEAVVKT
                     IREFYKKSFEASDVRQAIVGAIALKVQEPVFESQTKTKLGSQNMWLPDGPTLRTFVND
                     FVKEKLDNFLHRNQDIADALLKRIQQSERERKDLSGIRKLANERAKSANLHNKKLRDC
                     KVHYTDEKNEKHRDTMLFITEGDSASGSITKARNPELQAVFSLRGKPLNCYGLTKKIV
                     YQNEEFNLLQHALNIEEDIDNLRYNHVVIATDADVDGMHIRLLIMTFFLQFFPDLVKK
                     GHLFVLETPLFRVRNKQETIYCYSEQEKQKAIDKLKGKPEITRFKGLGEISPSEFEEF
                     INEDIRLEPVLLKQNQSIEQLLEYFMGKNTPERQEFIINNLKVEKDIVEEALLS"
     gene            complement(9203..11308)
                     /gene="feoB"
                     /locus_tag="PLS16_07685"
     CDS             complement(9203..11308)
                     /gene="feoB"
                     /locus_tag="PLS16_07685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNJ60999.1"
                     /translation="MAVQKNVAKIALIGNPNAGKSSVFNQLTGLQQKVGNFTGVTVDK
                     KTGFSQLDKNTKASLIDLPGTYSLYPNSADEKIVLDILLDKNNKDFPELILYILDASQ
                     IERHCLLLTQLIDLKFKVVVAINMIDVAKKNHIKIDSKQFEKTFGVPAIPINARSGDN
                     FDLLKKTLAEQLNTPADLKIFYPIENEILNEIRAVIPNEDTYRSWLIFQHSKQVHFIS
                     TEQRLDLNNLKTKHQFNSITEQFDEILDRYNAFTPDLPKIIQRQLNSSNTFTDKLDNI
                     LTHKFWGVLIFFALMLLVFQAMFNWSTIPMDFIDAQFTRMAGNIANNMPDNLLTRLLT
                     EGIIPGIGGIVIFVPQIFLLFLIISILEEVGYMSRAVYLFDSLMQRFGLNGRSIVALI
                     SGGACAIPAIMSTRTISNPKERLITILITPFISCSARIPIYALLIAFVVPSTVVFGFL
                     NLQALVFMGMYLLGVVAALGSAYILHKILKTTDTSFLSLDLPEYKIPYWKNVMMEVNN
                     KVQAFVFGAGKVIIVISIVLWFAASFGPNLANKKIQFAQEAAQMNLSEQESSEYIASK
                     TLENSYAGIAGRVIEPVIKPIGFDWKIGIAIITSFAAREVFIGTMSTLYSIGEDAGES
                     TIREKLGKQINPETKQKVFTVATSLSLLVFYAFALQCMSTMAVVYRETKTWKYPIYQF
                     IGMGLFAYIASFIVYHLFK"
     gene            complement(11310..11573)
                     /locus_tag="PLS16_07690"
     CDS             complement(11310..11573)
                     /locus_tag="PLS16_07690"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HNJ61000.1"
                     /translation="MYSIKTKSKLLVAVKNITNLRKGEISSIQNFTDKILGSKLISMG
                     ILPNASVELMRKTLFGSTYYVRVNNCCNIALRKQEALSIIISE"
     gene            11769..>12241
                     /locus_tag="PLS16_07695"
     CDS             11769..>12241
                     /locus_tag="PLS16_07695"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNJ61001.1"
                     /translation="MKKLSMLILLFFVLGYTHNLKAQNSDWIDIENAIAKTDAQHQFI
                     LVDIYTDWCGWCKKMDQTAFADPATLANLKNNFVLVKLNAEGENPINFNGKTYNPVPT
                     GNRKTSQLAIDLGSVSGRIGYPTLVVLDSKGNKLQAFPGYKDADTINQLVKYYVSG"
BASE COUNT         4361 a         1715 c         2167 g         3998 t
ORIGIN      
        1 tcttttacgt tcgttattaa aactcctccg gcttccgctt tattattaga ggcgagcaaa
       61 gcaaaaaaag gctctgcaca acctaatcgt aataaaatcg gagcagtttc ttgggcacaa
      121 gtaaaagaaa tagcagagat caaaatgcct gacttaaatt gctttactgt cgaatcagcg
      181 atgagaatgg ttgctggaac agcaagaagt ttaggtatca cggttgatgg tactgcacca
      241 tgggaagcat aatttattag ttgttacgct aaattatgtt tcgagagagt tacactttgg
      301 tgtgttacgt tattatctca ttaaaaatta aaaaatgaaa gtatcaaaaa acagaaaaaa
      361 ggttaacgaa aagttagaaa aggacaaagt ctatacctta gaacaagcat ctgctttaat
      421 taaagaatta aacactacaa aatttaattc atctgtagat ttacacatta gattaggtgt
      481 agatcctaga aaaccagacc aagcattgag aggaacagtt agtttacctc atggtacagg
      541 tagatctaaa actgtattag ttttgtgtcc tgctgataaa gaagaacaag ctaaagctgc
      601 aggagcggac tattatggtt tagatgaata tttagaaaaa attgctggtg gttggacaga
      661 tgttgatgta attattgcaa ctccaagtgt aatgcctaaa ttaggtcgtt taggtaaaat
      721 attaggtcct agaaacttaa tgcctaatcc taaatcagga acagttactg aaaatgtagg
      781 tgatgcagtt acagaagtta aaaaaggtaa gattgctttt aaaatagata agtttggtat
      841 catccatact tcagttggaa gaatttcttt tgaaccaaat caaatttttg ataatgctaa
      901 agaaatgatg acaacgattt caaagatgaa accatcttct gcaaaaggaa tctacttcaa
      961 aagtatctat ttagcaacga ctatgagtcc tgctctacaa attgatgtaa agagtgtacc
     1021 tggaatttaa tcttaacagc taaaaaaaag gaaaatgaca agagctgaga aacaagcaac
     1081 gatagatcaa cttaaaaata cttttgagaa tagtgaatat ttttatttaa cagatacttc
     1141 aggattgaat gttgaagatt ttggtaaatt aagaagaatt tgcttcgaaa aaggaattac
     1201 tattcaagtt gcaaaaaaca catttatcag aaaagcctta gatagctgtg gcaaattatc
     1261 aaatgaaata gaaccagctt tgcatggtac tactgctttg atgattagcg aaacaggaaa
     1321 tcttccggca aaagtaatca aagagtttag aggtgataaa gaaaaaccag cacttaaagc
     1381 agcttatatt gattccgcca ttttctctgg cgataatcaa ttagcagcat tggttgctct
     1441 aaaatctaaa aacgaattaa ttggtgatgt aattggatta ttacaatctc cagctaagaa
     1501 tgtaatttct gctttaaaat caagcggaca aacaattgca ggattagtaa aaacattaga
     1561 agaaagaaat aattaattag taaacttttt aaatttaatt aaaatggctg atataaaagc
     1621 attagcagaa caactagtaa acttaacagt taaagaagta aatgaacttg cagaagttct
     1681 aaagtctgag tatggcattg agccagcagc agcagcagtt gcagtagcag ctccagcagc
     1741 aggtggtggt gcagcagcag ctgaagagaa aacatctttt gatgtaatct taaaagatgg
     1801 tggtgctcaa aaattaggcg tagtaaaagt agttaaagaa ttaactggct tagggttaaa
     1861 agaagctaaa gaattagttg acggtgctcc taaagcaatc aaagaaggtg tttctaaaga
     1921 tgaggctgaa tcattaaaag ctaaattgga agaagctgga gcaagtgttg agttaaaata
     1981 attagctcta tgaatatatg tgtctggcag tctttgtact gccatgacgc attttgtatt
     2041 gaaatgcatt tttgttaatt tatctttgta atacatgtct aaatcaaatt caaaaaatct
     2101 tacttcatca ggaagagtta actttggtaa aattaataac caatacgact ttccaaactt
     2161 attggacatt caagtaaaat cttacaagga ttttcttcaa cttgaaacca ctgctgataa
     2221 tagaaaagat gaaggtcttt ataatgtatt tgcagaaaac ttcccaataa acgacattag
     2281 aaatattttc ttattggagt ttttggatta ctatgtagat ccacctcgtt actctattga
     2341 agagtgtatt gaaagaggtt taacttatag tgtacctttg aaagctaaat taagactttc
     2401 atgtaatgat gaagagcata tagattttca aactatcgtt caggatgtgt ttttgggtaa
     2461 tatcccatac atgactgata aaggtacttt tataattaac ggtgcagaaa gagttgttgt
     2521 ttctcaatta caccgttctc caggtgtttt ctttggaatg agttttcatc caaatggcac
     2581 aaaaatttat tcagcaagag tcattccttt taaaggtgct tggatggaat ttgcaacaga
     2641 tatcaataat gtgatgtatg catatattga tagaaagaaa aagttcccag ttacgacttt
     2701 gcttagagca attggttttg atacagataa ggatatttta aatctttttg gtttatctga
     2761 tgaaattcaa gtaaacaaaa agaatttaga aaaaaatatt ggtagaaaat tagtagcacg
     2821 tgttcttaaa tcttggatgg aagactttgt tgatgaagat actggagaag ttgtttctat
     2881 cgaaagaaat gagattatct tagagcgtga tacaatttta gatgagaaaa atattaatct
     2941 aattttagaa actgatgtta aaacaataat cttagaaaaa gaaaatattg acgaagatta
     3001 ttctatcatt ttcaatactt tgcaaaaaga tacctcaaat tctgaattag aagcattaca
     3061 acatatctac aaacaattaa gaggtactga accacctgat gatgatactg caagaggaat
     3121 tatcgaaaaa ttattcttct ctgataaacg ttatgactta ggttttgtgg gtcgttataa
     3181 gattaataaa aaattagatt tagatattaa agatgatacg attatcttaa caaaagagga
     3241 catcgtttct attgttaaat atttaattca attaattaat caaaaagcag agattgatga
     3301 tattgaccac ttaagtaata gaagagtaag aacagttgga gaacaattat acgctcaatt
     3361 tggtgttggt ttagctcgta tggcgagaac tatccgtgaa cgtatgaacg tgagagataa
     3421 tgaagtgttt acgccaattg acttaattaa tgcaagaact ttatcttctg tgattaattc
     3481 attctttgga acatctcaat tatcacaatt cttagaccaa accaatccac tagctgaaat
     3541 tacacacaaa cgtcgtattt ctgcattagg acctggtggt ttatcaagag agagagctgg
     3601 ttttgaagtg cgtgacgtac actattctca ctatggtcgt ttgtgtacaa ttgaaacgcc
     3661 agaaggacca aatattggtt tgatttctac actttgtgtt cacgcaaaag tgaatgaaat
     3721 gggctttatt gaaacgcctt atagaagagt gaaagatgga aaacttttaa ataaagtaga
     3781 attcttaacg gcagaggagg aagatattaa tatgattgct cagtctaatg ttacagtaag
     3841 tgataaaggc gagttagttg atgaaaaaat tagatgtcgt ttccaagatg attttcctgt
     3901 tattgataga aatgaagatg aattcaatac gattaattat atggacgttg ctcctaacca
     3961 aatcgtgggt gtttctgcat ctttaattcc attcttagaa aatgatgatg ctaaccgtgc
     4021 tttgatgggt tcaaacatgc aacgtcaagc agttccatta atgagaacgg aatccccaat
     4081 tgttggaaca ggaattgaag caaaagttgc acacgattca agagttttag tgaatgctga
     4141 acgttcaggg gtggttgaat atgttgatgc taacgaaatc agaatcagat atgacagaac
     4201 ggacaatgaa cgtttagtaa gctttgatga agatgtaaaa gtttataaat taactaagtt
     4261 tgcaagaacg aaccaagcaa cttgtatcaa cctgattcca attgttagaa aaggggataa
     4321 gattaaagca ggacaagtac tttgtgaagg ttatgcgaca caaaatggag aattagcttt
     4381 aggtaagaac ttattagttt ctttcatgcc ttggaaaggt tataactatg aagatgcgat
     4441 tgtaatcaat gaaaaagttg taagagaaga tatttttaca tctatacatg tggaagaata
     4501 tgaattagat gttagagata caaaattagg tgaagaagaa tttacaaatg atattccaaa
     4561 tataagtgaa gatattacac gcaatttaga tgaaaatggc atcattagag taggtgcaaa
     4621 aatcaaagaa ggcgatatct taattggtaa aattacacca aaaggcgagt ctgatccaac
     4681 acctgaagag aaattattaa gagcaatctt tggtgataaa gcgggtgatg ttaaagatgc
     4741 atcttacaaa gctccaccat caactgaagg cgtagttatc aatacaaaat tattccaaaa
     4801 agctcgcaaa gataaaaact ctaaaattag agagaaagct gagttagaaa aagccgaaaa
     4861 agtacatatt caaaacttag aagaaattaa acgagtattg gttgataaat taatgacttt
     4921 attaggtgga aaaactacta atggcattaa gaatatcttt ggtgaggaag cagttcctaa
     4981 gggtactaaa tttactgaaa aattatttgg tagcttgaat tatcaaaaca tagatacttc
     5041 ttcttggact aaagaagatg aaacaaatga tttaatcaag cgtttagtgc ataactactt
     5101 aatcaaacac aacgaagaaa aaggtcgttt cttgagagat aaatatgcta ttacggtggg
     5161 tgatgagtta ccagctggag tattaaaatt agcaaaagtt tatgttgctc aaaaacgtaa
     5221 attgaaagta ggagataaga tggcaggtcg tcatggaaac aaaggtattg ttgctaaaat
     5281 tgtaagacaa gaagatatgc ctttcttaga agacggaacg cctgttgata ttgtgttgaa
     5341 tccattaggt gtaccttctc gtatgaatat cggacagatt tatgaaactg tattaggttg
     5401 ggcaggtaag caattaggta tcaaatttgc aacgccaatt tttgatggtg catctgttga
     5461 agatattgaa gattttgtta ctgaagctgg tttgccaaaa ttaggacatg cttatttata
     5521 tgatggagaa actggcgacc gttttgatca aagatcaact gttggggtta tctatatgat
     5581 taaattagca cacatggttg atgataaaat gcatgctcgt tcaatcggac cttactcatt
     5641 gattacacaa caaccattag gtggtaaagc acaatttggt ggtcagcgtt taggtgagat
     5701 ggaggtttgg gcattagaag cttatggagc atcaaatatc ttacaagaat tattgacaat
     5761 taaatctgat gatatacaag gaagagcaaa agcttatgaa gctattgtaa aaggagataa
     5821 cttgccagaa gcgggtgttc ctgaatcttt caatgtatta gtacatgaat taagaggtct
     5881 tgctttagat ttagattttg aataaaatct aaggtattca taaataaaaa tgttccgcaa
     5941 ttgcggaaca tttttattta tagtctaaaa cattaatttg atgtgtacaa aagttgtatt
     6001 cacgttcttg atttgaacca attagcacta ttcggtcttg catatatttt tgtactaaat
     6061 tcaaatacca ttgtgttgct ttttcatcta aattagaact tggttcatct aaaattagaa
     6121 cttcagtttt tgaaagtatt gccaaagcta attttacacg ttgtttcatg ccagaagaaa
     6181 aatgcttgat atatttattt ctatgctttt gcaattctat tatatccaga aaatgcagta
     6241 agtctttaca atacatagca tgtaatttgg aatgaaatat atacatctct tctaagctca
     6301 tttcttctaa aatagaaata taaggtgctg taaaagcaat atttttattg attaaaaagt
     6361 ctgaaggtgt tatattatta tctattgaat acgttatttt cccttgagta ggcacaatac
     6421 catttgcaat tattttcaat aaagtagatt ttccacttcc atttgggcca attacagcat
     6481 attgattatt tgtaattaat tctaaattta ctttttttag aatccattct aaaccataac
     6541 gcttgctaat atcttgaagg cttatagtca tttattattg gtaatatttt actttattac
     6601 taaaatcact atttcttttt ccttcaggaa tttctacatc agtataacca atatagatta
     6661 cagccaatac agtatctttt tcagaataac caaaataagc cttgaatgcg tcatgatggg
     6721 ttaaacctcc agtactccaa aatgtagcaa ctccattttc ttgagcaact aaaagtagat
     6781 tttgaatagc cgcacttgtt gcacaaattt cttccaaatc aggaatattt ttgctatttc
     6841 ctctattatt tgtacagatg atgacgtggg aacaattatc agctctgtgc agtaatttat
     6901 cgtatttttt ttgtaaaaaa tgctctggcg tattgtttgc tttatacaaa ttcgcatgaa
     6961 ttactccaaa ttccttacat ttttcatgac cttcaaatat tataaaatcc caaggttctg
     7021 tgtgtccatg gttcggagcc caatttgttg ctgccaacat tttgtcaatt ataactttag
     7081 gtacaatttt cccattgaaa caatctgggt caatagttct tcttttttgt atgatatctg
     7141 aaatggtatt cattttttaa actttttcaa agataagaag aatatataaa gtccaatttt
     7201 ttattcaata atttatttgt aaatagatta gtttttgtat tataatatct atcctaaaaa
     7261 cgtaaatgtg gataaatgag ctttactatt tttagctaat acaatacatt tgtaatttaa
     7321 gattttagac aaaagattat ggcagaagta caatataccg aagataatat acggacatta
     7381 gattggaaag aacatatccg tttgcgaccg ggtatgtata ttggaaaatt gggcgatggc
     7441 acatctcctg atgatggaat ttatgtacta ttgaaggaag ttatagataa ttcgatagat
     7501 gaacacgtga tgggttttgg aaaacaaatt gatgtggtta tcaaagataa aacagttaaa
     7561 attcgtgatt atggtcgagg gattcctttg ggtgctgtga tagactgtgt ttctaagata
     7621 aatacgggtg gaaaatatga ctcacgtgct ttccaaaaat ctgttgggtt aaatggtgtt
     7681 ggaacgaaag cggttaatgc tttatctact tattttaaag tagagtccgt gcgtgatgga
     7741 gagaaaaaag cagcagaatt tagtgttggc gaattaacaa ctgatttcaa aatcgaaaaa
     7801 actaacgata gaaatggtac tacttttact tttgttgctg ataatactat tttcaaaaat
     7861 ttcaattttg tagaagattt tgtagaagaa caattgtgga attatgccta tttgaatgcg
     7921 ggtttgacca tcaattataa taacaaaaaa tttgtttcaa aagagggctt aaaagattta
     7981 ttaagcaaaa aaacaaatga atctgaattg cgttatccaa ttattcattt aaaaggtgat
     8041 gatattgaat ttgctttaac acacggaaat caatatggag aagaatatta ttcatttgta
     8101 aatggacaat atacaacaca aggcggaaca catttacagg cttttaaaga agctgttgtt
     8161 aaaacaatca gagagtttta taaaaagagt ttcgaagctt cagatgttag acaggctatt
     8221 gttggtgcaa ttgcattaaa agtacaagaa cctgtttttg aatcacagac caaaacaaaa
     8281 ttaggttctc aaaatatgtg gttgccagat ggtccaactt tgagaacttt tgtaaatgat
     8341 tttgtgaaag aaaaattaga taatttctta catcgaaatc aagatattgc agatgcttta
     8401 ttgaaaagaa tccaacagtc tgaacgtgaa agaaaagact tgagtggcat aagaaaacta
     8461 gccaatgaaa gagcaaaatc tgctaatttg cacaataaaa aactaagaga ttgtaaagta
     8521 cattataccg atgagaagaa tgagaaacat cgagatacaa tgctatttat taccgaggga
     8581 gattctgcat ctggctcaat tactaaagct agaaatcctg aattgcaagc tgtttttagt
     8641 ttgcgtggaa agcccttaaa ttgctatgga ttaaccaaaa aaattgtcta tcaaaatgag
     8701 gaatttaatc ttttgcaaca tgctttaaac atcgaagaag atattgataa tttgagatat
     8761 aatcatgttg tgatagcaac tgatgctgat gtggacggca tgcatattcg attgttaatt
     8821 atgacatttt tcttgcagtt ttttcctgat ttagtaaaaa aaggacatct atttgtatta
     8881 gaaacaccac tatttcgagt tcgtaataag caagaaacta tctattgtta ttccgagcaa
     8941 gaaaaacaaa aagcgattga taaactaaaa ggaaaacctg aaattactag atttaaagga
     9001 ttgggtgaga tttctccttc agaatttgaa gagttcatca atgaagatat tcgcttagaa
     9061 ccagttttgc ttaaacaaaa tcaaagtatt gagcagttgt tagaatattt tatgggaaaa
     9121 aatactccag aacgtcaaga gtttatcatc aataatctaa aagtagaaaa agacattgtt
     9181 gaagaagctt tgttgagcta atttatttaa acaaatggta cacaataaaa gaagcaatat
     9241 aagcaaataa gcccatgcct ataaattgat agattggata tttccatgtc tttgtttctc
     9301 gataaactac agccatggta ctcatacatt gtaaagcgaa tgcataaaat acaagtaagg
     9361 acagcgaagt ggcaactgta aatacttttt gcttagtttc tggatttatt tgtttgccta
     9421 atttctctct gatggtagat tctccagcat cttcaccaat actgtaaagc gtggacattg
     9481 ttccaataaa tacttctcta gcagcaaatg atgtgataat tgcaatgcca attttccaat
     9541 caaatccaat tggtttaatg actggttcaa taactcttcc agcaatacca gcataagaat
     9601 tttctaaagt tttagaagca atatattctg agctttcttg ttcagataaa ttcatttgag
     9661 cagcttcttg ggcaaattgt atttttttgt ttgccaaatt tggaccaaaa cttgcagcaa
     9721 accacaatac aatagatata actataatta cttttccagc accaaacaca aaagcttgta
     9781 ctttgttatt aacttccatc attacatttt tccaataagg tattttgtat tctggtaagt
     9841 ctaatgaaag gaatgaagtg tccgttgttt ttaaaatttt atgtagaata taggctgaac
     9901 ccaaagcagc gactacgccc aaaagataca tacccataaa aactaatgct tgtaaattta
     9961 aaaatccaaa aacaacagta ctcggtacaa caaatgcgat taataatgca taaattggaa
    10021 tacgggcaga acagcttata aacggtgtga ttaaaatggt aatcaatcgt tcttttgggt
    10081 tgctgattgt tctagtactc attattgcag gaattgcaca agctccgcct gaaatcaagg
    10141 caacaatact tcgtccattt agtccaaatc tttgcatcaa gctatcaaat aaataaactg
    10201 cacgagacat atagccaacc tcttccaaaa tggaaataat cagaaataat aaaaatattt
    10261 gtggaacaaa aatgacaatt ccgccaatac ctggaataat tccttcggtt aataatctag
    10321 taagtaaatt atcgggcata ttgtttgcaa tatttccagc cattctagta aactgtgcat
    10381 caataaaatc cataggaatg gtggaccaat tgaacatggc ttggaatact aataacatta
    10441 aagcaaaaaa gattaaaaca ccccaaaact tgtgggttaa aatattatct aatttgtcag
    10501 taaatgtatt agatgagttc aattgtcgct gtataatttt aggcaaatca ggcgtgaatg
    10561 cattgtatct atctaatatt tcatcaaatt gttctgtaat agaattaaat tgatgtttag
    10621 tttttagatt gtttaaatct aacctttgtt cagtacttat aaaatgaact tgtttgctgt
    10681 gttggaaaat taaccaactt ctataggtat cttcatttgg aattactgct cgaatttcat
    10741 ttagtatttc attttctatt ggatagaaaa ttttcaaatc agctggcgta tttaactgct
    10801 ctgctaatgt tttttttaat aaatcaaaat tatctccact tcttgcattg ataggaattg
    10861 cagggactcc aaaggttttt tcaaattgtt tgctatcaat tttaatatga ttttttttcg
    10921 ctacatctat catattgata gcaactacga ctttaaattt taaatctatt aattgggtaa
    10981 gtaataaaca atgtctttca atttgactgg catctaaaat atacagaata agttcaggaa
    11041 agtccttatt gtttttatcc aataaaatat ctaaaactat tttttcatca gcagaatttg
    11101 gataaagtga gtacgttcct ggtaaatcaa ttaaacttgc ttttgtattt ttatctaatt
    11161 gggaaaagcc tgttttttta tctacggtaa caccagtaaa gttgcccacc ttttgttgta
    11221 atcctgttag ttgattaaat acagaagatt ttccggcatt tggattgcca atcaatgcta
    11281 ttttagctac atttttttgc actgccactt tattcactta taataatact taatgcctct
    11341 tgctttctta atgcgatatt acaacaatta tttacacgta cataataggt cgagccaaag
    11401 agtgtttttc gcattaattc tactgaagca tttggcaaaa tacccatcga aattaactta
    11461 gaacctaata ttttatccgt gaaattctgg atagaactta tttctccttt tcttaagttc
    11521 gttatgtttt ttactgccac caatagttta gatttagtct taatagaata caaaagtacg
    11581 tgttaaaaag taaaatctat actgtatata tatttttacc taaaattaat tagagaataa
    11641 cattaattta cttttttata caccaatttg aaagaatatt tcgtttttat tctaatttta
    11701 tgccctatat tgtttacttt tatcagattg gtatcaaatt tgtattatta agataaatta
    11761 catttaaaat gaaaaaactg agtatgctta tattgttatt ttttgtttta ggttatacac
    11821 ataatctgaa agctcaaaat agtgattgga tagacattga gaatgctatt gcaaaaacag
    11881 atgcacagca tcaatttatt ttggtagata tttataccga ttggtgtggc tggtgtaaaa
    11941 aaatggatca gacagctttt gctgaccctg caactctagc aaatttgaaa aataactttg
    12001 tcttagtaaa gttaaatgct gaaggcgaaa atcctattaa ttttaatggc aagacatata
    12061 atcctgttcc aactggaaat agaaagacaa gtcaattagc aattgattta ggctctgttt
    12121 caggaagaat tggttatcca actttagttg ttttagatag taaaggtaat aaactacaag
    12181 ctttcccagg ttataaagat gcggacacta ttaatcaatt agtaaagtat tatgtttctg
    12241 g
//
LOCUS       DAOQCW010000074        12187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1942_length_12187_cov_5.473953, whole genome shotgun sequence.
ACCESSION   DAOQCW010000074 DAOQCW010000000
VERSION     DAOQCW010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12187
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1942_length_12187_cov_5.473953"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="PLS16_07700"
     CDS             <1..801
                     /locus_tag="PLS16_07700"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNJ61002.1"
                     /translation="DNDQIEQFHFNNFAEFFVYVIGDNINPLLDITFDGKHITDGEYI
                     SAKPEILFRLKDENKNLALNDTSAFLIYMYYPNNPTVPVLISNSNPDVVFIPADSSNL
                     EKINEAKLIFKPTLPDGTYEIRVQGIDRSNNDAGKYEYRIKFKVDSKPSISNVLNYPN
                     PFSTSTQFVFTITGSEVPNNILIQIFSQSGKVVKEITQAELGTLHIGTNITDYKWNAT
                     DNYGDRLANGVYFYRVLVKDPSGKNIEIKSSSADKYFKNGYGKMYIIK"
     gene            903..1931
                     /locus_tag="PLS16_07705"
     CDS             903..1931
                     /locus_tag="PLS16_07705"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HNJ61003.1"
                     /translation="MKHFYFLAMLIWVFTTQAQEAVANVEKKTITAEDKKRIEQDSIN
                     GWKFGGLGGVTFNQAGFVNWAAGGVNAISMNFNLRFYADLKKNKHLLQNWLAAEYGLQ
                     FSKQTYPRLNKNADRWEIFSKYGYQISKKTYIGGYIDLRSQFTNTYKYAADSSRTLTS
                     GFASPLIFEGAFGIDYVPNSYISLFFSPLASKVTFIGNNSIAQTEAYGNKWPSKTKSE
                     FGATLIAQYKQSFWKQNISINSIFRAYKNYLFASVDPYNTAIRETKSSYRKNIDVDWQ
                     TTLGFKVNKFITASVFTHLIWDHDIVIPNKDGLNAKPRLQFRDIIGIGLVYQTNYFKE
                     KEVKALQL"
     gene            2049..2846
                     /locus_tag="PLS16_07710"
     CDS             2049..2846
                     /locus_tag="PLS16_07710"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ61004.1"
                     /translation="MFQAQLFKDKIILVTGGGSGIGYTIAKQYLELGATVYIASRNKE
                     KIDKAIAELSKIGNVKGAIADIREPEHIEKLAQQIKEESGRLDILVNNAGGQFPSAAE
                     NISYNGFKAVINNNLIGTFYVTQIMAKTFFIPQQDGNIVSIVANMFRGFPGMVHTGAA
                     RAGVDNMTKTLAIEWTRYNIRVNAVAPGIIQSSGMDTYPPFILQGITDSIPNRRMGTT
                     EEVGWSVLFLSSPMASYINGETLYVDAGNRLWGDQWKMWSPSLTDKS"
     gene            complement(2856..6491)
                     /locus_tag="PLS16_07715"
     CDS             complement(2856..6491)
                     /locus_tag="PLS16_07715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNJ61005.1"
                     /translation="MQEQLIRKIIFAFGLILFLIGLNYFWIGKVNISKARNKIEKVIH
                     DKENAALNIFNNKKFLTNYNSPNYLHQLKSEIDEDGFYLFIFDRDELVYWSTNDVLIK
                     SPKVVSRNDKNFIKIDNGYYLAMHKYLPDMLHQAVILIPIYIQYSNTNQFLKSGFAFE
                     NNILKRIKITTKYINEKSIIKNLEGEYIFSIKENTSYSQISDFMFFILIALFIYLMYH
                     ISQLVKLLLQAGKQKTALLFVLIVVVLIELLFNHTELIIDFPDLELFSSKLYASNFLA
                     DNLGVLMIRVLLCIWCLYFLKYIRLPKSNFINNYVLVLVPIIHFYYVIYIIGNIINNS
                     TINFNFFSYTTIDFYSYISILLFGLLFFSLVQMLNFVFVHKKEKISIYIIIIAVCLFT
                     IYQSFQATNIYFECYLGLWLVSFILIFYIKNEIKTKYYFITSILILALLSMLLTTTLW
                     VQSAQKEFEIKKSKVHELFIERDVVEEFELSGKENNIQNDGFISKYLANPYLLNIDLS
                     DRVLRNLGGLNKNYIINIYAYDFDKNPIKGTSDKAAAYFDRILRFRNTAQISNNFYHI
                     PIKETGEKYIGKFEYKNDTIVNGYLYIELIPRVFKINSAYPELLSQNKTYYDEILKNY
                     AYAIYQNKKLIKSDGEFQYSTYFDFPINQSLEFTQEEQSGFNHLIYQNKDKIVVVSEP
                     EKSIWIAVSIFSFIVLNLLFLFILLDLFGFSTDLWVDTKIKDFFVNNTLQKQIQNYTI
                     ILLLLSLGILALITFVYFNYQYRNIYKSKIEQNTNIVVKNFKQLFNEYYPIYGEETYV
                     WVMQNRLQQRSDIFETDITAYNTNGDMIATSNPEIYENNLISKKINSDAYYALNTLRK
                     SKFINEESIGKLHFMSLYLAITTDGNTKLYLQFPHYNIDKYLRSEIILFLISLINIYV
                     FSLIVIAFAAVYLSKSATNSLSIISEHIKNVDLNKKNIPLEWNKTDEIGLLVKQYNNM
                     LKELEQSALLLAKSEREGAWSEMAKQVAHEIKNPLTPMKLSIQHLQRALMDNSPDIKT
                     LAEKISERLIEQIDVLSDIASAFSDFAKLPSTKNVQIDFMPILESVIDLFEETEHIHI
                     GFNSNIASPAIILADKNQLTRVFTNILKNAIQSIPKDKEGELEVTVQDKITSYLLSFK
                     DNGEGIPKDKHEKIFEPNFTTKTSGTGLGLAICRNIIDNINGKIWLESVEGNYTIFYI
                     EIPKIIV"
     gene            complement(6497..7261)
                     /gene="mazG"
                     /locus_tag="PLS16_07720"
     CDS             complement(6497..7261)
                     /gene="mazG"
                     /locus_tag="PLS16_07720"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNJ61006.1"
                     /translation="MDKNLKAFENLLNIMNDLREKCPWDKKQTIQSLRKLTIEETYEL
                     ADAILKEDWENIKEELGDLFLHLVFYAKIAQEQQRFDVAEVLDSVCQKLIFRHPHIYG
                     DVKVENEDDVKRNWELLKLQEGKKSVLGGVPDALPPINKAQRIQEKAKNVGFEWENKF
                     QVWAKVVEEIGELEEAEKSENIDKIEDEFGDVLFALINYARFLKIDAEEALERTNQKF
                     IQRFNFMENYANSNNIALSNMSLEEMEALWVKAKGK"
     gene            7373..8632
                     /locus_tag="PLS16_07725"
     CDS             7373..8632
                     /locus_tag="PLS16_07725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNJ61007.1"
                     /translation="MRTNVIRNIIILAFISLGGIIVTQVFWVKKNLDYKNEKFNDNAN
                     IATRNAINKIVIQTNMDDLYYKMEQDTTGEFNIFFSKPFPLKNFEASLYKEYKENNIK
                     TTITYIIRDFNHTQIKKGMIDLVNPKFKPEIFEFESIVQNIELNLDKNQSILLEPDIK
                     LLAFFSFVLLVVLGFFSYTTIVIMREKQLSKVKTDFVNNMTHEFKTPIATIGLASTVL
                     MKEDIYKMPEKLHHYATIINEENERLKKQVESVLQSSQIESKKIKLNVTAIDMHQLIA
                     EITKNFEPRIKEHEGNLTTKKEATDFMIKGDIVHIKNILFNLLDNGIKYANKIPQIIV
                     HTKNEKNYFVVSIHDNGKGISNDDKKKIFDKFYRVPSGNIHDVKGFGLGLFYVKDMME
                     QHKGFVSLKSTVDVGSTFSLWFPINKK"
     gene            8640..9338
                     /locus_tag="PLS16_07730"
     CDS             8640..9338
                     /locus_tag="PLS16_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ61008.1"
                     /translation="MKNNIKILLVEDDVNLGFIVKDNLEEKGYTVTHCTNGLDAEREI
                     YNQIFNIYVFDVMLPKKDGFTLATELREKGDTTPIIFLTAKEMQEDKIKGLSIGADDY
                     MTKPFNFEELVLRIDAILRRTLQSDFTSKIHTIGQFEFDVDNLTLVINQETRNLTKKE
                     AALLEQLILNVNLPVKRETLLNKIWKDNSYFAGRSMDVYITKLRKYLSDDPNVEIQNM
                     HGVGFKLVIKSTTK"
     gene            9405..10769
                     /gene="mgtE"
                     /locus_tag="PLS16_07735"
     CDS             9405..10769
                     /gene="mgtE"
                     /locus_tag="PLS16_07735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNJ61009.1"
                     /translation="MFEEAINLLESKSWFKLRTELTAIHPSNIAEFIESLEPEQQIIV
                     FRILPIKIAARTFEHIDLDTQQSLLKSLGQVKMADILNNMSPDDRTQLLEELPSHILR
                     KTLNLLSDEERKIASTLLGYPENSVGRMMTPYYISIKKEWTVAEVLEHIRKFGKKSET
                     VNMLYVVDEKGRLVDDVRIGHFLFSEPETKVEDLLDERYAYTTVTDDIEYAITLFKQY
                     DRQALPVLTHDGVLVGIITFDDIMDADEEENTEDMQKFGGMDALELPYVDTPLFEMIK
                     KRAGWLVLLFIGEMLTASAMAFFEDEIAKAVVLALFVPLIISSGGNSGSQAATLIIRS
                     LAISEITLKDWWFVMRREFLSGLLLGLILGIFGFARIVIWDNIFGIYGDHGIAIGITV
                     GITLIGIVLWGTLCGSMLPFILKKLGFDPATSSAPFVATLVDVTGLMIYFSIASVILQ
                     GKLL"
     gene            10735..>12187
                     /locus_tag="PLS16_07740"
     CDS             10735..>12187
                     /locus_tag="PLS16_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61010.1"
                     /translation="MPQLYYKENYYKTFSSVLFMATKPKTEISYAFCLHPENISQNTE
                     IFSFLLIEKKQVGTNYKTYHLEEDIFETYKFHGSVKKVFEEFTPEYIDFNINEIQKFL
                     KKQKVGSNIDSLLSKKIDRHFHELFQKVIDVQNENIKLYHKIINPKTGNFLTTKCIFS
                     NTQPILSFDIQLKKNKYLLETFITIDGQTHHLKDFNQNNFLLEKDNIYYLLDYEQYQT
                     LEYLERPTVKNYSTDKANFLKYVVHKLESQFTIEKHNYFDAEVLDIIPKNCLYLSEIN
                     SGAFLMLTPQWKYNDIIIDGEFKAIHEVKQNQEIYAVKRNLQVETDFISHIQQTHPNF
                     IKQRNGYFYLSFDEAKKKNWFFKTYHQFLDEDIEIIGMDMLQHFRYTPFPLESKLSNI
                     KVTGNIVEATFKVSFGKENVSLNELQKVVLNEQRSVLLKDNSIGVLTDEWLSEYASIL
                     KHSKINKDTISIPKWILVKEDESIKSNSLKFVIP"
BASE COUNT         4529 a         1751 c         1728 g         4179 t
ORIGIN      
        1 gacaatgacc aaatagaaca atttcacttt aataatttcg cagagttttt tgtatatgta
       61 attggcgata atataaatcc attattggat attacttttg atggaaaaca cattaccgat
      121 ggcgaatata tttctgcaaa acctgagatt cttttccgtc taaaagatga aaataaaaac
      181 cttgctttaa atgacacttc agcatttttg atttatatgt attatccaaa caatccaaca
      241 gtacctgttt taatctctaa ttcaaatcct gatgttgtat ttattcctgc tgattcttct
      301 aatttagaga aaatcaatga ggcaaaatta atattcaaac caacactacc agatggcacc
      361 tatgaaataa gagtacaagg tatagataga agtaataatg atgctggaaa gtatgaatat
      421 cgaattaaat ttaaagttga ttcgaaacct tcaatttcta atgttttaaa ttatcctaat
      481 ccattctcca cttctaccca atttgtattt accattactg gaagtgaagt tccaaacaat
      541 atccttattc aaatattcag tcaaagtgga aaagttgtaa aagaaattac tcaagctgaa
      601 ctaggaacgc tacatattgg tacaaatatt actgattata aatggaatgc cacagataat
      661 tatggagata gattagcaaa tggagtttac ttctaccgtg ttttagttaa agatcctagc
      721 ggtaaaaata tagaaattaa atcttcaagt gccgataagt actttaaaaa tggctatggc
      781 aaaatgtata taattaaata aacactaaac tacatataat ttattattta ataattgagt
      841 aataaaagat atgatagaat accttatttt tgcactttgt attaataatt aaaattaaaa
      901 aaatgaaaca cttttatttt ttagctatgc taatttgggt attcacaact caagctcaag
      961 aagcagttgc taacgtggaa aagaaaacaa tcactgctga agacaaaaaa agaatagaac
     1021 aagactctat caatggttgg aaatttggtg gcttaggtgg tgtaactttt aaccaagctg
     1081 gatttgtgaa ttgggctgct ggtggagtta atgctatctc tatgaatttt aaccttcgtt
     1141 tctatgctga tttaaaaaag aataagcatt tattacaaaa ttggttggct gcagaatatg
     1201 gcttacaatt ttctaaacaa acttatcctc gtttaaacaa aaatgctgac agatgggaaa
     1261 tcttcagtaa atatggttat caaatctcta aaaaaacata tattggtggt tatattgact
     1321 tacgttctca atttaccaat acttataaat atgctgcgga tagttcaaga acactaactt
     1381 ctggttttgc atctccactt atttttgaag gggcatttgg tattgattac gttccaaata
     1441 gttatatttc tttgttcttt tctcctttag cttctaaagt tacatttatt ggaaataact
     1501 ctattgcaca aactgaagct tatggtaata aatggccttc aaaaacaaaa agtgaatttg
     1561 gtgcaacttt aattgcacaa tacaaacaaa gtttttggaa acaaaacatc tctatcaatt
     1621 ctatattcag agcatataaa aactatttat ttgctagtgt agatccatat aatacggcta
     1681 ttagagaaac aaaatcaagt tatagaaaaa atattgatgt ggattggcaa actactttag
     1741 gatttaaagt aaataaattt attacagcat ctgttttcac acacttaatt tgggatcatg
     1801 atatcgttat tccaaataaa gatggtttaa atgcaaagcc acgtttacaa ttcagagata
     1861 ttataggtat tggtttagta tatcaaacta attattttaa ggaaaaagaa gttaaagcac
     1921 ttcaattata atataaaaag cattttattt ttttatgaat taaacatttg taaagaaaca
     1981 tacaaacttt ttaaaaatag ctcaattttt gagctatttt tttttatctt gactaaaaat
     2041 aaaatagaat gttccaagct caacttttta aagacaaaat tattttagta actggtggtg
     2101 gaagcggaat tggatatacc atagctaaac aatatttaga attaggtgca acagtatata
     2161 tcgcatcaag aaataaagaa aaaattgata aagccattgc tgaactcagt aagattggga
     2221 atgttaaagg tgcaattgcc gatattagag aacctgagca tatcgaaaaa ttagctcaac
     2281 agataaaaga agaatctggc agattagata ttttagttaa taatgctgga ggacaatttc
     2341 cttctgctgc tgaaaatatt tcatacaacg gattcaaagc tgttatcaat aacaatttga
     2401 taggtacatt ctatgttaca caaattatgg ctaaaacatt tttcattcct caacaagatg
     2461 gaaatattgt aagtattgtt gctaatatgt ttagaggatt tccaggtatg gtacatacag
     2521 gtgctgctcg tgctggtgtg gataatatga caaaaacttt agcgattgaa tggacaagat
     2581 ataatatacg tgtcaatgca gttgctcctg gaattattca atcttcaggt atggacacat
     2641 atcccccatt tattttacaa gggattactg attctattcc gaatagaaga atgggaacta
     2701 ctgaagaagt aggttggtct gtgttatttt tatcatcacc tatggcaagc tacattaatg
     2761 gtgaaacttt atatgttgat gctggaaata gattatgggg cgaccaatgg aaaatgtgga
     2821 gtccatcgct cactgacaag tcgtaatatg ctcaattata ctattatttt aggtatttca
     2881 atataaaaaa tggtatagtt tccttcaaca gattctagcc atatttttcc attgatatta
     2941 tctataatat ttctacaaat agctaaacct aaacctgttc cagatgtttt ggttgtaaaa
     3001 ttaggttcaa aaattttctc atgtttatct tttggaattc cctcgccatt atctttaaac
     3061 gataataaat aacttgtaat tttgtcttga actgttactt ccaattcgcc ttctttatct
     3121 tttggaatag actgtatagc atttttaaga atatttgtaa atacacgtgt taattgattt
     3181 ttatctgcca aaataattgc aggagatgca atatttgaat taaatcctat atgaatatgt
     3241 tctgtttcct caaataaatc aatgacagat tctaagatag gcataaaatc tatctgtaca
     3301 ttcttggtgg aaggtaactt tgcaaaatct gaaaatgccg atgcaatgtc tgataaaaca
     3361 tcaatttgtt caattaatct ctcagaaatt ttttctgcta atgttttaat atcaggcgaa
     3421 ttatccatta aggctctttg taaatgttga atacttaatt tcattggcgt aagtggattt
     3481 tttatttcat gagccacttg ttttgccatt tcactccacg ctccttcccg ctccgatttt
     3541 gccaacaata aagcactttg ttctaattcc ttcaacatat tattatattg ctttaccaac
     3601 aaaccaattt cgtcagtttt attccattcc aaaggaatat ttttcttatt caaatccaca
     3661 ttcttaatat gttcggagat tatactcaat gagtttgttg cacttttaga taagtaaact
     3721 gctgcaaatg caataacaat caatgaaaag acataaatat ttatcaatga aattaaaaat
     3781 agaattattt ctgagcgtag atatttatca atgttgtaat gtggaaattg taaatataat
     3841 tttgtgttac cgtctgttgt aatcgctaga tacaaactca taaaatgtaa ttttccaata
     3901 ctttcttcat taataaactt agactttcgc aaagtattta atgcataata ggcatctgaa
     3961 tttatctttt ttgagattaa attattttca taaatctcag gatttgaagt ggcaatcata
     4021 tcgccatttg tattgtaggc agtgatatct gtttcaaata tatcagaacg ttgttgcaat
     4081 ctattttgca taacccacac ataagtttct tcgccataaa ttgggtaata ttcattgaat
     4141 aattgtttga aatttttgac aacaatgtta gtattttgtt caattttact tttataaata
     4201 tttctatact gatagttgaa ataaacaaaa gtaattaatg ccaatatgcc cagagataac
     4261 aataatagta taatggtata gttttgaatt tgcttttgca aagtattatt gacaaagaaa
     4321 tctttgattt tagtatccac ccataaatct gtagaaaagc caaaaaggtc taataaaata
     4381 aataaaaata gcagatttaa aacaataaaa gaaaatatag aaacagctat ccatattgac
     4441 ttttcaggct cggaaacaac cactatttta tctttatttt gataaattaa atgattaaac
     4501 ccactttgtt cttcttgtgt aaattcaagg ctttggttta tcggaaaatc aaaataggtt
     4561 gaatattgaa attcgccatc gctcttaatt aattttttat tttggtaaat ggcgtaagca
     4621 taatttttta aaatctcatc atagtatgtt ttgttttgcg ataataattc tggataggca
     4681 ctattgattt taaacactcg aggtatcaac tcaatatata aatatccatt tactatggta
     4741 tcatttttat actcaaattt tccaatatat ttttctccag tttccttgat tggaatatga
     4801 taaaaattat tagaaatttg tgcagtattc ctaaatctca gtatcctatc aaaataggca
     4861 gctgccttat ctgacgtacc ttttatggga tttttatcaa aatcatacgc ataaatattg
     4921 atgatataat ttttattcaa tcctcctaaa tttctaagca ctctatctga caaatcaata
     4981 ttcaataaat aaggatttgc caagtattta gaaataaaac catcattttg gatattattt
     5041 tctttgccgc tcaattcaaa ttcttcaaca acatcacgct caataaataa ttcatgcact
     5101 ttgctctttt taatttcaaa ttctttttga gcagattgta cccataaggt tgttgtcaat
     5161 aacatagaca ataaagccaa aatcaaaata gaagtaatga aataatattt ggttttaatt
     5221 tcatttttta tataaaaaat caaaataaac gagactaacc aaagtcctaa gtaacattca
     5281 aaataaatat ttgtagcctg aaagctttga taaatcgtaa ataaacaaac ggcaataatt
     5341 atgatataga tactgatttt ttctttttta tgaacaaaaa caaaattcag catttgtacc
     5401 agactaaaaa acaaaagtcc aaaaagtaga atagagatat aagaataaaa atcaattgtt
     5461 gtataactaa agaaattaaa atttatagta gaattgttaa tgatattccc aataatatat
     5521 atcacgtaat aaaaatgaat tattggtact aaaaccaaaa cataattatt aatgaaatta
     5581 gattttggca atcgaatata tttcagaaaa tacaagcacc aaatacataa caaaactcta
     5641 atcattaaaa cacctaaatt atcggctaga aaatttgatg catacaattt agaggagaac
     5701 aattctaaat cgggaaaatc tataattaat tcagtatgat tgaataataa ttcgattaag
     5761 acaacaacta tcaaaacaaa caaaagagca gttttttgtt tcccagcttg caataataat
     5821 ttgaccaatt gagaaatatg gtacattaaa tatataaaca aagcaatcag tatgaaaaac
     5881 ataaaatcag aaatctgtga gtagcttgta ttttctttta tggagaaaat atattcacct
     5941 tctaaatttt ttataatgct cttttcattg atatacttag tcgtaatttt aatacgttta
     6001 aggatattat tttcaaaagc aaatcctgat tttaaaaatt gattggtatt actatattga
     6061 atataaatgg gtattaaaat aactgcttga tgcaacatat ctggtagata tttatgcatt
     6121 gccaaataat agccattgtc aattttaata aagtttttat cattcctcga aacaactttt
     6181 ggacttttaa ttaaaacatc atttgtggac caataaacta attcatctct atcaaaaatg
     6241 aacaaataaa acccatcttc atctatttca ctttttaatt gatgtaagta attgggacta
     6301 ttataatttg taagaaattt tttattgttg aaaatattta aagcagcatt ttctttatcg
     6361 tgaatgacct tttcaatttt atttcgagct ttggaaatat ttactttacc tatccaaaaa
     6421 taattcaagc cgattaaaaa aagtattaat ccaaaagcaa aaattatttt acgtataagt
     6481 tgttcttgca tgataactat ttgccttttg ctttaaccca aagtgcttcc atttcttcca
     6541 aactcatatt agacaaagct atattattgg aatttgcata attttccata aagttaaatc
     6601 tttgaataaa cttttggtta gttctttcta atgcttcttc agcatctatc ttcaaaaatc
     6661 gtgcataatt tatcaatgca aatagcacat cgccaaattc atcttcaatt ttatctatat
     6721 tttcagattt ctcagcttct tctagttctc cgatttcttc tacaacttta gcccaaacct
     6781 gaaatttatt ttcccattca aatccaacat tcttggcttt ttcttgaatt ctttgagctt
     6841 tattgattgg aggcaaagca tcaggcacac cacctaacac actttttttg ccttcttgta
     6901 atttcaataa ctcccaattc cttttaacat catcttcatt ttctacttta acatcgccat
     6961 aaatgtgtgg atgcctgaag attaatttct gacacacact atctaaaacc tcagcaacat
     7021 caaaacgttg ttgctcttgt gcaatcttcg cataaaaaac caaatgcaaa aataaatctc
     7081 ctaattcctc ttttatattc tcccaatctt ctttcaaaat tgcatctgct aattcatagg
     7141 tttcttcaat agtcaattta cgaagtgatt gaatggtctg ttttttatcc caaggacatt
     7201 tttctcttaa atcattcatg atattcaata aattctcaaa agccttcaga tttttgtcca
     7261 tttcttaaat tttatacaaa actaataaaa ctaaaccatc atctatatat ttataaaaat
     7321 acatttcgta atattatcgt ttaattagta tatttaattt aaaaagggca gaatgcgtac
     7381 taacgtcatc cgaaatatta taattcttgc atttatttca ttaggtggca ttatcgttac
     7441 acaggtattt tgggtaaaaa agaatttaga ttataaaaac gaaaaattta acgacaacgc
     7501 caatattgca accagaaatg ccattaacaa aatcgttata caaacaaata tggacgattt
     7561 atattacaaa atggaacagg atacaacagg tgaatttaat atctttttta gtaagccatt
     7621 tcctttaaaa aattttgaag catcattata taaagagtac aaagagaata atataaagac
     7681 tacaataacc tatattattc gagatttcaa tcatacacag attaaaaaag gtatgattga
     7741 tttagtaaat cctaaattca aacctgaaat atttgagttt gaatctatcg tccaaaacat
     7801 tgaattgaat ttagataaaa atcaaagtat tttattagaa ccagatatca aactattagc
     7861 attcttttct tttgttttat tagtggtatt aggatttttc agttatacaa cgattgttat
     7921 tatgcgagaa aaacaattga gtaaagttaa aactgatttt gtcaataata tgacacacga
     7981 atttaaaaca ccaattgcta caatcggttt ggctagtact gttttaatga aggaagatat
     8041 atataaaatg cctgagaaat tacatcacta tgcaacaatt atcaacgagg aaaatgaacg
     8101 tttgaaaaag caagtagagt cggttttaca atcttctcaa attgaatcta aaaagataaa
     8161 attaaatgta acagctattg atatgcatca attgattgct gaaattacta aaaattttga
     8221 acctcgaatt aaagaacatg aaggaaattt aactactaaa aaagaagcta cagactttat
     8281 gataaaaggc gatatcgttc atatcaaaaa tattttattt aacttgcttg ataatgggat
     8341 aaaatatgca aacaaaatac ctcaaataat tgttcatact aaaaatgaaa aaaattactt
     8401 tgtcgtaagt attcacgata atggaaaagg aattagcaat gatgataaaa agaaaatatt
     8461 tgataaattt tatagagtac cgagcggtaa tattcatgat gtgaagggat ttggcttagg
     8521 tttattttat gtaaaagata tgatggaaca gcataaaggt tttgtatctt taaaaagtac
     8581 agttgatgtt ggttctacat tttcattatg gtttccaata aataaaaaat aaaaggctta
     8641 tgaaaaataa tattaaaata ctattagtcg aagacgatgt aaacttagga tttattgtca
     8701 aagataatct tgaagaaaag ggctatactg taacacattg cacaaatgga ctcgatgcgg
     8761 aacgtgagat ttataatcaa atattcaata tctatgtttt tgatgtaatg ctacccaaga
     8821 aagatggttt taccttagca acagaattaa gagaaaaagg agatactacg ccaattatat
     8881 ttttaacagc taaagaaatg caagaagata aaattaaagg attgagtatt ggtgcagatg
     8941 attatatgac aaaacctttt aattttgaag aattagtgct tagaattgat gcaattttaa
     9001 gaagaacact tcaaagtgat tttacttcaa aaatacatac gattggtcaa tttgaatttg
     9061 atgttgataa tcttacatta gttattaatc aagaaacaag aaatttgact aagaaagaag
     9121 ctgctttatt agaacagctg attttaaatg tcaatttgcc tgtaaaaaga gaaactttac
     9181 tcaacaaaat atggaaagat aatagctatt ttgcaggaag aagtatggat gtttacataa
     9241 caaaactacg aaaatactta tctgatgacc caaatgtgga gatacagaat atgcacggcg
     9301 ttggctttaa attagttatt aaatctacta ctaaataaaa tcaatttcaa aataagttcc
     9361 aaataatttt tggataatat tccttatttt tgttgctcta caatatgttt gaagaagcaa
     9421 taaatttatt agaatctaaa agttggttta agctcagaac tgagctaact gctatacatc
     9481 caagtaatat tgctgaattc attgaaagtt tagaaccaga gcaacaaatc attgttttta
     9541 gaatacttcc gataaaaatt gcagctcgta cctttgaaca tatagattta gatacacaac
     9601 aatctttact aaaatcatta gggcaagtaa aaatggctga catcttaaat aatatgtcgc
     9661 ccgatgacag aacccagctc ttagaagaat tgccttcaca tattcttaga aaaacactca
     9721 atttactttc cgatgaagaa cggaaaattg cctccaccct attaggttat cctgaaaaca
     9781 gtgtgggcag aatgatgaca ccctactaca ttagtattaa aaaagaatgg acggttgctg
     9841 aagtattaga acatatcaga aaattcggaa aaaagagtga aaccgtcaat atgctatatg
     9901 ttgttgatga aaaaggtaga ttggttgatg atgttagaat tgggcatttt cttttctcag
     9961 aaccagaaac aaaagttgaa gatttattag acgaaagata tgcctataca actgtaacag
    10021 atgatataga atatgcaatt actttattca aacaatatga ccgacaagca ttgccagttc
    10081 taacacacga tggtgtttta gttggaatta tcactttcga tgatatcatg gatgctgatg
    10141 aggaagagaa cactgaagat atgcaaaaat ttggtggtat ggatgcatta gaattacctt
    10201 atgttgatac gccattattt gaaatgataa aaaaaagagc aggttggtta gttttgctat
    10261 ttattggaga aatgctaaca gcatcagcaa tggcattttt tgaagatgaa atagccaaag
    10321 ccgtagtctt agcactattc gttccactaa tcatctcgag tggtggaaac tctggttctc
    10381 aagctgcaac attaatcata cgttcattag caatttctga aataacccta aaagattggt
    10441 ggtttgtaat gagaagagag tttttatcgg gactattgct aggtttaatt ttaggaattt
    10501 ttggcttcgc cagaattgta atttgggaca atatctttgg catctatggt gatcatggca
    10561 ttgctattgg cattacagta ggaattacat taattggcat tgtgctttgg ggaacacttt
    10621 gcggctctat gctacctttt attcttaaaa aattaggttt tgacccagca acctcttcag
    10681 ctccatttgt tgcaacccta gttgacgtaa ctggattaat gatttatttc tctattgcct
    10741 cagttatatt acaaggaaaa ttattataaa actttctctt ctgtattatt tatggctact
    10801 aaacctaaaa cagaaatatc ctacgcattt tgtttacatc cagaaaacat ttctcaaaac
    10861 actgaaattt ttagctttct attaattgaa aaaaaacagg taggtacaaa ttataagaca
    10921 taccatttag aagaagatat ttttgaaact tataagtttc atggtagcgt caaaaaagtt
    10981 tttgaagaat ttacgccaga atatattgat ttcaatataa atgaaattca aaaattccta
    11041 aaaaaacaaa aagttggaag taatattgat agtttattat ccaaaaaaat agatagacat
    11101 ttccacgaat tatttcaaaa agttatagat gttcaaaacg aaaatataaa actctatcat
    11161 aaaataatca atcctaaaac tggaaatttt ctaactacaa aatgtatatt ttcaaataca
    11221 cagccaattt tatcttttga tattcaactt aaaaaaaaca aatatttgct tgaaactttc
    11281 ataacaatag atgggcaaac acatcactta aaagatttta atcaaaataa ttttctatta
    11341 gaaaaagaca atatttatta tcttttagat tatgagcaat atcaaacttt ggaatatctt
    11401 gagcgtccaa ctgtaaaaaa ttatagcacc gacaaagcaa attttcttaa atatgtagtt
    11461 cacaaattgg aaagtcaatt tacaattgaa aaacacaatt attttgatgc tgaagtactt
    11521 gatattattc ctaaaaattg tctttattta agtgaaatta atagtggtgc atttttaatg
    11581 cttacacctc aatggaaata caatgacatc ataattgatg gggaatttaa agcaatacat
    11641 gaagtaaaac aaaatcaaga aatttatgcc gtaaaaagaa atctgcaagt agaaactgat
    11701 tttataagtc atattcaaca aacacatccc aatttcatca aacaacgaaa tggctatttt
    11761 tacctatcct ttgatgaagc aaaaaagaaa aattggtttt ttaaaaccta tcaccaattt
    11821 ttagatgaag atattgaaat tattggaatg gatatgttgc aacatttcag atatactcca
    11881 tttccattag aaagtaaatt aagcaatatc aaagtaactg gaaatattgt ggaagccact
    11941 tttaaagtat cttttggaaa agaaaatgtt agtctaaatg aattacagaa agtcgtcttg
    12001 aatgaacaac gttctgtttt actaaaagac aattcaattg gagttttaac agacgaatgg
    12061 ttgtcagaat atgcttccat tttaaagcat tctaaaatca ataaagacac tatttcaatt
    12121 ccaaaatgga tactagttaa agaagatgaa tctataaaat ccaatagttt aaaatttgtt
    12181 attcctc
//
LOCUS       DAOQCW010000075        12163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_1950_length_12163_cov_5.725223, whole genome shotgun sequence.
ACCESSION   DAOQCW010000075 DAOQCW010000000
VERSION     DAOQCW010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12163
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1950_length_12163_cov_5.725223"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="PLS16_07745"
     CDS             complement(<1..1193)
                     /locus_tag="PLS16_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNJ61011.1"
                     /translation="MKLNFKQRLFFYFTVIFTLSIIGVTIFERNKEKVQKTETLEGIL
                     DVYTTTISSFLSKNPTDNFSNLDSLVSLFPSELRVSIIDKEGIVNYDNTIKNINILSN
                     HAQRPEILALKTSNKGTDIRNSASKNQAYLYYAKDNGINFIRVALPYNINTKNLLKPD
                     NSFVYFILIIYLSVLFLINSIINKFGKSVTQLRDFAIHAKVIPSENNFSDNELDEIGK
                     KLTENYLLLEKSKNEVNAEKEKLLQHIHNSKEGICFFTPQKEVEFYNGHFIQYLHLIT
                     DSEEGNPNVIFTDKAFEQLHLFLKNSTANFLDDKISKQGKIFHLRLVFFENKGFEIIL
                     NDITLQEKTRVLKQEMTGNIAHELRTPVTGIRGYLETILTQKLEIDKQLYFTEKAFLQ
                     TKVLS"
     gene            complement(1190..1882)
                     /locus_tag="PLS16_07750"
     CDS             complement(1190..1882)
                     /locus_tag="PLS16_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ61012.1"
                     /translation="MANKILIVDDDVSICEILEFNLKSEGYIVEIAHSAEEALEQLTP
                     DFNLILLDIMMGGMSGYKMAEKVKASKNRTPIIFLTAKDTENDMLTGFSVGADDYISK
                     PFSIKEVLARIKAVLARINQGSKSSLTNTQILELNDFIINFDTKQLSIKGNKISLTKT
                     EFDILLKLIENPNKIFSRQEIIDSIWGDEVYITERTVDVHITRLRKKLGDYASAITNR
                     SGFGYRFDSNNL"
     gene            complement(1929..2606)
                     /gene="phoU"
                     /locus_tag="PLS16_07755"
     CDS             complement(1929..2606)
                     /gene="phoU"
                     /locus_tag="PLS16_07755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNJ61013.1"
                     /translation="MRHTELEIQELKTQVKQMWNLVVAQLSKTKKALLENDTALALEV
                     ISQEKRVNAYELKIESDCENYIALYSPVAVDLRLALTLIKISNTLERIGDFAEGIARH
                     TISQECNVIDTKIMEDLGVEKMMDTVLEMLLDGYIAFETENTKLTEKILSLDIQVDEL
                     YKNGFFILTEYLNTQEKNAMCALKLIILLRKIERIGDHCNNIIEDIVFYVDAEVLKHH
                     KKITINQ"
     gene            complement(2625..3374)
                     /gene="pstB"
                     /locus_tag="PLS16_07760"
     CDS             complement(2625..3374)
                     /gene="pstB"
                     /locus_tag="PLS16_07760"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNJ61014.1"
                     /translation="MMIEAKNINFYYGNFHALKGINIAIPANTVTALIGPSGCGKSTF
                     LRLFNRMNDTIQNTKLTGECLVNGQNIYDKTVQVDDLRKNVGMVFQKPNPFPKSIFEN
                     VAYGLKVNGISDKKYIAERVEQSLIAAALWNEVKDKLHQSAFALSGGQQQRLCIARAL
                     AISPSILLMDEPASALDPISTSKIEELIHELKEKYTIVIVTHNMQQAARVSNKTGFFM
                     YGELIEYSDTKKMFLNPENIQTQNYITGRFG"
     gene            complement(3386..4243)
                     /gene="pstA"
                     /locus_tag="PLS16_07765"
     CDS             complement(3386..4243)
                     /gene="pstA"
                     /locus_tag="PLS16_07765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNJ61015.1"
                     /translation="MIRSKNIQQKIAFHIFRLLSFGILGILVWILGFIIYNGIQVISW
                     EFITSYPKEGMTEGGIFPAIIGTIYLVIGSMIFAVPLGIMSAIYISEYASNQKWVRFI
                     RIMTNNLASIPSIVFGLFGMALFVHTFGFGDSILAGSLTLGILIIPIVIRTTEEALKA
                     VPESYRLGSFALGATKLQTIKNVVFPIAFPNIMTGLILSVGRVSGETAPILFTVAAYF
                     LPKLPQSIFDQAMALPYHLYVIATSGTDIEASKPIAYGTAFVLISIVLTINLLAGWIR
                     RYFEKQNSI"
     gene            complement(4240..5436)
                     /gene="pstC"
                     /locus_tag="PLS16_07770"
     CDS             complement(4240..5436)
                     /gene="pstC"
                     /locus_tag="PLS16_07770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNJ61016.1"
                     /translation="MKIIERLLEGVFKMSSSITSIVILLIIIFLFKEGFGLFRSPVVE
                     NGYGLYINSKNPISDLNPTQIKQIFDGEIDNWKAIGGKEESIYLFRFDEIFSIYTDEE
                     LGENYQFLPQKLNNIISENSNIIAFFPKKYMQEVSSQTKEIANKNIEIADFILGKEWL
                     PTTTPSPLFGIVPLVLGTLLVSFLAILLALPLGLGVAIYLSEIASNKVRGIIRPAIEL
                     LAGIPSVVYGFFGLVIIVPIVQNLGNISAGETAFTGSLILAIMALPTIISISEDALRN
                     TPLALRESSYALGATQWQTIYKVVIPFASSGISAAIILGIGRAIGETMAVLMVTGNGA
                     VIPHSIFESVRTIPATIAAELGEAPMGGTHYQALFLLGCVLFLFTLILSITSEIISKK
                     QTSKTA"
     gene            complement(5472..6287)
                     /locus_tag="PLS16_07775"
     CDS             complement(5472..6287)
                     /locus_tag="PLS16_07775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNJ61017.1"
                     /translation="MKKTIILTISLFCLVLNIKAQKIKGSDTVLPLSQEAAENYKKKN
                     PTSTISVTGGGSGVGISALISGTTEIAQASRKIKFDEKLKIQESGNSLKEIIIAYDAL
                     SVIVNPSNKVSKLTREQLEGIFTGKITNWKEVGGDDLKIVPYSRETSSGTYEFFKESV
                     LKNKNYKSGIMSLPATGAIIQSISQTKGAIGYVGFAYLNTEVKTINISYDKGKTYVVP
                     NITNVKNKTYPVVRPLYYYYLTKNSNKVKGFVDYILSPEGQKIVSNVGYITLK"
     gene            complement(6394..7650)
                     /locus_tag="PLS16_07780"
     CDS             complement(6394..7650)
                     /locus_tag="PLS16_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61018.1"
                     /translation="MKKKIMMMSCLLLSIASFSQTEPVDSNALEKRITEIEETLKENK
                     NFKVSGYIQTQFQFGEQFSALKVGASNDNKNKTINRVGIRRGRIKFTYEKGIVAGVFQ
                     LDITEKGIGIKDAYLNIKDPWLKSMALKAGVFDRPFGSEISYSSSQREAPERSTIFQT
                     LFPEERDLGLMLTLQAPKTSIWNILKLEAGLFAGNGIKMETDNRKDFIGHLSFNHTIK
                     DILKIGGGISYYNGGVYQGSKNIYTMKGNSFVLNNDSTNIGKYAKREYIGFDMQINAI
                     TKVGTTKLMAEYLMGTQPGTNKDSKSPNASTLPTSDTYIRKFKGGYIMLTQDFGKVPL
                     TGVFKYDWYNPNKNVSKDNIGLNQTNKGDISYNTFGLGMVWKATSSIRVTAFYDIVRN
                     ETTNNITDYNQNRKDNVFTLRFQYKF"
     gene            complement(7806..9143)
                     /locus_tag="PLS16_07785"
     CDS             complement(7806..9143)
                     /locus_tag="PLS16_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61019.1"
                     /translation="MKKQTLLIGIFSVGNIVLAKEVSKSRIQETANNIATELQIPAAQ
                     RVNFTNEYTSKSIAIQDIVNKNVTPQEKVNQIKMVLNQSSANLKQVTPVVQQNRYSNY
                     FNKQVQMTAKVETLKSPASKNDNVNTKPNTPTSNNTNSAYTKNADYNNLPQEHQAVMD
                     NIGKQLVLDNNQFGKMSSDYLNFVTTTESIAKTNGKNLLKAKTEFDQLTASTLNSTKG
                     YLSTEQYNAFQTLLKTGKLGKDTNPRVNVANTNTTNNTTIVNNNKNLSDIKSAETLTQ
                     LKSSLNLSPTQYANALKVANNYDAEIAKVSAMYPNNIKQQKSELDKKTPMFVNQFKAA
                     LNPAQANSFFGVIIAQVNILTGENLTTEQQQLISAMRNNYKMNDVQIVQSMFALAEAK
                     VKADANKMVNKANPQVFNQENQKILSETDNKIKNILTPEQYNKVKADIEKALK"
     gene            9512..10143
                     /locus_tag="PLS16_07790"
                     /pseudo
     CDS             9512..10143
                     /locus_tag="PLS16_07790"
                     /inference="COORDINATES: protein motif:HMM:NF016013.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
     gene            complement(10300..11001)
                     /locus_tag="PLS16_07795"
     CDS             complement(10300..11001)
                     /locus_tag="PLS16_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HNJ61020.1"
                     /translation="MASNNIYHLFKQDKKQIATLIDPDKADVISLKKLVEATASADFY
                     FIGGSLLNEDNIEKIILALKQLTSKPVIIFPGNGMQICDKADAILLLSLISGRNPELL
                     IGQHVQFASILKNSGLEIISTGYILIDGGKPTSVSYISNTTPIPNNKPEIAAATAIAG
                     TQLGLKCIYLEAGSGAEIVISTDCINMVKAQIDVPLIVGGGIKSKAQIQAAFNAGADI
                     VVIGNALEKDLSLFD"
     gene            11068..11562
                     /locus_tag="PLS16_07800"
     CDS             11068..11562
                     /locus_tag="PLS16_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61021.1"
                     /translation="MTNKHLYTFLFDVFSLIFVSILSFLILFNMKEWMTQKYYIPLFL
                     YFTIAAFYFRCIIFPKSSFLFSFWPFLVVIFLGNFYITFKLFHNYYLFLDVIDDYSIH
                     FTKDFVTPLIKGLTAGQLLSIKSMATFGFVFYMVIIVLTQFRIIWLLLRKARAKFASK
                     DSNY"
     gene            complement(11559..>12163)
                     /locus_tag="PLS16_07805"
     CDS             complement(11559..>12163)
                     /locus_tag="PLS16_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61022.1"
                     /translation="YKKLLHVETADIQVEVLGNELLAILLEEDYKQHYQPKYNKIQKY
                     YEFSFGLFASKDENGFEKLVVKVLDEAEEPILKFTSKLKAEKMLHQIVNGSRLGPIFK
                     RIDTVLNYNERMESILAKYQYPFSSFYIIIAGRMGSEKCAIKIKSNALVGYTFYETTY
                     IQHIQELDNLLKPIKEFNNTKKAIINYIRKYEKYIQIIPV"
BASE COUNT         3864 a         1926 c         1691 g         4682 t
ORIGIN      
        1 tctgacaata cttttgtttg taagaatgct ttttcagtaa aataaagttg cttatcaatc
       61 tctaattttt gagtaagaat agtttccaaa taacctctaa ttcctgttac tggcgttctg
      121 agttcatgag caatattgcc cgtcatttct tgctttagaa ctcttgtttt ctcttggagt
      181 gtgatatcgt tcaagataat ttcaaatcct ttgttttcaa aaaatactaa acgaagatgg
      241 aatattttac cttgtttaga aattttatca tctaagaaat ttgcagttga attttttaag
      301 aaaaggtgta gttgctcaaa tgctttatct gtgaaaatca cattgggatt accttcttca
      361 gaatctgtta taagatgtag atattgaata aaatgtccat tgtaaaattc tacttccttt
      421 tgaggcgtaa aaaagcaaat accttctttg gaattatgaa tatgttgcaa taacttttcc
      481 ttttctgcat ttacctcatt ttttgatttt tctaataaca gataattttc agtaagtttt
      541 ttaccaattt catctaattc attatctgaa aaattatttt ctgatggaat aaccttcgca
      601 tggatagcaa aatctcgcaa ttgggttact gactttccaa atttattaat aatactattg
      661 attaaaaata gtacagataa atagatgatt agaatgaaat aaacaaagga attatcaggt
      721 tttagaaggt ttttagtatt tatattataa ggtaaggcta cacgaataaa atttattcca
      781 ttgtctttgg cataatatag ataagcttga ttcttagatg cagaattacg aatatcagta
      841 cctttattac ttgtctttaa tgcaagaatt tcaggtcgct gagcgtggtt acttaagatg
      901 ttgatatttt taattgtatt gtcataattt acaattcctt ctttatcaat tatgcttact
      961 cggagttctg aaggaaataa ggaaactaaa ctatctaaat tagaaaagtt atctgttgga
     1021 tttttagata aaaacgaact aatggttgtg gtgtaaacat caagaattcc ttctaaggtt
     1081 tctgtttttt gtactttttc tttatttcgt tcaaaaatag ttactccaat tattgagagt
     1141 gtaaaaatta ccgtaaagta aaaaaataag cgttgtttaa aatttagttt cataaattat
     1201 ttgaatcaaa tcgataacca aatccagaac gattggtaat tgccgatgca taatctccca
     1261 atttttttct taatcgtgta atgtgtacat caacagtacg ttcagtaata tatacctcat
     1321 cgccccaaat actatcgata atttcttgac gagaaaagat tttattaggg ttttctatta
     1381 gcttcagtag aatatcaaac tctgtttttg ttagacttat tttattgcct ttaatgctta
     1441 attgtttagt atcaaaattt ataataaagt catttaattc aagaatctgg gtattggtta
     1501 aacttgattt agagccttgg tttattcgag ctaatacggc tttaattcga gccaaaactt
     1561 cttttataga aaatggttta gatatataat catcagcacc tactgaaaat ccagtaagca
     1621 tatcattttc agtatctttt gcggttaaaa aaataattgg agtccgattt ttagaggcct
     1681 ttactttttc tgccatctta taaccggaca tcccacccat cattatatct aatagaatta
     1741 gattaaaatc tggggttaat tgctcaagtg cttcttctgc tgaatgagca atttcaacaa
     1801 tatagccctc actcttcaaa ttaaattcta aaatctcaca aatgcttaca tcatcatcaa
     1861 ctatcaatat tttatttgcc atttatagaa ttttcttgta aaacaaattt aagtataaat
     1921 gtctaatttt attgattaat cgttattttc ttatgatgtt ttaaaacttc tgcatctaca
     1981 taaaatacga tatcttctat gatattatta caatggtctc caattcgttc tatctttcgg
     2041 agtaagatga ttaatttcaa agcacacata gcattttttt cttgagtatt tagatattcc
     2101 gttaaaataa aaaatccatt tttgtacaat tcatctactt gtatatcaag gctcaatatt
     2161 ttttcagtta gttttgtatt ctcagtttca aacgcaatat aaccgtcaag cagcatttct
     2221 aaaacggtat ccatcatctt ttcgacacct aaatcttcca ttattttagt gtcaatgaca
     2281 ttacattctt gagaaattgt atgtcgtgca ataccttcag caaaatctcc aattcgttcc
     2341 aaagtattac taatcttaat taatgtaaga gctaatctca aatcaacagc aactggacta
     2401 tataatgcaa tataattttc acaatcactt tcgattttga gttcataagc atttactctt
     2461 ttttcctgac taataacttc aagtgctaat gctgtatcgt tttctaataa agctttttta
     2521 gttttactta actgagctac aactaagttc cacatttgtt ttacttgagt ttttagctcc
     2581 tgaatttcta actctgtatg tctcatattt acttttttta ggatttaacc aaatcttcca
     2641 gtgatataat tttgtgtttg aatattctct ggatttaaaa acattttctt agtgtcgctg
     2701 tactctatca attctccata cataaaaaaa cctgttttat tacttactct tgcagcttgt
     2761 tgcatattat gtgtaacaat tacaatggta tatttttctt tcaattcatg gataagttct
     2821 tcaattttag aagtggatat tgggtctaag gctgatgcag gttcgtccat tagtaatata
     2881 gatggcgaaa tggctaatgc tctagctata caaagccttt gttgctgacc accagacaat
     2941 gcaaatgctg actgatgaag tttatctttt acttcgttcc ataaagccgc tgcaataagt
     3001 gattgttcca cacgttctgc aatatacttt ttatccgata tgccatttac ttttaagcca
     3061 taagcaacat tttcaaagat agatttagga aatggattag gtttttgaaa taccattcca
     3121 acatttttcc ttaaatcatc aacttgtaca gttttatcat aaatattttg tccattaact
     3181 aaacattctc cagttaattt agtgttttga atggtatcat tcattcgatt aaataatcgt
     3241 aagaacgtag atttaccaca gccagaagga ccaattagtg ccgttactgt atttgcaggt
     3301 atggcaatat taataccttt caatgcatga aaattaccat agtaaaagtt tatatttttt
     3361 gcttcaatca tcatttttat gaatattata ttgagttttg tttttcaaaa tatcttctaa
     3421 tccaaccagc taataaattt attgttagaa cgatagaaat taatacaaat gcagttccgt
     3481 aagcaatagg ttttgaggct tctatatctg tgccgctagt tgcaataaca tacaaatgat
     3541 aaggaagtgc cattgcttgg tcaaaaatac tttgaggaag tttaggtaaa aaatatgccg
     3601 ccaccgtaaa taatattgga gctgtttctc cagaaactct acctacggag agaattaatc
     3661 cagtcattat atttggaaat gcaattggaa atacaacatt tttaatggtt tgaagtttag
     3721 ttgcacctaa tgcaaaacta cctaaacgat aagattctgg aacagctttt aaagcctctt
     3781 ctgttgtacg gattactata ggaataatta gaattccaag agttaaggaa cccgcaagaa
     3841 ttgaatctcc aaatccaaag gtatgcacaa ataaagccat tccaaaaagt ccaaatacaa
     3901 tagatggaat gctggctaaa ttatttgtca ttatacgaat gaatcttacc cacttttgat
     3961 tacttgcata ttcactgata taaatggcag acataattcc taagggaact gcaaatatca
     4021 tacttccgat tactaaataa attgttccaa tgattgctgg aaaaattcct ccctcggtca
     4081 ttccttcttt tggataggaa gtgataaatt cccaactaat aacttggatt ccgttatata
     4141 taataaaacc tagtatccaa accaagattc ctagaatacc aaaacttagt aatctgaaaa
     4201 tatgaaaagc tattttttgt tgaatgtttt ttgaacgtat catgctgttt tagaagtttg
     4261 ttttttagaa ataatctctg aagtgatact gagtattagt gtaaataaaa acaatacaca
     4321 ccctaataaa aacagtgctt gataatgtgt tccacccatc ggagcttcac ctaattctgc
     4381 tgcaattgtt gcaggaattg ttcggacaga ttcaaaaata gaatgtggga taacagcacc
     4441 attaccagta accattaaca cagccattgt ttcgcctatt gctcttccaa ttcctaaaat
     4501 aatagctgca gatatgccag aacttgcgaa aggaataacc actttataga tggtttgcca
     4561 ttgggtagca cctaaggcat aactagattc tcgtaaagcc aatggcgtat ttcgtaaagc
     4621 atcttcagag atggaaatta tcgttggcaa tgccataatt gctaatatca gactaccagt
     4681 aaatgccgtt tctcctgcac ttatattgcc caaattttga acaataggca ctataattac
     4741 taatccaaaa aacccataaa caaccgatgg aatacctgct aataattcga tagcaggtct
     4801 tattattcct cttactttat tactagcaat ttctgataag taaatagcta cacctaatcc
     4861 taaaggaagt gctaatagaa tggctaaaaa actaactaat aatgttccaa gaactaatgg
     4921 cacaattcca aataaaggag aaggggttgt tgttgggagc cattcttttc ctaaaataaa
     4981 atctgctatt tctatatttt tattcgctat ttcctttgtt tgactagaaa cttcttgcat
     5041 atatttttta ggaaaaaatg caataatgtt tgagttttca gaaattatat tatttagctt
     5101 ttgaggtaaa aattggtaat tttctccaag ttcttcatct gtatatattg aaaaaatttc
     5161 atcaaaccta aaaagataga tggattcctc ttttcctcct attgctttcc aattatcaat
     5221 ttcgccatca aatatttgtt taatttgagt tggatttaaa tcactaattg gatttttaga
     5281 gttaatataa agaccatagc cattttctac aactggagag cgaaataatc cgaaaccttc
     5341 tttaaataag aatattatta tgagcagtat aacaatacta gttattgaac tactcatttt
     5401 aaacacacct tctaatagcc tttcaataat tttcatagca gattttatat ttttattact
     5461 gaattttctg tctatttcaa tgtgatataa cctacattag aaacaatctt ttgaccttca
     5521 ggcgatagaa tatagtccac aaatcctttt actttattgc tatttttagt caaatagtaa
     5581 taatataatg gccttacaac tggataagtt ttattcttta cattagtgat attaggtaca
     5641 acataagttt ttcctttatc gtaagaaata tttattgttt tgacttctgt atttagatat
     5701 gcaaagccta catatccaat tgctccttta gtttgactaa ttgattgaat aattgctcca
     5761 gttgctggca aactcataat tccactcttg taattcttat ttttgagaac actttcttta
     5821 aaaaactcat aagttccaga agaggtttct ctagaataag gaactatttt taaatcatca
     5881 ccaccgactt ctttccaatt cgtaattttt cctgtaaaaa taccttctaa ttgttctcta
     5941 gttaatttag aaactttgtt tgaaggatta acaataacgc ttaacgcatc ataagcaatt
     6001 attatttctt ttaaagaatt gccagattct tgaattttta acttttcatc aaatttaatt
     6061 tttcttgatg cttgtgcaat ctctgttgtt ccactaatta aggctgagat tcctacacca
     6121 ctaccacctc cagttacaga gatagtactt gttggatttt tcttcttata attttcagca
     6181 gcttcttgtg ataacggcaa tacagtatct gaacctttga ttttttgagc ctttatgttt
     6241 aataccaaac agaataagga aattgttaaa ataattgttt ttttcatttt tattaattta
     6301 atttgtttta aaagggtttg ttttattttc agtgcaaatt tcaataccaa ttgttactgt
     6361 ctatttgcaa ttttgttaca atctggttaa gatttagaat ttatattgga atcgaagtgt
     6421 aaagacattg tcttttctat tttgattata atctgttata ttattagtag tttcatttct
     6481 aacaatgtca tagaatgctg taacacgaat actagaagtt gctttccata ccattcctaa
     6541 gccaaaagta ttataactga tatctccttt attggtttga tttaatccaa tattatcttt
     6601 tgaaacattc ttattaggat tataccaatc gtatttaaaa acgccagtta atggtacttt
     6661 tccaaaatct tgtgtaagca ttatataacc acctttaaat ttcctaatat aagtatctga
     6721 agttggtaaa gttgaagcat tggggctttt gctatcttta tttgttcctg gttgtgtacc
     6781 cattaaatat tctgccatta acttagttgt acctaccttt gttattgcat ttatttgcat
     6841 atcaaatcct atatattcac gttttgcata ttttcctata tttgttgagt cattatttaa
     6901 aacaaatgaa ttacctttca tcgtatagat attctttgag ccttgataaa cacctccatt
     6961 gtaataagat atcccaccac ctatttttag aatatcttta atcgtatgat taaaggataa
     7021 gtgtccaatg aagtcttttc tattatctgt ttccatttta atgccattcc ctgcaaagag
     7081 tccagcttct aatttgagta tattccaaat tgatgttttt ggagcttgta aggttagcat
     7141 taaacctaaa tctcgttcct ctggaaataa agtttggaaa attgttgagc gttcgggtgc
     7201 ttctctttgt gaagaggaat agctaatttc agacccaaaa ggtctatcaa aaaccccagc
     7261 ttttagtgcc atagatttta accaagggtc tttaatattt agataggcat cttttatccc
     7321 aattcctttt tcagtaatat caagttgaaa aacacccgca acaattcctt tttcataagt
     7381 aaattttatt ctgcctcttc taattccaac tcgattaatt gttttgtttt tgttgtcatt
     7441 tgaagctcca actttcagtg cagaaaattg ttctccgaat tgaaattgtg tttgaatata
     7501 gccagaaact ttaaagtttt tgttttcttt taatgtttct tcaatttcag taattctctt
     7561 ttctaaagca ttggaatcta ctggttctgt ttgtgaaaaa cttgctatac tcaatagtaa
     7621 gcaactcatc atcataattt ttttcttcat atcaaatatc taatttgatg caaagctatt
     7681 ttcaagtaat tacagaagga ttacagattt gttacaaact tttgactttt tatttaccat
     7741 ttatttttaa ataaaaaaac gtgcaaaata ttgcacgttt ctataaatgg ataacctatt
     7801 ttaaattatt tcaaagcttt ttcaatatca gcttttactt tattgtattg ctcaggtgtt
     7861 aaaatatttt ttattttatt atcagtttcg ctcaaaatct tttgattttc ttgattaaat
     7921 acttgaggat tagctttatt caccatttta tttgcatcag ccttaacttt tgcttcagct
     7981 aatgcaaaca tagattgaac aatttgtaca tcattcattt tgtaattgtt tctcattgcc
     8041 gatattaatt gctgttgttc agtcgtgaga ttttctccag tcaaaatatt tacttgtgct
     8101 ataataactc caaaaaatga atttgcttga gctggattta aggctgcttt aaattgatta
     8161 acaaacatcg gtgttttctt atctaattcc gatttctgtt gttttatatt atttggatac
     8221 attgcagaaa ctttggcaat ttcagcatca taattattgg ctactttaag tgcattagca
     8281 tattgagttg gtgataaatt caaagatgat tttagttgtg taagtgtctc tgctgactta
     8341 atatccgaaa gatttttatt attattgaca atagtagtat tatttgtagt attggtattt
     8401 gctacattta ctctaggatt ggtgtctttg cctagctttc cagttttaag taaagtttga
     8461 aacgcattat actgctctgt gcttaaataa ccctttgtgc tatttaatgt agaagctgtt
     8521 aattggtcaa actctgtttt tgctttaagt aaatttttgc cattagtctt agcaatactt
     8581 tctgttgtgg taacaaaatt aagataatcg ctactcattt ttccaaattg gttattgtct
     8641 aaaaccagct gcttaccaat attatccatc actgcttgat gttcctgtgg taaattatta
     8701 tagtctgcat tcttagtata agctgagttg gtattattac ttgttggtgt atttggtttt
     8761 gtattgacat tatcattttt tgaagcaggg ctttttaatg tttctacttt tgctgtcatt
     8821 tgaacttgtt tgttgaaata attgctatat cgattttgtt gaacgacagg tgttacttgc
     8881 tttaaattag cactagactg atttaaaacc attttaattt gatttacttt ctcttgtggt
     8941 gtaacattct tattaactat atcttgaatc gcaatacttt tagaggtgta ttcattagta
     9001 aaattcactc tttgagcagc tggtatttga agttccgtag caatattatt tgctgtttcc
     9061 tgaattctag acttactaac ttctttagcc aatacgatat tccctacact gaaaatacct
     9121 attaataatg tttgtttttt catataaaaa tttctccttg taaatataca aattttgtac
     9181 caaatttagt agaaaattca aactgcctag tcagactttt agaaatcaaa ttaaattaaa
     9241 atatgcattt tgagtattta gatttttata aatttgagca ttaatattat tacaaatgaa
     9301 atcttggcgt gtcttacaga aatacttaag tcttcaaatt catataaata aagaagaagc
     9361 agaaaatgaa acctaccttg aaataaaaga tggaactgtt tttaaaggat acaatctatg
     9421 gattttaggc tttgctatgg ttatagcttg tataggcctt actaccgatt ctttaagtgc
     9481 tgttattggg gcaatgctta tttcaccttt gatgggacct ataattggtt tggcttttgg
     9541 tttagccatt aacgacagag gattaaaaat tcaaggtata aagaattggc tcttaatgac
     9601 cttagtatgt ttggtttctg ctacaattta ttttttaatc aatccatttg ataacagtac
     9661 cgatatgtta gagactttca aacaggctat tatttttgat atattcttag cattttttgg
     9721 tggaatggct ggttttatag gtattataaa aaagaaggaa caaaaataat tgctggtgta
     9781 gccattgcta ctgcatgtat gcctccatta tgtactgctg cttatggtat cgcacattgg
     9841 gattataaat tctttcttgg cggattatat ttctatatta tcaattgtat gttcattgga
     9901 tgggctactt atctcttaac tagatatttt aatttcaaac aattattcaa taccaaacaa
     9961 aatcttcaat atgtcctttt atgggacatt ttaatcgtat ttatgttact ccctggttta
    10021 tacatcgcct ataataaatg gaaagctgaa gaaaataagg gcattcaagt ctcagacaaa
    10081 gttcgaattg aattattaga aaaaagattg gaaaaattag agaaatctat caatcgtcaa
    10141 taatttcttc agcataaaat atcctacagc ttcgtgattt tacattaaaa aattatccag
    10201 ataaaacaat tcctatttag agaaaaataa gaaagaactc ttgaaagtgt tattattata
    10261 atttataatt tatatttaaa attcgaatta aataaagcat taatcaaaca aacttaaatc
    10321 tttttccaag gcatttccaa taacaacaat atcagcacct gcattaaatg ctgcttgtat
    10381 ttgtgctttt gatttaatgc ctccgccaac gataagtggt acatcaattt gggctttgac
    10441 catattaata caatctgtac taattacaat ttctgcacca ctccctgctt ctaaatagat
    10501 acattttaag cccaattgag ttccagcaat cgcagtggct gcagcaattt caggcttatt
    10561 atttggaatt ggagtagtat tgctaatata gcttacgctt gttggcttgc caccatcaat
    10621 taaaatataa ccagttgata taatctctag cccactgttt tttaaaatag aagcaaattg
    10681 tacatgctgt cctattaata actcaggatt ccttccagaa attaaactca gcaataaaat
    10741 agcatctgcc ttatcacaaa tttgcattcc atttcctgga aaaataatta cgggtttgct
    10801 tgttaattgt tttaatgcaa gaatgatttt ttcaatatta tcttcattaa gtaaactgcc
    10861 tcctataaag taaaaatccg cagaagctgt tgcctcaact aattttttta aagatataac
    10921 atctgcttta tcaggatcaa taagggtagc tatttgcttt ttatcctgct taaataaatg
    10981 ataaatattg tttgatgcca ttttaacccc gaagatataa attttcttgt atttttgttg
    11041 aatatttcaa tttaacgctt ctacaaaatg actaacaaac atctatatac tttccttttt
    11101 gatgtgtttt cgctaatatt tgtttcgata ctttcttttc ttattctatt taatatgaaa
    11161 gaatggatga cccaaaaata ctacatcccc ttattcctgt attttacaat tgccgctttt
    11221 tattttagat gtattatatt tcctaaaagc tcttttttat ttagtttttg gccattttta
    11281 gttgtaattt ttcttggaaa tttttatatt acctttaaat tatttcacaa ttattatctt
    11341 ttcttagatg taattgatga ttattcaata cattttacca aagacttcgt tacaccatta
    11401 ataaaaggat tgacagcggg acaattattg tcaattaaaa gtatggcaac ttttggtttt
    11461 gtgttttata tggtaattat tgtgcttact caatttagaa ttatttggct gttactccgt
    11521 aaagcacgtg ctaaatttgc tagtaaagac agtaactatt aaactggaat tatttggata
    11581 tatttttcat atttcctgat ataattaatg atggctttct ttgtattatt aaattctttt
    11641 attggcttca ataagttatc taattcttga atatgttgaa tataggtggt ttcataaaat
    11701 gtataaccaa ctaaagcatt acttttaatt ttaatagcac atttttctga acccattctt
    11761 cctgcaataa ttatataaaa gctactaaat ggatattgat attttgccaa aatactttcc
    11821 attcgctcat tataatttaa tacggtatca atacgtttaa aaattggacc caatctcgaa
    11881 ccattaacaa tttggtgcaa cattttttca gctttcaact ttgaagtaaa tttcaaaatt
    11941 ggctcttctg cttcatctaa cacttttacg actaattttt caaacccatt ctcatctttt
    12001 gaggcaaata aaccaaatga aaattcataa tacttttgaa ttttattgta tttgggctga
    12061 taatgttgct tataatcttc ttctaacaaa atggctaaaa gttcatttcc caaaacttcc
    12121 acttgaatat ctgcagtttc tacatgcaat aattttttat atc
//
LOCUS       DAOQCW010000076        11830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_2034_length_11830_cov_5.427516, whole genome shotgun sequence.
ACCESSION   DAOQCW010000076 DAOQCW010000000
VERSION     DAOQCW010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11830
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2034_length_11830_cov_5.427516"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PLS16_07810"
     CDS             <1..500
                     /locus_tag="PLS16_07810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ61023.1"
                     /translation="WPHKNHIRVLEAISQLKSKELPFQIVFTGNTSSYRNENIFKDLK
                     NFIEKNQLIDDLIITGFIDRDEQIALIKNSIAVVQPSYFEGWSTVIEDAKALNKYVIA
                     SNINVNIEQINKDCTFFNPDNADELSKILAQFDIKYKDNNRKYNEDIQQFSEKLIKLF
                     SLNEA"
     gene            490..1929
                     /locus_tag="PLS16_07815"
     CDS             490..1929
                     /locus_tag="PLS16_07815"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNJ61024.1"
                     /translation="MKLKIIDIIKNNSFYDSLKKGSLLFFATIIVNIINFISIPIFTS
                     ILSVSDYGIIEVTNNYIRLFLILFPLNTISSISYFWYRKDIDNSKLITSVFFISLISF
                     SIISAVFYIFQHQIAEFFNIPLFAFQLIPPIIFFGIIFGIINAIFIYNSDVKLNALQQ
                     IFNTLLKVVASLLLIVLIFKDYKGRLIGEIVGYILLSVIFIKYLKDYIQFKLDLNYII
                     KIFTYSVSIILLSTSSFILNYFDTLMINNSLGNEKAGLYSYAYKISVIYFAFIQSFQV
                     IFNVKYATFFHDNKTENILSELKSLLKIVVLISGLFILLSKELGIILSINPEYEAALI
                     ICPIIVLGYFFCFIYDLYNIPLFHSKRIRNITIIIFICGGLNILLNKYLIPIYDYKIA
                     SVNTLISYLVMAILGYLFCRFSTNFYIRIKTLIPTISIILFYTITFYLLENLSLGFII
                     TIIIKLCILLISAIILFKNQIKNFINTNG"
     gene            1922..2737
                     /locus_tag="PLS16_07820"
     CDS             1922..2737
                     /locus_tag="PLS16_07820"
                     /inference="COORDINATES: protein motif:HMM:NF041524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 6 glucosyltransferase"
                     /protein_id="HNJ61025.1"
                     /translation="MDKIGILYICTGNYDIFFDTFYKSAEQFLLPDCEKHYFVFTESE
                     KISNTKFVHKIYQERLGWPNDTLMRFHLFLKIESELEQMDFLFFFNANYTFTKPILKN
                     EILPNLEDNDLVGQIHPVAYHKKSNEFAYENNQKSTAYIPTNKGKYYFAGGLIGGKTN
                     AFLQMCKQLKNDIDTDIKNNITAVWHDESHINKYFSEVEPKKLHPGFCYPEDWYLPFE
                     QKTLLLDKSKLGGHTFLRGEGKQSNKVAIKQKLFYFKLIIRAIFYKLLNYSPI"
     gene            2742..3626
                     /locus_tag="PLS16_07825"
     CDS             2742..3626
                     /locus_tag="PLS16_07825"
                     /inference="COORDINATES: protein motif:HMM:NF013681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,2-fucosyltransferase"
                     /protein_id="HNJ61026.1"
                     /translation="MIQVQLSGGLGNQMFQYAFGKHLAINTQSELVLDLAYIQSKLPF
                     KKFSTPMQYELDIFNLNVDCNSLYFSNGITYPLAKLEYYLKTKRNTNKNNTLIESGMQ
                     FNADYLEKDEPLFIQGNFQSELYFKDIEADIREDFEFIHPLKEQNQQILNQIKSSNSV
                     SIHIRRGDYLSIKNNASKFRALELDYYKNAMSILENKMENITYFVFSDDINWVKENLK
                     THAKTIFINHNKGKSSYIDMQLMSNCQHNIIANSTFSWWAAWLNANKNKIVVAPKTWF
                     LIDELNQNDIVPISWISL"
     gene            complement(3654..4586)
                     /locus_tag="PLS16_07830"
     CDS             complement(3654..4586)
                     /locus_tag="PLS16_07830"
                     /inference="COORDINATES: protein motif:HMM:NF013048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008417 - fucosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 10"
                     /protein_id="HNJ61027.1"
                     /translation="MKEIIKIDFSDFSDGISKQNNFIYNTLIQKYDVQISAQPDILFF
                     SCFGMNYLKYKNCKKVFWAGENLRPDFNGADYCITFDYNNNPNHYRYPLWVLSIIEKD
                     LIKIPTIEEVDEIVKSKNKFCAMLVSYCRASKRKELFNFISNNYKRIDSGGRCLNNMN
                     NQPIDNPLDFFNPYKFSLCFENSSHPGYSTEKIINAFRSNCIPLYWGDSELKNELNPK
                     RYISLHDFQNERTFLDYIIEVDNNNELYKSILKEPLLINGNIPPAYKKENFEQFLDKI
                     ITDKMPPDKTTLQKVKHFMGNYYFKYKWDRTWGF"
     gene            4702..7335
                     /locus_tag="PLS16_07835"
     CDS             4702..7335
                     /locus_tag="PLS16_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61028.1"
                     /translation="MSKNKKTEPLKQKSIQKSESSSSFEFLNSKAAKYIAIFAIVVIP
                     IFVFYFPLLEGKHIGTGDVVQGEAMSMKPWRDFQEKFGERNFLWNDQMFSGGPTTMTA
                     GTVFYAEWVKYTPINLIYNLAQKTIKDVTFNQIIFVGGAFLFFWVLFQNFWIALLFAI
                     AASWSNIAIMSVAAGHTNKQYVLASILPFWAGLILLLNKRYLIGLLVTTFFLKFLISS
                     FHIQLGYYSLIATLPFAGILIYQTIKEKDFKHLAISLGLVSISFMLSVGLNFNQIYIK
                     EYSDESNRGTNIIHIKDTSNTPTPLKAESGVDIDYATQWSFGWNELPSLFIPNYVGGS
                     SNAQLTPDSHIYKTLIGKGVPEGTAEQFIKQVPLYWGDEPFVLGRFYMGAVVLFLFFI
                     GIFAARKNKYILFWLLPMIVLTTLIALGKNSFGFYTLLFKYLPLFNKFRAPTMILGET
                     QMLFVIVGAIGMYYFLKKTELNERMDIFKKATFAAVGTIALFWMITFTQDFNNKTAAQ
                     NGNDDSYVSQLQQMTGNPEFANEIYTALVKDRKAANSSDTMRSLVFILLAAGIVYLLA
                     KKKLPDNAGLLLLTLICFVDFWGVNRREINNEVFVEQDDILNNTFVASPADNFILQDT
                     SDYRMIDFTVSPFNDASPAYFHKDIGGYSPAKLKRYQDVIEYGIQHDFSIINSQGLQK
                     ANFLNMLNTKYLKQTKEANGVIVNPYSLGNAWFVSNIIPVQTNEDEILKVRDINPRKD
                     VIIHDEFKNYYTNFTPNQDSSTQGRSIRKVSKHPMHLKYEFESPKDELVVFSEVVYKP
                     NVDWTSQIDGKPAEHIRANYILRAIKVPAGKHTITFDFMPKLYKPTYNIITASNVILE
                     ILFIGAIVGLFLNRRKKEENA"
     gene            7328..8242
                     /locus_tag="PLS16_07840"
     CDS             7328..8242
                     /locus_tag="PLS16_07840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ61029.1"
                     /translation="MLNTFSFIVISYNRAKDTIDALKNILWLDNVEGWKKEIIILNNN
                     SSEDYLPLEHYIAQCSIEIQKTIHYIHHNENLGVSGGRNFCIKKATGKYLFFLDDDAE
                     IVQQNAIAIILKKFEQYKSENLGIIGFLGKNPYNNTYQSPIKNQALIKDKNEIFYNLF
                     YGYGHVFPKALIENTGYYQEDFFYGMEENDLSYASIKKGYAILFSKDILVLHKVNPNG
                     REPNIQTKSRLFQNRMIVVYKHLPFIYVLTQFIMWSFYFLYHSKINIIAYFKALIQLK
                     QRIKLVQRKPMNQKGMAYLRKVQARLWY"
     gene            8251..9108
                     /locus_tag="PLS16_07845"
     CDS             8251..9108
                     /locus_tag="PLS16_07845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ61030.1"
                     /translation="MIEGKNILITGANSGIGKVTAIELAKKGANIIMVCRNKEKAQIA
                     QQEINTSSKLNNTDLLIADLSSSESIRRFADEFYKKYDKLDVLINNAGAIYTNRQENK
                     EGIELTMATNHLGYFLMTHYLLDALKNTDTARIVNVASVAHRFTNYKPNDLNAQKSYQ
                     YMKQYGLSKLSNMLFSKYLSQYLKDTNITTNALHPGNIASNFGKNNNSFFGYLVKIGA
                     FALINPNKGALTSIYLASSPEVEGKTGLYWYKQKEEKPSSDAINPEYAQHLWNWSLDK
                     TKIQTFGKI"
     gene            complement(9121..9417)
                     /locus_tag="PLS16_07850"
     CDS             complement(9121..9417)
                     /locus_tag="PLS16_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61031.1"
                     /translation="MSKFLKSPMFNGIMLGLIVPIIVFFIWHQLKYSAMDLSSFIDMT
                     LTKVHVPATIRMCVFANLPLFLLYNFIQRFEVCIGIFISSMFYIIVMAGFYFFG"
     gene            complement(9410..10186)
                     /gene="surE"
                     /locus_tag="PLS16_07855"
     CDS             complement(9410..10186)
                     /gene="surE"
                     /locus_tag="PLS16_07855"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNJ61032.1"
                     /translation="MQRPVILVSNDDSINAPGIKALVEVAKKFGDVVVVAPDSPQSGM
                     GHAITIHDPLRLHKVEVFEGVESYQCSGTPADCVKIAIDKVLYRLPDLCISGINHGSN
                     ASINVIYSGTMSAAMEAAISSVPAIGFSLLDFSFDADFEASKHFAEIVIREFFNNGLP
                     DACLLNVNIPKLPLEEIKGMKVCRQAVAKWEEEFEKRVDPNGNEYFWLTGNFVNYDKG
                     EDTDVWALENNFVSIVPVQYDLTNYQGITHINKNWNISNE"
     gene            complement(10494..10739)
                     /gene="atpC"
                     /locus_tag="PLS16_07860"
     CDS             complement(10494..10739)
                     /gene="atpC"
                     /locus_tag="PLS16_07860"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HNJ61033.1"
                     /translation="MQLVVLTPEKEVYKGEATLVHLQGVDGQFQIMDKHAPLIAALSD
                     GLLKITNNKEKLSFFIKGGIVEVLNNKVSVLTEGIEE"
     gene            complement(10765..>11830)
                     /gene="atpD"
                     /locus_tag="PLS16_07865"
     CDS             complement(10765..>11830)
                     /gene="atpD"
                     /locus_tag="PLS16_07865"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNJ61034.1"
                     /translation="GKIGLFGGAGVGKTVLIMELVNNIAKGHGGLSVFAGVGERTREG
                     NDLLREFLESNVIKYGEKFAHSMENGDWDLSLVDKEQLKESKATLVFGQMNEPPGARA
                     RVALSGLTIAEQFRDEVSDGKGKDILFFVDNIFRFTQAGSEVSALLGRMPSAVGYQPT
                     LATEMGLMQERITSTKSGSITSVQAVYVPADDLTDPAPATTFAHLDATTVLSRKIAEL
                     GIYPAVDPLDSTSRILDPQVIGERHYAVAQRVKMILQRYKELQDIIAILGMDELSEED
                     KLVVHRARRVQRFLSQPFHVAEQFTGLKGVFVSIEDTLKGFEMIMDGEVDEYPEAAFN
                     LVGSIEDAIEKGKKILAEAK"
BASE COUNT         4309 a         1829 c         1649 g         4043 t
ORIGIN      
        1 tttggccaca caaaaatcac atcagagttt tagaagcaat tagtcaatta aaaagtaaag
       61 aactgccttt tcaaattgtt tttactggca atacttcatc gtatcgaaat gaaaatatat
      121 ttaaagattt gaaaaatttc atagaaaaaa atcaacttat agatgaccta ataattactg
      181 gatttataga tagagatgaa caaattgcct taataaaaaa ttcgattgca gttgttcaac
      241 catcatattt tgaaggttgg agtactgtaa ttgaagatgc taaagctttg aataaatacg
      301 ttattgcttc aaatataaat gttaatatag aacagataaa taaagattgt acttttttca
      361 atccagataa tgcagatgag ttgtccaaaa ttttagctca atttgatatt aaatacaaag
      421 ataataacag aaaatacaat gaggatattc aacagttttc agaaaaatta attaaattat
      481 ttagtctgaa tgaagcttaa aattatagat atcatcaaga acaatagctt ttacgattca
      541 ctaaaaaaag gctctttgtt attttttgca actataattg tgaacattat aaattttata
      601 agcataccta tttttacgag tattttatcg gtaagcgact atggaattat tgaagtaacg
      661 aataactata ttcgactttt tttgatactt tttccactaa ataccatatc ctctataagt
      721 tatttttggt atcgaaaaga tattgataat agcaaattaa ttacctctgt tttctttatt
      781 tccttgattt cgttttctat tattagtgcc gtattttata tatttcaaca ccaaattgca
      841 gaatttttca atattccatt atttgcattt caactcattc ctccaattat cttttttgga
      901 attatttttg gtattatcaa tgccattttt atttacaatt ctgatgttaa actcaatgcc
      961 ttacaacaaa tttttaatac tttactcaaa gttgttgcat ctttactttt gatagttttg
     1021 atctttaaag attacaaagg taggctaatt ggagaaattg tgggttatat tttattgtct
     1081 gttattttta taaaatatct aaaagattat atccaattta agctcgacct aaattatatt
     1141 ataaaaattt tcacctattc agtttctatt attttactca gtacaagtag ctttattttg
     1201 aactattttg ataccttaat gattaataat tccttaggaa acgaaaaagc tggtttatat
     1261 agttatgcct ataaaataag tgtgatttat tttgctttta ttcaatcttt ccaagttatt
     1321 tttaatgtaa aatatgctac ttttttccac gataataaaa ctgaaaatat attaagtgaa
     1381 ctaaaaagtc tattaaaaat agtagttcta atttctggac ttttcattct attatctaaa
     1441 gaattaggca ttattttatc cataaatcca gaatatgaag ccgcactcat tatttgccca
     1501 ataatagtat taggctattt cttttgtttt atctacgatt tatacaatat tccacttttt
     1561 cattcaaaaa gaataagaaa tataacgatt atcatattca tttgtggtgg cctaaatatt
     1621 cttttaaaca aatacctaat tccaatttat gattataaaa ttgcaagtgt aaacacctta
     1681 atttcttatt tagttatggc aattttaggt tatctattct gccgattttc aacaaatttt
     1741 tacattcgca taaaaacatt aattcctaca atcagtatta tattatttta taccattaca
     1801 ttttacttac ttgaaaattt aagtttaggt ttcatcatta ctataattat caaactatgt
     1861 atattactta ttagtgctat aatattgttc aaaaatcaga taaaaaattt cataaacacc
     1921 aatggataaa ataggcattt tatatatatg cacaggaaat tatgatattt tctttgatac
     1981 tttttacaaa agtgcagagc agtttttatt acctgattgc gaaaaacatt attttgtttt
     2041 tacagaaagt gagaaaattt ctaacacaaa atttgtacat aaaatttatc aagaaagatt
     2101 aggttggcca aatgatacct taatgcgatt tcatctattt ttaaaaattg aaagcgaatt
     2161 agaacaaatg gattttctgt ttttttttaa tgccaattat acttttacta aaccaattct
     2221 aaaaaatgaa attcttccaa atttagaaga caatgattta gttggtcaaa ttcatccagt
     2281 tgcttaccac aaaaagagca atgaatttgc ttatgaaaac aatcaaaaat ctacagctta
     2341 tattccaacc aataaaggca aatattactt tgctggtgga ctaattggag gaaaaacgaa
     2401 tgcatttttg caaatgtgta agcaactaaa aaatgatata gatactgata taaaaaataa
     2461 tattacagca gtttggcacg acgaatcaca tattaataaa tatttttctg aagttgaacc
     2521 aaagaaatta catccaggct tttgctatcc tgaagattgg tatttacctt ttgagcaaaa
     2581 aacattatta ttagataaaa gtaaattagg aggacatact tttttgagag gcgaaggaaa
     2641 acaaagcaac aaagttgcta tcaaacaaaa actattttat tttaagttaa ttattagagc
     2701 aatcttttat aaattgctaa attactctcc aatttaatac aatgattcaa gttcaacttt
     2761 ctggaggatt gggcaaccaa atgtttcaat atgcttttgg gaaacattta gcaataaata
     2821 cgcaatcaga actagtttta gatttagcat acattcaaag caaattacct tttaaaaaat
     2881 ttagtacacc aatgcaatat gaattggata ttttcaactt gaatgtagat tgcaattcac
     2941 tctatttttc aaatggtata acttatccac ttgcaaaact agaatattat ttaaaaacca
     3001 aacgaaatac caataaaaac aatactttga ttgaatctgg aatgcaattt aatgctgact
     3061 atttagaaaa agacgaacca ctttttatac aaggtaattt ccaatctgaa ttgtatttta
     3121 aagacattga agcagatatt cgtgaagatt ttgaatttat tcatccatta aaggaacaaa
     3181 atcaacagat tttaaaccaa ataaaatcca gcaattccgt atcaatacat attcgtcgtg
     3241 gagattattt atctatcaaa aataatgctt caaaatttag agcattagaa ttagattatt
     3301 acaaaaatgc gatgtctatt cttgaaaata aaatggagaa catcacctat tttgtatttt
     3361 ccgacgatat taattgggta aaagaaaatt taaaaaccca tgctaaaaca atatttatca
     3421 accataataa aggaaaatca agctatatcg atatgcaatt aatgagcaac tgccagcaca
     3481 acatcattgc aaatagtact tttagttggt gggcagcttg gttaaatgca aataaaaata
     3541 aaatcgttgt cgcaccaaaa acttggtttt taatagatga attaaatcaa aacgatattg
     3601 ttccaataag ttggataagt ttataaaaga caacaaccaa tatggctttc aatttagaaa
     3661 ccccaagttc tatcccattt atatttgaaa tagtaattgc ccataaaatg ttttaccttt
     3721 tgtaaagtag ttttatcggg tggcatttta tctgtgatta tcttatctaa aaattgttca
     3781 aaattctctt ttttataagc aggtggaata tttccattaa ttaataatgg ctctttcaaa
     3841 atacttttat acaattcgtt attattatct acttcaataa tataatctaa gaaagtacgt
     3901 tcattttgaa aatcgtgtag cgaaatgtaa cgtttcggat ttaactcatt tttcaattct
     3961 gagtcgcccc aatacaatgg aatacaatta ctacggaatg cattaattat cttttcagta
     4021 gaatatcctg gatgagaaga attttcaaaa cacaatgaaa atttataagg attaaaaaaa
     4081 tctaatggat tatcaatcgg ttgattgttc atattattca aacatcttcc gccagaatct
     4141 attcgcttat aattattact tataaaatta aataattcct ttcgtttgct tgctcgacaa
     4201 taagaaacca acatggcaca aaatttattt ttgcttttca caatttcatc tacttcttca
     4261 attgttggaa tttttattaa atctttttct attatactaa gcacccaaag tggatagcga
     4321 taatgatttg ggttattgtt ataatcaaat gtgatgcaat aatcggcacc attaaagtct
     4381 ggtctaaggt tctctcctgc ccaaaatact ttcttacaat tcttatattt caaataattc
     4441 attccaaagc atgaaaaaaa taaaatatca ggttgtgctg agatttgcac atcatatttt
     4501 tgtatcagtg tattgtaaat aaaattattt tgtttactaa tcccatcact aaaatctgag
     4561 aaatctattt taataatttc tttcattttt accttgtaaa atctaaaata tcccttttaa
     4621 taggaagaac aaaaataaca tctataaacc taaatttgta aatttaaaat attatttttg
     4681 aaacaataaa atacttaaaa aatgtcgaaa aataaaaaga cagaaccttt aaaacaaaag
     4741 tctattcaga aaagtgaatc gagttcatct tttgaattcc taaactcaaa agctgcaaaa
     4801 tacattgcaa tttttgccat cgttgttatt cctatttttg tattttattt tccattacta
     4861 gaaggaaaac atattggaac tggcgatgtg gttcaaggcg aagcgatgtc tatgaaacct
     4921 tggcgtgatt ttcaagaaaa atttggggaa agaaatttct tatggaatga ccaaatgttt
     4981 tcaggtggtc caaccacaat gacggctggt acagttttct atgcggagtg ggtaaaatac
     5041 acaccaatta atctcattta taatttagct caaaaaacaa ttaaagatgt aacttttaat
     5101 caaattattt ttgttggagg tgcatttcta ttcttttggg tattatttca gaatttttgg
     5161 attgcattac ttttcgcaat tgcagcgagt tggagtaata ttgccattat gagtgttgcc
     5221 gctggacaca ctaataaaca atatgtttta gcaagtattc ttccattttg ggctggttta
     5281 attttattgc tcaataaacg atatttaatt ggtttattag ttactacttt cttcttgaaa
     5341 tttcttatca gtagttttca tattcaatta ggatattata gtttaattgc cacactccca
     5401 tttgctggaa tattaattta tcaaaccatc aaagaaaaag attttaaaca tttagcaatt
     5461 agtctaggat tggtttccat atcttttatg ctcagtgttg gtctaaactt caaccaaatt
     5521 tatatcaaag aatattccga tgaatcgaat cgtggaacca atattattca tataaaagat
     5581 actagcaata caccaacacc gctcaaagct gaaagtggcg tagatattga ctatgcaaca
     5641 caatggagct ttggctggaa tgaattacca tctttattta ttccaaatta tgtaggtggt
     5701 tcttcaaatg cccaacttac gccagattca catatttata aaacactaat tgggaaaggt
     5761 gtgcctgaag gaacagcaga acagtttatc aaacaagttc ctttatattg gggagatgaa
     5821 ccttttgttt taggtagatt ttatatgggt gcagtggtgc ttttcttatt ctttataggc
     5881 atatttgcag ccagaaaaaa caagtatatt ttattttggt tattaccaat gattgtttta
     5941 accactttaa ttgcattggg taaaaactcc tttggatttt atacattatt attcaaatac
     6001 ttaccacttt tcaataaatt tagggctcca acaatgattt tgggcgaaac tcaaatgcta
     6061 tttgtaattg taggtgcaat tggaatgtat tatttcttaa agaaaacaga gttaaatgag
     6121 cgaatggata ttttcaaaaa agcaacattt gcagcagttg gcacaatagc tttgttttgg
     6181 atgataacat ttacccaaga tttcaacaac aaaacagctg cacaaaatgg taatgacgat
     6241 agttatgtaa gtcaattaca acaaatgact ggtaaccctg aatttgcaaa tgaaatatac
     6301 acagctttag taaaagatag aaaagcagct aattcgagtg atactatgcg ttcacttgtt
     6361 tttattttat tagcagcagg tattgtttat ttattagcca agaaaaaact tcctgacaat
     6421 gctggtttat tacttttgac cttaatatgt tttgtagatt tttggggtgt aaatagacgt
     6481 gaaataaata acgaagtctt tgtcgaacaa gatgatattt tgaataatac atttgttgca
     6541 tctccagcag ataattttat tttacaagat acaagcgatt atagaatgat tgattttaca
     6601 gttagtccat ttaacgatgc atcgcctgct tattttcata aagacattgg tggttactct
     6661 ccagctaaat taaaaaggta ccaagacgta atagaatatg gtattcaaca cgattttagc
     6721 atcatcaaca gtcaaggttt gcaaaaagca aatttcttga atatgctcaa tacaaaatat
     6781 ttaaaacaaa ctaaagaagc aaatggcgtt attgtaaatc catatagttt aggaaatgct
     6841 tggtttgttt ctaatataat tcctgtgcaa accaatgaag atgaaatctt aaaagttcgt
     6901 gatattaatc caagaaaaga tgtcatcatt cacgatgaat ttaaaaatta ctatacaaat
     6961 ttcacaccca accaagacag ttcaacacaa ggaaggtcta taagaaaagt gagtaaacat
     7021 ccaatgcatt taaaatatga atttgaatct ccaaaagatg aattggtggt attttctgaa
     7081 gtagtttata aaccaaatgt agattggact tcacaaattg atggaaaacc agctgaacac
     7141 atcagagcaa actatatttt gagagctata aaagtacctg ctggaaaaca taccatcact
     7201 tttgatttta tgccaaaatt atataaaccg acttataaca ttattacagc ttcaaatgta
     7261 attttagaaa ttctattcat tggtgcgatt gtaggtttat ttttgaatag aagaaagaaa
     7321 gaagagaatg cttaatactt tctcatttat agtcataagt tataacagag caaaagatac
     7381 aattgatgct ttgaaaaata tcttatggct agataatgta gaaggttgga aaaaagaaat
     7441 aataatttta aataataatt catctgaaga ctatcttcca ttagaacatt atattgcaca
     7501 atgttcaatt gaaatacaaa aaacgattca ttatattcac cataatgaga atttaggtgt
     7561 ttcaggaggt cgtaattttt gtatcaaaaa agcaacagga aaataccttt ttttcttaga
     7621 tgatgatgca gaaattgtac aacaaaatgc tatcgctatc atattaaaaa agtttgagca
     7681 gtataagtct gaaaacctag gaataatagg ctttttagga aaaaatccat ataacaacac
     7741 ctatcaaagt cctataaaaa accaagcttt aataaaggat aaaaatgaaa tattttataa
     7801 ccttttttat ggatatggac atgtttttcc aaaagcgtta attgaaaata ctggctatta
     7861 tcaagaagat ttcttttatg gtatggaaga aaatgattta tcttatgcat caataaaaaa
     7921 aggatacgct attttattta gcaaggatat tttggtattg cataaagtca atccaaatgg
     7981 aagagaacca aatattcaaa cgaaatcaag gttatttcaa aatagaatga tagtcgttta
     8041 taaacatctt ccatttattt atgtacttac tcaatttatt atgtggagtt tttatttttt
     8101 ataccattcc aaaataaata taatcgctta tttcaaagca ttaatacagt taaaacaaag
     8161 aataaaatta gttcaaagaa aaccgatgaa tcaaaaaggt atggcatatc tccgaaaagt
     8221 tcaggcacga ttatggtatt aaattaaatt atgattgaag ggaaaaacat attaattact
     8281 ggagcaaatt ctggaattgg aaaagttacg gcaatagaat tagctaaaaa aggggcaaac
     8341 attattatgg tttgtcgaaa taaagaaaaa gcacaaattg cacaacaaga aatcaataca
     8401 agttctaagt taaacaacac cgatttattg attgcagatt taagttcatc agaatctata
     8461 cgtcgatttg ctgatgagtt ttataaaaaa tatgataaat tagatgtttt aatcaataat
     8521 gctggagcta tttatactaa tagacaagaa aataaagaag gcattgaatt gactatggcg
     8581 accaatcatt tgggctattt tcttatgaca cattatttat tagatgcact aaaaaataca
     8641 gatacagcac gtattgtcaa tgtagcttct gtagctcatc gttttaccaa ctacaaacct
     8701 aatgatttga atgcacagaa aagctatcaa tatatgaaac aatatggttt gagcaaattg
     8761 tccaatatgc tattttcaaa gtatttgagt caatatttga aagataccaa tatcacaaca
     8821 aatgctttac atcctggcaa tattgcttct aattttggaa aaaataacaa ttcctttttt
     8881 ggctatttgg taaaaattgg agcctttgca ctcatcaatc caaataaagg tgcactaacg
     8941 agtatttact tggcaagttc tccagaagtt gaaggaaaaa cgggtttata ttggtacaaa
     9001 caaaaggaag aaaaaccttc tagtgatgcc ataaatcctg aatatgcaca gcatttatgg
     9061 aattggagtt tagataaaac taaaattcaa acctttggta aaatataaga ttattaagaa
     9121 tcacccaaag aaataaaaac ctgccattac tataatatag aacattgaac taataaaaat
     9181 cccaatgcaa acttcaaatc tttgaataaa attatacaat agaaataaag gtaaatttgc
     9241 aaaaacacac attctaattg ttgcaggtac atgaactttt gttagtgtca tatctataaa
     9301 actactcaaa tccattgcac tgtatttcaa ttgatgccag ataaaaaaaa caataatcgg
     9361 aacgattaaa cccaacataa ttccattaaa cattggggac tttaaaaact tactcattgc
     9421 tgatattcca atttttatta atgtgtgtaa taccttgata attggtcaaa tcatactgga
     9481 ctggcactat agaaacaaaa ttattttcta atgcccaaac atcggtatct tctcctttgt
     9541 catagttcac aaaatttcct gtaagccaaa aatattcatt gccatttgga tctactcttt
     9601 tttcaaattc ttcttcccat tttgcaactg cttgacgaca tactttcatt cctttaattt
     9661 cctcaagcgg taattttgga atatttacat ttaataaaca agcatcaggt agtccattat
     9721 taaaaaactc tctaataact atctccgcaa aatgttttga tgcttcaaaa tcggcatcaa
     9781 atgaaaaatc taataatgaa aaaccgattg caggtacact agaaattgct gcttccatag
     9841 ctgcagacat ggttcctgaa taaatcacat ttatagaagc atttgaacca tgattaatac
     9901 cagaaataca caaatctggt aatctatata aaactttatc aatagcaatc ttcacacaat
     9961 ctgctggagt tccagaacac tgataagatt caacaccttc aaaaacctcc actttgtgca
    10021 gtcgtaaagg atcgtgtatg gtgatagcgt gtcccatacc agattgggga ctgtctggtg
    10081 cgacgaccac cacatcacca aatttctttg ccacttcaac taaggcctta atgcctggag
    10141 catttatact atcatcatta ctaactaaaa taactggtct ttgcatattg tacaaagcta
    10201 taaaaataaa tctgtccaat agattaattt atgcaaaaat tcaaatcaat tttaacccga
    10261 attatgcaat aggattcgtt atgaaagtga aaataccaat aaaatataaa cttttgcgat
    10321 tcaaacatag caacctctat ggtgaatgag caaaagttag cgtagcgtta tattttgcag
    10381 gcgttttcga ttgtaataga atatctattg cataattcgg gtttaaaaat caacaccata
    10441 aaaaaaggga taatctaacg actatccctt ctaccttatc tatcaataaa gaattattct
    10501 tcaatacctt cggtcaaaac agaaacttta ttattcaaaa cttctacaat gccacctttg
    10561 ataaaaaagc tcaatttttc tttattgttt gtaattttta ataatccatc agaaagtgca
    10621 gcaattaatg gagcatgttt atccataatt tggaattgtc catcaacgcc ttgtaaatgt
    10681 acaagcgttg cttcgccttt ataaacttct ttctccggtg ttaatacaac taattgcatg
    10741 tttacttctt ttgatatttt ccaattattt agcttctgct aaaattttct tacctttttc
    10801 gattgcatct tcaattgaac caaccaagtt gaaagctgct tctggatatt catctacttc
    10861 accgtccata atcatttcaa agcctttcaa agtatcttcg atagaaacga atacaccttt
    10921 caaacctgta aattgctcag caacatggaa tggttgagat aagaaacgtt gtacccttct
    10981 tgctctgtga actactaatt tatcttcttc agaaagctca tccataccta agatagcgat
    11041 gatatcttgt aattctttgt aacgttgtaa aatcattttt actctttgag caacagcgta
    11101 atgtctttcg ccaataactt gtgggtctaa gattcttgaa gtagaatcca atggatctac
    11161 tgcaggataa attcctaatt ccgcaatttt acgggataat acagtggttg catctaagtg
    11221 agcaaatgtt gttgctggag cagggtcagt taagtcatct gcaggtacat aaactgcctg
    11281 aacagaagta attgaaccac tcttagttga tgtaatacgc tcttgcatca aacccatttc
    11341 tgtagccaaa gttggttgat aacctaccgc agatggcata cgacctaata atgcagatac
    11401 ctcagaacca gcttgtgtaa aacggaagat attatccacg aagaataaga tatctttacc
    11461 tttaccatca gaaacctcat ctctaaattg ctcggcaatt gtcaaaccag ataaagcaac
    11521 acgagctctc gctcctggtg gctcattcat ctgaccaaaa actaaagtag ctttagactc
    11581 ttttaattgt tctttatcaa ctaatgataa atcccaatct ccattttcca tagagtgagc
    11641 aaatttttca ccatatttaa taacgttaga ttctaagaac tcacgtaata agtcatttcc
    11701 ttctcttgta cgctctccaa caccagcaaa tactgataaa ccaccatgtc ctttagcaat
    11761 attatttacc aattccatga ttaataccgt tttgccaaca ccagcaccac caaacaaacc
    11821 aattttacca
//
LOCUS       DAOQCW010000077        11523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_2109_length_11523_cov_5.643791, whole genome shotgun sequence.
ACCESSION   DAOQCW010000077 DAOQCW010000000
VERSION     DAOQCW010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11523
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2109_length_11523_cov_5.643791"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3117
                     /locus_tag="PLS16_07870"
     CDS             <1..3117
                     /locus_tag="PLS16_07870"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ61035.1"
                     /translation="NKFYTFMRKYSLLFIVVSICFNVFSQASLKHTDILKMYYEAKEQ
                     IAYERYAIAYPIIQDFLKNYENNVLKIDPSIVSESYFYKALCAKETKAPEAQTLLIDY
                     IEDYPGTTHINTARFLLGEIAYDIPNYQNAIEYFKEVNEKELSKNQALDYNYKYAFAL
                     FATKKFKEARARFNTLTASDSKYKEDAFYYSGLSSYYLGDYNNAYKTFQKIETAKKYD
                     KIVPYYLASIQFVNKDYKGVVAYAEPKLKENVKYTTEIAHLLGNSYYELQQYDKAKYY
                     LEEYMSKSVKATPEDYYILGYVQAQENDCKNAVKNLGELSPLETELAQNAMYQMAKCL
                     IKIDKKNDAKNAFLQSARLDFNKAIKEESIFQYAKLSYELNNINDALVTLKKFIVDYP
                     KSIYYNEANELLADIFLITKNYDEAIAIIESLPNVSPKLQATYQQMAYLKALSLYNDK
                     NYAQAIPFFNKSLKYTSNKSYEALTYYWKADIAHQSGDYTQSKQLLDKFFSLSKNINT
                     AENIMVSNGTANYLQGYNYYKEKNYLQAVVNFSAALNQLKDSKTTSIAQNIYPDALVR
                     LADAYFIQKKYQDAKQNYDVVIKNNFKAADYALFQNAIIDGLEGKYLDKIAGMNALVA
                     RFPKSSFADEALFQAGNTQFAISKTNDAKQTFNTLLKNYPKSTRVPDTYNKLGLIAYN
                     QDSYDEALSWYQKVVDKYPNTPAANEAMLVIKDIYIDKGDANGFIKYASKNSSSNLTA
                     SAQDSIIYLTAESQFQKGNIKQALDGFDSYLLQYPKGFFNLQARFYRAECNYSLQSFD
                     KALQDYVYVANLPQNRFSERSVARSANIFYFDQKNYKEALNYYSKLEQIATLDENKRE
                     SLIGLMRTNFKLNNHTSTLVYTDKVSELSNLPEFYKREITYYKGVTYFKQQDYDKALP
                     QLQTIATTLDNEQSAEAQYDIAKIYFEKKNYKQAESEAMKYTENFPAYEYYLGKTYIL
                     LSDIYKVNGKILQAKATLQSLIDNYSFDDDVMKEAKEKLQIIIDQETQNSNLKLPDNN
                     LQILQFDK"
     gene            3142..4842
                     /locus_tag="PLS16_07875"
     CDS             3142..4842
                     /locus_tag="PLS16_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61036.1"
                     /translation="MRFNNNIYLFKQAFILISISFFSSFDALAQKATDTIYDLDVVNI
                     IQSYEPILINSDKIPLLPDVPKFSKNKPDPQTYQFSDVKGKINYSAEDIRPIKMSLPQ
                     ADKQKFLYLKAGFGNYLTPLLNLSLVNPNQSKFRAGLNTDFIFSKAKKPRFQQYFDFK
                     AKGFGEYFIKSSALVGASINTQFDQYHFYANQDSNITRKDIANMYKRFGANIYLKNIA
                     DKDFDYQANIGFNNTSNTLNQKETTIQIDLKGHYMFMQNIGAGANILVNNVGYKDAID
                     SNKTNRFSMGINPYAIVQYKIWKLKLGANVMGTNKKLYVLPSIQNVLNIYKNYLTMYN
                     EWNSQVKVNSLHKNSLENAFIDANNFRNQIDEKRTFIGLRGAASGFYYDLNFSQLVSR
                     NALLYAYNEYDNTYDAAISPRIGTFQPEYIHKIKALNPHIALGYAKGNKYGAKLSFDY
                     YIYNKNNDFEVIYKPKLQSKLSVFYNWNEKLFIDLSMTAYGKMNGLVHQVSNDVIVNT
                     YLQPIKGVVDINLSASYFFTKNIGVFLDLTNISHQKYQHYINYPSYGVQVIGGVKIAY
                     "
     gene            4854..5954
                     /locus_tag="PLS16_07880"
     CDS             4854..5954
                     /locus_tag="PLS16_07880"
                     /inference="COORDINATES: protein motif:HMM:NF036727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61037.1"
                     /translation="MNAFITQLLYQNNCVIVPKVGAFLANYKSAFVDMHQNIILPPQK
                     TIAFNKQLNKSDGLLAAFVSEQKMISYTAAEQLVNQFGDEILFSLEKHKSFIFSGIGR
                     LYKDASDNILFESFGQENFLIENYGLLPVAIEPISRLKSEAVIEPIVPTVAVDEKQVS
                     VSNKKSGIRWVLPVIIPFLLITIIGFFVMFSQFNPQFSKFDLASLVPNVKQLFEQKNQ
                     QNILKTEYPKATIPTQKDTTHLVVKANIDSVLINTSTSVAVDTITPIKKEVVQTEKPV
                     EVKEPEKKPIVLENGKVANAFICVGSFKNESNAKEMQKMIDFLGLQSVVSKTKLHQVL
                     VATSTARAGEDLATLKEEIAPGAYIYCQNCNY"
     gene            5960..6613
                     /locus_tag="PLS16_07885"
     CDS             5960..6613
                     /locus_tag="PLS16_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ61038.1"
                     /translation="MISCSDIHKSYGQLEVLKGINLQVNQGELVSVVGASGAGKSTLL
                     HIIGTLDKPDRGEVIINGMSVANLKDKALSEFRNKNLGFIFQFHHLLPEFTALENVCI
                     PGYIANRKDAEVQNRAKELLDFMGLKDRFHHKPNELSGGEQQRVSVARALINNPLLIL
                     ADEPTGNLDSEKSEELHNLFFDMKRMFNQTFIIVTHNEHLAEKADKKIVMKDGLIIS"
     gene            complement(6614..7495)
                     /locus_tag="PLS16_07890"
     CDS             complement(6614..7495)
                     /locus_tag="PLS16_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61039.1"
                     /translation="MKNLLFLFVFFTFFALNAANNESIIFIRNAKTLPNINNKGVYGI
                     DFNYEIDFKALEDKISNDSIMDLSKFTLNITFSENGSKIKPALGYQKLKNKNGNLTLI
                     KKYDSFDFGSNYPIKESLFIPLAAFNLSEGEHTINVLVGLNGTDALGNKYQQVIIEKN
                     IVLKKKPTHKITFNIDYLEVNKLMSNGQTWDAGFYNTLAPDVSVKIMLANTIIWKEAV
                     RDDYIFAKGPKSKNIQFLVSTDDEIRFLVEDEDIVFNDFIGELKIPVYDTQIGKQFTL
                     NKQFNAVIDCVASYKVE"
     gene            7701..8942
                     /locus_tag="PLS16_07895"
     CDS             7701..8942
                     /locus_tag="PLS16_07895"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNJ61040.1"
                     /translation="MEQQEQQAAQQKKIKKEYFAVVDGLRGASMCFTVLVHMNLTIFK
                     LPAFLAHFGLHGFYVLSAFLISTILYKEKVKFGEFKPYAKNFYIKRFLRIIPLYYAYI
                     IGIAFIGLILKFITHGDPFGIIYELKNFGINLFTFTYNYKDLFAFLKGLDHQKCNLFP
                     HLWSISIEEQFYLVIPAVLFFLTKEQVKKMAIAIIIIYPFFRVAGFLYLRDVLHMTST
                     HGKHEYEIMYNFFYRSSLFQVDAFMYGLLIPLVNYNNRKVLRWIVIGSTALIFVGQIY
                     NMFDYAHEQGVSAWSVVGEHVVVMKNGMYAYVNTLYNILASSLIYYLLKFPDSSLNKI
                     LKFPFFMEWGKIVYGIYVYHMIFVTITAIIFYTVLKVYLPLPIAELLYIALCFTSVYY
                     FSKFSFYKFEMYFLNIKNKRD"
     gene            9016..10497
                     /locus_tag="PLS16_07900"
     CDS             9016..10497
                     /locus_tag="PLS16_07900"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HNJ61041.1"
                     /translation="MKNINYIKILLVLLTVSIFSRINAQHICGQDIITHQLRSLSSDY
                     DNDYSNLLEEWQYEKQNARTSALEDTTYTIQVVVHVVYLEDNLIQNIPDSIIHSQIDA
                     LNRDFGVTNEDTVNLRPIFKPFRGYPKIKFELAKLDTSGNPTTGIIRVAGKAPSANAV
                     FTNPLDSILSNLLNAVGDWFKKGYTISTLAGNVKDTTIGSPIWDNKKYLNIWVTNLNI
                     KPIGEGTLGGFAFAPPGLKNWPFGIGYPADNIDGVSIDYRFFGVNNYFAQSRPDIAEF
                     VGKGRTTVHEVGHYLGLRHIWGDYGNVFNLPCNNATHNLFFSDGMDDTPPCKAPFSST
                     IGEYRCDTTVNTCVIPYKGTDYPDLFENFMDYSGDACYNMFTKDQAAFLRFVLSTRRA
                     GIVSKREVETVSNIKIQNSTQNQFVLFPNPAKDYVQLQTTKKLDKDATVELFDISGQR
                     LKGFVFNKMDNNLIINTNELSPATYILKIYNSDWISTESFIKF"
     gene            10722..11405
                     /locus_tag="PLS16_07905"
     CDS             10722..11405
                     /locus_tag="PLS16_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNJ61042.1"
                     /translation="MKFTYYGHSCFLVEINNKRILFDPFITPNELAKDININDIKCDY
                     IAISHGHADHIADAVTIAKNNNSAIIVCAYEIYEWLSKQGLKNFRPMNTGGKWNAGNF
                     TIKCTAAQHSGGLPDGSYGGNPVGFIVSDGTQSFYYAGDTALTKDMELIPLWANLDFA
                     VLPIGDNFTMDVADAIIASDLVKTNKIIGVHYNTFPYIVINEKEAVEQFKAKGKELIL
                     IKIGESIEL"
     gene            11427..>11523
                     /locus_tag="PLS16_07910"
     CDS             11427..>11523
                     /locus_tag="PLS16_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ61043.1"
                     /translation="MGKIISIANQKGGVGKTTTAVNLAASLAVLEQ"
BASE COUNT         4168 a         1527 c         1823 g         4005 t
ORIGIN      
        1 aataaattct atacattcat gcgtaaatat tccctcttat ttattgttgt ttcgatttgt
       61 ttcaatgtat ttagtcaagc atctttaaag catacagata ttctgaaaat gtattatgaa
      121 gcaaaggagc aaattgctta tgaacgatac gcaatagctt acccaataat tcaagatttt
      181 ttgaaaaatt atgaaaataa tgtactaaaa attgacccat ctattgtttc tgaatcttac
      241 ttttataaag cactttgtgc taaagaaaca aaagcaccag aagctcaaac cttattaatt
      301 gattatattg aggattatcc tggaactaca catattaata ctgcaagatt tttactaggt
      361 gaaattgctt atgatatacc taattatcaa aatgccatcg agtattttaa agaagtaaat
      421 gaaaaggaat tgtccaaaaa tcaagcacta gattataatt ataaatatgc atttgcatta
      481 tttgctacta agaaatttaa agaagccaga gctcgtttta atacgcttac ggcttctgat
      541 tcaaaatata aagaagatgc tttttattat agtggtttgt cgtcttatta tttgggtgat
      601 tataataatg cttataagac ttttcaaaag attgaaactg ctaagaaata cgataaaatt
      661 gttccttatt atttagcttc tatccaattt gtcaataagg attacaaagg tgtggttgct
      721 tacgcagaac caaaactaaa ggagaatgta aaatatacta ctgaaattgc ccatttatta
      781 gggaattcat attacgaatt gcaacaatat gataaagcta aatactattt agaagaatat
      841 atgagcaaat ctgtaaaagc aacacctgaa gattattata ttttaggtta tgttcaagca
      901 caggaaaatg attgtaaaaa tgcagtaaaa aacttaggag aactttctcc attagaaact
      961 gaacttgcac aaaatgcgat gtatcaaatg gctaaatgtt tgataaaaat tgataagaaa
     1021 aatgatgcaa aaaatgcttt ccttcaatcg gcaagattag attttaataa agccattaaa
     1081 gaagagtcaa tatttcaata cgcaaaatta tcttatgagc tgaataatat taatgatgct
     1141 ttggttacac tcaaaaaatt tattgttgat tatccaaaat ctatctatta caacgaagca
     1201 aatgaacttt tagccgatat atttttaatt actaaaaact atgatgaagc gattgcaatc
     1261 attgagagtt tgcctaatgt ttctcctaaa ttgcaagcaa cttatcagca aatggcttat
     1321 ttaaaagcac tatctttgta taatgacaaa aattatgcac aggctattcc atttttcaat
     1381 aaatctttga aatatacaag caataaatct tatgaagcac taacttatta ttggaaagct
     1441 gatattgctc atcaaagtgg tgattataca caaagtaaac aattgttaga caagtttttt
     1501 agcttatcta aaaatatcaa tactgctgaa aatataatgg ttagtaatgg aacggcaaat
     1561 tatttacaag gctacaatta ttataaagaa aagaattatc ttcaagccgt agttaatttt
     1621 agtgctgctt taaaccagtt aaaagatagc aaaaccacaa gtattgcaca aaatatttat
     1681 cctgatgcgt tagtgcgttt agcagatgct tattttattc aaaagaaata tcaagatgca
     1741 aagcaaaatt acgatgtggt cattaagaat aattttaaag ctgcggatta cgctttattt
     1801 caaaatgcaa ttattgatgg tttagaagga aaatatttag ataaaattgc aggaatgaat
     1861 gcattagtag ctcgatttcc taaatcgtca tttgccgatg aagctttatt tcaagctgga
     1921 aatacgcagt ttgctattag taaaacaaat gatgcaaaac aaacttttaa tactttgctc
     1981 aaaaattatc ccaaatcgac aagagtgcca gacacttata ataaattagg tttaattgcg
     2041 tacaatcaag atagttatga tgaagcatta agttggtatc aaaaagtggt agataagtac
     2101 ccaaatacgc cagcagcaaa tgaagcgatg ttggttatta aagatattta tattgataaa
     2161 ggtgatgcta atgggtttat aaaatatgct tcaaaaaatt caagttctaa tttaactgct
     2221 tcagctcaag attctattat ttatcttaca gctgaaagtc aatttcaaaa aggaaatatc
     2281 aaacaagctt tagatggttt tgatagttat ttattacagt atcctaaagg ttttttcaat
     2341 ttgcaagcaa gattttatag agctgaatgt aattatagcc ttcaaagttt tgataaagca
     2401 ttgcaagatt acgtgtatgt agcaaattta ccacaaaata gattttcaga aaggtcggta
     2461 gctcgttctg caaatatttt ttattttgac caaaagaatt ataaagaagc tttaaactat
     2521 tattctaaat tagaacaaat agccacatta gatgaaaata aaagagaaag tttaattggc
     2581 ttaatgcgta ctaattttaa attaaataac catacttcaa cgctagttta tacagacaaa
     2641 gttagtgaac taagtaattt gcctgagttc tataaaagag aaattactta ttataaaggt
     2701 gtaacttatt ttaaacaaca agattacgat aaagcattac cgcaattgca aaccattgca
     2761 acgaccttag ataatgaaca aagtgcagaa gcccaatatg atattgctaa aatttatttt
     2821 gagaaaaaga attataaaca agcggagtca gaagccatga aatacacgga gaatttccct
     2881 gcttatgaat attatttagg aaaaacatac attttgttga gtgatattta taaagtaaat
     2941 ggcaaaattt tacaagctaa agctacttta caaagtttga tagataatta ttcttttgat
     3001 gatgatgtga tgaaagaagc caaagaaaag ttgcaaataa ttattgatca agaaacccaa
     3061 aattcaaatt taaaattgcc tgataataac ttacagatat tgcaatttga taaataatcc
     3121 tttcccttta ataaaatatt tatgagattt aataataata tatacctttt taaacaagct
     3181 ttcattctga ttagtatatc attttttagt tcttttgatg ctttagctca aaaagcaacg
     3241 gatacaatct atgatttaga tgtggtgaat attattcaaa gttatgagcc tattttaatt
     3301 aatagtgata aaattccttt attgcctgat gtgccaaagt tttctaaaaa taaaccagac
     3361 ccacaaactt atcaattttc tgatgtcaaa ggaaaaatta attattctgc tgaagatatt
     3421 agacctataa aaatgtcctt acctcaagcg gacaaacaaa aattcttata cttaaaagct
     3481 ggatttggga attatctaac accactttta aatctctcct tggtcaatcc aaaccaatcg
     3541 aagtttagag caggattaaa tacagatttt attttctcca aagcaaaaaa gccaagattt
     3601 caacagtatt ttgattttaa agccaaaggt tttggagaat attttattaa aagtagtgca
     3661 ttagttgggg cttctattaa tactcaattt gatcaatatc atttttatgc gaaccaagat
     3721 tctaatatta caagaaagga tatcgcaaat atgtataaac gttttggtgc aaatatctat
     3781 ttgaaaaata ttgcagataa agacttcgat tatcaagcaa atatcggatt taataacaca
     3841 agtaatactt tgaatcaaaa agaaacaacc attcaaattg atttgaaagg acattatatg
     3901 tttatgcaaa atattggtgc tggagcgaat atattagtca ataatgttgg ctataaagat
     3961 gctatagact ccaacaaaac caatcgtttt tctatgggta ttaatcctta tgcaattgtt
     4021 caatataaaa tttggaagtt gaaattgggt gcaaatgtta tgggtacaaa taagaaatta
     4081 tatgttttgc cttctatcca aaatgttttg aatatttata agaattattt gactatgtat
     4141 aacgaatgga actcacaggt aaaagtgaat agtttacata aaaattcatt agaaaatgca
     4201 ttcatagatg ccaataattt ccgaaatcaa attgatgaaa aaagaacttt tataggttta
     4261 agaggtgcag caagtggctt ctattatgac ttaaatttct ctcaattggt ttccagaaat
     4321 gccttattgt acgcttataa cgaatatgat aatacatatg acgcagctat atctccaaga
     4381 attggtacgt ttcagcctga gtatattcac aaaattaaag cactcaatcc gcatattgct
     4441 ttaggttatg ccaaaggaaa caaatatgga gcaaaactat catttgatta ttatatatac
     4501 aataaaaaca atgattttga agtgatatat aagcccaaac ttcaatctaa attatcagta
     4561 ttctacaatt ggaacgaaaa attatttatt gatttgagta tgacagctta tggaaaaatg
     4621 aatggtttag tgcatcaagt ttcaaacgat gtaattgtaa atacttattt gcaaccaatt
     4681 aaaggtgttg ttgatattaa tttgagtgca tcttactttt ttacaaaaaa tattggtgtg
     4741 tttttggatt taactaacat ttcgcatcag aaatatcaac attatatcaa ttatccaagt
     4801 tatggtgtac aagtaattgg tggtgtcaaa attgcttatt agatttgtga tagatgaatg
     4861 catttatcac acagctttta tatcaaaata attgtgtaat cgttcctaaa gttggagcgt
     4921 tcttagccaa ttataagagt gcctttgtgg atatgcacca aaatattatt cttccacctc
     4981 aaaaaacaat tgcttttaat aagcaattga ataaaagtga tggactactt gcggcattcg
     5041 ttagtgaaca aaaaatgatt tcatatacag ctgctgaaca attggtgaat caatttggtg
     5101 atgagatatt gttttcatta gaaaaacata aaagttttat ttttagtgga attggtcgat
     5161 tatataaaga tgcttcagat aatattttgt ttgaaagctt tgggcaagaa aattttttaa
     5221 tagagaatta tggcttgttg cctgttgcaa ttgaaccaat ttcaagattg aaatctgaag
     5281 cagttattga gccaattgta ccaacagttg cagttgatga aaaacaagtt agtgtctcaa
     5341 ataaaaagag tggaattcgt tgggtgttac ctgtaattat tccattttta ttgataacta
     5401 tcattggatt ttttgtgatg ttttcacaat ttaaccctca gttttctaag ttcgatttag
     5461 cttcactagt tccaaatgtt aagcaacttt ttgagcaaaa aaatcaacaa aatattctta
     5521 aaactgaata tccaaaagca actattccta cacaaaaaga tacaactcat ttagtggtta
     5581 aagcaaatat tgattctgtt ctaattaata cttcaacatc agttgctgtt gatacaatta
     5641 cgccaataaa aaaggaagtt gtacaaactg aaaagccagt tgaagtaaaa gaacctgaaa
     5701 agaaacctat tgttttagag aacggaaaag tggctaatgc atttatttgt gtaggttctt
     5761 ttaagaatga aagtaatgct aaagaaatgc aaaagatgat tgatttttta ggattgcaat
     5821 ctgtagtaag caaaactaaa ttacatcaag tattggtggc aactagtaca gcaagagctg
     5881 gagaagattt agcaacactt aaagaagaaa ttgcaccagg tgcatatatt tactgccaaa
     5941 attgtaatta ttaattattt tgattagttg ctcagatata cataaatctt atggtcaatt
     6001 agaagttctg aaaggcatta atcttcaagt taatcaaggc gaattagtta gtgttgttgg
     6061 tgcatcaggt gctggtaaaa gtactttgtt gcatatcatt ggtacattag ataagccaga
     6121 tagaggagag gtaatcatca acggaatgtc tgttgcaaac ttgaaagaca aagccttgtc
     6181 tgagtttaga aataaaaact tagggtttat ttttcaattt catcatcttt tgcccgaatt
     6241 tactgcttta gaaaacgttt gcatacctgg atatattgcc aatagaaaag atgcagaagt
     6301 gcaaaaccga gcgaaagaat tgttggattt tatgggttta aaagatagat ttcatcataa
     6361 accaaatgaa ttgtctggcg gagagcagca aagagtttca gttgctagag ctttaatcaa
     6421 taatccactt ttgattttgg ccgatgagcc tactggaaat ttagattctg aaaaatctga
     6481 agaattacac aacttgtttt ttgatatgaa gcggatgttt aaccaaacat ttattattgt
     6541 tacacataat gaacatttgg ctgaaaaagc agataagaaa atcgtaatga aagacggtct
     6601 tataatttct taattactca actttgtaac ttgctacaca atcaatgacc gcattaaatt
     6661 gcttgtttaa tgtaaattgc tttccaattt gtgtatcata aactggaatt tttaattcgc
     6721 ctataaaatc attaaaaaca atatcttcat cttcaactaa aaatctaatt tcatcatctg
     6781 tgcttaccaa aaattgaata tttttagatt ttggtccttt agcaaaaata taatcatctc
     6841 taacagcttc tttccaaatt atagtatttg ccaacataat ttttacggat acatctggag
     6901 ctaaagtatt gtaaaaccca gcatcccaag tttgtccatt tgacatcaat ttattgactt
     6961 ctaagtaatc aatattgaaa gttattttat gtgtaggctt tttctttagc acaatatttt
     7021 tctcaattat tacttgttga tatttattgc ccaatgcatc agttccattc agtccaacca
     7081 aaacattgat ggtgtgttct ccttcgctta aattaaaagc tgctagcgga ataaataatg
     7141 attctttaat tggataatta ctgccaaaat caaaggagtc atactttttt ataagtgtaa
     7201 gatttccatt tttatttttc aatttttgat aacctaaagc aggctttatt tttgaaccat
     7261 tttctgagaa ggttatattt aaagtaaatt tacttaaatc cattatggaa tcattagaaa
     7321 ttttatcttc taatgctttg aaatcaattt cataattaaa atcaatgcca taaactcctt
     7381 tattgttgat gttaggtagt gttttggcat tgcgaataaa aattatagat tcattattgg
     7441 cagcatttaa tgcaaaaaat gtaaagaata caaaaagaaa taataagttt ttcatggttt
     7501 gaatttgttt ctgtaaatct ataaaaaata agtttatagc agtatagatt aacaattctt
     7561 gagtaaaaaa taggttttgt ttaattttaa ataactaaaa aattataggt tttatttttt
     7621 tgcaaagttt aattttttca gaagacatcg ccataaattc tttttttttc ctatcttgct
     7681 gatgtattat cggtactgaa atggaacaac aagaacaaca agcagctcaa caaaaaaaga
     7741 taaagaaaga gtattttgcc gtagtagatg gattgcgtgg tgcatctatg tgttttacag
     7801 ttttggtaca catgaatttg acaattttta agttgccagc atttttagca cattttggtt
     7861 tacatggttt ttatgtactg tctgcatttt taatcagtac catattgtac aaagagaaag
     7921 taaaatttgg cgaatttaaa ccttatgcta agaattttta tatcaaacga tttctaagaa
     7981 ttattccatt atattatgcc tatattatag gtatcgcttt tatcggctta atattaaaat
     8041 ttattacaca tggcgaccca tttggtatta tttatgagct taaaaatttt ggaataaatt
     8101 tattcacttt tacctataat tataaagatt tatttgcatt cttaaaaggc ttagaccatc
     8161 aaaagtgtaa cttatttcct catttatggt ctatctcaat tgaagaacaa ttttatttag
     8221 tgattcctgc tgttttattt ttcttgacta aagaacaagt gaaaaaaatg gcaatagcaa
     8281 taattatcat atacccattt tttagagtag caggatttct atatttaaga gatgtattgc
     8341 acatgacatc aacacatggt aaacatgagt atgaaattat gtacaatttc ttctatcgtt
     8401 ctagtttatt tcaagtagat gcttttatgt atggtttatt aattcctttg gtaaattata
     8461 ataatagaaa agtattacgt tggattgtaa ttggctcaac ggcattgata tttgtaggtc
     8521 agatttataa tatgtttgat tatgctcatg aacaaggtgt atctgcttgg agtgtagtag
     8581 gagagcatgt cgttgtgatg aaaaatggta tgtatgctta tgtgaataca ttgtataata
     8641 ttttggcatc ttcattaata tattatttat taaaatttcc tgattcatca ttaaataaaa
     8701 tattgaaatt cccatttttt atggaatggg ggaaaattgt atatggtatc tatgtttatc
     8761 acatgatatt tgtaaccatt acagctatca ttttttatac agtgcttaaa gtctatttac
     8821 cattaccgat tgcagagtta ttgtatatag cattatgttt tacttctgtt tattatttct
     8881 ccaaattcag tttctataaa tttgaaatgt atttcttaaa tatcaagaat aagagagatt
     8941 aaaaaatatg attgatgtaa gtaattttat tgtataatgt aaataatttc aacgaaaaag
     9001 taatacattt atttaatgaa aaatatcaat tacataaaaa tccttttggt tctattaaca
     9061 gtatcaattt ttagtagaat taatgcacaa catatttgtg gacaagatat tattacacat
     9121 caactccgct cattatcgtc agattatgat aatgactatt ctaatttatt agaagaatgg
     9181 cagtatgaaa agcagaatgc aagaacttct gctttggaag ataccaccta tacgatacaa
     9241 gtagtggtgc atgttgttta tttagaagat aatttaattc aaaatattcc agatagcatc
     9301 attcattcac aaattgatgc attgaataga gattttggtg ttactaatga agatactgta
     9361 aaccttagac caatttttaa accattcaga ggatatccaa aaataaaatt tgaattagct
     9421 aaattagata catcaggaaa tcctactact ggaattattc gtgttgcagg taaggcacct
     9481 tctgcaaatg cagtttttac aaatcctttg gattctattc taagtaatct attaaatgca
     9541 gttggtgatt ggtttaaaaa aggttataca atttctactt tggctggaaa tgtaaaggat
     9601 acaaccatcg gttctccaat ttgggacaat aaaaaatatc tgaatatttg ggttacaaac
     9661 ttaaatatta aacctattgg tgaaggtact ttgggtggtt ttgcatttgc acctccaggt
     9721 ttaaaaaatt ggccttttgg aattggttat cctgccgata atattgatgg cgtatctatt
     9781 gattacagat tttttggtgt aaataattat tttgctcaat ctagaccaga tattgcggag
     9841 tttgttggaa aaggaagaac cacagtccac gaagttggac attatctggg tttaagacat
     9901 atttggggag attatggaaa tgtctttaat ttaccttgta ataatgccac acataattta
     9961 tttttctctg atggtatgga cgatactccg ccctgtaaag caccattttc atctacaatt
    10021 ggtgaatata gatgtgatac aaccgtgaat acttgtgtca ttccatataa aggcacagat
    10081 tatccggatt tatttgagaa ttttatggat tattctggtg atgcttgtta caacatgttt
    10141 actaaagacc aagctgcttt tttacgtttt gtattatcta caagaagagc tggaatagtt
    10201 tctaaaagag aagttgaaac tgttagtaat atcaaaatcc aaaatagtac acaaaatcaa
    10261 tttgttttat ttccaaatcc tgctaaagat tacgtacaac tacaaacaac taaaaagtta
    10321 gataaagatg ctactgttga attgtttgac atatctggtc aaaggttaaa aggttttgta
    10381 tttaataaaa tggataataa tttgattatc aatacaaatg aattgtcgcc agctacttat
    10441 attttgaaaa tttataatag tgattggata agcaccgaaa gttttattaa attctaagtt
    10501 tctatttttg ttaaaaagta gagctttatt ttgtgctata tttttattcg ataagtttag
    10561 gtattaataa ttaatcactt aggtatatac acattttatc cacattgaat tgtgtgtaag
    10621 tattggaaaa cttaggttta acttacccaa cttatatact aatttagtag cgtggaacat
    10681 tcgtgtaaca tttttaaatt gcttagtaaa agaaagaaaa tatgaaattt acgtattatg
    10741 gtcattcttg ttttttagtg gagattaaca acaaacgtat tctttttgat ccatttatta
    10801 caccaaatga attagcaaaa gacattaata taaatgatat taaatgcgat tatatcgcta
    10861 tttcacatgg acatgctgat catattgcag atgctgttac aattgctaaa aataataact
    10921 cggcaattat agtttgtgct tatgagattt atgaatggtt gtcaaaacaa ggactgaaga
    10981 atttcagacc aatgaataca ggtggaaaat ggaatgctgg aaattttacg attaaatgta
    11041 cagccgcaca acattcaggc ggattacccg acggttctta tggcggtaat ccagttggtt
    11101 ttattgtgag cgatggcact caaagttttt attatgctgg agacactgca ttgacaaaag
    11161 atatggagtt aattccactt tgggcaaatt tggattttgc agttttacct atcggtgata
    11221 attttacgat ggatgtagcc gatgccatta ttgcatcaga tttagttaag acaaataaaa
    11281 tcattggtgt gcattacaat acatttccat atattgtcat taatgaaaag gaagctgttg
    11341 agcaatttaa agcaaaagga aaagaactta ttttaataaa aataggagaa tcaatagaat
    11401 tataaaatat agattataga aatacaatgg gaaaaattat tagtatagca aatcaaaaag
    11461 gtggtgttgg taaaacaaca acggctgtaa atttagcagc gagtttggct gttttagagc
    11521 aaa
//
LOCUS       DAOQCW010000078        11463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_2121_length_11463_cov_5.263587, whole genome shotgun sequence.
ACCESSION   DAOQCW010000078 DAOQCW010000000
VERSION     DAOQCW010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11463
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2121_length_11463_cov_5.263587"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..370
                     /locus_tag="PLS16_07915"
     CDS             116..370
                     /locus_tag="PLS16_07915"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNJ61044.1"
                     /translation="MLIASVSDFRKDIKSYLDRVTKNFETLIINRGKENGIVVMSLEE
                     YNSLMATNHELSSRKNEMRLDSAIKKLKNGKTFQQQLIQD"
     gene            372..629
                     /locus_tag="PLS16_07920"
     CDS             372..629
                     /locus_tag="PLS16_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HNJ61045.1"
                     /translation="MKYIFVDESWDDYLYWQKTDKKKLKRINELLKDISRSPIDGIGK
                     PEPLKHKYSGFWSRRIDNEHRLIYKYEEGQILIAKCRFHYD"
     gene            1003..1914
                     /locus_tag="PLS16_07925"
     CDS             1003..1914
                     /locus_tag="PLS16_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNJ61046.1"
                     /translation="MSKISLLILAAGMGSRYKGQKQIDTITDYNESLLEFSLYDALQV
                     GIRKFVFIINNQFPEDFQQKLNQIAKNNSFEIHFVIQTIDKYIPLSFLPKLNGRMKPL
                     GTAHAILCANEIIQEVFITINADDFYGRQSYTEAINMINQQQIDENNYGMIAFKLINT
                     LSENGAVSRGVCSIRDQKLISIEEFTNIYKKALVIQGKDENLNTQTLHEADLVSMNFW
                     ILHPSFFKFAQYNLLKFLNAHEDLSTIEFYLPSVINYAIHHHVNLRVSQSSEKWFGLT
                     YREDKTIVTNEISKRKGLKEYPNKLWH"
     gene            1940..2974
                     /locus_tag="PLS16_07930"
     CDS             1940..2974
                     /locus_tag="PLS16_07930"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HNJ61047.1"
                     /translation="MNTIEVTSIFKNFEAIAPDFTVVHLTSGHINHTFLVQNNQKQYI
                     LQKVNTSIFKNLAIITLNIKIISKHLQSKLYPYKILTPLVFQSGDYLHDKSWRIFEYL
                     KDTQIFEKVQSKEQAYEAAKFLSLFHFYLKDIDLNNIQDSIDGFTNFNSRILHFEIAL
                     QNALPERLLTAKAEIEAIHHQYNIFEQWNNIVKRMPKRLIHADPKISNFLFHKNSSSE
                     IKALIDWDTFMCGTILYDFGDMVRSYTNLRAEDDAENYNNFSFENYLALKEGFLFYLK
                     DELEPIEIENFELIAQMVILIQAIRFLTDYLNHDKYYIIHYPEQNLNRTRNQLNLLHE
                     MNHILSKNKV"
     gene            complement(3084..3503)
                     /locus_tag="PLS16_07935"
     CDS             complement(3084..3503)
                     /locus_tag="PLS16_07935"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNJ61048.1"
                     /translation="MATNRTFTMIKPDAVEANNIGSILAMITEAGFKIVAMKYTKLSA
                     EQAGQFYEVHKERPFYGELVEFMSRGPIVAAILEKDNAVETFRNLIGATNPAQAEEGT
                     IRKRFAKSIGENAVHGSDSDENAAIEGNFFFSNLERF"
     gene            complement(3627..4031)
                     /locus_tag="PLS16_07940"
     CDS             complement(3627..4031)
                     /locus_tag="PLS16_07940"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNJ61049.1"
                     /translation="MFEETIQLRVRYHDCDPMGIVYHGHYAKLFEIGRTEAMRKHGFS
                     YQILEQQGFAMPVVEMNIKYLRPARYDELIDIKTIIEEMPNRFLIVKGIIFNQDKKVL
                     TESIFKFVFVKRETLRRCSPPVFILEQIKPYF"
     gene            complement(4031..5044)
                     /gene="mltG"
                     /locus_tag="PLS16_07945"
     CDS             complement(4031..5044)
                     /gene="mltG"
                     /locus_tag="PLS16_07945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNJ61050.1"
                     /translation="MNLKKILSSFVIIGVIALLLVYLLFFKSNIKNEYTLYIPTGSTF
                     NDVLKQLDRNKVLKNKATFVELAKKIGYAEKVKPGRYIIHKRYSNIELLRKLRNGNQD
                     AVKIVLNNIATLEQLVDLLSDKLEPNKKLFHQAFTDKHLLDSLGLNEENVLTLFIANT
                     YEFYWNISAEAFTYKMLKEYNKFWNDNRLRSAENLGLSPTEVTILASIVQKESTKYDE
                     YDRIAGVYYNRLLKKMKLQADPTVLYAKQRLGKANRVYNKDTKIAHPYNTYYIDGLPP
                     GPICMPEMKSIDLCLDLEHHDYIYFCAKEDFSGYHSFAKDWATHKINAKKFHDAMNAK
                     NIR"
     gene            complement(5127..5915)
                     /locus_tag="PLS16_07950"
     CDS             complement(5127..5915)
                     /locus_tag="PLS16_07950"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNJ61051.1"
                     /translation="MMNTFFKYILFFLFLTNVAFAQVNYEDDKNIPERPDPPKLVNDF
                     AHLFPDYNKFQLENKLVAYNDSTSTQIVVVTVENLNGYAEDDYSLRLGRKWGVGQKGK
                     NNGIIILISKEERAVNIELGYGIESYISDRDAKQMIEYIMIPRFREGDYFGGVDSTVN
                     LMISMLQGTYKPTEIVQEDNNAFWVFVVFVGLFAIIFLIAYFAPPSNGGGSGGSYTGG
                     SGGWRSTPSWGSGGSSWSGGGGSSGGFGGFGGGSFGGGGASGRW"
     gene            complement(5919..6371)
                     /locus_tag="PLS16_07955"
     CDS             complement(5919..6371)
                     /locus_tag="PLS16_07955"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNJ61052.1"
                     /translation="MGKSIEKFFSEKEKEFITDAIRNAEEQTTGEIRIRIESSTNSTD
                     VFECARKDFEELGMHKTKHRNGVLLYIAINDKQFAIIGDIKIDAIVGQKYWHNLSVTL
                     GEYFSRKQFAEGTITIINRIGEILHQYFPIKDDEENNDELPNEISFGD"
     gene            complement(6398..6976)
                     /locus_tag="PLS16_07960"
     CDS             complement(6398..6976)
                     /locus_tag="PLS16_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNJ61053.1"
                     /translation="MKRILKFVIVLMVMPMLSSCSYNTMISLDEQVKEAWANVEAAYQ
                     RRTDLIGNLVETVKGSGKFEKEVLTDVINARSKASSMQVNANDLTPENIAKFQEAQSQ
                     LGGALSRLLVTVEKYPDLKTTAQYAELMNELNRTENRINVARVDFNNAVKSYNTTIRS
                     FPNNLVAMIFGFKAKDMFKAEAGAEKAPKVSF"
     gene            complement(7011..8501)
                     /locus_tag="PLS16_07965"
     CDS             complement(7011..8501)
                     /locus_tag="PLS16_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNJ61054.1"
                     /translation="MSQLFAKKSVQQLLKESENSGEDSLKRTLSSLALISLGIGVVIG
                     AGIFVRTAAAAAQNAGPSVTLGFVVAGIGCALAGLCYAELSSSIPISGSAYTYTYATM
                     GEFLAWIIGWDLVLEYAVGAATVGIAWSEYLNDFLTQILGVAPIPYQWCHSPFQVSVD
                     GVQGIVNLPALGIVTAVSFLLIKGIQESAFVNNIIVIVKVAIVLLIITLGWSYINPAN
                     HTPYIPEPTKYTDHDGITYNFGGIMGILGAAGTVFFAYIGFDAITTAAQETKNPKKAM
                     PVGIIGTLIICTILYILFSYVLTGLESVEFFRDPNKGGEASVSAAIQSAMPAYTWLSK
                     SITIAILAGFSSVILVLLLGQSRVFYSMSRDGLLPKAFSEIHPKYKTPYKGTLAVLVL
                     VALFAGFVPGNVVGHMTSIGTLFAFILVCVAVIVLRKVEPDMPRQFKTPFVPVVPILG
                     ILFCGAMIFGLGVENWMRLIVWLLIGFVIYFGYSKKHSKLNNSNNK"
     gene            complement(8587..10113)
                     /locus_tag="PLS16_07970"
     CDS             complement(8587..10113)
                     /locus_tag="PLS16_07970"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNJ61055.1"
                     /translation="MKSVEIEDVFVRTKQLYPYQEAYIKNIYEALVENESRDNIVFQL
                     PTGGGKTIIFSEIARRYIEHQEKKILILTHRIELLKQTSVALEEAAVKCKLITSETED
                     IYDQKDYNCFLAMVETLNNRLKEDDEFLKGIDLVIVDEAHYNSFRKIFKYFEHAVILG
                     VTATPLSSNIHYPLRSNYRKLIVGDSIKDLIHNGFLSNAKTFTFDVKLGGLKVGIDGD
                     YTVNSLDRIYMGFDMHQILLSAYKEKAKGTKTLIFNSSIATSKSVENFFKIHNIPIRH
                     LDSTSNKQERKDVLKWLKETPDAIVTSVGILTTGFDEPTVETIILNRATRSLTLYHQM
                     VGRGSRRLPNKSEFSIIDLGNNAKRLGIWQDHIDWQDVFVNPDRFLEHLYDREQKMER
                     GLMFTLSDEVMARFPNTQNFDFDAEKEYQKLYLKGLKTLAVLDLAVDMHYERIKENCT
                     NYLDAVELINIIQDEIQYILNVYISCLSKATKNYFNFLLEEYNQKLHKKLKDNLPFSE
                     "
     gene            complement(10165..11088)
                     /locus_tag="PLS16_07975"
     CDS             complement(10165..11088)
                     /locus_tag="PLS16_07975"
                     /inference="COORDINATES: protein motif:HMM:NF040656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYDIA family GHMP kinase"
                     /protein_id="HNJ61056.1"
                     /translation="MLVDASYTANGKLLLTAEYFILDGAKGLALPTKYHQKLVLQTIQ
                     SEILEWKSYDENGEIWFYCNFDKHLNLIDYSDIEVSEMLRKILKTASILKKNNSVHGV
                     KSSTYLNFNRHWGLGTSSTLISLVAQWFDINPYQLLEQTFGGSGYDIACATAYKPILF
                     SKNECNEIKVELCNFNPPFKNQLYFVYLNQKQNSRTAIQYYKQTKIEDRQAIIQQLNE
                     LTKIISNTTDKFEFDKCLIQHENIISRFLSLEKVQDKYFSDFNGVVKSLGAWGGDFVL
                     VSNDLDMDYVKKYFHSKGYNNIISFNDMIQD"
     gene            complement(11117..>11463)
                     /locus_tag="PLS16_07980"
     CDS             complement(11117..>11463)
                     /locus_tag="PLS16_07980"
                     /inference="COORDINATES: protein motif:HMM:NF028180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HNJ61057.1"
                     /translation="KVKRVLLCSGKVYYDLLERKESTDRKDIAIVRVEQIYPLAKKQY
                     QAILDKYKNAEFVWVQEEPKNMGAWTFVLSNYPNGKDLKLISRAQSASTATGFSKIHA
                     QEQKAIVDEAFA"
BASE COUNT         3972 a         1967 c         1506 g         4018 t
ORIGIN      
        1 ggttctcgca ttaacagtag tgctaaaatg ccctgcctgc gtcaagccgc aaaaacgtta
       61 aatttggata gtataaaatt tgtatgtata tttgtacgaa ttaaagtaca aaattatgtt
      121 aattgctagt gtttctgatt tcagaaaaga tataaaatca tatttagaca gagttacaaa
      181 aaactttgaa actctgatta taaatcgtgg aaaagaaaat ggaattgttg taatgtcgct
      241 tgaagaatat aattctttaa tggcaacaaa tcacgaactt tcttcacgca aaaatgaaat
      301 gcgtcttgat tctgctatca aaaaattgaa aaacggaaaa acttttcagc aacaactaat
      361 ccaagattga gatgaaatat attttcgtag atgaatcttg ggatgattat ttatattggc
      421 aaaaaacaga caaaaagaag cttaaaagaa ttaatgaatt attgaaggat atttcaagaa
      481 gtccgattga tggaattgga aaaccagaac cattgaaaca taaatattcc ggattttggt
      541 cacgccgaat tgacaatgaa cacagattaa tttataaata cgaagaaggg caaatcttga
      601 ttgcaaaatg tcgatttcat tatgattaat tatacaaaaa attacttctg ctaacataat
      661 tacaaatagt gtaaagccct gagcgttggc tcaggacttt ttgtctcgct ttaagcaaga
      721 tgttcatcga cacgattgta atttttctca taggtggttt ttagctttta tccactctat
      781 tttataaaat ttggagtgtc gaaaactgtt gtaggacaaa acagaagctc ctactaaatt
      841 taggcaaaag gtaattttac cttttaagga aaataaaagt atatttagaa cctttaaaat
      901 ttgaagctgg tttccaaagt tgttttattt aattcttaat acttgtatta gtgtttttcc
      961 agcataatta gtactacttt tgtcttataa attaaacatt ttatgagtaa aatttcgcta
     1021 ttaattttag ccgctggaat gggaagtcga tacaaagggc agaagcaaat tgatacaata
     1081 actgattaca atgaaagttt attagaattt tctttatatg atgctttgca agtgggtatc
     1141 agaaagtttg tctttataat taataatcaa ttccctgaag atttccaaca aaagctcaac
     1201 caaattgcga aaaacaattc gtttgaaatc cattttgtca ttcaaaccat agataaatat
     1261 atacctcttt catttttacc taaactaaat ggcagaatga agcccttagg tactgctcat
     1321 gctattttat gtgctaatga aatcattcaa gaagtattta ttacgataaa tgctgatgat
     1381 ttttatggaa gacaaagtta tacggaagca ataaatatga ttaaccagca acagattgac
     1441 gaaaacaatt atggtatgat agcttttaaa ctaattaata cattaagtga gaatggagca
     1501 gtttcaagag gtgtatgtag tatcagagac caaaaactta taagcattga agagtttact
     1561 aatatttata aaaaagctct agttattcaa ggaaaagacg aaaatctaaa cacacaaaca
     1621 cttcatgaag ccgacttagt atctatgaat ttttggatac ttcatccttc ttttttcaaa
     1681 tttgctcaat ataatttatt gaaatttcta aatgcacatg aagatttatc tacaatagaa
     1741 ttttatttac catctgtaat caattatgcg attcatcatc atgttaattt aagggtaagt
     1801 caatcgtcag aaaaatggtt tggacttaca tatagagaag acaaaactat agttaccaat
     1861 gaaatctcta aaagaaaggg acttaaagaa tatcctaata aattgtggca ttaattaatt
     1921 ttctcaaata acttccaaaa tgaatacaat agaagtaaca tcaattttta aaaattttga
     1981 agcaattgca cctgatttta ctgttgttca tttaacttct ggacatatca atcatacttt
     2041 tttagttcaa aataatcaaa aacaatatat ccttcaaaag gttaatactt ccatttttaa
     2101 aaacttagca atcataacac tgaacattaa aattatttct aaacatctcc aatcaaagct
     2161 atatccctac aaaatattaa caccactagt ttttcagtct ggcgattatt tacatgacaa
     2221 gagttggcga atttttgaat atctgaaaga tacacaaatt ttcgaaaaag ttcaaagcaa
     2281 agaacaggcc tatgaagctg caaaatttct aagtttattt catttttatt taaaagatat
     2341 agatttaaat aatatccaag attccataga tggctttact aattttaata gtagaatcct
     2401 tcattttgaa attgccttac aaaatgcttt acctgagcgt ttactcactg caaaggctga
     2461 aattgaagcc atacaccatc aatataatat ttttgaacaa tggaataata ttgtcaaaag
     2521 aatgccaaaa aggttaattc atgcagatcc aaagattagc aactttttat ttcataaaaa
     2581 ttcttcatct gaaatcaaag cactcataga ctgggatact ttcatgtgtg gcactatact
     2641 ttatgacttc ggagatatgg ttaggtctta taccaattta agagcagaag acgacgctga
     2701 aaactataat aatttcagtt ttgagaacta tctagcttta aaagaaggat ttttatttta
     2761 cttaaaagac gaactggagc ctatcgaaat agaaaatttt gaactaattg cacaaatggt
     2821 tattctaatc caagccattc gtttccttac agactattta aatcatgata aatattatat
     2881 cattcattat cctgaacaaa atctaaatcg aacacgaaac caacttaatc tattacacga
     2941 aatgaaccat attctttcta aaaataaggt gtaaaaaggt aatatctgaa aaatatttgg
     3001 tgggtaaata aaagaggctg tccgaaattt tggacagcct cttttgtatt taaaatgatg
     3061 ttgcagagca acaaaatttc ctattagaat ctttctaagt tagaaaagaa gaaatttcct
     3121 tcaattgctg cattttcatc ggaatcagaa ccgtgaacgg cattctctcc tattgatttt
     3181 gcaaatcttt ttctaattgt accttcttca gcttgtgctg gatttgttgc accaattaaa
     3241 tttctaaaag tctcaacagc attatctttt tctaagatag cagcaacaat tggacctcta
     3301 ctcataaatt ctactaattc accatagaaa ggtctttctt tgtgaacttc ataaaattgt
     3361 cctgcttgtt cagctgataa tttagtgtat ttcattgcta caattttaaa accagcctct
     3421 gtaatcatag ctaagataga accgatgtta tttgcttcaa cagcatctgg ttttatcatg
     3481 gtaaatgttc tgttcgttgc catattattc taatttttgt gcaaaaataa actttcaatt
     3541 gttcttaata aacaataact tattaacatt tgattacgaa taaattgtaa ggatgcaatt
     3601 agttcaactt ttaaaacatc tgttttctaa aagtaaggtt taatttgttc taaaataaat
     3661 actggtggag aacatcttct taaagtttct ctttttacaa aaacaaattt aaaaatagat
     3721 tctgttagca cttttttatc ttgattaaag ataattcctt ttacaattag aaagcgattg
     3781 ggcatttctt caatgatggt tttaatatca attagttcat cgtatctagc aggtcttagg
     3841 tatttgatat tcatttctac aacaggcata gcaaaccctt gttgttccaa tatttgataa
     3901 gaaaagccat gctttcgcat tgcttcagtt ctaccaattt caaataattt agcataatgt
     3961 ccatgatata ctatacccat tgggtcacaa tcatgatatc ttactctaag ttgtatcgtt
     4021 tcttcaaaca ttatcttata tttttagcat tcattgcatc gtggaatttc ttagcattta
     4081 ttttatgagt tgcccaatct ttagcaaaac tatgataacc tgaaaaatct tcttttgcac
     4141 agaaatagat gtaatcatga tgttctaaat ctaaacataa gtcaatactt ttcatttcag
     4201 gcatacaaat tggccccggt ggtaaaccat caatataata agtattataa ggatgtgcaa
     4261 ttttggtatc tttattatat acccgattgg cttttcctaa acgttgcttc gcatataata
     4321 ctgttgggtc tgcttgcaat ttcattttct ttagcaatcg attataataa actccagcaa
     4381 ttctatcata ttcatcatac tttgtagatt ctttttgaac aatagaagcc agaatagtaa
     4441 cttcagttgg cgacaatcct agattttcag cactccttaa cctattgtca ttccagaatt
     4501 tgttgtattc ttttagcatt ttataagtaa atgcttctgc agagatattc caataaaact
     4561 cataagtatt ggcaataaac aatgtcaata cattttcctc attcaaacct aaagagtcta
     4621 acaaatgctt gtctgtaaaa gcttgatgaa aaagtttttt attaggctct aatttatctg
     4681 ataataaatc aactaattgc tctaaagttg cgatattatt caatacaatc ttaacagcat
     4741 cttgatttcc atttcttaat tttctaagta attcaatatt actatatcgc ttatgaataa
     4801 tatatctacc aggttttact ttttcagcat aaccaatttt cttagctaat tcaacaaatg
     4861 tagccttatt tttaagtact ttattcctat ctaattgttt taatacatca ttaaaagttg
     4921 aacctgtcgg aatatataag gtgtattcat ttttgatatt acttttaaag aataataaat
     4981 aaactaataa aagtgcaatt acaccaataa taacgaagga cgacaatatc tttttaagat
     5041 tcataatgaa tgactactaa tttacaaatc tatacagata ttgtgtgtta ttataataaa
     5101 aaaactttgc ttacaatcca aagaaactac catctaccac ttgcaccgcc gcctccaaag
     5161 gagccacctc caaagcctcc aaaaccacct gaacttcctc ctcctcctga ccaacttgac
     5221 cctccagaac cccaactagg cgtacttctc caaccgccac ttccgccagt atatgaacct
     5281 ccacttccgc cgccattgct tggtggagca aaatatgcta ttaaaaatat tatcgcaaac
     5341 aatccaacaa agacaacaaa cacccaaaag gcattgttat cctcttgaac aatttcggtt
     5401 ggtttataag ttccttgaag catagaaatc ataagattaa cagtcgaatc tacgcctcca
     5461 aaataatcgc cttcacgaaa tcttggaatc ataatatatt ctatcatttg tttggcatct
     5521 ctatccgaaa tataactttc aataccatag cctaattcaa tatttacggc acgctcttct
     5581 ttggaaatta gtatgattat cccattattt ttgccttttt gaccaacacc ccattttcta
     5641 cctaatctca aagaataatc atcttcagca tagccattta agttctctac agttaccacc
     5701 acaatttgtg ttgaagtaga atcattatag gctactaatt tattttctaa ttgaaattta
     5761 ttgtaatcag gaaataaatg agcaaaatcg tttactaatt taggtggatc aggtctttca
     5821 ggaatatttt tatcatcttc atagtttact tgtgcaaatg caacatttgt gagaaaaagg
     5881 aaaaataata tatacttaaa aaaagtgttc atcatcaatt aatctccaaa tgaaatttcg
     5941 ttaggtaatt catcattatt ttcttcatca tctttaatgg gaaaatattg atgtagtatc
     6001 tctccaatgc gattaataat agtaatcgta ccttctgcaa attgttttcg agaaaaatat
     6061 tctcctaagg taacagataa attatgccaa tatttttgac ccacaatagc atctatttta
     6121 atatctccaa ttattgcaaa ctgtttatca tttatagcaa tatataacaa aacaccatta
     6181 cgatgtttgg ttttatgcat tcctaattct tcaaaatctt ttcttgcaca ttcaaataca
     6241 tcagtactat ttgtactaga ttctattcta attcttattt ctccagtggt ttgttcctca
     6301 gcattacgaa tcgcatctgt gatgaattcc ttttcctttt cactaaaaaa tttttcaata
     6361 gactttccca tactgataat ctaaattatc aaaaaaatta gaaagaaact tttggtgctt
     6421 tttcagctcc tgcttcagct ttaaacatat ctttagcttt gaaaccaaaa atcattgcaa
     6481 ctaaattatt aggaaaactt cttatcgttg tattataaga ttttacggca ttattaaaat
     6541 caactctagc aacattaatt ctattttctg ttctattgag ttcattcatt aattcagcat
     6601 attgagctgt tgtttttaaa tcagggtatt tttctacagt tactaataat cttgataatg
     6661 caccacctaa ttgagattgt gcttcttgaa atttagcaat attttcaggt gttaaatcat
     6721 tcgcatttac ctgcatagaa cttgctttag atctagcgtt gattacatca gttaaaactt
     6781 ctttttcaaa ctttccactt ccctttacag tctctactaa atttccaata agatctgttc
     6841 ttctttgata agctgcttca acatttgccc aagcttcttt cacttgttca tcaaggctaa
     6901 tcatcgtatt atacgagcaa ctagaaagca ttggcattac cattaataca attacaaatt
     6961 ttaatattct tttcatcatt ttaatttttt atagtataaa tttaataaaa ttatttatta
     7021 tttgagttat ttagtttact atgtttctta ctatatccaa aatagataac aaatccaatt
     7081 aataaccata caattaatcg catccaattt tcaacaccta agccaaaaat cattgcacca
     7141 caaaataaaa ttcctaaaat tggcactact ggtacaaatg gagttttgaa ttgtctaggc
     7201 atatctggtt caacttttct caatacaatt acagcaacac ataccaatat aaacgcaaac
     7261 aatgttccga tactagtcat gtgtccaaca acattacctg gtacaaatcc agcaaataaa
     7321 gctactaata ctaaaactgc taaagttcct ttgtatggtg ttttatattt aggatgtatt
     7381 tcagaaaatg ctttgggcaa taatccatca cggctcatag aataaaaaac tctactttga
     7441 cctaatagaa gtactaaaat tactgaagaa aagcccgcta atattgcaat tgtaatagat
     7501 ttgcttaacc aagtataggc aggcattgca gattgaatag ctgccgaaac agatgcctct
     7561 ccacctttat taggatctct aaaaaactca acactttcta atcctgttaa tacataagag
     7621 aataaaatat acaaaatagt acaaataatt aacgttccta taattccaac tggcattgcc
     7681 tttttgggat ttttagtttc ttgagcagcg gttgtgatgg catcaaaacc aatgtatgca
     7741 aaaaatactg ttccagccgc acctaaaatg cccattatcc caccaaaatt ataagtaatg
     7801 ccatcgtggt cagtatattt tgtaggttca ggaatataag gcgtatgatt cgctggattg
     7861 atataactcc aacctaaagt aataataagt aaaacaatag ccacttttac aataactata
     7921 atattattaa caaaagcaga ttcttgaatt cctttaatta gtaaaaagct tactgccgta
     7981 actattccaa gtgctggtaa attgacaatg ccttgcacac catcaacaga aacctgaaat
     8041 ggtgaatgac accattgata aggaattggg gcgacgccta atatttgtgt caaaaaatca
     8101 ttaaggtatt cactccaagc tattccaacg gtggctgctc caactgcata ttctaatacc
     8161 aaatcccaac ctattatcca tgctagaaac tctcccatcg tagcataagt ataggtatag
     8221 gcacttcctg aaattggaat agaagaagaa agttcagcat aacaaagtcc agccaaggca
     8281 caacctattc cagcgacaac aaaacctaag gttacagatg gtccagcatt ttgtgcagca
     8341 gctgcagccg ttctaacaaa aatcccagct ccaataacaa caccaattcc taaagatata
     8401 agtgctaacg aacttaatgt ccgtttgagt gaatcttctc cactattttc tgattctttt
     8461 aaaagttgtt gaacagattt tttagcaaat aattgcgaca tatttgattt ttaacaagca
     8521 atatacaaac actttcttgt catatcaaaa tatattggat agaaaattat tcttattcaa
     8581 aaaaaattac tcactaaaag gcaaattatc tttcagtttt ttgtgtaatt tttgattgta
     8641 ttcttctaat aaaaagttga aataattttt tgttgctttt gaaaggcaac taatatatac
     8701 atttagaata tattgaattt catcttgaat aatattaatt aattcaacag catctaaata
     8761 attggtgcag ttttctttaa ttctttcata atgcatatca acagccaaat ccaaaacagc
     8821 taaggttttc aatcctttta agtatagttt ttgatattcc ttttcggcat caaaatcaaa
     8881 attttgtgta ttcggaaatc tagccatcac ttcgtctgaa agtgtgaaca ttaaacctct
     8941 ttccattttt tgttctctat catacaaatg ctccaaaaat ctatcgggat taacaaatac
     9001 atcttgccaa tctatatgat cttgccaaat gcctaatctc tttgcattat ttcctaaatc
     9061 tataatagaa aattcagatt tattgggcaa tcgacggctt ccacgaccaa ccatttgatg
     9121 atataaagtt aaggagcgtg ttgctctatt caagataatt gtctcaaccg ttggctcatc
     9181 aaatccagtt gtcaatatac ccacacttgt aacaattgca tctggcgttt ctttcaacca
     9241 ttttaataca tctttacgct cttgtttatt gcttgtacta tccaaatggc ggattggaat
     9301 attatgaatt ttgaagaagt tttcaaccga ttttgaggta gcaatcgagc tattaaaaat
     9361 aagtgtttta gttccttttg ctttttcttt atacgcactc aacaatattt gatgcatatc
     9421 aaagcccata taaattctat ccaaagaatt tacagtgtaa tcaccatcaa taccgacttt
     9481 taagccaccc aattttacat caaaagtaaa tgttttggca ttactcaaaa atccattatg
     9541 aattaaatct ttgatagaat ctccaacaat taattttctg taattgcttc ttaatggata
     9601 atgaatatta gaacttaaag gcgttgcggt aacacctaaa ataactgcgt gttcaaaata
     9661 tttaaaaatt ttacgaaatg aattataatg tgcttcatcg acaataacta aatcaatacc
     9721 ttttaagaat tcatcatctt ctttcagtct attatttaat gtttcgacca ttgctaagaa
     9781 acaattataa tctttttgat cataaatatc ttctgtttcg ctagtaatca atttacattt
     9841 aaccgcagct tcttctaaag ctactgaagt ttgttttagt aattcaattc tatgagtaag
     9901 aattaaaatt ttcttttctt gatgttcaat atatcttcta gcaatttccg aaaagatgat
     9961 agttttacca ccaccagtcg gtaattgaaa tacaatatta tctctgcttt cattctctac
    10021 caatgcctca taaatgtttt ttatataggc ttcttgatag ggataaagct gcttagttct
    10081 cacaaatacg tcttcgattt caacagattt cattagctta ctttgctctt tcattaaaaa
    10141 aaatgcgagt cgcaagataa gcatttaatc ctgaatcata tcattaaaag atattatatt
    10201 attgtatcct ttagagtgaa aatatttttt aacataatcc atatctaaat catttgaaac
    10261 taatacaaaa tcgccacccc aagctcctaa agattttacc acaccattaa aatcactaaa
    10321 atatttatct tgaacttttt ctaaggataa aaaacgacta atgatatttt cgtgttgaat
    10381 taaacattta tcaaattcaa atttatccgt agtgttgcta ataatttttg ttaactcatt
    10441 tagttgctga ataattgctt gtctatcctc aatttttgtc tgtttataat attgaattgc
    10501 agttcttgaa ttttgttttt gattgaggta aacaaaatat aattgatttt taaaaggagg
    10561 attaaagttg cacagctcta cttttatttc attacattca tttttggaaa atagaatagg
    10621 tttataggct gttgcacatg caatatcata tccagaacct ccaaatgttt gttctaataa
    10681 ttggtatgga tttatatcaa accattgggc taccaaactg attaatgttg aacttgttcc
    10741 caagccccaa tgacgattaa aattcaaata cgttgaagat tttacaccat gaacgctatt
    10801 attttttttt aatattgatg cggttttcaa aattttacga agcatttcag acacttcaat
    10861 atctgaataa tctattaaat taaggtgttt gtcaaaatta caataaaacc aaatttctcc
    10921 attttcatca taacttttcc attctaaaat ttcagattga atagtttgta ggactaattt
    10981 ctgatgatat ttagtgggta atgctaaacc tttagcacca tctaaaataa agtattcagc
    11041 tgttaatagc aatttcccat tggcagtata tgaagcatca accaacatca aaaattattg
    11101 tcttaaaaat tatcaattaa gcaaaagctt catcaacaat tgccttctgt tcttgagcat
    11161 gtattttaga aaatccagtt gcagtagaag cactttgggc tctgctaatt aatttcaaat
    11221 cttttccatt tggataatta gacaagacaa atgtccaagc tcccatattt tttggttctt
    11281 cttgcaccca aacaaattcg gcatttttat atttatctaa aatagcttga tattgttttt
    11341 ttgctaaagg ataaatttgt tctacacgaa caatcgcaat atcttttcta tccgttgatt
    11401 cttttctttc taacaaatca taatatactt tacctgaaca caataaaaca cgcttaactt
    11461 ttg
//
LOCUS       DAOQCW010000079        11446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_2124_length_11446_cov_6.087086, whole genome shotgun sequence.
ACCESSION   DAOQCW010000079 DAOQCW010000000
VERSION     DAOQCW010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11446
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2124_length_11446_cov_6.087086"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..1642
                     /gene="atpA"
                     /locus_tag="PLS16_07985"
     CDS             65..1642
                     /gene="atpA"
                     /locus_tag="PLS16_07985"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNJ61058.1"
                     /translation="MVEVRPDEISAILRKQLGNVTTEAELEEVGTVLQVGDGIARIYG
                     LTEVQAGELVEFENGIKAIALNLEEDNVGVVLMGPSDAIKEGDKVKRTKQIASINVGE
                     GMLGRVINTLGEPIDGKGPISGQTYRMPIERKAPGVIFRQPVTEPLQTGIKAIDAMIP
                     VGRGQRELIIGDRQTGKTAIAIDTIINQKEFYERGEPVFCIYVACGQKASTVANIANT
                     LEANGAMPYTVIVAANASDPAPLQFYAPFAGAAIGEFFRDSGRPALIVYDDLSKQAVA
                     YREVSLLLKRPPGREAYPGDVFYLHSRLLERAAKIIAKDEIAARMNDLPESLKGIVKG
                     GGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLESNLFNAGVRPAINVGISVSRVG
                     GNAQIKSMKKVAGTLKLDQAQYRELEAFAKFGSDLDAATKSVLEKGKRNVEILKQPQY
                     SPLTVEKQVAIIYLGSKGLLRNVAVNKVREFEDTFLSTIEQKHPDVLANLKKGKLEDA
                     DLAKLEAVAKDLEGSFA"
     gene            1707..2594
                     /gene="atpG"
                     /locus_tag="PLS16_07990"
     CDS             1707..2594
                     /gene="atpG"
                     /locus_tag="PLS16_07990"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNJ61059.1"
                     /translation="MGANLKEVRGRIASVKSTIQITKAMKMVSAAKLKRATDRIVQIR
                     PYADQLNNVLQNLVSNLQGEVSISYGKQREGNKVLIVVITSDKGLCGGFNANSIKAAK
                     NLLADKYATLNEAKKVDVLTIGKRAYEAFKDGKNYVNRDFTELFHNLNFEDTSKAAEY
                     IMYAFDQGVYDKVEVVYGKFKNAGTQITTVEQFLPISTTTEASNSKLNADYIFEPSQV
                     EILEGLVPKILKTQFFRYLLDSNASEHGARMTAMDKATENANELLKTLNIMYNRARQA
                     AITTELSEIVSGAAALSNE"
     gene            complement(2703..3884)
                     /locus_tag="PLS16_07995"
     CDS             complement(2703..3884)
                     /locus_tag="PLS16_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61060.1"
                     /translation="MKNNFLKNIIAKKRLLLILIVFIINLYLMIMFYAYGATNYISTI
                     AFYFFIILLIYNIIKNKKIAFNIIASLIILFIAETYLRVSRKGNLNYTEANSSSIFAN
                     YNSQIYGENKKYFLGFRVNQPNSTVDYSSIDFSYKHQYNELGLRERPLVDFKNTKNIL
                     LLGDSFTEGAGAPDDSTLCKSLAFHLGQNIHCINAGVRGSDPVYEYQLLDTLFQITNP
                     KMVICNISYSDIPDIMNRGGDERFTNGFRKQFWWEYLYGISFLFRKVMLDVFEYRPFQ
                     IQFEDLTYSVDVIMRKMMAFEQYCNTKGIPFLCMITPNMNELIDRESFNFQQSFIELY
                     KHTGIRFINTQIGLTTYLNNNKMSPDNIYHLTDFHLNSKGYWLQGAIVADFIKGGMKM
                     E"
     gene            complement(3874..5817)
                     /locus_tag="PLS16_08000"
     CDS             complement(3874..5817)
                     /locus_tag="PLS16_08000"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002937.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HNJ61061.1"
                     /translation="MSVLWSPSEQFINNSNLYKFQKYIENKYQLSFHNYQEIWEWSVQ
                     NLEAFWEAVALFFDLKFHQTYSSVLKNTDKMYETKWFDGATISYTEHVFRNKNNENPA
                     IIYKQENQASIAVSWQELEQKVSAVQQFLKSKNIQKGDRIVGYLPNNVEAIIAFFACN
                     SLGAIWSCCSPDFGTESIVDRFQQIEPKLFIAASTYNYNGKSFNKIATIEEIASKIPS
                     LENVILIGEQHNNFINWASILEQDSKDLEFTPVEFQHPIWILYSSGTTGKPKGIVHST
                     GGNLIEHYKALALHQDCKKGERYLWYSTTGWMMWNYALSSLLVGCTLCIFDGAPNYPD
                     FYSIWEFIDTEKINHFGIGAAFYLSCKKEHLAVNKKYNLTHLRSLGSTGSPLPADGFE
                     YIYQHIKKDVWLISLSGGTDICSAFVGGSPYLSVYAGEIQCRMLGASVEAWNESGKAV
                     EQELGELMLTKPMPSMPVYFWNDANNEKYKSAYFEDNDNIWRHGDWIKITKHQGVIIY
                     GRSDATLNRDGVRIGTAEVYNAVEILEEIKDSLVICIERENGSFYMPLFVVLQDGIVL
                     NDELKTKIKNSLRKQYSPRHVPDEIIEVNEIPYTISGKKMEMPIKKIMMGMPIEKSIS
                     LDAMKNPNSIDEYLALQTKINEK"
     gene            complement(5891..6607)
                     /locus_tag="PLS16_08005"
     CDS             complement(5891..6607)
                     /locus_tag="PLS16_08005"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HNJ61062.1"
                     /translation="MLPIIYLIVAIIEISGEFLERFPIIYVAKPLLMPILMIWVYTTT
                     KNKKDILFILLGLFFSWLGDLFLMIRHEFEPENAKMFFIFGLAAFLIAHLNYIFCFLK
                     DVKGKYKIGFIESKPSLILPFIAFIVGLLYFLGDSIQAMKVPVYLYCIIITLMSIMAL
                     NRKGIVSTTSFNLIFIGSLLFIFSDMCIAISVFKQPFYLSRVIIMSTYILAQYLIAKG
                     YIIHINTAKNLPTNLKTELK"
     gene            complement(6617..7651)
                     /locus_tag="PLS16_08010"
     CDS             complement(6617..7651)
                     /locus_tag="PLS16_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HNJ61063.1"
                     /translation="MLNFQPNDIGIKNGHIFSLPHEYKDAELIILPVPWEVTVSYNTG
                     AAQGPEAILDASSQLDLFDPFLKDAHKYGVYLLQISEYWQQKNKQLRPKAAEYIRLLE
                     NGVQISDNKTMQNSLEEINTVCDELRAWVFEETDKIIRDGKMVALLGGDHSTPLGLIQ
                     ALAQHHNDFGILHIDAHADLRDAYEGFTYSHASIMYNAIQIEQVKKLVQVGIRDISPE
                     EFAITQDNSKLKTFFDWDLNDKKIEGIAWKNIVDEIIAELPENVYISFDIDGLQQMYC
                     PNTGTPVAGGLSFDEAIYLVRKVVDSGKKIIGFDLNEVAPNPNDKEWNANVGARVLFK
                     LCNQAIKSNL"
     gene            complement(7681..8403)
                     /locus_tag="PLS16_08015"
     CDS             complement(7681..8403)
                     /locus_tag="PLS16_08015"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HNJ61064.1"
                     /translation="MQNTAWLSRTELLIGEEKLQKLIQAHVLVVGLGGVGSYCAEFIA
                     RAGVGKMTIVDGDIVDDTNRNRQLPASISTIGKSKADWMAERIKDINPEIELTVIKTF
                     LSPEKAQEIITADKYNYVVDAIDSISPKLNLLIAAYRNKVKIVSSGGAGGKLDVTRIK
                     VADLSKTHDCPLVKYVRKRLKKEKIYKGFKVVFSPELQSEKRILMTDGSNFKRSSYGT
                     ISFMPAAFGGACASVVIQHIIK"
     gene            complement(8433..10196)
                     /gene="rho"
                     /locus_tag="PLS16_08020"
     CDS             complement(8433..10196)
                     /gene="rho"
                     /locus_tag="PLS16_08020"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNJ61065.1"
                     /translation="MHNAEQLNVLSVTDLKAIAESLLIPKTKISKLKKGELVELILKQ
                     ANNNTSEGLNNTTTLTPVDETQKEHTHRAIEQISIESESTQQNLEDLNDTKALNPEMN
                     QENAVANNDKKIRNRINKVVHKVATNSTPTAEIKESTTQTVQNKETIVEEKTEEKILE
                     NKPFEHKPNHNKHPKYPNHQNGNNNRNNNSESKNNEVVEELSPIIEEEKKEIFNIDLD
                     GVIPGEGVLEIIPEGYGFLRSSDYNYLSSPDDVYVSPGQIKLIGLKTGDTIVGTIRPP
                     KEGEKYFALLKVDSINGKTPQEIRDRIPFDYLTPLFPNEKLNLFQDERHLTTRVVDLF
                     TPIGKGQRGMIVAQPKVGKTMLLKDVANAIAKNHPEVYLIVLLIDERPEEVTDMQRSV
                     NAEVVASTFDEPADRHVKVSNIVLQKAKRLVECGHDVVILLDSITRLARAYNTVAPSS
                     GKVLSGGVEANALHKPKQFFGAARNIENGGSLTIVATALIETGSKMDEVIFEEFKGTG
                     NMELQLDRKLANKRIFPAIDVTSSSTRRDDLLLDKDTLSKMWILRNHLADMTSEEAMK
                     FLLNQMKGTRSNEEFLLMMNR"
     gene            10473..11417
                     /locus_tag="PLS16_08025"
     CDS             10473..11417
                     /locus_tag="PLS16_08025"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNJ61066.1"
                     /translation="MLFDSIWKDLKWHLKFGVPLSKIIYINVFIFLLLSFLNVIDFFS
                     KLHTSTWLLEQLSFFPSRQFFFQPWGILTYQFTHIGFMHILFNMLWLYWFGMIVQDFI
                     GRNKIVPLYLFGGFFGAVFFALITNLIQLADPNFNLNGSMHGASAGVMAIVWAAATLS
                     PNYEIRLLLLGNIKLKWIALAILVLDIIMLNDGNFGGHIAHIGGAFFGWLYITLLRNG
                     NDLTHPFYDVIDFFKSIFKKKEKMKVVYRKPQQQQKVYAGQEKKQQTNKKESEFSELN
                     KREKQEKLDNILDKINSSGYDSLTSAEKEFLFKISKED"
BASE COUNT         3836 a         1809 c         1750 g         4051 t
ORIGIN      
        1 gcaaatattg aattaatata atgcttgtca aacttattaa atttataaat taaaatataa
       61 taaaatggta gaagtaagac cagacgaaat ctcggctata ttacgcaaac aattaggtaa
      121 tgtaactaca gaagctgaat tagaagaagt aggtacagta ttacaagtgg gtgatggtat
      181 tgcacgtatt tatggattaa ctgaagttca agcaggtgaa ttggttgaat ttgagaatgg
      241 tattaaagct attgcattaa acttagaaga ggataatgta ggtgtggtat taatgggtcc
      301 atcagatgca attaaagaag gagataaagt aaaacgtacc aaacaaatcg catctatcaa
      361 tgtaggtgaa ggtatgttgg gtagagttat caatacttta ggagaaccta ttgatggtaa
      421 aggtccgatt agtggtcaaa cttatagaat gccaattgaa agaaaagccc ctggtgttat
      481 tttccgtcag ccagttacag aaccattaca aacaggaatt aaagctattg atgctatgat
      541 tccagtaggt agaggacagc gtgagttgat tattggagat agacaaacag gtaaaactgc
      601 aattgcaatt gatactatca ttaatcaaaa agaattttac gaaagagggg aacctgtttt
      661 ctgtatatat gtagcttgtg gtcaaaaagc atcaactgtt gctaatattg caaatacatt
      721 agaagctaat ggagcaatgc cttatacagt gattgttgcg gcaaatgcat ctgacccagc
      781 accacttcaa ttctatgcac catttgctgg tgctgcaatt ggagagtttt ttagagactc
      841 aggtcgtcct gctttaattg tttatgatga tttatctaaa caagcagtgg catatcgtga
      901 agtttcatta ttattaaaaa gacctcctgg acgtgaggct tatcctggag acgtattcta
      961 cttacactct cgtttattag aaagagcggc aaaaattatt gctaaagacg aaattgctgc
     1021 tagaatgaat gatttgcctg agtctttaaa aggtattgta aaaggtggag gctctttaac
     1081 agcattgcca atcattgaaa ctcaagcagg tgacgtttct gcttatattc caaccaacgt
     1141 aatttcgatt actgatggtc agatattctt agagtctaac ttgtttaacg ctggtgttcg
     1201 tcctgcaatt aacgtgggta tttctgtatc tcgtgtgggt ggtaatgcac agattaaatc
     1261 aatgaaaaaa gtggctggaa ctttaaaatt agaccaagca caatatagag aattagaagc
     1321 atttgctaaa ttcggttctg acttagatgc tgcaacaaaa tcagtattgg agaaaggtaa
     1381 acgtaacgtg gaaatcttaa aacaaccaca atattctcca ttaacagtag aaaaacaagt
     1441 tgcaattatc tacttaggct ctaaaggttt attgagaaat gtagctgtta ataaagtaag
     1501 agaatttgaa gatacgttct tatcaacaat agaacaaaaa caccctgatg ttttagcaaa
     1561 cttgaaaaaa ggaaaattag aagatgcaga tttagctaaa ttagaagcag tagctaaaga
     1621 tttagaagga agttttgcat aattaaatta tagttggagg atatagccat atattctcca
     1681 acaaaattga aatgtaatta ttaaaaatgg gagcaaattt aaaagaagta agaggaagaa
     1741 tagcatctgt aaaatcaact atacagatta caaaagctat gaaaatggtt tctgctgcta
     1801 aattaaaaag agcaacagat agaattgtcc aaatacgtcc ttatgctgac caacttaata
     1861 atgttttaca aaatcttgtt agtaacctac aaggagaagt ttcaatttct tatggtaaac
     1921 aaagagaagg aaacaaagtt ttaattgttg tgattacttc tgataaaggt ttgtgtggtg
     1981 gttttaatgc caattctatt aaagctgcta aaaatttatt agcggataaa tatgctactt
     2041 taaacgaagc aaaaaaagta gatgttttaa cgataggcaa aagagcttat gaagcattta
     2101 aagacggtaa aaattatgta aaccgtgatt ttacagaatt atttcataat ctaaactttg
     2161 aagatacttc taaagctgct gaatatatca tgtatgcttt tgatcaaggc gtttatgata
     2221 aagtggaagt tgtttatggt aaatttaaaa atgctggtac tcaaattaca acggtagaac
     2281 agtttttgcc aattagtaca actacggaag catctaattc aaagttaaac gctgattata
     2341 tttttgagcc ttcacaagtt gaaattttag aaggattagt acctaaaatt ttaaaaactc
     2401 aattctttag atatttgtta gattctaatg cttctgaaca tggagcgaga atgacagcga
     2461 tggataaagc cactgaaaat gcaaatgaat tgcttaaaac attgaacatt atgtacaata
     2521 gagcaagaca agcagctatt acaacagagc tttctgaaat cgtttctggt gcagcagcac
     2581 taagcaatga ataattacta atgctttagt atttcaagat ttctaattct atttcttttt
     2641 ctattattaa gtaatatgtt ttggatactt ttgtatcaat ctaaaatttt gaaacctaaa
     2701 ttttattcca ttttcatacc acctttgatg aaatctgcaa caatagctcc ttgtagccaa
     2761 tatcctttgc tgtttaaatg aaagtctgtt aggtgataaa tattatctgg cgacattttg
     2821 ttattgttta aataagttgt taatccgatt tgcgtattaa taaatcgaat tccagtatgt
     2881 ttatataact ctataaaact ttgctgaaaa ttaaaacttt ctctatctat taattcattc
     2941 atattgggtg taatcataca taaaaaggga atcccttttg tattgcaata ttgctcaaat
     3001 gccatcattt tccgcataat aacatcaaca ctatacgtta aatcttcaaa ctgtatttga
     3061 aatggtcgat attcaaatac atctaacatt acttttctaa agagaaaaga aataccatat
     3121 aaatattccc accaaaattg ttttctaaat ccattagtaa aacgttcatc accaccacga
     3181 ttcataatat caggaatgtc agaataagat atattgcaaa tgaccatttt tggattagtg
     3241 atttgaaata aagtatctaa taattgatat tcatagactg gatcgctccc tcgcacgccc
     3301 gcattgatgc agtgtatatt ttgacctaaa tgaaatgcta aagatttaca taaggttgag
     3361 tcatcaggag caccagcacc ttctgtaaag gaatcaccta atagtaaaat attttttgtg
     3421 tttttaaaat ctactaatgg tctttctctc aaacccaatt cattatattg atgtttatat
     3481 gaaaaatcta tagagctata atcaactgta ctatttggtt gattgactct aaatcctaga
     3541 aagtattttt tgttctctcc ataaatttgt gagttgtagt ttgcaaaaat agagcttgaa
     3601 ttagcttcag tataattaag attgcctttt ctagacactc gcagataggt ttctgctatg
     3661 aataaaataa ttaaacttgc tatgatatta aatgctattt ttttattttt tataatatta
     3721 tagatgagta atattataaa aaaataaaat gcaattgtag agatataatt agttgcacca
     3781 taagcataaa acattatcat taagtataaa ttaatgatga atactattaa aatcaataat
     3841 aatcgttttt tggcgattat attttttaaa aaattatttt tcattgattt ttgtttgtaa
     3901 tgcaagatat tcatcgatgc tatttgggtt tttcattgca tctagtgaaa tacttttttc
     3961 aattggcata cccatcataa ttttctttat aggcatttcc atttttttac cacttatagt
     4021 ataaggaatc tcattaactt ctataatttc atcaggtaca tgtcgaggag aatattgttt
     4081 tcgtaaacta tttttaattt ttgtctttaa ctcatcgttt aaaacaatgc catcttgcaa
     4141 aacaacgaat agcggcatat aaaaactacc attttcacgt tctatacaaa ttactaaact
     4201 atcttttatt tcttctaata tttctacggc attatatacc tcagcagtgc caattcttac
     4261 gccatcgcga tttaatgttg catcacttct gccatatatt ataacaccct gatgttttgt
     4321 gatttttatc caatcgccgt gtcgccaaat attatcgtta tcttcaaaat aagcactttt
     4381 gtatttttca ttattagcat cattccaaaa ataaactggc atactcggca ttggtttagt
     4441 gagcatcaat tcgcccaatt cttgttcaac agccttacct gattcattcc aagcttcgac
     4501 actcgcccct aacattcgac actgaatttc accagcatat acggataaat aaggacttcc
     4561 accaacaaaa gcactacaaa tatcagtgcc gccacttaga gaaattaacc aaacatcttt
     4621 tttgatatgc tggtatatat attcaaatcc atctgcaggt aacggagagc cagtagaacc
     4681 taaagaacgt aaatgtgtta agttatattt cttattgacg gccaaatgct cttttttaca
     4741 agacagataa aaggcagctc caattccaaa atggttaatt ttttcggtat ctataaattc
     4801 ccaaattgaa taaaaatctg gatagtttgg tgctccatca aaaatacata aagtgcaacc
     4861 taccaaaagc gaagaaagtg cataattcca catcatccaa ccagtagttg aataccacaa
     4921 atacctttct ccttttttac aatcttgatg taatgccaat gctttgtaat gttctattaa
     4981 atttccacca gtactgtgta ctattccttt tggttttcca gttgttccag aagaatataa
     5041 aatccaaatt ggatgttgaa attcaactgg cgtaaattct aaatcctttg aatcttgttc
     5101 taaaatacta gcccaattaa taaaattatt gtgttgctct ccaatcaaaa ttacattttc
     5161 taatgacgga attttagagg caatctcttc aatagttgct attttattaa atgatttacc
     5221 attgtaatta taagtagaag cagcgataaa taacttcggt tctatttgtt gaaatctatc
     5281 gacaatgctt tctgttccaa aatcaggaga acaacaagac caaattgcac ccaaactatt
     5341 gcatgcaaaa aaggctataa ttgcttcaac attatttggt aagtaaccga cgattctatc
     5401 gcctttttgt atattttttg attttaaaaa ttgttgaact gcactaactt tttgttctaa
     5461 ttcttgccat gaaacagcta tgcttgcttg attttcttgc ttgtaaataa tagcagggtt
     5521 ttcattgttt ttatttctga aaacatgttc tgtatagctg atggtcgctc catcaaacca
     5581 ttttgtttca tacattttat ctgtattttt taaaacagat gaataagttt gatgaaactt
     5641 taaatcaaaa aaaagagcaa ctgcttccca aaaagcttct aagttttgta cactccattc
     5701 ccaaatttct tggtagttat gaaatgatag ttggtatttg ttttcaatat atttttgaaa
     5761 cttataaagg tttgaattat taataaactg ttcagaaggc gaccaaagta cagacatctt
     5821 ttttttcttt aaaaataact atctttttta aataaagcta tatttatcaa cctaataaag
     5881 attaatcaca ttatttcaat tctgttttta gatttgtcgg cagatttttc gctgtgttaa
     5941 tatgtatgat atagcctttt gcaataagat attgagctaa aatataggta ctcattatta
     6001 ttactctact taaataaaaa ggttgtttga aaactgaaat agcaatacac atatcagaaa
     6061 atataaataa caatgaacca ataaagatta gattgaatga agtagtagaa actatacctt
     6121 ttctatttaa agccataatg gacatcagtg taatgatgat acaatataaa tatactggta
     6181 ctttcattgc ttggatgcta tctcccaaaa agtataaaag cccaacaata aatgcaataa
     6241 aaggcaatat taatgacggt tttgactcaa taaatccaat tttatattta ccttttacat
     6301 cttttagaaa acagaaaata tagtttaaat gagcaattag aaaggctgct aatccaaata
     6361 taaaaaacat tttagcattt tctggttcaa attcatgtct tatcatcaaa aataaatcac
     6421 ctaaccatga gaagaataaa cctaataata taaacaaaat atctttttta ttcttagtgg
     6481 tcgtataaac ccatatcatt aaaatcggca ttaataaggg ctttgcaaca taaataatgg
     6541 gaaaacgctc taaaaactct ccagaaattt caattatggc aactattaaa tagatgatgg
     6601 gtaacatagc ttatttttaa agattacttt ttattgcttg attacatagc ttgaataata
     6661 ctctcgctcc aacattggca ttccactctt tgtcatttgg atttggtgca acttcattca
     6721 aatcgaatcc gataattttt ttaccactat ccacaacttt tcttaccaaa taaattgcct
     6781 catcaaaaga taagccacca gcaactggtg ttccagtatt tggacaatac atttgttgta
     6841 agccatcaat atcgaagcta atataaacgt tttcaggcaa ttctgctata atctcatcaa
     6901 caatattttt ccaagcaatt ccttctattt ttttgtcatt caaatcccaa tcaaaaaatg
     6961 tctttaattt agaattatct tgtgtgatag caaattcttc gggagaaata tctctaatac
     7021 ccacttgtac taattttttc acttgctcaa tctgaatagc attgtacata atcgaagcat
     7081 gcgaataagt aaatccttca taagcatctc tcaaatcagc atgtgcatca atatgtaaaa
     7141 ttccaaaatc attgtgatgc tgtgccaaag cttggattaa accgagcggt gtactatgat
     7201 ccccacctag tagagccacc atttttccat ctctgataat cttgtctgtt tcttcaaaaa
     7261 cccaagctct caattcatcg caaacggtat ttatttcctc caaactattt tgcattgttt
     7321 tattatcaga aatttgtacg ccattttcta ataaacgaat atactcagcg gcttttggtc
     7381 gaagttgttt gtttttctgt tgccagtatt cagatatttg caaaagataa actccatatt
     7441 tatgagcatc tttcaaaaaa ggatcaaata agtctaattg agaactggca tctaaaatag
     7501 cttcaggacc ttgtgccgct ccagtattat aagaaaccgt aacttcccaa ggaacaggca
     7561 gtataatcaa ttcagcatct ttatattcat gtggcaatga aaatatatgt ccatttttta
     7621 tacctatatc attaggttga aaatttaaca tgattaattt ttttataaaa ataggaaaaa
     7681 ctatttaata atgtgttgaa taacaacact tgcacaagca ccaccaaaag cggcaggcat
     7741 aaaagaaata gtaccataag aagagcgttt gaaattgcta ccatcggtca ttaaaattct
     7801 tttttcactt tgtaattcag gcgaaaaaac aaccttaaat cctttgtaga ttttttcttt
     7861 tttcaatctt ttacgtacat attttactaa aggacaatcg tgtgttttgg ataaatcggc
     7921 tactttaatt ctcgtaacat ctaattttcc accagcacca ccactggaaa caatcttaac
     7981 tttattgcgg taagctgcta ttaatagatt taattttggt gaaatactat ctatggcatc
     8041 gacgacataa ttatatttat cagcagtaat aatttcttgt gccttttctg gagataaaaa
     8101 agttttaatt acggtcaatt caatttcagg attaatgtct ttaattcgtt cagccatcca
     8161 atctgctttg gatttcccaa tcgtagaaat agacgctggc aattgcctat ttctgttcgt
     8221 atcgtccaca atatctccat caacaatggt catttttcca actccggctc gtgcaataaa
     8281 ttcggcacaa taagagccaa ctccgcccaa accaaccact aaaacgtgtg cttgaattaa
     8341 tttctgtaac ttttcttctc caatcaaaag ttctgttctg gaaagccaag ctgtattttg
     8401 cattgcttaa aaagaataat aattacaaaa atttatctat tcatcatcaa taaaaattct
     8461 tcattgctac gagtcccttt catttgattc aataagaact tcatagcttc ttcagaagtc
     8521 atgtctgcca agtgatttct tagaatccac attttggata aagtatcttt atctaataat
     8581 aaatcatctc ttctggttga tgaagaggta acatcaatcg ctgggaaaat tcttttattg
     8641 gctagttttc tatctaattg caattccata tttccagtac ctttaaattc ttcaaagata
     8701 acttcatcca ttttggaacc tgtttcaatt agtgctgttg ctacaatagt taatgaacca
     8761 ccgttttcaa tatttcttgc tgcaccaaaa aactgttttg gcttgtgtaa tgcatttgct
     8821 tcgacaccac ctgacaatac ttttccagat gatggagcaa cggtattata agctcttgct
     8881 aatcttgtaa ttgaatccaa aagaattaca acatcgtgtc cacattccac taatcttttt
     8941 gctttttgta atacgatatt agatacttta acgtgtctat ctgctggttc atcaaatgta
     9001 gaagcaacaa cttctgcatt aacactgcgt tgcatatctg taacctcttc aggtctttcg
     9061 tcaattaaaa gaacaattaa atatacttca ggatggtttt ttgcaattgc attggcaaca
     9121 tcttttaata gcatcgtttt tccaactttt ggttgggcaa caatcattcc tctttgtcct
     9181 ttaccaattg gtgtaaataa atcaacaact cttgtggtta gatgtctttc atcttggaat
     9241 aagtttaatt tttcgttcgg aaacaaaggc gttaaataat cgaaaggaat tctatctcta
     9301 atttcttgtg gtgttttgcc attgatgcta tccactttta ataaggcaaa atatttttca
     9361 ccttcttttg gaggtcgaat tgttccaaca atagtatcgc ctgtttttaa gccgattaac
     9421 tttatttgtc ctggagaaac atatacatca tctggagaag aaagatagtt gtaatctgat
     9481 gagcgtaaga aaccatagcc ttcagggata atttctaaaa ctccttcgcc aggaattaca
     9541 ccatctaaat caatattaaa tatttctttt ttttcttctt caataatagg cgaaagctcc
     9601 tcaactactt cattgttttt tgattcagag ttattgtttc tattattatt tccattttga
     9661 tgattaggat attttggatg tttgttgtga ttaggtttgt gttcaaaagg cttattttca
     9721 agtattttct cttcagtttt ttcttcaaca atagtttctt tattttgtac tgtttgagtg
     9781 gtactttctt ttatttcagc cgtgggtgta gaatttgtgg caactttatg aacaactttg
     9841 ttgattctat ttcttatttt tttatcatta tttgctacag cattctcttg attcatttca
     9901 ggatttaacg cctttgtatc attcaaatct tctaaatttt gttgagtaga ttccgattca
     9961 atactaattt gttcaatagc tcgatgagta tgttcttttt gagtttcatc aacaggagtt
    10021 agggtagttg tgttattaag accttcagaa gtattgttat ttgcctgttt taaaataagc
    10081 tctaccaatt cgcctttttt taatttgcta atctttgtct taggaataag cagtgattct
    10141 gcaattgcct ttaaatccgt taccgataaa acgtttagtt gttctgcatt atgcatataa
    10201 gaataaataa aatttgtgtt ttaaaaacat gtattgtcta aaataaaatg tcaataggaa
    10261 gtttgcaatg aataattgct cgacttgttt tgatgttgca aagtaaatac tttttttata
    10321 aaaaaactat ttttattata aaaatctctt gaatctacta aatctcttaa tttttaaaga
    10381 ttattaaaag gattcgttat agcactttta agatttttta ttagtataag tttgcctttt
    10441 tgtgtatttt gtaattcgaa atttaactat caatgttgtt tgattcaata tggaaagatt
    10501 taaagtggca cttaaagttt ggtgttcctt tgtctaaaat tatatatata aatgtattta
    10561 tttttctatt gttgagcttt ttgaatgtga tagatttctt ttcaaaactt catacaagta
    10621 cttggttgtt agagcaactt tcattttttc cttccagaca atttttcttc caaccatggg
    10681 gaattttgac ttatcaattt acacacattg ggtttatgca tattttattt aatatgttgt
    10741 ggttgtattg gtttggaatg atagttcaag attttattgg cagaaataag attgtaccat
    10801 tatatctttt tggaggtttt tttggtgcgg tattttttgc tttaattacg aatttaattc
    10861 aacttgcaga tccaaacttt aatttgaatg gctctatgca tggtgcgtct gctggagtga
    10921 tggctattgt ttgggcggca gctacattat caccaaatta tgaaataaga ctattgctat
    10981 taggaaatat aaaattgaaa tggatagcct tagcaatttt ggtattagat attattatgc
    11041 taaatgatgg aaattttggt ggacatattg cccatattgg cggtgcattt tttggctggt
    11101 tatatattac attgcttaga aatggcaatg acttgacaca tccattttat gatgttatag
    11161 atttttttaa atcaattttt aagaaaaaag aaaaaatgaa agtggtttat agaaaacctc
    11221 aacaacaaca gaaggtttat gctggtcaag aaaaaaagca gcaaacgaat aaaaaagaaa
    11281 gtgaattttc tgagcttaat aagcgagaaa aacaagaaaa attagacaat atattagaca
    11341 aaataaattc atcgggatac gatagtctga caagtgctga aaaagaattt ttattcaaaa
    11401 taagtaaaga agattgaaat tcttacattt gattatatca tttttc
//
LOCUS       DAOQCW010000080        11437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_2129_length_11437_cov_5.391407, whole genome shotgun sequence.
ACCESSION   DAOQCW010000080 DAOQCW010000000
VERSION     DAOQCW010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11437
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2129_length_11437_cov_5.391407"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            343..4662
                     /locus_tag="PLS16_08030"
     CDS             343..4662
                     /locus_tag="PLS16_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNJ61067.1"
                     /translation="MLDRIILFSIKNKIIVGFFTLILIVWGIWSATKLPIDAIPDITN
                     NQVQIITVTPTLASQEVEQLITFPIEQSIANLPDIEETRSISRFGLSVVTVVFKDKVD
                     IYFARQLISEKLKEAQEDIPAGLGKPEMAPVSTGLGEVYQYIIHPKKGSENKYSSKDL
                     REMQDWIVARHLNGTPGVAEINSFGGELKQYEVAINPDRLIAMNVNISEVFSALEGNN
                     QNTGGAYIEKQPNVFFIRGIGTVNSLDDIGNIMIKNINGVPSFIKDVADVRFGHAVRY
                     GALTYNGEVDAVGGVVMMLKGENSNDVVKRVKEKMLVIQKSLPDDVVIEAYLDRTNLV
                     NRAIHTVEKNLLEGALIVIFILVVFLGNFRAGLIVASVIPLAMLFALSLMNLFGVSAN
                     LMSLGAIDFGLIVDGAVIVVEATMHHLGLRKSVHRLTQSEMDDEVFNAASKIRKSAAF
                     GEIIILIVYIPILSLVGIEGKMFRPMAEAVGFAILGALILSLTYVPMMSALFMPKSTN
                     PKASFSDKMMEKFQQIYQPLLEKAIQLKYIIITISIILFSISILLFGRMGGEFIPNLK
                     EGDFAFHCILPQGTSLNQSIETSMQASRIIKQFDEVKMVVGKTGTAEVPTDPMPFEIT
                     DLIIVLKPQSEWKNNKTYEDLADEMMEKLEDLPGVFFESSQPIQMRFNELMTGVRQDV
                     AVKIFGENMDTLSAYANRVARVIKNIDGATAPQVERVDGLPQINIEYDRLRLANYGLS
                     IQNINDIVSMAFAGKSSGIVYENERRFDLVVRLDSIHRDNIDNVRNLMIPISSGMQIP
                     LSQVADINYKLAPAQISREAGKRRIVVGFNVKDRDVQSVVEDIQKTLDEQVKLPTGYY
                     YTYGGTFENLQAASKRLMIAVPLALLLIFVLLYFTFHSVKLAVLIFTAIPMSAIGGVI
                     ALLIRDMPFSVSAGIGFIALFGVAVLNGIVLIGTFNQLRKEGETDILKIVVEGAKIRL
                     RPVLMTASVASLGFLPMALSSSAGAEVQRPLATVVIGGLITATLLTLFVLPLLYLIFN
                     SKESMRNKIQSKSIVMVLFILFFGFSGSNLYAQAERKSLDELLQLSSNNLQYQINKTQ
                     KTQSAAAIKGVQRINKTGVFIENEDIRPSDRIGIWKVGLSQDIPWPGVYRSQKKLAQE
                     QSKLFDVGEHLISAELKRDITASYYEMWYVQDKMKLYEKMDSIYSTLHLATKLKVKTG
                     DSPGLDSVFSKIKMNENFVLLKQLNQDIAKVQKQLMLELNTDQVYLPENKPLSKIELS
                     FDNTGLHPELQAQQQQIAIAEKTVNVVKQENLPEMSTRVFSQRLYGLKDPYTGFSVNI
                     AFPIFGNGAYKSRLKAAQTSVHLQEQELEYNKQQFDVQKVQLLLEVEKNTQLLDFYET
                     EGLKQAENIINSALLSYQTGDISFADLNEYLTQTIQIKQRYIDALNLYNQSVIQYNYY
                     NNK"
     gene            4670..5938
                     /locus_tag="PLS16_08035"
     CDS             4670..5938
                     /locus_tag="PLS16_08035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNJ61068.1"
                     /translation="MKIQFKILPFILALLMFNSCKNNHEDGHNHEAETSAEQPNTAEE
                     SHSVTALTDEQIKAAGISYGTIEMKSLTAVLQANGNIKVPNNNKAMITSLYGGVIKSI
                     HVESGKIIAKGQVIATIANPQFIQLQEEYLTINSKITFAEQELQRQTELSEGNAGAKK
                     NLQSITAELNGLKIRKASLFQQIQLMGINPSTINNSNLKTTINVLSPINGVVSTLFAK
                     IGSYVDVAAPIAEIIDNQAMHVDLNIFEKDISNIKLGQSIIFRTINNPDNQYEAVVHT
                     IGSSFENETKSIAVHCDVKGNRKGLIDGMSVVASISLHNILTASVPDAAIVNFEGKDF
                     IFIITDKIPEIEKHEDTDEHGHQHEPKKTENENIKTTNFERIEVLKGVSNMGFTAITP
                     VVTLPENVKIAVKGTFFINAKLVNTGEHEH"
     gene            5962..8001
                     /locus_tag="PLS16_08040"
     CDS             5962..8001
                     /locus_tag="PLS16_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNJ61069.1"
                     /translation="MEHQHIYDKDGNQLCCTMEKKIYLNAGAQKLLGNNSEQHEHDHH
                     HDHSGKLLFLLPLLSLVLLLTALYFDHILKPIWFKETFRIIWYVAAYLPVGLPVVKES
                     LESILKKDFFSEFTLMVIATIGAFAIGEFPEAVTVMLFYAIGEVFQTLAVKRAKLNIK
                     SLLDQRPDEVTILENNIAKVVKAELVEIDSVIQLKPGEKLGLDGVLISDSASFNTAAL
                     TGESKPDSKQKGESVLAGMINLNTVTQIRVTAKYADSKLSRILELVQNASAQKAPTEL
                     FIRKFARIYTPIVVNLALLIVVLPYLLHLTNVQFLPDSIQHYSFQNWLYRALVFLVIS
                     CPCALVISIPLGYFGGIGAASRHGILFKGSNFLDIIADIQHVVMDKTGTLTEGVFQVQ
                     EVVFNSLFDKDKILQFVNVIESHSTHPVATAILQYVGERSPDIKIDNIVEISGHGLKG
                     MVDDKELLVGNFKLMDKFNIQYNLDPNSIIYTLIAVAYDGQFVGYITIADSIKADASK
                     TIQALHTMNVKTTMLSGDKHTVVQYVAKALNIDNAFGDLLPEDKVEKVKQIKNSYKSV
                     AFVGDGVNDAPVVALSDVGIAMGGLGSDATIETADVVIQDDMPSKIPMAIKIGKATKK
                     IVWQNIILAFGVKLIVLILGAGGVATMWEAVFADVGVALLAILNAIRIQRMNFDK"
     gene            7991..8542
                     /locus_tag="PLS16_08045"
     CDS             7991..8542
                     /locus_tag="PLS16_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61070.1"
                     /translation="MINSREVNFCKIISTDIGIINEIYAVYTRSFPEDERRDEKQFYA
                     LFQNPKVQVFLVVFEHNAIAYLITWQLEEFVFIEHFEVFETYRNMKYGALILNQFSEK
                     FSKIILESEPEIMGELAIRRLGFYGRNGFSIVERNYEQPPYSKEKNALKLYLLSNFKI
                     DAIAPIVLEIYKVVYQKNFNYLY"
     gene            complement(8539..9672)
                     /locus_tag="PLS16_08050"
     CDS             complement(8539..9672)
                     /locus_tag="PLS16_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNJ61071.1"
                     /translation="MDLFKVYPLLDVELESGKGVFVFDKNGQKYLDFYGGHAVISVGH
                     AHPYYIKALNEQLEKLVFYSNSVHLEIQQKLAQKLGQISGYANYNLFLINSGAEANEN
                     ALKLASFYNKRKKVIAFSGAFHGRTAAAVAVTDNPKIKPAINPDDHVIFLPFNDNVAL
                     EKTFKEQGNEICAVIVEPIQGVNGIYEASASFLEKIQELCHQNDAIFIADEVQCGFAR
                     SGKFFAHQFANVQPDIITIAKGMGNGFPVGGVLINEKIQAVYGMLGTTFGGSPLACAA
                     TLAVLDIIENENLIENTQKMGQYLLENLKGIKGLKQIRGKGLILGLEFEEPIKDLRNN
                     LLFKHQIFTGNSNNPNTLRVLPSLAIQKEEIDLFLNALKKETN"
     gene            complement(9766..10821)
                     /locus_tag="PLS16_08055"
     CDS             complement(9766..10821)
                     /locus_tag="PLS16_08055"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF009897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HNJ61072.1"
                     /translation="MFPIKIEQAQHSKIDTIDFNDIKFGRTFSDHMLVAEYYDGAWQS
                     AVIKPYGNIELSPACNVFHYGQAIFEGMKAFYKADESGVVLFRPEMNWERMNRSAKRM
                     SMPEIPKDLFIEGTKKLIELDKAFVPNVPNSALYIRPVMFASEPFIGVKTSDRYLFII
                     MTGPVGAYYGEPISVYVHEKYIRAAKGGYGDAKAAGNYAGSLYPLEVARQNGYKDVLW
                     LDGEHKQYVQEVGTMNIFFVINNKLVTPMLDGSILAGVTRNSIITIAKELGYEVEEKL
                     IDIEDVIAAHQNGTLTEVFGTGTAVVVNAVDKIGYQGKDYKLDATQFKVAQQLKEKLV
                     NIQKGIDTDTHHWIVNC"
     gene            complement(10898..11035)
                     /locus_tag="PLS16_08060"
     CDS             complement(10898..11035)
                     /locus_tag="PLS16_08060"
                     /inference="COORDINATES: protein motif:HMM:NF040113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61073.1"
                     /translation="MNEILLSNHKLVAVFAVILIILIGMLFYLFSIDKKVKQIEESLK
                     H"
     gene            complement(11044..11400)
                     /locus_tag="PLS16_08065"
     CDS             complement(11044..11400)
                     /locus_tag="PLS16_08065"
                     /inference="COORDINATES: protein motif:HMM:NF017897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HNJ61074.1"
                     /translation="MEKKYKTLKAFYPYYLTEHSDPTCRVLHYIGTSLVILLLIWAIA
                     QPIWWKFVLLPLVGYSFAWIGHFKFEKNKPATFIYPLYSLASDFIMLYHFLIGKIDEK
                     LAEAKMIIAQENKNEQ"
BASE COUNT         4021 a         1580 c         1971 g         3865 t
ORIGIN      
        1 cgaaagaatc aatacatcaa acttcttcta attcacacga aaatcatggt catcatgatg
       61 attgttcgcc tttttgtagc tgtacttgtt gtacaacagg aactatagcc caatcaaact
      121 ataattaaaa agcaatcctt tctgattctt ttaattcata tacggatttg ataaaagttc
      181 catcaactga aatgaatttt atttctattt tttacggaga tatttggcaa ccgccgaaaa
      241 tttaattttt ttagaataaa ttattctgtt cacgtaaaga gcattttaaa tgctaattgt
      301 ttacataatt cagcttaatc tgaactataa aaattaaata aaatgttaga tagaattata
      361 ctatttagta taaaaaataa aataatcgta ggttttttta cactaatctt aattgtttgg
      421 ggaatttgga gtgctacaaa attacctatt gatgcaatac cagatattac caataatcaa
      481 gttcaaataa ttactgttac gccaacttta gccagtcagg aagtagaaca attaattact
      541 tttccaatag agcaaagtat cgcaaactta cctgatattg aagaaacaag aagtatttca
      601 cgttttggtc tgtctgtggt aactgttgta tttaaagata aggtagatat ttattttgca
      661 cgacaattaa taagtgagaa actgaaagaa gcacaagaag atattcctgc aggtttaggc
      721 aaacctgaaa tggctccagt cagtactggt ttaggtgaag tgtatcaata tattattcat
      781 cctaaaaaag gaagtgaaaa taaatattct tccaaagatt taagagaaat gcaagattgg
      841 attgttgcta gacatctgaa tggaacacca ggagttgctg aaattaatag ttttggtgga
      901 gaattaaaac aatatgaagt tgctataaat ccagatagat taattgcaat gaatgtgaat
      961 attagtgagg ttttttcagc actcgaaggt aataatcaaa atacaggagg ggcctatatt
     1021 gaaaaacaac ctaatgtttt ttttattaga ggtattggca ccgtcaattc attagatgat
     1081 atcggaaata taatgattaa aaatatcaat ggtgtgccgt catttattaa agatgttgct
     1141 gatgttcgat ttggacacgc tgttcgatat ggtgctttaa cgtataatgg cgaagtcgat
     1201 gcagttggtg gtgtggtcat gatgttgaag ggagaaaata gcaatgatgt tgtaaagcga
     1261 gtaaaagaaa aaatgcttgt tatacaaaaa tcgttacccg atgatgtggt gattgaggct
     1321 tatttagata gaacgaattt ggtaaataga gcaattcata ctgttgaaaa aaacttattg
     1381 gaaggtgcct tgattgttat ttttatattg gttgttttct taggaaattt tagagctggg
     1441 ttgatagttg catctgtaat tccattagct atgttatttg ctttaagttt aatgaatttg
     1501 tttggagtaa gtgccaattt aatgagttta ggggcgatag attttggttt aattgtagat
     1561 ggtgcagtga ttgtagttga agctactatg catcatttag gcctacgaaa atcagtacat
     1621 agattaactc aatcggaaat ggacgatgaa gtatttaatg cagcttctaa aattaggaaa
     1681 agtgcagctt ttggtgaaat aataattttg atagtctata ttccaattct ttctttagtt
     1741 ggaatagaag gaaaaatgtt cagaccaatg gcagaagcgg ttggttttgc catactagga
     1801 gccttaattc tttccttaac ttatgtaccg atgatgagtg ctttattcat gcccaaatca
     1861 actaatccta aagcaagttt tagtgataaa atgatggaga aattccaaca aatttatcaa
     1921 ccattattag aaaaagcgat tcaattaaag tatattatta ttacaatatc tatcatacta
     1981 tttagtataa gtattttgct ttttggaaga atgggaggtg agtttattcc aaatttgaaa
     2041 gaaggcgatt ttgcctttca ttgtatttta cctcaaggta cttcgctcaa tcaaagtata
     2101 gaaacatcta tgcaagcatc aagaattatt aaacaatttg atgaagtgaa aatggtagtt
     2161 ggaaaaacag gaactgcgga agtaccaacg gatccaatgc cttttgaaat tactgattta
     2221 attatagttt tgaaacctca aagtgaatgg aaaaataata aaacttatga agatttggca
     2281 gatgagatga tggaaaaatt agaagatttg ccaggtgttt tttttgagag tagccaaccc
     2341 attcaaatgc gttttaatga attaatgaca ggtgttcgac aagatgttgc tgtaaaaata
     2401 tttggtgaaa atatggacac tttaagtgct tatgcaaata gagttgcaag ggttatcaaa
     2461 aatatagatg gtgcaacagc accacaagta gaacgtgttg atggtttacc tcaaataaat
     2521 attgaatatg atagattacg tttggcaaat tatggcttgt caatacaaaa cataaatgat
     2581 attgtgagta tggcatttgc gggaaaaagc tcaggtatcg tatatgaaaa tgaaagacgt
     2641 tttgatttag ttgttcgatt agattctatt catagagata atattgataa cgtccgaaat
     2701 ttaatgattc caatttcatc aggtatgcaa attccacttt ctcaagttgc ggatattaac
     2761 tataaattgg caccagcaca aattagtaga gaagcaggaa agcgtagaat tgttgttggt
     2821 tttaatgtaa aagatagaga tgttcaaagt gtagtagagg atattcaaaa aactttagat
     2881 gaacaagtga aattacctac tggttattat tatacttacg gtgggacatt tgaaaatttg
     2941 caagctgcct cgaaaagatt aatgatagca gttcctttag ctttgttact aatttttgta
     3001 ttgttgtatt ttacatttca ttctgtaaag ttagcagttc taatttttac agcaattcca
     3061 atgagtgcta ttggtggtgt aattgcttta ttaattcgtg atatgccttt tagtgtgagt
     3121 gctggtattg gttttattgc tttatttggg gtggcggttt tgaatggtat tgttctaatt
     3181 ggaacattta atcaactaag aaaagaagga gaaacagata ttttgaaaat tgtagttgaa
     3241 ggtgcaaaaa taagattgcg tccagttttg atgaccgcat cagttgcaag tttaggattc
     3301 ttgccaatgg cactcagttc aagtgctgga gcagaagtac aaagaccatt agcaactgtg
     3361 gtaattgggg gtttaataac ggctacttta cttactttat ttgttttgcc tttattgtat
     3421 ttaattttta attctaaaga aagtatgcgt aataaaattc aatctaagtc tattgttatg
     3481 gtgttgttta ttttgttttt tggattttct ggaagcaatt tgtatgctca agctgaacgt
     3541 aaatcattag atgagctatt gcaattgtcg tcaaataatc tgcaatatca aattaataaa
     3601 actcaaaaaa ctcaaagtgc agcagcaata aaaggtgtgc aacgtattaa taaaacaggt
     3661 gtttttatag agaatgaaga cattcgacct agtgatagaa ttggtatttg gaaagttgga
     3721 ctttcacaag atattccttg gccaggtgtg tatcgctcac agaaaaaatt agctcaagaa
     3781 caatcaaaat tatttgatgt gggcgaacat ctaatttctg ctgaattaaa aagagacatt
     3841 acggcatctt attatgaaat gtggtatgtt caagataaaa tgaaattgta tgaaaagatg
     3901 gatagtattt attctacttt acatttggca acaaaactaa aagtaaaaac tggcgatagt
     3961 cctggattgg atagtgtttt ttctaaaatc aagatgaacg aaaattttgt gcttttaaaa
     4021 caactgaatc aagatattgc taaagtacaa aaacaattaa tgcttgagtt gaatactgac
     4081 caagtatatc ttccagaaaa taagccatta tctaaaattg aactaagctt tgataataca
     4141 ggtttacacc cagaattgca agcacaacaa caacaaattg ccattgctga aaaaactgta
     4201 aatgttgtta aacaagaaaa tttacctgaa atgagtactc gtgttttctc tcaaagatta
     4261 tatgggttaa aagatccata cactggattt tcagtaaata tagcttttcc aatatttgga
     4321 aatggtgcgt ataaaagtag attaaaagca gcacaaactt cagttcattt acaagagcaa
     4381 gaattagaat acaataaaca acaatttgat gtacaaaaag tacaattact tttagaagta
     4441 gaaaaaaata ctcagttatt agatttttat gaaactgaag gcttaaaaca ggctgaaaat
     4501 attattaatt ctgcactttt aagttaccaa actggcgata taagttttgc agatttgaat
     4561 gaatatttaa ctcaaacaat tcaaattaaa caacgatata ttgatgcttt gaatttatat
     4621 aatcaatctg taattcaata taattattac aacaacaaat aaaataaaga tgaaaattca
     4681 atttaaaata ctcccattta tattagcact tttaatgttt aatagttgca aaaataatca
     4741 cgaagatgga cacaatcatg aagcggaaac tagtgcagaa caaccaaata cggcagaaga
     4801 aagccactcg gttacagcat taactgatga gcaaataaaa gccgctggaa ttagttacgg
     4861 tactatagaa atgaaatcac taactgcagt tttacaagct aatggaaata taaaagttcc
     4921 taataataat aaagcaatga ttacttcctt atatggtggc gtaattaaat ctattcatgt
     4981 tgaatctgga aaaatcattg ctaaaggaca agttatagca accattgcaa accctcaatt
     5041 tattcaattg caagaagaat atttgactat caatagtaaa attacgtttg cagaacaaga
     5101 gttacaaaga caaacagaat tgagtgaggg caatgctgga gcaaaaaaga atttacaaag
     5161 tattacagca gaattgaatg gattgaaaat tagaaaagca tcactttttc aacaaattca
     5221 attaatggga attaatccaa gcacaataaa taattctaat ttaaaaacta ctataaatgt
     5281 actaagtcca ataaatggtg tcgtttccac attgtttgca aaaattggaa gttatgttga
     5341 tgttgctgct ccaattgctg agattattga taatcaagca atgcatgtgg atttgaatat
     5401 atttgaaaag gatatttcta atattaaatt aggtcaatca attattttta gaacgattaa
     5461 taatccagat aatcaatacg aagctgttgt acacactatc ggttcttctt ttgaaaatga
     5521 aactaaatct attgcagtac attgtgatgt gaaaggcaat cgaaaaggtt tgatagatgg
     5581 aatgagtgtt gtggcttcaa ttagtttaca taatatttta actgcttcag tgcctgatgc
     5641 agcgattgta aattttgaag gtaaagattt tatatttatc attacagaca aaataccaga
     5701 aatagaaaaa catgaagaca ctgatgaaca tggacatcaa catgaaccta aaaagactga
     5761 aaacgaaaat ataaaaacaa ctaattttga aagaatagaa gtgttaaaag gtgtttctaa
     5821 tatggggttt actgcaatta caccagttgt tacattacca gagaatgtaa aaattgctgt
     5881 gaaaggtacc ttttttatta atgctaaatt agtaaatact ggagaacatg aacattgatt
     5941 cattaattat ataagaaact tatggaacac caacatatat acgataaaga tggaaatcaa
     6001 ctgtgttgta caatggaaaa gaaaatctat ttgaatgcag gtgctcaaaa attattagga
     6061 aataattctg aacaacatga acacgaccac catcacgacc atagtggtaa attattgttt
     6121 ttattgcctt tgctttcatt agttttatta ctaactgcat tgtattttga ccatattcta
     6181 aaaccaattt ggtttaaaga aaccttccga ataatttggt atgttgcagc ttatcttcca
     6241 gttgggttgc ctgtcgtaaa agaatcgttg gaaagtattt taaaaaaaga tttcttttca
     6301 gaatttacct tgatggtaat tgcaaccatt ggtgcatttg ctattggaga atttcctgaa
     6361 gcagttactg taatgttgtt ttatgcaata ggagaggtgt ttcaaacttt ggcagtcaaa
     6421 cgagcaaaat taaatataaa aagtttatta gaccaaagac cagatgaggt tactatttta
     6481 gaaaataata ttgctaaagt cgtaaaagcg gaattggtag aaattgattc tgttatacaa
     6541 ttaaaacctg gcgaaaaact aggattagat ggagtattaa tctcagattc tgcatcattc
     6601 aacacagctg cattaactgg cgagagcaaa ccagatagca aacagaaagg cgaaagtgtg
     6661 ttagctggta tgataaattt gaatactgta actcaaattc gagtaacagc aaaatatgca
     6721 gatagtaaat taagtagaat attagaatta gtacaaaatg caagtgctca aaaagcacca
     6781 actgaattgt ttattagaaa atttgctaga atttatacac cgatagtggt taatttagcc
     6841 ttgctgatag tcgttttgcc ttatttattg catttaacaa acgtacaatt tttacctgat
     6901 agtattcaac attattcttt tcaaaattgg ctatacagag cgttggtttt cttagtaatt
     6961 tcttgtcctt gtgcattagt tatttctata cctttaggtt attttggtgg tatcggtgcg
     7021 gctagtcgtc atgggatttt atttaaagga agtaatttct tagatattat tgcagatatc
     7081 caacatgtag tgatggataa aacaggtaca ctaacggaag gcgtttttca ggtgcaggaa
     7141 gttgttttta atagtttatt tgataaggat aaaatattgc aatttgtcaa tgtaattgaa
     7201 agccatagta cacatccagt tgcaactgca attcttcaat atgttggcga aagaagtcct
     7261 gatattaaga tagataatat cgttgagatt tcaggacatg gtttaaaagg catggttgat
     7321 gataaagaat tattagtcgg gaattttaag ttaatggata agtttaatat tcaatataat
     7381 ttagacccga attcgataat ttataccttg attgctgtgg cttatgatgg tcaatttgtt
     7441 ggctatataa caattgcaga ttcaataaaa gcagatgcat caaagacgat tcaagctttg
     7501 catactatga atgtaaaaac tacaatgttg agtggagata agcatacagt ggtgcaatat
     7561 gttgcgaaag cattaaacat tgacaatgct tttggtgatt tattacctga agataaagtt
     7621 gaaaaagtaa aacaaataaa aaattcatat aagtcagttg catttgttgg agatggagtt
     7681 aatgatgcac ctgtagttgc attgagtgat gttgggattg caatgggtgg attaggtagt
     7741 gatgcaacta ttgaaacggc tgatgttgtc attcaagatg atatgcctag taaaattcca
     7801 atggctatta aaattggaaa agcaaccaaa aagattgtat ggcaaaatat tatcttggct
     7861 tttggtgtta aattgatagt gctgatttta ggagctggtg gtgttgcaac aatgtgggaa
     7921 gcggtttttg cagatgtagg tgtcgcatta ttagcaattt taaatgccat ccgaatacaa
     7981 agaatgaatt ttgataaata gtagagaagt gaatttttgt aagataatat cgacagatat
     8041 tggcatcata aatgaaattt atgcagtcta tacaagatca tttccagaag atgaaagacg
     8101 agatgaaaaa cagttttatg ctttgtttca aaatcctaaa gtacaagtgt ttttagtagt
     8161 ttttgagcat aatgctattg cttatttgat tacttggcag ttagaagaat ttgtatttat
     8221 tgaacatttt gaagtttttg aaacttatag aaatatgaaa tatggagcat tgattcttaa
     8281 tcaattttca gaaaaatttt ctaagataat tttagagtca gaacctgaaa ttatgggcga
     8341 actagccatt agaagacttg gattctatgg aagaaatggc ttttctattg ttgaaaggaa
     8401 ctatgaacag ccaccatatt caaaagaaaa gaacgccttg aaattgtatc tattatccaa
     8461 ttttaaaata gatgctatcg ctccaattgt actagaaatt tataaagttg tttatcaaaa
     8521 gaattttaat tatttgtact aatttgtttc tttctttaat gcattcagaa ataaatcaat
     8581 ttcttccttt tgaatagcta aactaggtaa aactctaagt gtattaggat tattagaatt
     8641 tccagtaaaa atttgatgtt tgaacaataa gttatttctt aaatctttaa taggttcttc
     8701 aaattctaaa cctaaaatca atcctttccc tctgatttgt tttaatcctt taataccctt
     8761 taaattctcc aataaatatt gtcccatttt ttgcgtattt tcaattaagt tttcattttc
     8821 aatgatatct aaaactgcta aagttgcggc acaagctaat ggtgaaccac caaaagtagt
     8881 acctagcatt ccataaactg cctgtatttt ttcgttaatt aaaacaccac ccactggaaa
     8941 accattgccc attcctttgg ctatggtaat tatatcaggt tgcacatttg caaattgatg
     9001 agcaaagaat ttaccagatc ttgcaaaacc acattgtact tcatctgcaa taaaaattgc
     9061 atcattttga tggcataatt cttgtatttt ttctaaaaaa gaagcacttg cttcatagat
     9121 accattcaca ccttgtatag gttcaacaat aacagcacaa atttcattgc cttgttcttt
     9181 aaatgttttt tctaaggcaa cattatcatt aaatggtaga aaaattacat ggtcatcagg
     9241 attgatggca ggttttattt ttggattgtc tgtaacagcc acagcagcag ccgttctgcc
     9301 atgaaatgct cctgaaaaag caatgacttt ttttctttta ttataaaacg aggctaattt
     9361 taatgcattt tcattggctt ctgctccact atttattaaa aataaattgt aattggcata
     9421 gccagaaatc tgtcctaatt tttgagctaa tttttgttgt atttctaaat gaacagaatt
     9481 tgaataaaaa accaactttt ctaattgttc attcaatgct ttgatataat atggatgagc
     9541 atgtccaaca gaaattaccg catgtccgcc ataaaaatct aaatattttt gcccattttt
     9601 gtcaaataca aatactcctt ttccgctttc taattctaca tctaaaagtg gatatacttt
     9661 aaataaatcc atataataaa atttatctcc gaaattccta aaaaaaatag agaaaatgat
     9721 gaattgtagg ttattaaatt gtagctaagt aagattgatt tttgattagc agtttacaat
     9781 ccaatgatgt gtatctgtat caataccttt ttgaatgttc actaattttt ctttgagttg
     9841 ttgagctact ttaaattgag tagcatctag tttgtaatct ttaccttgat agccaatttt
     9901 atctacagca ttaacaacta cagccgttcc tgttccgaat acttccgtca atgtgccatt
     9961 ttgatgagca gcaattacat cttctatatc aattaatttt tcttccactt cgtagcctaa
    10021 ttctttagca atagtaatga tactatttcg agttacacca gccaaaatgg agccatctaa
    10081 cattggagta accaatttat tattgataac aaagaaaata ttcatcgtgc caacctcttg
    10141 cacatattgt ttgtgttcgc catctaacca aagtacatct ttatagccat tttgtcttgc
    10201 aacttccaat ggatataatg aacctgcata atttccagca gctttagcat cgccataacc
    10261 accttttgct gcacggatat atttttcatg tacataaaca gaaataggtt cgccgtaata
    10321 agcaccaaca ggtccagtca taataataaa taaataacgg tctgaagttt ttacgccgat
    10381 gaatggctca gatgcaaaca tcactggacg aatgtataaa gctgaatttg gtacatttgg
    10441 tacaaatgct ttgtctagtt ctattaattt cttagttcct tcaataaata aatcttttgg
    10501 aatttctggc atagacattc tttttgcaga acggttcatt ctttcccaat tcatttcagg
    10561 acgaaacaaa acgacaccac tttcatctgc tttataaaat gctttcatac cttcaaaaat
    10621 ggcttgtcca taatgaaaaa cattacaagc aggagatagt tcaatgttgc cataaggttt
    10681 tatcacagca ctttgccaag ctccatcata atattcagca actagcatgt ggtcggaaaa
    10741 tgttctacca aattttatat cattaaaatc aatcgtatca attttactat gttgtgcttg
    10801 ttctattttt attggaaaca taaggtaata tttaataatg caaattaaat gaaattgtca
    10861 ttaatacagt aattaatagc tcggatttgt ctttaattta atgttttaaa gattcttcaa
    10921 tttgttttac tttcttgtct attgaaaata agtaaaataa cattccgata agaataatta
    10981 atattactgc aaaaactgca actaatttat ggttggataa aagtatttcg ttcatattat
    11041 ttattattgt tcatttttgt tttcttgtgc tataatcatt ttggcttcag ctaatttttc
    11101 gtcaattttt ccaattaaga agtggtatag cattatgaaa tctgaagcta aactgtataa
    11161 tggatagata aatgttgctg gtttattttt ttcaaatttg aaatgtccaa tccaagcaaa
    11221 actataacca accaatggca ataaaacaaa tttccaccaa attggctgtg caattgccca
    11281 aattaaaagt aaaatcacta aagatgttcc gatataatgc aacactctac acgttgggtc
    11341 ggagtgttca gttaaatagt acggataaaa cgcttttaaa gttttatatt tcttttccat
    11401 aatagttcaa agttaatgaa aaatagttta atcttaa
//
LOCUS       DAOQCW010000081        11349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_2153_length_11349_cov_4.849212, whole genome shotgun sequence.
ACCESSION   DAOQCW010000081 DAOQCW010000000
VERSION     DAOQCW010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11349
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2153_length_11349_cov_4.849212"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1536)
                     /locus_tag="PLS16_08070"
     CDS             complement(<1..1536)
                     /locus_tag="PLS16_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61075.1"
                     /translation="MLKYKKVFRVLKNINSQTFLITLFIGIIFPYYVEAQVRQAFTLR
                     GTTQSTPYYEAETKLFNLKGDFLQIGNSNVSTSKGNPNGENGNTRVTFNNIDNNTDVI
                     NSSAANIVFPETSCENEIVAAYLYWSGRATKRMIASTSNDQNRLGGYTLSRTTGGSGN
                     SGYVNFRFTGTNRTQYDFRLSRNGSLAYRIASSGAYITITPTNTYTTGSGSSQKRFVE
                     FLPIIMYDGNDYQIVISAVCREYSENQSSSIAYVDIIYKANDNLLKKNTVKIKAGNGA
                     YEDIIAETENSYYSETADNHMYYSSAEITNIVKNAYSIYGNNTTYTVANIYAGQSPDN
                     YSNDDGTGFYGGWGIVVIYHNPSKKLKDIAIFNGFAYVYNDAGYYDISLSGFRTVLNG
                     AVGVDIGVMAGEGDYAIDGDYLSIRQRNSNNFTKLSVPSNEGGNGNTNNYFHAYINGG
                     TGDNRGITRNPSIRNNTGLDISKISIPNNGNAIINNQQTQTTIRYGTDGDTYCIFNFV
                     FAAD"
     gene            complement(1669..2752)
                     /gene="prfB"
                     /locus_tag="PLS16_08075"
     CDS             complement(join(1669..2703,2705..2752))
                     /gene="prfB"
                     /locus_tag="PLS16_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793088.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNJ61076.1"
                     /translation="MTNEQLKSLLEKASALGGIFDVAHRIEKIKDDEAKTLTPNFWDN
                     QLLAQALMKEIKQDKHWVDIYQNVQTKLDDLIILKEFNEIGEATDEEVELQYNLVLAE
                     MEETEFKATLNKEEDVLSAILEINAGAGGTEACDWGSMLMRMYTMWAQKNDYKITELD
                     YQDGDVAGIKSVTLEITGDYAFGLLKGENGVHRLVRVSPFNAQGKRMTSFCSVFVHPV
                     VDDTIEIEINPADLEWDVFRAQGAGGQHVNKTESAVRVRHIPSGVVCECQTQRSQIQN
                     RETALKMLKSRLYEIELQKKLEARDALEAQKMKNEWGSQIRSYVLDDRWVKDLRSGYK
                     STNPDDVLDGNLNGFFKAYLLYKQEK"
     gene            complement(2844..3503)
                     /locus_tag="PLS16_08080"
     CDS             complement(2844..3503)
                     /locus_tag="PLS16_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61077.1"
                     /translation="MKYIFITISLFILNISYVLSKDNTHKNNSLGVNFYYNNHNQYFT
                     PGFMLDVNYEHVFNRWVGLQTSLGFNRAKVKLNDWKKDVLEQNYGYKYPSLTSSILLN
                     VVGNFYCINNLHSKLKLGFGIEYRNIADAITNSVVNLLPNQNDESTLYPNTHFEQFND
                     LGISGNISYQYLFNKGIGINTTFGYNHYFTNLSKKAKRGERLGGSNFLKLGIGVFYSF
                     K"
     gene            complement(3516..4676)
                     /locus_tag="PLS16_08085"
     CDS             complement(3516..4676)
                     /locus_tag="PLS16_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61078.1"
                     /translation="MTNSKNKTLSILIVIMIFIGCNKENKKTILYQEQKRITLNKEVA
                     KAMAIITTDKEIRQEIFASVSKITQMHQWRDEAIYFKEILPNNFNSILNKESKIAIAL
                     KTQLKKNRITIHTTSNKRNSEDISELINDLIEYEIEFYFPYHEDYPTPDNFAVTYQNE
                     ISEDENEGFIYVNNKQETILVNEVYATNNPTLIIGQFNNNRSETLGNSTSNQRTILDP
                     MYINIGRLKYTNDHEGLMMGGPEFTYYRAKGTINVNNNNKTVQSSDMVNIDFKRKDKG
                     KWHNINSMIDHSWNILEFNQVFGLYERDGGVKEMINLNINVETEIAGIRIPFGVNLNI
                     GSKDDEIGFNTYDRTMFFNTNKLNQNLGNGTEQSWPIYHMNGVFFTMPIINN"
     gene            5152..7278
                     /locus_tag="PLS16_08090"
     CDS             5152..7278
                     /locus_tag="PLS16_08090"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNJ61079.1"
                     /translation="MFHYSKDQDNIVTITMDMQGRSANVINAEFGNLIVDTLNKLEAE
                     KDNIDGVVLTSAKSTFFAGADIDNLYKQTDAKEIFDMCEGLKAQFRRLEKLGKPVVAA
                     LNGAALGGGLELALVCHYRVALNNPKAQFGLPEVGLGLLPGGGGIIRLTRMIGLQPAL
                     PLLTEGKRLKVKDALSNGLINEIAESPEDMIAKAKAWIKANPKAINPWDDKGFKFPGG
                     DAKNPKVASMIPIVPAMTKKKTHGNYPAVQAIINTAVEGSVVDFDTACRIESRYFTSL
                     ATGQVSKNMITAFWYNLNAINAGNSRPKGFEKYTTKKVGVLGAGMMGAGIAYVSAYAG
                     MEVVLKDVSIESAEKGKAYSEGILKKLISKGKVSKEKAEEILSRIKATDKPEFDGCDL
                     IIEAVFEDRNLKAKVTQEAEATMLTEGTFASNTSTLPITGLATASTRPKQFIGLHFFS
                     PVDKMPLVEIICGKETDDATLAKAFDYVLQIKKTPIVVNDSRGFYTSRVFSTYVMEGI
                     ALLAEGEHPRSIENAGLKAGMPVGPLALTDEVSLKLMDHIRQQTEKDLIAEGKEIPKH
                     PGLPVISKMLELNRPGKAAGAGFYEYPKDGKKHLWEGLRQQFPLKEQLSEHEMVERLL
                     FVQAIDTVRCVEENVLRSVADANIGSIFGWGFSPFNGGTLQYINGYGLDKFVKRAAEY
                     EAKYGERFAVPQLLKDKAAKGEIFVD"
     gene            complement(7310..>7836)
                     /locus_tag="PLS16_08095"
     CDS             complement(7310..>7836)
                     /locus_tag="PLS16_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61080.1"
                     /translation="FFGNTLGGFYTMGSKVIAAPSGLYQTALTQIFMQQAVHLSPTEL
                     HKMSKKILYFGFCLGILPSIIVLIFGGNIFSFLFGEEWQVAGNIASYIVLWYFVGALA
                     NPINFILDIYQKLDIELYWNTASFVVKFSCLLIGFYYHNFWLAIALLTIVSIMMNLIL
                     LVFSFRLLKQKRTA"
     assembly_gap    7837..7936
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7937..8691)
                     /locus_tag="PLS16_08100"
     CDS             complement(<7937..8691)
                     /locus_tag="PLS16_08100"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNJ61081.1"
                     /translation="MLRPLKKQYTIPNFYPIFGGLLLAQLFNLLNLLILPRFFSPEDF
                     ALFGIFSSLVFIIIEIVNLKLDIAIQVADETQRKHLTQIAIITAFFISLIVFIFSIVY
                     VLNYQHYYFLFLPLITFAYGINQSMLAWLNIHHKNHLISTYRVLIVIVNFVVSFCCIY
                     FWNLHFGLIFGFISAQSIAALFLLIINRSIFKSIISFDEMKNHFQVFSQFPKFGVLSS
                     LINTITQNGIIPSIVYFFGNTLGGFYTMGSKVIA"
     gene            8779..9357
                     /locus_tag="PLS16_08105"
     CDS             8779..9357
                     /locus_tag="PLS16_08105"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNJ61082.1"
                     /translation="MILWLFTLIFKLKGWKLKTEMIHQYPKSVTIAAPHTSNWDLIIS
                     LAAFNLMGLKVRFTLKEEWFRFPFKRIMIGFGGISIDRSPKQAGEKRLSIVEAMANLF
                     KENEKLHIMVTPEGTRSLRTEWKTGFYYTAKAAGVPIMCGYLDYKNKVAGIGKIVFPS
                     DNMEADLKEIMSFYKTIQGKNPEKFSTDLRYS"
     gene            9572..11047
                     /locus_tag="PLS16_08110"
     CDS             9572..11047
                     /locus_tag="PLS16_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNJ61083.1"
                     /translation="MKTATYKDEKKQVYSITEYHDVNPIYQKLENLVAQPLQHIQPDA
                     MKNYLAYYDTQCTKSKAIYEEAQKYIPGGVQHNLAFNYPFPLAFTKAEGAYLYDADGN
                     KYIDFLQAGGPTVLGSNYEYVREKAIKLLNECGPSTGLLHEYEVKLAKIICDNIPSVE
                     QFRMLGSGTEGVLGALRLARIKTGKTKIIKVGGAYHGWSDQMVYDLRIPGTKFFDAKG
                     IPFMMHWNTQAVPPNDIEALETTMKWNRLRGGTAAVIVEPLGPESGTYPLYRAYTQQV
                     RDLCDKYGALLIFDEVVTAFRVGMSGAQGYLGVMPDLTVFGKVVAGGYPSAGGLGGRA
                     DLMDGLAAGLQGGKKVKVGGTMAANPLSSCAGYYTLEEIKRTGACAIAGKAGDRMTKG
                     LNQLIDKYGVPFVAYNQGSICHLETAGTLFVKINFLKIKSVLGEINKRKKLMEEYGAA
                     YMAEGVVTLAGSRLYTSMADTDEVIDEALNKFENVFKNVQK"
     gene            11047..>11349
                     /locus_tag="PLS16_08115"
     CDS             11047..>11349
                     /locus_tag="PLS16_08115"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNJ61084.1"
                     /translation="MPYFFAYDVGTSSVKTILTDASGKIYGSAVVDYPLFMPNDGWVE
                     QEPEDYWKAICKATRQILSETKIDIQQIKGLAFTTQAMGVIPVDSHGNVLYRNITWV"
BASE COUNT         3853 a         1679 c         1829 g         3888 t
ORIGIN      
        1 atccgcagca aaaacaaaat taaagataca ataagtatcc ccgtctgttc catatcgaat
       61 ggttgtctgc gtttgctgat tatttataat ggcgttgcca ttattgggaa tacttatttt
      121 tgaaatatca agtcctgtat tatttcgaat actaggattc ctagtaattc ctctattatc
      181 tcctgtacct ccgttaatat aagcatgaaa atagttgtta gtatttccat ttcctccttc
      241 gtttgaagga actgaaagtt ttgtgaaatt attagaattt ctttgtctta tggataaata
      301 atcaccatcg atagcgtaat caccttctcc agccataaca ccaatatcta ccccaacagc
      361 accatttaag actgttctga aaccactaag cgaaatatca taatagcctg cgtcattata
      421 tacgtatgca aatccgttga atatagcgat gtcctttagt ttcttagaag gattgtgata
      481 gataacgact atgccccaac ctccataaaa tccagtgccg tcatcattac tgtaattgtc
      541 tggtgattga ccagcataaa tattagctac agtataagtc gtattattcc cataaatgct
      601 ataagcattc tttacaatgt ttgtaatttc agcagaagaa tagtacatgt ggttatctgc
      661 agtttctgag taatatgaat tttctgtttc agcaataata tcttcataag caccattgcc
      721 agcctttatc tttactgtat tcttttttaa aaggttgtca tttgctttat aaataatatc
      781 gacataagct attgatgaag attggttttc ggaatactct ctgcaaactg cactaattac
      841 tatttgataa tcgttaccat catacattat gattggtaaa aattcgacga atcgcttttg
      901 actactacca cttcctgttg tataggtatt tgtaggtgta atggttatat atgctcctga
      961 tgatgcaatt ctatatgcaa gtgagccatt gcgagatagc ctaaaatcat attgtgttct
     1021 gttggttcct gtaaatctga aatttacata gcctgagtta ccacttcctc cagttgttct
     1081 ggataatgta taaccaccta acctgttttg gtcattactt gtactagcta tcattctttt
     1141 tgttgcacga cctgaccaat ataaataggc tgcaacaatt tcattttcac aacttgtttc
     1201 tggaaaaact atgttagcag cggaagaatt tatgacatct gtattgttgt caatattatt
     1261 aaatgttact cttgtatttc cattttctcc atttggatta cctttagatg tagaaacatt
     1321 actattacca atttgtaaga aatctccttt tagattaaat agcttagttt cagcttcata
     1381 ataaggcgta ctttgagtag tgcctcttaa agtaaaagct tgtcttactt gtgcttccac
     1441 atagtaagga aatattatac cgataaatag ggtaattaaa aaggtttggg aatttatgtt
     1501 tttaagtacc ctaaatactt ttttatattt taacataaaa aaaagtcttt attaacacaa
     1561 acttaaacta tattttacaa tatagcaaat tttatacgaa tttttaatct aaatcaataa
     1621 aagtcatgtt caaataatat atcaagaaaa taaatctctt ttataagttt atttctcttg
     1681 tttatacaat aaataggctt tgaaaaagcc attcaaattt ccatctaaaa catcatctgg
     1741 gttagtagat ttataaccac ttctcaaatc ttttacccat ctatcatcta atacgtaact
     1801 tcttatttgt gaaccccatt catttttcat tttctgagct tctaatgcat cacgagcttc
     1861 taattttttc tgtaattcaa tctcgtacaa cctagatttt agcatcttta gagctgtttc
     1921 tctattttgt atttgtgaac gttgtgtttg gcattcgcaa acaacgccgc ttggtatatg
     1981 gcgtacacga accgccgatt ctgttttatt tacgtgctgt cctccagctc cttgagctct
     2041 aaaaacatcc cattccaaat cagctgggtt gatttcaatt tctattgtat catcaactac
     2101 aggatgcaca aaaacagaac aaaatgaagt cattcgtttg ccttgtgcat tgaatggaga
     2161 aacacgtact aaacgatgta caccattctc acctttaagt aaaccaaaag catagtcacc
     2221 agttatttct aatgtaaccg attttatacc ggcaacatct ccatcttgat agtctaattc
     2281 agtgatttta taatcatttt tttgagccca catcgtgtac atacgcatca acatcgaacc
     2341 ccaatcgcag gcttccgttc cacctgctcc agcattaatt tctaaaattg cagatagtac
     2401 atcttcttct ttgtttagtg ttgctttaaa ttcagtttct tccatttcag caagtaccaa
     2461 attatattgc agttccactt cctcatcagt agcttcgcct atttcattaa actcttttaa
     2521 aataattaaa tcgtctaatt ttgtttgtac attttgataa atatccaccc aatgcttgtc
     2581 ttgtttgatt tctttcatca atgcttgagc aagtaattgg ttgtcccaaa aattgggagt
     2641 taaagtttta gcttcgtcgt cttttatttt ctcaattctg tgagcgacgt caaagatacc
     2701 tccccagcgc cgacgctttt tccagtaagg attttaattg ttcattagtc atagtacaaa
     2761 aatacatttt tttctttaga tgaaaaagaa gtaatagcac caattttcgg agttatttct
     2821 tggtagtttt tcttttgatg aatttatttg aaggaataaa acacaccaat gcctaatttc
     2881 aaaaagttac tgccacctaa tctttcacct cttttggctt tcttgcttaa atttgtaaaa
     2941 taatgattgt atccaaaagt agtattgata ccgataccct tattaaataa atattgataa
     3001 cttatattcc cagaaatacc caaatcatta aattgctcaa aatgtgtgtt tgggtataaa
     3061 gtactttcat cattttggtt gggtaacaaa tttacaacgc tattagttat tgcatcagca
     3121 atatttctgt attctattcc gaagcctaac tttagtttgc tgtgcaaatt attgatacaa
     3181 taaaaatttc caaccacatt tagcaaaatg cttgatgtta aacttggata tttgtaacca
     3241 taattttgtt caagtacatc ttttttccaa tcattcaatt taactttagc acgattaaag
     3301 cccaaagatg tttgtaaacc tacccaacga ttaaaaacat gttcgtaatt tacatcaagc
     3361 ataaaccctg gtgtaaaata ttgattgtgg ttattataat agaaatttac acctagacta
     3421 ttgtttttat gagtgttgtc ttttgaaaga acataactga tgtttaatat aaataatgat
     3481 attgtaataa atatatattt cattttatgt attatttaat tgttaataat tggcatagta
     3541 aaaaacacac cattcatatg ataaataggc caactttgtt ctgttccatt ccctaaattc
     3601 tgatttaatt tattcgtatt aaaaaacata gttctatcat aagtattaaa ccctatttca
     3661 tcatcttttg atccaatatt taaattgact ccaaatggaa ttcttattcc agctatttct
     3721 gtttctacat taatatttaa atttatcatt tcttttacac ctccgtctct ttcataaagt
     3781 ccaaatactt gattaaattc taagatattc catgaatggt ctatcattga atttatatta
     3841 tgccatttcc ctttatcttt tcttttaaaa tcaatattaa ccatatcact agattgaaca
     3901 gttttattat tattgtttac atttatagtt cctttagctc tgtaataggt aaattcagga
     3961 ccacccatca ttaagccttc atggtcattt gtatatttaa gtcttccaat attaatatac
     4021 atcggatcta atatagttct ttggttagaa gtactatttc ctaaagtttc acttctattg
     4081 ttgttgaatt gaccgattat taatgtcgga ttatttgttg cataaacctc atttacaaga
     4141 atcgtttctt gcttattatt gacataaata aatccttcat tttcatcttc actaatttca
     4201 ttttgataag ttactgcaaa attatcaggt gtaggataat cttcgtgata tggaaaataa
     4261 aattctatct catactctat taaatcattt attaattcag aaatatcttc actatttctt
     4321 ttattcgaag tagtatgaat tgtaattcta tttttcttta attgagtttt taatgcaata
     4381 gcaattttac tttctttgtt taatatacta ttaaaattat taggtagtat ttctttaaag
     4441 tatatggctt catctcgcca ctgatgcatt tgagttattt tacttacaga tgcaaatatt
     4501 tcttgtctta tttccttatc agttgttata attgccatag cctttgcaac ttctttattt
     4561 aaagtaattc ttttttgttc ttgatataat attgtttttt tattttcttt attgcaacct
     4621 ataaatatca taattactat taatattgaa agagttttat ttttagaatt tgtcattttt
     4681 atttttttag ctttaaataa tgaattaaat tttagttcta ttatttaaaa ctctaaggag
     4741 gttctgaaat gcttttctga aaaataatat tgattacata tatatatgta tgtatgtatg
     4801 tatgtatgta tgtatgtatg tatgtccata atttttcgaa aattacaata tttatttata
     4861 atatcacaat attttagtat tttgtaatta aaatcatatc aatatttttc ttagccaaag
     4921 tcaagtgtta taacatgtcc tatcactaat tctgttgaca gatccataca cctgctaaag
     4981 acgtagaatt atttttctct acatcctaat ttcttgaata atatttactt taacttaaca
     5041 aattatccaa cgtttaaaaa aaataaaatt agttttccta tattggagac ttatttaaaa
     5101 tataatttaa ctaaacgtaa gaaaaatatt aactaaaaaa caaattaaaa tatgttccac
     5161 tactcaaaag accaagacaa tatcgttaca ataacgatgg atatgcaagg caggagtgcc
     5221 aatgtaatta acgctgaatt tggaaacctc attgttgata ctttaaataa gctagaagct
     5281 gaaaaagaca atatcgatgg tgttgtatta acttctgcaa aatctacatt ctttgcaggt
     5341 gccgatattg ataatcttta taagcaaaca gatgcaaaag aaatttttga tatgtgtgaa
     5401 ggattaaaag cacaattcag aagattagaa aaattaggaa aaccagttgt cgctgcctta
     5461 aatggagctg cattaggtgg tggcttagaa ttggcattgg tttgtcatta tagagttgct
     5521 cttaataatc ctaaagctca attcggatta ccagaagttg gattaggttt attgcctgga
     5581 ggtggtggta ttattcgtct aactagaatg ataggtttgc aacctgcttt accattattg
     5641 acagaaggaa aaagattaaa agttaaagat gctttaagca atggattgat taatgagatt
     5701 gcagaatctc ctgaagatat gatagcaaaa gcaaaagcgt ggataaaagc aaatcctaag
     5761 gctataaatc cttgggatga taaaggcttt aaattccctg gtggagacgc taaaaatcca
     5821 aaagtagcca gtatgatacc gattgtgcca gctatgacaa aaaagaaaac acacggaaat
     5881 tatccagctg tacaagcaat tattaatact gccgttgagg gttctgttgt agatttcgat
     5941 acagcatgta gaattgaatc cagatatttt acctccttag caacaggaca agtttctaaa
     6001 aatatgatta ccgctttttg gtataatttg aatgctatca acgctggaaa ttctagacct
     6061 aaaggatttg aaaaatatac tactaaaaaa gttggagttt taggtgctgg aatgatgggt
     6121 gctgggattg cttatgtatc agcctatgca ggaatggaag ttgttttaaa agacgtaagt
     6181 atagaatcag cagaaaaagg aaaagcttac tcagaaggta tattgaaaaa attaatttca
     6241 aaaggaaaag tatctaaaga gaaagcagag gaaattttat caagaattaa agctactgat
     6301 aaaccagaat ttgatggttg cgatttaatt atcgaagcgg tttttgaaga tagaaatcta
     6361 aaagcgaaag ttactcaaga ggctgaagca actatgttga cagaaggaac atttgcatca
     6421 aatacttcta cattaccaat tacaggttta gcaacagcat caacaagacc aaaacaattt
     6481 ataggcttgc actttttctc tcctgtagat aaaatgcctt tggtagaaat tatctgcggt
     6541 aaagaaaccg atgatgcaac tttggcaaaa gcatttgatt atgtattaca aattaagaaa
     6601 acgccaattg tggtaaatga tagtcgtggt ttctatactt caagagtttt ctctacctat
     6661 gtaatggaag gaatagcctt attggcagaa ggagaacacc cacggtctat tgaaaatgca
     6721 ggtttaaaag caggaatgcc agttggccca ttagctttaa ctgatgaggt tagcttgaaa
     6781 ttaatggatc atattcgtca acaaactgaa aaagatttaa ttgcagaagg taaagaaatt
     6841 cctaagcatc ctggtttgcc tgtgatttca aaaatgttag agttaaatcg tcctggaaaa
     6901 gctgcaggtg ctggatttta tgaatatcca aaagacggca aaaaacattt atgggaaggc
     6961 ttgagacaac aatttccatt aaaagagcaa ttatctgaac atgaaatggt agaacgtttg
     7021 ttatttgttc aagcaattga tacagttcgt tgtgttgaag aaaatgtatt aagaagtgtt
     7081 gctgatgcaa atattggttc tatatttgga tggggcttct ctccatttaa tggtggaaca
     7141 ttgcaatata ttaatggata tggtttagat aaatttgtaa aacgtgcggc tgaatatgaa
     7201 gctaaatatg gcgaaagatt tgcagtacct cagttgttaa aagataaagc cgctaaaggc
     7261 gaaatatttg tagattaatt caagtaattt attagaaaaa aggacagcgt taagctgtcc
     7321 ttttttgttt taataatcta aaagaaaata ccaataaaat taaattcatc ataattgaaa
     7381 caatagtgag caaagcaatg gctagccaaa agttatgata atagaagcct atcagtaaac
     7441 aagagaattt tactacaaat gaggcagtat tccaatataa ttcaatatcc aatttttgat
     7501 aaatatctaa aataaaattt attggattgg ctaacgcacc aacaaaatac cataagacaa
     7561 tatacgatgc aatatttcca gcaacttgcc attcctctcc aaatagaaag ctaaaaatat
     7621 ttccgccaaa tattaaaaca ataatactcg gcaaaatacc taaacaaaat ccaaaatata
     7681 aaatcttttt actcatttta tggagttcag ttggagataa atgaacagct tgttgcatga
     7741 aaatttgtgt caatgcagtt tggtataatc cactgggtgc tgcaataacc tttgaaccca
     7801 tcgtataaaa tcctcctaaa gtattgccaa aaaaatnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnngcaa taacctttga acccatcgta taaaatcctc ctaaagtatt
     7981 gccaaaaaaa taaacaatac ttggaataat tccgttttga gttatcgtat ttatcaaaga
     8041 agatagaaca ccaaattttg gaaattgact aaatacttga aaatgatttt tcatttcatc
     8101 aaaagaaatg atagacttga atatagaacg attgataata agcaaaaata gagcagcaat
     8161 tgattgtgcg gaaataaatc caaatatcaa accgaaatgt aaattccaaa agtatataca
     8221 acaaaatgaa accacaaaat tgacgattac aattaatact ctataggtag aaatcaaatg
     8281 atttttgtga tgaatattta accatgctaa catgctttgg ttgattccat aagcgaatgt
     8341 tattaatggt aaaaatagaa aataataatg ctgataattt aatacatata ctattgaaaa
     8401 gatgaataca atcaatgaaa taaagaacgc agtaataata gctatctgtg ttaagtgttt
     8461 tctttgggtt tcgtcagcaa cttgaatggc aatatctaat ttaagattga ctatctctat
     8521 aattataaat actaatgaac taaaaatgcc aaaaagtgca aagtcctcag gggaaaaaaa
     8581 tctgggtaaa atgagtaagt tgagtaagtt aaaaagttgt gccaaaagca atccaccaaa
     8641 tatcggatag aaatttggaa tggtatattg cttttttaat ggccttaaca tttgtcaaaa
     8701 ataagacata tttaaagtat ttctgaactg aaaaattgga attgtttcaa aagtcgtata
     8761 tttggaaaaa attgagttat gattctttgg ctttttactt taattttcaa attaaaaggt
     8821 tggaagttaa aaacggagat gatacatcaa tatccaaaat cagtaacaat tgcagctcca
     8881 cataccagca attgggattt aattatttct ttagctgctt ttaatctaat gggtttaaaa
     8941 gttagattta cattaaaaga agaatggttt aggtttccat ttaaaagaat aatgattggt
     9001 tttggaggta tttctataga tagaagccca aaacaagcag gcgaaaaaag actgagtatt
     9061 gtagaagcaa tggctaattt atttaaagaa aatgaaaaat tgcatattat ggttactcca
     9121 gaaggcactc gctccttgag aactgaatgg aaaactggtt tttattatac tgcaaaagct
     9181 gctggtgttc caattatgtg tggctactta gattataaaa ataaagttgc aggtatcgga
     9241 aaaatcgttt ttccttccga taatatggaa gctgatttaa aagaaattat gagtttttat
     9301 aaaaccattc aaggtaaaaa tccagaaaaa ttcagtacgg atttgagata ttcataagac
     9361 ttgtaattcc tatttctaaa ggagttttat gcttttatta aagtatattt agtgtttata
     9421 atattcataa tataatttgt tattgtaatt aatattttta taatcaattt attgatagaa
     9481 agtatcttgt ttttgttttt tgataattat caaaaaatag tatgtgtttt ttttactaac
     9541 tttgttaagc taatcaaaca atagatttaa tatgaagact gcaacttata aggatgaaaa
     9601 aaagcaagta tattctatca cagaatatca tgatgttaat cctatctacc aaaaattaga
     9661 aaatttagta gctcagccat tgcaacacat acaacctgat gcaatgaaaa attatttagc
     9721 ttattacgat actcaatgta caaaatctaa agcaatttac gaggaagctc aaaagtatat
     9781 tccaggtggt gtacagcaca atttagcatt taattatcct ttccctttag catttacaaa
     9841 agctgaaggt gcttatttgt atgatgctga tggcaataaa tatatagatt ttttacaagc
     9901 tggtggtccg actgttttag gtagcaatta tgagtatgta agagaaaagg caattaagct
     9961 tttaaatgaa tgtggtccat caacaggatt attacacgaa tatgaagtga aattggcaaa
    10021 aattatttgc gataatatac catcagtgga gcaattcaga atgttaggta gtggaacaga
    10081 aggcgtttta ggtgcattgc gtttagcaag aatcaaaaca ggaaaaacta aaattattaa
    10141 agtaggtggt gcttatcatg gttggagtga tcagatggtt tatgatttga gaatcccagg
    10201 tactaaattc tttgatgcca aaggtattcc ttttatgatg cattggaaca cacaagcagt
    10261 tccaccaaat gatatcgaag ctttagaaac tacaatgaaa tggaatagat tgcgtggcgg
    10321 tactgctgct gtaattgttg agccattagg tcctgaaagt ggtacttatc ctttatatag
    10381 agcatacacg cagcaagttc gagatttatg cgataaatat ggtgctttat taatctttga
    10441 tgaggttgtt actgcattta gagtgggtat gagcggagct caaggatatt tgggagtaat
    10501 gcctgattta acagtgttcg gaaaagtagt agctggcggt tatccatctg ctggtggttt
    10561 aggtggaaga gctgatttaa tggacggttt agctgctgga ttacaaggtg gcaaaaaagt
    10621 aaaagtaggc ggtactatgg ctgcaaatcc attaagtagt tgtgctggat attatacatt
    10681 agaagaaatt aaaagaactg gggcttgtgc tatcgcaggg aaagctggcg atagaatgac
    10741 caaaggttta aatcaattaa ttgataaata tggtgtgcca tttgtagcct acaatcaagg
    10801 ttcaatttgc catttggaaa cagctggaac tttatttgta aagattaatt tcttgaaaat
    10861 taaatctgta ttaggtgaaa ttaataagcg aaaaaaattg atggaagaat atggtgcagc
    10921 atatatggca gaaggtgttg ttacattagc aggaagcaga ttatatacaa gtatggcaga
    10981 taccgatgag gtaattgatg aggctttgaa taagtttgaa aatgtgttta aaaacgttca
    11041 gaaataatgc cgtatttttt tgcttatgat gttggaacga gtagtgtaaa gacaatcctg
    11101 acggatgctt ctggaaaaat ttatggaagt gccgtagtag attatcctct ttttatgcca
    11161 aatgatggtt gggtagaaca agagcctgaa gattattgga aagctatttg caaagctact
    11221 cgacaaattc taagtgaaac aaagattgat attcagcaga ttaaaggact agcttttact
    11281 actcaagcga tgggtgtaat tccagtggat tcgcatggaa atgtattgta tagaaatatt
    11341 acttgggtg
//
LOCUS       DAOQCW010000082        11191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_2181_length_11191_cov_5.287177, whole genome shotgun sequence.
ACCESSION   DAOQCW010000082 DAOQCW010000000
VERSION     DAOQCW010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11191
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2181_length_11191_cov_5.287177"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PLS16_08120"
     CDS             complement(<1..384)
                     /locus_tag="PLS16_08120"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HNJ61085.1"
                     /translation="MNKIFIYLLISITFFTISCKKESKQQYGDVVANITDNVIVETYD
                     DMEDGADDLLDAAKELKDDQNNATKLEEAKNAWIATRAAWEASEGFLFGPVSDLEIDP
                     NIDTWPLDSTGINAILAGTDVINKAY"
     gene            complement(456..2645)
                     /locus_tag="PLS16_08125"
     CDS             complement(456..2645)
                     /locus_tag="PLS16_08125"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNJ61086.1"
                     /translation="MKRKIKIYCLLILLCFANSLNAQDIDSLLQYYDSIQKTVIDEVT
                     IEAERYKEIRLKSIEGTIIYEGKKTEIIDLSKLQANTATNNGRQVFGRIAGVNVWEND
                     AAGMQLGIGARGLNPNRSAEFNIRQNGYYITADAIGYPDAYFTPPIEALDRIEVVRGA
                     ASLQFGTQFGGLVNFRTHHPIDKKFQFTSQTTAGSFGTVSVFNAIEGTSKNLTYYAYY
                     QYKRTKGWRPNSNLNAHTGFIQLGYQINPKLKISLEFTHQNYLMQQPGGLTDNQYKQD
                     AQQSTRTRNWFSIDWNMPSANIEYSPKSNLKWNTVLFGLIGSRNALGFLSYTNRADDF
                     RERDLIKDKYKNFGIESRVLYQYKLGKEQSSLSIGTTYFKGFTMKQQGLGSANSDADF
                     KYLNPLDLENSDYQFKSNNLALFAENIFKISSKFSITPGIRAEFLFTDVNGYYKLDST
                     NYNDSRQRNRMFPLLGVGISYKPHNSIEIYSNVSQNYRGINYTDLRINNPNVRIDPNL
                     HDEKGFNADIGIRGKKGNKFNYDANFFFLFYNKKIGSIQKVDSLFNIYRYKTNLSNSR
                     SLGIESFIEVDIVKCINENSPFGFSLFSNLTYTNAVYLKSQNKQIEGKKVEFAPQWIF
                     RGGIGFKYKTLALAWQVSTLSQQFTDATNAKYASTAIIGTIPSYYVMDLSASYSYKFL
                     KLSCSINNMSNNTYFTRRAESYPGPGIIPGDGIGVFGTITFKLNTKN"
     gene            complement(2629..3984)
                     /locus_tag="PLS16_08130"
     CDS             complement(2629..3984)
                     /locus_tag="PLS16_08130"
                     /inference="COORDINATES: protein motif:HMM:NF016946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HNJ61087.1"
                     /translation="MVINSETSNSIKNLLFKQVSIAPLASFRILFGAMILFSSIRFVA
                     KGWVDLLYIQPSYHFGYEGFLWLHPLSGIGMYIVYGIMIISALGILLGAFYRIATLLF
                     FLTFTYVELLDKTNYLNHYYFISIASLLMCILPANKYASLDVKLGITSKANFIPNWCI
                     LAIQLQIGIVYFFAGIAKINPDWLLEAQPLKIWLKSRDYLPIIGKLFEYDITAYLFSW
                     FGMLFDISIFFFLLNKRTRPYAYAAVVFFHICTGYLFQIGVFPYVMIVATWIFFSADF
                     HQRFLSIFINYKSNENIYYQPKFNRVWVTLLLIHFSIQILLPFRYLLYPGHLFWTEQG
                     YRFSWRVMLMEKNATTEFIIKDKNGKIEAVRNRDFLTPHQEKQMSTQPDMIIQFAHFL
                     ANEYNKNKGFEDAAVYVESYAALNGHPSRPFINEKINLAKEPINLLNKKWILPYDEKK
                     N"
     gene            complement(3974..5083)
                     /locus_tag="PLS16_08135"
     CDS             complement(3974..5083)
                     /locus_tag="PLS16_08135"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HNJ61088.1"
                     /translation="MKYNKLFLFSIILILVTCNKKSSDDDNFGKKEMFTNYAENLIIP
                     AYQNYGTKLSDLQAAFDAFKTAPDITKLNTLQNAFSATYEAWQYCEIYDKTAPADAVM
                     STENSNYYPCSADSIEAYITRGDNSVASIKSKRKYYKGLAAIEYLLFSRTLTNQEILD
                     RYTTSANANSYKTYLGSLITNLQTIQSTINTSWSNYSSIFIENVSTDASSAFSTMVNS
                     IAQRTDDLKRMQVGKPAGYQGNVSTIYTAPNAVQAYYSDHSIDYMLLTLQNMKDVLNG
                     KAALDGKGIIDYVRTLGYSSTFGGNLADDILNQIDVCIAKVNDCGADYSVTVSSNKPK
                     ADALFLETKKLLVLIKVDLPSALGVSINYTDSDGD"
     gene            complement(5268..6062)
                     /locus_tag="PLS16_08140"
     CDS             complement(5268..6062)
                     /locus_tag="PLS16_08140"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HNJ61089.1"
                     /translation="MWKISGNNLKQNSYLFGTIHAIPIDDYFIGKNTIKKFKECTNLV
                     MEMDLVQLDEMKIANLSLLPEDKTILDYLSPKEYDDLENFLINQFGMSKAMFENVYSR
                     LKPFFVDQFIMMSIIGDNKKVYEEELNLLANDYHLTKTGLETIEEQLQAIENIPLEIQ
                     YKNLIKGIDDYEAQKSNYTKMVSAYKKQDLNFLNEQFESEFSDDMRIYKTTLLDQRNK
                     KWVDKIIPMIEKEACFIAVGAGHLSGDSGLIKLLQQLGYQVTPEKK"
     gene            complement(6291..6998)
                     /gene="kdsB"
                     /locus_tag="PLS16_08145"
     CDS             complement(6291..6998)
                     /gene="kdsB"
                     /locus_tag="PLS16_08145"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNJ61090.1"
                     /translation="MKILGIIPARFGSLRFPGKPLVNINGKTMIQRVYEQTCLANELD
                     KVIVATDSLYIYQNIKNIGGLALLTNSEHLNGTSRCAEAFNLLDEPYEYIINIQGDEP
                     FINPIQIDELAIFLKLHKPEISTQIKKEFDLSLLDNPNIVKAILTNEIKAKTFTRKNI
                     KNNQDFFYKHIGLYAFRSDILNKIVQLSPSKNELNENLEQLRWLDNNYKIAVQETTFQ
                     SKSIDTLEDLDIIILKN"
     gene            complement(6995..8329)
                     /locus_tag="PLS16_08150"
     CDS             complement(6995..8329)
                     /locus_tag="PLS16_08150"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNJ61091.1"
                     /translation="MKNKILATLLLLCSISYAKDNKIYYLKLHAEIDKAAARLIHKGI
                     EEAEKQKATFFVLDLNTFGGLLDAADEIRSTLLNTKLKTVVWINNNAASAGALISIAC
                     DSIFMIEGANIGAATVVNQSGEKAPDKYQSYMRSLMRSTAETNGRNPKIAEAMVDEDV
                     QVPGIKDTGKILTFTTQEALKNHYCDAILNSKESVFKHISPEPYVVISHVETTIDKII
                     SFLLNPFINSLLIMMIIGGIYFELKMPGIGLPTLVAIIGAIAYFAPLYLEGLAANWEI
                     LLFVIGLILILLEIFVIPGFGVAGVLGIILIIAGLVLSLVNNQHFGLPGGIFEAIGMV
                     IFRVTVTILIAFILLFTVGGSIFNFPILNKMVLTTDQKSTEGFSIKQENHSDLVGKTG
                     IAITDLRPSGKVEIDDNIYDAITDAAFIVQGETIEVIKGEGYSIKVKKAKFS"
     gene            8501..9382
                     /locus_tag="PLS16_08155"
     CDS             8501..9382
                     /locus_tag="PLS16_08155"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNJ61092.1"
                     /translation="MEKGETLYGLSKKYKTTVDAIYKNNPALTSDNLKLGTELRIPQP
                     KELIKKPTTPTTTPLPSVSDNKKVEIKSTMIAKAKIDTILHQIQAKETLYGLSKKYNV
                     SIDDIRKWNKLSDAGIQIGMILKILKPASISINPNEKVEVEKKNEAIQPTVVEKVVVL
                     PEKQTPIKIDTEKVEPPKEVEKPVAPKTLSLYELFANQPKSALKKIKATGAPMNTTDE
                     SIDNTFFALHKTAQLGSVIKVKNLENNKVSYAKVIGKLPDTDENRKVLVRLSLGVRNA
                     IKMNNGKAYLEMEFINE"
     gene            9455..10486
                     /gene="hemH"
                     /locus_tag="PLS16_08160"
     CDS             9455..10486
                     /gene="hemH"
                     /locus_tag="PLS16_08160"
                     /inference="COORDINATES: protein motif:HMM:NF012964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNJ61093.1"
                     /translation="MGKIGVLLVNLGTPDSPSNSDVYKYLIEFLTDKRVMDFGWLKRN
                     LLVRGIIVPARYKHSAKTYRAIWDKDRGSPLLFHTEDLGKKVQSILGDKYVVEIAMRY
                     QNPSIESALKKLQAQLVSEIIVLPLFPQYSSACNGSIIQKVQEITNKWLTLPKLTYIN
                     HFYDNKLFIDAIVENARQFNLNDYDHIIFSYHGLPLRHLLNGDITGLYCKADKTCCNT
                     ITEVNQNCYRTQCFQTTKLVAEQLGLSSDKYTNTFQSRLGKEIWIEPYSVDVIPALAK
                     QGKRKLLVFSPAFVADCLETLFEISVEYQELFEEHGGHSVQLVPSLNSNQTWVNAVAD
                     IVMKNQLKK"
     gene            complement(10500..11159)
                     /locus_tag="PLS16_08165"
     CDS             complement(10500..11159)
                     /locus_tag="PLS16_08165"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNJ61094.1"
                     /translation="MYKFITIEGNIGAGKTTLASLLSEEYGGQLILEAFSDNPYLSKF
                     YQQPEKYGLQLEMSFLIERYQQLTKIFAEPNIFSQFIITDYMLKKCLLFAKINLSKQE
                     FKLYHSFFNLIYKKLPKPDIIFYLHSDTETLLRNIKRRGRDYEQSISKVYLNKIERMY
                     FEYFRQNPENKFVIVDINGVDWISNVFAYEKLKEVFSKEYNVGMNFIKLHNDNLLELP
                     L"
BASE COUNT         3917 a         1707 c         1627 g         3940 t
ORIGIN      
        1 atatgctttg tttattacat cagttcctgc taaaatagca ttgattccag tactatctaa
       61 aggccaagta tcaatatttg gatcaatttc taaatcagaa actggaccaa ataaaaagcc
      121 ttcactagct tcccaagctg cacgtgttgc aatccaagca ttttttgctt cttcaagctt
      181 tgtcgcattg ttttggtcgt cttttaattc tttagcagca tctaataaat catcagcacc
      241 gtcttccatg tcatcatagg tttctactat tacattatct gtaatatttg caacaacatc
      301 accatattgt tgtttgcttt cttttttaca actaatagta aaaaaagtaa ttgaaattaa
      361 taagtaaata aatattttgt tcattatata gaattttata caaaattaaa ttaatctaaa
      421 taaaaacaaa tacctaatta ttgtaaatta tatacctaat ttttggtatt tagcttaaaa
      481 gttatagttc caaatacacc gattccatct ccaggaatta ttccgggacc aggatagctt
      541 tctgctcttc ttgtaaaata ggtattatta ctcatattat ttatagaaca acttaatttc
      601 aaaaacttat aagaatagga tgcggataaa tccattacat aataagaagg gatagttcca
      661 ataatggcag tcgatgcata ttttgcattt gtagcatctg taaattgctg acttaatgta
      721 gatacttgcc aagctaatgc aagcgtttta tatttaaaac caatgccacc tctaaaaatc
      781 cattgtggtg caaattcaac ttttttacct tcaatttgtt tgttctgtga tttaagatat
      841 acagcattcg tataagtcaa attagaaaat aaactgaaac caaaaggact attttcgtta
      901 atacatttta caatatcaac ttctataaat gattcaatac ctaagcttct tgaatttgaa
      961 agattggttt tgtatctgta aatattaaac aaactatcta ctttttgaat agagccaatt
     1021 tttttgttat aaaataaaaa gaagaaatta gcatcgtaat taaatttatt gccttttttt
     1081 cctctaatgc ctatatctgc attaaaacct ttttcatcat gtaagtttgg atcgatacga
     1141 acatttggat tattaatacg taaatcggta taattaatac cacgatagtt ttgagaaaca
     1201 ttactgtaaa tttctatact attatgaggt ttataggaaa tgcccacacc taataacgga
     1261 aacatacgat tacgttgtct tgaatcgtta taatttgttg aatctaattt ataatagcca
     1321 tttacatcag taaataaaaa ttcagctcga atacctggtg taatgctgaa ttttgaagaa
     1381 attttaaaaa tattttctgc aaataaggct agattattac tcttaaattg atagtctgaa
     1441 ttttctaaat ctaaaggatt taagtactta aaatcagcat ctgagttggc tgaacctaag
     1501 ccttgttgtt tcattgtaaa gcctttgaaa taagtagttc caatgcttaa tgaagattgc
     1561 tctttgccta gtttgtattg atacaaaact cttgattcaa taccaaagtt tttatattta
     1621 tctttaatta aatctcgttc tctaaaatcg tcagctcgat tagtataact taaaaatcct
     1681 aatgcatttc ttgaaccaat caagccaaat aaaacggtat tccattttaa attggatttt
     1741 ggactatatt caatatttgc agaaggcata ttccaatcta tgctaaacca atttcttgtt
     1801 cgtgtagatt gttgtgcatc ttgtttatat tgattgtcag tcaatccccc aggttgttgc
     1861 attaaataat tttgatgtgt aaactctaaa gagattttaa gttttgggtt aatttgataa
     1921 cccaattgaa taaatccagt atgcgcattc aaattggaat ttggtcgcca accttttgtt
     1981 cttttatatt gataataggc ataataagtt agattttttg aggtaccttc gatagcatta
     2041 aaaacactaa cagttccaaa agaaccagcc gttgtttgtg aggtaaattg aaatttttta
     2101 tcaattggat gatgtgttct gaaattaacc aaaccaccaa attgagtacc aaattgtaaa
     2161 gaagccgcac ctcgaacgac ttcaattcta tctaaagctt caattggtgg cgtaaaatat
     2221 gcatcaggat agccaatcgc atcggcagtg atgtaatagc cattttgtct tatattaaat
     2281 tcggctgagc gattcggatt taagcctctt gcaccaatgc ctaattgcat tcctgcggca
     2341 tcattttccc aaacattaac gcctgcaata cgtccaaaaa cttgtcttcc attgttggtt
     2401 gctgtattag cttgaagttt agacaaatct ataatttcag tttttttacc ttcataaatt
     2461 attgtaccct caatactttt taaacgaatt tctttatagc gttctgcttc aatagttact
     2521 tcatcaatga ctgttttttg aatactatca taatattgca acaaactgtc tatatcttga
     2581 gcatttagag aatttgcaaa gcataaaagg ataaggaggc aatatatttt aatttttctt
     2641 ttcatcgtat ggtaaaatcc attttttatt taataaattg attggttctt ttgccaaatt
     2701 aattttttca ttgataaaag gacgactagg atgtccgttt aaagcggcat aactttcaac
     2761 ataaactgct gcatcttcaa agcctttatt tttattgtat tcgttggcta aaaaatgagc
     2821 aaactgaata atcatatcag gttgggtact catttgcttt tcctgatgag gtgttagaaa
     2881 atctctattg cgaacagctt caatttttcc gtttttgtct tttattataa attctgttgt
     2941 tgcatttttt tccatcaaca tcacacgcca agaaaaacga taaccttgct ctgtccaaaa
     3001 taaatgtcca ggataaagca aatatcgaaa tggcaataaa atctgaattg aaaaatgaat
     3061 caataataag gtaacccaaa cacggttgaa tttaggttgg taataaatat tttcatttga
     3121 tttataattt ataaaaatgg ataaaaacct ttgatgaaaa tcagctgaga aaaaaatcca
     3181 cgtggcaaca atcatcacat aaggaaaaac accaatttga aataaataac cagtgcaaat
     3241 atggaagaaa acaacagcag cataagcata aggtcgagtt cgcttattta ataaaaagaa
     3301 aaagatagaa atatcaaata acattccgaa ccaagaaaat aaataagcag taatatcgta
     3361 ttcaaataat ttaccaatta taggtagata atcacgactt ttaagccata ttttcaaggg
     3421 ttgagcctcc aacaaccaat caggattaat ctttgcaatt ccagcaaaaa aataaactat
     3481 gccaatttgt aattgaatcg caaggatgca ccaattagga ataaaatttg cttttgatgt
     3541 tatgcctaat tttacatcta atgaagcata tttattagca ggtaaaatgc acatcaataa
     3601 actagcaata ctgataaaat aataatgatt taagtaattg gttttatcta ataattctac
     3661 ataagtaaat gtaagaaaaa ataggagagt agctatacga taaaatgctc ctaataaaat
     3721 acccaaagcc gaaataatca ttattccata aacaatatac atgcctattc cagaaagtgg
     3781 atgcaaccaa agaaaacctt cataaccaaa atgatagctt ggttgtatat ataataaatc
     3841 tacccagcct tttgccacaa agcgaatact gctaaataat atcattgcac caaatagtat
     3901 tctaaaagat gcaagtggag caatggaaac ttgcttaaat aataaatttt ttattgagtt
     3961 tgatgtttct gaattaatca ccatcactat ctgtgtagtt tatagaaaca cctaatgcag
     4021 atggcaaatc cacttttatt aaaactaaca actttttagt ttctaaaaat aatgcatcag
     4081 ctttgggttt attacttgaa acagtaacag agtaatcagc accacaatca ttgaccttag
     4141 cgatacaaac atctatttgg tttaaaatat catcagctaa atttccacca aaagtacttg
     4201 aataacccag agttctaaca taatcaataa tgccttttcc gtctaaagct gctttgccat
     4261 tcaatacatc tttcatgttt tgcaaggtca gtaacatata gtcgatactg tggtcggaat
     4321 agtaagcttg tacagcattt ggtgcagtat atatcgtgct aacatttcct tgataacctg
     4381 caggttttcc tacttgcata cgttttaaat cgtcagttct ttgagcaatt gaattgacca
     4441 tagtactaaa agcacttgat gcatctgtac taacattttc aataaagatt gatgaataat
     4501 tactccaact tgtattgatg gtgctttgga tagtttgtaa attagtaata agcgaaccta
     4561 aatatgtttt gtaagaattt gcatttgcag atgttgtata tctgtctaaa atttcttgat
     4621 tagtgagtgt tctactaaaa agcaaatatt cgatagctgc aagtccttta taatattttc
     4681 ttttactttt tattgaagct actgaattgt ctccacgagt aatataagct tctatactat
     4741 cggcactgca aggataatag tttgaatttt cagtggacat aaccgcatct gctggagcag
     4801 ttttgtcata tatttcacaa tattgccaag cttcgtaggt tgcagaaaat gcattttgca
     4861 aagtattcaa cttcgtaatg tctggagctg ttttaaaagc atcgaaggcg gcttgtaaat
     4921 cacttagttt tgttccataa ttttgatatg caggaataat taagttttct gcataattgg
     4981 taaacatttc ttttttacca aaattatcat cgtcgctact tttcttgtta caagtaacta
     5041 aaattaaaat gattgaaaat aaaaacagtt tattgtattt cattatgatt attgtttgat
     5101 ttttatcaga aaacaatcct gttgaattta cttagattga ttaaagataa tactacaaag
     5161 gtaaaatttg taaatagaaa ttccaattac ctaaatgtta ttttaatacc taaacttggg
     5221 taattttatg ttataaaaca cagatttata caataatata caaaagttta tttcttttca
     5281 ggagttactt gataaccgag ttgttgtaat aattttatta agccagaatc tccagaaaga
     5341 tgtcctgcac ccacagcaat aaaacaagct tccttttcaa tcattggaat tattttatca
     5401 acccattttt tatttctttg gtctaataat gtagttttat aaatacgcat atcatcagaa
     5461 aactccgatt caaattgttc atttaaaaaa ttcaaatctt gctttttata agcacttacc
     5521 attttagtat aattgctttt ttgtgcttcg taatcatcaa taccttttat taaatttttg
     5581 tattgaattt ctaatggaat attttctatg gcttgaagtt gttcttcaat agtttctaaa
     5641 cctgttttcg ttagatggta atcattagca agtagattga gttcttcttc atatactttc
     5701 ttattatcgc caataatact catcataatg aactgatcaa caaaaaatgg ttttaaacga
     5761 ctatacacgt tttcaaacat tgctttgctc attccaaatt gattaattag aaaattttct
     5821 aaatcatcat attctttagg agataaataa tcaagtattg ttttgtcttc tggtaataaa
     5881 cttagatttg caattttcat ttcgtctaat tgcactaaat ccatttccat aaccaaattt
     5941 gtacattcct tgaatttttt aatcgtattt ttgcctataa aataatcatc tattggaatt
     6001 gcgtgtattg ttccaaatag gtaggaattt tgctttaaat tattgccaga tattttccac
     6061 ataagtgcgt tttctatgtt gttttttgga gcagtaggac tatttgcaat aagcatctga
     6121 acgctacaaa aaagcaataa aaagatactt attctttgaa acatattttg tgttttgtta
     6181 ggcaagatag ttgtattttt tataatagat gaagaataaa gtttttttaa gaattcaaca
     6241 ataaatattt ataaaatgca aagccatttg tataaaattt aaaatactaa ttaatttttt
     6301 aatataatga tgtctaaatc ttccaaggta tcaatacttt ttgattgaaa agtagtttct
     6361 tgaacagcga ttttataatt attatctaac caacgtaatt gttctagatt ttcgttgagt
     6421 tcatttttag aaggcgataa ttgaactatt ttatttagaa tatctgaacg aaatgcatat
     6481 aatccaatat gtttatagaa aaaatcttga ttgtttttta tgttttttct tgtaaaagtt
     6541 ttagctttaa tttcattagt taaaattgct tttacaatat taggattgtc taataagctt
     6601 aaatcaaatt ctttttttat ctgtgtgcta atttcgggtt tgtgtagttt caagaaaatc
     6661 gctaattcat caatttgtat aggatttatg aatggctcat cgccttgtat attgatgata
     6721 tattcataag gttcatcgag caaattaaaa gcttcagcac aacgactggt tccgtttaaa
     6781 tgttcactat ttgtaagcaa agctaaaccg ccaatatttt tgatattttg ataaatgtac
     6841 aaactatctg ttgcaacaat gactttgtct aattcatttg ctaaacatgt ttgttcatac
     6901 actcgttgta tcatcgtttt tccattgata ttaaccaaag gttttcctgg aaaacgcaaa
     6961 gaaccaaatc gtgcaggaat aatgcctaaa attttcaaga aaacttagct ttttttactt
     7021 ttatggaata accttcacct ttaatcactt caatggtttc gccttgtaca ataaatgctg
     7081 catcggtaat tgcatcgtaa atattatcat caatctcaac tttgcccgaa ggtcgtaaat
     7141 ctgtaatggc aatacccgtt tttccaacta aatctgaatg gttttcttgt tttatagaaa
     7201 agccttctgt tgatttttga tctgttgtta aaaccatttt gtttaaaatt ggaaaattaa
     7261 aaatagaacc tccaacagta aataataaaa taaatgcaat aagtatagta actgtaaccc
     7321 taaaaatgac catacctatt gcttcaaaaa ttccacctgg caatccaaaa tgttggttgt
     7381 ttactaaact caatactaaa cctgcaataa ttaaaataat tccaagtaca ccagccaccc
     7441 caaagccagg aatgacaaat atttcaagaa gtattaaaat taaaccaatt acaaatagta
     7501 gaatttccca atttgcagcc aagccttcca aataaagtgg agcaaaataa gcgattgcac
     7561 caataatagc aactaaggtt ggcaatccaa tgcccggcat ttttagttca aaataaattc
     7621 caccaataat catcatgatt aaaagagaat tgataaatgg atttaataaa aaggagatta
     7681 ttttatcaat cgtagtttca acgtgtgaga tgaccacata aggttctggg ctgatatgtt
     7741 taaaaacact ttctttagag tttaaaatag catcacaata atgatttttt agagcttctt
     7801 gtgttgtgaa agtgagaatt tttccagtat cttttatacc cggaacttga acatcttcat
     7861 cgaccatagc ttcagcaatt tttggatttc taccatttgt ttctgctgta cttcgcatta
     7921 atgaacgcat ataagattgg tatttatctg gtgctttttc gccactttga ttcacgaccg
     7981 ttgctgctcc gatattggca ccttcaatca taaaaatact atcacatgca atggatatta
     8041 aggctccagc cgacgctgca ttgttattta tccaaacaac tgtttttaat tttgtattca
     8101 ataaagtact tctaatttca tctgcggcat ctaataaccc gccaaaagta ttcaaatcta
     8161 agacaaaaaa agtagctttc tgtttttcag cttcttcaat tcctttgtga atcaatcttg
     8221 ctgcagcttt atcaatttct gcatgtaatt ttaaataata aattttatta tcctttgcat
     8281 aagaaatgct acatagcaat aggagtgtgg ctaatatctt gtttttcata gacttcactt
     8341 ttgttgataa attcaatgca attataagga aaataggcat aaaatttgca tataaaatgt
     8401 caatatgata aattataagt tgaaggtgca ttttctcgtt ggagtatttt tgtttttaag
     8461 ttttggattc gcaattgcac aaaatttcat aattcataaa gtggaaaaag gagagacttt
     8521 atacggactt tcaaagaaat ataaaactac tgttgatgct atttataaaa ataatcctgc
     8581 tttaacctct gataatttaa aattgggtac agaacttagg attcctcaac ctaaagaatt
     8641 gattaaaaag cctactacgc cgacaactac acctttacct tcagtatctg ataataagaa
     8701 agtagaaatt aaatctacta tgattgctaa agctaagata gacacaattt tacatcaaat
     8761 tcaagctaaa gaaactttat atggtctatc caaaaaatac aatgttagta ttgatgatat
     8821 tagaaaatgg aataaactct cagatgcagg tattcaaata ggaatgattt tgaaaatatt
     8881 aaaaccggct tcaatttcta ttaatccaaa tgaaaaagtt gaagtggaga aaaaaaatga
     8941 agcaatacaa ccaactgttg tagagaaagt tgttgtgctg cctgaaaaac aaacacctat
     9001 aaaaattgat accgaaaaag tagaaccacc gaaagaagtt gaaaagccag tagctcctaa
     9061 aactttatcg ctatacgaat tgtttgccaa tcaacccaaa agtgctttaa agaaaattaa
     9121 agcaactgga gctccaatga atacaactga tgaaagtatt gataatacat tttttgcttt
     9181 acataaaaca gctcaattgg gtagtgttat taaagtaaaa aatcttgaaa acaataaagt
     9241 aagttatgca aaagtaatcg gtaaattacc tgatactgat gaaaacagaa aagtattagt
     9301 tcgtctgtcg cttggtgttc gtaatgcgat taaaatgaac aatggcaaag cctatttaga
     9361 aatggaattt atcaatgaat aagattagat aaatcataat aataaactaa tagagtttaa
     9421 aaatccattt cttacctttg ttgtttagat agatatgggg aaaataggtg ttttattagt
     9481 taatcttggt actccagatt caccatcaaa tagtgatgtc tataaatatc tgattgaatt
     9541 tctgacagat aaacgtgtga tggatttcgg ttggttgaaa agaaatctat tagttcgtgg
     9601 aattattgtt ccagctagat ataagcattc tgcaaaaaca tatagagcta tttgggataa
     9661 agatagaggt tcgcctttgt tgtttcatac agaagattta ggcaagaaag tgcaatcaat
     9721 attaggcgat aaatacgtcg ttgaaattgc aatgcgttat caaaatccga gtattgaaag
     9781 tgctttgaag aaactgcaag ctcaattagt ttctgaaatt atagttttgc cattgtttcc
     9841 tcaatattca tcggcttgca atggttctat aatacaaaaa gtacaagaaa taacaaacaa
     9901 gtggctcaca ttaccaaagc taacgtatat taatcatttt tatgacaata aattgtttat
     9961 tgatgccatt gttgaaaatg caagacaatt taatttgaat gattatgacc atattatttt
    10021 tagttaccat ggtttgccgt taagacattt actaaatgga gatattactg gtttgtattg
    10081 taaagcagat aaaacatgtt gtaatacaat aactgaggtt aatcaaaatt gttatagaac
    10141 ccaatgtttt caaactacaa aattagttgc tgaacaatta ggtctttctt cggataaata
    10201 taccaataca tttcaatcaa gattaggcaa agaaatttgg atagaaccgt attctgttga
    10261 tgttattcca gcattagcca aacaaggaaa acgaaagtta ttagtttttt cgccagcatt
    10321 tgttgccgac tgtttagaaa cattatttga aatttctgta gaatatcaag aattatttga
    10381 agaacacgga ggacattctg ttcaattagt tcctagtctg aatagcaatc aaacttgggt
    10441 aaatgctgtt gctgatattg tgatgaagaa tcaattaaag aaataataga agccgagaat
    10501 tataaaggca attctagtaa attatcattg tgtagtttga tgaaattcat gcctacatta
    10561 tattcttttg aaaaaacttc ttttagtttt tcataagcaa aaacattact tatccaatct
    10621 acaccattga tgtccacaat gacaaattta ttttcaggat tttgtctaaa atattcaaaa
    10681 tacatacgtt caattttgtt caaatatact ttagaaatag attgctcgta atcacgacct
    10741 cttcttttga tatttcgcaa aagggtttct gtatcggaat gtagatagaa aataatatca
    10801 ggtttgggta gttttttata gattaaatta aaaaaagaat gatagagttt aaactcttgt
    10861 ttagacaaat taattttagc aaaaagcaaa cattttttaa gcatataatc agtaatgata
    10921 aattgcgaaa agatatttgg ctcagcaaaa attttagtca attgttggta gcgttcaatc
    10981 aaaaatgaca tttctaattg taatccatat ttctcaggct gttggtagaa tttactcaaa
    11041 taaggattgt cagaaaaggc ttccaaaatt aattgaccac catattcttc tgaaagtaaa
    11101 cttgctaaag tagtttttcc tgcacctata tttccttcta ttgtgataaa tttatacatt
    11161 cagggaaatt tactacttaa attgaaatgg t
//
LOCUS       DAOQCW010000083        11025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_2221_length_11025_cov_6.345761, whole genome shotgun sequence.
ACCESSION   DAOQCW010000083 DAOQCW010000000
VERSION     DAOQCW010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11025
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2221_length_11025_cov_6.345761"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3402
                     /gene="mfd"
                     /locus_tag="PLS16_08170"
     CDS             <1..3402
                     /gene="mfd"
                     /locus_tag="PLS16_08170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNJ61095.1"
                     /translation="FPYLCIRMSLQHLLELFRDDERTNSILQNLSTQKQILLQHCIGS
                     APAFVLTALFQKTDYTHLFIADDSEQAMYILNDLQNILPKTQILYLPSSYRKQNSFEE
                     LSNNNILTRAQTLNALLRQKKDGNIIVTYPEALLEKIVHAQKLQENTLALKVGEKVDV
                     DFILDVLIEYGFERTDFVYEPGEFSIRGGIIDIFSFGNEQPYRIELFDDEVESMRTFE
                     VETQISIQKISELTIVPNVQTHFTDEQHADIFEYLPEKTLIWFNDTSIFVELIEKYFE
                     RALGEFYAIQENSKIKEHHFKQKTLQQLFVLPETLFGHIEQKNILAFKTNQNYFLKSD
                     TQKWFTIDYLQVPQPSFNRNFDLLIKDLQLNQKEQFSNYIFSENAKQIERFEHIFKDK
                     NADIKFIPVYIPLAQGFVDRNLGIACYTDHQIFERYFKYKTKQGYTRNKAISIKQLKD
                     LNPGDYVVHIDHGIGKFSGLQIMNTNGVEQEMVRIIYKDNDILYVNINSLYKISKYTG
                     KEGTIPKVHKLGTNIWNTLKQKTKAKVKDIAQELIALYAKRKVEVGFAFQADTYLQDE
                     LEASFMYEDTPDQIKATLDIKQDMEKPHPMDRLICGDVGFGKTEVAIRAAFKAVNDSK
                     QVAVLVPTTILAWQHFKTFSKRLEDLPVKVDFINRFKTAKEKTATLKELEEGKIDILI
                     GTHALLNKKIKFKDLGLLVIDEEQKFGVAAKEKLRQMATNVDTLTLTATPIPRTLKFS
                     MMGARDLSNIMTPPPNRIPVTTEVHTFNVEFLKEAIEYEVYRGGQVFFVHNRVKDLHE
                     IDILLKRLMPDIKINAAHGQMEGDKLEEIMLEFINGEFDVLLSTNIVESGLDIPNANT
                     IIINDAQNFGLSDLHQLRGRVGRGNKKAFCYLLAPPKSSLTSEAKKRLQTLEEFSDLG
                     SGFQIALRDMDIRGAGNLLGGEQSGFITDIGFEMYHKILDEAITELKHTEFKEVFKEQ
                     IEAQTEFVRDCSIDTDLEMLIPNNYIQNTEERLRIYTQLDEISNEAELTTFTQNLNDR
                     FGKIPKEVNELFDGLRIRWLAKKIGFERIILKGNKLRCYFLDNPNSPYYESGNFGKVM
                     QYIQEGKKRCILKQNGNNLILTYENIHTMNSAEKILQDIVSAVLI"
     gene            complement(3406..4272)
                     /locus_tag="PLS16_08175"
     CDS             complement(3406..4272)
                     /locus_tag="PLS16_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61096.1"
                     /translation="MNILSELKKERAFKDLFADYFKLIKSTFFHFSINFILLALPLSW
                     FISFLLIDKGPIVLKKIILFYFNRHFNEYLIYTILSLPLIIAAYCIQLALCILFFSIY
                     SVEYMLLLELRKSKNFNIMHVLVNSIKHLPNYFSFLLLVILGFPILVLIFGILSCIPF
                     SFIFIIPFLLLFFNSTVINSITNKKGLFQSFFSILKFLKTQSLSNLFAGFLILVIFFT
                     FEILFFNFFPNLFSSLGLSYEPSSVHKNIEKLSYLRIIPLGIVVIYIANFINQFFILQ
                     YFSIDTNTEDRV"
     gene            complement(4362..5615)
                     /locus_tag="PLS16_08180"
     CDS             complement(4362..5615)
                     /locus_tag="PLS16_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61097.1"
                     /translation="MILLFNVVSLENIKEFWWLYLSMPFIAAIIGYGTKIVAIKMMFE
                     PIEFFGIKPFLGWQGIIPKKAAIMSAIACDTLTSRLIKPEEIFARLDPERVAQELEKP
                     MLPIVEKITHEVMTHYQPGLWESIPENAKRLLIKQIQRESPDMVRQLMQETKDNLNNV
                     FDLKDMVITNLTRNKKLLNKIFYESGEKEFQFIKHSGIYFGFMIGLVQMIVWMLTQEP
                     LVMPIFGLFTGWFTDWLALKMIFAPQQEKKFLGIFKWQGLFFKRQQEVASAYGALIAK
                     EILTPSNMIESILTGKLSDNLFNMIQKNIQQMVDSQSGILKPIVVFAVGSDKYKAMKN
                     SITENMIKQLPVALKHIEKYAEDAMDIQNTLVTKMQELTPDEFEGVLRPAFKQDEWIL
                     ITVGAVLGFMVGELQVLLMEFFVLH"
     gene            complement(5669..6061)
                     /locus_tag="PLS16_08185"
     CDS             complement(5669..6061)
                     /locus_tag="PLS16_08185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNJ61098.1"
                     /translation="MTIFSKIISGEIPSYKIAEDENYYAFLDIRPLNAGHTLVIPKVE
                     IDYIFDLDDDMLSGLILFAKKVAKGIERVVPCERIGVAVIGLEVPHCHIHLSPINSVH
                     DLDFRNTKSISKEEMQSLATKISQEIDL"
     gene            6186..7466
                     /locus_tag="PLS16_08190"
     CDS             6186..7466
                     /locus_tag="PLS16_08190"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HNJ61099.1"
                     /translation="MKEALLHFIWRYQLVHPDRLETTNGDIIEIIHPGTYNKNESADF
                     LDAKIKIGELIFVGNIEIHINSSDWRKHQHQHNKKYNNVILHIVYNDDEPLENVPTLE
                     LNGKIPPYILQQYGQLMQSKLPVPCQFDWKNLTRLEQVAFQDRFILERLNNKSKKILM
                     TLQYCNNDWLQTKYALLLKYFGGNVNQFGFTELATRLDYKILIKHADNLFQIEALLFG
                     TAGFLEDDIQDNYYENLKKEFQYLQRKYNIVSLKKTIWNFMRMRPASFPTIKIALLAA
                     LIHASPQLYQMQHIENAQVFLANITASNYWDRHYQFGKESNLLVKHIGKSSIHALLIN
                     YNIPLFYTYQTTFEQKDVLQTCIEYYETLPFEQNHKIKYFKTNISKPSALDSQVLIEL
                     YDAYCSPKRCLDCPIGFKILKNKHFQRQELIRNA"
     gene            7488..7742
                     /locus_tag="PLS16_08195"
     CDS             7488..7742
                     /locus_tag="PLS16_08195"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNJ61100.1"
                     /translation="MNFIEKPIKVFQSHSMRDAIEMSVYGVCSYLGRKMSIPSKKVRL
                     FFIYTSFIALGSPIIIYLILTFVLNLKHMVKTKRNPVWDI"
     gene            complement(7788..8306)
                     /locus_tag="PLS16_08200"
     CDS             complement(7788..8306)
                     /locus_tag="PLS16_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61101.1"
                     /translation="MKKVILFFSAIVLFSLTIMAQPPQKPMYNKGGGGAYNKENIQAM
                     KIAFLTKHLALTELQATSFWPIYNKYEAEKKVLRKNTIGEEKLSKSLDEISDIDANKV
                     IDNYYSLKTKELELSKKYTEDFKKVLEPKQVAKLITSEAQFKKMLMQGHNGYNKQGPP
                     KPQIQPTKPVTE"
     gene            complement(8323..8841)
                     /locus_tag="PLS16_08205"
     CDS             complement(8323..8841)
                     /locus_tag="PLS16_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61102.1"
                     /translation="MENNKEILDELNAVAPLLNSLKKESSEVVIPDNYFDNFQTSVCS
                     QVKEERTNVVSIKRNPFRIIKIGIAASVFLAIFFLVVHFKNANNQAEIIVKTPASSTE
                     IQHYITENINDFTVEEVGQFVAMQTNNKIQTEAITTSSNAVMTDEEINQFVENNPVYI
                     SDLYDDETATIF"
     gene            complement(8878..9402)
                     /locus_tag="PLS16_08210"
     CDS             complement(8878..9402)
                     /locus_tag="PLS16_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNJ61103.1"
                     /translation="MENLVEQLQQLETKESAFKTLMDLYQRKLYFHIFRFVNNHEDTN
                     DVLQNTFIKVWRNIGNFRNECSLYSWLYRIAGNEAITFINSNKKRITVDVDTKNVANI
                     GNVDSIEGDKIELKLQAALEQLPEKQKQIFILRYYDEMPYKEMSEILETSEGALKASY
                     YHAAKKIETFLLEH"
     gene            9491..10054
                     /locus_tag="PLS16_08215"
     CDS             9491..10054
                     /locus_tag="PLS16_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNJ61104.1"
                     /translation="MAATPTTHIPLGYTAPDFTLLDTISGNLKSLQELKGQKATVVMF
                     ICNHCPYVKHVNQQLVQLANDYIPKDIAFIAISSNDIENYPDDAPEKMTSIAKEEKYP
                     FPYLYDETQAIAKAYHAACTPDFSIFDKDLKCVYRGQLDDSRPKNDSISDGKDIRNVL
                     DCILNDTPIPEKQIPSLGCNIKWKPEV"
     gene            10058..10774
                     /gene="recO"
                     /locus_tag="PLS16_08220"
     CDS             10058..10774
                     /gene="recO"
                     /locus_tag="PLS16_08220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNJ61105.1"
                     /translation="MLEKTEVIVLKATKYGESSLIVKLFSEKFGCIPVILKGIRNGKK
                     NNAGLFQPGYILELNIYYRENKQLFQVKDYAVAYNYQSIAQNIGKQSIVLFIVELLLN
                     VLREHHPNKEIFFYIKDFILKLDNEEHKIKEYPLTLLYELSQFLGFEPFIGNQFINSY
                     FNLEKGSFEQDYIPQLCLDTRTSKLFFDYIHCLNEQIIHEITANEQQALLEIWLNYYR
                     FHIPEFRTMQTPKILHEVLH"
     gene            complement(10771..>11025)
                     /locus_tag="PLS16_08225"
     CDS             complement(10771..>11025)
                     /locus_tag="PLS16_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNJ61106.1"
                     /translation="EPIWAIGTGKTATPEQAQEVHHFIRKCIAENYNATIADQISILY
                     GGSVNAQNAAQLFSQADIDGGLVGGASLKAQDFRTIILSI"
BASE COUNT         4006 a         1672 c         1577 g         3770 t
ORIGIN      
        1 tttccgtatc tttgcattcg catgagtttg caacatctct tagagttgtt ccgtgatgat
       61 gaacgaacca actctatttt acaaaatcta agtactcaaa aacaaatttt gttgcagcat
      121 tgtattggct ccgctcccgc ttttgtattg actgcattat ttcaaaaaac agattataca
      181 catcttttca ttgctgacga ttcagagcaa gctatgtata ttttgaatga tttgcaaaat
      241 atacttccaa aaacacaaat tctttattta ccttcttctt atcgtaaaca aaatagtttt
      301 gaagagttaa gcaataataa tattctgact cgagcacaaa cgctcaatgc actattaaga
      361 cagaaaaaag acggcaatat tattgttaca taccctgaag cattactcga aaaaattgta
      421 catgcccaaa agttacaaga aaatacttta gctctaaaag ttggcgaaaa agttgatgtt
      481 gattttattt tagatgtact tattgaatat ggctttgaac gtaccgattt tgtttatgaa
      541 cctggtgaat tttccattag aggtggaatt atagatattt tttcatttgg aaacgagcaa
      601 ccatacagaa ttgaattatt tgatgatgaa gtagaaagta tgcgaacatt tgaagtagaa
      661 actcaaattt ctattcaaaa aatttcagaa ttgacgatag ttcctaatgt tcaaacgcat
      721 tttacagatg agcaacatgc cgatattttt gaatatctac cagaaaaaac cttaatttgg
      781 ttcaatgata caagcatttt tgtagaatta attgaaaagt attttgaacg tgcattgggc
      841 gaattttatg ccatacagga aaattctaaa attaaagaac atcattttaa acagaagaca
      901 ctacaacaat tattcgttct gcctgaaaca ctttttggac atattgaaca aaaaaatatc
      961 ttagctttta aaactaatca aaattatttt ttaaaatctg atactcaaaa atggtttacc
     1021 atagattatt tacaagtacc acagccttct tttaatagaa attttgattt attaataaaa
     1081 gatttacaac tgaatcaaaa agaacaattc agcaattata tattctctga aaatgccaaa
     1141 caaattgaac gttttgagca tatttttaaa gataaaaatg cagatataaa attcattcct
     1201 gtatatattc cattagcaca aggatttgta gatagaaact taggaattgc ttgttataca
     1261 gaccatcaaa tatttgagcg ttattttaaa tacaaaacca aacaaggcta tactagaaac
     1321 aaagccataa gcataaagca attaaaagac ttaaatcctg gagattatgt ggttcatatc
     1381 gaccatggaa ttggtaagtt ttcaggtttg caaattatga ataccaatgg cgttgaacaa
     1441 gaaatggtac gaattatcta taaagataat gacatcttgt acgttaatat caattcatta
     1501 tacaaaattt caaaatacac tggcaaagaa ggcactatac caaaagtaca taaattaggt
     1561 acaaatattt ggaatacact caaacaaaaa actaaggcga aagtaaaaga tattgctcaa
     1621 gaactaattg ctttatatgc caaaagaaaa gtagaagtag gctttgcttt tcaggctgat
     1681 acctacttgc aagatgaatt agaagctagt tttatgtatg aagatacacc tgaccaaatt
     1741 aaagcaacat tagacatcaa acaggatatg gaaaaaccac atcctatgga tagattgatt
     1801 tgtggtgatg ttggatttgg gaaaacagaa gtcgcaatac gtgcggcttt taaagcggta
     1861 aacgatagca aacaagttgc tgttttagtg ccaacaacta ttttagcttg gcaacatttc
     1921 aaaacatttt ctaagcgttt agaagatttg ccggtaaaag tagattttat caatcgtttt
     1981 aagactgcta aggaaaaaac tgcgacttta aaagaattag aagaaggaaa aatagatatc
     2041 ttaattggta cacatgcact tcttaataag aaaataaaat ttaaagattt aggcttattg
     2101 gttattgatg aagagcaaaa atttggcgta gctgcaaaag aaaaattaag acaaatggca
     2161 acaaatgtcg ataccttaac attgacagcc acaccaattc ctcgtacatt aaaattctct
     2221 atgatgggtg cgagagattt gtctaatatt atgacaccgc cacccaatag aattcctgta
     2281 actactgaag tacatacttt taatgtagaa tttctaaaag aagcaattga atatgaagtt
     2341 tatcgaggtg gtcaagtatt ctttgtacac aatcgagtaa aagatttgca tgaaattgat
     2401 atcttattga aacgattgat gcctgatata aaaattaatg cagctcacgg acaaatggaa
     2461 ggcgacaaat tagaggaaat tatgttggaa tttatcaatg gagaatttga tgttttgctt
     2521 tctactaata ttgttgaatc gggtttagat atcccaaatg ccaatacaat catcatcaat
     2581 gatgctcaaa attttgggtt aagtgattta caccaattgc gtggtcgtgt gggtcgtggc
     2641 aacaaaaaag ccttttgtta tttattagca ccgccaaaaa gttcattaac atcagaagcg
     2701 aaaaaacgtt tacaaacatt agaagaattt tcagatttag gttcaggttt tcaaattgct
     2761 cttcgagata tggatattcg tggggcagga aatttattag gtggcgaaca aagtggtttc
     2821 attacagata ttggatttga aatgtatcat aaaatattag acgaagccat tacagaatta
     2881 aaacatacag aatttaaaga agtatttaag gaacaaattg aagcacaaac tgaatttgta
     2941 agagattgca gtattgatac cgatttggag atgttgattc caaataatta catacaaaat
     3001 actgaagaaa gattaagaat ttacacacaa cttgatgaaa taagcaatga agctgagctt
     3061 actactttta cacaaaactt aaatgataga tttgggaaaa tacctaagga agtcaatgaa
     3121 ctatttgatg ggttgcgaat aagatggtta gctaaaaaga taggttttga acgtattatt
     3181 cttaaaggca ataaactacg atgttatttt ttagacaatc caaattctcc ttattacgaa
     3241 agcggtaatt ttggaaaagt aatgcaatat attcaagaag gcaaaaaacg ttgcatactc
     3301 aaacaaaatg gcaataattt aattcttact tacgagaata tccatacaat gaatagtgct
     3361 gaaaaaatat tgcaagatat cgtaagtgca gtgcttatct aaatttcata ctctgtcttc
     3421 tgtatttgta tcaatgctaa agtattgtaa gataaaaaat tgattaataa aatttgcaat
     3481 ataaatcact acaataccca aaggaataat tcttaaatag cttaattttt cgatgttttt
     3541 atgaacactt gatggttcat aagataatcc aagagaacta aatagattag gaaaaaaatt
     3601 aaagaaaagt atttcaaaag taaaaaaaat cacaaggata agaaatccag caaagagatt
     3661 actcaaactt tgggttttta aaaattttaa aatagaaaag aaagattgga acaatccttt
     3721 tttattagta atagaattaa taactgtgct attaaaaaac aatagtaaaa aaggaataat
     3781 aaatataaac gaaaaaggaa tacaactcag tattccaaaa attaaaacaa gtatcggaaa
     3841 cccaagaatt accaataata agaaggaaaa ataattcggc aaatgtttta tactatttac
     3901 taaaacatgc attatattga aatttttact ttttcgcaat tctagcaata acatatactc
     3961 tacgctataa attgaaaaaa ataagatgca taaagcaagt tgaatacaat aagcagcaat
     4021 aataagtgga agtgataaaa tcgtataaat taggtattca ttaaaatgtc tattaaaata
     4081 aaataatatt atttttttta gcacaattgg tcctttatca atcaataaaa aagagataaa
     4141 ccaagaaagc ggcaatgcta ataaaataaa atttatacta aaatgaaaaa aggtactttt
     4201 aattagttta aagtaatccg caaataaatc tttaaaagct ctttcttttt ttaattcaga
     4261 taatatgttc atcgcttcgc agttctttgt tgaaatctaa tcaagtattt taatcagacc
     4321 taaaattaaa aataaagcgt ttttatcaat aaaaacaaat tctaatgcaa tacaaaaaat
     4381 tccattagta gcacttgtaa ttccccaacc ataaagccca aaacagcacc tactgtaatt
     4441 aatatccact catcttgttt gaaagctggt cttaatacgc cttcaaattc atctggtgtc
     4501 aattcttgca tttttgtcac caaagtattt tgaatatcca tcgcatcttc agcatatttc
     4561 tcaatatgtt ttaaggcaac tggaagttgt ttaatcatat tttcagtaat agagtttttc
     4621 attgccttat atttatctga acccaccgca aaaactacga ttggttttaa aattcctgat
     4681 tggctatcaa ccatttgctg tatattcttc tgaatcatat taaacaaatt atctgatagt
     4741 tttccggtca aaatggattc tatcatattg cttggtgtta gaatttcttt agcaattaaa
     4801 gcaccatagg cagaagccac ttcttgttgt cttttgaaaa acaaaccttg ccatttaaaa
     4861 atccctaaaa actttttttc ttgctgtggt gcaaaaatca tttttagtgc caaccaatct
     4921 gtaaaccaac ctgtaaataa tccaaaaata ggcataacca atggttcttg cgtaagcatc
     4981 caaactatca tttgtaccaa acctatcata aaaccgaaat aaattccaga atgcttgata
     5041 aattgaaatt ctttctctcc actctcataa aatatcttat tcaacaattt tttatttctt
     5101 gttaaattag tgataaccat atctttcaaa tcaaaaacat tattcaagtt atctttggtt
     5161 tcttgcatca attgccgcac catatctgga gattctcttt gaatttgttt gatgagcaaa
     5221 cgttttgcat tttcaggaat actttcccac aaaccaggtt ggtaatgtgt cattacttca
     5281 tgtgtaattt tttccacgat aggcagcatc ggtttttcca actcttgagc aacacgttct
     5341 gggtctaatc ttgcaaaaat ctcttctggt tttatcaatc ttgaagttaa agtatcacaa
     5401 gctatcgcac tcattattgc agctttttta ggtattatgc cttgccaacc caaaaacggt
     5461 ttgataccaa aaaactctat tggttcgaac atcattttta tagctacaat cttagttccg
     5521 taaccaatta ttgcggcaat aaatggcatt gacagataga gccaccaaaa ctcttttata
     5581 ttttctaaac ttactacatt aaacaataaa atcatataca attaatactt ttgtgaatat
     5641 cggattttta gagcaaaaaa aggcgtaatt acaaatctat ttcttgacta atttttgtag
     5701 ctaaactttg catttcctct ttggaaatac ttttggtatt tctaaagtct aaatcgtgta
     5761 cagaattaat aggcgataaa tgaatatggc aatgtggcac ttccaatcca atgactgcaa
     5821 cgccaattcg ttcacaaggc actactcgtt caatgccttt ggctactttt ttagcaaata
     5881 aaattaagcc tgaaagcata tcatcgtcta aatcaaaaat ataatcaatt tccacttttg
     5941 gaataaccaa agtatgtccc gcatttaatg gtctgatatc taaaaatgca taatagtttt
     6001 catcttcagc aattttataa gaaggtattt cgcctgaaat gattttagaa aatattgtca
     6061 tcgtaaatat aatttaggtt taaaaataag tatttacaag tattttttaa ttgattttat
     6121 ggaaaataga atataattcg tcatttaatg gagtaaaata ttgtttatgt tagctaaata
     6181 cgattatgaa agaagcctta ttgcatttta tttggagata tcaattagta catccagata
     6241 gattagaaac taccaatgga gatattattg aaattattca tccaggcact tataataaaa
     6301 atgaaagtgc cgatttctta gatgcaaaaa tcaaaattgg agaattaatt ttcgttggaa
     6361 atatagaaat tcatatcaat tcctctgatt ggcgaaagca tcaacatcag cataataaaa
     6421 aatacaacaa tgtaatctta catattgtat ataatgatga tgagccatta gaaaacgttc
     6481 ctactttaga actcaacgga aaaatacctc catatatttt acaacaatat gggcaactga
     6541 tgcaatcaaa acttccagtt ccttgtcagt ttgattggaa aaatttaacc cgtttagagc
     6601 aagttgcatt tcaggataga tttatcttag aacgattaaa taacaaatcg aagaagatat
     6661 taatgacctt gcaatattgt aacaatgatt ggttacaaac caaatatgct ttattattga
     6721 aatattttgg cggaaatgtc aatcaatttg gctttacgga attagctact cgattagact
     6781 ataaaatact aataaaacat gctgataact tattccagat tgaagcattg ctattcggaa
     6841 cagctggttt tttggaagat gatattcaag ataattatta cgaaaatcta aaaaaagaat
     6901 ttcaatattt acaacgtaag tacaatattg ttagtttgaa gaaaacaatt tggaatttta
     6961 tgcgaatgcg acctgctagt tttcctacta taaaaatcgc tttgttggct gcattaattc
     7021 atgcatctcc acaattatat caaatgcagc atatcgaaaa tgcacaagta ttcttagcaa
     7081 atattaccgc atctaattat tgggataggc attaccaatt tggtaaagaa agtaatttat
     7141 tagtaaaaca tattggcaaa tccagcatac acgcacttct tatcaattac aatattcctt
     7201 tattttatac ctatcaaaca acatttgaac aaaaagatgt tttgcaaact tgtattgaat
     7261 attatgaaac attacctttt gaacaaaatc acaaaatcaa atattttaaa accaacattt
     7321 ctaaaccgag tgctttagac agtcaagtat tgattgaatt atatgatgca tattgctctc
     7381 caaaaagatg tttggattgt ccaattggat tcaaaatatt aaaaaataaa cattttcaga
     7441 ggcaagaatt aataagaaat gcctaacttg catacaaatt atagacattg aattttatag
     7501 agaaaccaat taaagtattt caatcacata gtatgcgtga tgctattgaa atgagtgttt
     7561 atggcgtttg ctcgtattta ggcagaaaga tgtctatccc atctaaaaaa gttcgtttgt
     7621 tttttattta taccagcttt attgccttag gctctcctat tattatttat ctaattttaa
     7681 cttttgtttt gaatttaaaa cacatggtta aaactaaaag aaatccagtt tgggacatat
     7741 aaaaaatagc caccaagtgg cggctatttt atttagtaat tttgaaatta ttctgttaca
     7801 ggctttgttg gttgaatttg aggttttgga ggtccttgtt tgttatatcc attatgacct
     7861 tgcatcaaca ttttcttaaa ttgagcttct gaagtaatta acttcgctac ttgtttcggt
     7921 tctaatactt tcttaaaatc ttcagtatat tttttggaaa gttccaattc ttttgttttt
     7981 aaagaataat aattatcaat tactttattc gcatcaatat ctgaaatttc atctaaactc
     8041 ttacttaatt tttcttctcc aattgtattt tttcgaagta ctttcttttc ggcttcatac
     8101 ttattataga ttggccaaaa acttgtagct tgtaattctg tcaatgctaa atgcttagta
     8161 agaaaagcaa ttttcattgc ttgaatattt tctttattat aagctccgcc tccacctttg
     8221 ttatacattg gtttttgtgg tggctgtgcc ataattgtta aactaaacaa tactattgca
     8281 ctaaaaaata aaattacttt tttcatgatt aaaaatttat aattaaaaaa tagttgctgt
     8341 ttcatcatca tataaatcac ttatataaac tgggttattt tctacaaatt gattaatttc
     8401 ttcatctgtc attactgcat ttgatgacgt tgtaatcgct tcagtctgta ttttattatt
     8461 tgtttgcata gcgacaaact gtcccacttc ctcgacagtg aaatcgttaa tattttcagt
     8521 aatataatgt tgtatttctg tcgaagatgc aggcgttttt actataattt ctgcctgatt
     8581 attagcattc ttgaaatgaa ccactagaaa aaatattgct aaaaatactg aagctgcaat
     8641 tccaattttt ataattctaa atggatttct tttaattgaa acgacattag tacgctcttc
     8701 tttaacttgt gagcaaacac ttgtctgaaa attatcaaaa taattatcag gaataactac
     8761 ctctgaactt tcttttttca aagaatttaa aagtggtgct actgcattta attcatctaa
     8821 tatttcttta ttattttcca ttgttctata atttagactg attaaataat taaaggttta
     8881 atgttctaat aaaaaagttt caattttttt tgctgcatgg taataagaag ctttcaaagc
     8941 gccttctgat gtttctaaaa tctcactcat ttccttatac ggcatttcat cataatatct
     9001 caaaataaaa atctgctttt gtttttcggg taattgttct aaggcagctt gtaatttcaa
     9061 ttcaatttta tcaccttcta ttgaatcaac atttccaata tttgcaacat tctttgtatc
     9121 aacatctact gtaattcttt ttttattact attgataaat gtaatcgctt catttccagc
     9181 aattcgatac aaccaagaat acaacgaaca ttcatttcta aaatttccaa tatttctcca
     9241 aactttaata aaggtatttt gcaaaacatc attcgtatct tcatgattat taacaaaacg
     9301 aaaaatatgg aaatacaatt tcctttgata taaatccatc aaagttttaa aggcactttc
     9361 ttttgtttcc aattgttgta attgttctac caaattctcc attcgctaag acttacaagt
     9421 ttagactaaa aattttgcca aaagtttaat taaggctatt tttgtttttt aatcatcaaa
     9481 gataattatt atggcagcaa caccaaccac acatatccca ttgggttata ccgcaccaga
     9541 ttttacatta ttagatacaa taagtggcaa tttaaaatcg ctacaagaat taaaaggtca
     9601 aaaggctacg gttgtgatgt ttatttgcaa ccattgtcct tatgtcaagc acgttaatca
     9661 acaattggta caattagcca atgattatat tcccaaagac attgcattta ttgctatttc
     9721 aagtaatgat attgaaaatt atcctgacga tgctcctgaa aagatgacga gtattgcgaa
     9781 ggaagaaaaa tatccatttc cttacttata tgacgaaaca caagcaattg caaaagccta
     9841 tcatgcggct tgcacaccag atttttcaat ttttgataaa gatttaaaat gtgtatatcg
     9901 agggcaatta gatgattcta gaccaaaaaa tgatagtatt tctgatggaa aagatattag
     9961 aaatgtatta gattgcatat taaatgatac accaattcct gaaaagcaaa tcccaagttt
    10021 aggttgtaat attaaatgga aaccagaagt ttaatttgtg ctagaaaaaa ctgaagttat
    10081 tgttctaaaa gccacaaaat atggcgaaag tagtttgatt gttaaactat tttctgaaaa
    10141 atttggttgt attcctgtaa ttttaaaagg catcagaaat ggcaaaaaaa ataatgctgg
    10201 tttatttcaa cctggatata ttttggaatt gaatatttac tacagagaaa ataaacaact
    10261 atttcaggtt aaagattatg cagttgctta taactatcaa agcattgctc aaaacatcgg
    10321 taaacaaagt attgttttat ttattgttga attattattg aatgttttac gtgaacatca
    10381 tcctaataaa gaaatctttt tttatatcaa agattttatt ttaaaattag ataatgaaga
    10441 acataaaata aaagaatatc ctttaactct cctatatgaa ctttcacaat ttttaggctt
    10501 tgagcctttc attggaaatc aatttataaa ttcctatttt aatttagaaa aagggagttt
    10561 tgaacaagat tatattcctc aactttgttt agatactcga acgtctaaac ttttttttga
    10621 ttatattcat tgtctaaatg agcaaattat tcatgaaata actgccaatg aacaacaagc
    10681 tttattagaa atttggttga attattatcg tttccatatt cctgaattta gaactatgca
    10741 aactccaaaa atcttacacg aggtattaca ttaaatagat aaaataatcg tcctaaaatc
    10801 ttgagcttta agagatgctc caccaaccaa accaccatca atatctgctt gagagaataa
    10861 ttgtgctgca ttttgtgcat ttacactgcc accatataat atactaatct ggtctgctat
    10921 tgttgcattg taattttcgg caatacattt tcttataaaa tgatgtactt cttgtgcttg
    10981 ttctggtgtg gctgtttttc ctgttccaat tgcccaaatt ggttc
//
LOCUS       DAOQCW010000084        10915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_2246_length_10915_cov_5.683886, whole genome shotgun sequence.
ACCESSION   DAOQCW010000084 DAOQCW010000000
VERSION     DAOQCW010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10915
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2246_length_10915_cov_5.683886"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            525..659
                     /locus_tag="PLS16_08230"
     CDS             525..659
                     /locus_tag="PLS16_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61107.1"
                     /translation="MKKPKTRYELPPTIVGTTVATTSYKSSSVIITEYAAQSFPAAVY
                     "
     gene            4437..4574
                     /locus_tag="PLS16_08235"
     CDS             4437..4574
                     /locus_tag="PLS16_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61108.1"
                     /translation="MNNFLKVKDEQNIKSERLTASFRSKLKALLAKINKKKDFSGLTD
                     E"
     gene            9536..9682
                     /locus_tag="PLS16_08240"
     CDS             9536..9682
                     /locus_tag="PLS16_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61109.1"
                     /translation="MELFTAVWVAYGYKFPIVGNVFPHDCVTPFEFGPLNLLAIVPAL
                     EGLG"
BASE COUNT         4300 a         1606 c         1829 g         3180 t
ORIGIN      
        1 acccccaacc ccaaccccaa ccccttttaa tttacatagt aaaattgatt ttcataaaaa
       61 ttaatatgtc atatacacaa aatattagac cttcacaata ttcattaggt ctattcactt
      121 gcatcaaatg ctaaaaattt cataatatct gggaaagagg atccttttca gcatgggaaa
      181 aacttttatc agaactcaaa aaaatagtgg agcttcagcc taatgaattt gtaaaagtaa
      241 cagaaattaa ttcaaaagtg atggcattaa tccatgaatt tattaagtaa taacatgacg
      301 gaaatccaag gagatattcc cgatccgctt tgaggacact gtaaaatact caatattatc
      361 attgtatggc tcaagcaagt ccaatgataa ctgtggaaaa catatattgc aacttaaggg
      421 atattggtga aagaatagca aaagaaagag gactgaaaga agatgttgac ccaaaataat
      481 ttaaagaaga tgtttgataa ttggatgttc ttgattataa tataatgaaa aagcccaaga
      541 cccgatatga acttccacca accattgtcg gaacaactgt agctaccaca agctacaaaa
      601 gttcaagtgt cataatcact gaatatgcag cacaatcgtt tccagcagca gtatattagt
      661 tttcttataa tgattaacaa gctgttgccg ttaacttaaa aggaatgacg ctgttgattt
      721 tggctaatgc aaattaaagt ttcgcaagtt tagccacatc aatgaggaat ttaacgctat
      781 tgtaaaactt taattataag tatttggtga ctcaacatgt tggtgttcca tattatttta
      841 ttcaaataaa tagaggaacc tttaatactg gattaggaac gtatttcgct aatttcggat
      901 atttatatca gaggaaacaa gatttctttt agaattaaat tccttattac atgggaataa
      961 tttcaaagta aggatttcta gtatttaatt ttgcttacca aaatctgatg agatcatctt
     1021 tttaaatgaa cataccgtct taacatcata aaaagatagt taatgaatgg gctgctagtt
     1081 agcacctaac gacatttatc cctaaaatct aaaagaaata tgaatgttgc atgaattaaa
     1141 atatcccgta atttaaattc tatgagcaat tcgcaacaat aaaagagatc aattctgaga
     1201 tactaaatta aaaagccaag gcagcgtaaa aggtcatgca aataaatgat tctataagcc
     1261 cagactataa gagacttatt gtagggtaat caatacaaaa ggcataaaat gttacgatag
     1321 cttcaagagc gtcacaattt attgatatga tgaaagaaat agcttctgtg ttaccttaat
     1381 aaactaaatc aggtcttcaa gagagatagc ctttacgatt aataactaga ttagtttcat
     1441 ccttcacaag ttattatagt atgtttcaaa ataccataat gcaacaagca aatttgggga
     1501 agagtcaacg tcaacaaaaa gcggcccatg ctagattcaa tgctggaatg gcctaatatt
     1561 ctagaaatgt ggcgttattg aatttggata tattcaaatg cttaagaaca cgaaatccta
     1621 atgatatcag gaacatggga tataatttaa gtaagatcca atagtttctg aaagatattt
     1681 gcgtgaatta cagactgact tatgatcatt agtttgaaaa taaggttcac aaatgtcttt
     1741 ttgcaattgg taaaatttta gaaatcacta aagatcgacc aatccctcgt ttcaaaagat
     1801 aacctccgaa aggaaagtaa agttctgttt aaactaccaa aatgaaatca taaatggaaa
     1861 caatgtcgaa gtaattggtt gcattaagta aaatgggtga gtaataagtt gctgttttat
     1921 tgacagtcga aaaaaaagtt agcttgtcag gtatataagt gaaggaagat attgccccaa
     1981 aagaataaaa ctttgctaca agtggaagtt atatgaataa ctaacctcaa atataataat
     2041 caatgtaata ataaagggac ccgaaaattc caaaataaat ttaaaaaagc aacttggtgc
     2101 ttgcaatcga aggttaaaaa ccgatgctca tgattgaaag ctaaagaaaa tatccgaata
     2161 tcaacacaaa ttagataact attgctgttc taccaagtta agttcaataa gccaagcaac
     2221 aggaatagca acttaaacat gtattagata tgtctgcttt agttttaatt taaaaaagag
     2281 tgaggcagct ttagttgatt gggcaaagaa tagcttatta taacgatctt attaaataag
     2341 gaaagattta tgatcccgtc acttgctata cggaattgga agcaaatgca gcacttgtaa
     2401 tcactctatc ataaggactc ttattttaga ttgatatttg tgctgaacag ttataacaaa
     2461 atgcctagaa tcaactcaga attcaattgt tgcgataaaa gctaaggatc gcgacgtctc
     2521 cttcgcttcg ctttacaatc acttctagca tattataaat acatagtgca caaacattag
     2581 aaatgagttc tgttttaaag gatagattag ttcatttaga tagaaaactt tttgattaag
     2641 atagccaggt gttgatctag ataatacttg aaatgtaagc ttctggaacc cgcgatacga
     2701 ccttaaatta aatcgtagat attataaaaa gttctaaata acctctgcaa aggattttag
     2761 aacgatacca cgagaaattt acagcgcata aatcagtttt agctgaaata tcagagaaga
     2821 taaaataaaa taaaagtaga atgatggctg ataacgacaa ttaggacaat gttaaagctt
     2881 atttaaacaa tataggagtg acttaactta aataacaagt caaataatta aaataaaatc
     2941 tcgcaaaaac aaatacgctt ggtatttcaa ggcaagtaca ataagcaata gctctgtaaa
     3001 atacttcaaa gtaaccaaca gaactttaag ttgcagcagc agctaaattg atgtcataag
     3061 cacataaatc aattatggga gttcctcaaa ccgattactt attaaatgct aaggacggaa
     3121 tgaatataac ttataatcaa cttaatcaag ttcaaaataa tacttacatg ggtaacttcg
     3181 ctactgtaca aggatatttc gattattggg gaggaatcca attccaacag acctctcaat
     3241 tggtccagtt aggagtatca gctcagatga tgttacagta acaatataat cagaccatcg
     3301 caaacagcaa ctaaataaaa atgatgtttt caattcttta tcaaagcaac caataaatat
     3361 taaattaagt tagagtaata aatgagagaa cgcaaaacat tgaataattg ataaggaatg
     3421 aaagtgataa attaatggag gagttatagc gtgtgagtcg gaaaggtaat aattctaaat
     3481 aatatgcttt tatcttataa aaaattagca cattcaataa caatcagcaa taaatcattt
     3541 ctgaactttc aagaactaat aaaggataat aaaaacaact attagcaaat gtgttgaaga
     3601 aaatcgatta aatggcgtca aacgccgttt aaaaccagac agacgtccta caagctgtca
     3661 tgcatgctca aaagtcgaca gatagactcc attctctcac agtcgctgga aacttataat
     3721 tagctaacat gttacaaata agtgaaggga cactttcaaa aatgaatttt agcgtcgaca
     3781 atttgactaa gatgcatatg ttagtggcaa ataatactta gcgaatagaa gatagaattt
     3841 cataattaac aaatcaatat atgacatcct caggtgaaca aaagtatgcc tatatgcaaa
     3901 tattgcaagg agttgctaac aacctactaa cattaaaata aatgtcagat aggcaagaaa
     3961 tcgcggccta agaagcaaga aattccaata aacaggtcta taatgctatc aaagctaatt
     4021 caaatttggt cgaccgagtt atatcagata tttcaacagt tgatgggaat atgaagaaat
     4081 atttcatgga tattgagcgt ggattataaa ggtattcaag tggcatgttg aattttattt
     4141 aacaatcgaa caggtacaat atgtccattt ttcaatatgt aaacgaacaa ctgactcatt
     4201 tagatcaatc cattcgctag tcccaagaac ttcaataaaa aaatatttaa taattatcca
     4261 atcaaatcaa tttcgttggc aacactcttc attttgaaaa cgaagaaatg aatcaaaaca
     4321 tcctcatgaa tagaagtata gccttagata ttcaaggcgt cgtcaaataa ctttaccatg
     4381 gacagcagag gtccacatca taaataattc aaaatttaaa tcagaatctc tcataaatga
     4441 ataatttttt gaaagttaaa gatgaacaaa atataaagag tgaaagattg acagcttctt
     4501 ttcgctctaa gctcaaggca ttattagcaa aaattaataa gaaaaaagat ttttctggtt
     4561 tgactgatga ataacttttg gattaagcga caaaatcctt tgcagaactt gaaaaacgta
     4621 attaaatcga tgctcaaata aaactgtaaa tcgcgaattt atatggtgtt ctcggcatag
     4681 accaagatta aagtaattaa ggaaaattga gaaaattaaa ggaagctaca gttcttctaa
     4741 agaatcaatt acattaaaat agcatgaagt tatgtgaaga aaaagctaaa gccaagtttt
     4801 tataaaagag ttcggaagaa accaaggaag ccctgaaaag gacagagcaa aaagttgagt
     4861 aaaaagataa ataagtgcgg aatataaata aataattagt taaattaagt cattaaaacg
     4921 gcttaagtca agggagaata taaggatatg aggctaatat tggattgtta gaaaaacaga
     4981 tttaaaattc acaaaaggca tttaaaagat aaatgtaagg gatgatgaag atgggtgaaa
     5041 aagtctaaga cgcactaaaa gatcgaacgt aatatcaaaa ataactgcta gaaaaagctt
     5101 aaaaacaaat gcaaaaacaa caacagttga gcgaagagaa aattaagcaa acaaaggaaa
     5161 tgtataaaga gttgcttgat gaaaccaaat aataaaagat aaaggataaa aatatgatat
     5221 aagctttaaa taaacaatta aaagatctga gccagaaatg gggtgcggca gatgctaaag
     5281 tgcaagaata ttagcgaaca atcagttaat taaaagaata tcatagtgaa ttaacggcaa
     5341 aataataata aaaacaaaag caaattgcat ctgttaccaa ggaacaacag aaatatgtgg
     5401 aaggcttaaa gaaagatgca gtgtaattac aaaaaactct gatatagtct gactggaaat
     5461 ctagatatga gaaattgtaa ttaagcacag accatgtatt agcgtcgaat gctaattaaa
     5521 ttaaaaaatt aaaggatgat ttaaaaaaaa tcacgtaata aaaaagattg gttgatcggg
     5581 aagtaaagaa agcgaacaag taaaaggaaa agacatcggg aacgcaatca cagttataat
     5641 ctgattattt caaagtgtgt taagaatttt aataattcaa aaaacgtaat ctccaataaa
     5701 gataaaatat tgtaacaact aaaaatcaat aattgaggaa gttaaccatg taaaaaaaag
     5761 ccgtcgaaga cagaatcgca gctgcttaag ggactataaa gggattaaat gattcaatag
     5821 attaatatta aaagagaatc caaggacatc taaaaaatga ataaacatac tctagagttg
     5881 ctaaataaga tctaggaaaa aaacaacaac ttctcaatga attaaatttt gagaaataaa
     5941 agaaaatact acttgaaaca gaattgaatg gaataaaact ttaattagat catgaaaggg
     6001 ctgctattta aagattaaat aaataaatca ataagttaac tgatgacatc acaaaattga
     6061 aaggtcaaca tcataattaa aaagaagaat tgaagcgtca aaacgacgca agatttactt
     6121 aattaaaaaa taagcataaa acatagctcg aggcagcgac gtaataaacc aagaaaattg
     6181 ttgaagataa cgccaaattt aacgcagccg aacaaaaaaa attattagat tcaatcaact
     6241 aagtaaaaaa acagaaataa actatagatg gtctaaattt gaggatctcc gcgagataag
     6301 gagctgcaat taatgcaggt ctaaatttgc aagaagtcgc tgacatcgtg gcgaaaattc
     6361 gcaaaaaaaa atctttgcaa atttataaaa aataaaaaac gaaataaaaa attagagcca
     6421 ttgcgggtct ctccagtttc tgggttgggc aataacttca tgaatatgat gcaaaactga
     6481 cccccgaact taagaaggaa ctcgaagctc ttgcaaaata gtattaataa agataaaaat
     6541 aattaattgc tctgagggct tcgacacaag atttgataaa aatgaaagat gatttaatca
     6601 aataaagaga cgaactcaaa taaaataatc agaccataac taattaactt aaaaaaaaag
     6661 aagactaaaa aaaataggca atataaaaat tattctaagc aaatcaataa atgagtctga
     6721 taactgagcg ttagaaaata ttaaaacaag aaaagtaagc tctagaaaaa aagatatcag
     6781 caatggaaca gcaggttaga aatcaataag gattgatttt acaacttcaa aataaataaa
     6841 ttgcgttaaa aaaaaatata agtgatttgg aacaagtaaa gactgaaata acccaacagc
     6901 gataagattt acgagcttaa cgagactcaa gtgcagcata agttcagaaa ctgagataaa
     6961 cattacttca aatggatcgc ttaaaaactt aaaagtttgc cctataaaaa gagaaagcct
     7021 aaattattaa taaattaacc caagcagaaa ctagactagc agtgtatcaa gaagcgatga
     7081 acaaagctga tagcggcact aaaaccatct aagaagaaaa tcgaagaatt atatcataga
     7141 attaaaatat tcggaaaaaa ttaaaactgg caaaaaatat ctaagaaaca tatgcgagtg
     7201 ttttgtcttt attggaatcc agaatactat aatattcaca aagcatccaa acaatctacg
     7261 aagatgacac tgtacttgga gtgggagagt aacaactcat aaatttttat aatcaagaaa
     7321 ttatcaaaac caaatagatc ataaaacaaa tgttagccca tttcaatgct atgaaagctg
     7381 acagaacgac ataagctggt tctttcaatt aatccaggaa tgtttcagat atgtccctcg
     7441 atattacaca catccaaggg gattttgctg aaggtatgta aaaataattt aattttcttt
     7501 agaaaaaaga agaaaaggat tttttaaaaa acaattttga aaagaaaaat taaacgaaat
     7561 aaaaaaaata tgaataaggt cagtatgccg atggaaagct tattggatca gagcttggaa
     7621 ctcattcaac tgtcatcagt ttcattctat aaatagttaa tgttatataa gaaactatgg
     7681 aaagacttat aggagcaagt aatatatgtt aaaaaggaat tcggaacttc ttatagatgg
     7741 atgtcataat gaaggatgaa tggaagcata atattgttat gtctttcgtc tcgattttta
     7801 gtgaaatata aatacaaatt ttacaaggcc ttgaaacatt agcaacccct ttttaagaca
     7861 tctaatccta tttcccacag gctacaaatc agcgaattat tgacgccttc ggtaaaatag
     7921 gatcacaatt gaaaggaatt actagttcaa tcgatgcatt gacggcataa ttataaaaaa
     7981 aagacaaata atatgatgca tgatcattca tccacgtcca aatgttttta ttccatcttt
     8041 tgtatatttt gttatccctg aacttcttgt gaatatatat aatgtctgat ctcgagggaa
     8101 aacgttttga gtgttacatt caaatatgac ttcaaaatta cttatcgatg atacattgat
     8161 cccatttaat acatttacat tgggtttgaa tggggtgtat gaaataatat atgctgcttt
     8221 gccgactact cgattctaat gaatccatgc cattcccatt gcagtgtcag cgttgattga
     8281 attagcatta aatgcgtaat tttgattatt caataacgat ggatcataga aagtgttggt
     8341 atttgttggg ttaaatggtc ggttatttat tgaaaagtta taagtattta tcctgggtaa
     8401 cacagatttt gtaaataaat aagaatatgt tgcgtatatg ttttacacaa aaaccgaatt
     8461 gtcatcatcg taattgtttg aaaatacgtt acctccattt cctttatatg gttgtggtgg
     8521 ccagtaatct acggcaattt tagaccaata ttttgtcaca ttatttgaca atctcgtgcc
     8581 ttttctacaa aaagaaaata tttggtagtc gtttgggaca aataaacaaa ccatttcatt
     8641 taaatgggtc gaattacatc cgactatata tgaattattt ggcggagttc cacctggaat
     8701 ctgatattga ttgctgagtg cccaaaagtt tgatattata gtgatttatc gattcaaagc
     8761 tgtctaaaga tagatatcaa cttctccgct aagaaagtga taggtctgta atctatattt
     8821 tattctctaa atgcagtaat tccttttttg catattggcc attccaatat tatcgaattc
     8881 acttacgtcg cagtacccag aagtgaacat cgcataagga gataatgtga tttataaaag
     8941 acatttttga agagcagcga gaggaatcag taagaattgg gaaggatcaa tcaacactcc
     9001 aataactcct gatatcaatg gaattttgaa ttttcttctc atgatgggaa tcttagttgg
     9061 caagaattct ccattataga tcatcaattg aggaactgta ggggatatac aaggctaaaa
     9121 agttcctcta gtaaaatagg gacaaaataa agaattaaat ccaaaattag ctgaattaat
     9181 tacgtgggtg tttgcggtat taggcccatc ataattaagt aataaaggca ttgtgtttcg
     9241 agcttgaaca ctgtaaggaa tcgaattatt gaagtagaaa gggaattgtc catttgaaag
     9301 attgttattc aataagagtt agtatgtcgg aatgctttgc gtcgcttaag ggaatttgtt
     9361 ttaaatatat ttcgcttgat attcagatct tatgttatta gcatatccct ttatcttaag
     9421 tgaagcgtaa attgcagacc aactgctata atcatctcca ccattcaaat tcgtccctgt
     9481 cgtaacaaca ttagaatttg taatatgacc ttttgccata caagccgcat tagtgatgga
     9541 attattcaca gcagtttggg ttgcttatgg atataaattc ccaattgttg gaaatgtgtt
     9601 tccgcatgat tgtgttacac cattcgaatt cggtccacta aatctactgg caatagttcc
     9661 tgcattggaa ggtttaggat aaggcatggt atcgaatgat ccatatccta agccctagac
     9721 ctctcttcct taacttataa gttcatcata cccgatatca tttaagtatg ttgcaagagt
     9781 atcatactct gtgattcttt aaatttatct attgtcgctg tataaaacag tttgacttat
     9841 aaaagattgt gccgagctat ctatctgcaa taatccaaca ggggtgtaag ttgaacttgt
     9901 tgatactgtt atctcaatat atgagcgata aggatcgaaa acacctttac ccaagttcat
     9961 gaaattgaag cgcactatgt cattgttact ccattgttta tttgatgaca tgggatccaa
    10021 ttaccattct ttataaactg atgggaatcc ttttggcaag atgattgggt atgcgattta
    10081 gtctggtaat ttttgttcga tcataaatat atattatcat taataattaa gcatccatct
    10141 ttgttaattt cggagcatcc cttttttctc gagagcacag gcctatttat gatacaaaat
    10201 aaataaaccc cttttttatt catctttgag atctaaatac ttatcatacc actcgctcaa
    10261 cgatttccaa ctcttgcaca atacatatta ttaaatctgc tttgggtctt gacaggtttg
    10321 tcttagtctt gcctcgttga cactttgcat tataatatga aaataaaagt ttataaaaca
    10381 aaaagacaaa aaaaaaaaaa agtcacacat aagaagaaag cacatcgccc aattcgcaag
    10441 aagttaaaac gcagcccata ggtttcagaa atattggaag cgattaaaaa ggattactaa
    10501 cgatatgtga aatagaaatc atttgcgtga tagtttgatc aaatatttat tcaacattga
    10561 tatgagcttc ggcgaggcct ttccttatcg cttcaataga ttgatatatg atgttataat
    10621 tgcttttttg taaaaagaat tcccatgttt tcgtaaggct tttgtcacat gatcgaattg
    10681 tttcttatga atgaattaat ccatattttt tttataatgc cacctaaaaa aaaaagagaa
    10741 ccgagaggac tataacactt aggcaagagt ttctaaaagt caagacactc gttcatggcc
    10801 ggttccggga tctcaatcaa cgatgtttct gcaatcagcc atataggtaa tgtgttagat
    10861 agtttaaagg atggagaaaa accaggataa aaataaaagt aagctattta ataac
//
LOCUS       DAOQCW010000085        10914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_2247_length_10914_cov_5.402615, whole genome shotgun sequence.
ACCESSION   DAOQCW010000085 DAOQCW010000000
VERSION     DAOQCW010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10914
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2247_length_10914_cov_5.402615"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="PLS16_08245"
     CDS             complement(<1..1005)
                     /locus_tag="PLS16_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNJ61110.1"
                     /translation="MNPNNFTIKTQEALAEAQQIAFDLKHAYLDTFHLLKAILNIDND
                     IVPFLFSKTNVSLKNVEQDIDQLLKNQPTVSGDGMKYPSQNLGQVLLKANSYLKELGD
                     EYVSIEHLLLSLLSVNDATSKILKQQGADETKLKQAIKELRKGSTVNNPSADMTYNSL
                     DKYAKNLNQLAQSNKLDPVIGRDDEIRRVLHILSRRTKNNPILVGEPGVGKTAIAEGI
                     AFRIIQGDIPENLKSKIIYSLDMGALIAGAKYKGEFEERLKAVIKEVIASDGEIILFI
                     DEIHTLVGAGGGGEGAMDAANILKPALARGELRAVGATTLAEYQKYFEKDKALERRFQ
                     K"
     gene            complement(1215..1925)
                     /locus_tag="PLS16_08250"
     CDS             complement(1215..1925)
                     /locus_tag="PLS16_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNJ61111.1"
                     /translation="MGRAFEYRKASKMARWDKMAKDFTRVGREIVIAVKAGGAMPENN
                     PMLRRAMQNAKSVQMPKDRVEAAIKRATSKDEKDYHEIVYEGMGPHAVAIVVETATDN
                     PVRTVANLRTIFNKNGGSMGTSGMHDFIFTRKGAFTIHANNIDKEELELELIDAGLDD
                     MEDGENDTLIIYALFQDFGSMQKALEDKGIEVIKSELQRVPNHTKELSEAEIDEVLEL
                     IDKLEQDDDVQHVFHNLA"
     gene            2030..2434
                     /locus_tag="PLS16_08255"
     CDS             2030..2434
                     /locus_tag="PLS16_08255"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNJ61112.1"
                     /translation="MKKQEYPGGTTAFKKLINENLNYPKEALENKVQGNVFVSYMVDF
                     DGTVKNIKVLKGIGFGCNEEAIRLVGLMKYAPQRNRGIKITNTFKIKIEFKIEEKVIP
                     KPQTIVSYSYNIVPKKSEDKALKKSYSYTITQ"
     gene            complement(2437..3348)
                     /locus_tag="PLS16_08260"
     CDS             complement(2437..3348)
                     /locus_tag="PLS16_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61113.1"
                     /translation="MKQFIYKILFFSCLITFGYVILYTIANNLNKSDNDYMAAIIDKH
                     ERIDSIKSPKIIFAGGSNLPFGIDSKMIQDTLHIPVINLGLHAGLGLDFMLNELQSCI
                     KNGDIVLISTEYFLDNGYYNLKKFTQKIYPKAQHYYTKNYLLEIQANIENTRNHLKSL
                     LKQKKKNEVSVYSRESFNTYGDIAMQLTILAPKNIKADHILHYHHWEGIQKLNDFNQF
                     AKTKNVKVFFVYPAYPESKYLKNKTAIAKLNQDMEKELQFKILGQPNDFIMSDSLFFD
                     TTYHLISKGRTARSKKIIDLLQKERLQ"
     gene            complement(3354..4604)
                     /locus_tag="PLS16_08265"
     CDS             complement(3354..4604)
                     /locus_tag="PLS16_08265"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNJ61114.1"
                     /translation="MCDSKNQSNRKFWFLLSIFINLGFLGLFKYYNFFIESFAEAIAH
                     FGLKINPWTIKVILPVGISFYTFHGLSYVIDIYKKRIVAERNFVDYAVFVSFFPLLVA
                     GPIERATHLLPQIKQRRVFDYSKAVDGMRQILWGLFKKIVIADNCAGFANQIFSNSAD
                     YSGSTLLLGALFFTFQIYGDFSGYSDIALGTARLFGISLLRNFAYPYFSRDIAEFWRR
                     WHISLSTWFRDYLYIPLGGSKGSNWMRIRNTLIIFIVSGFWHGANWTFIVWGALNALF
                     IMPSIIMKTNRNNLEIVAKGKKLPTLKELLQIKLTFGLTVFAWIFFRAEDIHHALAYC
                     SKILSTSIFSLPKFLDMTNALIILIVTAIFMLIEWIGREQEFALQTIGLKWNRCFRYL
                     FYLLITFAIFWFGGKEQQFIYFQF"
     gene            complement(4910..5350)
                     /locus_tag="PLS16_08270"
     CDS             complement(4910..5350)
                     /locus_tag="PLS16_08270"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ61115.1"
                     /translation="MITIRVAKADDLEQIAVLFDEFRKFYAQNSDIDGAKAYLKERMN
                     NNESIIFVAENEEKLIGFIQMYPTFSSVSMMKDMIMNDLYVFPEYRKKGIGKALLETA
                     KKFSKDNNYKGLWVETANDNPARSLYEHLGWEKDVSFVHYYWQE"
     gene            complement(5357..6340)
                     /gene="ftsY"
                     /locus_tag="PLS16_08275"
     CDS             complement(5357..6340)
                     /gene="ftsY"
                     /locus_tag="PLS16_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNJ61116.1"
                     /translation="MGIFDKIFKGKTNKSEEDLQVEEQELQVGLEKTKQGFFSKITKA
                     VAGKTEIDIEFLDELEEILISSDVSLPTTIKIIDRLEQRVSKDKYINTKDLNNILKNE
                     IANILAENNTKDIIDYTSFIDKKPYVILVVGVNGVGKTTTIGKLAYQFKKAGKSVLLG
                     AADTFRAAAVDQLTIWSERVGVPIIKQGMGSDPAAVAFDTVQAGLARDTDVIIIDTAG
                     RLHNKIGLMEELSKIKKVMSKVIPDAPHEILLVLDASTGQNALEQAKHFSSATQVTAL
                     ALTKLDGTAKGGVVISITDEFKIPIKYIGLGEKMEQLQTFNRQAFIETLFN"
     gene            complement(6423..6593)
                     /locus_tag="PLS16_08280"
     CDS             complement(6423..6593)
                     /locus_tag="PLS16_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 family protein"
                     /protein_id="HNJ61117.1"
                     /translation="MGKVSKNSKVDRGKNIGESKNMVKIIKAVKSAKGGSYAFKEKIV
                     HKDLLQQTLKDL"
     gene            complement(6600..6785)
                     /gene="rpmG"
                     /locus_tag="PLS16_08285"
     CDS             complement(6600..6785)
                     /gene="rpmG"
                     /locus_tag="PLS16_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNJ61118.1"
                     /translation="MAKKKGNRIQVILECTEHKASGMPGISRYITKKNRKNTPERLEI
                     KKYNPILKKVTVHKEIK"
     gene            complement(6798..7043)
                     /gene="rpmB"
                     /locus_tag="PLS16_08290"
     CDS             complement(6798..7043)
                     /gene="rpmB"
                     /locus_tag="PLS16_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNJ61119.1"
                     /translation="MARFCDLTGKGPISGNSVSHSNIKTKRRFLPNLQKKSFYIPEVD
                     RWIDLKVSTSAIRTINKKGIYAYLKELQKKGDIQIGL"
     gene            complement(7126..7199)
                     /locus_tag="PLS16_08295"
     tRNA            complement(7126..7199)
                     /locus_tag="PLS16_08295"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7163..7165),aa:Arg,seq:acg)
     gene            7545..7913
                     /locus_tag="PLS16_08300"
     CDS             7545..7913
                     /locus_tag="PLS16_08300"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNJ61120.1"
                     /translation="MKFFIKLLIKVATIILLAYALPTLGIDIKVDSTLDAVKLAIGLS
                     LLNIFVKPILKILSFPITCLTMGLFSFVISAAIVKIADVLLTGFSTSGALDGWLAALV
                     FGLSFSFISSIVESIIIDKD"
     gene            7930..8592
                     /locus_tag="PLS16_08305"
     CDS             7930..8592
                     /locus_tag="PLS16_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HNJ61121.1"
                     /translation="MNFESSTMLLDFDITQEIPVMEHFYTIQGEGFYAGTPAYFIRLA
                     GCDVGCHWCDVKESWTANKKQLISYKALINTIKKTPTEIIVITGGEPLMYDLSTLTSL
                     LKLNDYQVNIETSGSYLLSGNIDWICVSPKKFKVALPEVFTKANELKFIIFNKSDFEF
                     AEKYYQKFLELNPANEQKIICLLQVEWSVKEKMLPQVIEYVKAHPHWKISTQVHKYID
                     VP"
     gene            complement(8589..9719)
                     /locus_tag="PLS16_08310"
     CDS             complement(8589..9719)
                     /locus_tag="PLS16_08310"
                     /inference="COORDINATES: protein motif:HMM:NF002806.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HNJ61122.1"
                     /translation="MVYLDYNATTPVATEVLDKMLPYFSEKFGNASSSTHPFGWKAKE
                     AVDISRSIIAQSLGCIDEEITFTSGATESINTVLKGIFNLYHTKGKHIITSKTEHNAV
                     LDTCEFLKKKGADITYLDVDKNGIINIEDLKNAIRKDTILVAIMYVNNETGIIQNIKE
                     ISNITHQNKSIFFCDATQAYGKLPINVDDLGIDALCISGHKIYGPKGIGILYLRRKSP
                     RVIVEPLMHGGHHERGVRSGTINVPSIVGIGKAAELAYKNLANNHLKIEQLRNYLEDG
                     LKGIFKEKIQIIGEQANRIFNTSSVIFPMRSEHIIRHIKNDIAVSVGSACSSDKNLPS
                     HVLTAMGYKKEEALSSIRFSLGNDTTKEEIDFVLEQFSTINY"
     gene            9776..10174
                     /gene="mce"
                     /locus_tag="PLS16_08315"
     CDS             9776..10174
                     /gene="mce"
                     /locus_tag="PLS16_08315"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HNJ61123.1"
                     /translation="MKKIDHIGIAVKDLENSNLIFSKLFNREPFHEENIKSQKLIASF
                     FKLEDTKIELLYPTSEDSTVAKFLEKKGEGIHHVAFEVTDIYAEMERLKKEGFKPLSE
                     KPYIGALNKLVCFLHPKDTNGVLIELCQKQ"
     gene            complement(10229..10579)
                     /locus_tag="PLS16_08320"
     CDS             complement(10229..10579)
                     /locus_tag="PLS16_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNJ61124.1"
                     /translation="MAIKITEECINCGACEPECPNNAIYEGGLEWRMSDGTSVSEDYE
                     LMDGRIVGANDQNKPISNDIYFIVTDKCTECKGFHEEPQCAAVCPVDCCVPDEDHVDS
                     EEFLLARKKKLHGE"
     gene            10657..>10914
                     /locus_tag="PLS16_08325"
     CDS             10657..>10914
                     /locus_tag="PLS16_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61125.1"
                     /translation="MNINHYIQASQQLGEFLLSDNEELTAIIHKTSIYNSWFTIDNIH
                     KSLRNIAIEMLNADKLTTWLNNYLIDYTSIEPKKVGIVAAGN"
BASE COUNT         3614 a         1695 c         1566 g         4039 t
ORIGIN      
        1 tttttggaaa cgacgctcta aggctttatc tttttcaaaa tatttttgat actcagctaa
       61 agttgttgca cctactgctc tcaattcgcc tcttgctaat gcaggtttta aaatattggc
      121 cgcatccata gcaccttctc caccaccacc agcaccaact aaagtgtgaa tttcatctat
      181 aaataaaata atttccccat ctgatgcaat gacttctttg attacagcct ttaatctttc
      241 ttcaaactcg cctttgtatt ttgctccagc aattaaagca cccatatcta atgaatagat
      301 aatttttgac tttaagtttt caggaatatc accttgaata attctaaatg caataccttc
      361 ggcaattgct gttttcccaa caccaggttc tccgaccaaa attggattat tttttgttct
      421 tcgagataaa atatgaagca ctcttcgtat ttcatcatca cgaccaatta caggatctaa
      481 cttattagat tgtgctaatt ggtttaagtt ttttgcgtat ttatctaatg aattgtatgt
      541 catatcagca cttggattat ttacagttga gccttttctt aattctttaa tggcttgttt
      601 taatttagtt tcatcagcac cttgttgttt tagtattttg gaagttgcat cgtttacact
      661 tagcaaggat aaaagcaaat gttcgataga aacatattca tcacctaatt cttttaaata
      721 agaattggct tttagtaaaa cttgccctaa attttgtgat gggtatttca taccatcgcc
      781 agaaactgtt ggttgatttt ttagcaattg gtcaatatct tgctctacgt tttttagtga
      841 tacatttgtt tttgaaaata aaaagggaac gatgtcattg tcaatgttca aaatggcttt
      901 caataaatga aaagtatcta aataagcatg ctttaagtcg aatgcaattt gttgtgcttc
      961 ggctaatgct tcttgtgttt taatggtgaa attatttgga ttcatatctt tctttgtttt
     1021 cttaatgata acatcaaaaa atatgcaagt gttcagtttt taatatattt attgaaaagt
     1081 atgaaaatat ttcatatttt aatttaaaaa tatgacaaat aatctatatt gtaatttgca
     1141 aaatgccaaa aggtcagtta ttgacctgtt aaagtaaaat atgaccttta attttgaaag
     1201 aatgatgtgt gtttctaagc caaattatgg aaaacatgtt ggacatcgtc atcttgttcc
     1261 aatttgtcta ttaattctaa aacctcatca atttctgctt cagataattc tttggtatgg
     1321 tttggaactc tttgtaattc agatttaata acttcaatac ctttatcttc caaagctttt
     1381 tgcatagaac caaaatcttg gaataaagca tagattatca atgtgtcatt ttcgccatct
     1441 tccatatcat ctaagccagc atcgatgagt tctaactcta attcttcttt atcaatatta
     1501 tttgcatgaa tagtaaatgc tccttttcta gtaaaaataa aatcgtgcat tcctgaagtg
     1561 cccattgaac caccattttt attaaatatt gttcgcaaat tggcaacagt tcggacagga
     1621 ttatccgtag ctgtttcaac aacaatggct actgcatgtg gtcccattcc ttcatataca
     1681 atttcatggt aatctttctc atctttagag gttgcacgtt tgatagcagc ttccactcta
     1741 tcttttggca tttgtacact cttagcattt tgcatagctc tacgcaacat aggattgttt
     1801 tcaggcattg caccgccagc ttttacagca atgactattt cacgacctac tcgtgtaaaa
     1861 tctttagcca ttttatccca gcgtgccatt ttcgatgctt ttctatattc aaacgctctt
     1921 cccatagatt aatttttttt agtgcttttt tctatctaat tttagatgca aagataaata
     1981 actttaatcg ctataaaaag atatgtgtgc aaaaaagcaa aaggattttg tgaaaaaaca
     2041 agaatatcca ggcggaacta ctgcttttaa aaagctaata aacgaaaatc taaattatcc
     2101 aaaggaggca ttagaaaata aagtacaagg aaatgttttt gtaagttata tggtggattt
     2161 tgatggtaca gttaaaaata taaaagtatt gaaaggaatt ggctttggtt gcaatgaaga
     2221 agctattaga ttagttggtc tgatgaaata tgcaccacaa cgtaatcgtg gtattaaaat
     2281 tacaaatact ttcaaaatca aaattgagtt caaaattgaa gaaaaggtaa tacctaaacc
     2341 tcaaacaata gttagttatt cttataatat agttccgaaa aaatctgaag ataaagctct
     2401 caagaagtct tattcatata cgattactca atgaaattat tgtagtcttt ctttttgcaa
     2461 taagtcaata atttttttac ttcgtgcagt tctgcctttt gaaataagat gataagtagt
     2521 atcaaaaaat aaactatcag acattataaa atcatttggt tgtcctaaaa ttttaaattg
     2581 caattccttt tccatgtctt gatttagttt tgcaattgct gttttatttt ttagatattt
     2641 agattcagga taagctggat aaacaaaaaa tactttaacg tttttggttt ttgcaaattg
     2701 attaaagtca ttcagttttt gaataccttc ccaatgatga tagtgcaaaa tatggtcagc
     2761 ttttatattt ttgggagcga gaatagttaa ttgcatagca atatctccat acgtattaaa
     2821 gctttctctc gaatatacag aaacttcatt tttctttttt tgttttaaaa gtgatttcaa
     2881 atgatttctt gtgttttcga tattggcttg aatttcaagt aaataatttt ttgtataata
     2941 atgctgtgct tttggatata tcttttgagt aaattttttt aaattataat aaccattatc
     3001 taaaaaatat tcggtagaaa taaggactat atcaccattt ttaatgcagc tttgtagttc
     3061 gttgagcata aaatccaaac ccaaaccagc atgtaaacca agattaatta ctggaatatg
     3121 aagcgtgtct tgaatcattt tactatcaat gccaaatggt aaatttgaac ctccagcaaa
     3181 tataattttt ggcgatttta tactgtcaat tctttcgtgt ttgtcaataa ttgcagccat
     3241 ataatcattg tcagatttat tgagattatt tgcaatagta tatagtataa cataaccaaa
     3301 agtgataagg catgaaaaaa ataatatttt gtatataaat tgtttcatat tccctaaaac
     3361 tgaaaataga taaattgttg ttctttgcca ccaaaccaaa agatggcaaa tgtgattaat
     3421 aaataaaata aatacctaaa gcatctgttc cattttaaac ctatcgtttg taatgcaaat
     3481 tcttgttctc ggcctatcca ttctatcaac ataaaaattg cggttacgat taagataatt
     3541 aaagcgttag tcatgtctaa aaatttaggc agactgaata tggaagtaga taagattttt
     3601 gaacaatagg ctaaagcatg atggatatct tcagctctaa aaaatatcca agcaaaaaca
     3661 gtcaatccga atgttagttt aatttgtaat aactctttta gtgtaggtaa tttttttcct
     3721 tttgccacga tttctaaatt gttcctattg gttttcataa taattgaagg cataatgaat
     3781 aatgcattca atgcacccca aactataaaa gtccaattgg ctccgtgcca aaatccacta
     3841 actataaaga taattaaggt atttcttata cgcatccaat tactaccttt actgccacca
     3901 agcggaatgt ataaataatc tctaaaccaa gtagaaaggg atatatgcca acgtcgccaa
     3961 aattctgcaa tatctctcga aaaatatgga taggcaaagt ttctcaataa gctaattcca
     4021 aacaatcttg cagttcctag agcaatatct gaatagccag aaaaatcacc atagatttga
     4081 aaggtaaaaa ataaagcacc taacagcaag gtacttcctg aataatcggc agaattgctg
     4141 aatatttgat ttgcaaatcc tgcacaatta tcagcgatta ctattttttt gaataatccc
     4201 caaagtattt gacgcatacc atcaactgct ttagaataat caaaaactcg tctttgtttt
     4261 atctgtggta ataaatgtgt ggctcgctca attggacctg ccacaagcaa tggaaagaaa
     4321 ctcacaaata ctgcataatc aacaaaattt ctttctgcta caattcgctt tttataaata
     4381 tcaattacat acgataagcc atgaaatgta taaaatgaaa tgccaacagg taatattact
     4441 tttattgtcc aaggatttat ttttaaacca aaatgtgcta tggcttcagc aaatgattca
     4501 ataaaaaagt tataatattt gaatagccca agaaacccaa gattgataaa aatactcagt
     4561 aaaaaccaaa attttctatt gctttggttt ttagaatcgc acattttgag tcctgtgtag
     4621 aaatctaata aggtagaaaa taataataga aataaaaatc gccaatccca acaagcataa
     4681 aaaaagtaac tggcaatcag caataatgca ttttgagact ttaggttttt attaaataca
     4741 aaccaataaa atataaaaac gattggtaga aaaatggcaa aatcaataga gttaaaaagc
     4801 attggagtat tctttgtatt taatgatttt gcaactaaaa aattgtcaaa ttaaatataa
     4861 ttatgatgga tttaggctaa tcctcaaaat taaaaactaa caaaatgaat tactcttgcc
     4921 agtagtaatg aacaaaagaa acatcttttt cccaacctaa atgttcgtat aatgaacgag
     4981 caggattgtc atttgctgtt tctacccata aacctttgta attattgtct ttactgaatt
     5041 tttttgcagt ttctaataaa gcttttccga tacctttttt tctatattct gggaagacat
     5101 aaaggtcatt cattatcata tctttcatca tagaaacaga agagaaagtt ggatacattt
     5161 gaataaatcc aatgagtttt tcttcgtttt cagcaacaaa aatgatggac tcattattgt
     5221 tcattctttc ttttaaataa gcttttgctc catcaatatc agaattctga gcgtagaatt
     5281 ttctaaattc atcaaataaa actgctattt gttcaagatc atctgctttt gctactctaa
     5341 tcgttatcat tttgttttaa ttaaataatg tttcaataaa tgcttggcgg ttgaatgttt
     5401 gtaattgttc cattttttcg cccaaaccta tatattttat aggaatttta aattcatcag
     5461 taatactaat aacaacacca cctttagctg taccatctaa ttttgttagt gctaacgcag
     5521 ttacttgtgt tgcagaagaa aaatgtttag cttgttctaa agcattttgt ccagtcgaag
     5581 catctagtac caaaagaatt tcgtgcggag catctggaat aactttagac attacttttt
     5641 ttattttgga caattcttcc atcaaaccaa ttttattatg taatcgacct gcagtatcga
     5701 tgattataac atctgtatct cttgctaaac cagcttgtac cgtatcgaat gcaactgctg
     5761 ctgggtcgct tcccattcct tgcttgataa taggaacacc gactctttca ctccaaatag
     5821 ttaattgatc aacggcagct gctctaaaag tatccgccgc acccaacaaa acagattttc
     5881 ctgctttttt aaattgataa gctaatttac caattgtggt tgtcttacct acaccattaa
     5941 cgccaacaac taaaataaca taaggttttt tatcaatgaa ggaagtgtaa tcaataatat
     6001 ctttggtatt attttctgcc aaaatattgg caatttcatt tttaaggata ttgtttaagt
     6061 ctttggtatt gatatattta tctttagaga ctctttgttc taaacggtca ataattttaa
     6121 tagttgttgg taaactaaca tctgaagaga ttaatatttc ttctaattca tctaagaatt
     6181 caatgtcaat ttcagttttt ccagctacag ctttggttat tttagaaaag aaaccttgtt
     6241 tggttttttc taagcccact tgcaactctt gctcttcaac ttgtaaatct tcttcagatt
     6301 tatttgtttt tcctttgaaa attttatcaa aaatacccat tacacaatat aatcaagata
     6361 aagataataa aaaaaagctt ctcaaaatag agaagctttt taatttttta gatttaataa
     6421 tattataaat cttttaaggt ttgttgaagt aaatctttgt gaacgatttt ttctttgaat
     6481 gcataagaac cacctttagc tgatttaaca gctttgatga tttttaccat attcttagat
     6541 tcaccaatat ttttacctct atctactttg gagttttttg atacttttcc catgatttat
     6601 tattttattt ctttatgaac agtaactttc ttcaagatag gattatattt tttaatttcc
     6661 aatctttctg gagtgttttt tctatttttc tttgtaatgt aacgagaaat acctggcata
     6721 cctgatgctt tgtgttctgt acattctaaa ataacttgga ttctattacc tttcttcttt
     6781 gccatgatat gtatttttta taatccgatt tggatatctc ctttcttttg taattctttt
     6841 aaataagcat aaatgccttt tttgttgatg gttctgattg cagaagtaga aactttcaaa
     6901 tctatccatc tgtccacttc aggaatgtaa aaagattttt tttgcaagtt aggcaaaaat
     6961 cttctttttg ttttgatgtt tgaatgcgaa acgctgtttc ctgatattgg tccttttcct
     7021 gttaaatcgc aaaaacgtgc cataataatt cttttctttt tttaagagtt tgcaaatttc
     7081 cgacttttta ttgaaatatg caaatttatt tttattaata atttgtgaac ctggagagag
     7141 tcgaactccc aacctccaga gccgtaatct ggtgctctat ccaattaagc tacaggtcca
     7201 tttcggaacg caaagataat gcttttattt tattatttac tatcttagct tttatttaat
     7261 atggttttta tcaactaaat gttgatgaaa ttatcaggat atgtatttgt tatgtttaga
     7321 taaatcaaaa aaggcatttt ctaaagtgtt tcatctattt aatttatttt tgttagacgt
     7381 gttataaata aagtgattag tctggaaaaa tttacgcttt tatataaaat atactttaaa
     7441 gctaaatgtc gaacttgacg ttttcataat tactttctct tttttttttg aaataacatt
     7501 tgatattttt atcaattaca actatatttg ataaatattt tgatatgaaa ttttttataa
     7561 aattattgat taaggtagca accattattt tattggctta tgctctacct actttaggaa
     7621 tagatattaa agtggattca actttagatg ctgtaaaatt agctattggt ttgagtttgt
     7681 taaatatatt tgtaaagcca attttaaaaa tattatcttt tccaattact tgtttgacaa
     7741 tgggtttatt ttctttcgtc atcagtgctg caattgttaa aatagcagat gttctattaa
     7801 ctgggtttag tacttcaggt gcattagatg gttggttagc agccttagtt tttgggttgt
     7861 ctttttcttt tatcagttca atcgtagaaa gtattatcat agataaagat taataattac
     7921 ttttgtagct tgaattttga atcaagtacg atgttgttgg attttgatat aacacaagaa
     7981 attcctgtga tggagcattt ttataccatt cagggagaag gtttttatgc tggtacgcct
     8041 gcttatttca ttcgactagc tggttgtgat gttggttgtc attggtgtga tgtgaaagaa
     8101 tcttggacag ctaacaaaaa gcaattaata agttataaag cattaattaa tacaataaaa
     8161 aagactccaa ctgaaattat tgtaatcact ggaggtgaac ccttgatgta tgatttaagt
     8221 actttaactt cattgttaaa attaaatgat tatcaagtaa atatagaaac atctggaagt
     8281 tatcttttaa gcggaaatat agactggatt tgtgtttctc ctaaaaaatt taaagtggca
     8341 ttgccagaag tctttactaa agctaatgaa cttaaattta tcatttttaa taaatctgat
     8401 tttgaatttg cagaaaaata ttatcaaaaa tttttggaat taaatcctgc aaacgaacaa
     8461 aaaataattt gcttattaca agtagaatgg tcagtgaaag aaaaaatgtt accgcaagtt
     8521 attgaatatg ttaaagcaca tccgcattgg aaaatttcta ctcaagtaca taaatatatt
     8581 gatgtacctt agtagttgat agttgaaaat tgttctaata caaaatctat ttcttctttt
     8641 gttgtatcat ttcctaaact aaatcggata ctacttaatg cttcttcttt tttatagccc
     8701 atagcggtta atacgtgtga aggtaaattt ttgtcgctac tacaagcaga accaacagaa
     8761 acggctatgt catttttaat atgcctgatg atatgttcag aacgcattgg aaatattaca
     8821 gatgaagtat taaaaatacg attggcttgt tctccaataa tttgaatctt ttctttaaaa
     8881 atacctttta agccatcttc taaataattt ctaagttgct caattttgag atgattatta
     8941 gctaagtttt tataggcaag ctcggcagct tttccaatgc caacaatact aggaacgttt
     9001 atagttccac ttcgtacacc tcgctcatga tgtccgccat gcatcaacgg ttctacaatt
     9061 actcttggac tttttcttct taaatataaa ataccaattc ctttaggtcc ataaatttta
     9121 tgtccagaaa tgcataatgc atcaatacct aaatcatcaa cgtttatagg tagtttgcca
     9181 taagcttgtg ttgcatcaca aaaaaaaata cttttatttt gatgtgtaat gttgctgatt
     9241 tcttttatat tctggataat accagtttcg ttgttgacat acataatcgc aactaaaata
     9301 gtatcttttc taattgcatt ttttaaatct tcgatattaa ttataccgtt tttatcgaca
     9361 tctaagtatg taatatcagc accttttttc tttaggaatt cacaagtatc taaaactgca
     9421 ttgtgttcag ttttactagt gataatatgt ttgccttttg tatgatataa attaaatata
     9481 ccttttaaaa cagtattgat gctttctgtc gcacctgatg taaatgtaat ttcttcatca
     9541 atacaaccca aagattgtgc aataatgctt cttgaaatat ctacagcttc tttggctttc
     9601 caaccaaaag ggtgggtgct actcgatgca tttccaaatt tttcagaaaa ataaggcaac
     9661 attttatcta agacttcagt ggctactggt gttgttgcat tgtaatctaa ataaaccatt
     9721 gcttaaagtt ataaaatttg gaatatattt aataaattgt catcaaaaat aatagatgaa
     9781 aaaaatagac catataggta ttgctgttaa agatttagaa aattccaatt tgattttttc
     9841 aaagttattt aatcgtgaac cttttcatga agaaaatatt aaaagtcaaa aactgattgc
     9901 ttcttttttt aaattagaag atacaaaaat tgagttgtta tatccaacat cggaagatag
     9961 tacagttgct aaatttttag agaaaaaagg agaggggatt catcatgttg catttgaagt
    10021 tacagatatt tatgcagaaa tggaacgctt aaaaaaagaa ggttttaaac cattgtcaga
    10081 aaaaccatat atcggtgctt tgaataaatt agtctgtttt ttacatccga aagacacgaa
    10141 tggtgtttta attgaattgt gccaaaaaca ataaaaaaaa cgatggttaa agagtttaac
    10201 caccgttttc cttataaatt attgtagctt attcgccgtg caattttttc tttcttgcta
    10261 ataagaattc ttcagaatct acatgatctt catctggtac acaacaatct actgggcaaa
    10321 cagcagcaca ctgtggttct tcgtgaaatc ctttacattc tgtacattta tcagttacaa
    10381 taaaatagat gtcattacta ataggtttat tttgatcatt tgccccaact attctaccgt
    10441 ccattaattc ataatcttct gaaacacttg tgccatcact cattcgccat tctaaaccgc
    10501 cttcataaat tgcattgttt gggcattctg gttcgcatgc tccacaatta atacattctt
    10561 cagttatttt gattgccata atattcttaa ttttgtctga acaaatataa agtttgcttt
    10621 tataaaaacg aaaatattac taataaatta ccgttaatga atatcaatca ctatatccaa
    10681 gcctcacaac aattaggcga gtttttatta tctgacaatg aagaactcac tgcaataatt
    10741 cacaaaacga gcatttataa ttcttggttt acgatagata atattcataa atcattgaga
    10801 aatattgcta ttgaaatgtt aaatgctgat aaacttacaa cttggttgaa taattattta
    10861 attgattata cttctattga acctaaaaaa gtgggtattg tggcagcagg taat
//
LOCUS       DAOQCW010000086        10761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_2286_length_10761_cov_5.155987, whole genome shotgun sequence.
ACCESSION   DAOQCW010000086 DAOQCW010000000
VERSION     DAOQCW010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10761
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2286_length_10761_cov_5.155987"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PLS16_08330"
     CDS             <1..409
                     /locus_tag="PLS16_08330"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNJ61126.1"
                     /translation="ITNTSENLEDVGKAVVYLGEEQAVTGGHASIFKPSENILSKYFA
                     YFTQTSEFFNQKRKLAKGTKVIDVSANDLAKIKIPIPSLSEQERIVAILDKMDTLTNS
                     ISEGLPREIELRQKQYEYYRNRLLDFDKWKVEN"
     gene            422..784
                     /locus_tag="PLS16_08335"
     CDS             422..784
                     /locus_tag="PLS16_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNJ61127.1"
                     /translation="MENKKENIVQEKSFAFALQIIKLSKFLMNKKKEFVISKQVLRSG
                     TAVGALIREAEHAQSKADFIHKMNIALKEANETDYWIELLYQSNEIDINAYHSIQPDI
                     QELIKLLVSIVKKSKERQ"
     gene            821..3967
                     /locus_tag="PLS16_08340"
     CDS             821..3967
                     /locus_tag="PLS16_08340"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HNJ61128.1"
                     /translation="MKKNNSQFSTLNSQLYNAIAETNNFIVLDKYDKFNTLNEVPSSY
                     QSENALENEFIQDLINQGYENPQNLKTNQDLLANVRIQLQALNKVQFTDAEWQRFVEE
                     YLDKPSDNLLDKTRKIHDDYIYDFLFDDGHLQNIYLVDKTTIANNKVQVINQFEQVGK
                     HSNRYDVSILVNGLPLVQIELKKRGVAIREAFNQVHRYTKESFNTENSLFKYLQIFVI
                     SNGTDSRYFANTTKRDKNSFDFTMNWAKANNSLITDLKDFTATFFQKNTLLRVLLTYS
                     VFDTSNTLLVMRPYQIAATERMLWKINSSYNAKNWAKPESGGYIWHTTGSGKTLTSFK
                     AAHLATQLDFIDKVFFVVDRKDLDYQTMKEYQRFSPDSVNGSNSTQGLKNNLEKDDNK
                     IIVTTIQKLNNLMKTEGDLPIYQKQVIFIFDECHRSQFGEAQKNLKKKFKKYYQFGFT
                     GTPIFSENALGSETTASVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKSLETEI
                     DEKKLSAAENKEAFLHPARIKEISQYILQNFRIKTHRTRGGNTGFNAMFAVSSVDAAK
                     LYYEELNNLQNDTEKPLKIATIFSFAANEEQNAIGDIIDETFEPSAMDSSAKEFLSKA
                     INDYNAMFATNYSVDSEKFQNYYKDLAKRVKNKEVDLLIVVGMFLTGFDAPTLNTLFV
                     DKNLRYHGLIQAFSRTNRIYDANKTFGNIITFRNLEQHTIDAITLFGDANTKNVVLEK
                     SYKEYLEGFTDIVNGEAKRGYKELVKELNEKFLNPSEIVKEQDKKDFAKLFGEYLKVE
                     NILQNYDEFTHLKALQTIDKNDAAAIEAFKETHFVSDEAIAEMLNIDMLTERTVQDYR
                     STYNDIREWLRREKAGKEKEASTLDWDDVVFEVDLLKSQEINLDYILELLLEKNKQTK
                     DKDALIEEIRRIIRSSIGNRAKESLVVDFINATDLDSLEGKEDIIEAFYLFAQKQQQK
                     EVDELIESEALNKEAAKRYITTSLKKEYASENGNDLNEILPKMSPLNPQYLSKKQSVF
                     QKISAFVEKYKRIGGELL"
     gene            complement(3970..4659)
                     /gene="rsmI"
                     /locus_tag="PLS16_08345"
     CDS             complement(3970..4659)
                     /gene="rsmI"
                     /locus_tag="PLS16_08345"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNJ61129.1"
                     /translation="MNNLLESNKLYLVPTPIGNLEDITLRALKVLEQADVVLAEDTRT
                     SGVLCKHFNISTPLQAYHKDNEHKVVQNIIYQIQSGKKVALISDAGTPGISDPGFLLV
                     RTCLENNIEVECLPGATAFVPALVNSGFPCDKFVFEGFLPHQKGRQKRLLALVNEHRT
                     IIFYESPYRILKLLKELTEHFGAERSVSISRELTKKFEETYRGSVASALEHFEAKAPK
                     GEFVVVVKGKE"
     gene            complement(4652..5227)
                     /locus_tag="PLS16_08350"
     CDS             complement(4652..5227)
                     /locus_tag="PLS16_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61130.1"
                     /translation="MHTIEPYWKWRDIYQAELDELSPFFGQTYNEFEFTDKIYNYVIH
                     PQWDSFGSETLYVKLLYVNYKKHTAIIELIGEWNDCIGNDIMFFKRDFIEILINHGIT
                     KFVLIGENILEFFAEHEDYYQEWHDDIVAEGGWIIALNFREHIMEEMIQANIHHYIYF
                     FNDDEQMNWRKFKPHHLIDFLEQNKTKLLHE"
     gene            complement(5231..6079)
                     /locus_tag="PLS16_08355"
     CDS             complement(5231..6079)
                     /locus_tag="PLS16_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61131.1"
                     /translation="MILFADSGSTKTNWIVYNPETKNKTEISTLGINPIVHHHDDIIR
                     ILNDNIELTDLGASVKTIRFFGAGCSSELRNNIARNAMQSIFSDAEIFIDEDMIAAGI
                     AVCKNKEGIACILGTGSNSIYFDGEKWHNSNAGIGFILGDEASGAYFGKQILRDFLYG
                     LMPTDIIYHLQDKYQLKKDDIFYNVYKNVSPNRYLASFAPILTTYRQTDYVQNLMNEG
                     FDVFFKYHIACFDNYKNFPVGFVGSIATHFQQEIEIVAKKYEVELGNFIQRPIDELVQ
                     FYTQGK"
     gene            6198..6854
                     /locus_tag="PLS16_08360"
     CDS             6198..6854
                     /locus_tag="PLS16_08360"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HNJ61132.1"
                     /translation="MNRTALLVGATGLVGSELLQLLLADNYFSSVIILSRKTINIQHQ
                     KLKEIIVDFNQLENYASEINAQYIYCCLGTTIKKAGSQEAFKKVDYEYPLAIAQIAKK
                     NGAENFSIITALGADKNSSIFYNRVKGEIQDALIQLDFKQLQIIQPSLLLGERSEKRF
                     GENIGQKIAPIFNQLLIGGFKKYKAIEGKQVAKAMLFYSKQANPKVQILTNDLLLNVK
                     "
     gene            complement(6919..8664)
                     /gene="ilvB"
                     /locus_tag="PLS16_08365"
     CDS             complement(6919..8664)
                     /gene="ilvB"
                     /locus_tag="PLS16_08365"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HNJ61133.1"
                     /translation="MKNKLAMSVDENKQNQISGAEAVIRSLVAEGIDLIYGYPGGAIM
                     PIYDALWHYMDKINHVLTRHEQGATHAAEGYARVSGKPVVVFATSGPGATNLITGIAD
                     AVMDSTPMVCITGQVPKHLLGTDAFQETDVVGVTMPIVKWNYQVTTAAEIPSVIAKAF
                     FIANSGKPGPVLIDITKNAQFELMNWTAYQKCDSVRGYEAKPSVNKDKIDEAAALINQ
                     AKKPLILAGQGVGISKAREILEAVSTKTGIPVATTMQGLGTISPAHPNFVGIPGMHGN
                     YSVNIKMNECDVMIAVGMRFDDRVTGDVKKFGKQAKVIHIEIDPVEIDKVVKTEVGVL
                     ADAKEALEALLPLLNDNKHTEWINTFKELDKQEQEKVIQPAIHPNAGGIKMGEIMHLI
                     NEKTKGNAIIVPDVGQHQMYAMRYYDYNNTNSWVSSGGLGTMGFALPAAIGAQMAAPD
                     RTVVAIIGDGCFQMTIQELGTIWQNKLPIKTVIFNNNYLGMVRQWQQLFFDKRYSSVA
                     LTNPDFVAISKGFGIEATKVDLRANLSKALDIMFAHDGPYVLEVTCEKEENLFPMVPS
                     GAACSDVILEPTKKI"
     gene            8845..9786
                     /locus_tag="PLS16_08370"
     CDS             8845..9786
                     /locus_tag="PLS16_08370"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNJ61134.1"
                     /translation="MTISDSKLISGEATHYLAEKIAESYGQDLTPVEVMRFSDGEFEP
                     VIQESVRGSFLFCIQSTFAPSDNLMELLLLLDAAKRASAGYIVAVLPYFGLARQDRKD
                     KPRVAIGAKLVANLLVAAGAQRVMTMDLHAPQIQGFFDIPVDHLDASAIFAPYVKSLE
                     LEDLMFASPDVGSTKRARSYALQFNADLVICDKFRTKHNEIESMRLIGNVEGKNVILV
                     DDLIDTGGTLCTAANLIMENGAKSVRALITHPVLSGKAYERIEKSALQELVVCDTIPL
                     KQQSEKIKVLSVASLFAKAIRNAHEYRSINSLFIQKK"
     gene            9794..10384
                     /locus_tag="PLS16_08375"
     CDS             9794..10384
                     /locus_tag="PLS16_08375"
                     /inference="COORDINATES: protein motif:HMM:NF004132.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HNJ61135.1"
                     /translation="MQTITISGQARTEVGKKATKADRKNGNVPCVMYAGNEVVHFTST
                     MSSLRPLLFTPNFYKVIVEINGTTSEALLKDVQTHPVTDEILHLDFIKLVPGATIFAE
                     IPIKIVGTSAGVKEGGKLLVKVRKLKVRATPETLKDVIEVDITPLKLGQSIKVKDLPE
                     QGFQILNSASIPLASIEIPRALRSAATAAASAAKKK"
BASE COUNT         3931 a         1840 c         1718 g         3272 t
ORIGIN      
        1 aattacaaac acaagtgaaa atttagaaga tgttggaaaa gcagttgtct atttaggtga
       61 agaacaggca gtaactggag gacacgcttc aatattcaaa ccttctgaaa atattttaag
      121 taaatacttt gcttatttta ctcaaacatc agaatttttt aatcaaaaga gaaaattagc
      181 aaaaggaaca aaagtaattg atgtttcagc caatgattta gcaaaaataa aaattcccat
      241 tccttcctta tccgagcaag aacgcatcgt agcgatattg gataaaatgg atacgctcac
      301 caacagcatc agcgagggtt tgccacgaga aatagaatta agacagaaac agtatgagta
      361 ttaccgcaat cgtttactcg atttcgataa atggaaagtt gagaattgag aattgaaaat
      421 tatggaaaat aaaaaagaaa atattgtaca agaaaagtcg tttgcttttg cattgcagat
      481 aataaaactt tctaaatttc tgatgaataa aaaaaaggaa tttgttatca gcaaacaagt
      541 tttacgcagt ggcactgctg ttggtgcgtt aattcgtgag gctgaacacg cacaaagcaa
      601 agccgatttt atccataaaa tgaatattgc actcaaagaa gcaaacgaaa cagattattg
      661 gatagaattg ttgtatcaat ccaatgaaat tgatataaat gcatatcact caattcaacc
      721 cgacattcaa gagctgatta aattgctggt ttcaatcgta aaaaaatcaa aagaaagaca
      781 ataaaaagaa ataattctca attcttaaaa ggattttgcc atgaaaaaaa ataattctca
      841 attctcaact ctcaattctc aattatacaa cgccatcgca gaaacaaaca atttcatcgt
      901 actcgacaaa tacgataaat tcaatacatt aaacgaagtt ccgtcttcct atcaatccga
      961 aaacgcactt gaaaatgaat ttatccaaga tttaattaat caaggctatg aaaatccgca
     1021 gaatttaaag acaaaccaag atttgttggc taatgtgcgt attcaacttc aagcattaaa
     1081 caaagtgcaa tttaccgatg cagaatggca acgttttgta gaagaatatt tggataaacc
     1141 cagcgataat ttgctggaca aaacccgcaa aattcacgat gattatattt acgatttctt
     1201 gtttgatgat ggtcatctcc aaaacattta tttggtggac aaaaccacaa ttgccaacaa
     1261 caaagtacag gtcatcaatc aatttgaaca agtaggcaaa catagtaatc gttatgatgt
     1321 aagcatttta gtaaacggtt tgcctttggt gcaaatcgaa ctcaaaaaac gaggcgtagc
     1381 cattcgtgaa gcctttaacc aagtgcatcg ctacaccaaa gagagtttca ataccgaaaa
     1441 ttcgctgttc aaatatttac aaatcttcgt gattagcaac ggaaccgaca gccgttactt
     1501 tgccaatacc accaagcgtg ataaaaacag tttcgatttt acgatgaatt gggcaaaagc
     1561 aaacaattcc ctgattaccg acctgaaaga tttcacagct actttctttc aaaaaaatac
     1621 cttgttgcgt gtgctactca cctattctgt atttgatacc agcaatactt tgttggtgat
     1681 gcgtccgtac caaattgctg caacggaaag aatgttgtgg aaaataaatt cttcttacaa
     1741 tgccaaaaac tgggcaaagc cagaaagtgg cggatatata tggcacacca caggttccgg
     1801 aaaaacctta accagcttta aagccgctca cctggctaca caattggatt ttattgataa
     1861 agtgtttttt gtggtggata gaaaagattt ggattatcaa actatgaaag aataccaacg
     1921 tttttcgccc gatagtgtga atggttctaa cagtacacaa ggtttgaaaa ataatttgga
     1981 aaaagacgat aacaaaatca tagttaccac cattcaaaag ctcaacaacc tgatgaaaac
     2041 cgaaggcgat ttgcctattt atcaaaagca ggtaattttt atttttgatg aatgtcatcg
     2101 cagtcaattt ggtgaagcac aaaaaaacct gaaaaagaaa tttaaaaaat attatcaatt
     2161 tggttttact ggtacaccta ttttttccga aaatgcttta ggctcagaaa ccaccgccag
     2221 tgtatttggt cgtgaactgc actcatacgt cattaccgat gccattcgtg atgaaaaagt
     2281 attgaaattt aaagtagatt acaatgatgt tcgaccacag tttaaaagct tggaaactga
     2341 aattgatgaa aagaaactca gtgcagccga aaacaaagaa gctttcttgc atccagcacg
     2401 tatcaaagag atttcacaat atattttaca aaatttcaga ataaaaactc accgcactcg
     2461 aggaggcaac acaggtttca atgctatgtt tgccgttagc agtgtagatg ccgcaaaatt
     2521 gtattacgaa gagttgaaca acttgcaaaa tgatactgaa aaaccgttaa aaattgccac
     2581 cattttttcc tttgctgcca acgaagaaca aaatgccatt ggcgatatta tagatgaaac
     2641 ctttgagccg agtgcaatgg acagcagtgc taaagaattt ttgagcaaag cgattaacga
     2701 ttacaacgcc atgtttgcca ccaattatag tgtggacagt gaaaaatttc agaactatta
     2761 taaagacctt gccaaacgag taaaaaataa agaagtagat ttgttgatag tggtgggcat
     2821 gttcctcact ggttttgatg cacctacctt aaataccttg tttgtagata aaaacctgcg
     2881 ttaccacggt ttgatacaag ccttttcaag aaccaataga atttatgatg ccaacaaaac
     2941 ctttggaaac atcatcactt tccgaaattt ggaacaacac accatagatg ccattacgct
     3001 ctttggcgat gccaatacca aaaacgtagt tttagaaaaa agctacaaag aatatttaga
     3061 aggatttacg gatattgtca atggtgaagc caaaagaggt tataaagagc tagtaaaaga
     3121 gttgaacgaa aaattcctta acccatctga aattgtaaaa gaacaagata aaaaggactt
     3181 tgcaaaattg tttggcgaat atttgaaagt agaaaatatc ttgcagaatt acgatgaatt
     3241 tacccacttg aaagccttgc aaaccattga caaaaacgat gcagcagcca tagaggcgtt
     3301 taaagaaacc cattttgtaa gcgatgaagc catagcagaa atgttaaata tagatatgct
     3361 aacagagcga acggtgcaag attatcgttc cacctacaac gacattcgag aatggttgcg
     3421 aagagaaaaa gcaggcaaag aaaaggaagc ctcaacatta gattgggacg atgtagtgtt
     3481 tgaagtagat ttattgaaat cgcaagaaat caatctggat tatatcttgg aactcttatt
     3541 ggaaaaaaac aagcaaacga aagacaaaga tgctttaatc gaagaaattc gtagaatcat
     3601 tcgttctagt ataggaaacc gtgccaaaga gagtttggtg gtggacttta ttaatgccac
     3661 agatttagat agtctggaag ggaaagaaga tatcatagaa gcattctatt tatttgcaca
     3721 aaaacaacag caaaaagaag ttgatgaatt gatagaaagc gaagcattga acaaggaagc
     3781 agccaaacgc tacattacca cttccttaaa aaaagaatac gccagtgaaa atggcaacga
     3841 tttgaatgaa atattgccaa agatgagtcc gttaaatccg caatatttaa gtaagaaaca
     3901 aagtgtcttt caaaaaatat cagcctttgt agagaagtat aaaagaattg gaggagaatt
     3961 attataagcc tactccttcc cttttacaac gactacaaat tcgccttttg gggctttagc
     4021 ctcaaaatgt tccaatgctg atgcaacaga accacgataa gtttcttcaa atttttttgt
     4081 caattctcta gaaatagaaa ccgaacgttc agcgccaaaa tgttcagtta attctttaag
     4141 tagctttaaa attcgataag gcgattcata aaaaataatg gttcgatgtt cgttaactaa
     4201 agccaacaat cttttttgcc tacctttttg atgtggaaga aatccctcaa atacaaattt
     4261 atcacaaggg aaaccagaat ttactaatgc aggtacaaaa gcagttgcac caggtaaaca
     4321 ttctacttca atattgtttt caaggcatgt acgcactaat aaaaaaccag gatctgaaat
     4381 gccaggcgtt ccagcatctg aaatcaaagc cacctttttg ccactttgaa tttgatatat
     4441 aatattttga acaactttat gttcgttgtc tttatgatag gcttgtaaag gcgttgaaat
     4501 attaaagtgc ttacacaaaa cgcctgaagt ccgagtatct tcagccaaaa caacatctgc
     4561 ttgttccaac acttttaacg ctcttaaagt aatatcttct aaattaccta ttggagttgg
     4621 tactaaataa agtttattac tttctaacaa attattcatg cagtaatttt gttttatttt
     4681 gttctaaaaa gtcaattaaa tgatgtggtt taaatttacg ccaattcatt tgttcatcat
     4741 cattaaagaa ataaatataa tgatgaatat tggcttgtat catttcttcc ataatatgtt
     4801 ctctaaaatt aagtgctatt atccaaccgc cttctgcaac aatatcatca tgccattctt
     4861 gataataatc ttcatgctct gcaaaaaatt ctaaaatatt ttcgccgatt aaaacgaatt
     4921 tagtaattcc atgatttatc agaatttcaa taaaatctcg tttaaaaaac ataatatcat
     4981 tgccaataca atcgttccac tcgcctatca attcaataat agccgtatgt tttttataat
     5041 tgacataaag caatttgaca tacaaagttt cagaaccaaa actatcccat tgtggatgaa
     5101 ttacataatt ataaatttta tcagtaaatt caaattcatt ataggtttgt ccaaaaaaag
     5161 gtgataattc atcaagctct gcttgataaa tatctcgcca tttccaatat ggttctattg
     5221 tatgcatggt ctattttcct tgcgtataaa actgtaccaa ttcatcaatt ggcctttgaa
     5281 taaaattccc taattctact tcatactttt tagcaacaat ttcaatttct tgttgaaaat
     5341 gcgtagcaat tgagcctaca aaacctacag gaaaattttt ataattatca aaacatgcaa
     5401 tatgatattt aaagaaaaca tcaaaacctt cattcattaa attttgaaca taatccgttt
     5461 gtctatacgt tgtaagtatt ggtgcaaaac tagctaaata acgatttggc gatacatttt
     5521 tatagacatt ataaaagata tcgtctttct tgagttgata tttatcttgc aaatgataaa
     5581 taatatctgt tggcatcaga ccatataaaa aatctcgtaa aatttgtttt ccaaaataag
     5641 ctccacttgc ctcatcacct aaaataaatc caataccagc atttgaatta tgccactttt
     5701 cgccatcaaa ataaatagaa ttagaacctg ttcctaaaat acatgctatc ccttctttgt
     5761 ttttacacac tgcaattcct gcggctatca tatcttcatc tataaaaatt tctgcatcag
     5821 aaaaaataga ttgcatagca tttcttgcaa tattatttct aagttcactt gaacaaccag
     5881 caccaaaaaa gcgtatagtt tttacacttg ctcccaaatc cgtcaattca atattatcat
     5941 tcaaaatacg aataatatca tcgtgatgat gtacaatagg gttaattcct aaggtgctta
     6001 tttctgtctt gtttttcgtt tcaggattat aaacaatcca atttgtttta gtagagccac
     6061 tatcagcaaa taaaatcata aaatcaaaag attaaaaaat gcaagatagg aaatatataa
     6121 acaattttta aaacaaaacg ttaccaaaca atacattccg cttaatataa ttatatttga
     6181 gataaattaa aaagaagatg aatagaactg cattattagt cggtgcgaca ggcttagttg
     6241 gctctgaact gttacaactt ttgttggccg ataattattt ttcaagtgta attattttga
     6301 gtaggaaaac tataaatata caacaccaaa agcttaaaga aattattgta gatttcaatc
     6361 aattagaaaa ttatgcttcg gagattaatg ctcaatatat ttattgttgc ttaggaacta
     6421 ccataaaaaa agcaggctct caagaagctt ttaaaaaagt agattatgaa tatcccttag
     6481 caattgcaca aattgctaaa aaaaatggag ctgaaaattt tagtatcatt acagcattag
     6541 gagctgataa aaattcgtct attttttaca atagagtaaa aggagaaatt caagatgccc
     6601 ttattcaatt agactttaag caactgcaaa ttatacaacc aagtttatta ttaggcgaac
     6661 gttctgaaaa aaggtttgga gaaaatatcg gacaaaaaat agctccaatt ttcaatcaat
     6721 tactaattgg tggctttaaa aaatataaag caatcgaagg taaacaagtt gccaaagcaa
     6781 tgctatttta cagcaaacaa gctaacccca aagtacaaat tttgactaat gatttattat
     6841 tgaacgtaaa ataaaacaag gcctaaagaa aaagcctcat tgttggacaa tgaggcttta
     6901 aacttctatt taaaaatatt aaattttctt agtaggttct aaaattacat cgctacaagc
     6961 agcaccactt ggcaccattg gaaacaaatt ttcttctttt tcgcaagtaa cttctaatac
     7021 ataaggacca tcgtgtgcaa acataatatc taaggctttg cttaaatttg ctctcaaatc
     7081 tacttttgta gcttcgatac caaacccttt agaaatagcc acaaaatcag gattggttaa
     7141 tgcaactgaa gagtatcttt tatcaaagaa taattgttgc cattgacgta ccatacctag
     7201 gtaattatta ttaaaaatta ctgttttgat aggtaattta ttttgccata ttgtacctaa
     7261 ttcttgtatc gtcatttgga aacacccatc gccaataatt gctactacag ttctatctgg
     7321 tgctgccatt tgagcaccaa ttgcagcagg caacgcaaag cccatagtac ctaaaccgcc
     7381 actacttacc catgagtttg tattgttata gtcatagtat cgcattgcat acatttgatg
     7441 ctgtccaaca tcaggtacaa taattgcatt gcccttagtt ttttcattta tcaaatgcat
     7501 aatttcaccc atttttatac ctccagcatt tggatgaata gcaggttgaa tcactttttc
     7561 ttgttcttgt ttatctaatt ctttaaaagt attaatccat tctgtatgct tattgtcatt
     7621 caataaaggt aataaagctt ctaaggcttc ttttgcatcc gccaaaacac ccacttcagt
     7681 tttaacgact ttatcaattt ccactggatc aatttcaata tgaattactt tggcttgttt
     7741 accaaatttt ttcacatcac cagttactct atcgtcaaaa cgcataccca cggcaatcat
     7801 aacatcacac tcattcattt tgatattcac tgaataattt ccgtgcatac caggaatccc
     7861 tacaaaattt ggatgtgcag gactaattgt acctaagcct tgcatggttg tcgcaacagg
     7921 aataccagtt ttggttgaaa cagcctccaa tatttcacga gctttagaaa ttcctacacc
     7981 ttgaccagct aaaatcaaag gttttttcgc ttgatttatt aaagcagcag cttcatcaat
     8041 tttatcctta tttacactag gttttgcttc ataaccacga acactatcgc atttttgata
     8101 agcagtccaa ttcattaact caaattgggc atttttggta atatctatca aaactggacc
     8161 aggttttcca ctatttgcaa taaaaaaagc tttagcaatt acacttggaa tttcagcagc
     8221 agtagttact tgataattcc atttaacaat tggcattgta accccaacca catcagtttc
     8281 ttgaaaggca tcagttccta ataaatgttt aggcacttga cctgtaatac aaaccattgg
     8341 cgtagaatcc attactgcat ctgcaatacc tgtaattaaa tttgtagctc caggtccaga
     8401 cgttgcaaaa acaacaacag gttttccact tacacgagca taaccttctg ctgcatgtgt
     8461 tgctccttgt tcatgtcttg ttaaaacatg attaatttta tccatataat gccaaagagc
     8521 atcataaatg ggcataattg caccacctgg ataaccataa attaaatcaa taccttcggc
     8581 tactaatgaa cgaataacgg cttctgcccc agaaatctga ttttgcttat tttcatcaac
     8641 tgacattgct aacttgtttt tcacaaatgt aacgattttt aatgggtcaa aaaataaatt
     8701 aaaaataggt atagattatt taatattatt caaaaccaag gattaaaaaa tcaattgcaa
     8761 agagaaaaat agcgtaaaca ttaattaatt ttatctatga taggaattag aaaaaaagac
     8821 actatctttg caaaaaatat agcggtgact atttcagatt ccaaattaat ttcaggcgaa
     8881 gcaacacact atttagcaga gaaaattgca gaaagttatg gtcaggactt aacacctgtt
     8941 gaagttatgc gattttcaga tggagagttt gagcctgtca ttcaagaatc ggtaagaggt
     9001 tcatttttat tttgtattca atcaacattt gctccatcag ataacttaat ggaactatta
     9061 ttgttgttag atgctgcaaa aagagcttcc gctggttata tcgttgctgt attaccttat
     9121 tttggtttag ctagacaaga cagaaaagac aaacctagag ttgctatcgg tgcaaaattg
     9181 gtagccaatt tattagttgc agcaggtgct caaagagtaa tgactatgga tttacatgct
     9241 cctcaaattc aaggtttttt cgatattcct gtggaccatt tagatgcttc tgcaattttt
     9301 gctccttatg taaaaagttt agaattagag gatttgatgt ttgcatcacc agatgtaggc
     9361 agcaccaaaa gagcaagaag ttatgcttta caatttaatg cagatttagt tatttgcgat
     9421 aaatttagaa ctaagcataa tgaaatcgaa tcaatgcgtt tgattggaaa tgtagaagga
     9481 aaaaatgtaa ttctagttga tgatttaatt gatactggag gaacattatg tacagcagcc
     9541 aacttgatta tggaaaatgg tgctaaaagt gtaagagctt taattacaca tccagtatta
     9601 tcaggaaaag cttacgaaag aatagaaaaa tcagcattgc aggaattagt ggtttgcgac
     9661 accattccat taaaacagca aagcgagaaa ataaaagtac tttctgttgc atcacttttt
     9721 gctaaggcaa ttagaaatgc acatgagtac cgatcaatta attcattatt tattcaaaaa
     9781 aagtaaaaaa attatgcaaa caattaccat tagcggacaa gctagaaccg aggtaggaaa
     9841 aaaagccaca aaagcagaca gaaaaaatgg caatgtacct tgtgttatgt atgcgggaaa
     9901 cgaggtggtg catttcacat ctacgatgag ttcattgcgc ccgttgttat tcacgccaaa
     9961 tttctacaaa gttatagtag aaatcaatgg aacaacatct gaagcattat tgaaagatgt
    10021 gcaaacacac cctgtaactg atgaaatctt acacttagat tttataaaat tagtaccagg
    10081 tgcaacaatt tttgctgaaa ttccaattaa aattgttgga acatctgcag gtgttaaaga
    10141 aggtggtaaa ttattagtta aagttagaaa actaaaagtt agagcaactc cagaaacatt
    10201 aaaagatgta atagaagtag atattacacc tttaaaatta ggtcagtcaa ttaaagttaa
    10261 agacttacct gaacaaggtt ttcaaatttt gaactcagct tcaataccat tagcttctat
    10321 tgaaatacca agagcattaa gaagtgcagc aactgcagca gcaagtgcgg ctaaaaagaa
    10381 ataaaaaatc ttttattata ttcaaaaaag tcctccaaaa tggaggactt tttttgtaat
    10441 actttagtca taatattttt taaaaaaaaa tacaaagtat tgtatatata aataatttta
    10501 tttaatattg cattaactaa atatttaaat tatgaaaaca ttaaaaagaa ttatggtaat
    10561 ttcattacta taatagcatt agcacaaaat gcttgtaaaa aagattgtga tgaggtaaac
    10621 atcaaaaatc aatatttaaa gcagcttaga gttcttgaag ataatataga taaaatttgg
    10681 agtgaaaatg gtccttcatg caatgacgtt aatactgaat tacaaaaact caaagacgag
    10741 tgtggtaata aagacgtttt c
//
LOCUS       DAOQCW010000087        10673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_2312_length_10673_cov_5.148616, whole genome shotgun sequence.
ACCESSION   DAOQCW010000087 DAOQCW010000000
VERSION     DAOQCW010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10673
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2312_length_10673_cov_5.148616"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2177
                     /gene="sprA"
                     /locus_tag="PLS16_08380"
     CDS             <1..2177
                     /gene="sprA"
                     /locus_tag="PLS16_08380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HNJ61136.1"
                     /translation="LSFRTDLNRQFNKTKIRDIGDDGLVLDPTYFKYFTWDRFWSIRY
                     NLTRSISVDYTANQQARIDEPFGELNTKAKRDSMWHNFLKLGRPTNYNHNFNASYNLP
                     LQHIPFLDWMTVRTRYGSTYQWTAPSLALQSLGAIAQNSMTYQINADVNFKNLYNKAK
                     FLKPYNTNQPKKTKEEYTKAVEQYEKQNQNFIDKMVQKEKEIEKKIEDIEAAKLDTTI
                     TREQIEQMVVEKKELKNQLRSLKYSKKGLTQPANPKLDFVVRPLMMLQRASLSLDRTY
                     TTVLPGLMPTPLLFGQNFEHKAPGFKFLMGGQRDTTWLGRKGEQGWISADTTLNYQFI
                     QTKQKTFNLRVSLEPFRDLRIDITMQKTQGENYSEFFKKTGNGMAYQHLTPQVSGNYS
                     ISFLMFRTIFGKVDDNNFTAAFRNFEKNRELYSQKFGDKNPNSTGIYINDSIVLPNYK
                     EGYGPFSQDVLLPSLIAAYTGKSIDRVKLNPLKTMPLPNWRLTYNGITKTKWGKKMFT
                     SFNITHGYNSTFSIGSYITNLNFLNTPGYVNEDMYYTPYNMDTLTGNYYSLYVVPQVA
                     IQEQLSPLIGLEATWKNSLITNFEFKKSRMLSLSMLDYRLSETRTTEITAALGYKIAH
                     FKVPFKIKGKKITLNNDINLRADFSIRNDKTVNYRLDQNIAEPVRGQKTMSLAATIDY
                     IVNEKFNIRVFYDFRKILPATLSSYPTKTHRGGVTFRFSLAQ"
     gene            2240..2617
                     /gene="gcvH"
                     /locus_tag="PLS16_08385"
     CDS             2240..2617
                     /gene="gcvH"
                     /locus_tag="PLS16_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNJ61137.1"
                     /translation="MQFPSDLKYSKEHEWVKVEGDTATIGITEFAQKELGDIIYVDIE
                     TLGEVIEQNGIFGSVEAVKTVSDLFMPIAGEIIEINALLADQPELVNSAPYGDGWMVK
                     IKITDASQVESLLDSEAYQALLG"
     gene            2622..4118
                     /locus_tag="PLS16_08390"
     CDS             2622..4118
                     /locus_tag="PLS16_08390"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNJ61138.1"
                     /translation="MFDKITKTIPAAIWTAMILYLCFLPGEKIPKDEFLQKIFFDKFV
                     HFVFYYTLFKFLQFAGWIDKKSSIIICVVLGLFVEIIQHFYIPNRHFSLGDIAVNILG
                     CGLAYYFTSEKKEEEPSNKTGIYFKNLDALRAIGCWYVTFVHMLGVYSVKTSANLPVQ
                     KGSLVEQFGQGFLTVFFVLSGFLISYFLIKEKTKTNKIAFKKFYIRRAFRILPIYLVG
                     LFLAFGFYKLVCAKGAPGLENGMDYCNSIDKIFLLYLFMIPNVAYSIGAFFPHISNYW
                     SVGVEEQFYIAWPIVLHKTKNILKSFAVIYIGYFIVLLITLVLYQFYKTDNYYKFFDF
                     VHHARFGAFAGGGFLAYIIINLEAYKSKYPKVYLFLISKKTQIASILLLLLAAYINNI
                     YFYAFKHVFIIIPCVFIILFNMINDDNNIFKIDNKFLNYVGLTTYSMYILNQIFLDVF
                     VGMGIGIFGAKNFIMVPLFSMGMLTLVSIASYKYIELTFMKIRGKYFA"
     gene            complement(4115..4852)
                     /locus_tag="PLS16_08395"
     CDS             complement(4115..4852)
                     /locus_tag="PLS16_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61139.1"
                     /translation="MFLRYTNRKHFRGFARRKKKLISLLEQYKIRRESKKVSVPQQII
                     SEKLKEVHKLETNLNNIKSNEQTSNIIQPTLQNSTTTISNNTSVQQGTKPKKILAQKS
                     SFGNLLKDLKEETIEAQKKYEFSQENAIELWKLFIEHIRPKAQNSFINIINNQVPEIK
                     GNTISLYVSNNINYEIIQIYCSDIVSFFISNTNNNRIELDIIVQKNEQDDNAPKKKPV
                     DKLKELIDKNDKIVYLIEKLSIDLEAF"
     gene            complement(4789..5928)
                     /gene="dnaX"
                     /locus_tag="PLS16_08400"
     CDS             complement(4789..5928)
                     /gene="dnaX"
                     /locus_tag="PLS16_08400"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNJ61140.1"
                     /translation="MDNFIVSARKYRPMRFTDVVGQEQVSETLKKALSSGKLAQSFLF
                     CGPRGVGKTTCARILAKAINCENPSEEFEPCNECNSCKSFTQNASFNIFELDAASNNS
                     VDDIRVLVDQVRFAPQAGKYKVYIIDEVHMLSPGAFNAFLKTLEEPPSYAIFILATTE
                     KHKIIPTILSRCQIFDFKRIANHDIVKQLQYIADSEQIETEDAALHIIAEKADGGLRD
                     ALSLFDKIVSFAGKKVSYQDVINNLNILDYDYYFKITDYFISQDSTSTLNAYNEIMQN
                     GFDGEIFIGGLGEHLRNLLVCKAPETVQLLEKNEDLKQRYLDQAQTIDETFLINALSI
                     LNQCDIQYKQAKNKRLHVEIYLLKLCFLGTQIENTLEVLLGEKKN"
     gene            complement(6023..6616)
                     /gene="hisH"
                     /locus_tag="PLS16_08405"
     CDS             complement(6023..6616)
                     /gene="hisH"
                     /locus_tag="PLS16_08405"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNJ61141.1"
                     /translation="MKIVIIKYNAGNTNSVENALQRLAIEPIITDDKEVILSADKVIF
                     PGVGHAAPTMKFLKERNLDQVIKNLKQPTLGICLGQQLMCEHSEEGNVDGLGIFPIQV
                     KKFENSAEKKVPQIGWNSISNLKTNLFENIFEQSFVYFVHSYYCALSSEYTIAQTDYI
                     LNFSAALQKDNFYAVQFHPEKSAAIGHQILKNFIEKI"
     gene            6811..7635
                     /locus_tag="PLS16_08410"
     CDS             6811..7635
                     /locus_tag="PLS16_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNJ61142.1"
                     /translation="MTYTEEQIQSFNVQNFVYLIVEEKENVLTITLNRPEKRNAFHLF
                     LANELAYALAYAHYNNSIWCVVLKANGPTFCAGADLKAFAGENTAEKISTIPMPNDIV
                     KLGDEFNGLHKPCIAQVHADVYAGGFLMIGGCTHVIAVESAKFGLPEVKRGIFPFQVM
                     ATLEPLMSARQLLDLCIRAKILSAKEAQEIGIVSEVVKEENLASRVQELIAEIKEQSP
                     TAIRLGLKAFQEMKTKSAEDKHKYLHAMLMDCLKSKDAAEGLLAFKEKRKPEWKGE"
     gene            7669..8535
                     /locus_tag="PLS16_08415"
     CDS             7669..8535
                     /locus_tag="PLS16_08415"
                     /inference="COORDINATES: protein motif:HMM:NF017652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HNJ61143.1"
                     /translation="MANKKLILSAALTGAATNRAHCPAIPYTPKELGEEAKRAVDAGA
                     SIVHIHAREDNGIPSWRTEVFEEIKAEVRKHSPDVIINFSTGAIGLSVQERIKHLPVT
                     KPDMAAFNMGSMNYAIFSKKQKQFYWNAVFENSFDTMIKVVKTMNENNICPEMECFDT
                     GHIHNVEPLREMGLIPDNACYSLVMGVLGGIPATTENLIHQIKQVPEGAFWQVIGISR
                     KQWQLAATACTMGGNFRVGLEDNFYLPNGEMAKSNGECVEWGVKLARMLDREIASIAE
                     TREMLNLKTINQ"
     gene            8592..10097
                     /locus_tag="PLS16_08420"
     CDS             8592..10097
                     /locus_tag="PLS16_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNJ61144.1"
                     /translation="MTALEQYYQTEEHQLFRKTLRDFLDSEIMPHIDKWEDAGFVPRE
                     IYKKFGDMGFFGISQETEYGGSNLDFWYDVVFIEEVAKCWSGGFSASVSAHPYLALSH
                     MKYEASKSLKDKYLAKAIAGEIVGCLAITEPHAGSDVAGIKTTAMKHGDKYIINGSKC
                     FITNGYLADFMIVACKTGEGASGISMILVEGNEKGVSRNNLNKLGWRASDTAEIAFDN
                     VEVPITNLLGKENKGFYYIMQRFELERLTMSLGAISSSELMMQYTLQYMSERKAFGKS
                     INKFQVLRHKIAQLSAELESVKAYTYLVCQMHSEKKYCVKEASMSKLLATELSDKLAY
                     QCLQMFGGYGYMEEYKAARFFRDSRLGTIGGGTSEIMCEIIAKMVIDDKSYQSTDQNT
                     PQKSITIDELFTTLPDRLKREKAESVQMNNLFEFENDLFYSVIIKDGNLTVQKEKTLN
                     TYDLILNTNTETYIAIETGKQNPQEAFMSGKVKVSDLGKMMQFGSLFKKYS"
     gene            10138..10521
                     /locus_tag="PLS16_08425"
     CDS             10138..10521
                     /locus_tag="PLS16_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNJ61145.1"
                     /translation="MTENDLAYKIIGLAVEVHNALGPGLLESAYQECLYYKINKEGLE
                     VQKEKAIPLIYEAVKLDCGYRIDLLVENKLVIEIKSVETLNDIHLAQTLTYLKLGNYK
                     LGLLMNFNTLLLKSGIKRIINGNLS"
BASE COUNT         3798 a         1470 c         1771 g         3634 t
ORIGIN      
        1 ccttatcatt tagaacagac ttaaatcgtc aatttaataa aactaaaatt agagacatag
       61 gtgatgatgg tttagtatta gacccaactt attttaaata ttttacttgg gatagatttt
      121 ggagtattcg atataattta acacgttcca ttagcgtgga ttatacagca aaccaacaag
      181 ctagaattga tgaacctttt ggagagttga atactaaagc caaacgtgat tcaatgtggc
      241 acaatttctt aaaattagga cgtccaacta attataatca taattttaat gcaagttata
      301 atttgccatt acaacatatt ccatttttgg attggatgac tgttcgtaca agatatggta
      361 gcacttatca atggactgca ccatctttgg cattgcaaag cttaggagca attgctcaaa
      421 actctatgac ttatcaaatt aatgctgatg ttaattttaa aaatctatat aataaagcta
      481 aattcttaaa accatataat acaaatcagc caaagaaaac taaagaagaa tatacaaaag
      541 ctgttgaaca atatgaaaaa caaaatcaga atttcataga taagatggtt caaaaggaaa
      601 aggaaattga gaaaaaaatt gaggatattg aggctgcaaa attagataca accattacaa
      661 gagaacaaat tgaacagatg gttgtggaga aaaaggaatt gaaaaaccaa ttaagaagtt
      721 taaagtattc taaaaaaggt ttaacacaac ctgccaatcc aaaactagat tttgttgttc
      781 gtccattgat gatgttgcag cgtgcatctt taagtttaga cagaacatat actacagttt
      841 tgccaggctt aatgccaaca ccattgttat tcggacaaaa ttttgaacat aaagcaccag
      901 ggtttaaatt tttaatgggc ggacaaagag atactacttg gctaggaaga aaaggagagc
      961 aaggttggat ttcggctgat acaacattaa attaccaatt tatacaaact aaacaaaaaa
     1021 cattcaattt aagagtctct ttagaacctt tccgagattt acgaatagat attaccatgc
     1081 aaaaaacaca aggcgaaaac tattctgaat tttttaagaa aacgggaaat ggtatggcgt
     1141 accaacattt aacgccacaa gtttcaggaa attattctat ttcattttta atgtttagaa
     1201 ctatttttgg taaagttgac gataataatt tcacagcagc ttttagaaat tttgaaaaaa
     1261 acagagaatt atattctcaa aaatttggag ataaaaatcc aaactcaaca ggtatttata
     1321 ttaatgattc tattgtttta ccaaattaca aagagggata tggtccattc tcacaagatg
     1381 ttttgttacc atcattgatt gctgcatata caggtaaaag tattgataga gtaaaattaa
     1441 atccattaaa aactatgcct ttaccaaact ggcgtttaac gtataatgga attacaaaaa
     1501 caaaatgggg taaaaaaatg tttacttcgt ttaatataac acatggctat aattctactt
     1561 ttagtatagg ttcttacatt accaatttga atttcttaaa tacaccaggt tatgtaaatg
     1621 aagatatgta ctacacgcca tataatatgg ataccttaac tggaaattat tattcattat
     1681 atgtagttcc acaagtggct attcaggaac aattatcgcc tttgattgga cttgaagcga
     1741 cttggaaaaa ctctttaatt acaaactttg aatttaagaa atcaagaatg ttgagtttga
     1801 gtatgttgga ttatagatta agtgaaacaa gaacgactga aattacagct gctttaggtt
     1861 ataaaattgc acattttaaa gtgccattta aaattaaagg taaaaaaata acattgaata
     1921 acgatatcaa tttgagagct gatttttcta tcagaaatga taagaccgtc aattatcgtt
     1981 tagatcaaaa tattgctgaa ccagtgcgtg gacaaaaaac aatgtcttta gctgcaacta
     2041 ttgattatat tgtaaatgaa aaattcaata ttagagtttt ctatgacttt agaaaaatat
     2101 tacccgcaac attatcttct taccctacta aaacacatcg tggtggtgta acatttaggt
     2161 tctctttagc tcaataattt atttgtaaat tatgaaggtt tgacaataaa aatatcttat
     2221 cttagcaaaa aattatatta tgcaatttcc atcagattta aagtattcaa aagaacacga
     2281 gtgggtaaaa gttgaaggcg atacagctac aattgggatt acagaattcg ctcaaaaaga
     2341 attgggtgat atcatttatg ttgatattga aactttagga gaagtaatag agcaaaatgg
     2401 aatttttggt tctgttgaag ctgtaaaaac tgtttctgat ttatttatgc ctattgcagg
     2461 tgaaattata gagataaatg cattgttagc agaccaacca gaattagtaa attctgcacc
     2521 ttatggcgat ggttggatgg ttaaaattaa aattacagat gcatctcaag ttgaaagcct
     2581 tttagatagc gaagcatatc aggcgttgtt aggctaaatc tatgttcgat aaaataacaa
     2641 aaactattcc ggcagcaatt tggactgcta tgattttgta tctgtgtttt ctccctggtg
     2701 aaaaaatacc aaaggacgaa tttttgcaaa agatattttt tgacaaattc gtccattttg
     2761 ttttttatta tacacttttc aagttcttac aatttgctgg ttggatagat aagaaatcat
     2821 caattataat ttgtgttgtt cttggattgt ttgtcgaaat cattcaacat ttttatatcc
     2881 ccaaccgtca tttttcatta ggagatattg cagtcaatat tctaggttgt ggtttggctt
     2941 attattttac ttcagaaaag aaagaggagg agccttctaa taaaacaggt atttatttta
     3001 aaaatttaga tgcattgaga gcaattggtt gttggtacgt tacttttgta cacatgttgg
     3061 gcgtatattc tgttaaaaca tctgccaatt tacctgttca aaaaggaagt ttagtggaac
     3121 aatttggaca aggattcttg acagtatttt ttgttttaag cggtttcctt attagttact
     3181 ttttaatcaa agagaaaaca aaaactaata aaattgcatt taagaaattt tatattcgta
     3241 gagcatttag aattttacct atctatttag ttggcttgtt tttagctttt ggtttttata
     3301 aattagtttg tgcaaaagga gctccaggtt tagaaaatgg tatggattac tgtaactcaa
     3361 tcgataaaat atttttattg tatttattta tgattccgaa tgtagcttat tcaattggag
     3421 cattttttcc tcatatttca aattattggt cagttggcgt tgaagaacaa ttttatatag
     3481 catggccgat tgttttacac aaaacaaaaa acattttaaa atcatttgca gttatttata
     3541 ttggatattt tattgtttta cttattactt tggttttata ccaattttat aagaccgata
     3601 attattataa gttctttgat tttgtacatc atgcaagatt cggagctttt gctggaggtg
     3661 gttttttagc atacatcatt ataaatttag aagcatataa aagcaaatat ccaaaagttt
     3721 atttgttttt aatttctaaa aaaactcaaa ttgcttcaat tttattattg ttgttggctg
     3781 cctatatcaa taatatttat ttctatgcat ttaaacatgt ctttattatc attccttgtg
     3841 tttttattat cttgtttaat atgataaacg atgataataa tattttcaaa attgataata
     3901 aattcctcaa ctatgttggg cttacaactt atagtatgta tatcttaaat caaatatttt
     3961 tagatgtttt tgtaggtatg ggaattggta tttttggtgc aaagaatttt atcatggttc
     4021 cattattttc aatgggtatg ctcactttag tttcaatagc atcttataaa tatatcgaat
     4081 taacttttat gaaaataaga ggaaagtatt ttgcttaaaa ggcttctaaa tcaatactaa
     4141 gcttttcgat taaatagaca attttatcat ttttgtctat taattctttt aatttatcaa
     4201 caggtttctt tttaggggca ttatcatctt gctcattttt ctgtacaata atatccaatt
     4261 caattctgtt attattcgta ttactaataa aaaaactgac gatatctgag caatatattt
     4321 gaattatttc ataattgata ttattagaaa cgtaaagaga aatggtattg cctttgattt
     4381 caggaacttg attattaatg atatttataa atgaattttg tgctttaggt ctgatatgct
     4441 caataaaaag cttccaaagt tcaatggcat tttcttgtga aaattcatat ttcttttgtg
     4501 cttctattgt ctcttctttt aaatctttta gcaaatttcc aaatgaagat ttctgagcta
     4561 atattttttt aggctttgta ccttgttgta cacttgtgtt gttagaaatt gtagtggtac
     4621 tattttgtaa tgtaggttga attatatttg atgtttgttc attagattta atattattta
     4681 gattagtttc taatttatga acttctttaa gtttttcaga tattatttgt tgaggaacag
     4741 aaactttttt actttctctt cggattttat attgttcaag aagtgaaatt agtttttttt
     4801 ttcgcctagc aaaacctcta aagtgttttc gatttgtgta cctaagaaac aaagttttaa
     4861 aaggtatatt tcaacatgta aacgtttatt ttttgcttgt ttgtattgta tatcacattg
     4921 atttaaaata ctcaaagcat taatcaaaaa agtttcgtct atcgtttgtg cttgatctaa
     4981 atacctttgt tttaaatctt catttttctc taacaactga acggtctctg gtgctttaca
     5041 aactaataaa tttctaaggt gttctcctaa tccacctatg aaaatttcgc catcaaaacc
     5101 attttgcatg atttcattat aggcatttaa agtagatgta ctatcttgac tgataaaata
     5161 atctgtaatt ttgaaataat aatcataatc caaaatattc aagttattaa tgacatcttg
     5221 ataactaact ttcttaccag caaaacttac tattttatca aaaagcgata aggcatctct
     5281 caaaccaccg tcggcttttt ctgcaataat atgcaatgca gcatcttctg tttcaatttg
     5341 ttcgctatct gcaatatatt gtaattgctt aacaatatca tgattagcaa ttcgcttaaa
     5401 atcaaaaatc tgacatcttg ataaaattgt cggaataatt ttatgcttct ctgttgttgc
     5461 taaaataaaa atagcataac ttggaggctc ttccaatgtt tttaaaaaag cattgaatgc
     5521 acctggagat aacatgtgaa cctcatcaat aatatagact ttatattttc cagcttgtgg
     5581 agcaaatcta acttgatcta ctagcactct gatatcatca acactattat ttgaagcagc
     5641 atctaattca aatatattga aagacgcatt ttgagtaaat gacttgcaag aattacattc
     5701 attacagggt tcaaattctt ctgatggatt ttcgcagttg atagctttgg ctaaaattct
     5761 agcacaggtt gttttaccaa cacctctcgg gccacaaaat aaaaatgatt gagccagttt
     5821 gccactagat aatgcttttt ttaaagtttc agaaacttgt tcttgaccaa caacgtctgt
     5881 aaaacgcatc ggtctgtatt ttcgagccga aactataaaa ttatccatta ctacaaaaat
     5941 aagaaatttt cagcctaata aaaattctaa agctatttaa ttttgaacaa acttctttag
     6001 tgttggaaag tgatttttaa aactatattt tttctataaa attctttaaa atttgatgcc
     6061 caatagctgc tgatttttca gggtgaaact gaaccgcata aaaattgtct ttttgcaaag
     6121 ctgccgaaaa atttagaata taatctgttt gagcaattgt atattctgat gataatgcac
     6181 aataatagga gtgaacaaaa taaacaaaac tttgctcaaa aatattttca aatagatttg
     6241 tctttaagtt tgaaatacta ttccaaccta tttgtggcac ttttttttca gcagaattct
     6301 caaacttttt tacttgaata ggaaaaatgc ctaatccatc aacattgcct tcttcactat
     6361 gttcacacat caattgttgt cctaaacaaa tacctaaagt tggttgcttt aaatttttaa
     6421 tgacttggtc taaatttcgc tcttttaaaa atttcatggt tggtgcggca tgtccaacgc
     6481 ctggaaaaat tactttatct gctgataaaa ttacttcttt atcatcagta ataattggct
     6541 caatagctaa tctttgcaaa gcattttcaa cactattggt gtttccagca ttgtatttta
     6601 tgatgacaat tttcattaat tgaaagtact ttttttctaa atttatacgt gttaatttaa
     6661 aattatagtt gcattaatag aaaatcattg aataattaac ttagcttatg taaaaaagaa
     6721 taccaaacgt ttgtttgatt aagatatttt attactttag ttgaacgaga atgaatttta
     6781 tataaaaatt aatagatagc aaataaaaaa atgacttata ccgaagaaca aatccaaagt
     6841 tttaatgtac aaaactttgt ttaccttatt gtagaagaaa aagaaaatgt actaaccatt
     6901 acactcaatc gaccagaaaa gagaaatgcc tttcatctat ttttagccaa cgaactcgct
     6961 tatgctttag cctatgcaca ttataacaat tctatttggt gcgttgtatt aaaagctaat
     7021 ggccctacat tttgtgctgg tgctgattta aaagcatttg ctggagaaaa tactgcagaa
     7081 aaaatatcta cgattccaat gccaaatgat attgtaaaac taggcgatga atttaatggt
     7141 ttacacaagc cttgtattgc acaagtacat gccgatgtat atgcaggtgg ttttttaatg
     7201 attggtggtt gcacacatgt tattgcggtg gagtcagcaa aatttggctt acctgaagta
     7261 aaaagaggga tatttccttt tcaagtaatg gcaactttag agcctttaat gtctgccaga
     7321 caacttttag atttatgtat aagagctaag attttgtctg caaaagaagc acaagaaatt
     7381 ggaattgtat ctgaagtggt gaaagaagaa aatttagctt cgagagtaca agaattaatt
     7441 gcagaaatta aagagcaatc gccaactgct atacgcttag gtttaaaagc atttcaagaa
     7501 atgaaaacaa aatctgctga ggataaacat aaatacttac atgcaatgtt gatggactgc
     7561 ctaaaaagta aagatgctgc tgaaggcctt ttggcattca aagaaaaaag aaaacctgaa
     7621 tggaaaggcg aataaattat taaaaacacg aagtaataaa ataatatcat ggcaaacaaa
     7681 aaactaatat taagtgcggc acttacagga gctgctacta acagagcaca ttgtccagca
     7741 attccttaca caccgaaaga attaggcgaa gaagccaaac gtgctgtaga tgcaggtgca
     7801 agtatcgttc atattcatgc aagggaagat aacggtattc caagttggag aactgaagta
     7861 tttgaagaaa taaaagcaga agttcgcaaa cattctcctg atgtaattat caatttttct
     7921 actggggcca ttggactttc agttcaagag agaataaagc atttacctgt tacaaaacca
     7981 gatatggcag cattcaatat gggaagtatg aactatgcca tattttcaaa aaaacaaaaa
     8041 caattttatt ggaatgctgt ttttgagaac tccttcgata cgatgattaa agtggtaaaa
     8101 acaatgaatg aaaataatat ttgtccagaa atggaatgtt ttgacacagg tcatattcac
     8161 aatgtagaac ctcttcgaga aatgggctta attcctgata atgcttgtta tagtttggta
     8221 atgggtgtgc ttggaggaat tccagctact actgaaaatt tgattcatca aatcaaacaa
     8281 gtgccagaag gtgcgttttg gcaagtgatt gggataagta gaaaacaatg gcagttggct
     8341 gcaacagctt gtactatggg cggtaatttt cgtgttggat tagaagataa tttttattta
     8401 cctaatggtg agatggcgaa atctaatggc gaatgtgttg aatggggtgt aaaactggca
     8461 cgtatgttag acagagaaat tgcaagtatt gcagaaacta gagaaatgtt gaatttgaaa
     8521 acgataaacc aatagtacaa ttaaagctat aaaactttgt gtttttagta ttagtaagaa
     8581 aatataaaaa aatgactgca ttagaacaat attaccaaac ggaagaacat caactattcc
     8641 gcaaaacact tagagatttt ttagattcgg agataatgcc tcatattgac aaatgggaag
     8701 acgcaggttt tgttcctaga gaaatatata aaaaatttgg cgatatgggt ttttttggca
     8761 taagccaaga aacagaatat ggtggttcta atttagactt ttggtatgat gttgtattta
     8821 ttgaagaagt tgccaaatgt tggagtggcg gttttagtgc tagcgtttct gcacatccct
     8881 atttagcatt atcccacatg aaatatgaag catcaaaatc cttaaaagat aaatatttag
     8941 ctaaagcaat tgctggtgaa attgtaggct gtttagctat cacagaacct cacgcaggaa
     9001 gcgatgttgc aggaattaaa acgaccgcaa tgaagcatgg cgataaatat atcatcaatg
     9061 gaagtaaatg ttttataacg aatggctatt tagctgattt tatgattgtc gcttgtaaaa
     9121 ctggcgaagg agcaagtggt atttctatga tattggtaga aggaaatgaa aaaggtgtaa
     9181 gtcgaaataa tttgaataag ttaggttggc gtgcaagtga taccgcagaa attgctttcg
     9241 ataacgtgga agttcctatc acaaatttat taggcaaaga gaataaagga ttttattata
     9301 tcatgcaacg gtttgaatta gaaagattaa ccatgtcatt aggtgcgatt agttcttccg
     9361 aattaatgat gcaatatact ttacaatata tgagtgaacg taaggctttc ggaaaatcta
     9421 ttaataaatt tcaagtatta cgtcataaaa ttgcacaact cagtgcagaa ttagaaagtg
     9481 tgaaagcata cacttattta gtttgtcaga tgcatagtga gaaaaaatat tgtgttaaag
     9541 aggcttcaat gtctaaacta ttggcgactg aactttctga taaattagca tatcaatgct
     9601 tacaaatgtt tggaggctat ggttatatgg aagaatataa agcagctcga tttttcagag
     9661 attctagatt agggactatc ggtggcggaa catctgaaat catgtgcgag attattgcaa
     9721 aaatggtaat tgatgataaa agttatcaat ccactgatca aaatactcct caaaaaagca
     9781 ttactattga tgaattgttt acgactttac ctgatagatt gaagcgagaa aaggctgaaa
     9841 gtgtacaaat gaataatctt tttgaatttg aaaatgattt attttattcg gttatcataa
     9901 aagatgggaa tttgactgtt caaaaagaaa aaacattgaa tacttacgac ttaatactga
     9961 atacgaacac agaaacttat attgctattg aaactggaaa acaaaaccca caagaagcat
    10021 ttatgtcagg aaaggttaaa gtatccgact taggcaaaat gatgcagttt gggtctttat
    10081 ttaaaaaata ttcttaacca caaagtacac aaagcaaata cacaaagtac acaaagaatg
    10141 actgaaaacg atttagcata taaaataatt ggattagctg tggaagttca caatgcactt
    10201 ggccctggct tattagaaag tgcatatcaa gaatgtttat attataaaat aaacaaagaa
    10261 ggtttagagg ttcaaaaaga aaaagcaata ccattaattt atgaagcagt aaaattagat
    10321 tgtggttata gaatagattt attagtggaa aacaaattag tgattgaaat taagagtgta
    10381 gaaacactaa atgatattca cttagcacaa acactcactt atttaaaatt aggaaattac
    10441 aaacttggtt tattaatgaa ctttaatacg cttttattaa aaagtggcat aaaacgaatc
    10501 ataaatggaa atttatctta gtgtactttg tgaaaaatct tagtgcactt tgtggtaaaa
    10561 aaataaaatt atttaattaa aaatatgtca gcattatctt actatttcaa tgaagagcat
    10621 gaggcttttc gtcagaccat caaacagttt ctaaaaacag aagcagaacc aca
//
LOCUS       DAOQCW010000088        10656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_2320_length_10656_cov_10.488539, whole genome shotgun
            sequence.
ACCESSION   DAOQCW010000088 DAOQCW010000000
VERSION     DAOQCW010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10656
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2320_length_10656_cov_10.488539"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..1066)
                     /locus_tag="PLS16_08430"
     CDS             complement(167..1066)
                     /locus_tag="PLS16_08430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HNJ61146.1"
                     /translation="MKILIAGATGFVGTYLIDYLLEKGYSINALSRSKQVSHHKNLVY
                     FRWDIHQQYIETNAFENVDTIINLTGANIGEKRWTKERKSEIISSRIKSIDLLYQYVS
                     ENKFSINTFISSSAVGYYGAVTTDEMFKETVENGTDFLATVCKEWENTAMKFNDIGIR
                     TVILRKGVIVGKDGGIVQKLAPLAKLGINVSLGSGKQFLPWIDIRDLVKLYGFILSNS
                     KINGIFNAVSKENITMNEFSKTLLKSYKMRNILPNAPSFIIRLLFGEMSVMFLEGSKI
                     SNEKLRSIGFSFEFDTIEKSLLL"
     gene            complement(1063..2367)
                     /locus_tag="PLS16_08435"
     CDS             complement(1063..2367)
                     /locus_tag="PLS16_08435"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNJ61147.1"
                     /translation="MKSSVSIFWFRRDLRLEDNVGLCQALTSGYPILPIFIFDETILS
                     HIENKKDRRVDYIHQALSKINVELKTHQSTLFTFYGKPLDIFKILLEKYDIQGVFCNR
                     DYEPKAIKRDTEIYQFFKTQNIPFKAFKDQVIFDKNEILKNDGTPYTVYTPYSQKWKE
                     KLTVENYQTYKADFTSFFKINFSEIHSLQEIGFQKTEFEFIEPTLNPDIIDEYDKFRD
                     CPAMQRTTQLGIALRFGTISIRKCVAFALEHNQTWLNELIWREFFMQILYHFPKVVTE
                     SFKPKYDFIEWRNNEQEFKLWCEGKTGYPIVDAGMRQLNETGYMHNRVRMIVASFLCK
                     HLLIDWRWGEAYFAQKLSDYDLSANNGNWQWAAGSGCDAAPYFRVFNPALQTEKFDKN
                     LEYIKKWVSEFGTDKYPKPIVEHCFARDRAVKTYGKALKERR"
     gene            complement(2357..2920)
                     /locus_tag="PLS16_08440"
                     /pseudo
     CDS             complement(2357..2920)
                     /locus_tag="PLS16_08440"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
     gene            complement(2917..3606)
                     /locus_tag="PLS16_08445"
     CDS             complement(2917..3606)
                     /locus_tag="PLS16_08445"
                     /inference="COORDINATES: protein motif:HMM:NF039186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HNJ61148.1"
                     /translation="MMSFTYILVLFFTVIICFIASFDKRLQFNKHFGAFLKASVLVAI
                     PFIAWDVWFTAKGVWWFNINYTMGLNIAGLPLEEWLFFIFIPFSCIFTYYSIDKFFKM
                     EWLSGFNNLIVFITVIVCSVIALLYYNKIYTIITSLSAILTVVYLHFIAKADWISKAS
                     LVFTILMLGFLPVNGILTGTGLDTPIVNYNPDDFLGIRILTIPIEDSVYGYTQFLLVL
                     YFFKHFKQSKK"
     gene            complement(3603..4061)
                     /locus_tag="PLS16_08450"
     CDS             complement(3603..4061)
                     /locus_tag="PLS16_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNJ61149.1"
                     /translation="MNILITILVFVLMEGATWLIHKYIMHGFLWVLHKDHHDHSNKGV
                     LEKNDYFFVIFALPTIALMYFGTLEDFNYLFFIGLGIMLYGMAYFFVHDIFIHQRIKY
                     FTHTKNPYFKALRRAHKQHHKHLGKEEGECFGFLYVPFKYFRMYFKSNKK"
     gene            complement(4065..4523)
                     /locus_tag="PLS16_08455"
     CDS             complement(4065..4523)
                     /locus_tag="PLS16_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNJ61150.1"
                     /translation="MKHQLYREQQLNCDIEIAWEFFSSPYNLAQITPKEMGFVVHTDV
                     GNQAIFEGMEIEYTVSPLLKIPLKWRTKITEVTFQKSFTDFQEKGPYKLWNHFHEFIP
                     NQEGVLMKDTVDYELPFGFLGDITHALLVKKKLESIFDFRFKVLEQKFNK"
     gene            complement(4526..4981)
                     /locus_tag="PLS16_08460"
     CDS             complement(4526..4981)
                     /locus_tag="PLS16_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61151.1"
                     /translation="MKWTIFLLSALFIFSSFAIYSLDDVRANYSQLATDKKMCSELLA
                     QLEKNKDQSATHLAYLGGLQTIWANHVLNPLSKLNTFNKGKKNIELAIEQEPNNAELR
                     FIRLSLQKNAPTFLEYHSNIKEDIQFIKNNRSQIKSNVINQYIESLLKN"
     gene            complement(4981..5784)
                     /locus_tag="PLS16_08465"
     CDS             complement(4981..5784)
                     /locus_tag="PLS16_08465"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HNJ61152.1"
                     /translation="MSKKTTKKYSTSFSLGILALKPSIRPAIYAIYGYVRLADEIVDS
                     FQGYDKEKLLNRLKLETDNALSEGISLNPILQSFQDTVVKYNIDRTLINQFLHSMEMD
                     LQKIDYNSALYEEYIHGSAEVVGLMCLQVFTEGDKSKYEELKPYAMKLGSVFQKINFL
                     RDLKDDYQILGRTYFPNVDMCLFDNCVKCQIEEDIEQEFKQALIGIKMLPNSSKFGVY
                     LAYKYYLTLFKKIKNKSSKEIMNNRVRVANSEKAYVAFKSYLRYKTTCL"
     gene            complement(5821..7287)
                     /locus_tag="PLS16_08470"
     CDS             complement(5821..7287)
                     /locus_tag="PLS16_08470"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HNJ61153.1"
                     /translation="MKIAIIGSGFSGLSAASYLAKDGNEVHVFEKHDQAGGRARQFCT
                     PQGYVFDMGPSWYWMPDIIERFFADFGYKQTDFFELVSLNPQFEMIFSDLKMDVPESL
                     DDLKSLFEKIEPSAGKQLEKFMQSAKFKYEVGMQDFVNKPCHNWSEFISPKIAKSVLK
                     FGLLTNFRSYVARYFKNEKLRTLMEFPVIFLGASPKNIPALYSLMNYGGYALGTHYPM
                     GGFYQLVLAMKSVAEKQGAKFHFNKTVEYINTQNGEVKSLTINGENIEFDAVIASSDY
                     HHTESLLDSEFRNYDEQYWKKRTFAPSSLIFYLGINEAIPNLKHHTLFFENELDDHID
                     CIYGHKRWPEKPLFYACCPSKTDDNVAPKGKENLFLLMPVAIGINDDESIREKYLRDM
                     LLRIEKHTGISDLKSKIEYQRSYCVRDFVSDYNAYGGNAYGLANTLNQTAVLKPKLRN
                     KKLKNLYYTGQLTVPGPGVPPSIISGKIVANEVNRLNK"
     gene            complement(7284..7934)
                     /locus_tag="PLS16_08475"
     CDS             complement(7284..7934)
                     /locus_tag="PLS16_08475"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNJ61154.1"
                     /translation="MNYGLLKDIIDLVEQYELENSKTNEITVNGFGRWIACNLEENGV
                     EPDWEGKENGRSPESVINTLIVHMNRYAKSYSKSAIIDSDFSTQEDFIFLINLKAFGE
                     MTKMELIKKNVQEKPAGMQIINRLIAQKWVAQKDSKTDKRSKVISITPKGLKALEDQM
                     DKIRQATHIVTGNLSRSEKMELIRLLNKLNDFHLPIYEKNIESENLLAEVLKMTRT"
     gene            8045..10471
                     /locus_tag="PLS16_08480"
     CDS             8045..10471
                     /locus_tag="PLS16_08480"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ61155.1"
                     /translation="MKRLIFILVATMLSVQLNAQTLGTVSGTVKDKNTQEELIGVAIQ
                     FFGSDTLGVVTDENGSFSIKIPVGKYNLKASYIGYEVFEQYNLNVSSGNALLLQIELD
                     SKSHALEEVVINAGKSVRATDMVTPLATQKLTAEEIKSNPGGNFDVSKVIQVLPGVAG
                     GTTPNRNDIIVRGGGPSENVYYLDGIEIPVLNHFQTQGASGGATGILNVSFIQDVQLT
                     SSAFNARYDNALASTIVVKQRNGNPDKLSGNFRLSGTEFAAMLEGPLGKKTTFMASAR
                     RSYLQFLFKLLDLPIRPDYYDFQYKVNHKINNKTELNFIGIGAIDNFKLAAPKKSDAN
                     TEYINRANPLIKQWNYTVGATLKRLVNNGFFTIALSRNMFFNGADRYENNASKSGNKL
                     FSLGSHEIENKLRFDMNMFKNGWKYSYGVDAQYVKYDASIFNTVKDEIKDSLGNTISP
                     AISFSTNSAIDFFKFGAYGQVAKYFFDNKFLVSAGIRTDINTYTKNGLNPLRTLSPRA
                     SFSYILNNKWNISASVGSYYKLPVYTMLGYKDNNGTLSNKDLKYTNSIHYTIGTEFVP
                     KNDLRVTFEAFYKDYRNYPISLSNGISYANIGTDYSAVGSDKYTSTGRGRVYGIEAYI
                     QQKLIKNLFYVASATLYKSEFSGIDGNFKPSTWNYGFIVSATFGYKFKKNWDIGLKYR
                     IAGGQPYTPFDMNASTAMYLTTGTGVYDYTQLNNKRLPIFNQLDLRVDKKFNFKKVSL
                     DIFLDFQNILFYKTPYLPKFTFERTDDNTGFKTTDGQPIKSDGSNGIPLILNQRSATI
                     VPSIGFIFEF"
BASE COUNT         3583 a         1859 c         1568 g         3646 t
ORIGIN      
        1 ttataggcaa gcgtagaaca accgaaccct caagattatt acagaaatta gtctgagatt
       61 tgttttattt ttttgccaac gctcgttttg cacttttggt tttgcagaaa tacgaaacca
      121 tcactaaaaa aaccaaaaga gctaaatttt tcctttcact gcaaaatcac aataaaagag
      181 acttttctat agtatcaaat tcaaaagaaa agccaatact cctcagcttt tcattactga
      241 tttttgatcc ttctaaaaac ataaccgaca tttcgccaaa aagtaaacgg atgataaatg
      301 aaggtgcatt gggtaggata tttctcattt tataagattt caacaaggtt tttgaaaatt
      361 cattcatcgt gatattttct tttgaaacag cattaaaaat tccgttaatt ttagaattag
      421 aaaggataaa tccatacagt tttactaaat ctctaatatc aatccaaggt aaaaactgtt
      481 ttcctgaacc caacgaaaca tttattccta atttagctaa cggtgctaat ttttgaacta
      541 ttcctccatc ttttccaact atcactcctt tgcgtaaaat aactgttcta atacctatat
      601 cgttaaattt cattgctgta ttttcccatt ctttgcaaac agtggctaaa aaatcagttc
      661 cattttcaac agtttcttta aacatttcat ctgtagttac tgctccataa tacccaacgg
      721 cagaagacga aataaatgta ttgatgctaa atttattttc cgatacatac tgatacaata
      781 agtcaataga cttaatacga ctgctgatga tttcactttt ccgttctttt gtccatcttt
      841 tctctccaat gttagcacca gttaaattga taattgtatc tacattttca aaggcgtttg
      901 tttcaatata ttgctgatga atatcccatc gaaaatacac taaattctta tgatgactaa
      961 cttgtttact ccttgataag gcattaatgg aatatccttt ctctaataaa taatcaatta
     1021 gatatgttcc aacaaagcct gttgcacctg caataagtat tttcatcttc tttcttttaa
     1081 tgcttttcca taagttttca ctgctctatc tcttgcaaag caatgttcta caataggttt
     1141 gggatattta tcagttccaa attctgacac ccattttttg atatattcta agtttttatc
     1201 aaatttttca gtttgcaatg ctggattgaa aactcggaaa tatggagcag catcgcaacc
     1261 cgaacctgct gcccattgcc agtttccgtt attggcagat aaatcataat cgctgagttt
     1321 ttgagcaaaa taagcttctc cccaccgcca gtcgataagt aaatgtttac ataaaaaact
     1381 ggcaacaatc atccgcactc tattgtgcat ataaccggtt tcgttgagct gtcgcattcc
     1441 tgcatctaca attggatagc cggtttttcc ttcgcaccaa agcttaaatt cttgttcatt
     1501 atttctccac tctataaaat catatttggg tttgaatgat tcagttacaa cttttggaaa
     1561 atgatataga atttgcataa aaaattcacg ccatattaat tcgtttaacc aagtttgatt
     1621 gtgttctaaa gcaaaagcaa cacatttgcg aatactaatc gttccaaagc gaagtgcaat
     1681 gcccaattgt gtggtgcgct gcatagcagg acaatctcga aatttgtcat attcatcaat
     1741 aatatcagga ttaagtgtag gttcaataaa ttcaaattct gttttttgaa aacctatttc
     1801 ttgtaatgaa tgaatttcgg aaaaatttat tttaaaaaaa ctagtaaaat cagctttata
     1861 tgtttgataa ttttctaccg ttaatttttc tttccatttt tgggaatacg gagtgtaaac
     1921 agtgtaaggc gttccatcat ttttaagtat ttcgttttta tcaaaaataa cttggtcttt
     1981 aaatgctttg aatggaatat tttgcgtttt gaaaaattga taaatttcgg tatctctttt
     2041 tatcgctttc ggttcataat ctcgattgca gaagacacct tgaatatcat atttctccaa
     2101 aaggatttta aaaatatcca acggtttccc ataaaatgtg aacaaagttg attgatgcgt
     2161 ttttagttct acatttattt tagaaagtgc ctgatgaata taatctactc ttctatcttt
     2221 cttgttttct atatgtgata aaatagtttc atcaaaaata aaaatcggca aaataggata
     2281 gcccgaagtc aatgcttgac ataatcctac attatcttcc aaacgcaaat cacgcctaaa
     2341 ccaaaatatg gaaacactac ttttcattca ggaaaggatt gtcttcttta tcgtgtttta
     2401 tccaaattaa tgttgaagta tcatagccaa tttcttcagc aatttttaag tattccgttt
     2461 tgatttcttc aggtaattct ttagttcgtg acaatatcca caaataatcc aagtttttac
     2521 ctgcaattaa agcatattga taattctcgt ccaatgcaat cacattgtat cccgaataaa
     2581 acggtccaaa gaagctaact tttagagcag caatattctt atcgcctcag aactttgcta
     2641 atccatcggc tttactccat ttatttttta caaaattata cccactgttc aacacaatta
     2701 catttccctt tttatctaaa gtataatttg ccgaagtgtt gtctaaatct ttttcaaaac
     2761 ggaaatcaaa acgagccact tcataccaag tacctaagta acggttcacc tcaaaattct
     2821 ctacaggttt tgccttttga ggaatagaag cacaagagtt aaagagtatg ataattccaa
     2881 tcactactat taaagaaatt attttacttt gttttttcat ttttttgatt gtttaaaatg
     2941 tttaaaaaaa tatagaacta agagaaattg cgtataacca taaaccgaat cttctatagg
     3001 aattgtcaat attcgtatgc ctaaaaaatc atcaggattg taattcacaa taggcgtgtc
     3061 taaacctgtt ccagtaagaa ttccatttac aggaagaaac cctaacatta aaattgtaaa
     3121 gactaaagat gctttgctaa tccaatctgc ttttgctata aaatgaagat aaactactgt
     3181 aagaatagct gaaagcgatg tgattattgt gtaaatctta ttataatata ataatgctat
     3241 taccgaacag acaataacag ttataaaaac gattaaatta ttaaatccac tcaaccattc
     3301 cattttaaaa aacttgtcta tactgtaata agtaaaaatg cacgaaaaag gaatgaagat
     3361 aaaaaacaac cattcttcca aaggcaagcc ggctatattt aatcccattg tataattgat
     3421 attaaaccac catacgcctt ttgcagtaaa ccaaacatcc caagctatga aaggaatagc
     3481 tactagaaca gatgctttta gaaatgctcc aaaatgcttg ttgaactgta atcgcttgtc
     3541 aaaagatgca ataaaacaaa tgataactgt gaaaaacaag acgagtatgt aggtgaaaga
     3601 catcattttt tattgctttt aaaatacatt ctgaaatatt tgaaaggcac atacaaaaat
     3661 ccaaaacatt cgccttcttc ttttcctaag tgcttgtgat gttgtttatg agctcttctt
     3721 aaagctttaa aataagggtt tttagtgtgg gtaaaatatt taattctttg gtggatgaag
     3781 atatcgtgca caaaaaaata cgccattcca tatagcataa tgcccaagcc aataaagaac
     3841 aagtaattaa aatcttcgag tgtgccaaag tacatcaaag caatcgttgg caaggcaaag
     3901 atgacaaaga aataatcatt tttttctaaa acgcctttat tactatggtc gtgatggtct
     3961 ttatgcaata cccaaagaaa accgtgcatt atgtatttat gaatgagcca agtagctcct
     4021 tccattagta caaaaacgag tatggttatt aatatattca tatattattt attaaatttt
     4081 tgctccaaaa ctttaaagcg aaaatcaaaa attgattcca gcttcttttt aaccaataaa
     4141 gcgtgagtga tatctcccaa aaagccgaaa ggtaattcat aatctaccgt atctttcatt
     4201 aacacgccct cttgatttgg aataaattcg tgaaagtgat tccagagttt ataaggtcct
     4261 ttttcttgaa aatcggtaaa gcttttttga aaagtaactt ccgtaatttt cgttctccat
     4321 ttcaacggta ttttaagtaa aggagaaacg gtatattcaa tttccatacc ttcaaaaatc
     4381 gcttggtttc ctacatcggt atgcactaca aatcccattt ctttaggagt aatttgggca
     4441 aggttatagg gcgatgaaaa aaactcccaa gcgatttcta tatcacaatt aagttgttgt
     4501 tctctatata actgatgttt cattattaat tttttagtaa cgattctatg tattgattga
     4561 ttacatttga tttgatctga cttcgattgt ttttaataaa ttgtatatct tctttaatat
     4621 tggaatgata ctctaaaaaa gtgggtgcat ttttttggag cgacagccgt ataaatcgta
     4681 attcagcatt attgggctct tgttcaatag ctagttcaat attttttttc cctttattaa
     4741 aagtattgag tttgcttaat ggatttaaaa cgtgatttgc ccaaatggtt tgcaaacctc
     4801 ctaaatatgc taaatgggta gccgattggt ctttattctt ttccaattga gccagtaatt
     4861 cactacacat tttcttatcg gtagccaatt ggctataatt tgcccttaca tcatctaagg
     4921 aataaatggc gaaactgcta aaaataaaca gagctgataa aagaaaaata gtccatttca
     4981 ttacaaacaa gttgttttat acctgagata actcttaaat gcaacatacg ctttttcaga
     5041 attagcaaca cgtacacggt tattcataat ctctttagaa gatttatttt tgattttttt
     5101 aaacaaagtc aaataatatt tgtaagccaa atacacgcca aatttcgaag aattaggaag
     5161 cattttaatt ccgattaatg cctgtttaaa ttcttgttct atatcttctt caatttggca
     5221 tttcacacaa ttatcgaaca aacacatatc aacattcgga aaataagtac gaccaaggat
     5281 ttgataatca tctttcaaat ctcttaaaaa attaattttt tgaaaaaccg aacctaattt
     5341 cattgcataa ggcttcaatt cttcgtattt agatttatct ccctcggtaa aaacttgcaa
     5401 acacattaaa ccaaccactt ccgctgaacc gtgaatgtat tcttcataca atgcagaatt
     5461 ataatctatt ttttgcaaat ccatttccat actgtgtaga aattgattta ttaaagttct
     5521 gtctatattg tattttacaa cggtatcttg aaaagattgt aaaattggat tcaatgaaat
     5581 gccttcggac aaagcattgt cggtttccaa ttttaatcta tttaataatt tttctttatc
     5641 ataaccttga aagctatcca caatttcatc agctaatctt acataaccat aaatagcata
     5701 aatagcaggt ctaattgacg gctttaacgc tagtatgccc aacgaaaaac tggtgctata
     5761 cttttttgtt gtctttttac tcacatcgta agacaattct tcaaacagtt ttttaatttt
     5821 ttatttattt aatcggttaa cttcatttgc tactattttt cctgatatta ttgatggcgg
     5881 aactccaggt cccggtacgg ttaattgtcc agtgtagtat aagtttttta attttttgtt
     5941 cctgagtttt ggttttaata ccgctgtttg atttagcgta ttagccaaac cataagcatt
     6001 ccctccgtaa gcattgtaat cagaaacaaa atcacgcaca caataacttc tttggtattc
     6061 aattttagat tttaaatcac tgatgccggt gtgtttttcg attcttaaca gcatatctct
     6121 aagatatttt tcacggatac tttcatcatc attaattcct atcgctacag gcatcagtaa
     6181 aaacaaattt tcttttcctt ttggggcaac gttatcatct gtctttgaag gacagcacgc
     6241 ataaaacaaa ggtttttcag gccatctttt atgaccgtaa atacaatcaa tatggtcatc
     6301 taactcgttt tcaaagaaaa gcgtatgatg ttttaaatta ggaatcgctt cattaatacc
     6361 caaataaaaa atcaaacttg atggagcaaa agttcttttc ttccaatatt gctcatcata
     6421 atttctgaac tcgctatcca aaagtgattc agtgtggtga taatccgaag aagcaataac
     6481 tgcatcaaat tcaatattct ctccattaat tgtaagagat tttacttccc cattttgagt
     6541 attgatatat tcaacagttt tattaaaatg aaatttagcc ccttgttttt cagccacact
     6601 tttcattgct aaaaccaact ggtaaaaacc gcccattgga taatgtgtac ccagagcgta
     6661 acctccgtag ttcatcagac tatacaatgc aggaatattt tttggagatg ctcctaaaaa
     6721 tataacagga aactccatca aagttctcag tttttcattt ttaaaatatc gggcaacata
     6781 acttctgaaa tttgtcagta aaccaaattt caaaacactt ttagcgattt tgggtgatat
     6841 aaattcactc caattatgac aaggtttgtt tacaaaatct tgcattccaa cttcatactt
     6901 aaactttgcc gattgcataa atttctccaa ctgtttgcct gcactaggtt ctattttttc
     6961 aaaaagactt ttcaaatcat ccaaactttc aggaacatcc atttttaaat ccgaaaaaat
     7021 catttcaaac tgtgggttta aagaaaccaa ttcaaaaaaa tccgtttgtt tgtacccaaa
     7081 atccgcaaaa aatctttcaa taatatcagg catccaatac cagcttggtc ccatatcaaa
     7141 aacatatcct tggggcgtgc aaaattgcct tgcccttccg cctgcttggt catgcttttc
     7201 aaaaacgtgt acttcatttc catcttttgc caaataggaa gcggccgaca aacccgaaaa
     7261 tcctgaacct ataatggcta ttttcatgtt ctagtcattt ttaatacttc cgctaacaaa
     7321 ttttctgatt ctatattttt ctcataaata ggtaaatgga aatcgttcaa cttattaagg
     7381 agtcttatca actccatttt ttccgaacga cttaaatttc ccgttacgat atgtgtggct
     7441 tgcctaattt tatccatctg atcttccaaa gcttttaaac ctttgggtgt aatagaaatt
     7501 accttacttc ttttgtcggt tttagaatct ttctgtgcga cccacttttg agcaattaat
     7561 cgattaatga tttgcatacc tgcgggtttc tcctgcacgt tttttttgat taattccatc
     7621 ttagtcattt caccaaatgc ttttagattg attaaaaaaa taaaatcttc ttgtgttgaa
     7681 aagtcagaat caattattgc tgatttggaa taactcttag catagcgatt catgtgtact
     7741 ataagagtgt taatgacact ttcaggactt cttccattct cttttccttc ccaatcaggc
     7801 tctactccgt tttcttccaa attacaagca atccatcttc caaaaccgtt gactgtaatt
     7861 tcattcgttt ttgaattttc caactcatat tgttctacta aatcaataat atcttttaat
     7921 aaaccgtaat tcattactaa aaattctagg atacaaagat actactattt ttaaataaag
     7981 tacaatatta cactattatt taaaaataaa atttactttt gcactaaata aatagattta
     8041 aagtatgaaa aggttgattt tcattcttgt tgcaacaatg ttatctgtac aactcaatgc
     8101 tcaaacatta gggacggttt caggtactgt gaaggacaag aatacacaag aagaattaat
     8161 tggtgtagct atacaatttt ttggttcaga tactttaggt gttgtaactg atgaaaatgg
     8221 ttctttttct atcaaaatac cagtaggaaa atataattta aaagcttctt acattggata
     8281 tgaagtattt gaacaataca atctaaatgt aagttctgga aatgctcttt tacttcaaat
     8341 tgaattagat tcaaaaagtc atgcattaga agaagttgtt attaatgcag ggaaatctgt
     8401 tagagccact gatatggtaa ctccgctggc gactcaaaaa ttaactgccg aagaaataaa
     8461 atctaatcca ggtgggaatt ttgatgtttc aaaagtgatt caagttttac cgggagttgc
     8521 aggcggaacc acacccaaca gaaacgatat tattgttagg ggtggtggac ccagtgaaaa
     8581 tgtatattac ttagacggga ttgaaattcc tgttttaaac cattttcaaa cgcaaggtgc
     8641 gagtggtgga gcaacaggta ttttaaatgt ttcgtttata caagatgtgc aacttacttc
     8701 atcggctttt aatgccagat atgataatgc tttggcttca acaattgttg tcaaacaacg
     8761 aaatggaaat cctgataaat taagtggaaa ttttcgcttg tcaggaacag aatttgcagc
     8821 aatgttagaa ggtcctttag gaaaaaaaac tacttttatg gcttcggcaa gacgctcgta
     8881 tttacaattt ttatttaaat tattagattt gccgattaga cctgattact acgattttca
     8941 atacaaagta aaccacaaga taaacaacaa aacagaacta aattttattg gaattggtgc
     9001 tattgacaat tttaaattag ctgctcccaa aaagagtgat gcaaatacag aatacatcaa
     9061 ccgtgcaaat cctttaatta aacagtggaa ttatacagtt ggagcgacat taaaaagatt
     9121 agtaaacaac ggattcttta ccattgcgtt aagtaggaat atgtttttta atggtgctga
     9181 ccgttatgaa aataacgcct ctaaaagtgg taataaattg ttcagtttgg gaagtcacga
     9241 aatagaaaac aaacttcgtt ttgatatgaa tatgtttaaa aatggttgga aatacagtta
     9301 cggagttgat gctcaatatg taaagtacga tgcttctatt ttcaacacag ttaaggatga
     9361 aataaaagat agtttaggaa atacgattag ccctgctata tctttttcaa ccaattcagc
     9421 aattgatttt tttaaatttg gtgcgtatgg tcaggttgct aaatacttct ttgataataa
     9481 atttttagta tcggctggta ttcgaacaga catcaatacc tatacaaaaa atgggttaaa
     9541 tccattaaga acactatctc caagagcttc attttcttat atcctcaaca ataaatggaa
     9601 tatttcggca tcggtgggga gttattacaa attacctgtt tacacaatgc ttggatataa
     9661 agacaacaac ggaactctta gcaataaaga cttaaaatat accaattcca ttcattacac
     9721 aatcggaact gaatttgttc caaaaaatga tttacgggtt acttttgaag cattttataa
     9781 agattacagg aactatccaa tatcgttaag taatgggatt tcttatgcta atataggcac
     9841 ggattattcg gcagttggca gcgataaata cacttcaaca ggacgaggaa gagtttatgg
     9901 aatcgaagca tatattcaac aaaaattgat taaaaatcta ttttacgtag ccagtgccac
     9961 gctttacaaa tctgaatttt caggtataga tggaaatttt aaaccttcaa cttggaatta
    10021 tggatttatt gtttctgcaa cttttgggta taaattcaaa aagaattggg atataggctt
    10081 aaaatacaga attgcaggag gacaacctta tacgcctttt gatatgaacg catctacagc
    10141 tatgtatctt accactggaa cgggtgttta cgattatacg caactgaaca acaaacgact
    10201 tcctattttc aaccaattgg atttgagagt agataaaaag ttcaatttca aaaaagtaag
    10261 cttagatata tttttagatt ttcaaaatat tttgttctac aaaacacctt atttaccgaa
    10321 atttactttt gaaagaaccg atgacaatac aggttttaaa acaacggatg gacaacctat
    10381 taaatcagac ggcagtaacg gtattccttt aattttaaac caaaggtctg ctacaattgt
    10441 tccttcaatt ggatttatat ttgagtttta atgaatagca aaataccaga catacacatt
    10501 ggcaagctcc acaagccaat actcaaagtt aagcctgcca aagagtatgt cttaatggag
    10561 aaaaataaaa caaattatac gatttaaaga aagccagcct gtaacattaa gcggattgaa
    10621 aaagcccgac cacatacgcg aggtacctta aaaaac
//
LOCUS       DAOQCW010000089        10624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_2333_length_10624_cov_5.041726, whole genome shotgun sequence.
ACCESSION   DAOQCW010000089 DAOQCW010000000
VERSION     DAOQCW010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10624
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2333_length_10624_cov_5.041726"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2268)
                     /locus_tag="PLS16_08485"
     CDS             complement(<1..2268)
                     /locus_tag="PLS16_08485"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNJ61156.1"
                     /translation="MSTKAQIISFTPPPEDDQPSVQDIIKRIEDTLNIRQGKVKMEDT
                     RLAEDKLKQIAPTPNTIGVDKNAPDKLLNEAQKATETVEKLTETATLELRENLDTVDI
                     FGFDFFRRKEVKLFNNAVDIKPSLNYVLGVGDQIVVSIWGYADYNRLFYIDKDGYIQQ
                     ENIGRVYLKGLTLEQAKELLKTRFSNAYMIDKSNFDVSINYSRVITINVVGDVENPGS
                     YTFPAINTAYNILTYVGGPSKSGSLRDIQIKRDGKIYQKFDLYKFMFEPEKQGDIFLN
                     NNDYIYVPLQKNIVKISGEINKPGKYELLPGETLTNLLGFCGGFKPNAFKTVVQVKRY
                     LENSTVILDFNLDSLKALGKQLELFNGDEVLIRTIPEPLKNFVEIKGSVMLPGKYELV
                     DGFKVKDIILKAQGFKDDIFMDEAYLVRTNIDDGRKNIFKINLFKVMNEPNSPDNMVL
                     QIRDEIETYSKAYFLDTFSISITGEVRMPRKFSLEKEMTLRDAVMMSGGFTDFAFLDK
                     IIIYRVNENLADEMLSFKIDTTNNYAALDSVKLQKRDRIIVKKDLSKLDKYNVEVNGM
                     VRKPGVFSYHENMTLADAIILADGFKMEAASNRIEIARVANFDKAIAQSVPTQMSILS
                     FSIGKNIGKDELANSTLLQPYDQIFVRPTPEFEFQTKVSVVGEVMYPGDYVLVSKDEK
                     LSSLIDRAGGLTRFAYPKGARLFRKTDGIGYVLMDLDKAVKRKNSKFNYILNKGDSIV
                     IPPVKDLVVLQGAFNY"
     gene            complement(2324..3379)
                     /gene="rfbB"
                     /locus_tag="PLS16_08490"
     CDS             complement(2324..3379)
                     /gene="rfbB"
                     /locus_tag="PLS16_08490"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNJ61157.1"
                     /translation="MKKSILITGGAGFIGSHVVRLFVNKYPDYQILNLDNLTYAGNLE
                     NLKDIENKPNYKFIKADIVDANKMNEIFTNFPIDSVIHLAAESHVDRSIANPLEFIMT
                     NVVGTVNLLNAAKSAWKDNFEGKLFYHVSTDEVYGSLGETGLFLETTPYDPRSPYSAS
                     KASSDQFVRAYENTYGLPIVISNCSNNYGANQFPEKLIPLFIHNIKNKKPLPVYGDGK
                     YTRDWLWVEDHASAIDLIFHKGKLGETYNIGGFNEWQNIDLIKVLCKLMDEKLNQSQG
                     TSEQLITYIKDRPGHDKRYAIDATKLNQELGWSPSLQFEEGLSKTIDWYLSNEEWLSN
                     VTSGNYQNYYKTQYETI"
     gene            complement(3379..4677)
                     /locus_tag="PLS16_08495"
     CDS             complement(3379..4677)
                     /locus_tag="PLS16_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNJ61158.1"
                     /translation="MSEVFNRLKNKETKLAVIGLGYVGLPIALELANKFSVVGFDINE
                     KRVDLMKQKIDPSNELPQSAFDNKDILFSSNIEDLKLATFYIVAVPTPVDQHRTPDLK
                     PLIGASTTVGKVIKKGDYVVYESTVYPGCTEDDCVPIIEKLSGLKAVEDFKVGYSPER
                     INPGDTEHTLTKILKIVSGCDAESLEVIAEVYESIIEPGVYRASSIKVAEAAKVIENT
                     QRDLNIALMNELSQVFSKMNINTYEVLEAAGTKWNFLRFQPGLVGGHCIGVDPYYLTY
                     KSMALGYEPKIILSGRNINDGMPQYIAKKIVKNWLKIGKNPTECRVLILGATFKENVT
                     DIRNSKIFDLYKELVDYNAKVDIIDPNADAEEVLHEYEVQLNTQPAGKYDAIIVSVAH
                     DEYKLLNEDYFVSLSNSHCVLLDLKNIYKGTFKKLNYWTL"
     gene            4839..5942
                     /locus_tag="PLS16_08500"
     CDS             4839..5942
                     /locus_tag="PLS16_08500"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HNJ61159.1"
                     /translation="MSILAIFKPKIALWFHLQKDWKLKLNKTDLKDVIWFHVASVGEF
                     EMAIPIIKQIKSTQRLQKVVVSFFSTSGINVYTSDENVDYFFLYPKDYKKNINFIIKN
                     IQPKCLILVKYERWLNMVQQTYLKKIPIYLVEDEQKIYSFFYQKYVNYFQQFIKKTFY
                     IKNGSLQIEKVKTTKKINLPIIQEFTSNHFTIILGSCYIKEVKLISSFYNKYSNLKII
                     LCPHEITEHTFNQYQAFFNEKISKYSDSIIESNILFIDKMGILKHIYPYTKIAFIGGG
                     FNNKLHNIFEAAIYDNIILTGNQINRNFLNTLADEAQLIYKIESLNNLEFIITRAINS
                     PDFINDNKEKWCNFFKLHQNTAQFITDTILSDL"
     gene            complement(5937..7364)
                     /locus_tag="PLS16_08505"
     CDS             complement(5937..7364)
                     /locus_tag="PLS16_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61160.1"
                     /translation="MEIRSSLKKIDNYLKIVSDLNKAILDEGTMAPEELQIMKKYLYV
                     CADRIEDIQIKFGLATTETENGILDNLKQNEEAEVTLTEFAEIESENQRSEEYDILKE
                     LEPSDENSLNVSDSIVDKDDDVINTVNEAQIVQDEQKVSEQNIKSDERILVEPVVFLS
                     ADLPSIRYNTLIDFDDYHQQIFDEKPEAKSVMNGIGEIQNNSNTLVADDVEKVEEKIE
                     FKANVEIKEPIELVAETKEETQNDDNLINTLVADDVEKVEEKIEFKANDETKEPIELV
                     AETKEETQNDDDSINTLVADDVEKVEEKIEFKTDNEVKNPIQLTSENSELNPSISTNI
                     EEKTILVLDEVENDKILVDFEKKPETSEEREHFNLVDTANEQKDVNVFTKTTSSRTEY
                     ESIVNRKSLSEQIALNDKFIFVRELFASQFSEYESSIKEIDVFENYQDALDFCTNVLS
                     VKYNWEARKNISERFLNILYKKYNL"
     gene            7416..7982
                     /locus_tag="PLS16_08510"
     CDS             7416..7982
                     /locus_tag="PLS16_08510"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNJ61161.1"
                     /translation="MFLEQNVNNSKNGWIEVVCGSMFSGKTEELLRRIKRANIANLKT
                     EIFKPMIDTRYDETAVVSHDANSIQSTPVSSSQNILLLSHQVDVVGIDEAQFFDDGLP
                     EVCSQLANKGIRVIVAGLDKDFLGKPFGCMPQLMAIAEYVTKVHAICVQCGNLANFSY
                     RLISNEKIVLLGEKESYEPRCRTCFNQQ"
     gene            7992..8504
                     /locus_tag="PLS16_08515"
     CDS             7992..8504
                     /locus_tag="PLS16_08515"
                     /inference="COORDINATES: protein motif:HMM:NF027363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HNJ61162.1"
                     /translation="MKIANFEIPDEICINNKKLCLNGAAMRSKFFINTYIIALYCEKP
                     IQNEEDAIHSSIPRCLRMIITTPLATPSAVSQNIGNGLKDSMSSSDYKKIQPLLENIK
                     EIIEKTNTGYKDTIDNFYDSDKTFYYYKNDSLLGKTDVEGQILAEAIFDLYLGKKPKD
                     PKIKKTLLGK"
     gene            8520..8936
                     /gene="aroQ"
                     /locus_tag="PLS16_08520"
     CDS             8520..8936
                     /gene="aroQ"
                     /locus_tag="PLS16_08520"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNJ61163.1"
                     /translation="MKKIIIINGPNLNLLGKREPEIYGSQTFQDIFNQLQIDNPTIEL
                     SYYQSNIEGELINKIHEIGFNFDGIVLNAGAYTHTSIAIADAIKSISTPVIEVHISNT
                     FAREPFRHHSYISPVAKGIIVGFGFKSYQLALDSLK"
     gene            8943..9344
                     /locus_tag="PLS16_08525"
     CDS             8943..9344
                     /locus_tag="PLS16_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HNJ61164.1"
                     /translation="MIDLNEIILFDGICNFCQGSVKFIIKYDKTKRLKFASLQSEIGQ
                     KLRTTYHIPEDTDSIVFIKNKKAYVKSSAALRIATYLSFPLNWLYIFIIIPTFIRNFF
                     YDIIAKNRYKWFGKQDACMLPTPEIRSRFLD"
     gene            9430..9909
                     /locus_tag="PLS16_08530"
     CDS             9430..9909
                     /locus_tag="PLS16_08530"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HNJ61165.1"
                     /translation="MMLKSFNIELIADIRSLPGSRKYPQFDKENLEIELPKNNILYKH
                     FPNLGGRRKVNKDSPNTSWRNDAFRGYADYMETDAFENAIKELEQIALKQRTCYMCAE
                     AVWWQCHRSMVSDYLKAKKWKVYHIMNIGKAQEHCYTQPARIIGDKVVYTESNLFEP"
     gene            complement(9926..10597)
                     /locus_tag="PLS16_08535"
     CDS             complement(9926..10597)
                     /locus_tag="PLS16_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNJ61166.1"
                     /translation="MKNKIAIVSGANGNLGRAVVQYFLAQKWLVIGLEHQLSNLIKEE
                     NFQEIAVNLLDEKATADCVQNIIANYGRIDVLVHTVGGFAMGNIADTSTQDIFKQYQL
                     NFETTYNLVRPILDKMQNQKNGDLFFIGSLAGKDTSKGKGVTAYSLSKSLLFQLAKLI
                     EAESIGTAIQTHVVVPSIIDTPQNRAAMPNADFSKWEKPELIAKVIGQYFGKEKQVII
                     IQDEM"
BASE COUNT         3636 a         1740 c         1326 g         3922 t
ORIGIN      
        1 ataattaaat gctccttgca acactactaa atccttaaca ggtggaataa caatagagtc
       61 tcctttattt aaaatataat taaattttga attcttacgt tttacagctt tatctaaatc
      121 cattaaaaca tatccaatcc catcagtttt tctaaataat ctagctcctt tgggataagc
      181 aaatctagtt aaaccacctg ctctatcaat aagagatgag agcttttcat ctttagaaac
      241 taacacataa tctcctggat acatcacttc acctacgaca gaaacctttg tttgaaattc
      301 aaattcaggt gttggcctaa caaaaatctg atcgtatggt tgaagtagtg tcgaatttgc
      361 cagttcatct ttcccaatat ttttaccaat actaaaactc aatatagaca tctgggtggg
      421 aactgattgt gcgatagctt tatcaaaatt agcaactctt gcaatttcaa tacgattaga
      481 agctgcttcc attttaaatc catcagccaa gatgattgca tcagctaagg tcatattttc
      541 atgataagaa aacactccag gttttcttac cattccattg acttcaacat tatatttatc
      601 taatttggat aaatcttttt ttacaataat tctatctctc ttttgtaatt taacagaatc
      661 taaagctgca taattattcg ttgtatcaat tttaaatgat agcatttcat ctgctaaatt
      721 ctcattgacg cgataaatta taatcttgtc taaaaaagca aaatcagtaa aaccaccact
      781 catcattact gcatctctca acgtcatttc tttttcgagc gaaaattttc taggcattct
      841 tacttcgcca gttattgaaa tactaaaagt atctaagaaa tatgctttag agtaggtttc
      901 tatttcatct ctaatttgaa gaaccatatt atcaggcgaa ttcggttcat tcattacttt
      961 aaataaatta attttaaata tattttttct accatcatca atatttgttc taaccaaata
     1021 tgcttcatcc ataaaaatat catctttgaa cccttgagct tttaaaataa tatcttttac
     1081 tttaaaacca tctactaatt cgtatttccc tggcaacatt acagaacctt tgatttcaac
     1141 aaagtttttt aatggttctg gtattgttcg gattaatact tcatcgccat taaataactc
     1201 taattgttta cctagtgctt tcaagctatc taaattaaaa tctaaaatta ctgttgaatt
     1261 ttctaaatat ctttttactt gaacaactgt tttgaatgca ttaggtttga agccaccaca
     1321 aaatcctaat aaattagtta aagtttcgcc tggtaacaac tcatatttcc ctggtttatt
     1381 tatttcaccg ctaattttaa caatattttt ttggagcgga acatatatat aatcattgtt
     1441 atttaagaaa atatctcctt gtttttcagg ctcaaacatg aacttataaa ggtcaaattt
     1501 ctgataaatt ttaccgtctc ttttgatttg aatatctcta agtgaacctg attttgaagg
     1561 accacctaca taagttaaaa tattatatgc cgtattgatt gctggaaatg tataagaacc
     1621 aggattttca acatcaccaa ctacattaat tgtaatcact ctagaataat taatagatac
     1681 atcaaaatta gatttatcta tcatataagc atttgagaaa cgtgttttta ataactcctt
     1741 tgcttgttcc aaagtcaaac cttttaaata tactcttcct atattttctt gttggatata
     1801 accatcttta tcaatataaa ataatcggtt gtaatcagca tatccccaaa tcgaaacaac
     1861 aatttgatca ccaactccta aaacatagtt taatgaaggt tttatatcta ccgcattatt
     1921 aaaaagtttt acttcttttc ttctaaaaaa atcaaaacca aaaatatcta cagtatctaa
     1981 gttttctctt aattctaaag tagcggtttc tgttaacttc tcaactgtct cagtcgcttt
     2041 ttgtgcttca tttaaaagtt tatctggtgc atttttatca acaccaatcg tattcggtgt
     2101 aggtgctatt tgttttagtt tatcttctgc aaggcgagta tcttccattt ttactttacc
     2161 ttgtctgatg tttagtgtat cttctatacg tttaataata tcttgaacac ttggttgatc
     2221 atcttcaggt ggtggtgtaa atgaaataat ttgagctttt gttgacaaat ttaaaatgaa
     2281 aacaagtaca gtaaaaagat atattttttt caaaaaatcc atattatatt gtttcgtatt
     2341 gggttttata atagttttga tagttccctg aagttacatt acttaaccat tcttcatttg
     2401 acaaatacca atctattgtt ttactcaaac cctcttcaaa ttgtaaggag ggcgaccaac
     2461 ctaattcttg attaagtttt gtagcatcaa tagcatatct tttatcgtgt cctggtctat
     2521 ctttgatata ggttattaat tgttctgaag taccttggga ttgatttagt ttttcatcca
     2581 ttaatttaca aagtactttt atcaaatcaa tattttgcca ttcattaaat cctccaatat
     2641 tataagtttc tcccaatttg cctttgtgaa aaatcaaatc aattgcacta gcatgatctt
     2701 ctacccaaag ccaatctctt gtatattttc cgtctccata aacgggtaat ggttttttat
     2761 tttttatatt atgtataaaa agtggtatta atttttcagg aaattgatta gcaccataat
     2821 tattggaaca atttgaaatc actattggca aaccgtaagt attttcgtaa gcacgtacaa
     2881 attgatccga acttgctttt gaagcagaat aaggagagcg tgggtcataa ggtgttgttt
     2941 ctaaaaacaa tcctgtttct ccaagagaac catacacctc atcggttgaa acgtggtaaa
     3001 aaagcttacc ttcaaaatta tctttccatg ctgattttgc tgcatttaac aaatttactg
     3061 taccaactac attggtcatg ataaactcta aaggattggc aatagatcta tctacatgtg
     3121 attctgcagc taaatgaata acactatcta ttggaaaatt tgtaaaaatt tcattcattt
     3181 tattagcatc tacaatatct gctttaatga atttataatt aggtttattt tcaatatctt
     3241 ttaaattttc tagatttcct gcatacgtca aattatctaa atttaaaatt tgataatcag
     3301 ggtatttatt tacaaataac ctaacgacat gagaccctat aaatccagct cctcctgtaa
     3361 ttaaaattga ttttttcatt ataaagtcca atagtttaat tttttaaaag ttcctttgta
     3421 aatattcttc aagtccaata atacacaatg tgaattggat aaacttacaa aataatcttc
     3481 atttaataat ttatattcat catgagctac agaaactata atcgcatcat actttccagc
     3541 aggttgagta tttaattgaa cctcatattc atgtaatact tcttcagcat cagcatttgg
     3601 gtcaataata tctacttttg cattataatc taccaattct ttatataaat caaatatttt
     3661 tgaatttcta atatctgtaa cattttcctt aaaagtcgca cccaatatta aaactctaca
     3721 ttctgttgga ttttttccaa ttttcaacca atttttaact attttctttg ctatatattg
     3781 tggcatccca tcattaatat ttctaccgct taaaatgatt ttaggttcat aacctaatgc
     3841 catagattta taagttaaat aataaggatc tacacctata caatgtcctc caactaaacc
     3901 tggttgaaat cttaggaaat tccattttgt accagcggct tctaaaactt cgtaagtatt
     3961 gatattcatt ttactaaata cttgagaaag ttcattcatc aaagcaatgt tcaaatcacg
     4021 ttgtgtattt tcaatcactt tagcagcctc tgcaactttt atacttgatg ctctatacac
     4081 gcctggctca ataatagatt cgtaaacctc tgcaataact tctaaagatt cagcatcaca
     4141 accagaaaca attttcaata ttttggtcaa tgtatgttct gtatctcctg gatttatcct
     4201 ttctggagaa taacctactt taaaatcttc aacggctttt aatcctgata atttttcaat
     4261 aattggcaca caatcatctt ctgtacaacc tggataaacc gttgattcat aaacaacata
     4321 atcccctttt ttaataacct ttccgacagt agtagatgct ccaattaatg gtttcaaatc
     4381 tggagttcta tgttgatcta ctggtgttgg gacagcaaca atataaaaag ttgctaattt
     4441 taaatcttca atattggaag aaaataaaat atctttatta tcaaaagcag actgtggcaa
     4501 ttcattactt ggatctatct tttgtttcat taaatctacc cgcttctcat tgatatcaaa
     4561 accaacgaca gaaaatttat tagctaattc taatgcaata ggtaatccca cataacctaa
     4621 gccaattaca gcaagtttgg tttctttatt ttttaatcgg ttaaatactt cgctcatttt
     4681 ttattaatct gtacaaatat aaaaatattt agacttatat tttatactta atttcgttaa
     4741 ttagtattat tctatctatt tttattgcgt tttataatct aattagactt ttgattacca
     4801 aaatatacca gttaataata catctcattt atttcacaat gagtatcctt gctattttca
     4861 aacccaaaat agcactttgg tttcatttac aaaaggactg gaaattaaaa ttaaataaaa
     4921 cagatttaaa agatgttatt tggtttcatg ttgcttctgt tggcgaattt gaaatggcaa
     4981 ttccaatcat caaacaaata aaatcaacac aacgcttaca aaaagtagta gtttcctttt
     5041 tctctacctc tggaataaat gtttatacct ctgatgaaaa tgtagattat tttttccttt
     5101 atcctaaaga ttataaaaag aatataaatt ttattataaa aaatattcaa ccaaaatgtt
     5161 tgattttggt aaaatacgag cgatggctaa atatggttca acaaacatat ttaaaaaaaa
     5221 ttccaattta tctagttgaa gatgaacaaa aaatatattc atttttctat cagaaatacg
     5281 tcaactattt ccaacaattt atcaaaaaaa cattttatat aaaaaatggt tcactgcaaa
     5341 tagaaaaggt aaaaactact aaaaaaatca atcttcctat cattcaagag tttacttcaa
     5401 atcattttac aattatttta ggtagttgct atattaaaga agtaaagctc atctcctcat
     5461 tttataacaa atattcaaac ctaaaaatta ttctttgtcc acacgaaata accgagcata
     5521 cattcaacca atatcaagca ttttttaatg aaaaaatttc taaatattct gactcaatta
     5581 ttgaatctaa tattttgttc atcgataaaa tgggcattct aaaacatatt tatccttata
     5641 caaaaattgc ttttattgga ggtggtttta ataataaatt acacaatatt tttgaggctg
     5701 ccatatatga taatattatc ctaactggca atcaaattaa tagaaatttc ttaaatacat
     5761 tagctgatga agctcaactt atttacaaaa ttgaaagttt aaataattta gaatttataa
     5821 ttactcgggc tataaactct ccagacttta taaatgataa caaagaaaaa tggtgtaact
     5881 ttttcaagtt acaccaaaat acagctcaat ttataactga tactattcta tctgatttat
     5941 aaattatatt tcttatacaa aatatttaga aatctttctg aaatattttt ccttgcttcc
     6001 caattatatt ttacggacaa tacattcgtg caaaaatcta gtgcatcttg ataattttca
     6061 aaaacatcaa tttctttaat agaactttca tattcagaaa attgactagc aaataattca
     6121 cgcacaaaaa taaacttatc atttaaggca atttgttcac ttaatgattt tctatttaca
     6181 atgctttcat attcagttct gcttgaagta gtcttagtaa acacattaac atccttttgt
     6241 tcattagcgg tatccaccaa attaaaatgt tctctttctt cactagtttc aggtttcttt
     6301 tcaaaatcga ctaatatttt gtcgttttca acctcatcta aaactaatat tgttttttct
     6361 tcaatgttcg tagaaatgga tgggtttaat tcagaattct cagaagtcag ttgaattggg
     6421 tttttaactt cgttatctgt tttaaattca attttttctt caactttttc gacatcatct
     6481 gcaactaaag tatttattga atcatcatca ttttgagttt cttcctttgt ttcagctact
     6541 aattcaatag gttcttttgt ttcatcattt gctttaaatt caatcttctc ttcaactttt
     6601 tcaacatcat ctgctactag agtatttatt aaattatcat cattttgagt ttcttccttt
     6661 gtttcagcta ctaattcaat aggttctttt atttcaacat ttgctttaaa ttcaattttc
     6721 tcttcaactt tttcaacatc atcagctact aaagtatttg aattattttg tatctctcca
     6781 attccattca ttacactttt agcttcaggc ttttcatcaa aaatctgttg atggtaatcg
     6841 tcaaaatcta ttaatgtatt atatctaatt gacggcaagt ctgcacttaa aaacacaact
     6901 ggttcaacta aaattctttc atcagattta atattttgct ctgaaacttt ttgctcatct
     6961 tgaacaattt gcgcttcatt aactgtatta attacatcat catctttatc aacaatacta
     7021 tcagaaacat ttagagaatt ttcatcagaa ggttctaatt cttttaaaat atcatattcc
     7081 tcactgcgtt gattttcgga ttcaatttca gcaaattcgg tcaaggtaac ttctgcttct
     7141 tcattttgct ttaaattgtc taaaattcca ttttcagttt ccgttgtagc taagccaaat
     7201 ttaatttgta tatcttcaat acgatcagca catacgtata aatacttctt cattatttgc
     7261 aattcttccg gagccattgt gccttcatct aatattgctt tatttaagtc cgaaacaatt
     7321 tttaaataat tatctatttt tttcaacgaa ctacgtattt ccatgatatt ttgtattttg
     7381 gtgtcaattt ctacaaaaat aaaacaaaat aaattatgtt tttagaacaa aatgtgaata
     7441 actctaaaaa cggatggatt gaagtcgttt gtgggtctat gttttccggc aagactgaag
     7501 agcttttacg tagaataaag cgagcaaata ttgctaatct gaaaactgaa attttcaaac
     7561 caatgataga tactcgttac gacgaaactg ctgttgtttc tcatgacgcc aattcaattc
     7621 aatcgacacc tgttagtagt tctcaaaaca tattattatt aagtcatcaa gttgatgttg
     7681 ttggtattga tgaagctcaa ttttttgatg atggtttacc agaagtttgc tcacaattag
     7741 caaacaaagg tattcgagtg attgttgcag gattagataa agatttttta ggcaaacctt
     7801 ttggctgtat gccacaatta atggcaattg ctgaatatgt taccaaagtt catgcaatat
     7861 gtgttcaatg cggcaatttg gcaaattttt cctatcgctt aataagtaac gaaaaaatag
     7921 tacttttagg tgaaaaagaa agttatgaac caagatgtcg aacttgtttt aatcaacaat
     7981 aatttagaaa aatgaaaatt gctaattttg aaatccctga tgaaatttgt ataaataaca
     8041 agaaactttg tttaaatgga gcagctatgc gatcaaagtt ctttattaac acatatatta
     8101 ttgcattata ttgtgaaaaa cctatccaaa atgaagaaga tgctatccac tcttcaattc
     8161 caagatgttt aagaatgata attacaacac ctttggcaac gccatcggct gttagtcaaa
     8221 acataggcaa tggattaaaa gattctatgt catcgtcgga ttataaaaaa attcaacctt
     8281 tattagaaaa catcaaagaa attatagaaa aaacaaatac tggttataaa gatactattg
     8341 ataatttcta tgatagtgat aaaacatttt attattataa aaacgatagt ttattgggaa
     8401 aaactgatgt agaagggcaa attcttgcag aagcaatatt tgatttatat ttaggaaaaa
     8461 aacccaaaga ccccaaaatt aaaaaaacac ttttaggaaa ataatattta gcttttaata
     8521 tgaaaaaaat catcattata aatggtccaa atctaaattt attaggaaaa cgagaaccag
     8581 aaatctatgg cagtcaaacc tttcaagata tttttaatca attacaaatt gataatccaa
     8641 caatagaatt gagctattat caatctaata tagaaggcga attaattaat aaaatacacg
     8701 aaataggttt taattttgat ggtattgttt taaatgctgg tgcttatacg cacacttcta
     8761 ttgcaattgc agatgctata aaatcaattt caacaccagt tattgaggtg catattagca
     8821 atacttttgc acgggaacca tttagacatc attcttatat ttcgccagtt gccaaaggta
     8881 ttattgttgg ctttggtttt aaaagttatc aactggcttt agatagttta aaataagata
     8941 ttatgattga tttgaatgaa attattctat ttgatggaat ttgcaatttc tgccaaggct
     9001 cagtaaaatt tattataaaa tatgataaaa ctaaaagact aaaatttgct tcattacaat
     9061 ctgaaattgg acaaaaactt aggacaactt atcatattcc agaggacacc gatagtattg
     9121 tttttattaa aaacaaaaaa gcttatgtaa aatcaagtgc tgctttaaga attgcaactt
     9181 acttatcttt tccactaaat tggctgtata tttttataat aataccaacc tttattagaa
     9241 atttttttta cgatatcatt gcaaaaaata gatacaaatg gtttggaaag caagatgctt
     9301 gtatgttacc tacgcctgaa atacgttctc gttttttaga ttaaactatt tcaaaaaaaa
     9361 atgataaagt aatcagaaaa aatcatctac acgataggac attctacaca tagctttaat
     9421 gaatttattt tgatgctaaa gtcttttaat attgaattaa ttgcagatat cagaagtttg
     9481 cctggctcaa gaaagtatcc tcaatttgat aaagaaaatt tagaaattga attgcctaaa
     9541 aataatattt tatataagca ttttccaaat ttaggtggca gaagaaaagt aaacaaagat
     9601 tctcctaata caagttggcg taatgatgca tttcgtggat atgcagatta tatggagaca
     9661 gatgcttttg aaaatgcaat caaagaactt gagcaaattg ctttaaaaca aagaacttgt
     9721 tacatgtgtg ctgaagctgt ctggtggcaa tgtcatcgct ctatggtgtc cgattattta
     9781 aaagctaaaa aatggaaagt ttaccacata atgaatatag gtaaagctca agaacattgt
     9841 tacacgcagc ctgccagaat tattggcgat aaggtggttt ataccgaaag caacctattt
     9901 gaaccataaa aaaatatcga tgaatttaca tttcgtcttg gataataata acttgttttt
     9961 cttttccaaa atattgtcca attactttag caattaactc aggtttttcc cattttgaaa
    10021 aatcagcatt aggcattgcg gcacgatttt gtggtgtatc tattatactt ggaaccacaa
    10081 catgtgtttg tattgcagta cctatcgact ctgcctcaat taactttgct aattgaaata
    10141 aaagtgattt tgataaactg tatgcggtaa cgcctttgcc ttttgatgtg tcttttccag
    10201 ccaaagaacc aataaaaaac aaatcaccat ttttttgatt ttgcattttg tccaaaatcg
    10261 gtcgaactaa attataagtc gtttcaaaat ttaattggta ttgcttgaaa atatcttgag
    10321 ttgaagtatc agcaatattt cccattgcaa accctcctac tgtatgaact aatacatcta
    10381 ttctaccata atttgctatt atattttgaa cacaatctgc ggttgctttt tcatctaaga
    10441 gattgactgc tatttcttga aaattttctt ctttaattaa attactcaat tgatgttcta
    10501 atcctataac caaccatttt tgagctaaaa aatattgcac aactgcacga cctaaatttc
    10561 catttgctcc actaactatc gcaattttat ttttcatata tcaattacta tattttacct
    10621 aaaa
//
LOCUS       DAOQCW010000090        10587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_2343_length_10587_cov_6.005602, whole genome shotgun sequence.
ACCESSION   DAOQCW010000090 DAOQCW010000000
VERSION     DAOQCW010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10587
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2343_length_10587_cov_6.005602"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PLS16_08540"
     CDS             complement(<1..357)
                     /locus_tag="PLS16_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HNJ61167.1"
                     /translation="MASHFESPLREPLVKGNKTYKQITTDIVRSTTGELQNRWIFWTT
                     ISAIVATFGVFCIAWEVVFGIGSWGANKTVGWAWDITNFVWWIGIGHAGTLISAILLL
                     FRQKWRTGVNRAAEAMT"
     gene            complement(402..3437)
                     /locus_tag="PLS16_08545"
     CDS             complement(402..3437)
                     /locus_tag="PLS16_08545"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HNJ61168.1"
                     /translation="MSNKKYWTSLDELNQTPAYFEQANQEFSLVEDATESIENKEPNR
                     RDFLKAMGFSVSVAAVASACQIPVKNAIPYTFDLRNQMPEVVPGVAEFFATTYYGSAD
                     FNSILVKTREGRPIKVEGNPSAKFFGGTSARAQAAVLDLYDVTRLHNPVKANKKIDWK
                     KADAEIIKKLDELKASGQKVVVVTNTILSTITKKAIEQLKTTYANVSVVAYDPISQYA
                     IRKASGGIIPAYQFDKADVIVGFNCDFLGTWMNGTENAAKYVTKRVPTPENPTMSRHH
                     QFQSSMSLTGASADYKYPIKPSEEKQVLINLYTKLGGSVSGAKDFSNKELNTGIDKVA
                     KELVAAKGKSIVVSGTNDVEIQSLVNAINATLSNFGATIDTSNTLNFKQGNDEALATL
                     ASELKNGAYKGIILVGANPVYDTVYAETFKNALQKADLSISTADRVDESAQYCQYVAP
                     NSHLLESWDVIEPRTNVYGFVQPTITALFDTRQESASILTWAGNVNVAIDSLVQAEIA
                     SVSGLSYSEALQAGFVDKTSGIGASALPGISTITTPTQPKGMELFLYEKVAIGDGKLA
                     NNPFLQEMPDPISKATWDNYILVPYSFAKENKIDSMPADKKVPVGKITVAGKSFELPI
                     VVQFGQAQNTLAIALGYGREKSGNVGSGVGANLYPILKNNTNSVDGVTFELTGKTYNL
                     GLNQTYHTLMEDVSLPGKPLRYRGMVVKETNLASYSKSETAGNEDREAIQKHLVTMYP
                     TPKFPGHHWGMSVDMNACIGCGACVVACNIENNIPVVGRRQVFRSQEMHWLRIDRYYN
                     GNPDNPDVSFQPMMCQHCDNAPCENVCPVNATNHSSEGINQMAYNRCIGTRYCANNCP
                     FKVRRFNWLDYQGNDMFGSFNDGTPGWGGSETKDYMFDSLTRMVLNPDVVVRTRGVIE
                     KCSFCTQRIQSSKLDAKKQNRKLQDGDVKTACQTACPTNAIVFGDMNDKNSQVAQTLD
                     SGRNYFLFEEQHFLNHVGYQVKIRNKDEESQRSFFDKEG"
     gene            complement(3469..4785)
                     /locus_tag="PLS16_08550"
     CDS             complement(3469..4785)
                     /locus_tag="PLS16_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNJ61169.1"
                     /translation="MFNSYIKRSYSLLVSFFLIFVISTSSYAGVAVPDVDPADAAAVA
                     KAKTGMDLFKANCTACHAIDRKVIGPALADVWDKWDTEENLIAWVKNSQAVIKSGHAH
                     ANKIYEEYNKMPMPAFPTLSDEQVKDVFLYMKAAEKGFDPSNPGGKKETADAAGGATT
                     EGADSGKSNTTLILVGLFAVLAILGAVLFNITSKLSKIAKVAQGESLDAIEAVEAASK
                     KALVKKLGVVAVIIAAVLLLFNIGNGAINLGRSQGYKPEQPIRFSHALHVGQNKIECQ
                     YCHTGVEKSRHASIPGTQICMNCHKYVQEGPKYGKDEIKKIYDYSGWDPDKGAFVKAP
                     KNIEWVQIHNLPDHVYFNHSQHVKVGKIECQTCHGKIEEMETVEQFAPLSMGWCINCH
                     RETKVQFADNNYYSVFEKYHDEIKKGKRAGVTVEDIGGTECQKCHY"
     gene            5050..5655
                     /gene="rsmG"
                     /locus_tag="PLS16_08555"
     CDS             5050..5655
                     /gene="rsmG"
                     /locus_tag="PLS16_08555"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNJ61170.1"
                     /translation="MDIILKYFKELSPKQIAQFKQLLHLYTEWNSKINVISRKDMENF
                     YERHVLHSLSIADFFDLSHHQIIDIGTGGGFPGIPLAIFFPESQFHLVDSIGKKISVV
                     NAIAEALELKNITAQHGRAEQCTNLFDYAVTRAVAPAADLIHWTSKNVNKKNGIIALK
                     GGDLREELKAVNCKKEIYHLSAVYSEPFFETKKIIHLHQIK"
     gene            complement(5657..6631)
                     /locus_tag="PLS16_08560"
     CDS             complement(5657..6631)
                     /locus_tag="PLS16_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNJ61171.1"
                     /translation="MFLIILNYKKDMSKKLPILILFFAISFSLFAQHYSNIIQGRIMD
                     VDKNIPVIGATLNLRFNSDVYTAKTDSSGNYNITTFVRYLDGKYDINITHPDYYELNG
                     VVLVKEISEHNFGLKHKREVVKTIDTVVQPLSKLEGFASNNWTLLVDMSSSMGDNNIL
                     PVLKSGLNDIVDYFRSEDKITLLTFSSGISEILPPTNGNNKDKIHNAIASLIKVGGTT
                     NGASAIKSAFQKASENYINNGNNRILIFTDGMFTSGAKEYKNIEKILADYAQKNINCS
                     IFLLGKPTDYVIKQLEFLANAGEGTFAVLKDETTAKQKMLDEAKLVRK"
     gene            6656..7399
                     /locus_tag="PLS16_08565"
     CDS             6656..7399
                     /locus_tag="PLS16_08565"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNJ61172.1"
                     /translation="MNKIISFLFLIFFGLNIIAQNDTYISKYKYKWGVDAPIIGITVG
                     TGAAAYFLHKNKKIITVDEINALSPTMVNKFDRSAIFHDNKGAKISSDVLLYGASLAP
                     ALMFIDKAVRKEWKYYLPMWIEVYGLTSGLTLMTKEIVKRKRPYVYNDKLSAESKVGK
                     DATASFFSGHTSTTAASTFFMAKVFADLHPDSKFKPLMWTGAALIPAITGLCRYGAGK
                     HFFTDILVGYAIGSAVGILVPQLHKRKIQ"
     gene            complement(7459..8244)
                     /locus_tag="PLS16_08570"
     CDS             complement(7459..8244)
                     /locus_tag="PLS16_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNJ61173.1"
                     /translation="MQYKVPVMADLAAEGKSPDVLFWIGCAGSFDQRAQRITQAFVKI
                     LNQLNISFAVLGKEEACTGDPARRAGNEFIFQMLAQQNIATLNGYGIKTIVTTCPHCF
                     NTIKNEYPALGGKYEVLHHSVYLQQLLNDGKLKVEGGAFKGKRITYHDSCYLGRANDI
                     YEAPRDLIKALDADLVEVTKSRANAMCCGAGGAQMWKEDEPGTKRINTERTGQLTEVM
                     PDIIAVACSYCNTMITDGVKSENKQDEIKVVDLAELIAENNKF"
     gene            complement(8287..9627)
                     /locus_tag="PLS16_08575"
     CDS             complement(8287..9627)
                     /locus_tag="PLS16_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNJ61174.1"
                     /translation="MFLTIIQMILFYALTGTVGYFAFKKFKEVYDNIKMGQPETIDTP
                     LSFRLKNMFLIAFGQKKMFKITFPALLHLVIYLSFVITQIELIEIMLDGATGQHRILF
                     HYIFQGNILEGFYRFVVSFIEVLSLLTFFVTIVFILRRTVFKINRFNKPEMKGWPTKD
                     GLLILAFELTLITFIFMMNGADRALQLQGDPHYIDTKGFLLSNFLGHSLMSLPHGALV
                     MMERIGWWGHLSMVFAFMLYLPYSKHLHILLAFPNTFFSRPEPKGQIRNMESVMHEVK
                     SMFDDSYQAPETDGEIKFGAKDVFDLSWKSLLDAYTCTECGRCTQACPANQTGKILSP
                     RKIMIDTRDRMEEITRNRKIHGPDFTDNKTLLNDYVTSEELRACTTCNACVEECPVNI
                     SPLNIILELRRNMIMDESNSPQEWNTMFANMENNQAPWQFSMEDRLNWTDDLNK"
     gene            complement(9934..>10587)
                     /locus_tag="PLS16_08580"
     CDS             complement(9934..>10587)
                     /locus_tag="PLS16_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNJ61175.1"
                     /translation="LDEIGRRLALGNFKELNLIIKGDVDGSVEALSDSLLKLSTEEVQ
                     INIIHKGVGQISESDVLLASASDAIIIAFQVRPSANAKLIAEKEDIQIKMYSVIYSAI
                     EEVKSAMEGLLEPKFEEKIVGNVEVREVFKVSKIGTIAGCYVTDGKIVRSNEVRIIRD
                     GIVVFEGGLDSLKRFKDDVKEVTKGYECGIKIKNYNDIREGDIIESFEQVEVKRKLS"
BASE COUNT         3473 a         1909 c         1459 g         3746 t
ORIGIN      
        1 agtcatcgct tctgcagctc tatttacacc tgttctccat ttttgtctaa acaataataa
       61 gattgctgaa attaaagtac cagcatgacc aataccaatc caccaaacga agttggtaat
      121 atcccatgcc caacctactg ttttatttgc accccaagat ccaataccaa atacaacttc
      181 ccatgcaata caaaacacac caaaagtcgc aacaattgca gatattgttg tccaaaatat
      241 ccaacggttt tgtaattcac ctgtcgttga acgtacgata tcagtagtaa tttgcttata
      301 ggtcttatta ccttttacta agggttctct aagtggtgat tcaaaatgac ttgccatatc
      361 taagctttag ttatttatat attctattaa ttattaattt actatccttc tttatcaaag
      421 aaacttcttt gagattcttc atccttattt ctgattttaa cttgatatcc aacatgattt
      481 aagaaatgtt gttcttcaaa taagaaataa tttctacctg aatcaagagt ttgtgctact
      541 tgactatttt tatcattcat atctccaaac acaattgcat ttgtcggaca tgctgtttga
      601 caagctgtct taacatctcc atcttgcaat tttctgtttt gctttttagc atctaattta
      661 ctagattgaa ttctttgtgt acagaaacta catttttcaa taacccctct tgttctcaca
      721 acaacatctg gatttaatac cattcttgtt aaagaatcaa acatataatc tttagtttca
      781 ctaccacccc aacctggcgt tccgtcatta aatgagccaa acatatcatt accttgatag
      841 tctaaccagt taaaacgacg aactttaaat ggacagttat ttgcacaata tcttgtacca
      901 atacatctgt tatatgccat ttggttaata ccttcagaac tatggttagt tgcatttact
      961 ggacaaacgt tttcacaagg tgcattatca caatgctgac acatcatagg ttggaaagat
     1021 acatctggat tatctgggtt tccattatag tatctatcta tacgtaacca atgcatttcc
     1081 tgacttctga aaacttgtct tcttccaaca actggtatat tattttcaat attacaagct
     1141 actacacaag caccacaacc aatacatgca ttcatatcta ccgacattcc ccaatggtgt
     1201 cctgggaatt taggtgttgg atacatagtt actaaatgtt tttgtattgc ctctctatct
     1261 tcattacctg cagtttcact ttttgaatat gaagctaaat tcgtctcttt aacaaccata
     1321 cctctatatc ttaatggctt acctggtaaa gaaacatctt ccattaaggt atgataagtt
     1381 tggtttaaac ctaaattata tgtttttccc gtcaattcaa aagtaacacc atcaactgaa
     1441 tttgtgttat tttttaagat tgggtataaa tttgctccaa caccagaacc tacattacct
     1501 gatttttctc ttccgtaacc taatgcaata gctaaggtat tttgagcttg accaaattgt
     1561 acaacaatcg gtaattcaaa agattttcct gcaacagtaa ttttaccaac tggtactttt
     1621 ttatcagcag gcattgaatc aatcttattt tctttagcaa atgaataagg aactaaaata
     1681 taattatccc aagttgcttt agaaattgga tctggcattt cttgtaagaa aggattgttt
     1741 gccaatttac catcaccaat cgcaactttc tcatataaaa ataactccat accttttggt
     1801 tgcgttggag ttgttattgt actgattcca ggcaaagcac tagctccaat tcctgaagtt
     1861 ttatctacaa aaccagcttg taaggcctca gaataagaca agccacttac tgaagcaatt
     1921 tcagcttgaa ctagagaatc tatagcaaca ttaacatttc ctgcccaagt taagatagaa
     1981 gcactttctt gtcttgtatc aaataaagct gtaattgttg gttgcacaaa accataaacg
     2041 tttgttctag gttcaataac atcccaagat tctaataaat gtgaatttgg agcaacatat
     2101 tgacaatatt gagcagattc atctactcta tcagctgtag aaatacttaa atctgctttt
     2161 tgtaaagcat ttttaaaagt ttcagcataa actgtatcat aaactggatt agcaccaact
     2221 aagataatac ctttatatgc accatttttt aattctgatg ctaaagttgc taatgcttca
     2281 tcattacctt gcttgaaatt taaagtattt gaagtatcaa tagttgcacc aaagttagat
     2341 aaagtagcat tgattgcatt cactaaagat tgaatttcaa catcatttgt acctgaaact
     2401 acaatacttt ttcctttagc tgcgactaat tctttcgcaa ctttatctat tcctgtattt
     2461 aattctttat tactaaaatc ctttgcacca gaaacagaac cacctaactt agtatataaa
     2521 ttaattagaa cttgtttttc ttcagatggt ttaataggat atttataatc tgcacttgca
     2581 ccagtcaaac tcattgaaga ttgaaattga tggtggcgag acatcgttgg attttctggc
     2641 gtaggaactc tcttagttac atatttagca gcattttcag ttccattcat ccaagtacct
     2701 aagaaatcac agttaaaacc aacaattaca tctgctttat caaattggta agctggaata
     2761 attccgccac ttgcttttct gatagcatat tgtgaaattg ggtcataagc aactacacta
     2821 acatttgcgt aagttgtttt taattgctct attgcttttt tagtgatagt agataatatt
     2881 gtattcgtta ctacgactac tttttgacca gaagctttta actcatctaa ttttttaatg
     2941 atttcagcat ctgctttttt ccaatcaatt tttttattcg cttttacagg attatgtaat
     3001 cttgtaacgt catacaaatc taatacagca gcttgagctc ttgcacttgt tcctccgaaa
     3061 aattttgcag atggatttcc ttctacttta ataggtcttc cttctcttgt tttaactaag
     3121 atactgttaa aatcagcaga accataatag gttgttgcaa aaaactcagc aacaccagga
     3181 actacttccg gcatttggtt tcttaaatca aatgtgtaag gaattgcatt tttaacagga
     3241 atctgacatg cagacgcaac agcagcaact gaaacgctga atcccatagc ttttaagaaa
     3301 tctcttctgt tgggttcttt attttcaatg ctttcagtag catcttcaac taaactaaat
     3361 tcttggttag cttgctcaaa ataagcaggt gtttgattta actcatctaa actagtccaa
     3421 tactttttat tactcattat atatagaaat taataaggtt ctttaatttt aatagtgaca
     3481 tttttgacat tcagttccac ctatatcttc aacagtaact ccagctctct tgcctttctt
     3541 aatctcgtcg tgatattttt caaatactga ataataatta ttgtcagcaa actgaacttt
     3601 tgtttctcta tgacagttga tacaccagcc catagataat ggagcgaatt gttcaacagt
     3661 ttccatctct tcaattttac catgacaagt ttggcattca attttaccca cttttacgtg
     3721 ttgtgagtgg ttgaaataaa cgtggtcagg taaattatga atttgtaccc attcaatatt
     3781 tttaggtgct tttacaaatg ctcctttgtc aggatcccaa cctgaatagt catatatctt
     3841 tttgatttca tctttaccat attttggacc ttcttgaaca tacttatgac aattcataca
     3901 aatttgagta ccaggaatac ttgcatgacg gcttttttca actccagtat gacagtattg
     3961 acactcaatt ttattttgtc ctacgtgtaa tgcatgtgag aagcgaatag gttgttctgg
     4021 tttatatccc tgagaacgcc ctaagtttat tgcaccattt ccgatattaa aaagtaacaa
     4081 gactgctgca ataataactg cgactacacc taatttttta actaatgctt ttttagaggc
     4141 agcttcaaca gcttcgattg catctaaaga ttcgccttga gcaactttag caattttgct
     4201 taatttacta gtaatattaa acaatacagc tcctaaaata gctaaaacag caaataatcc
     4261 aactaggatt aatgttgtgt tagattttcc agaatctgca ccttcagttg tagcaccacc
     4321 agctgcatca gctgtttctt ttttaccgcc tggattacta gggtcgaagc ctttttcagc
     4381 tgctttcata taaaggaaaa catccttaac ttgctcgtct gataaagtag gaaatgccgg
     4441 cataggcatt ttattatatt cttcataaat cttattagca tgagcatgac cagatttaat
     4501 aacagcttga gaatttttta cccaagcaat taagttttct tcggtatccc atttatccca
     4561 aacatctgct aacgcaggtc cgattacttt tctatctata gcatgacaag ctgtacaatt
     4621 agccttgaac aagtccatac ctgtcttagc ttttgcaaca gcagcagcat cagcagggtc
     4681 aacgtcagga acggctaccc cagcataaga tgatgttgaa atcacaaaaa tcaagaaaaa
     4741 ggaaactaga aggctataac ttcttttaat ataactgtta aacattccta aaaagcgttt
     4801 taattatttt caggcaaata taaggtcaaa tagtttaaaa agtgaagatt tgttgaaaaa
     4861 atatatcaat ttatactcat tctagataac attaataagc tgaaatataa atacttagta
     4921 atctatttgg attatatctt aattgaattt aataaacact taacaacaac aaaagaaaaa
     4981 tcgttaatat ttctaaataa ttacagaaga attaaacttt aaaagaataa ttttgtgcat
     5041 catctgatta tggatattat tttaaaatac tttaaagaat taagcccaaa acaaatagct
     5101 caattcaaac aattattgca tttatatact gaatggaact ctaaaataaa tgtgatttct
     5161 aggaaggata tggagaactt ttatgaacgt cacgttttac atagtttaag tattgctgac
     5221 tttttcgatt taagtcatca tcaaatcatt gacattggta caggtggtgg atttcctggc
     5281 attcctttag ctatattttt tccagaaagt caattccatt tggtagattc aattggtaaa
     5341 aaaataagtg tggtaaatgc cattgcagaa gcattagaac ttaaaaatat aacagcacaa
     5401 catggaagag cagaacaatg caccaatttg tttgattatg cagtaacaag agccgttgct
     5461 ccagcagctg atttaattca ttggacatct aaaaatgtaa ataagaaaaa tggaatcatt
     5521 gctttgaaag gtggagactt gcgtgaagaa ttaaaagccg taaattgcaa aaaagaaatc
     5581 taccatttaa gtgctgttta ttccgaacct ttctttgaga caaaaaaaat aatacatcta
     5641 catcaaatca aatagattat ttcctaacta attttgcttc gtcaagcatc ttttgttttg
     5701 cagtagtttc atcttttaaa acagcaaaag taccttctcc agcattggca agaaattcta
     5761 attgcttaat aacataatct gttggttttc ctaatagaaa gatagaacag ttaatatttt
     5821 tttgagcgta atcggctaaa attttttcaa tatttttata ctctttagct cctgaagtaa
     5881 acataccatc tgtaaaaatc aaaattctat tatttccatt attgatataa ttttcagagg
     5941 ctttttgaaa tgcagatttt atagcagacg ctccatttgt tgtgcctcca acttttatta
     6001 atgatgcaat tgcattatga attttatctt tattatttcc attggttggt ggtaaaattt
     6061 cacttattcc agatgagaaa gtcaatagtg taattttatc ttctgatcta aaataatcaa
     6121 caatatcatt tagtccagat tttaaaactg gcaaaatatt attatctccc atcgatgagg
     6181 acatatcaac taagagtgtc caattattac tagcaaatcc ttctaatttt gataatggtt
     6241 ggacaactgt atctattgtc tttacaactt ctctcttatg ttttagtcca aaattatgtt
     6301 ccgaaatttc ttttactaaa acaacaccat tcaattcata ataatcaggg tgcgtaatat
     6361 tgatgtcata ttttccatca aggtatctaa caaaagtcgt aatattataa tttccagaag
     6421 aatcagtttt agcagtataa acatctgaat taaatcttag atttagagta gcaccaataa
     6481 ctggaatatt tttatctacg tccattattc ttccttgtat tatatttgaa taatgttgag
     6541 cgaataacga aaagctgata gcaaaaaaca aaattagtat tggtaatttt ttactcatat
     6601 cttttttata attcaaaatt attaaaaaca ttacaaaata cactatttta gttgaatgaa
     6661 taaaataatt tcttttctat ttctgatttt ttttggatta aatattattg ctcaaaatga
     6721 tacttatatc tcaaaataca aatacaaatg gggagttgat gctccaataa ttgggataac
     6781 agttggaact ggtgctgctg cttattttct gcacaagaat aagaaaataa ttactgttga
     6841 tgaaattaat gcactctctc caactatggt caataaattt gatagaagtg caatctttca
     6901 tgataataaa ggtgctaaaa tctcatctga tgttttatta tatggagcaa gtttggctcc
     6961 tgcattaatg tttattgata aggcggtaag aaaagaatgg aaatactatt tacctatgtg
     7021 gatagaagtt tacggtttga cttcgggttt aaccttgatg accaaagaaa ttgtgaaacg
     7081 taagcgacct tatgtttata atgataaact aagtgctgaa agtaaagttg gaaaagatgc
     7141 aactgcatca ttcttttcag gacatacaag tacgaccgca gctagtacat tttttatggc
     7201 aaaagtattt gcagatttac atccagattc taaatttaag ccacttatgt ggactggagc
     7261 ggcattaatc ccagcaataa caggactgtg tagatatggt gcgggtaagc atttctttac
     7321 tgatatttta gtgggctatg ccattggttc tgctgttggt attttagttc cacagttaca
     7381 taaaagaaaa attcaataaa acaaaagagg ctgtccaaag ttttaggaca gcctctttct
     7441 ataataaaac attaaaaatt aaaacttatt attctcagcg attaattctg ctaagtccac
     7501 aactttaatt tcgtcttgtt tgttttcact ttttacaccg tcagtaatca ttgtattgca
     7561 ataagaacaa gccaccgcaa taatatcagg cattacttca gttaattgac ctgttctttc
     7621 ggtattgata cgttttgttc ctggttcatc ttctttccac atttgagctc caccagcacc
     7681 acaacacatt gcgtttgctc tgcttttggt tacttcaacc aaatctgcat ctaatgcttt
     7741 aattaaatct cttggagctt cataaatatc attagctctt cctaagtaac aagaatcgtg
     7801 gtaagttatt cttttacctt taaaagctcc gccttctact tttaatttac catcatttaa
     7861 taattgttgt aaatatactg aatggtgtaa tacttcatat tttccaccta acgcaggata
     7921 ttcattttta atggtattaa aacaatgcgg acaagtggta acaatagtct tgataccata
     7981 accatttaag gttgcgatat tttgttgtgc caacatttga aaaataaatt cattaccagc
     8041 tcttcttgct ggatcgcctg tacaagcttc ttctttgcct aatactgcaa aactgatatt
     8101 taattgattt aaaatcttca cgaatgcttg tgtaattctt tgagcccttt ggtcaaaact
     8161 acctgcacat cctatccaaa ataatacatc tggagattta ccttcggcag ccaaatctgc
     8221 cattacagga actttatatt gcatattgag tattgttgtt agttattaaa atattgattt
     8281 actttattat ttattcaaat cgtcagtcca attcaatcta tcttccattg agaactgcca
     8341 tggtgcttga ttattttcca tattagcaaa catcgtattc cattcttgtg gtgaattact
     8401 ttcgtccata atcatattcc ttcttaattc taagatgata tttaatggag aaatattaac
     8461 tggacattct tcaacacatg cattacaagt cgtacaagct cttaactctt cgcttgtaac
     8521 ataatcattc aataaggttt tattatctgt aaaatctgga ccatgaattt ttctatttct
     8581 tgtaatttct tccattctat cacgcgtatc aatcatgatt tttcttggag ataatatttt
     8641 acctgtttgg tttgctggac aagcttgagt acaacgtcca cactctgtac aagtataagc
     8701 atctaataaa cttttccatg ataaatcaaa cacatcttta gcaccgaatt taatttcacc
     8761 atctgtttca ggtgcttgat aagaatcatc aaacatggat tttacttcat gcataacgct
     8821 ttccatatta cgaatttgac ctttaggttc tggtcttgaa aagaaagtat ttgggaatgc
     8881 caataagata tgtaagtgtt tagaataagg caaatacaac ataaaagcaa ataccatact
     8941 taaatgtccc caccaaccaa ttctttccat catcactaaa gcaccgtgcg gtaaactcat
     9001 caggctatga cctaaaaaat tacttaataa aaagcctttt gtatcgatat aatgtgggtc
     9061 tccttgtaat tgcaatgctc tatctgcacc attcatcata aagatgaatg tgattaaagt
     9121 taattcaaaa gctaagataa gtaaaccatc ttttgtaggc caacctttca tttctggctt
     9181 attaaatcta ttaattttaa aaactgttct tcttaaaata aatactatcg taacgaagaa
     9241 agtcaataaa gataatacct caataaaact aactacaaaa cgataaaatc cttctaaaat
     9301 attaccttgg aaaatataat gaaataaaat tctatgctgt ccagttgctc catctaacat
     9361 aatctcaatc aactctatct gcgtaatgac aaaagataaa taaataacca agtgcaacaa
     9421 tgctggaaag gtaattttaa acattttctt ctgtccaaaa gcaattaaga acatattttt
     9481 tagtctaaaa cttaatggtg tatcaattgt ttctggctga cccattttga tattgtcata
     9541 aacctctttg aattttttga aggcaaaata tcctactgtt ccagttaagg cgtagaataa
     9601 aatcatctga ataattgtta aaaacatttt tttggaaaat tttgactaaa atacgatgtt
     9661 tgaacgtatt ctgcaatatt tttttaacga aaagccttat tttataatga ttctaaataa
     9721 tctatttgtc gctaaagtgt caataaaatc aagtaaaaat gaatattatc cattaaatat
     9781 atacaaatca atacatgact attgctcaat tgatatttat aatctaatca attcttaatg
     9841 ttaatatgaa taactatttt aaaaggattt tagaaataaa aatagctgca ctaaggcagc
     9901 cattttataa ttagttgttt gctattcaaa agtttaactc aacttacgtt ttacttctac
     9961 ttgttcaaat gattcgataa tatcgccttc tctaatatcg ttgtagtttt taattttgat
    10021 accacattca taacctttag ttacttcttt cacatcatct ttgaaacgtt ttaatgaatc
    10081 taaaccacct tcaaacacta caataccatc tctaataata cgaacctcgt tagaacgaac
    10141 tattttacca tctgtaacgt agcaacctgc gatagtacca attttactca ctttaaacac
    10201 ttctctaacc tcgacattac ccacaatttt ctcttcaaat ttaggttcta ataaaccttc
    10261 cattgccgat tttacttctt caattgcact ataaataaca gaatacatct taatttgtat
    10321 atcttctttt tctgcaatta attttgcatt tgcagatggt ctaacttgga atgcaataat
    10381 aatcgcatct gatgcagaag ccaataatac atctgattca gaaatttgac caacaccttt
    10441 atgtatgata ttgatttgaa cctcttctgt agataatttc aataaagagt cactcaatgc
    10501 ctccactgaa ccatcaacat ctcctttgat aattaagttt aactctttga agtttcctaa
    10561 agctaaacgt cttccaattt catctaa
//
LOCUS       DAOQCW010000091        10569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_2348_length_10569_cov_5.960814, whole genome shotgun sequence.
ACCESSION   DAOQCW010000091 DAOQCW010000000
VERSION     DAOQCW010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10569
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2348_length_10569_cov_5.960814"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PLS16_08585"
     CDS             <1..462
                     /locus_tag="PLS16_08585"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNJ61176.1"
                     /translation="STRAKYLPRLNAFGSFEFHDHKFARFRANNYLVGAQLQWQIFDG
                     LKAKSEQVAYKANQIKIKSEIEQYTQQSELELKKISRQIEDAEYKVKFAVSALEQSKE
                     AYRIRKDRYEQGLEKSSDLLNAEIMMSQKHLEYYQAVFEYNTALAYYNFLK"
     gene            483..1583
                     /locus_tag="PLS16_08590"
     CDS             483..1583
                     /locus_tag="PLS16_08590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNJ61177.1"
                     /translation="MRKNRIIQGVALILSMLMFTYCSNSETSKNIEANGETVKVTLSQ
                     TEENTSGSNATASGRLVSKNSVNVSTRMMGYITSMSVKVGQSVKAGQLLVTINNADIQ
                     AKGGQVDAQIMQAQANYDIAKKDFERFQNLYNNQSASQKELDDMKARFEISKAGLEAS
                     KQMKNEVNAQYRYTNLTAPISGVVTMKFAEQGDLANPGMPLLTIESPSILQAQVMVSE
                     QNIIQVKQGMQVKVVIKSTGKEITGTVAEINQSAEYTGGQYIVKINMPTSSELLPGMY
                     VNARFPFESKSVQLAENSSSVVIPKSALIRNGQMTGVYTVSSQNTAILRWLKLGKDLG
                     NDIEVLSGLNANEAYILSADGKLYNGVKVSTK"
     gene            1643..4852
                     /locus_tag="PLS16_08595"
     CDS             1643..4852
                     /locus_tag="PLS16_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNJ61178.1"
                     /translation="MDKGIAGRLADMFLDSKLTVLLMVALMIIGVYSSSLIPREEEPQ
                     IIIPMADVMVGYPGASPKEVESRVVKPLEKIISNIKGVEHIHSMAMNGQAMLIVQFLV
                     GEDIERSYVKLYDELMKNKDIFPGGVYPPMVKTRSIDDVPMLGLTLWSEDYDDFQIRQ
                     IGEEVASGIKKIKDVSLTNIIGGRTRQLKIVLDKEKMAELNVDALSVMQMIQASNNSS
                     QSGSFNANDTEYLLTTGQFLSTAEDVENLVIGTAQNMPVYLKQVAKVEDGPSSAKNYV
                     NFGYSNAVENSKQFSSEYPAVTLSVSKVKGADAMKISEQILAKVEEMKKDLIPNNVHV
                     EVTRNYGETASHKVSELLIHLAVAIIAVTVLVMLAMGWRGGLVVFLSVPLTFALTLFS
                     YYVLGYTLNRITLFALVFVVGIVVDDSIIIAENMHRHFHMRKLPFKQAAIYAINEVGN
                     PTILATFTVIAAILPMAFVSGMMGPYMSPMPIGASIAMLLSLFIALTVTPYLGYHLLK
                     VKDDQEHKEEEGLETNAIYKIYKKLEMPLLENRGRRNLILGSTLILLIISMAAFGFKW
                     VAVKMLPFDNKNEIQLVIDMPEGTTLERTAAVTQDIAQYIKTIPEVVNYQSYIGSSAP
                     ITFNGLVRHYDMRGASNTADIQVNLKHKEDRKLQSHDVAKNIRPAIQKIAAKYGANVK
                     VVEVPPGPPVLSTLVAEIYGPDYDEQIKIARQIEQILHNTDDVVDIDTRIEDEQKELK
                     LVFDKEKSMLNGVAPMQIVGNMTYLMGEHPIGNLYDERSNRPVNIVMKLSDADKTSIE
                     DITNMKVKGQMGMVPVSDLVKVQEDVLQKSIYRKDQQRVVYVMADMAGGLESPAYAIL
                     GMSKKIQKMDIPKGYKVEEMYLGQPESEENYTVKWDGEWQITLEVFRDLGMAFMVVMI
                     IIYMLIVGWFQNFKTPIVMMIAIPLSLIGIVLGHWLLGAFFTATSFIGMIALAGVMVR
                     NSVLLIDFIEIRLNQGIPIKQAIIEAGAVRTTPILLTTGAVVIGAAIILFDPIFQGLA
                     ISLVFGAIVSTMLTLLIVPVIYYISEHKKWEKNEKNKAI"
     gene            4872..5174
                     /locus_tag="PLS16_08600"
     CDS             4872..5174
                     /locus_tag="PLS16_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61179.1"
                     /translation="MKLLLITSIQEYENDVKDILRHSGVKSFSYQEVKGFKNEHNGSA
                     TNWFGLTDIPTESILFTVFIETSCIDEIFELVEKFNAERTSLSKIHISCIAVDKSI"
     gene            5197..5412
                     /locus_tag="PLS16_08605"
     CDS             5197..5412
                     /locus_tag="PLS16_08605"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNJ61180.1"
                     /translation="MYNRIAHGFAGTLIITSLVLTHFTGNNNWLWIAAFVGVNLFQNS
                     LTNWCLLHQILKAVGIKDENGSCSTNS"
     gene            complement(5513..6244)
                     /locus_tag="PLS16_08610"
     CDS             complement(5513..6244)
                     /locus_tag="PLS16_08610"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNJ61181.1"
                     /translation="MLSINEVKHINSLKIKKYRNKYNEFIAEGKKVIIELIQNQFICK
                     QLYIDETLHNNLDLPDNIHIKTIHETDFKKISNQKNPSNILAIFEKPQQKTIQKIDNW
                     LIALDNIQDPGNLGTIIRIADWFGIDTIVCSEDCVDVYNSKTIQASMASIANINIIET
                     DLAEFFKQHPNIPIFGAILDGKDYNQIQYPEKGILLIGNEGKGIQTELLKTITQTITI
                     PRHGKAESLNAAIATSIIVAKIKAN"
     gene            6287..8707
                     /locus_tag="PLS16_08615"
     CDS             6287..8707
                     /locus_tag="PLS16_08615"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNJ61182.1"
                     /translation="MIVALQSCRATKYVLKDNESLLVANKIMLHSDIKNIDDKSKMQT
                     DLARQVIYNQQPNKKFMNFFRLKLGLYAASYRKKNKPKNESKKKKKGDKAFFEDYAGE
                     PPVIFDSAAIESSKARMSNYLFYKGYFHSNISTDFETKNKKTVVTYHIDTDTAFRFRK
                     IIKQTEDSILYKYANLDNGNSFLKEGDIFDIDNIKKERERIATNIQNKGYFTFSPDFI
                     VLRVDSTNAGEHLVDAYLVVKNEVDSSLHKRYVYLDINVNVNHDNKSKSKKATFLERQ
                     LDTICNVNYNVAKNSIKPKILSRSLRLKPEDIFSVNEQVATRSNLYGLGVFNFINIKH
                     EPFSFSPEEMGLITSIECVMSKKHSFSNELELNTNAQSTLGFSLSGTYINKNVFNSAA
                     KLYVSLRSGVNFQFQKNFQDGEHLSAINNVNVLGEVRISLARIFPSFKRKQCTTYEKY
                     KPQTNIALNYNYQRRVGLYTLNTISINYGYEWYNDRFRHIFSPLSLTYVKPSNLTAAF
                     NDRLQNNPFLKKSFDNQFILGQDYTFFYTNQNINVGKFKNYFSLRANFNMGGNILFAF
                     ASLNKNKTKPYSLSNIPFAQFARIELEPKYFFNFKKKQSLGFRAFVGIGIPYGNSSTV
                     MPYIKQFSAGGPMSLRAWPYRQIGPGGYKFRNTNTNIAQLDQTGDIRLELNAEYRFNI
                     FKMFKGAVFSDAGNVWTYKKDTARTNAEFNFKRFGKEIAWCVGAGLRLDLSFFVIRFD
                     VALPIYDPSYTSGAKWVSDIIGYRRDYFNQKYEANPTMTHKEVRKYYRRTEIGRMVGY
                     NIAIGYPF"
     gene            8715..>10569
                     /locus_tag="PLS16_08620"
     CDS             8715..>10569
                     /locus_tag="PLS16_08620"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNJ61183.1"
                     /translation="MRFYLLLFYFFGSFSSLLFAQNLKLKPIHSIEVEENGHSLKMPF
                     VGGLTSVQVAKLDMNNDHQTDLVIYDIYSNRFIVLTNNEGAYLYDTTQYSFPNAYGWV
                     EWKDFNCDNIKDFVTYNNLGSIEIHKGFYTNNSLQFEKMQNNVLAKSFFGDVNVYASS
                     IDKPSFIDVNGDGDIDMVNYNVSMSKLMYYENQQVEKNIPCDSVYFEIADFCWGNITE
                     NGHGTLSFRDTCNSKFLTPLGSEEIQHNGTAVCLYDVNGDTIKDALLSDLFFNSVNYL
                     ENVGSKYYASFLSENKNYPASYPIEIDYLPMPQFVDIDDDNKDDCIVSNFSFTSSDAN
                     ADNIWWYKNVSSNSSLQLEFQKKNFLLEDVIDVGQQAYPCLLDINNDGIKDLLISNGG
                     QRKSGEQNKYFVKTYQNLGTNKHPKYNLVDTNFLNISQYGLTEIALTVGDINNDGVDE
                     ILAGTSDGFLVWWENISMSPISEWIYRGYLLKDVFGNPDTLGANVVPIIYDLDNDNLK
                     DLIIGKKSGTLSFFKGNAGTTPNFTKITTQLSNLQTNKNNEFWGYSVPFIADIDTNGI
                     DDLILGTYAKGIIWYPDFVNNYTQANLEEKVLLESKFYRTIPVILENNIFLV"
BASE COUNT         3775 a         1398 c         1942 g         3454 t
ORIGIN      
        1 tcaacaaggg caaaatattt acctagatta aatgcatttg gaagctttga atttcatgac
       61 cataagtttg caagatttcg tgctaacaac tatttagttg gggctcaatt gcaatggcaa
      121 atttttgatg gattaaaagc aaaaagtgag caagtagcct acaaagctaa tcaaataaaa
      181 atcaagtcag aaatagaaca atatacacaa caaagtgaat tagaattaaa gaaaataagc
      241 cgacaaatag aagatgcaga atacaaagta aaatttgctg tatctgcttt agagcaaagc
      301 aaagaggcat atagaattag aaaagacaga tacgagcaag gcttagaaaa gtcttctgat
      361 ttattgaatg cagaaatcat gatgtctcaa aagcatttag aatattatca agctgttttt
      421 gaatacaaca cagcattagc atattataac tttttaaaat aaacaatcaa aaaaatacaa
      481 gcatgagaaa aaatagaata atacaaggtg tagctttaat cctttctatg ttgatgttta
      541 cttattgttc caatagtgaa acatcaaaaa acattgaagc caatggagaa actgtcaaag
      601 taactttaag ccaaacagaa gaaaatacaa gtggctcaaa tgcaacagca agtggtcgtt
      661 tggtttctaa aaattcggtt aatgtttcaa caagaatgat gggttacatt acatcaatga
      721 gtgtaaaagt aggtcaaagc gtaaaagcag gtcaattatt ggtaacaatc aacaatgcag
      781 atattcaagc aaagggtggg caggtagatg cacaaattat gcaggcacaa gcgaattatg
      841 atattgcgaa aaaggatttt gaacgtttcc aaaatttata taataatcaa agtgcgtctc
      901 aaaaagaatt agacgatatg aaagctcgtt ttgaaatttc aaaagctgga ttagaagctt
      961 caaaacaaat gaaaaatgaa gtcaatgcac agtatagata tacaaatctg actgctccaa
     1021 tttcgggcgt tgtaactatg aaatttgcag aacaaggaga tttagccaat cctggaatgc
     1081 cattattgac gatagaatct ccatcaattc tacaagctca agtgatggtt tctgaacaaa
     1141 atatcattca agtaaagcaa ggaatgcaag ttaaggttgt gattaaatcc acaggtaaag
     1201 aaattacagg aacagttgca gaaatcaatc aatctgcaga atatacaggt ggtcaatata
     1261 ttgtaaaaat caatatgcca acttcatctg aattattgcc aggaatgtat gttaatgctc
     1321 gtttcccttt tgaaagcaaa agtgttcaat tagctgaaaa ctcatcaagt gttgtcatac
     1381 caaaatctgc tttgattaga aatggacaaa tgacaggtgt ctatactgta agttctcaga
     1441 atactgccat tttgcgttgg ttaaaactag gaaaagattt aggcaatgat attgaagtat
     1501 tgtctggtct gaatgctaat gaagcctata ttctttcagc agatggaaag ttgtataatg
     1561 gtgttaaagt gagtactaag taatgattga aaacaataga acaagcatct attgatttta
     1621 aaactgtaaa aaaagagata aaatggataa aggtatagca gggcgtttag ccgatatgtt
     1681 tttagactct aaactgacgg tgcttttaat ggtagcattg atgattattg gagtatatag
     1741 ttcttctttg attccaagag aagaagaacc tcaaattatt attccgatgg cagacgtaat
     1801 ggtcggctat ccaggagcta gtcccaagga agtagaaagt agagtagtaa agcctttgga
     1861 gaaaatcatt tccaatatca aaggtgtgga acatattcac tctatggcaa tgaatggaca
     1921 agcaatgtta attgtacaat ttttggtagg agaagatatt gaacgctctt atgtcaaatt
     1981 gtatgacgaa ctaatgaaaa acaaagatat ttttccaggt ggtgtctatc ctccaatggt
     2041 aaaaactcgt tctatagatg atgttccgat gttaggtttg actttatgga gtgaggacta
     2101 tgatgacttt caaatccgcc aaattggtga agaagtagca tctggtatta aaaagataaa
     2161 agatgtatct cttacaaaca ttataggggg aagaactaga caattaaaaa ttgttttaga
     2221 caaagaaaaa atggctgagt taaatgtcga tgcattaagc gtaatgcaaa tgattcaagc
     2281 tagcaataat agttctcagt ctggaagttt taatgccaat gatactgaat atttattaac
     2341 aacaggtcaa tttttaagta cagcagaaga tgttgaaaat ttagttattg gaacagctca
     2401 aaatatgcct gtatatttaa aacaagtcgc aaaagtagaa gatggaccat catcagcaaa
     2461 gaactacgta aattttggtt atagcaatgc agtagaaaat agtaagcaat tttcatcaga
     2521 atatccagca gtaacacttt ctgtttctaa ggtaaaagga gcagatgcaa tgaaaatctc
     2581 tgaacagatt ttagcaaaag tggaggagat gaaaaaggac ttaattccta ataatgttca
     2641 tgtagaagta actcgaaact atggagagac agcttctcac aaggtatctg aattattaat
     2701 acatttagca gtagccatta ttgcagttac agttttagtg atgttagcaa tgggctggcg
     2761 aggtggttta gtggtattct tatccgttcc attgacattt gcattgacat tatttagtta
     2821 ttatgtttta ggctatacct taaacagaat tacattattt gctttagttt ttgtggtagg
     2881 tatcgtggtg gacgacagta ttattattgc tgagaatatg caccgccatt ttcacatgcg
     2941 aaaacttcct ttcaaacaag ctgcgattta tgccattaat gaggtaggaa atccaacaat
     3001 actagccaca tttacagtaa ttgctgcaat tctgccaatg gcatttgttt caggaatgat
     3061 ggggccatat atgagtccga tgccaatcgg tgcatctata gctatgttat tatctttatt
     3121 tattgccttg acagtaacgc cttatttggg ttatcattta ttaaaagtaa aagacgacca
     3181 agaacacaaa gaggaagaag gattagaaac caatgcaata tataaaattt ataaaaaact
     3241 agaaatgcct ttgttggaaa atagaggtag aagaaattta attttaggta gtacgcttat
     3301 tttgctaatt atttctatgg ctgctttcgg attcaaatgg gtggctgtaa aaatgttgcc
     3361 ttttgacaac aaaaatgaga ttcaattagt gattgatatg cctgagggaa caaccttaga
     3421 aagaacggca gctgttacac aggatattgc acaatatatc aaaacaattc ctgaggttgt
     3481 aaattaccaa agttatattg gttcatctgc tcctattact tttaatggat tggtacgcca
     3541 ctatgatatg agaggtgcta gcaatactgc tgacattcaa gtaaatttaa aacataaaga
     3601 agatagaaaa ttacaaagtc atgatgtggc taaaaatatt cgtcctgcta ttcaaaaaat
     3661 tgcagcaaaa tatggtgcta atgtaaaagt agttgaagtg ccaccagggc cacctgtatt
     3721 atctactcta gttgcagaaa tttatggtcc agattacgat gagcaaatta aaattgctag
     3781 acaaatcgaa caaattcttc ataatacaga tgatgttgtg gatattgata caagaattga
     3841 agatgaacaa aaagaattaa aattagtatt cgacaaagaa aaatcaatgc tgaatggtgt
     3901 tgctccaatg caaattgttg gcaatatgac ttacttaatg ggagaacatc caataggtaa
     3961 tttatatgat gagcgttcta accgtccagt taatattgtc atgaaattaa gtgatgccga
     4021 caaaacatct attgaggaca ttaccaatat gaaggttaag ggacaaatgg gaatggtgcc
     4081 tgttagtgat ttggtgaaag tacaagaaga cgttttacaa aaatctattt atagaaaaga
     4141 ccaacaaaga gtggtttatg tgatggcaga tatggctggt ggattagaaa gcccagctta
     4201 tgcgatacta ggaatgagta aaaagataca aaaaatggat attccaaaag gctataaagt
     4261 agaggaaatg tatttgggac aaccagaaag tgaagaaaac tataccgtaa aatgggatgg
     4321 agaatggcaa attaccttag aagtattccg tgatttaggc atggcattta tggtcgtaat
     4381 gattataatc tatatgctaa ttgttggatg gttccaaaat ttcaaaactc caattgtgat
     4441 gatgattgcg attccattat ctttgatagg tattgtttta ggacactggt tattaggagc
     4501 gttctttaca gcaacatcat ttattggtat gattgcatta gcaggtgtga tggttcgaaa
     4561 ttcagtattg ctcattgact ttattgaaat tagattaaat caaggtattc caattaaaca
     4621 agctattatt gaagctggag cagtgcgaac aacacctatt ttattgacaa caggtgcagt
     4681 agttattgga gcagccatca tcttatttga cccaattttt caagggttgg caatttctct
     4741 agtatttgga gctattgttt ctactatgct tactttattg atagttccag tgatttatta
     4801 catctctgag cataaaaaat gggagaaaaa cgaaaaaaac aaagcaattt aaaaatctaa
     4861 aatagctaac aatgaaatta ttattaatca caagtattca ggaatacgaa aacgatgtca
     4921 aagatattct aagacattca ggtgtaaaat ctttttctta tcaagaagta aaggggttta
     4981 aaaatgaaca caacggaagt gctaccaatt ggtttggatt aactgatatt ccaactgaat
     5041 caattctatt cacagtgttt attgaaacta gctgtataga tgaaatattt gaactagtag
     5101 agaaatttaa tgcagagcgt acatctttat ctaaaattca tatttcatgt atagctgtag
     5161 ataaatctat ttaatcaatc tttaaaaaca aataatatgt ataacagaat agcacatggt
     5221 tttgcaggta ctctaattat aacaagttta gtactcacac attttactgg aaacaacaat
     5281 tggctttgga ttgcggcatt tgtaggagtg aatttatttc aaaactcact aacgaattgg
     5341 tgtttattac atcagatttt gaaggcagta ggaataaaag atgaaaatgg tagttgtagt
     5401 acaaatagct aagttattaa aatgataatg aggattaaaa ataaatgagt aacgaagaag
     5461 attgaaggaa tttggcttga gatttatgaa taatgattgc cttgcaaggt tcttaattag
     5521 cttttatttt cgcaacaatt atgctcgttg caatagcagc attcaaggat tctgcttttc
     5581 catgtcttgg aatagttatg gtttgcgtga ttgttttaag aagctcagtt tgaatgcctt
     5641 taccttcatt tccaatcaat aaaatgcctt tttcgggata ttgaatttgg ttataatctt
     5701 tgccatctaa gattgctcca aaaataggaa tatttggatg ttgcttaaaa aactcagcca
     5761 aatcagtttc aataatatta atattggcta ttgatgccat agaggcttgt attgttttgc
     5821 tattgtaaac atcaacacaa tcttctgaac aaacgatggt atcaatgcca aaccaatcgg
     5881 ctattctaat aattgtacca aggttgccag ggtcttgaat attatctaag gcaattaacc
     5941 aattgtctat tttttgaata gttttttgtt gtggcttttc aaaaatagct aaaatattag
     6001 aaggattttt ctggttggat attttcttaa aatcagtttc atgaattgtt ttgatgtgga
     6061 tgttatcagg taagtctaaa ttgttatgta gagtttcgtc aatatatagt tgtttacaaa
     6121 taaattgatt ttgtattaat tctattataa ctttcttacc ttcggcaata aattcgttat
     6181 atttattcct gtatttttta attttgagag aatttatatg tttgacttcg tttatactaa
     6241 gcataaacca caattaaaat ttctgacaaa attagttttt attttgatga tagttgcatt
     6301 acaatcttgt agagcaacaa aatatgtact gaaagataat gaatctttat tggttgctaa
     6361 taaaattatg cttcattctg atataaaaaa tattgatgat aagagcaaaa tgcaaacgga
     6421 tttagcgaga caggttattt acaatcaaca acctaataag aagtttatga atttttttag
     6481 gttgaagctt ggattgtatg ccgcatctta tagaaagaaa aataaaccta aaaacgaaag
     6541 caaaaaaaag aaaaaaggag ataaagcatt ttttgaagat tatgcgggtg aaccaccagt
     6601 tatttttgat tcagcagcaa ttgaaagttc aaaagctaga atgagtaatt atttgttcta
     6661 taaaggttat tttcatagca atatttctac tgattttgaa actaaaaaca aaaaaactgt
     6721 tgttacttat cacattgata ccgacacagc atttagattt agaaaaatta tcaagcaaac
     6781 tgaagattca attctttata aatatgccaa tttagataat ggtaattcct ttttgaaaga
     6841 aggagatatc tttgatattg ataatattaa aaaagagaga gaacgtattg ctaccaatat
     6901 ccaaaacaaa ggttatttta catttagtcc agattttatt gtgttgcggg tagatagcac
     6961 caatgctggc gaacatttag tagatgctta tttggtggta aaaaatgaag ttgattcttc
     7021 attgcataaa agatatgttt atttagatat caatgtgaat gtaaatcacg ataacaaatc
     7081 aaaatctaag aaagcaactt ttttagagcg tcaattggat acaatttgca atgtgaatta
     7141 taatgttgcc aagaattcta taaaacctaa aatattatct cgttcgctta gattgaaacc
     7201 tgaagatatt tttagcgtaa atgagcaagt tgccacacgt agtaatttat atggattagg
     7261 cgttttcaat tttattaata ttaaacacga accattttca ttttctccag aagaaatggg
     7321 tttgattact tcgattgaat gtgtgatgag taaaaaacat tcctttagta atgaattgga
     7381 attaaataca aacgcacaaa gtacacttgg cttttcttta tctggcacat atatcaacaa
     7441 aaatgtattt aattctgctg caaaattata tgttagttta agaagtggtg taaatttcca
     7501 atttcaaaaa aactttcaag acggagagca cctaagtgcg ataaataacg tcaatgtttt
     7561 gggtgaagtg aggattagtt tagcacgtat ttttccatct tttaaaagaa aacaatgtac
     7621 gacttacgaa aaatataaac cgcaaacgaa tattgcattg aattataatt atcaaagacg
     7681 tgttggttta tatacactta atacaatatc tatcaattat ggctatgaat ggtataatga
     7741 tagatttaga catatctttt ctcctttgtc gcttacctat gtaaagcctt ctaacttgac
     7801 tgctgctttt aatgatagat tgcaaaataa tcctttttta aagaagagtt ttgataatca
     7861 atttatttta ggtcaagatt atactttctt ttataccaat cagaatatta atgttggaaa
     7921 gtttaagaat tatttttcat tgagagccaa ttttaatatg ggtggaaata tattatttgc
     7981 atttgcttca ctcaataaaa acaaaacaaa accttattca ctttctaata ttccatttgc
     8041 acagtttgca agaattgaat tagagccaaa atactttttt aattttaaga aaaagcaatc
     8101 acttggattt cgtgcatttg taggaatagg tattccttat ggcaattcat ctacagtaat
     8161 gccttatatc aagcagtttt cggcaggtgg cccaatgtct ttgagagctt ggccatacag
     8221 acaaattggt cctggcggtt ataaattcag aaacacaaat accaatattg cacaattaga
     8281 ccaaacggga gatattcgtt tggaattgaa tgctgaatat cggtttaata tttttaaaat
     8341 gtttaagggt gcagttttct ctgatgctgg aaatgtttgg acgtataaaa aagatacagc
     8401 gagaacgaat gctgaattta attttaagcg atttggcaag gaaatagctt ggtgtgtggg
     8461 agcggggctt cgtttagacc tatccttttt tgtcattaga tttgatgtag cacttccaat
     8521 ttacgaccca agttacactt ctggagccaa atgggtttca gatattatag gttatcgtag
     8581 agattatttt aatcaaaagt atgaggctaa tcctactatg acacacaagg aagtacgaaa
     8641 atattataga agaacagaaa ttggcagaat ggttggttat aatattgcaa ttggttatcc
     8701 tttctaattt ttttatgaga ttttatttac tactttttta tttctttggc agtttttctt
     8761 ctttgttatt tgcacagaat ttaaagctga aacctattca ttctattgaa gttgaagaga
     8821 atggacattc gctgaaaatg ccttttgttg gcggattaac aagtgttcaa gttgctaaac
     8881 tagatatgaa taatgaccat caaacagact tggtaattta tgatatttat tccaatcgtt
     8941 ttatagtttt gacaaataat gagggtgcgt atctttatga caccacccaa tattcctttc
     9001 caaatgctta tggttgggta gaatggaaag attttaattg tgataatata aaagattttg
     9061 taacctataa taatttaggt tctatagaaa ttcataaagg attttatact aataacagtt
     9121 tgcaatttga gaaaatgcaa aataatgtat tggctaaatc attttttgga gatgtaaatg
     9181 tctatgcaag ttcgatagac aaaccatctt ttattgatgt gaatggagat ggtgatattg
     9241 atatggtgaa ttataatgtc agtatgtcta aattgatgta ttatgaaaat caacaggtag
     9301 agaaaaacat accttgtgat tccgtatatt ttgaaatcgc cgatttttgt tggggaaata
     9361 ttacagagaa tggacacgga actttgagtt ttagagatac ttgtaatagt aaatttttaa
     9421 ctcctttagg ctcagaagag atacaacata atggtacagc agtttgcttg tacgatgtga
     9481 atggcgatac gattaaagat gctttattgt ccgatttgtt ttttaattct gttaattatt
     9541 tagaaaatgt agggtcaaaa tattatgcaa gttttttgtc tgaaaataaa aactatccag
     9601 cgagttatcc aatagagatt gattatttgc ctatgccaca gtttgttgat attgatgatg
     9661 ataacaaaga tgattgtatc gttagtaatt tttcttttac atcttccgat gctaatgcag
     9721 ataatatttg gtggtataaa aatgtatcta gcaattcaag tttacagtta gaatttcaga
     9781 aaaagaactt tttattggaa gatgtgatag atgttggaca acaggcttat ccttgtttgt
     9841 tggatatcaa taacgatggt attaaagatt tgttgattag taatggagga caaaggaaaa
     9901 gtggagagca aaacaaatat tttgtcaaaa cttatcaaaa tttaggaacc aataagcacc
     9961 caaaatacaa tttggtagat acaaattttc taaatattag ccaatatgga ttaacagaaa
    10021 ttgctttgac agttggggat ataaataatg atggtgttga tgaaatactc gcaggtactt
    10081 cagatggatt tttagtttgg tgggaaaata tttctatgtc tccaatttca gaatggatat
    10141 atagaggtta tttattaaaa gatgtatttg ggaatcctga tactttaggt gcaaatgtag
    10201 ttcctattat ttatgactta gataatgata atttgaagga tttaattatt ggtaaaaaat
    10261 caggtacact tagttttttt aaaggaaatg ctggaacgac acctaatttt acaaaaataa
    10321 cgacacaact atcgaattta caaaccaata aaaataatga gttttggggc tattctgttc
    10381 catttattgc cgatattgat acgaatggaa tcgacgattt aatacttgga acttatgcaa
    10441 aaggaattat ttggtatcct gattttgtaa acaactacac acaagccaat cttgaagaaa
    10501 aggttttatt agaaagcaaa ttttacagaa caattccagt tattttagag aataatatat
    10561 ttctagttg
//
LOCUS       DAOQCW010000092        10340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_2437_length_10340_cov_5.374818, whole genome shotgun sequence.
ACCESSION   DAOQCW010000092 DAOQCW010000000
VERSION     DAOQCW010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10340
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2437_length_10340_cov_5.374818"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..927)
                     /locus_tag="PLS16_08625"
     CDS             complement(73..927)
                     /locus_tag="PLS16_08625"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNJ61184.1"
                     /translation="MAEIKKKPEAKIRKVNFTFEKPFPKHWYNDNPIATHFMNAQHLV
                     FPDGEKFFIRSVKAFADVYKNDPELKKRVDNFIGQEGTHFAQHQKFWDVIEEHGLRPD
                     KFLNIYRNITWNGIESFARKTLKKNRLGDKICLSITVGLEHFTAMLAESGLLDKDVTK
                     KMPAEMKELFMWHAAEEIEHKAIPFDVLKAVDDNYWLRIGGMAIATWGLWSFILLGTA
                     MLTAQDEDVKAKDIPGYILSFIKKFNENVGGTLSAQFSQYFQKDFHPDQIENYHLAEE
                     LLAGKNYA"
     gene            complement(1002..1955)
                     /gene="fmt"
                     /locus_tag="PLS16_08630"
     CDS             complement(1002..1955)
                     /gene="fmt"
                     /locus_tag="PLS16_08630"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNJ61185.1"
                     /translation="MVEKEDLRIIFMGTPEFAVASLDILVKNNYNIVGVVTATDKPAG
                     RGNKLQTSAVKQYAEANQLKVLQPEKLKSPEFIEQLKALKPNLFVVVAFRMLPEIVWD
                     MPQFGTFNLHASLLPQYRGAAPINWALINGEKETGVTTFFLQHEIDTGNIIFQDKIQI
                     TEQDNVGSVHDKLMQIGAELVLKTVQAVEKNEVQSYAQISNLQSSNLILQHAPKIFKE
                     TCLINWNNSAENVQNLIRGLSPYPAAFTYLDNKTLKIYSSIINEERNLIDTSIGNFYT
                     DGKTFLSFRAADTFIDILELQLEGKKKMTVQEFLRGHRFNL"
     gene            2015..>2538
                     /locus_tag="PLS16_08635"
     CDS             2015..>2538
                     /locus_tag="PLS16_08635"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNJ61186.1"
                     /translation="MKKYVLVVLLFFCFHAVFAELEFNVGGKVGVNLSHIRKMDAAPG
                     FKSKINLGSNFGGIFRVNFNKIIGIQTEVSFAQKGQRWFKQEDSAKYYNRLVNNYIEI
                     PVLAVVRFGSEKVKFVGYLGTYLAYWSGAYTQNSIQLDKMTDKAENANYVFTKNDRRF
                     DMGLSTGIGVDFRVG"
     assembly_gap    2539..2638
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2639..2829
                     /locus_tag="PLS16_08640"
     CDS             <2639..2829
                     /locus_tag="PLS16_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61187.1"
                     /translation="RRFDMGLSTGIGVDFRVGKGWFELMARHNLGLLNRYKDSPLKSY
                     NCNFNISIAYLFDFTKHE"
     gene            2822..3649
                     /gene="prmA"
                     /locus_tag="PLS16_08645"
     CDS             2822..3649
                     /gene="prmA"
                     /locus_tag="PLS16_08645"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNJ61188.1"
                     /translation="MNNFYIFSLAYPETLDKETFISLVSDFDIEGLEEKSDSVEVYIS
                     LSEKEQYEIFLKDLSETIQLEYTIGELENKNWNAEWESNFEPVLINDKIGIRAIFHEP
                     LQNVDREIIIQPQMSFGTGHHATTAQVMMGMQQLGFSNKKVLDLGSGTAVLAIYAEIL
                     GASEIDALDNDEWCFNNAKENIVLNQCNLIKPLLGSIEVVMTNTYDIILANIHKNFHL
                     EHLTQYALMMKNGAYILLSGFYASDSKEILDKALECNLIANYSSVRDNWAMIILQKK"
     gene            3656..8179
                     /locus_tag="PLS16_08650"
     CDS             3656..8179
                     /locus_tag="PLS16_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61189.1"
                     /translation="MRKTALFILFVFSVFLMNAQALKEFPNDDNEFIKTYTSFLKTCT
                     RLDCIETNTWFEKSFISSKSNAYLPIIKTISNKLLARKAPVYPTFFNFTNLLATIDTT
                     KVTPENVAKNLEALNVVLDQTKVGNIKLFNQFNEYLLDLFRYNNIYLSKSKTWKSTND
                     FQTLIEQNQPVYVFKNTDFIGTTDNDTIVLKNIDAKYFPLSNQLIGTTGKIFLTRAGF
                     DAVNNFVEFKDFDIDLQKSEINIEHTKFSFKPYLEEILDGTYSDRLLLTKGQAKNYPK
                     FVSNRDDIEFKTNGKEVKLVGAFMLEGTDTYCIGTDAKNAQLTIFDKNNHPKIKVQAK
                     KFNVKNFEQIKVEDALMEVELDSSTIVHPYVNFSYDSKTKTVKAYRDLSKPLAKQPFL
                     SDYHQMFIYADEFKWNMDSSQILLGMIGISDLKPAIFESYNYYYPGLENRYKAGNEAG
                     PLLKIYRYYESTLDRNIDAQYIAGDINPTAPFLQTEQVFYKLVEDGYIGYNPSTRTIY
                     VKDKLLNQALSSKKQIDYDNIKFASFKKITNAKMDVKTNELEIYGVEEIKVSNKAQVK
                     IIPGSDTIKVGKNRNMKFGGKVIAGKVDFVGTPFDFDYDSYSLRMDKIDSLVLFIPEG
                     DGKPNENGEVKLVPSQTPIENISGILFIAEADDKAGTRDNLRYPYFSSRDTANTFYDK
                     GKNGKEYDRDKFKFEMEPFQLDSLTIVATENFNVDGVLKTGGIFEDIKTNVSFQEDKS
                     LGTKIDASEKGLKLYGREGKYFNALKLSKDGLGGKGDFEFGSARLFADTAMFFLDSVY
                     AEIDSMHITESSKYKFPDVSSGNVLMNWSIPSDSVVITPTGENKKFTMYNNSVDLSGK
                     MILQKDKLKGVGTLEWTRTKLKSDKIDFSNRSFDVKNGQLRIAADDGSALIKSEDVDA
                     DFNLDTKIANITLNQNDTLPLTTFKYNTNPKYLTFDFDKNTVQLKSYTPSATFFLEST
                     QLTKEYLRFETNKADLNLNDNTIYIGGINELKLADSKVLPSNNEIYIEADGSIRLLKN
                     SVVIFNANKQYHKITNAEIEVLSKNKFQGSGIYTFKTVDDIIQQIPIAKIDVDNSVKK
                     EIVKEEGKSKTKIKVSDEDKIYTYARTEILEEDKIKLNKNIFFKGNMSFDSKHKELDL
                     EGYAKIVLKVVETDWIPFSQKLDPENPEVSMDSILAKATNLVNGVLYDRSTGELYTSV
                     LQEKRASTDPMLFSVKGNMSYDNANSNTIVFGNSNAFKKSIIPGASAMKFDEITKKVD
                     ATGIVNLGLNMGVTNAIAVGSFEYNNDQKMMLKADLAIKTIVSNTILTNVINAFVGSD
                     SLFVYSNYRKKDTLQRTLAYLTNDTIEGKYLLGSIYMKDSLFKPSSMPYDIVLNGTNF
                     LWDNEEASFKSISPINLPYFGNNIVKRPYTAYVEMAYGGTTDFINILLISKNNDWLYL
                     KFKNRQIGIASSVPGIFEYLVGLPESERETRDNKSKMIILDLLPASLSLKIDFEQRME
                     DFKERYKTELGIK"
     gene            8186..8836
                     /locus_tag="PLS16_08655"
     CDS             8186..8836
                     /locus_tag="PLS16_08655"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNJ61190.1"
                     /translation="MISVWKKILSFFWEFKLEAIYSEYSGNLEVSYFQNNYKLSTQNA
                     TYSYGKLYSSFKTAFEAIDIFNREIQNVLILGIGLGSVIEQLNLHKTIQQIDAVDIDA
                     QIIYLCKKYLNTNYNINYIQSDANDFIQNCKHQYDLILVDIFIDDQTPNKFLTENFLH
                     QIKLICSKSCIVLFSKLNVSELNRRENEQFGNYFSAIFPNSFSITTDGNLVFTSID"
     gene            complement(8861..>10340)
                     /locus_tag="PLS16_08660"
     CDS             complement(8861..>10340)
                     /locus_tag="PLS16_08660"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNJ61191.1"
                     /translation="GIATIQLPKDVVYNGALSIIPANAPINYNSTNNTLTWQMGDIPE
                     APLNSILAKMEYTITIPDCENITLNSNCTIDPIAFDGFLYGTNPLTNKPIDTRFTIGY
                     NESTVGCILTKDPIKGPLTYVIDLKNCNEPDIQLSSTKQCTGGNAVINFINPKIASNY
                     VYEWQKLENGTWIDIGNASSNQLNLNNLTSTNNGQYRISLKRYNPENTSGSYCDFSSS
                     SVNINVLSPPLVNINVPDNGILCLGESFNLSTNINDGNWVIDNDQIISINSNGQVIGK
                     SVGVAQITYTVPENETTCQASASSQIMVGSCTVLPIELLSFNAICLENRTITLNWSTA
                     SETNSAYFTLEKSSDAIDFYSIGTISAAGKSSSELQYQYIDKEINGDNYYRLKLTNLD
                     GSESVYNIINIKCNPTQNQINAYYTRNNQIEIEIISNNTTNYIFNLFQANGKQLYNGG
                     IVTNANKTTFKITPEEHLVQGVYLLQIINNDQIETKKIWIAG"
BASE COUNT         3634 a         1303 c         1672 g         3631 t
ORIGIN      
        1 tacttattta tttaaattta gtaattgtgt ttgtcaagtt gttttgtgtt aataatatta
       61 atatatatgc ttttatgcat aattttttcc agctaataat tcttctgcca aatgatagtt
      121 ttctatttgg tctgggtgaa aatctttctg aaaatattga ctaaattgag cagataatgt
      181 gccacctaca ttttcgttga attttttaat gaatgataga atataacctg gtatatcttt
      241 tgcttttaca tcttcatctt gagcagtaag cattgcagtt cctagtaata taaatgacca
      301 taaaccccaa gtggcaattg ccattcctcc gattcttagc caataattat catctaccgc
      361 tttcaaaaca tcaaagggaa ttgctttgtg ctctatttct tcagcagcat gccacataaa
      421 aagttctttc atttctgcag gcattttttt tgtaacatct ttatctaata agccactttc
      481 tgccaacatt gctgtaaaat gttctaagcc aacagtaata ctcaagcaaa ttttatcgcc
      541 caatctattt ttctttagtg tttttcgtgc aaaactttca atgccattcc aagtaatatt
      601 gcgatagatg tttaagaatt tatcaggtct caaaccatgt tcttcaatta catcccaaaa
      661 tttttgatgt tgtgcaaagt gtgtaccttc ttgtccaata aaattatcta cacgtttttt
      721 tagttcggga tcgtttttat aaacatcagc aaaagctttt acacttcgaa taaaaaattt
      781 ctcgccatca ggaaatacca aatgctgtgc attcataaaa tgtgttgcaa taggattgtc
      841 attataccaa tgtttcggaa atggtttctc gaaagtgaaa tttacttttc taatttttgc
      901 ctctggcttt tttttaattt ctgccatata atttatctaa taccaatcaa aggtatttaa
      961 tagatatcta tttagctata aacaaaagat aaaaacagct tttacagatt aaagcggtgt
     1021 ccacgtaaaa attcttgaac agtcatcttt tttttgcctt ctaattgtaa ttctaaaata
     1081 tctataaaag tatcggcagc tctgaatgat aaaaaggttt ttccatcagt ataaaaattt
     1141 ccaatgcttg tatcaataag atttcgttct tcattgataa tagaactgta gatttttaat
     1201 gttttattat ctaaataagt aaatgcagca ggataaggag ataagccacg aataagattt
     1261 tgtacatttt cagcagaatt attccaatta atcaagcaag tttctttaaa tatttttgga
     1321 gcatgttgaa gaattagatt tgaagattgg agattggata tttgagcata gctttgaact
     1381 tcatttttct caactgcttg tactgttttt aaaacaagtt cagcaccaat ttgcattagt
     1441 ttgtcgtgta cacttcccac attatcttgt tctgtaattt gtattttatc ttgaaaaata
     1501 atatttccag tatcaatttc atgttgtaag aaaaatgttg taacaccagt ttctttttcg
     1561 ccattgataa gcgcccaatt gattggagct gctccacgat attgaggtag gagtgaagcg
     1621 tgtaaattaa atgtgccaaa ttgtggcata tcccaaacaa tttctggcaa cattctaaat
     1681 gccacaacta caaataaatt aggttttaag gcttttaatt gctcaataaa ttcaggtgat
     1741 tttagttttt cgggttgtag tacttttaat tgatttgctt cagcatattg tttgactgct
     1801 gaagtttgta atttatttcc acgaccagca ggtttatcag ttgcggttac aacacctaca
     1861 atattgtaat tgttttttac caaaatgtct aaagaagcca ctgcaaattc aggtgttccc
     1921 ataaaaataa ttcttaaatc ttccttctct accatatatt agttatttca gctattaaaa
     1981 atcactatat ttgagcataa aattaagcga tttaatgaaa aagtatgttc ttgtagtgtt
     2041 attattcttt tgtttccatg cagtttttgc agaattggaa tttaatgttg gaggaaaagt
     2101 tggggtaaat ttgtcacata tccgaaagat ggatgcagca ccaggtttta aaagtaagat
     2161 aaatttaggt tctaattttg gtggaatttt tagagtaaac tttaataaaa ttatcggcat
     2221 acaaacagaa gtttcgtttg ctcaaaaagg gcaacgttgg tttaagcaag aggattctgc
     2281 aaaatattat aatcgcttag tgaataatta tatagaaatt ccagttttgg ctgtagttag
     2341 atttggttca gaaaaggtta agtttgtagg atatttaggt acttacttag cctattggag
     2401 tggtgcttat actcaaaata gcattcagct ggataagatg actgataaag cagaaaatgc
     2461 taattatgtt tttacaaaaa atgatagaag atttgatatg ggtttaagta ctggaattgg
     2521 tgtggatttt cgagttggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat
     2641 agaagatttg atatgggttt aagtactgga attggtgtgg attttcgagt tggcaagggt
     2701 tggtttgaat taatggctag acataattta ggcttattga atcgatataa agatagtcct
     2761 ctaaaaagct ataattgcaa ctttaatatt tctattgctt acttatttga ttttacaaaa
     2821 catgaataat ttttatatct tttcattagc ttatcctgaa acattggata aagaaacttt
     2881 tatcagttta gtttccgatt ttgatattga agggcttgaa gaaaagtctg attctgttga
     2941 agtgtatatt agtctgtcag aaaaagagca gtatgaaatt tttttgaaag atttatcaga
     3001 aacgatacaa ttggaatata caattggaga attagaaaat aagaattgga atgcagaatg
     3061 ggaaagtaat tttgagccag ttttaataaa tgacaaaatt gggattcgag caatttttca
     3121 tgaacctttg caaaatgtag atcgagaaat tattattcaa cctcaaatgt cgtttggaac
     3181 tggacaccat gcaacaactg ctcaggtaat gatgggtatg caacaacttg gtttttcaaa
     3241 taaaaaagtt ttggatttag gaagtggaac agctgttttg gcaatttatg ctgaaatatt
     3301 aggagcttcg gagattgacg cattagataa cgatgaatgg tgctttaata atgccaaaga
     3361 aaatatagta ttgaatcaat gtaatttgat aaagccgttg ttaggtagta ttgaagtggt
     3421 aatgaccaat acttatgata ttattttagc aaatattcat aagaattttc acttagaaca
     3481 cttaacgcaa tatgctttaa tgatgaaaaa tggtgcttat attttattaa gtggatttta
     3541 tgcttctgat tcaaaagaga ttttagataa ggctttggaa tgcaatttga tagcaaacta
     3601 tagttcggtt agagataatt gggcaatgat aattttacaa aaaaaataaa tatttatgag
     3661 aaaaacagca ctatttatac tatttgtttt ttctgttttt ttaatgaatg cccaagcatt
     3721 aaaagaattt ccaaatgatg ataatgaatt tataaaaacc tataccagct ttttaaaaac
     3781 ttgtactcgt ttggattgta ttgaaacaaa tacttggttt gaaaaatctt ttataagcag
     3841 taaatcgaat gcatatttgc caattataaa aacgatttca aataaattat tagccagaaa
     3901 agcacctgtt tatcctactt ttttcaattt tacaaattta ttagcaacta ttgatacgac
     3961 aaaagttaca cctgaaaatg ttgctaagaa tttagaagca ttgaatgttg ttttagatca
     4021 aactaaagtt ggaaatataa aattatttaa tcagtttaat gaatatttat tagacttgtt
     4081 cagatacaat aatatttatt tatcaaaatc aaaaacatgg aaatcaacga atgattttca
     4141 aacattgata gagcaaaatc aaccagttta tgtatttaaa aataccgatt ttattggtac
     4201 gactgataat gatacgattg ttttgaaaaa tattgatgca aagtattttc cacttagcaa
     4261 tcaattgata ggtactactg gaaaaatatt tttaactaga gcaggttttg atgcagtaaa
     4321 taattttgta gaatttaaag attttgatat tgatttacaa aagtctgaaa ttaatataga
     4381 acatactaaa tttagtttca aaccctattt ggaagagata ctagatggta catatagcga
     4441 tagattatta ttgactaaag gtcaggcaaa aaattatcca aaatttgttt cgaatagaga
     4501 tgatatagaa tttaaaacaa atggaaagga ggtgaaatta gttggagctt ttatgttgga
     4561 aggaacagat acgtattgta taggaacaga tgctaaaaat gctcaattga ctatttttga
     4621 caagaataat catccgaaaa ttaaagttca agcaaagaaa tttaatgtta aaaattttga
     4681 acaaataaaa gtagaagatg cattaatgga agttgagtta gatagtagta caattgtaca
     4741 cccttatgtc aatttttctt atgattctaa aactaaaaca gttaaagcct atagagattt
     4801 atcaaaaccc ttagccaaac aaccattttt aagtgattat catcagatgt ttatttatgc
     4861 tgatgaattt aaatggaata tggatagctc acagatttta ttaggaatga ttggtatttc
     4921 agatttaaaa cctgccattt ttgaatctta taattattac tatccaggtt tggaaaatag
     4981 atacaaagca ggaaatgagg ctggtccact tttgaaaatt tatagatatt atgaaagtac
     5041 tttagataga aatattgatg ctcaatatat tgctggcgat atcaacccaa cagcaccatt
     5101 tttacaaaca gagcaagtat tctacaaatt ggttgaagat ggttatattg gttataatcc
     5161 ttcgacaaga actatttatg taaaagataa gcttctaaat caagccttgt cttcaaaaaa
     5221 gcaaattgac tacgataata tcaaatttgc ttcttttaaa aaaattacca atgcaaaaat
     5281 ggatgttaaa accaatgaat tagaaattta tggtgtagaa gaaattaaag ttagtaataa
     5341 agcacaagta aaaataattc cagggtctga tactataaaa gtgggaaaaa atcgaaacat
     5401 gaagtttggc gggaaggtaa ttgcagggaa agtagatttt gtaggcacgc cttttgattt
     5461 tgattacgat agctattcat taagaatgga taaaatagac tcattagttt tgtttattcc
     5521 agagggcgat gggaagccaa atgaaaatgg cgaagtaaag ttagtgcctt ctcaaacacc
     5581 aatcgagaat atttcaggta tattatttat tgccgaagca gatgataaag caggaactcg
     5641 agataattta agatatcctt atttctctag tagggataca gctaatactt tctatgataa
     5701 agggaaaaat gggaaagaat atgatagaga taagttcaaa tttgaaatgg aaccctttca
     5761 actagattct ttaactatcg tagcaactga aaatttcaat gttgatggtg tattaaaaac
     5821 aggaggtata tttgaagata ttaaaactaa tgtgtccttt caagaagata aatcattggg
     5881 tacaaaaatc gatgcatctg agaaaggatt gaaattatat ggaagagaag gtaagtattt
     5941 taatgcattg aaattatcta aagatggttt aggtggaaag ggtgattttg aatttggaag
     6001 tgcaagatta ttcgcagata ctgcaatgtt tttcttagat agcgtatatg cagaaattga
     6061 tagtatgcat attacagaaa gtagtaaata taaatttcca gatgtgtctt cggggaatgt
     6121 gcttatgaat tggtcaattc cttctgattc cgttgtaata acaccaacag gtgaaaataa
     6181 aaagtttact atgtataata actcggtaga tttatcgggt aaaatgattt tgcaaaaaga
     6241 taaattgaaa ggtgttggaa cattagaatg gactagaaca aaattaaaat cagataagat
     6301 tgatttctct aatagaagtt ttgatgtgaa aaatggacaa ttaagaattg ctgctgatga
     6361 tggttctgct ttaataaaat cagaagatgt cgatgctgat tttaatttag atactaaaat
     6421 tgctaatatt acattgaacc aaaatgatac cttgccactt actactttta aatataatac
     6481 taatccaaaa tacctaactt ttgattttga taaaaataca gtacagttaa agtcatatac
     6541 tccaagtgca acatttttct tggaatcaac acaacttact aaagaatatt tacgatttga
     6601 aaccaataaa gctgacttaa acttaaatga taatacaatt tatattggtg gtattaatga
     6661 attaaaattg gctgattcaa aagtattacc ttctaataat gaaatctata ttgaagctga
     6721 tggtagtatt agattactta aaaattccgt ggtgatcttt aatgcaaata agcaatatca
     6781 taaaataacc aatgcagaaa ttgaagtttt atccaaaaat aaatttcaag gcagtgggat
     6841 ttacactttt aaaactgtag atgatattat tcaacaaatt cctattgcta aaattgatgt
     6901 tgataattct gtaaaaaaag agattgttaa agaagaagga aaaagtaaaa ctaaaattaa
     6961 agtttcagat gaagataaaa tttatactta tgcaagaact gaaattttag aagaagataa
     7021 aatcaaattg aataagaata tcttctttaa aggtaatatg tcttttgatt caaagcacaa
     7081 agaattagat ttagaaggtt atgctaaaat agttttaaaa gttgttgaaa cagattggat
     7141 tccattctct caaaagttag accctgaaaa tccagaagtt agcatggata gtattttggc
     7201 taaggctaca aatttagtga atggcgtgct atatgataga agcactggcg aattatacac
     7261 ttctgtacta caagaaaaac gtgcatcaac tgacccaatg ttattttctg taaaaggaaa
     7321 catgagttat gataatgcaa attcaaatac tatagttttt ggaaatagca atgcctttaa
     7381 aaagtcgata ataccaggag cttcagctat gaagtttgat gaaattacaa agaaagttga
     7441 tgcaactgga atagtaaatc ttggattaaa tatgggtgtt acaaatgcta ttgctgttgg
     7501 tagtttcgaa tataataacg accaaaaaat gatgttaaaa gcagatttgg ctattaaaac
     7561 aatagtatcc aatactattt tgacaaatgt tattaatgca tttgttggtt cagattctct
     7621 atttgtttat tctaattata gaaagaagga taccttacaa agaacattag catatcttac
     7681 caatgataca attgaaggta aatatttatt aggttctatt tatatgaaag attctttatt
     7741 taaaccatct agcatgccgt atgatattgt tttaaatggt acaaatttcc tatgggataa
     7801 tgaagaagct tcattcaaat ctatctcacc aattaatcta ccatactttg gaaataatat
     7861 tgtgaaaaga ccatatactg cttatgtgga aatggcttat ggaggaacta ctgattttat
     7921 aaatatactt ttaataagca aaaacaatga ctggttatac cttaagttta aaaatcgaca
     7981 aattggtatt gcatcttctg ttccaggtat atttgaatat ttggtaggtt tacctgagtc
     8041 agaaagagaa actagagata ataaatctaa aatgataatt cttgatttat tgcctgcaag
     8101 tctttcactt aaaattgatt ttgaacaaag aatggaagat tttaaagaac gttataaaac
     8161 ggaattaggt ataaaataac tgcctatgat ttctgtttgg aaaaaaatat tgagtttttt
     8221 ttgggaattc aaattggagg ccatttattc tgaatatagt ggaaatttag aggtttcata
     8281 ttttcaaaat aattataagt taagtacaca aaatgctact tattcttatg gaaaattata
     8341 ttcttccttt aaaacagcat ttgaagcaat agatattttt aatagagaaa ttcaaaatgt
     8401 cttaatctta ggaattggct taggtagcgt tattgagcaa cttaatttac ataaaacaat
     8461 tcaacaaatt gatgcagtag atattgatgc tcaaattatt tatctgtgca aaaagtactt
     8521 aaatacgaat tataatatta attatattca aagcgatgcc aatgatttta tccaaaattg
     8581 taaacaccaa tacgatttga ttttagttga tattttcatt gatgatcaaa ctcctaataa
     8641 atttctaact gaaaatttct tacatcaaat aaaattgatt tgtagtaaaa gttgtatagt
     8701 cttatttagt aaattgaatg tatctgaatt aaatcgaagg gaaaatgaac agtttggaaa
     8761 ttatttcagt gcaatatttc ctaattcttt ttcaataact actgatggaa atttagtttt
     8821 tacttccatt gattgattct agtattttaa attcaatttt ttaacctgca atccaaattt
     8881 ttttagtttc aatctgatca ttattgatta tttgtaatag atacactcct tgaactaaat
     8941 gttcctcagg agttatttta aaggtagttt tgtttgcatt ggtaacaata ccaccattgt
     9001 aaagttgttt tccatttgct tgaaataaat tgaatatata atttgtagta ttattagata
     9061 tgatttctat ttcaatttga ttatttcttg tataataagc attaatctga ttctgtgttg
     9121 ggttacattt tatattaatg atgttatata cactttcaga accatctaaa tttgtaagtt
     9181 ttaatctgta atagttgtct ccatttattt ctttgtctat atattgatat tgaagttcac
     9241 ttgaagattt tccagcagct gaaattgtac ctatcgaata aaaatcaata gcatctgaag
     9301 atttctctaa tgtaaaatag gcactattgg tttctgatgc tgtactccaa ttaagtgtaa
     9361 ttgttctgtt ttctaaacat atagcattaa aggaaagcaa ttcaattgga agtacagtac
     9421 agctacccac cattatttga cttgatgctg atgcttgaca agtagtttca ttttctggaa
     9481 cagtataagt tatttgagct acacctaccg attttccgat aacttgtcca ttactattaa
     9541 tagatattat ttggtcatta tcaattaccc aatttccatc attaatattt gtagataaat
     9601 taaaagattc accaaggcat aaaattccat tatcaggtac attaatattt actaaaggcg
     9661 gtgataaaac gtttatattt acactagatg aagaaaaatc acagtaagaa ccagatgtat
     9721 tctccggatt atatcgttta agtgaaattc tatattgtcc attgtttgta gatgttaaat
     9781 tatttaagtt tagttgattg cttgatgcat tgcctatatc tatccaagtt ccattttcta
     9841 atttttgcca ttcatataca tagttacttg caatttttgg attaataaaa ttaataactg
     9901 catttccacc agtacattgc tttgttgaac ttagttgaat atcaggttcg ttacagtttt
     9961 ttaagtcaat aacataagta agaggacctt ttattgggtc tttagttaat atacaaccga
    10021 ccgttgattc attataacca attgtgaacc ttgtatcgat aggtttatta gttagcggat
    10081 tcgttccata aaggaatcca tcaaaagcta ttggatcaat agtgcaatta gaatttaaag
    10141 tgatgttttc acaatcggga atcgtaatgg tatattccat ctttgctaaa attgaattca
    10201 aaggagcttc tggaatatct cccatttgcc aagttaatgt attatttgtg gagttatagt
    10261 ttattggtgc attagctggt attatactta aagctccgtt atagacgaca tcttttggaa
    10321 gttgaatagt agcaatacca
//
LOCUS       DAOQCW010000093        10297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_2451_length_10297_cov_5.110428, whole genome shotgun sequence.
ACCESSION   DAOQCW010000093 DAOQCW010000000
VERSION     DAOQCW010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10297
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2451_length_10297_cov_5.110428"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PLS16_08665"
     CDS             <1..517
                     /locus_tag="PLS16_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNJ61192.1"
                     /translation="AFVPNSVGATKGSGSATVEASDIAPAASKVTVKDVKREGGNVAP
                     LPEAELVVSAGRGMKGPENWGIIEDLANVLGATTACSRPVADVGWRPHHEHVGQTGVA
                     IRPNLYIAIGISGAIQHLAGVNQSKVIVVINKDPEAPFFKAADYGIIGDAFEVVPKLT
                     EAIKAFKASQG"
     gene            685..1188
                     /locus_tag="PLS16_08670"
     CDS             685..1188
                     /locus_tag="PLS16_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HNJ61193.1"
                     /translation="MKYNIIYNENKAHEIELKDNKIIIDGQNADIDLVHIYKEKYHII
                     DNNESFNIEVVRADFQNKTFEIKVNDTIYPIQLEDELDMLLEKMGMSAVGSNTMDNVK
                     APMPGLVLRITVDVGQEVNKGDNLIVLEAMKMENIIKATGTGKVKNILVTEKQAVEKN
                     QLLIEME"
     gene            1197..1847
                     /locus_tag="PLS16_08675"
     CDS             1197..1847
                     /locus_tag="PLS16_08675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HNJ61194.1"
                     /translation="MEIIPENIENYISKYASGETNVLAELERYTHANVLLPNMLSGKA
                     QGIFLQLFSAAVQPKYILEIGTFTGYSAICLAKGLQNGGQLFTIDVNEELESVAKKFW
                     NKAGLDDKITIIIGDAQIEIPKLDYQFDIIFIDADKKNYSLYYDLVIDKIKPNGFILA
                     DNVLWKGKIINEQKDKDTEALHLFNEKIAQDNRVENVILSIRDGINIIRKKTTNEN"
     gene            1837..3330
                     /locus_tag="PLS16_08680"
     CDS             1837..3330
                     /locus_tag="PLS16_08680"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNJ61195.1"
                     /translation="MRTKILIALFCLFNLTTLGQAQEIKSPEIIAYIEKYKDIAILEM
                     KRAKIPASITIAQGILESGFGKSVLALNTNNHFGIKCHQNWTGKTFKYNDDAPNECFR
                     VYDDPIQSYKDHSDFLVNRSRYANLFTLDIKDYKAWAYGLKEAGYATNPKYPELLIKY
                     IETYSLDKLDNGTFSKEELLAENYKKDEKNINKVESVNTKLHQIDNKEQDVIKSKTLK
                     KSVVLYNKLKAVKVFSHDDLALVAQSAKISLKNLLQYNDLDDASIRLNSQYIYLEPKR
                     TKAKHKTHLIKKDDTMWKIAQRHAIQLDALLKLNKMSKGEEPKVGELINLKKPIKVKP
                     QLRTTPIQNKTETIPNKQETIKTTSIEVKTTVSTSKDTIYKNIPKQNTSVDEEKVLSW
                     EEGTKLVENNPVKPFPDTKIEEKIEPIKNTATNLETIEVSEKKTEVSTPIIELKKVDP
                     NASVHLVEKGDTLYNISKRYGITVAQLIEWNNIQDNVVKLGTYLKVK"
     gene            complement(3409..4809)
                     /locus_tag="PLS16_08685"
     CDS             complement(3409..4809)
                     /locus_tag="PLS16_08685"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HNJ61196.1"
                     /translation="MVAELSTTINAAELVRKHRQFFNSGKTQSLEFRLEQLNKLRSLI
                     KANESKIKEALKKDLNKSEFEAYGTEITFVLKDLDHVIASLKKWMKPERVKTPLFHQL
                     GSSWIQSDPLGVVYVIAPWNYPFQLLIAPVIGAMAAGNTVIMKPSRMSANTCQLIDEM
                     ISKNFPADYLKVITNFQTTQELLEEKFDYIFFTGGKEVGTEIYQKAAKNLTPVTLELG
                     GKSPCFVDETMNMNWAMKRLIWGKFTNAGQTCVAPDYFLVDNKIKAKFISEAIKTIKE
                     FYGEDPKQSPDFGRIITEKHFQRVSGLFKNAQVIHGGDTDASQKYIAPTILDNVKLDD
                     AVMQEEIFGPLMPIIGYDSLDEAIKIVKSGGKPLALYVYSEDKAYQQYVLNETSSGNG
                     SINECLMHVGQFNLPFGGVGDSGIGQYHGKLSFDIFSHRKGVLKKSTLTDLALRFPPY
                     NSNKVNMLNKLMDWLM"
     gene            complement(5008..5712)
                     /locus_tag="PLS16_08690"
     CDS             complement(5008..5712)
                     /locus_tag="PLS16_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016268617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ61197.1"
                     /translation="MNKKVLLVEDDPNLGALLQEYLKHKNFDVDLKRDGVEGLFAAKK
                     NKYDMLLLDVMMPKKDGFEVAEEIRKDNQEVPIIFLTAKSMKEDKIKGLTIGADDYIT
                     KPFSMEILELKMNAILRRTDKTDVKVALDTYKVGSSVLDYKNHKLNINGKETKLTTKE
                     TELLRLFFERKNELLEREFALKHVWQDDSYFTARSMDVFISKIRKYLKDDETLQILNI
                     HGQGYKLIDNSESTEL"
     gene            complement(5715..7172)
                     /locus_tag="PLS16_08695"
     CDS             complement(5715..7172)
                     /locus_tag="PLS16_08695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNJ61198.1"
                     /translation="MNRRIIILTTVLLLLGLLGIATVQVFWLQKAIQYSEQNFDKNIF
                     QALYDVSTEIDNLKVSPLISEAMGAKDINSKIKGDIIMRTIDEEGKPTSLFYNDSMTA
                     EQYAAIREQIEESMPLQIDNLQQLMAEQMVSLTPIQELLDTTKLASLISKALASKGIE
                     ICFRYGITEFAPNNFVLISKNTPFGELYKTKYFIELFPRSIIDQKKTLRLFFPHKSQY
                     IYASIWQIIVSSVFFFLLTVAAFILAFKIIFQQKKLSDMKNDFISNMTHELKTPIATI
                     SIASEMLKDNTISADSNNRKKYANIIFTENKRLANHVEQVLQIAKLENGELELNKADR
                     HLHLIIESVAERFLLQLEELGGEIILDLEAKNDIFKVDELHFSNMINNLIDNAIKYND
                     KQPLIKINTYNIITGIVIEVSDNGIGLSKENQAKIFDKFYRVSKGNVHDVKGFGLGLS
                     YVMSIVKGHFGEISVESKLKEGTKFKIQIPNNQTE"
     gene            complement(7290..9125)
                     /locus_tag="PLS16_08700"
     CDS             complement(7290..9125)
                     /locus_tag="PLS16_08700"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HNJ61199.1"
                     /translation="MFLIGLGKADITAFERGSSMLGYGMYSHIALDVETPLFARAYLI
                     QDIDKQNKIAIVNCEVCFITPLLKQSVVDYIAKHFPDLGLTDDNLMLSATHTHCSPAG
                     FSSHATYNISVPGFVQHILDKLVKGIVEAIVEADKVKVKANITIGKSNFANDIPVSFN
                     RTVNSYNQNPEVKKYSFSERHLAVDRAMTMLNFVSEEGALLGSINWFAVHTSSLPNNF
                     LKLCSDNKGYAADYLERDLQKKNKNYIGAFAQGACGDVSARVRYNPNLPFQRGKYEGF
                     YSDDLKSSKFCGQLQYEKAKEIVENNQTQINNNTIDFVQQYIDFSKIDILPEFNNGKT
                     GGVTSPAVMGVAFLEGSKMDGPGMHPFIGNFAKTLSNIVRNKEINKAKNKSDIESEKT
                     HRRYTAQGNKHLAFETGEKRLFSFTDIKNFFIPGAVDPMIHYLKHFASLGLLDEHPWS
                     PQILPLQIFKLGTIAIVGFPFEITTVASWRLKKTLEDILFKQGIEHIILSPYANAYNG
                     YITTLEEYQVQDYEGGHCVFGQWSLNALQQVSSNLATQMGKEKADRDIPFLREAPISD
                     KYLERFAYTKSFYYQKIEKKLAKKENKILLSSANYDKKIDAQIGQ"
     gene            9191..10237
                     /locus_tag="PLS16_08705"
     CDS             9191..10237
                     /locus_tag="PLS16_08705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ61200.1"
                     /translation="MGINKEISVSVLMTAYNAENYVKEAIQSILEQTYSDFEFVIIND
                     GSTDKTLEIIQSFDDKRIRIINEGKFGYYKAKRRLIEEAKGKYIAIMDADDIADKNRF
                     EAEVDFLDKHPDFGLVGTNATWIDKDNKPFGKGFNFSYSPEELKCRLLFHNCFVHSSV
                     LIRKSILDKYQLNYREKASEDLDLWVQISRYIEVKNIPKKLIKYRIHSNSIVHSNWYK
                     KDNGLKQITFEQLDYYFPEILSVEEKNAIFGLVNFDVENDNKVMSIILKSIQKLLQSN
                     QKNEHFNDIFFRKVIQERWFKKIIRLKEYSSEIWKTNKECKEILDIPLTPKQHLFEII
                     LILNIILKKRIIKV"
BASE COUNT         3701 a         1585 c         1499 g         3512 t
ORIGIN      
        1 tgcatttgtt ccaaattcag ttggtgctac aaaaggaagt ggtagtgcaa ctgttgaggc
       61 ttctgatatt gctccagctg catcaaaagt tactgtaaaa gatgttaaga gagaaggcgg
      121 aaatgttgct ccacttcctg aagctgaatt agtagtaagt gctggtcgtg gtatgaaagg
      181 acccgaaaac tggggtatta ttgaagattt agcaaatgta ttaggtgcga ctacagcatg
      241 ttctcgtcca gttgctgatg tgggttggag acctcatcac gaacacgttg gacaaacagg
      301 tgttgctata cgtccaaatt tatatattgc aataggtatt tctggtgcaa tacaacattt
      361 agctggtgtc aaccaatcaa aagtaatcgt tgttatcaat aaagatcctg aagctccttt
      421 ctttaaggct gctgactatg gaattattgg agatgcattt gaagttgttc caaaattaac
      481 agaagctatc aaagctttta aagctagcca aggctaatat ttgatagttt taaatactaa
      541 aatgccgact tttgtcggcg tttttttttg tttattccat ccaaaatctc ttttatatta
      601 acaatagtta ttatcaacaa gtatttaaaa ctaaaaggca aaaattttcg tttttatttc
      661 taatattgta aaaattgcag tttaatgaaa tacaatatca tttataatga gaataaagct
      721 catgaaattg agttgaagga taataaaatc atcatagatg gacaaaatgc cgatattgat
      781 ttagtacaca tttacaaaga aaaatatcat attatagata ataatgaatc atttaatatt
      841 gaagtggttc gggctgattt tcaaaataaa acttttgaaa taaaagtaaa cgatactata
      901 tatcctattc aattagaaga tgaattagat atgctattgg aaaaaatggg tatgagtgcc
      961 gtaggcagta ataccatgga taatgttaaa gcaccaatgc ctggtttggt tctaagaatt
     1021 accgttgacg ttggtcaaga agtcaataag ggcgataatc ttatcgtatt agaagctatg
     1081 aaaatggaaa atattatcaa agcgactggg accggtaaag tgaaaaatat tttagttaca
     1141 gaaaaacaag cggtggagaa aaatcaattg cttattgaaa tggaataaaa ttttatatgg
     1201 aaatcatacc tgaaaatata gaaaactata tttccaaata tgcatctgga gaaacaaacg
     1261 ttttagctga attagaaaga tatactcatg cgaatgtatt attaccaaat atgctttctg
     1321 gtaaagcaca aggtattttc ttacaattat tttcagcagc agtgcaacca aaatatattt
     1381 tagaaatcgg aacatttacg ggttattctg caatctgttt ggcaaaagga ttacaaaatg
     1441 gtggtcaatt atttacgatt gatgtaaatg aagagttgga aagtgtggct aaaaaatttt
     1501 ggaacaaagc aggtttagat gataaaataa ccataataat tggtgatgca cagatagaga
     1561 taccaaaatt agattatcaa ttcgatatta tatttataga tgctgacaaa aaaaattatt
     1621 ctttatacta tgatttagtg attgataaga taaaacctaa cgggtttatt ttagctgaca
     1681 atgtactgtg gaaaggaaaa ataattaacg aacaaaaaga taaggatact gaggcattgc
     1741 atttgttcaa tgaaaaaatt gctcaagata atcgtgtaga aaatgttatt ttatctattc
     1801 gagatggtat taatattata agaaaaaaga caacaaatga gaactaaaat tttaatagca
     1861 ttattttgct tatttaattt aaccactttg ggacaagctc aagaaataaa gagtcctgag
     1921 attattgctt atatcgaaaa atacaaagac attgctattt tagaaatgaa aagagcaaaa
     1981 attccagcaa gtattacgat tgcacaaggc attctcgaat caggttttgg aaaaagtgta
     2041 ttagcattaa acacaaataa ccatttcgga ataaaatgtc atcaaaattg gacaggaaag
     2101 actttcaaat ataatgatga tgctcccaat gaatgtttta gagtgtatga tgatccaatt
     2161 caatcctata aagatcactc agatttctta gtcaatcgta gtcgatatgc gaatctcttt
     2221 acactagata ttaaagatta caaagcgtgg gcttatggtt tgaaagaagc tggctatgcc
     2281 accaatccaa aataccctga gttattaata aaatatattg aaacttatag tttagataaa
     2341 ttagataacg gtactttctc taaagaagaa ttattggcag agaattataa aaaagatgaa
     2401 aaaaatatca ataaagttga aagcgttaat acaaaattac atcaaataga taataaagag
     2461 caagatgtta ttaaatctaa gactttaaaa aagagtgttg ttttatataa taaattaaaa
     2521 gctgtaaaag tatttagtca tgatgattta gcattggttg ctcaatccgc taaaatttca
     2581 ttaaaaaatt tattacaata taatgattta gatgatgctt ctattcgact aaatagtcaa
     2641 tatatttatt tagagccaaa gcgaactaaa gcaaagcaca aaacacatct tatcaaaaaa
     2701 gatgatacca tgtggaaaat tgctcaaaga cacgctatac aattagatgc cttattaaaa
     2761 ttgaataaaa tgtctaaagg cgaagaacca aaagtgggtg aattaatcaa tttaaagaaa
     2821 cccattaaag tgaaacctca actcagaaca acacccattc aaaataaaac agaaactatt
     2881 cctaataaac aagaaactat aaaaacgact tcaattgaag taaaaacaac ggtttctact
     2941 tcaaaagata ctatttataa aaatattcct aaacaaaata cctcagttga tgaagaaaaa
     3001 gttttatctt gggaagaagg cactaaatta gttgaaaata atccagtaaa gccttttcct
     3061 gatacaaaaa ttgaagaaaa aattgaaccc ataaaaaaca ctgctacaaa tctagaaaca
     3121 atagaagttt cagagaaaaa aacagaagtt tctactccaa tcattgaatt aaaaaaagtt
     3181 gacccaaatg cttccgtaca tttagttgaa aaaggagata ctttatacaa tatcagcaaa
     3241 cgatacggta ttactgttgc tcaattaatt gagtggaata atattcaaga taatgtcgtt
     3301 aaattaggca cttatctgaa ggtgaaataa taagaattag actataaaat acaaaaaggc
     3361 ttagatatat ctaagccttt ttactttatt aggataaact gtatagtatt acatcaacca
     3421 atccatcaat ttattcaaca tattaacttt gttagaatta tatggcggga aacgtagtgc
     3481 caaatccgtc aatgtagatt ttttcaaaac gccttttcta tgtgagaaaa tatcaaagct
     3541 taattttcca tgatactgac caattccgct atctcctaca ccaccaaatg gcaaattaaa
     3601 ctgtccaaca tgcattaaac attcattgat agagccattt ccagatgatg tttcattaag
     3661 tacatactgc tgatatgctt tatcttcaga atacacatat aatgccaatg gtttacctcc
     3721 agatttaaca attttaatgg cttcatctaa actatcgtac ccaataattg gcattaatgg
     3781 accaaagatt tcttcttgca ttactgcatc atctaatttc acattatcca aaatagttgg
     3841 agcaatatat ttttgactgg catctgtatc gccaccatga attacttgag catttttaaa
     3901 caatcctgaa actctttgaa agtgtttttc tgtaataatt ctaccgaaat ctgggctttg
     3961 tttagggtct tcgccgtaaa attccttaat ggttttaatg gcttcgctaa taaattttgc
     4021 tttaatttta ttgtccacca aaaaataatc tggagcaaca caagtttgac ctgcattggt
     4081 aaatttaccc caaattaaac gcttcattgc ccaattcata ttcatcgttt catcgacaaa
     4141 acaaggcgat ttgccaccta attctaatgt aactggtgtt aagttttttg cagctttttg
     4201 gtagatttcc gttcccactt ctttaccgcc tgtaaagaaa atatagtcaa atttttcttc
     4261 taataactct tgtgttgttt gaaaattggt aatcactttt aaataatcag caggaaaatt
     4321 tttagaaatc atttcatcta ttaattggca agtatttgca ctcattctag atggtttcat
     4381 aatcactgta ttaccagcag ccattgcacc aattacagga gcaattaata attgaaaagg
     4441 atagttccaa ggtgcaatta cataaaccac accaagtgga tcactctgaa tccagctact
     4501 tcccaattga tgaaaaagag gcgttttaac acgctccggt ttcatccatt ttttcaatga
     4561 tgcaattaca tggtctaagt ctttcaatac aaaagtaatt tcagttccat aagcttcaaa
     4621 ttcagattta tttaaatctt tttttagtgc ttctttgatt ttactttcat ttgctttaat
     4681 taaacttcta agtttattta attgctctaa tctaaattct aagctttgtg ttttgcctga
     4741 attaaaaaac tgacgatgtt ttctaactaa ctcggctgca tttattgttg ttgataattc
     4801 tgctaccata atatctattt taattaaatt aaagtactaa aatactaaaa atctgtggta
     4861 ttttctattt gtaacaaact aaattatctt ttgttgtttt tatactcgga attttataaa
     4921 accacttctc tattaattta agacattaaa taaaaaaatc cgcaatattt ctattgcgga
     4981 acaaattttt taaaaagtaa attaaattta caattctgtt gattcactat tatcaatcag
     5041 cttataacct tgaccgtgaa tgtttaaaat ttgcaatgtt tcatcatctt ttaggtattt
     5101 tctaattttt gagatgaaaa catccatact tctagctgta aaataactat catcttgcca
     5161 aacatgtttt aaagcaaact cacgttctaa caattcattt ttacgttcaa aaaataaacg
     5221 caacaactct gtttctttag tcgtcaattt agtttctttc ccattaatat ttaatttatg
     5281 gtttttgtaa tctaaaacag aactacctac tttgtaagta tctagagcta ctttcacatc
     5341 agttttatct gttcttctta aaatggcatt catctttaat tctaagattt ccattgagaa
     5401 aggtttagtg atataatcat cggcaccaat ggttaaacct ttgattttat cttctttcat
     5461 agactttgct gttaagaaaa taattggtac ttcttgattg tcttttctga tttcctcagc
     5521 gacttcaaag ccatcttttt tgggcatcat aacatctaaa agtaacatat catatttgtt
     5581 ctttttggca gcaaaaagcc cttcaacgcc atctcttttt aaatctacgt caaagttttt
     5641 gtgctttagg tattcttgta gtaaggctcc taagtttgga tcatcttcta ctaataaaac
     5701 ttttttattc ataattactc tgtttgattg tttggtattt gtattttaaa tttagttcct
     5761 tcttttaatt tactttcaac agaaatctct ccaaaatgtc cttttacaat ggacattaca
     5821 taactcaagc ctaaaccaaa acccttcaca tcgtgtacat ttccttttga aactcgataa
     5881 aacttatcga atatctttgc ttgattttct tttgacaatc ctattccatt atcacttact
     5941 tcaataacta tgcccgttat tatattataa gtgttaattt ttattaaagg ttgtttatca
     6001 ttgtatttta ttgcattgtc tatcaagtta ttaatcatat tagaaaaatg caactcatca
     6061 actttaaaaa tatcattttt agcttccaag tccaagataa tctcacctcc taactcttct
     6121 aattgcaata agaaacgctc tgctacagat tcaataatta gatgtaaatg acgatctgct
     6181 ttatttaatt ctaattctcc attttctaat tttgcaattt gtaaaacttg ctccacgtgg
     6241 ttagctaatc ttttattttc agtaaaaata atattggcat atttctttct attattgcta
     6301 tctgccgaaa tggtattatc cttcaacatt tcactagcaa tcgatatagt agcgatgggc
     6361 gtttttaatt catgtgtcat attgctgata aaatcatttt tcatatccga caattttttc
     6421 tgttggaaaa tgattttgaa agctaaaatg aatgcggcca cagttaataa gaaaaagaat
     6481 actgaactta caataatctg ccaaatcgat gcataaatat attggctttt atgtggaaaa
     6541 aacaaacgaa gtgttttctt ttgatcaatg atacttcttg gaaataattc tatgaaatat
     6601 ttagttttat ataattctcc aaaaggtgta tttttagaaa tcaacacaaa gttattcggt
     6661 gcaaactctg taattccgta gcggaaacaa atttcgatac ctttacttgc taatgctttt
     6721 gaaattaatg aagccaattt ggttgtatct aataattctt gaattggtgt caaactaacc
     6781 atttgttccg ccattaattg ttgtaaatta tcaatttgta atggcattga ctcttcaatt
     6841 tgttcacgaa ttgcagcata ttgttcggca gtcatcgagt cattataaaa caaagacgtt
     6901 ggctttcctt cctcatcaat cgttcgcatt ataatatctc ctttaatttt ggagttgatg
     6961 tctttagcac ccattgcctc agaaattaaa ggcgaaactt ttaaattatc aatctctgta
     7021 gaaacatcat ataaggcttg aaaaatattt ttatcaaaat tttgttctga atattgaatc
     7081 gctttttgta gccaaaacac ttgtactgtt gcaattccta acaaacctaa gagcaacaaa
     7141 actgtagtta aaataataat ccgcctattc acgagagcaa atataaaatg aaattcagaa
     7201 caaatagtta atttaactat ttttaaccgc taaaaaaaat attattaaaa attttctttt
     7261 gcttgatttt atctataagc aatttgaaat tattgaccta tttgagcatc tattttctta
     7321 tcgtaattgg ctgagcttaa caagatttta ttttcctttt ttgctaattt tttttcaatt
     7381 ttttgataat agaaactttt agtataggca aaacgttcta aatatttatc agaaattgga
     7441 gcttcacgca agaacggaat atctctatct gctttttctt tacccatttg agtagctaaa
     7501 ttagaactta cttgttgcaa agcattcaaa ctccactgac caaaaacaca gtgtccacct
     7561 tcgtaatctt gcacttgata ctcttctaaa gtagtgatat agccattgta agcatttgca
     7621 taaggcgata aaattatatg ctcaatacct tgtttgaata aaatatcttc tagtgttttt
     7681 ttcaaacgcc aactcgcaac agtagtgatt tcaaaaggaa aaccaacaat tgcaattgta
     7741 cccaatttaa aaatttgtaa aggtaaaatt tgtggcgacc aaggatgttc atctaacaag
     7801 cctaaacttg caaaatgttt taaataatga atcattggat caacagcacc tggtataaaa
     7861 aaatttttaa tgtctgtaaa actaaataat cttttctcac cagtttcaaa agccaaatgt
     7921 ttattacctt gtgcggtgta tcttctatgt gttttttcac tttcgatatc actcttattt
     7981 ttggctttat taatttcttt atttctaaca atatttgaaa gggttttggc aaaatttcca
     8041 ataaatggat gcatccctgg accgtccatt ttggagcctt ctaaaaaagc aacacccatc
     8101 accgcaggcg atgtaacgcc tccagtttta ccattattaa attctggcaa aatatcaatc
     8161 ttactaaaat ctatgtattg ttgaacaaag tcgattgtgt tattattaat ctgagtttga
     8221 ttattttcaa ctatttcttt tgccttttca tattgcaact gaccacagaa tttagagctt
     8281 ttcaaatcat cagaataaaa gccttcatat tttcctcttt ggaaaggcaa atttggatta
     8341 tatcgcacac gagcagagac atcaccgcaa gcaccttgag caaatgctcc aatataattt
     8401 ttatttttct tttgtaaatc acgctctaaa taatcagcgg cataaccttt attgtcagaa
     8461 cataatttta agaaattatt tggcaaacta ctggtatgta ccgcaaacca attaatagaa
     8521 cctagtaacg caccttcttc gcttacaaaa tttagcatag tcattgctct atccacagca
     8581 agatgtcttt ctgagaagga atattttttt acttcaggat tttggttgta agaatttacg
     8641 gttctattaa aagatactgg aatatcattg gcaaaattac tctttcctat tgtaatattt
     8701 gcttttactt ttactttatc tgcttcaaca atcgcttcaa caatgccttt taccaatttg
     8761 tctaaaatat gttgcacaaa acccggaaca gaaatattat acgttgcgtg gctagaaaat
     8821 cccgctggac tgcaatgtgt atgtgttgca gaaagcataa gattatcgtc tgtcaaacct
     8881 aaatcaggaa aatgctttgc aatataatct acaacagatt gttttaaaag tggtgtaata
     8941 aagcaaactt cacaattcac gatagcgatt ttattttgct tatcgatatc ctgaattaaa
     9001 taagcacgag caaataatgg cgtttcaaca tctaaggcaa tatgagaata cataccataa
     9061 cctagcatac ttgagccacg ttcaaatgca gtaatatctg cctttcctaa accaattaaa
     9121 aacatttttg taaatttggc aacaagataa taaaaagcaa aaaataatcc aagaaaataa
     9181 ttaactataa atgggtataa acaaagaaat atctgtaagt gtattgatga cagcctacaa
     9241 tgctgaaaac tatgttaaag aagcaattca gtcaatattg gaacagactt acagtgattt
     9301 tgagtttgta attataaatg atggttcaac agataagaca ctggaaatta ttcagtcttt
     9361 cgatgataaa agaataagaa ttatcaatga aggaaaattt ggttattaca aagcaaaacg
     9421 aagattgatt gaagaagcca aaggtaaata tattgccatt atggacgctg atgatattgc
     9481 tgataaaaat agatttgaag cagaagtaga ttttttggac aaacatccag actttggatt
     9541 ggtaggaaca aatgccacat ggattgataa agataataaa ccatttggaa aaggttttaa
     9601 ttttagttat tcacctgaag aattaaaatg cagactttta ttccataatt gttttgttca
     9661 ttcatcggtt ttgattagaa aatcaatatt agataaatat caactaaatt atagagaaaa
     9721 agctagtgaa gacttagacc tttgggtaca aatttctaga tatattgaag taaaaaatat
     9781 acccaaaaag ttaattaaat atcgcataca ttcaaacagt attgtgcata gtaattggta
     9841 taaaaaagac aatggattaa agcaaattac atttgaacaa ttagactatt attttcctga
     9901 aattctttca gtagaagaaa aaaatgcaat atttggttta gtgaattttg atgtagaaaa
     9961 tgacaataaa gttatgtcaa tcatacttaa atctattcag aagttgttac aatccaatca
    10021 gaagaacgag catttcaatg atattttttt cagaaaagta attcaagaaa gatggtttaa
    10081 aaaaattata cgcttaaaag aatattcgtc tgaaatatgg aaaacaaata aagaatgtaa
    10141 agaaatatta gatatcccac taacaccaaa acaacattta tttgagataa ttttaatttt
    10201 gaatataatt ttaaaaaaaa gaataattaa agtttaaatg cttattttaa gtataaaaag
    10261 taactatata aaaaaaacat tggtaggtta aatgtct
//
LOCUS       DAOQCW010000094        10248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_2473_length_10248_cov_5.036398, whole genome shotgun sequence.
ACCESSION   DAOQCW010000094 DAOQCW010000000
VERSION     DAOQCW010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10248
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2473_length_10248_cov_5.036398"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PLS16_08710"
     CDS             complement(<1..495)
                     /locus_tag="PLS16_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61201.1"
                     /translation="MQQFKIGENLQLKIYNSINEFNPIEWNSQIELLPFFQSFHYLQH
                     LEHTQKQINFRYILIFENQVLIGSIYLQQLNFDMKNIFQYNSKTKLTLVEEYFSKKPV
                     YLLYLGNVYFTGDTGIISNDISKLNLILPKIFKYADKTFLNKSFAYLSAENPTNKKVW
                     SGTHY"
     gene            572..1150
                     /locus_tag="PLS16_08715"
     CDS             572..1150
                     /locus_tag="PLS16_08715"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNJ61202.1"
                     /translation="MLGFIPGLIFANAFEWYFHKEILHGQGKKKGNFWRFHWAVHHKT
                     VLDENYRDSDYEKDIIESWNPQSKEVASLAVAAVAVAPFFPIAPGFVSALWLSQYNYY
                     RVHKKSHLDPEWGYKYLPWHYDHHMAPNQDMNWCVTFPLWDYVMGTRLVYKGTEREQL
                     DNQRRARKMQLKQEKLNKLKNINPDNLNISLN"
     gene            complement(1158..1595)
                     /locus_tag="PLS16_08720"
     CDS             complement(1158..1595)
                     /locus_tag="PLS16_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61203.1"
                     /translation="MKKLFFIFFAGLLFFVNAKPPKSNTSTTNNQSWVESTSIYGKWK
                     WASTECCGKKKGITTPDSNEDNIFLELKSDNTFKETSNKHRIPRQGTIILTKTYKEDK
                     SYTNMIQFNDERPAHYSLSKNGDTLTLSWEYLELQKETYVRVK"
     gene            1648..2028
                     /locus_tag="PLS16_08725"
     CDS             1648..2028
                     /locus_tag="PLS16_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002517182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNJ61204.1"
                     /translation="MFELEKVRVDKWLWSVRMFKTRTAATDACNSGKIKLDGTRIKPS
                     QLVKIGDELQFQQNGDIKILKVIRLIDKRVGAPIAITCYEDKSPPSIHDQSQPSFFYQ
                     YEIRDRGVGRPTKKDRRDIERFKD"
     gene            2100..2315
                     /gene="tatA"
                     /locus_tag="PLS16_08730"
     CDS             2100..2315
                     /gene="tatA"
                     /locus_tag="PLS16_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNJ61205.1"
                     /translation="MNSVLLFSMPGGGEWVLILLVVVLLFGGKKIPELMKGLGKGIRE
                     FNAAKNNIQEEFEKGLKEEEEKKKISE"
     gene            2512..4383
                     /locus_tag="PLS16_08735"
     CDS             2512..4383
                     /locus_tag="PLS16_08735"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNJ61206.1"
                     /translation="MTFKPIILCIFILFFAFTFDAKAQKLVNQWQDNPSDLLIPTEEQ
                     LFKNDPDPAILSAQINVRLKKIQRTIPLDYNAHVQKYIDFYTSKSTHIAKVLGRAEKF
                     FPIFEPIFNEYGVPDEMKYLAVVESALNPFARSPVGATGPWQFMHGTGLQYGLAINKR
                     IDERKDPYKSCDAAARYLKYMYNIYGNWHLAIAAYNCGPGNVNKAINKAGGSKNYWDI
                     KPYLPKETQAYVPSYIAIVYAMKYAKNMGISPSPLYRENTQRITLKDKLTVQDLTKIV
                     GIDKALIIEENPSLLTTLIPANFTINLPASKMVAFNYYQDSLYAFAENVNVGDKLSTP
                     NVVKPSADNTKSFSSEDIPKEKVEKPKKKTKQEIAKEKKLAEQEKLSAKKKLIEEEKK
                     LAEQKKKEKQSVKQEPTKEEYLKQKREELKQKNATAPIVQQNNLSKLQVANTQKIETP
                     DFYKEDDANYRTIVYTVMQGDNLGFIAKWFHVKVSEIRRWNELSGNLLKVNQELVLYV
                     HEKDYTHFVHFNFLSFRLKNILSDDIQTQEELLALKNKKEEKKSEPLSTKLNPINYFR
                     DEDCFEMYKIKSGETLWTIAQKYDEVSVQDLINWNDFDKKPILKPGQEIKVRKKPCK"
     gene            4401..4997
                     /gene="ruvA"
                     /locus_tag="PLS16_08740"
     CDS             4401..4997
                     /gene="ruvA"
                     /locus_tag="PLS16_08740"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNJ61207.1"
                     /translation="MYAYLKGKLTYKSPTLVYIECGGVGFQVNITLQTYSEIEKLEET
                     ILYTQQIVREDGHFLYGFYSEDEKNIFNHLISVSGIGPNTARLMLSSLSVQELTNAII
                     YEDVRLIQSVKGVGPKSAQRVIIELRDKVKKETYLQSSELGIMANSKSEIFSEAIAAL
                     SMLGFAKNQSEKAIQTVLKINPNINTTEDLIKQSLKNL"
     gene            5021..>10248
                     /gene="sprA"
                     /locus_tag="PLS16_08745"
     CDS             5021..>10248
                     /gene="sprA"
                     /locus_tag="PLS16_08745"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HNJ61208.1"
                     /translation="MKNTIRHIFFLLLLVGQQISIFAQKKDSIQLKFPIQDRNNNGIY
                     TPNSNNTLDLKDPKIINKDVEYDPITKQFYYTEKIGKSNFRTPTFLTYDEYVKLQNKN
                     TEQNYFQQRSKAIDLAERKTKQPFLYQGPELFDRLFAGTKIEIVPTGNVDVTVGVNSQ
                     KVDNPVLLQNQRRNTNFDFDMNIQLGLQASIGDKLKLGINYNSKAGFAFDNQLKLGYQ
                     GKEDDIIQLIEFGNISMPLRSALIRGPQSLFGIKSQLKFGRLTITNVLSQQKSKTENV
                     RFENGSQTKKFEIKADEYEDNRHFFLAHVFRDNYEQNLSRLPYVASQAVVTRLEVWVT
                     NKTRQTENVREVVAFADLGENKIQNVRNPSVLNGNADAYTGNYSNKIFGLLTANSELF
                     RNQAKVINFLTSNGFQSIDDYEKVSARKLATSEYTFNPQLGYISLNYQLRPDEVLGVA
                     VQYTVNGQVYQIGEFSNDLPPLADSAKSAERILALKMLKATSIRVKNPIWNLMMKNIY
                     SLNAYNVNREKFVFDIYYRDPGGGFKRYLPEAGDISGKQLLRVMNLDNLNNQNDPQND
                     GQFDFIPDITINTQNGKIIFPVLEPFGKWLADKINNPELAKKFAYQLLYDTTKVGAQQ
                     FPEFNRYVMKGTYQGTDNSTFRLPNAFQLPKGSIIVRSGGNVLTENVDYTVNYSIGEV
                     NIINQGVLSSGVPIDISFENNLMFGVVNKSLFGTRLDYTVNKNLTLGFTHMRLSEKPF
                     TQKVDFGNDPVKNNVWGMDLNYQGQSKSLSKFFNKITAQDITAPSKITAQAEVAQFIP
                     GHNKAVNNEGAGAVYIDDFEGASSSYDIKGSYLSWKLASAPKGMKDMYGQERFPEAKL
                     SDSLPYGFNRAKLAWYTIDPLFYDKSNRNPLSSSEMAKTNSNVYTRQFYSTEVYTNRQ
                     DPLLGNPPLGTLDLSYFPAERGPYNFETSPTSISKGTAPDGSLNNPETRWSGIMRSID
                     MSDFEAANIEYIQMWVLDPFVGVNSTDKKGSLYIQLGNISEDILKDSRKQYENGLPRP
                     NGGTTVERSAWGFTPTIVNAISNYFDADPKVIQKQDVGFDGMDDEQERDFYSTFLSKA
                     QLVVGDATAIQNLLDDPSSDNYIFPRDGSFSPDDDVITRYKNFSNTQGNSSNNNKGGD
                     VNGNSTNYPDNEDLNNDNTLNENEEYYQYKIAFDDQFIATSPYITDKVLRTATINGVN
                     QTYTWYQIKIPVADFEDKIGNIADFRSIRFIRMVMTDFEQPVTLRFSQISLIRNQWRR
                     YQLSLTQPGEELVNDQNNNTIFNITNVNIEENTNRKPITYALPPGITREQNISATSNN
                     NVQINEQSLSLQICRLEDGDARAIYKISDLDLRNYKQLKMFAHAENFVGGDGDLYPIK
                     DNDISLFMRIGADFTENYYEYEIPLKVTPPGTYNPNNDNDRRIIWPDSNEMKIAIDTL
                     THIKKLRNKDNAPNNLPYIYNTKNGKVTIIGNPDLGNAALLMIGVKNPKRISGINDNY
                     DDGNPKCAEVWVNELRVSGFDETKGWAALGRVDVQLGNLGNITLSGNMHTIGYGDIEQ
                     KVDQRNRDNFYQIDATANLQLGNLLPEKAGLKIPVYAQYSQAVSTPKYDPYEFDVKVK
                     DKYDEINADADLTKSEKKQQIDSIKNIVQDITTIKSINVTNLQKVRTNPDKQARIYDI
                     ENFTFTYAYTELEKTSPIVEYDRTVRHTGALGYTFTPKQFIWQPFKSLKNNNKYLKPI
                     KEFNLSLKPSSLSFRT"
BASE COUNT         3862 a         1409 c         1675 g         3302 t
ORIGIN      
        1 ataatgcgtg ccactccaca cttttttatt agttggattt tcagcagata aataagcaaa
       61 ggatttattt aggaaggttt tgtctgcata cttaaatatt ttaggtaaaa ttaagttgag
      121 ctttgaaata tcattgctaa taattccagt atcaccagta aaatacacat tgcctaaata
      181 taatagatat actggctttt tgctaaaata ttcttcaact aaagtaagtt tagttttaga
      241 attatattga aatatatttt tcatatcaaa attcaattgt tgtaagtaaa tactaccaat
      301 caatacttga ttttcaaaaa ttaaaatata tctaaagtta atttgttttt gagtatgttc
      361 taaatgctgt aaataatgaa aactttgaaa gaaaggaagt agctcaattt gactattcca
      421 ttcaatagga ttaaattcat ttatgctatt ataaattttt aattgtaagt tttctccaat
      481 tttaaattgc tgcataattt gaagaacaaa agtaaaattt aattttgtta attaatgttt
      541 tattcttaac ttcgattata aataattgat tatgttaggt tttattccag gattaatttt
      601 tgcaaatgct tttgaatggt attttcacaa agaaatatta catggacaag gcaaaaagaa
      661 aggtaacttt tggcgttttc actgggcagt tcatcataaa actgtattag atgaaaatta
      721 tagagattct gactatgaaa aagatattat agaatcttgg aatccacaaa gcaaagaagt
      781 ggcatcattg gctgttgcag cagttgcagt agcaccattt tttcctattg caccaggttt
      841 tgtttctgca ttgtggttat ctcaatacaa ttattatcgg gtacataaaa aatcacattt
      901 agatcctgaa tggggataca agtatttacc ttggcattat gatcatcaca tggcacccaa
      961 tcaagatatg aattggtgtg taacatttcc tctttgggat tatgtaatgg gaactcgttt
     1021 agtttataaa ggtacagaaa gagagcaatt agataatcaa cgtagagcaa gaaaaatgca
     1081 actcaaacaa gaaaaattaa ataagcttaa aaacataaat ccagataatt tgaatatttc
     1141 tttaaattag taacaaatta tttgactcta acataagttt ctttttgtaa ttccaaatac
     1201 tcccaactta aagttaaggt atctccattt tttgataaac tataatgagc tggacgttca
     1261 tcattgaatt gtatcatatt ggtatatgat ttatcttctt tatatgtctt tgttaaaatt
     1321 atagttcctt gtcttggaat tctatgttta ttagaagttt ctttaaatgt attgtctgat
     1381 tttaactcta aaaatatatt atcttcattt gaatctggtg tagtaatgcc ctttttctta
     1441 ccacaacatt cggtagatgc ccatttccac tttccataaa ttgaagtact ttccacccaa
     1501 ctttgattat tagtggtaga tgtatttgat ttaggtggtt ttgcatttac aaaaaataat
     1561 aatccagcaa aaaatataaa aaatagtttc ttcatatagc aaatttactt ttaattctta
     1621 atattgcata atgcaaaaac tttgccaatg tttgaattag agaaagttag ggtagataaa
     1681 tggttgtggt cagtacggat gtttaaaact agaaccgcag caactgatgc ttgtaattca
     1741 ggcaaaataa agttagatgg tacaagaata aagccttctc agttggttaa aataggagat
     1801 gagcttcagt ttcagcaaaa cggtgatatt aaaatattga aagtcataag gttaatagat
     1861 aaaagagttg gggcacctat tgctataaca tgttatgaag ataaatctcc gccaagcatt
     1921 cacgaccaaa gtcagccttc tttcttctat caatatgaaa taagagatag aggagttggg
     1981 cgaccaacta aaaaagatag acgtgatatc gaaaggttta aagattaaaa taaatcttaa
     2041 tcatttttat accttgtttt gattttaaaa tattaaattt gtaaagtaaa aatatagata
     2101 tgaattcagt tttgttattt agtatgcctg gaggcggtga gtgggtttta atattattag
     2161 tagtagtttt acttttcggt gggaaaaaaa ttccagaatt aatgaagggt ttaggtaaag
     2221 gtattagaga atttaatgct gctaagaata atattcagga agagtttgaa aaaggcctaa
     2281 aagaagaaga ggaaaaaaag aagatttcag aataaattat ttctaattcc ttcatttaaa
     2341 ctgtttttaa atacctaaaa cagtttattt atatttaata ctctacagtc cgaatttatt
     2401 ccctagaaat aattagtaaa taagaattat attattaatt atttcacaaa aaaaacatag
     2461 ataactgtat ttttgtttcc ctattttaaa atattaattt atattagtag aatgactttt
     2521 aagcctatta tactatgcat ttttatactt ttttttgcat ttacttttga tgctaaggca
     2581 caaaaattag ttaaccaatg gcaagataat ccttctgatt tattaattcc tactgaagaa
     2641 caattattca aaaatgaccc tgatccagcg attttaagtg ctcaaataaa tgttagacta
     2701 aaaaaaatac aaagaacaat acctttagat tacaatgcac atgttcaaaa atacatagac
     2761 ttttatacgt ctaaaagtac acatattgct aaagttctag gtcgtgccga gaagtttttt
     2821 ccaatatttg aacctatatt caatgaatat ggtgttccag acgaaatgaa atatttagct
     2881 gttgtagaat ctgctttaaa cccgtttgct cgttctccag ttggagcaac aggtccatgg
     2941 caatttatgc atggcacagg tttgcaatat ggattagcta ttaataagcg aattgatgaa
     3001 cgtaaagatc catacaaaag ttgtgatgct gctgctcgat acctaaaata tatgtacaat
     3061 atttatggaa attggcattt agctattgca gcctataatt gtggaccagg aaatgtcaac
     3121 aaagccatca ataaagctgg tggttctaaa aattattggg atataaaacc atatttgcca
     3181 aaggaaacac aagcttatgt tccttcatat attgcaatag tttatgcaat gaagtatgct
     3241 aaaaatatgg gcattagtcc atcgcctcta tatcgtgaaa atactcaacg cattacttta
     3301 aaagacaaat taactgttca agatttaact aaaattgttg gtatcgataa agcattaatt
     3361 atagaagaaa atccatctct attaaccact ttaatacctg caaattttac gattaattta
     3421 ccagcttcaa aaatggtggc ttttaattat tatcaagact cattatacgc atttgcagaa
     3481 aacgtaaatg taggagataa gttatctact ccaaatgttg taaaacctag tgcagataat
     3541 acaaagagtt ttagttctga agatattcca aaagaaaagg ttgaaaaacc taaaaagaaa
     3601 acaaaacaag aaattgccaa ggaaaagaaa ttagcagaac aagaaaagtt atctgcgaag
     3661 aaaaagctca ttgaagaaga aaaaaaacta gctgaacaaa agaaaaaaga aaagcaatct
     3721 gtaaaacaag agccaaccaa agaagaatat ttaaaacaaa aacgtgagga actaaaacag
     3781 aaaaatgcaa cggcaccaat tgttcagcag aataatctgt ctaaactgca agtagctaat
     3841 actcagaaaa ttgaaacgcc tgatttttac aaagaagatg atgcaaatta tagaactatt
     3901 gtttatacag tgatgcaagg cgataattta ggatttattg cgaagtggtt ccatgtaaaa
     3961 gttagtgaaa ttagacgctg gaatgaacta tctggaaatt tacttaaagt caatcaagag
     4021 ttagttcttt atgttcatga aaaagattat acccattttg tacactttaa ttttctttct
     4081 tttcgtttaa aaaatatttt atcagatgat attcaaactc aagaagaact gcttgcattg
     4141 aaaaataaaa aagaagaaaa gaaaagcgaa ccacttagta caaaattgaa tccaattaat
     4201 tattttcggg acgaagattg ttttgaaatg tataaaataa aatctggtga aacattatgg
     4261 acaattgctc aaaaatatga tgaggtttct gttcaagatt taattaattg gaatgacttt
     4321 gataaaaaac caattttaaa accaggacaa gaaattaaag tccgaaaaaa accatgtaaa
     4381 taacataaaa ctataaaaat atgtatgctt atctaaaagg aaaattaact tataagtcgc
     4441 cgaccttagt atatattgag tgcggtggag ttggttttca ggtgaatata accttgcaaa
     4501 cttattctga aattgagaaa ttagaggaaa ctattcttta tacacaacaa attgtccgtg
     4561 aagatggaca ttttctatat ggtttttata gtgaagatga aaaaaatatt tttaatcact
     4621 taatttcagt atcaggaatt gggccaaata cagctagact aatgttgtct tctttgtctg
     4681 ttcaagaatt gacaaatgca ataatttatg aagatgtacg tttaatacaa tcagtgaaag
     4741 gtgttggacc caaatcagct caaagagtaa ttattgaatt gagagataaa gtaaagaaag
     4801 aaacttattt acaatcttct gaattaggaa ttatggcgaa tagcaaatca gaaatctttt
     4861 cagaagcaat cgctgcttta tcaatgcttg gttttgcaaa aaatcaatca gaaaaggcaa
     4921 ttcaaactgt tcttaaaata aacccaaaca ttaataccac tgaagacctg ataaaacaaa
     4981 gccttaaaaa tttataattt taattatatt tgttcagact ttgaagaata ctattaggca
     5041 tatctttttt ctattgttac ttgttggaca acaaataagt atttttgctc aaaagaaaga
     5101 tagtattcaa ttaaaattcc caattcaaga tagaaataat aacggaattt atacacctaa
     5161 ttcgaataat actttagatt taaaagatcc taagataatc aataaagatg tagaatatga
     5221 cccgattaca aagcaatttt attatactga gaaaataggg aaatctaatt ttagaacccc
     5281 aacctttctt acttatgatg agtatgtaaa attacaaaat aaaaatactg aacaaaatta
     5341 ttttcaacaa cgttctaaag ctattgattt agcagaacga aaaactaagc aacctttttt
     5401 atatcaagga cctgaattat ttgatagatt atttgcaggc acaaaaattg aaattgtccc
     5461 gacaggaaat gtagatgtta cagtaggtgt aaattctcaa aaagtagata atcctgtttt
     5521 attacaaaat caaagacgaa ataccaactt tgattttgat atgaatatcc agttgggttt
     5581 acaagcttct attggtgata aattaaaatt aggcattaat tacaattcaa aagctggttt
     5641 tgcatttgat aatcaactaa aattagggta tcaaggcaaa gaagacgata ttatacaatt
     5701 gatagaattt ggtaatataa gtatgccatt acgctctgct ttgatacgtg gcccacaaag
     5761 tttatttggt attaaatctc aattgaaatt tggaagactt acaattacaa atgttttgtc
     5821 ccaacaaaaa tctaaaactg aaaatgttcg atttgaaaat ggttctcaaa ctaaaaaatt
     5881 tgaaatcaaa gctgatgaat atgaagataa tcgacacttc tttttagccc atgtttttag
     5941 agataattat gaacaaaatt tatcccgttt gccttatgtt gcttcccaag ctgttgtaac
     6001 caggttggaa gtttgggtta ccaacaaaac aagacaaaca gaaaacgtac gtgaagttgt
     6061 ggcatttgcc gacttaggtg aaaataaaat acaaaatgta agaaacccaa gtgtgttgaa
     6121 tggaaatgct gatgcttata caggaaatta ttctaataag atttttggtt tattgaccgc
     6181 taattctgag cttttccgaa atcaagcaaa agtcattaat tttttaacct ctaatggctt
     6241 tcaatctatt gatgattatg agaaagtaag tgcaagaaag ttagcgacat ctgaatatac
     6301 atttaatcca caattaggtt atatttcatt aaattatcaa ttacgtccag atgaagtttt
     6361 gggtgtggct gtacaatata ctgtgaatgg acaagtgtat caaataggag agttttctaa
     6421 tgatttgcca ccattagcgg attctgctaa atctgcagag cgaattttag ctttgaaaat
     6481 gttgaaagca acttctatta gagtaaagaa tccaatttgg aatttaatga tgaaaaatat
     6541 ttattcatta aatgcctaca atgtcaatcg tgaaaaattt gtatttgata tttattatag
     6601 agacccaggt ggtggattta aaagatacct tccagaagct ggagatattt caggcaaaca
     6661 attactgaga gtaatgaatt tagataacct aaataaccaa aacgacccac aaaatgatgg
     6721 ccaatttgat tttattcctg atattaccat aaatacccaa aatggtaaaa ttatttttcc
     6781 agttttagaa ccttttggca aatggttggc agataaaatt aacaaccctg aattggcaaa
     6841 aaagtttgct tatcaattat tgtatgatac aacaaaagtt ggagctcaac aatttcctga
     6901 atttaataga tatgtaatga aaggaactta tcaaggcact gataatagta ctttccgatt
     6961 gccaaatgca tttcaattgc ctaagggttc gataattgtt cgttctggcg gaaatgtgct
     7021 tactgaaaat gttgattata cagtcaatta tagtattggc gaagtaaata ttatcaatca
     7081 aggtgtatta agttcaggcg ttccaataga cattagtttt gaaaataatt taatgttcgg
     7141 cgttgtcaat aaatcattat ttggaactag gttagattat accgtcaata aaaatttaac
     7201 attaggtttt acacatatgc gtttatcgga aaaaccattt actcaaaaag tagatttcgg
     7261 aaatgaccca gttaagaata atgtttgggg aatggatttg aactatcaag gtcaatcaaa
     7321 aagcctatct aaattcttta ataaaattac tgctcaggat atcacagcac catctaaaat
     7381 tacggcacaa gcggaggtgg cacaatttat cccaggtcat aataaagcag ttaataacga
     7441 aggtgcaggt gcagtatata ttgatgattt tgaaggtgca agttcttctt atgatattaa
     7501 aggaagttat ttgtcgtgga aattagcttc agcacctaaa ggaatgaaag atatgtatgg
     7561 acaagaacga tttccagaag ctaaattgag cgatagttta ccttatggtt ttaatcgagc
     7621 aaaattagct tggtacacga tagatcctct attttatgat aagagcaata gaaatccttt
     7681 aagttcaagt gaaatggcca aaacgaatag taatgtttac acgaggcaat tctattcaac
     7741 tgaagtctat accaatagac aagacccact tttaggaaac ccacctttag gaactttaga
     7801 tttatcttat tttcctgcag aaagaggtcc ttataacttt gaaacatcgc ctacttcaat
     7861 atcaaaagga actgcacctg atggttcttt aaataatcct gaaacaagat ggagtggaat
     7921 tatgcgttct attgatatga gtgattttga agctgcaaat attgaatata ttcaaatgtg
     7981 ggttctcgat ccttttgttg gcgtgaatag tactgataaa aaaggtagtt tatacattca
     8041 attaggaaat atttctgaag atatattaaa ggattctcga aagcaatatg aaaatggttt
     8101 gccaagacct aatggcggaa caactgtaga gcgttctgct tggggcttta cgccaacaat
     8161 tgtaaatgca attagtaatt attttgatgc agatccaaaa gtaattcaaa aacaagatgt
     8221 tggttttgat ggaatggatg atgaacaaga aagagatttt tatagtactt tcctttctaa
     8281 agctcaattg gttgtgggtg atgcaaccgc aattcaaaat ctactagatg acccatcatc
     8341 tgataattat atatttccta gagatggtag cttttcaccc gatgatgatg taattacacg
     8401 ctataaaaat tttagtaata ctcaaggaaa ctcttctaat aataacaaag gaggagatgt
     8461 aaatggcaat tcaacgaatt atcctgataa tgaggatttg aataatgata atactttgaa
     8521 cgaaaatgaa gaatattatc aatataaaat agcttttgat gaccaattta ttgcaacttc
     8581 accatatatt acagataaag tattaagaac tgcaactatt aatggtgtaa atcaaactta
     8641 tacttggtat caaattaaaa tacctgttgc tgattttgaa gacaaaattg ggaatattgc
     8701 tgacttccgt tcaattcgat ttatacgtat ggtaatgact gattttgaac aacctgttac
     8761 cttacgtttc tctcaaatat ctttaatcag aaaccaatgg agacgctatc aattatcatt
     8821 aactcaacca ggcgaagaat tagtaaacga tcaaaataat aatacaattt tcaatattac
     8881 aaatgtaaat attgaagaaa ataccaaccg caaacctatt acttatgcac ttcctccagg
     8941 tatcacacgt gaacaaaata ttagtgcaac ttccaataac aatgtacaaa taaatgaaca
     9001 atctttgagt ttacaaatct gtagattaga agatggcgat gctcgtgcta tttataaaat
     9061 ttctgattta gatttaagga attataaaca gctaaaaatg tttgctcatg ctgagaattt
     9121 tgtgggtgga gatggcgatt tatatccaat aaaggataat gatatttcat tatttatgag
     9181 aataggagct gattttactg agaattatta cgagtatgaa attccactaa aagttacacc
     9241 tccaggtact tacaatccaa ataatgataa tgatagaaga attatctggc cagattccaa
     9301 tgaaatgaaa attgccattg atacattaac gcatatcaaa aagttaagaa ataaagacaa
     9361 tgcacctaat aatttacctt atatctataa tactaaaaat ggcaaagtaa caattatagg
     9421 aaatcccgat ttaggcaatg ctgctttgtt aatgataggt gtgaaaaatc caaagagaat
     9481 cagtggaatt aatgataatt atgatgatgg aaacccaaaa tgtgccgaag tttgggtaaa
     9541 cgaattaaga gtgagtggtt ttgatgaaac caaaggttgg gctgcattag gacgtgtaga
     9601 tgttcaatta ggaaacttag gaaatattac actttctgga aatatgcata caattggtta
     9661 tggtgatatt gaacaaaaag tagatcaaag aaatagagat aatttttacc aaattgatgc
     9721 tactgcaaat ttgcaattag gcaatttact tccagaaaag gctggtctaa aaattccagt
     9781 gtatgcacag tattcacaag ctgtaagtac accaaaatac gatccttatg agtttgatgt
     9841 gaaagttaaa gataaatatg atgaaattaa tgcagatgct gatttgacaa aaagtgaaaa
     9901 aaagcaacaa attgattcga ttaaaaatat tgtgcaagat attacgacaa ttaaaagcat
     9961 caacgtaacg aatttacaaa aagttagaac caatcctgat aaacaagcaa ggatttatga
    10021 tattgagaat tttacattta cttatgctta tactgaattg gagaagacaa gtccaatcgt
    10081 agaatatgat agaacagtga gacatacagg tgctttagga tatacattta cgccaaaaca
    10141 atttatttgg caaccgttca aatctttaaa aaataacaat aagtatctaa agcctattaa
    10201 agaatttaac ctaagtttga agccttcttc cttatcattt agaacaga
//
LOCUS       DAOQCW010000095         9949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_2576_length_9949_cov_5.425510, whole genome shotgun sequence.
ACCESSION   DAOQCW010000095 DAOQCW010000000
VERSION     DAOQCW010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9949
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2576_length_9949_cov_5.425510"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..507
                     /locus_tag="PLS16_08750"
     CDS             61..507
                     /locus_tag="PLS16_08750"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HNJ61209.1"
                     /translation="MNVISWGKGITLVILLFIVGIGYLVYRTTIVKSEMVTDNYYEKE
                     LKHEEVLQAERNALNLSALPKVELQTNQIVVDFPKEMMPTKILGDIVFYKPNKSADDK
                     SYPISLSADNTQVINLSELAKGVYQIQIKWKQDEVPYYFQNDIYIP"
     gene            507..1226
                     /locus_tag="PLS16_08755"
     CDS             507..1226
                     /locus_tag="PLS16_08755"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNJ61210.1"
                     /translation="MIYALTSGFLLGLMGSFHCMGMCGPIALALPVHKHNTLKKVWYI
                     LMYNFGRIFTYSVLGFLFGLLGKGLFIGKYQQYFSIAIGVLMLLALVVPTLFKKADNK
                     YIAQFSAFVRKQLSNLFKKEKSGSVYLTIGIFNGLLPCGLVYFAIASAVGMGNLENTV
                     GFMFMFGLATIPMMFAIAFLGNFISQKFRQTIQRLIPFFIAIMAVLMVLRGLNLGIPY
                     LSPKIEHTEHGTEQSCCHPEE"
     gene            1271..2143
                     /locus_tag="PLS16_08760"
     CDS             1271..2143
                     /locus_tag="PLS16_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61211.1"
                     /translation="MTNKQIYTNFIEKHRDIYIPIFMQPFWLDNFSENWDVVLYQING
                     QIVAALPYSMKGNLLTKRIYLPYVNFYQSILFFESFDVKTTTKIANQLFTQLPKTIKQ
                     YFKFLPEYLDIDLTKLKYKKSLYSSYILKENPDYSNHHLRKINKAKIENYQIIRHKYV
                     KEAYNLIAQTFTRQKVKLKLTLSDFEHLNKIISKYNCGTLYTCLDKEHNILATALIVE
                     DNRVAYYLFGAFNRQFKNSGAMHYLLNNIALNTHQQNKILNFCGSSKPSIAQFFKGFG
                     AKPFDIPIWKKTIF"
     gene            complement(2140..2868)
                     /locus_tag="PLS16_08765"
     CDS             complement(2140..2868)
                     /locus_tag="PLS16_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61212.1"
                     /translation="MKYFFSVCIIIALPLVAFSQTKSNDEVKKIIPTLWLSILDKTNS
                     AKTLNIKSLNYYQIPKELDFKGIVVDAIKWNDKEGEKLVILSMTGLFEAKEYGADSLN
                     YILHNKSEVYAYLFLKKTNYDTYSKIWRISESNDCYSDNWFTGFIPQSATITDLDEDG
                     ISELTLPYLLLCRTKNETGKLNILMYEGKDKYEVSGNTIINCNQANSEKGVFSKSDNL
                     SYRPVFSDFLKKRWNEHQCENGRF"
     gene            3024..4097
                     /gene="serC"
                     /locus_tag="PLS16_08770"
     CDS             3024..4097
                     /gene="serC"
                     /locus_tag="PLS16_08770"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNJ61213.1"
                     /translation="MKKHNFGAGPSILPQTVFEQAAKACIELDNIGLSLLEISHRSKE
                     FDAIINEAIALIKELLNVPNGYSVLFLQGGANMQFSMIPYNLLGENEMAAYLDTGVWA
                     SKAEKEARLYGNTEIVASSKDANYNFIPKNYTIPDNAKYFHITTNNTIYGTEILEDLQ
                     INVPLIADMSSDIFSRPLDVSKYALIYAGAQKNLGIAGVTLVIIKDELLAWSPRKKST
                     IWDYKVFADNNSLFNTPPVFAIYSCLLTLRWLKNLGGLAEMEKINIAKSTLLYNEIDR
                     NPIFVGHAVPEDRSRMNINFVAKDKADETAFLEFIQAYNIHGLKGYRTVGGFRASCYN
                     ALPLESVQVLVQAMQDYEKSKLG"
     gene            4140..4739
                     /gene="bioD"
                     /locus_tag="PLS16_08775"
     CDS             4140..4739
                     /gene="bioD"
                     /locus_tag="PLS16_08775"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HNJ61214.1"
                     /translation="MQVFVSGIHTDIGKTIVSSILVKSLNADYWKPVQAGGLSNTDAD
                     IVRKFNPNSKIFPSQYNLLNATSPHFAAIQENININIEDFVFPNSENLIIEGAGGLLS
                     PLGNTFTNLDFIKFYKLPFVLVVKHYLGSITHTLSCLKIIENERLNFLGIIYVGNNEN
                     LTEEFIDNYATSKVLGRINIVDTITDNFILQSSQYLKIF"
     gene            4742..5998
                     /gene="bioA"
                     /locus_tag="PLS16_08780"
     CDS             4742..5998
                     /gene="bioA"
                     /locus_tag="PLS16_08780"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: protein motif:HMM:NF004624.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HNJ61215.1"
                     /translation="MDWINYDENIWHPFTQHALEKNRIAITHGKDSLLYDVNGKAYID
                     AISSWWVNIYGHANPTISNAIKLQVDKLEQVIFAGFTHEPACLLSENLLKILPSNFSK
                     VFFSDNGSTSVEVALKMAVQFWHNQGIKKSKIIAFKNAYHGDTFGAMAVGKSDFFSAF
                     DDLLFDVIQLDIPQVNNFESLKNEFQSLVETKDVACFIFEPLVQGSGGMQIYDVIYLD
                     ELISIARANEVICIADEVMTGFGRTGERFAIDYLENKPNIICLSKGITGGFMSLSVTV
                     CEKEIYDAFYSSDKLKTLFHGHSYTANLLGCAAANASVNLLEEFYPKIKQLAAWQKEK
                     VQELAVLPNVKNIRTLGTIAAFEIESTSTSYFSNVKDIVYQRFLDNGILLRPLGNTIY
                     VMPPYCITINELNEIYKVILDVVASL"
     gene            complement(6206..7948)
                     /locus_tag="PLS16_08785"
     CDS             complement(6206..7948)
                     /locus_tag="PLS16_08785"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HNJ61216.1"
                     /translation="MSYQTPISIKQAIDKIDFNHYLLPSIQREFVWGSGQIELLFDSL
                     MRNYPIGSLLMWKVQGVNKTDHRFYSVLKNYREKYSIHCKEVNTNSIADFEAVLDGQQ
                     RLTALYIGLNGSYAYKKKNFAWKDNEHSLPTRKLYLCLDQDTIDDDENEDGRVYDFRF
                     LTAEEISTSDKIWFEVGRILALNGTFELNQFLKKNEWDSNQYITNTLSKLLDVIHVKT
                     LINYYLELEQDYDKALNIFIRINSGGEKLSYSDLIMSTIISGWTSKTVSARDEFNNLI
                     DEIWNSTEIVIDKDLIIRTYLLIFSDDIKFRVTNFSIANAIEFKENWTDIRAAIVEAI
                     ELVKDFGYVERTLTSKNALLPLIHYLFISGRTKQFTTKVAYKEDRELIKRWFHTVLLK
                     RIFGGQADTVLKIIRDVVKDEIKKGADIFPATVIAKKLSKTRKSITMDDESIENLLFT
                     TYEDRYAFPILALLYPHLDYKNNDFNKDHIFPVIQFNSKNLKKSNVDLTQENGHYYTD
                     NWCYNGIVNLQMLDSNENKSKSDKTFEEWAKSTKINYEKQILPKITDFNKYIDFVDAR
                     WDMLKEKLEKALKF"
     gene            complement(8154..8486)
                     /locus_tag="PLS16_08790"
     CDS             complement(8154..8486)
                     /locus_tag="PLS16_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNJ61217.1"
                     /translation="MATKNKELEMFSFDQIKDEFIGEIGTKKRTQYEQELQLEVLGEM
                     IRKVRLERNLTQEELGKLVGVQRAQISKLENNTTNVTMETILKVFGALKAKVNFNVVL
                     MNNRIQIA"
     gene            complement(8470..8829)
                     /locus_tag="PLS16_08795"
     CDS             complement(8470..8829)
                     /locus_tag="PLS16_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNJ61218.1"
                     /translation="MNKFEVIFLTEAREFLLQLDEKSRDKIIFNIDKAKIKNDNDLFK
                     KLKGEIWEFRTLFTKTHFRIFSFWDKEDKQQTLVLATHGIIKKTDKTPEKEIEKAEQI
                     RLKYFELKNIRKNGNKK"
     gene            complement(9083..9847)
                     /locus_tag="PLS16_08800"
     CDS             complement(9083..9847)
                     /locus_tag="PLS16_08800"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR04274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HNJ61219.1"
                     /translation="MTLHEAIEKLLRQTGRPMTTQQIADELNKNGWYQKKDGSAIQAF
                     QIHGRTRNYANIFDRDGSTVSLIRQAVTKVKPATTSQPKTVKQIVKATSTNTKTSFDP
                     ISNSDTTILILGTMPGDKSLALGEYYGHARNRFWKIISTITDNDLPLTYTDKKALLLK
                     SKIGIWDVAHKANRKGSLDSAIEDEEPNDLDSFIARHKNLKIIGFNGAKSQALFDKYF
                     DRQSDLKYISLPSTSPANTGIDFDNICKQWRQLLTI"
BASE COUNT         3108 a         1468 c         1632 g         3741 t
ORIGIN      
        1 agggtaaaaa aataaataca attaaaaccg catttttagg accttttatg taatattatt
       61 atgaatgtaa tcagttgggg aaaaggaatc actttagtta ttttactgtt tattgtaggt
      121 ataggctatt tggtgtatag aacaacaata gttaaatctg aaatggtaac ggataattat
      181 tatgaaaaag aactaaagca cgaagaggtt ttacaagcgg aaagaaatgc tctaaattta
      241 agtgctttgc ccaaagttga attgcaaact aatcaaattg tagtcgattt tcctaaagaa
      301 atgatgccaa caaagatttt aggagatata gtattttaca agcctaacaa atctgctgat
      361 gataaatctt atccaatttc attatctgct gataatacac aagtaattaa tttgtcagaa
      421 ttggctaaag gtgtttatca aattcagatt aaatggaagc aagatgaagt gccgtattat
      481 tttcaaaacg atatatacat accttaatga tttacgcatt gacatctggt tttttgctgg
      541 gtttaatggg tagttttcat tgtatgggta tgtgtggacc aattgcatta gcacttcctg
      601 ttcataaaca taatacatta aagaaagtat ggtatatttt gatgtataat tttggcagaa
      661 tctttactta ttctgttttg ggctttctct ttggattatt aggaaagggt ttgtttatag
      721 ggaaatatca acaatacttt tctattgcca ttggtgtttt gatgttattg gcattagttg
      781 tacctacttt atttaaaaaa gctgataata aatatattgc tcagtttagt gcttttgtgc
      841 gtaagcaatt gtcaaattta tttaaaaaag aaaaaagtgg ttcagtttat ttaacgatag
      901 gtatttttaa tggattattg ccttgtggat tagtatattt tgccattgca tctgctgtcg
      961 gtatgggcaa tttggaaaat acggttggct ttatgtttat gtttggttta gcaacgattc
     1021 ctatgatgtt tgcaattgct tttttgggta attttatttc tcaaaaattt agacaaacta
     1081 ttcaacgttt aatacctttt tttatcgcaa taatggcagt tttaatggtt ctacgaggct
     1141 tgaacttggg tattccttat ttgagtccta agatagaaca taccgaacac ggaactgaac
     1201 agtcttgctg tcatcctgaa gaatagtatt tgttgttagg aatgtttatt tttacttaca
     1261 aaatcttaca ttgaccaata aacagattta tactaatttt atagaaaaac atagagatat
     1321 ttatattcct atttttatgc aacctttttg gttggataat ttctctgaaa attgggatgt
     1381 cgttctgtac caaattaatg ggcaaattgt tgcggcttta ccttattcaa tgaaaggtaa
     1441 tttattaacc aaaagaattt atttgcctta tgtaaacttc tatcaatcta tcctattttt
     1501 tgaatcattt gatgttaaaa caactacaaa aattgctaat caattattta ctcaattacc
     1561 taaaacaata aaacaatatt ttaaattttt gccagaatat ttagatattg acttaactaa
     1621 attaaaatat aaaaaatctt tgtactcatc ttatatattg aaagaaaacc ctgattattc
     1681 taatcatcat ctacgaaaaa ttaataaggc taaaattgaa aattatcaaa ttattcgaca
     1741 taaatatgtc aaagaagctt ataatttgat tgctcaaaca tttactcgac aaaaagtgaa
     1801 attaaaatta acattatcag attttgaaca tctaaataaa ataattagca aatataactg
     1861 tggaacttta tatacttgtt tggataaaga acataatatt ttggctaccg cattaattgt
     1921 agaagataat cgagtggctt attatttatt tggtgctttt aataggcaat ttaagaatag
     1981 tggagctatg cactatttgt taaataatat tgctttaaat acacatcaac aaaataaaat
     2041 tttaaacttt tgtggaagct caaaacctag cattgctcag ttttttaaag gttttggagc
     2101 aaaaccattt gatataccaa tttggaaaaa aactattttt taaaatctac cattctcaca
     2161 ttgatgttca ttccaacgct tttttaaaaa atctgagaaa acaggtctat aacttaaatt
     2221 atcacttttt gaaaagacac ccttttctga atttgcttga ttacaattta ttatagtatt
     2281 gccactgact tcgtatttgt ctttcccttc atacatcaag atattcaatt ttccagtttc
     2341 attttttgta cgacaaagca ggagataagg taaggtcaat tctgaaattc catcttcatc
     2401 taaatccgta attgtcgcag attgtggaat aaaaccagta aaccaattat cgctgtaaca
     2461 atcattggat tcacttattc gccaaatttt agaataggta tcataattag tttttttcag
     2521 aaatagatag gcatatactt ccgatttgtt atgcaatata taattaagcg agtctgctcc
     2581 atattcttta gcttcaaata agccagtcat acttaatatg actagctttt cgccttcttt
     2641 gtcattccat tttattgcat cgacaacaat ccctttaaaa tctaattctt tgggaatctg
     2701 ataataattt aaagatttta tgtttaatgt cttagcagaa ttggttttgt ctaatatgga
     2761 taaccataat gtaggtatta ttttctttac ttcatcattc gatttagttt gagagaatgc
     2821 tactaatggt aaagcaataa ttatacaaac cgaaaaaaaa tatttcataa ctcgtatttt
     2881 taggtttaat gaagttaaat agaaatatgt tgattttaaa attttttatt ttaaaattta
     2941 tttttgtgct gagatactaa aattaatatt caaataatgc ctatccaact tgaattcttt
     3001 taaataactg taatttagtt tttatgaaaa aacataactt tggtgctggg ccttctattt
     3061 tgccacaaac agtttttgaa caggcagcaa aagcttgtat tgaattagat aatattggct
     3121 tgtctttatt ggagatttct catagaagta aagaatttga tgcaattatt aatgaagcta
     3181 ttgctttaat taaagaatta ttgaatgttc caaacggcta ctccgtactt tttttacaag
     3241 gtggtgcaaa tatgcaattt tcaatgattc cgtataattt attaggcgaa aatgaaatgg
     3301 ctgcttattt agatactggc gtttgggctt ctaaagctga gaaagaagct agattatatg
     3361 gaaatactga aattgttgcg agttcaaaag atgccaacta taattttatt ccaaaaaatt
     3421 atacaattcc tgataatgca aagtattttc atatcacaac gaataatacg atttatggta
     3481 ctgaaatatt agaagatttg caaataaatg ttcctttgat tgcagatatg agctccgata
     3541 tttttagtcg cccgcttgat gtttcaaaat atgcattaat ttatgctggt gcacagaaaa
     3601 atttaggtat tgctggtgtt actttagtta ttataaaaga tgaattgcta gcgtggtcgc
     3661 ctcgtaaaaa atctactatt tgggattata aagtttttgc tgataataac tcattattca
     3721 atacaccacc tgtttttgct atttattctt gtcttttgac tttacgttgg ttgaagaatt
     3781 taggaggatt agctgaaatg gaaaaaataa atattgctaa atctacttta ttgtacaatg
     3841 aaatagatag aaatccaatt tttgtaggtc atgctgtgcc agaagacaga agtagaatga
     3901 atattaattt tgttgcaaaa gataaagccg atgaaacagc attcttagag tttattcaag
     3961 cttataatat tcatggattg aaaggttatc gaacagttgg tggatttaga gcatcttgtt
     4021 acaatgcctt gccattggaa agtgttcagg tattggtaca agccatgcaa gattatgaaa
     4081 aaagtaagtt aggataattt gaatactttc aaatcatttt atttgaaaaa aatgtactca
     4141 tgcaggtttt tgtttcggga attcatacag atattgggaa aacaatagtt tcatctattt
     4201 tagttaaaag cttaaatgct gattattgga aacctgtaca agctggtgga ttgagtaata
     4261 cagatgctga tattgttcga aagtttaatc ctaattctaa aatttttcca tcacaatata
     4321 atttgctaaa tgcaacttcg ccacattttg cagcaatcca agaaaatata aatattaata
     4381 ttgaagattt tgtatttcca aattctgaaa acttaattat tgagggtgct ggtggattat
     4441 tgtcgccatt aggaaataca tttacgaact tggattttat aaagttttat aaattgccat
     4501 tcgtgttggt tgttaaacat tatttaggta gtattacaca tactttaagt tgtctgaaaa
     4561 taatagaaaa tgagcggttg aattttttag gtataatata tgtcggtaac aatgaaaact
     4621 taacggaaga atttattgac aattatgcaa cttcaaaagt tttaggtaga attaatattg
     4681 ttgatacaat tacagataat tttattctac aatcttctca atacttgaaa atattttaaa
     4741 aatggattgg attaattatg atgaaaatat ttggcatcca tttacacaac atgcattaga
     4801 gaaaaataga attgcaatta cgcatgggaa agattcatta ttgtatgatg ttaatggaaa
     4861 ggcttatatt gatgcgattt cttcttggtg ggtaaatatt tatggtcatg cgaatccaac
     4921 catttcaaat gctataaagc ttcaagtcga taagttagaa caagttattt ttgcaggatt
     4981 tacccacgaa ccagcttgtc ttttatcaga aaatctatta aaaatcttac cgtcaaattt
     5041 ttctaaagtt tttttttcag ataacggctc tacatcggta gaagttgcct tgaaaatggc
     5101 tgttcaattt tggcataatc aaggaattaa aaaatcaaaa ataattgcgt tcaaaaatgc
     5161 ctatcacgga gatacatttg gagctatggc agtcggaaaa tctgattttt tttcagcttt
     5221 tgatgatttg ttatttgatg ttatacagct agatattcca caggttaata attttgaatc
     5281 cttaaaaaat gaatttcaat ccttagtaga aactaaagac gtggcttgtt ttatttttga
     5341 gccattagta caaggctctg gcggaatgca aatatatgat gtaatttatt tagatgagtt
     5401 gataagcatt gcgagagcaa atgaagtgat ttgtattgcc gatgaagtga tgactggttt
     5461 tggtaggaca ggagagcggt ttgcaattga ttatttagaa aataaaccga atattatttg
     5521 tttatccaaa ggaattactg gaggatttat gtcattgagt gttacggttt gtgaaaaaga
     5581 aatctatgat gctttttatt cttcagataa gttaaaaact ttgtttcacg gacattccta
     5641 tactgcaaac ttattaggtt gtgcagcagc caatgcatca gtaaatttat tagaagaatt
     5701 ttatccaaaa attaagcaat tagcagcttg gcagaaagaa aaagtacagg aattggcggt
     5761 tttgcctaat gtaaaaaata tcagaacttt aggaactata gccgcatttg aaattgaaag
     5821 tacttcaact tcttacttta gtaatgtgaa agatattgtt taccaacgat ttttagataa
     5881 tggaattttg cttcgcccac ttggaaatac tatttatgta atgccacctt attgtattac
     5941 aatcaatgag ttaaatgaaa tttataaagt tatacttgat gtagttgctt cactttgata
     6001 gtatatatta atttatataa tataaaatta ttttttgcaa ttatttttca gtattatgcc
     6061 taaaactaaa aaggagtcgc tgaacaatct cgcttaaagc aatacaaaaa gttctattcc
     6121 tacgctcagg gctttacttt tcaggacttt cttctattcg tcataatgtt atgtgctggc
     6181 gttttggtaa ttcgttgctc agtcgttaaa atttaagtgc tttttctaac ttctctttca
     6241 acatatccca ccgtgcgtcc acaaagtcta tgtatttatt gaagtctgta attttcggta
     6301 aaatttgttt ttcgtaattt atttttgttg actttgccca ctcttcgaaa gtcttgtcac
     6361 ttttagattt gttttcatta ctgtcaagca tttgaaggtt tacaattcca ttgtaacacc
     6421 agttgtcagt atagtaatgc ccattttctt gtgttaagtc aacgttcgat ttctttaggt
     6481 ttttactatt gaattgaatt actgggaaaa tgtggtcttt gttaaagtcg ttgtttttgt
     6541 agtctaaatg tgggtaaagt aaagctaata ttgggaacgc atatcggtct tcatacgttg
     6601 tgaaaagtaa attttcaata gattcatcat ccattgtaat tgactttctt gtctttgaaa
     6661 gtttctttgc aattacagtt gctggaaaaa tatcagcccc ttttttgatt tcatctttta
     6721 caacatctct gataattttt aaaacggtat ctgcttgtcc accaaatatt cgtttgagta
     6781 aaactgtatg aaaccatctt tttattaact ctctgtcttc cttgtaggct acttttgttg
     6841 taaactgttt cgttctccca cttatgaata aatagtgaat taaaggtaac aacgcatttt
     6901 ttgatgtcaa tgttctctct acataaccaa aatctttaac aagttcaatt gcttcaacta
     6961 ttgctgctct tatgtctgtc cagttttctt taaactcaat tgcatttgca attgaaaaat
     7021 ttgttaccct aaatttaatg tcgtcactaa atattagaag gtatgttcta atgattaagt
     7081 ccttgtcaat tacaatttct gttgagttcc aaatctcatc aattagattg ttgaattcgt
     7141 ctcttgctga aactgtcttt gaagtccatc cagaaattat tgttgacata attaagtcgg
     7201 aatacgaaag tttttcaccg ccactattta tgcgaatgaa aatattaagt gccttgtcgt
     7261 aatcttgttc taactccaaa taataattta tcaaagtctt aacgtgaatt acgtcaagta
     7321 gttttgacaa ggtgtttgtt atgtattgat ttgaatccca ttcatttttt ttgagaaatt
     7381 gatttagttc aaaagttccg tttagtgcta aaatacgtcc tacttcaaac caaattttat
     7441 cagaagttga aatttcttca gccgttagaa atctgaaatc gtaaaccctt ccatcttcat
     7501 tttcatcatc gtcaattgtg tcttggtcta aacagagata taattttctt gtcggaagtg
     7561 aatgttcatt gtctttccaa gcaaaatttt tcttcttata agcatatgaa ccattcagcc
     7621 caatgtataa tgctgttagc ctttgctgtc cgtccagaac agcttcaaag tctgcaattg
     7681 aattagtatt aacttctttg caatgaatac tatatttttc tctgtagttt tttagaacgc
     7741 tataaaacct atggtctgtt ttgtttacgc cttgaacttt ccacattagc aaagaaccaa
     7801 ttggatagtt tctcattaaa ctgtcaaaca aaagttcaat ttgtcctgaa ccccaaacaa
     7861 attctctttg aattgaaggt aacaaataat ggttgaagtc aattttgtcg attgcttgtt
     7921 ttattgaaat aggtgtttga tagctcattt atttacagtt gttaatgatt gtttgttgtc
     7981 tgctgtgtcg ggtctgttac gcttgcacat aacgttttgg cagcttggcg atgtggcggc
     8041 aatttagcac aaatgttgaa tagaattact accgttcaac cttgcacaaa agctcaatag
     8101 aagtacttct ccagctctat tgccaatttg tttgttggca gaagtagctc tcatcaagcg
     8161 atttgtattc tgttgttcat caataccaca ttgaaattta ctttggcttt taatgctcca
     8221 aaaactttca atatagtttc cattgttaca ttagttgtgt tgttttcaag ttttgaaatt
     8281 tgcgcacgtt gcactccaac caactttcca agttcttcct gagttaaatt tcgttcaagt
     8341 cggacttttc ttatcatttc acccaatact tctaattgaa gctcttgttc gtattgagtt
     8401 ctctttttag tgccaatttc accaataaat tcgtctttta tttggtcgaa actaaacatt
     8461 tctaattctt tattttttgt tgccattttt tctaatattt ttaagttcaa aatatttcaa
     8521 tctgatttgt tcagcttttt caatttcttt ttcaggtgtt ttatctgttt tctttatgat
     8581 tccatgagtc gccaagacca aggtctgttg tttatcttct ttgtcccaaa aagaaaatat
     8641 tcggaaatgt gttttggtga ataatgttct aaactcccaa atttctcctt tcagtttctt
     8701 aaataaatca ttatcatttt taatttttgc tttgtcaata ttgaaaatga ttttatctcg
     8761 acttttttca tcaagttgta ataaaaactc ccttgcttcg gtcaaaaaaa tcacttcaaa
     8821 tttattcatt taatctttca ttgtttccat acaaagatac aaaatttttc taatgaaaaa
     8881 atatttcgac aaattttatt tcgaagatga gttttggtgc tatttctgcc aacggtttgg
     8941 gtattgccga aggtgggtat ttgtagtact aagtccaatc gaagaacgaa tgttgagcct
     9001 tgcacaaatg tccaatcgaa gctattcagc ccggcttttg gcaataccat gttagcagta
     9061 gtggttcttg gttgtccgtc aattatatgg tcaatagttg tctccattgt ttacaaatat
     9121 tgtcaaagtc aatgcctgtg tttgcagggc ttgtgcttgg tagagaaata tatttcaaat
     9181 cactttgtct gtcgaaatac ttgtcgaaaa gtgcttggga ttttgccccg ttgaaaccga
     9241 taatttttaa attcttgtgt ctggcgataa agctgtccaa gtcgtttggt tcttcgtctt
     9301 caattgcact gtcaaggcta ccttttctgt ttgctttatg agctacgtcc caaattccaa
     9361 tttttgattt tagtaaaagt gcttttttgt ccgtgtaggt cagaggtaag tcgttgtctg
     9421 taattgtcga aatgattttc caaaatctgt ttcttgcatg tccgtaatac tcgccaagtg
     9481 caagagattt gtcgccaggc attgttccta aaatcaaaat ggtcgtgtcc gagtttgaaa
     9541 ttgggtcaaa cgatgttttg gtatttgtcg aggttgcttt tacaatttgt ttaactgtct
     9601 taggttgtga agttgtcgca ggttttactt tagtaactgc ttgtctgatt agggaaacag
     9661 tcgagccgtc acggtcaaaa atatttgcat agtttctcgt cctgccgtga atttgaaatg
     9721 cctgaattgc cgagccgtcc tttttctgat accaaccatt tttattcaac tcgtcagcaa
     9781 tttgctgggt cgtcataggt cgtccagttt gtcgtaaaag tttttctatt gcttcgtgta
     9841 gtgtcatatt tttctattcg ttgacggtcg tcctaccatt ggctataacg ttttgcggct
     9901 ttgcgaagaa gcggatttcg gagctcaaaa ctgtcaacat agcacaaaa
//
LOCUS       DAOQCW010000096         9867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_2606_length_9867_cov_5.481757, whole genome shotgun sequence.
ACCESSION   DAOQCW010000096 DAOQCW010000000
VERSION     DAOQCW010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9867
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2606_length_9867_cov_5.481757"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PLS16_08805"
     CDS             <1..249
                     /locus_tag="PLS16_08805"
                     /inference="COORDINATES: protein motif:HMM:NF025027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HNJ61220.1"
                     /translation="KNLVKKGLPPKVQEALDSVRVIGNESVHPGELNINDNREIAAQL
                     FKLVNFIATKMISEPKEIDEIYNSLPEKKLKGIEERDK"
     gene            652..999
                     /locus_tag="PLS16_08810"
     CDS             652..999
                     /locus_tag="PLS16_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNJ61221.1"
                     /translation="MNEKQKYRTITFYKDYFQTFFDKQNKKVKAKIVWTFDLVEDLER
                     VPETYLKHIENTDGLYEIRVQLGSDIFRIFCFFAKGQLVVLTNGFQKKTQKTPKKEIE
                     MAIKIKAEYENEK"
     gene            989..1288
                     /locus_tag="PLS16_08815"
     CDS             989..1288
                     /locus_tag="PLS16_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNJ61222.1"
                     /translation="MKNKNLMTLEDFKEKNYGKRGTKKRDELEAGYENFKIGVMLQQA
                     RLEKGMTQEELADRVGTTKSYISKIENNIKEARISTLQKIVELGFGGHLDLSIRL"
     gene            1470..1973
                     /locus_tag="PLS16_08820"
     CDS             1470..1973
                     /locus_tag="PLS16_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61223.1"
                     /translation="MKEYQLEKSIRTEKDFEQMGWHDCNIYGLIFHNDNTDNFTTNLI
                     FDIDYIFKWVHPTPPKQNFSFWVSPCTLKFEDTFALTIDIDRCGGMTDMLEVADLYLV
                     DKTEQETNKWIYEWAIELQEGKITFKSTGFKQIVRQKPILTESQILTLDERDGISFNQ
                     NPFDDRN"
     gene            2640..3194
                     /locus_tag="PLS16_08825"
     CDS             2640..3194
                     /locus_tag="PLS16_08825"
                     /inference="COORDINATES: protein motif:HMM:NF012344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61224.1"
                     /translation="MKVQLFVLILFFSSASVIALIAYYVFRSSQPTTTNKESVKDPIK
                     KRGIFLFIWFFILAVIFSVTLPKSPYYIFTDETPNQVVYVAARQFFFMLANEPIEPNV
                     PKMEKIEVPVNKTVEFRVTSFDVNHGFAIYNERSELVTQTQAMPGYVNKLRWKFKEPG
                     NYKIFCLEYCGAGHQLMQSSFVVK"
     gene            3202..4647
                     /locus_tag="PLS16_08830"
     CDS             3202..4647
                     /locus_tag="PLS16_08830"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNJ61225.1"
                     /translation="MTTLSLKKTTSAWIITILVLFIIAISFGITMRFNQGRDINLNPD
                     TFYSIMTVHGVTMIGIWSCAGMMAVNYLLSRYVKTSVKLNVFAYAFTVVGVLMIWAST
                     LIGKFHAGWTFLYPLPFRVAWAKWGTPLFLWGLTLLAVVWLVWAIGMVAQILRNYSLS
                     ESLAFQHLSRTKTPAKETPPFILISFVSLIGIIICLVTAAILMALLFAEYYSNGGFVN
                     DPLLVKNLTYFFGHTIANEALYLGLATLYEIMPEVSGRPKFKTTWYVALGWQLSLFFI
                     LTAFFHHLYMDFVQPISFQIIGQMASYLASIPSAVVTVLSVIFLVFGNKIKWNIVNGL
                     FFLGVTGWIIGGIGALIDATISNNIVLHNTLWVPAHFHTYNAMGNVLFSLAFFFWVGN
                     EFVDSKDYSKFNKLLLALLIIGGFGFVIMFYIGGANSIPRRYSVYPKELNLGDILATL
                     GGAFASVYLLAILWIFIDLMKKCIKVFSSAR"
     gene            4653..5375
                     /locus_tag="PLS16_08835"
     CDS             4653..5375
                     /locus_tag="PLS16_08835"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNJ61226.1"
                     /translation="MSNYISFAQESNYNYDEEKDIYKVISNVTIETEQGNQKLSTIYE
                     TSPTILVLIYTRCYGICNPLISNLSEQIQQLDIKKDFRVLVVSFDAADRLSDMERYAQ
                     QFDLQNDARWIFVTTKQIKPLIESVNFNPKWDSTIQQFDHNALLVGLNTNGYITKKII
                     GIRGSNELQAMLKEINNEFIVSYPLPRKNMLFSCFTYNPTTGKSELSIGLLVMLAPAL
                     ITFIIVLSLIKLGKKQRKTLVK"
     gene            complement(5376..6671)
                     /locus_tag="PLS16_08840"
     CDS             complement(5376..6671)
                     /locus_tag="PLS16_08840"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNJ61227.1"
                     /translation="MNYLNIFLFTAFSLLGSVLKADDCNNGRYQKPVFSRVQVINNIY
                     YATKIQSDNKPIHLRYDIYQPAGDTLSERPIMLLIHGGAYLKLLDQSSPDIVLMCNYF
                     AKLGYVAVSINYRQEPNPLSLLSGEKMVKAVGRALIDTKEAVDHLMLTYQNGNPYRID
                     TSRAFIGGVSAGGVSSLFITFLDSLSMLPPQFQEWIIESNGIEADSILKHKFDLVKPK
                     AAISISGAISDLSWIRNVGIDLFLNHGSADELVPYRTGKPFGLPDLPTLSGGKDIYPL
                     AVQAGIRTFFEDWEGRGHVPFMNLDFGSIITLQLINQPILDSTLHNITRFIYPLLGCE
                     EDKATSIRQNKIADLKLFPNPSNGIFNIDMPKRNNNNEGYLQLFDLNGKLYVERKINP
                     QESVLHFDENLSSGQYFIKLSFKEKNIIDYYTASFSVVK"
     gene            6855..8867
                     /gene="uvrB"
                     /locus_tag="PLS16_08845"
     CDS             6855..8867
                     /gene="uvrB"
                     /locus_tag="PLS16_08845"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNJ61228.1"
                     /translation="MEFKLYSHFSPAGDQPSAIDALTNGITKGEKHQTLLGVTGSGKT
                     FTIANVVEKIQKPTLVLTHNKTLVAQLYAEFKTFFPDNKVEYFVSYYDYYQPEAYVPT
                     TDTYIEKDLAINAEIEKLRLSTTSSLMSGRNDVLVVASVSCIYGLGNPAEYKNQIIRL
                     KIGQTIARNALLFDLVSILYARTTDDFQRGNFRVIGDTVEVFVAYDDYAYRITFWGND
                     IEALEVFDPKTKHKIETRTEIAIYPANIYIAPKEMLPTIISEIQDELYHQIKYYESLG
                     RFPEAKRLEEKTNFDLEMIRELGYCSGIENYSRYFDRRKAGDRPFCLLDYFPDDFILV
                     IDESHVTLPQVSGMSGGDRSRKQNLVEYGFRLPSALDNRPLNFYEFESLINQVVYVSA
                     TPGDYELRQTEGLFVEQVVRPTGLLDPPIEIRPSSNQIDDLIEEIRKRKVKDERILVT
                     TLTKRMAEELAKYLTKLKIACKYIHSDVDTMERVEIIQELRLGEFDVLIGVNLLREGL
                     DIPEVSLVAILDADKEGFLRNTRSLTQTAGRAARNANGLVIFYADKITQSMQQTINET
                     NRRREKQVAYNIKHNITPKTIFKSKEDIIQSASILDIRGDKNKSIQTKETLLIVAEEE
                     KAFMSKDAIKKAIEQTKKAMQKAAQKMDFMEAARLRDEMYKLESML"
     gene            complement(8897..9805)
                     /locus_tag="PLS16_08850"
     CDS             complement(8897..9805)
                     /locus_tag="PLS16_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61229.1"
                     /translation="MKNSSFKTKNLIVSFLVFIVIILPITSCKKEKSSPKVIDVYVVG
                     YENIGTKDVARIWKNGVTTSLTDGINGADANSVFVQGNDVYVAGYESNGAKYVAKIWK
                     NGVATSLTDGTQHSYANSVFVQGNDVYVAGYEYNGTKYIAKVWKNSLATNLTDGTLDA
                     YVQSIFVQGNDVYVAGYEYNGIMFIAKFWKNGVATSLTDGTHNAYAESVFVQGNDIYV
                     VGNEDFGTNRIAKIWRNVVATNLSDGTVYTIANSVFVQGNDVYVTGFEYNGTTNIAKI
                     WKNGVVTNLTDATQFASAKSIFVVER"
BASE COUNT         3544 a         1587 c         1562 g         3174 t
ORIGIN      
        1 aaaaaccttg ttaaaaaagg attaccccca aaagttcaag aagctttaga tagtgtacgt
       61 gtaattggaa atgaatcagt tcatccagga gaattaaata ttaatgataa tagagaaatt
      121 gcagcccaac tctttaaact agtaaatttc attgctacca aaatgatttc tgaaccaaaa
      181 gaaatcgatg aaatatataa ttctcttcca gaaaaaaaac ttaaaggaat tgaagaaaga
      241 gacaaataga actacaccca acagcaccta cccaaaagtg gcatttcagt ggttaaatca
      301 agctttgagt ttctatcaaa gtttgtgctt ggttgacagt gaagtgctcc gaaatcgcca
      361 actgcggcaa cacgcaaaac gatgtggcga aatccgctgc gctcctttcg ccacatcgtg
      421 ccggcggctt acaccgcctg atgcactcca gtcgcttcgc tgactgtcgc acatcaggcg
      481 gttttgctaa atttcaactt cgagcaaagc tctagttgaa acttcgcaaa gccgccggca
      541 cgttatgggc aagtttaacc gaactcttcg ccaaaaattt cacatactac gataagcaaa
      601 aaacacacaa aacatagttg cactaaagag aaacttttgt atctttgcaa agtgaatgaa
      661 aaacagaagt atagaacaat aactttttac aaagactatt ttcaaacttt ttttgacaaa
      721 caaaacaaga aagtaaaagc gaaaatagtt tggacatttg atttggtaga agatttagaa
      781 agagttccag aaacgtatct aaagcatata gaaaatacgg atggacttta cgaaatccga
      841 gtacaattag gaagtgatat ttttcgcatt ttctgttttt tcgccaaagg acaattagtg
      901 gttttgacta atggctttca gaagaaaact caaaagacac cgaaaaagga aattgaaatg
      961 gcaattaaaa taaaagcaga atatgaaaat gaaaaataaa aacttgatga cactggaaga
     1021 ttttaaagaa aaaaactacg gcaaacgtgg aacgaaaaaa cgtgatgaat tggaagctgg
     1081 ttacgaaaac tttaaaatcg gagtaatgct tcaacaagca agacttgaaa aaggtatgac
     1141 acaggaagaa cttgccgaca gagttggtac gacaaaatca tatatttcaa aaattgagaa
     1201 caacataaaa gaagctcgaa tttcgacact tcaaaaaata gttgaacttg gttttggagg
     1261 acatttagat ctatcaataa gattataatg gaaagaaaac ctgcccataa caaacaaatt
     1321 ggcaatagag ccggtgaagt gctactattg agcttttgtg caaggttgaa tggtagtaat
     1381 tctattcaac atttgtgcta atagtccgct ccctcgcaaa gctgcaaacc gttagctgtc
     1441 attttaaccc gaaattcacg acaaaaacaa tgaaagaata ccaacttgaa aaaagcatta
     1501 ggactgaaaa agattttgaa caaatgggtt ggcacgattg taatatttat ggtttaatct
     1561 ttcataacga caatacagac aactttacaa caaacttaat tttcgatata gattatattt
     1621 tcaaatgggt acaccctact ccacctaaac aaaatttctc attttgggtt tcgccttgta
     1681 cactaaaatt tgaagatact tttgcattga ctattgacat tgacagatgt ggaggaatga
     1741 ctgatatgtt agaagttgca gacttatact tagttgacaa aactgagcaa gagaccaata
     1801 aatggattta tgaatgggca atagaattac aagagggaaa aataactttt aagtcaaccg
     1861 gatttaagca aattgtaaga caaaaaccta tattgaccga atctcaaatc ttgactttag
     1921 acgaaagaga cggaatttca tttaatcaaa atccatttga tgacagaaat taaaaaatgt
     1981 gacgaatgtg aaagtgaata tttcgctaat tcatccaaaa tgttaaacct ttgtcctgaa
     2041 tgttctcata ttttatatgg ttatcaaaat tgcaatcaca tttttaaaga aaacagatgc
     2101 ataaagtgtt attggaacgg aaacgtaacc aaatatttaa aacaactaaa gcaaaaaaat
     2161 aaaaacgaca gctaaacatt atgacgaata gaagaaggcc ctaaaaagta aagctctgag
     2221 ccaatgctca gtgtttttta tctcacttaa agcgagatag tacttcgaca aactcagcat
     2281 ctcattcagc gacacaattt aagttttcta ataggtggtt tagtagcatt tatctacgct
     2341 attttgtagg aattacaaaa tttcatcttc ttcgaattgg tagggaaaaa ttttcttcta
     2401 aactttaaat ttccaccaac aacttttaag tacgttacac tattttacca attccaaaat
     2461 tcatattaac gcaaatatga ccataattat aagaaattta ttaaattttt attaaaatag
     2521 gatatttata tctttttatc tgattattat tatatttgtc taagaaaaca agaagttctt
     2581 tgccggaaac gacaatattt tagtttgatt tttattaact ctactataat ttttgaagta
     2641 tgaaagtaca attattcgta ttgatattat tttttagctc tgcatctgtc atagcattaa
     2701 tagcatacta tgtttttagg tcatcgcaac caacaacaac caacaaagaa tctgtaaaag
     2761 atcctataaa aaaacgaggt atatttctat ttatttggtt ttttattcta gccgttattt
     2821 tttcagttac tttacccaaa tctccttatt atatttttac agatgaaaca cccaatcagg
     2881 ttgtttatgt tgcagccaga cagtttttct ttatgttggc taacgaacct atcgaaccta
     2941 atgttccaaa aatggaaaaa attgaagttc cagtaaataa aaccgtagaa tttagggtta
     3001 caagttttga tgtgaatcat ggatttgcaa tttataatga acgctctgaa ttggtaacac
     3061 aaacacaagc tatgcctggc tatgtcaaca aacttcgttg gaaatttaaa gaacctggca
     3121 actataaaat attttgttta gaatattgcg gtgctggaca ccaactaatg caatcatcat
     3181 tcgtcgttaa ataaaaaagc cgtgacaaca ttatcattaa aaaaaacaac ctccgcttgg
     3241 attattacaa ttttagtatt atttatcatt gctatatcgt ttggaattac aatgcgattt
     3301 aaccaaggaa gagatataaa tttgaatcct gataccttct attctattat gacagttcac
     3361 ggcgtaacga tgattggtat atggtcatgt gctggaatga tggctgtaaa ttatttactg
     3421 tctagatatg taaaaacatc agtaaaattg aatgtcttcg cctatgcttt tacagttgtt
     3481 ggcgtattga tgatttgggc atcaacctta ataggaaaat tccatgctgg atggactttt
     3541 ctatatccat taccattccg agtagcttgg gcaaaatggg gaacaccttt atttttatgg
     3601 ggattgacgc ttttagcagt cgtttggttg gtatgggcaa ttggtatggt tgcacaaatt
     3661 ttaagaaatt atagtctttc tgaatcccta gcatttcagc atttatcaag aactaaaact
     3721 ccagctaaag aaacacctcc atttatacta atttcatttg tctccttaat tggaatcata
     3781 atatgtttgg taacagctgc tattttaatg gcattacttt ttgctgaata ttattcaaac
     3841 ggcggatttg tgaatgatcc tttgctcgta aaaaacctaa cctatttctt tggtcatacc
     3901 attgcgaacg aagctttata cttaggattg gctactttat atgagattat gcctgaagtt
     3961 agtggacgac caaagtttaa aactacttgg tatgtagctc taggttggca actatcatta
     4021 ttctttattt tgactgcatt tttccatcat ttatatatgg attttgtaca gccaattagt
     4081 tttcaaatta ttggacaaat ggcatcttat ctagcaagta ttccatcggc agttgttact
     4141 gtattaagtg tcatatttct tgtttttgga aataaaataa aatggaatat tgttaatggt
     4201 ttatttttcc ttggtgtaac gggttggatt atcggaggta ttggtgcgtt aattgatgca
     4261 accatatcaa acaatatagt tttgcacaat acattatggg tacctgcaca ctttcataca
     4321 tataacgcaa tgggcaatgt actgtttagt ctagcattct ttttttgggt tggtaatgaa
     4381 tttgtagatt ctaaagacta tagcaaattc aataaactac tattagcatt attaattatt
     4441 ggtggatttg gatttgtaat tatgttttat ataggtggag ctaattctat tccacgacga
     4501 tattctgttt atccaaagga attaaactta ggagacatat tagcaactct tggtggagct
     4561 tttgcaagtg tttatctatt agcaatttta tggatattta tagatttaat gaaaaaatgt
     4621 ataaaagtat tttcatctgc acgatagtct tattgagtaa ttatatatca tttgctcaag
     4681 aaagtaatta caattatgat gaggaaaaag atatttataa agtaatatct aatgtaacga
     4741 ttgaaactga acaagggaat caaaaattat ctacaatata tgaaacttcg ccaaccatcc
     4801 ttgtgcttat ttatacaaga tgctatggta tttgtaatcc actcatcagt aatttatcag
     4861 agcaaattca gcaactagat atcaagaaag attttagagt tttagtcgta agttttgatg
     4921 ctgctgatag gctgagtgat atggaacggt acgctcaaca atttgactta caaaatgatg
     4981 ctcgttggat ttttgtaacc acaaagcaaa ttaagccatt aatagaatct gtaaatttca
     5041 atccaaaatg ggatagtact atacaacaat ttgaccataa tgcattatta gttggtttaa
     5101 ataccaatgg ttatatcacc aaaaaaatta ttggcataag aggctcaaat gaattacaag
     5161 caatgttaaa agagataaat aatgagttta ttgtatccta tccattgcca agaaaaaata
     5221 tgctattttc atgttttact tacaatccaa caactggaaa aagtgaactc tctatcggtt
     5281 tattagtgat gttagcacca gctttaatta cattcattat tgtactaagc ttaattaagt
     5341 taggtaaaaa gcaaagaaaa acacttgtaa agtaactatt taacaaccga aaaacttgcg
     5401 gtataataat ctattatatt tttttccttg aaagacaatt ttataaaata ctgaccactt
     5461 gataaatttt catcgaaatg caaaacagat tcttgaggat taatttttcg ttctacatac
     5521 aatttaccat tcaaatcaaa caattgtaaa taaccttcat tattattatt tctctttggc
     5581 atatcaatat taaatatacc attacttgga ttgggaaata attttaaatc tgcaatttta
     5641 ttttgtctga tacttgttgc tttatcttct tcacaaccta ataatggata aataaatctt
     5701 gtaatattat gcaaagtaga atccaatata ggttgattaa tcaattgtaa agtgatgata
     5761 cttccaaaat ctaaattcat aaaaggaaca tgacctcgtc cttcccaatc ttcaaaaaaa
     5821 gtccttatac ctgcttgaac tgccaaagga taaatatctt ttcctccact caaagttggt
     5881 aaatctggta gcccaaaagg ttttcccgtt ctataaggca ctaactcatc tgctgaaccg
     5941 tgattcaaaa ataaatcaat cccaacattt cgaatccaac ttaaatctga aattgcacct
     6001 gaaatagaaa ttgcagcttt tggttttact aaatcaaatt tatgttttaa aattgaatct
     6061 gcttcaattc cattagactc aataatccat tcttgaaatt gaggtggcaa catagaaagg
     6121 ctatctaaaa atgtaataaa caaggaagac acgccaccag cagaaacgcc gccgatgaat
     6181 gccctagaag tatctattct ataaggattt ccattttgat aagttagcat taaatgatcc
     6241 acagcttctt ttgtatctat taatgcacga ccaacagctt ttaccatttt ttcgccagat
     6301 aataaagaaa gtggattggg ttcctgtctg taatttatag acactgcaac atagcccaat
     6361 tttgcaaaat aattacacat tagaacaata tctggactac tttggtctaa taatttcaaa
     6421 tatgcaccac catgaataag taacataatt ggtctttctg aaagcgtatc tcctgcaggt
     6481 tgatatatat catatcttaa atgaatcggt ttattatcgc tttgtatttt agtcgcataa
     6541 taaatattat taataacctg aactcttgaa aaaacaggct tctgatagcg accattatta
     6601 caatcatcag cttttaaaac agaccctaat aaggaaaatg ctgtaaataa aaaaatgttt
     6661 aaataattca tggagaattt ttgattaaac aaaataagta aaaagaataa caatctattt
     6721 atatcctatt aaaagctcgt tcaaagcata taaatcttaa cttatgttaa ttttaaattc
     6781 aaactaaaaa cagttatcaa aacaacaatt gctttgattg gataaacata ttctatattt
     6841 ttacaataca atttatggaa ttcaagttat atagtcattt ttctccagct ggcgaccaac
     6901 caagtgctat tgatgcttta acgaatggaa ttacaaaagg cgaaaaacat caaactttgt
     6961 tgggtgttac tggctctgga aagactttta ctattgctaa tgtagttgaa aaaatacaaa
     7021 aaccaacttt ggttttaaca cataataaaa cgttggttgc acaattatat gctgagttca
     7081 aaactttctt tcctgataat aaagtggagt attttgtttc gtattatgat tattatcaac
     7141 cagaagctta tgtgccaact actgatactt acatagaaaa agacttagct attaatgctg
     7201 aaattgagaa attgagatta agtacaacct catctttaat gagtggcaga aatgatgtat
     7261 tggtggttgc atcggtttct tgtatttatg gtttaggtaa cccagcggaa tataaaaacc
     7321 aaataatacg tttaaaaata ggacaaacta ttgctagaaa tgcactattg tttgatttag
     7381 tttctatatt atatgcaaga actactgatg attttcaacg aggaaatttc agggtaattg
     7441 gagatactgt tgaggttttt gttgcttatg atgattatgc ttatcgaatt acattttggg
     7501 gaaatgatat cgaagcctta gaagtttttg acccaaaaac gaaacacaaa atagaaactc
     7561 ggacagaaat agctatttat ccagctaata tctatatcgc accaaaggaa atgttaccta
     7621 ctatcatttc tgaaatacaa gatgaattat accatcaaat aaaatattac gaatccttgg
     7681 gtcgttttcc agaagccaaa agattagaag aaaaaaccaa ttttgatttg gaaatgatac
     7741 gagaattagg atattgttct ggtattgaaa actactctcg atattttgac agaagaaaag
     7801 caggcgatag gcctttctgt ttattagatt attttccaga tgattttatt ctagtcatcg
     7861 atgaaagtca tgttacttta ccacaagtaa gtggtatgag cggtggcgat agaagtcgca
     7921 aacaaaattt agttgaatat ggatttcgat tgccatcagc gttagacaat agacctttaa
     7981 atttttacga atttgaaagt ttaatcaatc aggttgttta tgtttcagcc acaccaggcg
     8041 attatgaatt acgacaaact gaaggtttgt ttgtagagca agttgtgcgt ccaactggtt
     8101 tattagaccc accgatagaa atcagaccaa gctccaacca aattgatgac ttaatagaag
     8161 aaattagaaa acgaaaagta aaagatgagc gtattttagt tacaaccctg actaaaagaa
     8221 tggctgaaga attagctaaa tatctaacga aattaaaaat tgcttgtaaa tatattcaca
     8281 gcgatgtgga cactatggag cgtgtcgaaa ttattcagga attgcgttta ggtgaatttg
     8341 atgttttaat cggcgtaaat ttattgagag aaggtttaga tattccagaa gtttcgcttg
     8401 tagccatttt agatgcagat aaagaaggat ttttgcgtaa tactcgctca cttactcaaa
     8461 cagcaggacg tgctgcaaga aatgccaatg gtttagtaat attttatgca gataaaataa
     8521 cgcaaagtat gcagcaaacc attaatgaaa ccaacagaag aagggaaaaa caagttgctt
     8581 acaatatcaa acacaatatt acgccaaaaa ctatttttaa atccaaagaa gatattatcc
     8641 aaagtgccag tattttagat attagaggcg acaaaaacaa atctatccaa acgaaagaaa
     8701 ccttactaat tgttgctgaa gaagaaaaag catttatgag caaagatgct attaaaaagg
     8761 ctatagaaca aaccaaaaaa gcaatgcaaa aagcagctca aaaaatggat tttatggaag
     8821 cagctaggct tcgtgatgaa atgtataagc tagaaagtat gctttaataa tttcaaaata
     8881 gtctatcaca aaaatcctat ctttctacga caaaaattga ttttgctgaa gcaaattgag
     8941 tagcatcagt aagattcgtt acgacaccat ttttccatat tttggctata ttcgttgttc
     9001 cattgtactc aaatcctgtc acatacacat cattgccttg tacaaatact gagttagcaa
     9061 tagtataaac tgttccatct gaaagatttg tagctactac atttctccat atttttgcta
     9121 tcctatttgt tccaaaatct tcatttccta ctacataaat atcattccct tgtacaaaaa
     9181 cagattcggc ataagcatta tgagtgccat cagtaaggct cgtagctact ccatttttcc
     9241 aaaatttagc tataaacatt attccattat actcatatcc tgccacatat acatcattac
     9301 cttgtacaaa aatagattga acataagcat caagagtacc atcagtaaga ttcgtagcta
     9361 aactgttttt ccaaactttt gcgatatact ttgtaccatt atactcatat cctgccacat
     9421 acacatcatt accttgaaca aatacagaat tagcataaga atgttgagta ccatcagtaa
     9481 gactagtagc tacaccgttt ttccatattt tggcaacata cttagctcca ttactctcat
     9541 atccagccac atatacatca ttaccttgta caaatactga atttgcatct gcaccattga
     9601 ttccatcagt aagactcgta gttacaccat ttttccatat tctggcgaca tccttagttc
     9661 caatattttc atatccaact acataaacat ctataacttt aggagaagat ttttcttttt
     9721 tacaagatgt tatgggtagt atgattacaa taaatactaa aaaacttaca atcaaatttt
     9781 tagttttaaa agatgaattt ttcataatat gaattttatt atttaaagta aaatctaatt
     9841 tcttaaataa tcaatactaa aaacata
//
LOCUS       DAOQCW010000097         9723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_2650_length_9723_cov_5.085954, whole genome shotgun sequence.
ACCESSION   DAOQCW010000097 DAOQCW010000000
VERSION     DAOQCW010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9723
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2650_length_9723_cov_5.085954"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="PLS16_08855"
     CDS             <1..953
                     /locus_tag="PLS16_08855"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HNJ61230.1"
                     /translation="NPKLPMQRGKYEGFYEDDLKSSKFNGRLQYEKAQEILKGEYQQI
                     KGEEVDAVIQYLDFGAIDILPEFNNGQSGAVTSPSAMGIPFLVGSIMDGPGMHPMIGK
                     GASLVAEFVKLQDLNKAKNMNDKDAEAIHRKYKAQGKKHLAIEAGAKRLLGVRDLRDF
                     FIPGFADPTIKYLKHFARLGLFDEHAWTPQILPIQIFRVGSIAFIAFPFEITTVASWR
                     LKDTLKEKLKSSGIEEIILCPYSNSFNGYITTFEEYQVQAYEGGHCVFGQWSLNALQQ
                     VSSNLASQLLKNNKDRDIPLVEDKPISKDYLERFAYKRKP"
     gene            1062..2054
                     /locus_tag="PLS16_08860"
     CDS             1062..2054
                     /locus_tag="PLS16_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61231.1"
                     /translation="MKQKMNLIALLLILMIGNVFAANFQVTVNLKNGDIISGKSTFGN
                     ILLKSKYGDLSIPSEEISNITFGVYTDWSKAAQVNQDLKKLQMSSDNEAKVIYNKLLE
                     MGAPILSIVNNYTLSDIYEIGDANTYSIDNLLIELYEKAGLSIGDALEDVVEFGVNAN
                     KMEGSLVLAEIKITNNYGAFVFKKDDISSIKLSVIDEKVYGNNTFKLKANVNISGNDA
                     NNAWLDTGIKVSEGQEIKITANGKIKLYSLSGGIYSPSGYASGPTDASYDESAEIPYG
                     SVVYKIGTADSIHKVVSNEIFTAPNKGTLYLCIYETVFNDTNVGSYIVKVDTKK"
     gene            2126..2794
                     /locus_tag="PLS16_08865"
     CDS             2126..2794
                     /locus_tag="PLS16_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ61232.1"
                     /translation="MKKRIFLLPGFGEDAFSFDEIRPFLKDFDVIDVDYRKSLRKFIF
                     PFINVKNFAKQLIKDNHINADDKLIGHSMGGYFSFQIREIQDNPICMIASFNDPAKIF
                     HTFPDYPRITQLAALTGFVKTTYLKKMLLDKIKDSNIRNIQSAVMDNFDTFSNIELSL
                     MAEMNYAPKIKSLKPNPLRLHSSKDRVVMPPDEPFVEIGGGHFCQNLYPKDTIDAMKD
                     FLLK"
     gene            2863..3300
                     /locus_tag="PLS16_08870"
     CDS             2863..3300
                     /locus_tag="PLS16_08870"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNJ61233.1"
                     /translation="MKGLEFLKQLKFPFPIKQNVYWGEMDAFGHINNVIYFRYFETGR
                     INFFNQTGFWKDLDNETVRIVVVKLDCNFVQEIIYPKEIGIAVAIKEIGNSSVKVHHI
                     VYDANDTNTIFAHGEGVIVGTDPQTGKSKPWSSLLKEKLSQFL"
     gene            3310..4515
                     /locus_tag="PLS16_08875"
     CDS             3310..4515
                     /locus_tag="PLS16_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HNJ61234.1"
                     /translation="MKDYSRASLALHEEINGKITVELKTPLENKNDLTLCYSPGVAEP
                     VRQIANDEERVWDLTIKGNTVAIISDGSSILGLGNLGAEASIPVMEGKAMLFKRFGGL
                     NAFPICLATQDTEEIIRTIRNIAPVFGGINLEDISSPRCFEIEDRLQTLGIPVFHDDQ
                     HGTAIVALAALFNACKLFKKNFGDLKVVINGAGAAGLAITKLISCDDLETDICTPVRE
                     IIMCDTKGIIHKDRPDLNDFKRDILTYTNKNHVKGTLKDAIKDADVFIGVSQGSILSS
                     EDITTMNKDAIILALANPTPEISPELAYKGGAGVVGTGRSDLPNQVNNVLAFPGIFKG
                     ALAVRARRITNRMKIAAAYAIADCVVNLERDCIIPNALDLGVADRVAEAVMKAYLEDK
                     NLEAPKHEL"
     gene            4636..5328
                     /locus_tag="PLS16_08880"
     CDS             4636..5328
                     /locus_tag="PLS16_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNJ61235.1"
                     /translation="MKNKITTIAFDADDTLWVNEPYFQEAEKQFCALLENYLPQHTIS
                     QELFKTEMKNLHLYGYGIKGFLLCMIETANSVSNRTVSLNVIDKIIEIGHDLLQKPIE
                     LLQGVQETLEALNGDYKLVVATKGDLLDQERKLKNSGLEHYFHHIEIMSEKKESDYQK
                     LFKHLDCQSENFLMLGNSIKSDIISILELGGNAAHIPYHVTWTHEQQEVNLKHNNFIQ
                     LKSINEILNYLK"
     gene            5331..5861
                     /locus_tag="PLS16_08885"
     CDS             5331..5861
                     /locus_tag="PLS16_08885"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNJ61236.1"
                     /translation="MEINIRQEALVDYPIVFDLIEQAFRDEIISDKKEQFLVERLRKS
                     DGFIPKLSLVAEANQKIVGYILLTKIIIETNTESVTSLALAPVAVLPTFQKKGIGGRL
                     IEEAHHIAKGLGYTSVILLGHETYYPKFGYKLLKEFNITLPFVAPKENCMAIELIPDA
                     LKNKEGKVIYPKEFFE"
     gene            5876..6502
                     /locus_tag="PLS16_08890"
     CDS             5876..6502
                     /locus_tag="PLS16_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /protein_id="HNJ61237.1"
                     /translation="MKKLIDLDNWNRKEHFLFFSKFDEPFFGVTVKVDCTIAYQKAKE
                     KGVSFFLYYLYRALKAANEVENFRYRIIENQPYDYATTNASPTINRPNGTFGFAYINY
                     FENENKFYEYALQEIENVKKTSSLMPSISGENVIHFSAIPWIDFTSISHARNFSHPDS
                     CPKISFGKMTENNGQKEMSVSIHVHHALMDGYHVGLFIEKFQSLLSEA"
     gene            complement(6564..7427)
                     /locus_tag="PLS16_08895"
     CDS             complement(6564..7427)
                     /locus_tag="PLS16_08895"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ61238.1"
                     /translation="MLYDITHKQVKVNDIFLHVAQQGNGKKLVVLLHGWPEFWYTWRY
                     QMPELAKEYTVIAPDLRGFNKSDKPKGIQNYKADVVASDVAELIKQMGFKKAYVVGHD
                     WGGAIAWTFASIYPNMLEKLAIINCPHPKEMLKMIKSNPTQLLKSWYMFMHQLPLIPE
                     FILGNTLPQFFKLFFRGWMHHPEHFTDKDLQAFVRAYKEPGALTGSINYYRAMMQTKP
                     KMSVFKQKVTVPTLLIWGEDDKALSKELTYNTQAYVDNTLDIKYIKNCSHWAQNDCPK
                     EVTQYLVDFFK"
     gene            complement(7432..8994)
                     /locus_tag="PLS16_08900"
     CDS             complement(7432..8994)
                     /locus_tag="PLS16_08900"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HNJ61239.1"
                     /translation="MKKLHKNTLIFLTLLFNVNNIFSQHILHQPIIGAITENSAKILF
                     FSDTLIDYTLTISNQEGIMSIEHHTEDSIFFCNKLTISELMPNQTCYYTLKTKNNTEE
                     FRGTFKTAPKKNSTEKFSFVFGSCTEQSQDFGIFKTIEKQKPNFFIHLGDWLYNKYNA
                     TTDLSEQIELFHLRDKFIARYKLLGNFLKNTPIDYIFDDEDGIYDDFSKSSFTVIEAN
                     NKSTQVKEIPYPDSLKTILKKSLAEFFPSYDDVNNQDSYHKFSYGNADFFVLDNRSTR
                     TPNTEIFTTNKKGKLKYSNPNEHEILDSTQMKWLLDGLKKSEAKWKFIVSGVTFNKSY
                     KKVFNICMRLQNRILPNGKNGAYIAASLSAMWFAYPNSQQKLINFCKDNEIKNIIILS
                     GDAHSSAIDDGKNAGFPEIMAGGLAQVNSHIASIIYNNFKLNLWNQGGQGIQNKNFNH
                     AYGKVTVNQDSSVILEVIDDKDSTIAKHQIKTDYLPNTFKLKKQVRINKLFTFRKKIR
                     IGFREILGKNKF"
     gene            9028..>9723
                     /locus_tag="PLS16_08905"
     CDS             9028..>9723
                     /locus_tag="PLS16_08905"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNJ61240.1"
                     /translation="MFSDKIIFIVGNSRSGTTMMLRIFNNHDQLMVLNELHFFEELWS
                     PIDKGKSISRADAIFLMQKLLLIQRKGYKVGFDDFSIFYDEAKQVLDKFEDKDLIPEN
                     IFYQFAKREVALNGKSIVCEKTPQNVFYLKEIFELFPNAKVINMVRDPRGVMLSQKNK
                     WNRRNLGGWYITKKEARRLRINYHPITLTTLWNAAINAVKKNEDDQRILTVIFEELVN
                     QPEQEIKKICKHID"
BASE COUNT         3423 a         1300 c         1649 g         3351 t
ORIGIN      
        1 acaatcccaa attaccgatg caaagaggaa aatatgaagg attttatgaa gatgatttaa
       61 aaagttctaa gtttaatgga cgattacaat atgaaaaggc acaagaaatt ttgaaaggag
      121 agtatcaaca aataaaagga gaggaagtcg atgcagttat tcaatattta gactttggag
      181 caattgatat tttgccagaa tttaataatg gacaatcagg agcagttact tcgccatctg
      241 ccatgggtat tccattttta gtaggctcaa ttatggatgg accaggtatg cacccaatga
      301 taggaaaagg agcaagtttg gttgctgagt ttgtaaagtt acaagatttg aataaagcta
      361 aaaatatgaa tgataaagat gcagaagcta ttcatagaaa atataaagct caaggtaaaa
      421 aacatttagc aattgaagct ggagcaaagc gtttgctggg tgttcgtgat ttaagagatt
      481 tttttattcc aggttttgct gacccaacaa ttaaatatct taaacatttt gcaagattgg
      541 gtttgtttga tgaacatgct tggactccac aaatcttgcc catacaaatt tttagagtag
      601 gaagtattgc tttcattgct tttccatttg aaataaccac agtggcaagt tggaggttga
      661 aagacacact aaaagaaaaa ttgaaatcat ctggaattga agagattatt ctttgccctt
      721 atagtaattc atttaatgga tatattacta catttgagga atatcaagtg caagcttatg
      781 aaggtggaca ttgtgttttt ggtcaatgga gtttaaatgc tttacaacaa gtaagttcta
      841 atttagcctc gcaattacta aaaaacaata aagatagaga tattccgttg gtagaggata
      901 aaccaatatc taaagattat ttagaaagat ttgcttataa aaggaagcca taaatgaact
      961 gatttttctg tttttttatg ctctaaataa ctttttttat gatttatgaa tgcatattat
     1021 tataattagt ttttaaattt aaaatcaaaa taaataaaat tatgaaacag aaaatgaacc
     1081 taattgcact attattaata ttaatgatag gtaatgtatt tgcagcaaat ttccaagtaa
     1141 cagtgaatct taaaaatgga gatattatct cagggaaatc aacatttgga aacattttac
     1201 taaagtcaaa atatggagat ttaagcattc catctgaaga aatttcaaat attacttttg
     1261 gtgtttatac agattggtct aaagcagcac aagtcaatca agatttgaaa aaattgcaaa
     1321 tgtcaagcga taatgaggct aaagttatct ataacaaact tttagaaatg ggagctccta
     1381 ttctttctat tgtaaataat tatactttat cagatattta tgaaattgga gatgccaata
     1441 cttattccat tgacaattta ttaatagaac tctatgaaaa agcaggatta agtataggag
     1501 atgcattgga agatgtagtt gaatttggtg taaatgctaa taaaatggag ggaagtttag
     1561 tgttagctga aattaaaatt acaaataatt atggtgcatt tgtttttaaa aaagatgata
     1621 tttcttcgat aaagttatct gttattgatg aaaaagttta tggaaataat acttttaaat
     1681 tgaaagcaaa tgtaaatatt tctggaaatg atgcaaataa tgcttggtta gatacaggca
     1741 taaaagtaag tgaaggacaa gaaattaaaa ttactgcaaa tggtaaaatt aaattatata
     1801 gtttatctgg aggaatatat tctccgagtg gttatgcaag tggtccaact gatgcttctt
     1861 atgatgaaag tgccgaaatt ccttatggtt cagttgtata taaaattggt acagcagatt
     1921 caattcataa agttgttagt aacgaaattt ttactgcacc aaataaaggt actctatatt
     1981 tgtgtattta tgaaactgtt tttaatgata ccaatgttgg ttcatacatt gtaaaagtag
     2041 atactaaaaa ataactttag ataaaataaa tattttgaaa gccatttttg tttttaaaaa
     2101 tggctttttt gtttattttc agtcaatgaa aaagagaata ttcctattgc caggttttgg
     2161 agaagatgcg tttagttttg atgaaattag accatttttg aaagattttg atgttattga
     2221 tgtggattac agaaagtcat tacgcaaatt tatttttccg tttatcaatg ttaagaactt
     2281 tgcaaaacag ctcattaaag ataatcatat caatgcagat gataaattga taggtcattc
     2341 aatgggaggt tatttttctt ttcaaattag agagattcaa gataatccga tttgtatgat
     2401 cgcttcattt aacgaccctg caaagatttt tcatacattt cctgattatc ctagaattac
     2461 acaactagcg gcacttactg gttttgttaa aactacttat ttgaaaaaaa tgttgttgga
     2521 taaaattaag gactcaaata ttagaaatat acaaagtgca gtaatggata attttgatac
     2581 tttttcaaat attgaattga gtttaatggc agaaatgaat tatgcaccca aaataaaatc
     2641 tttaaagcct aatccattgc gattgcattc aagcaaagat agagtagtaa tgccacctga
     2701 tgagcctttt gttgaaattg gtggaggtca tttttgccaa aatctttatc ctaaagatac
     2761 catagatgct atgaaagatt ttctcttaaa ataaataact tttatataaa ctacatttag
     2821 ctgttagatt caaagtctta cactaactta gtaggcttaa atatgaaagg attagaattt
     2881 ctaaaacaat taaaattccc tttcccaata aaacaaaatg tttattgggg agaaatggat
     2941 gcttttgggc atatcaataa tgttatttat tttagatatt ttgaaactgg aaggattaat
     3001 ttttttaatc aaacaggttt ttggaaagat ttagataatg agacggtaag aattgttgtt
     3061 gtaaaattgg attgtaattt tgtccaagaa attatatatc caaaagaaat cggaattgct
     3121 gttgcgatta aagaaattgg aaattctagt gtgaaagtgc atcatattgt ttatgatgcc
     3181 aatgatacaa atactatttt tgctcacgga gagggtgtaa ttgttggcac agacccacaa
     3241 acaggtaaat ctaaaccttg gagttcatta ttaaaagaaa aattaagtca gtttttataa
     3301 gcacaatata tgaaagatta ttccagagca tcattagcat tacatgagga aataaatggt
     3361 aaaattacgg ttgaactaaa aacaccttta gagaataaaa atgatttgac attatgttat
     3421 tctccaggtg tggcagagcc tgttcgccaa attgctaacg atgaagaaag agtttgggat
     3481 ttaacaataa aagggaatac tgttgctatt atttcagatg gttcatcaat tttaggatta
     3541 ggaaatttag gtgctgaagc atcaattcca gtgatggaag ggaaagcaat gttgtttaaa
     3601 cgatttggtg gtttaaatgc ttttccaatt tgtttagcta cgcaagatac agaggaaatt
     3661 attagaacaa ttagaaatat tgctccagtt tttggtggta ttaatttaga agacatttca
     3721 tctccacgtt gttttgaaat tgaagataga ttacaaacat tgggcattcc tgtttttcac
     3781 gatgaccaac acggtacagc aattgttgct ttagctgctt tattcaatgc gtgtaaatta
     3841 tttaaaaaga actttggaga tttaaaggtg gttatcaatg gtgcgggagc tgctggatta
     3901 gctataacta aattaatttc ttgcgacgat ttagaaacag atatttgtac gcctgttcgt
     3961 gaaataatta tgtgtgatac caaaggtatt atccataaag acagaccaga tttgaacgat
     4021 tttaaaagag atattcttac ctataccaac aaaaatcatg taaaagggac tttgaaagat
     4081 gctattaaag atgctgatgt ttttatcgga gttagtcaag gaagtatttt gtcttctgaa
     4141 gatattacga caatgaataa agatgcaata attcttgcat tggcaaatcc tacacctgaa
     4201 atttctcctg aattagctta taaaggtggt gctggtgtgg ttggtactgg acgaagtgat
     4261 ttgcctaatc aagtgaataa tgttttagca tttccaggta tttttaaagg tgctttggct
     4321 gttcgagcta gacgtattac taatagaatg aaaattgcag ctgcttatgc cattgcagat
     4381 tgtgttgtaa atttagaacg tgattgtatt attccaaatg ccttagattt gggtgtggct
     4441 gatagagttg ccgaagccgt gatgaaagct tatttagaag ataaaaactt agaagcacct
     4501 aagcacgaat tataattcgt ttttttattt tttaattcta tttattctga tagaaagtaa
     4561 ttaattttga gtatcatacg ttttttgata ttggttaagc tatacatcaa atcatataga
     4621 atttaaacat acaaaatgaa aaataaaatt acaaccatag catttgacgc agatgatact
     4681 ttatgggtaa atgaacctta ttttcaagaa gccgaaaagc aattttgtgc cttacttgaa
     4741 aattatcttc cgcaacatac tatatcgcaa gaacttttta aaaccgaaat gaagaatttg
     4801 catctttacg gctatggaat taaaggcttt ttgttgtgta tgatagaaac cgcaaatagc
     4861 gtttcaaata gaactgtttc tttaaatgtc attgataaaa tcattgaaat tggacacgac
     4921 ttgctgcaaa aacctattga attactacaa ggtgtgcaag aaactttgga agccttgaat
     4981 ggagattata aattggtggt cgctaccaaa ggcgatttgt tggatcaaga aagaaaattg
     5041 aaaaactcag gattggaaca ctactttcat catatcgaaa ttatgagtga gaaaaaagag
     5101 agtgattatc aaaaactatt taaacatttg gattgccaat ctgaaaactt tttaatgttg
     5161 ggcaattcca tcaaatctga cattatttct attttagaat taggtggcaa tgctgctcat
     5221 attccatacc atgttacttg gacacacgag caacaagaag tcaatttgaa acacaataat
     5281 ttcattcaat tgaaatcaat taatgagatt ttaaattatc tgaaatagca atggaaatta
     5341 atattcgaca agaagcttta gtcgattatc cgatagtgtt tgatttaatt gaacaagcat
     5401 ttcgtgatga aattataagc gataaaaaag agcagttttt ggtggaacga ctaaggaaat
     5461 ctgatggatt tattccaaaa ttatcattag ttgctgaagc aaaccaaaaa atagttggct
     5521 acattctgct cacaaaaatc atcattgaaa cgaatacaga aagtgtaact tctttagcat
     5581 tagcaccagt tgctgtattg ccaacttttc aaaagaaagg aataggtgga agattgatag
     5641 aggaagccca tcacattgca aaaggtttag gctatacatc ggttatttta ttaggacacg
     5701 aaacctatta tcctaagttt ggatataaac ttttgaagga gtttaacatt acacttccat
     5761 ttgtagctcc taaggaaaat tgtatggcaa ttgaacttat ccctgatgct ttaaaaaata
     5821 aagaaggaaa agtgatttac ccaaaagaat tttttgaata gaaaaaagag ataaaatgaa
     5881 aaaattaata gacctcgaca attggaacag aaaggagcat ttccttttct tttcaaaatt
     5941 tgacgaacca ttttttggtg taaccgtaaa agtggattgt acaattgcct atcagaaagc
     6001 aaaagaaaaa ggtgtttctt ttttcttata ttacttatac agagctttga aagctgctaa
     6061 tgaagttgaa aatttcagat acagaataat agaaaatcaa ccttatgatt atgcaacaac
     6121 aaatgcttcg ccaacaatta atcgaccaaa tggaacattt ggatttgctt atattaatta
     6181 ttttgaaaat gaaaataaat tttatgaata tgcattgcaa gaaatagaaa atgtaaagaa
     6241 aacaagtagc ttaatgccgt ctatttctgg agaaaatgtc attcattttt cagctattcc
     6301 gtggattgac tttacaagta tttcacacgc aagaaatttt tcacatccag actcttgtcc
     6361 taaaatttca tttggtaaaa tgacagaaaa taatgggcaa aaagaaatgt ctgtttccat
     6421 tcacgtacat cacgcattaa tggacggcta tcatgtaggc ttatttattg aaaaatttca
     6481 aagtctatta agcgaagctt gatgaaagcc taaacgatac tatttagact aatatttcag
     6541 acttaaaaaa aacttacaat tttctattta aagaaatcta ctaagtattg tgtaacctct
     6601 tttggacagt cgttttgagc ccaatgcgaa caatttttga tgtattttat atcaagtgta
     6661 ttgtccacat aagcttgcgt attataagtc aattctttgc ttaatgcctt atcatcttca
     6721 ccccaaatta ataatgttgg tactgttact ttttgcttaa atacagacat tttaggtttc
     6781 gtttgcatca tagcacgata ataattaata gaacctgtta aagcaccagg ttctttgtaa
     6841 gctctcacaa aagcctgtaa atctttgtct gtaaaatgtt caggatgatg catccaacct
     6901 ctgaaaaata atttaaaaaa ttgtggaagc gtattgccta atataaattc aggaattagt
     6961 ggtaattggt gcataaacat ataccaactt tttaataatt gtgttggatt actttttatc
     7021 atcttcaaca tttcctttgg atgcggacag ttgataatcg ctaatttttc taacatattt
     7081 ggatagatag aagcaaaagt ccatgcaatt gcaccgcccc aatcatgacc aactacataa
     7141 gcttttttaa atcccatttg ttttatcaat tcagcaacat cagaagcaac aacatctgct
     7201 ttgtaatttt gtatgccttt aggtttgtcg cttttattaa acccacgcaa gtcaggagct
     7261 attacagtat attcttttgc taattcaggc atttgataac gccatgtgta ccaaaattct
     7321 ggccaaccat gtaataatac cactaatttt ttgccattgc cctgttgtgc tacatgcaaa
     7381 aaaatatcat ttactttaac ttgtttgtgt gtaatatcgt ataacatttg cttaaaattt
     7441 atttttacct aaaatttctc taaaacctat tctgattttt ttcctaaatg taaataattt
     7501 atttattcga acctgctttt tgagcttaaa agtattcggc agatagtcag tttttatctg
     7561 atgtttagca attgtagaat ctttatcatc aattacttct aaaattacgg aactatcttg
     7621 atttacagta acttttccat aagcatgatt gaagttttta ttctgaatgc cttgtccacc
     7681 ttgattccaa aggttaagtt tgaaattgtt gtaaataatg cttgcaatat gcgaattaac
     7741 ttgagctaaa ccaccagcca taatttctgg aaatccggcg tttttcccat cgtcaattgc
     7801 cgatgaatgt gcgtctccac ttagaattat tatattttta atttcattat ccttacaaaa
     7861 atttatcagt ttttgttgtg aatttggata ggcaaaccac atagccgata atgaggcagc
     7921 aatataagca ccatttttcc cattaggtaa tattctattt tgcaaacgca tacaaatatt
     7981 aaatactttc ttgtatgatt tattaaaagt tactcctgaa acaataaatt tccattttgc
     8041 ttcagatttt ttgagtccat ctaataacca tttcatttgt gtagaatcta atatttcgtg
     8101 ttcatttggg ttgctgtatt ttaattttcc ttttttatta gttgtaaaaa tttctgtatt
     8161 tggtgttctt gtagagcgat tatctaatac aaagaaatcg gcatttccat aagagaattt
     8221 atggtaacta tcttgattat ttacgtcatc ataacttgga aagaattcag ctaaactctt
     8281 ttttaaaatg gtttttaagg aatcaggata aggaatttct ttaacttgtg tgcttttgtt
     8341 attggcttca atcactgtaa aactagattt tgaaaaatca tcataaatgc catcttcatc
     8401 atcaaatata taatcaatag gtgtattttt taaaaagtta cctaaaagtt tataacgcgc
     8461 tataaattta tctctaagat gaaataattc tatttgttca gacaaatcag tagttgcatt
     8521 gtacttatta tacaaccaat ctccaagatg aataaagaag ttaggttttt gtttttctat
     8581 tgttttaaaa attccaaaat cttgcgattg ttctgtgcaa gagccaaaca caaaactaaa
     8641 tttctcagta ctattttttt taggtgcagt tttgaatgtg ccacgaaact cttcagtatt
     8701 gttttttgtt tttaaggtat aataacaagt ttggttgggc attaattctg aaattgtcag
     8761 tttattacaa aagaaaatgc tgtcttctgt atggtgttct atcgacataa taccttcttg
     8821 attgctaatt gttaaagtat aatcaatcaa agtatcgcta aaaaatagaa tctttgcact
     8881 attttcagta attgcaccta ttattggttg atgtaaaatg tgttgtgaaa aaatattatt
     8941 tacattaaat aggagtgtaa gaaaaatgag tgtatttttg tgcagttttt tcatattcaa
     9001 atttaattct tattttagta aaagaagatg ttttctgata agataatatt tatagtaggt
     9061 aattcgagga gcggaacgac aatgatgttg cggattttta ataaccacga ccaattgatg
     9121 gttttgaacg aattacactt ttttgaagaa ttgtggtcgc caatagataa aggaaaatca
     9181 atatcaagag cagatgctat ttttttaatg caaaaacttt tacttataca acggaaaggc
     9241 tataaagttg gttttgatga tttttcaatt ttttatgatg aagccaagca agttcttgat
     9301 aaatttgagg ataaggattt aatccctgaa aatatttttt atcaatttgc taaaagagaa
     9361 gttgcattaa acggaaaatc tatcgtttgt gaaaaaacac ctcaaaatgt tttttattta
     9421 aaagagattt ttgagttgtt tccaaatgcg aaagtcatta atatggttcg agatcctcgt
     9481 ggtgtaatgc tttcacaaaa aaataaatgg aatagaagga atttaggcgg ttggtatatt
     9541 actaaaaaag aggcaaggag attgcgaatt aattaccatc caattacatt gacaactttg
     9601 tggaatgctg caattaatgc ggtcaaaaaa aatgaagatg accaacgtat cttaactgta
     9661 atttttgaag aattagttaa tcagcctgaa caggaaatta aaaaaatatg caaacatatt
     9721 gat
//
LOCUS       DAOQCW010000098         9599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_2697_length_9599_cov_5.050189, whole genome shotgun sequence.
ACCESSION   DAOQCW010000098 DAOQCW010000000
VERSION     DAOQCW010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9599
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2697_length_9599_cov_5.050189"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..1028)
                     /locus_tag="PLS16_08910"
     CDS             complement(120..1028)
                     /locus_tag="PLS16_08910"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNJ61241.1"
                     /translation="MSWQKTIAKQTEITGIGLHTGKQCKVIMLPALPNEGLVLLKNNQ
                     KYPISLNNVVDTTRSTNIKIDDEVIYTVEHLLSAVVGLQIDNLILEIEGGEIPILDGS
                     AKLFVDKIREVGFKEEAIPKLNYIVQENIEWKDDETNAEYLLIPSSSFSIICIVDYHS
                     KSLGKQYASLNTWEDYATEIAPAKTFCFLHEIEMLYKSGLIQGGNLTNALVFSEFNIE
                     PEKAKWLTDTFNQPIFSVPEKGILNPIYQTFDNEAARHKTLDLIGDLSLLGIGLQGRL
                     IVIKPGHTSNIRFSKYLKEYIGAKIK"
     gene            1119..2408
                     /locus_tag="PLS16_08915"
     CDS             1119..2408
                     /locus_tag="PLS16_08915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNJ61242.1"
                     /translation="MDTYGLILLIAMPVFIVCILIELLIYQLKKIDYNYMDAIASLYS
                     GMTNTLKDVLGLTIVVISYDYLLQNLALFSWGKPNIWMYIITFIILDFGGYWYHRISH
                     EVNYFWNIHVVHHSSEYFNLPCALRQQFATITNILSILITGIALSILGIPKDVIVIVA
                     PLHLFLQFWYHTNLINKMGFLEYFLVTPSHHRVHHSMNKIYMDKNYSQIFIIWDKLFG
                     TFQAELDTEKCIYGIRHQPKTWNPFIINFQHLWSIVKDAFRTKNWKDKLLIWIKPTGW
                     RPKDVAQKYPIESITDDNVYNFIKYNPTYSNSFKFWSLVQLILSFLLMAFLFYNLPNL
                     SKSNHFILYGLFLLFSIFSITAIMDKKSYGIYTEILRLIFATYFIFTTQNWFGINQFY
                     KNAYIFVFLQFIISVFVSLFFYYKEFKPAYQIYKIPT"
     gene            2513..4501
                     /gene="rnr"
                     /locus_tag="PLS16_08920"
     CDS             2513..4501
                     /gene="rnr"
                     /locus_tag="PLS16_08920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HNJ61243.1"
                     /translation="MKKTPKRKHQTTKDSNIKKTKVAHKEPLLVGNELIGTIDIARTG
                     DAYVIIENIENDIFIHRKNVHGAFDGDTVKVKRIHNPRSNRKVGIITEIIERKRSIFI
                     GVADKQSKYCFVKADNVSIKNDFFIPEKYENNVKHGDKVVIEFIEWKKKDKNPIGKIS
                     EILGQAGSNDIEMKSILVENGFFTSFPTEVLEEAEAIPTKISNSEIKKRKDFRNVTTL
                     TIDPDDAKDFDDAISFQKVTDDTFQIGIHIADVSHYVQENTALDKEAYKRATSVYLVD
                     RVAPMLPENISNIICSLRPNEEKLCFSAVFIIDKNAKVLDIWFGRTVIFSDRRLTYNQ
                     AQEIIEGKDDILNFEINTLNKIATQLRKIRFKNGSLDFDAPEPKFQLDETGKPIGIII
                     KERKAAHMLIEEFMLLANRYVAYYVGKEKNASGKTPMVYRVHDLPNIEKLQDFALFAA
                     KFGYKANFDNPKKITQTLNTLMEKIHGKPEQNLLESLAIRSMAKAIYTTKNIGHYGLG
                     FEYYTHFTSPIRRYPDIMVHRLLQIVLDGKKMPALNELEQKCGHSSEREKAAAEAERT
                     SVKYKMAEFMLDRIGESFQGVISGVKNWGIYVELPDYNCEGLLRTEYLTDDTYLYDEK
                     NIQFVGKRTGNIYQLGETIEVILENVDIERKTIDFRLK"
     gene            4642..4718
                     /locus_tag="PLS16_08925"
     tRNA            4642..4718
                     /locus_tag="PLS16_08925"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4676..4678,aa:His,seq:gtg)
     gene            4861..5214
                     /locus_tag="PLS16_08930"
     CDS             4861..5214
                     /locus_tag="PLS16_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNJ61244.1"
                     /translation="MIEYLPILFMFVVAAGFVVFTMVVTHLLGPKRKSKIKLETFECG
                     IESQGNARLPFSIKYFLVAILFVLFDVEVIFMYPWAVNFKQLGLAGLLEMFAFIGFFL
                     IGFFYIIKKGALKWE"
     gene            5252..5806
                     /locus_tag="PLS16_08935"
     CDS             5252..5806
                     /locus_tag="PLS16_08935"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HNJ61245.1"
                     /translation="MSTIPVDISPAYEPAGYGGEGFFATSLDKVVGLARKNSLWPLPF
                     ATSCCGIEFMSTMSATYDFARFGAERLSFSPRQADLLMVMGTISKKLAPVLRQVYEQM
                     AEPKWVLCMGACASSGGIFDTYSVLQGIDRIIPVDVYVPGCAPRPEQVLDGIMQIQKL
                     AESESIRRRDSTEYKQMLSRYGIE"
     gene            5818..6315
                     /locus_tag="PLS16_08940"
     CDS             5818..6315
                     /locus_tag="PLS16_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNJ61246.1"
                     /translation="MTELILQKLKEKFPEAVVGFEQQYDFPIFIIDKKHIKEVLSELK
                     ADEELNFHFLTTMCGIHYPDNPQESEFCMMYQLHNMPKNIRIRLKTFMPKSDLHVPTI
                     TDLWKTANWMERQEYDFFGFIFDGHPNLTRILNMDEMNYHPLRKEYPLEDLRRDDKDD
                     KMFGR"
     gene            6325..7515
                     /locus_tag="PLS16_08945"
     CDS             6325..7515
                     /locus_tag="PLS16_08945"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNJ61247.1"
                     /translation="MKQVEDIEEKHYTTLNLGPTHPATHGIFQNVLTMDGETIVDSVQ
                     TVGYIHRAFEKLGERRPYNQINVLTDRLNYCSAPINNMGWFMTVEKLIGAEIPKRAQY
                     LRVIIMELSRIADHIICDTVIGVDSGALSGFLHLYQSREKIYDIFESICGARLTTNIG
                     RIGGLERDFSESTWGHLNQFLKEFPVKLKEFQKLMDRNRIFMDRIIGVGRISAEDALN
                     YGFTGPNLRAAGVDYDVRVMNPYSSYEDFEFDIPVGTSGDTFDRYSVRIEEMWQSLRI
                     IEQAIAKLPDGAFHADLPYFYLPPKADVYSKMEALIYHFKVIMGETELPVGEVYHAVE
                     GGNGELGYYIISDGGRTPFRFHIRRPGFIYYQAYPHMIKGSMLSDAIITMSSMNVIAG
                     ELDA"
     gene            complement(7955..8401)
                     /gene="rplI"
                     /locus_tag="PLS16_08950"
     CDS             complement(7955..8401)
                     /gene="rplI"
                     /locus_tag="PLS16_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNJ61248.1"
                     /translation="MEIILLENVKNLGLKYDVVTVKPGYGRNFLIPKGLAKIANASNK
                     KHLEEIVKQQSAKIAKLTEEMKQLAEKLSQSKIFVGSKAGTTGKLFGSVTNIQLADAL
                     KKQFDLDVDRKKIHIQGEVKEVGTYKAVVGLYKEITAEVQFDVVAE"
     gene            complement(8411..8677)
                     /gene="rpsR"
                     /locus_tag="PLS16_08955"
     CDS             complement(8411..8677)
                     /gene="rpsR"
                     /locus_tag="PLS16_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNJ61249.1"
                     /translation="MATQDEIKFLNAPKLGEQKKKYCRFKKMGIKYIDYKDPDFLLRF
                     VNEQGKLLPRRITGNSLKYQRKVSQAVKKARHLALLPYVTDLLK"
     gene            complement(8680..9033)
                     /gene="rpsF"
                     /locus_tag="PLS16_08960"
     CDS             complement(8680..9033)
                     /gene="rpsF"
                     /locus_tag="PLS16_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNJ61250.1"
                     /translation="MNNYETVFITTPVLSKDEYTRTVKKYTDFIEKNGGEVISKEDWG
                     LKQLAYPIQKKTTGYYTLLDFKLDPLLVKKFEIEFRRDENVMRFLTTKLDKHALAYSV
                     QRREKLNGKTQKTEA"
     gene            9194..>9599
                     /locus_tag="PLS16_08965"
     CDS             9194..>9599
                     /locus_tag="PLS16_08965"
                     /inference="COORDINATES: protein motif:HMM:NF017551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HNJ61251.1"
                     /translation="MCLIAFAINVHPDYPLILIANRDEFYNRPTRSAQFWEAEGFADI
                     LAGKDLKAGGTWIGINKNKRWAALTNYRNLHNLKENPPSRGGLVLDFINTNSSSENYL
                     SNIQKDAALYNDFNLLLFDGKEITYYSNISNTI"
BASE COUNT         3320 a         1450 c         1475 g         3354 t
ORIGIN      
        1 ttagagaatt taaaactcat ccaaacagga caattactga ctgcatttat ttatattagt
       61 gcaagtattt tgttaggaat tgtagcaact tggctgggta tatttactac taaataagcc
      121 tatttaattt ttgctccaat gtattctttt agatatttag aaaatctaat attggatgta
      181 tgtcctggtt ttatgacaat taatctacct tgtaaaccaa ttcccaataa cgacaaatca
      241 cctattaaat ctaaagtttt atgtcttgcg gcttcgttgt caaaagtctg atatatagga
      301 tttagaatac ctttttcagg aactgaaaaa attggctgat taaaggtatc tgtgagccat
      361 tttgcttttt caggttcaat attaaattct gaaaaaacta aagcattagt caaatttccg
      421 ccttgaatta atccgctctt atataacatt tcaatttcgt gtaaaaaaca aaatgttttt
      481 gctggggcta tttctgtagc ataatcttcc caagtattca aacttgcata ttgttttccc
      541 aaactcttgg aatgataatc aactatacaa ataattgaaa aactagatga tggaattaat
      601 aaatattctg catttgtctc atcatctttc cattcaatgt tttcttgaac aatataattt
      661 aacttaggaa tagcttcctc tttgaagcca acttctctaa ttttatcgac aaaaagtttc
      721 gcacttccat ctaaaatagg tatctctccg ccttcaattt ccaaaatcaa attatctatt
      781 tgtaagccaa caacagcaga caataaatgt tctactgtat agataacctc atcatcaatc
      841 ttaatatttg ttgaacgagt agtatctacg acattattta aagaaatagg atatttttga
      901 ttgtttttaa gtaataccaa accttcatta ggcaaagctg gaagcattat taccttacac
      961 tgttttccag tatgtaatcc gataccagtt atttctgttt gctttgcaat tgttttttgc
     1021 caactcatat tctagaacac aataataaga aaatcaattt aattataaaa attttaacaa
     1081 aattattgta acttagatat cccaaaatat atttcactat ggatacctac ggtttaattt
     1141 tattaattgc gatgcctgtt tttatagttt gtatattaat agaattattg atatatcaac
     1201 taaaaaaaat agactataat tatatggatg ctattgcaag cttatattcg ggtatgacta
     1261 atacattgaa agatgttttg ggtctgacta tagttgtgat ttcgtatgat tatcttttgc
     1321 aaaatttagc actttttagt tggggcaaac caaacatctg gatgtatatt attactttta
     1381 ttattctgga ttttggtggg tattggtatc atcgtatttc tcatgaagta aattattttt
     1441 ggaatattca tgtagttcat cacagtagcg aatactttaa tttaccttgt gctttacgcc
     1501 aacaatttgc aacaataact aatattttga gtattttaat tacaggtatt gcattatcaa
     1561 tattaggaat tccaaaagat gttatagtaa ttgttgctcc cttacattta tttttacaat
     1621 tttggtacca tactaaccta attaacaaaa tgggtttctt ggaatatttt cttgtaacac
     1681 catctcatca tagagtacat cattctatga ataaaatata tatggacaag aattacagcc
     1741 agatttttat tatttgggat aaattatttg gtacatttca agctgaactt gacactgaaa
     1801 aatgtattta tggcattcga catcaaccaa aaacttggaa tccatttatt attaactttc
     1861 agcacttatg gtctattgta aaagatgctt ttcgaacaaa gaactggaaa gataaactat
     1921 taatttggat aaaaccaaca ggctggcgac ccaaagatgt tgctcaaaaa tacccaatcg
     1981 aatcaattac cgatgataat gtttataatt tcatcaaata caatccaacg tattcaaata
     2041 gttttaaatt ttggagtttg gttcaactta ttctatcgtt tttattgatg gcatttctat
     2101 tttataatct acctaatctt tcaaaatcaa atcattttat cttatacggt ttgtttttat
     2161 tgttttccat tttttctata actgcaatta tggataaaaa aagctatgga atttatacgg
     2221 aaatacttcg attaattttt gctacttact tcatcttcac aacacaaaat tggtttggaa
     2281 tcaatcagtt ctataaaaat gcatatattt ttgtattttt acaatttata atatctgtat
     2341 ttgtatcatt atttttctat tataaagaat ttaaacctgc ctaccaaatc tataaaatac
     2401 caacttaaat ttagtctatt tgcatcatct taaaaaacaa aaaataactt aggcattaat
     2461 tttgtatgta acaaaactta atgaaccaat acaatattca aatccaaata aaatgaagaa
     2521 aacgccaaaa agaaaacacc aaactaccaa agattctaac ataaaaaaaa caaaagtcgc
     2581 acataaagag cctctcttag ttggtaacga acttataggg acaattgata ttgcccgaac
     2641 tggcgatgct tatgttatta ttgaaaatat agaaaatgat atttttatac atagaaaaaa
     2701 tgtacacggt gcttttgatg gcgatactgt aaaagtaaaa cgtattcaca atccaaggtc
     2761 aaatcggaaa gtaggtatta ttactgaaat tattgagcgt aaacgcagta tttttattgg
     2821 tgttgctgat aagcaaagta aatattgttt tgtaaaagca gataatgtat ctatcaaaaa
     2881 tgattttttt attccagaaa aatatgaaaa caacgtaaag catggcgaca aagtggtgat
     2941 tgaatttatt gaatggaaaa agaaagacaa aaaccctatt ggaaaaatca gtgaaatatt
     3001 aggacaagct ggaagcaatg atatcgaaat gaaatctatt ttagttgaaa atggattttt
     3061 tacttcattc ccaaccgagg tattagaaga agcagaggct attcctacaa aaatttcaaa
     3121 ctcagaaatc aaaaaacgaa aagactttag aaatgttact acactaacaa ttgaccctga
     3181 tgatgctaaa gactttgacg atgctatttc attccaaaaa gttacagatg atacatttca
     3241 aataggcatt cacattgccg atgtgagtca ttatgtacaa gaaaatacgg ctttagataa
     3301 agaagcctat aaacgtgcaa cttctgtata tttagtagat agagtcgcac ctatgttgcc
     3361 agaaaatatt tccaatatta tttgttcgct cagaccaaat gaagaaaaac tctgtttttc
     3421 agcagtattt attattgata aaaatgctaa agtattagat atttggtttg gtagaacagt
     3481 aatattttca gatagaagat tgacttataa tcaagctcaa gaaattattg agggcaaaga
     3541 cgatatactt aattttgaaa taaacacact caataaaata gcaactcaac ttagaaaaat
     3601 acgttttaaa aatggctctt tagattttga tgcacctgaa ccaaaatttc aattagatga
     3661 aactggcaaa ccaatcggca tcattatcaa agaacgaaaa gctgcacaca tgctgattga
     3721 agaatttatg cttttagcca accgttatgt tgcctactat gtgggcaaag agaaaaatgc
     3781 tagtggcaaa acacctatgg tatatagagt acatgattta cccaatatag aaaaactaca
     3841 agattttgca ctttttgctg ctaaatttgg atacaaagcc aactttgata atcctaaaaa
     3901 aattacacaa acactcaata cgctaatgga aaaaattcat ggcaaacctg aacaaaattt
     3961 attagagtct ttagccatac gaagtatggc caaagcaatt tataccacta aaaatattgg
     4021 acattatggt ttaggatttg aatattatac gcattttacc tctcccattc gtcgttatcc
     4081 agatattatg gtgcatagat tattgcaaat agtattagat ggtaaaaaaa tgccagcact
     4141 taatgaattg gaacaaaaat gtggccacag ttctgaaaga gaaaaagcag ctgctgaagc
     4201 tgaacgaaca tcagtaaaat acaaaatggc agaatttatg ttagatagaa ttggtgagtc
     4261 attccaaggc gtaatttctg gagtaaaaaa ctggggaatc tatgtcgaat tacctgatta
     4321 taattgtgaa ggtttattaa gaactgaata ccttacagat gatacctact tatacgatga
     4381 aaaaaatata caatttgttg gaaaacgaac aggaaacatt tatcaattag gagaaactat
     4441 tgaagtaatt ttagaaaatg tagatatcga aagaaaaact atagatttca gattaaaata
     4501 aaatttcaat tgttgaagta taaatcgtat ataagttcca tttacaactt aaaaaaatct
     4561 ataattttat tagacaacaa atatttaaac taaaaactac tacactatta aaaaaatata
     4621 ctatatttgc accctcaaac ggacatcgta gctcagttgg tcagagcatc ggattgtggt
     4681 tccgagggtc gtgggttcga gccccatcga tgtccccact ttttattaat atttccactt
     4741 caattttctt tttccattca atattcttag aatataaaat tattcaaaag ccaacatttg
     4801 tttaaagtct ttttgctact tttgaagtta taatgcgtat atttgcataa aatttaggaa
     4861 atgattgaat atttgcctat tctgtttatg tttgtagttg ctgcaggttt tgtagttttt
     4921 acaatggtgg ttacccactt attaggtccg aaaagaaaat caaaaataaa attagaaact
     4981 tttgaatgtg gtattgagtc gcaaggcaat gctcgattac cattttcaat aaaatatttt
     5041 ttagtcgcca tcttatttgt attgtttgat gtggaagtca tttttatgta tccttgggct
     5101 gttaatttta aacaattagg acttgctgga ttattggaaa tgtttgcttt catcggattc
     5161 tttttaatag gctttttcta tattataaaa aaaggtgcat taaaatggga ataaaacaaa
     5221 acttttaaga taataatttt gtattagata tatgagtaca attcctgtag atattagtcc
     5281 ggcatacgaa cctgctggtt atggaggaga aggttttttt gctacttctt tagataaagt
     5341 agttggtttg gcacgtaaaa actcactttg gccattacct tttgccacat cttgttgtgg
     5401 tatagaattt atgtccacaa tgagtgccac ttatgacttt gcaagatttg gagcagaacg
     5461 tttaagcttc tctccaagac aagcagattt attaatggtt atgggtacaa tttcaaaaaa
     5521 attagcaccc gtattacgtc aagtgtacga acaaatggct gaaccaaaat gggtactttg
     5581 catgggtgct tgtgctagca gtggtggcat ttttgatact tatagcgtat tgcaaggaat
     5641 tgatagaatt atccctgttg atgtatatgt accaggttgt gcacctcgtc ctgaacaagt
     5701 tttagatggc attatgcaaa tacaaaaact cgctgaatct gaatctataa gaagacgaga
     5761 ttctacagag tataaacaaa tgttatcaag atacggaatt gaataaaatt agtgtgcatg
     5821 actgaattaa tcttacaaaa attaaaagag aaatttcctg aagctgttgt tggttttgaa
     5881 caacaatacg atttcccaat ttttataatt gataaaaaac acattaaaga agtactttcg
     5941 gagcttaaag cagatgaaga attaaacttc cacttcctta caacaatgtg tggcatacat
     6001 tatccagaca atccacaaga atcagaattt tgtatgatgt atcagttgca caatatgcct
     6061 aaaaatatcc gtatccgatt gaaaactttt atgcctaaat cagatttgca tgtacctaca
     6121 attacggatt tatggaaaac agccaattgg atggaacgtc aagaatatga tttctttggg
     6181 tttatttttg atggacatcc aaatttgaca cgcatactta atatggacga aatgaactat
     6241 catcctttga gaaaagaata tcctttggaa gatttgcgtc gtgatgataa agatgataaa
     6301 atgtttggca gataatttat tacaatgaaa caagtagaag acatcgaaga aaagcactat
     6361 accacgttaa atttaggacc aacacatcct gcgacacacg gcatatttca aaatgtgctt
     6421 actatggatg gtgaaactat tgtggattcc gtacaaactg ttgggtatat tcatcgtgct
     6481 tttgaaaaat taggtgaacg tcgtccatat aatcaaatta atgtcttaac agaccgattg
     6541 aactattgct ccgctccaat taataatatg ggctggttta tgacagtaga aaaattaatt
     6601 ggtgcagaaa ttccaaaacg tgctcaatat ttaagggtaa ttattatgga attatcacga
     6661 attgctgatc atattatttg tgataccgtt attggtgttg atagtggagc attatctggc
     6721 tttttacatt tatatcaatc aagagaaaaa atttacgata tatttgaatc tatttgtgga
     6781 gctagattaa ctacaaatat tggaagaatt ggtggcttag aaagagattt ttctgaatcg
     6841 acttggggtc atttgaatca atttttaaaa gagtttccag ttaagctaaa agagtttcaa
     6901 aaattgatgg atagaaaccg cattttcatg gatagaatta tcggcgttgg tagaatttca
     6961 gcagaagatg cattaaatta tggatttaca ggtcctaatt tacgagcagc aggcgttgat
     7021 tatgatgtta gagttatgaa tccatattct tcttatgaag actttgaatt tgacattcct
     7081 gttggaacat ctggagatac atttgataga tattcagttc gtatagaaga aatgtggcaa
     7141 agtttaagaa ttattgaaca agccatagca aaattacctg atggtgcttt ccatgcagat
     7201 ttgccttatt tttatttacc tccaaaagct gatgtttata gtaagatgga agctttgatt
     7261 tatcatttta aagtcattat gggagaaaca gaactgccag ttggtgaagt ttatcatgct
     7321 gttgaaggtg gaaatggcga attaggttac tatatcattt cagatggagg aagaacgcct
     7381 tttcgttttc atatccgaag accaggtttt atttattatc aagcctatcc acacatgatt
     7441 aaaggcagta tgttaagtga tgccattatt acaatgagta gcatgaatgt aattgctgga
     7501 gaattagatg cttaatttaa gtttactttt cctaaaattt atttactaag attgcaaata
     7561 gtgatttcca aaaacaacta atgagaaaaa ttcataatct cacttctcaa attgtctgaa
     7621 ctatgatttt tatgattaaa tgatagacag gatttttata atgtagtaat gaaataatgt
     7681 agtaatgaag taattaaatt ctcactactt aatactaaat actttctact catctaacta
     7741 ccaaatactt tctacttatt actcaaaaaa aagtcttggc tcttggctct aataatcttg
     7801 actctaatct ctcactactc aatactttct acttaatact caaaatagtc ttgattcttt
     7861 aaaagctaaa cctaataagc aaatatattt gatgtaatga cataaaaaaa accacagcct
     7921 aaactgtggt tctattttaa caagaataat aaaattattc tgctactaca tcaaattgta
     7981 cttcagcagt aatttcttta tataaaccaa caacagcttt ataagttcct acttctttga
     8041 cttctccttg aatatggatt ttctttctat ccacatctaa atcaaattgc ttttttaaag
     8101 catctgccaa ttggatattg gtaacactac caaataattt tccagtagta cctgcttttg
     8161 aaccaacaaa aattttagat tgactcaatt tttctgctaa ttgcttcatt tcttctgtta
     8221 atttagcaat ttttgcagat tgttgtttta caatttcttc taagtgtttt ttgttagaag
     8281 cgttagcaat ttttgctaat ccttttggga ttaaaaaatt acgaccatag ccaggtttta
     8341 ctgtaactac atcatatttt agtcccaagt tctttacatt ttctaataat ataatttcca
     8401 tggtgtattt ctattttaat aagtcagtaa catacggtaa taacgctaag tgtcttgctt
     8461 ttttaacagc ttgagaaact tttctttgat atttcagaga gttacccgtg attcttcttg
     8521 gtaataattt tccttgctcg ttcacaaatc ttaataagaa atctgggtct ttgtaatcaa
     8581 tatatttgat tcccattttc ttaaaacggc agtatttttt cttttgctca cctaattttg
     8641 gagcgtttaa gaatttaatt tcgtcttgcg ttgccataat taagcctccg ttttttgtgt
     8701 tttaccattt aatttctctc ttctttgtac tgaataagcc aaagcatgtt tgtctaattt
     8761 agtcgttaaa aaacgcatca cgttttcatc tcttctaaat tcaatttcaa attttttcac
     8821 taaaagtgga tctaatttaa aatctaacaa ggtataataa cctgttgttt tcttttgaat
     8881 aggataagcc aattgcttta aaccccaatc ttctttgctt attacttcgc cgccgttttt
     8941 ttcaatgaag tcggtgtact tctttacggt tcttgtgtac tcatctttag atagtacggg
     9001 cgttgtaata aaaaccgttt cgtaattgtt cataaataaa ttaattttga gatgcaaagg
     9061 tagttctttt ttttgtaaaa gtgaaacatt atatttatta ccttaaatta agaaaacatt
     9121 ataaaccaac gattattcaa gaaaaactcc ttaaatcaaa caaaatagta tctttaattc
     9181 ataaattttc aaaatgtgcc tcattgcgtt tgctattaat gttcatcctg attatccgtt
     9241 gatactaatt gccaacagag atgaatttta taatcgacca actcgttctg cacaattttg
     9301 ggaagctgaa ggttttgctg atattcttgc tggcaaggat ttaaaagctg gtggaacttg
     9361 gattggcata aacaaaaata aacgttgggc agcactcact aattatcgga atttacacaa
     9421 cttaaaagaa aacccaccat ctcgtggtgg tttagtattg gattttataa atactaacag
     9481 ttcttctgaa aattatttaa gtaatattca aaaagatgca gccttgtaca atgattttaa
     9541 tttattgctt tttgatggca aagaaataac ctattattct aatatttcca ataccattc
//
LOCUS       DAOQCW010000099         9597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_2699_length_9597_cov_5.650597, whole genome shotgun sequence.
ACCESSION   DAOQCW010000099 DAOQCW010000000
VERSION     DAOQCW010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9597
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2699_length_9597_cov_5.650597"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            731..1936
                     /locus_tag="PLS16_08970"
     CDS             731..1936
                     /locus_tag="PLS16_08970"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNJ61252.1"
                     /translation="MLVIVAALGYFVDVFDLLLFGMVRIGSLRDLGVPEADLESVGMH
                     LDNLQMIGMLLGGLIWGIIGDKKGRLSVLFGSILTYSIANFLNAHINSVEQYAVLRLI
                     AGFGLAGELGAGITLINETLSKEKRGLAAAFVAGFGVLGAVFGALLVLEIGDWRMCYM
                     IGGGMGVALLILRISVFESGMYQNVKHKTSQLGDLRLLFGNKKNLKKYLAIIFIAVPI
                     WYVVQLYSKYAPELAEALGLHVENKKEVAVYAIMAIYTGLTFGDVACGFYSNWVKSRK
                     KAIITYLSFLVGLIVIFWFVAKISFAVFYGMIFLMGLGIGYWAVFVTTASEAFGTNIR
                     STVSNTVPNFVRGFVVIINFMYISFKDMTSSTITANILTGIICVGLALYAWTQLEETY
                     GKDLDYVEQ"
     gene            2054..2251
                     /gene="rpmI"
                     /locus_tag="PLS16_08975"
     CDS             2054..2251
                     /gene="rpmI"
                     /locus_tag="PLS16_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNJ61253.1"
                     /translation="MPKQKTNSSAKKRFKVTGSGKIKRAKAYKRHILTKKTTKQKKRL
                     GKAGLVHTSDMNNAKKLLALI"
     gene            2332..2676
                     /gene="rplT"
                     /locus_tag="PLS16_08980"
     CDS             2332..2676
                     /gene="rplT"
                     /locus_tag="PLS16_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNJ61254.1"
                     /translation="MPRSVNSVAAKARKKKVYKLAKGYFGRRKNVWTVTKNAVEKGLA
                     YAYVGRKLKKRDFRSLWIVRINAAVRNYGMSYSVFINKLNKAGVELNRKALADLALNN
                     PEAFEAVVNKVK"
     gene            2869..4323
                     /locus_tag="PLS16_08985"
     CDS             2869..4323
                     /locus_tag="PLS16_08985"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNJ61255.1"
                     /translation="MEKNTYDVIIMGAGLSGIGTAYHLQKNCKGKSYVILENRPSLGG
                     TWDLFRYPGIRSDSDMFTLGYSFKPWNHSEPIADGGLILEYLKEAASENGIDKNIKFN
                     TKINEAKWDSSTNTWTVTCHDNKTKKSVSYTAKYVISCLGYYNYDKGHTPKFNGVKNF
                     KGEILHPQFWPENYDYSGKEVVIIGSGATAVTLVPAMTDKAKHVTMLQRSPTYIASIP
                     NRSLIPQAVLKNLPERVKYDLNRAAQIGLQVSSYNLCQNQPELMKMYFRQHVKKQLPK
                     GYDIDKHFTPKYNPWDERLCAVPNGDLFKAIRSGKAEVVTDHIDKFVEDGILLESGKK
                     LKADIIITATGLELQMLGGIKTFIDDKEFKASDGIIYKGAMIKDVPNFAFVLGYTNSS
                     WTLKSDMIGEYFCKLINETEKKNKKVFVVKSDKPIEKEPALNFGAGYVQRAIQAGALP
                     SQGKEYPWKLKQNYLVDAANFKVRNIQDEFLKFV"
     gene            complement(4372..5178)
                     /locus_tag="PLS16_08990"
     CDS             complement(4372..5178)
                     /locus_tag="PLS16_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61256.1"
                     /translation="MLEKRWLYNQKMYAYSILILFLFLYGYRLYSGRMISDLYGQPLQ
                     APGLAYTYWLLLLAQIPQYIHQHFYIGLFIDVSILIIAIVLFFKYDNKYLAILFLTLI
                     FLQNSTVEIFANMHNKMPIAIFIAFFPLCFNKKYFEITLEFSRYFLAFILLSAAYHKF
                     HNGAAYTFMHYYNVLINQHIDLSLINPNHVCYKISQFLVQSPYYAFIAFSLLIIAQGV
                     FIIAFFTKKLDTILLVFFLLFALLGYLIMRIWTIDLLILAPTLLYQYRQK"
     gene            complement(5180..5716)
                     /locus_tag="PLS16_08995"
     CDS             complement(5180..5716)
                     /locus_tag="PLS16_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61257.1"
                     /translation="MKNWYFIRLYKANKYIFLVVLLFCFGQAFFSQKRIINFPWYTYD
                     MYSRPYNLDSAKDVVFLYINDTLYNHENLAIWKESVLFNTAKMYNNLSKNQFIDPAIP
                     TTNQVFRSMPECFKHYAVKHINNQVSNPEEYKNWLVQYIKDNTHTIPKKIDFRRVHLR
                     LEGHQYVYQYNDPFLIIQ"
     gene            complement(5722..6216)
                     /locus_tag="PLS16_09000"
     CDS             complement(5722..6216)
                     /locus_tag="PLS16_09000"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HNJ61258.1"
                     /translation="MEVALIITIGIILLVIGLASCVLPPLPGPPIAFIALLLARFGLN
                     KVQELPNWLLILYAAIIILVLIIDNFIPIWGTKKFGGTKAGIRGSFIGILVGIFLAPF
                     GGISIIICPFLGAIIGELIAGENFTLAVKSGIGSFVGFLLTSGIKIILVVTIGFHFIK
                     VTLF"
     gene            complement(6217..6825)
                     /locus_tag="PLS16_09005"
     CDS             complement(6217..6825)
                     /locus_tag="PLS16_09005"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HNJ61259.1"
                     /translation="MKNKLAAPIIIVLLVFCAWFFYKWKTSVPVSEVNSTIMLNKIEK
                     VMKMVNIEANFSELLTYEDYDYIDFPGFRKKAIIKVDAKVLAGFDMNKMKFEVDEINK
                     TIIIKNIPKSEVLAIDHQLSYYDMQQGIFNAFDEKTLTSLQEKAKALIAKKVQETDIL
                     QQADAQKLEIFSLIYYMAKSTGWKIEIEGKELKETNGIINYN"
     gene            complement(6981..8195)
                     /gene="rocD"
                     /locus_tag="PLS16_09010"
     CDS             complement(6981..8195)
                     /gene="rocD"
                     /locus_tag="PLS16_09010"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HNJ61260.1"
                     /translation="MSKMPAQYYIQLEERFGAHNYHPLPVVLSKGKDIFVWDTDEKKY
                     YDFLSAYSAVNQGHCHPKIIAALTEQAQTLTLTSRAFYNDALGIAEEFITHIFGYDKV
                     LMMNTGVEGGETAIKLARKWAYEVKGVEPNKAKIIFAENNFMGRTIAAVSASTDPSSY
                     NNFGPFVPEFYKIPYNDIPALEIALQDKNVAAFFFEPIQGEAGVMVPDDGYLQQVRAI
                     CSKNNVLMIADEVQTGIARTGKMLACDYETVKPDILILGKALSGGVLPVSAVLANNEV
                     MLTIKPGEHGSTFGGNPLACKVSIAALQVVIDENLAENAMKMGNIFRTEFANFEHHLI
                     KEVRGKGLLNAIEINQHNKEDLAWEICMTMKENGLLAKPTHGNIIRFAPPLTIQENDL
                     EQAINIIKKSLG"
     gene            complement(8208..9086)
                     /locus_tag="PLS16_09015"
     CDS             complement(8208..9086)
                     /locus_tag="PLS16_09015"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ61261.1"
                     /translation="MKTFIIGASGLVGGNCLEYFNAKNWENIGTYFSFKTDDTVFFDT
                     LNLDNPDNFDVKKFQPNVIVHCGALTHVDYCETNEQESYKKTVLSTKNIVDLAVQLNS
                     KLVFISTDYIFDGTNGPYAETDKPNPISIYGKHKLEAENYVLDNIKEAIVLRITNVYG
                     DEIRGKNFVSRIVEQAQNNQHLTLKLPIDQYATPINAADIARCLYLLLNDKQNGIFNI
                     ASTDYMNRVQLALRILHYFPNASYDLIPLKTTALNQPASRPLQGGLKNEKFATLYPDF
                     VFSTVDEYVSSKIDKQ"
     gene            9175..>9597
                     /locus_tag="PLS16_09020"
     CDS             9175..>9597
                     /locus_tag="PLS16_09020"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HNJ61262.1"
                     /translation="MKILGISAYYHDSAICLIEDGKILYAAQEERFSRIKNDENFPIL
                     AIQNCLSELQISLNDIRTIVYYEKPFLKIERILESYLDVAPHGFQSFLQAMHVWTKEK
                     LFIKQNIYKELKKIDQNFNKKNILMLFSEHHLSHLASAF"
BASE COUNT         3421 a         1357 c         1504 g         3315 t
ORIGIN      
        1 aaatctcact actcactact ttctactcta aaaattgtct gaactatgat ttttatgatt
       61 aaatgatgga caggattttt ataatgtagt aatgaaataa ttaaataatg taataatgaa
      121 tttctcaata ctcactactt tctacttaat actcgaaaaa agtcttggct ctcatctcta
      181 actacttaat actttctact taatactcat tttttaacca caaagtacac taagttttta
      241 cacggagttc acaaagatta atgtagtaat gtaataatga atttctcaat acttaatact
      301 caatactttt tactcaaatc tcaatactta atactcaata ctttctactc aaatctcact
      361 actcactact ttctactcta aaaattgtct gaactatgat ttttaggatt aaatgattag
      421 caggattttt ataatgaaat aatgaaataa tgaaataatg aaataatgaa ataatgtagt
      481 aatttctcac tacttaatac tcaatacttt ctactcaaat ctcactactt aatactttct
      541 acttaatact acaacaaagt cttgattctg agatttattg ctatttcacc aaaaaaacta
      601 agaaagtata atttctattt tcttatcttt agcatcaatt attcttcata aattatatta
      661 atgaaagtgg agaagaatat aaaattactt gaatatgtcc atgcctaatt ctaataataa
      721 aaatgctact atgttggtaa tagtagctgc actcggttat tttgttgatg tttttgattt
      781 attgttgttt ggaatggtgc ggataggaag tttaagagat ttaggcgttc ccgaagcaga
      841 tttagaaagt gtgggtatgc atttggataa tttgcaaatg ataggaatgt tgttgggtgg
      901 cttgatttgg ggaattatcg gagataaaaa agggcgactt tcggttttat ttggttctat
      961 tttaacgtat tccattgcaa attttttaaa tgcacatatc aatagtgttg aacagtatgc
     1021 agttttgaga ttaatagctg gttttggctt ggctggagaa ttgggtgctg gaattacctt
     1081 aatcaatgaa acattatcaa aagaaaaaag aggcttagca gctgcttttg ttgctggatt
     1141 tggcgttttg ggtgctgtat ttggtgcttt acttgtttta gaaattggag attggcgtat
     1201 gtgttatatg attggtggtg gaatgggtgt tgctttattg attctaagaa tcagtgtttt
     1261 tgaatctgga atgtatcaga atgtaaagca taaaacatca caattgggcg atttgagatt
     1321 attgtttggg aataaaaaga atttaaaaaa atacttagct attattttta tcgctgttcc
     1381 gatttggtat gttgttcaat tgtattcaaa atatgcaccc gaattggcag aagcattagg
     1441 tttgcatgtc gaaaataaaa aggaagttgc tgtatatgca attatggcaa tttatactgg
     1501 attaactttt ggcgatgtcg cttgtggttt ttattctaat tgggtaaaaa gtagaaaaaa
     1561 agcaatcatt acttatttgt ctttcttggt aggtttgata gttatatttt ggtttgtggc
     1621 aaaaattagc tttgcagttt tctatggaat gatattttta atgggtttag gaattggcta
     1681 ttgggcagta tttgttacga cagcatcaga ggcatttgga accaatatcc gttcgacagt
     1741 aagtaataca gtgccaaact ttgtaagagg ttttgttgta attattaatt tcatgtacat
     1801 ttctttcaaa gacatgactt catcgacgat tactgcgaat atacttactg gaattatttg
     1861 cgttggattg gcattgtacg cttggacaca attagaggaa acttatggaa aagatttaga
     1921 ttatgtggaa cagtaaaatt ttacatagaa tttaaacttg atattccttt tttcataaaa
     1981 atagtgtatc tttgcaaacc tttgtaaaag gaaatgtaaa aaatattcat taaaagatat
     2041 aaattaagaa aaaatgccaa agcaaaaaac aaactcaagt gctaaaaagc gttttaaagt
     2101 aacaggttct ggaaaaatta aaagagctaa ggcttacaaa agacacattt tgacaaagaa
     2161 aacaactaaa caaaagaaaa gattaggaaa agctggttta gtacacactt cagatatgaa
     2221 taacgctaag aaattattag cattaatcta gaaaaatata aatagtataa ggtaaggcaa
     2281 gcaccgatga ctattatcta aattattatt aaccaattaa attaaaaagc catgccacgg
     2341 tcagtaaatt cagtagctgc aaaagcaaga aaaaagaagg tctataaatt agcgaaaggc
     2401 tattttggta gaagaaaaaa tgtatggaca gtaactaaaa acgctgttga aaaaggatta
     2461 gcttacgctt atgtaggtag aaaattaaag aaaagagatt tcagaagttt atggattgta
     2521 agaattaatg cagctgtaag aaattacgga atgtcttatt ctgtattcat caacaaatta
     2581 aacaaagctg gagttgaatt aaacagaaaa gcattagctg atttagcttt aaataatcct
     2641 gaagcatttg aggctgttgt aaataaagta aaatagtttt ttataataaa taagatgaag
     2701 agccgttccc tttggaacgg tttttttatt tgaggtatat ttttattgct tttatgtttc
     2761 taatcactaa acaaaagagt ataaaattcg ttctatctat aaattatcgt tcgtaaagtg
     2821 attttcttaa aatagacttt tatatttgat taattaaatt tcttgaaaat ggaaaaaaat
     2881 acttatgatg tgattataat gggagctggg ctttctggta tcggaaccgc ttatcatcta
     2941 caaaaaaatt gcaaaggaaa gtcttatgtt attttggaaa atagacctag tttaggtggc
     3001 acttgggatt tattccgtta ccctggcatt cgttctgatt cagatatgtt tactttgggt
     3061 tatagtttta aaccttggaa ccacagtgag ccaatcgcag atggtggttt aattttggaa
     3121 tatttaaaag aagcagcgag cgaaaatgga attgataaaa acatcaaatt caatacaaaa
     3181 ataaatgaag caaagtggga tagctcaacc aatacttgga ctgtaacttg ccatgataat
     3241 aaaaccaaaa aaagtgtaag ctatacagca aaatatgtta tttcttgtct tggttattat
     3301 aattatgata agggacatac acctaaattc aatggtgtga aaaattttaa aggagagata
     3361 ttgcatccac aattttggcc agaaaattat gattattcag gtaaggaagt agtgataatt
     3421 ggaagtggag caactgcggt tactttagtg ccagccatga cagacaaagc gaagcatgta
     3481 acgatgttgc aacgttctcc aacttatatt gcaagtattc caaacagaag cttgattcct
     3541 caagcggtat tgaaaaatct accagaaaga gtgaagtatg atttgaatag agcagcacaa
     3601 attggtttgc aagtttctag ctataatctt tgtcagaatc aaccagagtt gatgaagatg
     3661 tattttagac aacatgtaaa aaaacaattg ccaaaaggtt atgacattga caaacatttc
     3721 acaccaaagt ataatccttg ggatgaaaga ttgtgtgctg ttccaaatgg tgatttattt
     3781 aaagctattc gttcaggaaa agcagaagtt gttacagacc atattgataa atttgtggaa
     3841 gatggtattt tgttagaatc aggaaaaaag ttgaaagcag atattattat tacggcaaca
     3901 ggtttagaat tgcaaatgtt gggaggtatt aaaaccttta tagatgataa agaatttaaa
     3961 gcttcagatg gaataattta caaaggagca atgattaaag atgttccgaa ttttgcattc
     4021 gttttgggtt ataccaattc ttcttggaca ttaaaatcag atatgatagg agagtatttc
     4081 tgtaaattga ttaatgaaac agagaaaaaa aataaaaagg tatttgttgt aaaatctgat
     4141 aagccaatag agaaggaacc agcattgaat tttggtgcag gttatgtaca aagagcgatt
     4201 caagcaggag ctttgccaag tcaaggaaaa gaatatcctt ggaaattaaa gcaaaactat
     4261 ctcgtggatg ctgctaattt taaagtacgt aatattcaag atgagttttt aaaattcgtg
     4321 tagtaaattt tatagaaagt aaggaggctg tccaaaatgg atagcctctt tttatttttg
     4381 tctatattga tataaaagcg ttggtgctag aattaataaa tctattgtcc aaattctcat
     4441 aattagatag ccaagtaaag caaataataa gaaaaatacc aatagaatag tatccaattt
     4501 ttttgtaaaa aatgcaataa taaatacacc ttgtgcaata attaataggc taaatgcaat
     4561 aaaagcataa taaggcgatt gaactaaaaa ttgactaatt ttataacaaa cgtggttggg
     4621 atttattaaa gacaaatcta tatgttggtt gattaagaca ttataataat gcataaaagt
     4681 ataagcagct ccgttgtgga atttatgata agctgctgat agtaaaataa aggctaaaaa
     4741 atatcttgaa aattctaaag taatttcaaa atatttctta ttaaaacaaa gcggaaaaaa
     4801 ggcgatgaat attgcaatag gcattttgtt atgcatattg gcaaatattt caacagtgct
     4861 attttgtaaa aatattaagg tcaaaaatag tatagctaaa tacttattgt cgtatttgaa
     4921 aaataagaca atagcaatta ttaaaattga tacatctata aataatccaa tataaaaatg
     4981 ttgatgaata tattgaggaa tttgtgctaa taataataac caataggtat aagctaatcc
     5041 aggtgcttgc agtggttgtc catataaatc acttatcatt cttcctgaat atagtcgata
     5101 gccatataaa aatagaaata aaattaagat gctataagca tacatttttt gattatatag
     5161 ccatcttttt tctagcatct tattggataa ttaaaaaagg atcattgtat tgataaacat
     5221 attgatggcc ttctaatctt aaatgtacac gtctgaaatc tattttttta ggaatggtgt
     5281 gtgtattgtc ttttatatac tgtaccaacc aatttttata ttcttcagga ttactcactt
     5341 gattgttgat gtgctttaca gcataatgtt taaagcattc tggcatactt ctaaaaactt
     5401 gatttgtggt tggaatagct gggtctataa attgattctt agaaagatta ttatacattt
     5461 ttgcagtatt aaataacaca gattctttcc aaatagctaa gttttcatga ttgtacagag
     5521 tatcattgat atataaaaat acgacatctt tagcactgtc taaattataa ggccgagaat
     5581 acatatcata ggtgtaccaa gggaaattta ttattctttt ttgactaaaa aaagcttgtc
     5641 caaaacaaaa taataaaaca actaaaaaga tgtatttatt ggctttatat aatcgaatga
     5701 aataccaatt tttcatgctt ttcaaaagag tgtaactttt ataaaatgaa acccaattgt
     5761 tacaactaaa ataattttta taccactcgt taataaaaaa cctacaaaag aacctatgcc
     5821 agattttaca gcaagtgtaa agttttcacc agctattaat tccccaataa tagcacccaa
     5881 aaatggacaa ataataattg aaataccacc aaaaggagct aggaaaattc caactaaaat
     5941 gcctataaaa cttcctctta tcccagcttt tgttcctcca aactttttag ttccccaaat
     6001 cggaataaaa ttatcgatta tcaatactag aatgataatt gcggcatata aaatcaataa
     6061 ccaattgggt agttcttgta ctttatttag tccgaaacgt gctaataata gtgcaataaa
     6121 agcaattggc ggcccaggaa gtggaggtag aacacaactt gctaatccta taactaataa
     6181 aataattcct atggtaatga ttagtgcaac ttccatctaa ttgtaattta taattccatt
     6241 agtttctttc aattctttgc cttcaatttc tattttccaa ccagtacttt ttgccatata
     6301 ataaatcaga ctaaaaatct ctaatttctg agcatctgct tgttgtaaaa tatcggtttc
     6361 ttgtactttt ttagctatca atgcttttgc tttttcttgc aaagatgtca atgtcttttc
     6421 atcaaaagca ttaaaaattc cttgttgcat atcataataa gaaagttgat ggtcaatagc
     6481 caaaacctca gattttggta tgttttttat tatgatagtt ttattaattt catcaacttc
     6541 aaacttcatt ttattcatat caaaaccagc taaaactttt gcatcaactt tgataattgc
     6601 tttctttcta aatcctggaa agtctatata atcataatct tcataagtca ataattcaga
     6661 aaaattagct tcaatattca ccattttcat aactttctca atcttgttga gcataattgt
     6721 gctattcact tcactcacag gcacagaggt tttccatttg tagaaaaacc aagcacagaa
     6781 tacaagtaaa acaataatta ttggtgccgc taatttattt ttcatatcaa aatttaatac
     6841 ttcaaaatta tctatattta ttgtcttatt ttaataataa aactaaattt atgatagtaa
     6901 agttgttttt aaatttttat aaatcatact aattttagaa aactaaaacc aattatacta
     6961 tttattcatt gataaactac ctatcccaaa gattttttta taatattgat tgcttgttct
     7021 aaatcatttt cttgaatcgt tagtggcgga gcaaaacgaa ttatatttcc gtgtgtcggc
     7081 tttgctaata aaccgttttc tttcatagtc atacaaattt cccaagccaa atcctctttg
     7141 ttatgctgat taatttcaat tgcattcaat aaaccttttc ctctaacttc tttgattaaa
     7201 tgatgttcaa aatttgcaaa ttcagttctg aaaatattac ccattttcat agcattttct
     7261 gctaaatttt catctataac gacttgcaaa gcagcaattg acactttaca agccaaaggg
     7321 ttgccaccaa aagtagaacc atgttcgcct ggttttatgg ttagcataac ttcattatta
     7381 gccaatacag cactgactgg caaaacacca ccgcttaaag cttttcctaa aattaaaata
     7441 tcaggtttta cagtttcata atcacaagcc aacatttttc cggttcttgc aattcctgtt
     7501 tgcacttcat cagcaatcat caatacattg tttttagaac atattgctct tacttgttgc
     7561 aaataaccat cgtctggaac cattacacca gcttcgcctt gaatgggttc aaagaaaaaa
     7621 gctgctacat ttttatcttg caaggctatt tctaaagctg gaatatcgtt gtaaggaatt
     7681 ttataaaatt cgggaacaaa aggaccgaaa ttattgtatg aacttggatc tgttgaggct
     7741 gaaactgctg caattgtacg acccataaaa ttattttctg caaaaataat cttggcttta
     7801 ttgggttcaa cgccctttac ttcatacgcc cattttctag ctaattttat tgcagtttca
     7861 ccaccttcta cgccagtatt catcatcaat actttatcat aaccaaaaat atgggtaata
     7921 aattcttcag caattcccaa agcatcatta taaaatgctc ttgaagtcaa ggttaaagtt
     7981 tgtgcttgtt cagttaatgc agcaataatt tttggatggc aatgtccttg attgactgca
     8041 gaataagcag ataaaaaatc ataatatttt ttttcatcgg tatcccaaac aaaaatatct
     8101 tttccttttg aaagtactac tggcaaagga tgataattat gagcaccaaa tctttcttct
     8161 aattgaatat aatattgagc aggcattttt gacattataa taaattttta ttgtttatct
     8221 attttgctac tcacatattc atcaactgtt gaaaatacaa aatctggata taaagttgca
     8281 aatttttcat ttttcaaacc accttgcaat ggtcgagatg ctggttgatt aagcgctgtt
     8341 gttttgagtg gaatcaaatc atagcttgca ttaggaaaat aatgtaggat acgcaatgcc
     8401 aattgcactc tgttcatata atctgtagaa gcgatattaa aaataccatt ttgtttgtca
     8461 tttaataaca aatacaaaca acgtgcaata tctgccgcat tgataggtgt tgcatattgg
     8521 tcgataggta gttttaaggt aagatgctga ttgttttgtg cttgttctac aattcttgaa
     8581 acaaagtttt tgccacgaat ttcatcacca taaacatttg taattcttaa aacaatggcc
     8641 tcttttatat tgtctaaaac ataattttca gcttccaatt tatgtttgcc ataaatagaa
     8701 attggattag gtttgtccgt ttcagcataa ggaccatttg tgccatcaaa aatataatca
     8761 gtagaaataa aaactaattt tgagtttaat tgtacagcta aatcaactat attttttgta
     8821 gataaaacag tctttttata actttcttgc tcattggttt cacaataatc gacgtgtgtt
     8881 aaagcaccac aatgtacaat cacattcggc tgaaattttt ttacatcaaa attatcagga
     8941 ttgtctaaat ttaaagtatc aaaaaaaaca gtatcatcag ttttaaatga aaaataagtt
     9001 ccgatatttt cccaattttt agcattaaaa tattccaaac aattgcctcc cactaaacct
     9061 gacgcaccaa taataaaagt tttcattctt gttgtttttt gtaaaaataa gcaattataa
     9121 aatatattct gaaactttag ttttttatga ttttgattga ctatttttga aaagatgaaa
     9181 atactaggga tttcagcata ttatcacgat tctgctatat gtttaataga agatggaaaa
     9241 atattatatg ctgcacagga agaacggttt tcgaggatta aaaacgatga aaattttcca
     9301 atcttagcca ttcagaattg tttatcagaa cttcaaattt cgctaaatga tattcgtaca
     9361 atagtttact atgaaaagcc ctttttgaag atagaaagaa ttttagaaag ttatttagat
     9421 gttgcaccac atggatttca atctttttta caagcaatgc atgtttggac aaaagagaaa
     9481 ttatttatca aacaaaatat ctataaagaa ctaaaaaaaa tagaccaaaa tttcaataaa
     9541 aagaatattt taatgctgtt ttccgaacat catttgtcgc atttagcttc agctttt
//
LOCUS       DAOQCW010000100         9487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_2733_length_9487_cov_5.387511, whole genome shotgun sequence.
ACCESSION   DAOQCW010000100 DAOQCW010000000
VERSION     DAOQCW010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9487
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2733_length_9487_cov_5.387511"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..801)
                     /locus_tag="PLS16_09025"
     CDS             complement(91..801)
                     /locus_tag="PLS16_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNJ61263.1"
                     /translation="MHHRENDKLDCELCSNKQKSVFCKTSCENMEEIKATKICSTYKK
                     GEIIFKEGSFASGVYCINSGKIKVSMLGDEGREQIVRLAKAGDIIGYKALLSSVRYSA
                     SAVAIDDSNVCFIPREAFIHTLKKDALLSFEMMRLLSDELRNAEGKITHLAQKPVRER
                     MAETILFLKETYGIDEEENINVALSREEIANIVGTATETAIRLLSEFNKDKLIELSGK
                     KIKIINTEKLSRVANLYD"
     gene            complement(849..1283)
                     /gene="rpiB"
                     /locus_tag="PLS16_09030"
     CDS             complement(849..1283)
                     /gene="rpiB"
                     /locus_tag="PLS16_09030"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNJ61264.1"
                     /translation="MNFPSKIVIGSDHAGFEYKTEIIEFLRKINISIIDVGTNSEASC
                     DYPDYAHALAEKIENKEAEMGILLCGSANGVAMAANKHQAIRAALCWENEIASLARLH
                     NDANVLCLPARYVSIESAFEMVNTFFTTDFEGGRHQKRVDKI"
     gene            complement(1295..3919)
                     /gene="alaS"
                     /locus_tag="PLS16_09035"
     CDS             complement(1295..3919)
                     /gene="alaS"
                     /locus_tag="PLS16_09035"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNJ61265.1"
                     /translation="MNQLSASEIRNAFLDFFKSKQHQIVASAPIVVKNDPTLMFINSG
                     MAPFKDYFLGIQQPKFTRIADTQKCLRVSGKHNDLEEVGVDTYHHTMFEMLGNWSFGD
                     YFKKEAIAWSWEFLTEVLKLEKDRLYVTIFEGNEAENIPKDEEAYNEWKKVIAEDRIL
                     LGNKKDNFWEMGDTGPCGPCTEIHVDCRSDEERNQTDGATLVNNDHPQVIEIWNNVFI
                     QFNRKKDQSLELLPSKHVDTGMGFERLVRVIQGKQSNYDTDIFTGTIQAIEKIVDKKY
                     DGSDSKQAIAFRVLSDHIRAVAFTIGDGQLPSNTGAGYVIRRILRRAVRYYYSHLDRK
                     EPLLHLLVPVLAKQFELVFPELKAQENFVAKVIFEEETAFLKTLDNGLKRLDHIINES
                     NQIIDGKAAFELLDTYGFPIDLTNLIASEHHIKVDEVGFNEALQQQKERSRAASELSV
                     DDWVVLSDGKSTFVGYDTLESTTKVLKYRKIKSKDKEQYQIVLEQTPFYAESGGQVGD
                     IGNLYIKGNLIQIVDTKKENDLIVHFANEIIDDLEGEITAEVNEQNRQETTIHHTATH
                     LLQAALQQVIGKHIQQKGSLNNSEYLRFDFSHFAKLTDDEIAKVENLVNEKIRQNIPV
                     VIKEMPKEEALKLGAMALFGEKYGDIVRVVIIDEDYSVEFCGGTHAGYTGELGLFKII
                     SESAVAAGVRRIEALSGLKALRNVNQKIETLEAIHSELNNPKDILKAIQNLLDENSNL
                     KKEIEKYQNEKVEQIKQELKNKIVQHGSINLLIEKIDGVTADGLKKIQFDLKNEVENL
                     VFGAVAEINGKPFISIIINDAISKHNNLHAGNMVKQLAKLIKGGGGGQPFYATAGGSD
                     LSGLNAVLDEMSIIFKVK"
     gene            complement(3976..4593)
                     /locus_tag="PLS16_09040"
     CDS             complement(3976..4593)
                     /locus_tag="PLS16_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61266.1"
                     /translation="MTHLFILFVCVISAIACNNNNNHAKQQNISDGIDSIQTISAASN
                     KDSLPTELANNEQIKVIKSNFSRINSIQKWTKIKTLELNETTEGGEAKFYFLNGKIEK
                     IVAVYYGEMFQKVTEFYLQDAQLSFVFEKLTTYKQAIYDNETPFDVEKTEEFRSYFKK
                     DNLIYRIESQPTNLPFSKAYFGKEQERLIQEFKQLNEMNANNSNN"
     gene            complement(4637..5311)
                     /locus_tag="PLS16_09045"
     CDS             complement(4637..5311)
                     /locus_tag="PLS16_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61267.1"
                     /translation="MKQLVFFLSFFMSVLSLLAQEEIYEAPRPKTPVYIDNREELLND
                     EETPTKQKRNERYADEDSVQKKFDPSKLRIGGNFGLQFGNYTFVNLSPTVGYIFLKDR
                     LEVGAGPIFIYQSVRYSNNYRQSYLLYGGDFYTRGYIWKGIFAQAQYDLINKESYYKN
                     ERINVHHLLIGGGYSTPIGDVGSFYASLMYNVLNNSESVYRGTFGDFPLIMNVGFGFG
                     INRRNK"
     gene            5362..7521
                     /locus_tag="PLS16_09050"
     CDS             5362..7521
                     /locus_tag="PLS16_09050"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNJ61268.1"
                     /translation="MGKIKLNILLSVLFIFLFYSINAQLNLEDIFLKNKYSPDALDDI
                     VFLNHKENYAALEDYTSTQKIITYNPTGKQESVINLKEIFKNNDEFEQTISKFEISNS
                     DKYYLLESNPNYQYRYSYTANYYLLEGNQLSALPEQNIELPTFSAEDNKIIFIKDNNL
                     YYYDIKNNINRQITFDGVKNKIINGKSDWVYEEEFSLTKAYCINQQSNKIAYLKFDES
                     QVKEFTMQYYNGKKYPQNFTYKYPKFGENNSKVSLWYYDVKKKKNYSIPINSSYEYLP
                     RIYFGADGNTIYYMLFNRLQNQLKIETYNIQTKKTKTVYSENSTTYINLPQFILLPDQ
                     SFFITSQKENYNQIYHYDAFGKLIKKVTKDDFNVQNIIALDIQNKLIYFETNKDKISE
                     KQIYSINYSTDISQALTFERGINEVFFAPQMTYYIHRYSNDTTPTQVKILYTNQTSST
                     ILIDNQKVKSSISNLPKKEFFSFKTEDGTALESWIIKPTRLDTTQKYPLLLYVYGGPG
                     NQEVLNNYGRNDSKWFKYLAEQGYVVACVDGRGSGGKTANFEHIIYKNLGKYETKDQI
                     SAAKYFKSLDFIDSNRIGIMGWSYGGFMALNCLEEGNEFFKIAISIAPVTNWIWYDNI
                     YTERYMQTPLENKEGYLSYNPILNASKIKGKLLLVHGTADDNVQIQHTYELMSVLNNK
                     NIPYESLIYPDKNHSISGGNTRFDLYQKLTKFIVKNL"
     gene            complement(7508..8005)
                     /gene="folK"
                     /locus_tag="PLS16_09055"
     CDS             complement(7508..8005)
                     /gene="folK"
                     /locus_tag="PLS16_09055"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNJ61269.1"
                     /translation="MHKILILLGSNKGYRYKHLLLAKYFIQNEIGRINSASNIYETEP
                     WQVLGKQSAYLNQCLEVQSTLGIFQLLNATQKIEKRLGRNNKNQNQSRTIDIDILFYD
                     DILFETKKLIIPHPRLHLRKFTLLPLVEIAPDFIHPKMNQRIQELLKNVQDNAEVKRY
                     IPKGF"
     gene            8060..>9487
                     /gene="sppA"
                     /locus_tag="PLS16_09060"
     CDS             8060..>9487
                     /gene="sppA"
                     /locus_tag="PLS16_09060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNJ61270.1"
                     /translation="MKSFFKIIFGSCLGVILSFVVIFFILMGAASSISPSSSKKDKAI
                     KSNSILSLKLEGEITDRTYEASFMNFSPFGSNAASSIGLFDIEKVLNKAKTDDNIKIL
                     ALDIDKLSMGIASTYQFIQMLEDFKKSNKPVYLYSNGASQKTILVGAYADKVYLNPIA
                     SCEFDGFVLENMFYTGLLEKVGVQPRIFYAGEFKSATEPFRLKANSPENILQLTELLQ
                     DVSTKYYTSLAQKLKQNPDSIIAYANQLKITQAADAKKYGLVSDLKYEDEFFDELKTK
                     LGYKKDDKVNFVSYKTYKESIIPEKNSSDAQNQIAVLFAEGEITDGKGEQGIIASENF
                     LNEIRGIQAKVKDKKVKALVLRVNSPGGSAYASEQIWRELSLLKKEIPIVVSFGDVAA
                     SGGYYISCASNKIFAQDNTITGSIGVFGMLFNMQNLLNNKLGITTDAVKTSPYADFGN
                     STRDWDETENNAMQRDIDFIYTLFKK"
BASE COUNT         3312 a         1535 c         1241 g         3399 t
ORIGIN      
        1 atggttttaa ggtcgtaaat ctaaatggtg gttatggatt atggtcgatg atatatccaa
       61 attaagctag aaaaaatcta caataaaaca ttaatcatat aaattagcta cacgagataa
      121 tttttctgta ttaatgattt ttatcttttt gccagataat tcaataagtt tatctttatt
      181 aaattctgaa agcaaccgta ttgcagtttc agtggctgta cctacaatat tagcgatttc
      241 ttcacgagac aaagcaacat tgatattctc ttcttcatca atgccataag tctctttgag
      301 aaataaaatt gtttctgcca tacgttcacg aactggtttt tgtgccaagt gtgtaatttt
      361 tccttcagca tttctcagtt catcagacaa taatcgcatc atttcaaaag aaagtaaggc
      421 atcttttttt aatgtatgaa taaaagcttc tctcggaata aaacacacat tactatcatc
      481 aatagctact gctgaagcac tatatctaac tgaagaaagc aaagctttat aaccaattat
      541 atctcctgct tttgccaatc ttacaatttg ttctctacct tcatcaccca acatggaaac
      601 ttttatcttg cctgaattta tacaataaac acctgacgca aaactacctt ccttaaagat
      661 aatttctccc tttttgtaag ttgaacaaat tttagttgcc ttaatttctt ccatattctc
      721 acaagaagtc ttacaaaaaa cagacttttg cttattagaa cataattcgc aatctaattt
      781 atcattttct ctatgatgca tatcgttaca aagttataaa ataatcaatc aataaacaat
      841 tacaacaact aaatcttatc tactcttttt tgatgtctgc caccttcaaa atctgttgta
      901 aaaaaagtat taaccatttc aaacgcagat tcaatactaa catatcttgc tggtaaacaa
      961 agtacatttg catcattatg taaccttgcc aaactggcta tttcattttc ccaacataaa
     1021 gcggcacgta ttgcctgatg tttattagct gccatagcta caccgtttgc acttccacac
     1081 aataaaattc ccatttcggc ttctttattt tcaatttttt ctgccaaagc atgagcataa
     1141 tctggataat cacatgaagc ctcagaattt gttccaacat caattatgct tatatttatt
     1201 tttcttaaaa attcaattat ttctgtctta tattcaaatc cagcgtggtc gctaccaatt
     1261 actatcttac taggaaaatt catattttag ttatttactt tactttaaaa attatactca
     1321 tttcatctaa tacggcattc aaaccactta aatctgaacc gccagctgtt gcataaaaag
     1381 gctgacctcc gcccccacct ttaatcaatt ttgccaattg tttaaccata tttccagcgt
     1441 gtaaattatt atgcttacta attgcgtcat taataataat cgaaataaat ggcttaccat
     1501 ttatttctgc caccgcacca aatactaagt tttcaacttc attttttaag tcaaattgaa
     1561 ttttctttaa gccatcagca gttacaccat caattttttc aatcaataaa tttattgaac
     1621 cgtgttgtac aattttattt tttaattctt gtttaatttg ttctactttt tcattttgat
     1681 atttctcaat ttctttcttt aaattgctat tttcatctag caaattctga attgctttta
     1741 aaatatcttt gggattattt agctctgaat gtatagcctc caatgtttca atcttttgat
     1801 ttacatttct caatgctttc aaaccactta gtgcttcaat tcttctaaca ccagctgcca
     1861 cagcactttc agagataatt ttaaacaatc ctaattctcc agtataacca gcatgtgtac
     1921 caccacaaaa ttctactgaa taatcttcat ctataattac tacacgaaca atatcgccgt
     1981 atttttcacc aaacaatgcc attgcaccta atttcaaggc ttcttctttt ggcatttctt
     2041 ttatgactac aggaatattt tgacgtattt tttcatttac taaattctca actttagcaa
     2101 tttcatcatc cgttaattta gcaaaatgtg aaaagtcgaa acgtaaatat tccgaattat
     2161 tcaaagagcc tttctgttga atatgtttgc caattacttg ttgcaaagct gcttgcaata
     2221 aatgcgttgc cgtatgatgg atagtcgttt cttgtctgtt ttgttcattg acttcagctg
     2281 tgatttcgcc ttctaaatca tcaataattt cattggcaaa gtgtacgatt aagtcgttct
     2341 ctttttttgt atctacaatc tgaattagat ttcctttgat gtataaattt ccaatatctc
     2401 caacttgtcc accactttct gcataaaatg gcgtttgctc caaaacaatt tgatattgtt
     2461 ctttatcttt agatttgatt ttacgatatt tcaatacttt agtcgttgat tctaaagtat
     2521 cataacctac gaaagtagat tttccatcac ttaaaacgac ccaatcatca acactcaatt
     2581 cagatgccgc acgacttctt tctttttgtt gttgcaaagc ctcattaaat ccaacttcat
     2641 caactttgat atgatgttca gaagcaatta aattggttaa atcaatagga aaaccataag
     2701 tatccaataa ttcaaaagca gctttcccat caataatttg attactttca ttgataatat
     2761 ggtctaatct ttttaagcca ttatctaaag tttttagaaa tgcagtttct tcttcaaaaa
     2821 ttacttttgc aacaaaattt tcttgagctt ttagttctgg aaatactagt tcaaactgct
     2881 ttgctaaaac tggaactaat aaatgtaaca aaggctcttt tcggtctaag tgtgaataat
     2941 aataacgtac agcacgtctt aaaattctac gaataacata accagctcct gtattgctcg
     3001 gtaattgtcc atctcctatt gtaaatgcta cggcacgaat atggtcagat aaaacacgaa
     3061 aagctattgc ttgtttacta tctgaaccat catatttttt atccactatt ttttcaatag
     3121 cttgaattgt tcctgtgaaa atatctgtat cgtaatttga ttgtttgcct tgaattacac
     3181 gaactaaacg ttcaaaaccc attcctgtat caacgtgttt ggacggcaac aactctaaac
     3241 tttgatcttt ttttctatta aattggataa atacattatt ccatatttca atcacttgag
     3301 ggtggtcgtt attgactaaa gtagctccat cagtttggtt tcgctcttca tcagaacggc
     3361 aatccacatg aatttcagta catggtccgc aaggtccagt atcgcccatt tcccaaaaat
     3421 tatctttttt attgcctaat aaaattctgt cttcagcaat aacttttttc cattcattgt
     3481 atgcttcttc atcttttgga atattttcag cttcattgcc ttcaaaaatt gtaacataca
     3541 atctatcttt ttctaatttc aaaacttcag tcagaaattc ccaactccaa gcaatggctt
     3601 cttttttaaa ataatcgcca aaactccaat tgcccaacat ctcaaacatc gtatgatgat
     3661 aggtatctac gcccacttct tctaaatcat tatgttttcc tgaaactctt aaacattttt
     3721 gggtatctgc aattcttgta aatttaggtt gctgaatccc taagaaataa tctttaaaag
     3781 gagccattcc tgaatttata aacatcaaag ttgggtcatt ttttactaca attggagctg
     3841 atgccacaat ctgatgttgt ttacttttaa aaaaatctaa aaaagcattt ctgatttctg
     3901 atgcagataa ttgattcata gcacaaagat aaattatttc aaccaatcaa taagtgcatt
     3961 ttctataaga gaatattaat tattcgagtt gtttgcattc atttcattta actgcttaaa
     4021 ctcttgaatc aaacgctctt gttctttacc aaaataggct ttggaaaatg gtaaattggt
     4081 aggctgactt tctattctat aaattaaatt atctttttta aaatagcttc taaattcttc
     4141 agtcttttca acatcaaaag gtgtttcatt atcataaatt gcttgcttat aagttgtcaa
     4201 tttttcaaaa acaaaagaca attgagcatc ttgtaaataa aattctgtta ctttttgaaa
     4261 catttcgcca taataaacag caacaatttt ttcaattttt ccattgagaa aataaaactt
     4321 tgcttctcca ccttcggtag tttcattgag ttctaaagtt ttaatttttg tccatttttg
     4381 aattgaatta attctgctaa aatttgattt tataactttt atttgttcat tattagctag
     4441 ttctgttggc aaactatcct tattggaagc tgcacttatt gtttgtatag aatcaattcc
     4501 atcacttata ttttgctgct tagcatgatt gttattatta ttacaagcaa tagcactaat
     4561 tacacaaaca aataaaataa ataaatgagt cattattctc attataataa ttttcctata
     4621 aagttactta aaaaaattat ttatttcttc ggttgatacc aaatccaaaa cctacattca
     4681 taataagtgg aaaatcgcca aaggtacctc gataaactga ctcgctatta ttcaatacat
     4741 tatacattaa acttgcataa aatgaaccaa catcgccaat cggtgtagaa tagccaccgc
     4801 caattaaaag atgatgtaca ttgattcttt catttttata ataggattct ttattaatta
     4861 aatcatattg tgcttgagca aaaattcctt tccaaatata acctctagta taaaaatcgc
     4921 caccatataa caaatagctt tgtctataat tattactata acgaacactt tgatatataa
     4981 agataggacc agcacccact tctaatctgt ctttaaggaa aatataacca actgttggtg
     5041 ataaattgac aaaagtataa tttccaaatt gtaatccaaa gttgcctcct attctcaatt
     5101 ttgatggatc aaatttcttt tgaacactat cttcgtcagc atatctttca ttgcgttttt
     5161 gcttagttgg cgtttcttca tcattcaaca attcttctct attatcaata taaacaggtg
     5221 tttttggacg gggagcttca taaatttctt cttgagccaa taggctcaat acactcataa
     5281 aaaaagataa aaagaatact aactgtttca ttatgccaaa ttaattatta aaatgtattt
     5341 ttattcctaa ttattattaa gatgggcaaa attaagctaa atattttatt atcagtatta
     5401 tttatatttt tattttatag tatcaatgca caactcaacc tcgaagatat ttttttgaaa
     5461 aataaatatt cgccagatgc attagatgat atcgtctttt taaatcacaa agaaaattat
     5521 gctgcactag aggattacac ttcaactcag aaaattatta cttataatcc tactggaaaa
     5581 caagaatcag tcatcaattt aaaagaaatt ttcaaaaata atgatgaatt tgaacaaaca
     5641 atatcaaaat ttgaaatatc caattctgat aaatattatt tattagaaag taacccaaat
     5701 tatcaatatc ggtattctta tactgcaaat tattatttat tagaaggtaa tcaactcagt
     5761 gctttaccag aacaaaatat tgaattacct acattttctg cagaagacaa taaaattata
     5821 tttataaaag ataataattt atactattac gatattaaaa ataatataaa cagacaaatc
     5881 acttttgatg gtgttaaaaa taaaatcatt aatggcaaaa gtgattgggt atatgaagag
     5941 gaatttagtt tgacgaaagc gtattgcatt aatcaacaaa gtaataaaat tgcctatttg
     6001 aaatttgatg aaagtcaggt aaaagaattt acaatgcaat attataatgg caaaaaatat
     6061 cctcaaaatt ttacctataa atatccaaaa tttggcgaaa ataatagtaa agtttcactt
     6121 tggtattatg atgtaaaaaa gaaaaaaaac tattctattc ctattaactc atcttatgaa
     6181 tacctaccaa gaatatattt tggagctgat ggaaatacta tatattatat gctttttaat
     6241 aggttgcaaa atcaattaaa aattgaaaca tacaacattc aaacgaaaaa aacgaaaaca
     6301 gtatattctg aaaacagtac aacatacata aatttaccac aatttatttt actgcctgac
     6361 caatcgtttt tcattacctc acaaaaagaa aattacaatc aaatttatca ctatgatgct
     6421 tttggaaaat tgataaaaaa agttacaaaa gacgatttta atgtgcaaaa tattatagca
     6481 ttagacatac aaaacaaact catttatttt gaaactaata aagataaaat atctgaaaaa
     6541 caaatttata gtatcaatta ttcaactgat attagccaag cactaacttt tgaaagaggt
     6601 attaatgaag tattttttgc tccacaaatg acctattata ttcatcgtta cagtaatgat
     6661 acgacaccaa cacaagttaa aatattatat accaatcaaa catcatctac tattttgatt
     6721 gataatcaaa aagtaaaaag ttctatttca aatctaccta aaaaagaatt ttttagcttt
     6781 aaaactgaag atggaactgc gttggaatca tggataatta aacctacaag attagataca
     6841 actcaaaaat atcctctatt actttatgtt tatggtggtc ctggcaatca agaagttttg
     6901 aataattatg gacgaaatga tagcaaatgg tttaaatact tagcagaaca aggttatgtt
     6961 gttgcctgtg ttgatggtcg tggaagtggc ggaaaaactg caaattttga gcatattatt
     7021 tataaaaatc tgggcaaata tgaaaccaaa gaccaaatta gtgctgcaaa atacttcaaa
     7081 tcattagatt ttatagatag caatagaatt ggcataatgg gttggagtta tggtggtttt
     7141 atggctttaa attgcttgga agaaggcaac gaatttttta aaattgctat tagtattgca
     7201 cctgttacca attggatttg gtatgataat atatatacag aaagatacat gcaaacacct
     7261 ttagaaaata aagaaggcta tttaagttac aacccaattt tgaatgcttc aaaaataaaa
     7321 ggaaagcttt tgttggtaca tggaactgct gacgataatg ttcaaataca acacacttat
     7381 gaattgatgt ccgttttaaa caataaaaat attccttatg aatctttaat ttatccagac
     7441 aaaaaccata gtatttcagg cggcaatact cgatttgatt tgtatcaaaa acttacaaaa
     7501 tttattgtta aaaaccttta ggaatatatc ttttgacttc agcgttatct tgtacattct
     7561 tcaataattc ttgaatcctt tgattcattt taggatgaat aaaatcagga gcaatttcaa
     7621 ctaaaggcaa taaggtaaat tttcgcaaat gcaaacgagg gtgtggaatg attaattttt
     7681 tagtttcaaa caaaatatca tcataaaata aaatatcaat atcaatagtt cggctttgat
     7741 tttgattctt attatttctc cctaatcgtt tttcaatttt ttgagtagca tttagtaatt
     7801 gaaaaatgcc taaggtactt tgtacttcga gacattgatt caaatatgca gattgcttac
     7861 ctaaaacttg ccaaggttct gtttcataaa tattagaagc ggaatttatt cttcctattt
     7921 cattttggat aaaatatttt gccaacaata aatgtttgta tctataacct ttattgctac
     7981 ctaacaaaat taatatttta tgcatagtgc aatattaaaa aataaattac ttttgttgtt
     8041 ataatattcg tttttaatta tgaaatcatt ttttaaaatt atttttggct cgtgtttagg
     8101 tgtcatctta tcttttgtcg ttatattctt tattttaatg ggtgctgcat caagcatttc
     8161 gccatctagt tctaaaaaag acaaagcaat aaaaagcaat tcaattttat cgctaaaatt
     8221 agaaggcgaa atcacggaca gaacttatga agcttcattt atgaacttct ctccttttgg
     8281 ctcaaatgcc gcaagttcaa taggtttgtt tgatattgaa aaggttctta ataaagcaaa
     8341 aaccgatgat aatattaaaa ttttagcttt agatattgac aaattatcga tgggcattgc
     8401 ttccacttat caatttattc aaatgctaga agatttcaaa aaatcgaata agccagttta
     8461 cttatatagc aatggtgcaa gtcaaaaaac tattttagtt ggtgcttatg ctgataaagt
     8521 atatttgaat ccaattgcca gttgcgaatt tgatggcttt gttttagaaa atatgtttta
     8581 tacaggtttg ttagaaaaag tgggtgtaca accaagaatt ttttatgctg gagaatttaa
     8641 aagtgctacc gaaccattta gattaaaagc caatagccct gaaaatatat tacaattaac
     8701 tgaattattg caagatgtta gtacaaaata ttatacatct cttgctcaaa agctgaaaca
     8761 aaatcctgat agcattatag cttatgctaa tcaattaaaa attacacaag cagctgatgc
     8821 aaaaaaatat ggtttagtaa gtgatttaaa atatgaagat gaattttttg atgaattaaa
     8881 gactaaatta ggttataaaa aagatgataa agtaaatttt gtttcttata aaacatataa
     8941 agaaagtata attcctgaaa agaatagtag tgatgctcaa aatcaaattg cagtattatt
     9001 tgctgaagga gaaattaccg atggtaaagg cgagcaaggt attattgcaa gtgagaattt
     9061 tttaaatgaa attcgtggta ttcaagctaa agtgaaagat aaaaaagtaa aagctttggt
     9121 tttgcgtgtt aattctccag gtggaagtgc ttatgcttct gaacaaatat ggagagaact
     9181 cagtttactt aaaaaggaaa ttcctatcgt tgtttcattt ggtgatgtag ccgcaagtgg
     9241 tggctattat atttcttgtg ccagcaataa aatatttgct caagacaata caatcactgg
     9301 ttctattggt gtttttggaa tgttatttaa tatgcaaaac ttattaaata ataaattagg
     9361 aattacgaca gatgctgtaa aaacaagtcc gtatgcagat tttggtaatt ctacaagaga
     9421 ttgggatgaa actgaaaaca atgctatgca aagagatatt gattttatct atactttatt
     9481 caaaaaa
//
LOCUS       DAOQCW010000101         9476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_2739_length_9476_cov_4.447192, whole genome shotgun sequence.
ACCESSION   DAOQCW010000101 DAOQCW010000000
VERSION     DAOQCW010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9476
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2739_length_9476_cov_4.447192"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PLS16_09065"
     CDS             <1..302
                     /locus_tag="PLS16_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61271.1"
                     /translation="VKIENFIKDVKSLFGAMVNTTLLKKTLDILDHVTHTMESKPHIF
                     PQKEVEGIKLGRSLLERGYNIFSKYDEATKQAIVDFIYKNEMWYIDSVYKKKENR"
     gene            complement(389..1111)
                     /locus_tag="PLS16_09070"
     CDS             complement(389..1111)
                     /locus_tag="PLS16_09070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNJ61272.1"
                     /translation="MSLEDFKKKKPYYQRSWTQVNASNSWSMFRIMSEFVDAYEKMDK
                     IGPCISIFGSARTKPDNIFYQKSITIAERLVEEGYGVITGGGPGIMEAGNKGARNKEG
                     KSVGLNIELPHEQGHNEYIDFDKIITHHYFFVRKVVFVKYAQGFVYLPGGFGTLDELF
                     EVLTLVQTKKIDRVPIVLFGSDYWGGLLEWIKHTLLEQYQNINKEDLNLFLLTDDVEE
                     AIHYINNFYDTDHSRDLKPNFS"
     gene            complement(1142..2029)
                     /locus_tag="PLS16_09075"
     CDS             complement(1142..2029)
                     /locus_tag="PLS16_09075"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNJ61273.1"
                     /translation="MRSVQSLVAQGIIRAFREVLFIHKTETNTHRINFERVSNIIKFP
                     RFVKMEETTYLGLDIAWFIPKKLDKKRIVLYLHGGGYCVGSYHSHRALIARIARETQY
                     KTIAINYRMAPENPFPAAVEDAVAIYKQLLADGYQEIIVSGDSAGGGLSLVLTYQIKK
                     LGLQLPKGLILLSPWTDLTMSGESIQTKVQDDALIAPNLIELFANKYIGEDSATNPLI
                     SPLFLEFENFPPTLIQVGSFEVLLDDSTRLAKKMHDAGVQVDFEIYEKQMHVFQFLAG
                     IVPEANRAIEKMGKFTKIL"
     gene            complement(2090..2977)
                     /locus_tag="PLS16_09080"
     CDS             complement(2090..2977)
                     /locus_tag="PLS16_09080"
                     /inference="COORDINATES: protein motif:HMM:NF021509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HNJ61274.1"
                     /translation="MFENKILRKIKSLNPEKDHWEIVRLALFYEFPWDAQRALELALY
                     KTYAVPSISKILHQTRELENNTAKRYDDTDILLSLIVEHGLKDNKGKEALERMNWIHA
                     QFKISNDDYIYVLTTFVFDSKRWINEFAYRKLSRNEELALYYVWLEIGAAMGIKDIPN
                     TYEKLEQFHIDYERTNFKYTENNVLLANATENMFLSWYVPESLFETFRPFLHALMEPH
                     LLQAFNRTEPPKQIKDFVRKALRTRSFLESLIPNRQPFYRSTMAKWHHYKDGYKMEEI
                     GPEKIISKCPFPHHLSKEK"
     gene            complement(3115..5628)
                     /gene="topA"
                     /locus_tag="PLS16_09085"
     CDS             complement(3115..5628)
                     /gene="topA"
                     /locus_tag="PLS16_09085"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNJ61275.1"
                     /translation="MTKNLLIVESPAKAKTIEKILGKDFTVKSCFGHIRDLAKKDMGI
                     DIANNFQPHYVISEDKEKVVKELQKLAKGSEVWLATDEDREGEAISWHLCEVLNLDKD
                     TTKRIVFNEITAPAIKKAVENPRTLDTNLFYAQQARRVLDRVVGFELSPVLWKKLGRR
                     TLSAGRVQSVAVRLVVEREREINKFETESFYKVVAYFISDKKVFKAELSEKYSEIKSA
                     EQFLEDCKQAIFTVKDIQTKPTQKGPAAPFTTSTLQQEASRKLGFSVSRTMQVAQKLY
                     EAGKITYMRTDSPSLSDLALNSIQAYINKEFGKDYSNRKQYATKNASAQEAHEAIRPT
                     YIENTSEGSNRDEEKLYQLIWKRTIASQMSNARLEKTIAKIDISNRKELFVAEGEVLK
                     FEGFLKVYIESTDDEEEDEAGMLPALNINQILDYTKIEARERFSQAPARFTEASLIKK
                     LEELGIGRPSTYAPTISTIQNREYVIRGELEGQPRAYQKLELSKGNILKETLSENVGA
                     QKGKLIPTDIGILVTDYLLDNFPKIMDYSFTADMEKDFDLIAEGNKEWSKIIQQFYKP
                     FHNDIEASAESTTFVTGERELGVEPESNKLITARLGRFGPMVQIGSADDEEKPRYAKL
                     KPGQSIETISLDEALDLFKLPLTLGIYKEQEISVNNGRFGPYVKFGEQFVSLPKGEDP
                     INVSLERAIEIIKNKQVEDAPIAYYDKKPITKGKGRFGPFIKWNDMYINIPRAYNFDT
                     INEKECEELILKKIDKEANRYILEFPEEKISVENGRWGPFLRFNKKMLKLTGKKYTSD
                     EAANLSLEDLKKMIEAQVPGAFEKKPKKAPAKKAATKKK"
     gene            complement(5781..6965)
                     /locus_tag="PLS16_09090"
     CDS             complement(5781..6965)
                     /locus_tag="PLS16_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HNJ61276.1"
                     /translation="MTKLQFPENFIWGSSTAAYQIEKAGEHDWKGFKSVDGSIFDNCS
                     MHDSHREEDLELICQVSNGYRMSHDWSKLQKAPFAEFDKVEVEKYLVFMQELKNRGMH
                     IMLVLHHFTHPKWFVETGSWEKGDNYKMWVDFAKKSVDTFGHLVDTWNTFNEPNVYVS
                     NGYILGQFPPFYKGQLFKARRVLKAMEKAHNEVYDYIHKKIPNAQVGISHNTVKFVGE
                     VFPGHLLAKLFDWWFMDYCHKHFQKVDFQGLSYYAKMPFRPLPIDNINNPGVLAKLGR
                     RHDMMWEYDPKGFYEVFHRYYKQNGRPIIITESGVCTEDPQFRIESIKEYLSWVHQAQ
                     QDGIKIKGYFHWSTIDNHEWNIGLKYRFGLVRVNFETGERTMTDAGRFMKKVATENAV
                     EV"
     assembly_gap    7128..7227
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7228..7760
                     /locus_tag="PLS16_09095"
     CDS             <7228..7760
                     /locus_tag="PLS16_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61277.1"
                     /translation="TNTKNSMRVRIPMNRNLVYISILILTTFFWCLGLFMLIDLFLKL
                     HNFYYKLFLFFGFLVWNGIGLAGVSFFMWIFFGRERIVVNKDYFITEKPLIFFFRRNI
                     YNINEVSKIRIDKETFKINRNGTWVENQRTVLKFDTPKKHVVFARNIAVSDAEYILLL
                     LAKCKYLKAEQFAIVM"
     gene            7824..>9476
                     /locus_tag="PLS16_09100"
     CDS             7824..>9476
                     /locus_tag="PLS16_09100"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNJ61278.1"
                     /translation="MRILFILFFSIYHFISFAQTPDKALKYYNSAIEYGQTAQFTEAI
                     EELKKAIQIYPDYLDAKIFLGEIYFSTKKYTDCIAILENAKENIKTIYPSIYQTKMYW
                     LLADAYLLDKNFEKCIINAQMYLNQNKKTEYGIQKANRLIATANFSKQAIANPVNFHP
                     TNIGESINSAFDDYFPVLTPDAKQLFFTRKINGQEDIYYSDKINNTWTKAQLLDDKVN
                     TPNYNEGSQSISANGNLLFFTTCNRPNVFGSCDIFYTYFDGKNWMQANGINKPINTSF
                     WDAQPSYAADGRALYFSSDRQNGVGGKDIWVSYLDDQLKWSEPKNLGDQINTPYDEQT
                     PFIHPDGKTLYFISNGHVGMGMNDIFMSKLENGVWSKPVNLGYPINTEKDEMGLFVST
                     DGSKAYFASNRAEGFGKLDIYEFDLPQAFKPQATTYVKVFVKNALNKNPLYSQYSIID
                     INTNEVIIKGNTNSEGNFTVCLTANRDYALIVQKDGYLLQTENFSFKEGTIFQPYTLD
                     IYLQAIEKNSQVVLNNIFYESNSATINPKSYTELDKLFEFLTK"
BASE COUNT         3130 a         1595 c         1253 g         3398 t
ORIGIN      
        1 gagttaaaat tgaaaatttt attaaagatg taaagagttt gtttggtgcg atggtaaata
       61 ctactttatt aaagaaaact ttagatattt tagatcatgt tacacacaca atggaaagca
      121 aaccacatat tttccctcaa aaagaagtag aaggtataaa attaggtaga agtttattgg
      181 aaagaggtta caatattttt tccaaatatg atgaagctac aaagcaagca attgttgatt
      241 ttatttataa aaatgaaatg tggtatattg attctgtcta taaaaaaaag gaaaatagat
      301 aagagttttt ctaaaagtac aactgtaatt tacggtaatc taacgtaaat tacagttgtc
      361 tttaagcttc aattcataaa gaaaatgtct aagagaaatt tggtttcaaa tctctactat
      421 ggtcagtatc gtagaaatta ttgatatagt gaatggcttc ttcaacatca tctgtaagta
      481 aaaataaatt caaatcctct ttattgatat tttgatattg ttctaaaagt gtatgtttta
      541 tccattctaa taatccgccc caataatcag aaccaaaaag aacgattggg actctatcaa
      601 tttttttagt ttgtactaaa gttaatactt caaataattc atctaaagta ccaaagccac
      661 ctggcaaata cacgaaacct tgagcatact ttacaaacac aacttttctt acaaagaaat
      721 aatgatgcgt tataatttta tcaaaatcta tgtactcatt gtgtccttgt tcatgtggta
      781 attcaatatt taatcctact gattttcctt ctttgtttct agctccttta tttccagctt
      841 ccataattcc aggtccaccg ccagttatca caccataacc ctcttctact aacctttccg
      901 caatagttat tgatttttga taaaatatat tatcaggttt ggtacgtgca gaaccaaata
      961 ttgaaataca cgggcctatc ttatccattt tctcataagc atcaacaaac tcgctcataa
     1021 ttcggaacat actccaagaa tttgatgcat ttacttgtgt ccaacttctt tggtaatacg
     1081 gctttttctt tttaaaatct tctaatgaca taataatata ttaattaaca aataaattaa
     1141 actaaagaat ttttgtgaat tttcccattt tctcaatagc tctattggct tcaggaacaa
     1201 tacccgccaa gaactgaaaa acatgcattt gtttttcata gatttcaaaa tcaacctgta
     1261 ctccagcatc atgcattttt ttagctaatc gagtggaatc atctaataat acttcaaaac
     1321 ttcctacttg aattaaagta ggtggaaaat tctcaaactc taaaaacaaa ggtgatatta
     1381 atggattagt tgcactatcc tcaccaatat atttattggc aaacaattca atcaaatttg
     1441 gtgcaattaa ggcatcatct tgaactttcg tttgaatact ttcgccactc atggttaaat
     1501 ctgtccaagg agaaagtaat atcaatcctt ttggaagctg taaacctaat ttcttaattt
     1561 gataagtaag tactaaagac aatccacctc cagcagaatc gccacttaca atgatttctt
     1621 gataaccatc agctaataat tgtttataaa tggcaactgc atcttctaca gcagcaggaa
     1681 aaggattttc tggtgccatt ctgtaattta tagctatagt tttatattgt gtttctctag
     1741 caattcttgc aatcaaagct cgatgtgaat gatacgaacc aacacaatat cctcctccgt
     1801 gtaaatataa aacaattcgc tttttatcta attttttagg aataaaccat gcgatatcca
     1861 aacccaaata ggtagtttct tccattttta caaatctagg aaacttaata atattagaaa
     1921 ctcgttcaaa attgattcta tgagtattgg tttctgtttt atgaataaat agaacttctc
     1981 taaacgctct gatgattcct tgggctacta aactctgtac actacgcatc aatatttttt
     2041 ttattaaatg ctaatctaac atataacatt gataaataaa aaaaattatt tacttttctt
     2101 ttgaaagatg atgagggaat ggacattttg aaataatttt ttctggtcca atctcttcca
     2161 ttttatatcc atctttataa tgatgccatt ttgccattgt acttctataa aaaggctgtc
     2221 tattaggaat taaactttcc aaaaaacttc ttgttctcaa tgcttttcta acaaaatctt
     2281 taatctgttt aggaggttca gtacgattga aagcttgaag caaatgtggc tccatcaatg
     2341 catgcaaaaa tggtcgaaaa gtttcaaaaa gactttctgg aacataccaa cttaaaaaca
     2401 tattttcagt agcatttgcc aaaagcacat tattctctgt atatttaaaa tttgttcgtt
     2461 cataatcaat atgaaactgc tctaattttt cgtaagtatt tggaatatct ttgatgccca
     2521 ttgctgcacc aatttctaac caaacataat agagagctaa ttcttcattt cgtgataatt
     2581 tgcgataagc aaattcattt atccaacgtt tactatcaaa aacaaaagtt gtaagaacat
     2641 atatataatc gtcattggaa attttaaatt gggcatgaat ccaattcatt cgctctaaag
     2701 cctcttttcc tttattgtcc tttaaaccat gttcaacaat caagctcaat aaaatatctg
     2761 tatcatcata tcgttttgca gtattatttt ccaactctct ggtttgatgt aaaattttag
     2821 atattgatgg aacagcatac gttttataca aagctaattc taatgctctt tgagcatccc
     2881 aaggaaattc ataaaacaaa gctaatcgta caatttccca atgatccttt tcaggattca
     2941 aagatttaat cttacgcaat attttatttt caaacatagt ccgctattta ttgtccaaaa
     3001 gctaataaaa tcaaaagact atataaatgt aaaacatttt attcaattca tctagaataa
     3061 atgaattata agaacattga caaaggaatt ttcaatatag aaaaatagta tttactattt
     3121 tttctttgtt gccgcttttt tagctggtgc ttttttgggt ttcttttcaa aagctccagg
     3181 cacttgtgct tctatcattt tctttaaatc ttctaatgac aagttcgcag cctcatctga
     3241 tgtatatttt ttaccggtca attttagcat ctttttatta aaacgtaaaa atggtcccca
     3301 tctcccattt tcaacagaaa ttttttcttc tggaaattct aaaatataac gattagcttc
     3361 tttatctatt ttcttaagaa ttaattcttc acattctttt tcattgatag tatcgaaatt
     3421 ataagctctc ggaatattga tatacatgtc attccattta ataaatggac caaaacgacc
     3481 ttttcctttc gttattggtt ttttatcata gtatgcaatt ggagcatctt ctacttgctt
     3541 atttttgata atttcaatag ctctttctaa gcttacatta attgggtctt ctccttttgg
     3601 taaggaaaca aattgttctc caaattttac ataaggacca aatcgtccat tatttactga
     3661 aatttcttgt tctttataaa ttcctaaagt taaaggtaac ttaaacaaat ccaatgcttc
     3721 atctaaacta atagtttcaa tactttgacc tggttttaat tttgcatacc ttggcttttc
     3781 ttcatcatca gcacttccaa tttgaaccat aggcccaaat cttcctaatc ttgctgttat
     3841 taacttatta gattctggtt ctacgcctaa ttcacgctca cctgttacaa aagtagtgct
     3901 ttctgctgaa gcttcaatat cattatggaa aggtttgtaa aactgttgga taattttact
     3961 ccattcttta ttgccttctg ctattaagtc aaaatccttt tccatatcgg cagtaaatga
     4021 ataatccata atttttggaa aattatctag caaataatct gttaccaaaa taccgatatc
     4081 cgttggtata agttttcctt tttgagcacc aacattttca gaaagtgttt cttttaaaat
     4141 attgccttta gataattcta atttttgata agcacgtggt tgtccttcta gttctccacg
     4201 aattacatat tcacgatttt gaattgtaga aattgtaggt gcataagtgg aagggcgacc
     4261 aatacctaat tcttcaagtt ttttaattaa actagcttca gtaaatcttg ctggagcttg
     4321 tgaaaatcgt tctctagctt ctattttagt ataatctaat atctgattga tattcaatgc
     4381 aggaagcata cctgcttcat cctcttcttc atcatctgta ctttcaatat atacttttaa
     4441 aaatccctca aatttcaaca cttcaccttc agcaacaaac aattcttttc tattggaaat
     4501 atcaattttg gcaattgttt tctccaaacg agcattactc atttgcgaag ctatcgttct
     4561 tttccatatc aattggtata gtttttcctc atcacgatta cttccttccg aagtattttc
     4621 aatataagtt ggcctaattg cttcgtgtgc ttcttgtgca gaagcatttt tggttgcgta
     4681 ttgttttctg ttagaataat ctttcccaaa ttctttatta atataggctt gaatagagtt
     4741 caaagctaaa tctgacaaac ttggagaatc cgttctcata taagtaatct ttccagcttc
     4801 atatagtttt tgggccacct gcatggttct tgatacagaa aaacctaact ttcttgatgc
     4861 ttcttgttgt aacgtagatg ttgtaaatgg tgcagctggt cctttttgtg ttggttttgt
     4921 ttgtatatct tttacagtaa aaattgcttg cttacaatct tctaaaaact gctctgcact
     4981 ttttatttca ctatattttt cagacaattc tgctttaaaa acttttttat cagaaataaa
     5041 ataagcaact accttataaa aagattcagt ttcaaattta ttaatttctc tttctctttc
     5101 tacaactaac ctaaccgcaa cagattgtac acgtcctgca gataaagttc ttcttcctaa
     5161 ttttttccat aaaactggtg ataattcaaa acctactact ctatctaaaa cacgtcttgc
     5221 ttgttgggca taaaataaat tagtatctaa agtacgagga ttttcaaccg cttttttaat
     5281 tgctggggct gtaatttcgt taaaaactat acgctttgtg gtatctttat ccaaattcag
     5341 tacttcgcat aaatgccaag aaatagcttc tccttcacga tcctcatctg ttgccaacca
     5401 tacttctgag ccttttgcta atttttgtaa ttctttgaca accttttctt tatcttctga
     5461 aataacataa tgtggttgaa aattattggc aatatcaatc cccatatctt tttttgccaa
     5521 atctctgata tgtccaaaac acgattttac tgtaaaatct ttacctaaga ttttttctat
     5581 cgttttagct ttcgctggcg actcaacaat tagcaagttt ttagtcataa tccaaatcca
     5641 attttattaa aaatttgtct cctatcttca aaaaagcaga agaaaaacag aaaatttggc
     5701 acaaagtaaa gcaattttaa tttgtaattc taaatttttt agaaaatata aaataaatta
     5761 caaatattta tatataaatt ttaaacttcc acagcatttt cagtcgctac ttttttcata
     5821 aatcgaccag catctgtcat tgttctttct ccagtttcaa aatttacacg gactaagcca
     5881 aatctatatt ttaaaccaat attccattcg tgattatcaa tagtactcca atggaaataa
     5941 ccttttattt taataccatc ttgttgtgct tgatgcaccc acgataaata ttcttttata
     6001 ctttcaattc taaattgcgg gtcttctgta caaacaccac tttctgtaat aataatcggt
     6061 ctgccattct gtttataata tcgatgaaaa acttcataaa atccttttgg gtcatattcc
     6121 cacatcatat cgtggcgtct tcctaattta gctaatacgc ctggattatt gatattatca
     6181 attggcaaag gtctaaatgg catttttgca taatagctca atccttgaaa atctactttt
     6241 tggaaatgct tatgacaata atccataaac caccaatcaa ataatttagc taaaagatga
     6301 cctggaaaaa cttcaccaac aaattttaca gtattatgtg aaatgccaac ttgagcattc
     6361 gggatttttt tgtgtatata atcataaact tcattatgtg ctttttccat agctttcaac
     6421 acacgtctag ctttgaacaa ttgtccttta taaaaaggtg gaaattgtcc cagtatataa
     6481 ccattactta catatacatt cggttcatta aatgtattcc aagtatctac taaatgacca
     6541 aacgtatcaa cagatttttt agcaaaatct acccacattt tataattatc acctttttcc
     6601 caactgccag tttctacaaa ccatttagga tgcgtaaaat gatgtaaaac caacatgata
     6661 tgcatacctc tatttttcaa ttcttgcata aaaaccaaat acttttcaac ttctacttta
     6721 tcaaattctg caaaaggtgc tttttgtaat ttactccaat catggctcat tctataacca
     6781 ttactcactt gacaaattaa ttctaagtct tcttctctat gagaatcgtg catactacag
     6841 ttatcaaaaa tgctaccatc aacactttta aaacccttcc aatcatgctc gcctgctttt
     6901 tcaatttgat aagctgctgt cgatgaaccc catataaaat tctcaggaaa ttgtaatttc
     6961 gtcattattg taattaattt taattaagtc gctaatatat aaatttaggt tcgtattttt
     7021 acacattagg agatgaattt aaaggactat acatataaag gtcgagtttc aattaccaat
     7081 actaaaaatt caatgcgtgt tagaattccg atgaatagaa atttggtnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnntta ccaatactaa aaattcaatg cgtgttagaa
     7261 ttccgatgaa tagaaatttg gtttatattt ctatacttat tcttactact ttcttttggt
     7321 gtctgggact tttcatgctc attgacttat ttttaaagct ccataacttc tattataaat
     7381 tatttttatt ttttggtttt ttagtttgga atggtattgg attggcaggt gtttccttct
     7441 ttatgtggat attttttgga cgtgaaagga tagttgtaaa taaagattat ttcatcacag
     7501 aaaagccttt gatatttttc tttcgccgaa acatttataa tataaatgaa gtttctaaaa
     7561 ttagaattga caaagaaaca tttaaaataa atagaaatgg gacttgggtg gaaaatcaac
     7621 gtacggtttt aaaatttgac acacccaaaa aacatgttgt atttgcaaga aatattgcag
     7681 tatcagatgc tgaatatatt ttactccttc tagccaaatg caaatactta aaagcagagc
     7741 aatttgcgat tgtgatgtaa aataataaat tatatttttg ctaaaataat aagaattggc
     7801 actttgtttg tacttttata ttcgtgagaa tattattcat tttatttttt tcgatatatc
     7861 attttataag cttcgctcaa acgcctgaca aagcattaaa atattacaat tcagctattg
     7921 aatatggaca aacggctcaa tttacagaag ctattgagga actcaaaaag gccattcaaa
     7981 tttatccaga ttacttagat gctaaaatat ttttgggaga aatttatttt agtactaaaa
     8041 aatatacgga ttgtattgcc attcttgaaa atgctaagga gaatatcaaa acaatctatc
     8101 caagtatata ccaaactaaa atgtattggc ttcttgctga tgcttattta ttagataaaa
     8161 attttgagaa atgtattatc aatgctcaaa tgtatctcaa tcaaaataaa aaaacagaat
     8221 atggaattca aaaagctaat agattgattg cgacagccaa cttttcaaaa caagccattg
     8281 ctaatccagt aaattttcat cctactaata ttggcgaaag tatcaattct gcatttgatg
     8341 attattttcc tgtactcaca ccagatgcta aacaattatt ctttactcga aaaattaatg
     8401 gacaagaaga catttactat tcggataaaa ttaataatac ttggactaaa gctcaattgc
     8461 tagacgataa agtaaataca ccgaattaca atgaaggttc gcaaagtatt tcagcaaatg
     8521 gcaatttgct attttttaca acctgcaatc gtccaaatgt ttttggtagt tgtgatattt
     8581 tttataccta tttcgatggc aaaaattgga tgcaagctaa tggaatcaat aaaccgataa
     8641 acacaagttt ttgggatgca caaccatctt acgctgcaga tggcagagct ttatatttca
     8701 gtagcgaccg acaaaatggc gttggtggaa aagatatttg ggtttcttat ttagatgatc
     8761 aattaaaatg gagtgaacca aaaaacttag gcgaccaaat caatacacct tatgatgagc
     8821 aaacgccttt tattcatcct gatggaaaaa cactttattt tatttcaaac ggacatgttg
     8881 gaatgggtat gaatgatatt ttcatgtcca aattggaaaa tggcgtttgg agcaaacccg
     8941 taaatttagg ttatcccatc aatactgaaa aagatgaaat gggcttattt gtatcaactg
     9001 atggcagtaa agcatatttt gcaagtaatc gagctgaagg ttttggaaaa ttagatattt
     9061 atgagttcga tttaccacaa gcttttaaac cacaagcgac tacttatgtg aaagtatttg
     9121 taaaaaatgc tttaaataaa aatccattat acagtcaata ttcaataatt gatataaata
     9181 caaatgaagt tataattaaa ggaaacacaa atagtgaggg taattttact gtttgcttaa
     9241 ctgccaatcg agattatgct ctaattgtac aaaaagatgg ttatttattg caaactgaga
     9301 atttttcatt taaagaaggt acaatttttc aaccttatac tttagatata tatttacaag
     9361 caattgaaaa aaacagtcag gttgtgttaa acaatatttt ttatgaatct aacagtgcca
     9421 caatcaatcc aaaatcttat acagaattag ataagttgtt tgagttcctt acaaaa
//
LOCUS       DAOQCW010000102         9407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_2767_length_9407_cov_5.704448, whole genome shotgun sequence.
ACCESSION   DAOQCW010000102 DAOQCW010000000
VERSION     DAOQCW010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9407
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2767_length_9407_cov_5.704448"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="PLS16_09105"
     CDS             <1..915
                     /locus_tag="PLS16_09105"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNJ61279.1"
                     /translation="RMQFGKQISTFGAIQHKIAEMAIRIFALEVATYRCANDIGKLEH
                     ENLSAGIDFNKALLSAAEEYAIECAMVKVLGSEVLDFIVDENVQIHGGMGFSEETSAP
                     RAYRDSRIARIYEGTSEINRLLSIDMLLKKAMSGKIDLMTPGMAIQKELMSVPDFSDD
                     SDAPFAAEEKALKNAKKAFLLVAGGAVQKLMAKLKNEQEIIMNASDMMIDIYTAESLL
                     LRVKKIYEKGEKAEQVYTDILKVFFNDAMNRIYINGKDALQSYAEGDELRIMSMGLRR
                     FTKYEPVNIKEARRRIAAKVIEANTYNL"
     gene            complement(972..1700)
                     /locus_tag="PLS16_09110"
     CDS             complement(972..1700)
                     /locus_tag="PLS16_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNJ61280.1"
                     /translation="MILIYFAAVVVVAQDKKEKLWSNKDKKAFYAKYATDTITINELK
                     IIDCVFEKIQTKFTSYKQYLHYPNSIIPIEKKCSLMVYVPITGQLSKLQAQIISNEML
                     IQLNNFRKENGNLLPLKSDADLDKAAKIQADYCAWNNELSHTQIGNNDYNTVNKRISM
                     VKKNQPKGVYLCGENIASISYEPEQLEDTLYLKEITKELLELWASSEGHRENMLYKNY
                     RFFGFAITLNDDEEALYATQVFCY"
     gene            complement(1926..2501)
                     /locus_tag="PLS16_09115"
     CDS             complement(1926..2501)
                     /locus_tag="PLS16_09115"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNJ61281.1"
                     /translation="MNETAIEILQELGIETTHSNETLMQLSVAESKYLRDLKMNVKSV
                     LTTESISPKESYLLALATATNNNNIVLINAFVNKAKSNEATDAEIAEAQACASLLAAN
                     NVFYRFRHFVDKEVYNNSPARLRMNIMMSPVLGKEFFELVSLAVSAINGCEMCVKSHE
                     QSVLANGGTEQRIWDSIRIASIITSLGKVVY"
     gene            complement(2615..3157)
                     /locus_tag="PLS16_09120"
     CDS             complement(2615..3157)
                     /locus_tag="PLS16_09120"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNJ61282.1"
                     /translation="MALLNVGQAFPSFSKQAVVSLEKGKEFATLDNSVHTKDGKWMVI
                     YFWPKDFTFVCPTEIIEFDNANGEFKDRDAVLIGGSTDSEYVHLGWRNAHEGLNKINH
                     PMLADTSKSLARELGILTDGEEVCYRATFIVDPQGIIRWVAVNDLSVGRNVKEVIRVL
                     DALQTDELCPCNWEKGQATL"
     gene            complement(3207..3686)
                     /locus_tag="PLS16_09125"
     CDS             complement(3207..3686)
                     /locus_tag="PLS16_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HNJ61283.1"
                     /translation="MGNKNKEKDKSKKNQTFANPLVDKLNLTLASYQVHYQNLRGIHW
                     NIKGKNFFELHVKFEELYTDAQVKIDEIAELILTLGYQPLHTFSDYIENSKVKPAKNI
                     SKDSEAVEMIVADLKALVQIEKEVFELAEGNISVEDLMTQFIAFQEKTLWMYKAWLG"
     gene            3775..4710
                     /locus_tag="PLS16_09130"
     CDS             3775..4710
                     /locus_tag="PLS16_09130"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HNJ61284.1"
                     /translation="MISLTQLEYVIAIDNYKHFAKAAEACNVTQPTLSMQLKKMEETL
                     NIEIFDRTKQPIVATLAGEELIQQARIILRETKQFMELAKNFNTNIVGEFKLGIIPTI
                     APYLLPYFIGSFQRNYPSVKLKIIELKTEDIIRQLNLDEIDAGILAIPLKEPNLIEEP
                     LFYEEIKVYAQKNHPLIKKKILESKDVLRTDIWMLSKGNCFRDQIINICSLHDSFEEN
                     ITYESSSLETLKKMVDIEGGFTLIPELAVLELSVKKLDNIAEFKTPKPIREVGIVYAR
                     SVAKKKILQGLKQEILNNIPEDLKDKNKGKVVEWK"
     gene            4769..5710
                     /locus_tag="PLS16_09135"
     CDS             4769..5710
                     /locus_tag="PLS16_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HNJ61285.1"
                     /translation="MSIATIDNSVSKKGFLVSAIVHTVLFLILFFIVMDNPPKQDMTD
                     AIILDFGDTEMGSGGNSDSYGGGSAPIENVAPDPQPIQTSPPPQVSTPKPTVSPKAPT
                     TSSTVTSVDANAIAIQKQQKLDAEKKKQEEIAQQKILAQQKQAAAELAKKQAEEQAAK
                     DAVASAFNKGKKGGTGGSGNGSGSGVGNNSGNGSGSGGKGNNGGSGVGDGNGGGGNGG
                     SGGGTGTNYSLSGRSLLSKKVPFNNSQKAGLVVIQIKVDKNGQVIFAQGPYKGSTTTD
                     AYLVKISEDAARNFKFSANSDAAEEQFGTITFNYTFN"
     gene            complement(5778..6476)
                     /gene="radC"
                     /locus_tag="PLS16_09140"
     CDS             complement(5778..6476)
                     /gene="radC"
                     /locus_tag="PLS16_09140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNJ61286.1"
                     /translation="MDILEQRLSIKLWAEEDRPREKLQSKGKNNLTDAELLAIIIGSG
                     NHTQTAVELAKHILSNCNNNLEELGKKSIADLLKFKGIGEAKAISIVAALELGKRRQH
                     TDAKEINKITCSKDIFKIMHPKIGDIDYEEFWLITLAKNNKIIQIKKVSHGGVSGTVV
                     DAKILFNIALQDLASGIILVHNHPSGNTNPSEQDIKITKKIRNAAENLDITLLDHLII
                     TQQSYCSFADDGIL"
     gene            complement(6534..6791)
                     /gene="rpsT"
                     /locus_tag="PLS16_09145"
     CDS             complement(6534..6791)
                     /gene="rpsT"
                     /locus_tag="PLS16_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNJ61287.1"
                     /translation="MAHHKSAQKRIRQTAKRRLENRYYGKTTRNALRKYLALTDATEA
                     QSSMSTVVSMVDKLAKRNQIHKNKASNLKSRIMLHLNGLKK"
     gene            6967..>9407
                     /locus_tag="PLS16_09150"
     CDS             6967..>9407
                     /locus_tag="PLS16_09150"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HNJ61288.1"
                     /translation="MQDLSVISNAHPSYIDAQYQDYLKDPNTVEKEWRSFFTGFDLAY
                     KYGENGAGAHLEEDKTASSKEFRVLKLIAGYRNKGHLLAHTNPLKPRKDRHAELELYN
                     FGLEESDLDIEFYSGHEIGIGKATLRTIVDRLKKIYTRTIGWEYNYMLDMEARGWMRE
                     QVEHGYIAYEHPIEKKKRILQKLNDSEVYEKFLGTKFLGQKRFSLEGGESTIPALDSM
                     LNVAANLGVKEAMIGMAHRGRLNILTNIVGKTYEEVFSEFEGVSPDDLFSGTGDVKYH
                     MGYSGIYDTMDGKEIYVKLPPNPSHLEAVNPVVQGYCRAQADAIYESDYDKILPIIIH
                     GDAAVAGQGIVYEVLQMESLKGYTVGGSIHFVINNQIGFTTDFDDARSSNYCVSIAST
                     IQAPVFHVNGDDVEAVTYVAELAAEYRQKFNKDVFIDMVCYRRWGHNEGDDPQYTQPT
                     MYHLIKDHTGVRDLYFKKLQDWGVAEKELTKNMEKEYWDELQKRLNDYKQSPKKYTPQ
                     QTDIDWEVIRKAKDEDFESSVDTKLAKTKMVKIIKAMVNTPDGFQPLKKIQQYLDHRR
                     ILMRENNSIDWAAAELMAYGAILLDGKDVRLSGEDVKRGTFSHRHACLYDEKTGEEYN
                     RLNFIDEKQGTFRIFNSHLSEYAVLGFEFGYSLSSPNPVVIWEAQFGDFCNGAQIIID
                     QFISSSETKWNRNSDLVLLLPHGYEGAGPEHSSARMERFLQLCGENNMYVCNITTPAN
                     FFHALRRQEKSPFRIPLIVMSPKSLLRHPKCISDIKEIYESKFKEIIDDENTLADSKV
                     KRVLLCSGKVYYDLL"
BASE COUNT         3235 a         1312 c         1638 g         3222 t
ORIGIN      
        1 agaatgcagt ttggaaaaca aatctcaaca tttggagcaa ttcaacataa aattgcggaa
       61 atggctatac gtatttttgc tttagaagtt gctacttaca gatgtgctaa tgatattgga
      121 aaattagaac acgaaaattt aagtgctggt attgatttta ataaagcatt attgagtgct
      181 gctgaagaat atgcaattga gtgtgcaatg gtaaaagtat tgggttctga agttttagat
      241 tttatcgttg atgagaatgt gcaaattcat ggtggtatgg gtttctctga agaaacttct
      301 gcaccaagag cgtatagaga ttcaagaatt gctagaattt atgaaggaac aagtgaaatc
      361 aatagattgt tatctattga tatgttattg aaaaaagcaa tgagtggcaa aatagatttg
      421 atgactccag gaatggcaat tcaaaaagaa ttgatgagcg ttcctgattt ttctgatgat
      481 agtgatgcac catttgcagc tgaagaaaaa gcacttaaaa atgctaaaaa agcatttttg
      541 ttggttgcag gtggagctgt tcagaaatta atggctaaat tgaaaaatga acaagaaatt
      601 attatgaatg cttctgatat gatgattgat atctatactg cggaatcatt attgttgaga
      661 gtgaagaaaa tttatgagaa aggtgaaaaa gcggagcaag tttatacgga tattctaaaa
      721 gtattcttta atgatgcgat gaatagaatt tatataaatg gcaaagatgc tttacaatct
      781 tatgcagaag gtgatgagtt gagaattatg tctatgggct tgagaagatt tacaaaatat
      841 gagccagtaa atataaaaga ggcaagaaga agaattgctg caaaagttat tgaagccaat
      901 acgtacaatt tataatttta agaaattttt tatatgaaaa cagctgccga aaggtggctg
      961 ttttttattt tttaataaca gaaaacttgc gtagcataga gtgcctcttc atcatcattt
     1021 agggtaattg caaacccaaa aaatctataa tttttataca acatattttc acgatgacct
     1081 tccgatgatg cccaaagttc taataattct ttagtaattt cttttaaata taaggtatct
     1141 tccaattgtt ctggttcgta tgatatagac gcaatatttt cgccacaaag ataaacacct
     1201 ttaggttggt ttttttttac catcgaaatt cgcttattca ctgtattgta atcattattg
     1261 cctatttgcg tatgactaag ttcattattc caagcacaat aatcagcttg aattttagcg
     1321 gctttatcta aatcagcatc agattttaag ggtaataaat tgccattttc ctttctgaaa
     1381 ttatttaatt gaataagcat ttcattagat ataatttgag cttgcaattt tgaaagttgt
     1441 ccagtaattg gaacatagac cattaaagaa catttttttt caatagggat tattgaatta
     1501 ggataatgta gatattgttt ataagaagta aattttgttt gaattttttc aaacacacaa
     1561 tcaataattt ttagttcatt tattgttatg gtatctgttg catatttagc ataaaatgct
     1621 ttcttatctt tatttgacca aagcttttct tttttatctt gagctactac aacaactgct
     1681 gcaaaataaa ttagaatcat caatagaatt tgaagattgt tttttgaaaa gatactaaac
     1741 atacagttta aacgtagaaa atttacaaat attttccata aaaggaatta aaaataagga
     1801 atagtaaaat acaatgactc gaataaaaaa agctcctaaa attctaggag cttaactttt
     1861 taagtgaata ggaattgttt tttgtttatt ctgtatatta tatacagaaa actatctaat
     1921 tttttttaat aaacaacctt acccaagcta gtaatgatag aagcaattct gatgctatcc
     1981 caaattcttt gttctgtacc accatttgcc aaaacagatt gttcgtggct ctttacacac
     2041 atttcacaac cattaatggc agaaactgct aaacttacca attcaaaaaa ctcttttccc
     2101 aatactggcg acatcataat attcatacgt aagcgggcag gagagttgtt gtaaacttct
     2161 ttatcaacaa aatgtctgaa acgatagaat acattgttgg ctgctaataa agaagcacaa
     2221 gcttgtgctt cagcaatttc agcgtctgtt gcttcattgc tttttgcttt gtttacaaaa
     2281 gcatttatta aaacgatatt gttattgttg gttgcagtcg ctaatgctaa taaataactt
     2341 tcttttggag aaatactttc agtagtaagt actgatttta cattcatttt taaatctctc
     2401 aaatacttgc tttcagctac agaaagctgc attaaagttt cattgctatg tgtcgtttca
     2461 atacctaatt cctgtagaat ttcaattgca gtttcattca taaaaaagat tttaagagat
     2521 gagatgtaag tatgtttctt acatctcatc tataaatatt tatttttttg ttgaatttaa
     2581 agtcttattt cttgacttta aatagctact aatcttataa tgttgcttga cctttttccc
     2641 agttacaagg gcaaagttca tctgtttgaa gtgcatctaa aactctgata acttctttta
     2701 catttcttcc tacagataaa tcgtttacag ctacccaacg gataatacct tgtggatcaa
     2761 caataaatgt tgctctgtaa caaacttctt caccatctgt taagatacct aattctcttg
     2821 ctaaagattt tgaagtgtct gctaacattg ggtgattgat tttattcaaa ccttcatgag
     2881 catttctcca acctaggtga acatactcag aatctgttga accaccgatt aatacagcat
     2941 ctctgtcttt aaattcgcca ttagcattgt cgaattctat gatttcagtt ggacaaacga
     3001 aagtaaaatc ttttggccaa aaataaatca ccatccattt tccatcttta gtatgcacac
     3061 tgttgtccaa tgttgcaaac tcttttcctt tttctaaact aacaacagct tgtttgctga
     3121 aacttgggaa cgcttgtccc acatttaata atgccataat ttgataaatt tatttgttat
     3181 tgaattaatt tttgtgtatt aaaattttag cctaaccatg ctttatacat ccataaagtt
     3241 ttttcttgga atgcgatgaa ttgagtcatt aaatcttcaa ctgaaatatt accttcagct
     3301 aattcaaaaa cttctttctc gatttgtacc aatgctttta aatctgcaac tatcatttct
     3361 acagcttcgc tgtcttttga aatatttttt gcaggtttta ctttactgtt ttcaatataa
     3421 tctgaaaaag tgtgtagcgg ttgatagcct aatgttaaaa taagttctgc tatttcatca
     3481 atttttactt gagcatcagt atataattcc tcaaatttta cgtgtaattc aaagaagttt
     3541 ttgcctttaa tattccaatg aatacctctc aaattctgat agtgtacttg ataagatgct
     3601 aatgttagat ttaatttatc tactaaagga tttgcaaatg tttgattctt tttgctttta
     3661 tccttttctt tatttttatt tcccataata tttctttttt attacaacac aaagataaat
     3721 tattttgttt gataataaaa atcaattaat tttatatcct aataaatttt acttatgata
     3781 agtttgactc aattagaata tgttattgct attgataatt ataagcattt tgcaaaagcc
     3841 gctgaagctt gtaatgtaac gcaaccaact ttgagcatgc agttaaaaaa gatggaagaa
     3901 actttaaata ttgaaatttt tgatagaaca aagcaaccaa ttgtcgccac tttagctggt
     3961 gaagaattaa tacaacaagc tcgtattatt ttgagagaaa ccaaacaatt tatggagctt
     4021 gctaaaaatt ttaataccaa tattgttggg gaatttaagt taggtattat cccaacgatt
     4081 gcaccctatt tgctacctta ttttatagga agttttcaaa gaaattatcc ttctgtgaag
     4141 cttaaaatta tagaattaaa aacagaagat attataagac aattgaattt agatgaaata
     4201 gatgcaggta ttttggcaat accactcaaa gagccaaatt taatagaaga acctttgttt
     4261 tatgaagaaa ttaaagttta tgcacaaaag aatcatccgc ttattaaaaa gaaaatatta
     4321 gaatctaaag atgttttaag aacagatatt tggatgctat caaaaggcaa ttgttttaga
     4381 gaccaaatta taaatatttg ttctttgcat gatagttttg aggaaaatat tacttatgaa
     4441 agttcaagtt tggaaacctt aaaaaaaatg gtagatattg aaggtggttt tactttgatt
     4501 ccagaattgg cagttttaga actgtctgtt aaaaaattag ataatattgc agaattcaaa
     4561 acgccgaaac ctatacgaga agttggaatt gtatatgcaa gaagcgtcgc taagaaaaaa
     4621 atattacaag gtttaaaaca agaaattctc aataatatac ctgaagattt aaaagataaa
     4681 aacaaaggta aagttgtaga atggaaataa ttaaaattaa cgttttctat ttttattttt
     4741 ttgtaatatt acatcaataa tttaagcaat gtcaatagca acaatagata attcggtaag
     4801 caaaaagggc tttttagtat cagcaattgt acatacagtt ttgtttttga tattgttttt
     4861 tattgtaatg gataatccac ctaaacaaga tatgacagat gcgatcattt tagattttgg
     4921 agatactgaa atgggcagtg gtggcaatag tgatagctat ggtggaggtt ctgctccgat
     4981 agaaaatgta gctccagatc cacaacctat tcaaactagt ccaccgccac aagtttcaac
     5041 tcccaaacct actgtatcgc cgaaagctcc aacaactagt tcaacagtta caagtgttga
     5101 tgcaaatgca atagcaattc agaaacaaca aaaattagat gctgaaaaga agaagcaaga
     5161 ggaaatagct caacaaaaaa tattagctca acaaaaacaa gcggcggctg aattggctaa
     5221 aaaacaagct gaagaacaag cggcaaaaga tgctgttgca agtgccttta ataaaggaaa
     5281 aaaaggagga actggaggct caggaaatgg aagtggttca ggtgttggga ataattctgg
     5341 gaatggaagt ggcagtggtg gaaaaggaaa taatggtggc tctggcgtag gcgatggaaa
     5401 tggcggcggt ggcaatggtg gttcgggcgg aggaactggt acaaattatt ctttaagtgg
     5461 aagaagttta ttaagcaaaa aagtaccttt taataattca caaaaagcag gtttggttgt
     5521 tatccaaatt aaggtagata aaaatggaca agttattttt gcccaaggtc catacaaagg
     5581 ttctacaact acagatgctt atctagtaaa aatatctgaa gatgcggctc gaaattttaa
     5641 attttctgct aactcagatg ctgccgaaga acaatttggt acaattactt ttaattatac
     5701 ttttaattag cgaatctttt ttttgaatgt aaatagaagt atcgacccaa aaatgattac
     5761 tgtttttgtg gttgtcatta caatattcca tcatcagcga agctacaata actttgttgt
     5821 gtaataataa gatgatcaag aagtgtaatg tctaaatttt cagctgcgtt tctaattttt
     5881 tttgtgattt taatgtcttg ttcacttgga ttagtgttgc cagatggatg attgtgtacc
     5941 aaaataattc ctgatgccaa gtcttgtagt gcaatattaa atagaatttt tgcatcgaca
     6001 acagtgccag aaacaccacc atggctaact ttctttattt gaataatttt attgtttttg
     6061 gctaatgtta ttaaccaaaa ttcttcataa tcaatatctc caattttggg atgcataatt
     6121 ttgaagatat ccttggaaca ggtaatttta ttaatttctt tagcgtccgt atgttgtctt
     6181 cttttaccta attctaatgc agcaacaatg ctgatagctt tagcttcacc aatcccttta
     6241 aattttaata aatctgcaat agatttttta cctaattctt ctaaattatt attgcaattg
     6301 gataaaatgt gcttggctaa ttcaacagca gtttgagtat gattacctga accaataata
     6361 atggctaata attcagcatc tgttagattg ttttttcctt tagattgtag tttttcacga
     6421 ggtcggtctt cttcggccca aagttttata cttaatcgtt gctctaatat atccataaaa
     6481 aaaagtgtgc cttataaaga cacacttttc agtttattcc ataaataaat ggattatttt
     6541 ttcaaaccat tcaagtgcaa cataattctt gattttaaat tagatgcttt gtttttgtga
     6601 atttggtttc tttttgctaa tttatctacc atagatacaa cagtactcat agaactttga
     6661 gcttcagttg catcagttaa tgctaaatat tttctaagtg cattacgagt agtttttcca
     6721 tagtaacggt tttccaatct acgtttagca gtctgtctta ttctcttctg tgctgattta
     6781 tgatgtgcca tctttattaa attgagttgc aaagatacat aaaaaattat aaatccatct
     6841 atattaaagt gaaaaaatga ttaaatccta aaaactatga ttacttagta cttaatttta
     6901 ctttttttat ctatttaatt caaacaaaag atagatattt gtgtttagaa aattatattt
     6961 tatattatgc aagatttatc agttatttct aatgcacatc caagttacat agatgcccaa
     7021 taccaagatt atttaaaaga cccaaatact gtagaaaaag agtggcgttc cttctttaca
     7081 ggatttgatt tggcttataa atatggagaa aacggtgctg gtgcacattt ggaagaagac
     7141 aagacagcat catcaaaaga atttagggta cttaaattaa tagcaggtta tcgaaataaa
     7201 ggtcatttgt tagcacatac taatccatta aaaccaagaa aagatagaca tgcagagctt
     7261 gagttgtata attttgggtt agaagaaagt gatttagata ttgagtttta ttctggacat
     7321 gaaataggca ttggaaaagc aactttaaga acgattgtag atagattgaa aaaaatctat
     7381 actcgaacta ttggttggga atacaactat atgttggata tggaagccag aggctggatg
     7441 cgtgaacaag tggaacatgg ttatatagct tatgaacatc cgattgaaaa gaaaaaaaga
     7501 atccttcaga aattgaatga ttcagaggtg tatgaaaagt ttttaggaac aaaattttta
     7561 ggtcagaaaa ggttttcttt agaaggtggt gaatctacaa taccagcatt ggattcaatg
     7621 ttgaatgtgg ctgcaaactt aggcgtaaaa gaagcaatga taggaatggc tcatagaggt
     7681 cgtttaaata tattgaccaa tatcgttggg aaaacttatg aggaagtctt ttctgaattt
     7741 gaaggcgttt ctcctgatga tttgttttca ggaactggtg atgtgaaata ccacatgggt
     7801 tattccggaa tttatgatac tatggatggt aaggaaattt atgtaaaact accaccaaat
     7861 ccatcgcatt tggaagctgt gaatccagtt gtacaaggtt attgtcgagc tcaggcagat
     7921 gctatttatg aatctgatta tgataagatt ttgccgatta ttattcatgg agatgctgca
     7981 gttgctggtc aaggcatagt ttatgaagta ttgcaaatgg aatctttaaa aggttatact
     8041 gttggcggtt ctattcattt tgtaatcaat aatcaaatag ggtttacaac agactttgat
     8101 gatgcacgtt cttcaaatta ttgtgtttca atagcatcga ccattcaggc acctgttttt
     8161 catgttaatg gtgatgatgt agaggctgta acttatgtag cggaattggc tgctgagtat
     8221 cgtcaaaaat tcaataaaga tgtttttatt gatatggttt gttatagaag atggggacat
     8281 aatgaaggtg atgatccaca atatacacaa cctacgatgt atcatttaat caaagatcat
     8341 acaggtgttc gtgatttata ttttaagaaa ttacaagatt ggggtgttgc tgaaaaggaa
     8401 cttaccaaga atatggaaaa agaatattgg gatgaattac aaaaacgatt gaatgattat
     8461 aaacaaagcc ctaaaaaata tacacctcaa caaacggata tagattggga ggtaatcaga
     8521 aaagcaaaag atgaagattt tgagagttcg gtggatacta aattagccaa aactaaaatg
     8581 gtaaaaatca ttaaagctat ggttaataca ccagatggat ttcaaccatt gaaaaagata
     8641 caacaatatt tagatcatcg tcgtatttta atgcgtgaaa ataatagtat cgattgggcg
     8701 gctgccgagt tgatggctta tggtgcaatt ttattagatg gaaaagatgt acgtttgagt
     8761 ggtgaagatg taaaaagagg cacattctcg cacagacatg cctgtttata tgatgagaaa
     8821 actggagaag agtataatag attgaacttt attgatgaaa aacaaggtac attcagaatt
     8881 tttaactcac atttatcaga atatgcggta cttggttttg agtttggata ttccttatct
     8941 tctccaaatc cagtagttat ttgggaagct cagtttggcg atttttgtaa tggagcacaa
     9001 attattattg accaatttat tagttcgtct gaaacaaaat ggaatagaaa tagtgattta
     9061 gtattgttat tacctcatgg atatgaaggt gcgggacctg aacacagctc ggcaagaatg
     9121 gaaagatttt tacaactgtg tggggagaat aatatgtatg tttgtaatat tacgacacca
     9181 gccaatttct tccatgcttt aagaagacaa gagaagtcgc cgtttagaat tccattaatc
     9241 gtgatgtctc caaaatcttt attaagacat ccaaaatgta tctctgatat caaagaaatt
     9301 tacgaatcaa agtttaagga aattatagac gatgaaaaca cattagcaga ttcaaaagtt
     9361 aagcgtgttt tattgtgttc aggtaaagta tattatgatt tgttaga
//
LOCUS       DAOQCW010000103         9394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_2775_length_9394_cov_4.756077, whole genome shotgun sequence.
ACCESSION   DAOQCW010000103 DAOQCW010000000
VERSION     DAOQCW010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9394
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2775_length_9394_cov_4.756077"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="PLS16_09155"
     CDS             <1..889
                     /locus_tag="PLS16_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNJ61289.1"
                     /translation="FATQLNFKIDEKTYHAIEPLAGRLGIISMERITTELNKIILSNQ
                     PSIGFKLLMNTKLLHQFFPEMVKLQGVSIIENYGHKDNFYHTLKVLDNICENTNNLWL
                     RWAAILHDIAKPNTQKFEKGHGWTFHGHEFVGMKMVPKIFTKLKLPLDEKMKYVQKLV
                     LLHLRPISLTKEAITDSAIRRLIFDAGDDLEDLLILCHADITSKNEEKVARYKENLLL
                     VKQRIIEVEERDKIRNWQPPIDGAIIMETFKIQPSRAVGDIKEFIKDAILDGIIPNEY
                     DAAFQLMIEKAASMQIYPK"
     gene            953..2377
                     /locus_tag="PLS16_09160"
     CDS             953..2377
                     /locus_tag="PLS16_09160"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNJ61290.1"
                     /translation="MPRFFLITIVLLFFSPKLSFAANGDSVTIRHIIIENNKRTKDFI
                     IERELTVKENQQIKANKLDSIILANRLQVFNLRIFNDVNFNITNWENDSLDLHIKIYE
                     KWTLFPLPIIKLADRNINEWWKQYKHDFSRIQYGIKFNWPNATGRNDIISFAMSFGLA
                     QMFEVGYLNPAVNKKRNFGYGTFFSYQRSRRIPINTIEDKLSYITLGKDFQYNHFQLK
                     NSFIIRSNIHATHLITGTYSYTSVSDSVLRDNPNYFINSKNFQNYFKIAYHFESDHRD
                     IKAYPTNGWLIQLGASNYGLGFMKDARLTTAYFNMSGYKQWQKHPRFSTAGFGKLSVS
                     FPRKQPYNIQDEKSLGYNQDFVRGYELYVLNGQHYLLFKTEQRFKALDFKMKNFKKLQ
                     SKPIMNKSIAYLPLNLIIKAYFDAGYVFDNQFQAKNSLKNKWIFGFGAGIDFVTFNSS
                     VFRIEYSFNRNLENRLYLHFQQAL"
     gene            2378..3253
                     /locus_tag="PLS16_09165"
     CDS             2378..3253
                     /locus_tag="PLS16_09165"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HNJ61291.1"
                     /translation="MKIGIYGRVYKEEHLVYLKQLVAQLNKQKIEVYIYSGYQKQIQN
                     SKTLLALPSFSTHIEVKDFNLLLAVGGDGTILDTLSLIRDSEIPVAGINMGRLGFLAD
                     IHKEEIEILVKAIVQSSYTIEKRTLIYVESNKNSFGEINYGLNEFTIHKIDSASMIVI
                     HVYLNGEFLNSFWADGLVIATPTGSTAYSLSCGGPIIFPTANSFVITPVAPHNLNARP
                     MIIPDDTVLSFEVESRSASYLTALDSRFHTLDNTYQIAVKKAPFTFNLIRLPESTYLK
                     TLREKLRWGEDTRNS"
     gene            3374..4486
                     /locus_tag="PLS16_09170"
     CDS             3374..4486
                     /locus_tag="PLS16_09170"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNJ61292.1"
                     /translation="MLQGVLNGIKILDLSRLLPGPLATMLMADMGAEVIKIEDPNAPD
                     YTRFMPPHINGEGVSYLALNRNKKSISLNLKSEEGRNQFYELCRTADVVIDSFRPGVL
                     AKMQLDYDTVKQINPKIIYVAVTGYGQDGPYKNMAGHDMNYLAYAGVLGINGTPENIT
                     LPGIQIADIAGGSYPTIIGCLAAILHRNRTGEGQFVDVAMTDCVLPFLSFYLTETLNS
                     KTTYKRGEHALAGSLVNYNIYKCQDNKWVALGSLEPKFWMNFCTAINKPDWVGKMMDE
                     NVKSELKALFLTQTRDTWIAQTLKADCCIAPIYELDEIEQDDYHQERNNFIEVEHEKL
                     GKIKMVNQPIKFSAIPKNQTYSAPPNMGEHNQEYLE"
     gene            4568..6403
                     /locus_tag="PLS16_09175"
     CDS             4568..6403
                     /locus_tag="PLS16_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61293.1"
                     /translation="MKIKLLITALLMAWLGTSLAQNEVLEKSYDISRKAKRGYLGGTE
                     VNDQKQTFDMIYVLPSSARKVKIETYTFNKDLDLVNTINEDWDVDKVRSRYKWFNFRG
                     DTYQTNALTASITMGGKLVFRKKLITWKYRWWYGTYIKNVKMLEKVKPKSDAGEKYVF
                     RGGAYEVDRDSSILVVAGRQDSPKDGLGSYLHYDLLKSDNEVNISVMANIDFPAPYAP
                     IFAEPLRDDNDMANDDLPRDWIVVFAPQGGVDKKANIAANKFVYFRITPEGKIVERVD
                     FDAPSSGWRILEAYEKNNSVFFYGPTISKESKYVNQLFSTGLVATTSADEEEKATENT
                     SAKSGGMFGGFKNMVNTLSGNKDMGVTQDAIDNMLDAMNFNGFALAELSNGKIKFAKE
                     TPVADFNSKAVKPDDQKDIVKFDGKKFSTFGIFFASNGDILINGQDFKLTKNKPFGAG
                     QPAGTKLYKGVYLFQFDAQGNLKHNYGIFIDQKKTAGFFNQSPLTSDMFPVSGNMIES
                     ADKSKLYWLMNICRSIHKEVDTDVNFFSTTTTSTWTPLYSVQYGTIDLNKGTTSDFKL
                     LGESEKKKFYLFPSKSLIQMNPFIIYLSETEKGDKLLLSRMDISK"
     gene            complement(6811..7200)
                     /locus_tag="PLS16_09180"
     CDS             complement(6811..7200)
                     /locus_tag="PLS16_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61294.1"
                     /translation="MFTIAQQLVPPRTLERYNSKTIVEPCIQMYLNADPIACGNLLSK
                     KTKGVITASKDNVNKIYFNITIKSNCGKNEPPMYLRFIKVKQMDIQELLQYTQDGDEI
                     YIEEFIPVNNKIDLSCSPANFYIQNPS"
     gene            complement(7363..7719)
                     /gene="secG"
                     /locus_tag="PLS16_09185"
     CDS             complement(7363..7719)
                     /gene="secG"
                     /locus_tag="PLS16_09185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNJ61295.1"
                     /translation="MFTFLAILIIILSIALVLIIIVQNPKGGGVMGSLAGSASNVIGV
                     QRAGDILEKGTWGGTIVLLFLCILTVLAAPKTAGTSDSKTKTLTEEAAKTTPTSTPTS
                     PVAPVTPGANPLNPVK"
     gene            complement(7721..>9394)
                     /locus_tag="PLS16_09190"
     CDS             complement(7721..>9394)
                     /locus_tag="PLS16_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61296.1"
                     /translation="QAETIVETPEVKSENITETLEVESPNQEPEQLSIADQILAELKH
                     RKEAESLEAKIVELPEIIETEQTETIVKTSEVKSENITETLEVETSNQELEQLSIADQ
                     ILAELKHRKEEESLVAQTVELPEIIETEQTETIEETPEVKSENINEVVDVVEQENRPI
                     SLVDQILIELKNRKETVSIQIPENESQAILELKSEAEKLEDIEKSSDEQIIESATTPT
                     SKIDTPLEAEIQSIEQPKVTAKPFSIADQILSAIQQITSKVITIPMEKAIAQIKETEP
                     SKPIEATIKSSEINVNIESIESLPIIKITNDFIFDEVQMEGFNSTQESLDNLEQSEED
                     DTYFPSIPIDELKVSPETNNDIKIEKIKSQNESNLQAIKIDSFIPKSNKTIDEKHSPD
                     IVLKEVGDFRQSSEPIKINIDEIHPIERNRHKIIEIAQKDETHTFLEWLNIVDKSYEQ
                     NTQKLDTTRKHYNYENQFILENKTELQQSILEGELDSETEIDEDVNKMAEESISFKQE
                     MATEILAKILVKQEHFEKAIEVYQKLILKYPEKSSTFVAQIEFLKNQIQ"
BASE COUNT         3260 a         1353 c         1503 g         3278 t
ORIGIN      
        1 atttgcaact caattaaatt tcaaaataga tgaaaaaacc taccatgcca tagagccttt
       61 ggcaggaagg ttaggtatca tttcaatgga acgaattaca acagaactca ataaaataat
      121 cctttctaat cagccatcaa ttggttttaa attattgatg aatacaaaat tgttacatca
      181 gttttttcct gaaatggtaa aattgcaagg cgtttcaatt attgaaaact atggacataa
      241 agataatttc taccatacct taaaagtatt ggataatata tgtgaaaata caaataatct
      301 ttggttgcgt tgggcggcaa tattgcatga tattgcaaaa ccaaacactc aaaaatttga
      361 gaaaggacac ggctggacat tccacggaca tgagtttgtt ggaatgaaga tggtgcctaa
      421 aatttttact aaattaaaat tgcctttaga tgaaaaaatg aaatatgttc aaaaattggt
      481 actattacat ctacggccta tttcattaac caaagaagca attacagact ctgccatcag
      541 aagattaatt tttgatgctg gcgatgattt agaagattta ttaattcttt gtcatgcaga
      601 tattacttca aagaatgaag aaaaagttgc acgttataaa gaaaatttat tgttagtaaa
      661 acaaagaatt atagaagttg aagagcgtga taaaataaga aattggcagc cacctattga
      721 tggtgcaata attatggaaa cgtttaaaat tcagccaagt cgtgcggttg gagatataaa
      781 agaatttatt aaagatgcaa ttttagatgg cataatacct aatgagtatg atgcagcctt
      841 tcaattaatg attgaaaaag ctgcaagtat gcaaatttat cctaaataat tgttgttatt
      901 tggtatatat taatttaatg agtaaaaaaa tttaaagtct taactttgca aagtgccaag
      961 atttttctta ataacaattg tacttttatt cttcagtcca aagctatctt ttgctgcgaa
     1021 tggtgacagt gtaacaataa ggcatattat cattgagaat aacaaacgga ctaaggattt
     1081 tatcatcgaa cgtgaattaa ccgtaaaaga aaatcaacag ataaaagcaa ataaactaga
     1141 ctctattatc ctcgctaata gattacaagt atttaatcta agaattttta atgatgtaaa
     1201 ctttaacatt accaattggg aaaatgattc cttggatttg catattaaaa tttatgaaaa
     1261 atggacactt ttccctcttc ctattataaa attagccgat agaaatataa atgaatggtg
     1321 gaaacaatac aaacacgatt tttcgaggat acaatatggt ataaaattta attggcccaa
     1381 tgcaactggc cgaaatgata ttatcagttt tgcgatgagt tttgggttag ctcaaatgtt
     1441 tgaagtgggc tacctgaatc ctgctgtcaa taaaaaaaga aattttgggt atggaacatt
     1501 cttttcttat caaagaagcc gaagaattcc tattaatacc attgaggata aactaagtta
     1561 tataacttta ggtaaagatt ttcaatacaa tcatttccaa ctaaaaaata gttttattat
     1621 tcgttccaat atccatgcaa cccatttaat aactggaact tatagttata catctgtttc
     1681 agattccgta cttcgagaca atccaaatta ttttattaat tcaaaaaact tccaaaatta
     1741 ttttaaaatt gcttaccatt ttgaaagcga ccatcgagat ataaaagctt atcctacaaa
     1801 tggttggtta atacaattag gtgcaagtaa ttatggatta ggttttatga aagatgcacg
     1861 tttaacgact gcctacttta atatgagtgg ttataaacaa tggcaaaaac atcctcgctt
     1921 ttcaactgca ggatttggaa aactttccgt atcatttcct agaaaacaac cctataatat
     1981 tcaagatgaa aaatcattag gctataatca agattttgtg cgtggctatg aattgtatgt
     2041 tttgaatgga cagcattatc tattattcaa aacggaacaa cgttttaaag ctttagattt
     2101 caagatgaaa aattttaaaa aattacaatc gaaacctatc atgaacaaat ctattgctta
     2161 tttgcctttg aatttaatta taaaagccta ttttgatgca ggttatgtat ttgacaacca
     2221 atttcaagca aaaaattctt tgaaaaataa atggattttt ggttttggtg caggtattga
     2281 ttttgtaact tttaatagtt ctgtatttag aattgagtat agttttaata gaaatttaga
     2341 aaatagatta tatttacatt tccaacaagc attataaatg aaaataggca tttacggtag
     2401 agtttataaa gaagaacatc ttgtttactt aaaacaactg gttgctcaat tgaataaaca
     2461 aaaaattgaa gtttatattt atagtgggta tcaaaagcaa attcaaaatt caaaaacact
     2521 tttagcactt ccttcattta gtacacatat tgaagttaaa gatttcaatt tattgttggc
     2581 tgtcggtggt gatggtacaa tattggatac tttgtcgtta atcagagatt cagaaattcc
     2641 agttgcaggt atcaatatgg gacgcttagg ttttttagca gatattcata aggaagaaat
     2701 cgaaatactt gtaaaagcta ttgttcaaag tagttatacc atagaaaaac gaacgctaat
     2761 ttatgttgaa agtaataaaa attcatttgg agaaatcaat tatggattaa atgaatttac
     2821 aattcataaa atagattcag cttcaatgat tgttattcat gtttacctaa atggcgaatt
     2881 tttaaattca ttttgggcag atgggttagt tattgcaaca ccaactggtt caaccgccta
     2941 ttctttgagt tgtggtggtc caattatttt tccaactgca aattcatttg taatcacacc
     3001 agttgcacct cataatttga atgcacgtcc aatgataatt cctgatgata ccgttttatc
     3061 ttttgaggta gaaagtcgtt ctgctagtta tttaacagca ttagattcta ggtttcatac
     3121 tttagataat acttatcaaa tagccgtaaa aaaagcacca tttacattta accttattcg
     3181 tttgcctgaa agtacttacc ttaaaacatt aagagagaaa ttgcgttggg gagaagatac
     3241 ccgaaattct taattcacta tattataatt ttttttatgt ttttgtcaaa ataaactttg
     3301 gataagtatt tcatttgaat ttattaaaaa ccaaaaatta ctttatatat tgttagataa
     3361 aatattaagc aatatgttac aaggtgtatt aaacggaatt aaaattttag acttatcaag
     3421 attattacct ggtccattag caactatgtt gatggcagat atgggtgcgg aagtaataaa
     3481 aattgaagac cccaatgctc ccgactatac ccgatttatg ccgcctcata ttaatggtga
     3541 aggtgtaagc tatttggcat tgaatagaaa taaaaaatca atttcactaa acctcaaatc
     3601 agaagaaggt cgtaatcagt tttatgaatt atgcagaact gccgatgttg ttattgattc
     3661 ttttcgtcca ggtgttttgg ctaaaatgca attagactat gacaccgtaa aacaaatcaa
     3721 tccaaaaatt atttatgttg ctgtaactgg ctatggacaa gatggtccgt ataaaaatat
     3781 ggctggacat gatatgaatt atttagctta tgctggtgtt ttgggtatta atggcacacc
     3841 tgaaaatata acattaccag gtattcaaat tgcagatatt gcgggcggtt cgtatccgac
     3901 tattattggc tgtttggctg caattttgca cagaaataga actggcgaag gtcagtttgt
     3961 agatgttgct atgacagatt gtgttttgcc atttttatca ttttatttaa ctgaaacatt
     4021 gaatagcaaa actacttata agcgagggga acacgcattg gcaggtagtt tagtgaatta
     4081 taacatctat aaatgtcaag ataataaatg ggtggcatta ggcagcttag aacctaaatt
     4141 ctggatgaat ttctgtactg ctatcaataa acctgattgg gtaggcaaaa tgatggatga
     4201 aaatgtaaag tctgaattga aagccttgtt cctcacgcaa actagagata cttggattgc
     4261 acaaacctta aaagcggatt gttgcattgc tccaatatat gaattagacg agattgagca
     4321 agatgattat catcaagagc gaaataattt tattgaagtc gaacacgaaa aattagggaa
     4381 aataaaaatg gtcaatcagc cgattaaatt ctcagctatt ccaaaaaatc aaacctattc
     4441 agctccacca aatatgggtg agcataatca agaatattta gaataatttt agattattat
     4501 ttttaacatt cagactattt acattctcat aaattaaatg catatttgta taaataaaaa
     4561 aaatactatg aaaattaaat tattgattac tgctttactt atggcttggt tgggtacaag
     4621 tttagcacaa aatgaagttt tagaaaaatc gtacgacatt tctcgaaaag ccaagcgtgg
     4681 ctatttagga ggcactgaag taaatgatca aaagcaaact tttgatatga tttatgtact
     4741 gccttcatct gctagaaaag taaaaattga aacttatact ttcaacaaag acttagattt
     4801 agtgaacaca ataaatgaag attgggatgt agataaagtt agaagtaggt ataaatggtt
     4861 taattttaga ggagacacct atcaaaccaa tgcattaaca gcatcgataa ctatgggtgg
     4921 aaaattagtg ttccgtaaaa aattaattac ttggaaatat agatggtggt atggtactta
     4981 catcaaaaat gtaaaaatgt tggaaaaagt aaaaccaaaa agtgatgctg gcgaaaagta
     5041 tgttttcaga ggtggtgctt atgaagtgga tcgagatagt tctattctag ttgttgcagg
     5101 aagacaagac tcaccaaaag atggattagg ctcttactta cattatgatt tattgaaaag
     5161 tgataatgaa gttaatatta gcgtaatggc aaatatagat ttcccagctc cttatgcacc
     5221 aatctttgct gagcctctca gagatgataa tgatatggct aacgatgatt tgccaagaga
     5281 ttggattgtc gtttttgcac ctcaaggtgg agtagataaa aaggcaaata tcgctgcaaa
     5341 taaatttgta tattttagaa ttacacctga aggaaaaata gtagaaagag tagatttcga
     5401 tgctccttct agtggttgga gaatattaga agcttatgag aaaaacaatt cagtattctt
     5461 ctatggccca acgatttcaa aagaatcaaa atatgttaat caattattta gcacaggatt
     5521 agtagccact acttctgccg atgaagaaga aaaagcaact gaaaatacaa gtgcaaaatc
     5581 tggaggaatg tttggtggtt ttaaaaatat ggtcaatact ttatcaggta ataaagatat
     5641 gggcgtaact caagatgcta ttgataatat gttagatgcg atgaacttta atggatttgc
     5701 tttagctgag ctatcaaatg gaaaaataaa atttgctaaa gaaacgcctg ttgctgattt
     5761 taattcaaaa gcagttaagc cagatgatca aaaagacatt gtcaaatttg atggtaaaaa
     5821 attctctact tttggtattt tctttgcatc taatggcgat attttaatca atggacaaga
     5881 ctttaaattg actaaaaaca aaccatttgg agctggacaa ccagctggaa caaaattata
     5941 taaaggtgta tatttgttcc aatttgatgc tcaagggaat ttgaaacaca attatggtat
     6001 ttttatagac caaaagaaaa cggcaggttt ctttaatcaa tctccattga catcagatat
     6061 gttcccagtg tctggaaata tgatagaatc tgctgataag tctaaattgt attggttaat
     6121 gaatatttgc agatcgatac ataaagaagt ggatacagat gttaatttct tctctacaac
     6181 aacaacttca acttggacac cattatatag tgttcaatat ggaacaattg acttgaacaa
     6241 aggaactact tcagatttta aattattagg ggaatctgaa aagaagaaat tctatttatt
     6301 cccatcaaaa agtttaattc aaatgaaccc tttcatcatt tatctatctg aaactgaaaa
     6361 aggcgataaa ttattattat cgagaatgga tatttcaaaa tagaaattca ttaattctaa
     6421 aaataaaact gccattcttt ttaagaatgg cagttttgtt tttagtaaag agaaaaaagc
     6481 aaaatattat aaacaattct actaaaaatc agatcggaac ttaggataac aatatcacaa
     6541 aatcctttta atgctatatt tttaagccaa acaaaaaaat aagcattcat acttattcac
     6601 aattcttcat aaataacaac ttgacaaaga ttggtatttt gattaatatt gaagtataaa
     6661 ataaagctat tatgaaatta gcacacgacc aatatgttga attactcaaa tacactgcgt
     6721 atttaagtgt aatttttttc ttagcagtac tttgtaatta aatgtattga tttaaagaat
     6781 accttaatta attaatttct tcaaattagt ttaagaaggg ttttggatat agaagtttgc
     6841 aggagagcaa cttaaatcta ttttattatt tacaggaata aattcctcaa tataaatctc
     6901 atcgccatct tgagtatatt gcaataattc ttgaatatcc atttgtttta cttttataaa
     6961 acgcaaatac attggcggct cgtttttacc acaatttgat tttatcgtaa tattaaagta
     7021 tattttattg acattatctt ttgaagcagt aattacgcct tttgttttct tagataacaa
     7081 attaccacac gcaattgggt ctgcatttaa gtacatttga atacaaggtt caactatagt
     7141 tttggaatta tatctttcta aagtgcgagg tggtactaat tgttgtgcta tggtaaacat
     7201 aaccgaacaa actaaaaata ttaatatgta ccatttccct ctcatagaat gaaaatctaa
     7261 attttatcta ataacggaca aatattgtac caatttgtca attaatgtta aaattagaca
     7321 aattatcgaa gaaatgcaaa aaaaattaac ttagaactaa tattatttaa ctgggttcaa
     7381 tggattagct ccaggagtta ctggtgctac tggagatgtt ggcgtacttg taggtgtcgt
     7441 tttggcagct tcttcagtca aagttttagt ttttgaatca gaagtacctg cagtttttgg
     7501 tgcagcaagt acggttaaaa tacataaaaa caataaaacg atagttccgc cccaagttcc
     7561 tttctctaaa atatctccag ctctttgcac gccaattaca ttagatgcag aacctgcaag
     7621 acttcccatc acaccgccac cttttggatt ttgtacaata ataattaaaa caagagcaat
     7681 gctcaaaata attataagta tagctagaaa agtaaacata ttattgtatt tgatttttta
     7741 aaaattcaat ttgggcaaca aaggtactac ttttttcagg atatttcaaa attaattttt
     7801 ggtacacctc aatcgctttc tcaaaatgtt cttgtttgac taaaattttt gccaatattt
     7861 cagtagccat ttcttgttta aaagaaatac tttcttccgc cattttgttt acatcttcat
     7921 caatttcagt ttcgctatcc aattcgcctt ctaaaatact ttgttgtaat tctgtcttat
     7981 tttctaaaat aaactgattt tcataattat aatgctttct cgttgtatct agcttttgtg
     8041 tattttgttc gtaactttta tccactatat tcagccattc taaaaatgta tgcgtttcat
     8101 ctttctgtgc aatttcaata attttatgac gatttctttc tatcggatgg atttcatcaa
     8161 tattgatttt gataggttcg ctactttgtc taaaatctcc aacctctttt aaaacaatat
     8221 cagggctatg tttttcgtca atagttttat tggattttgg aatgaatgaa tctattttaa
     8281 ttgcttgcaa attactttca ttttgagatt taatcttttc tattttaata tcattattgg
     8341 tttctgggct tactttcaat tcgtctattg gaatagaagg aaaataagta tcatcttctt
     8401 ctgattgctc caaattgtct aagctttctt gagttgaatt aaaaccttcc atctgaactt
     8461 catcaaaaat aaaatcattt gtaattttta ttattggcaa tgactcaatg gattcaatat
     8521 ttacgtttat ttctgacgat tttatagttg cttcaattgg tttagatggc tccgtttctt
     8581 taatttgagc aatcgctttt tccattggaa tggtaattac cttactagta atttgttgta
     8641 tggcagaaag tatttgatct gcaatagaaa aaggcttcgc tgttacttta ggttgctcaa
     8701 tactttgaat ttctgcttct aatggtgtgt caatttttga agttggagtt gttgctgatt
     8761 cgataatttg ttcatctgaa gatttctcta tatcttccaa cttttcagcc tctgatttta
     8821 attccaatat agcctgactt tcattctcag gtatttgaat actaaccgtt tcttttctat
     8881 tttttaattc aattaaaatt tgatctacta aagagattgg tctattttct tgctcaacaa
     8941 catcaactac ttcattaata ttttccgatt taacctctgg agtttcttca atagtttcag
     9001 tttgttctgt ttctataatt tcaggtaatt ctaccgtttg tgctactaaa ctttcttctt
     9061 cttttctatg ttttaattca gccaaaatct gatctgcaat agatagttgc tccagttctt
     9121 gatttgacgt ttcaacttca agtgtttcag taatattttc agatttaact tctgaagttt
     9181 ttacaatagt ttcagtttgt tcagtttcta taatttcagg caattcaaca attttcgctt
     9241 ctaaactttc tgcttctttt ctatgtttta attcagccaa aatctgatct gcaatagata
     9301 attgctctgg ttcttgattt ggactttcaa cttcaagtgt ttcagtaata ttttccgatt
     9361 taacttctgg agtttctaca atggtttcag cttg
//
LOCUS       DAOQCW010000104         9190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_2865_length_9190_cov_5.081007, whole genome shotgun sequence.
ACCESSION   DAOQCW010000104 DAOQCW010000000
VERSION     DAOQCW010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9190
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2865_length_9190_cov_5.081007"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1748
                     /locus_tag="PLS16_09195"
     CDS             <1..1748
                     /locus_tag="PLS16_09195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HNJ61297.1"
                     /translation="VWTGSKWENVGNIQGPKGDKGEKGDVGANGQQGIQGIKGDKGDK
                     GDVGATGPQGIQGIQGLKGDNGAIGSQGIQGVKGDKGDIGPQGPQGIKGDKGDKGDVG
                     ATGPKGDKGDSFANGTAINQMLVWNGTAWVAVAPPTTASQVLTFNNGKIGWETSMNML
                     PLVRTFECSTFYREDYFVFVLKYEIIHFGGSDVTEYGFSRFYANGSYMTKRSHTDNIK
                     VGVFFDTIYTSLHDASVIVGQEVYLYYKAFATNSYGTGYGNMIKARVELALAEFNPLS
                     IIDITSNSVNVKASINTTGGMWKGEKGFCWSTSPNPTINNDFKKNTDTFLIAPFTDVI
                     TGLEANTTYYIRAYATNLMGTSYSNELSFKTNPLDITNKPYLNHELSYGSISYNGYIY
                     PTIKIGNQTWMAENLRTEHYNDGTVIPYLRDSAAWVNTVNGAYSFYNNRPNLEALYGK
                     LYNWYAVNTGKLCPKGWHIPTKEEWTSLITYLSGDDNPGGKMKSTGNKTDGTGLWSSP
                     NTGATNESGFSGLPGGARYYGDYAYIGNSGFWWSSTVYSTSVFYWPLIYDDNFYEPQA
                     IEKKVTGMSCRCLKD"
     gene            2235..3599
                     /locus_tag="PLS16_09200"
     CDS             2235..3599
                     /locus_tag="PLS16_09200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNJ61298.1"
                     /translation="MRKVVFIDGVRTPFLKSGTNYMDLMSYQLGQLAIKGLLDKTGIE
                     PSEIDRVVYGNVISNVKTENVARESAITAGVPVRVPANTVKQACISANQAISNAMDFI
                     QLGRADVVISGGTECTSDTPIGYSKEMRKKLFMAQKLSTPMEYAKFATTLRPNDFLPD
                     RPAVQEFITGRTMGQDCELMVQKFGVTREEQDELAAASHQNAGKAQEAGLLDDIISVQ
                     LPPNFEPITKDNGVRGDTTVDKISKLKPAFDKYVGTITAANASFLTDGASAVLLTSED
                     KAKELGLTPKAELVDYTFTAGDLYDELLLGPAYSIAEVLKKNNLTIDDIDVWEIHEAF
                     AGQVCSVLKALENEEWCKAHVGYDKAVGSIPKNKINQWGGSLSIGHPFGATGGRLLTM
                     AANRLVKGGGKYAILTACAAGAHGTAMLIKRYESTEQKVKSAAKSVISKAEDALEDIK
                     EKLG"
     gene            3712..5892
                     /locus_tag="PLS16_09205"
     CDS             3712..5892
                     /locus_tag="PLS16_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNJ61299.1"
                     /translation="MKREDFLSVERNPNGVCTIWMDQKNEKINKVGPDLIQLFNEIFE
                     ELFEDNSIKAFVINSKKKDFIAGADIEMFQKVKQKGDFEPITRRGHQILNRVQNSKKP
                     IVAAINGNCLGAGLEIALACHARVASNDKSTKMALPEVQLGLLPGGGGTQRLPRLVGL
                     QAALDMMLTAKNIFPYPALKMGLVDKVVHLNTLQMTAEKLALELIDKPIVRDRNAINL
                     DTVKSGFPALQSKLTNLVLEAPLVNKIVFDQARKMVNKQTHGCYPAPFKIIECVEIGW
                     NEGLEAGFDAEAKKFEELILSDVSRQLINIFFAMTNKKKNPYDIKLVREVDRIGMVGA
                     GFMGAGIAEVSMNGGMHVLMKDINQDMLNSGFKGIYNSYNKKVKKKAMTSIELEEKMT
                     LLSGSLDYRDLHNQDIIVEAVFEDLKLKQNILADCEANAKKDTIFATNTSALPIKDVA
                     AKAKRPELVIGMHYFSPVPKMPLLEIIKTDKTADWVIATCYEVGIRQGKTVIVVNDGP
                     GFYTTRILAPLMNESQLLLDEGGDILQIDKEMNLFGFPVGPITLADEVGIDVGAHIMG
                     GELMTELLSSRPKFQVSMTLLEVSKAGYKGRKNKKGFYQYDENGKKQPKVDSEIYKFY
                     GGNNRKQFDAEMIHLRCAMAMVNEAALCLQEGIISSPLDGDIGAVFGLGFPPFRGGPF
                     RYIDSLGAAKTVDILNDLASKYGERFDPAQILIDYAKAGKKFYA"
     gene            5999..6766
                     /locus_tag="PLS16_09210"
     CDS             5999..6766
                     /locus_tag="PLS16_09210"
                     /inference="COORDINATES: protein motif:HMM:NF026121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HNJ61300.1"
                     /translation="MKRIFTLFFLAFSFVQFAKADAEFFLKIKQQGNYKVWLNQEMYE
                     TKKNNIVRFFDLIPGNYNLKVVRQMGWMQQIIFQENIQLQNGFRYAAELQPQYGLKML
                     AQIPFVQNEWYIDQLITNNYPNQYPPNYYPNNYPNQPHQQPDWNHQHNSNNNGWNNYY
                     GMDDNSFKQLRNTVSKSVFDVDKIEVAKSALAKNYITSAQAKELLSLINFESNRLTLA
                     KFCYDKTLDQQNYFTVYDTFTFSSSVNELSDYISKQR"
     gene            complement(6767..7501)
                     /gene="rlmB"
                     /locus_tag="PLS16_09215"
     CDS             complement(6767..7501)
                     /gene="rlmB"
                     /locus_tag="PLS16_09215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNJ61301.1"
                     /translation="MQAQYIIGRNSILEAFEEETNFEKIYISKQAHGDAVKKIIQKAK
                     EKDVYVQYVPEIKLDYLANKQPHQGVVAAIQLIAYYNFQEIVDFCYNEGKTPLFIILD
                     GITDVRNFGAIARTALGLNADLIIIPKRESVSITADAIKTSAGALLKIPVAKVDNLHT
                     TIKDLKNNGIKIIGLEGTATQTFQEADLKVPMALVLGAEDKGISTPVLKLLDEKLKLP
                     MNEELESYNVSVANALALYEIYRQRN"
     gene            complement(7566..>9190)
                     /locus_tag="PLS16_09220"
     CDS             complement(7566..>9190)
                     /locus_tag="PLS16_09220"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HNJ61302.1"
                     /translation="YYLDRKWTYQEFDEWCNRLANHFVAIGIKKGDVVAVLLENRPEL
                     LAVSMALSKIGGIAALLNTAQRNKPLIHSIQLAQPKLLLIDQNLINNYNEIKNTLNFP
                     NNLVFSIADELKRTSSQLKIFDIKSFQYSNQKPNYQPKIFAKDPAMYIYTSGTTGLPK
                     ASIISHGRWLKGYSAFGLTSLRLNEKDILYVPLPFFHATAMVVCWSSVVAGGAAIVIK
                     NKFSVSDFWKDIHKYQVTGFGYVGEMCKYLLNTPENPLEKNSTLKKMIGNGLRPDIWQ
                     KFKQRFNIEQVAEFYASSEGNIAFFNVFNLDQTMGFSITSYAIVKYDIEKGAPKLDKN
                     GFMQKVKTNGVGLLIGEINERYPYDGYTEKSKTEQTILRNVFTKGDAWFNTNDLVRDM
                     GFGHTQFVDRLGDTFRWKGENVSTQEVEGIINLIDNVEESIVFGVEIPGCNGRAGMAK
                     IILKENKALDMDNLYRLLSNELPAYAVPIFVRIARDAAITSTFKHQKSELKKEGFDIN
                     IIKDDVYVLKNKQYILLDKDFHEEICKGDIRF"
BASE COUNT         3177 a         1325 c         1673 g         3015 t
ORIGIN      
        1 atgtatggac aggaagtaaa tgggaaaatg taggcaatat tcaaggtccc aaaggagata
       61 aaggcgaaaa aggagatgtt ggtgcaaatg gtcagcaagg catacaaggt ataaaaggcg
      121 acaagggaga taaaggggat gttggtgcaa ctggaccaca aggtattcag ggtattcaag
      181 gcttaaaagg ggataatggt gcaattggtt cacaaggtat tcaaggcgta aaaggcgata
      241 agggtgatat tggaccacaa ggaccacaag gtataaaagg agataaaggc gacaagggag
      301 atgttggagc aacaggacct aaaggcgaca aaggagattc gtttgccaat ggtactgcta
      361 ttaaccaaat gttggtttgg aatggtactg cttgggtagc tgttgcacct ccgactacag
      421 catcacaagt gcttactttt aacaatggaa aaataggatg ggagacctcc atgaatatgt
      481 taccattagt aaggacattt gaatgtagta ccttttatag agaagattat tttgtatttg
      541 ttttaaaata tgaaataatt catttcggtg gaagtgatgt aacagaatat ggtttttcta
      601 ggttttatgc aaatggtagt tatatgacta agagaagtca tacagataat ataaaggtag
      661 gagttttttt tgatactata tatacgtcgt tacatgatgc aagtgtaatt gtaggtcagg
      721 aagtctattt atattataaa gcttttgcta ccaatagtta tgggacaggt tatggaaata
      781 tgattaaagc tagagtagaa cttgcgttag cagaattcaa tccactttct attatagata
      841 tcacatcaaa ttctgttaat gttaaagctt caattaatac tacgggagga atgtggaaag
      901 gagaaaaggg attttgttgg agtactagcc ccaatcctac tataaataat gactttaaaa
      961 agaatactga tacttttttg attgcaccat ttactgatgt aattactgga ttggaggcta
     1021 atacaacata ttatataagg gcttatgcaa ctaatcttat gggaacatcg tatagtaatg
     1081 aactaagttt taaaacgaac ccattagata ttacgaataa gccttacctc aatcatgaat
     1141 taagctatgg tagtattagt tacaatggtt atatctaccc tacaataaaa ataggaaacc
     1201 aaacatggat ggcagagaat ttaagaactg aacattataa tgatggaaca gtaattcctt
     1261 atttaagaga ttcagcagca tgggttaata ctgtaaatgg agcctattca ttttacaata
     1321 acagacctaa ccttgaagct ttatatggaa aactgtataa ctggtatgcc gtaaatacag
     1381 gcaagttatg ccctaaaggt tggcatattc ctacaaagga agaatggact tctctgatta
     1441 cctatttaag tggtgatgat aatcctggtg gaaaaatgaa atctactgga aataagactg
     1501 atggtacagg tttatggtca tcgcctaata cgggtgctac caacgaaagt ggcttttctg
     1561 gtcttccagg tggtgcacgt tactacggtg attacgctta cattggcaac agcggtttct
     1621 ggtggagttc tacagtgtac agtacttctg ttttctattg gcctttgatt tatgatgata
     1681 atttttacga accgcaggcc atagaaaaaa aagttacagg tatgagttgc cgttgtttga
     1741 aagattaaaa taaaaatata ataagaccgt tgcaaaagtc aaattacaaa aatatccaag
     1801 gctttcattt atttttacac cgcagttatc gtattgataa tgaggattaa aaataaatga
     1861 gtaacgaaga agattgaagg aatttgactt gatttttata aataatgatt gccttgcaac
     1921 ggtcttataa tatcaaaaaa aaactctgta ttgtgtaaga tacagagggc ttttttatag
     1981 aatctaattt ttaaataaga cactagtata atattttaaa gacaatttaa ggaattgtga
     2041 ttgtgtgaaa tcataattcc tttttatttt tgaatacgat acaataatta gaaataacgc
     2101 tgataaatac tcattttttc taccgttcaa ttatccaaag ataaatctgt aaggtataac
     2161 ttaaaattaa cttcaaattt acaatgcatg cattgtaaat ttgtatattt ggttaataaa
     2221 aaataaatta tatcatgaga aaagtagttt ttatagacgg tgttcgtaca ccatttttaa
     2281 agtcgggaac aaattatatg gatttaatgt cttatcaatt aggacaatta gcaattaaag
     2341 gattgttaga caaaacgggt atcgagccaa gtgaaattga tagagtagtt tatggaaatg
     2401 taatttcaaa cgttaaaaca gaaaacgtag ctcgtgagtc agcaattact gctggtgtgc
     2461 cagttagagt acctgcaaat acagtaaaac aggcttgtat ttctgcaaat caagccattt
     2521 caaatgcaat ggattttatc caattaggca gagcagatgt tgtgatttct ggtggtacag
     2581 aatgtacctc agatacacca attggttact ctaaagaaat gcgtaagaaa ttatttatgg
     2641 ctcaaaaact ttctacgcca atggagtatg caaaatttgc aacaacatta cgtccaaatg
     2701 attttttacc agatagacca gcagttcaag aatttattac aggaagaact atgggacaag
     2761 attgtgaatt aatggtacaa aaatttggag taaccagaga agaacaagat gaattagcgg
     2821 ctgcttctca ccaaaatgcg ggtaaagcac aagaagctgg tttgttagat gatattattt
     2881 cagtacaatt gccaccaaat tttgaaccaa tcacaaagga taatggcgtt cgtggagata
     2941 caacagttga taaaatctct aaattaaaac cagcattcga taaatatgta ggaactatta
     3001 ctgcggcaaa tgcatctttc ttaacagatg gagcatcagc tgtattatta acttctgaag
     3061 ataaagcaaa agaattaggt ttaacaccaa aagcagagtt agtagattat acttttactg
     3121 ctggagattt atatgacgaa ttgttattag gtcctgctta ttctattgct gaagtattga
     3181 agaaaaacaa tctaacgatt gatgatattg atgtttggga gatacacgaa gcatttgctg
     3241 gtcaggtttg ctcagtatta aaagctttag aaaatgaaga gtggtgtaaa gctcatgttg
     3301 gatacgataa agcagtaggt tctattccaa aaaataaaat caatcaatgg ggtggctcac
     3361 tttcaattgg acatccgttc ggtgctactg gtggtcgttt attaacgatg gctgctaata
     3421 gattagtaaa aggtggcggt aaatatgcta tcctaacggc ttgtgcagct ggagctcatg
     3481 gaactgctat gttgattaaa agatatgaat ctactgagca aaaagtgaaa tctgctgcaa
     3541 aaagtgtaat cagcaaagct gaagatgctt tagaagatat taaagaaaaa ttaggataat
     3601 cttagataat aggcacaagt agtaagacaa gagtaggaat tactttagtt gaataacttg
     3661 tgcttttttc tttaagaaaa ttactcctat caaattatta aataaaaaaa tatgaaaaga
     3721 gaagattttt taagcgttga gcgaaatcca aatggggttt gtaccatttg gatggatcaa
     3781 aaaaacgaaa aaataaataa agtaggacct gatttaattc agctttttaa tgaaattttt
     3841 gaagaactat ttgaagataa cagtatcaaa gcatttgtaa tcaatagtaa gaaaaaagat
     3901 tttattgcag gagctgatat agaaatgttc caaaaagtaa aacaaaaagg cgattttgag
     3961 ccaattacac gtcgtggtca tcaaatttta aatagagttc aaaattctaa aaagccaatt
     4021 gtagcagcta tcaacggaaa ttgcttaggt gctggtttag aaattgcatt agcttgtcac
     4081 gctcgtgttg catctaacga taaatctaca aaaatggctt tgccagaggt gcaattaggt
     4141 ttgttgccag gtggtggtgg aactcaaaga ttaccacgct tagtaggttt acaagcagct
     4201 ttagatatga tgctgactgc taaaaacatt ttcccttatc ctgctttgaa aatgggattg
     4261 gtggacaaag tggtgcatct aaatacgcta cagatgactg cagagaaatt agctttggaa
     4321 ttaatagata agccaattgt aagagataga aatgcaatta atttagatac ggtaaaatca
     4381 ggctttccag cattgcaatc caagttaaca aacctagttt tagaagcacc tttagtaaat
     4441 aaaattgtgt ttgaccaagc aagaaaaatg gttaataaac agacgcatgg ttgttatcct
     4501 gcacctttta agattattga atgtgttgag attggttgga atgaaggttt agaagctggt
     4561 tttgatgcag aagctaagaa atttgaggaa ttaattcttt cagatgtttc tagacaatta
     4621 atcaatatct tttttgctat gaccaataag aagaaaaatc cttatgatat aaaattagtt
     4681 agagaagtag atcgcatagg aatggtgggt gctggtttta tgggtgctgg tattgctgaa
     4741 gtttctatga atggtggtat gcatgttttg atgaaagata ttaatcaaga tatgctaaat
     4801 tctggattta aaggaattta taattcttat aacaaaaaag ttaagaaaaa agcaatgact
     4861 tctattgaat tggaagaaaa gatgactttg ctttctggct ctttagatta tagagattta
     4921 cacaaccaag atattatcgt agaagctgtt tttgaagatt tgaaattgaa acaaaatatc
     4981 ttagcagatt gtgaagcaaa tgcaaaaaaa gatacaattt ttgcaaccaa tacttctgct
     5041 ttgccaatta aagatgtggc tgccaaagca aaacgtcctg agttggtgat tggtatgcac
     5101 tatttttctc cagttcctaa aatgccttta ttagaaatta ttaagacaga taaaacggct
     5161 gattgggtta ttgcaacttg ttatgaagtg ggcatccgtc aaggtaaaac agttattgtg
     5221 gtaaatgatg gtccaggctt ttatacaact agaattttag caccattaat gaatgaatct
     5281 cagttattat tagatgaagg tggtgatatc ttacaaattg ataaagaaat gaatttgttt
     5341 ggttttcctg taggtccaat tactttagct gatgaagttg gtattgatgt tggtgctcat
     5401 attatgggtg gcgaattaat gactgaatta ctttcttctc gtcctaaatt tcaagttagt
     5461 atgactttgt tagaagtttc taaagcaggt tacaaaggta gaaaaaacaa aaaagggttc
     5521 tatcaatatg atgaaaatgg taagaaacaa cctaaagttg atagtgaaat ttataaattc
     5581 tacggtggca ataatagaaa gcaatttgat gctgaaatga tacatttacg ttgtgcaatg
     5641 gcaatggtta atgaagcagc tttgtgtttg caagaaggta taatttctag tccactagat
     5701 ggcgatattg gagctgtatt tgggttaggt tttcctccat ttagaggagg tcctttccgt
     5761 tatatagatt cattaggtgc tgctaaaaca gtagatattt taaatgattt agcatctaaa
     5821 tatggcgagc gttttgatcc agctcaaatc ttaattgatt atgcaaaagc aggtaagaaa
     5881 ttttacgctt aaattagaat aactgtatag ttaaaaaagg tcaagcattt gcttggcctt
     5941 tttttatact tgatttggca tagattttga attttgttga ttaagaaatt caaatattat
     6001 gaaaagaatc tttacactat tttttttagc tttttctttt gttcaatttg caaaagcaga
     6061 tgctgagttt ttcttgaaaa taaagcaaca aggaaattat aaagtttggc ttaatcaaga
     6121 aatgtacgaa acgaaaaaga acaatatcgt tcgctttttt gatttaatac cagggaatta
     6181 taatttaaaa gtggttcgac aaatgggttg gatgcaacag attatttttc aggaaaatat
     6241 tcaactacaa aatggtttta gatatgcagc agaattacaa ccacaatatg gtttaaaaat
     6301 gcttgcacaa attccttttg tccaaaatga atggtatatc gaccagttga ttacaaataa
     6361 ttatccaaat caatatccgc caaattacta tcctaataat tatccaaacc aaccacatca
     6421 acagccagat tggaatcatc aacataattc aaataataat ggatggaata attattatgg
     6481 aatggatgat aatagtttta aacaacttag aaatacagta agtaaatccg tttttgatgt
     6541 agataaaata gaggttgcga aatctgcttt agctaaaaat tatattactt cagcacaagc
     6601 aaaagaatta ttatccttaa ttaattttga gtcaaacaga ttgacattag caaaattctg
     6661 ttatgataaa acacttgacc aacagaacta ttttacagtt tatgatactt ttacattctc
     6721 tagtagtgtg aacgaactaa gtgattatat ttctaaacaa agatgattaa ttcctttgtc
     6781 gatagatttc gtacaaagcc aaagcattag ccactgaaac attgtaagat tctaattctt
     6841 cattcattgg tagcttaagt ttttcatcta ataatttaag aactggtgta gaaattcctt
     6901 tgtcttcagc acctaaaact aaagccattg gaacttttaa gtctgcctcc tgaaatgttt
     6961 gagttgcagt gccttctaat ccaataatct taatgccatt attttttaaa tccttgattg
     7021 ttgtatgtaa attatcaact ttagcaactg gaatttttaa taatgcacct gctgaagtct
     7081 ttatggcatc agcagtaatg ctaacacttt ctcttttagg aatgataatt aaatcggcat
     7141 ttaaacctaa agccgttctt gcaattgctc caaaattacg aacatcagta atgccgtcta
     7201 atataataaa taaaggtgtt ttgccttcat tataacaaaa atctacaatt tcttgaaaat
     7261 tgtaataagc tatgagttgt atagcggcca ctacgccttg gtgtggttgt ttattggcta
     7321 aataatctaa ttttatttca ggtacatact gcacataaac atctttctcc tttgcttttt
     7381 gaattatttt tttgaccgca tcaccgtgtg cttgttttga aatgtaaatt ttctcaaaat
     7441 tagtttcttc ctcaaaagcc tctaaaatgc tgtttcttcc aatgatatat tgtgcttgca
     7501 tattgcaaag ataagatttt ctaaagcatt attttgttat caaaattcta aagaatttca
     7561 aaacattaaa aacgaatgtc gcctttgcat atttcttcgt gaaagtcttt atccaataaa
     7621 atatattgtt tgtttttgag tacatataca tcatctttga tgatatttat atcaaagcct
     7681 tcttttttta attcactttt ttgatgtttg aaagtgcttg taatggcagc atctctagca
     7741 attcttacaa aaataggaac tgcatacgca ggtaattcat tagataataa acgatacaga
     7801 ttatccatat ctaatgcttt attttctttt aaaattattt tagccattcc tgctcgacca
     7861 ttgcaacctg gaatttcaac gccgaaaact atactttctt caacattatc tatcaaattg
     7921 atgatgcctt ctacttcttg agtagaaaca ttttcacctt tccacctaaa agtatcgccc
     7981 aatctatcta caaattgtgt atgaccaaat cccatatctc taaccaaatc attggtatta
     8041 aaccaagcat cgccttttgt aaaaacattc cttaaaatag tctgttctgt tttgcttttt
     8101 tcagtataac catcataagg ataacgttcg ttaatttctc caataagtaa accaacgcca
     8161 ttggttttaa ctttttgcat aaagccgttt ttgtctaatt taggtgctcc tttttcaata
     8221 tcatatttta caattgcata gcttgtgata gaaaatccca tagtttggtc gaggttaaag
     8281 acattgaaaa atgcaatgtt tccttcactt gaagcataaa attcggctac ttgttctatg
     8341 ttgaaacgct gtttgaattt ttgccaaata tctggtcgca aaccatttcc aatcattttc
     8401 tttaaagtgc tattcttttc taatggattt tcaggtgtgt tcaataaata tttacacatt
     8461 tctccaacat aaccaaatcc tgtaacttga tatttatgaa tatctttcca aaaatccgaa
     8521 acactaaatt tattttttat aacaattgca gcaccgccag ccacaactga agaccagcaa
     8581 accaccatag cagtagcatg aaaaaatggt aatggaacat ataaaatatc cttttcattt
     8641 aatcgcaaac tcgttaaacc aaaagcagaa tagcctttta gccatcgtcc atgactgata
     8701 atagaagctt ttggtaagcc tgtagttccc gaagtataaa tatacatggc tgggtcttta
     8761 gcaaatattt ttggttggta atttggtttt tgattggaat attggaaaga ttttatgtca
     8821 aaaattttca actgacttga tgttcttttg agttcatcgg caatcgaaaa aacaagattg
     8881 tttggaaagt ttaaggtgtt ttttatttca ttataattgt taattaagtt ttggtcaatt
     8941 aataataatt ttggttgagc taattgaata ctatgaatca aaggcttgtt tcgttgagct
     9001 gtgtttaaaa gtgcagcaat tccaccaatt ttggataatg ccatacttac agccaataat
     9061 tcaggtctat tttctaataa aacagcgaca acatcacctt tttttattcc gattgcaacg
     9121 aagtgattgg ccaaacgatt gcaccattca tcaaattctt gataagtcca ttttctatct
     9181 aaataataga
//
LOCUS       DAOQCW010000105         9126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_2895_length_9126_cov_5.447360, whole genome shotgun sequence.
ACCESSION   DAOQCW010000105 DAOQCW010000000
VERSION     DAOQCW010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9126
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2895_length_9126_cov_5.447360"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="PLS16_09225"
     CDS             <1..1080
                     /locus_tag="PLS16_09225"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNJ61303.1"
                     /translation="YELKGHKWFTSAITADMAFTLASTEQPQDGKRAPLSLFYLPIRN
                     EDGSLNGIEVEALKDKLGTKALPTAQLVLTGAKARMVGEQGKGVRTISTLFNVTRIYN
                     TVSAVSYMRRAFALSEAYGYIREAFGKKINQHILFKKSLMDLEIAYQSNTLLALFLTQ
                     LLGKEDCKAATENEEAVLRVLTPIAKLYTAKASIAAASEHVEMFGGLGYLEDTNIPVM
                     LRNVQTLSIWEGTTNVLSLDMLRAMEKENGLSAFRTFATTLLDSIKHEDLVEYKQIIS
                     DKLNVLLHFTKNITDKNALEGCARDIAFYIGDLTIGLLWLEFIQKCPKEKYIKAIKYW
                     TSFKMNEKSLELYQILLAENKEQLV"
     gene            1154..3304
                     /locus_tag="PLS16_09230"
     CDS             1154..3304
                     /locus_tag="PLS16_09230"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNJ61304.1"
                     /translation="MQHNKFLSEQELAKDFVQNTFVNVFVTGKAGTGKTTFLKSIQET
                     TQKKMAVAAPTGVAAINAGGVTLHSLFQLPIGAFLPTKKHFSEIATNINSLFSNLKMG
                     KAKLDLINELELLVIDEVSMLRCDTLDCIDIILKTIRKNQQEAFGGVQVLFIGDMFQL
                     PPVIKDEEWNLLSQFYNTPFFFSAQVMQECHYVHIEFTEIFRQTDNHFIRLLNNVRNN
                     EMEEEDFIKLNERFQPDVIEELDNFITLTTHNWKADKINQNQLAKLDGDIFTFNGSLE
                     GDFNDKALPTEMELNLKVGAQVMFVKNDIKPEKKYYNGKLAQIIKISKEDIVVSFFDD
                     KEEYTLEKAKWDNVRYEYNAEDDKINEKIIGSFSQFPIRLAWAVTIHKSQGLTFDRAV
                     IDAGQSFAAGQVYVALSRCRSLEGVFLLTKIAPNAIKSDERIVVFTQDSVSKISKLVD
                     ELDFHKFEFAKAEIYKCFSWDKYLEIIEDYRLFAIEKKLPEKEVILEKIAAVKVTVSE
                     INTISVKFNKWLEQNFALDTQATLNAVIKEKLEKSIVYFGQIIAQNILSVLDTIIDEL
                     KVKKNVKGFVKATESVHKTFLTKLHEIEGFRFLDERLYQEESHYTRQYNESSSNKLDT
                     KEVSLMMYKSDKTIDEIAKLRDLTTTTIEGHLAHFIQLGKLSVYDFIEKSDFETIKSA
                     TQELGTQQLKPLKDFFEDKFTYGQLKMAVAGLNR"
     gene            complement(3319..4380)
                     /locus_tag="PLS16_09235"
     CDS             complement(3319..4380)
                     /locus_tag="PLS16_09235"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HNJ61305.1"
                     /translation="MTIQQITTLLGEEQARFLLEHQSKTISKESLHLPSPTFVDDIFM
                     QSNRNPQTLRSLQQIYGTGRLANTGYVSILPVDQGIEHSAGASFAPNPIYFDPENIVK
                     LAIEGGCNAVASTYGVLGSVARKYAHRIPFIVKINHNEFISYPNTFDQIMFGTIKDAW
                     NMGAVAVGATIYFGSEESNRQIVEVAKAFEYAHQLGMTTILWCYLRNNGFKKDGVDYH
                     TAADLTAQANHLGVTIQADIIKQKLPTNNGGYTAINFGKTHKKVYESLTTDHPIDLTR
                     YQVANCYMGRAGLINSGGESKGQTDLAEAVETAIVNKRAGGMGLISGRKAFQKDMKIG
                     VELLNAIQDVYLNNDVTIA"
     gene            complement(4483..5379)
                     /locus_tag="PLS16_09240"
     CDS             complement(4483..5379)
                     /locus_tag="PLS16_09240"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNJ61306.1"
                     /translation="MYIAFLVILFFVGIIGQAYKVVPNNFFKKINRFIIYIPLPAIAL
                     TKIPQLHIDKQVILPILSVWIIFFVAILFFYLIGKYLKLSKATIACLVLSCGLGNTSF
                     VGFPVLTQLYGSDSLQYAIFLDQPGSFLVMSSLGILVASYASTGIFNIQTIFRKLFTF
                     PPFLCFLIALIIPKNFFNVSINQILEAIGSIMIPLAMLSLGLQFKLEFKNTPWRNFAI
                     GVSYKLILAPLLIYLIYFVILGQDTYINKIAVMETAMPPMITSSIIASEYGLDEELAG
                     VLPTLGIILSIPSLAGWYYFLG"
     gene            5463..7157
                     /locus_tag="PLS16_09245"
     CDS             5463..7157
                     /locus_tag="PLS16_09245"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNJ61307.1"
                     /translation="MLKNIITSFYLILVLFACTRLKPIDTSIKNISDTTIVDKNNFVP
                     PLKLPFLLAASFGEPRPNHFHTGIDIKTNQKEGYPVFVIDDGLVVRIKISPYGYGKAL
                     YVQHNNGLTSVYAHLQKFNEEIEKYINSQQYKMQINELDLVLSDTVFAFKKGTIIAYS
                     GNTGGSSAPHLHFEIRDTKTEYPLSPLDFYPKNSFVDTIPPQINNVIIKEYDGLKGIL
                     VNEKVVKIDSKKKDTLIVKIAQPFFTFSLEGFDKYDLSNTKNGIDKTVVFQDNKQLFT
                     YEIKNLDFAKARMCNVFFDYSLYRKNNTYAYNCFQEEGNTYPIYPTKNIEKYALHLED
                     TLLFKVDCYDYKNNCTEIWLKTIYEPKVFFIHEQYENAKPIIFGKADSIFINNFKLKW
                     QANSFYQNGLLEYKKVGEQYQISNFRNSTPLQSAATIQLKLSSNYKKDKLNMVLINES
                     KDTIYLKSKIEDDNIYADTRELGTLYLDYDTIAPIISNIHVDTIHKKITAKIVDSQTG
                     IQSYSMYINSIWVPVYYDAKEHIIEYKFDSNFPKGINEIKIAVSDNNGNVTSLIMQ"
     gene            7227..8273
                     /locus_tag="PLS16_09250"
     CDS             7227..8273
                     /locus_tag="PLS16_09250"
                     /inference="COORDINATES: protein motif:HMM:NF015686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61308.1"
                     /translation="MMDNLMNHPGVIAGFAVIVVAMLVLDLGIFNKKSHEIKNKEAIG
                     WSVLWISLSMIFSGVIYHFMGVEKFAQFQSAYWIEKALSVDNLFVFILVFQFYKVKKE
                     YQHKVLFWGIIGALIMRAIFIFAGVGLIKLTYLPPFELFGAKFMLSHASDVVPNYDLK
                     EFYRPNVVLSIFGFFLIYAGITSWFANDNEDAEKDFSSNLGAKFVNRFYKVKKQYFGD
                     KFFVVEDGIRYATLLFVVLAVIEFTDLIFAVDSIPAIFAIAPDDPMILYTSNIFAILG
                     LRSLYFLLANFMYMFSKLKYGLAIILTFIGFKMVFAPFLHVPSVVSLGIVGGILILSV
                     AVSLLFPEKKPNSI"
     gene            complement(8270..8827)
                     /locus_tag="PLS16_09255"
     CDS             complement(8270..8827)
                     /locus_tag="PLS16_09255"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNJ61309.1"
                     /translation="MANNFSEIKKGSLLLSEPFMEDPNFKRTVILITEHEKDGTLGLI
                     LNRPTIFTVNQMIGNFPHFSAFMNYGGPIDPERLNFLHAYPDLISDSFEIIPNVYWNG
                     NFEELKDNVKTKKVLPQNILFFSGYAGWEYEQLKEEIKENSWIICNNYKDIFKANKYM
                     WKEILHEMGGENKLIAEFPTHPSLN"
     gene            complement(8912..>9126)
                     /locus_tag="PLS16_09260"
     CDS             complement(8912..>9126)
                     /locus_tag="PLS16_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61310.1"
                     /translation="EFPKKEKTTKEKVAKEKKVKAEKPAKVKEEKAVKVKEEKAPKVK
                     EEKPAKVKEEKAPKVKEEKQPKTMTE"
BASE COUNT         3247 a         1222 c         1486 g         3171 t
ORIGIN      
        1 tatgaactta aaggacataa atggtttact tctgcaatta ctgcggatat ggcttttaca
       61 ttagcatcta ccgaacaacc gcaagatggc aaacgtgcac cactttctct attttatttg
      121 ccgattagaa atgaagatgg cagtttaaat ggtattgaag ttgaagcact gaaagataaa
      181 ttaggaacaa aagcattgcc tacagctcaa ttggttctaa ctggtgcaaa agctagaatg
      241 gtgggcgaac aaggcaaagg tgtaagaacg atttctactt tatttaatgt tacaagaatt
      301 tacaacacag tatctgcagt ttcatatatg cgtcgagcat tcgcactttc tgaagcttat
      361 ggatatattc gagaagcttt tggaaaaaaa atcaaccaac atattttatt taaaaagagt
      421 ttaatggatt tagaaattgc ttatcaaagt aatactttgt tggcattatt cttaacacaa
      481 ttattaggga aagaagattg taaagcggct actgaaaatg aagaagcagt tttgcgtgta
      541 cttacaccaa ttgcaaaatt atatacagca aaagcttcaa ttgcggcagc ttcagaacat
      601 gtggaaatgt ttggtggttt gggttatttg gaagatacaa atattcctgt aatgctcaga
      661 aatgttcaaa ctttaagtat atgggaagga acaacaaatg tactttcttt agatatgctt
      721 agggcaatgg aaaaagaaaa tggcttatcg gcatttagaa cattcgctac aacattatta
      781 gattctatta agcatgaaga tttggtggaa tataaacaga ttatttccga taaattgaat
      841 gttttattgc attttacaaa aaatattaca gataaaaatg cattggaagg ttgtgctaga
      901 gatatagcat tttacattgg agatttaacc atcggtttgt tatggctgga atttatacaa
      961 aaatgcccta aagaaaaata tattaaagcg attaaatatt ggacatcatt taagatgaat
     1021 gaaaaaagtt tagaattata tcaaatctta ttagctgaaa ataaagagca attggtgtaa
     1081 ttgtaactat tatatagctg caatattcga aacaaatgga tactttttta tacgaaataa
     1141 gtatctttag aaaatgcagc acaataagtt tttatcagaa caagaattag ccaaagattt
     1201 tgtacaaaat acttttgtaa atgtatttgt aactggaaaa gctggtacag gaaagactac
     1261 ttttttaaaa tctattcaag aaaccactca aaagaaaatg gctgttgcgg ctccaactgg
     1321 tgtggctgca ataaatgctg gaggcgtaac tttacattct ttatttcaat tgccaatagg
     1381 tgcttttttg cctactaaaa aacatttttc tgaaattgct accaatatta attcgctgtt
     1441 ttctaacctg aaaatgggca aggcaaaatt ggatctgatt aatgaattgg aacttttggt
     1501 aatagatgaa gtaagtatgt tgcgttgtga tacattggat tgtattgata ttattctcaa
     1561 aacgattagg aaaaaccaac aagaagcttt tggtggtgta caagttttat ttattggaga
     1621 tatgtttcaa ttgccaccag ttattaaaga tgaagagtgg aatttattga gtcaatttta
     1681 taatacgcca tttttctttt cagctcaagt gatgcaagaa tgtcattatg ttcatattga
     1741 atttacagaa atatttagac aaacagataa tcattttatt cgtttattga ataatgtacg
     1801 aaataatgag atggaagagg aggattttat taaactaaat gaacgttttc aacctgatgt
     1861 gatagaagag ttggataatt ttattactct aacaacacat aattggaaag ctgataaaat
     1921 taatcaaaat cagttggcaa aattagatgg cgatattttt acttttaatg ggagtttaga
     1981 aggcgatttt aatgacaaag cactgccaac ggagatggaa ttaaatttaa aagttggagc
     2041 tcaagtaatg tttgttaaaa atgatattaa accagagaaa aaatactaca acggaaaatt
     2101 agctcaaatc ataaaaattt cgaaggaaga tattgttgta tcgttttttg acgataaaga
     2161 agaatatact ttagaaaaag caaaatggga taatgttcga tatgaataca atgcggaaga
     2221 tgataaaata aatgaaaaaa ttattggaag tttttctcaa tttccaattc gattggcttg
     2281 ggctgtaact attcataaaa gtcaaggttt aacttttgat agagctgtaa ttgatgcagg
     2341 acaatcgttt gctgctggac aagtgtatgt ggcactaagc cgttgtcgaa gtttagaagg
     2401 tgttttttta cttacaaaaa ttgctccaaa tgcaataaaa tccgatgaaa gaatagtcgt
     2461 ttttactcaa gattcagtgt caaaaatttc taaattagta gatgaattag attttcataa
     2521 gtttgaattt gcaaaagctg aaatttataa atgctttagt tgggataagt atttagaaat
     2581 tattgaagat tatcgtcttt ttgcgattga aaaaaagttg cctgaaaagg aagttatctt
     2641 ggaaaaaata gctgctgtca aagtaaccgt ctcagaaatt aatactattt cagttaaatt
     2701 taataaatgg ttggaacaaa attttgcttt ggatacgcaa gccacattaa atgcagtgat
     2761 taaagaaaaa ttagaaaaat ctatagttta ctttggacaa attattgcac aaaatattct
     2821 ttcagtttta gacactataa ttgacgagtt gaaggtaaaa aagaatgtta aaggctttgt
     2881 aaaagcaaca gaaagtgtac acaaaacgtt ccttacaaaa cttcatgaaa ttgaagggtt
     2941 tagattttta gatgaaagat tgtatcaaga ggaatcacac tatactcgac aatataatga
     3001 atcatctagc aataaattag ataccaaaga agttagtttg atgatgtaca aatccgataa
     3061 aaccattgat gaaattgcaa agttaaggga tttaacaaca acaacgattg aaggacattt
     3121 agcacacttc atacaactag gcaaattgtc tgtttatgat tttattgaaa aatcagattt
     3181 tgaaactatc aaaagtgcta ctcaagaatt aggcacacaa caacttaaac cattgaaaga
     3241 tttctttgaa gataagttta cttatggtca attaaaaatg gcagttgctg gattgaatag
     3301 gtagatgaaa cgaaatgctt atgcaattgt tacatcatta ttcaaataaa catcttgaat
     3361 tgcatttaat aattctactc caattttcat atctttttgg aaagcttttc tgccagaaat
     3421 taagcccatt ccacctgctc gtttatttac aattgctgtt tctactgctt ctgcaaggtc
     3481 agtttgtcct ttactttcac caccagaatt aattaatcca gctctgccca tataacaatt
     3541 ggctacttgg taacgggtta aatcaatagg atggtcagtt gtcaatgatt cataaacttt
     3601 tttgtgggtc tttccaaagt ttattgccgt atatccacca ttattggttg gtagtttttg
     3661 tttaatgata tcagcttgga tggtaacgcc caaatgattg gcttgagcag ttaagtccgc
     3721 agccgtatga taatcaactc catctttttt gaagccatta tttcttaaat aacaccataa
     3781 aatagtagtc atacccaatt ggtgagcata ttcaaaggct tttgccactt caacaatttg
     3841 tctgttgctt tcttcagaac caaaatatat agttgcacca acagcaacag cacccatatt
     3901 ccaagcatct ttaattgttc caaacataat ttggtcgaaa gtatttggat aagatataaa
     3961 ttcgttgtgg ttgattttta ctataaatgg aattctatgt gcatattttc tagccacact
     4021 acccaaaaca ccataagttg aagcgacagc attacaacca ccttcaattg ctaattttac
     4081 gatattttca gggtcaaaat aaataggatt tggagcaaaa cttgcaccag cactatgttc
     4141 tataccttga tcaactggca aaatagaaac atatccagta tttgctaatc tacctgttcc
     4201 ataaatttgt tgcaaacttc ttaaagtttg cgggtttcta ttgctttgca taaaaatatc
     4261 atcaacaaaa gttggagaag gcaaatgtaa actttctttg gagatagttt tggattgatg
     4321 ttctaataaa aatctagctt gttcttcgcc taaaagtgta gtaatttgtt gaatagtcat
     4381 gttacttatt ttgttatgca aagataagga tacttttaat tgttttattg gaattgctca
     4441 ataaaataaa taacaaatca atttttaatg tggaaagtag ctttaaccta aaaaataata
     4501 ccagccagcc aatgaaggta ttgataaaat aataccaagt gtaggcaaaa cacctgctaa
     4561 ttcttcatct aatccatatt ctgatgcaat gatagatgaa gtaatcattg gaggcatcgc
     4621 agtttccatc actgcaattt tattaatata cgtatcttga cctaaaatta caaagtagat
     4681 tagataaatc aataaaggtg ctaatatcaa tttgtaacta actccaattg caaagtttcg
     4741 ccaaggtgta tttttaaatt ctaacttaaa ttgcaaacct aatgataaca ttgccaaagg
     4801 aatcataata cttccaatcg cttctaatat ttgattgata gatacgttaa aaaagttctt
     4861 tgggattatt aatgcaatta agaaacaaag aaacggcgga aatgtaaata gttttctgaa
     4921 aattgtttga atattaaaaa taccagtact tgcatacgaa gcaactaata ttcctaatga
     4981 tgacattact aaaaaactgc ctggttggtc taagaaaata gcatactgca aactatctga
     5041 gccatataat tgtgttaata caggaaagcc aacaaaggaa gtattgccca agccacaact
     5101 gagtactaaa caagcaatag ttgccttgct gagtttaagg tattttccta ttaaatagaa
     5161 aaaaagaatg gcaacaaaaa atattatcca aacagataaa ataggtaaaa taacttgctt
     5221 atcaatatgt aattgtggaa ttttagttaa tgcaattgca ggcaaaggaa tataaatgat
     5281 aaatcgattg atttttttga aaaaattatt tggaacaact ttataagctt gtccaataat
     5341 tccaacaaaa aataaaataa ctagaaatgc aatatacata gtttgtcaaa gctagaaatc
     5401 tttttgtatt ttacagatta tgaatttttt aattttattg aaaatcaatc gtatttttga
     5461 atatgctaaa aaatattatt actagctttt atttaatcct tgttttattt gcatgtactc
     5521 gattgaaacc aattgacaca tcgattaaaa atatttcaga tacaacaatc gtagataaaa
     5581 ataattttgt accaccttta aaattgccat ttttattggc agcaagtttt ggagaacctc
     5641 gtcctaatca ttttcatact gggattgata taaaaacaaa tcaaaaagaa ggttatccag
     5701 tatttgtaat tgatgatggt ttggttgttc ggataaaaat atcaccttat ggatatggga
     5761 aagctttgta tgtgcaacat aataatggtt tgactagtgt ttatgctcat cttcaaaaat
     5821 ttaatgaaga gatagaaaaa tatataaaca gccaacaata taaaatgcaa atcaatgaat
     5881 tagatttggt tttatctgat actgtatttg ctttcaaaaa aggtaccatt attgcctatt
     5941 caggaaatac aggaggtagc tccgcaccgc atttgcattt tgaaattaga gatacaaaaa
     6001 ctgaatatcc tttgagtcca ttggattttt atcctaaaaa cagctttgta gatactattc
     6061 caccacaaat taataatgta attattaaag aatacgatgg acttaaaggt attttggtta
     6121 atgaaaaagt tgttaaaatc gacagcaaaa agaaggatac attgatagtg aaaatagctc
     6181 agccattttt tactttttcg ttagaaggtt ttgataaata cgacctatca aatacaaaaa
     6241 atggaataga taaaacagta gtatttcaag ataataagca actcttcact tatgaaatta
     6301 aaaacttaga ttttgcaaaa gctagaatgt gtaatgtttt ttttgattat tcattatata
     6361 gaaaaaataa tacttatgcc tataattgct ttcaggaaga aggcaatact tatccgattt
     6421 atccaactaa aaatatagaa aaatatgctt tacatttaga ggatacatta ttgtttaaag
     6481 ttgattgcta tgattataaa aataattgta ctgaaatttg gcttaaaaca atttacgaac
     6541 ctaaagtatt ttttattcat gaacaatatg aaaatgcaaa acctataatt tttggtaaag
     6601 cggatagtat ttttataaat aattttaaat taaaatggca agccaatagt ttttatcaaa
     6661 atggtctatt agaatataaa aaagtaggag agcagtacca aatttcaaat ttcagaaatt
     6721 ctacgccatt acaaagtgca gctacaatac aattaaagct gtcatctaat tacaaaaagg
     6781 ataaattaaa tatggttttg ataaatgaaa gtaaggatac aatttatctg aaatcaaaaa
     6841 ttgaagatga taatatttac gcagatactc gagaattggg tactctttat ttagattatg
     6901 atacaatagc tccaataatt agtaatattc atgtagatac tatccataaa aaaattacag
     6961 ctaaaattgt tgatagtcaa acaggcattc aaagttatag tatgtatata aattcaattt
     7021 gggtacctgt ttattatgat gcaaaagagc atattattga gtataaattt gactcaaatt
     7081 ttccaaaagg gattaatgaa ataaaaatag cggtaagtga taataatggg aatgtaacaa
     7141 gtttaataat gcaatgattt aaaataagta ttgcaatatt taacaaataa aaaaaatctt
     7201 tctatttatt tttgtggcaa ttataaatga tggataattt aatgaatcac cctggcgtaa
     7261 ttgcaggttt tgcagttatt gtagtagcaa tgttagtttt ggatttaggt atttttaata
     7321 agaaaagcca tgaaattaaa aataaagaag caataggctg gtccgtatta tggatttcat
     7381 tatctatgat atttagcggt gtgatttatc attttatggg tgtggagaaa tttgcacaat
     7441 ttcagtctgc ttattggata gaaaaagcac tttcggttga taatttattt gttttcattt
     7501 tagtatttca attttataaa gtaaagaaag aatatcaaca taaggtattg ttttggggca
     7561 tcattggagc attaattatg cgagctattt ttatatttgc tggtgtaggt ttaataaagc
     7621 ttacctattt gccacctttt gaattgtttg gtgcaaaatt tatgctttct catgcaagtg
     7681 atgtagttcc taactatgat ttgaaggaat tttatagacc taatgttgtc ttgtcaatat
     7741 tcggattttt cttgatttat gcaggtataa catcttggtt tgcaaatgat aatgaagacg
     7801 ctgaaaaaga tttcagtagt aatcttggag ctaaatttgt gaatcgtttt tataaagtta
     7861 aaaagcaata ttttggagat aaattttttg tagttgaaga tggtattcgt tatgcaactt
     7921 tattgtttgt tgttttagcc gttattgagt ttacagattt aattttcgca gtagattcta
     7981 ttccagcaat ttttgcgata gcacctgatg acccaatgat tctttataca tcaaatatat
     8041 ttgccatttt aggtttaaga tcattatact ttttattggc taactttatg tatatgttta
     8101 gtaagttaaa atatggttta gctataattt tgacctttat aggatttaaa atggtatttg
     8161 ctccattttt acatgttcct tccgttgttt ctttaggaat tgttggaggt attttgatat
     8221 tatcagtagc tgtttcttta ttatttcctg aaaagaaacc taatagcatc taattcagtg
     8281 aaggatgagt tggaaactca gcaattagtt tattttcacc acccatttcg tgtagaattt
     8341 ctttccacat atacttattc gctttgaaaa tgtctttgta attattacaa attatccagc
     8401 tattttcttt aatttcttct ttcagttgtt catattccca accagcataa ccactgaaaa
     8461 ataaaatatt ttgagggagt actttcttag tttttacatt atctttgagt tcttcaaaat
     8521 tgccattcca ataaacattt ggaattattt caaaactatc agaaattaaa tctggatagg
     8581 catgtaaaaa gtttaatctt tcagggtcaa ttggaccacc gtaattcata aaagcactaa
     8641 agtgtgggaa gtttccaatc atttgattga cggtaaaaat agttggacga tttaaaatta
     8701 atcctaatgt tccatctttt tcatgttcag taattagtat caccgtcctt ttgaaatttg
     8761 ggtcttccat aaaaggttca gatagtaaca gagagccttt ttttatttca gaaaaattgt
     8821 ttgccataat aatatgaata taagcattct tttggaataa aaaaagtgta gccaacgact
     8881 acacttttat attctaaaaa aaacaagaaa attattcagt cattgtttta ggttgttttt
     8941 cttcttttac ttttggagct ttttcttcct taactttagc tggtttttcc tcttttactt
     9001 taggtgcttt ctcttctttt accttaacag ctttttcttc ttttacttta gcaggttttt
     9061 cagcttttac cttcttttct ttagctactt tttcttttgt tgtcttttct tttttaggaa
     9121 attctt
//
LOCUS       DAOQCW010000106         9079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_2915_length_9079_cov_5.389738, whole genome shotgun sequence.
ACCESSION   DAOQCW010000106 DAOQCW010000000
VERSION     DAOQCW010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9079
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2915_length_9079_cov_5.389738"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PLS16_09265"
     CDS             <1..402
                     /locus_tag="PLS16_09265"
                     /inference="COORDINATES: protein motif:HMM:NF020091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HNJ61311.1"
                     /translation="DAKKEINDQDLLEIMGINADSRMFHVVSHAVSCGNQHQSVAKIE
                     LSRDGKTYQSESEGNGPVDATLKAVDKIMGIPVKLEEYLVQAVTGGSNDVGKVHIQIS
                     KDGVFYYGFGADTDVIYASAMAYVDALNKSI"
     gene            complement(512..1858)
                     /locus_tag="PLS16_09270"
     CDS             complement(512..1858)
                     /locus_tag="PLS16_09270"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HNJ61312.1"
                     /translation="MLPEKIQQKLQNILTDKNFSFVAKGSFTTLLTLGFVQGLRFISG
                     IIIGRYYGADASGKLTLVITVMSIAGILCNFGIKDAIQRLIPESKVKYNNKAAYDYFI
                     LGMLLMCINWLLIGMVLYFIAPYLAIYWDEPSLTPLFRWSALFVLPAVLGDYFFFSLK
                     AGLKINTANVSLIIPTILRVVLLAIVTIYFYNIYNPIYMHWATMATLPFLFSIYPIYK
                     NFYIASKSDLRIFEVKRQQIFPLAFPMFITYFAFLVNNNADVFILKSLGITTANVGIY
                     KTANNIALISATLLVALNTTVQPKMTQLYFKHELDELRKVTQKSTKLIFWLSLPIYIL
                     LFGLAPYIMQIYGDSFLPGTIPLMIMVIGQLVNTATGPVAQLLNATGFHKQFTYMSLM
                     GAFLNIGFNFILIVPFGIIGASIAASLSLIIWNLIGVWYIYKKFGFIIAYFPKFGK"
     gene            complement(1881..2441)
                     /locus_tag="PLS16_09275"
     CDS             complement(1881..2441)
                     /locus_tag="PLS16_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61313.1"
                     /translation="MKKLPIILYFILQFLLFGCKTSNSKTGEAIELFNIKPLIEKDIT
                     YNQANNVKQKKVITVNQKQETTNVSKIDWAKELQIVLNADINKKDWEDKFTKQTTFDS
                     LENIETTKYLSFNSKVPIKKFIVKKSISSDTIHEIYIEKIIQSGLFSNDQIITYRPTA
                     YLKVASVQKAIFMDDFISEVEIYFIR"
     gene            complement(2472..4010)
                     /gene="gpmI"
                     /locus_tag="PLS16_09280"
     CDS             complement(2472..4010)
                     /gene="gpmI"
                     /locus_tag="PLS16_09280"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNJ61314.1"
                     /translation="MNLNNNQKKAFLIIMDGWGINRNASNSAIEAAQTPFIDSLYTQY
                     PNAVLLTHGEYVGLPYDQMGNSEVGHMNLGAGRVVYQDLLLINKKVEKGEFANSKALK
                     DAFEYAKQNNKKVHFLGLVSDGGIHSHTNHLKALIQLANEQGVQNCFVHAFTDGRDTD
                     PKSGLGYLSDLESFCQNTHTKIATICGRYYAMDRDKRWERIQLAYNLLVHGHGKTFAT
                     VQDAIQDSYNNNITDEFILPAIITNNASQINSNIEKDDVVICFNFRTDRCREITQVLT
                     QQNMPEFDMKTLPLYYLTMTIYDEKFQQVIPIFGKENIQKSLGEVIADNGLTQLRIAE
                     TEKYPHVTFFFSGGREQAFEGEERIMIPSPKVATYDLKPEMSAYEIKDAVIQFIKEKQ
                     PHFICLNFANTDMVGHTGVFEAGIKAAETVNQCVEEVVKVALDFDYGILLTADHGNAD
                     LMINNDGSPNTAHTKNAVPLFYIDNQYKKELRPGKLADIAPTILHIMQLPIPEEMEGN
                     VLIY"
     gene            4073..4462
                     /locus_tag="PLS16_09285"
     CDS             4073..4462
                     /locus_tag="PLS16_09285"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HNJ61315.1"
                     /translation="MFIRKCILLFIFVLSIHYTEAQDVSNDIVQYIQSGNTKELAKYF
                     NKRIDVTINQTGNNYSSDQAELIIKNFLSAIPNKEFTILQKGSTEYNTRFIVGVLKSK
                     NEKYKTYILLKPFQKILYIQDIRFEKE"
     gene            4563..4724
                     /gene="rpmH"
                     /locus_tag="PLS16_09290"
     CDS             4563..4724
                     /gene="rpmH"
                     /locus_tag="PLS16_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNJ61316.1"
                     /translation="MKRTFQPSNRKRKNKHGFRERMKTKGGRAILNARRRKGRKKLSV
                     SDEMRGRNK"
     gene            complement(5103..5431)
                     /gene="rnpB"
                     /locus_tag="PLS16_09295"
     ncRNA           complement(5103..5431)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLS16_09295"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(5449..6153)
                     /locus_tag="PLS16_09300"
     CDS             complement(5449..6153)
                     /locus_tag="PLS16_09300"
                     /inference="COORDINATES: protein motif:HMM:NF013166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HNJ61317.1"
                     /translation="MQLILFHHAGGDKYAFQYLSQKLNQDFEIIYYELPGRNYRLNES
                     LLFDIHQIVDDAYNTILPYTQNDFVFIGMSMGALISFLVAEKLHQQNKNLPKHIILSS
                     RKSIEQYSATQLVANADDETFWNYVKSFGANIDGLLQHQELKNFYEPILRADFKALEY
                     FNHHYQNQLSTQLPISVSSLIGKDDIHIDIEGANSWNKFFTKNFDAKAFDGGHFFLYN
                     NDEAIEYIRNKVLSLD"
     gene            6301..7089
                     /locus_tag="PLS16_09305"
     CDS             6301..7089
                     /locus_tag="PLS16_09305"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNJ61318.1"
                     /translation="MAQAQTTTKKQSGLNSGIVILIATVLCNVIFYTVFAKQFDEQGH
                     HTNVLGLVYKGGFVVPFLLATFIICITFAVERLLTINKAKGTGDLDGFLRNIKSKLVS
                     NDINGALADCAKQQGSVANVVQAGLHKYAEMEKNTGLDAEQKVMNIQKEIEETTGLEL
                     PVLEKNMWILATIASVGTLIALFGTVLGMIKAFTGLAASGSPDPEVLATGISEALINT
                     AIGIITSAVAIIFYNLCTNMIDKLTYGIDEMGYSISQTFAAKNK"
     gene            7113..7748
                     /locus_tag="PLS16_09310"
     CDS             7113..7748
                     /locus_tag="PLS16_09310"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNJ61319.1"
                     /translation="MPKVKVARQSTFVDMTAMCDVAFLLLAFFILTAKFRPSELIQVQ
                     TPSSRAEHAIKDDLVTFTIDKEGKVYMAISDIDRKRQVIDKLFELEPQKYAGVAMTDE
                     KRKEFQGLEIVGVPVQNLPQVVSMPKEEIQRLKDAGQLSGIPIDSTNNQLKDWIMAVR
                     HVYVQYENKEKAPIAIKGDKNTSMKDITNLIDIFRKNDVYSFNLITTLKVN"
     gene            7770..8336
                     /locus_tag="PLS16_09315"
     CDS             7770..8336
                     /locus_tag="PLS16_09315"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNJ61320.1"
                     /translation="MADLDTSSGGGRGKHKGKVRSKKMSTRVDLTPMVDLAFLLIAFF
                     MLTTTLGKSVAMDLNMPKPTDAESEKTVVKESKVLNIILEKDSKIWYYNGTTVMGLQQ
                     TDFSPEGIRQVILNKHKFVDSKFGRDDKGDVQTIVLIKPTDDSKYEDLVGILDEMDIT
                     KTKIYSIQKPVTLEEEAIANGGTPAVAN"
     gene            8361..>9079
                     /locus_tag="PLS16_09320"
     CDS             8361..>9079
                     /locus_tag="PLS16_09320"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNJ61321.1"
                     /translation="MSETKYSKSSWDDIVFEGLNQDYGSYVLRKLINKNTLRAFVITT
                     IVFLLILLIAKLQVFDRLAKKEEKIVEVALTDVNLPPPPPVEEAPPPPPPPPPPPVRP
                     TIKMVELIAKKDELVQDEKVTKQEEVKETQISTKTIEGDKEAKASIIIPKEEGGKGPE
                     PQPQPEKPKAPEILIRSEVMPSFPGGPAELMKYLGKNINYPSLARENGLEGKVIVNFY
                     VDTDGSIKNPTIVKDPVGGGCA"
BASE COUNT         3282 a         1334 c         1488 g         2975 t
ORIGIN      
        1 gatgctaaaa aagaaattaa tgaccaagat ttattagaaa ttatgggcat taatgcagat
       61 tctagaatgt tccatgtggt aagtcatgct gtttcttgtg gaaatcagca tcaatctgta
      121 gcaaaaatag aattaagtag agacggtaaa acttatcaat cagagtctga aggaaatgga
      181 cctgttgatg ccacattaaa agcagtggat aaaattatgg gtattccggt aaagttagaa
      241 gagtatttag ttcaagcggt aactggcgga agtaatgacg ttggtaaagt acatatccaa
      301 atatccaaag atggcgtatt ctattatggt tttggtgctg atacagatgt tatttatgct
      361 tcagctatgg cttacgtaga tgctttgaat aaatctattt agaattctta ttgtttcaac
      421 aaaagagatt tagctgttag aatgacgatt aaattatacg ttgcgatatc catttattta
      481 aagtgggttg aatagatttt gctaataatt tttattttcc aaatttagga aaataagcaa
      541 tgataaatcc aaattttttg tagatatacc aaacgccaat taagttccaa atgattaaac
      601 ttaaacttgc agcaatactt gctccgataa ttccaaaggg tactattaaa atgaaattga
      661 acccaatgtt caggaaagct cccattaatg acatgtaagt aaattgctta tgaaagcctg
      721 ttgcattgag taattgtgcg actggacctg ttgctgtatt taccagctga cctataacca
      781 taatcattaa aggtattgta ccgggtaaaa aactatcgcc ataaatttgc atgatataag
      841 gtgcaagacc aaatagtaaa atataaatag gcaagcttaa ccagaagatg agtttcgttg
      901 atttttgagt aacctttctc aattcatcta attcgtgttt gaaatataat tgtgtcatct
      961 taggttgtac agtcgtattt aaagccacta acaaggtcgc acttatcaaa gcaatattat
     1021 tggcagtttt atatatacct acattggcag ttgtaattcc taaagatttt aaaataaaaa
     1081 catctgcatt attattaacc aaaaacgcaa aataggtaat gaacatcgga aaagccaaag
     1141 ggaaaatttg ttgcctttta acttcaaaaa ttcttaaatc acttttagat gcaatataaa
     1201 aatttttata aattggataa atactaaata gaaaaggtaa tgtagccata gttgcccaat
     1261 gcatatatat tggattgtaa atgttgtaga agtaaatcgt tacaatggct aataatacaa
     1321 ctcttaggat tgttggaata attaaagaaa catttgctgt attaattttt aatccagcct
     1381 ttaaactgaa gaaaaaataa tcgcctaaaa cagctggcaa tacaaataag gcactccaac
     1441 gaaatagtgg tgtcaagctg ggttcatccc aatatattgc taagtacggt gcaataaaat
     1501 agagtaccat tcctatcaat aaccaattga tacacatcaa aagcatacct aaaataaaat
     1561 aatcataggc agccttattg ttgtatttta ccttactttc tggaatgagc ctttggatag
     1621 catctttaat tccaaaattg cataaaatac cagcaataga cattactgta attactaaag
     1681 taagtttgcc agatgcgtca gcaccataat acctaccaat gataatacca gatatgaaac
     1741 gtaatccttg aacaaatcct aaagtaagta gtgttgtaaa gcttccttta gcaacaaatg
     1801 aaaaattctt gtcggtaaga atattttgga gcttttgctg gattttttca ggtagcatga
     1861 atagcaaaag taagcatcat ttatcgaata aaataaattt ctacttcaga aataaaatca
     1921 tccataaaga tagccttttg tactgatgct acttttaaat aggcagtagg acgataagtg
     1981 atgatttggt cattagagaa taatccactt tgtatgattt tttcaatata gatttcatgt
     2041 atcgtatcgc ttgaaattga tttttttact ataaattttt taattggcac tttgctatta
     2101 aaagataaat attttgttgt ttcaatattt tctaagctat caaaagtggt ttgtttggta
     2161 aatttatctt cccaatcttt tttgttaata tcagcattta agacaatttg caattccttt
     2221 gcccaatcaa ttttagatac atttgttgtt tcttgttttt gattgacggt aattactttt
     2281 ttttgcttca cattgttagc ttgattataa gtaatatctt tttcaatcaa aggtttgatg
     2341 ttaaaaagtt ctattgcttc cccagttttt gaattagaag ttttgcatcc aaatagtaga
     2401 aattgtagaa taaaatataa tataataggg agtttcttca tcaaataaaa ttatataaag
     2461 actttaattt tttaatatat caatacgttt ccttccattt cttcaggaat tggcaattgc
     2521 ataatatgta aaatagttgg tgcaatatca gctaattttc caggtcttaa ttccttttta
     2581 tattgattat ctatataaaa taaaggaaca gcatttttgg tatgggcagt attcggagaa
     2641 ccatcattat taatcattag gtctgcattg ccatggtctg cagtaagcaa aatgccataa
     2701 tcaaaatcta aagctacttt tacgacttct tcaacacatt gattgacagt ttctgctgct
     2761 ttaattcccg cttcaaatac cccagtatgt cctaccatgt ccgtattcgc aaaatttaaa
     2821 caaataaaat gcggttgttt ttcttttata aattggataa cagcatcttt gatttcataa
     2881 gcactcattt caggtttcaa atcataagtt gctactttag gagatggaat cataatacgt
     2941 tcttcacctt caaacgcttg ttctcttcct ccagaaaaaa agaaagtaac atgaggatat
     3001 ttttctgtct ctgcaattct taattgtgtt aatccattat cggcaatgac ttcgcctaat
     3061 gatttttgga tattctcctt gccaaaaatg ggaataacct gctgaaactt ctcgtcataa
     3121 atagtcattg ttaaataata caaaggcaag gttttcatat caaactcagg catattttgt
     3181 tgtgtaagta cttgtgtaat ttcacgacat ctatcggttc tgaaattaaa acaaatgacg
     3241 acatcatctt tttcaatatt ggaattaatt tgggaggcat tgtttgtaat tattgctgga
     3301 agaataaatt catcagtaat attattgtta taggaatctt gaatggcatc ttgtacggta
     3361 gcaaaagttt ttccatgtcc atgtaccaat aagttataag ccaattgaat tcgttcccac
     3421 cttttatctc tatccattgc ataataacga ccacaaattg ttgcaatttt agtatgtgta
     3481 ttttggcaaa agctttctaa atcacttaaa tatcctaaac ctgacttcgg atcagtatca
     3541 cgaccatcag taaaagcatg cacaaagcaa ttttgtacac cttgctcatt cgctaattga
     3601 attagtgcct ttaaatgatt cgtgtgcgaa tgtatcccac catctgaaac taaacctaaa
     3661 aaatggactt ttttattatt ttgcttggca tactcgaaag cgtcctttaa agctttactg
     3721 ttggcaaatt ctcctttttc aactttctta ttaattaata ataaatcttg gtaaacgact
     3781 cttcctgcac ctaaattcat gtgtccaact tcagaattac ccatttggtc gtaaggtaaa
     3841 ccaacatatt ctccatgcgt aagcaataca gcgtttggat attgagtata taaactatca
     3901 ataaaaggtg tttgagctgc ttctatggca ctatttgatg catttctgtt gatgccccaa
     3961 ccgtccataa tgattaaaaa tgcttttttt tggttgttat tcaaattcat ttttttattt
     4021 ttgtataaag attggcataa aatatgctcc atctaaaacg aatttactta ttatgtttat
     4081 aagaaaatgc atattgctat ttatttttgt attatctatt cattatacag aagcacaaga
     4141 tgttagtaat gatattgttc agtatattca aagtggtaat acaaaggagt tagcaaagta
     4201 ttttaataaa cgtatagatg ttaccataaa tcaaacaggt aataattata gtagcgatca
     4261 agcggaattg attatcaaga attttttgag tgcaatacca aataaagaat ttactatttt
     4321 acagaaaggt agtacagaat acaatactcg ttttattgta ggtgttttaa aatcaaaaaa
     4381 tgaaaagtat aaaacctata ttttgctgaa gcctttccaa aaaatcttat atatccaaga
     4441 tattcgcttt gaaaaagaat agatttctaa gtagcttaca ttaatttatc gtttatttat
     4501 agaggatatt ttttttttaa cagaaaaata ctatctttgc attccgtttt aaaattaatt
     4561 gtatgaaaag gacattccaa ccatctaata gaaagagaaa aaataagcac ggttttagag
     4621 aacgtatgaa aactaaaggt ggtcgtgcaa tattgaatgc aagaagaaga aaaggaagaa
     4681 aaaaactttc tgtttctgac gaaatgagag gtagaaataa atagaagcga tagcaactat
     4741 accaaaatat actttaagta aaagagaacg cctaaaaagt aggaaaaaaa tccaactact
     4801 ttttaggcgt aaatcttttg ttcaaaatga atatctcaga attttttttc attttgaatc
     4861 agacaagtcc tcagtgcaaa tacatcaatt tgcatttgca gcacccaaga gattattcag
     4921 caacgcaaca gaaagaaact atattaagcg gttattgcga gaagcagttc gcaaaaataa
     4981 atctgaatta atagctccat ttacaaacaa aaccggaagt ttttttatta gctatgttca
     5041 taaagaaaaa ccgaatttca tgattattga aaatagccta ttaacgttat ttaataagat
     5101 aaaaaaatag caggtctata agccgagttc tgtaagtata aatacttttc tatcatttat
     5161 ctaggataat tatcgctaat tacctctatc catctaccca ccaacatccg gcgaacaacc
     5221 gtcaagcatt ggcttacatg atgtttcagc gtttggggtt tgcctagtta atatattact
     5281 ataaaaacat tttcactaca gccttacctg tacaattttc tgtggcactt tccatatatt
     5341 cctatataca gcagttagct gtcaaactgt tctgtgctgc tcagactttc ctcatttata
     5401 aatttataaa cgcgatagaa cgacctgcat ggacaaaatt acaaattttt agtctaatga
     5461 taatacctta tttctaatat attcaatagc ttcatcatta ttgtataaaa aaaagtgacc
     5521 accatcaaat gctttggcat caaaattttt agtaaaaaat ttattccaag aatttgcacc
     5581 ctcaatatca atatgaatat cgtctttgcc aattaaacta cttactgaaa taggtagttg
     5641 cgttgataat tgattttgat aatgatggtt gaagtattct aatgctttga aatccgctct
     5701 cagaattggt tcgtaaaaat tttttaattc ttgatgctgt aaaagtccat caatattagc
     5761 accaaatgat tttacataat tccaaaatgt ttcatcatct gcattcgcaa ctagttgggt
     5821 agccgaatat tgttcaatac tttttctaga agatagtata atatgcttag gtagattttt
     5881 gttttgttga tgtagctttt cggcaactaa aaatgaaatt aaagcaccca tactcatgcc
     5941 aataaataca aaatcatttt gagtgtaagg taaaatagtg ttataagcat catctacaat
     6001 ttgatgtata tcaaataata aagattcatt tagtcgataa tttctgccag gtagttcgta
     6061 ataaataatt tcaaaatctt gatttagttt ctgggataaa tactggaatg cgtatttatc
     6121 gcctcctgca tgatggaata aaatgagttg catattgtta aaaatacaat aaagagataa
     6181 attttgcgtt ataaaatgtg atagtttttt gaaaaaaagt ttaggtattt tcaaataaat
     6241 actatattta cccaaaatta aaaaaaatca aacatcaaat taaactaaaa attgataatt
     6301 atggctcagg cacaaacaac aacaaaaaag caaagcggtt taaattctgg cattgtaatt
     6361 ttaattgcaa cagtattatg taatgttatt ttttacacag tatttgcaaa gcaatttgac
     6421 gaacaaggtc accacactaa cgttttaggt ttggtgtaca aaggagggtt tgttgtacct
     6481 tttttattag caacatttat tatctgtatc acatttgcag tagaaagatt gttaacgatt
     6541 aataaagcaa aagggacagg agatttagat ggttttttaa gaaatattaa atctaaatta
     6601 gtttctaatg atattaatgg tgctttggct gattgtgcta aacaacaagg ttcagtagcg
     6661 aatgtagttc aagctggctt acacaaatat gctgaaatgg aaaaaaatac aggattagat
     6721 gcagagcaaa aagtaatgaa tattcaaaaa gaaattgaag aaactacagg attagaattg
     6781 cctgtattag aaaaaaatat gtggatttta gcaacgattg catcagttgg tactttaata
     6841 gcattatttg gaactgtatt aggtatgatt aaagcgttta caggattagc agcgagtggt
     6901 tcgccggatc cagaagtttt agcaacaggt atttctgaag cactaatcaa tacagcaatt
     6961 ggtatcatta cttcagctgt agccattata ttttacaact tatgtacaaa tatgattgat
     7021 aaactaactt atggtattga tgaaatgggt tatagtattt ctcaaacttt tgctgcaaaa
     7081 aataaataat cttaataaaa aaatagaata aaatgcctaa agttaaagta gcaagacaga
     7141 gtacatttgt ggatatgacc gcaatgtgtg acgttgcctt tttattgcta gcgttcttta
     7201 ttcttactgc aaaatttcgt ccatcagaat tgattcaagt tcaaactcca tcttctagag
     7261 cagaacatgc aataaaagat gatttagtta cttttacgat tgataaagaa gggaaagtgt
     7321 atatggcaat ttctgatata gatagaaaaa gacaagttat tgataagcta tttgaattag
     7381 aaccacagaa atatgctggt gtagctatga ctgatgagaa aagaaaagaa tttcaaggat
     7441 tagaaattgt tggagttcca gttcaaaatc tacctcaagt agtttcaatg ccaaaagaag
     7501 aaattcaaag gttaaaagat gctggtcaat tatcaggaat tcctattgat agtacgaata
     7561 atcagttgaa agattggatt atggcagtaa gacatgttta tgttcaatat gaaaataaag
     7621 aaaaagctcc aattgcaatt aaaggtgata agaatacaag tatgaaagat attactaact
     7681 tgatagatat ctttagaaaa aatgatgttt attctttcaa cttaattaca accttaaaag
     7741 taaattaaaa aaatctaaat aataattaaa tggcagattt agatacctcg tcaggcggtg
     7801 ggcgtggcaa gcacaaaggc aaagtccgaa gtaaaaaaat gtccacaaga gtggatttaa
     7861 cacctatggt ggatttagct ttcttgctaa ttgctttctt tatgctaaca acaactttag
     7921 gtaaatctgt agctatggat ttgaatatgc ctaaaccaac agatgctgag tctgagaaaa
     7981 cagttgtaaa agagtctaaa gtattaaata taatattaga aaaagatagt aagatttggt
     8041 actataatgg gacaactgtt atgggacttc aacaaactga ttttagtcca gaaggcatta
     8101 gacaagtaat attgaataag cataagtttg tagatagtaa atttggcaga gatgataaag
     8161 gggatgttca aacgatagtt ctaattaagc caacggatga ttcaaaatat gaagatttgg
     8221 tgggtatatt agatgaaatg gatattacta aaacaaaaat ctattctatc caaaaacctg
     8281 tgactttaga ggaggaagca attgcaaatg gtggaacacc agcagtagca aactaaatat
     8341 catttttata aaataataag atgagcgaaa caaaatatag taaatcaagt tgggatgata
     8401 ttgtttttga aggattgaac caagattatg gttcttatgt ccttagaaaa ttaatcaaca
     8461 aaaatacact tcgtgcattt gtcattacaa ctatagtatt tttattaatt cttttaatag
     8521 caaaattaca agtttttgat agattagcta agaaagaaga aaaaatcgta gaagttgctt
     8581 taacagatgt aaatcttcca ccaccaccac ctgtggagga agcaccacca ccaccaccac
     8641 caccaccacc gccaccagta agaccaacga ttaaaatggt ggagttgata gctaagaaag
     8701 atgagttggt acaagatgaa aaagtaacta agcaagaaga ggtaaaggaa acgcaaattt
     8761 ctacaaagac catagaaggc gacaaggaag caaaagcatc aataattata cctaaagaag
     8821 aaggaggaaa aggtccagag ccacagcctc aaccagagaa acctaaagca cctgagattc
     8881 taatacgttc agaggtaatg ccatcgttcc ctggaggtcc ggcagaatta atgaaatatt
     8941 taggaaaaaa tattaattat ccttctttag ctcgagaaaa tggattagaa gggaaggtta
     9001 ttgtaaactt ctatgtagat acagatgggt caattaaaaa ccctacaatt gtaaaagacc
     9061 cagttggagg tggttgtgc
//
LOCUS       DAOQCW010000107         8976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_2959_length_8976_cov_4.572245, whole genome shotgun sequence.
ACCESSION   DAOQCW010000107 DAOQCW010000000
VERSION     DAOQCW010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8976
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2959_length_8976_cov_4.572245"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..478)
                     /locus_tag="PLS16_09325"
     CDS             complement(59..478)
                     /locus_tag="PLS16_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61322.1"
                     /translation="MSIVKIIVIAILSFLLSFILPWYGFAIVAFIVGFFLAKYEGNSF
                     FAGFIGVGLFWLLYIIFLDTKNGNQLSGEVAQIFSDSLDTEISSALLIASASILAGII
                     GGLAAWAGSLFAQTNNIGRQHSKFSQRTSNKRFKLNI"
     gene            complement(482..2908)
                     /locus_tag="PLS16_09330"
     CDS             complement(482..2908)
                     /locus_tag="PLS16_09330"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNJ61323.1"
                     /translation="MHKIILASILSILTIVLIYFLNKSMGSTPQIGKFLSPTHGFWQN
                     TNVDDRVNYSYEIDCPSGNKATVVIDSIKIPHIFADNEVDLYYAQGFVQAQLRLWQME
                     FQTLYASGRLSEIVGDKALELDRFNRRIGMARAAKISVEEMSKDEQTKKIASAFSDGV
                     NAYIESLKYKDLPIEYKLLGYQPEEWSPYKSALLMKYMAFDLTYRNLDVEYSNALKLF
                     GEENFKTLYPDFPMPEDPIIPVGTKWNFKNIDSFGTKTVANSEALRADLYQNPMSKYM
                     PNKFLGSNNWAVSGSKTSSGKPILCNDPHLGLNLPSIWIAMQLNSKNMNVMGVTIPGA
                     PGIVIGFNDSISWGVTNASRDVINTYSVEYKDNHKKAYKIGNQYVDFTYQVDTIKIKG
                     QKPFIDSVRITVAGSIVYDENFGEQNDRKHLAVYWRAIEPSNELMTFYKLNHAKGHQD
                     YLEALNTFGCPGQNFVYADVNNNIAIKQQGHFMMRTSAEDGRFIEPLANINLNKLKSS
                     IPNNQNPYILNPSRGFVSSANQKPVDGNYPYSTGGDYENYRNRVINTELTAMRNIKPK
                     DMMALQGNNLSLLAKEILPTLLANLDTIKFKDSTSLAILKTFSKWNYFANYSYSGPSY
                     FYKWFETIERLTWDEFKSEKYSMIQPENYVLSNLIATRPNYELFDIEATTKTENAKDI
                     INLAFEETTKYFKEFYEQNKTTRWQIYKNTSVTHLAKIKAFSITEIPIGGYSNIVNAT
                     AKDAGASWRMIVDFAGGKPTAYGIYPGGQSGSPASKFYNNMVPLWANNEYYPLSFYLS
                     KSDALNSLKK"
     gene            3108..3740
                     /locus_tag="PLS16_09335"
     CDS             3108..3740
                     /locus_tag="PLS16_09335"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNJ61324.1"
                     /translation="MTNTFKSILVLIFACITLFVSSKELVQFSGYVKDNESLAGIPYA
                     AIYIKQQNKGTITNPDGFYNFVVAKGDTILIKSIGYKTYTVIIPFDIDGTSYSKDLLL
                     EREAYELKGVTIRPLPEPNQLRQAVLNLDIPNDLTELAQQTIANSILNDNFDKNMKYD
                     GAENYNYYAREQANYYYNRFGNQRPGISLTDPFAWAKFVKSIKDKKKKNK"
     gene            complement(3742..5328)
                     /locus_tag="PLS16_09340"
     CDS             complement(3742..5328)
                     /locus_tag="PLS16_09340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HNJ61325.1"
                     /translation="MNKREQQCHRAATEVFDIAIIGGGITGAGIALTAANNGWKTIII
                     DKNDFASGTSSRSAKMVHGGLRYLEQFQIGLVKEALHEREHLLKIYPHLVKPQPFFMP
                     IYKSKFSKLKLGIGLTLYDKLSGKKSKSPKHKSLNTSTIQQRFPQLKTDKMVGGFIYF
                     DAKTNDARLTNEVIQNAEDLGAVALNYADCLHFEKNNNLTHSIICKDNLSNKQFSIQA
                     KLFIIATGIWTDDTLKKYNADDVKKYMAPSKGIHLVVSANHLPKDCVMILPTSNGDGR
                     FLWCMPWEDNLNIIGTTDTDYKGNIEEIQVEQNEINYILDSVNAQLRNSKLTENDILS
                     VFAGIRPLLNDDDEDKKSNKRSRDYKIWWNNENTLTISGGKFTSFLSMAEHCLDTVKE
                     KLDYSSNQIQENIDILIAEKWQPLLNKYEGKYGDNNAKRIISIVQDFNDITLSNQDEK
                     YPYSDVEFIFFIRFQNALKIDDLLTRRTLVTYQMKNFDAEYVSKIATIMAKELDWNDT
                     QKLNEINDYKNAWQLMHSWH"
     gene            complement(5332..6987)
                     /locus_tag="PLS16_09345"
     CDS             complement(5332..6987)
                     /locus_tag="PLS16_09345"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNJ61326.1"
                     /translation="MNKDWWKWGDPAELKHMNDYPKAKAFLEDLWKTNFKHEYSPPNK
                     FSIEPLTEKKKDTLKDVFASLSSKKLSFRDEERIRVALGKSYYDVIRLCKDDKIVTPD
                     VVLAPTTNEDIEYIVKQANDHQVKLIPVGGGTNVVGALTMQENNSKNNLKATINLLNY
                     NKLIHIDEAHLTATFQAGILGPALEKILNEKGFTLGHFPQSFEYSTLGGWVVTRSAGQ
                     ESSYYGKIEDIVEQIKVVTPIGTLHSNDYTHDASGVNLLQLFVGSEGVFGVVSEVKVK
                     IKKLPKSSRWLTALFPSFEAGANYLNELTQNDLKPSVVRLSDANETLLFAKIGSEHNE
                     VSFIDNLKKEIQKFVLQWKNLTEPCVMIMRFDQNKYSVSPQILYAKSLIEKYKGIVAP
                     SIIGEKWAINRFKSPYMRDTFVEHGIYIDTMETLVHWKDVQQVHQALTKNLKKSKAFN
                     KEQGLFMAHISHVYPNAACMYFTLVTPMHRGNEIEQWHEIKELVSQTIVLNGGTISHH
                     HSIGADHQKWYLRNADPVGLQVLKSIKKILDPNGILNPGKLFT"
     gene            7210..>8976
                     /locus_tag="PLS16_09350"
     CDS             7210..>8976
                     /locus_tag="PLS16_09350"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNJ61327.1"
                     /translation="MNKFHLVWILGLLFTACSPKTTAQKSTSNATETNKEATTSPINA
                     NNTNEDVSNENLAKFKLPLDKTVRYGVLPNGLKYYIKANNKPENRVELRLAVNAGSNQ
                     EDDNQLGLAHFLEHMAFNGSTNFKKNDLVNFLEGLGVKFGAHLNAYTSFDETVYMLQL
                     PTDKKEVLDKGLLVLEDWASGISFDSLEINKERGVVVSEWRSGQGPDMRMLNKFLPTY
                     YYKSRYAERLPIGDTTILKNCAHERITTFYKDWYRPDLMAVMIVGSINVDSMEKEVIA
                     RFSDLKNPSIERQKIEYELPKHKETLVSIVTDDEASNTQAYIVYKHDKQDISTTEGFV
                     MHVANELINSMINNRLGELVEQSSSPFIQAGAAYDKETRKNDAFSDFIFMKKDRYKDA
                     LELILQENNRVKQHGFTASELARAKTELLKSYNDALKEKDKMESRTLIGELVSNFLNN
                     TPALGIEVESQLASQIISVIDLNTINEIAKSYITTDNRVVVITAPSAEKSKLPTEKEV
                     LAIVTKADNEKLEPYKDEVIDEPLIGTEIKAGKIVNQTTDKTFNITTYKLSNGATVKI
                     KPTDFKNDEIFFSAFSNGGTSLY"
BASE COUNT         3019 a         1386 c         1375 g         3196 t
ORIGIN      
        1 attcttttga aaatagaatt gcaggttatt aaaattaagt gtttttttta aaaaaaactt
       61 agatatttag cttaaatctt ttattagaag ttctttgact aaacttacta tgttgtcttc
      121 cgatattatt cgtttgagca aataaactcc cagcccaagc agctaaacca ccaataattc
      181 ctgctaaaat cgaagccgat gcaattaata gggcacttga aatttctgta tctaaactat
      241 ctgaaaatat ttgtgcgact tcgcctgata attgattgcc attttttgta tctaagaaaa
      301 taatatagag caaccaaaat aaaccaacgc caataaagcc agcaaaaaag ctattgcctt
      361 catattttgc taagaaaaat cctacgataa aagctacaat tgcaaaacca taccaaggaa
      421 gtataaagct caataaaaag ctcaaaattg cgataacgat aatttttaca atagacataa
      481 tttatttttt taagctgttt aatgcatcag attttgaaag ataaaaactc aatggataat
      541 attcattatt tgcccaaagt ggaaccatat tgttataaaa cttacttgca ggtgagccac
      601 tttgtccacc tggataaatg ccataagcag taggtttccc accagcaaaa tctactatca
      661 tacgccacga cgcaccagca tcttttgctg tagcattgac aatattacta taaccaccaa
      721 ttggaatttc tgttatacta aaagccttta ttttagctaa atgtgttaca gatgtatttt
      781 tatagatttg ccatcttgta gttttatttt gttcataaaa ttctttaaag tattttgtgg
      841 tttcttcaaa tgctaaattg attatatctt ttgcattttc ggtttttgtg gttgcttcaa
      901 tatcaaacaa ttcataattt ggtcgagttg caataagatt tgataataca tagttttctg
      961 gttgaatcat actgtatttt tcacttttaa attcatccca agttagacgc tctattgttt
     1021 caaaccattt gtaaaaataa cttggtcccg aataactata gtttgcaaaa taattccatt
     1081 tagagaaagt ttttaaaatg gctaatgaag ttgaatcttt aaatttaatc gtatctaaat
     1141 ttgctaataa tgttggtagt atttcttttg ctaacaaact caagttgtta ccttgtaaag
     1201 ccatcatatc ttttggtttg atattcctca ttgctgtaag ttctgtattg ataacacgat
     1261 ttctataatt ttcgtaatca cctccagttg aataaggata gtttccatca acaggttttt
     1321 ggttggcact cgaaacaaaa cctcttgaag gatttagaat atatggattt tggttatttg
     1381 gtattgatga ttttaattta ttgagattaa tatttgctaa aggttcaata aatcttccat
     1441 cttcagcaga tgttctcatc ataaaatgtc cttgttgttt gatagcgata ttattattga
     1501 catcagcata aacaaagttt tgcccagggc aaccaaaagt atttaaagct tctaaatagt
     1561 cttgatgtcc ttttgcatga tttaatttat aaaaagtcat taattcattt gatggctcaa
     1621 tagctcgcca ataaactgct aaatgtttac ggtcgttttg ctctccaaaa ttttcatcat
     1681 agactataga accagcaacc gtaattctta cagaatcaat aaaaggtttt tgacctttta
     1741 ttttgatggt atccacttga taagtgaaat ctacatattg attgcctatt ttatatgctt
     1801 ttttatgatt gtctttatat tctacggaat aggtattgat aacatctcgt gaagcattgg
     1861 taacacccca actaatgctg tcattaaagc cgattacaat tcctggtgcg ccgggaatgg
     1921 ttacacccat aacattcatg tttttgctgt ttaattgcat agctatccaa attgaaggca
     1981 agtttaaacc taaatgtggg tcattgcaaa gtataggttt gccgctactt gttttagagc
     2041 cagaaactgc ccagttatta ctgcctaaga atttattagg catatatttc gacattgggt
     2101 tttggtataa atcagctcta agtgcttcag agtttgcaac tgtttttgtt ccaaatgaat
     2161 ctatgttttt aaaattccat tttgttccaa caggaataat tggatcttca ggcataggaa
     2221 agtcaggata caaagtttta aaattctctt cgccaaataa ttttaatgca ttagaatatt
     2281 cgacatctaa atttctataa gttaaatcaa aagccatata tttcatcaat aaagctgatt
     2341 tatatggaga ccattcttct ggctgatagc ccaataattt atactcaatc ggcaaatctt
     2401 tatattttag actttcaata taagcattta caccatcact aaatgcacta gcaatttttt
     2461 tggtttgttc atctttcgac atttcttcga cgctaatttt agcagctcga gccattccaa
     2521 ttcttcggtt gaatctatct aattctaatg ctttatctcc aactatttca gataatcttc
     2581 ctgaagcata aagcgtttga aattccattt gccaaagtcg cagttgagcc tgaacaaatc
     2641 cttgtgcata atataaatcg acttcattgt ctgcgaaaat atgtggaatt ttaatgctat
     2701 caataaccac agttgctttg ttgccacttg gacaatcaat ttcataagaa taatttactc
     2761 gatcatctac attggtattt tgccaaaaac catgtgttgg agataaaaat tttcctattt
     2821 gaggtgtaga acccattgat ttgttgagga aatatatcaa tactattgta aggattgata
     2881 atatactcgc taaaataatt ttatgcataa aaaagtactt gcataaatgt aaaacttttt
     2941 agaatattat acaaagcaat ttcttatttc attaaaaagt attgttgcta tttaccaatg
     3001 ttattatgta actaatcacc caatactaac cttttttatc atgttatcaa ttgttaagca
     3061 gtcaagtatt ttaatatgtt gatatattta tgtgtatatt tgtattaatg acaaatactt
     3121 ttaaatctat attggttctt atttttgcat gtattacttt gtttgtatcg tctaaggaat
     3181 tggttcagtt ttcgggctat gtgaaagata atgaaagtct agcaggaatt ccttatgccg
     3241 caatttatat aaaacaacaa aataaaggaa ctattacgaa tccagatggt ttttataatt
     3301 ttgtagttgc taaaggagat actattttaa tcaaatctat tggctataaa acttataccg
     3361 taattattcc ttttgatatt gatggaacaa gttattctaa agatttatta ttagagcgag
     3421 aagcctatga actgaaaggt gtaaccattc gacccttacc agaacctaat caactaagac
     3481 aagcagtatt aaatttagat attccaaatg atttaacaga attagctcaa caaacgattg
     3541 caaattctat attgaatgat aattttgaca aaaatatgaa atacgatgga gcagaaaatt
     3601 ataattacta tgctcgtgaa caagctaatt attattacaa tagatttgga aatcagagac
     3661 caggtatttc tttaactgac ccttttgctt gggctaaatt tgtaaaaagt attaaagata
     3721 aaaagaagaa aaataaatag cttaatgcca actgtgcatt aattgccaag catttttata
     3781 atcattgatt tcattaagtt tctgtgtatc gttccaatct aattctttag ccataattgt
     3841 agcaatcttg gatacatatt cagcatcaaa atttttcatt tgataagtaa ctaaagttct
     3901 ccttgttagt aggtcgtcta tttttaaggc attttgaaaa cgaatgaaga agataaattc
     3961 aacatcagaa tatggatatt tttcatcttg attgcttaga gtaatatcat taaaatcttg
     4021 gacaattgaa ataattctct tagcattatt atcgccatat tttccttcat atttattaag
     4081 taaaggttgc catttttcag caattaaaat atcaatgttt tcttgaattt gatttgatga
     4141 ataatctagt ttttctttta ctgtatccaa acaatgttca gccatggaaa gaaaagaagt
     4201 aaattttcct cccgaaatgg taagtgtgtt ttcattattc caccaaattt tataatcacg
     4261 agaacgtttg ttgctttttt tatcttcatc atcatcattt agtaatggtc gaatacctgc
     4321 aaatacactc aaaatatcat tttcagtaag tttactattt ctaagttgtg cattaacact
     4381 atctaaaata taattaattt cattctgttc aacttgtatt tcttcaatat tacctttata
     4441 atcagtatct gtagtaccaa taatattcaa attatcttcc caaggcatac accataaaaa
     4501 tcgtccatca ccattagaag ttggtaatat cataacacaa tcttttggta agtgatttgc
     4561 agaaacgact aaatgaattc ctttgctagg agccatatat ttttttacat catctgcgtt
     4621 gtattttttg agtgtatcgt ctgtccaaat accagttgca ataatgaata attttgcttg
     4681 aattgaaaat tgtttattgg ataaattgtc tttacaaata attgaatgag ttaggttgtt
     4741 atttttttca aaatgaagac aatctgcata atttaaagcg actgctccca aatcttctgc
     4801 attttgaata acttcatttg ttaatcgagc atcatttgtt ttagcatcaa aataaatgaa
     4861 accacccacc attttatctg ttttcaattg aggaaaacgc tgttgaatgg tggaagtatt
     4921 taaacttttg tgtttcggtg atttgctttt tttgccactt aatttatcat ataaagtcaa
     4981 accaataccc aattttaatt tagaaaattt ggatttgtag ataggcataa aaaatggctg
     5041 tggttttact aaatgcggat atatttttag taaatgttcc ctttcgtgca aggcttcttt
     5101 aactaacccg atttgaaatt gctctaggta tctaagtcca ccatgtacca tttttgcgga
     5161 gcgagaactt gtgcctgatg caaaatcatt tttgtcaatg ataattgttt tccaaccatt
     5221 atttgcagca gtcaaagcaa tgcctgcacc agtaattcca ccaccaataa tagcaatgtc
     5281 aaatacttct gttgctgcac ggtggcattg ttgttctcgc ttgttcatta attaagtaaa
     5341 taattttcca ggattcaaaa tgccattggg atctaaaatc tttttaatgg atttaagcac
     5401 ttgcaaccca accggatctg catttcttaa ataccatttt tgatggtcag ccccaatact
     5461 atgatggtgt gaaattgtac caccattcaa aacaatcgtc tgacttacta attctttaat
     5521 ttcgtgccat tgctcaattt catttcctct gtgcattggc gttaccaaag taaaatacat
     5581 acaggctgca tttgggtaaa cgtgcgaaat atgtgccata aataaacctt gttctttatt
     5641 gaatgcttta gattttttta agttttttgt taatgcctga tgcacttgtt gtacatcttt
     5701 ccaatgaacc aaagtttcca tagtatcaat ataaattccg tgttctacaa aagtatcacg
     5761 catataaggc gatttgaatc tattgattgc ccacttttct ccgatgatag aaggagcgac
     5821 tattccctta tatttttcta ttaaactttt tgcatataaa atctgtggcg aaacgctata
     5881 tttattttgg tcaaaacgca ttatcatgac acaaggttcg gttaggtttt tccactgtaa
     5941 aacaaatttt tgaatttcct tttttaaatt atcaataaaa ctaacttcat tgtgctcact
     6001 tcctattttt gcaaataaca atgtttcatt tgcatctgac aatcttacta ctgaaggttt
     6061 taagtcattt tgtgtgagtt cgtttaggta atttgcacca gcttcaaaac ttggaaataa
     6121 tgcagtcagc caacgacttg attttggtag tttttttatt tttactttca cttcagaaac
     6181 aacaccaaaa acaccttcac ttcctacaaa taattgtaat aggtttaccc cagaagcatc
     6241 gtgtgtataa tcgttagaat gcaaagtgcc aattggtgtt accactttta tttgttcaac
     6301 gatatcttca atttttccat aataagaaga ttcttgacca gcacttcttg ttactaccca
     6361 acctcctaaa gtagaatatt caaaagattg agggaaatgc cctaaagtaa atcctttctc
     6421 atttaatatt ttttctaaag ctggtcccaa tatcccagct tggaaagttg cagtcaaatg
     6481 tgcttcatca atatgaataa gtttgttata gtttaataaa ttgatggttg cctttaaatt
     6541 atttttagaa ttattttctt gcatggtcaa tgctccaaca acatttgttc cgcctccaac
     6601 tggaattaat tttacttgat ggtcattggc ttgttttaca atatattcta tatcttcatt
     6661 tgtagtagga gctaaaacga catcgggcgt aacgattttg tcatctttac aaaggcgaat
     6721 gacatcgtaa taacttttgc ctaaggcaac acgaattctt tcctcatctc taaaagatag
     6781 tttttttgat gatagacttg caaaaacatc ttttaaggta tccttttttt tctcagtgag
     6841 tggttcaatt gaaaatttat taggcggaga atattcatgt ttgaaattcg ttttccataa
     6901 atcttctaaa aatgcttttg cttttggata atcattcatg tgttttaatt ccgcagggtc
     6961 gccccatttc caccaatctt tattcatgtt gtaaatgctt taaatattta gactaagata
     7021 caaaaaggca ctactatatt aaaaaagaaa atgttaaagt ttatagctta aagacataag
     7081 aaacgaaata attctttttt ggttaattta tgttatagaa tttaattgtc ttgaaaaata
     7141 taaaatcaat cactttaaag tgccaagaat tattattttt gatgtttaaa ttgaattaaa
     7201 ttatatttta tgaataaatt tcacttggtt tggattttag gtttgttgtt tacagcttgt
     7261 tctccaaaaa ctactgcaca gaaaagtacc tcaaatgcaa ctgaaaccaa taaagaagcg
     7321 actacatcac caatcaatgc aaataatact aatgaagatg taagtaatga aaatttagcc
     7381 aagtttaaac taccactaga caaaactgta cgttatggtg ttttacctaa tgggttgaaa
     7441 tattatatca aagctaataa taaaccagaa aatagagttg agttaagatt ggctgttaat
     7501 gctggttcaa atcaagaaga tgacaaccaa ttaggattag cacatttttt agaacacatg
     7561 gcttttaatg gaagtacaaa ttttaagaaa aatgatttag tgaatttctt agaaggcttg
     7621 ggtgttaagt ttggagcaca tttgaatgct tatacatctt ttgatgaaac ggtgtatatg
     7681 ttgcaattac cgacagataa aaaggaagtt ttagataaag gattgttggt attagaagat
     7741 tgggcatctg gaataagttt tgacagtttg gaaattaata aagaaagagg cgttgttgtt
     7801 tcagagtgga gaagtggtca aggtcctgat atgagaatgt taaacaaatt tttacctact
     7861 tattattata aatcaagata tgcggaaaga ttaccaattg gagatactac aattttgaaa
     7921 aactgtgctc acgaaagaat taccactttc tataaagatt ggtatagacc agatttgatg
     7981 gcagttatga tagttggtag tattaatgtg gattcaatgg aaaaagaagt tattgctcgt
     8041 ttttctgatt taaaaaatcc atcgattgaa agacaaaaaa tagaatatga gttaccaaaa
     8101 cacaaagaaa cattagtcag tatagtaact gatgatgaag ccagtaatac acaagcatat
     8161 atcgtttata agcacgataa acaagatatt tcaactactg aaggttttgt gatgcatgtt
     8221 gcaaatgaat taatcaattc tatgattaat aatagattag gcgaattggt agagcaaagt
     8281 tcttccccat ttattcaagc aggtgccgct tacgacaaag aaactagaaa aaatgatgct
     8341 ttttctgatt ttatatttat gaaaaaagat agatataaag atgctttgga gcttatttta
     8401 caagagaata atagagtaaa acaacatggg tttacagcct ctgaattggc gagagccaaa
     8461 actgaattgc taaaaagtta taatgatgca ttgaaagaaa aagataaaat ggagtctcgt
     8521 actttgatag gagaattggt aagtaatttc ttgaacaata caccagcatt gggaattgaa
     8581 gttgaaagtc aattggcttc acaaattatt tcagtgattg atttgaatac gattaatgag
     8641 attgctaaaa gttatattac aacagacaat agggtagtcg ttattactgc accaagtgct
     8701 gaaaaaagca agttgccaac tgaaaaagaa gtcttagcaa ttgttacaaa agcagataat
     8761 gaaaaattag aaccatacaa agatgaagta atagacgaac cacttatagg aactgaaatt
     8821 aaagctggaa aaattgtaaa tcaaacaact gataaaactt ttaatatcac aacctataaa
     8881 ttgagtaatg gagcaactgt aaaaattaaa ccgaccgatt ttaagaatga tgaaatattt
     8941 ttctctgcat ttagtaatgg tggaacatca ttatac
//
LOCUS       DAOQCW010000108         8825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_3042_length_8825_cov_4.799316, whole genome shotgun sequence.
ACCESSION   DAOQCW010000108 DAOQCW010000000
VERSION     DAOQCW010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8825
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3042_length_8825_cov_4.799316"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="PLS16_09355"
     CDS             <1..798
                     /locus_tag="PLS16_09355"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNJ61328.1"
                     /translation="DYDIFYTKFAGHAIKIAQQSLIEKYDIIAVVGGDGTQNEVGQVL
                     LNQPIILASIPTGSGNANARKMQIPIDVEKAIQLINTGKSFKIDACILNGQPFFMTAG
                     IGYDAKVVEQFDKIPFRGVGAYLSGVLSTAFTYDLANFHIEIDEHQIIDTKAFTVLIT
                     STGQLGYNVEFSHESVINDGIFEIAIIKDFNRARVPQLIYEAFWGDIAQQDFIERYPV
                     TKSVKIRTNKIESIQMDGDYKGKTNYIEVTCKKQALNFLVASDFSLA"
     gene            812..1159
                     /locus_tag="PLS16_09360"
     CDS             812..1159
                     /locus_tag="PLS16_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HNJ61329.1"
                     /translation="MSKQQQNWKDRLGTVYSTNPDFNYQTEDDDEVETLPNDKQILHI
                     QKDNKLRKGKIVTIVSNFIGKTEDLETLAKLLKTKCGVGGSSKDGEIIIQGDLRTKIK
                     EILEKESFKTKLR"
     gene            complement(1169..2731)
                     /locus_tag="PLS16_09365"
     CDS             complement(1169..2731)
                     /locus_tag="PLS16_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61330.1"
                     /translation="MEIKNYQLWLKILIGISILCGIVLFVQTTVDDSFICFRYGYNLV
                     KYSFWNWNPDSDRIEAYTSFTYMMLSVIPPLLNVQPQFVFKIITFIFFLLIIRRIYLL
                     TNNKFYALLVILALTANWQTHVHIYAGLETIFWFYLLLEVFGILYKEDLGPKAQTKLW
                     LLALLLPLTRPEGAVFSLFIFIYIFWIKKQKIHWSSLFFFASIGGIYFLWRFWYFGLP
                     LPLSFYHKSVGNNLGWLGLIFNTIMAWQYLLLGGFVWYLVRKSTLAKSFFIIVFFLYF
                     VFYGTTALLMNYANRFAFQLFYPAIIFGLIVIVNHLSSKERKRVMIAMFIFLGAIFYK
                     GLFDRMPMQFARIKDNMLFTAYMQKTHFTIGNQLAKLNNPKIKVAIGDAGVIPYLANK
                     KCYDPYGLADVQLSMHHLDEAYFNKMDADLLILSGSFRNEKMLSKDITNIGIMYNIIK
                     KQDTTYTLINKFNTDKGRYNLFCYIKNNSPYKAAIQEAMNKAQVQHDNFKITADDFLH
                     FRYMNNYFMKVE"
     gene            complement(2721..4379)
                     /locus_tag="PLS16_09370"
     CDS             complement(2721..4379)
                     /locus_tag="PLS16_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61331.1"
                     /translation="MKFTKQSFVDLLYIPISLVFLYIMFRNYTQFLDISMVDETGYME
                     LLNFNPLLYPGGYGPMYSLSYKIVYQFIPDFINLHYFFVIVLTWLPPLSLFFFLRSRK
                     VSFVISMLFSWLFFITSYIAAFDWWARTSHYSITLLFLFLIFINRYRQYPLLILAYTF
                     LFFKILSYVRPELNMASYFVLLFLIIIVIYKLVKRKYSKINLKLLDKLLLSSLVVVYL
                     SMFLMWKSPTTNTGRMYFALGQHYTMNYNKWNGLGRIDFLYWEKIFKEHFGESKTFSD
                     MYKNNPKETIKHVTENIKSYIIQTLDFVSELFLPKVYFKFNYIIKWIIIISVIVFFIF
                     RIGWNVYSKILIDKVIKDFYFLFVVTSFALPSLVASFVIFPREHYFIMQLPFFIYLFY
                     LILYPLVKIYNNVLHNKIFTIIVLVILFIGTPHVSKFVRYNNFQTYEYPNYLPYIKAV
                     RKLHIKKTINYLHHEINLIYVTKNFKKFGYFSGFPFYDSIIVKDSINMLYVNNFLFDL
                     KPHQSDSTFSYFMNNYPKLGWQKLQLEGKRGYIIYKKELLENGN"
     gene            complement(4449..5942)
                     /locus_tag="PLS16_09375"
     CDS             complement(4449..5942)
                     /locus_tag="PLS16_09375"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HNJ61332.1"
                     /translation="MILTVMGYIDMFQEKQAIRRIYPLVGRLRYVFEELRPKMYQYFI
                     ESDIDGRPINRVDRSTIYQRSKKTLQTIPFGTQLDVYAEGYEWMCHSMAPKAFDTLNH
                     NPRVVFGNKDCKQPYSASILNISAMSYGSLSDRAIEALNGGAKIGNFAHNTGEGSISN
                     HHLKQGGDLIWQIGTGYFGCRNEDGTFNPELFAEKANLQNVKMIELKISQGAKPGHGG
                     ILPASKNTEEIARIRHLKPHTVVESPPFHSAFSTPLEMIQFIQQLRDLSGGKPVGFKL
                     CIGHKSEFISICLAMIHLDIYPDFITVDGGEGGTGAAPQEFSNYIGAPMIDGLAFVHN
                     ILNGLDIRKYIKLIASGKITSGFHIARAIALGADTCNSARAMMMALGCIQALLCNTNR
                     CPTGIATQDPKLTVGLVVDDKKVRVANYHEETIKSFVEMLGASGLDEAKNISRSHIYR
                     RVYLNKMLTYEEIFPSVVLGSMLDGKIPEKYQTDFANANMHSWGIPS"
     gene            complement(6377..>6985)
                     /locus_tag="PLS16_09380"
     CDS             complement(6377..>6985)
                     /locus_tag="PLS16_09380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNJ61333.1"
                     /translation="QILFVRGNTPTTVLPIELLSFTGYNDGDVNVLNWVTASERNSAK
                     FEIEKSYDASTFEYIGEVPAAGNSIIELGYNFNDPNPQFGDNYYRLKMIDSDGTFKYS
                     EIINIKVREDNMAVQDGIIKIYPNPTNGYLNIQYQSSRDNNIDLQIFDMLGKNMLSKT
                     EHVTRGMNIIQVNAFSFAKAMYVLKLIDQFNGNEHQAKFIKE"
     assembly_gap    6986..7085
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7086..>8825)
                     /locus_tag="PLS16_09385"
     CDS             complement(<7086..>8825)
                     /locus_tag="PLS16_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61334.1"
                     /translation="GTYNISITNNGGSDAINGYIGLFRQNDDGNPVCPSTSWNAMSKV
                     KETSSAKVANDVTLNLECWEIEEGMTYYFQVQGIDGSLGGDVGNNFNVSVQFTAGTTN
                     PADNICTAPTANIGTTYSADNRCATTQPNEPNLDPNPQTPLDGKSYDETLWYKFVAPA
                     SGEVKLTLSNFSIPMLLSVNMQLYEAPTGYNCSVNHFNGLIPNESASLITSISPSIDF
                     RCLAPGKTYYIQFDGNDAFTDRGTWDFVITDLYSANVPPANDEPCGAIDVTQHIRSAA
                     LGPCSDDGQYYTETYSIADLAIDIDQATKTATAIGCNGELNCNDYWFKFTVPLDASGI
                     RIQGNDEYAATPGFNNSDEHIGIYRAPSGCGGALQQINCGSGGFNNDVDMTVAAYPGE
                     TLYLQVFDANAPANPSRTTFGFCLSVDCKAKLPCNSTADIGYDQPQCWNLNTNGVDHS
                     PIYYDCMSETNNAVNYFTFRTDCDGTPNDTVQVIFSLTEMGCGKTAMSIVSDNTPCTP
                     DFQDILLNCAVFQEVLGGTTATNFNQTYILPGCTNYIVQIIGDNNTASCASSGQILFV
                     RGNTPTTVLPIEL"
BASE COUNT         3239 a         1444 c         1164 g         2878 t
ORIGIN      
        1 gattatgata ttttctatac caaatttgct ggacatgcta ttaaaattgc tcaacaatcc
       61 ttaattgaaa aatatgatat tattgcagtt gttggtggcg atggcactca aaacgaagtt
      121 ggtcaagtat tattaaacca acccataata ttagcgagta ttcctactgg ttctggcaat
      181 gctaatgcaa gaaaaatgca aattccaatt gatgttgaaa aagccattca acttatcaat
      241 acgggcaaat catttaaaat tgatgcttgt attctaaatg gacaaccttt ttttatgact
      301 gctggaattg gatacgatgc taaagttgtt gaacagtttg ataaaattcc atttagaggt
      361 gtgggtgcat atttaagtgg cgttttgagt acagcattta cttatgactt agccaatttt
      421 catattgaaa tagatgaaca ccaaatcatc gatacgaaag cttttactgt tttaataact
      481 tcaacaggac aattaggata caacgttgaa tttagccacg aaagtgttat taatgatggc
      541 atttttgaaa ttgcaatcat caaagatttt aatcgtgcta gagttcctca attaatttat
      601 gaagcatttt ggggagatat cgcacaacaa gattttattg aacgttatcc agttacaaaa
      661 tctgtaaaaa tcagaactaa taaaattgaa agcattcaaa tggatggaga ttataaagga
      721 aaaactaact atatagaagt aacttgcaaa aaacaagctt taaacttctt agtagcttct
      781 gatttttcat tagcttaaaa aataaattat tatgagtaag caacaacaaa attggaaaga
      841 tagactgggc actgtttatt ctacaaatcc tgattttaat tatcaaactg aagacgatga
      901 tgaagtagaa accttgccta atgataaaca aatattacat atccaaaaag ataataaatt
      961 acgtaaagga aaaattgtaa ctattgtttc caactttatt ggaaagacag aagatttaga
     1021 aacgcttgcc aaactattaa aaacaaagtg tggtgttggt ggttcttcaa aagatggcga
     1081 aattattatt cagggagatt taagaaccaa gataaaagag atactagaaa aagaaagttt
     1141 taaaactaag ttgcgttaaa aatccaaact actccacttt cataaagtaa ttattcatat
     1201 atctaaagtg tagaaaatca tctgctgtta ttttaaaatt atcgtgttgc acttgagctt
     1261 tattcatggc ttcttgaata gcggctttat aaggcgaatt atttttgata taacaaaata
     1321 gattatatcg acctttatct gtattaaact tattgattaa ggtataagtc gtatcctgct
     1381 ttttgatgat attatacata attccaatat ttgtaatatc ctttgatagc attttttcat
     1441 ttctgaaact tcctgaaagt atcaacaagt ctgcatccat tttattaaaa taagcttcat
     1501 ctaaatgatg catagacaat tgaacatctg ccaaaccata aggatcataa cattttttat
     1561 tggcaagata gggaattaca cctgcatcac caattgcaac ttttattttg ggattattca
     1621 atttggctaa ttgattgcct atggtaaagt gtgttttttg catataggct gtaaacaaca
     1681 tattgtcctt aattctagca aattgcatag gcattctatc aaaaagtcct ttataaaaaa
     1741 ttgctccaag aaaaataaac atagctatca ttactcgttt tctttcttta gatgataaat
     1801 gattgacaat tacaatcagt ccaaaaatta tagcaggata aaacaactga aaagcaaaac
     1861 gatttgcata gttcatcaat agtgcagttg ttccataaaa cacaaaataa aggaaaaata
     1921 caataataaa aaatgatttt gctaaggtgc tttttctaac caaataccaa acaaatccac
     1981 ccaataataa atattgccaa gccataattg tattgaaaat caaacctaac caacccaaat
     2041 tattcccaac agatttatga taaaatgaaa gtggcaatgg taacccaaaa taccaaaaac
     2101 gccataaaaa ataaatacca cctattgatg caaaaaagaa caaagacgac caatgtattt
     2161 tttgtttttt aatccaaaaa atatagataa aaataaacaa cgaaaataca gcaccttctg
     2221 gtcgggtaag tggtaaaagc aaagctaaca accataattt tgtttgtgct ttaggaccta
     2281 aatcttcttt atacaaaatt ccaaatactt ctaataataa gtaaaaccaa aaaatagttt
     2341 ctaatccagc atagatatgt acatgtgtct gccaattagc tgttaaagct aagattacca
     2401 acaaagcata gaatttatta ttcgtcaata aataaatacg tctaataatc aataagaaaa
     2461 atatgaaagt aataatttta aaaacgaact gtggttgtac attcaacaat ggaggaatca
     2521 cagacagcat catatatgta aaactagtat aagcttctat cctatcacta tcaggattcc
     2581 aattccaaaa actatatttt actaaattat aaccatatct aaaacaaata aaagaatcat
     2641 caacagtagt ttgtacaaat aaaacaatac cacaaagaat tgaaattcca atcaatattt
     2701 taagccacaa ttgataattt ttaatttcca ttttctaata attctttttt gtaaataata
     2761 tatccacgtt ttccttctaa ttgtaatttt tgccaaccta attttggata attattcata
     2821 aaatagctaa aagtagaatc tgattgatgg ggttttaaat caaaaagaaa gttatttacg
     2881 taaagcatat taatactatc tttaacaatg atagaatcat agaaaggaaa tccagaaaaa
     2941 tatccaaatt ttttaaagtt ttttgttaca taaataagat ttatttcatg atgtaaataa
     3001 tttattgtct tttttatatg aagtttccta actgccttaa tataaggcaa ataattagga
     3061 tattcatacg tttgaaaatt attatatctt acaaatttgg acacatgagg agtacctata
     3121 aataatatta caagtacaat tatcgtgaaa attttattat gcaaaacatt attatatatt
     3181 ttaactaatg gatataatat aagataaaat aaatatataa aaaaaggtaa ttgcattata
     3241 aaataatgct ctcgtgggaa aattacaaaa gaagctacaa gtgaaggcaa cgcaaaactg
     3301 gtaactacaa acaaaaaata aaaatctttt ataactttat caataagtat tttactataa
     3361 acattccatc caattctaaa aataaaaaat acaattacac ttattataat tatccactta
     3421 ataatataat taaatttaaa atataccttg ggtagaaata attctgaaac aaaatccaaa
     3481 gtctgaataa tataactctt tatattttct gttacatgtt taattgtttc tttaggatta
     3541 tttttataca tatcggaaaa agttttactt tctccaaaat gttctttgaa tattttttcc
     3601 caatacaaaa aatcaattcg acctaaacca ttccatttat tataattcat tgtataatgt
     3661 tgcccaagag caaaatacat cctacctgta ttagttgttg gagatttcca cattaaaaac
     3721 atacttaaat acacaacaac taaagaggaa agcaataact tatccaaaag ttttaaatta
     3781 attttagaat attttctctt aactagttta tatattacta taataattaa aaatagtaaa
     3841 acaaaataag aagccatatt caactcagga cgaacataac ttagaatttt aaaaaataaa
     3901 aaggtataag ctaaaattag caatggatat tgtctatatc tattaataaa aataagaaat
     3961 aaaaataaca aagtaatgct ataatgtgaa gttcttgccc accaatcgaa tgctgcaatg
     4021 taggaagtaa taaaaaacaa ccaactgaat aacattgata taacaaaact tactttccta
     4081 cttcttaaaa aaaagaagag tgataatgga ggtaaccatg taagtactat aacaaaaaaa
     4141 taatgtaaat taatgaaatc tgggataaat tgataaacta tcttataact caaactatac
     4201 atcgggccat agccacctgg ataaagcaat gggttaaaat tcaacaattc catatagccg
     4261 gtttcatcta ccatagaaat gtctaaaaat tgagtataat ttctaaacat tatatataga
     4321 aaaactaaag aaattgggat atacaataaa tctacaaaag attgtttggt gaacttcata
     4381 ccttataaat ctgaaaagtg taaagatacc atttattaag tattattcaa ttttatgttg
     4441 tttaattttc aacttggaat cccccaacta tgcatatttg cgtttgcaaa atccgtttga
     4501 tatttttcag gaattttccc atctaacata gaacctaaaa caacagatgg gaaaatttct
     4561 tcataagtca gcatcttatt caaataaact cggcgataaa tatgacttct tgaaatattt
     4621 ttagcttcat ctaatcctga tgcacccaac atttcaacaa aacttttgat agtttcttca
     4681 tgataattag caacacgcac ttttttatca tcaaccacca aaccaaccgt caattttgga
     4741 tcttgtgtag caatacctgt tgggcatcga tttgtattac ataataacgc ttgaatacaa
     4801 cctaatgcca tcatcattgc acgtgcagaa ttgcaagtat ctgcacccaa agcaatcgct
     4861 ctcgcaatat gaaacccaga agtaattttt ccagaagcaa ttagctttat atattttcga
     4921 atatctaaac catttagaat attatgtaca aaagccaatc catcaatcat tggtgctcca
     4981 atataatttg aaaactcttg aggtgctgct cctgttccgc cttctccgcc atcaaccgta
     5041 ataaaatctg gatagatatc taaatgaatc atcgccaaac aaatagatat aaactcactt
     5101 ttatgaccta tacacaattt aaaaccaaca ggttttcctc cagataaatc tctcaattgt
     5161 tgaataaatt gtatcatttc taatggtgta ctaaacgccg aatgaaatgg aggcgactca
     5221 acgactgtat gtggttttag atgtcgtatc cttgcaatct cttccgtatt tttggaggca
     5281 ggtaaaattc cgccgtgtcc aggctttgca ccttgagaaa ttttaagttc aatcatcttt
     5341 acattttgta aatttgcttt ttctgcaaac aattcaggat taaaagtccc atcttcattt
     5401 ctacaaccaa aatatccagt accgatttgc caaattaaat ctccaccttg ttttaaatga
     5461 tgattgctga tactaccttc tcctgtattg tgtgcaaaat tacctatttt agcaccgcca
     5521 tttaaagctt caatagctct atcactcaag gaaccataac tcattgctga aatatttaaa
     5581 atacttgcag aatatggttg cttacaatct ttattgccaa aaacaacacg tggattatga
     5641 tttaaggtat caaatgcttt aggagccatt gaatgacaca tccactcata accttcagca
     5701 taaacatcta attgcgttcc aaatggaatc gtctgtaatg ttttcttaga acgctgataa
     5761 attgtagaac gatctactcg attaattggt cgaccatcaa tatctgattc aataaaatat
     5821 tgatacattt tgggtcttaa ttcttcaaaa acatatcgca atctaccaac taatggatat
     5881 attctacgaa tagcttgttt ttcttgaaac atatcaatat aacccataac tgtcagtatc
     5941 aaaaaaattg aaaataatac aaaccaattt ttatcgaaga atatagagag ggctaaagaa
     6001 atacacaaca agaaacttga aatgataata aacttgagac gcatagcaaa tacattttaa
     6061 gttaaaaaaa tgctacaaga ccgttgcaag ttaattatta ttcataaatc tcaagccaaa
     6121 ttctttcaat catcttcgtt cctaatttat ttttaatcct cattatcaat ggataactgc
     6181 ggtataaaaa taaatgagtg ccttgaatat ttttgtaatt tgacttttac aacggtcttg
     6241 attcttaaaa gtaaatattt ttgtacgaat taccaagtta tttaactttc taaagtatct
     6301 cacacaaaat ccataaaata gaaacagtca cttaaaagtg actgtttcca atttctaaat
     6361 tagtttaaga tatctgctat tctttaatga atttagcttg atgttcatta ccattgaatt
     6421 gatcaatcaa ttttaaaaca tacatcgcct tagcaaaact aaatgcattt acttgaataa
     6481 tattcatacc tcttgttaca tgttcagttt tactcaacat atttttacca agcatatcaa
     6541 aaatctgcaa atctatatta ttgtctcgac tagattgata ctggatattc aaataaccat
     6601 tagttgggtt aggatatatt tttataatac cgtcttgaac tgccatatta tcttcacgaa
     6661 ctttaatatt aataatttct gaatacttaa atgtaccatc actatcaatc attttcaaac
     6721 gataataatt gtcgccaaac tgaggatttg gatcattaaa attatagccc aattcaataa
     6781 tactatttcc tgcagctggc acttcaccaa tatattcaaa agtagaagca tcataagatt
     6841 tttcaatttc aaacttcgct gaatttcttt ctgaggcagt tacccaattt aagacattca
     6901 catcaccatc attgtatcca gtgaaagata ataattcaat cggtaataca gttgttggag
     6961 tatttcctct aacaaataat atttgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnataat tcaatcggta atacagttgt tggagtattt cctctaacaa ataatatttg
     7141 acctgatgaa gcacaagaag ctgtgttatt atcaccaata atctgaacga tataattcgt
     7201 acatcctggt aaaatatagg tttggttaaa gtttgtagca gttgttcctc ctaatacttc
     7261 ttggaataca gcacagttta gtaatatatc ttgaaaatct ggtgtacaag gtgtattgtc
     7321 agaaacaata gacatcgcag ttttaccaca acccatttcc gttaatgaga aaatcacttg
     7381 aacagtgtca tttggtgtac catcacaatc tgttctgaat gtaaaataat ttacagcatt
     7441 attagtttca gacatacaat catagtatat aggcgagtga tctacaccat ttgtatttaa
     7501 gttccaacat tgaggttgat cataaccaat atcagctgta gaattacatg gaagttttgc
     7561 tttacaatct acagacaaac agaatccaaa ggtagttcta ctaggattag caggggcatt
     7621 agcatcaaaa acttgcaaat ataaagtttc tcctggatag gctgcaacgg tcatatctac
     7681 atcattatta aaaccaccag atccacaatt gatttgttgt aatgctcctc cacaaccact
     7741 cggtgctcta tatattccaa tatgttcatc agagttatta aacccaggcg ttgctgcata
     7801 ctcatcattc ccttgaattc taattccact tgcatctaat ggaacagtaa atttaaacca
     7861 atagtcgtta caattcaact ctccattaca acctattgca gttgcagttt tagttgcctg
     7921 atctatatcg attgctaaat ctgcaatact atatgtttct gtatagtatt gtccatcatc
     7981 ggaacaaggt cctaatgcag cacttcttat atgttgagta acatcaatag caccacatgg
     8041 ttcatcattt gctggtggta catttgctga atacaaatct gtaataacaa aatcccaagt
     8101 acctctatct gtaaatgcat cattaccatc aaactgaata taatatgttt ttcctggtgc
     8161 taaacatcta aagtctattg atggtgaaat actagttatt aagctagcag attcatttgg
     8221 aatcaaacca ttaaaatgat taacgctaca attatatcca gttggtgctt catacaattg
     8281 catgtttacg gatagtaaca taggaatact aaaattagac aatgtcaatt taacttcacc
     8341 agatgctgga gctacaaatt tataccataa agtttcatca taagattttc catctaatgg
     8401 tgtttgtgga tttggatcta aatttggttc attaggctgt gtcgttgcac aacgattgtc
     8461 cgctgaataa gttgttccaa tatttgctgt tggtgcagta cagatattat ctgcaggatt
     8521 cgttgttcca gcagtaaatt gaactgaaac attaaaatta ttacccacat cgccacctaa
     8581 tgaaccatct ataccttgca cttggaaata ataagtcatg ccttcttcaa tctcccaaca
     8641 ttctaaattt aaggtaacat cattagcaac ttttgcagaa gaagtttctt tcactttgga
     8701 catagcattc catgatgtac ttggacaaac tggatttcca tcatcgtttt gtctaaataa
     8761 tccaatataa ccatttatag catctgaacc tccattattg gttatagaaa tattgtaagt
     8821 tccat
//
LOCUS       DAOQCW010000109         8562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_3199_length_8562_cov_5.272952, whole genome shotgun sequence.
ACCESSION   DAOQCW010000109 DAOQCW010000000
VERSION     DAOQCW010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8562
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3199_length_8562_cov_5.272952"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1227)
                     /locus_tag="PLS16_09390"
     CDS             complement(<1..1227)
                     /locus_tag="PLS16_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61335.1"
                     /translation="MSVHTQGALPIQIFKDRRPLESENKQAFNFRSNNYRAITKNEFD
                     KAIADYTTTALNLDAVVNYGTNDDLKAYEDTLKLNNTYTSFSLKEWIIKRVGWYKQTD
                     PNAVVVVLPKHISETFVPNYLFDLPDFNNIINQKIDIDIRLIGYADIVDISNDYLVFK
                     GGDYQVNDSKSYPYYFGVDKELTVLFIPKITDNKLSYESHIYYNNNLTNAPFYVIGSR
                     YVLDADGSEYFISDYHGAAGWGDLAIGQGSDLRICEIRFVYPRHWRVKVPCDNVMEGG
                     CHLDTSLNRHVTSDGHTCGRCSGTGYIMDTTPTGTLMVSKGGDFLDEQGKFTVPEGFI
                     TPDTSILQHSANREAYYFDMMLNALCVSKQNMTNQSGESKRYDSQQRVDLVSGILFDL
                     FKLYENILNSIAEYRGG"
     gene            complement(1327..1920)
                     /locus_tag="PLS16_09395"
     CDS             complement(1327..1920)
                     /locus_tag="PLS16_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61336.1"
                     /translation="MAICTPNCSEAQIRSYPVPCKRAESLLKGGSEFFILLDCDIEFT
                     DITSAVEWEGYITANKVIVSPPGLGNIIKPTTSKLILSAGKPEEVISEISGYDFKTSV
                     LDPTTFKHIDFLNDVKNLAHSKQLMWVECDGNIYKKTAKEGFDGLAVEGWRVSEDGSK
                     QSINFEFRFETYGVEIKPTPLTDAIRDVLFAATSSSN"
     gene            complement(1933..2148)
                     /locus_tag="PLS16_09400"
     CDS             complement(1933..2148)
                     /locus_tag="PLS16_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61337.1"
                     /translation="MTTYDFIKTLEKNKIYTVIIEGQTIKKTGDELRSYLITHSIESI
                     DYAEEVKQTEHKVTAPTKKIDTTIDNK"
     gene            complement(2578..4197)
                     /locus_tag="PLS16_09405"
     CDS             complement(2578..4197)
                     /locus_tag="PLS16_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61338.1"
                     /translation="MLEFPIYQPLQFNRGCDEILVRKDSTINAVFNSKWEIKAVDDFN
                     DITTLPTHGNVLNAGETLFLSFITSNYKNSYTCGVDTNSRIYLYTFKKLIIFSVVPTV
                     IDDVVNEYDVVYDTEDMTIVNISKNSGLEFVYGISNYLKSQFIIDSDFTIVASGSNLE
                     ITFLTAKLGYTTIEVLGSSPASATPVLHWGFRTYWNRRLRSLVAWNEGKHLDSCGWGG
                     KTDATFYVRNWQSELINEHKKSCRIRFKIRSMIEDVAFDYKFKLVTSGGDILAEQTVN
                     VPANIELEYQNIFILPATPFNLDNLQLISGDSKPAGSEWSGKDDLFYIKDVEIATKDD
                     IAKIDLYNCEGVKIVDDYNATINQNEDETTSVIELPYNSNMASVVITSVQGDIYQSNA
                     IKYIDYNANDKCFKDLLPLKWSNSCRFANTDYGNLEIEHEIFIRAFLMYQELRDLETE
                     FITTHAGKEKMLFYVSNENYELRIANYTQVIHSIVQYALLHNILYLGGEKYFRNQNSK
                     YTLAEYNNGYFTAKIQLSKDDSTLIVRNGCC"
     gene            complement(4191..6050)
                     /locus_tag="PLS16_09410"
     CDS             complement(4191..6050)
                     /locus_tag="PLS16_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61339.1"
                     /translation="MQEDIIKYYIDNKHIEDISGDVSATIIREDGFANSEQILREKTD
                     SQLTAYLCGYDFLAQKRKEDYCRDLPLRIELNNKELFRGTIRQNDLKVQVTKGIIKVD
                     NIKDESFSNMIAQFKDVEVPLNSTMTRSGEPINAIPIQRPLMLIVLRSYLVLNVLDVI
                     RYLVDFITDNEVEVISRYLSNANIFISTGYAMRHAKGTAEQMFPTLSLSQVLEEVRKA
                     TSLYGVMTEVAGKPTLILEKEEDTFGSDVLINIDTLPLGLDESFDDNQIFNSIQLGGN
                     RKIEQSSERLENFNNIEVFSCGCNGFKDNVLNLRFNFIVDNDTIIRQLDSANYNDDKV
                     WNDDIFMFELDGLGIYNNNQRFKNINILNEWLGSGINCFTKDMLAKYGFLAINRDDFS
                     SYQLGFLYFKNPIESILDRQNSLHNWIYAIDGMPMIMTPTQDGTTYFKCQEAGNYTFN
                     SKVSVKGLSAGTYDASTNTILLKLMKASSISNAVAGIILETLTENLIDGYEIVVGEDF
                     QLEIQGSFNLAVNDVVIAMYEITRDPTCYITYQEFSLVRDSLTVLTSENSDHYKPFLA
                     KFKYDLCFEDYEKMRKNKTGIIRINGVDTWIKEVKFNPFGASDFTVKYKNTLC"
     gene            complement(6031..>8562)
                     /locus_tag="PLS16_09415"
     CDS             complement(6031..>8562)
                     /locus_tag="PLS16_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61340.1"
                     /translation="STGATSMRSGIRATREELSKMLVSGEANTAQLYKMAKAGGELSD
                     AMGDASQAISVMASDTFPLDSMLQGVQSITAGFQVAEGAMALFGVENEDLQKTLVKLN
                     ALMAITSGLQELQNSLQKTSALSLGFNVVMQKAYTLAVGESTGAMKLFRLALISTGIG
                     AFVVLLGVAVSAFMKWSESSKQASKNLKLLADVNKEATQNIEDERAELTKLVAIAKNE
                     NLSKEQRKNAIKQINENYPEMLGNITLENIGTEKANELLGKQIQILVAREKVKLLSSK
                     IAQAQIDLELAKNGELLSSYTKSRIAINRFFGATKDADRIKNEAITEKATEIENTITS
                     LTTELNKTLANLGNNVNEKTATNVETATSKVAKETKKKVEIAKSELEIYREELSKFEK
                     ELEELQFKRVRMPISKKEEDRIKDLTAQIQKMKDVLDSMTVKQSKPVVVVAKSELEIY
                     KDELSKLEKELEELQFKRVRMPISENEKKRIEDLTAQIQIMKDVLNSLTVQPSQPIGF
                     IQQLENSLTKTKEELEKLFNETISSGGAIDFDDTNVVELQDKIKSLTSDLEFAKETFN
                     QLMNGDTTTDDSSEGGGCGDDVDKKAERLKELTANMNSFAEKYQVIQSGISQATGLAS
                     ELFVRHTQKETQALDDKLKRGVISQKQYDKEIAKLKNEQARKEKRAKTAEAFAMIPMA
                     ILSTFTNTTGGLIIKNIAAGIAGAFALATALMVATAPLPKYRHGGQVFKGNGFVKGRS
                     HSEGGVNAELEGNEFVMKREAVAMYGVNNFNKLNNGLLRPDIFSPNVSNYTNQLERID
                     YTKEFKKMNEILEFIYQSTEDGNNIAMQVGKKLIAKENKDARRYN"
BASE COUNT         2533 a         1765 c         1164 g         3100 t
ORIGIN      
        1 acctccacga tattccgcaa ttgaatttaa aatattttcg tataacttaa ataagtcaaa
       61 taaaatacca ctcaccaaat caactcgctg ttggctatca tatctcttac tctccccact
      121 ttgattagtc atattttgct ttgaaacgca tagagcattt aacatcatat caaaataata
      181 tgcttcacga tttgccgaat gttgaagtat tgaagtgtca ggcgtaataa atccttcagg
      241 tacggtaaat ttgccttgct cgtctaagaa gtcgccaccc ttagaaacca ttaatgtacc
      301 agttggggtt gtgtccataa tatagcctgt accactgcac cgcccacacg tatgaccatc
      361 actggtaacg tgtctattta agcttgtgtc taaatgacaa ccaccctcca taacgttgtc
      421 gcacggtact ttaaccctcc aatgacgagg atatacaaaa cgaatttcac atattctaag
      481 gtctgaacct tgaccgattg caaggtcgcc ccaacctgca gccccgtgat aatcagaaat
      541 aaaatactcc gagccatcgg catctaaaac ataccttgaa cctatcacat agaatggggc
      601 atttgtaaga ttgttattat agtaaatatg gctttcataa ctcaacttat tatctgttat
      661 ttttggaata aataatacag ttagttcttt atctacacca aaataataag gataagactt
      721 actatcatta acctgataat cgcctccttt aaatacaagg tagtcattgc taatatcaac
      781 aatatcagca taaccaatta acctaatatc tatgtctatc ttttgattaa taatattatt
      841 aaagtcaggt aaatcaaata aataattagg tacaaatgtt tctgaaatat gctttggcaa
      901 aacaacgacc accgcattag ggtctgtctg cttataccac cctacacgct ttataatcca
      961 ttcttttaaa ctaaatgacg tataggtatt atttagcttc aaagtgtcct cataggcttt
     1021 taaatcgtca tttgtgccat agttaacaac cgcgtctaaa ttaagggcag tcgttgtata
     1081 gtctgcaatt gctttgtcaa attcattctt agtgatagca cgataattat ttgagcgaaa
     1141 attaaaggct tgtttgtttt cgctctccag cggtcgccta tctttaaata tttgaatagg
     1201 caaagccccc tgcgtgtgta cgctcattat attataaacc tcctgccatt ttgttaaagc
     1261 aggaggtcta taattactta gtattttttt tgttatactt aaaatattca tacaacaaaa
     1321 ataatattaa ttagatgaac ttgtagcagc aaataataca tctctaattg catctgttaa
     1381 tggcgttggc tttatctcaa ctccgtaagt ttcaaatcta aattcaaaat taatagactg
     1441 cttagaacca tcttccgaca ctctccaacc ctcaactgct aatccgtcaa agccctcttt
     1501 agctgtcttt ttgtaaatgt tgccatcaca ttccacccac attaattgct tagaatgtgc
     1561 taaattctta acgtcattca agaaatcaat atgcttgaaa gtagtagggt ctaatacgct
     1621 tgttttaaag tcgtaaccgc ttatttctga tattacttct tcaggtttcc ctgctgataa
     1681 tatcagtttc gaagtcgttg gcttgattat attacctaac ccgggtggcg atactattac
     1741 tttatttgcc gtgatataac cttcccactc aacggcactt gttatatcgg taaattctat
     1801 gtcgcaatca agcaaaataa agaactccga accacctttt aaaagtgatt ccgctctctt
     1861 acatggcaca ggatagcttc ttatctgtgc ttctgaacaa tttggtgtgc aaatagccat
     1921 atctttttaa ttttatttgt tatcaatagt tgtatctatc tttttagtcg gagcagttac
     1981 cttatgctcc gtttgtttca cttcttcggc ataatcaatg ctttcaatcg aatgagtaat
     2041 taaatagctt cttaactcat cgcctgtttt ttttatcgtc tgcccctcga ttattacggt
     2101 gtaaattttg ttcttttcga gtgtctttat aaaatcataa gtagtcatat tctaattatt
     2161 tcttcaaaaa taaatagata tatttgctcc ttattggtaa attctgtact tttattccgt
     2221 ggacgattta aaagaatttg taaaagactt aaatataaag attatagcga tagataaata
     2281 tgtctatcgt gtccatattc tttattcaaa taacgatgct aaagcacttg acggcattat
     2341 tattttacaa gcacatttaa tcgttcttta taattttttc gataagttcg atgtggaggt
     2401 tatcatcgag aaaatgcgta aaataggcat taagaaagtc gagcaagggt atgttgatta
     2461 tacgtgcaat aacaatgaat acatacatct gctaatgata aagaatagtg ataaggtaaa
     2521 ggctaagttg ctttatatca taaatagaat tgattacttt ttactaacgt taaggcacta
     2581 gcaacaacca tttctaacaa ttaaagtact atcgtctttt gatagctgga tttttgccgt
     2641 aaaataacca ttgttatatt cggctaatgt atatttacta ttttgatttc taaagtactt
     2701 ttctccacct aagtatagga tattatgcaa taatgcatac tgtacaatac tatgtattac
     2761 ttgcgtataa tttgctatcc ttaactcata attctcattg ctaacataga ataacatctt
     2821 ttcttttcct gcgtgagtag ttataaattc agtttccaaa tcacgcaact cctgatacat
     2881 caaaaatgct cttataaaaa tttcgtgttc aatttctaaa ttaccataat cagtatttgc
     2941 aaacctgcaa ctattactcc acttcagggg taataagtct ttaaaacact tgtcatttgc
     3001 gttataatca atatacttga tagcattact ttgatagatg tcgccctgca cggaggttat
     3061 tacaacactt gccatatttg agttataggg cagttcaatt acacttgtag tttcatcttc
     3121 attttgattg atagttgcat tataatcatc aacaatcttc acgccctcac aattatacaa
     3181 gtctattttt gcaatatcat ctttagtagc tatctcaaca tctttgatat aaaataggtc
     3241 gtcttttcca ctccattcac tacctgctgg cttactatcg cctgatatta attgcaagtt
     3301 gtctaagtta aatggcgtgg ctggtagaat gaatatattt tgatattcaa gttcaatatt
     3361 agctggtaca tttaccgtct gctccgctaa tatatctcca ccactcgtta ccaacttaaa
     3421 tttataatca aatgcgacat cttctatcat tgagcgtatc ttgaaccgaa tacgacaact
     3481 ttttttatgc tcattaatta gttcactttg ccagtttctg acgtagaaag tagcatctgt
     3541 cttaccaccc caaccacaac tatctaaatg cttaccctca ttccacgcta ccaatgatcg
     3601 caatcgtcta ttccaataag ttctgaaacc ccaatgtagt actggtgtag cacttgcagg
     3661 actcgaacct aacacttcaa tagtcgtata gccaagtttt gcagtaagaa aagttatctc
     3721 taaatttgag ccacttgcca ctatcgtaaa gtcgctatca ataataaatt gcgactttaa
     3781 gtaatttgaa atgccataaa caaactctaa cccactattt ttagatatat ttacaatagt
     3841 catatcctct gtatcataaa caacatcgta ttcatttaca acatcatcaa taaccgtagg
     3901 cactacacta aatattatta gcttcttgaa tgtgtacaaa taaatccttg agttagtatc
     3961 aaccccacac gtataactat tcttataatt gctcgttatg aacgataaaa aaagcgtttc
     4021 tccagcattt agcacgtttc cgtgagttgg caaagttgtt atgtcattaa agtcatctac
     4081 tgcttttatt tcccatttag aattaaatac agcattaatt gtactatcct ttcgtacaag
     4141 tatttcatcg caacctctat taaattgcaa cggttgatat attggaaatt ctaacataat
     4201 gtatttttat attttactgt aaaatctgat gctccaaatg gattaaactt aacttcttta
     4261 atccaagtat caacaccgtt tattcttatt attcctgttt tattttttct cattttttca
     4321 taatcttcaa agcacaaatc gtacttaaat ttcgctaaga atggcttata atggtcgcta
     4381 ttctcactcg ttaataccgt taaactatca cgaacaagcg aaaattcctg atatgtaatg
     4441 tagcaagttg ggtcacgagt tatctcatac atcgcaatca caacatcatt gacggctaaa
     4501 ttaaacgagc cttgaatctc taactgaaaa tcctcgccaa ctactatctc ataaccatct
     4561 attaggttct ccgttagtgt ttccaaaata ataccagcaa cagcattgct aattgacgat
     4621 gctttcatta gcttcaacaa tatagtattc gtacttgcgt cataagtccc agctgataag
     4681 cctttaactg atactttaga attaaacgta taattacctg cttcttgaca tttaaaatat
     4741 gtagtgccat cttgcgtagg tgtcataatc atcggcatac catcaatagc gtatatccaa
     4801 ttatgtaggc tattttgcct atctaaaata ctttcgatag gatttttaaa atacaaaaag
     4861 ccaagttgat aactactaaa gtcatcacgg ttaatagcta agaagccata ttttgctaac
     4921 atatcttttg tgaagcagtt gataccacta cctagccact cgtttagtat atttatattt
     4981 ttaaatcttt gattattatt ataaatacct aacccgtcta actcgaacat aaatatatca
     5041 tcgttccaaa ccttatcatc attataatta gcactatcta actgccttat gattgtatca
     5101 ttatcaacaa taaaattaaa acgaagattt aaaacattat ctttgaagcc attgcaccca
     5161 caactaaaga cttctatgtt attaaagttt tcaagccttt cactactctg ctcaatcttt
     5221 ctattacccc ctaattgaat gctattaaat atttgattat catcaaagct ttcatctaaa
     5281 cctaatggca aagtatctat gtttattagc acatcgcttc caaatgtatc ttcttctttt
     5341 tctaatatta gcgttggctt tcctgcaacc tcggtcatta ctccgtatag gcttgtagcc
     5401 tttcgcactt cttcaagcac ttgactaagt gaaagtgtcg gaaacatttg ctccgccgtt
     5461 ccttttgcat gtcgcatagc ataaccagtg ctgataaata tatttgcatt acttaggtat
     5521 ctggaaataa cttcaacttc attatctgta atgaaatcta ccaaataacg tataacatct
     5581 aacacattta acactaaata tgaacgtaac acaataagca tcaaaggtct ttgaattggt
     5641 attgcattta taggctctcc actccgtgtc atcgttgaat ttaaaggaac ttcaacgtct
     5701 ttaaattgtg caatcatatt actaaagctt tcgtctttaa tattatcaac cttgattata
     5761 ccttttgtta cttgcacttt caaatcattc tgccttatcg ttcccctaaa taactcttta
     5821 ttatttaatt caattcttaa aggcaaatca cgacaataat cttcttttct tttttgtgct
     5881 agaaaatcat aaccacaaag ataagcggtt aattgactat ctgttttttc tcgtaaaatc
     5941 tgctcactat tcgcaaagcc atcttcacga atgatagtag cagatacatc tccacttata
     6001 tcttcaatat gcttattatc aatgtagtat ttaattatat cttcttgcat ctttattttc
     6061 tttggctatt aattttttac ccacttgcat cgcaatatta ttaccgtcct ctgtcgattg
     6121 gtaaataaat tctaatattt cattcatctt tttaaattct ttcgtataat caattcgttc
     6181 taattgatta gtgtaattgc taacatttgg agaaaatata tcaggtcgaa gtaagccatt
     6241 atttagctta ttaaagttat taacgccata cattgctact gcttcacgct tcatcacaaa
     6301 ttcgttgccc tctagttcgg cattaacacc gccctcacta tgtgaacgac cttttacaaa
     6361 gccattacct ttaaatactt gacctccgtg tctatatttc ggtagcggtg cagttgctac
     6421 cattaacgct gttgcaagtg caaacgctcc tgctattcca gccgctatat ttttaattat
     6481 cagcccccca gttgtatttg taaatgtgct taaaatagcc attggtatca tcgcaaatgc
     6541 ctccgcagtt tttgctcttt tttctttcct tgcttgttca ttttttaatt ttgcaatttc
     6601 tttatcatat tgcttttgtg atattacacc acgctttagt ttatcatcta aagcctgtgt
     6661 ttctttttgc gtgtgtcgta caaataattc agatgccaag ccagttgctt gagatatacc
     6721 actttgaatt acttgatact tttcagcaaa gctattcata tttgctgtta attcctttaa
     6781 cctttcggct tttttatcaa cgtcatctcc acatcctcca ccctcgctac tatcatctgt
     6841 tgtcgtatct ccgttcatta attggttaaa tgtttctttt gcaaactcta aatcgcttgt
     6901 taatgatttt attttatctt gaagttcaac tacattagta tcgtcaaagt caatagcacc
     6961 tccgcttgat attgtttcat taaatagctt ttcaagttct tcttttgttt ttgttaaaga
     7021 gttttcgagt tgctgaatga agccaatagg ctgtgatggc tgaacagtca agctattcaa
     7081 tacgtctttc attatttgaa tttgagcagt caaatcctct attcttttct tttcgttttc
     7141 tgaaattggc attctaaccc tcttgaactg taattcctct aattcttttt ctaatttact
     7201 taattcatcc ttgtatatct ccaattccga cttcgcaacc accactactg gtttagattg
     7261 tttgacagtc atactgtcaa gcacgtcttt cattttttga atttgagcag tcaaatcttt
     7321 aattctatcc tcttcttttt ttgatatagg cattctaacc ctcttgaact gtaattcctc
     7381 taattccttt tcaaatttac ttaattcttc cctatatatt tctaattcag atttagcaat
     7441 ctctactttc tttttagttt ccttagcaac cttagatgtt gcagtctcta cattggttgc
     7501 cgttttttca ttgacattat taccaagatt agcaagtgtt ttatttaatt cggtagttag
     7561 cgatgttatt gtattttcaa tttcagtagc tttctctgtg attgcttcat tttttattct
     7621 atcagcatct ttcgttgctc caaaaaaacg attaattgca attcttgatt ttgtataaga
     7681 gcttaaaagc tccccattct ttgcaagctc taaatcaatt tgtgcctgtg ctatttttga
     7741 cgataacaac tttacctttt ccctggcaac caaaatttga atttgctttc ctaacaactc
     7801 attagccttt tccgtgccga tattttcaag cgtgatattc cctaacattt cagggtaatt
     7861 ctcattgatt tgcttgatag cattcttgcg ttgctcctta cttaaattct cattcttagc
     7921 aatagcaact aatttagtta attccgctct ttcatcttcg atattttgtg tagcctcttt
     7981 atttacatct gccaacagct ttagattttt agacgcttgt tttgaacttt ccgaccactt
     8041 cataaaggca gaaactgcca ctcctaacaa taccacaaat gcccctatac cagttgatat
     8101 taacgctaac ctaaatagct tcatcgctcc agtcgattcc ccaactgcta aagtataagc
     8161 cttttgcata acaacattaa aacctaaaga cagtgcagat gttttttgca aactattttg
     8221 caactcctgt agtccgctgg ttatagccat taatgcgttc aattttacca acgtcttttg
     8281 caagtcttca ttctcaactc caaataatgc catagcaccc tccgccactt ggaaacctgc
     8341 tgttatagat tgaacacctt gcaacattga atctaaagga aatgtatcag atgccattac
     8401 agatatagcc tgacttgcat cgcccatagc atcggatagt tcaccccctg ctttagccat
     8461 tttataaagt tgagccgtat ttgcttcgcc actcactaac attttcgaaa gttcctcacg
     8521 tgtggctctt atgcctgacc gcatacttgt agcaccagta ga
//
LOCUS       DAOQCW010000110         8342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_3328_length_8342_cov_6.482442, whole genome shotgun sequence.
ACCESSION   DAOQCW010000110 DAOQCW010000000
VERSION     DAOQCW010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8342
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3328_length_8342_cov_6.482442"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4913..5074
                     /locus_tag="PLS16_09420"
     CDS             4913..5074
                     /locus_tag="PLS16_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61341.1"
                     /translation="MLSIVTSEVVAPMMNKIMYIIIVYNFWDQIAMCMILNMTGTAFI
                     IGKLIAVVS"
     gene            complement(8156..>8342)
                     /locus_tag="PLS16_09425"
     CDS             complement(8156..>8342)
                     /locus_tag="PLS16_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61342.1"
                     /translation="SKLFNNNPMNKIMMMKMMLQKTKKKKMETKKKETKKERMTKKER
                     MTKTKMLNKNKMLIKVT"
BASE COUNT         2762 a         1291 c         1348 g         2941 t
ORIGIN      
        1 taaaatccga aaaatcatat ataaaaaaag tggaccgaga tgtaatttca gaaaaaaaaa
       61 aaattactat taaaaaaaaa aagtcattaa ttttggaaaa taaaaaaagt tcatgctcat
      121 aaatttagat attttttaat taatttaatg acgaatgagc tatcatttgt gaggaagacc
      181 cttactttgg tttctttttg tatttcttgt tgaaatttta taggaacttt tggatttaga
      241 tttagatttg gtttttcttt ttctttggat cggaatcgat tcacttgatt tatcttcaag
      301 aaataggtaa ggcttttgtt tttcatccat cacttcgatg agtttatatc cttaaaggtc
      361 aaaatgatct ctgaattttt tctctccaaa catgtttttt tctctttaac acaaaacaaa
      421 agcattgtgg tctcggaaat cagggacatg ctctttttca agagattaga tatatttctt
      481 ctcaaattct tgttcttaaa aggttggttc tggttttggt ggttggctga ctaattgatt
      541 cataatttag ttttatcgct gattcttgac ccataagcca agtgagactt aatttctgaa
      601 atgctcattc tttttcattg gttcatatgt ttcggggtta aagaaacaat agaggtaggg
      661 attattgaga agcttattga tttatcttta ttataaatca atattctctc ttttcttaac
      721 aacataaagt tatcctttgg catttctttt gactctccga tattctttag ttttttcagt
      781 atctggcatg gatcttttga attagtcgat gatttagtca gatgttgctt tatatttcca
      841 cgaagcatca taataagaag gagaaccaat caaatgataa taataatagt acaaattttc
      901 tcccggataa tacaatttct cacctgtttt gatttttcgg acttttttct cgatgaatcc
      961 tttcagagtt tagtgatatt atggttttcc gtcaacaaca cacatatctt aagaaatcca
     1021 gccattctaa atagcctttt caatgtaatt ttagaaaata tcgtatcttt tccattcatc
     1081 ccattgatct taggcagaag ccaatattct ctgcagatat tttttaaaat atctcctcag
     1141 ccaattcttg tgaatattga agagacattt caaagcaata acgaacatac cgtgctaaaa
     1201 acacaagcaa gcgacatcaa aaggcatttc aagttcctta gtctaaattt attgcttgac
     1261 atctttttag attttgatga ttccagcacg actttgagtt gagtgttgga ttctcagttc
     1321 ttatttcaca tcataatttt ttattctgtt cgaggttgca ttgatagaga taagattcca
     1381 tttctgtttg agagttctta cagtcaaacc attaacaaat gatatatcat gttttttttg
     1441 gtagtgattt ttcatttgac ctttatgtcc attaaacgga caaaatatgc atttttagct
     1501 tcccttgttt ttattagagt gtgatttgtg atgagtgagt agattctaaa aactataaca
     1561 ggtgaaagaa caatccttca tactacaact tatttattct tcttatttag tcacttttcc
     1621 agttttaaat taaacatatt aaatttagct tttgaggggc ggattgatca gatgtgaatt
     1681 aatctttgaa gttgaggagg tgtattgact attttttgtt ttatttagaa ttgaaacaca
     1741 aatgggaatg tgcattgcaa gttccgattc atagtcaaat tttttagaac aattttggca
     1801 agtgagaaat tagatttagt agctttacaa gggctaacta attacttaag acacttaatt
     1861 aacaataggt tttttcttgg aaattttgca agttggggtg tgcagagcaa gtttgtattc
     1921 tgaatcatat ttttgagaac aattttggca agtcaagaat tatgcttaat aattcagaat
     1981 tgatggcatg aaccttttat aaggacattt ttagatatga ctaattgcct ggctttaaca
     2041 aaatcccttg aattagcagt aaggacagtc caaatctttt atcatgaaaa tattatacaa
     2101 caacacacac attttttcga ttcttaaatt gctaaacatc tctgcatgaa tgttcaatat
     2161 attattatta gtataagtct aataccttac ttccctgatt cttgagttat tatcacaacc
     2221 tttatttttg ttacaatggg attttttgaa gaatgtttat aaggcttttt tttttttttt
     2281 tttagataat caaaggaaaa gcatatttat aacttgttag cgtatttgct attcactgtt
     2341 cgattattta agctggggct tttaaaaact tggagatgtc aaataaaaaa agggagaatg
     2401 tttgtaaaaa ataaaaaata aaatctaata aatatcgaag aaaatacatt tctgacataa
     2461 tcaattcgat caataagttt ttgcaaagaa tttagcccta aatatcctaa atagatattg
     2521 acatcgagac ttaatttgct tccgtgtgaa cttgaagttc tgttgattaa gaagactcca
     2581 aattcttttc catttgttcc catattgatt gtgcaaacga ataaggatca gatcttcggc
     2641 taatgtgaat atttttctca tactatttaa ttattaaaac tgagagaccc ataaacattt
     2701 caattaaagt ttttttaata gagttttttt gagattaaaa caacaatcat ttttatgatt
     2761 tcaacacctc aagacttgct cccacagcaa tccagaatta aaaggtcacc agctgaagaa
     2821 ttaaagtatc agctaaacaa aaagtatgag aaatatgtgt ttgtacccgt tcgaggaacc
     2881 atctcaccca ttacaaaagc agaatttatg actagatttt tttagaagta ctagaaagac
     2941 ttccagaagt agtatccaac aaggttcgga gttgacatag ctgatctcct taacgattac
     3001 gaggaaatct gtcaccagat cacttctttt tcaagcaacg tcaaaggaaa gatttattaa
     3061 aaagagattg cgtaagaatt gcaatagctg agtcaaattg gcttgaatga atattattaa
     3121 agactcttgg atgacatgag taaaaaaaca gaaagagatt ttaactggca gaatttggag
     3181 tctcatattt ttggaagata atttagaaac aggagatagg cactttattt cgtttaagtg
     3241 aataggattt tagctaaaaa aaacgaatag ggtctaattg aagaggtaat cgagatcgga
     3301 caattaaaga ctaattgctc ttcatgcaag gctccctgga cattttacca tgaaaatagt
     3361 cctttgtgtt gttcgaatct atcatgtgtt gatcatcaac cagcaagaaa aagaattgtt
     3421 ttattctaaa gacagtttag aataactcca atgtcctttt ccggttatac aatcgattgg
     3481 gatgaaagtt tgaatcaata tgtcgaccat gacataaaaa tctatcaaaa gtagttccat
     3541 agctcaaaca atatcttatc aacaataaag aaatcctgta aaaattactc ttcaaacatt
     3601 gaatggtgtg gaagtctggc taagataaat ttgaacacca aacaacttag acttcttcca
     3661 aaagtggaag cataaggggt cagtggtgct atttcttatt aactttcctc ttgtttgcaa
     3721 attcaataca aaggaaacaa ttcgacagtc gtttctctga gaaaaattcc agaaggaagt
     3781 gttgtggctg tttgcggagg aagggtaatt ttcttttaac atagatgtac agatcagagt
     3841 aatatccaat gaaatcaaaa atttgcgatg atgaggaaag attgatgatt tcttttggat
     3901 ggatcaggaa ttcttagctg attttaatct cagactagga tagaaatgtt gttttctata
     3961 tgaggaatga aaaaaatggg aatttgcaag cctaatggat cctgattgat gaaagaaacc
     4021 ctactctgag tttagttcta accgcaaaca gagaaatcta agccaattaa gagttgacgt
     4081 ggaacaaaag aatgattcag gtgcaagaaa gaggaaacat tatgaaaaga aagccattgt
     4141 ttggcgaaag catcagactg aatccagtta atgaatgatt aaataagtct catcaaatca
     4201 tcaatcctta attttctttg caattcctat attttgtttt gggtctttgg tttttggaat
     4261 gtagaacgtt tttgttattt gatcaaagta accaaacttt atctcatcat ttttagagtg
     4321 atcattgtat tgcataaatt acaataagcg atgtttttcg ggctgctcat gtattctcga
     4381 gatgacgaaa caacgaaatt atcttaaatt atcatttcat aaaatcaatc aattttattt
     4441 gcccgcctca cttaaggttt agcattattg actgggatat aaaaagttct ggtcactgag
     4501 tcaaagaacc ctaatttgac ttgatcattc ttttcgttga attacgtgaa ttcccgtgtc
     4561 atacatgtaa agtcattctg aatggacgac acgaaattgg cttgttcggt ggagatagga
     4621 tgttgtgttt attccgagtc tgacattatt ataattatta ttatgtggca acaatgttca
     4681 taaatcaacg ctcctctcat catagcattt ttaagcatgc tattctaggc tctaaccttc
     4741 atactggttc tcaaagccca taacggggag cgacaaacag atgagggaaa tcaaaggaag
     4801 tagaaaaata gttgattatc aatattaatc atttatcata tctatttcat tgagaatctc
     4861 aagaacgaat gttctaaact gtctaagtta gctaatgttt aatctgagct tgatgttgtc
     4921 gattgtgact tctgaggttg tggctccgat gatgaataag attatgtata ttatcattgt
     4981 ttataacttt tgggatcaaa tcgccatgtg tatgatttta aacatgactg gcacagcatt
     5041 cataattggg aaattgattg ctgtcgtttc ttagtccaca tatacttcaa atctcagggt
     5101 tctaagattt cctaaagacc atgaagctgg tgattaattt cgatatatta aatctctctt
     5161 tgtcagacca ttttcaacaa tagcgtttgt ctggaccaga ttcatgacaa ttatctttgt
     5221 cgttgcaact ccattaaatc caataaatcc tgagtaattt cttagggcta ttgaaattgt
     5281 caatggaaaa ttatcaccca caaaatcctg caggattgtc gtgaatcctt aagttactaa
     5341 aaacaagaat ttttagtttg gtgtgaggac gggaacatca tattgccaag ccaaagtagc
     5401 atctgatgat gaaacaagag gctgaagctt aaagttaaaa tattacgtaa tctgattaga
     5461 agaagcagct tgaggactaa gtagtcttgt gtcttgagtc gggtcggctt ttttttcttc
     5521 tctgcggatt gctgaattga ggaatggatt gacggctttc ataataatat atatattatg
     5581 caataattcg ctacgagtga acgccaagcc taagcagaat aataactctg gaagaaaaat
     5641 aatttctacg atttaaaaaa ttaaatcgca cttccgaaag aagcataaaa ataaatctca
     5701 aaggtcattg aagaaagtta tgacactctc aaaaaaataa ataacagaga agcttaaatc
     5761 atgatataac gcaattcagt tcggtctgtt gaagagaaaa cctttaagcg agggtcgtat
     5821 ttgacggtgt aaagcatgac agttcttcaa tatacttaat tctcaatcat gggttagata
     5881 gctagattcg tatataacat gttgactaat cttatataaa ttgagtcgca agttgatcca
     5941 ataacacatg aattaattaa aataaattaa gtcccagtga tggagatagt gacaaaaatc
     6001 ttataatgtt aaaatgtttg gcaacaattc aacatggtga taaacattta tgacgatgac
     6061 atttaaaaga gagccttatt agctgaattt atatttacaa ttttatcaaa aatcttccga
     6121 cacgcaacat aattgggttt tgctatcagt atcacataat tctaaaaggc tgtattcaat
     6181 taaaagatcc ttgataatct gtcagctgat gatgttgcga aagcccaaac tgaagctttt
     6241 atattgaatc gctaaaagaa tgcaaatgct aattttcacg tcgaaatagc aaaatactat
     6301 agaggaaaat ttattgaatt actaaaaaaa aagagggaat cttatacgaa gaaataaaag
     6361 attcatttct ggtctctttt gtaaccgagc aaatttccga atatgaatta aatgatccaa
     6421 gcataaattt cagggcagat agacaccata aagccgataa tgttgaaaag caaggacaag
     6481 tagagtcttg tataactttt taatcatatc aacgacgttt aaccaaccaa atagaagcgt
     6541 taaagtacaa agaaaatgaa gtaataattg aattctacga ttaaaactaa gaaatttata
     6601 gctaaaatca gacgatcatg atattagttt tttaaacata ttgaatcctt atatcccagt
     6661 tgaaaccaaa ttttcgaagt tacttggtgt tattttttgg agatgctgat tcatactaga
     6721 aacttggaaa ttattctgtt taagcattta agcggtgctg atggctttct gggcatattt
     6781 attagttgtg ttattcttcg cgaagttgat acccttgtcg atcaattttg atttgttgtt
     6841 ggtgacatag tgcttaccga tttttttgat gttatcaaat agcttcataa taataatata
     6901 aatgtcatca ttccaattac gacccacaac ttaaacacct gagtaactga cccaacacgg
     6961 taattaatat tatttaatca accccgaaga tctataagtc tttcctggtc aatagattaa
     7021 ttacgtttcc aattataagt tagcaagtct ccctgtcgat aagatggttg aagtatcagg
     7081 aactgatgat atgataacat aataagttta atccaaccac aaacattatt ttcaaaatga
     7141 tattggcatg tgtttatctc tctttatgaa atacatggga aaaggaaaga aaacaataca
     7201 caagggaaag cgtttgttca aattcagttt aaaaaaggga ctttagtcat cttcttaaat
     7261 tctagatatt ctaccctttt tgttaaaaga tgtaaaaaaa aaaaatatcg cagccgaatt
     7321 caaaaggagt taaaataata gcatcgataa atcagcgtca aaacttgtta aaaggtcttt
     7381 cagtaagagc ccaaagcctt ttacaacatt ttgttgatag aaatattaat ttcatcttca
     7441 ttaacaagaa tcttcttctt tttcaaaaaa gaaacaacca tcgttattat tatctttctt
     7501 taattgttgt caaacttcaa taattgttct tttgtgtgca acgacccacc ccattcaaat
     7561 aaattagccc caattttatt ttatcctaat tcaatgaaaa ccttcgaatt atcttttgat
     7621 taaagaagta tactattgct gttttgggtt ctcattgatg attgtttcca attgttttca
     7681 tcaataattt tcccatcaac tattcttaag cttttgattg aaaatatgat ttttatgaat
     7741 ttagtgatca tttacttaac ttcgtactct tactgattat cgatgaagat tttagtccat
     7801 tctttagccg gttactttat cattctatac acattttgtt attttcctat catgttctgt
     7861 attgaacttt ctaatttttt tgaagcctat tcgagcgcat tattttaatc agcataaaac
     7921 cggacttttt ataagtgcga tagcatggct tacttaatta cggaatctat ggcttattat
     7981 cttaatccta tttattatta tttctgaaca gcatacttac tgaattaacc aaatacatat
     8041 ttctttggtc ccggatctta atctgaggga tcatttgagg gatcccttag aggtcgtttc
     8101 cttatacgct ttcgagaaac cttatttttg atttatgctg ctacttattt ttaaactacg
     8161 tcacctttat caacattttg ttcttattca gcatcttcgt cttcgtcatc ctctccttct
     8221 tcgtcatcct ctccttcttc gtctccttct tcttcgtctc catcttcttc tttttcgtct
     8281 tctgcagcat catcttcatc atcattattt tattcatcgg gttgttatta aatagcttac
     8341 tt
//
LOCUS       DAOQCW010000111         8248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_3383_length_8248_cov_5.405224, whole genome shotgun sequence.
ACCESSION   DAOQCW010000111 DAOQCW010000000
VERSION     DAOQCW010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8248
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3383_length_8248_cov_5.405224"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PLS16_09430"
     CDS             <1..695
                     /locus_tag="PLS16_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61343.1"
                     /translation="SDFNSTIHNSFTSNGFHILHTEPDLYLILKPDWTNFEDYLNSFS
                     SKYRVRAKKVLRESESIQNKILTYNDVLFYKQEIEQLFENVMLNAQFFLSPLNCNFFV
                     QKHEKSDNICQIHGYFLNEKLVGYSNIYICNKIIHVHYIGLDYKINSNYKLYNRMLLD
                     VVKIGIEHKVEKIHFGRTATEIKTTIGAEPSYFKSFLKIRNPIYNTIATYFLKKILPP
                     KFTVRHPFKLNY"
     gene            complement(705..1724)
                     /locus_tag="PLS16_09435"
     CDS             complement(705..1724)
                     /locus_tag="PLS16_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61344.1"
                     /translation="MMINQFKKICLYLFIFLILSCQKPKKALLKDSYENWMQELIEEY
                     PNSDVTLRSIMMPAAHDAGMYDLTKCTFGANFCNTATQSLSFKEQLEQGIRLFDVRPI
                     LVGKSYYTQHATDCNGLGCRGASMTDICNDINLFLEDHAELVILELSHFCGITIYDTT
                     FINYLNTILGDKIYKESTIIERPLINRPLKEIIGNSGKGKVLLVFDGILDNFEDKAKG
                     KFPQTIFNKNNSWTNSHYLSDMLKGQLENYRNYQNEENSLFQFSWQITQNEIQAISCA
                     LNPQATSIKQGADSANVQIAPVIDSLIKNNQIRKGRIPNVIYLDFSGKFVMEKCLEVS
                     KLNLE"
     gene            complement(1783..2835)
                     /gene="lpxB"
                     /locus_tag="PLS16_09440"
     CDS             complement(1783..2835)
                     /gene="lpxB"
                     /locus_tag="PLS16_09440"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNJ61345.1"
                     /translation="MTYFFITGEESGSNYAANVIHHLKKLDAASKFYGLGGESMQHEG
                     CELIIPLEQLSFMGFSAVLKNIFTIRKNFKETQKSILKLQPDVVCLVDYAGFNLRMAK
                     WCKQNGFKTIYYILPKVWAWNESRLKKLQAYCDELISIFPFEVDYFKQKNISIHYFGN
                     PLAELISTNFNSESKTEIILLPGSRKQEIKALMPVFIELANLMQNEQFKIVAMQKLQK
                     LYPKLIPKNMEFIFDTVSNVSQNAKFAVVCSGTATLEIALLNIPQVIVYKTNFINYQI
                     AKQLIKVPFIGLPNLIAGKQIAPELIQNNCTAEQIKNFIEISSTKNIYAILEHKLATQ
                     NTSEQVAKLLMDKANA"
     gene            2956..5229
                     /locus_tag="PLS16_09445"
     CDS             2956..5229
                     /locus_tag="PLS16_09445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNJ61346.1"
                     /translation="MQLNLLFQKYGANRIKIIFYFIYLSCLFSNIQAAEINLDNTITT
                     QPLPKFFTGAGMADISSALNHDTSGNQITNSYLNSEYLKLCKALKISDYRFPAGTAGN
                     FYHFYGKGSGIDTTEVYCNPVYYGIRNKSKLQQFVNSYKFEQNLDKNYAYYFSDFMHE
                     IQKDMPDAGFYYHLNSHTHFYKGQLKQLSPAIQELINQYFEYNNTLLNRKYDEFINSV
                     DTNQIKIALNLLESLKNDSRISDLKQLLVSDANFQSSFQENINSIRYFEAQKLNLKGV
                     EIGNETEAEYVLFDDDYTHYPYSCNVDEDSEVNPFTKIQLRDYMEGLIKNWIITTLYA
                     DSIHTNFNIPIGLTISGFRGNIGFDKNDNPYFIKQFGVLEKKAFFWSKFFGNESKIDA
                     YIPHIYSQKVLECNNYTAKLDTTPSEQINKIAIDVIENFSDLSLPYVLKHINQTVNGK
                     PLWITEWNFNMKSYVPNTFMHAYFLHRAISKNIEFYENNLYNIQSWLLHYLSATYYGY
                     ALVKSGYYGAKYTINKMLPYESFYMWGNTLNDQSKKLNINLSSQNNQAKEVQCFINKD
                     KTKIFIHYNNISDNDDILLLNNITINDNAKNYKIKSVNTHILAAQTLLASNYTECQAV
                     KNQIPPNSYTIIEDSLMTNFDTIQLPLKSIGQITLQIEANSSTKTKHSKKKENAIKIY
                     PNPSNYYLNIEIQNFNVDENYTLKIFDTSGKIVKVFNDVKSYNLIDIHQLNYANYIIS
                     LFNDNELVQSQKFTKKN"
     gene            5282..6841
                     /locus_tag="PLS16_09450"
     CDS             5282..6841
                     /locus_tag="PLS16_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNJ61347.1"
                     /translation="MPQLIQIQLSPQEAKNDLQIKINIANHLSLTNEGFIYVWKKRSI
                     DARKKNIKINATFEVFFLNENYQETTFKFQSKDISQAPTIHIIGAGPAGLFAALQAIE
                     SGFKPIVFERGKDVRERRRDLAKLNKESIVNPESNYCFGEGGAGTYSDGKLYTRSKKR
                     GNVQKAMEWFVAFGADSNILVDAHPHIGTNKLPQIIVAIREMIIACGGEIYFNSKLTD
                     IHLERNNINGITINNKNYHSIEHLVLATGHSARDIFYLLHQKGIKIEAKGFALGVRIE
                     HSQNLIDSIQYHGQLSGELLPPASYSLVTQVNGRGVYSFCMCPGGIIAPCATEQDEVV
                     TNGWSPSKRNNPYSNSGIVVSIEPEDFPNHESNPFVCLEFQKMVEQRCWEIGGKTQSV
                     PAQRLLDFINGKVSEDLPRSSYQPGYELADLNEVLPKIISKNLKKAFLDFGKKMPQFL
                     SNDAVLHAPESRTSSPIKIPRDSETLQHLQIQNLYPCAEGAGYAGGIISAAVDGMNCV
                     SKIAELKSIEI"
     gene            6846..7469
                     /locus_tag="PLS16_09455"
     CDS             6846..7469
                     /locus_tag="PLS16_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HNJ61348.1"
                     /translation="MQKKYYNNIAGQDIGRIIAISDGVFAVALTLLVLEIRVPLLDII
                     HSEKELVVQFALLKSKFLVYLLAFMTTGIFWLGHSSQYKHIEKSDRNLNWINLIFLLF
                     VTMLPFTTAFLGDYTNFKFPIFIYWLNIFMLGVLLYINWNYAYKKGFVNENTKEDINN
                     PLRKRIIYAQTLYFIGALLCFINPYISITFIILIQINYAFGFVSWLG"
     gene            7595..>8248
                     /locus_tag="PLS16_09460"
     CDS             7595..>8248
                     /locus_tag="PLS16_09460"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNJ61349.1"
                     /translation="MNTKQSEILFEKAKTLMPGGVNSPVRAFKSVGGTPIFFQKGKGS
                     KVTDVDDNEYIDFCNSWGPLILGHCNPEVENAIIETVKNGTTFGAPTSLENELAELII
                     SNHKFIEKIRFVSSGTEAVMSALRLARGVTGRSKIIKFEGCYHGHSDSLLVKAGSGLI
                     TFGASTSAGVPEAFANETIVLPLNDEEKLTQTLQQLPKDIAAVIIEPIPANNGLLLQS
                     "
BASE COUNT         2999 a         1207 c         1139 g         2903 t
ORIGIN      
        1 tttctgactt taattcaact atccataata gttttacatc aaatggtttt catattttac
       61 atactgagcc agatttatac ttaattctaa aaccagattg gacgaatttt gaagattatc
      121 taaattcttt ttcatcaaaa tatagagtac gtgctaaaaa agttctaaga gagtctgaat
      181 ctattcaaaa taaaattctg acatacaatg acgttctttt ttataaacaa gaaattgaac
      241 aattattcga aaatgtaatg ctaaatgcac aattttttct ttcaccatta aattgtaatt
      301 tttttgtaca aaaacacgaa aaatccgaca atatttgtca aattcatggt tattttctta
      361 atgaaaagtt agttggatat tcaaatattt acatctgtaa taaaatcatt catgttcatt
      421 atattggatt ggactataaa ataaactcaa actataaact atacaataga atgttattag
      481 atgttgtaaa gattggaatt gaacataaag ttgaaaaaat acattttggg agaaccgcta
      541 ctgaaatcaa aaccactatc ggtgcagaac cttcttattt taaaagcttc ttaaaaatta
      601 gaaatcctat ttataatacc attgcaacct atttcttaaa aaagatttta ccacctaagt
      661 ttactgtccg acaccctttt aaattaaatt attaatctct ataattattc taaattcaac
      721 ttactaactt ccaaacactt ttccattaca aacttccctg aaaaatctaa gtaaataaca
      781 ttaggaattc ttccttttct aatctgatta tttttaatta acgaatcgat tactggagca
      841 atttgaacat tagcactatc tgcaccttgt ttaatggaag tcgcttgggg gtttaatgca
      901 caactaatgg cttgaatttc attctgtgta atctgccaac taaattgaaa caaagaattt
      961 tcttcattct gataattccg ataattttct aattgtcctt ttaacatatc acttaaataa
     1021 tgactatttg tccaagaatt atttttatta aaaatagtct gaggaaattt acctttagct
     1081 ttatcttcaa aattatccaa aattccatca aatactaata acactttacc ttttccagaa
     1141 ttgccaataa tttctttaag tggtcgatta attaatggtc gttctattat tgttgattct
     1201 ttataaattt tatctcccaa aatagtattc aaatagttga tgaatgttgt atcataaatt
     1261 gtgattccac aaaaatggct taattctaat atcaccaatt cagcatggtc ttctaaaaat
     1321 agattgatat cattacaaat atctgtcata cttgcacctc tacaacccaa tccattacaa
     1381 tctgttgcat gttgtgtata atacgatttc ccgactaaaa taggacgaac atcaaaaagt
     1441 ctaatacctt gttctaattg ctctttgaag ctcaatgatt gcgtagctgt attgcaaaaa
     1501 tttgcaccaa aagtacattt agtcaaatca tacatgcccg catcgtgtgc agcaggcatc
     1561 ataatacttc tcaatgttac gtctgaattg ggatattctt caattaattc ttgcatccaa
     1621 ttttcataag aatccttcaa taaagccttt ttaggctttt gacaagataa aatcagaaaa
     1681 atgaatagat acaaacaaat tttcttaaat tgattaatca tcataacaat tcataataat
     1741 taaaaataaa acctcaagta aagttataaa aaatttatat aactatgcgt tagctttgtc
     1801 catcaacaac ttagcaactt gttctgatgt gttttgtgta gccaatttat gttctaaaat
     1861 tgcataaata tttttagttg aacttatttc tatgaagttt tttatttgtt cagctgtaca
     1921 attattttga attaattctg gagcaatttg ttttccagca attaaatttg gcaatcctat
     1981 aaatggtact ttaattaact gttttgcaat ctgataatta atgaaatttg ttttataaac
     2041 aataacttgt gggatattta gcaaagcaat ttctaaagtt gctgttccgc tacaaaccac
     2101 agcaaactta gcattttggg acacatttga aaccgtatca aaaataaatt ccatgttctt
     2161 tggtattaat ttaggatata acttttgtaa tttttgcatt gccacaattt taaattgttc
     2221 gttctgcatt aagtttgcaa gttctataaa cacaggcatt aatgctttaa tttcttgttt
     2281 tctacttcca ggtaacaaaa ttatttctgt ctttgattca ctgttaaaat tagttgatat
     2341 taattcagcc aatgggtttc caaaataatg aatggatata tttttttgtt taaaataatc
     2401 tacttcaaat ggaaaaattg aaatcaactc atcacaatat gcttgtaatt tttttaatct
     2461 actttcattc caagcccaaa ctttgggcaa aatataataa atagttttaa acccattttg
     2521 cttacaccat tttgccattc gcaaattaaa gccagcataa tccactaaac aaaccacatc
     2581 aggttgtaat tttaaaattg atttttgagt ttctttaaaa ttttttctta ttgtaaatat
     2641 atttttcaaa acagctgaaa atcccataaa actcaattgt tctaaaggta taatcaattc
     2701 acaaccttca tgctgcatac tttctcctcc taatccataa aatttagaag ctgcatctaa
     2761 cttttttaaa tgatgtatta cattagcagc ataattactg cctgattcct ctcctgttat
     2821 gaaaaaatag gtcattaaaa agatttgcaa ttggtaatat tacaaaataa atttgtttta
     2881 cttcaataga atatcgcttt aagattctcg ctaactttca caataataaa ctatatttga
     2941 gcattcaact ttattatgca actaaatcta ttgtttcaaa aatatggtgc aaatagaata
     3001 aaaatcattt tttattttat ttatttatct tgtttattta gtaatatcca agctgctgaa
     3061 ataaatttag ataatacaat aactacacaa ccattgccta aattttttac aggtgcaggt
     3121 atggcagata tttcgagtgc tttgaatcac gatacaagtg gaaatcaaat aaccaattct
     3181 tacttaaata gtgaatattt aaaattgtgt aaagctttaa aaatttccga ctatagattt
     3241 cctgctggaa ctgctggaaa cttttatcat ttttatggca aaggaagtgg aattgatact
     3301 acagaagttt attgcaaccc agtttattat ggtattagaa acaaaagcaa attacaacaa
     3361 tttgtcaata gctataagtt tgaacaaaat ttagataaaa attatgctta ttattttagt
     3421 gattttatgc acgaaatcca aaaagatatg ccagatgctg gattttatta tcacctaaat
     3481 tcccatacac acttctataa aggtcaatta aaacaactat ctccagcaat tcaagagttg
     3541 ataaatcaat attttgaata caataacact ttactaaata ggaaatatga tgaatttatc
     3601 aattccgtag atactaatca aataaaaata gcccttaatt tattagagtc attaaaaaat
     3661 gatagtagaa tttcagactt aaaacaatta ttagtcagtg atgctaattt tcaaagcagt
     3721 ttccaagaaa acatcaattc cataagatat tttgaggcac aaaaattaaa cttaaaaggt
     3781 gtagagattg gaaatgaaac agaagctgaa tatgttttat ttgatgatga ttacacacat
     3841 tatccttatt cttgtaatgt tgacgaagat tcagaagtta atccatttac taaaattcag
     3901 ttgcgtgatt atatggaagg cttaataaaa aattggataa taacaacttt atatgccgat
     3961 tccattcata ccaattttaa tattccaatt ggattaacta ttagtggatt ccgaggaaac
     4021 attggttttg ataaaaatga taatccatat tttattaagc agtttggcgt attggagaaa
     4081 aaagcatttt tctggagtaa attttttgga aatgagtcta aaattgatgc ttatattcct
     4141 catatctatt cacaaaaagt attggagtgc aataattata ctgcaaaact tgataccact
     4201 ccatctgaac aaattaataa aatagctatt gatgttattg aaaatttctc agatttatct
     4261 cttccttatg ttctaaagca cattaaccaa acagtaaatg gcaaaccact ttggattacg
     4321 gaatggaact ttaacatgaa atcttatgtg cctaatactt ttatgcatgc ttattttctg
     4381 catcgtgcta tttcaaaaaa tattgaattt tatgaaaata atttatacaa tatacaaagt
     4441 tggttattgc actatctatc agctacttat tatggttatg cgttagtaaa atcaggatat
     4501 tatggagcta aatatactat aaataaaatg ctaccttatg aatcattcta tatgtggggc
     4561 aatactttaa acgaccaatc taaaaaatta aatattaatt taagcagtca aaataatcaa
     4621 gcaaaggaag tgcaatgttt tataaataaa gacaaaacaa aaatttttat acactataat
     4681 aacatttctg acaacgatga tattttgtta ttgaataaca ttacaataaa tgataatgct
     4741 aaaaattata aaatcaaatc tgtcaataca catattttag cagctcaaac attattagca
     4801 tctaattata cggaatgtca agctgtaaaa aaccaaattc cacctaatag ttatacaata
     4861 attgaagatt cattaatgac aaattttgac acaattcaat tgccattgaa atccataggt
     4921 caaattactt tacaaattga ggcaaattct tcgactaaaa ccaaacattc taaaaaaaaa
     4981 gaaaatgcca taaaaatcta tccaaatcca agtaattatt atctaaatat agaaattcaa
     5041 aactttaatg ttgatgaaaa ctacactcta aaaatatttg atacatcagg gaaaatagtt
     5101 aaagttttca atgatgtaaa gtcctataac ttaatagata ttcatcaatt aaactatgca
     5161 aattatatta tatcgttatt taacgataat gagctagttc aatctcaaaa gtttaccaaa
     5221 aagaactaga aatatagata ataataatat tctactttta atatctttgc attttaattt
     5281 tatgcctcaa ctcattcaaa tacaacttag tcctcaagaa gcaaaaaatg atttgcaaat
     5341 aaaaataaac attgccaatc atttatcgct tacgaatgaa ggctttattt atgtttggaa
     5401 aaaaagaagt atagatgcac gtaaaaaaaa tataaaaata aacgccactt ttgaggtatt
     5461 ttttttaaat gaaaattatc aggaaacaac ttttaaattt caatctaaag atatcagcca
     5521 agcaccaaca attcatatta ttggtgcggg acctgctgga ttatttgctg cgttacaagc
     5581 tatagaatct ggttttaagc ctattgtatt tgaacgtgga aaagatgttc gtgaaagaag
     5641 acgagattta gcaaaattaa ataaagaaag tattgtaaat cctgagtcaa attattgttt
     5701 tggcgaagga ggtgcaggta cctattccga tggcaaatta tatactcgtt ctaaaaagcg
     5761 tggtaatgtt caaaaagcaa tggaatggtt tgtggcgttt ggtgcagatt caaatatttt
     5821 agttgatgcc catccgcaca ttggcactaa taagttacct caaatcattg ttgctattcg
     5881 tgaaatgatt attgcttgtg gtggtgaaat ctatttcaat tcaaagctta cagatattca
     5941 tcttgaacga aataacatca atggaatcac aatcaataat aaaaattatc acagtataga
     6001 gcatttggtt ttagcaacag gacattctgc cagagatatt ttttatctat tacatcaaaa
     6061 aggaataaaa atagaagcca aagggtttgc acttggcgtt agaattgaac atagtcaaaa
     6121 tttaattgat agcattcaat atcacgggca actaagtgga gaattattgc ctcctgcttc
     6181 atattcattg gtaactcaag taaatggcag aggtgtttat tctttttgca tgtgtcctgg
     6241 aggaataatt gcaccttgtg ccaccgaaca agacgaagtg gttacaaatg gttggtcgcc
     6301 tagcaaaaga aacaatccat attcaaattc tggaattgtt gtaagcattg aaccagaaga
     6361 ttttcctaat cacgaaagta atccatttgt ttgtctggag tttcaaaaaa tggttgagca
     6421 aagatgttgg gaaattggag gtaaaacaca aagtgttcct gcacaacgtt tattagattt
     6481 tattaatgga aaagtgtctg aagatttacc tagaagttcc tatcaacctg gttatgaact
     6541 tgccgatttg aatgaagttc tacctaaaat cataagtaaa aatctaaaaa aagctttttt
     6601 ggattttggt aaaaaaatgc ctcagttttt atcgaatgat gctgtattac atgcacctga
     6661 atctcgcact tcctctccta ttaaaattcc tagagactca gaaaccttac aacatttaca
     6721 aatccaaaac ttgtatccat gtgcagaagg tgctggttat gctggtggta ttatttctgc
     6781 tgctgttgat ggaatgaatt gtgtttcaaa aattgctgaa ttaaaatcca tagaaatata
     6841 acaatatgca aaaaaaatat tacaacaata tcgcaggaca agatataggc agaattattg
     6901 ctattagtga tggtgttttc gctgttgctt taactttatt agttttggaa atacgagtgc
     6961 cactcttaga tattatccat tcagaaaaag aattagttgt tcaatttgca ttattaaaat
     7021 ctaaatttct agtgtattta ttagcattta tgactactgg gattttttgg cttggacatt
     7081 catcgcaata caaacacatt gaaaaaagtg atagaaatct aaattggata aatttaatat
     7141 ttttactttt tgttacaatg ttgccattta ctactgcttt tttgggcgat tatactaatt
     7201 tcaaatttcc aatttttatt tattggttaa atattttcat gttaggtgtt ttgctttata
     7261 tcaattggaa ttatgcatac aaaaaagggt ttgtgaatga aaatacaaaa gaagacataa
     7321 ataatccatt aagaaaacgt attatctatg cacaaacatt atattttatt ggtgctttat
     7381 tatgttttat taatccgtat attagtataa cttttattat tttaattcaa attaattatg
     7441 cttttggttt tgtctcttgg ttaggatagc cattgttcta attttaatat ttttcttgcc
     7501 aaaacataaa attactttaa gaatctttat ctgttgataa ttgtttttaa tagacttatt
     7561 aagtaatttt gtaaatatct taaatgactt aattatgaac acaaaacaat ctgaaatatt
     7621 atttgaaaaa gcaaaaactt taatgccagg tggtgtaaat tctcccgtaa gagcttttaa
     7681 atcggtaggt ggcactccta ttttctttca aaaaggaaaa ggttcaaaag ttacagatgt
     7741 tgatgataat gaatatattg atttttgtaa ctcttgggga cctttaattt taggtcattg
     7801 taatcccgag gtagaaaatg caataattga aactgtaaaa aatggaacta catttggagc
     7861 accaacatca ttagaaaatg agttagcaga attgataatt tccaatcata agtttataga
     7921 aaaaattaga tttgtaagtt cgggaactga agctgtaatg agtgcattgc gtttagcaag
     7981 aggtgttaca ggtcgttcta aaatcattaa gtttgaaggt tgttatcacg gacattctga
     8041 ttcactttta gtaaaagcag gttctggatt aattactttt ggggcttcaa catcagctgg
     8101 ggttcctgag gcatttgcca atgaaactat agttttacct ttaaatgacg aagaaaaact
     8161 cacacaaact ttacaacaat taccaaaaga tattgcagct gttataattg agccaattcc
     8221 agcaaataat ggattgttat tacaaagt
//
LOCUS       DAOQCW010000112         7906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_3602_length_7906_cov_5.438925, whole genome shotgun sequence.
ACCESSION   DAOQCW010000112 DAOQCW010000000
VERSION     DAOQCW010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7906
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3602_length_7906_cov_5.438925"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PLS16_09465"
     CDS             complement(<1..511)
                     /locus_tag="PLS16_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61350.1"
                     /translation="MRKRILLLMAICMMITSTIFAEMKAPRKATSAVGNSKWSLGLGM
                     EVADFYSPKMGKLKTFKTPVAFGPRIGFWRNFNASIALGIDVASYAFSSKNTDPTLPA
                     VNTYNLLYAGSAVYKFNNGYVIKEDAGVAPYIFAKIQGAWAEKAITKESVNGLGIPIG
                     AGINFKIANN"
     gene            669..1253
                     /locus_tag="PLS16_09470"
     CDS             669..1253
                     /locus_tag="PLS16_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNJ61351.1"
                     /translation="MLIDFKNLVTISIVLFAIIDILGSIPIILDIRNKVGHIDEKFAT
                     IISGSIMITFLFGGEFILKLIGIDLISFSLAGAIVIFIIGLEMTLNRNFFKEGKTSTP
                     KGTIVMPIAFPMIAGAGTLTTLLSLRSQYNMLEILIGVLINLAIIYFVIKFLPNIERF
                     FGTTGIDILRKVFGFVLLSISMKIFISNLAAIKF"
     gene            complement(1268..2218)
                     /locus_tag="PLS16_09475"
     CDS             complement(1268..2218)
                     /locus_tag="PLS16_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61352.1"
                     /translation="MAKTTENKFNSKILLFGEYSAIQNSMSLTLPYQEFSGTLAFDTS
                     DVQSKFIQTSNQDLKSFALYIEEKVESKTLPISFDIHALKSDIEKGLYFNSSIPQGFG
                     VGSSGALVAAIYTKYAKDKLSLEQTPKQEEILALKHTFAILESYFHGNSSGVDPLICY
                     LNLPLLINGKNGIEPVKVELDQEGKGAIFLIDTGISRSTEPLVNYYMQRMEEHTYREF
                     VDKQLKPFANNCIQAFLDGDKETLFAYLKLLSKFQLEHFKPMIPKFFNKLWKKGIDTE
                     AYYLKLCGAGGGGYILGFTKNLEQTKQELKGQKLELLCNI"
     gene            complement(2224..3285)
                     /gene="mvaD"
                     /locus_tag="PLS16_09480"
     CDS             complement(2224..3285)
                     /gene="mvaD"
                     /locus_tag="PLS16_09480"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR01240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HNJ61353.1"
                     /translation="MIHSFKPIKGDVAWRSPSNIAIVKYWGKYGNQLPQNPNISFTLS
                     KAYSETSVAFQPKAIENAEPNIDFLFEGKENPKFRDKIVKFIDSIKSILPFLNEFDLS
                     IQSSNSFPHSAGIASSASSMSALALCLIDIERQVSDNFTDEEFYRKASIIARLGSGSA
                     CRSVYSYMATWGYSASIPSSSNEYAIDFSAHIHPVFKTYKDAILIVSSDEKSVPSRAG
                     HALMNNNLFAEARYQQAKQNMEALLPILKTGDINKFGAIAEQEALSLHALMMCSNPSF
                     ILMHANSLKIIDLIKQFRQEKDVPLYFTLDAGPNVHLLYPKSEEEKVELFIRETLSAF
                     CENGKVIYDEVGTGPVKLK"
     gene            3399..4592
                     /locus_tag="PLS16_09485"
     CDS             3399..4592
                     /locus_tag="PLS16_09485"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNJ61354.1"
                     /translation="MKKILVLIPTIFLCFVVSFCQDANKKNSFLETEPKLNPSFLLNP
                     KKSDVDINDTAIQTKIHLLDCYFAKRVAENGFNGSVLISYKGTPILEGYYGYSDYYKK
                     TKLNKDAPFQIASTSKTLTSAAILILQQEGYLNIDDTLQKYFPNFPYKGITIKMLLNH
                     RSGLPSYLDFSETYWLNKKMYMSNADVLNQLVKFKPKIYASPDRQFKYNNTNYVLLAL
                     IIEKVSGYSYSDFLQRFIFKPLGMKNTYVFDPKNRRSNEVRGYKGSSWSEDAIVFTDG
                     VVGDKGIYSTVLDLYKWDQALYAGKLISPELQSLAFTPQSFEQAGSKNYGLGWRMKDQ
                     SDGTRIIYHNGWWHSFNTVFNRKISDGTSVIILSNHYSSSVYKIQEIWDILYGNSSVD
                     MNASE"
     gene            complement(4742..5776)
                     /locus_tag="PLS16_09490"
     CDS             complement(4742..5776)
                     /locus_tag="PLS16_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61355.1"
                     /translation="MIGDKIKLFLKVDTEKFKTSDRIGLFWLDVFMIWLVIQNLIFFG
                     FDWSFKFSLFRDLIYYISPVFYNWYEQKVHPNFADWDLIFVAIFLIEFIFRWGRAIRH
                     NTYDIWWFYPFIHWYDVLGLVPMKGAFKLLRLFRLVGMFIKLQNMGVIDLSATRAYKQ
                     YKRFRTIFVEEISDLVIAKSLSSVQDELNKGIPFSNQIIEEIILPQKEKVIEVLAKTI
                     CHTVEVVYEKHRHELKRSLDTKVIQALQENKEIDTLRYLPGLGSVFQKMLDNAVSDVT
                     FNVVDKVILDITQSENQKVIENIANDVLDNIYLQSKMHSIDSEFVVNIAHQILEVIKG
                     EVLKKQYKNQ"
     gene            5897..7537
                     /gene="carB"
                     /locus_tag="PLS16_09495"
     CDS             5897..7537
                     /gene="carB"
                     /locus_tag="PLS16_09495"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNJ61356.1"
                     /translation="MPKDTSIKSVLIIGSGPIVIGQACEFDYSGTQASRSLREEGIEV
                     TLINSNPATIMTDSVTADYIYLLPLTVDSIEKILQERQIDCVLPTMGGQTALNLCKEA
                     AEIGIWDKYNVRVIGVDLEVIETTENREAFRKYCIDLGLSVAPSIIANSMLEGKDAAQ
                     KIGFPLVIRPSYTLGGSGGGFCHKPEEFEKALAHGLKSSPVHEVLIEKAVLGWKEFEL
                     ELLRDANDNVVIICTVENLDPMGVHTGDSITVAPAMTLPDTAYQEMRNQAIKLLRGLG
                     NFSGGCNVQFALDPKTENLIVIEINPRVSRSSALASKATGYPIAKIAAKLAIGYHLDE
                     LKNQITKTTSAYFEPALDYVIVKIPRWNFSKFPGCDTTLGLQMKAVGEVMGIGRNFSE
                     ALQKACQSLEDNKIGLGADGGSSGNTEQLLASLEHPSWDRIFKIREAIDLGVPLSTIH
                     KLTQIDNWFLMQIQDLSKLDKEIKKYTLDTIPVDVLKLAKNKGYSDAQLAYLLGRIDE
                     DLVYEKRKALGIKRVYKMVDTCAAEFEAKTPYFYSTFE"
     gene            complement(7556..>7906)
                     /locus_tag="PLS16_09500"
     CDS             complement(7556..>7906)
                     /locus_tag="PLS16_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61357.1"
                     /translation="GKIVKSDYTILHNLFLVFFVDEEGDRLCGSAPGKLIRVIKELEQ
                     DLQIELMNRMNLAYLNNGQAQVVQMNDFTKLIQDGKVDASTTVFDNTVSTKELFDTKW
                     QSALKDSWLNRFLK"
BASE COUNT         2658 a         1195 c         1273 g         2780 t
ORIGIN      
        1 cattattagc aattttaaag ttaataccag caccaattgg aatacctaag ccattcacac
       61 tttctttagt tatagctttt tctgcccaag caccttgtat tttagcaaag atataaggtg
      121 caacacctgc atcttctttt attacataac cattgttaaa tttgtaaaca gcagatccag
      181 catacaataa gttataggta tttactgcag gcaaagttgg atccgtgttt ttgctagaaa
      241 atgcataaga tgcaacatca atacctaatg ctattgaagc attgaagttt ctccaaaaac
      301 caattctagg accaaatgca acgggagttt taaaggtttt aagtttaccc attttaggag
      361 aatagaagtc cgcaacttcc ataccaagac ctaaagacca tttgctgttt ccaactgcac
      421 tagtggcttt tctaggtgct ttcatttcgg caaagattgt tgaggttatc atcatacaga
      481 ttgccattag taataaaatt cgttttctca taagatactt tatttgttat aaatttaagt
      541 aaatagtttg tttgaaccaa aggtaaataa attttctaat aaacatcaaa aaaagttaaa
      601 atatttaaaa ctccaatatt taaaaaatat tagtagaata tcaagctaaa tatttaactt
      661 cgatttctat gcttatagat tttaaaaatt tagttaccat tagtatagtc ttatttgcta
      721 ttattgatat acttgggtct attcccatta ttttagatat cagaaataaa gtaggtcata
      781 ttgatgaaaa atttgctact ataatttctg gttctattat gattactttc ctttttggag
      841 gtgagtttat tttaaaacta attggtattg atttaatttc cttttcattg gctggtgcta
      901 ttgttatctt tataattggg ttggagatga ccttgaatag aaattttttc aaagagggaa
      961 agacatctac gcctaaaggc accattgtaa tgccaattgc tttccctatg attgctggtg
     1021 ctggaacttt aacgaccttg ctttctttac gttctcaata caatatgtta gaaattctaa
     1081 ttggagtact gattaattta gccattattt attttgttat aaaatttctt ccgaatattg
     1141 aacgattttt tggtacgaca ggaattgata ttcttagaaa agtatttgga tttgttttac
     1201 tctccatttc aatgaagatt tttatttcaa atcttgcggc aattaagttt taaaagtata
     1261 atttaagcta tatattacac aataactcta gcttttggcc tttgagttct tgttttgttt
     1321 gttcaaggtt cttggtaaat cctagaatat aaccaccacc accagcaccg cacaatttta
     1381 agtaataggc ttcagtatca atacctttct tccataattt attgaaaaat tttggaatca
     1441 taggcttaaa atgttccaat tggaatttag aaagcaattt taaataagca aataaggttt
     1501 ctttatcacc atctaaaaat gcttggatac aattgtttgc aaatggcttt aattgtttat
     1561 cgacaaactc tctataagtg tgttcttcca tcctttgcat ataataattt accaaaggtt
     1621 ctgttgaacg agaaatgcct gtatcaatta agaatattgc tccttttcct tcttggtcaa
     1681 gctctacttt gactggttcg atgccatttt ttccattaat tagtaaaggt aaattgagat
     1741 agcaaatcag cgggtctaca ccagaactat tcccatggaa ataagattct aatattgcaa
     1801 aagtatgttt aagtgctagt atttcttctt gttttggtgt ttgttctaaa cttaacttgt
     1861 ctttagcata ttttgtgtaa attgcagcga ctaaagcacc tgaacttcca actccaaagc
     1921 cttgaggaat agaagaatta aaatataagc ctttctctat atctgatttt aaagcatgaa
     1981 tatcaaaaga aatgggtagt gtcttagatt ctactttttc ttcaatatat agtgcaaaac
     2041 tttttaaatc ttgattagaa gtttgaataa atttactctg tacatcagaa gtatcgaacg
     2101 ctaaagttcc actaaattct tgatagggca aagtcaaact catagagttt tggatagcac
     2161 tatattcacc aaataaaagg attttagaat tgaacttatt ctcagttgtt tttgccatga
     2221 ggcttatttt agtttcacag gtcctgttcc tacttcatca taaataacct ttccgttttc
     2281 acaaaacgct gaaagtgttt ctctaataaa aagctccact ttctcttctt cacttttagg
     2341 atataataga tgaacattag gtccagcatc caatgtgaag tacaaaggta catctttttc
     2401 ttgtctaaat tgttttatta aatcaataat ttttaacgag ttagcatgca taagtataaa
     2461 tgatggattt gaacacatca tcaaagcatg aagagaaagt gcttcttgtt ctgcaattgc
     2521 tccaaattta ttaatatctc cagttttcaa gataggcaaa agtgcttcca tattttgttt
     2581 ggcttgttgg tatctagctt cagcaaataa attattattc attaatgcat gtccagctct
     2641 actcggaacc gatttttcat ctgaggaaac aattaaaata gcatctttat aggttttgaa
     2701 tacaggatgt atgtgagccg aaaaatctat tgcatattca ttacttgatg atggaattga
     2761 ggcagaataa ccccatgttg ccatatatga atacactgaa cggcaggcac ttcctgaacc
     2821 taatcgtgcg ataatactgg cttttctata aaattcttca tcagtaaaat tgtctgaaac
     2881 ttgcctttct atatcaatta aacataaggc aagtgctgac attgaagacg cagaacttgc
     2941 aataccagca gagtgaggaa atgaatttga actttgaata cttaaatcaa attcatttaa
     3001 aaatggcagt attgatttta tgctatctat aaattttaca attttatctc taaattttgg
     3061 attttctttt ccttcaaata aaaaatctat gtttggttca gcattttcaa ttgcttttgg
     3121 ttgaaatgca acagaagttt cagaataagc tttggatagt gtaaaactga tatttggatt
     3181 ttgtgggagt tgattgccat attttcccca atatttaacg atagcaatat tagatgggct
     3241 tctccaagca acgtctcctt tgataggttt aaatgaatga atcattatta aatctgttat
     3301 ttttttgcaa aaataccttt tttttgctga actaaagtaa gataccttat ttttgtgcct
     3361 ttggaatgat ttgtgtagtt aataatatga aaaagtttat gaagaagata cttgtattga
     3421 tacctactat ttttttatgt tttgtagttt ctttctgtca agatgcaaat aagaagaatt
     3481 catttttaga aacagaacct aagctaaatc cttcattttt attaaatcct aagaagtcgg
     3541 atgttgatat caatgatacc gcaattcaaa caaagattca tttgttggat tgctattttg
     3601 caaaaagggt agcagaaaat ggatttaatg gttcggtttt aataagctat aaaggaacac
     3661 caattttaga gggttattat ggatattccg attattataa gaaaacaaaa ttgaataaag
     3721 atgcaccatt tcagattgca tctacttcta aaacactaac atctgcggct atcttgattt
     3781 tacaacaaga aggctactta aatattgatg atactttaca aaagtacttc ccaaattttc
     3841 catataaagg gattacaatc aaaatgttgc tcaatcatcg ttctggttta cctagttatt
     3901 tagatttctc agaaacttat tggttgaata agaaaatgta tatgagcaat gcagatgttt
     3961 taaatcaact tgttaaattt aaacctaaaa tttatgcttc tccagataga caattcaaat
     4021 acaataatac gaactatgta cttttggctt tgataattga gaaagtaagt gggtatagtt
     4081 attccgattt tctgcaaaga tttattttca agcctttagg aatgaaaaat acttatgttt
     4141 ttgacccaaa aaacagacgt tcaaatgaag tgagagggta taaaggcagt agttggtcag
     4201 aagatgcgat tgtttttacc gatggtgttg tgggcgataa gggaatttat tcaactgttt
     4261 tagatttata caaatgggac caggctttgt atgcaggcaa attaatcagc cctgaattac
     4321 aaagtttagc ttttacacct caaagttttg aacaagcagg tagtaaaaat tacggcttag
     4381 gttggagaat gaaagatcag tccgatggga caaggattat ttaccataat ggttggtggc
     4441 atagttttaa taccgtcttt aatcgaaaaa ttagtgatgg aacctcagtt attattctga
     4501 gcaatcatta cagtagtagc gtgtataaaa tccaagaaat atgggatatt ttatatggca
     4561 attcgagtgt ggatatgaat gcaagtgagt aaaaataata atcccggata taatacgaat
     4621 actataaccg ggatcatttc tttaaaccaa tcaaaatgaa aaaattattc tattcaatag
     4681 caataatctt atgcaaaata agtagcaatt ttcgtgccaa tattaaagat tcaacaattt
     4741 tttattgatt tttgtattgt tttttcagaa cctctccttt aataacttct aatatttggt
     4801 gtgcaatatt tacaacaaat tcactatcaa ttgaatgcat tttactttgt aaatatatat
     4861 tatctaaaac atcatttgcg atattttcaa ttactttttg gttttctgat tgggttatat
     4921 ctaaaataac tttatcaacg acattaaaag taacatcact cactgcatta tccaacattt
     4981 tttggaaaac actgccaagt cctggtaaat acctcaaggt atctatttct ttattttctt
     5041 gcaatgcttg aattacttta gtatctaaac ttcgttttaa ttcgtgtcta tgtttttcat
     5101 aaaccacttc cacggtatga cagatagttt ttgccaaaac ctctataact ttttcttttt
     5161 gaggtaatat tatctcctct attatttgat ttgaaaatgg gatacctttg ttcaattcat
     5221 cttgtacgga gcttagcgat tttgctatta ctaaatcgga tatttcttca acaaagattg
     5281 ttctaaatct tttgtattgc ttataagctc tagtggcact caaatcaata acacccatgt
     5341 tttgaagctt tataaacatt cctacaagtc taaataatct taatagtttg aaagctcctt
     5401 tcattggcac taagcctaaa acgtcgtacc aatgtataaa agggtaaaac caccaaatat
     5461 cataagtgtt gtggcgaatg gcacgtcccc atctaaatat aaattctatc aagaaaatgg
     5521 caacaaatat caaatcccaa tctgcaaaat ttggatgaac tttttgttcg taccaattgt
     5581 aaaagacagg ggagatataa taaattaaat ccctgaaaag agaaaacttg aatgaccagt
     5641 caaaaccaaa aaaaatcaaa ttttgaataa ctaaccaaat cataaaaaca tctaaccaga
     5701 aaagtccaat tcggtctgat gttttaaatt tttcagtatc tacctttaaa aataatttta
     5761 ttttatctcc aatcatttag cactctttta ggctataaaa ataaaaaagt aattaaccca
     5821 atcataataa aaaaacaaac acttactttg ttgataacta atctcaaatc cttatctttg
     5881 tgcaacttct aatattatgc caaaagacac atctatcaaa tcagttttaa ttataggtag
     5941 cggtcctatt gttatcggtc aagcttgtga atttgattat tctggaactc aagcttctcg
     6001 ctcattaaga gaagaaggaa ttgaggtaac tttaatcaat tctaatcctg ctacaataat
     6061 gacggatagt gttacggcag attatattta tcttttgcct ttgacagtag attctattga
     6121 aaagattctg caagaaagac aaattgattg tgttttgcct acaatgggtg gacaaacagc
     6181 cttgaattta tgtaaagaag ctgctgaaat tggaatttgg gataaatata atgtacgtgt
     6241 aattggtgtc gatttagaag taattgaaac tactgaaaat agagaagcat ttagaaaata
     6301 ttgcattgat ttaggacttt ctgttgctcc atcaattatt gctaattcta tgttggaagg
     6361 aaaagatgct gctcaaaaaa taggatttcc attagtaatt cgtccatctt atactttagg
     6421 tggaagtggc ggtggatttt gtcataaacc tgaagagttt gaaaaagcat tagcccatgg
     6481 tttaaaaagt tctccagtac acgaagtgtt aatagagaag gcagttcttg gttggaaaga
     6541 atttgaatta gaattgttga gagatgcaaa tgataacgtt gttattattt gtacggtgga
     6601 aaatttagat ccaatgggtg tacatactgg tgattctatt acagttgcac cagcaatgac
     6661 tttgcctgat actgcttatc aggaaatgag aaatcaggct atcaaattat tgagaggctt
     6721 aggtaatttt tcaggtggct gtaatgtgca atttgcttta gatccaaaaa ctgagaattt
     6781 aattgttatt gaaattaatc caagggtttc tcgttcttct gctttagcgt caaaagcgac
     6841 aggatatcca atcgcaaaaa ttgctgctaa attggctatc ggttatcatt tagatgaatt
     6901 aaaaaatcaa attactaaaa caacgtctgc ttattttgag ccagctttag attatgtgat
     6961 agtgaaaatc ccaagatgga attttagcaa atttccaggt tgcgatacaa cccttggatt
     7021 acaaatgaaa gcagttggtg aagtaatggg cattggaagg aatttttcgg aagcacttca
     7081 aaaagcttgt caatcattag aagataataa aattggtttg ggtgcagatg gtggaagtag
     7141 tggaaatacg gagcaattat tagcaagttt agagcatcca agttgggata gaatatttaa
     7201 aattagagaa gcgatagatt tgggagttcc attgagtacg atacataaat tgacacagat
     7261 agataattgg ttcttgatgc aaattcaaga tttatcaaaa ttagataaag aaataaaaaa
     7321 atatacttta gatactatcc ctgttgatgt cttaaagtta gcaaaaaaca aaggttatag
     7381 tgatgctcag ttggcctatt tattaggtag aattgatgaa gacttggtat atgaaaaacg
     7441 caaagcttta ggtatcaaaa gagtttacaa aatggtagac acttgtgcag ctgaatttga
     7501 agcaaaaacg ccttatttct attctacttt tgagtaaatt ctactacaac tctaattatt
     7561 tcaaaaaacg attaagccag ctgtctttta aagcagattg ccattttgta tcaaataatt
     7621 ccttagtact tacggtatta tcaaaaacag ttgttgatgc atcaactttt ccatcttgaa
     7681 taagttttgt aaaatcattc atctgtacaa cttgggcttg tccattattc aaatatgcca
     7741 agttcattcg gttcattagt tcaatctgta aatcttgttc taattctttg ataacacgaa
     7801 ttaattttcc tggagcagaa ccacataatc tatctccttc ttcatcaaca aagaaaacta
     7861 agaataaatt gtgtaaaatg gtataatctg atttgacaat ttttcc
//
LOCUS       DAOQCW010000113         7845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_3649_length_7845_cov_6.157125, whole genome shotgun sequence.
ACCESSION   DAOQCW010000113 DAOQCW010000000
VERSION     DAOQCW010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7845
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3649_length_7845_cov_6.157125"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1231)
                     /locus_tag="PLS16_09505"
     CDS             complement(<1..1231)
                     /locus_tag="PLS16_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNJ61358.1"
                     /translation="MSEILLDVKNLVTEFKTENGLVKAVNDVSFQIKKKQVVGIVGES
                     GSGKSVTSLSIMRLIPNPPGRIASGEILYHNQNNEIVDLTKLSEEEMRKYRGNEIAMI
                     FQEPMTSLNPVFTCGNQVMEAIMLHQKLDKNVAAAKTLELFEKVKLPNPQRILEAYPH
                     QLSGGQKQRVMIAMAMSCQPKILIADEPTTALDVTVQKTILELIKNLSDETDMSTIFI
                     THDLGVIAEIADHVIVMYKGKIVEQGSVLEIFSNPKHPYTKGLLACRPPLGKRLSKLP
                     VIADFMSDDNGVITEKAADMQALVDAFVVSEAQTKARWEDLQTRKKILEVKNIKTWFP
                     TKTNLFGKALDYTKAVDDVSFDVYEGETLGLVGESGCGKTTLGRTILRLIEPTSGNII
                     FNGKDITHLPQKEMRALR"
     gene            complement(1288..1995)
                     /locus_tag="PLS16_09510"
     CDS             complement(1288..1995)
                     /locus_tag="PLS16_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNJ61359.1"
                     /translation="MLQQSNISDLIFIDIETTPAYENLQKVPEYEQLLWEEKRGRYRL
                     ETENIEDFYFNNAGIYAEFGKIICISVGLVTKNNNALEYNKISFYGDDESVILKDFYL
                     FLDKSFKQNSRINFCGHNIKEFDIPYICRRSLIKGFKLHPFLAQLQTKKPWENGSIDT
                     MELWKFGDYKNYISLKLLAYSLGIPSPKDDISGKDVGRVYWQEKDLERIKTYCEKDVL
                     TTAQIILKLKGMDLLSV"
     gene            2080..3033
                     /locus_tag="PLS16_09515"
     CDS             2080..3033
                     /locus_tag="PLS16_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61360.1"
                     /translation="MEEQKQDNKSYHFLYIALIALLVGGLIFTSLKLKKQKETIVVTE
                     NQRDEVAALRSELEVKYNSAIVDIENYKSENLGLDSILRLKELDLNSKKQKIETLLAQ
                     SSNDKNALLKAQSLIKEFEAEKIAFRNTIDSLIRVNRDLGIKSDSLASELILSIEEKN
                     KLNEEKANLEEERNKMLDKIDKASILSIANIEATPIRIVKKQKEEIVKKAKDAEKLRL
                     CFDLLQNKIAPKGPTELIIRIIDPTGSTIQIQSLGSGSIIENTTGNSIPFTYSISPSY
                     DNENKQVCSYWKQNYSFPAGNYSVEIYQKGLLIGQAVFNLK"
     gene            complement(3116..4327)
                     /locus_tag="PLS16_09520"
     CDS             complement(3116..4327)
                     /locus_tag="PLS16_09520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNJ61361.1"
                     /translation="MPFNKEKNHGLNFGIQAGIISQKFNFTDAITTDGIFNDNAAVTN
                     NSRATKFDLSLGINYHWKGLNVGFSVPQLLNGKAKFRGNGMDNETSMFHYEREYLVLA
                     SYEARFGGKDKKTWMITPSLMFRKYKSIDPQIDLNVMFGYKQLVWLGAGYRTGGGYGG
                     FIKTNSAGFHVTAGAGIKERVNLFYSFEMPMKDVRTNFGYSHEVTIQCNLAKKVDKSE
                     YERNKKKVEDDIAKLNQKADEVGSKAIEAMDKGNDLADQVKNADNSIKEAFEKLNAQE
                     DNIKEIANRLDNIVFKKFGSIYFENDKAELTAEAKASLDAFKSKLSGMKGNYFIYLAG
                     NASQEGSTDYNLALSAKRSDAVKKYLEGVGISQRILLLPYGENSWVTDKQDNEADKAK
                     NRRVDIYLAGE"
     gene            complement(4517..4753)
                     /locus_tag="PLS16_09525"
     CDS             complement(4517..4753)
                     /locus_tag="PLS16_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61362.1"
                     /translation="MTDGKGIPIAIGDILEGNHNDLYRVVPQFRTMIRELNHKNICVE
                     NSILNADKSFDSKRLRRAIMRRNIIPNIKENIRN"
     gene            complement(5427..7733)
                     /locus_tag="PLS16_09530"
     CDS             complement(5427..7733)
                     /locus_tag="PLS16_09530"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ61363.1"
                     /translation="MNKIFISLALAMLMPIISAFAQEADSVNSVANKTIHGMILDAKS
                     KEPVIGAYVSSLNQKFNQVTDMEGHFMIEVPDTVTLLEISYLGYETKIIQFSNDIQHL
                     QIFLNEATKSLDGVTVVGQKATTTVSTYSTMKVENIGIGELMKAACCNLSESFETTPS
                     VDVGFTDAVSGYKQIQMLGLAGTYTSFTRENVPDIRGLAAITGLTFTPGTFVESMQLS
                     KGAGSVVNGFEGTAGQINVEWLKPFDEKSPLLVLNGYQSTQGRSEGNLIWNQVINKKL
                     STSVLLHGKGEWLKVDMNKDKFLDNPLGYTFVGSNRWFYFGEKGWDIQGGFKVVNLNI
                     TGGQKDYKRGMEQLSGTNPWGYQNTLNRIETWAKIGKVYIDKPYQSMGLQLSAIYHDQ
                     ANNFGATTYTAKQESYYANYIFQTIIKNSNHVIKVGGSLLADRYKENYKNKYFQRNEI
                     VPGVFAEYNFKWLDKINIVAGLRGDYNNLFGVFATPRLHFRYAPNEKLAIRASIGRAQ
                     RTANMLAENTGLMASNRDFYFNNELIQTSSFAKYPFKPEVAWNMGVNLTKKFLLNYRD
                     GSVNIDYYFTDFTNQVVVDFENYNRVSFYNLDGKSYSHSLQMQVDYQPVRNFDVRVAY
                     RFYDVKTTYQNNLLKRPLVAAHRMFMNLAYTTKKQWKFDYTVQWIGAKRLPEHFNDKT
                     NDIVSSSKTPSYWMMNTQINKIFKDGLMNIYVGAENMLNTMQNPLIMGASTPYSDKFD
                     ASMIWGSAMGWNLYVGFRMNLAKKDKSE"
BASE COUNT         2595 a         1390 c          999 g         2861 t
ORIGIN      
        1 ttcttagcgc tctcatttct ttttgaggca aatgagtgat gtcttttccg ttaaaaatta
       61 tgttgcctga agtgggctca attaaacgca aaatagttcg acctaaggta gtttttccac
      121 aacccgattc accaactaag ccaagtgttt caccttcata aacatcgaaa gaaacatcat
      181 caactgcttt tgtataatct aaagccttgc caaataaatt agttttagtt ggaaaccaag
      241 ttttgatatt ttttacttct aatatttttt tacgtgtttg taaatcttcc caacgtgctt
      301 ttgtttgtgc ttcacttacg acaaaagcat caactaatgc ttgcatatca gctgcctttt
      361 cggtaatgac accattatca tcactcataa aatctgcaat cactggaagt tttgataatc
      421 tttttcccaa aggagggcga caagctaaca agccttttgt atatggatgt ttaggatttg
      481 aaaatatttc taaaacggaa ccttgctcta caatttttcc tttatacatc acaattacat
      541 ggtcagcaat ttctgcaatc acacctaaat catgtgtaat aaaaatcgtg gacatatcag
      601 tttcatcact caaatttttg attaattcaa ggattgtttt ttgaacagta acatctaaag
      661 cagtagttgg ttcatctgca attaatattt ttggttggca actcattgcc attgcaatca
      721 ttacacgttg tttctgtcct cctgaaagtt ggtgcggata agcctctaaa attctttgag
      781 gattgggtag ttttactttt tcaaataatt ctaacgtttt ggcagctgct acatttttat
      841 ctaatttttg atggagcata attgcttcca taacttggtt tccacaagta aaaacaggat
      901 ttaaagaagt cataggttct tgaaaaatca tagcaatttc attgcctctg tattttctca
      961 tctcctcttc agaaagtttg gttaaatcaa caatttcgtt attttgatta tgatataaaa
     1021 tctctccact agcaattctt cctggaggat ttggaattaa acgcatgata gatagagatg
     1081 taaccgattt gcctgaacct gactcaccta caattcctac cacttgtttc ttcttgattt
     1141 gaaaagaaac atcattaacg gcttttacta aaccgttttc agttttaaat tctgtaacca
     1201 aattctttac atctaaaaga atctcactca taagctattt ttaagttgat gttgagtacg
     1261 aaaatacaat aaatattaaa atatacgcta tacagacaat aagtccatac cttttaattt
     1321 tagaataatt tgtgctgtag ttaaaacatc tttttcgcaa taggttttta tacgttctaa
     1381 atctttttct tgccaataaa ctctgcccac atcttttcca gaaatgtcat ctttgggaga
     1441 tggaataccc aaactataag ctaataattt taaagaaata taatttttat aatcaccaaa
     1501 tttccaaagc tccattgtat ctattgagcc attttcccaa ggtttcttag tttgcagttg
     1561 agccaaaaac ggatgcaact taaatccttt aattaaactt ctgcgacaga tatagggaat
     1621 atcaaactct ttaatattat gaccacaaaa attaatcctt gaattttgtt taaaggattt
     1681 atctaaaaag agataaaaat cctttaaaat tactgattca tcatcgccat aaaatgatat
     1741 tttattatat tccaaggcat tattgttttt agtaactaat ccaacagaaa tacagataat
     1801 cttaccaaat tctgcataga ttccagcatt attaaaataa aaatcttcaa tattttcagt
     1861 ttctaatcta taccttccac gtttttcttc ccaaagtaat tgttcatatt ctggcacctt
     1921 ttgtagattt tcataagctg gcgtagtttc tatatcaata aaaattaagt cagaaatatt
     1981 tgattgttgt agcataattt gaggtttaaa caattataac actaaataga tacccaattt
     2041 acaatattat ttttactttt gtataaaata aaagtaacga tggaggagca aaaacaagat
     2101 aataaaagct accatttctt atatattgct ttaattgcac tattggttgg tggattgata
     2161 tttacaagtt taaaattaaa aaaacaaaaa gagacaattg ttgtaacaga aaatcaaaga
     2221 gatgaagttg ccgcattaag atctgagtta gaagtaaaat ataattcagc aatagtagat
     2281 attgaaaatt ataaaagtga aaatttaggt ttagatagta ttcttagatt aaaagaatta
     2341 gatttaaatt caaaaaaaca aaaaattgaa acattactag ctcaatcttc aaatgataaa
     2401 aatgccttat taaaagcaca gtctctaatt aaagaatttg aagccgaaaa gattgctttt
     2461 agaaatacaa ttgattcgct aattcgagtt aatagggatt taggaataaa atcagatagt
     2521 ttagcgtccg aattaatctt atctatagaa gaaaaaaata aattaaacga agaaaaagca
     2581 aatttagagg aagaacgtaa taaaatgtta gataaaatag ataaagcatc aatattaagt
     2641 atcgcaaata ttgaagcaac acctattcgt atcgtaaaga agcaaaaaga agaaattgtc
     2701 aaaaaagcaa aagatgctga aaaattgaga ctttgctttg atttattaca aaataaaatt
     2761 gcacccaaag gtccaacaga attaattatt agaataattg acccaacagg ttctacaatt
     2821 cagattcaaa gtttaggtag tggttcaatt attgaaaata ctactggaaa ttcaattcca
     2881 tttacttatt ccatttctcc tagttatgac aatgaaaaca aacaagtttg ttcttattgg
     2941 aaacaaaatt attcattccc tgctggtaat tattcagtag aaatttatca aaaaggttta
     3001 ttaattggtc aagctgtatt taatttaaaa taaaaatatt tctatctttt aataaaaaga
     3061 ggctgtccaa tacttttcgg acagcctctt tattttaaat attaacaatc tgcaattatt
     3121 cgcctgctaa gtagatatct actcttctgt ttttagcttt gtctgcttcg ttatcttgct
     3181 tgtctgtaac ccagctattt tcgccataag gtaacaataa aattctctga gagataccca
     3241 caccttctaa atatttctta acagcatcgc ttctttttgc agatagtgct aagttataat
     3301 ctgtagaacc ttcttgagat gcatttcctg ccaaataaat aaagtaattt cctttcatac
     3361 ctgataattt agatttgaat gcatctaaac ttgcctttgc ttctgctgtt aattcagctt
     3421 tatcattttc aaaatagata gaaccaaatt tcttaaacac aatattatct agtctgtttg
     3481 caatttcttt aatattatct tcctgtgcat ttaatttctc aaatgcttct ttaatagaat
     3541 tgtccgcatt tttcacttga tctgctaaat cattgccttt atccattgct tcaattgctt
     3601 ttgaaccaac ttcatctgcc ttctgattta attttgcaat atcgtcttct actttcttct
     3661 tgtttctttc atattcagac ttatctactt tcttagctaa attgcactga atcgtaactt
     3721 cgtgtgaata tccaaaatta gttcttacat ctttcattgg catctcaaat gaatagaata
     3781 aatttactcg ctctttaatt ccagctcctg ctgtaacatg gaagcctgcc gaattcgttt
     3841 ttataaatcc accatatcct cctcctgttc tataacctgc tcctaaccaa actaattgtt
     3901 tatatccaaa cattacattt aagtcaattt gtggatctat agatttatac ttcctaaaca
     3961 ttaatgatgg tgttatcatc caagtctttt tgtcctttcc tccaaatctc gcttcataac
     4021 ttgcaagtac taaatattct ctttcataat gaaacatact tgtttcatta tccattccat
     4081 ttcctctaaa cttagcttta ccatttaaca attgaggcac tgaaaaccca acattcaaac
     4141 ctttccaatg ataatttata cccaaagaaa ggtcaaattt tgttgctctt gaattattcg
     4201 ttactgctgc attatcatta aaaattccat ctgttgtaat tgcatctgta aaattaaact
     4261 tttgagatat aattcctgct tgaataccaa aattcaaacc atggttcttt tctttattaa
     4321 atggcaatct atagttataa actcatttaa acttttaaca agattaaagc aaaaggtaaa
     4381 taatgccaat ttaaccaatg gttatccctt ttatcaaatc ggattaacag ggttttgaaa
     4441 ctgtctatcc aagcaaaaca tctttcattt acgaatctgg ttttgtatat ttcattattg
     4501 taaaaacgct ttttacctag tttcttatat tttctttgat atttggaatt atatttctac
     4561 gcattatagc tcgtctaagt ctcttgctat caaagctttt atctgcattt aggatgctgt
     4621 tttctacaca aatatttttg tgatttagtt ctcttatcat agtcctaaat tgtggaacta
     4681 cacgatataa atcgttatga tttccttcta aaatatctcc tattgcaata ggtattcctt
     4741 tgccatcggt catgattaaa acattagagg tctttccttt tttacgatgc tgatacttta
     4801 cactttcagc tgcttttttt tacgaaacta tgtgttccat caaggttcaa tttttctgtg
     4861 tctaacttgt ctctttggat ttctaaataa gtatcaaaca tttgtttgaa aacaccttgt
     4921 ttgttccatt ttgagtagaa ataatagact gtctgccaca aaatggaggc ttaatgcact
     4981 caatatcaac aaataataaa ttctattgac aacctgtttt cagcttatga acaatacatt
     5041 taaaaatatc accttgattg aaaccacttg aaaatgctct tttgtttttc gaaataaagg
     5101 gcaaaacaca gttttctaat tctacttttg ttatctttgc gagtgaacaa ttcatatctt
     5161 tgtaatttgt tgatttgtaa cccaaaatta catttttttt gaattgctct tttaatataa
     5221 aaagtttaaa tccgtttatt ataaatagac tatttcaacc gcttcattac tcacttatat
     5281 tagtgattca attattaatc aaattatatg ggataaaaaa atacaaaaat ctagctattt
     5341 atgaaatcga caattaatac gacgtttact ataatcagaa attaaactaa ttacaataaa
     5401 catcgaaaaa aaaccataat aaaaaattac tctgatttat cttttttggc taaattcatt
     5461 ctaaatccaa catataaatt ccaacccatt gctgatcccc atatcattga ggcatcaaac
     5521 ttatcagaat aaggtgtgct tgcgcccata attaatggat tttgcatagt attcaacata
     5581 ttttcagcac caacataaat attcatcaaa ccgtccttaa aaattttatt gatttgtgta
     5641 ttcatcatcc aataactagg tgtttttgaa gaagatacaa tatcatttgt tttatcatta
     5701 aaatgttcag gtaaacgttt tgctccaatc cattgaacag tataatcaaa tttccattgt
     5761 tttttagttg tataggctaa attcataaac atacgatgtg ctgcaactaa tggccttttt
     5821 agtaaattat tttgataagt tgttttcaca tcataaaaac gataagccac tctgacatca
     5881 aaattacgaa ctggctgata atcgacttgc atttgtaagc tatgagaata tgatttacca
     5941 tctaagttat aaaaactgac tctattatag ttttcaaaat ctacaacaac ttgattggta
     6001 aagtctgtaa aataataatc aatattgacc gagccatctc tataattcaa caagaatttt
     6061 ttagtcagat ttacacccat attccatgct acttcaggtt taaatggata tttagcaaag
     6121 ctactagttt gaattaattc attattaaaa tagaaatctc tattacttgc cattaagcca
     6181 gtattttctg ctaacatatt tgcagttctt tgagctcttc caatcgatgc gcgaatggct
     6241 aatttttcat ttggtgcata tctaaaatgc aatctaggcg ttgcaaacac accaaataaa
     6301 ttattataat caccacgcaa gcctgcaact atattaattt tatctaacca cttaaaatta
     6361 tattcagcaa aaactcctgg cactatttca tttctctgaa aatatttatt cttataattt
     6421 tccttatatc tatctgctaa aagacttcca cctactttta ttacatgatt agagtttttt
     6481 attatcgttt ggaaaatata gttagcataa taactttctt gttttgcagt atatgttgtt
     6541 gctccgaaat tattagcttg atcatgataa attgcagata actgtaaacc catactttga
     6601 tatggtttgt ctatataaac ttttccaatt tttgcccaag tttcaattcg atttaatgta
     6661 ttttgatagc cccaaggatt tgtacctgaa agttgttcca ttcctctttt ataatctttt
     6721 tgaccaccag ttatgttcaa attcacaact ttaaagccac cttgaatatc ccaacctttt
     6781 tcaccaaaat aaaaccagcg attagaccct acaaaagtat atcctaatgg attatctaag
     6841 aatttatctt tattcatatc tactttcaac cattctcctt taccgtgcaa caaaacactt
     6901 gtcgataatt tcttattgat aacttgattc caaattaagt ttccctctga gcgaccttgc
     6961 gtactttggt atccgtttag aaccagcaat ggagatttct catcaaaagg cttgagccat
     7021 tctacattta tctgtcctgc tgtgccttca aatccattga ctacgcttcc tgcaccttta
     7081 ctcaactgca tactttctac aaacgttcca ggtgtaaaag ttaaaccagt aattgctgct
     7141 aatcctctaa tatctggaac attttcacgt gtaaaagaag tatatgttcc tgccaaacct
     7201 aacatttgaa tttgcttata tccactcact gcatctgtaa atcctacatc aaccgatggt
     7261 gtggtttcaa aactttcact caaattacaa caagcagctt tcatcaactc tccaattcca
     7321 atattttcta ctttcatagt gctataagta ctgactgttg ttgttgcttt ttgtcccaca
     7381 accgtaacgc catctaaaga ttttgttgct tcgtttaaaa agatttgcaa atgctgaata
     7441 tcattagaaa attgaatgat ttttgtttca tatccaagat aacttatttc tagtagtgtt
     7501 actgtatctg gcacttcaat catgaaatga ccttccatat cggtaacctg attaaatttt
     7561 tgatttaatg aactaacata agctccaata actggctctt tagattttgc atctaaaatc
     7621 attccgtgaa tagtcttatt ggctacacta tttaccgaat ctgcctcctg tgcaaaggca
     7681 gatattatcg gcataagcat agctaaagct aaacttataa aaatcttatt cataaagaaa
     7741 attaatgcgg atgaacttcc ttataagcac agcagtctgg aagcttttta taattatcat
     7801 tcgtcgcttt tactgtacca gcattgtgtc cagtctttgc aatat
//
LOCUS       DAOQCW010000114         7809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_3669_length_7809_cov_5.377483, whole genome shotgun sequence.
ACCESSION   DAOQCW010000114 DAOQCW010000000
VERSION     DAOQCW010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7809
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3669_length_7809_cov_5.377483"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..1081
                     /locus_tag="PLS16_09535"
     CDS             218..1081
                     /locus_tag="PLS16_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61364.1"
                     /translation="MFLELLQSKQINYPSGSALEFYNDEFFVVGDDINYILCLDKAFH
                     ETRKIKLFEFDGIRIPKVDKPDLECATILNDILYILGSGSKMPERGYVFTVNLKDNST
                     EKKSMHSFFSKIESQKIIQEINIEGLANYKDKLLFFNRGNTQESNHLIITSVGIFENS
                     NTNNLSIIPIETGQLNGVNLGISDACIDEQNDLLFVTASAEDTNNAYDDGTILGSVIG
                     IVPNFSTQLKEPCIKINQVIKLGEEHTIFSAQKIEAICITAQSENKYHLSLVADNDDG
                     KSVLFDIKLTI"
     gene            complement(1089..1394)
                     /locus_tag="PLS16_09540"
     CDS             complement(1089..1394)
                     /locus_tag="PLS16_09540"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNJ61365.1"
                     /translation="MEENKNEQAELISNFCKALSNPIRVEILQLIAQNKKMMVQEMIA
                     VIPLAQSTISEHLRILKNNQILKVENIGTKSYYAIKTKTIEKSEKSLKKLLKSILNG"
     gene            complement(1406..2131)
                     /locus_tag="PLS16_09545"
     CDS             complement(1406..2131)
                     /locus_tag="PLS16_09545"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HNJ61366.1"
                     /translation="MKKIYIILLSSFLIFGCTTQQVQDAIGSILGTNDSITESEAAGG
                     LQDALGQGLKVGMGLLSKQDGFLGNDLVKIPWPDQAKPILDVMQKLGMQKQVDNVTTS
                     LNRAAEKASGVAIDVFLNAVKQMTIKDAMNILLGGDGTATAYLKKTTTPMLTEKFRPI
                     ISNSLDQVNATKLWGEAITVYNKVPLVKPVNADLTGFVTEKALDGIFKMVEKEENKIR
                     ANPLARVSDLMKKVFGFADSKKS"
     gene            2192..3436
                     /locus_tag="PLS16_09550"
     CDS             2192..3436
                     /locus_tag="PLS16_09550"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HNJ61367.1"
                     /translation="MKAEILTIGDELLIGNTINTNAAWIAQKLNLIGIDVLHHLTLSD
                     EKIDIINSIENALNTVDIIVITGGLGPTNDDITKYVLTDYFGGKLVFNEAAFQNIERL
                     FAHRKKWINEATKKVAYLPDNCIPIQNKMGTAAGMLFTKDNKTIVSMPGVPYEMKAMM
                     SDDFIPYLQKKYELPTILHKHILTAGVGETEIAEKLESFEHNLSKYLKLAYLPSLGSV
                     KLRLTAKGNTAEKLQKIINDATEEIQSKLNEWIFGYDDETLESVIGKKLQSKNLMLGT
                     AESCTGGMLAHKITSVAGSSAYFKGSIISYANEIKEDILGVQKKTLAKYGAVSEETVS
                     EMLLGALKQLDVNIAVAISGIAGPDGGTKEKPVGTVFIGIANKEKQLIKKFSFYKNRA
                     INIELSSVFALVQLRKFLNEYY"
     gene            complement(3478..3804)
                     /locus_tag="PLS16_09555"
     CDS             complement(3478..3804)
                     /locus_tag="PLS16_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61368.1"
                     /translation="MTKIVLIKAEVILFLDELVTLLVEKEYFSFPKNAEIYVNKLYDA
                     IYDLPSLKHYETPQELIRYGKYYVKIKGSKQTMWYVFFDKMDNRYLVEFITNNHSPQS
                     EYLNKL"
     gene            complement(3776..4015)
                     /locus_tag="PLS16_09560"
     CDS             complement(3776..4015)
                     /locus_tag="PLS16_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61369.1"
                     /translation="MAQTLETLNIESQIASYVSALSDKNKQVVLAVVKSIAEADHEAE
                     FEKKWTKAIPLEKAREHTLNTVKKLFNDKNSLNKS"
     gene            4190..5188
                     /locus_tag="PLS16_09565"
     CDS             4190..5188
                     /locus_tag="PLS16_09565"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HNJ61370.1"
                     /translation="MINIAELYTLLETPKKIVITTHQKPDGDAMGSSLALYNYLAPKG
                     HFVRVVTPTDFPTYFNWMPNCDEVWNYEENPTLSNALVYEADIIFCLDFNDLSRIDPF
                     NKAFEQTQAKIVLIDHHLYPKDFAHFILHDVKACSTCELIYRFLELLEPAYKCSKEVA
                     TCIYTGILTDTGSFSNGATNKKAFEITAQLLDAGLNIIEVQENLNQNGREEKLRFIGN
                     ALNRKMTIREDLGIGYIIVDRKDAYTYNLQNGDTEGLVNYPLSIEKVKVAILIKQEAK
                     IIKLSFRSKGTISVEKICRENFEGGGHRNASGGKSFLSLPETINKIISIFEKEKIV"
     gene            complement(5200..7044)
                     /gene="asnB"
                     /locus_tag="PLS16_09570"
     CDS             complement(5200..7044)
                     /gene="asnB"
                     /locus_tag="PLS16_09570"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNJ61371.1"
                     /translation="MCGILGYISKKSIDKQNFQEANIAIQHRGPDAEGFFYNAENTVA
                     FAHRRLSILDLSSAANQPFTSNCGRYIIIYNGELYNFQDIKNKLQLETKTTSDTEVIV
                     EAFAKIGTKVFHQLNGMFAFAIYDQQQNKIFLCRDRLGIKPLFIYQDATNIVFASELK
                     AIKKFSSLDFSINKQVIPAFLHLGFITQPNTIYQKIQKFPTAHFAEIDTKNIQNKIEF
                     IPYWNLSESINQVQITDFDEAKRQLNALLIESVEKQLISDVPIGTFLSGGIDSSLVTA
                     IASKIKKDKINTFSIGFDYNKFDESQYAKQVSEHLQTNHHPFLVKEKDVLEQLHAIID
                     VYDEPYADSSAFPSMLVSKLAKQHVSVALSGDGGDELFYGYGAHIWSERLNKFYFSTF
                     RKLIYATTQRSDDGRIKRAGTLFNYSNKQHIRSHIFSQEQYLFSENELNDLLVQPDFK
                     FDAFNPKEDKKWSKDQQPIWDINHYLKDDLLVKMDRASMQYSLEVRVPILDNSIVDFS
                     FRIPSSFKIKDKTQKYILKEVLYDYVPKQIFERPKQGFAIPLIRWLKKDWNFLIEKYL
                     NKAVVEQYNLVHYTKVADLKNRFLKNEDYLYNRIWVLILIHWWLEENK"
     gene            complement(7109..>7809)
                     /locus_tag="PLS16_09575"
     CDS             complement(7109..>7809)
                     /locus_tag="PLS16_09575"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNJ61372.1"
                     /translation="HYAAVVGVQRTLQNPVMVLDDIKGIENILSLSKNTGVKRIFYSS
                     SSEVYGEPVTLPQNEYTTPLNSRLPYAVVKNIGEAYCRSYHQEFGLDYTIFRFFNTYG
                     PKQSQDFVMSKFLNAALNNKDITVYGDGLQTRTFCYIDDNLDACLTTFNNDAYKNDVY
                     NIGNDDIITIKQLAETIIELTNSKSKIIHLPPLKEGDMTKRQPDISKMKELLNRDLLP
                     YRDGIKKVINAWKI"
BASE COUNT         2690 a         1166 c         1177 g         2776 t
ORIGIN      
        1 ttgtaagcat aaataaagca agttttcaac tagctaaaag aataaacagt atatttgaaa
       61 tagcaattac actatgtacc aaaattacaa cagtttcgga tataagcgtc tattatatgg
      121 ctttacttca agtgtcattt attaattgat tggtcgttta atatttacat tttttcctat
      181 atttggtaaa ttacttgctt tcttatattt ataatttatg tttttagaac tacttcaatc
      241 aaaacaaatt aactatccat ctggctcggc tttagaattt tataatgacg agttttttgt
      301 ggttggtgat gatatcaatt atattttgtg tttggataaa gcatttcacg aaacaagaaa
      361 aataaaattg tttgaatttg acggcatacg cattccaaaa gtggataagc ctgatttaga
      421 atgtgcaact atcctaaatg atatactata tattttaggc tctggatcaa aaatgccaga
      481 gcgtggttat gtatttactg taaatcttaa agataatagc acagaaaaaa aatcaatgca
      541 ttcatttttt agtaagattg aaagtcaaaa aataattcaa gaaataaata ttgaaggttt
      601 agcaaattat aaagataaat tattgttttt taatcgtggg aatacacaag aatctaatca
      661 tttaatcatc acatcagtag gaatttttga aaattcaaat acaaataatc taagtattat
      721 tcctatagaa actggtcaat taaatggtgt gaatttaggc atttcagatg cttgtattga
      781 tgagcaaaat gatctacttt ttgtcaccgc atcggcagag gatactaaca atgcctatga
      841 tgacggcaca attttaggta gtgtaattgg tatagttcca aatttttcaa ctcaactaaa
      901 agaaccctgt ataaaaataa atcaagtgat aaaattaggc gaagagcaca cgatattttc
      961 agcacaaaaa attgaagcaa tttgtattac ggcacaatct gaaaacaaat atcatctttc
     1021 tttagttgca gataacgatg atggcaaatc tgtattattt gatattaaat tgacgattta
     1081 gcaaattcct atccatttaa aatactcttc aataatttct tcaaactttt ctccgatttc
     1141 tctattgttt ttgttttgat agcataatag gattttgtac ctatattttc aacttttaaa
     1201 atttgattgt ttttcaaaat tcttaaatgc tcagaaattg tcgattgagc tagtggaata
     1261 acagcaatca tttcctgcac catcattttt ttattttgtg ctattaattg gaggatttca
     1321 acacgaattg gattagaaag tgctttgcaa aagttgctaa ttaattccgc ttgttcgttt
     1381 ttattttcct ccattttgaa aatatttaag actttttaga atctgcaaat ccgaatactt
     1441 ttttcattaa atctgaaact cttgccaaag gatttgcacg aattttattt tcttctttct
     1501 ctaccatttt aaaaattcca tctaatgctt tttctgttac aaacccagtt aaatcagcat
     1561 ttactggctt tactaatggc actttattat aaactgttat cgcttcaccc cacaattttg
     1621 tagcgtttac ttggtctaat gaattagaaa taattggtct gaatttttct gtaagcatag
     1681 gagtggttgt tttttttaga taagctgtcg cagttccatc tccacctaac aaaatattca
     1741 tagcatcttt aatcgtcatt tgttttacgg cattcaaaaa aacatcaatt gctacacccg
     1801 atgctttttc tgcggcacga ttcaaagaag tagtaacatt atctacttgt ttttgcatac
     1861 ctaatttttg cattacatca agtattggtt ttgcttgatc tggccatgga atttttacta
     1921 aatcattacc caaaaatcca tcttgttttg ataacaagcc cattccaact tttaatcctt
     1981 gtcctaatgc atcttgtaat ccacctgcgg cttcgctttc tgtaattgaa tcattcgtgc
     2041 ctaatattga accaattgca tcttgaactt gttgggttgt acagccaaaa attaaaaacg
     2101 aactcaacaa aataatgtat atttttttca tacttaaact ttttaaccct aaatttatta
     2161 ataaataata tgccaaaata ataaaaaatc tatgaaagca gaaattttaa ctattggaga
     2221 cgaactatta attggaaata ccattaatac caatgccgct tggattgctc aaaaattaaa
     2281 cctaattgga attgatgttc tacatcacct tacgttatct gatgagaaaa tagacatcat
     2341 taatagtata gaaaatgctt tgaatacagt tgatattatt gtaataacag gtggtttagg
     2401 tccaacaaat gatgatataa ccaagtacgt attaacagat tattttggtg gaaagttggt
     2461 ttttaatgaa gcagcctttc agaatattga acgactattt gcccatagaa aaaaatggat
     2521 aaatgaagcc actaaaaaag tagcttattt gcccgacaat tgtattccta tccaaaataa
     2581 aatgggaact gcggctggaa tgctatttac aaaagacaac aaaactattg tttcaatgcc
     2641 aggtgttcct tatgaaatga aagcgatgat gtcagacgat tttattcctt atcttcaaaa
     2701 aaaatatgaa ctgccaacaa ttttacacaa gcatatttta actgctggtg ttggcgaaac
     2761 agaaattgcc gaaaaattag aatcttttga acataaccta tcaaaatatt tgaaattagc
     2821 ctatttgcca agtctaggta gtgtaaaatt aagattgaca gcaaaaggaa ataccgcaga
     2881 aaaattacaa aaaattatta atgatgctac tgaggaaatt caatctaaat taaatgaatg
     2941 gatttttggc tatgatgatg aaacattaga atctgttatt gggaaaaagc tacaatcaaa
     3001 aaatttaatg cttggtactg cagaaagttg cacaggaggc atgcttgctc acaaaatcac
     3061 atcagttgct ggaagttctg cttattttaa aggtagcatc ataagttatg ccaatgaaat
     3121 aaaggaagat atattaggtg tacaaaagaa aactttagca aaatatggtg cagtaagtga
     3181 ggaaactgtt tcagaaatgc tattgggtgc tttaaaacaa ttagatgtta atattgctgt
     3241 tgcaatatca ggtattgcgg gaccagatgg tggcactaaa gaaaagcctg ttggcactgt
     3301 ctttattggt attgccaata aagaaaaaca acttatcaaa aaattttctt tttataaaaa
     3361 tagagctata aatattgaac taagtagtgt atttgcattg gtgcaactaa gaaagttttt
     3421 gaatgagtat tattagaatt taatacaaaa aatttttaac caaaagaaat ttgacaatta
     3481 caatttgttt aggtattcac tttgtggcga atggttattt gtaataaatt caactaaata
     3541 tcgattatcc attttgtcga aaaacacata ccacatcgtc tgtttgcttc cttttatttt
     3601 tacataatac ttcccatatc ttattaattc ttgaggcgtt tcataatgct ttaaagaagg
     3661 taaatcatag atggcgtcat ataatttatt aacatatatc tcagcatttt ttggaaaact
     3721 aaaatattct ttttctacta ataaggtaac aagttcatct aaaaatagaa ttacttcagc
     3781 ttttattaag actatttttg tcattaaata gttttttaac cgtatttaag gtatgttctc
     3841 ttgctttttc caacggaatt gcttttgtcc attttttttc aaattcagct tcatggtcag
     3901 cttcagcaat agattttacc acagctaaaa ccacttgttt atttttgtca gataaggcac
     3961 taacataact ggctatctga ctttcaatat ttaatgtttc taatgtctgt gccattttgg
     4021 tttgcataat tctttacaaa tatacttgtt ttatttaatt tgtacgaact tgcaatttaa
     4081 tgtaaaattc tttagtcaaa gaagtttagg aaatagcata actcaatctt ttggtatctg
     4141 ataaaatcag ccctttaata aatcgctagt ttttccgtaa ttttgcctta tgataaacat
     4201 tgctgaatta tatactttac tggaaacgcc taaaaaaatt gtaattacaa cacatcaaaa
     4261 accagatggt gatgcaatgg gttcttcatt agctttatat aattacctag caccaaaagg
     4321 tcattttgtt agagtggtta caccgactga ttttcctact tattttaatt ggatgcccaa
     4381 ttgtgatgaa gtttggaatt atgaagaaaa tccaacttta agcaatgctt tagtttatga
     4441 agcagatatc attttttgtt tagattttaa tgatttaagt agaattgatc ctttcaataa
     4501 agcttttgaa cagacgcaag cgaaaatagt attaattgac catcatttat atccaaaaga
     4561 ttttgcacac tttattttgc atgatgtaaa agcctgttca acttgcgaat taatttatag
     4621 atttttagaa ctattagaac cagcatacaa atgttcaaaa gaagttgcta cttgtattta
     4681 tactggaatt ttgactgata ctggaagttt ttctaatggt gcgacaaaca agaaggcttt
     4741 tgaaataacc gctcaattat tagatgctgg attaaatatt attgaggtac aagaaaattt
     4801 gaaccaaaac ggaagagaag agaagttgcg atttattggt aatgcattaa atcgtaaaat
     4861 gacgataaga gaagatttag gtattggtta tattattgta gatagaaaag atgcctatac
     4921 ttacaattta caaaatggcg atacagaagg tttagtcaat tatccattga gtatagagaa
     4981 agttaaagtt gctattttaa ttaaacaaga agcgaaaatt ataaaactat ctttccgttc
     5041 aaaaggaaca atttctgtag aaaagatatg tcgtgaaaat tttgaaggtg gtggacacag
     5101 aaatgcatca ggtgggaaat cttttctctc attaccagaa actataaata agattatatc
     5161 catatttgag aaggaaaaaa ttgtataaac taactaagct tatttatttt cttctaacca
     5221 ccaatgaata agtattaaga cccaaattct attataaagg tagtcttcat tttttagaaa
     5281 tctatttttt aagtctgcaa ctttagtgta atgcactaag ttatattgct caacaacggc
     5341 tttgtttaaa tatttttcta tcaagaaatt ccagtccttt tttaaccaac gaataagtgg
     5401 aattgcaaag ccttgtttag gtcgctcaaa aatttgttta ggaacataat cgtagagcac
     5461 ttctttcaaa atatactttt gtgttttgtc tttaatttta aaagaagaag gaattctaaa
     5521 tgaaaaatca acaatggagt tatctaaaat aggcactcga acttctaagg aatattgcat
     5581 actggctcta tccattttta ccaataaatc atcttttaaa taatgattga tgtcccaaat
     5641 cggctgttga tctttagacc attttttatc ttcttttgga ttaaacgcat caaatttgaa
     5701 atcaggttga acaagtaaat cgtttaattc attttcagaa aaaagatact gttcttgaga
     5761 gaaaatatga cttctaatat gttgcttatt actataatta aagagtgttc ctgctctttt
     5821 tattctacca tcatcactgc gttgggttgt tgcataaatt aattttctaa aagtagagaa
     5881 atagaattta ttcaaacgct ccgaccaaat atgagcgcca tagccataaa acaactcatc
     5941 gccgccatcg ccagaaagtg caacacttac atgctgtttt gctaatttag acaccaacat
     6001 agatgggaat gctgaagaat ctgcataagg ctcatcatag acatcaataa tcgcatgcaa
     6061 ttgctctaaa acatcttttt cctttactaa aaaaggatgg tgattagttt gtagatgttc
     6121 agaaacttgt tttgcatatt ggctttcatc aaatttatta taatcaaaac ctatactaaa
     6181 cgtattaatt ttatcctttt taattttgga agcaatggca gtaactaagc tagaatcgat
     6241 gcctccactt aaaaaagttc caattggcac atcagaaatc aattgttttt ctacgctttc
     6301 aatcaataag gcattgagtt gtcttttggc ttcatcaaag tctgtaattt gaacttgatt
     6361 aatgctttcg ctcaaattcc aatatggaat aaattctatt ttattttgaa tgtttttggt
     6421 atctatctca gcaaaatgag ctgtcggaaa tttttgtatc ttttgataaa tagtattggg
     6481 ttgagtaata aaacctaagt gcaaaaacgc tggaataact tgtttgttga tgctaaaatc
     6541 taaactagaa aattttttaa ttgcctttaa ttctgatgca aatacaatat tagttgcatc
     6601 ttgataaata aacaaaggtt tgattcctaa tctatctctg cataaaaata ttttgttttg
     6661 ctgttggtca taaatagcaa aagcaaacat gccattaagt tgatgaaaaa cttttgtacc
     6721 tatttttgca aatgcttcta caataacttc tgtatcagaa gtcgtttttg tttctaattg
     6781 tagtttgttt tttatgtctt gaaaattgta taactcgcca ttataaataa taatgtatct
     6841 accgcaattt gaagtaaatg gttgattggc agcagaactt aaatctaaaa tacttaaacg
     6901 gcgatgagca aaagccactg tattttcagc attataaaaa aaaccttcag catctggtcc
     6961 acggtgttgt atggcaatat ttgcctcttg aaaattttgt ttatcaatag attttttgct
     7021 gatatatcca agaatgccgc acattggttg atatactaaa ttgtagaatt ataaattttt
     7081 gtaattacag aattattact ttggtacatt atatcttcca agcattaatt acttttttaa
     7141 ttccatctct ataaggtaat aaatctctgt tgagcaactc tttcatttta gaaatatcag
     7201 gttgtctctt tgtcatatcg ccttctttta atggtggcag atgtataatt tttgatttag
     7261 aatttgtaag ctcaataatt gtttctgcta attgtttaat cgtaataata tcatcatttc
     7321 caatattata aacgtcattc ttataagcat cgttattaaa tgttgtcaaa caggcatcta
     7381 aattatcatc gatataacag aaagttcgag tttgtaatcc atctccataa actgtaatat
     7441 ccttattgtt taaggcagca tttaaaaatt tagacattac aaaatcttga ctttgctttg
     7501 ggccataagt attgaaaaat ctaaagatag tataatccaa gccaaattcc tgatgataag
     7561 aacggcagta ggcttctcca atgtttttta ccactgcata aggcaaacga gaattaagag
     7621 gtgtagtata ttcattttgt ggaagcgtaa ccggctcacc ataaacttct gatgatgatg
     7681 aataaaatat acgtttaacg ccagtatttt tagataaaga caaaatattt tcaatgcctt
     7741 ttatgtcatc taataccatt accggatttt gcaaagtacg ctgcacacca acaactgcag
     7801 cataatgaa
//
LOCUS       DAOQCW010000115         7686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_3764_length_7686_cov_4.955838, whole genome shotgun sequence.
ACCESSION   DAOQCW010000115 DAOQCW010000000
VERSION     DAOQCW010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7686
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3764_length_7686_cov_4.955838"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /gene="nrfD"
                     /locus_tag="PLS16_09580"
     CDS             <1..1102
                     /gene="nrfD"
                     /locus_tag="PLS16_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNJ61373.1"
                     /translation="EAMTIFAVVCAGLFPVFHMGRVWDAMFIFPYPNTRGPLWVNFTS
                     PLLWDVFAISTYFTVSLLFWYTGLVPDFATIRDRSKTKGRWTFYNLLSFGWIGSVKHW
                     ERWESLSLILAGLSTPLVLSVHTIVSFDFATSVIPGWHTTILPPYFVAGAIFSGFAMV
                     LTLMIVARYIMKLEDYITLAHIESMNKILLLTGTMVGIAYLTELFTAWYSSNQYEQHQ
                     FATRAFGSTYAWAYWIMMSCNLISPQIFWFRKFRRSIKVTFVMSILVNIGMWFERFVI
                     IVISLHRDYLPSSWLYYSPTWIEIGIFVGTIGIFMTLFLAFCRFFPVVAIAEVKSILK
                     RNGSQAKAHQLEEVADQEVYKSVNKEELLQYK"
     gene            1130..1651
                     /locus_tag="PLS16_09585"
     CDS             1130..1651
                     /locus_tag="PLS16_09585"
                     /inference="COORDINATES: protein motif:HMM:NF023249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HNJ61374.1"
                     /translation="MSNTKKYLVGMFDDDDVLLHAIKNIRKEGFKITDCFTPFPVHGI
                     ESAMSIEPTRLHTAGFMFGATGMSVALGVITFINAFDYPTIFGGKPYWALMAYVPIVF
                     EHTVLYSAVGMVIAFYYLCKLYPGKKPIIIDERTTDHLFALTFEMDGSQDTAAISDLL
                     KKEGAIEVYQKEV"
     gene            1661..2260
                     /locus_tag="PLS16_09590"
     CDS             1661..2260
                     /locus_tag="PLS16_09590"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNJ61375.1"
                     /translation="MKQLSYITILSFIILTSACIRNSKNPGREYMPDMVHSTAYEAGS
                     VNPIFKDGKTNQLPPKGSVAQGKYIYPYDNTIEGYEASVANKNPFTFTKDEVDNQGQH
                     LYKINCAICHGDKGDGQGHLVEIGKFPPPPTYLSGVLLTLPEGKRYHTVMYGKGLMGS
                     YASQLDHRERWLVLEYVKTLQGDAGASAPATDSTSTAAK"
     gene            2302..3621
                     /locus_tag="PLS16_09595"
     CDS             2302..3621
                     /locus_tag="PLS16_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61376.1"
                     /translation="MNTYTFESGTKSKILITFVIGVLILLLGIGLNMGKGDKTSNHNV
                     THQAEAKDPGHDAHGGHNADAHGHDSHEQPITLKAKLLANFYSLFIFAFYIALAALFF
                     ISATTITWGGWHIQIQKIPLAMTRNIFVFLFILFLCFFFFKHDLFEWTHEYLYDKNAE
                     TYDPILAFKRDYLNMPRFWAFFIIQVLAAGGMSFLWWRNMDKQDTNPSLKLFSQSRVY
                     SAVTIVLIAFVISTFATWDWTMSVQPHWYSTMWPWYTMASGAVTMFSIVILIILHLKS
                     KGLLARVNENHMHDIGILMFAVSIFWTYLYFDQYMLIWYGNIPEETIFFLKRRTWDTY
                     GFIFHLTVVVNFLLPLLILMTRKAKRTPKVLAIMAVIIILGHWMDFYLMVGPPLVPSG
                     GFGLISLGALLIVGSLFTFVTLASLAKVKDLESSTHPYVKESYNHHI"
     gene            4211..5683
                     /locus_tag="PLS16_09600"
     CDS             4211..5683
                     /locus_tag="PLS16_09600"
                     /inference="COORDINATES: protein motif:HMM:NF012344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNJ61377.1"
                     /translation="MFTAVLILLIIALLFFVVLQISKTSDLIDAIKKENNKESHSKSA
                     LYFSLIGFGVLIYTIISWITESKKFLPVSASEEGVAIDNLMNFILVLTGIVFILTHIL
                     LFYFVYKYHYRKDRKADFYPHNSKLEIIWTTVPAIVMIGIVLFSLSSWSKIFSKQDEG
                     TLAIEVTGKQFAWVVRYPGKDGKLGVRDFTLVNSDNELGVNWNNKNSHDDMLVDEIVL
                     PVNKPVIVHIGALDVIHSFYLPEFRVMMDAVPGVPTKMAFKPTITTEEMRKKLENPDF
                     NYVLACNQLCGSGHYNMQKRVRVVTEDEYKQWLREQKSYYRTVVKPALDDKAAQDTAV
                     VTTNSGNFENTLTTGFSLVGAITNGVENKLIDFLNSNQAVSKDLWFSFDRLLFATGKS
                     TLLPSSQEQLKNIAEILKAYPNVEIKLGGYTDNIGKPEDNLKLSTDRAKSVLNELSKL
                     GVDVKRLSSEGYGQEHSVASNDTDAGRQQNRRIDIRVTKK"
     gene            5710..7497
                     /locus_tag="PLS16_09605"
     CDS             5710..7497
                     /locus_tag="PLS16_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNJ61378.1"
                     /translation="MVTSAIENNIDGQAHHHTETFISKYIFSLDHKIISRQFLFAGMA
                     WAVLGGLLSVLFRTQLAWPNETFPVLETLFSTWYTDGILKPEAYNAMITMHGTIMIFF
                     VLTASLTGTFANLLVPFQIGARDMASPLLNALSFWIFILASVFMLASLFAPDGAPSAG
                     WTVYPPLSALPQTSPGSGLGMTLWLIAIALFIISTLMGGLNYISTILNMRTKGMTMYR
                     MPLSVWALFFTAIVGLLAFPVLLSAAVLLIFDRGLGTSFFLSNIVVGGSPLPYSGGSP
                     ILFQHLFWFLGHPEVYIIILPAMGIVSEVLAIHSRKPVFGYKAMIFSLLGITIMSFLV
                     WAHHMFVSGMNPFLGGVFTVFTLLIAIPSSIKVFNWLTTLWRGNFHINPASIFAVGFV
                     SLFISGGLTGIWLGSSTVDIRLHDTYFVIAHFHLVMGVAAFFGMFAGVYHWFPRMFGR
                     MMNNTMGYIHFGITFVGAYLIFWPMHFMGLSGVPRRYYHFNEFDAFSQYANMNVIITV
                     VTIIVFITQFIFIFNFFHSMYFGKKAPQNPWNATTLEWTSPVEGIHGNWEGDLPVVHR
                     WPYDYSKPGADEDFIAQTTPLKEGEIESH"
BASE COUNT         2435 a         1023 c         1322 g         2906 t
ORIGIN      
        1 agaagcgatg actatctttg ctgtagtttg tgctggtttg tttccagttt tccacatggg
       61 acgtgtttgg gatgcaatgt tcattttccc ttatcctaat acgagaggtc cactttgggt
      121 aaacttcaca tcaccattac tttgggacgt atttgcgatt tcaacttatt ttacagtatc
      181 attattgttt tggtacacag gtttagttcc agattttgca actatcagag accgttctaa
      241 aactaaaggt cgttggactt tctataatct attaagtttt ggttggatag gtagtgtaaa
      301 gcattgggaa agatgggagt ctttatcatt aatattagct ggtttatcta ctccattagt
      361 attatcagtt cacaccattg tatcttttga ctttgctact tcagtaattc caggttggca
      421 cactacaatc ttaccgccat atttcgttgc gggtgcaata ttctctggtt ttgcgatggt
      481 attaacatta atgattgttg ctcgatatat tatgaaatta gaagattata tcactttagc
      541 tcatattgaa tcaatgaaca aaattttgtt attgactggt acgatggttg gtatagctta
      601 tttaacggag ttatttacag cttggtattc ttcaaatcaa tacgaacaac atcaatttgc
      661 tactagagca tttggttcta cttatgcttg ggcatattgg attatgatgt cttgcaactt
      721 aatttctcca caaatctttt ggtttagaaa gtttagaaga agtattaaag taacttttgt
      781 aatgtctata ttggtaaata taggtatgtg gtttgaacgt tttgtgatta tcgtaatttc
      841 attacataga gattatttac catctagctg gttatactat tctccaactt ggattgagat
      901 aggaattttt gttggtacga ttggtatttt tatgacattg ttcttagcat tttgtagatt
      961 tttcccagtt gtagcaattg ctgaagttaa atccatcttg aaaagaaatg gtagtcaagc
     1021 taaagctcat cagttagaag aagtagctga ccaagaagtt tataaatcag taaacaaaga
     1081 agaattatta caatataaat aataactaac acatcttaga ataaaagata tgagcaatac
     1141 aaaaaaatat ttagtgggaa tgtttgatga tgatgatgtt ttattacatg ccattaaaaa
     1201 tattcgcaaa gaaggattta aaataacaga ttgttttaca cctttcccag ttcatggtat
     1261 tgaaagtgca atgagcattg aaccgacaag attacataca gcaggtttta tgtttggagc
     1321 aacaggtatg tcagttgctt taggtgttat aacttttatt aatgcatttg attatcctac
     1381 tatttttggt ggtaaaccct attgggcttt aatggcttat gttcctattg tatttgaaca
     1441 tactgtttta tattctgctg taggtatggt gattgcattt tattatttat gtaaattata
     1501 tccaggaaaa aaacctatta ttattgacga aagaactact gaccacttat ttgctttgac
     1561 atttgaaatg gatggaagtc aagatacggc tgctatttct gatttattga aaaaagaggg
     1621 tgctattgag gtttatcaaa aagaagttta atattataac atgaaacaat tatcatatat
     1681 tacaatttta tcatttatta tactgactag tgcttgtatt agaaattcta agaatcctgg
     1741 tagagagtat atgcctgata tggttcatag tactgcttat gaagctggaa gtgtgaatcc
     1801 tatatttaaa gatggcaaaa caaatcaatt acctcctaaa ggtagtgttg ctcaaggtaa
     1861 atatatttac ccttacgata acactattga agggtatgaa gcatctgtag caaataaaaa
     1921 tccttttact tttactaaag atgaagtaga taatcaagga caacacttat acaaaattaa
     1981 ttgtgctatt tgtcatggag ataaaggcga tggtcaagga catttagttg aaattggaaa
     2041 atttccacca cctccgacgt atttatctgg tgtactttta actttaccag aaggtaaaag
     2101 atatcacact gtaatgtatg gaaaaggttt gatgggttct tatgcatctc agttagatca
     2161 cagagaaaga tggttagttt tagaatatgt taaaacttta caaggtgatg ctggtgcatc
     2221 agcacctgct acagactcaa caagtactgc agcaaaataa ttaactgatt aagtttaaat
     2281 agaaaatata ataagatact aatgaataca tatacttttg aatcgggtac aaagtctaaa
     2341 atcctaatta catttgtaat tggagtatta atccttttat taggtattgg attaaatatg
     2401 ggcaaaggag ataaaacttc aaatcataat gttactcatc aagctgaagc aaaagatcct
     2461 ggacatgatg ctcatggcgg acataatgca gatgctcatg gacatgatag tcacgaacag
     2521 ccaattactt tgaaagctaa attattggct aacttctact ctttattcat atttgcattc
     2581 tatattgctt tagcggcgct attctttatc tcagctacga caataacatg gggtggatgg
     2641 catatacaaa tacagaagat tccattagca atgacaagga atatttttgt atttcttttt
     2701 attttgttct tatgtttttt ctttttcaaa catgatttgt ttgaatggac gcatgaatat
     2761 ttatatgaca agaatgcaga aacctatgat cctattttag catttaaaag agattacttg
     2821 aatatgccaa gattttgggc attcttcatc atccaagtat tagcagctgg tggtatgtct
     2881 tttttatggt ggagaaatat ggataaacaa gatacaaatc catctttaaa attattttct
     2941 caaagtagag tttattctgc ggttacaatt gttttgattg cgtttgttat ttctactttt
     3001 gcaacttggg actggactat gtcagtacaa ccacattggt atagtactat gtggccttgg
     3061 tacacaatgg ctagtggtgc tgtaactatg ttctctattg taattttaat tattttacat
     3121 ttaaaatcta aagggttatt agctagagtt aatgaaaacc acatgcatga tatcgggata
     3181 ttaatgtttg cagtaagtat tttctggaca tatttatatt tcgaccaata tatgttaata
     3241 tggtatggta acattccaga agaaacaatt ttctttttaa agagaagaac ttgggatact
     3301 tatggtttta ttttccactt aactgttgtt gtgaatttct tattaccatt attaatatta
     3361 atgacaagaa aggctaaaag aactcctaag gttcttgcga taatggctgt cattattatt
     3421 ttaggacact ggatggattt ctatttaatg gttggtcctc cattagtacc aagcggtggt
     3481 tttggattaa ttagtttagg agcattatta atagtaggta gtttgtttac gtttgttact
     3541 ctagcttcac ttgcaaaagt taaagactta gaatcttcaa ctcatcctta tgtgaaggaa
     3601 agttataatc atcatattta ataattaata tcataaacaa atagattatg ccttctttat
     3661 ttttaatcct tttacttgtt gcagcgataa tttttatcgt ttttcaaatc aagaaaatat
     3721 caaatttgat aaatgttttt aaagaactta agcaacaaaa tttagcttaa tttttaagga
     3781 tatcagattc atataagact ttggttaaat atctcccttt atttttttgg gagattgatt
     3841 taaccaaagt ttttttattt tattcttttc tctaattaca atgaattata atagttttct
     3901 ccttgattta cttatttttc ataatatata gtatctatat tttatttaca ttagaaataa
     3961 ttttcaaaaa ttattgtaaa taaataagtt atattttgag tagaatttta ttcaaaataa
     4021 ttaattattt tttcaccttt tttctttttt atttaatttt attttttacc tttggcttgt
     4081 gaatatacgc atagtatatt tgttaattaa gattaataat atttaatttc cctttttgtt
     4141 aatttttaat ttacattaca aaatataaaa tgtttatcat cataaataaa acaatttaaa
     4201 ataacaaaat atgtttacag cagttctaat ccttttaatt attgctttac ttttctttgt
     4261 tgtgcttcaa ataagcaaaa cttcagattt aatagatgct attaaaaaag aaaacaataa
     4321 agaaagccat tcaaaaagtg ctttgtattt ttcattaata ggttttggtg ttctgattta
     4381 tactattata agttggatta ctgaatctaa aaagtttctt ccggtttctg cttctgaaga
     4441 gggagttgca attgataatt taatgaactt tattctagtt ttaactggta tcgtttttat
     4501 tttaacgcat atattgttgt tttattttgt ttataaatat cattatagaa aagatcgtaa
     4561 agcagatttt tatcctcata atagtaaatt agaaattatt tggacaacag tacctgctat
     4621 tgttatgatt ggtattgttt tatttagttt aagtagctgg agtaaaatat tttctaaaca
     4681 agatgaagga actttagcta tagaggttac aggtaaacaa tttgcatggg tagttagata
     4741 tcctggtaaa gatggaaagt taggtgtaag agattttact ttggttaatt ctgataatga
     4801 attaggtgtt aattggaaca ataagaacag tcatgatgat atgttagtgg acgaaattgt
     4861 tttgccagtc aataaacctg ttattgttca tattggtgca ttagatgtaa ttcatagttt
     4921 ttatttacct gaatttagag tgatgatgga tgctgttcca ggtgttccaa ccaaaatggc
     4981 ttttaaacct acaattacga ctgaagaaat gcgtaaaaaa ttagagaatc ctgattttaa
     5041 ttatgtatta gcatgtaatc aattatgtgg tagtggacac tacaatatgc aaaaaagagt
     5101 tagagtagta acagaagatg aatacaaaca atggttaaga gagcaaaaat cttattatag
     5161 aacagtagtt aaaccagctt tagatgacaa agctgctcaa gatactgctg ttgttacaac
     5221 taattctgga aattttgaaa atacattgac tactggattt agtttagtag gagctatcac
     5281 taatggagta gaaaataaat taattgattt cttgaatagt aaccaagcag tttctaaaga
     5341 cttatggttt tcttttgata gattgttatt tgctactgga aaatctactt tattaccaag
     5401 ttctcaagaa caattaaaaa atattgctga aatattaaaa gcttatccta atgttgagat
     5461 taaattaggt ggctatactg ataatattgg aaaacctgaa gataatttaa aactatcaac
     5521 ggatagggca aaatcagtat tgaatgaatt atcaaaacta ggtgttgatg ttaaaagatt
     5581 atcttctgaa ggatatggac aagaacattc tgttgcatct aacgatacag atgctggaag
     5641 acaacaaaat agaagaatag atattagagt tactaaaaaa taataaatta aaattaaaag
     5701 ttatataaaa tggttacttc tgcaatagaa aacaatatag atggacaagc acatcaccat
     5761 acagaaacct ttatctctaa atatattttt agtttagatc ataaaataat ttcacgccaa
     5821 ttcctttttg ctggtatggc ttgggcagtt ttaggtggat tactttcagt gttatttaga
     5881 acacaattag cttggcctaa cgaaacattt cctgtattgg aaacgctttt tagtacttgg
     5941 tacactgatg gaatattaaa gcctgaagct tataatgcaa tgattaccat gcacggtaca
     6001 ataatgattt tctttgtact tactgcaagt ttaacaggta catttgcaaa tttattagta
     6061 ccttttcaaa ttggagctag agatatggct tctccattat taaacgcact ttcattttgg
     6121 attttcattt tggctagtgt ctttatgttg gcttctttgt ttgcaccaga tggtgctcct
     6181 tcagctggtt ggacagttta tcctccatta agtgcattac ctcaaacttc tcctggttca
     6241 ggattaggaa tgacactttg gttaatcgcc attgcactat ttattatttc tactttaatg
     6301 ggtgggttga attatatttc aactatttta aatatgcgta ctaaaggtat gactatgtat
     6361 agaatgccat taagtgtttg ggctttattt tttactgcaa tagttggtct attagctttt
     6421 ccagtattat tatcagctgc tgttttatta atatttgaca gaggattagg tactagtttc
     6481 tttttatcaa acatagttgt tggtggaagt ccattaccat attctggtgg tagtccaatt
     6541 ttgttccaac atttattttg gttcttagga caccctgaag tatatattat tattttacca
     6601 gcaatgggta ttgtgtctga agttttagcg attcattcga gaaagccggt atttggttat
     6661 aaagctatga ttttctcatt attgggaatt acgattatga gtttcttggt ttgggctcac
     6721 cacatgttcg tttctggaat gaacccattt ttaggtggcg tttttacagt ttttacctta
     6781 ttgattgcga ttccatcgtc tataaaagta tttaactggc ttacaacctt atggagaggt
     6841 aattttcata tcaacccagc ctctattttt gctgtcggtt ttgtgtcttt atttatttca
     6901 ggaggtttaa caggtatttg gttaggtagt tctacagtgg atatccgttt acatgatact
     6961 tattttgtta ttgctcactt ccacttggta atgggtgttg ctgctttctt tggaatgttt
     7021 gctggtgttt atcattggtt tccaagaatg tttggtagaa tgatgaataa tactatgggt
     7081 tatattcatt tcggtattac ctttgttggt gcctatttaa tcttctggcc tatgcacttt
     7141 atgggtttat ctggcgttcc aagaagatat tatcatttta atgaatttga tgcattttct
     7201 cagtatgcta atatgaatgt gataattaca gttgtaacaa ttattgtttt tatcactcag
     7261 tttatattta tttttaattt cttccatagt atgtactttg gtaagaaagc acctcaaaac
     7321 ccatggaatg ctactacatt agaatggacg tctcctgtgg aaggaattca tggaaactgg
     7381 gaaggagatt tgcctgttgt tcacagatgg ccttatgatt atagtaaacc aggtgctgat
     7441 gaagatttta ttgctcaaac tacgccatta aaagaaggag aaatagaaag tcattaatat
     7501 ttttttcaag ttattttgat tactaatcat acatatcaat catcactaag atattcaatt
     7561 aaaaaatgga tagccatatt tagtgcaatt acaaaattga gattgtcttt attggttgtc
     7621 ttttctgctg taatgtctta ttggtttgca aatgttaatc taaatgcaaa aagtttaatt
     7681 ctttta
//
LOCUS       DAOQCW010000116         7665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_3783_length_7665_cov_6.007622, whole genome shotgun sequence.
ACCESSION   DAOQCW010000116 DAOQCW010000000
VERSION     DAOQCW010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7665
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3783_length_7665_cov_6.007622"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..1625)
                     /locus_tag="PLS16_09610"
     CDS             complement(192..1625)
                     /locus_tag="PLS16_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61379.1"
                     /translation="MLSISSTVFAQKISLSTEEKLPGKILDVDILGKNNFGTIIYYYG
                     DNNNQLAILDDKMNTKTRKDISVQSKSIQSVQVLDDKIVVLYVQNSSNLQYLKAKVLN
                     EHLNEVNTLILDSLSIHLDNKNTFYAKTSADRTKLLTFTLVRNKGNFHIQFVTFNQDI
                     ALISRSIFTVTDKDNVSVRSMKINNHGFVSAVFGYQDKWSNDDYDFSSYLVNYYNPTN
                     KTIYEVEIKNTNTVYKKLITETDLDDDKLYILSGFRNTTNKDNLGFYYHILDLKSNQV
                     LVQKYFNLTEDDVKESKTYDFKNWRDKIYTLMPKKIIPRSDGGLLFIVEGEFSYLRQE
                     RSNNYSNFYNPIFSNIPQQVRSTQYNTIGDIMIFSINSDGSLLWKKNIYKSQQSEDDD
                     AIFSSFTLFEAENVLKFIYNEDIFATGNFVEYNINPLGNSKHVSIFNTEKEKLNLIPK
                     KAVQLDANTLMIPSQNKRNIQFLQIAY"
     gene            complement(1716..2957)
                     /locus_tag="PLS16_09615"
     CDS             complement(1716..2957)
                     /locus_tag="PLS16_09615"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ61380.1"
                     /translation="MTTANSVELKQDYWSYVKRQFKKNKRALFSLYIVGVMALIAIFA
                     DVLANEKPIVCKYEGKTYFPIFNQYLVDLKIGKPYAELNNISWIDEDFDFAIRPPIPY
                     SPNTQDILNFGFVSPFGKQDVKSIRWRHWLGTESIGRDVLSALIHGTRIAFMVGLVSM
                     SIASVIGILLGSIAGFFGDNRLKASRATLFMFIITGFFGFFYAFTFGKILLGIIIILV
                     SFILAYFLGFILKPIPFFKKKIAIPVDILIQRLIEVLVSIPGLFLIIAIIAIAKPSIF
                     LVMVVIGLTSWTEIARFIRAELLKVRSLEFIEAAEALGYPKWRILLRHAIPNSVGPVL
                     ITIAFGIAAAILIESFLSFLGLGIPPETLTWGKLLSLAGGGKSELWVAIFPGFAIFLT
                     VTLFNLIGEGLTDALDPRQKK"
     gene            complement(2961..4382)
                     /locus_tag="PLS16_09620"
     CDS             complement(2961..4382)
                     /locus_tag="PLS16_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ61381.1"
                     /translation="MLKYILKRLLIFIPTLFVIALATFYLSVNVPGDPVEQMLNSNND
                     AGSSAGQKASEQAYIDKRHELGLDLPVFYFSISNQAMPKNLYEIPKKFQRQNLNQFIK
                     KYGNWNEINIYYNQVQKFSYLVSDIPSDSTNADILINIKDDVQNLFLENDDKLLQYTF
                     TNLKSNIAKDSVLQSSLATALQQTNFDYNQIKEQATPNKRLIPALHWYGTQNQFHQWI
                     TNFFKGDFGISYQDGRPVKTVIWKAVRWTLILSIISIILTYLIAVPLGIFSAAKKDSL
                     MDQGISTTLFILYSLPNFWVATLLILFFGGGDFFDIYPANGVGQLEPTMSWIQQFWTR
                     THFLILPVICFTYGGLAFLSRQMRGAMINTLSQDYIRTARAKGLNEEAVLWKHSLKNS
                     LIPIITLFANVFPLLISGAVIIEFKFTIPGMGKTAYEALLARDYPVVYTIVMFSAILT
                     LIGYLIADILYAWVDPRIKFDKK"
     gene            complement(4387..6159)
                     /locus_tag="PLS16_09625"
     CDS             complement(4387..6159)
                     /locus_tag="PLS16_09625"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNJ61382.1"
                     /translation="MSIKKILYIVLFLFLAWFIFGTFHFYGGSKNKVYKDQVVIHNLS
                     DPKGINPLTVSDATTNNYIAPYIFQTLINFDYKTLELVPVLATEVPKVLEKGNLLEVT
                     FNIRPEATWNNGSPITAKDVAFSIKAVMAPKANTVHIQSACDFIKSIITYPEDSRKIT
                     FICDRYMELLTAFPYEIEILPEYIFDPKNILRKYSFEQLKNATEQTKNAKDIDEFVKL
                     FNSDKAARDETMMQGSGAYKLAAFQTGQRVILERKKDWWGDKMNKINHHFEMYPKRLI
                     YEVINDFNTAFTALKNEQLDFMYSTPIKPYIDLDNSSKFTKNFIKSNPVMFGYQCIGM
                     NHKDPILKDQNVRLALAYLTNIDQMIEKVFYNMAERTVASIQPHTEYYNDTIKPYPFD
                     PKKAGQLLKDAGWADTDDDGILDKVIDGKKIKFELKYCYNSGNPIREMIGLLIQKSYK
                     QAGVNIIVQPLDWSLYLNELQKHNVQLWYQGWVAAPGLSDDKQIYHTSSAIDGSNYMS
                     YGNATTDKLLDEIRLEMDKNKRIELVKKWQLIQHEELPYIYLYVQKYRNIIHKRFKGV
                     IESSVYPGIWFGTLQVKDGYKVEK"
     gene            complement(6293..>7665)
                     /locus_tag="PLS16_09630"
     CDS             complement(6293..>7665)
                     /locus_tag="PLS16_09630"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNJ61383.1"
                     /translation="LAKLLLQNPDLLMLDEPTNHLDIESILWLEEFFQKYEGAIILVS
                     HDKTFLDNVCNKTVELELGKMYEYKANYTKYLVLKEERREKQLQTFKNQQDQIKQIER
                     NIERFKAKASKASMAQSLVKKLDRMDVVEVEQDDVRSMRFQFPMGQQSGKIVVLAKKL
                     AKSYGEKDVFHDVNLEIERGDKVAFIGKNGMGKTTMAKIIAKEIEYNSGELTIGHNVN
                     ISFFAQHHAEQLPKALTILQYMEDTAPNEIRAQVRNILGAFMFTGDDVTKKIGVLSGG
                     ERARVCLANLLLNPSNVLILDEPTNHLDLKAKDVLKEAISKYPGTVIVVSHDRDFLVG
                     LTNKVFEFNDGIVKEFVGDVNDFFEERRLASFREVELNPIPKAEPKKIEVVKVDDKEA
                     KRIKNQISKIEESIALKELDIKNIETQIELLSDKNQYDENLIKSLSNAQKELEKLMSD
                     WESLQS"
BASE COUNT         2556 a         1233 c         1083 g         2793 t
ORIGIN      
        1 ggtaatcctt tcaaaacggc ttctctcaaa tctacatcag gggaaacgtg tttggtggaa
       61 tataatttca tactatcgtt ttttacttcg tttaaatttt aagatacgaa gatgctaaaa
      121 taaagataga aattttattt taggatatta agagatattt aggtttttaa tgacggaagt
      181 cggttttttg actaatatgc aatttgtaaa aattggatat ttcgtttgtt ttggcttgga
      241 atcattaaag tattggcatc taattgaact gcttttttag gaataaggtt gagtttttcc
      301 ttttctgtat tgaagatgct gacatgtttt gaattaccca aaggatttat attgtactcc
      361 acaaaattgc ccgtcgcaaa gatatcttcg ttatagataa atttcagtac gttttcagct
      421 tcaaataatg taaaagaaga aaatatggca tcatcatctt cactttgttg ggatttgtaa
      481 atattctttt tccaaagtaa acttccatca ctattaatag aaaaaatcat aatatcaccg
      541 atggtattgt attgagtaga gcgaacttgt tgaggaatat tgctaaaaat tggattatag
      601 aaattagaat aattattgga acgttcttgt cttaaatagg aaaattcgcc ttctacaata
      661 aataacaaac caccatcact tcgtggaata atttttttag gcattaaagt atagatttta
      721 tctctccagt ttttaaaatc ataagtttta ctttctttta catcatcttc agtaagatta
      781 aaatattttt gaactaaaac ttgattgctt tttaaatcta aaatatgata ataaaaaccg
      841 agattatctt tgtttgtagt atttctaaag ccacttaaaa tatataattt atcatcatct
      901 aaatctgttt cggtaattag ttttttatat accgtattgg tgttttttat ttcaacttca
      961 taaattgttt tattcgttgg attgtaataa ttgactagat agctgctgaa atcgtaatca
     1021 tcattactcc atttatcttg atagccaaat acggcactaa caaatccatg attattaatt
     1081 ttcatagaac gtactgaaac attatcttta tctgttacgg taaaaatact tcgtgagatt
     1141 aaagcaatgt cttggttgaa tgttacaaat tgaatatgaa aatttccttt gtttcttacc
     1201 aatgtaaaag tcaataattt tgttctgtcg gcagaagttt ttgcataaaa agtattttta
     1261 ttatcaagat ggatagatag tgaatccaaa attagcgtat tgacttcgtt taaatgctca
     1321 tttaaaactt tagcttttag atattgtaaa tttgaagaat tttgaacata caatacgaca
     1381 attttatcat ctaaaacttg aacagattga atacttttac tctgaacact aatgtctttt
     1441 cgggttttag tattcatttt atcatctaaa atggctaatt gattgttatt gtctccataa
     1501 taatatataa ttgttccaaa attatttttt cctaaaatat cgacatctaa gattttgcct
     1561 ggcaatttct cttcagttga taagctaatt ttttgagcaa aaaccgttga tgaaatactc
     1621 aacattaata tcattatgta aatcgaaaaa aaacgattta atttgaatat tgatattata
     1681 aatttatgag ccatacaatt tacatctaaa ttaaactatt ttttttgtct tgggtctaat
     1741 gcatcagtta aaccttcgcc aattaggtta aataaggtta cggtcaagaa aatcgcaaac
     1801 ccaggaaata ttgcgaccca taattctgat tttccaccac ctgctaatga taacaatttc
     1861 ccccaagtta aagtttcagg cggaatgcct aagcctaaaa atgaaagaaa agattcgatt
     1921 aaaattgcgg cagcaatacc aaaagctatc gttattaaaa ctggtccaac tgaatttgga
     1981 attgcatgac gtaataaaat tcgccatttt ggatagccca atgcttcggc cgcttcaata
     2041 aattctaagc ttctaacctt taataattca gcacgaataa atcttgcaat ttcagtccaa
     2101 gaagttaaac caattacgac catcaccaag aaaatacttg gtttggcaat tgctataatg
     2161 gcaataatta agaataaacc aggaatggaa accaaaactt ctattaatct ttggattaaa
     2221 atatcaacag gaatcgcaat ttttttcttg aaaaatggaa tcggttttag tataaatcct
     2281 aaaaaatagg ccaaaataaa tgaaactaaa attataatta ttcctaataa tattttgcca
     2341 aaagtaaaag cataaaagaa accaaaaaag ccagtaataa taaacataaa tagcgtggca
     2401 cgagatgctt ttaatctgtt atctccaaaa aaaccagcaa tagagcctaa taaaattcct
     2461 attacacttg caattgacat agaaactaag ccaaccataa atgcaattcg tgtgccatga
     2521 attaaagcag aaagtacatc tcgtccaata ctttcagttc ctaaccaatg tctccatctt
     2581 atacttttta catcttgttt gccaaatgga gaaacaaaac caaagtttaa aatatcttga
     2641 gtatttggag aataaggaat tggcggtcgt attgcaaaat caaaatcttc atcaatccaa
     2701 gaaatattat tcagttcagc ataaggcttt ccaattttta aatcgactaa atattgattg
     2761 aaaataggaa aataagtttt gccttcatat ttacaaacaa ttggtttttc gttagccaaa
     2821 acatcagcaa aaatggctat cagtgccata accccgacaa tataaagtga aaataatgct
     2881 cttttattct ttttaaattg acgttttaca tacgaccaat aatcttgttt aagttctaca
     2941 gaatttgctg ttgtcatggc ttatttttta tcaaacttaa ttcgtggatc tacccaagca
     3001 tataaaatat ctgcaattaa ataaccaatt aaagtcaata ttgctgaaaa cataacgatg
     3061 gtataaacaa caggataatc tcttgctaat aatgcctcat acgcagtttt tcccatgccc
     3121 ggaatggtaa atttaaactc tataattacg gcaccactga ttaataatgg gaacacgttt
     3181 gcaaataaag taatgattgg aattaaagaa tttttcaagc tgtgtttcca taaaacggct
     3241 tcttcattca agccttttgc tctggcagta cgaatataat cttgagataa agtattaatc
     3301 atcgcaccac gcatttgtct agataaaaat gctaaaccac cataggtaaa acagattaca
     3361 ggcaaaataa ggaaatgcgt tcttgtccaa aattgttgta tccaagacat tgttggttcc
     3421 aattgaccaa caccatttgc aggataaata tcaaagaaat cgccgccacc aaagaataaa
     3481 attaataagg ttgctaccca aaaatttggc aaagaataga gaataaataa agtagttgaa
     3541 atgccttgat ccattaagct atcttttttt gctgccgaaa agattcctaa aggaacagct
     3601 attaaataag ttaagataat agagattata ctcaagatta aagtccatcg tacagctttc
     3661 caaattacag tttttacagg tctgccatct tgataagaaa taccaaagtc gcctttaaaa
     3721 aaattagtaa tccattggtg aaattgattt tgtgtaccat accaatgtaa tgcaggaatt
     3781 aaccttttgt tcggtgtcgc ttgctcttta atttgattgt aatcaaaatt agtttgttgt
     3841 aaggcggttg ctaaactact ttgcaaaacg ctatcttttg caatattgct ttttaaatta
     3901 gtaaatgtat attgtaataa tttatcatca ttttctaaaa atagattttg tacatcatct
     3961 ttgatattga ttaaaatatc cgcatttgtt gaatcactag gaatatcaga aactaaataa
     4021 gaaaactttt gaacttgatt gtaatatata tttatttcat tccaattgcc atattttttg
     4081 ataaattgat ttaagttttg tcgttgaaat ttttttggta tttcatataa attctttggc
     4141 attgcctgat tggaaattga aaagtagaag acaggtaaat ctaatcccaa ttcgtgtctt
     4201 ttgtcaatat aagcttgttc agaggctttt tgaccagcac tacttccagc atcgttgtta
     4261 gaattgagca tttgttcaac aggatcacca ggtacgttca cactcaaata gaaagtagct
     4321 aatgctatca caaacaaagt tgggataaaa atcaataatc tttttagaat gtattttagc
     4381 atctttttat ttttctactt tataaccgtc ttttacttgt aatgttccaa accaaattcc
     4441 aggataaaca gaactttcga ttacgccttt aaatctctta tgaataatat ttctatattt
     4501 ttgaacatac aaataaatat agggcaattc ttcgtgctga atcaattgcc atttttttac
     4561 taattcaatt cttttatttt tatccatttc taaacgaatt tcatctaata atttatccgt
     4621 tgtagcattt ccataactca tataatttga gccatcgatt gcactagaag tatgataaat
     4681 ttgcttgtca tcgcttaggc caggagcagc aacccaacct tgataccata attgcacgtt
     4741 gtgtttttgc aattcattca agtagagcga ccaatctaat ggttgaacga tgatatttac
     4801 acctgcttgt ttgtagcttt tttgaattaa caaacctatc atctcacgga ttggattacc
     4861 actattatag caatatttta attcaaattt tatttttttt ccatcaatta ctttatcgag
     4921 tatgccgtca tcatcggtat ctgcccaacc tgcatctttt aataattgac ctgctttttt
     4981 gggatcaaaa ggataaggtt taatggtgtc attataatat tcagtatgcg gttggataga
     5041 tgcaacggtt ctttctgcca tattataaaa gactttttct atcatttggt cgatattggt
     5101 taaataggct aaagccaatc gcacattttg gtcttttaat attgggtctt tgtggttcat
     5161 accaatacat tgatatccaa acataactgg atttgatttg ataaaatttt tggtaaattt
     5221 tgatgaatta tctaaatcaa tataaggctt gataggtgtg gagtacataa aatctaattg
     5281 ttcatttttc aatgcagtaa atgcagtatt aaaatcatta attacttcat aaattaatcg
     5341 ctttggatac atttcaaagt gatggtttat tttattcatt ttatcgcccc accaatcttt
     5401 tttgcgttct aaaattactc gttgtccagt ttgaaatgca gccaatttat acgcaccaga
     5461 gccttgcatc atcgtttcat ctctagctgc tttatcagaa ttaaatagtt ttacaaattc
     5521 atcaatatct tttgcatttt tagtttgctc agtagcattt tttaattgct caaatgaata
     5581 ttttctaaga atattttttg ggtcaaaaat atattctgga agaatttcta tttcataagg
     5641 aaatgcagtt aagagttcca tgtacctatc acagataaaa gttatttttc ttgaatcttc
     5701 agggtaagta ataattgatt tgataaaatc gcaagcagat tgaatgtgaa cagtattggc
     5761 tttaggcgcc atgactgctt taatagaaaa tgccacatct tttgcagtaa ttggtgagcc
     5821 attgttccaa gttgcctctg ggcgtatatt aaaagtaact tctaggagat tgcctttttc
     5881 taatactttt ggtacttctg ttgccaaaac tggcaccaat tctaaagttt tataatcaaa
     5941 attaattaat gtttgaaaaa tataaggagc aatgtaatta ttggttgttg catcagaaac
     6001 ggttagagga ttgataccct ttgggtccga aagattatga ataacaactt ggtctttata
     6061 tactttattt ttgctaccgc cataaaaatg aaatgttcca aaaataaacc aagccaaaaa
     6121 taaaaataga actatatata aaattttctt tatgctcatt aaatgttagt cttccttaca
     6181 aatgtaataa gtttatggaa tttaatttaa tttatattat ctaaaagtaa aaaacgaaaa
     6241 ataaattact taaaatcaaa aaaacaaata gaatctttag ggtaagtttt gactaacttt
     6301 gtaaactttc ccaatcgctc attagttttt ctaattcctt ttgtgcattg ctcaagcttt
     6361 taattaaatt ttcgtcgtat tggtttttat cggacaataa ttcaatttga gtttcaatgt
     6421 ttttaatatc caattctttt aaagcaatag attcttcaat ttttgaaatt tgatttttaa
     6481 ttctttttgc ttctttatca tctactttga caacttcaat ttttttgggt tctgctttag
     6541 gaataggatt tagttccact tctctgaatg atgctaatct tctttcctcg aagaaatcgt
     6601 ttacatcacc gacaaattcc tttacaatac catcattaaa ttcaaatact ttatttgtca
     6661 agcccaccaa gaaatctcta tcgtgcgaaa ccacaataac ggttcctgga tatttagaaa
     6721 ttgcttcttt taatacatct ttagctttca aatctaagtg attggttggc tcatcgagta
     6781 ttaaaacatt acttggattt aataataaat ttgccaaaca aactctagca cgttcaccac
     6841 cactgagtac cccaattttt ttagtaacat catctccagt aaacataaat gcacctaaaa
     6901 tatttcgcac ttgagcccga atttcatttg gtgctgtatc ttccatatat tgtaaaatgg
     6961 tcaatgcttt tggcaattgt tctgcatggt gttgagcaaa aaatgatata ttgacgttgt
     7021 gtccaatggt caattcgcca ctattgtatt caatttcctt ggcaataatt ttagccattg
     7081 ttgtttttcc cattccattt ttccctataa atgcaacttt atcaccacgc tcaatttcta
     7141 aatttacatc atggaaaaca tctttttctc cgtagctttt cgcaagtttt tttgctaaga
     7201 caacaatttt accactttgt tgtcccatcg gaaattgaaa acgcatagaa cgcacatcat
     7261 cttgttctac ttcaaccaca tccattctgt cgagtttttt taccaaactt tgagccatac
     7321 tagctttact tgctttggct ttaaatcgtt caatatttcg ctcaatctgt ttaatttggt
     7381 cttgttggtt tttaaaagtt tgcaattgct tttctcttct ctcttctttt aaaactaagt
     7441 atttagtgta attggctttg tattcataca ttttgcccaa ttctaattct actgttttat
     7501 tacatacatt atctaaaaaa gttttatcgt gtgaaactag gataattgct ccttcatatt
     7561 tttgaaaaaa ctcttctaac caaagtatcg attctatatc taaatggttg gttggctcat
     7621 cgagcattag taaatcagga ttttgtaaca ataactttgc taatt
//
LOCUS       DAOQCW010000117         7641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_3808_length_7641_cov_4.792908, whole genome shotgun sequence.
ACCESSION   DAOQCW010000117 DAOQCW010000000
VERSION     DAOQCW010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7641
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3808_length_7641_cov_4.792908"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1597
                     /locus_tag="PLS16_09635"
     CDS             <1..1597
                     /locus_tag="PLS16_09635"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNJ61384.1"
                     /translation="IKTKINTNSTAYKDNYQKMMDKIGTLQKHLQNSLFQGEDKHKER
                     AKSKGKLLARERIEMILDIDSPFLELCPLAGLEVKGGFGTGGTMVAGIGFVSGKMCMI
                     TANVGTHKGGAIDISSLQKAIRIGEIAKENNLIGINLVESAGANLPDQAKVFNLGGNN
                     FKEITRRSKAGIPTISIVFGNSTAGGAYIPGMSDYTIFIKNQAKVFLAGPPLVKMATN
                     EIVDDETLGGAEMHSKISGVSDYLANDELDGIRIARELVDNLDTPQNTDNSINKPYKE
                     PLYPIDELLGIISVDPKIPFDCREVIARLVDGSEFHEFKKDYGTTLICGYAKIHGYPI
                     AIISNNGVLFNDSSNKGAHFIQLCNMNNTPIVFLQNITGFMVGKQYEQAGIIKDGAKM
                     INAVSNSEVPMFTIMMAASYGAGNYAMCGRAYNPRFLFSYPNSMIAVMGSEQLAGVMQ
                     MVAKESAIKSEKQYDEQQAEQMKAFMQAEIEKQSNAFFASGQLWDDGIIDPRDTRHIL
                     GLVLALTYMNEVKGTNAWGVYRM"
     gene            complement(1675..2805)
                     /locus_tag="PLS16_09640"
     CDS             complement(1675..2805)
                     /locus_tag="PLS16_09640"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNJ61385.1"
                     /translation="MKIAIIGGGIGGLTTALALKQNGQNITIYESASEIKPVGAGIIM
                     ANNAMQVFDKLGVREKIENAGCKISDIKITDAQLNTISESNLTKFEEKFGVHNVAIHR
                     ADLQKILADEIGLENIQLSKRLLKIEQENDFKLTFDDNTSIHADIVIGADGIKSTVRT
                     QLFGTRNLRDTKQRCWRGVSELDSTSKYNQTAIEAWGKGKRFGFVKISSKKVYWYAVI
                     NEELKNKHSNIAEFFNDFHPEILKMITETPKDNIIFSDIIDMQPSKQWQKGKVCLIGD
                     AAHATTPNMGQGACQAVEDAYILGKLFGQRKCVEDVFTQYEKLRMEKAHFIVNTSWKI
                     GKIAHFQNSMAVWLRNALVKAMPKSSSEKQLEKVFSIEYDIS"
     gene            complement(2991..4271)
                     /locus_tag="PLS16_09645"
     CDS             complement(2991..4271)
                     /locus_tag="PLS16_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNJ61386.1"
                     /translation="MELVIILLLIILNGIFAMSEMALVSSKKFKLESAKKKGSSGAKK
                     ALELAETPTRFLSTVQIGITLIGILLGVFSGKNLSDNVVQLINKVDFLAPYAQSIATV
                     VVVLLITYLSIVLGELLPKRLGMTFPEPIISLFAKPMYYLSILTSPFVWILTMSNNML
                     LSVLGINSMSNSTVSEEEIKAMVKESAEGGEIEGIEHEIVERVFELGDRKVNSLYTHR
                     TDLVYFNTSDTWETVVTTINEEKHSVYPVVENHKLDEIIGIVKLKDLFNVYSDSSNFD
                     INNYLIKPIYVTENTSAYKILEMFKNEKMHYAIVVNEYGTTVGIVTMDDVLDALVGDS
                     TEHGQEEYQIVQRDENSWLVDGQYPLVEFVKTFELDIDDFLLEKYSTIAGLVIHHLSS
                     VPNVGERCTIEGYIFEIVDKDGQRIDKLMVSVAE"
     gene            4549..5652
                     /locus_tag="PLS16_09650"
     CDS             4549..5652
                     /locus_tag="PLS16_09650"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HNJ61387.1"
                     /translation="MIVSIPKETKFKENRVAITPLIAKDLIAKGFKVNVETDAGLKSF
                     FSNEAYKEVGVNLVDKQEAYSSADIVLKVNAPQADEIALMKKGAVLISFMFAGTNPEL
                     VDACSKAGISAFSMDAVPRISRAQKMDALSSQANLAGYKSVLMCANEIGKILPMMTTA
                     AGTIKPSKVVIFGAGVAGLQAIATAKRLGAVVEVSDIRPETKEQVESLGGKFIEVKGD
                     DSVKMEGGYVKGVSEDFLKKQQELVGKHVAEADIVITTALIPGRKAPVLVTEEMLSKM
                     KFGSVVLDMAVEQGGNVIGSKLNENVVINGVTIIGEGNIPSLLPMNASDLYAKNIQTL
                     LYHLATSEGFKWEMEEEITKGSLIVHNGEKRIG"
     gene            5682..5987
                     /locus_tag="PLS16_09655"
     CDS             5682..5987
                     /locus_tag="PLS16_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNJ61388.1"
                     /translation="MEEILNLFVTHREMIYFIILAAFVGVEVIGGVPTVLHTPLMSGA
                     NAIHGVVIVGAIIVMLDTDPNNILGLGLGFLAVILGTLNVVGGFVVTDRMLEMFKKK"
     gene            6069..7460
                     /locus_tag="PLS16_09660"
     CDS             6069..7460
                     /locus_tag="PLS16_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HNJ61389.1"
                     /translation="MQGLLEIAYLIGSITFVIGLKMMGNAKTARQGNLIGAFGMTVAI
                     LGTIFLFKTEHTSREAIKLALIFGGIAIGSVIGWITAKKVEMTKMPELVSMFNGMGGA
                     CAALIGLTEYEHNLGKIGSLSFIIAGLVIGTISFTGSIIAYLKLAGKMKKPIRIPMYN
                     ILNNVILLGVVAFSAFVVIQNQSLPLTIVWVLFAVAAVYGILFTIPIGGADMPVVISL
                     LNSFTGLAAAFGGFLYDNKPMLTGGILVGSAGTLLTLAMCKAMNRPLSNVIFGAFGGG
                     SGAAADGGEKGTVKDVQVSDLAVQLNYASKVVIVPGYGLAVAQAQHVIHELEKLLEER
                     GVEVKYAIHPVAGRMPGHMNVLLAESNVDYDKLVEMEDINPQFVQTDVVLVVGANDVV
                     NPAAKVDPSSPIYGMPILEVENAKNIVINKRSMNAGYAGIDNLLFYNPKTSMFFGDAK
                     KALTELVAEIKQL"
BASE COUNT         2626 a         1178 c         1275 g         2562 t
ORIGIN      
        1 tattaaaaca aaaatcaata ccaattccac cgcatacaaa gacaactatc aaaagatgat
       61 ggacaaaatt ggaacattac aaaaacatct acaaaattct ttatttcaag gagaagacaa
      121 acataaagaa cgtgcaaaat caaaaggaaa attactagct cgtgaacgta ttgaaatgat
      181 attagatata gattctccat ttttagaact atgtcctttg gcaggtttgg aagtaaaagg
      241 aggattcggc actggaggta ctatggtagc aggaattgga tttgtttctg ggaaaatgtg
      301 tatgattact gcaaatgttg gcacgcataa aggaggagcc atagatattt caagtttgca
      361 aaaagcgatt agaattggag aaattgcaaa agagaataat ttaataggta taaatttagt
      421 ggaaagtgca ggtgccaatc tcccagacca agcaaaagta tttaatttag gtggcaataa
      481 ttttaaagaa attacacgcc gttcaaaagc tggaatacca acaatttcaa ttgttttcgg
      541 taattcaacc gcaggtggtg cctatatccc aggaatgtca gactatacta tttttataaa
      601 aaatcaagca aaagtatttt tagcaggtcc acctttagta aaaatggcta ccaatgaaat
      661 agttgatgac gaaacattag gtggtgcaga aatgcatagt aaaatttcgg gtgtttcaga
      721 ttatttagcc aatgatgaac tagacggcat tagaattgca agagaattag tagataactt
      781 agatacacca caaaatacag acaactcaat caacaagcca tacaaagaac cgctttatcc
      841 tattgatgaa ttattaggca taatttctgt cgatccaaag ataccttttg attgtagaga
      901 agtcattgcc cgtttggtag atggttcaga atttcatgaa tttaaaaagg attatggtac
      961 aacgctcatt tgtggttatg ctaaaattca tggctatcct attgctatta tttccaacaa
     1021 tggtgtactt tttaatgatt catcaaataa aggtgctcat tttattcaac tgtgtaatat
     1081 gaacaatacg ccaatagtat ttttacaaaa tatcacagga tttatggtgg gtaaacaata
     1141 tgaacaagct ggaataatta aagatggtgc aaaaatgatt aatgcagttt ccaattcaga
     1201 agttcctatg tttacaatta tgatggcagc aagttatggt gctggaaatt atgccatgtg
     1261 cggacgtgcg tacaatcctc gatttttatt ttcttatcca aactcgatga ttgctgtgat
     1321 gggtagcgaa caattagctg gtgtaatgca aatggtagca aaagaaagtg ctataaaatc
     1381 agagaaacaa tatgatgaac aacaagctga acaaatgaaa gcatttatgc aagctgaaat
     1441 tgagaaacaa agcaatgctt tttttgctag tggacagctt tgggatgatg gcatcattga
     1501 cccacgagat acacgacata ttcttggttt ggtattagca ttaacttata tgaacgaagt
     1561 taaaggtaca aatgcttggg gtgtgtatcg tatgtagtgg atagtttcgg tctgaaactg
     1621 attcaaacat taataagtcg atactacaaa tattgtagaa atttgggctt atcgtcaaga
     1681 aatatcgtat tcaatgctaa aaactttctc taattgcttt tcgctagaag atttaggcat
     1741 tgcttttact aaagcatttc tcaaccaaac tgccatacta ttttgaaaat gtgcaatttt
     1801 tccaattttc caactggtat taacaataaa gtgggctttt tccattctta gtttttcata
     1861 ttgagtaaaa acatcttcca cgcatttcct ttgtccgaat aattttccta aaatataagc
     1921 atcttcaaca gcttgacaag caccttgtcc catattcggc gtagtagcat gtgcagcatc
     1981 gcctattaaa caaactttgc ctttttgcca ttgtttgctt ggttgcatgt ctataatatc
     2041 gctgaatatg atattgtctt ttggtgtttc agtaatcatc tttaaaattt ctgggtgaaa
     2101 gtcattaaaa aattcagcaa tatttgaatg cttatttttt aattcttcat tgattactgc
     2161 ataccaataa acttttttag aactgatttt tacaaaacca aaacgttttc ctttacccca
     2221 agcttcaatt gcagtctggt tgtatttaga agtagaatcc aattcactta cgccacgcca
     2281 acatctttgt tttgtatcac gaagatttct cgtaccaaac aactgagttc taacggttga
     2341 tttaattccg tccgctccaa ttacaatatc agcatggata ctcgtattgt cgtcaaaagt
     2401 tagtttaaaa tcattttctt gttcaatttt caacagtcgt tttgataatt gtatattttc
     2461 gagacctatt tcatcagcta aaattttttg taaatctgca cggtgaatgg ctacgttgtg
     2521 aacaccaaat ttttcttcaa attttgtaag attgctttca gaaattgtat ttaattgagc
     2581 gtctgtaatt ttaatatccg aaattttaca accagcattt tcaatttttt cacgaacgcc
     2641 aagtttgtca aaaacctgca ttgcattgtt tgccataata attcctgcac caacaggttt
     2701 aatttcagaa gcactttcat aaatagttat attttgtcca ttttgtttta aagccaaagc
     2761 cgtagttaaa ccaccaatac caccacctat gattgctatt ttcattattt ttctttttta
     2821 aatctacaac aaaaatatat cactataagt tacaaatcat tcactttaga taaataattt
     2881 gttattatat gctgtttata aggtccaaaa atcattttac tagtttttat ttttacacac
     2941 attcttttgg ttattaatgt gtaaatctag tttaagaaaa gaatacaagt ttattctgca
     3001 accgaaacca ttaatttatc aattcgttgt ccgtctttat ctacgatttc aaagatataa
     3061 ccttctatcg tacaacgctc accaacattt ggaacagaag acagatgatg aatgactaat
     3121 ccagcaattg ttgaatattt ctctaaaaga aaatcatcta tatccaattc aaaagttttt
     3181 acaaattcca ctaatggata ttgtccatct accaaccaac tattttcatc tctttgtaca
     3241 atttgatatt cttcttggcc atgttcagta gaatcgccaa ccaatgcatc taaaacatca
     3301 tccatagtaa caatacctac agttgttccg tattcattta cgactatggc ataatgcatt
     3361 ttttcattct taaacatctc caaaatttta taggcagatg tattttcagt tacatatatt
     3421 ggcttaatca gataattatt tatatcaaaa ttactactat cagaatatac attaaacaaa
     3481 tctttaagtt taacgattcc aataatttca tctaatttat gattttcaac aacaggataa
     3541 accgaatgtt tttcttcatt tattgttgta actactgttt cccaagtatc tgaagtattg
     3601 aaataaacca aatccgttcg atgtgtatat aaagaattta cttttctgtc tcctaattca
     3661 aaaacacgct caacaatttc atgttctata ccttcaattt cgccaccttc agcactttct
     3721 tttaccatgg ctttaatttc ttcttcactc actgtactat ttgacatgga attaatccct
     3781 aaaacgctca ataacatatt atttgacatc gttaaaatcc atacaaatgg agatgtcaaa
     3841 atagataaat aatacattgg cttagcaaat aaactaataa ttggttcagg aaatgtcata
     3901 cctaaacgtt taggcaacaa ctctcctaaa acgattgaca aatatgtaat taataaaaca
     3961 actacaactg ttgcaatact ttgtgcgtaa ggtgctaaaa aatcaacttt attaataagt
     4021 tgaacaacat tatctgatag atttttacca ctaaaaaccc ccaataaaat accaattaag
     4081 gtaattccaa tttgtacagt agataagaat cgtgtaggcg tttctgccaa ttccaatgct
     4141 ttttttgctc cagaacttcc tttcttcttt gcactttcta atttgaattt tttagaagaa
     4201 actaatgcca tttcagacat tgcaaaaatt ccatttagta taatcaataa taaaataatt
     4261 actaattcca tgttttagat ggtttatgat taacacataa aatactattt attcttgatt
     4321 ttgagagaga taggttatta aagcacctcg aggaactgtc catttgctat attaaatttt
     4381 aggctaaatt aaataacaat ttctaaaatt tgctaataaa atttttaata caaaattatt
     4441 ttactaaagg aatcccgttt tttaaaagat tttaacaaat ggtaaataaa ttatagaaat
     4501 tttaaaatct aaatatataa tcttattttc gagaaaattt ttttaaccat gatagtttca
     4561 atacccaaag aaaccaaatt taaagaaaac agagttgcca taacgccttt aattgcaaag
     4621 gatttaattg caaaaggatt taaagtaaat gttgaaacag atgctggctt aaaatctttc
     4681 ttttctaatg aagcttacaa agaagtaggt gtaaatttgg tggataaaca agaagcatat
     4741 tcaagtgcgg atatcgtact taaagtaaat gccccacaag cagatgaaat tgccttaatg
     4801 aaaaagggtg ctgtattaat ttcatttatg tttgctggaa ccaatccaga attagttgat
     4861 gcatgctcaa aagctggtat ttctgcattc tcaatggatg cagttccacg tatttcacgt
     4921 gcacaaaaga tggatgcctt atcctctcaa gctaatttgg caggttataa atctgtatta
     4981 atgtgtgcaa atgagattgg gaaaatctta cccatgatga caacagctgc tggaacaatt
     5041 aaaccttcaa aagtagtcat ttttggtgca ggtgttgcag gattacaagc cattgcaact
     5101 gctaaacgtt taggtgcagt tgtagaagta tcagatattc gccctgaaac caaagaacaa
     5161 gtagaatctt taggtggaaa attcattgag gtaaaaggtg atgattctgt aaaaatggaa
     5221 ggtggctacg taaaaggtgt ttctgaggat ttcttaaaga aacaacaaga actagttgga
     5281 aaacatgttg cagaagctga tatcgttatc acaacagcat taattccagg aagaaaagca
     5341 ccagttttag ttactgaaga aatgctcagt aaaatgaagt ttggttctgt agttttagac
     5401 atggcagtag aacaaggtgg taatgttatt ggtagtaaat taaatgaaaa cgttgtcatt
     5461 aatggtgtta ctattattgg cgaaggaaat attccatcat tattacctat gaatgcgagc
     5521 gacctttatg ctaaaaacat tcaaactcta ttataccatt tagcaactag cgaaggtttt
     5581 aaatgggaaa tggaagaaga aattacaaaa ggtagtttaa ttgttcacaa cggagaaaaa
     5641 cgtattggat aatattatta ataaagaata aaataatcaa aatggaagaa atattaaatc
     5701 tatttgtaac gcacagagaa atgatttact tcataatcct agctgcattt gttggtgtgg
     5761 aagttattgg tggcgttcca actgtattac atactccttt aatgtctgga gctaatgcca
     5821 ttcacggtgt tgttatcgtt ggagcaatta tagtaatgtt agatacagat cctaataata
     5881 tcttaggatt aggattaggc tttttagctg taattcttgg aacattaaac gtagttggtg
     5941 ggtttgtggt aactgacaga atgttggaaa tgtttaagaa aaagtaaatc aaattgaaaa
     6001 tgtaatattg aaactataaa tagttcatta agacattttc aaattttcaa attttcaaat
     6061 taaaaaaaat gcaaggatta ttagaaatag catatttaat tggttcaatt acatttgtga
     6121 ttggattaaa aatgatggga aatgccaaaa cagcaagaca aggtaactta attggtgctt
     6181 ttggtatgac agtagccatt ttgggtacta ttttcctttt taaaacagag catacctcaa
     6241 gagaagctat caaattagct ttaatttttg gaggtatagc tatcggttct gtaataggtt
     6301 ggattacagc caaaaaagta gaaatgacca agatgccaga attggtgtct atgtttaatg
     6361 gtatgggtgg tgcttgtgct gccttaattg gattaactga atacgaacat aatttaggta
     6421 aaatcggttc tttaagtttt atcattgcag gattagtaat tggtacaata tcatttaccg
     6481 gttctatcat tgcttatttg aaattagctg gaaaaatgaa aaagcctatc cgtattccaa
     6541 tgtataatat attaaacaat gttatcttat taggtgtagt tgcattttca gcatttgttg
     6601 ttattcaaaa tcaatctttg ccattaacaa ttgtatgggt attatttgca gttgctgctg
     6661 tatatggtat tttatttacc attccaattg gtggtgctga tatgccagta gttatttcat
     6721 tattaaactc atttacaggt ttagcagccg catttggagg tttcttatac gacaataagc
     6781 caatgctaac aggtggtatt ttagtaggtt ctgcaggtac acttttaact ttagcaatgt
     6841 gtaaagcgat gaatagacca ttaagcaatg taatcttcgg agcattcggt ggtggtagtg
     6901 gtgcagccgc tgatggtgga gaaaaaggaa ccgttaaaga tgttcaagta tctgacttag
     6961 cggttcaatt aaactatgca agtaaagttg ttattgttcc aggttatggt ttagcggtgg
     7021 ctcaagcaca acatgttatc catgaattag aaaaattatt agaagaaaga ggtgttgagg
     7081 taaaatatgc tatccaccca gttgctggtc gtatgccagg acacatgaac gttttattag
     7141 cagaatctaa tgtagattac gataaattgg tagaaatgga agacatcaac ccacaatttg
     7201 tacaaacaga tgtggttttg gttgttggag ctaatgacgt tgtaaaccca gctgctaaag
     7261 tagatcctag ttctccaatt tatggaatgc caattttgga agtagaaaat gctaaaaata
     7321 ttgttattaa taaacgttca atgaatgctg gatatgcagg tattgataat ttattattct
     7381 acaatccaaa aacatctatg ttctttggag atgcgaaaaa agcattaaca gaattagtcg
     7441 cagaaatcaa acaactgtaa tatttagcta tgttatataa ataaaaatgg ctcacgaaag
     7501 tgagtcattt tttatttaaa attcgaaaga ttataagaat ctgttcttat cattttttat
     7561 atctcgcaat acgctataca ccatgttttc tgcacaaaac tcacctaaag tagcgtattt
     7621 attgacataa ttttctttca a
//
LOCUS       DAOQCW010000118         7580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_3849_length_7580_cov_6.023123, whole genome shotgun sequence.
ACCESSION   DAOQCW010000118 DAOQCW010000000
VERSION     DAOQCW010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7580
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3849_length_7580_cov_6.023123"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..397)
                     /locus_tag="PLS16_09665"
     CDS             complement(26..397)
                     /locus_tag="PLS16_09665"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0090305 - nucleic acid phosphodiester bond
                     hydrolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HNJ61390.1"
                     /translation="MAEKKKVTANEIYDLLLNEFKIKEQIGSIEIILGGISAKYKGKD
                     AIGDLLQEWLGEWLRQQNFYFRQPTNTQEFPDFLLTESSNSGFLELKTFNADASPAFD
                     IANFDSYCTSLLTIDLLRNSF"
     gene            complement(630..2060)
                     /locus_tag="PLS16_09670"
     CDS             complement(630..2060)
                     /locus_tag="PLS16_09670"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF006139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNJ61391.1"
                     /translation="MTTNWVNKEKNAIELINIAGKLWLEKNIELTIFRRNIADARFTE
                     ILNNAEFTRVYLNLPITVDDFLAIAQSIEKLNLAPTRVDLSTLGKEWLEEKANFDSID
                     IFVAKKLEAYIGQDKKSLKPKHVVLFGFGRIGRLVARELIQQYGKGDQLVLKAIVTRG
                     NSDDEIKKRSSLLRKDSVHGKFRGVITEDYANKALIANGNTIHMIAANNPAEIDYTQY
                     GIQDALLIDNSGIFRDEEKLASHLQAKGISKVLLTAPGKGSLPNIVYGVNHLAFNRDE
                     KNIFTAASCTTNAISPVIKVIKDHYGIESGHVETVHSYTNDQNLLDNYHKKYRRGRSA
                     PMNMVITETGAASAITKVIPEMAGKFTGNAVRVPTPDVSLAILNLNLSKEASSVEEVN
                     QLLRNAGSFGDLVEQIDFSTSQELVSSDCIGNSHASIVDSNATLISPNNKSIVLYVWY
                     DNEYGYTRQVVRLAKYIGGVQRLTYY"
     gene            complement(2104..2973)
                     /locus_tag="PLS16_09675"
     CDS             complement(2104..2973)
                     /locus_tag="PLS16_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HNJ61392.1"
                     /translation="MRQLKITKSITNRESQSLEKYLQEIGKVELLTPEEEVELAQRIK
                     AGDQIALEKLTKANLRFVVSVAKQYQNQGLTLSDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNKVGSLNKINKAFSNLEQEFEREPSAEELAE
                     MLEITREEVETTLGVAARHVSVDAPFQEGEENSLLDVLENTSVPSTDYSVEYLDSLRK
                     EIDRSLSTLTDRQKDVLIYYFGIGMEHPLSLEDIGEKFALTRERVRQIKDKAINKLRA
                     TSKSKLLKNYLGN"
     gene            3093..4529
                     /locus_tag="PLS16_09680"
     CDS             3093..4529
                     /locus_tag="PLS16_09680"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNJ61393.1"
                     /translation="MLKKLDKVVIRSYILPLVITYLLTTFIFVMQFLWKYIDDIVGKG
                     LELSLIAKLVVLFAIIILPMALPLAVLLASTMVIGNLAESNELTAYKASGISLIRVMF
                     GLIFSALLMAIFAFYFSNRISPYVNLKFYTLLYDIRKQKPALDITEGVFYRGISNFAI
                     KVKKKDKDNKTLYDIIVYDHTNSDNNSIVTAKKGEVLLSNTGQYLILNLYEGSQYQFK
                     PNINKQDGSVKEMTQLSFKKWRKIFDLKEFKMERTDESLFKSNYAMYNSAQLRLLSDS
                     VQMELNKSESNINQTIKNNFVPFKQDDISLSENDIMLKNISINLDSIYARNIDVRGTT
                     MALGLVNGVIANIENSRDDIKWKLSDMAQYQIELYRKFSLSFACIVLMLIGAVMGAIV
                     RKGGFGVPVLIAVLYFVAFHFFNVIGEKLAKNEVLPVAIGMWLSAIVLFPIALFLTYK
                     AMNDSVIFRIESIQKTFHILKKKLPKAK"
     gene            4526..5668
                     /locus_tag="PLS16_09685"
     CDS             4526..5668
                     /locus_tag="PLS16_09685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ61394.1"
                     /translation="MIIFNILLLFSLFALVYSYFLYPLLLKIISSFNLGKQSISIDNH
                     IDIKYKDINIIIPVHNEESIIGSKLKSIVNQNYPKEKIKIYIGLDACTDNSIKEIEYI
                     DSQLNIIKFESKTRIGKPKLLNHLFELIQDEESIILVTDADILLETNTLIDISKSFDN
                     ELNILTDLKLINKNIEAIEENLYLDLENTIKSCESKNFHIFQGVSGACYAIKRKYFKP
                     IPENFLVDDFYISIQALMQQPFATFLENSFVYENRPKDMSLEFKRKIRIATGNFQNLY
                     FFGTKLLNPISAIGFTFISHKLFRWLTPFFMLYISIYLLINYTFIILALTLILLSIVF
                     FLSIFEVKKFVRIPFYFIAMQIAVLIGFYRFLKGVKTNIWQPTNRI"
     gene            5670..6866
                     /locus_tag="PLS16_09690"
     CDS             5670..6866
                     /locus_tag="PLS16_09690"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNJ61395.1"
                     /translation="MLDTIESALEDIKKGKLIIVVDDEDRENEGDFVTAARNVTPEII
                     NFMATHGRGLICAPIIEDECDRLGFEMMVNSNSSHFDTPFTVSIDLIGNGCTTGISTA
                     DRAKTIQALVDPNSKPSDFGKPGHIFPLKAKKEGVLRRTGHTEATIDLARLAGFEPAG
                     VLVEIMNEDGTMARTPELLEIAKRFDLKIISIKDLVEYRLKHEKQIERVVEVNMPTKY
                     GDFKLTAYKDIQTGEEHFALTKGTWEKDEPVLVRVHSSCITGDILGSLRCDCGDQLHG
                     AMEMVQEAGKGVILYMMQEGRGIGFFNKLRAYKLQEEGMDTVDANLALGFKADQRDFG
                     IGAQILRDLNISKLKLITNNPKKRAALGGYGLEIVENIPIKIAPNKHNKKYLDTKRDK
                     MGHELR"
     gene            6971..7534
                     /locus_tag="PLS16_09695"
     CDS             6971..7534
                     /locus_tag="PLS16_09695"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNJ61396.1"
                     /translation="MSKILMLDNYDSFTYNIVHYVESFDFKVDVFRNDKIGIEAINQY
                     DKIILSPGPGIPEEAGILLDVIKTYAPTKCILGICLGHQAIGQVFGAELYNVGQVIHG
                     KTRNTIIKEQDIIFDGLDTNFESGRYHSWAIKNIQAPLQVTAIDDDGIVQAIRHQEYN
                     VRGVQFHPESVMTPNGKKIIENWLFKC"
BASE COUNT         2592 a         1107 c         1167 g         2714 t
ORIGIN      
        1 tattgcagga atttataatc taagtctaaa atgaatttcg caagaggtct attgttaaaa
       61 gtgaagtgca ataactatca aaatttgcaa tatcaaaagc tggacttgca tctgcattaa
      121 aagtttttaa ttctaaaaaa cctgaatttg aactttctgt taatagaaag tctggaaatt
      181 cttgagtgtt tgttggttgt ctaaaataaa aattttgttg ccttaaccat tctccaagcc
      241 attcttgaag taaatcacca attgcgtctt tgcctttata ttttgcagaa attccgccaa
      301 gtatgatttc aatacttccg atttgttctt ttattttaaa ttcattcaac aacaaatcat
      361 aaatttcatt cgctgtaacc tttttcttct ctgccatcaa tgtaacttga ttcgatttaa
      421 aatttttcgt aaatgtacag tttttttgtt gatgttcatt tgtaaatatt tggttctata
      481 atattgtaat tcaacaatga gataactctg tgttgcaata atttataaat cgaataattg
      541 aattatctat ttctaatctt acaaaaactc ctcaaataaa aatcccgacc aaatagtcgg
      601 gacttgattt attatctttt ctattttaat taatagtaag tcaatctttg aacaccgcca
      661 atatatttgg ctagtcttac tacttgtctt gtgtaaccat attcattatc ataccaaaca
      721 tacaatacaa tagatttgtt atttggcgaa attaaagtcg catttgaatc tacaattgat
      781 gcatgtgaat tgccaataca atcagaggaa actaattctt gagaagtaga aaaatcaatt
      841 tgttctacta aatctccaaa agaacctgca tttcttaata attgattcac ttcttcaaca
      901 gaacttgctt ctttagataa attcaaattt aaaatagcta aagaaacatc aggtgtagga
      961 acacgaaccg catttccagt gaattttcca gccatttcag gaataacttt tgtaatcgca
     1021 cttgctgcac cagtttccgt aattaccata ttcattggag cagaacgacc tcttctatat
     1081 tttttatggt agttgtccaa taaattttgg tcattagtat aagaatgcac tgtttcaacg
     1141 tgtccacttt cgatgccata atgatcttta attactttaa ttactggaga aattgcattt
     1201 gttgtgcaag aagctgccgt aaatatattt ttctcatctc tattgaatgc taaatgatta
     1261 acaccataaa cgatatttgg taaacttcct tttccgggtg ccgtcaataa aactttagaa
     1321 ataccttttg cttgtaagtg tgatgctaat ttttcttcat ctctgaaaat tccagaattg
     1381 tcaatcaata aagcatcttg aattccatat tgcgtataat caatctcggc aggattgtta
     1441 gctgcaatca tgtggatagt attaccattt gcaattagtg ctttgttggc ataatcttca
     1501 gtgataacac ctctaaattt tccatgaaca gaatcttttc taagtaatga agaacgtttt
     1561 ttgatttcat catcagaatt tcctcttgta acgattgctt ttaaaactaa ctgatcccct
     1621 tttccatatt gttgaattaa ttctcttgct actaaacgac caattctacc aaacccaaaa
     1681 agtacaacat gttttggctt caaacttttc ttgtcttgac caatataagc ttctagtttt
     1741 ttagcaacaa atatatcaat agaatcgaag ttggcttttt cttccaacca ttctttacct
     1801 aaagtagata aatcaactct tgttggagca aggtttaatt tttcaatgct ttgtgcaata
     1861 gctaagaaat catcaaccgt aattggtaag tttaaataaa ctcgtgtaaa ttctgcatta
     1921 ttcaaaattt ctgtgaatcg tgcatccgca atgtttcttc tgaaaatcgt taattcaatg
     1981 tttttttcta accaaagttt gcctgcaatg ttaattaatt caatggcatt tttttctttg
     2041 tttacccaat ttgttgtcat gattttaaag ttgtatatta atttttagag gtttaaataa
     2101 aaattagtta ccaagatagt ttttcaataa cttactcttt gaagttgcac gtaatttgtt
     2161 tatcgcttta tctttaattt gtcttactcg ttctcttgtt aatgcaaatt tctctcctat
     2221 atcttctaat gaaagtggat gttccattcc tattccaaaa tagtaaatca aaacatcttt
     2281 ttgtcggtct gtaagtgtac ttaaagaacg gtcaatttct tttctcaaag aatccaaata
     2341 ctcaacagaa taatctgtac ttggaacact agtattttcc aaaacatcta aaagtgaatt
     2401 ttcttcacct tcttggaaag gagcatcaac cgatacgtgt cttgctgcaa cacctaaggt
     2461 cgtttcaact tcttctcttg taatttctaa catttcagcc aattcttcag cagatggctc
     2521 acgctcaaac tcttgttcta aatttgaaaa tgctttattg attttgttta atgagccaac
     2581 tttatttaat ggtaaacgaa caattcttga ttgttccgct aaagcttgta aaatagattg
     2641 acgaatccac caaactgcat aagaaataaa tttaaaacct ctagtttcat caaatcgttg
     2701 agcagcttta atcaaaccca aatttccttc attgattaaa tcgctcaaag tcaaaccttg
     2761 attttgatat tgttttgcaa cagaaaccac aaaccttaaa tttgctttag tcaatttttc
     2821 taaggctatt tggtctccag ctttaattct ttgtgctaac tctacttcct cttcaggtgt
     2881 tagcaactcc actttcccaa tttcttgtaa atacttttct aaagattgac tttctctgtt
     2941 ggtaatggat ttggtaattt ttaattgacg catatcgaaa aactctgttt tttttgtgaa
     3001 gactgcgaat ttaattaaaa tctttctata tttcaagaat ttaacattat taaaacgtaa
     3061 gaaattaagt attttagcac ttcatttttg ttatgctaaa gaaattagac aaagtagtta
     3121 tccgttccta tattttacca ttggtaatca cttatttact gaccactttt atatttgtca
     3181 tgcaattttt atggaagtat attgacgata ttgttggtaa aggattagaa ttgagtttga
     3241 tagcaaaact agtcgtttta tttgcaatta tcattcttcc aatggcattg cctttggctg
     3301 ttttgctggc atcgacaatg gttattggta atttggcaga aagtaatgaa ctaactgcct
     3361 ataaagcatc gggaatttcc ttaattcgcg ttatgtttgg attgatattt tcagctttgt
     3421 taatggcaat ttttgctttc tatttttcaa atagaatctc tccttatgtc aatctaaaat
     3481 tttatacttt gttgtatgat atacgaaagc aaaaaccagc attggacatt actgaaggtg
     3541 ttttttatcg aggcataagt aattttgcta ttaaagtaaa aaagaaagat aaagataata
     3601 aaacattgta tgacattata gtatatgatc ataccaattc tgataacaac agcattgtaa
     3661 ctgcaaaaaa aggtgaagtt ttattaagta atactggtca atatttgatt ttaaatctat
     3721 atgaaggtag tcaatatcaa ttcaagccca atattaataa acaagatggt tctgtgaaag
     3781 agatgactca attatcattt aaaaaatggc gaaaaatatt tgatttgaaa gaatttaaaa
     3841 tggagcgaac agatgaaagt ttgttcaaat ccaattatgc aatgtacaat tctgctcaat
     3901 tgagattatt aagcgattct gttcaaatgg agctaaacaa atcagaaagc aatataaatc
     3961 agactattaa aaataatttt gttcctttta agcaagatga tataagccta agtgaaaatg
     4021 atataatgct taaaaatata agtatcaatt tagatagtat ttatgcaaga aatattgatg
     4081 tccgaggtac aactatggca ttaggtttag taaatggcgt aatagccaat attgagaact
     4141 ccagagatga tattaaatgg aagcttagtg atatggctca atatcaaata gaattgtatc
     4201 gaaaattcag tttgtcattt gcatgtattg tcttgatgtt aattggtgct gtaatgggtg
     4261 caattgtacg aaaaggtggt tttggagttc ctgtattaat tgcggtttta tattttgttg
     4321 cctttcactt ttttaatgtg ataggggaaa aattagccaa aaatgaagta ttgccagttg
     4381 caattggcat gtggctttct gctatcgtat tatttcctat tgcattattt ttaacctata
     4441 aagctatgaa tgactctgta attttcagaa ttgaaagtat tcaaaagaca tttcatatat
     4501 taaagaaaaa actacctaaa gcaaaatgat tatttttaat atactcttat tattttcact
     4561 ttttgcttta gtttatagct atttcttata tccattgcta ttaaaaataa tatcaagttt
     4621 taatttaggc aaacaatcaa tatctattga taatcatata gatataaaat acaaagatat
     4681 aaatattatt attccagtac ataatgaaga aagtataatt ggctcgaaac taaaatctat
     4741 cgtaaatcaa aattatccaa aggagaaaat aaaaatttat attggcttag atgcttgtac
     4801 tgataattca attaaagaga ttgaatatat tgatagtcaa ttaaatataa ttaaatttga
     4861 aagtaaaact agaataggaa aacctaagct tttaaatcat ttatttgaac taattcaaga
     4921 tgaagaatct ataattttag taacagatgc tgatattctt ttagaaacta atactttaat
     4981 tgatatatca aaatcatttg ataatgaatt aaatatactt acagatttaa agttaataaa
     5041 taagaacatc gaagcaatag aggaaaattt atatttagat ttggaaaaca ctattaagtc
     5101 atgtgagtct aaaaactttc atatcttcca aggtgtttct ggtgcatgtt atgccatcaa
     5161 acgaaaatac ttcaagccaa ttcctgaaaa ctttctagta gatgattttt atatttcaat
     5221 acaggctttg atgcaacaac catttgctac ttttttagaa aatagctttg tttatgaaaa
     5281 tcgtccaaaa gatatgtcat tagaatttaa aaggaaaatt agaattgcta ctggaaattt
     5341 tcaaaactta tatttttttg gcacaaaact cttaaatcca atttctgcaa taggctttac
     5401 ttttatttcg cacaaattat tcaggtggct tacgcctttt tttatgttgt atatttctat
     5461 ctatttactc ataaattata catttattat tttagcctta acactcattt tgctatctat
     5521 tgttttcttt ttaagtattt ttgaagttaa gaaattcgtt agaattccat tctattttat
     5581 agcaatgcaa attgccgttt tgattgggtt ttatcgcttt ctaaaaggtg taaaaacaaa
     5641 tatttggcaa cctacaaaca gaatttaata tgttagatac aatcgaatcc gcattagagg
     5701 atataaaaaa aggaaaatta attattgttg ttgatgatga agaccgcgaa aatgaaggcg
     5761 attttgttac cgcagcaaga aatgtaacgc cagaaattat caatttcatg gcaactcacg
     5821 gtcgaggctt aatctgtgct cctattattg aagatgaatg tgatagatta ggttttgaga
     5881 tgatggtcaa tagtaattca tctcattttg atacaccttt tacggtttct attgatttaa
     5941 ttggcaatgg ttgtactact ggaatttcaa ctgctgatag agctaaaacc atacaagcat
     6001 tggttgaccc aaatagtaaa ccatcagatt ttggaaaacc aggacacatt tttcctttaa
     6061 aagcaaaaaa agaaggcgtt ttaagacgaa caggtcatac cgaagcaaca atagatttag
     6121 caagattggc aggatttgaa cctgctggtg tgttggtaga aattatgaat gaggacggca
     6181 ctatggctag aacacctgaa ttattagaaa ttgcaaaacg ttttgattta aaaattattt
     6241 ctataaaaga cttggttgaa tatcgtttaa aacacgaaaa acaaattgaa cgtgttgtag
     6301 aagtgaatat gcctacaaaa tatggagatt ttaaattgac ggcatacaaa gatattcaaa
     6361 ctggtgagga acattttgca ttaacaaaag gcacttggga aaaagatgag cctgttttag
     6421 ttagagtaca ctcttcttgt attactggag atattttagg ttcattgaga tgtgattgtg
     6481 gtgaccaatt acacggtgcg atggaaatgg ttcaagaagc aggaaaaggc gttatcttgt
     6541 atatgatgca agaaggaaga ggtattggat tttttaataa attgagagct tataaattgc
     6601 aagaagaagg catggatacc gttgatgcta atttagcttt aggtttcaaa gctgaccaac
     6661 gtgattttgg tattggtgct caaattttaa gagatttgaa tatttctaaa ttaaaattaa
     6721 ttactaataa cccaaaaaag cgtgctgctc ttggtggtta cggtttagaa attgtagaaa
     6781 atattcccat caaaatcgca ccaaataaac ataacaaaaa gtatttagat accaaacgtg
     6841 ataaaatggg acacgaactt aggtaactag acaaatagta aagcattaga aaaaacattt
     6901 tgaggaacaa agtttgctta attgataaaa acagataaac acttattact ttatacttaa
     6961 aatttaaaaa atgagtaaaa ttttaatgtt agataattat gattcgttta catacaatat
     7021 tgtacattat gtagaatcat ttgattttaa agttgatgtg tttagaaatg ataaaattgg
     7081 cattgaagct atcaatcaat atgacaaaat aattttatct ccaggtcctg gaattcctga
     7141 agaagcaggt attttattag atgttataaa aacttatgct ccaacgaagt gtattttagg
     7201 tatttgttta ggacatcaag cgattgggca agtttttggt gcagagttgt ataatgtagg
     7261 acaagttata catgggaaaa ctagaaacac tattatcaaa gagcaagata taatttttga
     7321 tggtttggac actaattttg aaagtggtcg ttaccattct tgggcaatta aaaatattca
     7381 agcacctttg caagttacag ccattgatga tgacggcatt gtacaagcta ttagacatca
     7441 agaatataat gtaagaggtg ttcaatttca tcctgaaagt gtaatgacac ccaacggaaa
     7501 aaaaatcatt gaaaattggc ttttcaaatg ctaatttata tctttatcca tcctatacat
     7561 tggggcatta gctcagctgg
//
LOCUS       DAOQCW010000119         7564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_3864_length_7564_cov_4.913970, whole genome shotgun sequence.
ACCESSION   DAOQCW010000119 DAOQCW010000000
VERSION     DAOQCW010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7564
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3864_length_7564_cov_4.913970"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="PLS16_09700"
     CDS             complement(<1..1070)
                     /locus_tag="PLS16_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61397.1"
                     /translation="MTDINLIKRSEQPTPLTHEQVDSNWQTIEDAVNELNNTIDNSRY
                     IISGFPIWSGVGLEYLYPHINYVWNGVELTAPQGSITLDTSDVSFDRFDLIVLSEDGT
                     VSVIKGTASDNPIIPTLPNDRLLLLTINVKAGMTEPNVISEIIYNENSEWTTSISLKN
                     NPNIPNDLGSIDFDSTNEVKVGAKSIHWNTNEKKIAIFKPASLVNPTDWTTLSMWIML
                     KEPINDGRRFEFQFYKTAGNAIGNYIDIKTKGLNQTLLNTWQLITIPIADFNIPNGEQ
                     IERLRATFTLGSPNVVRDLYIDYIRLTTDVEQVIEPLKEFNVSVNGQLIGAVESINFI
                     EDENIEINGTLNDKTADISIKA"
     gene            complement(1082..1435)
                     /locus_tag="PLS16_09705"
     CDS             complement(1082..1435)
                     /locus_tag="PLS16_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61398.1"
                     /translation="MNLGCVALCDTIQLPFSLNVTGTHTFIFSFILDVGISREVEVEN
                     INNVSLEVAELDLPEIGCLRFQIIQPNGVAFSLVEDNKAYNCFEFDMQIEDCESGGCT
                     ETYVDCEYWQELYSL"
     gene            complement(1716..2210)
                     /locus_tag="PLS16_09710"
     CDS             complement(1716..2210)
                     /locus_tag="PLS16_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61399.1"
                     /translation="MIVDKSKILDLFKDTGYTITGIGVFRSVNNVQMLIDTSDDNKPI
                     GLEDICNTGYIRYLNDTKISNVKSYSCTRSIGMATDLILVLLHNKININNTSLRLTSL
                     LLKCDDIDILSVDINKENIEKSEGIKNNPYELIKITFRYKEEYNEGDCVLENDCETLN
                     INEC"
     gene            complement(2200..2688)
                     /locus_tag="PLS16_09715"
     CDS             complement(2200..2688)
                     /locus_tag="PLS16_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61400.1"
                     /translation="MAESNAIVVSNNLLGYRITDDLIELAIGGALDEVHTHILDRIFN
                     QNKDVNGGGFGGYSESYAKYRKSLGLQSGTKDLQLSDRLRNSIYVDKEKRAIMLSNQT
                     DMQLPARNGKLKSSNITQSAKARMNEKYLGQDIFSADKFEREVAIKVFKDIIFEKLGN
                     DS"
     gene            complement(2815..3726)
                     /locus_tag="PLS16_09720"
     CDS             complement(2815..3726)
                     /locus_tag="PLS16_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61401.1"
                     /translation="MSCFDKLISIRGLCENDTALYYLDDYDVSLMLASKTADERYVSG
                     KRLIETKIGQAWRSLFKDITFDGMKVNSIIEEFNFRGSENATTQSSGVSFSLADGCDL
                     AMIFVSRIILDVETGGTAVVKINNDGVISNIFSGTLESNSQMIIDLNQFVADDFRLYI
                     ESEDVVLISKKVNRHNGFIQANSEVTNGFNVEMQLRCTTEKYLCKYADLLCIAVVYKA
                     LALIWNEVRDTNRLNNLVNLKREDAVTKMAFYDSTFNLLKYDITASVNYAPKGLYQLE
                     LEKLNIPTPNCRCCLSCKSDSYIISLP"
     gene            complement(3803..4912)
                     /locus_tag="PLS16_09725"
     CDS             complement(3803..4912)
                     /locus_tag="PLS16_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61402.1"
                     /translation="MATVINKTGITPCLLLQNEINKNFGALASDAMKPNVGLYNALIS
                     DANRVGVSINEDVNNGQARPTNTSKRIVQLRYVKPTCTDESEDEEVGECVTPDRGLAS
                     TGYANVEVNDFVSFGFELNDSEFDEMCASRTTVYTENLMSEYYKALKRLDKKLLAKVL
                     PTFGKYPNGDSSLTETIDVPFINANGTPNIGAFAKIQAMYRQLGYPANPIFVGDSDLL
                     SLEYMQKYLGQGANGINLANFTFNNFFFDAQVNAEIGDAPTDKRLITFAPGTYQFVNF
                     LDTKRREVEKPMLLTINGEEVTRFEKENKVMSLEDGDWDFYYTYDCGKHNFNWQKRFD
                     LFMLPSDSVCNYPSLLFGVDCGDLSCDTFNSNGSY"
     gene            complement(5011..5841)
                     /locus_tag="PLS16_09730"
     CDS             complement(5011..5841)
                     /locus_tag="PLS16_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61403.1"
                     /translation="MKINKKRMEKSKELLKKFGFNAEKVEDENFDIEKAFADFQKKQE
                     DLFMQRQDLLEPILTEKVKTANIISAKKTKKAINEFFGLGKTEKELNDIEIQDLLDAG
                     KTGLNLEVSKDVKEHQAKIVELQNQIAQIQEAKAKELEDVKNEYSKKEKQAKISNIIN
                     SELTKDVDFLIGKDKVVKLFESSAKDAGFEFDIDDENNFIIKKGNYRAMTSDNTKFAD
                     LSNLRETLIGDLIKKSNGGGGQPPHNGGGADHKTIYPENLPKHMRERLEAMNQKASAI
                     "
     gene            complement(5807..6082)
                     /locus_tag="PLS16_09735"
     CDS             complement(5807..6082)
                     /locus_tag="PLS16_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61404.1"
                     /translation="MILINIDNGNVINASKFSERYIEKLLSTKKFIKESDKFEVSTDQ
                     GVEILEQERIRLVEWEEKLMNKENELNELAKSLELINENKQKKNGKK"
     gene            complement(6142..7011)
                     /locus_tag="PLS16_09740"
     CDS             complement(6142..7011)
                     /locus_tag="PLS16_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61405.1"
                     /translation="MNEVDYIIDLVEKNKEKLIAINKEFYQALVDELSIFIHGGYYDY
                     TDEALALIGGIIVGAFQNSGYFKNAKNFQKELSKIVDRTKLFYKDDKGMTFKKLNENN
                     VRIQRAIDDVMFDLTGKGFEVNVMRKIENKVFSDIVLKREVKESFKELKQITIGDESL
                     VNNQNGLILQENLLRLNGTANDIIRKENKLDTLKWIGSLVEESRPMCTHIRENYPNGM
                     TISEVNKVLNEFIPNGEPSDSQVKVINAKGKEVYRAKGSGLIEGTDITNITENRGGYN
                     CRHNVKWVRFVRP"
     gene            complement(7004..>7564)
                     /locus_tag="PLS16_09745"
     CDS             complement(7004..>7564)
                     /locus_tag="PLS16_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61406.1"
                     /translation="NQSGESKRYDSQQRVDLVSGILFDLFKLYENILNSIAEYRGGSN
                     DVNISLPEDLDIKNSADYIVEIAQAKSSGAPYLILVELTKRYLLKNFGASAVNEFVIN
                     ELAKIDKIFAYGSSELVQAKASLGSDLTNEDIILHHQGFQIMIDLFNEGKITKDSSTD
                     EINAIVRKVINETFNLSKPIISLEDE"
BASE COUNT         2336 a         1529 c          907 g         2792 t
ORIGIN      
        1 ttagctttaa tacttatatc agcagtttta tcatttaaag ttccattaat ctctatattt
       61 tcatcttcaa tgaaattaat actttcaact gctccaatta attgaccatt tacacttaca
      121 ttaaattctt taagcggttc aattacttgc tcaacatctg ttgttagtct aatataatca
      181 atatacaaat ctcttacaac atttggacta cctaaagtaa atgtagctct taatctttct
      241 atctgttctc catttggaat attaaaatca gctattggaa tagttataag ttgccaagta
      301 tttaaaagtg tttggttcaa tcctttggtt ttaatatcaa tataatttcc aattgcattt
      361 ccagcagttt tataaaattg aaactcaaat cttctaccat cattaattgg ttctttgagc
      421 ataatccaca ttgataatgt tgtccaatca gttggattaa ctaaggaagc tggtttaaaa
      481 atagcaattt tcttttcatt agtattccaa tgaatagatt ttgcaccaac ttttacttca
      541 tttgtactat caaaatcaat agaacctaaa tcatttggaa tatttggatt attctttaac
      601 gaaatagaag tagtccattc tgaattttca ttgtaaatta tttctgaaat aacatttggt
      661 tctgtcattc cagctttaac attaatagtt aataataata atctatcatt cggtaaagtt
      721 gggataattg gattatctga tgctgttcct ttaataactg aaactgtacc atcttcagat
      781 aaaactatca aatcaaatct atcaaaacta acatctgatg tgtcaagtgt aatgcttcct
      841 tgtggtgctg tcaattctac accattccat acgtaattga tatgtgggta caagtattcc
      901 aacccaacac cgctccaaat aggaaagcct gaaataatgt atcttgaatt gtcaatagta
      961 ttattcaact cattaacagc atcttcaata gtttgccaat tactatccac ttgttcatgt
     1021 gttaatggcg ttggctgttc gcttcttttt attaagttta tatctgtcat tttatttttt
     1081 tttataatga atataattct tgccagtatt cgcaatctac ataagtttct gtacaaccac
     1141 cactttcgca atcttcaatt tgcatatcaa attcaaagca attataagcc ttattatctt
     1201 caactaaaga aaacgcaaca ccattaggct gtataatctg aaatctcaaa caaccaattt
     1261 caggcaaatc aagttccgca acttctaagc taacattatt aatattttcg acctccacct
     1321 ctcggcttat tcctacatct aaaataaagc taaatataaa cgtgtgagtg cctgtaacat
     1381 tcaatgaaaa tggtaattga atagtgtcgc aaagtgctac acaccctaaa ttcattatat
     1441 tttcgcaaca tctattcatt tgattatgaa ttttaacgct gtattcgcaa ttccaaatat
     1501 agccaaaata ttaaataaga taggcacaaa attaaacaac aaaatcatca acaccgccac
     1561 ccacacattt gtacatatca agcacccccc taatggctta aatataggct tattttcaat
     1621 gaatttgccg tacttttcaa gtatcatacc ctcttgtatt atatcatcaa aaaagttcgt
     1681 aaaacaagct gaaataaagc cgacaaaaag tacatctaac attcgtttat atttaatgtt
     1741 tcacaatcat tttccaaaac acaatcgccc tcgttatatt cttctttata tctgaacgta
     1801 attttgatta attcgtaagg attattttta ataccctccg atttctctat attttccttg
     1861 ttaatatcaa cgctcaatat gtcaatatca tcacacttca atagcaatga tgttagcctt
     1921 aacgatgtat tatttatatt aattttatta tgcaacaaca ccaatatcaa atccgttgcc
     1981 attcctatac ttcttgtaca actataactc tttacattgc taattttggt atcattcaaa
     2041 taacgaatgt agccagtatt gcaaatatct tcaagtccaa taggcttatt atcatcgctt
     2101 gtatcaatca gcatctgcac gttattaacg cttcggaata cccctatgcc agttatggta
     2161 tagccagtat ctttaaataa gtctaatatt ttacttttat caactatcat ttcctaactt
     2221 ttcaaatata atatctttaa atacttttat agctacttcc ctttcaaact tatcagccga
     2281 aaatatatct tgtcccaaat atttttcgtt cattcgagcc ttagcagatt gcgtgatatt
     2341 actacttttt aacttaccat tcctcgctgg gagttgcata tcagtttggt tgctcaacat
     2401 aatagcacgt ttctctttat cgacataaat actattcctt agcctgtccg acaactgcaa
     2461 atcttttgtg ccactttgca atcctaatga tttgcgatac ttagcataac tttcagaata
     2521 ccctccaaaa cctccaccat ttacatcttt attttggtta aatattctat ctaaaatatg
     2581 ggtatgcact tcatctaaag cacctccaat agccagttct attaggtcat ctgttattcg
     2641 atagcctaat agattattac tcactactat tgcattactt tcagccattt ttctttataa
     2701 aagtaatatt ttttggctta atagcattgg ttactattgt aacttttttt gttccataat
     2761 tcttagtagt ctgtactttt ttaccgcatt ttaaacaact cataatattt ttttttaagg
     2821 taatgaaata atataactat cactcttgca actcaaacag catctacaat taggtgttgg
     2881 tatgtttaat ttttccaact ccaattgata taatcctttt ggtgcatagt ttacactcgc
     2941 tgtaatatca tattttaaca aattaaaagt actgtcataa aaagccattt ttgttactgc
     3001 gtcttcacgc tttaaattta ctaaattatt caacctattc gtatctctaa cttcgttcca
     3061 aatcaaagcc aaagccttgt aaacaactgc tatacatagc aaatccgcat acttgcaaag
     3121 gtatttttca gtcgtgcaac gcagttgcat ttctacatta aaaccattcg taacctctga
     3181 atttgcttga ataaagccat tatgcctatt tactttcttg ctaattagta caacatcttc
     3241 actttcaata taaagcctaa aatcgtccgc tacaaattgg tttaagtcga ttatcatttg
     3301 tgagttactt tctaaagtgc cactaaatat attactaata actccatcgt tatttatttt
     3361 tactactgca gttcctccag tttcaacgtc taaaataatt cttgaaacga aaatcatcgc
     3421 caaatcgcac ccatccgcta aactaaacga aacacctgaa ctttgagtag ttgcattttc
     3481 tgaacctcta aaattaaatt cttcaataat gctattcact ttcattccat cgaaagtaat
     3541 atctttaaac agcgacctcc acgcttgacc tatcttagtt tcaatcaatc gcttaccact
     3601 tacatatctt tcgtctgccg tcttagatgc caacattaaa gacacatcgt aatcgtctaa
     3661 atagtatagt gctgtgtcgt tttcgcacaa cccacggatg gaaattaatt tatcaaaaca
     3721 gctcatatat atttatttaa aaaagggcgg tttcagcaca aaacctccac cgcctaaaaa
     3781 aaacaaaaaa aagtacctac attcagtaag aaccattgct gttgaacgtg tcacacgaca
     3841 gatccccaca atcaacacca aataacaatg atggatagtt acatacagaa tcgcttggca
     3901 acataaataa atcaaatctc ttttgccagt taaagttatg tttaccacaa tcgtaagtgt
     3961 agtagaaatc ccaatctcca tcttctaatg acattacctt attttctttt tcaaagcgag
     4021 taacttcttc gccattgata gttaatagca ttggtttttc tacctcacgt ctttttgtgt
     4081 ctaagaaatt cacaaattgg taagtaccgg gagcaaacgt aatcaaacgc ttgtctgtcg
     4141 gtgcgtctcc aatctcagca ttaacttgag catcaaaaaa gaaattgtta aatgtaaaat
     4201 tagcaaggtt aatgccatta gcaccttgtc ctaaatattt ttgcatatat tccaacgaca
     4261 gcaagtcgct atctcctaca aagataggat tagcaggata gcctaattga cgatacatag
     4321 cctgtatctt cgcaaatgca ccgatatttg gcgtaccatt tgcattaatg aaaggcacat
     4381 caatagtttc agttaaagaa ctatcgccat taggatattt gccaaatgtc ggcaacacct
     4441 tagctaataa tttcttgtcc aaacgtttca aagccttgta gtactcggac attaagtttt
     4501 ccgtgtaaac ggtagttcta cttgcacaca tttcgtcaaa ctccgaatca tttaattcaa
     4561 acccaaaaga cacgaaatca tttacttcaa cattagcata accagtagat gctaaccctc
     4621 tatcaggcgt tacgcactcg ccgacctctt catcttcaga ttcgtctgta caagttggtt
     4681 ttacatagcg taattgtacg atacgtttag acgtatttgt tggtcttgct tgaccattat
     4741 taacgtcttc attgattgac acacctactc tatttgcatc agatataagt gcattgtaaa
     4801 gccccacatt tggcttcatt gcatcggaag ccaaagctcc gaagtttttg tttatctcat
     4861 tttgtaagag taaacacggc gtaatgcctg ttttgttaat tactgttgcc atattatttt
     4921 aatttataat gtttaaaatt tttactatgg cattttaaaa gtatgctaaa ctcaataagt
     4981 tgcttaaaag tggtgcaacc cccacagtta ttatattgca gaagcctttt ggttcattgc
     5041 ttctaaacgc tcacgcatat gttttggcag attttcagga taaatagttt tatggtctgc
     5101 accaccaccg ttatgtggag gttgtccacc accaccattt gacttcttaa ttaaatcgcc
     5161 aatcaaagtt tcccttagat tgctcaaatc ggcaaactta gtattatcag atgtcatcgc
     5221 tcgatagttg ccctttttga taataaagtt attttcgtca tcaatatcaa attcaaaacc
     5281 cgcatctttt gccgaacttt caaacaattt gacaacctta tctttaccga taaggaaatc
     5341 aacatcttta gtaagttccg aattaatgat attgctaatc ttagcctgtt tttctttttt
     5401 tgagtattca tttttcacat cttcaagttc tttagctttc gcttcttgaa tttgtgcaat
     5461 ttgattttga agctctacta tcttagcctg atgttccttt acatcttttg agacttctaa
     5521 attcaaacca gtttttccag catcaagcaa atcttgaatt tcaatatcgt taagctcttt
     5581 ttctgttttt cctaacccga aaaactcgtt aatcgctttt tttgttttct ttgcagaaat
     5641 aatatttgca gtcttaactt tctccgttaa aataggctct aagaggtctt gtctttgcat
     5701 aaacaaatct tcttgctttt tctgaaagtc tgcaaatgcc ttttcaatat caaaattttc
     5761 atcttcgact ttctcggcat taaaaccgaa tttttttaat agttccttac ttttttccat
     5821 tctttttttg tttattttca tttattaatt ctaacgattt tgcaagttca tttagttcat
     5881 tttctttatt catcaatttt tcctcccact ctaccagtct tattctttcc tgctctaaaa
     5941 tctcaacacc ttgatccgtt gatacctcga acttatcact ttcttttata aattttttag
     6001 tagatagtaa tttttcgata tacctttcag aaaacttcga ggcattaatt acgttaccat
     6061 tatcaatatt aattaatatc ataactcaaa aataatgata gtgcttaaaa taaagttggt
     6121 aaattttgta ctttgttttt tttaaggtct tacaaaccta acccacttta cattatgtct
     6181 gcaattataa ccacctctat tctcggttat attggttatg tccgtacctt caattaatcc
     6241 tgacccttta gctcgataca cttccttgcc tttggcattt attaccttta cttggctgtc
     6301 gcttggttct ccgtttggaa taaactcatt tagaacttta ttaacttctg aaatagtcat
     6361 accatttggg taattttccc taatatgcgt acacatcgga cgactttcct ccactaacga
     6421 ccctatccat tttaaagtat ctaatttatt ttcttttcta attatatcat ttgctgtacc
     6481 attcaaacgc aaaagatttt cttgcaatat taagccattt tgattattta caagactttc
     6541 atcgccaata gttatctgct ttaattcttt aaaactttct tttacctcac gctttaaaac
     6601 aatatctgaa aatactttat tttctatctt tctcattaca tttacttcaa agcctttgcc
     6661 agttaaatca aacattacat cgtcaattgc tctttgtatt cttacgttat tttcattcag
     6721 ttttttaaag gtcattcctt tatcgtcctt gtaaaatagc ttagttctat caactatttt
     6781 tgataattct ttttgaaagt tttttgcatt tttaaaataa cctgaatttt gaaaagcacc
     6841 aacaataata ccaccgatta atgctaatgc ttcgtctgta taatcataat agccaccgtg
     6901 aatgaaaatc gataattcat caaccaatgc ttgatagaac tctttgttta tcgcaattaa
     6961 tttttctttg tttttctcaa ctaaatcaat gatataatca acttcattca tcttctaaag
     7021 atataatagg ctttgataaa ttaaaggttt cgttaattac ttttctaaca atagcattta
     7081 tttcatctgt actactatct tttgtgattt tgccctcgtt aaataagtct atcattattt
     7141 gaaaaccttg atggtggagg attatatcct cgttcgtcaa atcactacct aaacttgctt
     7201 ttgcttgtac caattccgaa gaaccataag caaatatctt atcaatcttt gcaagttcat
     7261 taattacaaa ttcattaacc gcactcgcac caaaattttt taataaatat cttttcgtca
     7321 attctaccaa aataagataa ggcgccccac tactctttgc ctgtgcaatt tcgacaatat
     7381 aatcagcact attctttata tctaaatctt caggtaagct aatgtttaca tcatttgaac
     7441 ctccacgata ttccgcaatt gaatttaaaa tattttcgta taacttaaat aaatcaaaca
     7501 aaatgccact taccaagtca acccgctgtt gactatcata cctcttactc tctccacttt
     7561 gatt
//
LOCUS       DAOQCW010000120         7204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_4161_length_7204_cov_5.976500, whole genome shotgun sequence.
ACCESSION   DAOQCW010000120 DAOQCW010000000
VERSION     DAOQCW010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7204
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4161_length_7204_cov_5.976500"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="PLS16_09750"
     CDS             <1..918
                     /locus_tag="PLS16_09750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNJ61407.1"
                     /translation="QTYNGDYVSVGYGTYTLDPSNLPQLDHVSLVEYWNINSSITPSA
                     NDDALVKLFWRKHSKVSTSSTDWDNLRVVHFDGTDWNTEDNSASSSNVSGASINWGYV
                     ISPNYITDYSPYTLGTISKANPLPIELKSFTAKCIENEKAQLNWITASERNTANFVVE
                     KSLDAITFYPIGTIQAAGNSISESHYQFIDELPNDRAYYRLKEIDIDGKESIHPVIYT
                     KCGKTINGIQVYYAGNNQIHADIQTNKTEEYIFNLYQTDGKLIFHDSKVILEGKRKVI
                     LSQNQLAKGIYLLQVINGENMQTKKIIVD"
     gene            984..>1738
                     /gene="lpxA"
                     /locus_tag="PLS16_09755"
     CDS             984..>1738
                     /gene="lpxA"
                     /locus_tag="PLS16_09755"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNJ61408.1"
                     /translation="MKDIHPNAQIGKDTVISNFTTIYDNVIIGDNCWIGANVTIFSGT
                     RIGNNCKIFPGAVIGAEPQDLKFNGEYSTVEIGDYVTIRECATINRGTAALGTTKVGD
                     YCLIMAYVHIAHDCIVGNNCILANSTNLAGHVIIDDHVYFGGMSGIHQFVRVGRHAFI
                     SGGTMLGKDVPPYCMVIGTPPQYAGINTVGLKRHQFAQNDIHHIQDIYRILFGSGLNT
                     KQAIEKISTEIKETEIRNHIINYICLSERGIIKG"
     assembly_gap    1739..1838
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1839..2134
                     /locus_tag="PLS16_09760"
     CDS             <1839..2134
                     /locus_tag="PLS16_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNJ61409.1"
                     /translation="ESHYLVAHNMAFDEKIMGAEFLRKDIENNIIYRKKICTMLASRN
                     FCAIEGPYGYKWPKLAELYYKLFQTNFDNAHDASADISATAKCFWELRKKGII"
     gene            2205..4244
                     /locus_tag="PLS16_09765"
     CDS             2205..4244
                     /locus_tag="PLS16_09765"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNJ61410.1"
                     /translation="MQQQQLPWLKLLLPLLLGIVCSKWIAVEIHWAFLLVLLSFYFLS
                     NYFYRKYNKSWLYILSFISLIIFGLAYTQRFQLAFQKHHFSKFEGDYAIGIVREPIEN
                     KTNSYQTTIEIKNIIQEHKIFHTKGKLLLYIQKDSLKNPLEIGDEILLHLKSNEIEAP
                     KNLKQFDYKTYLSYNDIYHQAYIKNFEYKILAKHQRYFIKRKSDEISKYAQSLLQKYI
                     PNKENFNIADGIFLGHKSEMDIEQYNTFAYLGIVHILSVSGLHVGIIYLLISFFLSFI
                     PNKNKTIYILKFLTAFLLIWLFAFVVGLSPAVVRSALLFSILHFGRILKEDTIPLNIL
                     LGAAFVQLLINPLVLYNIGFQLSYLAMLGLYLFYKPIYNLLYSENKFINWFAQLCSAS
                     IAAQIFTLPFAIYYFGNFPTYFLIANIFAIPLSTLILWFCVALLPLQFIPLLAKLTGQ
                     LISYLIDVFIYLSKLLLLLPYAKISTLFINEWQLILCCLAIAIFTIYIFSQKIKYALY
                     ALSIVVLIIISAYYFQYQNYKQDKLVFYAVNKNLVYGFHQKSNVTIYSKDTLSLKDFN
                     FNVSNTCRYFRNQTINNNLIVQDLQLIKSKSIYFLHQNNIQKSYQAPLSIDILVLTDN
                     IYLDIEKLVQNYRFKQLILSSDLDYKHQKIYTKLLNEAGISFTDLKQQAYIDYNI"
     gene            4305..5063
                     /locus_tag="PLS16_09770"
     CDS             4305..5063
                     /locus_tag="PLS16_09770"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNJ61411.1"
                     /translation="MEFIKINKKENYAILQMDRGKSNPINQQFVDEFIEALNQLMADN
                     EVLGVVVAGKENFFSAGLDIPELYSYDEAQFDVFWKKFLQMILQLSKFNKPMVAAITG
                     HAPAGGCIIANGCDYRVMADGNYKIGLNEIPVGIVVPKGVYAQYSMWIGTRNAYQYLM
                     EGKLYAPKHAQEIGLIDEVVPLENVLERAETKLKQYLQYEQSGWRLTKTQLRQSVVDT
                     MESLTDKEMVMFQKQWWSESVRAILGAFIDKLKK"
     gene            complement(5064..5846)
                     /gene="kdsA"
                     /locus_tag="PLS16_09775"
     CDS             complement(5064..5846)
                     /gene="kdsA"
                     /locus_tag="PLS16_09775"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNJ61412.1"
                     /translation="MFKLSNFFLIAGPCVVENQEIGFEVAETLVSITQKLNIPFYFKA
                     SFKKANRTSIHSFSTIGETKALQILADIKSKYQVQILTDVHESSDIEKVKDIVDVIQI
                     PAFLCRQTDLILAAAKTEKIINIKKGQFANAIQMQFAADKVRSVGNDNIWLTERGNSF
                     GYQDLVVDFRNIPLLKTNHCKAIIDITHSIQQPNSTEGKTLGTPEFIELIAKCGIVAG
                     ADGLFLETHPNPSNALSDGSNMIALNKVENLLSKLQKIAAVL"
     gene            complement(5907..6797)
                     /gene="dapA"
                     /locus_tag="PLS16_09780"
     CDS             complement(5907..6797)
                     /gene="dapA"
                     /locus_tag="PLS16_09780"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNJ61413.1"
                     /translation="MSSINLRGVGVALVTPFNEQGEIDFNQYEQLCKHVIANHVDYVV
                     VLGTTGEVATMSDEERQDMIRATVNNVAGKVPVIAGFGGNYTQKIVADLNKFDLSGVD
                     AILSVSPYYNKPTQDGLYEHYKTIALSTELPVILYNVPGRTSSNIKASTTLKLAENFK
                     NIIAIKEATSDWSQIIELAHHKPDGFYLISGNDDIIIPQISLGYDGVISVIANATPKI
                     FSEMVHQALDGNYADARQTVFALDDLVNMLFEQGNPGGVKCALHHLGICSETVRLPLM
                     PVNEDLSNRIKSKLSDLGLL"
     gene            7092..7165
                     /locus_tag="PLS16_09785"
     tRNA            7092..7165
                     /locus_tag="PLS16_09785"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7126..7128,aa:Asp,seq:gtc)
BASE COUNT         2509 a         1029 c         1095 g         2471 t
ORIGIN      
        1 caaacgtata atggcgatta tgtgagtgtt ggctatggaa catacacttt agatccttct
       61 aatttaccac aattagacca tgttagtttg gtagaatatt ggaatatcaa ctcctcgata
      121 acaccatcag ccaatgacga tgctttagtg aaattatttt ggagaaaaca tagtaaagta
      181 agtacaagct ctaccgattg ggataattta cgtgtggtgc attttgatgg tacagattgg
      241 aatacagagg acaactcagc ttcttcaagt aatgtgtcag gagcaagtat aaattggggt
      301 tatgtgattt cacctaatta tattacagat tattcgccat atactttggg aacgatttct
      361 aaagcaaatc ctcttccaat agaacttaaa tcttttactg ctaaatgtat tgaaaatgaa
      421 aaagcacaac tcaattggat tactgcttct gaaagaaata ctgctaattt cgttgttgaa
      481 aaatctttag atgctattac tttttatcca attggaacaa ttcaggcagc tggaaattct
      541 atttcggaat cacattatca gtttatagat gaattgccaa atgacagagc atattataga
      601 ttaaaagaaa tagatataga tggcaaagaa agtattcatc cagttatata tactaaatgt
      661 ggtaagacta taaacggcat tcaagtttat tatgcaggta ataatcaaat tcatgcagat
      721 attcaaacta ataaaacaga agaatatata ttcaatttat atcaaactga tggtaaattg
      781 attttccatg attcaaaagt tatattggaa ggcaaaagaa aagttattct ttcgcaaaat
      841 caattagcaa aaggtatata tctattgcaa gtaattaatg gcgagaatat gcaaacaaaa
      901 aagataattg tagattaaaa gaaaatcaca aaatacattc taatatattt tacgacttta
      961 gtaattatat tatatttgta ggaatgaagg acatacaccc aaatgcacaa attggaaagg
     1021 atactgtaat tagtaatttt acgacgattt acgacaatgt gataattggg gataattgtt
     1081 ggataggtgc taatgttacc attttttcag gcactagaat tggcaataat tgtaaaattt
     1141 ttccaggagc tgtaatcggt gcagagccac aagatttaaa atttaatggc gaatattcta
     1201 ctgttgaaat tggagattat gttacgattc gagaatgtgc caccatcaat agaggaactg
     1261 ctgcattagg aacgacaaaa gtaggcgact attgtttgat tatggcttat gttcatattg
     1321 ctcacgattg tattgttggc aacaattgta ttttagcaaa ttctaccaat ctagctgggc
     1381 atgtaattat cgatgaccat gtttattttg gtggaatgag tggcatacat caatttgtta
     1441 gagttggaag acatgctttt atctctggtg gcacgatgtt ggggaaagat gttccgcctt
     1501 attgtatggt aataggcaca cctccacaat atgctggcat taatacggtt ggattaaaac
     1561 ggcatcaatt tgctcaaaat gatattcacc acattcaaga tatttacaga atactatttg
     1621 gcagtggact aaataccaaa caagccatag aaaaaattag tactgagata aaggaaacag
     1681 aaattagaaa tcatattatt aattatattt gtctttcaga acgtggaatt ataaaaggnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnta gaatcacatt atctagttgc
     1861 tcacaacatg gcatttgatg aaaaaattat gggtgctgag tttttaagaa aagatattga
     1921 aaataacata atttatagaa agaaaatttg tacaatgtta gcatcaagaa atttctgtgc
     1981 gattgaaggt ccttatggtt acaaatggcc aaagttggca gaactttatt acaaactttt
     2041 tcaaacaaat ttcgacaatg cccatgacgc ttcggctgac atctcagcaa ctgcaaaatg
     2101 cttttgggaa ctgagaaaaa aaggtataat ttaattctaa ttaaaaagca aaagtttttt
     2161 caaaatagat tttagtatat ttaacttagt aaacttgtga agttatgcaa caacaacaat
     2221 taccttggtt aaaactatta ttgcctttgc ttttgggaat tgtttgtagt aaatggatag
     2281 ctgtagaaat tcattgggca tttttgttag tattactttc cttttatttt ttgtctaatt
     2341 atttttatag aaaatacaat aaaagttggc tatatatttt aagttttatt agtctaataa
     2401 tttttggatt ggcttatact caaagatttc aattagcatt tcaaaagcat catttttcaa
     2461 aatttgaagg agattatgca attggcattg ttcgtgaacc tattgaaaac aagactaatt
     2521 cttatcaaac taccatagaa attaaaaata ttattcaaga gcataaaatt ttccatacca
     2581 aaggaaaatt attgttatat attcaaaaag atagcctcaa aaatccttta gaaattggag
     2641 atgaaatctt attgcactta aaaagtaatg aaatagaagc tccaaaaaat ttgaaacaat
     2701 ttgactacaa aacctactta tcttataatg atatttacca tcaagcatat attaaaaatt
     2761 ttgaatacaa aatattggct aaacatcaaa ggtattttat caaaagaaag agtgacgaaa
     2821 tttctaaata tgcacaaagt ttgctacaaa aatacattcc aaacaaggag aattttaata
     2881 ttgcagatgg cattttctta ggtcataaat cagaaatgga tattgaacaa tataacacgt
     2941 ttgcctatct aggcattgta catattcttt ctgtctctgg gctacacgtt ggaattatat
     3001 atttgctcat aagctttttt ttaagtttca ttcctaataa aaataaaact atttatatcc
     3061 ttaaattttt aactgcattt ttattgattt ggctttttgc atttgtagtc ggcttatctc
     3121 ccgctgttgt acgctctgct ttattgttta gtattctaca ttttggaaga atattgaaag
     3181 aagatacaat accattaaac attttattag gtgctgcatt tgtacaatta ttgattaacc
     3241 cactggtact ttacaatata ggttttcaat tatcgtattt agcaatgttg ggtttgtatt
     3301 tgttttataa acctatctat aacttactat attcagaaaa taaattcatc aattggttcg
     3361 cacaattatg ttcagcgagt attgctgcac aaatatttac actgccattt gccatctatt
     3421 attttggtaa ttttccgact tattttctta tagcaaatat tttcgccata ccactttcaa
     3481 cattaatttt atggttttgt gtcgcacttc ttccactaca atttattcct ttattggcaa
     3541 aattaacagg acaactaatt tcttatttaa tagatgtctt tatctatctt tcaaagcttt
     3601 tgttattgtt accttatgca aaaattagta ctttatttat aaatgaatgg caattaattt
     3661 tatgctgttt agcaattgct atttttacaa tttatatatt ctctcaaaaa attaaatatg
     3721 ctttgtatgc attaagtatt gttgtgctta ttatcatttc tgcttattat tttcaatacc
     3781 aaaactacaa acaagataaa ttggttttct atgctgtcaa taagaactta gtttatgggt
     3841 ttcatcaaaa atcgaatgta actatatatt ctaaagacac tttaagccta aaggatttta
     3901 attttaatgt cagtaataca tgtagatatt ttagaaatca aacgataaac aacaatttaa
     3961 ttgttcaaga tttacaactt ataaaaagca aatctattta ttttttgcat cagaataata
     4021 ttcaaaaaag ttatcaagca ccattatcta ttgatatttt agtattgaca gataatatct
     4081 atttagatat agaaaaatta gtacaaaatt atcgcttcaa acaacttata ctttcttcag
     4141 atttagatta taaacatcag aaaatctata ccaagttatt gaatgaagca ggtatttctt
     4201 ttactgattt aaagcaacaa gcatacattg attataacat ataaaatgaa atttattttt
     4261 atatacattc aaagattaat atcttcgtct aaaattattc tataatggaa tttatcaaaa
     4321 ttaataagaa agaaaattat gctatcctcc aaatggatag aggcaaatcc aatcccataa
     4381 accagcaatt tgtcgatgaa tttattgagg ctttaaatca attaatggca gataatgaag
     4441 ttttgggtgt cgttgttgct ggaaaagaaa atttcttttc ggcaggttta gatattccag
     4501 aattatatag ttatgatgaa gcacaattcg atgtattttg gaaaaaattc ttacaaatga
     4561 ttttgcaact atcgaaattt aataaaccaa tggttgcggc tattactgga catgcacctg
     4621 ctggtggttg tatcattgcc aatggttgtg attatagagt tatggcagat ggaaactata
     4681 agattggatt gaatgagatt ccagttggaa ttgtggttcc aaaaggtgtg tatgcacaat
     4741 acagcatgtg gattggcaca cgtaatgctt atcagtattt aatggaaggt aaattatacg
     4801 cacccaaaca cgctcaagaa attggtttaa tagatgaagt agttccttta gaaaatgtat
     4861 tagaaagagc agaaacaaaa ttgaaacaat atttacaata tgaacaatct ggttggagat
     4921 tgacaaaaac gcaacttaga caatctgttg tagatacaat ggaaagctta acagataagg
     4981 aaatggtaat gtttcaaaaa caatggtgga gcgaatctgt acgtgctatt ttaggtgcgt
     5041 ttattgacaa attaaagaag taattataat acagctgcaa ttttttgtaa tttagaaagt
     5101 aaattttcta ctttattaag agctatcata ttggaaccat cacttaatgc attacttgga
     5161 ttagggtgcg tttctaagaa taagccatca gctccagcaa caatgccaca ttttgctata
     5221 agctcaataa attcaggtgt gccgagtgtt ttaccttcag tcgaattggg ttgttgaata
     5281 ctatgtgtta tgtcaataat tgctttacaa tgattagttt ttaaaagagg aatattccta
     5341 aaatcaacca ctaaatcttg atatccaaat gaatttccac gttcagtcag ccaaatatta
     5401 tcattaccta cactgcgaac tttatctgcc gcaaattgca tttggattgc atttgcaaat
     5461 tgtccttttt ttatattgat gattttttca gttttagcag ctgctaatat taaatccgtt
     5521 tgccgacata agaaggctgg tatttgtatt acatcaacaa tatcttttac tttttcaata
     5581 tcagaagatt catgcacatc ggtcaatatt tgaacttgat actttgattt aatatctgct
     5641 aaaatctgta atgctttagt ttctcctatg gttgaaaacg aatgaataga ggttctattg
     5701 gcttttttaa aagaagcttt aaaataaaaa ggaatattta atttttgagt aatagaaacc
     5761 aatgtttctg caacttcaaa accgatttct tgattttcaa ccacacaagg tccagctata
     5821 agaaaaaaat tactcaactt aaaaattgtt ataaaaacaa acttagtata attttagaaa
     5881 ataagaaaga aatgaaatag aattacttat aataaaccta aatcactcaa ttttgattta
     5941 attctattgc ttaaatcttc atttactggc atcaatggta atctaacagt ttcgctacaa
     6001 attcctaaat gatgtaatgc acattttaca ccaccaggat tgccttgctc aaataacata
     6061 ttcactaaat catctaaagc aaaaactgtt tgtctcgcat cagcataatt accatcaagc
     6121 gcttgatgta ccatttcaga aaatattttt ggagtagcat tagcaatcac agagataacg
     6181 ccatcataac ctaaagaaat ctgaggaata ataatatcat catttccaga aattaaataa
     6241 aatccatcag gtttatggtg tgccaattct ataatttgag accaatcaga agttgcctct
     6301 tttatggcaa taatattttt aaaattttct gccaatttaa gcgtcgtaga agccttaata
     6361 ttgctcgatg ttcttccggg aacattgtat aaaatcacag gcaattctgt acttaatgca
     6421 attgttttat aatgttcata cagaccatct tgagttggtt tattgtaata cggagaaact
     6481 gaaagaattg catcaacacc tgataagtca aacttattca aatcagcaac aattttttgg
     6541 gtatagttcc ctccaaatcc agcaattact ggcacttttc cagctacgtt attgactgtt
     6601 gctctaatca tgtcttgtct ctcctcatcg gacatagtag ccacttctcc tgttgtacct
     6661 aatacaacaa cataatcgac atgatttgca ataacatgct tacaaagctg ttcatattga
     6721 ttaaaatcta tctcaccttg ttcattaaat ggagtaacta aagctacacc aacaccacga
     6781 agattaatac tactcataaa ttgttttaaa atgaagccct aatttctaaa atttatttga
     6841 attatctaaa tttaattcat taaatccata ataaatattt ttttttaaat gtattgattc
     6901 gtttaataaa tgcttgttca ggatatttta tcaattgata ttgttgaagt ttgatagttt
     6961 ttaagtaata taaaacgtag tattgagaaa aaatatcaaa aaaaataata ctgaatgttg
     7021 ttttataaga aaaagatatt atctttgcat tcgcttttgg agttattgtt tcaatagttt
     7081 cagaaacgga aggttcggta gttcagttgg ttagaataca tgcctgtcac gcatggggtc
     7141 gcgggttcga gtcccgtccg gaccgcaaaa aaagtcctca aatttttgag gacttttttt
     7201 attt
//
LOCUS       DAOQCW010000121         6854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_4489_length_6854_cov_4.922783, whole genome shotgun sequence.
ACCESSION   DAOQCW010000121 DAOQCW010000000
VERSION     DAOQCW010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6854
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4489_length_6854_cov_4.922783"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..896
                     /locus_tag="PLS16_09790"
     CDS             195..896
                     /locus_tag="PLS16_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNJ61414.1"
                     /translation="MKLLAKTYFSDIYELFYPKICGACNGMLLKGEMHLCFTCLNNLP
                     FTKFENIKENPAQKLFEGRIPIEFATSYLYFSKSELTQNILHNIKYRYRKQLATFVGQ
                     QLGASILQKHQEHQFDSIIPVPLHPKKTALRGYNQSELIAEGISVVLNVPINTKTIRR
                     IKNTETQTQKNKTERWENIKDAFELIDEKAIYNKHILLVDDVLTSGATLEACASKLKI
                     NDNIKISIATLAIAF"
     gene            955..2361
                     /locus_tag="PLS16_09795"
     CDS             955..2361
                     /locus_tag="PLS16_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61415.1"
                     /translation="MLIISCKKDKNFSVNTLSFSNDTLKFDTVFTTLGSTTKFMTIKN
                     NEHKAIKIEELKLSNPSNYRINVDGDTGTIFRDIIIPANDSIYVFVEVTINPNSANLP
                     FVLLDSIQLKMENKHQFVYLQAYGQNAHFFDGDTIQTNTTWTNDLPYVILNYLSVDKN
                     STLNIQSGCKVYFGGAAAMLVEGKLNIQGGNDTSEAVLFKTYRLDKDISGTAYEKYPG
                     QWAGLFFLRGSEGNIEYLNLQNAQYGINVGNILITDDVVENTQKLNDAKLSNGAKLTI
                     KNSRIYNNSFYNIFGFLSTIQAENLLCYGAGTNIIGLYHGGDYSFINCTFFAQSNAYI
                     SHSKTPAFYFSNYFMVGNTPIYADSSNLLVRNSIIYGTLNEELFLDDISENTHKLYYT
                     FNHSCIKNQTGNLMSNMYFQNCINTDPKFENILKSDFRLQDASPCINGGNDIDFYPLI
                     DLSGKLRIGKPDIGALEK"
     gene            2569..>3886
                     /locus_tag="PLS16_09800"
     CDS             2569..>3886
                     /locus_tag="PLS16_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61416.1"
                     /translation="MSFFVVGIAMGGFMMTWNVCVYILDVSKFDFIVYVSKPFTRFVL
                     NNTILPLIFTIAYIIKIFLFQSHQGLQGPFHILLHIVGLILGNISMIILYSIYFIYFA
                     DDIKTFIEKLSNKSKENLERKNIKLDIFDIRKGSSSNKKWKVEHYWYYPWRVYKVSDI
                     PIYDQKLIDAVYYLHHRNIFAIIVISLTSLIALGFLMDYLDFSIPAGATLFLLLTVFS
                     VVFTLFLFWFKSWITIAFLGVIFILNILTRYDLVVYKNKIFGLKYDSKVSYNYNVIEA
                     QSNDSNFEDDKIYTEQILNNWKNKNQNKRKPKLVILNVGGGGSKAAYWTFHVIQKLNQ
                     ATKQKLFKRTVLISGASGGMYGSAYYRELYLQAQKDTSIHLDNAKYLDNIGKDLLNAV
                     MTSASTNDIFYPFRKVNYGSIRYNKDRAYYFDREFNKNTNFILDKKL"
     assembly_gap    3887..3986
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3987..4772
                     /locus_tag="PLS16_09805"
     CDS             <3987..4772
                     /locus_tag="PLS16_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61417.1"
                     /translation="YFDREFNKNTNFILDKKLMDYKLPESNADIPMMVIGSTIIEDQR
                     MMYFSPQPISYLLKPYIHSDKINKQYTITDAIEFMRYFKNNRAEDVGLVCALRTGATY
                     PYILPSVSMPTEPRIYAMDAGFRDNYGYIVTARYMNAFKDWIEANTSGVIVVTIKSNE
                     RKEHNKDEKHTYIGELLSPIGGIYSNMFNLQNYNADQSFAIIFEDYKTKINFIDFIYI
                     PSEENNKASLSWHLTEKEKRDILNSYNNATNQQMEQKLLELLY"
     gene            4829..5392
                     /locus_tag="PLS16_09810"
     CDS             4829..5392
                     /locus_tag="PLS16_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61418.1"
                     /translation="MKKNIFIVLLFVVIFTQEVKAVEKGDFHMNITTNLGHHAYIGSG
                     LNGNFKKAGVGFIPGVTMNMDYAVSPYFSFGGWFTFSGKKYAEDLLKYRYFGVGVRGN
                     FHIYQLISEKGNAKLDADKFDLYIPLHIGGGFKLKDKVSGSPFGGGVIVGSGVGATYY
                     FVDNIGVNLETGYNEASYAKIGLSFKF"
     gene            5629..>6854
                     /locus_tag="PLS16_09815"
     CDS             5629..>6854
                     /locus_tag="PLS16_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61419.1"
                     /translation="MKIPKNLRFSISENYNIINSQRTFFLTFLLLLTVDYINAASSVK
                     FPSKTQPDIAILSKQEGKWIFSNQLFTASFIQHGDKLFFGGSTEMCIEPGTELFELTL
                     GNNTKVKASEMKWQSIYEENLSANQTVLKASEQLPGKALVAILTYGELTIQWRAVLRD
                     SSHYLRTEMKISTSKNIQMKSITPMLYKIKDYFGNTKPLVIGNTRGAVIASNHIFAGL
                     ETPLGINSVKSTFNSNFDIYSWNKNSWQETVEAIPDSILKFGFKSSQILVSKGEVNIK
                     ASGILQFKFAYKSGQHRMNITGIDLVDETGNIVASDYHLGYAGKKSLQNIYSLNIKNS
                     GRYTLRYFAENQTETINSKGKILIISDSDNNNNEIAIIPKEKSDSYTIISGKWNRNTI
                     LNTTDTFYVSSVIGMVA"
BASE COUNT         2424 a          869 c         1093 g         2368 t
ORIGIN      
        1 tgatgattac gatgataggt atgatgaaca acattatatt tttattgcaa aagatggtga
       61 atttatagca atagattaag aaactctctg agtaattagt gttagtaatg gccgcagaaa
      121 attgaattca attttctgcg gtttttcttt tttatgaaat gaattttttt gtacttttag
      181 tttaagctaa gaaaatgaaa ttactcgcta aaacttattt ttctgatatc tacgaattgt
      241 tctatccaaa aatttgtggt gcttgtaatg gtatgttatt gaaaggtgag atgcatttat
      301 gttttacttg cctcaataat ctacctttta ctaaatttga aaatataaaa gagaatccag
      361 ctcaaaaact atttgagggt cgtattccaa ttgaatttgc aacttcctac ctttatttct
      421 ctaaatcaga attgactcaa aatattttgc ataatataaa atatcgctat cggaaacaat
      481 tggcaacttt tgttgggcaa caacttggag catcgatttt gcaaaaacac caagaacatc
      541 aatttgatag tataattcca gttccattac atccaaaaaa gacagctttg cgaggttata
      601 accaaagtga attaattgca gaaggcatat ccgttgttct aaatgttcca ataaatacaa
      661 aaaccattcg aaggattaaa aatactgaaa cacagacaca aaagaacaaa acagaaagat
      721 gggaaaatat taaagatgca tttgaattga tagatgaaaa agcaatttac aataaacata
      781 ttttattagt tgatgacgtt cttacttcag gtgcaacatt agaagcctgt gcaagcaaat
      841 tgaaaataaa tgacaatata aaaattagta tagcaacttt agcaattgct ttttagttat
      901 atttacaaag agatgaaatg cctacccaca aatattattc ttatcctact aataatgctt
      961 atcatttctt gtaagaagga taaaaatttt tctgtcaata ccttgagttt ttctaatgat
     1021 acactaaaat ttgatacagt ttttacaact ttaggttcga ctacaaaatt catgacaatt
     1081 aaaaataatg agcataaagc aataaaaatt gaagagttga aattgtccaa tccttcaaat
     1141 tatagaataa atgtagatgg cgatactgga acaattttta gagatattat tattcctgca
     1201 aatgatagta tttatgtttt cgttgaagta actataaatc caaatagtgc gaatttgcct
     1261 tttgttttgt tagattctat tcaattaaaa atggaaaaca aacaccaatt tgtttactta
     1321 caagcttatg gtcaaaatgc tcattttttt gatggagata ccatccaaac aaataccact
     1381 tggacaaatg atttacctta tgttatacta aattatttgt cagtagataa aaatagtacg
     1441 ctaaatattc aatcaggctg taaagtatat tttggtggtg ctgctgccat gttggtcgaa
     1501 gggaaattaa atattcaagg tggaaatgat acttctgagg cggtattgtt taaaacctat
     1561 cgtttggaca aagatatttc tggtacagct tatgaaaaat atccagggca atgggcagga
     1621 ttgttctttc taagaggtag cgaagggaat attgagtatt tgaatctaca aaatgctcaa
     1681 tatggtatca atgtcggaaa tattttgatt acagatgatg tagtagaaaa tacacaaaaa
     1741 ttgaatgatg ctaaattgtc caatggagca aagctaacca taaaaaactc aagaatttat
     1801 aataattcat tctataatat ttttgggttt ttaagtacaa tccaagcaga aaatctattg
     1861 tgttatggtg caggtacaaa tattattggc ttgtatcatg gaggtgatta tagtttcata
     1921 aattgtacat tctttgcaca aagcaatgca tatatttcac acagcaaaac accagctttc
     1981 tattttagta actattttat ggtcgggaat accccaattt atgctgattc ttccaattta
     2041 ttagttagaa attctattat ttatggcact ttaaatgagg aattgttttt agatgatatt
     2101 agtgagaata cacataagtt atattacaca tttaaccact cttgtatcaa aaatcaaact
     2161 ggcaatctta tgtcaaatat gtattttcaa aattgtatta ataccgatcc aaaatttgaa
     2221 aatatactta aatcagattt taggcttcaa gatgcttcgc cctgcataaa tggaggtaat
     2281 gatatagatt tttatccttt aattgattta tctggaaagt tgcgaattgg caagcctgac
     2341 attggtgcat tagaaaaata aagtaatatg tggttacata ttaagcaaat aggctattgg
     2401 gtttttaatt ctttccctgt aaagttgttg ctacttcatt taaaaaaaca cctttatctc
     2461 aatttagttt ggttattatt attccttatt ttatttgaaa gtttaggaaa acagtacggc
     2521 gttcctttat tacttcttga ccctgaattt tttggaaaag tcaatttttt gagttttttt
     2581 gtagtaggca ttgcaatggg aggattcatg atgacttgga atgtatgtgt ttatattctt
     2641 gatgttagta aatttgattt tatagtttat gtttccaaac catttacaag atttgtttta
     2701 aataatacaa tcttaccttt aatatttaca attgcctata tcataaaaat atttctattt
     2761 caaagccatc aaggactaca aggtcctttt catattttat tacatattgt tggcttgatt
     2821 ttaggaaata taagtatgat tatattgtac tcaatttact ttatttattt tgctgatgat
     2881 attaaaacct ttattgaaaa attatcaaac aaatcaaaag aaaacctaga gcgtaaaaat
     2941 ataaagttgg atatttttga tattagaaaa ggtagttcta gtaacaaaaa atggaaagtg
     3001 gaacattatt ggtattatcc ttggcgagtt tataaagtaa gtgatattcc aatttatgat
     3061 caaaaattaa tagatgcagt ctattatctc catcatagaa atatatttgc gattattgtt
     3121 atcagtttaa cttctttaat tgcattgggt tttctaatgg attatttaga ttttagtatt
     3181 ccagcaggag caacactttt cctcttattg acagtttttt cagtggtatt tactttgttt
     3241 ttattttggt tcaaaagttg gattactata gcatttttgg gtgtgatatt tattttaaat
     3301 attctaactc gttacgattt agtggtatat aaaaataaaa tatttggttt aaaatatgat
     3361 agtaaagtat cctataatta taatgtaatt gaagcacaat ctaatgattc taactttgaa
     3421 gatgataaga tatatactga acaaatatta aataattgga agaataaaaa tcaaaacaaa
     3481 agaaagccca aactggttat actcaatgtc gggggtggtg gttctaaagc tgcttattgg
     3541 acattccatg taattcaaaa attgaatcaa gctaccaaac aaaaattatt taaaagaact
     3601 gtcctaatta gtggagcatc tggtggaatg tatggttcag catattatag ggaattatat
     3661 ttacaagctc aaaaagatac aagtatccac ttagataatg caaaatactt agataatatt
     3721 gggaaagatt tattgaatgc agttatgaca tcggcttcaa caaatgatat tttttatcct
     3781 tttagaaagg tgaattatgg ctcgattaga tataataagg acagagctta ctattttgat
     3841 cgagaattta ataagaatac caattttatt ttggataaaa aattgannnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnntatt ttgatcgaga atttaataag aataccaatt
     4021 ttattttgga taaaaaattg atggattata aactacctga atctaatgct gatattccaa
     4081 tgatggttat aggttctact ataattgaag atcaaagaat gatgtatttc tcgccgcaac
     4141 caattagcta tttgctcaag ccttatatcc attcagataa aattaataag caatatacaa
     4201 ttacagatgc cattgagttt atgcgatatt ttaaaaataa tagagcagaa gatgttggat
     4261 tggtttgtgc actccgtaca ggagccactt atccatatat tttgccttct gtaagtatgc
     4321 caactgaacc aagaatttat gctatggatg caggatttcg tgataattat ggatatatag
     4381 ttacagcaag gtatatgaat gcatttaaag attggataga agcaaatact tcaggcgtta
     4441 ttgttgttac gataaaatca aatgaacgaa aagaacataa taaagatgaa aagcatactt
     4501 atataggaga attgttatcg ccgattggtg gtatttattc caatatgttt aatcttcaaa
     4561 actataatgc tgaccaatcc tttgcaatta tttttgagga ttataaaacc aaaattaatt
     4621 ttattgactt tatttatatt ccatcagagg aaaataataa agcatctttg agctggcacc
     4681 taactgaaaa ggaaaaaaga gatattttaa attcatacaa caatgctaca aaccaacaaa
     4741 tggaacagaa actattggaa ttattgtatt aatgataaaa tattgtagta atcgttgttt
     4801 ttatatattt ttgcaaaaaa gatttaatat gaaaaaaaat atttttattg ttttgttgtt
     4861 tgttgtaatt tttactcagg aagttaaggc agttgaaaaa ggagattttc acatgaatat
     4921 aacaaccaat ttaggacatc atgcctatat tggctcaggt ttgaatggta attttaaaaa
     4981 agctggtgtt ggttttatac ctggcgttac gatgaatatg gactatgcag taagccctta
     5041 ttttagtttt ggtggttggt ttactttctc agggaaaaaa tatgctgaag atttattaaa
     5101 atatagatat tttggagtcg gagtacgtgg aaatttccat atttatcaat tgatttctga
     5161 gaaagggaat gcaaagttag atgctgataa gtttgattta tacattccat tacatattgg
     5221 cggtggcttt aaattaaaag acaaagtgag tggaagtcca tttggaggag gcgtaattgt
     5281 tggttcaggt gttggtgcta cttattattt tgtagataat atcggagtaa atttagaaac
     5341 tggttataat gaagcttctt atgcaaaaat aggtttgtct tttaaattct aatttagttt
     5401 ttgtttttat taaaaaagta gaatgaggct gtctgaaaat ttaggacagc ctcttttata
     5461 tttataggtt atctacacca cgaattgaag aaagtaaaac caatacgctt gaaattaaaa
     5521 cagacgctct gaataacaat tacatcatta gttttataaa taaaaagcat actatattca
     5581 tacaaataat ttattgtaaa agtgattttt tatctaactt tataacccat gaaaatacct
     5641 aaaaaccttc gtttttctat atcagagaat tataatataa taaatagtca gagaacattc
     5701 tttttaacat ttttgttgtt gcttacagta gattatataa atgctgcgag ttcagtaaag
     5761 tttccttcta aaacgcagcc agatattgca atattatcga agcaagaggg gaaatggata
     5821 ttttctaatc aactttttac agcatctttt attcaacatg gagacaaact cttttttggt
     5881 ggctctactg aaatgtgcat tgagccagga acagaacttt ttgaattgac gcttggtaat
     5941 aatactaaag tcaaagcctc ggaaatgaaa tggcaatcta tatatgaaga aaatcttagt
     6001 gctaaccaaa ctgttcttaa agcttccgaa caacttcctg gaaaggcttt agtagcaatt
     6061 cttacctatg gcgaacttac tattcaatgg cgtgcagtat tgagagatag ttctcattac
     6121 ttacgaacgg agatgaaaat ctccacatct aaaaatatac aaatgaaaag tatcacccca
     6181 atgctttata agataaaaga ttattttggt aatacaaagc ctttagttat tgggaataca
     6241 cgtggtgccg ttatagcttc caatcatatt tttgctggat tggaaactcc tctaggaatt
     6301 aattctgtga aaagtacttt taattcaaat tttgatatat attcttggaa taaaaattca
     6361 tggcaagaaa cagtagaagc cattcctgat tctattctta aatttggctt taaatcttcg
     6421 caaatattag tatctaaagg agaagtaaat atcaaagcat ctggaatatt gcaatttaaa
     6481 tttgcatata aatctggaca acatcgaatg aatattacag gcattgatct agttgatgaa
     6541 acaggaaata ttgtggcttc tgattatcat ttaggatatg ctggaaaaaa gtcattacaa
     6601 aacatctatt ctctaaacat taaaaattct ggtcgttaca cgttacgata ttttgcagaa
     6661 aaccaaacag aaacaattaa tagcaagggt aaaattttaa ttattagtga tagcgataat
     6721 aataataatg aaattgctat aatacctaag gaaaaatcgg attcatatac tataatttca
     6781 ggtaaatgga atagaaatac tatcttaaat acaacagaca ccttttatgt ttctagtgtt
     6841 ataggaatgg ttgc
//
LOCUS       DAOQCW010000122         6644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_4674_length_6644_cov_7.033996, whole genome shotgun sequence.
ACCESSION   DAOQCW010000122 DAOQCW010000000
VERSION     DAOQCW010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4674_length_6644_cov_7.033996"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="PLS16_09820"
     CDS             complement(<1..1109)
                     /locus_tag="PLS16_09820"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNJ61420.1"
                     /translation="MKKVVVSGAGGHIGFNVAKKLLEKQYEVLLLVRSINVNIIELKN
                     LGAKIHSCNLLDGKTYLPQIENFDAFFHLAAENTTSMQNEARVLENTLQLTKCVAEAC
                     VKAGIQTLIYTSSVVVLGRSKDTNRLINENDVVEKPESPYVKGKLEAEKFLIQFAKDK
                     NLDIRHIYPSWVVGPGDAKLTPPHKVIKDFVSKGQAFYFDGGISIADVEAVADAQISA
                     LENGTPNGKYIIGGENITFNTFYEILSGFSGKKQPKLKLPKWFIYFGALVLDLVLKVF
                     GKRSPVSPEYVESVVGNYSWYDSNKAIKELGYQPEAAKEILRKAIQETYKRMAGTINL
                     GKKTNLLPEITTPSQEKTLMITGVPGWLGNRMVDI"
     gene            complement(1159..2100)
                     /locus_tag="PLS16_09825"
     CDS             complement(1159..2100)
                     /locus_tag="PLS16_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ61421.1"
                     /translation="MKKILITGVAGFLGSHLADRFIKEGYHVIGMDNLSTGSIQNIEH
                     LFPNPNFEYYHCDVTKFVHVPGKLDYILHFASPASPIDYLKIPIQTLKVGAIGTYNLL
                     GLAKEKNARILVASTSEVYGDPEVHPQPEEYWGNVNPIGPRGVYDEAKRYMEAITMAY
                     HTFHQVETRIIRIFNTYGSRMRLDDGRALPQFMSQALRGEALTVFGDGSQTRSFCYVD
                     DLVEGIYRLLLSDYHLPVNIGNPDEISLLDFAKEIQKLIGTDVEIVFRDLPKDDPKQR
                     QPDITKAKNILGWEPKVGRAEGLKITLEYFKDKVLNS"
     gene            complement(2097..3434)
                     /locus_tag="PLS16_09830"
     CDS             complement(2097..3434)
                     /locus_tag="PLS16_09830"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNJ61422.1"
                     /translation="MKIAVIGTGYVGLVSGTCLAETGNYVTCVDIDINKVQKLQSGIV
                     TIYEPGLENIFQRNIKEGRLTFTTQIEEAIKDATLIFLALPTPPNEDGSADLSYVLGV
                     ADQLGKIITDYKVIINKSTVPVGTADRVKAAVAKNATCDFDVISNPEFLREGVAVDDF
                     LKPDRVVVGTSSARAKKLMEELYKPYVRQGNPIIFMDERSAELTKYAANSYLATRISF
                     MNELALLAEKLGANIDNVRIGMGSDNRIGRRFLFPGIGYGGSCFPKDVQALAKTAKEN
                     EYDFKILNAVMNVNHIQKHVLSEKILNYFGTDLSGITIGIWGLAFKPNTDDIREAPAL
                     YIIQELLDAGAKILAYDPEASANVQQYFSKLDENQKSRLIFVQNSWEAIENADVLAIL
                     TEWAEFRGADLKEIKAKLKQPIIFDGRNIYDLEAVEEVGFYYNSIGRKTINKI"
     gene            3566..4561
                     /locus_tag="PLS16_09835"
     CDS             3566..4561
                     /locus_tag="PLS16_09835"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNJ61423.1"
                     /translation="MKVALCIPKYYDTSVKIYIDNLIPFIEKHQVEIVKVQHNEPIPN
                     DIDLVWEPNCTGAKYPNKRIIFSNKKWVVTLHGTSNLSLPLHYTFSTFKEKMIGWLKN
                     KRRQFFWFFYKYKVAHIITVSKFAKEEIVEYLHIPPTKISVVYHGFNDTLFFPRSGEK
                     TYFLHVSMYQKVKNLDRLIEAYEQIPLNKRIPFIIIAPNYPNIDIQIEKLTLITEKLP
                     QKEIVKYMQSAYCFIFPSIRESFGLPILEAFGCGIPVISSNTSALKEIVGDAGILVNP
                     FSVEEMNKAMLNIISDNDLRNELALKADNRAKDFSWEKSAYLHFEIFNRICLSKQ"
     gene            4564..5553
                     /locus_tag="PLS16_09840"
     CDS             4564..5553
                     /locus_tag="PLS16_09840"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNJ61424.1"
                     /translation="MNITYKYNPNLKFIKEGIKGNLFYDNQFVFDKEIERVPYERFWK
                     WMLGKNPQGREKKADKWCPQVIKNNSFVEDKQDKIVWLGHSSFYMQLNGIRILTDPIF
                     YHVLPVRIRRRHKLPCSVATLKNINLILLSHGHRDHLDVPSLKQILKYNPDVEILCPL
                     GFKKMLQKIGFKKIQEAAWWQQYNFTHLQIDFLPAKHWNRRHLLDFNKTLWGSFAIQY
                     QNKKIYFAGDTAYAPHFKEIQQYYNYFDIALMPIGAYKPKFIMDWAHISPKEAMLASN
                     EMNVKNFIPMHYGTFDLSDEPASEPIQKISKYKKEERLDANLHCLDVGKVYLV"
     gene            5673..6476
                     /locus_tag="PLS16_09845"
     CDS             5673..6476
                     /locus_tag="PLS16_09845"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61425.1"
                     /translation="MRYILLSLLVGITLFSKGGELNKVRKAADQGESNAQYKLGTMYY
                     MGNEVKEDKQLALYWIKKSAEQGNADAQYALGLMYMAGVAVEKDKNQAIFWIEKSAKQ
                     GNSNAQFDLAKLYDNGNTTKSEKSEVKNTKELYAKSAEQGNTEAQIKLGEMYFDGDGV
                     EINKIEAFKWFKKAAEQGDINAQYTIGEMLYNGDGVLLDKKAAIYWYTKSANQGNNEA
                     QYNLGYMFFKGDGMPVNKKKAAYWIRKAFDNGNPRAKDLWEDLELWQYE"
BASE COUNT         2315 a         1105 c          963 g         2261 t
ORIGIN      
        1 aaaatatcca ccattctatt gcccaaccaa cctggaacac cagtaatcat caatgttttt
       61 tcttgactag gtgttgtaat ttctggcaat aaattggttt tcttccctaa attaattgtt
      121 cctgccattc gtttatacgt ttcttgaata gcttttctca atatttcttt tgcggcttct
      181 ggttgataac ctaattcttt aatggcttta ttgctatcat accaagaata attcccaaca
      241 acagactcaa catattcagg actgacggga gaacgctttc caaatacttt taaaaccaaa
      301 tctaaaacta acgctccaaa atagataaac cattttggca attttaattt aggttgtttc
      361 tttccactaa aacctgataa tatttcataa aaagtattaa aagtaatgtt ctctccacca
      421 ataatatatt ttccatttgg tgttccattt tctaaagcag aaatttgggc atcagcaacg
      481 gcttccacat cagcaataga aattccacca tcaaaataaa atgcttgtcc tttgcttaca
      541 aaatctttaa taactttatg aggtggcgtt aatttggcat ctcctggtcc aactacccaa
      601 gaagggtaaa tatgacgaat gtctaagttt ttatctttag caaattgaat gagaaatttt
      661 tcagcttcta atttcccttt tacataagga ctttcaggct tttctactac atcattttca
      721 tttattaagc gattggtatc tttacttcga cctaatacaa ctacagaact tgtataaatt
      781 agtgtttgaa ttccagcttt tacacaagct tctgcgacac attttgtaag ttgcaaagta
      841 ttctctaaaa ctcgagcttc attttgcata gaagttgtat tctcagcagc taaatgaaaa
      901 aaggcatcaa agttttctat ttgtggcaaa tatgttttac cgtctaataa gttacaggaa
      961 tggatttttg cacctaaatt ttttagttca ataatattga cattgataga acgaactaag
     1021 agaagaacct catattgctt ttctaataat ttttttgcca cattgaaccc aatatgacca
     1081 cctgcacctg aaacaacaac ttttttcatt aatcctttaa tggcatcaaa agtataaaaa
     1141 aataagcaat tttaattctt aggaattaag aactttgtct ttaaagtatt ctaaagttat
     1201 ttttaaacct tcagcacgtc caacttttgg ttcccaacct aaaatatttt ttgccttagt
     1261 aatatctggt tgtctttgtt tggggtcgtc ttttggtaaa tctctaaaaa ctatttcaac
     1321 atctgtacct atcaattttt gtatctcttt agcaaaatct aatagtgaaa tttcatcagg
     1381 gtttccaata tttactggca aatgataatc agataataat aatctgtaaa tgccttcaac
     1441 taaatcatct acataacaga atgagcgagt ttgtgagcca tctccaaata cagttaatgc
     1501 ttctccacgt aaagcttgag acataaattg aggcaaagct cgtccgtcat ctaaacgcat
     1561 tcttgaacca tatgtattga aaattctaat aattctagtt tctacttgat gaaaagtatg
     1621 ataagccatc gtaatcgctt ccatatatct ttttgcttca tcatatacac ctcgaggtcc
     1681 aataggattt acattccccc aatattcttc aggctgagga tgtacttcag gatctccata
     1741 aacttcagag gttgatgcaa ctaatattct tgcatttttt tcttttgcta aacctaataa
     1801 attataagtt ccaatagctc ccacttttaa agtttgaatt ggaattttta aataatcaat
     1861 aggcgatgct ggcgatgcaa agtgtaatat ataatctaat tttcctggta cgtgtacaaa
     1921 ttttgtaaca tcacaatgat aatattcaaa atttggatta ggaaatagat gttctatgtt
     1981 ttgaatactt cctgtactta aattatccat tcctattaca tgatagcctt cttttataaa
     2041 tctatctgcc aaatgagaac ctagaaatcc agcaacacct gtaattagta tttttttcat
     2101 attttattta tcgtttttct tccaatgcta ttgtaataaa atccaacttc ttcaacagct
     2161 tctaaatcat aaatatttct tccatcaaaa attataggtt gtttcaattt tgctttaatt
     2221 tctttcaaat cagctcctct aaattctgcc cattccgtta gaattgctaa aacatcagca
     2281 ttttctatgg cttcccaact attttgaaca aaaatcaatc tcgacttttg attttcatct
     2341 aatttactaa aatattgctg cacattggca cttgcttctg gatcgtaagc tagaatttta
     2401 gcaccagcat ccaataattc ttgaatgata tacaaagcag gagcttctct aatatcatca
     2461 gtatttggct tgaatgctaa accccatatt ccaatggtaa ttcccgacaa atctgttcca
     2521 aaatagttta atattttttc agataaaaca tgcttttgga tatgattaac attcattacg
     2581 gcatttaaga ttttgaagtc atattcattt tctttggctg ttttggctaa tgcttgtaca
     2641 tcttttggga aacaacttcc tccataccca ataccaggaa ataaaaatcg tcttccgatt
     2701 ctgttatcac ttcccattcc aattcttaca ttatcaatat ttgctcctaa tttctcagct
     2761 aataatgcca attcattcat aaaggaaatt cttgttgcca aataagaatt tgcagcgtat
     2821 tttgttaatt cagcactacg ttcatccata aaaataatag gattcccttg acgaacataa
     2881 ggcttataca attcttccat tagcttttta gcacgagctg atgatgtgcc aaccaccact
     2941 ctgtctggtt ttagaaaatc atcaacagca acaccttctc ttaaaaattc aggattagaa
     3001 attacatcaa aatcacaagt tgcatttttt gcaacagccg ctttaactct atctgcagtt
     3061 ccaacaggaa ccgtactttt attgatgata actttataat ctgtaatgat tttacctaat
     3121 tgatctgcaa ctccaagaac ataagacaaa tcagctgagc catcttcgtt gggtggcgtt
     3181 ggtaatgcca agaaaatcaa agtcgcatct tttattgctt cctcaatttg agtagtaaat
     3241 gttaatcttc cctctttaat atttctttga aatatatttt ccaaccctgg ttcatagatt
     3301 gttacaatac ctgattgtaa tttctgaact ttattaatgt caatatctac acacgttaca
     3361 taatttcctg tttcggctaa acacgttcct gacaccaaac ctacatatcc tgttccaata
     3421 actgcaatct tcatcctatt ttgagttttt tttacaaatc tataaaaaat aatagatttt
     3481 acgttttttt tacaaatcta ttttataaat cgatacaaaa tgaaatagat acaagagaaa
     3541 ttctttatgt atcttagcaa attctatgaa agtagcatta tgcattccta aatactatga
     3601 tacaagcgtc aaaatttaca ttgacaatct aattcctttt atagaaaaac atcaggtaga
     3661 aattgtaaaa gttcaacata atgagccaat tccaaatgat atagatttag tatgggagcc
     3721 aaattgtact ggtgcaaaat atcctaataa aagaattatt tttagtaata agaaatgggt
     3781 agttacgcta cacggaactt ctaatctaag tttacctcta cattatacat tttcaacttt
     3841 taaagaaaaa atgataggct ggctgaaaaa taaaagacga caattctttt ggtttttcta
     3901 caaatataaa gttgctcata ttattacggt ttctaaattt gccaaagaag agattgtaga
     3961 atacttacat attccaccta caaaaatatc tgtagtgtat catggcttta atgacacttt
     4021 atttttccca agaagtggtg aaaaaacata tttcttacat gtttcaatgt atcaaaaggt
     4081 aaaaaatttg gatagattga ttgaagctta tgaacaaatt cctctgaata aaagaatccc
     4141 atttataata atagcaccca attatcctaa catagatatt cagattgaaa aactcacttt
     4201 aattactgaa aaattacctc aaaaagaaat tgtaaagtac atgcaatctg cttattgttt
     4261 tatctttccg tctattcgtg aaagctttgg acttccaata ttagaagctt tcggttgtgg
     4321 tattcctgtt atcagttcta atacttcggc tttgaaagaa attgttggag atgctggaat
     4381 tttagtaaac cctttttcag ttgaagaaat gaataaagca atgctaaata ttatttctga
     4441 caatgattta aggaatgaat tagccttaaa agctgataat cgagccaaag atttttcatg
     4501 ggaaaaatct gcttatttac attttgaaat ttttaaccga atatgccttt ctaagcaata
     4561 ataatgaata taacatacaa atacaatcca aatttaaaat tcataaaaga aggaattaaa
     4621 ggcaatcttt tttatgataa tcaatttgtt tttgacaaag aaattgagcg tgttccgtat
     4681 gaacgatttt ggaaatggat gttaggtaaa aatccacaag ggcgagagaa aaaagcagac
     4741 aaatggtgtc cacaagtcat taaaaacaat agttttgttg aagacaaaca agataaaatt
     4801 gtttggttag ggcattcctc attctatatg caattaaatg gaattagaat tttaactgat
     4861 cccattttct atcatgtgtt gcccgtaaga ataagaagaa gacataaatt accttgttct
     4921 gttgctactt taaaaaatat aaacctcatc ttattatctc atggacatcg tgaccatttg
     4981 gatgttcctt ctttgaaaca aatattaaaa tacaatcctg atgttgaaat tttatgtcca
     5041 ttaggattta aaaaaatgtt gcaaaagatt ggctttaaaa aaatacagga agcagcttgg
     5101 tggcaacaat ataattttac tcatttacaa attgattttt tacctgcaaa acattggaat
     5161 agaagacact tattagattt taataaaaca ctatggggaa gttttgccat tcagtatcaa
     5221 aacaagaaaa tttattttgc tggagacact gcttacgctc cacattttaa agaaatacaa
     5281 caatattaca actatttcga tattgcttta atgccaattg gtgcttataa acctaaattc
     5341 attatggatt gggctcatat ctctcccaaa gaagcaatgt tggcaagcaa tgagatgaat
     5401 gtcaaaaact ttatccctat gcattatggc actttcgact tgagcgacga acctgcttct
     5461 gagcctattc aaaaaatatc aaaatataaa aaagaagaac gactagacgc aaatttacat
     5521 tgccttgacg tcggaaaagt atatttggta taaatattgt atccaataaa ttggttatat
     5581 tagaaataac aacttaaaaa taaataaaga atcagcagtt aataataaaa aataattatt
     5641 ttcaacaaca attttaaata ctcaaataga taatgagata cattttgctt agtttattgg
     5701 tcggaattac acttttctct aaaggaggag aattgaataa agttagaaaa gcagcagacc
     5761 aaggagaatc aaatgcacag tacaaacttg gtacgatgta ttatatgggc aatgaggtta
     5821 aagaagataa acaacttgct ctatattgga ttaaaaaaag tgccgaacaa ggcaatgctg
     5881 atgctcaata tgctttagga ttaatgtata tggctggagt tgctgttgaa aaagataaaa
     5941 atcaagcaat tttttggatt gaaaaaagtg cgaaacaagg caattctaat gcacaatttg
     6001 accttgcaaa attgtatgac aatggcaata ctactaaatc tgaaaaatct gaagtaaaaa
     6061 atacgaaaga attgtatgct aaaagtgctg aacaaggtaa cacagaagct caaataaaat
     6121 taggagaaat gtattttgat ggcgatggtg tcgaaattaa taagatagaa gcttttaaat
     6181 ggtttaaaaa agctgctgaa caaggcgaca ttaatgctca atatacaata ggagaaatgc
     6241 tttacaatgg tgatggcgtt ttattagata aaaaagcagc catttattgg tacacaaaaa
     6301 gtgccaatca aggcaataat gaggctcaat ataatttagg atatatgttt tttaaaggcg
     6361 acggaatgcc tgtaaataaa aagaaagctg cttactggat aagaaaagct tttgacaatg
     6421 gaaatcctag agcaaaggat ttgtgggaag atttggaatt gtggcaatat gaataagatt
     6481 ccaactttaa taatattcac atcggttaca tcattttagt aagctaattt tggtgtatta
     6541 tttcattatc tttgtcgcgt gtttatagaa ttaaccaaag ttgaaacaga aatattaaag
     6601 aaaattgcat ttgctgctga tagcttacaa tttaaagcat tcgc
//
LOCUS       DAOQCW010000123         6635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_4684_length_6635_cov_4.714590, whole genome shotgun sequence.
ACCESSION   DAOQCW010000123 DAOQCW010000000
VERSION     DAOQCW010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6635
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4684_length_6635_cov_4.714590"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="PLS16_09850"
     CDS             complement(<1..709)
                     /locus_tag="PLS16_09850"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNJ61426.1"
                     /translation="MAVLFLVEVAAGKVKKASYEAASYAAQVATQLGTEAVGLALGTS
                     DNAELAELGIYGAKKVVHANNAGFDSFDAGAWAKAVSDAAAANGAKVVIIPQTLTGKA
                     VAPRVAVRLNAGIISGAVSLPDADFVVKKTAFSGKAFAFMKITSDNKVIAFVPNSVGA
                     TKGSGSATVEASDIAPAASKVTVKDVKREGGNVAPLPEAELVVSAGRGMKGPENWGII
                     EDLANVLGATTACSRPVA"
     gene            complement(711..1454)
                     /locus_tag="PLS16_09855"
     CDS             complement(711..1454)
                     /locus_tag="PLS16_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNJ61427.1"
                     /translation="MKFLVCISLVPDTTTKISFVDNDTKFNTDKVQWILNPYDEWYAL
                     VRALELKEANGGTVTVVNVGGAANDATIRKALAIGADDAIRIDASGDLDSYAIASEIA
                     AVAKSGGYDLVLLGKETISDNGSNVGGMVAELLDLPFISYASKLDVAGSTATIERDIE
                     GGEEILSVDAPFVISCAKGMAEARIPNMKGIMAAKTKPLNVVTPQGTASLTATVKYEL
                     PPAKSACKMISPDNVGELVQLLHNEAKVI"
     gene            1525..1851
                     /locus_tag="PLS16_09860"
     CDS             1525..1851
                     /locus_tag="PLS16_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61428.1"
                     /translation="MHLIRIQQLEEIVRKKPQDLFAIFALAKEYEKIDKIKNAIELIE
                     NLLVIDLNYLGAYYFLGKLYEKNEQINKALNIYEAGILIAQKQKNLHTLSELRNAKLN
                     LELEDI"
     gene            2161..2664
                     /locus_tag="PLS16_09865"
     CDS             2161..2664
                     /locus_tag="PLS16_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61429.1"
                     /translation="MRKNVLFLSMVALAFAMILPSCKNEDSYACEQSQFVGTYNGSHN
                     LSINQPIELSIPINDKIVVAAIAGSTDSVTIQSQTLGITLRAQVKDCKISIPASVSEF
                     TYDLGGMIGRVKISGVNATLTVRKNTTKGQVESVISVQTGTANTLDNDLIDDLSIANT
                     ELPGIFK"
     gene            2776..5217
                     /locus_tag="PLS16_09870"
     CDS             2776..5217
                     /locus_tag="PLS16_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ61430.1"
                     /translation="MRILLVTLLFTMFSLMSMAQNGILKGKITDAKSGEELIGVNIGL
                     KGTTEGTTTDFEGNYELSLPSGVHLIVLSYIGYEEKSETVTIKENETITLDIKLGESR
                     SLLDEVVVTTSKFERRIGEETVSIDVIKPQMIEKQNLLDAGDAVKRSPGVTVVDGQPN
                     IRGGSGWSYGAGSRVLILVDDMPALQPEAGFVSWDQFPIENIGQIEVIKGAASALYGS
                     SAMNGIINIRTAYPTSKPYFKFSVFGGVIDNPNPNEKIGVVDSFGNFTGVFSETKVNK
                     KWWDLDSITFNGGAIANGAINVNDTTFVNNSKQRPHNYGFSWNYREKLLKNKKLDFIF
                     GGQFANVIGHNWGSTSRRGRFNVSLNYRLNDKIQIGVNSNAYTAKSQTFFLWGGGGIN
                     KYIPGAITGYPTVSRSVRTTIDPYFRYADMKGNSHKILMRYMRTSVDNSNNQDNGSDF
                     IYGQYQYQRNISKIKFVITTGFTGNYTVSKSQLFGNAKHTMYNMAGYLQLDKKFWNRL
                     NVTYGFRFETFKIDQAKAETKPVSRIGINVEAAKYTFIRASWGQGYRFPTIAEKFVET
                     SLSTDLGIYPNPNLTSETGMSAELGIKQGVKLGRNLNAYVDVAAFYQEYKNMMEFNIQ
                     YFQGKGVGFSSVNIGNTRIFGTEVTLMGEGKLFNKFPTTLVAGYTFIIPQYKQFDRTL
                     TKDYTNYNVLKYRFRHQVQTMWDISFGPFDFGASLQYFSFMENFDNILSSVSPSILEY
                     RFSHLKKNWQNKDSKNQFKGDVVLDLRAGINFGKEKNYKLMFTVKNITNREYTLRPSL
                     IEAPRNYGFRFDATF"
     gene            5353..>6635
                     /locus_tag="PLS16_09875"
     CDS             5353..>6635
                     /locus_tag="PLS16_09875"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNJ61431.1"
                     /translation="MNRYLFILLLHVCTVQTTFAQNIIKGSIIDSKTKEAIIGANVDC
                     GTVSTVSDFDGNFEINLPDGQYTLKVSYVSYSNFQKEITLTNNQVENINIVLEEEAKN
                     LLGQDLVITGSLFEKRASEEVISIEVVKPQAILNMNAIRIDEVARKVSGLIIADGQAS
                     IRAGSGWAYGVGSRVMFIVDGQPILSPDRGDIKWSILPTEAIGQLEVLKGASSVLYGS
                     SAMNGTIHLQTVKPTKKPLTRFTMFQSFLAPPKDRVKKWWDFPVPSFGGTFLRAHKPR
                     ETFEYFVSGSAYLNNTQFQNGLEYMARINFRTKWTNPKNSKMSYGLAGSFMYNREYEV
                     FFWQNGTDGAYKAGADNAFNNIRFTVDPFFTIYDKKNNKHEIKTRSYFNRPSFATKTF
                     LETIDYNFNKNFTKIGLNLIVGANEQMLVINVPAF"
BASE COUNT         2321 a          976 c         1137 g         2201 t
ORIGIN      
        1 cagcaactgg acgagaacat gctgtagtcg cacctaatac atttgctaaa tcttcgataa
       61 ttccccagtt ttctggacct ttcataccac gaccagcact tactaccaat tctgcttcag
      121 gaagtggagc aacgttaccg ccctctctct taacatcttt aacagtaact tttgatgctg
      181 caggagcaat atctgaagct tcaactgttg cactaccaga accttttgta gcacccactg
      241 agtttggaac aaatgcaatg actttattat cgcttgtaat tttcataaat gcaaaagctt
      301 ttcctgaaaa tgcagttttc ttaactacga aatctgcatc tggtaaactt actgcaccac
      361 tgataatacc agcatttaaa cgaacagcaa ctctaggagc tactgctttt cctgttaatg
      421 tttgaggaat aattacaact tttgcaccgt ttgcagcagc agcatcgctt accgcttttg
      481 cccaagcacc agcatcaaag ctatcaaaac ctgcattgtt tgcatgtaca actttttttg
      541 caccataaat tcctaattct gccaattcag cattgtctga agttcctaaa gctaatccaa
      601 cagcttcagt tcctaattgt gttgcaactt gtgctgcata acttgccgct tcataagaag
      661 ctttttttac ttttccagcg gctacttcta ctaaaaataa tactgccatt ttaaattact
      721 tttgcttcgt tatgtaataa ttgtaccaac tcgcctacat tatctggaga aatcatttta
      781 caagcagatt ttgcaggagg taattcatat ttaacagttg ctgttaaaga tgcagtacct
      841 tgtggcgtaa ccacatttaa aggcttggtt tttgcagcca taattccttt catattcgga
      901 atacgtgctt cagccattcc tttagcacat gagataacaa atggagcatc tacactcaaa
      961 atttcttctc caccttcgat atctctctcg attgttgcag tacttccagc aacatctaat
     1021 ttactagcat aggagatgaa aggcaaatct aacaattcag caaccatacc acccacattt
     1081 gagccgttat cgctaattgt ttcttttcct aaaagcacca aatcatagcc accagatttt
     1141 gcaacagctg caatttcact tgcaattgca taagaatcta aatcgccact tgcatcgata
     1201 cggatagcat cgtcagcacc aatagctaat gcttttctga tagtagcatc atttgctgct
     1261 ccaccaacgt taacaacagt aactgttcca ccgtttgctt cttttaattc taatgcacgt
     1321 actagtgcat accactcgtc gtaagggttc aaaatccact gtaccttatc tgtgttgaac
     1381 tttgtatcgt tgtcaacaaa agaaattttg gtagtcgtgt ctggaaccaa gcttatacaa
     1441 actaaaaatt tcataacctt gtttttttaa ttaataattt aattttgatg ctactaattt
     1501 agttcatttt tataaattca atgcatgcat ttaatccgaa tacaacaatt ggaagaaatt
     1561 gtaagaaaaa aaccacaaga tttgtttgct atttttgctt tagcaaaaga atatgagaaa
     1621 atagataaaa ttaaaaatgc catagaatta attgaaaatc ttttagttat agatttaaat
     1681 tatcttggag catattattt tttaggtaag ctttatgaaa aaaatgaaca gattaataaa
     1741 gcattaaata tttatgaagc aggaatatta attgctcaaa aacaaaaaaa tcttcatacg
     1801 cttagtgaac ttcgaaatgc aaaactaaat ttggagctag aggatattta atgaagggcg
     1861 tatatctttt taacccgtat tgtgcaatag gattcgttat gaaagtgaaa acaccaataa
     1921 aatataaact tttgtgattg aaacatagca acctctatga taaatgagca aaagttagcg
     1981 tagcgttata ttttgcaggc gttttcgatt gtaatagaat atctatagca taattcgggt
     2041 ttaacttata aaagggtaat ttgtaaggat tttttacatt taacaatttt tttttctatt
     2101 aagcgtcttt aatttaaaat ataattgtag ttttgtccta ataaattaaa ttaacttaat
     2161 atgagaaaaa acgttttatt tttatctatg gtagctttag catttgctat gattttacct
     2221 tcatgtaaaa atgaagattc ttacgcatgt gagcaatctc aatttgttgg aacttacaac
     2281 ggttcgcaca atttatcaat taatcaacca attgaattat caattccaat taatgataaa
     2341 attgttgttg ctgcaattgc tggctctact gattctgtta ctatccaatc acaaacttta
     2401 ggaattacat taagagctca agtaaaagat tgtaagatta gtattcctgc aagtgtttct
     2461 gaatttactt atgatttagg tggaatgatt ggacgtgtta aaatttctgg agtaaatgca
     2521 actttgactg ttagaaaaaa tacaactaaa ggtcaagttg aatcagtaat ttctgttcaa
     2581 acaggtacag ctaatacttt agacaatgat ttaattgatg atttaagtat tgctaatact
     2641 gaattaccag gtatttttaa ataattaaaa tctatcttta gatattaaaa aaaatagctc
     2701 caaaattttg gagctatttt tattgttcaa ttatgaaaat aaaactattt tagcggaaaa
     2761 ttaaataaat ttaacatgag gatactactc gtaacattac tatttacaat gtttagctta
     2821 atgtcaatgg ctcaaaatgg aattttaaaa ggaaaaatta cagatgccaa gtcaggtgaa
     2881 gaactaatag gagtaaacat tggtttaaaa ggtacaactg aaggaactac tacagatttt
     2941 gaaggaaact atgagctttc actaccttca ggagtccatt taatagtttt aagttatatt
     3001 ggctatgaag aaaaaagtga aacggtaaca attaaagaaa atgaaacaat tactttagat
     3061 attaagttag gagaatcaag aagcttatta gatgaagtgg tcgtaacgac tagtaaattt
     3121 gaaagaagaa taggagagga aacagtttct attgatgtta ttaagccaca aatgatagaa
     3181 aaacaaaact tactcgatgc tggagatgcc gtaaaaagaa gtccaggagt tacagtagtt
     3241 gatggtcaac caaatattcg tggaggttct ggttggtctt atggtgctgg ttctagagtt
     3301 ttgattttag tggatgatat gccggcatta caacctgaag ctggttttgt gtcttgggat
     3361 caatttccaa ttgagaatat tggacaaatt gaagttatta aaggagcagc ctcggcatta
     3421 tatggttcat cagcgatgaa tggtattata aatatacgta cagcctatcc aacttcaaaa
     3481 ccatatttta aattttcagt ttttggtgga gtgattgaca atccaaatcc taatgaaaaa
     3541 attggagttg tagatagttt tggaaatttt actggtgttt tttcagaaac aaaagtcaat
     3601 aaaaaatggt gggatttaga tagtattact tttaatggcg gagctatcgc taatggagct
     3661 attaatgtga atgatacaac atttgtgaat aattcaaaac aaagacctca taattatggt
     3721 ttttcttgga attacagaga aaaattattg aaaaataaaa aattagactt catttttggt
     3781 ggacaatttg ctaatgtaat tggacataat tggggaagta catcacgtag aggaagattt
     3841 aatgttagct tgaattatag attgaatgat aaaattcaaa ttggtgtaaa ttcaaatgct
     3901 tatactgcaa agagtcaaac gttcttctta tggggcggtg gcggaatcaa taagtatatt
     3961 cctggtgcaa ttactggtta tcctactgtt tcacgttcag ttagaacaac gattgaccct
     4021 tattttagat atgcagatat gaaaggaaac tctcataaaa ttttaatgag atatatgaga
     4081 acttcggttg ataattcaaa caaccaagat aatggttcag attttattta tggtcaatac
     4141 caatatcaaa gaaatatttc taaaattaaa tttgtgatta caacaggttt tactggaaac
     4201 tatacagttt ctaaatcaca attatttgga aatgctaagc atacgatgta taatatggca
     4261 gggtatttgc aattagacaa aaaattctgg aatagattaa acgttactta tggtttcaga
     4321 tttgagacat ttaaaattga ccaagctaaa gctgaaacaa aaccagtatc aagaattggt
     4381 atcaacgtag aagctgcaaa atatacattt attagagctt cttggggaca aggatataga
     4441 ttcccaacca ttgcagagaa atttgttgaa acaagtttaa gtacagattt aggcatctat
     4501 cctaatccaa acttgacttc tgaaactggt atgtcggcag aattgggtat taaacaaggt
     4561 gttaaattag gcagaaattt gaacgcttat gttgatgttg ctgcatttta tcaagaatat
     4621 aaaaatatga tggagtttaa tatccaatat ttccaaggta aaggtgttgg tttttcatca
     4681 gtaaatattg gaaatactag aatttttgga actgaagtaa ctttaatggg tgaaggtaag
     4741 ttatttaata aattcccaac tactttagtg gctggctata cttttatcat tcctcaatat
     4801 aagcagtttg atagaacact tacaaaagac tatacaaatt acaatgtgtt gaaatataga
     4861 tttagacatc aagtacaaac catgtgggat ataagttttg gtccttttga ttttggtgca
     4921 tctttacaat actttagttt tatggagaac tttgacaata tattaagttc agtatcacct
     4981 agtattttgg aatatagatt ctctcatttg aaaaagaact ggcaaaataa agattctaaa
     5041 aatcaattca aaggtgatgt ggttttggat ttgagagcag gtattaactt tggtaaagaa
     5101 aaaaattata aattaatgtt tacagttaaa aacattacca acagagaata tacgttaaga
     5161 ccgtcattga tagaagctcc aagaaactat ggctttagat ttgatgcgac attctaatat
     5221 ttttcattta gctattttta gttagtaaaa tagagtatcc gtactgaaaa gtgcggatat
     5281 ttttatttta tgtatttggt aatattttat ttgcatttat taacgtagtt ttgcatccaa
     5341 attgatttaa taatgaatag atatcttttt attttattac tacatgtatg tacagtacaa
     5401 acaacttttg ctcaaaatat tattaaagga agtattatag attctaagac gaaagaagct
     5461 attattggtg caaatgttga ttgtggtaca gtttcaactg tttcagattt tgatggaaat
     5521 tttgaaataa atttaccaga tggacaatat actttaaaag taagttatgt gagttattcc
     5581 aattttcaaa aggaaattac tctaacaaat aatcaagttg aaaatataaa tattgttctt
     5641 gaggaagagg ctaaaaatct attaggtcaa gatttggtta ttacaggttc actatttgaa
     5701 aaaagagcaa gtgaagaagt aatatcaatt gaagtcgtga agccacaagc aattctaaat
     5761 atgaatgcta ttagaattga tgaagttgca agaaaagtat cgggtttgat aattgctgat
     5821 ggacaagcaa gtattcgagc aggaagtggt tgggcttatg gcgtgggttc tcgggtaatg
     5881 tttatcgtag atggacaacc gatcttatct ccagatagag gcgatataaa atggtcaatt
     5941 ttaccaacag aagcgattgg tcaattagaa gtattgaaag gagcatcttc agtgctttat
     6001 ggctcttcag caatgaatgg aacaatacat ttgcaaacag taaagccaac caaaaaacca
     6061 ttaaccagat ttactatgtt tcaatcattt ttagctcctc caaaagatag agtgaagaaa
     6121 tggtgggatt ttcctgtacc atcatttggt ggaacatttt tgagagcaca taaacctaga
     6181 gaaacgtttg aatattttgt aagtggtagt gcttatttga ataatactca atttcaaaat
     6241 ggattggagt atatggcaag aattaatttc agaaccaagt ggacaaatcc aaaaaacagt
     6301 aaaatgtctt acggattagc gggtagcttt atgtataata gagaatatga ggtctttttt
     6361 tggcaaaatg gcacagacgg agcatataaa gctggtgctg acaatgcttt taataatatc
     6421 agattcacag tagatccatt ttttacaatt tatgataaaa agaataacaa acatgaaatt
     6481 aaaaccagaa gttattttaa ccgaccttct tttgctacca aaacattttt agaaaccatt
     6541 gattataact tcaataagaa ttttacaaaa ataggattaa atttaatcgt aggagcaaat
     6601 gagcaaatgt tagtcataaa cgtacctgca ttttt
//
LOCUS       DAOQCW010000124         6598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_4724_length_6598_cov_5.205716, whole genome shotgun sequence.
ACCESSION   DAOQCW010000124 DAOQCW010000000
VERSION     DAOQCW010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6598
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4724_length_6598_cov_5.205716"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..1094)
                     /locus_tag="PLS16_09880"
     CDS             complement(6..1094)
                     /locus_tag="PLS16_09880"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HNJ61432.1"
                     /translation="MSEKLNIAIVFGGKSPEHEVSIISARNIYHAIDKEKFEIQLIGI
                     SPNGQWFLENNANLQDKNCVIGKDNETLCVQFGNPEKAFFNIANKTYLPKVDAAFLIT
                     HGPNGEDGSLQGIFVHLDIPFVGPSVLSSAVSMDKDFTKRILRDSGIGHAKGVTIYKQ
                     DRYNTNYETLKVELGDVMFVKPCNMGSSVGVSKVTNKEEFENALDEAFIFDRKVLIEK
                     GVVGREIECAVLGNENAEASTVGEIVMNYGIYDFDNKYINDAAAQLHIPAKGLTTAQM
                     DKIRNTALAAYKALDIEGLSRIDVFLTEDDEVIINEPNTLPGFTSVSMYPKLWETSGL
                     PYSDLIEKLIDLAIQRHQRETQLKRTRL"
     gene            1228..1986
                     /locus_tag="PLS16_09885"
     CDS             1228..1986
                     /locus_tag="PLS16_09885"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNJ61433.1"
                     /translation="MNKYILNLLYVIALSFVLLFATLSFLKWYTKHGESITVPNIEGK
                     SFYEAINILKNNDLDYMVIDSTFDAKKPPLAILYQNPLEGAKVKTGRKIYVTMNAAYP
                     PSVKIPSIIDNSLRQAEIILNSWGINIGQLIYIPDLAKNAVLGIKYKGEDVKPGKVIP
                     KGSSIDLVLGDGIGSVVTEVPPLVGLTVLEAKAVLEAVHIPMGELIADGPIKDTMQAF
                     IYNQDPMYGSLGKLNEGNTVKLFISDTPVEFKQP"
     gene            2002..3900
                     /locus_tag="PLS16_09890"
     CDS             2002..3900
                     /locus_tag="PLS16_09890"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNJ61434.1"
                     /translation="MRQILYIALCSIIFTLSAQESLMPFPKYGQLSETTNKKYIQQKQ
                     KQRIAALNLPFIEDFYQSAIYPDVTKWQDNLVYVNQTFDLHPVSIGMAIFDGLDASGQ
                     PYSNVPNSTGIADRLTSQSLDLSGLSKTDNVLMSFFYAYNTLGETPENNKDFLYLQFL
                     DERNEWQTVWQKTPNKNDDSTNFKQIFIAIDTPFLHNNFQFKFYNVGSLYGLSDIWAL
                     DYIRIDKDRDSIFDSNIKDMAFQYPAPSLLKRYYNMPYNHFDSTQMADTILLSVKNNF
                     INPTTDFVDRYTAKVLNVGATLNIYNGPSRDFLPLTENKIPYPLFQIPETYTDDTIVI
                     ETNFSFDVTAEDISIPEITNNNKLKQTQVFSNYFAYDDGSPERAYWVRDVDNYKMAVK
                     YELKVPDTLRAIKYQFTQVKSDIKNAKFSVVVWKNVEFNSTNEQIIYKEDNLKMENLV
                     KEFGFDTINGLLYYNIKPEYIVDNNYSFPLVLTDSFLVGVIVQDRNTLSVGFDRNNNK
                     QQYNYFVSPVGDYANKWLKSDIKGTMIMNPVFGKELPWQLTPIKNNNTYAIKVFPNPA
                     QKYLHLSGIKEKTNVQITDLNGKLIQSFNTDIDRIINIENLASSIYILSVRNLETQAI
                     GTIKFIKN"
     gene            complement(3897..5663)
                     /locus_tag="PLS16_09895"
     CDS             complement(3897..5663)
                     /locus_tag="PLS16_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNJ61435.1"
                     /translation="MAAKRLFDILSEQLEKNPNNKFLSAKVDGVWKDYSFTETQFYAN
                     QVSQLLLNLGLKKDDKIAIISSNRPEWNFTDLGAQQLGVTAVPMYPTISEHDYEYIFN
                     DAGIQYAFVGDKTILNKVQPLLGKSKSLKGIYIFDKENGATYFWDALPKNIDETAIQK
                     AKDTITEEDLATIIYTSGTTGNPKGVMLTHKNIVSNIEGVRQVVHFGYGEKSLSFLPL
                     CHSFERMVFYAYLSYGIHIHYAESLEKIADNLQEVKPYCFTTVPRLLEKVYEKIMAKG
                     ADLTGFKKKLFYWAMDVAAEYELGVNKGLWYNIQLEIARKLVFTKWQEALGGNVKFII
                     TGAAAMQPRLITLFTAAGVNVIEGYGLTETSPVISCNLVEETKRCIGTVGIPIPTVQV
                     KLADDGEIIVKGDSVMKGYYGRQDLTDEVIDKNGWFHTGDIGTWIKRNGNEFLKITDR
                     KKELFKTAGGKYIAPQAIENKFKESNFIEQLMVVGGDDKKYVAALIIPSFPQLVAWAK
                     ENGISDLTNHSLVSNAKILELFENEIKQLNLNFGQWEQIKKFALLANDWTQETGELTP
                     TMKLKRKFINEKYSKEISALFN"
     gene            complement(5679..6230)
                     /locus_tag="PLS16_09900"
     CDS             complement(5679..6230)
                     /locus_tag="PLS16_09900"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HNJ61436.1"
                     /translation="MNNLGILKRENTTLLVVDIQERLMPVIHNAEQIFANANRLIKGA
                     EMLNVPIVVTEQYPKGLGNTCAEIELPELPAVFEKTCFSCMLSEPVLGQLEQLERKSL
                     IICGVEAHICVLKTALDALKLGFEVHIVADAVSSRTLENKQIALDRLRQAGAFIVSTE
                     MVLFQLIDEAGTEQFKSISKLIK"
     gene            complement(6246..>6598)
                     /locus_tag="PLS16_09905"
     CDS             complement(6246..>6598)
                     /locus_tag="PLS16_09905"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNJ61437.1"
                     /translation="VWIIYGDGSAGYSLMENDTYVRHNLPVISLIGNDACWSQIARDQ
                     VDFLGSDCALNLAHSNYEMIGKAFGADGARVENIAEFKNAVKDALEKSRKGIPYTLNA
                     IIGRTEFRKGSISV"
BASE COUNT         2286 a         1166 c          863 g         2283 t
ORIGIN      
        1 aaaaatcaca acctagttct tttgagttgt gtttctcttt gatgtctttg aatggctaaa
       61 tcaattaatt tttcaattaa atcggaatag ggcaatcctg atgtttccca caatttagga
      121 tacatactta cagaagtaaa gccaggcaaa gtatttggct cattgattat cacttcatca
      181 tcttcggtta aaaacacatc tattctactc aaaccttcaa tatctaaagc tttataagct
      241 gccaaagcag tatttctgat tttatccatc tgagctgttg tcaaaccttt tgcaggaata
      301 tgtaattgag cagctgcatc attaatgtat ttattatcaa aatcataaat gccataattc
      361 attacaattt cgccaacagt actcgcttcg gcattttcat tacccaaaac agcacactca
      421 atttctctac caacaacacc tttttcaatc aacacttttc tatcaaatat aaatgcttca
      481 tctaatgcat tttcaaattc ctctttattg gtaactttac tgacaccaac tgaagaaccc
      541 atattacaag gctttacaaa cattacatcg cccaattcta cttttaaggt ttcatagttg
      601 gtgttatatc tatcttgttt gtatatggtt acaccttttg catgaccaat gcctgaatct
      661 cttaatattc gtttggtaaa atccttgtcc atagaaactg cagatgataa tacagatgga
      721 ccaacaaaag gaatgtctaa atgtacaaaa ataccttgca gagaaccatc ttcaccatta
      781 gggccatgcg taattaaaaa ggctgcatct actttaggta aataagtttt gttggctata
      841 ttaaaaaatg ctttttcagg attgccaaat tgaacacaca aagtttcatt atcttttcca
      901 attacacaat ttttatcttg aagatttgca ttattttcta aaaaccattg accatttggg
      961 ctgataccaa ttaattgtat ctcaaatttt tctttatcaa tagcatgata aatatttcta
     1021 gcagaaatta tagacacttc atgttcagga gattttccac caaaaacaat agcaatattt
     1081 aatttttcag acattaaaaa aacaatttgc aacaaaagta cgagtaattt gtgcaatctt
     1141 aattacttat acacaattta ttaagatttt ttttgaactt actacaagtt aaatatatat
     1201 atttgaggtt tattttagat acttttaatg aataaatata tcctcaatct attatatgta
     1261 attgcactta gttttgtttt gctatttgca acgctatctt ttctaaaatg gtacactaaa
     1321 catggcgaga gtattacagt tccaaatatt gaaggcaaat ccttttatga agccattaat
     1381 attctaaaaa acaatgattt agattatatg gtcatcgatt ctacttttga tgcaaagaaa
     1441 ccacctttag ctattttata ccaaaatccg cttgaaggtg ctaaagttaa aacaggtaga
     1501 aaaatctatg taacgatgaa tgctgcctat cctccgagtg taaaaatccc ttcaattatt
     1561 gataattctc tcagacaagc agaaattatt ttaaatagtt ggggcattaa tattggacaa
     1621 ttaatttata ttccagattt agctaaaaat gcagttttag gaattaaata taaaggtgaa
     1681 gatgtaaaac ctggaaaagt tatacccaaa ggcagcagta tagatttagt tttaggcgat
     1741 ggtattggta gtgtcgttac agaagtaccg ccattggttg ggttaacagt tttagaagct
     1801 aaagctgttt tggaagctgt acatatccca atgggcgaac tgattgctga cgggcctatt
     1861 aaagatacca tgcaagcttt tatttataat caagatccaa tgtatggttc tttaggtaaa
     1921 ttaaatgaag gcaatactgt aaagttattt atttcagata ctccagttga gtttaaacaa
     1981 ccctaaattt aatttattct aatgaggcag attttataca tagcattatg ttcaattatt
     2041 ttcacattgt ctgctcaaga aagcttaatg ccttttccaa aatatggaca gttgagtgaa
     2101 actaccaata aaaaatacat tcaacaaaaa caaaaacaaa gaatagctgc attaaattta
     2161 ccatttattg aagattttta tcaaagtgct atatatcctg atgttacaaa atggcaagat
     2221 aacttagttt acgtaaacca aacatttgac ttacatcctg tttctattgg aatggctatt
     2281 tttgatggat tagacgcaag tggacaacca tacagtaatg ttcctaactc tactggaatt
     2341 gctgaccgac taacttctca atctttagat ttatctggct tatcaaaaac agataatgta
     2401 ttaatgagtt tcttttatgc ctataacaca ttaggcgaaa cacctgaaaa caataaagac
     2461 ttcttatatt tacagttttt agatgaacgc aatgaatggc aaacagtttg gcaaaaaaca
     2521 cctaataaaa atgatgactc aacaaatttt aaacaaatct ttattgcaat agacacaccc
     2581 tttcttcata ataatttcca attcaaattt tataatgtag gtagtttata tggtttgagt
     2641 gatatttggg cattagacta tattagaatt gataaagaca gagattcaat tttcgatagt
     2701 aatatcaaag atatggcatt tcaatatcca gcaccaagcc tgttgaagcg ttattacaat
     2761 atgccatata atcattttga tagtactcaa atggctgata caattttact ctccgtaaaa
     2821 aataacttta tcaatccaac tacagatttt gtagatagat atactgcaaa ggtattgaat
     2881 gttggagcaa cacttaatat ttacaatggt ccatctcgag atttcctacc tctaactgaa
     2941 aacaaaattc cttatccatt atttcaaata ccagaaacct atactgatga tacaatcgtt
     3001 atagaaacta atttttcttt tgatgtaact gctgaggata taagcattcc tgaaataacg
     3061 aacaataata aacttaaaca aactcaagtt ttttctaatt attttgctta cgatgatggc
     3121 tcacctgaac gtgcttattg ggtacgagat gtagataatt acaaaatggc agtaaaatat
     3181 gaattaaaag taccagatac tttacgagct ataaaatatc aatttacaca agtgaaaagc
     3241 gatattaaaa atgcaaaatt tagtgtggta gtttggaaaa atgtcgaatt taattcaact
     3301 aacgaacaaa ttatttacaa agaagataat ctaaaaatgg aaaacttagt taaagaattt
     3361 ggctttgaca cgattaatgg attattatat tacaatatta aacctgaata tattgttgat
     3421 aataattatt cttttccttt agttttaaca gattcttttt tagtgggcgt aattgtacaa
     3481 gataggaata cattaagtgt tggatttgat agaaataata ataagcagca atacaattac
     3541 ttcgtcagtc cagttggtga ttatgccaac aaatggctaa aatctgatat taaaggaaca
     3601 atgattatga atccagtatt tggaaaagaa ttaccttggc aattgactcc tataaaaaat
     3661 aacaacactt atgcaataaa agtatttcca aatcctgctc aaaaatattt gcatttatca
     3721 ggaataaaag aaaaaaccaa tgtgcagata acagacctca atggaaaact aattcaatct
     3781 tttaacaccg acattgacag gataattaat attgaaaatt tagcatcttc tatatatatt
     3841 ctatctgtca gaaacctaga aactcaagcg attggaacta ttaaatttat caaaaactaa
     3901 ttaaataaag cactaatttc tttactgtat ttttcattaa taaacttacg tttcaatttc
     3961 attgtaggtg ttaattcgcc tgtttcttgt gtccagtcat tagccaataa agcaaacttt
     4021 ttaatctgtt cccattgacc aaaattcaaa tttagttgtt taatttcatt ttcaaataat
     4081 tccaaaattt tagcatttga aaccaaagaa tgattagtca aatctgaaat accattctct
     4141 tttgcccaag ctactaactg cggaaatgat ggaataatta aagctgcaac atatttttta
     4201 tcatcgccac caacaaccat taattgctca atgaaattag attccttaaa cttattttct
     4261 attgcttgag gtgcaatata tttacctccc gcagttttaa acaattcttt ttttctatct
     4321 gtaattttca aaaactcatt cccatttcgt tttatccaag taccaatatc tccagtatga
     4381 aaccatccat ttttatcaat aacttcatct gttaaatcct gacgaccata atagcctttc
     4441 atcacactat ctccttttac aattatttcg ccatcatctg caagttttac ttgtactgtt
     4501 ggaattggaa tccctactgt gccaatacat cttttggttt cttccaccaa attacaagaa
     4561 atcacaggcg atgtttctgt cagaccgtaa ccttcaataa catttacgcc agctgctgta
     4621 aataacgtga ttaatctagg ttgcatagct gctgcaccag taataataaa tttcacatta
     4681 ccacccaaag cttcttgcca ttttgtaaat actaattttc tagcaatttc caattgaata
     4741 ttataccata aacctttatt tacacccaat tcatattctg ctgcaacatc cattgcccag
     4801 taaaacaatt tctttttaaa accagttaaa tcagcacctt ttgccattat tttctcataa
     4861 actttctcta ataagcgtgg tactgttgta aagcaatatg gttttacttc ttgtaaatta
     4921 tctgctattt tctctaaact ttcagcataa tgaatatgta ttccataact caaataagca
     4981 taaaaaacca tacgctcaaa actatggcaa agcggtaaaa aacttaatga tttttcgcca
     5041 tagccaaaat gtactacttg tctaacacct tcaatattag aaacgatatt tttgtgtgta
     5101 agcatcactc cttttggatt gccagttgta cctgaagtat aaattatcgt tgctaaatct
     5161 tcttcagtga tagtatcctt agccttttga attgctgttt catctatatt tttaggtaag
     5221 gcatcccaaa aataggtagc cccattttct ttatcaaaaa tataaatccc tttcaatgat
     5281 ttactctttc ccaataatgg ttgcacctta ttcaaaattg ttttatcgcc aacaaaagca
     5341 tattgaatac ctgcatcatt aaaaatatac tcataatcgt gttcagaaat tgtaggatac
     5401 ataggaacgg cagtaacacc taattgttga gcacctaaat cagtaaaatt ccattcaggt
     5461 ctattagatg aaataatggc aattttatca tcttttttca aacctagatt caacaataat
     5521 tgactgactt gatttgcata aaattgtgtt tctgtaaaac tataatcttt ccaaacacca
     5581 tctacctttg ccgacagaaa tttattatta ggattttttt ctaattgctc tgaaaggatg
     5641 tcaaataaac gttttgccgc cataatttgc ttagtttttt attttattaa tttactaatt
     5701 gatttgaatt gttctgttcc tgcttcatct attaattgaa acaaaaccat ttctgtactt
     5761 actataaatg caccagcttg tcttaatcta tctaatgcaa tttgtttatt ctccagcgtt
     5821 ctggaagaaa cagcatcggc aactatatgc acttcaaaac ctaattttaa agcatctaaa
     5881 gctgttttta aaacacaaat atgtgcttca acaccacaaa taattaagga cttacgttct
     5941 aattgttcca actgtcctaa aactggttca gacaacatac aactaaagca agttttttca
     6001 aaaacagcag gtaattcagg caattcaatt tctgcacatg tgtttcctaa tccttttgga
     6061 tattgttccg taacaacgat aggtacattc agcatttctg caccttttat caatctatta
     6121 gcatttgcaa aaatttgctc tgcattatga attacaggca ttaaacgctc ttgaatatcg
     6181 acaactaaaa gcgtagtatt ttctcttttt aatattccaa gattattcat aacaatattt
     6241 tttagttaaa ctgaaattga accttttcta aattctgttc ttccgatgat agcatttaaa
     6301 gtataaggaa taccttttct acttttctct aaagcatctt taactgcatt tttaaattct
     6361 gctatatttt caactcttgc accatcagca ccaaaagctt ttcctatcat ttcataatta
     6421 gaatgtgcta aatttaaagc acaatcgcta cctaagaaat ctacctgatc tcttgcaatt
     6481 tgagaccaac aagcatcatt gccaattaaa gaaataacag gcaaattatg gcgcacataa
     6541 gtatcatttt ccatcaaact ataaccagca cttccatcgc cgtaaataat ccaaacat
//
LOCUS       DAOQCW010000125         6488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_4865_length_6488_cov_5.457640, whole genome shotgun sequence.
ACCESSION   DAOQCW010000125 DAOQCW010000000
VERSION     DAOQCW010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6488
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4865_length_6488_cov_5.457640"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..989
                     /locus_tag="PLS16_09910"
     CDS             105..989
                     /locus_tag="PLS16_09910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HNJ61438.1"
                     /translation="MSRKWAYTRSERNALVALLAIIFILIIFKNPIINRITVYNNKID
                     STKYFTLIAQIENAKIDNNSYNTLKTSFSSFTSNKISDEYTKIEINSSTAEDFQRLKG
                     IGKVFAERIVKYRDKIGGFSSLEQLKEVYGIHDTVYQKFKSNIYVKPVSKKQIIITKR
                     IIEINSASQDDFESLNGIGKVFAQRIIQFREKLGGFYSKEQLKDVYGIHDTVYQKIKE
                     QIKITPVQFTRKINVNTATYEELTSNPYLFSTVAKQIIGYRTKVKPFNSIEDLQKLYY
                     IKDHPEYYDKILPYISID"
     gene            1049..1768
                     /gene="cobA"
                     /locus_tag="PLS16_09915"
     CDS             1049..1768
                     /gene="cobA"
                     /locus_tag="PLS16_09915"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNJ61439.1"
                     /translation="MKERKKFILVGAGPGDPDLITIKGTKALEQADVVLYDALIDESL
                     LEYAPYSEKKIFVGKRMGEHYASQDEINQMILENLEAYHCVVRLKGGDPFIFGRAQDE
                     INFVLANRDDVDIEVIPGISSAVGLPSLHQIPLTQRGVNESVWLVTGTTEKCEMSKDI
                     EFAAQSTATVVILMGMNQLENIIHTFKQHRKVNTKVTVIQNGSLPDEQKVIGTLENIQ
                     QLVAENEIKSPAVIIIQNSNS"
     gene            1797..2348
                     /locus_tag="PLS16_09920"
     CDS             1797..2348
                     /locus_tag="PLS16_09920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HNJ61440.1"
                     /translation="MKLESLNLLIVGGGKVCMEKLNFILKSSPDAVITIVADKISETI
                     KTQYQNNKKIHLIEKAFEPDDVLGFQIVIAATNNKATNLEIYKAAKSNNILINVADTP
                     ELCDFYLGSIVSKGDLKIGISTNGKSPTMAKRLREFFEATLPEHIDELIVNMNVYREK
                     LKGNLAEKATRLNELTKPFNENL"
     gene            complement(2343..3752)
                     /locus_tag="PLS16_09925"
     CDS             complement(2343..3752)
                     /locus_tag="PLS16_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61441.1"
                     /translation="MINTIKYYTFFFAIVFILFAFYNIPQIINFPAQGAHLWRQADGL
                     AMVWNYKLFNLPFLQTETFNLLSTEGKGIAEFPIFYFFAAQFVNPEKALRILHFVVLV
                     LGFFAVYRLAYFYLRNNFYTVAVLLLFASSPLIIFYGINFIIDVPALCFSLFAWSVWL
                     VDNNKKYSTLLSLSLFSFAALLKAVHVLNFIVLFYLLYKEKQINFKNIVLFSIAICIP
                     TIWYFYAKWYNHNYHNDYLFLSIQPIFMMSFYDIGLAIWRIIVSWSATYFWRPTSVIL
                     IVVLSYYLLKKEKNELSIMIVYTFVLDVIFALLFLEKLIEHEYYYPIFYINIVFVLIG
                     FLYFLQKQHKQNYINIALLIFIGINLFYCKNNTAEKQTVQRIDSKLISVDFQNFLNKN
                     QCTQKKTVFCYDDFSVNQSLYAIKRKGITQYNQNWKEFILKNKIDFVLVSEQSIDKII
                     KSVYPKNIYTYKNYKLIAL"
     gene            complement(3896..4255)
                     /locus_tag="PLS16_09930"
     CDS             complement(3896..4255)
                     /locus_tag="PLS16_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61442.1"
                     /translation="MHILIDNKQAGYLDFKSPEKKIEIGDSNLEKNLTIKLSVFKKTI
                     PVKANSTIIIDQNKSFQILFVLMLISAGLFFILPNFISSMYLQYALLVCPVLYVLSMF
                     YYTFLGRDNLLEIKVID"
     gene            complement(4318..4755)
                     /locus_tag="PLS16_09935"
     CDS             complement(4318..4755)
                     /locus_tag="PLS16_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61443.1"
                     /translation="MSNFGDSLFMYNVDSPEDFISKYDNFTDSIKNQNNVIGNVVVEL
                     EDVLTNFEAEDEIKTEIHKIFDKYITLADEMDKQKILVITGTALGSVEYWNNNIQLIE
                     SNEQNQRINKLKQVMEFVLADATCGAYGGPAGVICSGLMALSW"
     gene            complement(4760..5014)
                     /locus_tag="PLS16_09940"
     CDS             complement(4760..5014)
                     /locus_tag="PLS16_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61444.1"
                     /translation="MTKKLKKMIAIISISGLIALTLISCEKATKALSKNDFNKQLKEI
                     SEYHNNTLDDFIKSTNNFEKANFRVNYDNLKNIVKKRVAE"
     gene            5200..5595
                     /locus_tag="PLS16_09945"
     CDS             5200..5595
                     /locus_tag="PLS16_09945"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNJ61445.1"
                     /translation="MTKAQNTFIIGNNLRRLRLDKDFNQKQMCDILNLSQSKYSLIES
                     NQKTVSLEQVEQYADALEVDIDKLLKDPTEYIFHSCNYSINGGVSNKLENKCDAEIIK
                     ALQSQLNLLQIQLDIKDQQIQQLLSLLEK"
     gene            complement(5685..>6488)
                     /gene="hflX"
                     /locus_tag="PLS16_09950"
     CDS             complement(5685..>6488)
                     /gene="hflX"
                     /locus_tag="PLS16_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNJ61446.1"
                     /translation="QVELAQLQYLLPRLKGLWTHLERQRGGIGMRGPGEKEIETDRRV
                     INDKIAKLKQDLVVLDKQDETRRKTRGELVRVALVGYTNVGKSTMMNLLSKSDILAEN
                     ILFATLDTTVRKVVIDNMPFLLSDTVGFIRKLPHHLVESFKSTLDEAKESDIIVHVVD
                     ASHENFRDQIDVVNQTLADLKVTEQPRLIVFNKMDLYKEKHFDKYLPEDVKAELLLEF
                     EKNMTSMMNTNCVFISALEKENIETLRNLMILMVQDMYHKRYPYRSNFY"
BASE COUNT         2435 a          941 c          889 g         2223 t
ORIGIN      
        1 gctagccttt tttaatactt tttaaagctg tatgcattgg ttataataaa ttaagtttat
       61 tttccgtact tttatcggac agtagtgata aagaattaaa aactttgagt cgaaaatggg
      121 catatactcg gtctgaaagg aatgcattag ttgcgttatt agccattatt tttatattga
      181 taatatttaa gaatcctatt ataaatagaa taaccgtata taataataaa attgacagca
      241 ctaaatattt tacattaata gctcaaatag aaaatgcaaa aatcgataac aatagctata
      301 acacgctaaa aactagcttt tcgagtttta catccaataa aataagcgat gaatatacta
      361 aaatagaaat caatagttca acagcagagg atttccaacg attaaaaggc attgggaagg
      421 tttttgctga gcgaattgta aaatatagag acaaaatcgg tggatttagt tctttagagc
      481 agctaaaaga agtttacggt attcacgata cagtatatca gaaattcaag tcaaatattt
      541 atgtcaaacc agtttcaaag aaacagatta ttattacaaa aagaataata gaaatcaata
      601 gtgcaagtca agatgatttt gaaagcctta atggaattgg caaagtattt gctcaaagaa
      661 taattcaatt tagagaaaaa ttaggtggtt tttattcaaa agagcaactt aaagatgtat
      721 atggcattca tgatacggtt tatcaaaaaa taaaagaaca aataaaaata acaccagtac
      781 aatttacaag aaaaattaat gtcaatactg caacttatga ggaattaact tccaatccat
      841 atttatttag cacagttgct aaacaaatta ttgggtatag aacaaaagta aaaccattca
      901 attccattga agatttgcaa aaattatact atatcaaaga ccacccagaa tattatgata
      961 agattttacc ttatatatca atagattaaa caatgtgcat tgtttatgct tggcatttgt
     1021 ctgtagaatt acataaattt acatcgaaat gaaagaacgt aaaaaattta ttttagttgg
     1081 agctggtcca ggagaccctg atttaattac aataaaaggc actaaagctt tggaacaagc
     1141 agatgtcgta ttgtatgatg cattgattga tgaaagctta ttagaatacg ctccttactc
     1201 tgaaaagaaa atttttgttg ggaagcgaat gggtgagcat tatgcttcac aagatgagat
     1261 taatcaaatg attttagaaa acttagaagc ttatcattgt gttgtacgtc tgaaaggcgg
     1321 tgatccattt atttttggtc gagctcaaga tgaaattaat tttgttttag ctaatcgaga
     1381 tgatgtcgat atcgaagtta ttcctgggat ttcatcggca gttggtttgc cttctttgca
     1441 tcaaattcca ttaacacaaa gaggcgtaaa tgaaagtgtt tggctagtta caggcacaac
     1501 tgaaaaatgt gaaatgtcta aagatattga gtttgcggca caatcaacgg ctactgttgt
     1561 tattctaatg gggatgaatc agcttgaaaa cattatccat acttttaagc aacatagaaa
     1621 agtcaataca aaagttaccg ttatccaaaa tggcagctta cctgatgagc agaaagttat
     1681 cggaactttg gagaacatac agcaattagt cgctgaaaat gaaattaaat cgcctgctgt
     1741 gattattatt caaaattcaa attcttaatt ttttgaatac gctctatcct atatttttga
     1801 aactggaatc cttaaattta ttgatagtag gaggcggaaa agtttgtatg gagaaattaa
     1861 attttatttt aaaaagctct ccagatgcag ttattacaat agttgcagat aaaatttcag
     1921 aaacgataaa aactcaatat caaaataaca aaaaaattca tttgatagaa aaagcatttg
     1981 aacctgatga tgttttgggc tttcaaatag tcattgctgc gacaaataac aaagccacaa
     2041 acctagaaat atataaggct gcgaaaagta ataatattct aatcaacgtt gcggacacac
     2101 ctgaactatg cgacttttat ttaggttcta ttgtaagtaa aggtgatttg aaaattggaa
     2161 tttctaccaa tggaaaatct ccaactatgg caaaaagatt acgagaattt tttgaggcta
     2221 cattacctga gcatatcgat gaattgattg ttaatatgaa tgtctatcgt gaaaaattga
     2281 agggaaattt agcagaaaaa gctacacgat taaatgaatt gactaagcct tttaatgaaa
     2341 atctataaag cgattaattt gtagttttta taagtataaa tatttttagg atatactgat
     2401 tttatgattt tatctatgga ttgttcgctc actaaaacaa aatcaatttt atttttaaga
     2461 ataaattctt tccaattttg attgtattgt gtaattcctt ttcgttttat ggcatacaaa
     2521 gattgattga cactaaagtc atcataacag aaaacagttt tcttctgagt acattgattt
     2581 ttatttagga aattttgaaa atctacactt attaatttgg aatcaatacg ttgtactgtt
     2641 tgtttttctg cagtattatt tttacaataa aagagattaa taccaataaa aatcaataat
     2701 gcaatattga tataattttg tttatgctgt ttctgcaaaa aatataaaaa cccaatcaat
     2761 acaaatacaa tatttatata aaaaattgga taatagtact catgttcaat tagcttttct
     2821 aaaaataaaa gtgcaaaaat aacatctaat acgaatgtat aaactatcat tatagataat
     2881 tcatttttct ctttttttaa taaataatag ctcaaaacaa caattaatat aacacttgta
     2941 ggtcgccaaa aataagttgc agaccaagaa acaataatcc tccaaattgc taatcctata
     3001 tcataaaaag acatcataaa aatcggctga atacttaaaa acagataatc attgtgatag
     3061 ttatgattat accattttgc ataaaaatac caaattgttg gaatacatat agcaatagaa
     3121 aaaaggacaa tatttttaaa attgatttgt ttctctttat ataaaaggta aaacaataca
     3181 ataaaattaa gcacatgaac tgctttcaat aaagctgcaa aactaaataa gctcaaactc
     3241 aaaagtgtac tgtatttttt attattatct actagccaaa cagaccaagc aaataaagaa
     3301 aaacagagtg ctggaacatc tataataaaa tttataccat aaaaaataat taatggcgag
     3361 cttgcaaaca acaaaagaac cgcaaccgtg taaaaattat ttctcaagta aaaatatgct
     3421 aaacgataga ctgcgaaaaa gcctaacact aaaactacaa aatgcaatat tcttaatgct
     3481 ttttctggat tgacaaattg agccgcgaaa aaataaaata ttggaaattc agcaatgcct
     3541 tttccttcag tagatagtag attgaaagtc tccgtttgta aaaatggcaa attaaataat
     3601 ttataattcc aaaccatagc taagccatct gcttgtcgcc aaagatgagc accttgagct
     3661 ggaaaattaa taatttgagg aatattatag aaagcaaata aaatgaatac tatggcaaaa
     3721 aaaaatgtat aatattttat tgtgtttatc aatgattaaa aattacagtt gttctcaaaa
     3781 aataatatag tttaaatata gcggttttaa gcattattac atcatataaa aaaatatgcc
     3841 gacagcttta actgccggca taatgataat agtttcctat aaattacttc aatgattaat
     3901 ctatcacttt tatttctaat aaattatctc tacctaaaaa tgtataataa aacatcgaca
     3961 atacgtacaa aacaggacat actaataaag catattgcag atacatacta cttataaaat
     4021 taggaagtat aaaaaacaat ccagcactta taagcattaa cacaaacaat atttgaaaac
     4081 ttttattttg atcaataatt attgttgagt ttgcttttac aggtattgtt ttcttaaaaa
     4141 cagataactt tatagtcaag tttttttcaa gattactatc tcctatttct atttttttct
     4201 ctggagattt aaaatctaaa taacccgctt gtttgttatc aatcaaaata tgcattttgt
     4261 taaaaaagct gaaatagtta ttttttctta ttactaacat aattaaatat tttaaaatta
     4321 ccaagaaaga gccataagac cagaacaaat tacaccagca ggaccgccat aagcaccaca
     4381 tgttgcatct gctaatacaa attccattac ttgttttaat ttattaatac gttggttttg
     4441 ttcattactt tcaataagtt gaatattatt attccaatat tctacagaac caagggctgt
     4501 tcctgttatt actagaattt tttgtttatc catctcatct gctaaggtaa tatatttatc
     4561 aaatattttg tgtatttctg tttttatttc atcttctgct tcaaaattag taagtacgtc
     4621 ttctaattca acaacaacat tacctattac attattctga tttttaattg aatctgtaaa
     4681 gttatcatac ttactaataa agtcttcagg agaatctaca ttgtacataa ataaagagtc
     4741 tccaaagtta gacattctac tattctgcga ctcttttttt tacaatattt tttaaattat
     4801 cataattaac tctgaaattg gctttttcaa aattatttgt actctttata aaatcatcta
     4861 gagtattatt atgatattct gaaatctctt tcagttgttt gttgaaatca tttttactaa
     4921 gagcttttgt tgctttttcg cacgaaatta aggttaatgc tattaatccc gaaatagata
     4981 ttatagcaat catttttttt aatttttttg tcattgtgta ataaatttaa tgttaaataa
     5041 taaattgaca taacccaaaa aatatttatc gcgaattata tttaatcgtt tatgtttcaa
     5101 aacaaatata tatatcagtt tcaatgttat caaactatta attgaatatt ttattttaaa
     5161 aactgaatat tttattaact ttataactaa attcatataa tgacaaaagc acagaatact
     5221 tttataattg gaaataatct aaggcgtttg cgattggaca aagactttaa ccaaaagcaa
     5281 atgtgtgata ttctcaacct ttctcagtca aaatacagtt taatagaaag taatcagaag
     5341 accgttagtt tggaacaggt cgaacaatat gcagatgctt tagaagtgga catagacaaa
     5401 ttgcttaaag acccgacaga gtatatattt cattcttgta attattctat aaatggggga
     5461 gtatccaaca aattggagaa caaatgtgat gcagaaatta ttaaagcatt gcaatctcaa
     5521 cttaatctct tacaaataca attagatatt aaagaccagc aaattcaaca gttactaagt
     5581 cttttagaaa aataattttt tgggtataat actgaaaatt ctcaatcctc ttatgttcaa
     5641 ttaatatgag tataatctct tatggtattt acaaatttta ctactcaata aaaattactt
     5701 ctatatggat aacgtttatg atacatatct tgaaccatca agatcatcaa attcctaagc
     5761 gtttctatat tttctttttc taaagctgaa ataaaaacac aattagtatt catcatagaa
     5821 gtcatatttt tctcaaattc cagtaataat tctgccttca catcttctgg taagtatttg
     5881 tcaaaatgct tttctttgta caaatccatt ttattaaaaa ctatcaatcg aggttgttct
     5941 gtaaccttca aatctgctaa agtttgatta accacatcaa tttggtctct gaaattttcg
     6001 tgagatgcat caacaacatg cacaataata tcagactctt tggcttcatc taaagtagat
     6061 ttaaaactct ctactaaatg atgtggtagt ttacgaataa aaccaactgt atcacttaat
     6121 aaaaatggca tattatcaat cacaactttt cggacggtgg tatctaaagt tgcaaagagt
     6181 atattttcag ccaaaatatc gcttttgctt aggaggttca tcatagtaga ttttccaaca
     6241 ttggtataac ccaccaaagc cactcttact aattctccac gagttttacg tcttgtctca
     6301 tcttgtttat ctaaaactac taaatcttgt tttagtttcg caatcttatc gttaatgaca
     6361 cgtctatctg tttcaatttc tttctcacct gggccacgca ttccaatacc tccacgttgt
     6421 ctttctaagt gtgtccacaa gccttttaat cttggcagta aatattgtaa ttgtgccaat
     6481 tctacttg
//
LOCUS       DAOQCW010000126         6473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_4878_length_6473_cov_5.869586, whole genome shotgun sequence.
ACCESSION   DAOQCW010000126 DAOQCW010000000
VERSION     DAOQCW010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6473
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4878_length_6473_cov_5.869586"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="PLS16_09955"
     CDS             <1..702
                     /locus_tag="PLS16_09955"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNJ61447.1"
                     /translation="IRFAQIISSVITFGLPAVMFSILKKKQSFGYGLESKRNIHWILF
                     LITPLLVLTIYPFLNLVFVLNKESFLGQLMLEQQEQYQMFIVALLKPKSIFYFLFNFI
                     LIAVIPAFLEEFFFRGTLQRLLTERLNIHLAILISSIIFSLIHFEFSAFLPRIVLGML
                     LGYAFYWSGSIWVAIFIHLFNNGLEVTLMYLKNLHLISGKLTDAPQMPTHTELVGYSI
                     LFIILSILFYRFSKR"
     gene            755..1603
                     /locus_tag="PLS16_09960"
     CDS             755..1603
                     /locus_tag="PLS16_09960"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HNJ61448.1"
                     /translation="MSDISTRIATGIVFLIVMIGGTVWGETSYLVIFSLVTMLCINEY
                     HNITNKVVQSIEHKTLYKFINTIFAAIIFLIVYLVASNRVDVIYLSFIGGFTLSWFFI
                     ELYTKSKSPFISIAVNSMGLFYIAIPFSAASFIAYYTGEYNWNLLLAVMFFAWANDSF
                     AYLFGSKLGKHKLFERISPKKTWEGFFGGIFGTVVFSYIVYLLFPEWSHQDIAYKHYF
                     ILAILTSIASTYGDLAESMIKRNLEIKDTGNTLPGHGGFLDRFDGLIFSFPVATLYLT
                     FISDLK"
     gene            1643..2305
                     /locus_tag="PLS16_09965"
     CDS             1643..2305
                     /locus_tag="PLS16_09965"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HNJ61449.1"
                     /translation="MAVTYIHKEGRTIIATSVVIVALINIISIYVLKLPNLVQNIILS
                     FTLFVLFFIFYFFRNPKRNILTDDNIIIAPADGKVVVMEEVYEPEYFEDKRLQVSIFM
                     SPSNVHVNRVPIAGEVKYAKYHDGKYLVAWHPKSSTENERTSIVIDNGSIDVLMRQIA
                     GKVARRIVYYVKEGDIVEQGKDFGFIKFGSRVDLFLPLDTKVNVKLGQKVKGGITIVG
                     EL"
     gene            2330..2737
                     /locus_tag="PLS16_09970"
     CDS             2330..2737
                     /locus_tag="PLS16_09970"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNJ61450.1"
                     /translation="MKTAVIRRATFNAAHQLWNQHWTTEKNNEIFGLCANPNFHGHNY
                     VLEVKVIGEIDLDTGYVIDLKILKSIINDCVLEKMDHRNLNLDVAEFEHLNPTAENIA
                     MVIYNIIREQLEPKFDLKIKLFETQNNIVEYPA"
     gene            complement(2777..3682)
                     /locus_tag="PLS16_09975"
     CDS             complement(2777..3682)
                     /locus_tag="PLS16_09975"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HNJ61451.1"
                     /translation="MKKRFLLYFLLILFTCSTFAQELNCEVSVISPKNQDIDPIKFKN
                     MEKAIKEFMNGRKWSNYTFKPFERIKCNIIVNISDSPSEGVYKASAIIQSQRPVFNSN
                     YNTMLLTYQDMDFNFTYEDLQILDYNDNSYSSQLTSLLAFYANLILAYDGETFAENGG
                     EVFFQKALTICNNIPANERSKYKGWSKLDGNRNRFMLVDAWLNPRYKDFRRAFLIYHF
                     NGLDKMYDDVVVARNIITTSISLFQKINKDNPVLMSLRLLMETKREELQNIYSKADMT
                     ERSMIVPILSYLDPTQAEKYKSLLK"
     gene            complement(3679..4881)
                     /gene="coaBC"
                     /locus_tag="PLS16_09980"
     CDS             complement(3679..4881)
                     /gene="coaBC"
                     /locus_tag="PLS16_09980"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNJ61452.1"
                     /translation="MNLSGKKIIVAVSGSIAAYKSAYLVRLLIKQGAYVQCVITESAT
                     QFIAPLTLATLSKNECLVDYTNASKTNWNNHVNIALWADAIIVAPASMNTIAKMANGL
                     CDNLLLGIYFSARCPVFIAPAMDEDMWHHPSNQKNIQTLVAFGHHLLEVNDGELASGL
                     IGKGRMAEPEEIIETLNTFFTEKKTPSKLAHQKILITAGPTYEPLDPVRFIGNHSSGK
                     MGLALAKAAHQRGAEVTLIIGQNNLVIPEYIKTIPVLSAKEMYQACLENFEQQYICIF
                     AAAVADYTPIDVAEQKIKKSDETLTLKLKKNVDIAFEFGKIKQAHQISVGFALETENE
                     EEHAKQKIKKKNFDLIVLNSTNDNGAGFRFDTNKITIIDKTGKVQTYNLKSKDETAND
                     ILNAIELL"
     gene            complement(4886..5209)
                     /locus_tag="PLS16_09985"
     CDS             complement(4886..5209)
                     /locus_tag="PLS16_09985"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNJ61453.1"
                     /translation="MNKKEEVVKTQSAYIETRDLEKIGAVTGNLYKSINAISKRSEQL
                     NTRLKEELHRKLEEFATVSDNLEEVFENREQIEISRYYEKLPKTSIMALAEFLNGETY
                     VEDKK"
     gene            complement(5211..6023)
                     /gene="bamD"
                     /locus_tag="PLS16_09990"
     CDS             complement(5211..6023)
                     /gene="bamD"
                     /locus_tag="PLS16_09990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNJ61454.1"
                     /translation="MPLRIIYTFVALLFFLVAFGQKPSKVSKSNDIESQKAKANEYFE
                     KKKYDKALPIYELLLTVLKGQQSVEEIYFKYAKAQYLSGSYELGAFYLKSFYNTYYNS
                     KNAEEAAFLEAVCYYKQSPRYELEQVNTQKAIAIFQAFIDKYPKSSYMQEANDKMDEL
                     RAKLRKKSYEAAYLYYKIGQYNSAIVALQSFMNDYPEYEDIEKIDYLIIKSHKNYADQ
                     SYKLKKVERYTNEIKAFEAFKTKYPNSKYLLELEKDNKNALLNIENLKKENL"
     gene            complement(6173..6442)
                     /locus_tag="PLS16_09995"
     CDS             complement(6173..6442)
                     /locus_tag="PLS16_09995"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNJ61455.1"
                     /translation="MNFARGLLEYWSNKTKSKTYSKKLNKLFSESINLLSEHPKIGRT
                     TSNEETQISIVLDYLIFYEFNDKEIIVQSVWDARRDENKTEYDYT"
BASE COUNT         2054 a          924 c          967 g         2528 t
ORIGIN      
        1 ataagatttg cccaaattat ttcttctgta atcacttttg gtttgccagc agtaatgttc
       61 agtattttaa aaaagaaaca gtcttttggc tatggtttgg aaagtaaaag aaatattcat
      121 tggattttat ttttgataac gccactatta gttttgacta tctatccatt tttgaattta
      181 gtttttgttt taaataaaga aagtttttta ggtcagctaa tgttggaaca acaagaacaa
      241 tatcaaatgt ttattgtcgc cttgttaaag ccaaaatcaa tattttattt cctatttaat
      301 tttatactta ttgcagttat tccagcattt ttagaagaat ttttctttag aggaacttta
      361 caaagattac ttactgagag attaaatatt catttggcta ttttgataag ttcaataata
      421 ttttctttaa tacattttga gttttcagca tttttaccac gaattgtttt gggtatgctt
      481 ttaggttatg ccttttattg gtcaggaagt atttgggtag ctatttttat acacttattt
      541 aataatggat tggaagttac tttaatgtat ttaaagaatt tacatcttat atcaggaaag
      601 cttactgatg ccccacaaat gccaactcat acagaattag taggttattc tatcttattc
      661 ataatattaa gtattttgtt ctatcgtttt tctaaacgtt aaatagtgta tctttgcagt
      721 tcagttttta agaactgaaa ataaaaatca aaacgtgagt gatataagca caagaattgc
      781 cacaggtata gtctttttga ttgttatgat tggcggaacg gtttggggag aaactagtta
      841 tctagttata ttctcattag ttacaatgtt atgtattaat gaataccata atattacaaa
      901 taaagttgta caatcaatag aacataaaac cctttataaa tttatcaata ctatttttgc
      961 agccatcatt tttttgattg tttacttagt tgcgtcgaat agggtagatg ttatttattt
     1021 gtcttttatt ggtggattta ctttgtcgtg gttttttatt gaattatata ccaaatcaaa
     1081 gtcgcccttt attagtattg ctgtcaattc aatgggttta ttttatattg caattccttt
     1141 ttcagcagca agttttatag cttattatac tggagaatat aattggaatt tattattggc
     1201 ggttatgttt tttgcttggg caaacgattc ttttgcctat ttatttgggt ctaaattagg
     1261 gaaacataaa ttgtttgaaa gaatctcccc aaagaaaact tgggaaggat tttttggcgg
     1321 tattttcggt actgtagtat ttagctatat tgtttattta ttatttcctg aatggagtca
     1381 tcaagatatt gcatataaac actattttat cttagctatt ctaacatcaa tcgcttctac
     1441 ttatggtgat ttggctgaaa gtatgataaa aagaaactta gaaattaagg atacaggcaa
     1501 tactttgcca ggacatggtg gttttttaga tagatttgat ggtttaattt tttcatttcc
     1561 tgttgctact ttatatttga cctttatttc tgatttgaaa taagattctt gaataattaa
     1621 ttatatttgc ataaacataa aaatggcagt aacatatatt cataaagaag gacgaacgat
     1681 tattgcaact tcagttgtaa ttgttgcatt aattaatatc atttcaatct atgtattgaa
     1741 actgcccaat ttagttcaaa atattatttt gtcattcact ttattcgtat tgttttttat
     1801 tttctatttc ttcagaaatc caaagagaaa tatattgaca gatgataata ttattattgc
     1861 acctgctgat ggaaaagtag ttgtaatgga agaggtttat gagcctgaat attttgaaga
     1921 caaacgtttg caggtttcca tttttatgtc tccgagcaat gtacacgtaa atagagttcc
     1981 aattgctggt gaagtgaaat atgctaagta tcacgatggg aaatatttag ttgcttggca
     2041 tccaaaatca tcaacagaaa acgaaagaac ttctattgtt attgataatg gttcgattga
     2101 tgttttgatg cgtcaaattg ctggaaaagt agctcgacga attgtttatt atgtgaaaga
     2161 aggcgatatt gtagaacaag gtaaagattt tggatttata aaatttggtt caagagtgga
     2221 tttgttttta cctttagata cgaaagtaaa cgtaaaactt ggacaaaaag ttaaaggcgg
     2281 aattactatt gttggagaac tttagaatta tttccactaa attattttta tgaaaactgc
     2341 tgtaattaga agagcgacat ttaatgctgc acaccaatta tggaaccaac attggacaac
     2401 agagaaaaat aatgaaattt ttggactgtg tgcaaatccc aattttcatg gacataatta
     2461 tgttttagaa gttaaagtaa ttggcgaaat tgatttagac actggatatg taatagattt
     2521 aaaaatccta aaatctatta ttaatgattg tgttttagaa aaaatggatc atcggaatct
     2581 aaatttagat gttgcagaat ttgaacattt aaatccaacg gctgaaaata ttgcgatggt
     2641 catatacaat attatccgtg agcaattaga acctaaattt gatttgaaaa tcaaattatt
     2701 tgaaactcaa aataatattg ttgagtatcc tgcttaattt ttttctttgt tgatttgtat
     2761 ggctttcaaa cttttcctat ttcaacaaac ttttatattt ctcagcttgt gtaggatcta
     2821 agtaagaaag aatcggaact atcatacttc gttcagtcat atctgctttt gaatagatat
     2881 tttgtagttc ttcacgtttg gtttccatta ataaacgcaa agacatcaat actggattgt
     2941 ctttattgat tttttgaaat aaggatatcg atgttgtgat aatatttcta gcaacgacaa
     3001 catcatcata cattttatct agtccattaa aatgataaat taaaaaggct cttctaaaat
     3061 ctttatatct tggatttaac caagcatcta ccaacataaa tcggtttcta ttgccatcta
     3121 atttactcca acctttgtat ttacttcttt cgttagctgg aatattattg caaatagtta
     3181 atgctttttg aaagaaaact tcaccgccat tttcagcaaa agtttctcca tcataagcta
     3241 aaatcaagtt agcataaaat gctaataatg aagttaattg agaggaataa ctgttatcgt
     3301 tataatctaa aatttgcaaa tcttcatacg tgaaattaaa atccatatcc tgataagtca
     3361 gcaacatagt attgtaattg ctattaaaaa ctggtcgttg gctttgaata attgcagaag
     3421 ctttataaac accttcactt ggagaatcag aaatgtttac aatgatatta catttaattc
     3481 tttcaaaagg tttaaaagta taattactcc attttcttcc attcataaat tcttttatgg
     3541 ctttctccat attcttgaat ttaatagggt cgatatcttg attttttgga gaaattactg
     3601 aaacctcaca atttaattct tgtgcaaaag tggagcaagt aaataaaatc agcaagaaat
     3661 agagcaagaa tctttttttc ataataattc aattgcgttt aaaatatcat ttgcagtttc
     3721 gtctttactt tttaagttgt aggtctgcac ctttccagtt ttgtcaatta tagtaatttt
     3781 attggtgtca aatctaaatc cagcaccatt atcattcgtt gaatttaaaa caattaaatc
     3841 aaagtttttc ttttttattt tctgtttagc atgttcttct tcattttcag tttctaatgc
     3901 aaaaccaaca gaaatttgat gtgcttgttt tatttttcca aattcaaaag caatatctac
     3961 atttttcttt aacttaagtg ttaatgtttc atcagatttc ttaatttttt gctctgctac
     4021 atcaattggc gtgtaatcag caactgctgc agcaaaaatg caaatatatt gctgttcaaa
     4081 attttccaaa cacgcttgat acatttcttt tgccgaaagt acaggtatcg tttttatata
     4141 ttctggaata accaaattgt tttgcccaat gattaaagtt acttcagcac cacgctggtg
     4201 agctgcttta gctaaagcca aacccatttt tccactagaa tggttgccaa tgaaacgtac
     4261 agggtctaaa ggttcataag taggaccagc agtaattaat attttttgat gagctaattt
     4321 agaaggcgtt tttttttctg tgaagaatgt atttagagtt tcaataattt cttcaggttc
     4381 agccattctt cctttaccaa ttagtccact ggctaattct ccatcattaa cttctaataa
     4441 atgatgacca aaagcaacta atgtttgaat atttttttga ttgctaggat gatgccacat
     4501 atcttcgtcc attgcaggag caataaatac aggacatcta gcagaaaagt agatgcccaa
     4561 aagtaaatta tcacataagc catttgccat ctttgcaatg gtattcatag atgctggagc
     4621 aacaattatt gcatcagccc ataaggcaat atttacgtga ttgttccaat tagtttttga
     4681 agcgttggta taatctacca aacattcatt tttagaaagt gtagctaatg taagcggagc
     4741 aatgaattgg gtagctgatt cagtaataac acattgcacg tatgctcctt gcttgatgag
     4801 caatcttact aaatatgcag acttataagc tgcgatgcta ccacttacgg caacaattat
     4861 ttttttgccg cttagattca tcctactatt ttttgtcttc tacgtaagtt tcgccattta
     4921 gaaattcagc caaagccata atgcttgtct ttggtaattt ttcataataa cgagagattt
     4981 caatttgttc tctattttca aaaacttctt ctaagttatc agaaacagta gcaaattctt
     5041 ctaattttct gtgcaattct tctttcaatc ttgtatttaa ttgctcagaa cgtttagaaa
     5101 tagcattgat agatttgtat aaattgcctg ttactgcacc aattttttct aaatctcttg
     5161 tttcaatgta agcagattgc gtttttacga cttcttcttt cttattcata ttataagttt
     5221 tcttttttta aattttcaat atttaataat gcatttttat tatccttttc taattctaac
     5281 aaatatttac tatttgggta tttagtttta aaagcttcaa atgctttgat ttcattggta
     5341 tatctttcta cttttttaag tttgtaactt tggtcagcat agtttttatg ggactttata
     5401 atcaaataat cgattttctc aatatcttca tattctggat agtcattcat aaaactttgt
     5461 aaagccacaa ttgctgaatt gtattgccca attttataat ataagtatgc tgcttcgtaa
     5521 gatttctttc taagtttggc tctcaattca tccattttgt cgtttgcttc ctgcatgtat
     5581 gaacttttgg gatacttatc tataaatgct tgaaaaattg caattgcttt ttgagtatta
     5641 acttgctcta attcatatct cggcgattgt ttataatagc aaacggcttc taaaaatgca
     5701 gcttcttcag catttttact attgtaataa gtattgtaaa agcttttcaa ataaaaagcc
     5761 cccaattcgt aactgccact tagatattga gctttagcat atttaaaata aatttcttct
     5821 acactttgtt gtccttttaa aacagttaaa agtaattcgt aaataggtaa ggctttgtcg
     5881 tattttttct tttcaaaata ttcgtttgcc ttagcttttt gactttcaat atcattagat
     5941 ttagaaactt tacttggctt ttgtccaaaa gcgactaaaa aaaacaataa ggctacaaat
     6001 gtatatatta ttcttaatgg cattagccta caaagatacg atatttttta gataattttg
     6061 aatttagttt tttgaaatga atagatttta tgttaaatta ttctaatgta taaacggagt
     6121 ttaccttgtc ctaaaatagt tttaaattaa gtagggattc aaatttttaa ttttatgtat
     6181 aatcatactc agtcttattt tcatcacgcc ttgcatccca aactgattgg actattattt
     6241 ctttgtcatt aaattcatag aaaatcaaat aatctagaac aatactaatt tgtgtttctt
     6301 cattactggt tgttcttcca atttttggat gttcagataa aagatttata gattcactaa
     6361 ataatttgtt gagtttttta ctgtaagttt tggactttgt tttgttagac caatattcta
     6421 atagacctct tgcgaaattc attttagact tagattataa attcctgcaa taa
//
LOCUS       DAOQCW010000127         6418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_4951_length_6418_cov_5.948923, whole genome shotgun sequence.
ACCESSION   DAOQCW010000127 DAOQCW010000000
VERSION     DAOQCW010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6418
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4951_length_6418_cov_5.948923"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1909
                     /gene="secD"
                     /locus_tag="PLS16_10000"
     CDS             <1..1909
                     /gene="secD"
                     /locus_tag="PLS16_10000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNJ61456.1"
                     /translation="DDPNNKAKVYALYAIKMGSTDGEAPLDGAAITNSRKDIDQSGQV
                     VVSMSMNHEGARTWSDLTKRNIGNFIAIAVDNQIVSAPVVNTQIDGGNSVIQGGFTQE
                     EAQDLSNMLKIGKLPASAKIIEEELIGPSLGEASIKSGLLSLVVSIVLIFAFMMFFYS
                     AAGLIASLMMILNLFLIIGLMAGLPFGFTLTLPGIAGLVLTLGMAVDANVIIYERIKE
                     ELHHGKTALAAMQDGFKHSLAAIIDGNLTTLITGLILGMVGMGPVTGFAVTLCIGIFT
                     TLFTALLLTKVYVDFRAEQGKTYKFFTKQTEFFLKGKNIDFIGLRTKFYAFSGIIIVL
                     GFISIFVRGFDLGVDFEGGREYRVRFEQPVNVSDVRNDLNAVIGDGTIVKQIGSSTQV
                     KVTTKYMIHETSKEADDKAVQALYEGLKKYLPNVTLDNFKEVYLLSSTKVDPSVSVDF
                     RNSSIMATVLSLIAIFLYILIRFRTMGYSIGAVVATLHDALIIIALFTLLHGILPFPL
                     EVDQTFIAAILTIIGYSINDTVIIFDRLREYLKENPKSDMRTTMNAAINSTLSRTFNT
                     AVTVFIVVIILFLFGGSVLKTFSFAMLIGVFFGVYSSIFIASGIMYDYETSRFNKKKE
                     ETVAIPNRKKK"
     gene            2007..2873
                     /locus_tag="PLS16_10005"
     CDS             2007..2873
                     /locus_tag="PLS16_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HNJ61457.1"
                     /translation="MNNKVNIIECPRDAMQGIKKFIPTSLKVEYLNSLLKVGFNTLDC
                     GSFVSPQSIPQMADTFAVIPQLDLKDTDTKLSVIIANKRGAEQACEFDEITYIGYPFS
                     VSETFQQRNTNCSIEESLQRVEEIKNIADCQGKKFVAYISMAFGNPYGDLYDIAIVEH
                     WVERLIELEITRFSISDTIGCATPELMTDLFGLLKNDYPEIGFGAHFHTTPDTWQEKV
                     ETAWNAGCNRFDGAMKGFGGCPMAKDKLTGNMPTENLLAYFKKLNIDHQLDEGAFVDA
                     LHRANVIFSDYV"
     gene            2962..4158
                     /gene="trpB"
                     /locus_tag="PLS16_10010"
     CDS             2962..4158
                     /gene="trpB"
                     /locus_tag="PLS16_10010"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNJ61458.1"
                     /translation="MSLENTYTKPDEHGYYGQFGGAYIPEMLHRNIEELQEKYLDIIY
                     EDSFQKEFLQLLQDYVGRPTPLYFAKRLSEKYNTTIYLKREDLCHTGAHKINNTIGQI
                     LLAKKLGKKRIIAETGAGQHGVATATVCALMDLPCVVYMGEIDIARQAPNVKRMQLLG
                     AEVRPATSGSKTLKDATNEAMRDWIANPTDTHYIIGSVVGPHPFPDMVARFQSVISEE
                     IKKQLKAKTGKENPTKVIACVGGGSNAAGAFYHFLDQADVELFAVEAAGEGVNSGKSA
                     ATTALGKPGVLHSSRTLFMQTEDGQVVEPHSISAGLDYPGIGPIHAHLFDVGRAKFLS
                     ATDQEALDAVALLARTEGIIPALESAHALAALEKINLSKEDVVVLNLSGRGDKDLDTY
                     IKSIKF"
     gene            4228..5445
                     /locus_tag="PLS16_10015"
     CDS             4228..5445
                     /locus_tag="PLS16_10015"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HNJ61459.1"
                     /translation="MAQKLVSKPFVGAVDEHSIRVWSLFKNIDSVRFMIVDEQQNIIS
                     TSLNIFDKKIAFKQYFPIISHFENLKPNTTYQVKYILDGTEYNLLLVKTESYGNEDFS
                     FLTGSCAFVGTSFNRMVKPFNNLKILNSMEQDTAQLMLWLGDNLYYIFEKNSYRRQLK
                     RNIQVRLNKKMSAFLNSKAQYAIWDDHDFGANNSGAEFKNKKSSLNVFQQFWANPKNE
                     TLNYFTFKKQDVQFFMLDDRFYKEDSTALGVVQLTWLKESLLQSDATFKIIGIGTQAL
                     NRSGTHESFYNAKAEFDELMAFISDNKISGIFFITGDRHFAELVKMDSIIAYPLYDFT
                     TSPISMYPLKKKASEQISNPSIEGTYYNKNNYGKISIVGAAEDRKCILELKNRAGETL
                     WQYIIPAKELQFQ"
     gene            5729..5953
                     /locus_tag="PLS16_10020"
     CDS             5729..5953
                     /locus_tag="PLS16_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61460.1"
                     /translation="MKTSDIFIAHPQTNEQVSALKAFMKALNIKFEIAKSDAYKSEFV
                     AKIKNSEYQISEGNFTEVSKKDLQSFIDNL"
     gene            5950..6219
                     /locus_tag="PLS16_10025"
     CDS             5950..6219
                     /locus_tag="PLS16_10025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HNJ61461.1"
                     /translation="MIEYKIRFSAQTIEDIKEHKKSGNKAIFNKISLLLEELTLHPFS
                     GTGKPEFLKHQLTGLCSRRISKEHRLIYRVEEHIVTILSAKGHYE"
BASE COUNT         2213 a          883 c         1144 g         2178 t
ORIGIN      
        1 agatgaccca aataataaag ctaaagttta tgccttatat gcaatcaaaa tgggttctac
       61 tgacggcgaa gctccattag atggtgctgc tataacgaac tctagaaaag atatagacca
      121 atcaggacaa gttgttgtga gtatgtcaat gaaccatgaa ggagcaagaa cttggagtga
      181 tttaaccaaa agaaatattg gtaattttat tgcaattgct gttgataatc aaattgtttc
      241 agctccagtt gtgaatacac aaattgatgg tggaaattca gtaattcaag gtggattcac
      301 tcaagaagaa gcacaagatt tatcaaatat gttgaaaatt ggtaaattgc cagcatctgc
      361 taaaattatt gaagaagaat taattgggcc atcattagga gaagcctcaa ttaaatcagg
      421 tttgttatca ttagttgtaa gtattgtttt aatatttgca tttatgatgt tcttttattc
      481 tgctgctggt ttgatagcaa gtttaatgat gattttgaac ttattcttaa tcattgggct
      541 aatggcaggt ttaccttttg gatttacact tactttacca ggaatcgcag gtttagtatt
      601 gacactcggt atggcagttg atgctaacgt aattatctat gaaagaatta aagaagaatt
      661 acatcacgga aaaacagccc ttgcagcaat gcaagatggt ttcaagcatt cattagcagc
      721 aattattgat ggaaacttaa ctaccttaat tactggacta attctaggta tggtgggaat
      781 gggtcctgta actggatttg cggttacttt atgtataggt atttttacta cgctgtttac
      841 tgcattatta ttgacaaaag tttatgttga tttccgtgca gaacagggta aaacttataa
      901 attctttacg aagcaaacag aatttttctt aaaaggcaag aatatagatt ttattggttt
      961 aagaactaaa ttctatgcat tttctggtat aattatcgtt ttaggtttta tctcaatttt
     1021 tgtaagaggt tttgatttag gcgttgattt tgaaggtgga cgtgaatata gagtacgctt
     1081 tgagcaacca gtaaatgtat ctgatgttag aaatgatttg aatgcagtta ttggtgatgg
     1141 aacaattgtg aaacaaatcg gttcttctac acaagttaaa gtaactacaa aatatatgat
     1201 tcatgaaact tctaaagaag cggacgataa ggcagtacaa gctttatatg aaggattgaa
     1261 aaaatattta ccaaatgtta ctctggacaa ttttaaagaa gtttatttat tgagttcaac
     1321 aaaagtagat ccatcggtat ctgtcgattt tagaaatagt tccattatgg caactgtact
     1381 ttctctaatc gcaattttct tatatatctt aataagattt agaactatgg gttattcaat
     1441 tggagctgtt gttgcaactt tacatgatgc tttaattatt attgcattat tcactttatt
     1501 gcatggcatt ttgccattcc cattagaagt ggatcaaacc tttattgcgg caatcttgac
     1561 cattattgga tactccataa atgatactgt aattatcttc gatagattaa gagaatattt
     1621 aaaagaaaat ccaaaatcgg atatgcgaac aacgatgaat gctgcgatta atagtacttt
     1681 atcaagaaca tttaatacag cagttactgt atttattgtt gttataatat tgttcttatt
     1741 tggaggttca gttttaaaaa cattctcttt tgcaatgtta attggtgtat tctttggtgt
     1801 ttactcatca atttttattg catcaggtat tatgtacgat tatgaaacca gcagatttaa
     1861 caagaaaaaa gaagaaactg ttgctattcc aaatagaaag aaaaaataat aaaagattga
     1921 actaaatttt taaggagctt ttttaagctc cttttttatt taatatttca acttaaaatt
     1981 taaacgattt tttttaattt tataaaatga ataataaagt aaatattatt gagtgtccga
     2041 gagatgcaat gcaaggcatt aaaaagttta ttcctactag cctgaaggtt gaatacttaa
     2101 attctctttt aaaagtaggg tttaatactt tagattgcgg aagttttgtt tcgccacaat
     2161 ctattcctca aatggcagat acctttgcgg tcattcctca attagattta aaagatacag
     2221 atactaaatt atccgtgatt attgccaata aaagaggagc agaacaagct tgtgagtttg
     2281 atgaaattac atatattggt tatcctttct cggtttcaga aactttccaa caacgcaata
     2341 ccaattgttc tattgaagaa tcattgcaaa gagtagaaga gataaaaaac attgcagatt
     2401 gccaaggtaa aaagtttgtt gcttatattt caatggcatt cggaaatcct tacggcgatt
     2461 tatatgatat tgcgattgta gaacattggg tagaacgctt aattgagcta gaaattactc
     2521 gattttcaat ttctgatacc atcggctgtg caacacctga attgatgact gatttatttg
     2581 gtttattgaa aaatgattat cctgaaattg gttttggagc tcattttcat acgacacccg
     2641 atacttggca ggaaaaagta gaaaccgctt ggaatgctgg ttgcaataga tttgatggtg
     2701 caatgaaagg ttttggtggc tgcccaatgg ctaaagataa actaactgga aatatgccga
     2761 ctgaaaatct attagcttat ttcaaaaaat tgaatattga ccaccaatta gatgaaggag
     2821 cctttgttga tgccttgcat agagcaaatg ttatttttag tgattatgtt tagagcttgc
     2881 tttataatat taatttgata ctgatttaga ttggctatta gattttatta ctaacttgct
     2941 tagtttaata gatttagaat tatgagtttg gaaaatacct atacaaaacc tgatgaacac
     3001 ggatattatg gacaatttgg tggtgcctat attcctgaaa tgttacaccg taatattgaa
     3061 gaattacaag aaaagtattt agatattatc tatgaagata gttttcaaaa ggaattttta
     3121 caattattac aagattatgt cggcagacca acgcccttgt attttgccaa gcgtttaagt
     3181 gaaaagtata atactactat ctatctaaaa agagaagatt tgtgccatac aggagctcat
     3241 aaaatcaata ataccatagg tcaaatttta ttggcaaaaa aattaggtaa aaaaagaatt
     3301 attgccgaaa ctggagctgg acaacacggt gttgcaaccg caacagtttg tgctttgatg
     3361 gatttacctt gtgtggtata tatgggcgaa attgacatcg ctcgtcaagc acccaatgtg
     3421 aaacgtatgc aacttttagg agcagaagtt cgaccagcaa cgagtggaag caaaacttta
     3481 aaagatgcaa ccaatgaagc gatgcgagat tggattgcca atccaacaga tacacattat
     3541 atcattggaa gtgtggttgg accacatcct tttccagata tggtggctcg ttttcagagc
     3601 gttatttctg aagaaattaa aaaacaattg aaagctaaaa caggcaaaga aaatccaaca
     3661 aaagtaattg cttgtgtggg tggaggtagc aatgctgctg gtgcttttta tcatttttta
     3721 gaccaagcag atgttgaatt atttgcagtt gaagcagcag gcgaaggcgt aaattctgga
     3781 aaaagtgctg caacaacagc attgggaaag ccaggagttt tacacagtag ccgtacttta
     3841 tttatgcaaa ccgaagatgg tcaagtcgtt gaaccacatt ctatttctgc tggtttggat
     3901 tatccaggaa ttggtcccat tcacgcacat ttatttgatg ttggaagagc aaagttctta
     3961 tctgctaccg accaagaagc tttagatgca gttgctttgt tagcaagaac cgaaggtatt
     4021 attcctgctt tagaaagtgc acatgcatta gctgctttgg agaaaataaa tttatctaaa
     4081 gaagatgttg tcgtgcttaa tttatcagga agaggcgata aagatttgga tacatatatc
     4141 aaatctataa aattctaagc tttgcatatt tccaaacata taagcttcat agctttatta
     4201 tttatattat ttctgcccaa tattggattg gctcaaaagt tagtttcaaa accttttgtc
     4261 ggtgctgtcg atgaacattc tattcgggtt tggagtttat tcaaaaatat tgactccgtg
     4321 cgttttatga ttgttgatga acagcaaaat attataagta catcattaaa tatttttgat
     4381 aaaaaaatag catttaagca atactttcca ataataagcc attttgaaaa tttaaaaccc
     4441 aatacaactt atcaggttaa atatatttta gatggaacgg aatataattt acttctagta
     4501 aaaacggaat cttatggcaa tgaagatttt tcctttttaa cgggttcttg tgcatttgtt
     4561 ggaacctcct ttaatagaat ggtaaagcct tttaataatt taaagatttt aaattcgatg
     4621 gagcaagata ctgctcaatt gatgttgtgg ctaggagata atttatatta tatttttgaa
     4681 aaaaattcat acagaagaca actgaaacga aatattcagg ttaggttaaa taaaaaaatg
     4741 agtgcttttc taaattctaa agctcaatat gcaatttggg atgatcatga ttttggagca
     4801 aataatagtg gtgcggagtt taaaaacaaa aaatcttctc tgaatgtatt tcaacaattt
     4861 tgggcaaatc ctaaaaatga aacactaaac tattttacat ttaagaagca agatgtacag
     4921 ttttttatgt tagatgatag attttataaa gaagattcta ccgctttggg tgttgtacaa
     4981 ttgacttggt taaaggaaag cctattgcaa tcagatgcta cttttaaaat tattggaata
     5041 ggaacgcaag cattaaatag aagtggtaca cacgaaagtt tttacaatgc aaaagctgag
     5101 tttgatgaat tgatggcttt tatttctgat aataagatca gtggaatatt cttcattaca
     5161 ggagaccgtc attttgctga attagtaaaa atggatagta ttattgctta tccattgtat
     5221 gactttacca cttcgcccat tagtatgtat cctttaaaga aaaaagcaag tgaacaaatt
     5281 agcaatccaa gcatagaagg aacttattat aataaaaata actatggaaa gatttcaatt
     5341 gttggtgctg ctgaagatag aaaatgtatt cttgaattaa aaaatagagc tggcgaaacc
     5401 ctatggcaat atataattcc ggcaaaagaa cttcaatttc aataaactta gtgattagtg
     5461 aaataatgta gtaatgaaat aatgtaatta tgtagtaatg aaataattaa gtaatttctc
     5521 aatacttaat actcattact ttctacttaa attaattgtc tgaatcagga tttataggat
     5581 tttgggattg acaggatttt tataatgtag taatgaatta atttctcaat actttctact
     5641 cactactcac tactttctat ttaatactaa aaaaaccttg gctctcattt catatttatt
     5701 tagtattttt gtgttgaaat tataaaccat gaaaacatct gatattttta tagcacatcc
     5761 tcagaccaat gagcaagtat ctgcacttaa agcatttatg aaggctttaa atatcaaatt
     5821 tgaaatagcc aaatcagatg cttataaatc agaatttgtc gctaaaatca aaaatagtga
     5881 gtatcaaatt tctgagggta attttacaga agtaagtaag aaagatttac aatcttttat
     5941 tgataactta tgattgaata taagatacgt ttttctgccc aaacaataga agatatcaaa
     6001 gagcataaaa aatcagggaa taaagctatc tttaataaaa taagcttact tttagaagaa
     6061 ttaactttac atcctttctc gggaactggc aaacctgaat ttttaaaaca tcaattaaca
     6121 ggattatgct cacgtagaat tagtaaagag catagattaa tttatagagt tgaggaacat
     6181 attgttacga ttctgtctgc aaaaggacat tatgaatagt cgattacaat taagagtttt
     6241 ttgcttttag tgattagtga agtaatataa taatgtagta atgaatttct attcgtcttg
     6301 gttcttggct cttgtaatct tgggtctcat ctctcactac ttaatacttt ctacttaata
     6361 cattttttta accacaaagt acactaagtt tttacacgga gttcacaaag attaatgt
//
LOCUS       DAOQCW010000128         6317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_5079_length_6317_cov_6.083839, whole genome shotgun sequence.
ACCESSION   DAOQCW010000128 DAOQCW010000000
VERSION     DAOQCW010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6317
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5079_length_6317_cov_6.083839"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="PLS16_10030"
     CDS             <1..837
                     /locus_tag="PLS16_10030"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNJ61462.1"
                     /translation="KDKIDLSVPGSNYATRIKRDNIIDFKWGITPQFRIIDRLVLTAD
                     FSQVLIFKQQTQLNGTRDNRGPVDAVLHTYTLGLTYNFGKKQHIDWAKKELATKKALD
                     DLKNEYYKDREGIKGEIQSLRDEVNALNAKLNNAATNEDLDKLRNELNQTINDKLKSI
                     QNSTEKGETILDLINSGSLATYFDFDVRTPQATSMQGIQQIADYLKANPNAKLTVTGY
                     ADAIGTKAYNNQLAQDRANNIKNILVQQGIDASRINAVSGGIDNSTNRNLARKASYNI
                     SK"
     gene            complement(890..2023)
                     /locus_tag="PLS16_10035"
     CDS             complement(890..2023)
                     /locus_tag="PLS16_10035"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HNJ61463.1"
                     /translation="MIFYRIFFICLFFIKLTNLFAKEKIIPNHNFGIDASYQIGKVFK
                     HTKNFQPTITGATNVFQISAFKQTNGKKDWQRKLKYPELGGGLIALLHQNKDTIGNGY
                     AAFAYFTYPFVRSRIVDFILRFSGGVAYDSNIYDEQNNPVNNAMSSPINIFVEARLGL
                     NFKIYKNVELLTGIAFAHYSSGGTRLPNLGLNTPTFLLGLRYIGFSKKSMPYNTELVE
                     KPKYKNEMGFLLNNGWSDITKFDKDKCHIITSGSVQYARYFNIVNKLYCGALLEFDFG
                     QPHLHENRALVQNKLIKKAASRMSIYVGNEIAFGRTSIFYALGAYLYTPYKMFTPIYF
                     KIGTNINFLALSKKNRPYFNFGVKAHAGIASYTEIGLGSNFKF"
     gene            complement(2025..3308)
                     /locus_tag="PLS16_10040"
     CDS             complement(2025..3308)
                     /locus_tag="PLS16_10040"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNJ61464.1"
                     /translation="MLIVEYFVYVLYFSFMQLDIQIPNIVEELQNKLKANQEQLHFLL
                     NITEAINMNCSTDQLIELYQEVIQEQLGVGEIAFFVFNGVWKRKIWLKEEEQDTFIPI
                     ADNIHKYFTPKILDEEEQLLFHNFKFLIPVYHKSQAIAFVLFGNMNSNSFFNETELLE
                     FAQVITNIIAVALENKRLFKQEIEKKEYEKELELASKVQGMLIPKKLPKNNLYEFDGI
                     YVPYKGIGGDYYDVIHINKNEFVFCVADISGKGISAALVMANIQAYLNAVLDMQMSLE
                     TLIHKLNKKIFTVTEGNMFVTLFLAKYNIVSKEMTYINCGHVPPILINPHQMQLLEKG
                     TTMLGVFEDLPKMSSETITIKEATSIVCYTDGLTELENDNNKQFGINLLLDFLKFNYQ
                     FNPALFNKLLHEHISKYKGNSLFNDDISILTGKFY"
     gene            3391..5046
                     /locus_tag="PLS16_10045"
     CDS             3391..5046
                     /locus_tag="PLS16_10045"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNJ61465.1"
                     /translation="MEKYESPLERFYHWEKTQPNKTFFRQAYQGQWLSVTWAQAGTEI
                     RKMANYLKSLNLPEHSKIAIISKNSMHWIMSDLAIMMSGHISVPLYPTIPGDLIKYCL
                     EHSETKVCFVGKLDNWKTQAHGIPDGVKVINFPMWSEYYDGYINWEDIMKNTEPLMEN
                     PIPNPDDLITIIYTSGTTGLPKGVLHRYGAIGFAAANAMKLLDNLSPADRFFSYLPLS
                     HIAERMLVEMGSIYSGGTMSFAESLETFPMNLKETKPTVFLAVPRIWTKFQMGILAKM
                     NQNKLDLLMRIPIVNNLIKKKIKEGLGLENAKYLLSGAAPLPVATTNWFDHIGFRIYE
                     AYAMTENCAYSHLSLPNSRKVGSVGCSLPFNEVRFSEEGEIQVKSDAIMVGYYKEPEK
                     TRETITEDGWLCTGDQGVEDNKGFLTITGRVKDIFKTDKGKYVAPAPIENELSKSTLI
                     EQVCVVGANLPQTMALVVLSEEAKGKNKNEVDKELEELATSVNKGLEKHERMKKVVVM
                     NEEWTVENNLLTPTLKVKRNILEKQKQDAYQNWYQQKESVVWE"
     gene            complement(5076..6173)
                     /locus_tag="PLS16_10050"
     CDS             complement(5076..6173)
                     /locus_tag="PLS16_10050"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNJ61466.1"
                     /translation="MKFLHLIISFFSRTINIIGVVGLLVCLSASYISPENFGLIAVLG
                     FVFPIFVALNLLFVLIWILRKRWFFMVSLLALLLSAAQIFQLIGFRKSQIISEPNLKI
                     MSYNVRNFDLYNWSENKQAQDKIFKTIEEENPDIICFQEFYSDTSENFNTIKRLKKLG
                     YKHYFFVRELVLRNTDEWGIATFSKLPILSSNKILKEDESNSYKRFPYKGISTDVSFQ
                     DKQIRIFNVHLQSIHFGKNEYQVIEDLKGKEIKENAVKKMLRKLRNAFQKRAVQTRIF
                     QSILNEQNQPYVICGDLNDLPNSYTYNILAKNMNDAFLQNNIGIGQTFNRIPLLRIDY
                     ILTTKDFKTSDCRVIKNPISDHYPIVTKVGF"
BASE COUNT         2311 a          951 c          939 g         2116 t
ORIGIN      
        1 aaagataaaa ttgacttgtc agtaccagga agcaattatg ctacaagaat taaaagagac
       61 aatattattg attttaaatg gggtattaca ccacaattta gaattattga tagactagta
      121 ttgactgctg attttagcca agtattaatc tttaaacaac aaactcaatt aaatggaaca
      181 agagacaaca gaggtcctgt tgatgctgtt ttacatactt atactttagg tttgacttat
      241 aactttggca aaaaacaaca catcgattgg gctaaaaaag aattagcaac gaagaaagct
      301 ttagatgatt tgaaaaacga atactataaa gacagagaag gtataaaagg tgaaattcaa
      361 agtttaagag atgaagttaa tgctttaaat gctaagttaa acaatgctgc aacaaacgaa
      421 gatttggata aattaagaaa tgaattaaac caaactatca acgacaagtt aaaatctatt
      481 caaaattcaa ctgaaaaagg agaaactatt ttagatttaa ttaatagtgg atcattagct
      541 acttattttg attttgatgt aagaacacca caagctacat caatgcaagg tatccaacaa
      601 attgctgatt atttaaaagc taaccctaac gcaaaattaa ctgtaactgg ttatgctgat
      661 gcaattggaa caaaagctta caacaatcaa ttagcacaag atagagctaa caatattaaa
      721 aatatattag tacaacaagg tatcgatgca tcaagaatta atgcagtatc tggtggaatt
      781 gacaattcaa ctaatagaaa tttagcgaga aaagctagct acaatatttc taaataatag
      841 cttattgctt taaatgatga gtccgccttt tggcggactt tttttatgcc taaaatttaa
      901 aattacttcc taagcctatt tcagtataag atgcgatacc agcatgtgct ttaaccccaa
      961 aattaaaata tggtctgttc ttcttgctta aagctaaaaa attaatatta gtaccaattt
     1021 taaaatatat aggtgtaaac attttataag gtgtgtataa ataagctcct aatgcataaa
     1081 aaatagacgt tcgtccaaat gcaatttcat taccaacata tatactcatt ctgcttgctg
     1141 cttttttgat taatttattt tgaaccaatg ctctgttttc atgtaaatgt ggttgtccaa
     1201 aatcaaattc taataatgca ccacaatata atttatttac gatattaaaa taacgggcat
     1261 attgtaccga acccgatgtt ataatatgac atttgtcctt atcaaatttt gtaatatcac
     1321 tccaaccatt atttaacaaa aagcccattt catttttata ttttggtttt tcaacgagtt
     1381 cagtattgta tggcattgac tttttagaaa agccaatata ccttaagcca agtaaaaaag
     1441 ttggcgtatt aagtccaaga ttgggcaatc ttgtgccacc actggaataa tgtgcaaatg
     1501 ctatgcctgt gagtaattca acattcttat aaatcttaaa gttcaaccct aatcgagctt
     1561 ctacaaatat attgataggt gatgacattg cattattgac tggattattt tgctcgtcat
     1621 aaatattaga atcataggca actccaccag aaaatcttaa aataaaatca acaattctac
     1681 ttctaacaaa tgggtatgta aaataagcaa aggctgcata gccattgcca atagtgtctt
     1741 tattttgatg taataaagca ataagacctc cgcctaattc tggatatttt agttttcttt
     1801 gccaatcctt tttgccattc gtttgcttaa aagctgaaat ctgaaaaaca ttcgttgctc
     1861 cagtaattgt tggttgaaaa tttttagtat gtttaaaaac ctttccaatt tgataactag
     1921 catctatccc aaaattatga tttggaataa ttttctcttt tgcaaaaaga ttcgtaagtt
     1981 ttataaaaaa aagacaaata aaaaatattc tataaaaaat cattctaata gaattttcct
     2041 gtcaatatac taatatcatc attaaataag gagttgcctt tatattttga aatatgctca
     2101 tgcaataact tattaaacaa tgcaggatta aattgataat tgaactttaa aaaatctagg
     2161 agaagattaa ttccaaactg cttgttatta tcattttcta attcagttaa accatcagta
     2221 taacaaacaa tactggtggc ttcttttata gtaattgttt cagaactcat ttttggcaaa
     2281 tcttcaaaaa cgcctaacat ggttgttcct ttttctaaca attgcatttg atgaggatta
     2341 atcaatatcg gaggtacatg cccacaattt atatacgtca tctccttact tacaatattg
     2401 tattttgcta aaaacaaggt aacaaacatg tttccttctg ttactgtaaa tatcttctta
     2461 tttaatttat gaattagcgt ttctaaagac atttgcatat ccaaaacagc attcaaataa
     2521 gcctgaatat ttgccattac taatgccgct gaaattcctt tgccagaaat atccgcaaca
     2581 cagaatacaa attcattttt gttgatatgg ataacatcat aataatcgcc tccaatacct
     2641 ttgtatggta cgtagatccc atcaaattca tataagttat ttttgggtaa ctttttagga
     2701 attagcatac cttgtacttt tgaagctaat tctaattcct tttcatattc ttttttctca
     2761 atttcttgct tgaaaagtct tttgttttct agggcaactg caataatatt ggtaataact
     2821 tgagcaaatt ccagcaattc tgtttcatta aaaaaactat tcgaattcat atttccaaat
     2881 agtacaaatg caatggcttg agatttatga taaactggga ttaaaaattt gaagttgtga
     2941 aaaagtagtt gttcttcttc atctaaaatt ttgggtgtaa agtatttatg aatattatca
     3001 gcaataggaa taaaagtatc ttgttcttct tcttttaacc aaatttttct tttccatacg
     3061 ccattaaaga caaagaatgc aatttctcca acacctaatt gttcctgaat aacttcttga
     3121 tagagctcta ttaattgatc cgtagaacaa ttcatattaa tagcttcggt aatatttaac
     3181 aaaaaatgca attgctcttg attcgctttt aatttatttt gaagctcctc cactatgttt
     3241 ggtatttgaa tatccaattg cataaaacta aaatacaaaa cataaacgaa atattctact
     3301 ataagcaaaa attgaatttt taatttttat caaaaaaact tgtatttaca aaagatttcc
     3361 ttatttttaa aataatataa acaaaaacga atggaaaaat atgaatcgcc tttagaacga
     3421 ttttatcatt gggaaaaaac acaaccaaat aagacttttt ttagacaagc ttatcaaggt
     3481 caatggttgt ccgttacttg ggcacaagct ggtactgaaa ttagaaaaat ggctaattat
     3541 ttaaagtctt taaacttgcc tgagcattca aagattgcaa ttatatctaa aaatagtatg
     3601 cattggatta tgagtgattt ggcaattatg atgtctggtc atatttctgt tccattgtat
     3661 cccacaatac ccggcgattt gataaaatat tgtttagaac atagcgaaac taaggtttgt
     3721 tttgtaggaa aattagataa ttggaaaact caagcacacg gcattcctga tggcgtaaag
     3781 gttataaact ttccgatgtg gtcagaatat tacgatggct acatcaattg ggaagatatt
     3841 atgaaaaata ctgaaccatt aatggaaaat cctattccaa atccagatga tttaattaca
     3901 attatttata catcaggcac aacaggtttg ccaaaaggcg ttttacacag atatggagca
     3961 ataggttttg cagctgccaa tgccatgaag ttactagata atttaagtcc tgcagataga
     4021 tttttttcct atctaccatt atcgcatatt gcagaaagaa tgttagtgga aatgggtagt
     4081 atatattctg gcgggacgat gtcatttgct gaaagcttag aaacttttcc aatgaatttg
     4141 aaggaaacga aacctactgt tttcttagct gttccgagaa tttggactaa gttccaaatg
     4201 ggtattttag caaaaatgaa tcaaaataaa ttggatttgc tgatgcgtat tccaattgta
     4261 aataatttga ttaagaaaaa aattaaagaa ggtttgggct tggaaaatgc caaatattta
     4321 ttgagtggtg ctgcaccact tcctgttgcg acaaccaatt ggtttgatca tattggattt
     4381 agaatctatg aagcttatgc gatgaccgaa aactgtgcct attctcattt aagtttgcct
     4441 aattcaagga aagttggtag tgtgggttgt tcattacctt ttaatgaagt tcgtttttca
     4501 gaagaaggag aaatacaagt aaaaagtgat gcaataatgg ttggctatta caaagaacct
     4561 gaaaaaacca gagaaaccat taccgaagac ggttggttat gtactggcga ccaaggtgtt
     4621 gaagacaata aaggattctt aacaataaca ggaagagtta aagatatttt caaaactgac
     4681 aaaggaaaat atgtagctcc agcaccgatt gaaaatgaac tgtcaaaatc tactttaata
     4741 gaacaagttt gtgtggttgg agcaaatttg cctcaaacaa tggcattggt tgtgctttca
     4801 gaagaagcaa aaggcaaaaa caaaaatgag gtggataaag aattagaaga attagcaact
     4861 tccgtcaata aaggattaga aaaacatgag cgaatgaaaa aagtagtggt tatgaacgaa
     4921 gaatggacgg ttgaaaataa cttactaaca ccaacattaa aagtaaaacg taacatcttg
     4981 gagaaacaaa aacaagatgc ttatcaaaat tggtatcaac aaaaagaaag cgtggtttgg
     5041 gaataaaata aatagaaatt aaactataac aatttttaga atccaacttt ggttacaatt
     5101 ggatagtggt cagaaattgg atttttaatc actctacaat cacttgtttt aaaatctttt
     5161 gttgtcaaaa tgtaatcaat tctcaacaaa ggaatacggt taaaagtttg gccaatgcct
     5221 atattatttt gtaaaaaagc atcattcata ttcttagcaa gtatgttata cgtataggaa
     5281 ttcggcaaat cattcaaatc gccacaaatg acatacggtt gattttgttc atttaaaatt
     5341 gattgaaaaa tccttgtttg tacagctcgt ttttgaaaag catttcttag ttttctaagc
     5401 atctttttta ctgcattttc ctttatttcc tttcctttta aatcttcaat aacttgatat
     5461 tcattttttc caaaatggat agattgaaga tgaacattaa aaattctaat ttgcttatct
     5521 tgaaagctaa catccgttga tattcctttg tagggaaatc ttttataact attgctctca
     5581 tcttctttca atattttatt agaagaaaga atgggtaatt ttgaaaaagt agcaataccc
     5641 cattcatctg tatttcgcaa aaccaattct ctgacaaaaa aataatgctt atatcctaat
     5701 tttttcaatc gttttatcgt attaaaattc tcagacgtat ctgaataaaa ctcttgaaaa
     5761 caaataatat caggattttc ttcctctatg gttttgaaaa ttttatcttg tgcttgttta
     5821 ttctcactcc aattatacaa gtcaaaattt cgaacattat aagacatgat ttttaaattc
     5881 ggctcactaa ttatttggct tttacgaaac ccaataagct gaaaaatttg agctgcactt
     5941 aaaagcaatg ctaaaagaga aaccataaaa aaccatcgct ttctaagtat ccaaatgagt
     6001 acaaataata gatttaatgc aacaaaaatc ggaaatacaa aacctaatac ggctatcaat
     6061 ccaaaatttt caggacttat ataacttgca gataaacaga ccaacaagcc tacaacacca
     6121 atgatattga tagttcggga gaaaaatgat ataatcaaat gtaagaattt caatcttctt
     6181 tacttatttt gaatttgttt attgctgaat taaatccatt attaaagaat cgtgtgtcat
     6241 taataaaaca aaaattacaa ctatcattga tttttaaata ttttgaatta aagtaattat
     6301 aatatttcaa tattttt
//
LOCUS       DAOQCW010000129         6255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_5151_length_6255_cov_5.687097, whole genome shotgun sequence.
ACCESSION   DAOQCW010000129 DAOQCW010000000
VERSION     DAOQCW010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5151_length_6255_cov_5.687097"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..1454)
                     /locus_tag="PLS16_10055"
     CDS             complement(186..1454)
                     /locus_tag="PLS16_10055"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61467.1"
                     /translation="MDSVSYSIRYNPNKSTFPIGDNVPWEKAAIFIEQKEFILDPDWH
                     AGAYYVQESNSMFLGYILEQIQVQKSSQIVLDVCAAPGGKSTHILDKINKDSILISNE
                     ILPKRNNILRENLIRWGNPNIIVTQNDTKDFKSLNNYFDLVVLDAPCSGEGLLRKKPE
                     LAVEFTPENIEKCAIRQKEIIDNIKNTVKQNGYLVYSTCTFQTCENEDQVQYLLDNGF
                     EIIPIDITLFPEITIGKIPHTYYFSFEKTKGSGFFIACLKKVDTTQNIPSNFYDYSRK
                     NQISEVKFNEAEKWIKNSATFKYFTFKNNIFSFPKIFDSVLNIIQTHLYITYFGIEIG
                     ELKKDIFIPAHNLALSNYLQTDLPTIEINKPTALQFLRKQNIEKETINSLNFSGWVLL
                     SYNQHCLGWVKILQDRINNYLPNNWRILKS"
     gene            complement(1485..2915)
                     /locus_tag="PLS16_10060"
     CDS             complement(1485..2915)
                     /locus_tag="PLS16_10060"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HNJ61468.1"
                     /translation="MKKILSALLFIIVVYNINAQAFLGAHYTTKDAILTNSLNPAMPA
                     ASSMKWQVNLFGININAGNNYLGIQSFKGIASNFDKDVNIGEILDGKSKDAHVGLGFN
                     GPAFMIKIKKNNAISIGVRGRGVATINDLNEDLVQSMYHNFKDIFEWAPHFKDDKLTG
                     GANVYHEIYAGYSRTINIKARHALHVGVTGKMITNIFNAQVEAHNVDFNIVHSGMDSF
                     VNVKNSQFDFLVSDRMDDGFKYKFGIDGVGFDVGLIYELKRKETDEPLLLAGFSVNDI
                     GFNSYNLGKNSRTFIGNNTNIPASHILNADGSTIEFDMLLDSLGTKTIPTGKKKMKLP
                     MTLNTFVDVRIAKMFYANANFQFNPYDFKKGEPKANMPTNITITPRFETRIFSAYLPV
                     TWNKYSGFNAGLAFRIGQVTLGSSNVITSFIKKPTSKLDFYMNISFGKMAKAGKIKED
                     KPKKDKSSNNKTEENRDDTPSRETTN"
     gene            complement(2978..3475)
                     /locus_tag="PLS16_10065"
     CDS             complement(2978..3475)
                     /locus_tag="PLS16_10065"
                     /inference="COORDINATES: protein motif:HMM:NF012479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNJ61469.1"
                     /translation="MKNSISTFKVKKANQKEINLSDYQGKVLMIVNTASKCGLTPQFE
                     QLEQLYQEFKDQGFEILGFPSNDFAGQEPNSAEGAEQFCQLNYGVSFPIMEKIHVKKG
                     ANQHELFKFLGNNAPGPNLLTHPKWNFQKYLINKNGEVIDYYIPTTEPTSEKVKDKIR
                     ALLKS"
     gene            complement(3411..4070)
                     /locus_tag="PLS16_10070"
     CDS             complement(3411..4070)
                     /locus_tag="PLS16_10070"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HNJ61470.1"
                     /translation="MKQLISFLSFTLLCFCVKAQKMDTIILPSIVIGGDTLVEEVILE
                     PIIVAASKLKPVTISYRDQTYLKKVYPYALRIARLSQVIDEQLKNMSKKERQKFLNNA
                     ENLLRDAYEKELKNMTRTQGKFLIKLVYRETGVTVFDLLKEYRGGFKTFWWNFGSKFF
                     DLNLKATYDPKGTDAELENYVIRLDETYQRNGTKYIIQNEKFNLNIQSKKGKSKRDKS
                     E"
     gene            complement(4141..5109)
                     /locus_tag="PLS16_10075"
     CDS             complement(4141..5109)
                     /locus_tag="PLS16_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNJ61471.1"
                     /translation="MHSIEELRTFFLDYLNQNKFIQTPSELYEPNNYFLQIGGKRLRP
                     TLLLLCTELFGGKYENALDAALATEYFHNFTLIHDDIMDNAPLRRGFPTVHEKYNLTT
                     GILSGDVLMIQAYQCLSNIDKKYFKQIFDIFTKTAIEVCEGQQMDINFEQHVNVQHQD
                     YLEMIKLKTAVLLAASMQIGAIIGGANEKDADTIYQYGLNLGIAFQIQDDILDCFGKP
                     EQVGKQLGGDILQNKKTLLLLEAIKIAEIRNDNQLKKALTIEHSKEKLKTILAIFNTY
                     QIKEYGTKIRDSYIEQSIAYLNKINIDKEKKAILNKLIEHLVLRSF"
     gene            5214..5867
                     /locus_tag="PLS16_10080"
     CDS             5214..5867
                     /locus_tag="PLS16_10080"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNJ61472.1"
                     /translation="MQDNSKVIDELIDKRRSVRIYDDSEPLNDVVVRKCLERATLAPS
                     SDNLQLWEFYRVKSKNKLQELAKYCYGQKAASTAQELVVFVTRTDKWQERAEFNLEEI
                     KKGFPKEITNKEQKVIDYYEKSIAQLYSGNEAKAEIRIIAHKSCALAAQMFMLSIAAE
                     GFDTCPMEGFQEELVKQFLNLPDIAEICMVISVGKAKPEGIYNRRIRVPNDAVIFEI"
     gene            complement(5879..>6255)
                     /locus_tag="PLS16_10085"
     CDS             complement(5879..>6255)
                     /locus_tag="PLS16_10085"
                     /inference="COORDINATES: protein motif:HMM:NF017551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HNJ61473.1"
                     /translation="SNTIQSVSNGIHGLSNALLDTPWKKVDRIKSDLENAIQNNHLEK
                     DFLFDLLCNEDKANPEDLPSTGLSLELEKAMSSIFIHTPFYGTRCSTIILKDKNGKVS
                     FTERKYSENKEILGEVNYEIES"
BASE COUNT         2061 a          915 c          910 g         2369 t
ORIGIN      
        1 gaaaatagtt tttattctgc caatgttgat accatttttg ttcaatttct gatggtttga
       61 tatttttagc taattccata ttttttagta ctaatacaat tttttaaaga atcacgaaga
      121 tacgaaacaa tgaaataatt caataattca acaatgaaat aattcaacaa tgaaataggt
      181 agtaattaac tttttaaaat tcgccaatta tttggtaagt aattattgat tctgtcttgt
      241 aagattttta cccaacctaa acaatgttga ttgtacgata ataaaaccca gcctgaaaaa
      301 tttaaactgt tgattgtctc tttttcaatg ttttgctttc ttaaaaattg caaggcagta
      361 ggtttattaa tttctattgt aggcaaatca gtctgtaaat aatttgacaa tgccaaattg
      421 tgtgctggta taaaaatatc tttttttaat tcgccaattt caataccaaa ataagtaata
      481 tatagatgtg tttgtataat atttaatact gaatcaaaga ttttaggaaa tgaaaatata
      541 ttatttttga aggtaaagta tttaaaagtc gctgagtttt taatccattt ttcagcctca
      601 ttgaatttta cttcggagat ttgatttttt ctactataat catagaaatt actaggtata
      661 ttttgtgttg tatctacttt ttttaaacag gcaatgaaaa aacctgaacc tttagttttt
      721 tcaaaagaaa aataataagt gtgtggtatt ttgccaattg taatttctgg aaataaagta
      781 atgtctattg gtatgatttc aaagccatta tctaataaat attgtacttg gtcttcattt
      841 tcgcaagttt gaaaagtgca agtagaataa actaaatagc cattttgttt taccgtattt
      901 ttaatattat caatgatttc cttttgacgg attgcacact tttcaatgtt ttctggagta
      961 aattctactg ctaattcggg tttctttctt aataagcctt cgccactaca tggtgcatct
     1021 aatacaacta aatcaaaata attatttaga cttttaaaat ccttcgtgtc gttttgtgtt
     1081 acaataatat ttgggtttcc ccaacgtatt aaattctctc ttaaaatgtt gttgcgtttg
     1141 ggtaatattt catttgaaat taaaatcgaa tccttattaa ttttatccaa aatatgtgtt
     1201 gattttccac ctggtgccgc acaaacatct aaaactattt gactactttt ctgaacttga
     1261 atttgttcta aaatataacc taaaaacata gaattgcttt cttgaacata atatgcacca
     1321 gcatgccaat ctggatcaag aataaattct ttttgttcta taaaaattgc agccttttcc
     1381 caaggaacat tatctccaat tggaaaagta gatttatttg ggttataacg aatactataa
     1441 gaaacagaat ccaaaattat attttggaca aaaataatga ttttctaatt agttgtttca
     1501 cgagatggcg tatcatctct gttttcttca gttttgttat tggatgattt gtccttcttt
     1561 ggtttatctt ctttgatttt accagcttta gccattttgc caaaactaat attcatatag
     1621 aaatctaatt tgctcgtagg ttttttgatg aatgaagtaa tgacattact tgaacctaag
     1681 gttacttgac caattctaaa agctaatcca gcattaaatc cagaatattt attccaagtt
     1741 acaggtaaat aagcggagaa gattctagtt tcaaatcttg gcgtaatagt aatgtttgta
     1801 ggcatatttg cttttggttc ccctttttta aagtcataag gattaaattg aaaatttgca
     1861 ttggcataaa acatctttgc gattctgaca tctacgaaag tgtttaaggt cattggtaat
     1921 ttcattttct ttttaccagt tggaattgtt ttagtaccta atgagtctaa taacatatca
     1981 aattcaattg tgcttccatc agcatttaaa atatgacttg caggaatatt agtattatta
     2041 ccgataaatg ttcgactatt tttacctaaa ttataagaat taaatccaat atcattaaca
     2101 gagaatccag ctaataatag aggttcatct gtttcttttc gttttaattc ataaattaaa
     2161 ccaacatcga atccaacacc atcaatgcca aatttatatt tgaaaccatc atccattctg
     2221 tcgcttacta aaaagtcaaa ttgtgaattt tttacattta caaaactatc cattccagaa
     2281 tgaacgatat tgaaatcaac attatgagct tcaacttgag cattgaatat attagtaatc
     2341 atttttccag ttacgccaac atgtaatgcg tgtcttgctt taatattgat agttcttgaa
     2401 taacctgcat aaatttcatg ataaacgttt gctccacctg tgagtttgtc atctttaaaa
     2461 tgtggagccc attcaaaaat atctttgaaa ttatggtaca tagattgtac caaatcttca
     2521 tttaagtcat taatagttgc aacgccacgg cctctaacac ctattgatat tgcattgttt
     2581 ttctttattt taatcataaa agcaggtccg ttaaatccta agccaacgtg agcgtctttg
     2641 gattttccat caagtatttc tccaatattt acatctttat caaaatttga ggcaatacct
     2701 ttaaaagatt ggattcctag gtaattattt cctgcattaa tattaattcc aaatagattt
     2761 acttgccatt tcatactaga tgcggcaggc attgcagggt ttaggctatt ggttaagatg
     2821 gcatcttttg tggtatagtg ggcacctaaa aatgcttgag cattaatatt atagacaaca
     2881 ataataaata gaagtgccga aagtattttt ttcataatat ttaatttttg ttaaatatat
     2941 taagaaattt gaactcacgc aactattttt tttctattta cgattttaat aatgctcgaa
     3001 ttttatcttt tactttttcc gaagtgggtt ctgttgttgg aatatagtag tcaatgactt
     3061 ctccattttt attgattaaa tatttttgga aattccattt aggatgtgtt aataaatttg
     3121 gaccaggagc gttattacct aaaaacttaa atagttcatg ttgatttgct ccttttttta
     3181 catgaatctt ttccataatt ggaaagctaa cgccataatt tagttggcaa aattgctcag
     3241 caccttcagc agaattaggt tcttgtcctg caaaatcatt agaaggaaaa cctaagattt
     3301 caaaaccttg gtctttgaat tcttggtaca attgttctaa ttgctcaaat tgaggtgtta
     3361 gtccacattt ggaagcagta tttacaatca ttaatacttt tccttggtaa tcactcagat
     3421 ttatctcttt ttgatttgcc ttttttactt tgaatgttga gattgaattt ttcattttgt
     3481 ataatatatt ttgtgccgtt tctttgataa gtttcatcta atcgaatgac atagttttcc
     3541 aactcagcat ctgtaccttt gggatcataa gttgctttta aatttaagtc aaaaaattta
     3601 gaaccaaaat tccaccaaaa agttttaaat ccaccacgat attcttttaa taaatcaaat
     3661 acagttacac cagtttcacg atagactaat ttaattagaa atttaccttg cgttctcgtc
     3721 atatttttga gctctttttc gtaggcatct ctcaataaat tttcagcatt atttaaaaat
     3781 ttttgtcgct cttttttaga catgtttttt aattgctcat caataacttg actaagtcta
     3841 gcgattctta aagcataagg ataaactttc tttaaatagg tttggtctcg atatgaaatt
     3901 gtaacaggtt ttaatttaga agcggcaact atgattggtt ctaatataac ttcttctacc
     3961 aaagtgtctc caccaataac aattgaagga agaattatgg tatccatttt ttgagcctta
     4021 acacaaaaac atagaagcgt gaaagataga aatgagatta gttgtttcat aatttattgt
     4081 attccaaaat tatgccataa aatttaatgt aaaataatgt tttaaaataa aaaatgttaa
     4141 ttaaaaactt cgcaaaacta aatgttcaat cagcttatta agaatggctt ttttctcctt
     4201 atcgatattg attttattga ggtaagcaat agactgttct atgtaggaat ctctaatttt
     4261 tgttccgtat tctttaattt gataggtatt gaaaatagct aatatcgttt taagtttttc
     4321 tttggaatgt tctattgtta aagctttttt taattgattg tcatttctaa tttctgcaat
     4381 tttaatagct tctaatagta ataaagtttt tttattctga agaatgtcgc caccaagttg
     4441 tttaccgact tgttcaggct ttccaaagca atctaaaata tcatcttgaa tttgaaaagc
     4501 aataccaaga tttaaaccat attgataaat agtatctgca tctttctcat ttgcacctcc
     4561 aatgatagca ccaatttgca tacttgcagc cagcaaaact gctgttttca gtttaatcat
     4621 ttccaaataa tcttgatgtt gtacattcac atgttgctca aaattgatat ccatttgttg
     4681 tccttcacaa acttcaattg ctgtttttgt aaatatatca aatatctgtt tgaaatactt
     4741 tttatcaata tttgacagac attgatatgc ttgaatcatt aatacgtcgc cacttaaaat
     4801 accagtagtt aagttatatt tttcatgtac cgttggaaaa ccacgtcgca aaggagcatt
     4861 gtccataatg tcatcatgaa ttaaagtaaa attgtgaaaa tattctgttg ctaaagcggc
     4921 atccaatgca ttttcgtatt tgccaccaaa taattctgtg cataaaagta ggagtgtagg
     4981 tcgtaatctt ttacctccaa tttgtaagaa ataattatta ggttcatata attcactagg
     5041 tgtttgaatg aatttatttt gattgagata gtctaaaaaa aaagtcctta attcttcaat
     5101 tgaatgcata gccaaaaata catttttaat tttgaattct aagagcttgt tacttattta
     5161 tgaatttaat tatattcgtt aaagttctcc ataaaaatta ttatcattgc aatatgcaag
     5221 ataattctaa agtcattgat gagcttattg ataaacgacg ctctgtaagg atttatgatg
     5281 atagtgaacc attaaatgat gttgttgtaa gaaaatgttt ggaaagggcg actttagcac
     5341 caagctccga taatttacaa ttgtgggaat tttatagggt aaaatctaaa aataaattac
     5401 aagaactggc aaaatattgc tatggacaaa aggctgcatc aacagctcag gaattggtgg
     5461 tttttgtaac aagaacagat aaatggcagg aacgtgcaga atttaattta gaggaaatca
     5521 aaaaaggttt tcctaaagaa attacgaata aagagcaaaa agtaattgat tactatgaaa
     5581 aatcaattgc tcaattgtat tctggtaacg aagctaaagc tgaaattaga attattgctc
     5641 ataaaagctg tgcattagct gctcaaatgt ttatgttgtc tattgcagcc gaaggctttg
     5701 atacatgccc gatggaaggt tttcaagaag aattagtaaa gcaattttta aatttaccag
     5761 atattgctga aatttgtatg gtaatttcag taggaaaagc caagccagag gggatttata
     5821 atagaagaat tcgagttcca aatgatgctg ttatttttga aatatagttc agtataattt
     5881 acgattctat ttcataattt acttcaccta atatttcctt attttctgaa tattttcgtt
     5941 ctgtaaaaga tacttttcca tttttatctt ttaaaataat tgtagaacat cgtgtgccat
     6001 aaaatggtgt atggataaaa attgaagaca tcgctttttc tagttctaat gataaacctg
     6061 ttgatggtaa gtcttcagga tttgctttgt cttcattgca taataaatca aataaaaaat
     6121 ctttttctaa atgattattt tgaatggcat tttctaaatc gcttttgatt ctatctactt
     6181 ttttccaagg cgtatctaat aaagcattac tcaatccatg aataccattg gaaactgact
     6241 gaatggtatt ggaaa
//
LOCUS       DAOQCW010000130         6180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_5257_length_6180_cov_5.709551, whole genome shotgun sequence.
ACCESSION   DAOQCW010000130 DAOQCW010000000
VERSION     DAOQCW010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6180
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5257_length_6180_cov_5.709551"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..1278)
                     /locus_tag="PLS16_10090"
     CDS             complement(205..1278)
                     /locus_tag="PLS16_10090"
                     /inference="COORDINATES: protein motif:HMM:NF024783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNJ61474.1"
                     /translation="MSKQNYEEYWKLTNAFTDYNGKKFLDTLAVCIKFVDEYREESYS
                     DEKYLRLQLEVEKVNPINLISIRKSINQLVKMGFINSFLLSYHPQAKEYIEARTNKKR
                     ETLLSKIIYSNSSFNRAVNIDSPIKQINFLIQTLIEKGKLSKEEIIALMLVDIEAHKR
                     TFISESELQKYVEEATKIGFIKRKYNQVGYLYNILGKLDDLVFVKDDLYFKEDAQQIF
                     GEDLSAISKKRDPYLHRLYKNQLQEECEEIYGKPLCVLEKLAYPVLIASHIKPFVQSD
                     ETEAYDPNNGLLLSRTVDSLFDLKYISFTDEGKMLFSKRISEDVKEFWKNYTLEPSIL
                     NEKRKSYLAIHRNLMISIDKILN"
     gene            complement(1259..2308)
                     /locus_tag="PLS16_10095"
     CDS             complement(1259..2308)
                     /locus_tag="PLS16_10095"
                     /inference="COORDINATES: protein motif:HMM:NF014177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HNJ61475.1"
                     /translation="MFKYPKVNYIGNKEKIAKWICDQFPEDAKTLFDAFSGGCSLSYE
                     AKCRGMEVYTNDILEVNYHIANALIQNNKIVLDKNDIDIIFNGKPFEGFMFSNYSNVF
                     FFPEECKELDLYRNNIEKLDSDEKKSLAFALMRRAMIRKMPYSRFTLNWDKILQLRNE
                     EYSYQKYKRKRAYHNQSFKHHFLDNVNEYNSAIFDNQKSNIAYNDDIFNLIENVKADI
                     IYLDPPYTGTMNNYYGFYGLIDNFISSTKTTPFENNFVSKSESALLFDKLFSKLKNFK
                     YWFLSYNNSSYPRKEELLYLLNKYSDKIQVVERKHNYQITGKDKKESNKEFLFIVKNE
                     LYQENTKEIYVEAEL"
     gene            complement(2301..3287)
                     /gene="dcm"
                     /locus_tag="PLS16_10100"
     CDS             complement(2301..3287)
                     /gene="dcm"
                     /locus_tag="PLS16_10100"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HNJ61476.1"
                     /translation="MTYIDLFSGSGGFSLGFDNVGFKNIFSIDIEPSFCETYKYNFPK
                     HKLIEKDICQLSNEEIKYLKEVEDVDVVIGGPPCQGFSIAGNIGRKFIDDPRNKLFKE
                     FVRVVKVVKPKFFVMENVARLYNHNKGETRKEIINDFESLGYKVECKTLNSADYGVPQ
                     IRKRVIFIGTRFNQKIEFPKRTVDKYLTVKEALHNYPMLSAGEESIIPNHVAMTHSYQ
                     MLEKMSYISDGGSREEIPVSIRPKSGDVRKYIRYKSDEPSVCVTGDMRKIFHYEQNRA
                     LTVRELAKLQSYPDDFIFKGTKISQQQQVGNSVPPRMAEAIASVIIKMNQNV"
     gene            complement(3303..3629)
                     /locus_tag="PLS16_10105"
     CDS             complement(3303..3629)
                     /locus_tag="PLS16_10105"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNJ61477.1"
                     /translation="MNASFGEYIRELRTNKGLTLTQLASQLDLDSANLSKIENGKREF
                     DEKRLIKLATVFDLDIEKLKVEYFGDQFAKKMYAFNCSSETLIVAEKKVNYLKSVNVK
                     QTEIDF"
     gene            complement(3850..4635)
                     /locus_tag="PLS16_10110"
     CDS             complement(3850..4635)
                     /locus_tag="PLS16_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNJ61478.1"
                     /translation="MLKEITSLHNPLIKKVLLLQEKPKYRREENVFIIDGLKEIKMAI
                     DNNFDLTHIFFLSAYKNEVQALTTKAELIQLDTAVFEKIAYRGNTSKAIAIAQQKHQT
                     LSEIKCDKQAFFVVLDGVEKPGNIGAMLRIADAANVTAVLISDAKTDFYNPNVVRSSV
                     GCIFSKQIVVDTKENLLAYLKNNGVSIFTTSLKSSKDYLKVDYNTPCAIVVGTEADGV
                     NAFWEENSTQNIIIPMRGQNDSLNVSNSLAIVIFEALRQRALN"
     gene            complement(4648..5784)
                     /locus_tag="PLS16_10115"
     CDS             complement(4648..5784)
                     /locus_tag="PLS16_10115"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNJ61479.1"
                     /translation="MKKNSYLLLVLMMLFFASCSNSKKIKIEGTISNSENVLVVLEDI
                     NAQEITMIDTNRIHDKAFKIENYIKEDGLYRLSLTEDKIIYLYLKKGDDIKLDIDANN
                     FMNYKVSGNEGSQSIANLMKYVSDFSTEAESLVSAYEAAQGSAKDSIETILNNNDTKY
                     IQHLKDFIAKEENPTVATFAVNFFGPSIQEEMPYIIETVDKLASKDSKSVYIKQFQAA
                     LQSYKDALVQEEEGGLGIGSKAPNIALPNSNGDTLSLSQLQGKYVLLDFWASWCRPCR
                     DENPNVVKIYHQYKDKGFDIFSVSLDNNEKPWIAAINTDKLVWKNHVSDLKGWNSSAA
                     RLYNVESIPTTYLIDPSGKIIGKNLRGKALENKLAEVFATTAQK"
     gene            complement(5858..>6180)
                     /locus_tag="PLS16_10120"
     CDS             complement(5858..>6180)
                     /locus_tag="PLS16_10120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNJ61480.1"
                     /translation="QLKRTASAITVGISDGFQRRNRQEKARLYIEAQNFLNEIYYLLI
                     LSKDLGYISSDELLENTNEIQRMLSGLIRSVLGINKNNPPRNRDNDTDGLIAGADIDD
                     VDML"
BASE COUNT         1975 a         1018 c          816 g         2371 t
ORIGIN      
        1 cagcttggcc aagtaatgtt ccaaaagaag gcgaaggctg gaaatattaa ttctggtttg
       61 attgaacgag taataaaaat aacgaatttg gtaggagatt taagataata gagttttaaa
      121 ttaaaaatat atcacaaaag ttaatacgag atggaatatt taattaacta acgagttcta
      181 accagtatgg tgttattgct ctttctaatt caatatttta tctattgata tcattagatt
      241 tctatggata gctaaataac tttttcgttt ctcgttcaag atggatggtt caagagtata
      301 gtttttccaa aactctttta catcttcaga aattcttttt gagaacaaca ttttcccctc
      361 gtctgtaaat gaaatgtatt ttaaatcaaa taatgaatct accgttctac tcaataatag
      421 tccgttattt ggatcgtatg cttcggtttc gtccgattgt acaaatggtt taatatgact
      481 tgctataaga actggatatg ctaatttttc caatacacac aaaggcttac catagatttc
      541 ttcgcattct tcttgtaatt gatttttgta aagtcgatgt aaatatgggt ctcgtttttt
      601 agatattgca ctaaggtctt cgccaaaaat ttgttgagca tcttctttga aatacaaatc
      661 atctttaaca aaaactaaat catctaattt tccaagaatg ttatataaat atccgacttg
      721 attatatttt ctttttataa agccaatttt agttgcttct tctacatatt tttgaagttc
      781 tgattcagaa ataaatgttc tcttatgtgc ttcaatatca accaacatca atgcaataat
      841 ttcttctttt gagagttttc ctttttcaat caaagtttga attaaaaagt tgatttgctt
      901 tataggagaa tcaatattta cagctctgtt aaaacttgaa tttgagtaaa taatttttga
      961 aagtaatgtt tctcttttct tatttgttct tgcttcaata tattctttag cttgtggatg
     1021 atacgacaac aaaaaagaat tgataaatcc catttttacc aattggttta ttgatttacg
     1081 aattgaaatc agattgatag ggtttacctt ttctacctct aattgtaaac gtaaatactt
     1141 ttcatccgaa taagattctt ctctatattc atcaacgaat ttaatacaca cagcaagagt
     1201 gtccaaaaat tttttaccgt tgtaatcggt aaaagcattg gttaatttcc aatattcttc
     1261 ataattctgc ttcgacataa atttccttag tattttcttg ataaagttca ttttttacaa
     1321 taaacaaaaa ctctttatta ctttcctttt tgtcctttcc tgtaatttga taattatgtt
     1381 ttctttcaac tacttgaatc ttatcagagt atttgttcag caaataaagt aattcttctt
     1441 tacgtggata tgatgaatta ttgtaactca aaaaccaata tttgaaattt tttaattttg
     1501 aaaataattt atcaaaaagt aaagcggatt cgcttttact cacaaaatta ttttcaaatg
     1561 gtgttgtttt agttgatgaa ataaaattgt ctattaagcc ataaaatcca taataattat
     1621 tcattgttcc tgtatatggt gggtcaagat aaataatgtc ggcttttaca ttttcaatca
     1681 aattaaaaat atcatcgtta tatgcaatat tgcttttttg gttgtcaaaa atagcactat
     1741 tgtattcatt tacattatcc aaaaaatgat gtttgaatga ttgattgtga tatgctcttt
     1801 tacgtttgta tttttgataa ctatattcct catttcttaa ttgcaaaatt ttatcccaat
     1861 tcaaggtaaa acgtgaataa ggcattttgc gaatcattgc tctacgcatc aatgcgaaag
     1921 ccagagattt tttttcatcc gaatctaatt tctcaatatt attcctatat aaatctaatt
     1981 ctttacattc ttctggaaag aaaaaaacgt ttgaataatt actaaacata aagccttcaa
     2041 aaggcttacc attgaaaata atatcaatgt cattcttgtc taatactatt ttattgtttt
     2101 gtattaacgc atttgctatg tgataattta cttctaaaat gtcgtttgtg taaacttcca
     2161 ttcctctaca ttttgcttca taacttaacg aacatccacc tgaaaatgca tcaaaaagtg
     2221 tttttgcatc ttctggaaat tggtcgcata tccatttggc aattttttcc ttgttgccta
     2281 tatagtttac tttcggatat ttaaacattc tgattcattt taataattac acttgctatt
     2341 gcttctgcca ttcttggtgg aacggaattt ccaacttgtt gttgttgcga aatttttgta
     2401 cccttgaaaa taaaatcatc ggggtatgat tgtaattttg ctaattcacg aacagtcaat
     2461 gctctatttt gttcgtaatg aaaaatttta cgcatatcac ctgttacgca aacggatggt
     2521 tcatcgcttt tgtagcggat atatttccgt acatcgcccg attttggtct tatacttaca
     2581 ggaatttctt ctctactacc accgtcagaa atgtagctca ttttttcaag catttgataa
     2641 gaatgtgtca ttgcaacgtg atttggaatg attgattctt cgcctgctga aagcattgga
     2701 taattatgca acgcttcttt tacagttaaa tatttgtcaa cagttctttt aggaaactca
     2761 attttttgat taaaacgagt tcctataaaa attactcttt ttctgatttg aggaactcca
     2821 taatcagccg aattcaaggt tttacattct actttgtaac ctaaactttc aaaatcattt
     2881 ataatttctt ttctagtttc acctttgttg tgattgtaca aacgtgcaac attctccatt
     2941 acaaaaaact ttggctttac aactttcaca acacgaacaa attctttgaa tagtttgttt
     3001 cttgggtcat caataaattt tcttccaata tttcctgcaa tgctaaaacc ttgacaaggt
     3061 ggtccaccaa ttactacatc aacatcttct acttctttca aatatttaat ttcttcatta
     3121 gaaagttggc aaatatcttt ttcaattagc ttatgctttg gaaaattata tttgtaagtt
     3181 tcgcagaaac ttggctctat atcaatagaa aagatatttt taaatcctac gttatcaaaa
     3241 cccaatgaaa aaccgcccga acctgaaaat aagtctatgt atgtcattgc ttttttcata
     3301 gtttagaaat ctatttcggt ttgttttaca tttacacttt ttagatagtt cacttttttt
     3361 tcggcaacaa tcaatgtttc tgatgagcaa ttaaatgcat acattttttt tgcgaattgg
     3421 tctccaaaat attctacttt cagtttttca atatcaaggt caaatactgt agctaatttt
     3481 atcaaacgtt tttcatcaaa ttcacgtttc ccattttcta ttttgctcaa attagcagaa
     3541 tcaaggtcaa gttgagatgc aagttgtgta agggtcaaac ctttgtttgt ccgtaattct
     3601 cttatatatt cgccaaacga tgcattcatt tgcttgtaaa tttaaacttg tcaattaatg
     3661 acaaagttaa gtataatttt acaatcatac aagtttatat tggatttatg cgaacgataa
     3721 taaagtataa tggtttaggg ttaggctcag tattgtttga gtaatgacga agtgcttagt
     3781 aacagtattt tttcgacaaa tccgtagttg gacaaatctc aaaaaatata atattccact
     3841 acataattat taatttaaag cacgttgcct caaggcttcg aaaattacaa ttgctaagga
     3901 gttagaaaca ttcaatgaat cattttgtcc tcgcatgggt atgatgatat tttgagttga
     3961 attttcttcc caaaaagcat ttacaccatc agcttctgtg ccgacaacaa tagcacaagg
     4021 cgtattataa tctactttta gataatcttt agaagatttt aatgaagtcg taaaaataga
     4081 aaccccatta ttttttaaat aagctaataa attttctttt gtatcaacga caatttgctt
     4141 agagaaaata cagcctactg aagaacgcac aacattcgga ttataaaaat ctgttttggc
     4201 atctgaaatc aaaactgctg taacatttgc agcatcagca atacgcagca ttgcaccgat
     4261 atttcctggt ttttctacac catctaaaac aacaaaaaat gcttgtttgt cacattttat
     4321 ttctgaaaga gtctgatgtt tttgttgtgc aattgcaatg gctttacttg tatttccacg
     4381 atatgcaatt ttttcaaaaa ctgctgtatc taattgtatc aattctgctt tagttgttaa
     4441 tgcttggact tcatttttat atgctgaaag aaagaatata tgtgtcaaat caaaattgtt
     4501 atcaatcgcc attttgattt cctttaatcc gtcgataata aaaacatttt cttctcgtct
     4561 atacttaggc ttctcttgta ataaaagaac cttttttatc agcggattat gtaaactagt
     4621 aatttctttt agcattaaaa gagaaattta cttttgagct gttgtcgcaa atacttcggc
     4681 taatttattt tccaaagcct tgcctctcaa atttttgcca ataatttttc cagatgggtc
     4741 tatcaaataa gttgtcggaa tagattctac attatacaat cttgctgcag atgagttcca
     4801 accttttaaa tctgaaacat gatttttcca aaccaattta tctgtattga tagcagcaat
     4861 ccaaggcttt tcattattat ccaatgacac actaaaaata tcaaagcctt tatctttata
     4921 ctgatgataa atttttacaa catttgggtt ttcatctcga caaggacggc accaacttgc
     4981 ccaaaaatct aacaatacat attttccttg caattgactt aacgataaag tatctccatt
     5041 actattgggc aaagcaatat taggtgcttt tgagccaatt cctaaaccac cttcttcttc
     5101 ctgaaccaac gcatctttat aggattgtag tgcggcttga aattgtttga tataaactga
     5161 ttttgaatct tttgatgcta atttatctac agtttcaata atataaggca tttcttcttg
     5221 aatactaggt ccaaaaaaat taacagcaaa agtggctact gttgggtttt cttctttagc
     5281 aataaaatct ttcaaatgtt ggatatactt ggtatcatta ttatttaaaa tagtttcaat
     5341 agaatctttt gcactgcctt gtgctgcttc ataagcactc actaaacttt cagcctcagt
     5401 agaaaaatca gatacatatt tcatcaaatt tgcaatagat tgactgcctt catttcctga
     5461 aactttataa ttcataaagt tattagcatc aatatctaat tttatatcat cgcctttctt
     5521 taaatataaa tagatgattt tatcttcggt caaactcaat cgatacaagc catcttcttt
     5581 aatataattc tcaattttaa aagctttatc atgaattcta ttggtgtcta tcattgtaat
     5641 ttcttgtgca ttgatatctt ctaagacaac caaaacattt tctgaattgg agattgtgcc
     5701 ttctatttta atcttcttag aatttgaaca agaagcaaaa aacaacatca tcaaaaccaa
     5761 taataaataa ctattttttt tcatttgtaa ttgtatattt aatttttata agctgccaaa
     5821 atttagcagt atttgaacaa tctaaagtat aatattttta aagcatatcg acatcatcaa
     5881 tatcagcacc agcaattagt ccgtctgtat cattatctct atttcttggc ggattatttt
     5941 tattgatacc taatactgaa cgaatcaaac cactcaacat tctctgaatt tcatttgtat
     6001 tttctaataa ttcatcacta cttatatagc ctaaatcctt acttaaaatc aataaataat
     6061 agatttcatt tagaaaattt tgagcttcaa tatataatct tgctttctct tgtcggtttc
     6121 ttctttgaaa accatcagaa atcccaacag taattgcaga agcagttctt ttcaattggt
//
LOCUS       DAOQCW010000131         6178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_5262_length_6178_cov_5.876041, whole genome shotgun sequence.
ACCESSION   DAOQCW010000131 DAOQCW010000000
VERSION     DAOQCW010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6178
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5262_length_6178_cov_5.876041"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="PLS16_10125"
     CDS             <1..854
                     /locus_tag="PLS16_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61481.1"
                     /translation="GCKKQSSEETFTDLPNSGTLSFLTYNVAGLPEGINSDQFPPRHI
                     PLISPLLNNFDVVNVQEDFAYHDKLYKDITLKYKTKYVADIVFGDGLNTVSQLPILDY
                     KRMRWDSCNGTDCLTPKGFTYSRLRFNETSFIDLYNVHANAGDSEKDWAARRNNILQL
                     LTYIETHSAGKTVVLMGDFNCQYTRMGDNIKLLCDSLGFKDVWIELIRGGVYPEKGDE
                     ALKDCVVKYSSVNCEVVDKVFYRNTGNIELKPIFFDQDNPLFFDDSGMQLSDHRPMNV
                     KFEYEIK"
     gene            complement(858..5615)
                     /locus_tag="PLS16_10130"
     CDS             complement(858..5615)
                     /locus_tag="PLS16_10130"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNJ61482.1"
                     /translation="MKKVVKYIAVLISTIIFLLLFSFLTLTVLIQIPRFQTWAVQKAS
                     ILLSDKLDSKVSVGSVDIRFIKTIELNQIYLEDKHQDTLLFANKISADLLLGKSLFSQ
                     INTLKGNKIYVGDIVLDGIKFNGYRTENDSLYNFSNIIDAFKSDKKKEKKKKSNTSLD
                     LQVRNLLLSNIDLKYDDQYGGTGLKIKAKKLNVHINKLDLSKLLVDVDNIEIDNPIVA
                     LTLYNRPKKENKKPSKPFAPQNLGLLLKVGKAEITNGQYLMDMLNRKPIKVGDFKISN
                     MNIAQINTKIKDFGWDTTGLYANIKYLKTVLDNNIRIKNVSGDVLLDNKSIQLSDANI
                     QMNQSEINGNYALKFMNDWKDFSHFAQDVILSANIKESKVYRNDVIGFVPSINKYLPE
                     KAIVIANVRGPLDNLKINTINLKAGTNTKIDITGNIKGLPKIKQTLFDLYVNNIQTNP
                     NDLGSMLPFLKMPKQVTNAGNINFKGSFKGYIDDFYTKGNLITDNLGSIGADIHLQFP
                     KGNAPKYQGQIIAQKINLAELTGNQKLLGTIDLDMHALGNGFSLKNINTKLVGKVKNF
                     YLNGFVFKEIDINGILDKKKFNGNAFYDDGCFLFDFNGLADFNDKIPIYNFNIQLKNA
                     NLQDFKLAKDSVIISMNGAVKASGNNINNINGIASFDNIIIQNEKNILTLSDLSADLQ
                     SLENFKHYLIKSNEIDIDLKGNFNPLTLVPSAKVYLSKYSTLIKPSAKDFKNNALQDL
                     KAKIFMHSDFGGLFKVFLPKLNYTSELNINGGFNNSVDYFDVSVRADSINYDKIALAK
                     INLATYNQGKELLSEFSVKNIKSGKVSFDDINLDLNSSLEQLLTSISIEPNTSKNGFQ
                     MVSTLNFTNDTIIAKILDSKIKLNDKYWIIEKGNSVMLYDSIFETQGFKIVQEQQAIF
                     LQNGKNTLSDLKLTLNNINLADIAQIIDTTGSIKRGDLTGTINLKNVLKNPEIHTDIV
                     INDFKIIDYEAKYIGLDAVYGRGGKKIAEFGGVLDDPNYQLAFNGSYDMQVKGKEKLD
                     VEALIDKLDLSFLQTLLKNEISVKNTFVKGDVKVSGSLKNIVLDGKATLLDTATIKMR
                     YLGTTFRIPKDEVITLNKSGFDFGTLTVFDEIGNNASVSGKLLHNSFKNFTVDKVIFN
                     TPLGYHFMHTQFADNQDFYGDVFASGNANINGPFNDLKIEVNASTLNKTVFNLPVSGK
                     NTKANYSYITFFNPNDTTKVIQDKIKLNGISILMNIIATPDAEANIILDQSTGDKISA
                     RGSGNLTMNLDKNGKLDLNGTYTITNGNYNFKFQNIISKIFKIKNGSTITFAGDPMNA
                     MLDINAVYNVNASIRNLLNTSDSLNKNILNRSIPIDLNLLITNTLSKPDINFLVSPSN
                     NSLDAQTQDIIQMLERINNNKTEVYNQAFGLLLFNGFIPFVTGGLGEQQFSGASNSFT
                     QFFTQQISNLFTKGLEQLGLKGASLDVLLKDIESKESRQFGFSYKQELFNSRLIFTVG
                     GNVNFGTTTTDINGISTNSNNSTFTSDFVLEYLITADGRIRLKTFAKTGDFDIINQDR
                     LRTGGAISFQKDFDSLKELFTPNKKSKTSVIKK"
     gene            5666..>6178
                     /gene="tsaD"
                     /locus_tag="PLS16_10135"
     CDS             5666..>6178
                     /gene="tsaD"
                     /locus_tag="PLS16_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNJ61483.1"
                     /translation="MPTCILAIESSCDDTSCAVCVDGKILSNEVATQHIHEQYGGVVP
                     ELASRSHLKNIVPVVDIALKKAQVELKDISAVAFTRGPGLMGSLLVGGMFAKSLAQSL
                     DVPLIDVHHIQAHILAHFIDEPKPQFPFLCLTVSGGHTQLVVVKDYLEMEIIGQTIDD
                     AVGEAFDKTAK"
BASE COUNT         2005 a          908 c          929 g         2336 t
ORIGIN      
        1 taggttgtaa aaaacaaagc tcagaggaaa cttttacaga cttacctaat agtgggactt
       61 taagtttttt gacctataat gttgcaggat taccagaagg tatcaattca gaccaattcc
      121 cgccaagaca tataccatta attagtccac ttttgaataa ttttgatgtg gttaatgtac
      181 aagaagattt tgcataccat gataagctat acaaggatat taccctaaaa tataaaacaa
      241 aatatgttgc agatatagtt tttggtgatg gtttaaatac tgtttcccaa ttaccaatat
      301 tagattataa aagaatgcgt tgggatagtt gtaatggaac tgattgttta acaccaaaag
      361 gttttacata tagtagatta cgttttaatg aaacctcatt tattgatttg tataatgtac
      421 atgccaatgc gggtgattct gaaaaagatt gggcggcacg aagaaataat atccttcaat
      481 tattaactta tattgaaacg cattctgctg gaaaaacggt tgttttgatg ggtgatttta
      541 attgtcaata tacaagaatg ggagataata taaaattatt gtgcgatagt ttaggcttta
      601 aagatgtttg gatagaatta attagaggcg gtgtttatcc cgaaaaaggt gatgaagctt
      661 taaaggattg tgttgtgaaa tattctagtg ttaattgtga ggtcgttgat aaagttttct
      721 atagaaacac tggaaatatt gagcttaaac ctatattttt tgaccaagat aatcctttgt
      781 tttttgatga ttctggtatg caattatcgg atcatagacc tatgaatgtt aagtttgaat
      841 acgaaataaa ataacattta tttttttatt accgacgttt tacttttctt atttggagta
      901 aataattcct ttaaactatc aaaatctttt tggaaactga tagctccacc agttctcaat
      961 ctatcttgat tgataatatc aaaatcgcca gttttagcga atgttttcag acggattctt
     1021 ccatctgcag taattaggta ttccaacacg aaatcactcg taaatgttga gttattacta
     1081 ttggtactga taccattgat atcagtagta gttgttccaa aattgacatt tccacccaca
     1141 gtaaatatca atctgctatt gaataattct tgcttgtaac taaacccaaa ttgcctggat
     1201 tctttggatt ctatatcttt taaaagtaca tctaaacttg caccttttaa gcctaattgt
     1261 tccaatcctt tggtaaataa atttgaaatt tgttgagtga agaattgtgt aaatgaatta
     1321 cttgcaccac taaattgttg ttcgcctaaa ccacctgtta caaaaggaat aaagccattg
     1381 aacaataaca atccaaatgc ttgattgtaa acttcagttt tgttgttatt aattctttcg
     1441 agcatttgta taatatcttg cgtttgtgca tctaaagaat tattacttgg agaaactaag
     1501 aagttgatat ctggtttgga gagtgtattt gtaattaata agtttaagtc gataggaatg
     1561 gagcgattta ggatattttt atttaaggaa tctgaagtgt ttaataaatt tctgattgat
     1621 gcattgacat tataaacggc attgatatcc aacattgcat tcatagggtc gccagcaaat
     1681 gttatagtac ttccattctt tattttgaaa attttggaga taatattttg gaatttaaaa
     1741 ttgtaatttc cattcgtaat tgtgtaagtt ccattcaaat ctagtttgcc atttttatct
     1801 aaattcatag tgagatttcc acttcctctt gcagatattt tatcgccagt actttggtct
     1861 aaaataatat ttgcttcagc atctggggtg gctatgatat tcattaaaat actaatacca
     1921 ttgagtttta ttttgtcttg aataactttt gtggtgtcat tagggttaaa gaatgtgata
     1981 tagctataat ttgcttttgt atttttacca cttacaggca aattaaaaac agttttgttg
     2041 agcgtacttg cattaacttc aatcttcaaa tcattaaatg gaccatttat attagcattt
     2101 ccacttgcaa aaacatcgcc ataaaaatct tggttgtcag caaattgagt atgcataaaa
     2161 tgatagccta atggtgtatt gaaaattact ttatccactg tgaaattctt aaagctattg
     2221 tgtaataatt ttccacttac agaagcatta ttgcctattt catcaaatac agttaaagtt
     2281 ccaaaatcaa aaccactttt gttgagtgta attacttcat cttttggaat tctaaaagta
     2341 gtacctaaat aacgcatttt aattgtagct gtatctaata aggttgcttt tccatctaat
     2401 acgatatttt ttagtgaacc agaaactttt acatcgccct ttacaaaagt attttttaca
     2461 ctgatttcat ttttcaaaag tgtttgtaaa aagctcaagt ccaatttgtc aattagtgct
     2521 tcgacatcta atttttcttt tccttttact tgcatatcgt agcttccatt gaatgcaagt
     2581 tgataattag ggtcgtctaa aacaccgcca aattctgcta tttttttgcc acctcgacca
     2641 taaacggcat ctaaaccaat atattttgct tcataatcaa taattttaaa atcattgatg
     2701 actatatcag tatgaatttc tgggtttttt aagacatttt tcaaattgat agttcctgtc
     2761 aaatcgcctc tttttatcga gcctgttgtg tcaataattt gtgcaatatc cgctaagttg
     2821 atgttgttta aagtgagttt caaatcagat aatgtgtttt taccattttg taaaaatata
     2881 gcttgttgct cttgaactat tttaaaacct tgtgtttcaa atatgctatc gtaaagcatt
     2941 acactattcc ctttttcaat aatccaatat ttatcgttca attttatttt agaatctaaa
     3001 attttagcaa taattgtatc attggtaaag tttaaagttg aaaccatttg gaacccattt
     3061 tttgatgtat ttggttcaat tgaaatgctt gtcaacaatt gttctaaaga gctatttaag
     3121 tctagattga tatcatcaaa agatacctta ccagatttta tatttttaac tgaaaactct
     3181 gataataatt ctttcccttg attataagtt gctaaattaa ttttagctaa tgctatttta
     3241 tcataattga tagaatccgc tcgaacacta acatcaaaat aatcaacaga attattaaat
     3301 ccaccattaa tatttaattc gctagtataa tttaattttg gcaaaaatac tttgaataat
     3361 ccgccaaaat ctgaatgcat aaatatttta gcttttaaat cttgaagagc attgttttta
     3421 aaatctttag cacttggttt aattaatgtt gaatatttac tcaaataaac ttttgctgaa
     3481 ggcaccaacg ttaatggatt aaaattaccc ttcaaatcaa tatctatttc atttgattta
     3541 ataagataat gtttgaaatt ttctaagctt tgcaagtcag cagataaatc agataaggtt
     3601 agtatatttt tttcattttg aataataatg ttgtcaaagc ttgctatgcc attaatatta
     3661 ttaatgttat ttccgcttgc ttttactgca ccattcatgg atataataac tgaatctttc
     3721 gctaatttga aatcttgaag attggcattt ttaagctgaa tattaaagtt ataaattgga
     3781 attttatcgt taaaatcagc taatccgtta aagtcaaaca agaaacatcc atcatcataa
     3841 aatgcatttc cattaaattt ctttttgtcc aatatcccat taatgtcaat ttctttaaag
     3901 acaaaaccat tcagataaaa attctttact ttacctacca attttgtgtt gatattttta
     3961 agactgaacc cattgccaag tgcatgcata tctaaatcta tcgtacctaa taatttttga
     4021 ttgccagtaa gttctgccaa atttattttt tgagctataa tttgaccttg atattttggg
     4081 gcattacctt taggaaattg taagtgaata tcagctccaa tcgaacctaa attgtctgtt
     4141 attaaattgc ctttagtata aaaatcatca atatatcctt tgaaactacc tttgaaatta
     4201 atatttccgg cattggttac ttgcttaggc atttttaaaa atggcaacat agagcctaaa
     4261 tcgtttggat tggtttgtat attattgaca tacaaatcaa ataaagtttg tttgattttt
     4321 ggcaaacctt tgatatttcc agtaatatca atttttgtat tagttcctgc ttttaagttt
     4381 attgtattta tttttaaatt gtctaatgga ccacgaacat tagcgattac aatagctttt
     4441 tcaggtagat atttatttat agaaggaaca aacccaatga catcatttct ataaacttta
     4501 ctttctttga tattggcgga taaaataaca tcttgagcaa aatgagaaaa atctttccaa
     4561 tcattcataa atttaagtgc ataatttcca ttgatttcac tctgattcat ttgaatattt
     4621 gcatcactta attggataga tttattgtct aaaagcacat cgccagatac attttttatc
     4681 ctgatattgt tatctaaaac agtttttaga tatttgatat tggcatataa accagtagta
     4741 tcccaaccaa aatcctttat ttttgtattg atttgggcaa tattcatatt gctgatttta
     4801 aaatcgccaa ctttaatagg tttcctattc aacatatcca ttaaatattg cccatttgta
     4861 atttcagcct ttccaacttt taaaagcaaa cctaaatttt gaggtgcaaa tggtttgctt
     4921 ggttttttat tttctttttt tggtctattg taaagtgtta aagcaacaat agggttatca
     4981 atttcaatat tatctacatc aactaataat ttgctaaggt ctaatttatt aatatgtacg
     5041 ttaagctttt tggcttttat tttcaagcca gtaccaccat attggtcgtc atattttaaa
     5101 tcgatattgg ataaaagtaa attgcgaact tgtaaatcta aactagtatt ggattttttc
     5161 tttttttctt ttttcttatc agacttaaaa gcatcaataa tgttggagaa attatataaa
     5221 ctatcatttt cggttctata accattgaat tttataccat ctaaaacgat atcgcccaca
     5281 tagattttat ttccttttaa agtattgatt tgtgagaaaa gcgatttgcc aagtaaaagg
     5341 tctgcactaa ttttattggc aaataataaa gtatcttggt gtttatcttc taaatagatt
     5401 tggttgagtt ctatagtttt tataaatcga atatctaccg atccgacact aactttactg
     5461 tctagtttat cagaaagtaa aatgcttgct ttttgtactg cccaagtttg aaatctaggg
     5521 atttgtataa gcacagttaa tgtaagaaag ctgaaaagca acaaaaatat aatggtacta
     5581 attaatactg cgatatattt tacaactttt ttcatagtac ctttgtagga atagatacaa
     5641 aaataagtta tttattacaa aaacaatgcc tacatgtata ttagcaatag aatcttcatg
     5701 tgacgacact tcgtgtgcgg tttgtgttga tggcaaaata ttatcaaatg aagtagcaac
     5761 tcagcatatt cacgaacaat atggtggtgt agtgccagag ttggcaagtc gttctcatct
     5821 taaaaatatt gtaccagtag tcgatattgc attgaaaaaa gcacaagttg aactgaaaga
     5881 tattagtgca gttgctttta cacgagggcc aggattgatg ggttctttgt tggtcggcgg
     5941 aatgtttgca aagtctttgg ctcaaagttt ggacgtacct cttattgatg tgcatcatat
     6001 tcaagcacat attttagctc attttattga tgaacctaag cctcaatttc cttttttatg
     6061 tttaacggtt tccggaggac atacacaatt ggtagtcgtt aaagattatt tagaaatgga
     6121 aatcattgga cagactatcg atgatgctgt gggagaggcg tttgataaga ccgcaaaa
//
LOCUS       DAOQCW010000132         6008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_5507_length_6008_cov_5.309592, whole genome shotgun sequence.
ACCESSION   DAOQCW010000132 DAOQCW010000000
VERSION     DAOQCW010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5507_length_6008_cov_5.309592"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..599)
                     /locus_tag="PLS16_10140"
     CDS             complement(111..599)
                     /locus_tag="PLS16_10140"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNJ61484.1"
                     /translation="MKRLNLKNIILVLVGCLWLNFLQAQTSINTSGGNAIGTGGAVAS
                     SIGQLFYTSDKSSSALIEAGVQHAYEIYLLKVKGNVLTVSLSVYPNPTTNQLALKVEG
                     LKNEQLSFQLLDLQGRLLSADKVVNAETVIQMSHLASAAYLLNVVDEKTGGMKSFKII
                     KN"
     gene            complement(604..903)
                     /locus_tag="PLS16_10145"
     CDS             complement(604..903)
                     /locus_tag="PLS16_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61485.1"
                     /translation="MKTQIQYLYIFFPVISITEEVANNKNPHPLKEDIIDIESKNIYA
                     FSGSIPFSKKTYYYIVQNKIQKEDNLKALDKLNYSCNEEGLVLNIQFLQFLILNK"
     gene            1100..3094
                     /locus_tag="PLS16_10150"
     CDS             1100..3094
                     /locus_tag="PLS16_10150"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNJ61486.1"
                     /translation="MYNKLLVLLFVLFISTPLLAQINHIKKALQDPKLSAKKRIDYFI
                     KLSKVYEDIQQDSAIVYANLAYALAENNKNQSSLAYAIIQKSNILINLSKEDEAISIL
                     EKAQVINLQEKNKNLEATIYNALGKAFNKKPDSAILFYNKSIELAKEIKDNDLVIKNK
                     IRLALILLHQKRHQELAKLFDKILPELQKKNDINSLIEVYTYMAFAFRDLGEQDKALY
                     YAKKSLEYTETSENKRLKAFVLSSIGSGIISYFETFEKAIPLVRKGLALATEINDKIL
                     IQDCQKRLAILYYNNDEYGKAASIVDNLLPTTKDPDIIELKASILFENGNFAEADTYF
                     AKAYKLYEKDKAYIQQKLLLQNKIDNKLASIGDESLTRDFTLLDSLSTLIYDTESTEQ
                     FFDLETKYRTLEKEAEINKKELALAQSKLKIWYISGLALLLLIVWFFSILFLRNKHKR
                     KDLEDANLILELKHNLNSSELSNINNQLNPHEIKNLITSFAPDLITKAPEAYKKMIKL
                     FNVTRASLSDKITEPLDIQLQQAKDYLELQQSISPYLWNFNFDIEIQNLNFELPRLLL
                     KNMIENAVKYGMKSIKENGQINLIVSIEQQLIKIRIKDNGIGIDNNRSEGSTGIGLSA
                     YKRFFDLINAQNKRKASLELRRENSWTIVEISIPLEYQYI"
     gene            3098..3874
                     /locus_tag="PLS16_10155"
     CDS             3098..3874
                     /locus_tag="PLS16_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNJ61487.1"
                     /translation="MVYKYLVIEDNPREIELLDILMQEYPEFQKTIVTCDFKEGIKEI
                     LKQKPDLVFLDVELPDFTGFELLKELQNHLQELPEIIMATAHEKYAIDAVNKDILYYL
                     LKPIDPDELIIAINRFYIKKAKTNKSITIKTNKGFSFLNFDDIFLIKSASNYTCFYTR
                     TLQQIVVSKTMKEYELFLNEDFLRIHKSYTINTKYIRFLNVGKKRVQLSIPELKNLDA
                     KDNFFDHFLDSLCEENNIELPIGDVYMEKVKNSVLYSKIN"
     gene            complement(3886..5175)
                     /locus_tag="PLS16_10160"
     CDS             complement(3886..5175)
                     /locus_tag="PLS16_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HNJ61488.1"
                     /translation="MKYDTINQELFVANRKKFAAKLAPNSMAILFANDEMPRSADQSF
                     PFRQNPDLFWLSGIDQEQTILIIYPDCPNPIYREALFIRKTNAHIAVWEGHKYTQAEA
                     QQSSGIDTISWNDTFDAVIGTLMSYCENVYININENDRASNVVPYKDIRFANELRNNY
                     PAHQVKRLGPIMAELRAVKHEIEVEIIKKACGITRDAFIRVLKFMQTGIKEYEVEAEI
                     IHEFIRQGANGHAYSPIIASGKNACVLHYVDNNQICKDGDLVLMDFGAEYANYASDMT
                     RTIPVNGKFSPRQKEVYNATLLVMNQAKELLRPGKTLADYNAEVGLIMEEQLIKIGLL
                     NKDEVAKQDKNNPLYKKYFMHGTSHFMGIDVHDIGNRYAPMQVGNVFTCEPGIYIPEE
                     NIGIRIENDIYIGENGNIDLMADIPIEVEEIEMLMRK"
     gene            complement(5247..5894)
                     /locus_tag="PLS16_10165"
     CDS             complement(5247..5894)
                     /locus_tag="PLS16_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61489.1"
                     /translation="MRKYYVLYRFYVAFALIAVGILIHLTVHKGWQWSWIFYTLGLGT
                     ILWDIFIGPMMYLQKLVTDGDVDGAKDILNSIKFPNLLIKPVRQGYYMLKSNFDMANQ
                     NLDDAEANIRKSLANKSSLVPQQEGVSYLQLGTIAMQKGDSKEALKNLKIALQKGLPD
                     DDNKATAYLQLCSIYAQRRDVKTAKNYLRQANACKIKNDEIKKQLKEINKQLARA"
BASE COUNT         2181 a          954 c          817 g         2056 t
ORIGIN      
        1 ctcctccatc acgaatgaca gcttgataac tcatcttttg tggcgactga gcaataacta
       61 ctgaaactaa tagcattgct agaattgatg taattaactt tctcatatac ttaatttttt
      121 attattttaa atgatttcat tccacctgtc ttttcatcta caacattaag caaatatgca
      181 gcacttgcta aatgactcat ttgtatcaca gtttccgcat tgacaacttt atctgctgat
      241 aataatctgc cttgtaaatc aagcaactga aaacttaact gttcattttt taagccttct
      301 acttttaatg ccagttgatt ggtagtagga ttaggataaa cggaaagtga aaccgttaga
      361 acattccctt ttactttgag taaataaatc tcataagcat gttgtacgcc tgcctcaatt
      421 aaagcagaac tacttttgtc tgatgtgtaa aataactgac ctatacttga agctactgca
      481 cctcctgtac ctattgcatt gccccctgat gtattaattg aagtttgtgc ttgaagaaaa
      541 tttaaccata aacaacctac aagtactaga ataatatttt taagatttaa ccttttcata
      601 cgcttattta tttaatatta aaaattggag gaattggata tttaaaacca atccctcctc
      661 attgcaagag taatttagtt tatctaaagc ttttaaatta tcttcttttt gaatcttatt
      721 ttgtactata taataatatg ttttcttaga aaaaggaata cttcctgaaa aggcataaat
      781 gtttttagac tctatatcaa tgatatcttc tttgagtgga tgagggttct tgttattagc
      841 aacttcttca gttatagaaa ttactggaaa gaagatatat agatattgaa tttgtgtttt
      901 cattgactta aatcattaat taaagtgtat atagcttaaa taattctttt tagtaaaatt
      961 atatcctact atacatattt gtggatttta gctgatttat tgagtgaact acccaaaata
     1021 gcgtgtgaac acataaatat taaataaaaa aagaggttta ttttatatat ctttacctag
     1081 agtaggtaat tttagtctaa tgtataataa gttgttagtt ttattatttg tactatttat
     1141 ttcaacacct ttgttggcac aaattaatca tattaaaaaa gcactccaag acccaaagct
     1201 ttctgctaaa aaacgtatag actactttat caaactttca aaagtttatg aggatataca
     1261 acaagacagt gctattgttt atgccaactt agcctacgca ttagctgaaa acaataaaaa
     1321 tcaaagcagt ttagcttatg caattattca aaaaagtaat attctaatta acctatctaa
     1381 agaagatgag gcgataagca ttttagaaaa ggctcaggtt attaatcttc aggaaaaaaa
     1441 taaaaactta gaagctacta tttacaatgc tcttggcaaa gcctttaata agaaacctga
     1501 ctctgcaatt ttattttaca ataaaagtat tgaactagct aaagagataa aggataatga
     1561 cttagttatt aaaaacaaga taaggttagc actcatttta ttacaccaaa aaagacacca
     1621 agagttagct aaacttttcg acaaaatact tcctgaattg caaaagaaaa atgacatcaa
     1681 tagtttgatt gaagtttata cttatatggc atttgccttt agagatttgg gggagcaaga
     1741 taaagcattg tattatgcaa agaagtcatt agaatatact gaaacttctg agaataaaag
     1801 gctaaaagct tttgtattaa gtagtatagg aagtggaatt attagctatt ttgaaacatt
     1861 tgaaaaggct attcctttag tacgtaaagg tttggcttta gccactgaaa taaatgacaa
     1921 aatacttata caagattgcc aaaaaagatt agctatttta tattataata atgacgaata
     1981 tggcaaggct gcatctatcg ttgataactt actgccaact acaaaagacc ctgatattat
     2041 tgaacttaaa gcttctattt tgtttgaaaa tggaaatttt gcagaagcag atacttactt
     2101 tgcaaaagcg tacaaattat acgaaaaaga caaagcctat attcaacaga aattattact
     2161 acaaaataaa atagacaaca agcttgcatc tattggtgat gagagtttaa ccagagattt
     2221 tactctttta gattcactct caacattaat ttatgataca gaaagtactg aacaattttt
     2281 tgatttagaa actaaatata gaacgcttga gaaagaagcg gagataaata aaaaagaatt
     2341 agcattagca caatcaaaat taaaaatttg gtatatctct ggtttagcac tacttctgtt
     2401 aatcgtctgg tttttcagca tactattttt aagaaacaaa cacaaaagaa aagacttaga
     2461 agatgccaac cttatcttag aattaaaaca taatcttaat tcaagtgaat tatctaatat
     2521 caataatcaa cttaatccac atgagataaa aaacctaatt actagttttg ctcctgactt
     2581 aatcacgaaa gcacctgaag cttataaaaa gatgataaaa ctttttaatg ttacgagagc
     2641 ttcattaagc gataaaatta cagaaccctt agatattcaa ctacagcaag caaaagatta
     2701 tctcgaatta caacaaagta tcagtccata tttatggaat tttaatttcg atattgaaat
     2761 acaaaattta aactttgaac ttccaaggtt attattaaaa aacatgattg aaaatgctgt
     2821 taaatatggt atgaaatcaa taaaagaaaa tggtcagatt aacttgatag tttctataga
     2881 acaacagtta ataaagataa gaattaaaga caacggaatt ggcattgata ataatagaag
     2941 cgagggaagt actggaatcg gcttatctgc gtataaacgc ttttttgatt taataaatgc
     3001 acaaaacaaa agaaaagcca gtcttgaatt gagaagagaa aattcttgga ctatcgtaga
     3061 aatttcaata cctttagaat atcaatatat ttaagatatg gtgtataaat atttagtaat
     3121 tgaagataac ccaagagaaa ttgagctatt ggatatttta atgcaagagt atcctgaatt
     3181 tcaaaaaacc attgttacct gcgatttcaa agaagggatt aaagaaatac taaaacaaaa
     3241 acctgatttg gtatttttag atgtagaatt acctgatttt acaggatttg aattattaaa
     3301 agaacttcaa aaccatttgc aagaattacc tgaaatcatt atggctacag ctcacgaaaa
     3361 atatgccatt gatgctgtaa acaaagatat tttatactat ttattaaaac caatagaccc
     3421 agatgaatta attattgcta taaatcgatt ttatattaaa aaagctaaaa caaataaatc
     3481 tattactata aaaacaaata aaggtttttc atttttaaac ttcgatgata tatttttaat
     3541 caaatctgct tcaaactata cttgttttta taccagaaca ctccaacaaa ttgttgtgag
     3601 caaaacaatg aaagaatatg aactttttct taatgaagat tttttaagaa tacacaaaag
     3661 ctatacaatc aatacaaagt atattcgttt cttaaatgta ggcaagaaaa gagtgcaatt
     3721 atcaattcct gaattaaaaa atttagatgc taaagataat ttttttgatc actttttaga
     3781 ctcattatgc gaggaaaata atatagaatt accaatcggt gatgtctata tggagaaagt
     3841 taagaactcc gttttatata gtaaaatcaa ttagaaagat aatttttatt tcctcatcaa
     3901 catttctatt tcttctactt caataggaat atctgccatc aaatcgatat ttccattttc
     3961 acctatataa atatcatttt caattctaat tccaatattt tcttctggaa tataaatacc
     4021 tggttcgcaa gtgaacacat ttcctacttg catcggtgca tatctgttac caatatcatg
     4081 cacatcaatt cccataaaat gagaggtgcc atgcataaaa tatttcttgt acaatggatt
     4141 atttttatct tgctttgcca cctcgtcctt attgagtaag ccaatcttaa ttaattgctc
     4201 ttccattatc aaaccaactt ctgcattata atccgctaaa gttttgcctg gtcgtaataa
     4261 ttcttttgct tgattcatca ccaataaagt agcattatat acttcctttt gtcttggaga
     4321 aaatttgcca tttacaggaa ttgttctcgt catatccgat gcataattgg catattcagc
     4381 tccaaaatcc atcaaaacca aatcgccatc tttgcaaatt tgattattat ccacataatg
     4441 taaaacacaa gcattttttc ctgatgcaat aattggcgaa taagcgtgtc catttgcacc
     4501 ttggcgtata aactcatgaa taatttctgc ctccacttca tactctttaa tgccagtttg
     4561 cataaacttc agcactctaa taaatgcatc tctagtgatt ccacaagcct ttttgataat
     4621 ttctacttcg atttcatgtt ttacagcacg caattcagcc ataattggtc ccaatctttt
     4681 tacttgatgt gcaggataat tatttcttag ttcattagca aatcgaatat ctttataggg
     4741 tacaacatta ctcgctctat cattttcatt aatattgata taaacattct cacaataaga
     4801 catcaatgtt ccaataacag catcaaaagt atcattccaa gaaatagtat caattccaga
     4861 agattgttgt gcctcagctt gcgtatattt atgtccttcc caaacagcaa tatgagcatt
     4921 tgtttttcta ataaataatg cttctcgata aataggattt ggacaatcag gataaattat
     4981 taatatcgtt tgttcttggt caatgccaga aagccaaaat aaatctggat tttgtcgaaa
     5041 ggggaaactt tgatcggcac ttcttggcat ttcatcattt gcaaacaaaa ttgccattga
     5101 gtttggagct aattttgctg caaacttttt tctattagct acaaataatt cctgatttat
     5161 tgtatcgtat ttcatttgag ttggtattaa aattaaaata gtaagtcaag ttctattacg
     5221 acttatttct tactacctat tataaattaa gcccttgcca attgtttatt aatctctttc
     5281 aattgtttct taatttcatc attttttatc ttacaagcat ttgcttgacg aagataattt
     5341 ttagcggttt ttacatcacg tctttgagca taaattgaac ataattgaag ataggcagtt
     5401 gctttattat catcatctgg caagcctttt tgcaaagcaa tttttaagtt tttcaaagct
     5461 tctttactat cgcctttttg catagcaatc gtgcctaatt gcagataaga aacaccttct
     5521 tgttgcggaa ctaagctact tttattcgct aaacttttac gaatatttgc ttctgcatca
     5581 tctaaatttt ggtttgccat atcaaaatta gacttcaaca tataatatcc ttgacgaact
     5641 ggtttgatga gtaaattagg aaacttaatg ctattcaaaa tatcttttgc tccatcaaca
     5701 tcgccatcgg ttactaattt ttgtaaatac atcattggcc ctataaaaat atcccaaaga
     5761 atagtaccca aacctaatgt ataaaaaatc caagaccact gccaaccttt atgtacagtt
     5821 aagtgaatta aaatacccac agcaattaac gcaaatgcca cataaaaacg gtacaaaaca
     5881 taatattttc tcatagtgca aaaataatgc ttttattttt ataatgtact atttataaat
     5941 tggtgaatgc ttaaaataaa aactctccaa tacaaaatat tggagagcaa cacataaaaa
     6001 cccaatgt
//
LOCUS       DAOQCW010000133         5950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_5610_length_5950_cov_5.017472, whole genome shotgun sequence.
ACCESSION   DAOQCW010000133 DAOQCW010000000
VERSION     DAOQCW010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5950
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5610_length_5950_cov_5.017472"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PLS16_10170"
     CDS             <1..225
                     /locus_tag="PLS16_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61490.1"
                     /translation="ALGGMLFGTLFGVLIIPGLYYVFAMLSDGKKLIDDEEQHSLSED
                     LVTGENRDKLNKRVKKIIERILRRNNKGAE"
     gene            321..1760
                     /locus_tag="PLS16_10175"
     CDS             321..1760
                     /locus_tag="PLS16_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNJ61491.1"
                     /translation="MNNKRIKQIVLLLSALLIFNSCKIPKIVQRTENKITPNTYNASN
                     NIDTVNTADISWRTFFTDSNLVKLIDTALVNNQELMITLQEIEISRNEIRSKKGALLP
                     FVTGNLGIGGEKVGRYTSKGASDAAHDIAPEKEIPEFLPDFKLGLAMDWEIDIWKKLR
                     NAKKAAVKRYLASVEGKNFVLSNLIAEIANSYYELVALDNQLEAVRQNIQIQTKALEV
                     VKILKDAARANELGVQKFEAEVYGTQSKEYEILQEIKVTENRINRLLGRFPQPIVRNS
                     ENFTQMLPPIVHTGIPAQLLANRPDIKQAELELDASKLDVKVAKAEFYPALKITAEIG
                     IDAFRPSFWVKMPESIFSSLAGSFLAPLFNRNAIMAEYLNANAKQKQAVFNYEKCIIN
                     GYYDVSTQIYNIDNLDKNFKLKAQQINVLDSAVTTANDLLQSSRADYFEVLMTQRDVL
                     ESKLELIETKKAQLNAVIEIYRSLGGGWK"
     gene            1896..2513
                     /locus_tag="PLS16_10180"
     CDS             1896..2513
                     /locus_tag="PLS16_10180"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNJ61492.1"
                     /translation="MVSKEESILFSAERLFAEKGFDGTSTREISKAADANISLIAYYF
                     GSKEKLLESIFEYRIKESLDFALTLMLKEDLSAWQKLELLIDKFVDRVRRLKYFYLLV
                     QREKLNLSNPAIIEIIRNYHKEFLKIYQTILKDGIKQGVLKTNYSLELIHFTISGTLF
                     NSINALELKKTTIDFTDKMEQKYYSDLKKHLKILLKHLLGYEGKI"
     gene            2497..3807
                     /locus_tag="PLS16_10185"
     CDS             2497..3807
                     /locus_tag="PLS16_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNJ61493.1"
                     /translation="MKGKYKILLASILLLVGINNLFAQEVKMISLEEAIQLGVKNSKS
                     LIIDQAKIDEALANYSKAKNNKLPDLKLSGSAMALSKGKMNLEMMPNTGNSPKPNSAF
                     MGSANFSFPIYAGGRIKNGIKITEYLLEATKLSAENDKTAIAYNIAQAYNNLFKVNQI
                     INVLSENLKAAKERDKAFLNLDNNGVLARNDRLKANLQTSNIELQLLEAQNNFDIANV
                     SMNLLLGLPVETQLQVDSVYIQSTEDSISLNFYLEQAFKNRKDLQAIALQKKAAETQS
                     KAAKAHLIPTIALTAGYIAADVPQILSMYNMANIGIGVQYDLGNVWKKNTDMMNANAQ
                     IKKIEAMNGMTQDQIQLEVVRDYQAYNLASDKIKVFEKSLEQANENFRITQNKFDNGL
                     ETITNLLEADAAQITANINVLNAKADKSLLYRKLLQTTGILITE"
     gene            3818..4921
                     /locus_tag="PLS16_10190"
     CDS             3818..4921
                     /locus_tag="PLS16_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HNJ61494.1"
                     /translation="MENITPQSPQSEKKKNILFPIILAIAILAGVFFGYKEYVHGQTH
                     EKTDNAQVMSNMTPIISRVSGYINEVKVTDNQFVHKGDTLLVLDNRDQKMLLAQTEAA
                     YITAQNNISVAKLSANAASKNINTSNAAVNTADAQIDLAKVNVWKTTQDFNRYANLIK
                     DHSITQQQYEQAVAAKEAAEKQLKVLMDQKNQVKQQTEVVVSQTQTANQQISTADAML
                     KQRMVDVENAKLNLSYTVITAPEDGFVSKVPVQNGQLIQAGMQLFSLIKDNDVWIVAN
                     FKETQISKMLEGQKVEIEIDAFPKQKFEGIVSSFSPGTGSSFSVLPPDNASGNFVKVV
                     QRVPVRIDFKNLDNAIAKKMRVGLNATVEVILK"
     gene            4943..>5950
                     /locus_tag="PLS16_10195"
     CDS             4943..>5950
                     /locus_tag="PLS16_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HNJ61495.1"
                     /translation="MEQDSLVEYGFRRAIITLTTILCALLEIVDSTIVNVALNEMKGN
                     LGATLSEVGWVVTAYAIGNVIIVPMTSWLSQQFGRRNYFAASIILFTVFSFMCGNATN
                     IWELVFFRFMQGLGGGALLVTSQTIITETYPIEKRGMAMAIYGLGVIIGPTLGPPLGG
                     YIVDNYNWPYIFYINIPIGIVAAILTLRYIKSPRYAEKRAVRDIDWLGIILLAIFVGS
                     LQFVLERGHEDDWFDSTSIIIFTITATLGFITFIWRELTYKYPIVDLRVLKNVNLRIG
                     TFMSFILGFGLYGSTFIIPIYTQGILGWTALQAGALMIPSAITTAFMMPIVGKLITNG
                     VK"
BASE COUNT         2229 a          760 c         1041 g         1920 t
ORIGIN      
        1 gcattgggtg gtatgttgtt tggtactttg tttggtgttt tgattattcc aggtttatac
       61 tatgtctttg caatgctttc agatggtaaa aaattaattg atgatgaaga gcaacactca
      121 ctaagcgagg atttagttac aggcgaaaat agagacaaat taaataaacg tgttaagaaa
      181 ataattgaaa gaatattaag aagaaacaac aaaggagcgg aataatttct atatggagaa
      241 cagataaaaa aatctgttct ccaattttag ttgaaaataa attaaatatt caaaaaaaga
      301 caaataagaa tttataagtt atgaataata aacgaatcaa acaaatagtg ctattattat
      361 cagctttgct gatttttaat agttgcaaaa tacctaaaat tgtacagcgt actgaaaata
      421 aaatcacacc gaatacttac aatgcaagta ataatattga tactgtaaat actgctgata
      481 ttagttggcg aacatttttt acagattcta atttagttaa actgattgat acagctcttg
      541 taaacaacca agagttaatg attaccttgc aagaaattga gatttctaga aatgaaattc
      601 gttcaaaaaa aggagcctta ctaccatttg taactggaaa tttaggtatt ggtggagaga
      661 aagttggtcg atataccagc aaaggtgcta gcgatgcggc acatgatata gcacctgaaa
      721 aagaaatacc tgaatttttg ccagatttta aattaggttt agcaatggat tgggaaatcg
      781 acatctggaa aaaattgagg aatgcaaaaa aagctgcggt taaaagatat ttagctagtg
      841 tggaaggtaa gaattttgta ctcagtaacc tcattgctga aattgcaaat tcttactatg
      901 aattagttgc tttagataat caattagagg cagttcgtca aaatattcaa attcaaacaa
      961 aagcattgga agttgtgaaa attttgaaag atgcagcacg tgcaaatgag ttgggcgttc
     1021 agaaatttga agctgaagta tatggcactc aaagtaaaga gtatgaaatt ttgcaagaaa
     1081 ttaaagtaac agaaaataga atcaatcgtt tattaggtcg ctttccacaa ccaattgtca
     1141 gaaactcaga gaattttacg caaatgcttc ctccaattgt acatacagga attcccgctc
     1201 agttgttagc caacagacca gatattaaac aagcggaatt ggaattagat gcttctaagt
     1261 tagatgtaaa agtggctaag gctgaatttt atccagcttt aaaaataact gcagaaattg
     1321 gaatcgatgc ttttcgtcca tcattttggg ttaaaatgcc agagtcaatt tttagctcat
     1381 tggcaggtag ttttttagca cctttattca atagaaatgc tatcatggct gaatatttga
     1441 atgcgaatgc aaaacaaaaa caagcagttt ttaattatga aaaatgtatt attaatggat
     1501 attatgatgt ttctacacaa atttataaca ttgataattt ggacaaaaat tttaaattga
     1561 aagctcaaca aattaatgtt ttagattctg cggttactac tgcaaatgat ttattacaat
     1621 cttctcgtgc tgattatttt gaagtattaa tgactcaacg tgatgttttg gaatctaaat
     1681 tagaattaat agaaaccaaa aaagctcaac ttaatgcagt aatagaaatt tacagaagtt
     1741 tgggtggagg ttggaaataa tttgacaact taaaaatttt atacctaatt caaaaaagaa
     1801 ttaaaattgt ttttggatta ggttttttaa tttaagatta aatattaatc aaacgattga
     1861 ttaatttatt ttcagatatt atttttgttt tcgaaatggt ttcaaaagaa gaaagtatat
     1921 tatttagtgc cgaaagactt tttgctgaaa agggttttga tggcacatct actcgagaaa
     1981 tatctaaggc tgctgatgca aatatttctt taatagctta ttattttggt tcaaaagaaa
     2041 aactattaga aagtatattt gaatatagaa taaaagagag cttggatttt gcattaacct
     2101 tgatgctaaa agaagattta tccgcttggc aaaaattaga attattgata gataaatttg
     2161 ttgaccgtgt tagacgattg aaatattttt atttattggt acaaagagaa aagttaaacc
     2221 tttctaatcc agcaataatt gaaattatta gaaattacca taaagagttt cttaaaatct
     2281 atcagactat actaaaagac ggcattaaac aaggtgtatt aaaaactaat tattctttgg
     2341 aattaataca ttttactatt tcaggaacat tgtttaatag tattaatgca ttagaattga
     2401 aaaaaacaac tatagatttt acagacaaga tggaacagaa atattattct gatttaaaaa
     2461 aacatttaaa aatattatta aagcatttgt taggatatga agggaaaata taaaatttta
     2521 ctagcaagta tattgctttt agttggaata aataacttat ttgctcaaga agtaaagatg
     2581 atttctttag aggaagcaat acaattagga gttaaaaatt ccaaatcatt aattatagat
     2641 caagctaaaa ttgatgaagc actagcaaat tatagtaaag caaaaaacaa taaactgcct
     2701 gatttgaaac tgtcaggttc tgcaatggca ttatctaaag gaaaaatgaa tttagaaatg
     2761 atgcctaata ctggaaatag cccaaaacct aattcagcat ttatgggaag tgctaatttt
     2821 tcttttccaa tttatgcagg aggcagaatt aaaaatggta tcaaaataac tgaatattta
     2881 ttagaagcta caaagctttc tgccgaaaat gataaaacag caatagcgta taatatagct
     2941 caagcttata ataatttatt taaagtcaat caaattatta atgttttaag tgaaaattta
     3001 aaagctgcca aagagcgtga taaagctttt ttaaatttgg ataataacgg tgtattagca
     3061 agaaatgata gattgaaagc taatttacaa acttcaaata ttgaactcca attattggaa
     3121 gctcaaaata attttgatat agcaaatgta agtatgaatt tgttgttagg acttcctgta
     3181 gaaacacaac ttcaagtcga ttctgtttat attcaaagta ccgaagatag tatttcttta
     3241 aatttttatt tagaacaagc ttttaaaaat aggaaagatt tacaagcgat tgctttacaa
     3301 aaaaaggcgg cagaaacaca atcaaaggcc gcaaaagcac atttaatccc aacaattgca
     3361 ttgacagcag gttatattgc agcagatgtt cctcaaattt taagtatgta taatatggca
     3421 aatattggta tcggcgttca atatgatttg ggtaatgtat ggaaaaaaaa tacggatatg
     3481 atgaatgcta atgctcaaat taaaaagatt gaagctatga atggcatgac tcaagatcag
     3541 attcaattag aagttgttcg tgattaccaa gcctacaatt tagccagtga taaaattaaa
     3601 gtatttgaaa agtcgttaga acaagcaaat gagaacttta gaattactca aaataaattt
     3661 gataatggtt tagaaaccat aacaaatctt ttagaagcag atgccgctca aattactgca
     3721 aatattaatg tgttgaatgc aaaagcagat aaatctctac tttatagaaa gttattacaa
     3781 acaacaggta ttttaattac agaataaaaa taaatttatg gaaaatataa caccacaaag
     3841 tccacaatca gaaaagaaga aaaatatttt atttccaatt attttagcta ttgctattct
     3901 agcaggtgtt ttttttggat ataaagaata tgtacacgga caaacgcatg aaaaaacaga
     3961 caatgcacaa gtaatgagca atatgacccc gattatttct agagtttcag gttatatcaa
     4021 tgaagtaaaa gttacagata atcaatttgt acacaaaggg gatactttat tggttttaga
     4081 caatagagac caaaaaatgt tgctagcaca aactgaagca gcatatataa ccgcacaaaa
     4141 taatatatct gttgccaaat tatcagcaaa tgctgcttca aaaaatatca atacttctaa
     4201 tgcagcagtt aataccgctg atgcacaaat tgatttggca aaagtaaatg tttggaaaac
     4261 aacacaagat tttaatagat atgctaattt gataaaagat cattcgatta cacaacaaca
     4321 atacgaacaa gctgttgctg caaaggaagc tgctgaaaag caattgaaag tattgatgga
     4381 tcaaaaaaac caagtgaaac aacaaactga agtggttgtt tctcaaacgc aaactgccaa
     4441 tcaacaaatt agcactgccg atgcaatgct aaaacaaaga atggtagatg tagaaaatgc
     4501 gaagctaaac ttatcttata ctgtaattac agctcctgaa gatggatttg tttctaaagt
     4561 tccagttcaa aatggacaat taattcaagc aggaatgcaa ctttttagtt taataaagga
     4621 taatgacgta tggatagttg ctaatttcaa agaaactcaa atttctaaaa tgttggaagg
     4681 gcaaaaagta gaaattgaaa tagatgcatt tcctaaacaa aaatttgaag gcattgtaag
     4741 ttcattttcg ccagggacag gttcttcatt ttctgttttg ccaccagata acgcaagtgg
     4801 aaattttgtt aaagtggttc aacgagtacc agttcggatt gattttaaaa atttagataa
     4861 tgcaattgca aaaaaaatgc gtgtaggctt aaatgccaca gtggaagtaa tcttaaagta
     4921 aattctagat gtaattataa gtatggagca agatagttta gtagaatatg gttttcgacg
     4981 tgctattatt actttaacca ctattttatg tgccttattg gagattgtag attctactat
     5041 tgtcaatgtc gcattaaatg aaatgaaagg taatttgggt gctactttat cagaagttgg
     5101 ttgggtagtt actgcctatg caataggaaa tgttattatt gttccaatga caagttggtt
     5161 gtctcaacaa tttgggcgaa gaaattattt tgcagcatca atcattcttt ttactgtttt
     5221 ttcatttatg tgtggaaatg caaccaatat ttgggaattg gtgtttttta gatttatgca
     5281 aggtctagga ggcggtgctt tattggttac ttctcaaact ataattacgg aaacctatcc
     5341 tattgaaaaa cgaggaatgg cgatggcaat ttatggtttg ggtgtgataa ttggtccaac
     5401 cttgggtcca cctttaggag gttatattgt agataattac aattggcctt atatcttcta
     5461 tatcaatatt cccattggaa ttgtagcggc gatacttact ttaagatata taaaaagtcc
     5521 tcgatatgca gaaaaacgtg cagttagaga tattgattgg ctaggtatta ttttattagc
     5581 gatttttgta ggctccttgc aatttgtatt agaacgtgga catgaagatg attggtttga
     5641 tagcacatca attattattt ttacaattac tgcaacttta ggctttatta cttttatttg
     5701 gagagaatta acttataaat acccaatagt cgatttgaga gttttgaaaa atgtcaatct
     5761 aaggataggt acttttatgt catttatttt aggttttggt ttatatggtt ctacatttat
     5821 tattcctatt tatactcaag ggattttagg ttggactgct ctacaagcag gtgccttaat
     5881 gattccatct gcgattacaa ctgcgtttat gatgccaata gttgggaaat taattactaa
     5941 cggtgtaaaa
//
LOCUS       DAOQCW010000134         5694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_6041_length_5694_cov_5.485724, whole genome shotgun sequence.
ACCESSION   DAOQCW010000134 DAOQCW010000000
VERSION     DAOQCW010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6041_length_5694_cov_5.485724"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..760
                     /locus_tag="PLS16_10200"
     CDS             86..760
                     /locus_tag="PLS16_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61496.1"
                     /translation="MKKIKLVVSLLVFSFLSVNAQNNFVVNEIRQIMSRGEQTGFEVP
                     ITGGKPDVVKSNFEKWLKTYKAKVTSSKKTPEIFGDDADIKVVSSNTVDIYATVIPSA
                     AGATVYVFADLGGAFISSSAYPQQYTAMEALLKKFSQDQALQMVETQLSEEEKIAKNL
                     NKEYETLKKDKDSYIKEIEKAKAVIQQRELDIKKNDADQLAKQQQINLQNQIIETVKQ
                     KRNTLK"
     gene            845..1519
                     /locus_tag="PLS16_10205"
     CDS             845..1519
                     /locus_tag="PLS16_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61497.1"
                     /translation="MKKIYLLSFVLVLFLGTTTAQNNFVVNEIRQNMSRGEQTGFEVP
                     IANGKSDIVKANFAKWLKSYKAKVTSSKKSPEVFGDDANIKVVSQNTVDIYANAVPST
                     IGANLYVFVDLGGAFISSSAYPQQYTAMEALLQKFSQSQAVEMLQLQLSDEEKAAKNL
                     DKEYKTLIKGKEKHIKEIEKAKAIIAQRENDINKNNADQSAKLEQINLQKQIIETLKQ
                     KINGLK"
     gene            1605..2825
                     /locus_tag="PLS16_10210"
     CDS             1605..2825
                     /locus_tag="PLS16_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61498.1"
                     /translation="MQQRIVTWGEIGTDLKALITIELDEANANIIINAFPKEKVSKEL
                     QDAIFNEWKNGGNFQFPETTYQWTVDAQQDNILPNNIRVDRPILITEAQSLWSRKLMS
                     AKLNQLLNEEVALLNQQLEGLKEFEQSLWDKAKTQWDKIADYQKKAEISWEQTTILKD
                     KINLIFDSLKALKRINHEGDEEKTLQISKAFNKVIDELEDKLIYPDEWNNIFNELKKI
                     QTNLKESPIKWSAKRKLQNKINDIYAALKKYRSTEFINKSKARIKQLNQILSGFKENI
                     ERDKENYKMQVEKMQHYTRGKLTEAELQERFSHLFDRIKSKEEKIKNISKTINDIERD
                     IQKEQNRQEQELQKAKEQDKQNQEAENNLSDQNYNQNNPTEEIASIVSEDAQPKTTEL
                     EPTTIDNKDNDLTV"
     gene            complement(2869..3624)
                     /locus_tag="PLS16_10215"
     CDS             complement(2869..3624)
                     /locus_tag="PLS16_10215"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HNJ61499.1"
                     /translation="MRKILFQAFLFCVANTSLAQVVNIEAKRFKTDTIGWKGSADLGF
                     SMGKQKETFFMMSANAHVQYKSKKHLYLLLGNSDLVKSKTAKLINSGFIHFRHNFKLK
                     KDWVRWESFTQAQFNKMNGLKVRYLLGTGPRFKILSKEDYRIYIGTLYMYEFEKSLDG
                     LTKLNQVRFSSYLSYTFCPIDVLELIGTFYYQPNITENKDYRLSSENSINVKLHKNLI
                     LGLNFRMNYDSFPPSGAITKLTYLWNNSIKVVF"
     gene            complement(3657..4421)
                     /locus_tag="PLS16_10220"
     CDS             complement(3657..4421)
                     /locus_tag="PLS16_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HNJ61500.1"
                     /translation="MEHYNMNLTLKVWRQKNTKEKGHFETYNVKGISSEMSFLEMFDV
                     LNEDLVHENKEPIAFDHDCREGICGMCSMYINGRPHGPWNATTTCQLHMRAFKDGETI
                     TVEPWRAQAFPVIKDLVVDRSAFDRIIQAGGYISVNTGVTVDANATPISKVNADKAFS
                     TAACIGCGACVAACKNSSAMLFTSAKISQLALLPQGEPERHKRALDMVHQMDEEGFGS
                     CTNTGACEAVCPKEITLESIARMNREFLSASVVSED"
     gene            complement(4472..>5694)
                     /locus_tag="PLS16_10225"
     CDS             complement(4472..>5694)
                     /locus_tag="PLS16_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNJ61501.1"
                     /translation="GAFFANPCYTQIHPTCIPVSGDHQSKLTLMSESLRNDGRIWVPK
                     KKDETRKPNDIPEEERDYYLERRYPSFGNLVPRDVASRAAKERCDAGYGVGTTKQAVY
                     LDFRYNLINKYGRTEASKHNIENPDEATLVRLGKEVVKEQYGNLFDMYQKITGENPYE
                     VPMRIYPAVHYTMGGLWVDYELMTSVKGLYALGECNFSDHGANRLGASALMQGLADGY
                     FVIPYTIGNYLADEISVKPIPTSAPEFEAAEKEVSGRINKLMSIKGSKSVESFHKRLG
                     KIMWEKCGMARNAQGLQEAIQEIKALREEFWNDVRIPGEINEYNPELDKAGRVADFME
                     LGELLCLDALTRNESCGGHFREEYQTEEGEANRDDQSFQHVAAWELKNVGDWTLHKEA
                     LVYENIKIAARSYK"
BASE COUNT         2123 a          924 c          836 g         1811 t
ORIGIN      
        1 gacgagggat attatttggc atttattttg cagtataaag atgattacat tgttaaagtt
       61 ataaaataaa ataaattaaa tgaaaatgaa gaaaattaaa ttggttgtat ctcttttggt
      121 gtttagcttt ttgtccgtaa atgctcaaaa taattttgta gtaaatgaaa tacgacaaat
      181 aatgagtcgt ggagaacaaa caggctttga agtgccaatt actggaggaa aaccagatgt
      241 tgtaaaatcg aattttgaaa aatggttaaa aacttataaa gcaaaagtta catcaagtaa
      301 gaaaacacct gagatctttg gagatgatgc agatatcaaa gtagtaagtt ctaataccgt
      361 agatatttat gccactgtaa ttccaagtgc agctggagca acagtttatg tatttgctga
      421 tttaggtgga gcttttattt cttcttctgc ttatccgcaa caatacactg ctatggaagc
      481 acttttgaag aaattttctc aagatcaggc tttacaaatg gtagaaactc aattatctga
      541 agaagaaaaa atagctaaaa accttaataa agaatatgaa actttaaaga aagataaaga
      601 cagctatatt aaagaaattg aaaaagctaa agcagttatt caacaaagag aattagatat
      661 taagaaaaat gatgctgacc aattggcaaa acagcaacaa ataaacttac agaatcaaat
      721 tattgaaact gtaaaacaaa aaagaaatac tttaaaataa gattaattac tctatcaaat
      781 aagcagtttg taccttgtgc aaactgcttt catatttaaa ataaacaaaa taaataatat
      841 taaaatgaaa aaaatatact tattatcgtt cgtattagtc ctgtttttgg gaactacaac
      901 tgctcaaaat aattttgttg tcaatgaaat acgacaaaat atgagtcgtg gagaacaaac
      961 aggttttgaa gtgccaattg ccaatggcaa atcagatatt gtaaaagcaa actttgctaa
     1021 atggttgaaa tcttataaag ctaaagttac atcaagtaag aaatctcctg aagtttttgg
     1081 tgatgatgcc aatattaaag ttgtaagtca aaacactgtt gatatttatg caaatgcagt
     1141 accaagtact atcggtgcaa atttatacgt atttgtcgat ttgggcggag catttatttc
     1201 ttcttctgca tatccgcagc aatacactgc aatggaagca ttgttgcaaa aattctctca
     1261 aagtcaagca gtagaaatgt tgcaattaca actttcagat gaagaaaaag cagctaaaaa
     1321 cttagataaa gaatacaaaa cactaataaa aggtaaggaa aaacatatca aggaaataga
     1381 aaaagctaaa gcgataattg cacaaagaga aaatgacatc aataaaaata atgctgacca
     1441 aagtgcaaaa ttggaacaaa ttaatttgca aaagcaaata attgaaactt taaaacaaaa
     1501 aataaacggt ttaaaatagt ttatttctat aaaatatagt aatttcgatg agcagtttgt
     1561 acgaaagtac aagctgtttt tactttaacg aaaatattaa aaaaatgcaa caacggattg
     1621 ttacttgggg tgaaattgga acagatttaa aagcactcat aactattgaa cttgacgaag
     1681 cgaacgctaa tattataata aatgcttttc caaaagaaaa agtaagtaaa gagttacaag
     1741 atgccatttt taatgaatgg aagaatgggg ggaatttcca atttcctgaa acgacctatc
     1801 aatggactgt tgatgcacaa caagacaata ttttgccaaa taatattcgt gtagatcgtc
     1861 caattttaat tactgaagct caatctttat ggagtagaaa attaatgagt gccaaattaa
     1921 atcaattatt gaatgaagaa gtagcactct taaatcaaca attagaaggc ttaaaagaat
     1981 ttgagcaatc attatgggat aaagctaaaa cacaatggga taaaattgca gattatcaga
     2041 aaaaagcaga aatttcttgg gaacaaacaa ctattcttaa agataaaatt aatctaattt
     2101 ttgattcctt aaaagcactg aaaagaatta accacgaagg tgatgaggaa aaaacacttc
     2161 aaattagtaa ggcatttaat aaagtaatag atgaattaga agataaatta atttatcctg
     2221 atgagtggaa taatattttt aatgaattaa agaaaatcca aactaacctc aaagaaagcc
     2281 caataaaatg gagtgcaaaa cgtaaacttc aaaataaaat taatgatatt tatgctgcat
     2341 taaaaaaata ccgttctaca gaattcatca ataagtccaa agcaagaatt aagcaactca
     2401 atcaaatatt atctggattt aaggaaaaca tagaacgaga taaggaaaat tataaaatgc
     2461 aagtggagaa aatgcaacac tatactcgag gaaaattgac tgaagctgaa cttcaagaaa
     2521 gatttagtca tctatttgat agaataaaat ccaaagaaga gaaaattaaa aatatctcca
     2581 aaactataaa tgatattgag agagatattc aaaaagagca aaaccgtcaa gaacaggaat
     2641 tacaaaaagc taaagaacaa gataaacaaa atcaagaagc agaaaataat ttaagcgacc
     2701 aaaattataa ccaaaacaat cctactgaag aaattgcttc tattgtttca gaagatgctc
     2761 agccaaaaac aacagaactt gaaccaacaa caatagacaa taaagataat gatttaactg
     2821 tttgatatgt agaagaatta cgctttccca aaatggtgga attgaaaatt aaaaaacaac
     2881 ttttatacta ttattccata aataggttaa tttagtaatt gctccactag gtggaaagct
     2941 gtcataattc attctaaaat tcaaacccaa aattaagttc ttatgtaatt ttacattgat
     3001 tgaattttca gaggagagtc tataatcttt attttcagta atatttggtt gataataaaa
     3061 agtccctatt aattctaaaa catcaatagg acaaaaagta taacttaaat aactactaaa
     3121 tcttacttga tttaatttgg tcaaaccatc aaggcttttt tcaaattcat acatatataa
     3181 agttcctata tatatacgat aatcttcttt acttaaaatt ttaaatctag gtccagttcc
     3241 taataagtaa cgaactttta atccattcat cttattaaat tgtgcctgtg taaaactctc
     3301 ccatcgtacc caatcttttt taagtttgaa attatgtcta aaatgaataa acccagaatt
     3361 gattaatttt gcagttttag atttcactaa atcactattt cccagcaaca aatacaaatg
     3421 ctttttggat ttatattgaa catgggcatt ggcactcatc ataaaaaatg tttctttttg
     3481 cttacccatt gaaaagccta aatctgcact acctttccaa ccaatagtat ctgttttaaa
     3541 tcgtttagct tcaatattaa cgacttgagc taaagaagta ttagcgacac aaaataaaaa
     3601 agcctgaaaa agaatttttc tcatttcagg ctctaagata taaaagtttt ttattattaa
     3661 tcttcagaaa caacacttgc tgacaagaac tctctattca ttcttgcaat gctttctagt
     3721 gtaatctctt taggacaaac agcttcacaa gcacctgtgt ttgtacagct tccgaatcct
     3781 tcttcgtcca tttggtgaac catatctaat gcacgcttat gacgttcagg ttctccttgt
     3841 ggtaataagg ctaattgtga aattttagca gaagtaaata acattgcaga tgagttttta
     3901 caagccgcca cacaagcacc acaaccaata caagccgcag ttgaaaatgc tttatctgca
     3961 tttactttag aaattggagt agcatttgca tcaacagtta cgcccgtatt cacagaaata
     4021 taacctcctg cttgaattat tctatcgaaa gcacttctat ctactaccaa atctttaatc
     4081 accggaaatg cttgagctct ccaaggttca acagtaattg tttcgccatc tttaaatgct
     4141 ctcatgtgta actgacaagt agttgttgca ttccaaggtc catgaggacg tccattaata
     4201 tacattgaac acataccaca aataccctca cgacaatcgt ggtcaaatgc aattggttct
     4261 ttattttcgt gtactaaatc ttcgtttaaa acatcaaaca tttctaagaa tgacatttct
     4321 gaagaaatac ctttaacatt atatgtttca aagtgtcctt tttccttagt gtttttttgt
     4381 ctccacactt tcaaggtaag gttcatatta tagtgttcca taatttattt ttatttgata
     4441 atgagtaaaa ttattaattc gttgaagatg attatttata gcttctagct gcaattttaa
     4501 tattttcata aactaatgct tctttatgta atgtccaatc cccaacattt tttaattccc
     4561 aagctgcaac gtgttggaag ctttgatcat cacgatttgc ttcaccttct tcagtttgat
     4621 attcttctct aaagtgtccg ccgcaacttt catttctagt taaagcatcc aagcataata
     4681 actcacctaa ttccataaag tctgcaacac gtcctgcttt atctaattca ggattgtatt
     4741 catttatttc tccaggaatt ctaacatcat tccaaaattc ttctcttagt gctttaattt
     4801 cttgaattgc ctcttgtaat ccttgagcat tacgagccat accacatttc tcccacataa
     4861 tttttcccaa tcgtttgtgg aaagattcaa cagattttga acctttaata ctcattaatt
     4921 tattgattct acccgacact tctttttcag cagcttcaaa ttcaggggca gatgttggaa
     4981 taggttttac agatatttca tctgctaaat aattaccgat tgtataaggt attacaaaat
     5041 agccatcagc caaaccttgc attaatgcag atgcacctaa acggtttgca ccgtgatctg
     5101 agaaattaca ttctcctaat gcgtacaatc cttttacaga tgtcattaat tcgtaatcta
     5161 cccaaagtcc acccattgta tagtgaacag cagggtaaat tctcataggt acttcgtaag
     5221 gattttcgcc tgtaattttt tggtacatat caaataagtt accatattgc tcttttacaa
     5281 cttctttacc taatctaact aaagttgctt catcaggatt ttcgatatta tgctttgagg
     5341 cttcagttct tccgtattta ttgattaagt tatatctaaa atctaaataa acagcttgtt
     5401 ttgttgtacc tactccataa ccagcatcac atctttcttt agcagcacga gatgctacgt
     5461 ctcttggtac taaattacca aatgaaggat atcttctttc caaataataa tctctctcct
     5521 cttctggaat atcatttggt tttcttgttt catctttttt cttaggcacc catattctac
     5581 catcatttct caatgattcc gacattagtg ttaatttaga ttgatggtct ccagatacag
     5641 gaatacaagt tgggtgaatt tgagtataac aaggattagc aaagaatgct cctt
//
LOCUS       DAOQCW010000135         5479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_6457_length_5479_cov_5.475295, whole genome shotgun sequence.
ACCESSION   DAOQCW010000135 DAOQCW010000000
VERSION     DAOQCW010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5479
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6457_length_5479_cov_5.475295"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..424)
                     /gene="rpsL"
                     /locus_tag="PLS16_10230"
     CDS             complement(17..424)
                     /gene="rpsL"
                     /locus_tag="PLS16_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNJ61502.1"
                     /translation="MPTIQQLVRKGREQLAWTSKSKALTSCPQRRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNKYEVIAYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHIVRGAL
                     DTAGVNNRKKSRSKYGTKKGKAGEAAPVKGKKK"
     gene            complement(733..2031)
                     /gene="tyrS"
                     /locus_tag="PLS16_10235"
     CDS             complement(733..2031)
                     /gene="tyrS"
                     /locus_tag="PLS16_10235"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNJ61503.1"
                     /translation="MRNIVEELSWRGLVHNIIDGTEEQLIKESTTAYIGFDPTSDSLH
                     IGSLVPIILLVHLKNYGHQPIALVGGATGMIGDPSGKSDERNLLTEEILDKNVAGIKR
                     VLSRFLDFNSTDTNAPIMVNNYDWMKDFSFIAFARDVGKRITVNYMMAKDSVKKRFSG
                     DGPGMSFTEFTYQLIQGYDFYHLYKNYNCVLQMGGSDQWGNITTGTELVRRMSDEEAK
                     AYALTCPLVTKADGTKFGKSEGGNIWLDANKTSVYKFYQYWINTSDIDAEKYIKIFTF
                     LDKSTIDILISEHQQAPHLRLLQKRLAEEITTFVHSKEELDKAIEASNILFGKSTKDQ
                     LQKLNNEQIIDVFEGVPTYTIVKEKLATGIGIIDILNGETSILPSKSEARKMIKAGGI
                     QINLEKLDDEQSILNLSDLINEKYIVIRKGKKEYNLIQVE"
     gene            2134..3267
                     /locus_tag="PLS16_10240"
     CDS             2134..3267
                     /locus_tag="PLS16_10240"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNJ61504.1"
                     /translation="MRNLILLLFTFSFGGMLFAEAPKYSNDFMYIGVGANNFGMGNAV
                     VASTNDVTAGYWNPAGLTKIPNKFQMSFMHNEYFASIAKYDYFSFAVPLDSNKHAFGA
                     SFLRFGVDDIPNTLDLLEPDGTINYDNIKSFSVGDYGFMFSYANAMVKNLSVGATAKV
                     IYRKAGKFANAWGFGIDVGAIYTIKDWRIGLTVKDITTTFNAWSYSFTDREKEILQQE
                     ENVIPKNSLEITLPRIQFGAAYTKVFKKAELTAEMDWEITTDGRRNTLIGTNSLSFDP
                     RIGIEAGFFKIFYLRAGVSNFQKMKADGQSKAKMTVQPNLGVGLRFKTIAVDYAYTDV
                     GKASNNQYSHVISAKIGFNKRKAKSSNELIGSPEQPIINENNQ"
     gene            3269..>5479
                     /locus_tag="PLS16_10245"
     CDS             3269..>5479
                     /locus_tag="PLS16_10245"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNJ61505.1"
                     /translation="MKKINLYLFFIVGLIFSLKAQPYGNEWIDFNKTYYKFKVLNKKI
                     CRIDYNTLVNAGISNNLLVGNYFKLFKNGKEVPIYVTTTGQFDNTSFIEFLGDGNDGT
                     FDTNLYKDPNFQPHTAKSLFQDSAVYFLTIDENGPNLRLTPEANDLTAPPTKELFGYR
                     TLSKFVSTIVNGNNAYPQSTNYTDVFESDFGNGEGYGGNLGGGFSQATSFLYNTDTLL
                     TAHVKINYYTNTHVNHNALIKIGSTYSLNDIFYGKSVRKIDVHVPIKNLTNNNTSITV
                     SPTLAGEGAYSSLYSEITYPATFNASNTSVYEFGIDKKDNVYVEIPGFNAQSSNPIVY
                     DLSNNNRYEINNEAILKINLVATTLPKSNIYISAQNTTSITNINNLKAVQFENYSDVS
                     KQGNYIILTNSKAITSSDSINHVELYKQYRNSIAGGTYNTQIYLIQQLDDQFAYGITH
                     HPLAIRNFINFALDHFSEKPEQLFIIGRGRDYRALRNNTDDYIQTYGFPPSDMLLTAR
                     SAIQNTPQIGVGRLDVTDGIQIRDYLEKVKEYEADLNDTIPSAQTPINKLWRKNMIHL
                     GGGYSEMEQLIFKSYLDGYKSTAEAPKYGAKVYSFFKNSSSPLQIAETALVDSLVTNG
                     VSLITFFGHSTTSNVDFNLNPDILHNSGKYNMMFTNGCFVGNLFSGLYGYSHRFVLSP
                     NKGAIGFLGPYDLAYSNILNNYAVQFYNKLCLDNYNAPIGTILKETAKQVIGQPY"
BASE COUNT         1864 a          853 c          811 g         1951 t
ORIGIN      
        1 gaaatatttg aattaattat tttttcttac cttttactgg tgctgcttcg cctgctttac
       61 cttttttagt accgtattta gaacgacttt tctttctatt attaacacct gcagtatcca
      121 aagcacctct aacgatatgg taccttaccc ctggtaaatc tttaaccctt cctcctctaa
      181 ttaaaacaat agagtgctct tgtaagttat gtccttcacc tggaatatat gcgataactt
      241 catatttatt tgttaaacgc acttttgcta ctttacgtaa tgctgaattt ggtttctttg
      301 gtgtagtagt gtacactctt gtacaaaccc ctcttctttg tgggcatgaa gtaagtgctt
      361 tagacttaga tgtccatgct aattgctctc ttccttttct tactaattgt tgaattgtag
      421 gcattcttgc tattttttta tattttggac tgcaaaggta ggcattatcc gcaagaatac
      481 aaaacgtgaa tacaaaaaaa atgtttcttt tttgttattt ttggttttgt agtcttacct
      541 tccgaccgaa gacagtcact acttttacat ttaatcatta ataataactc ttaaatgtgg
      601 tgcaaagata gttaattata taatatagta gtatagttat aaatgttaaa tgcaagttaa
      661 gtttttaatt tcgtcaattt tttatcctat atatctcatt ataaatatga tataatagta
      721 actcctttga aattattcta cttgaattaa attatattct tttttacctt ttctgattac
      781 gatatatttt tcattgatta aatcacttag gtttaatata gattgttcat cgtccaattt
      841 ttctaaattg atttgaattc ctccagcttt tatcattttt ctagcttcag attttgaagg
      901 taaaatacta gtttctccat ttagtatatc aataattcct attcctgtcg ctaatttttc
      961 tttaacaatg gtataagtag gtacaccttc aaaaacatca ataatctgtt cgttatttaa
     1021 tttttgcaac tggtcttttg tagattttcc aaataatata ttagaagctt caatagcttt
     1081 atccaattcc tcttttgaat gcacaaaagt cgttatctct tcagccaatc gtttttgtaa
     1141 taatcttaaa tgtggtgctt gttgatgttc tgatatcaaa atatcaatag ttgatttatc
     1201 taagaaggta aaaatcttaa tatatttttc tgcatcaata tctgaagtat ttatccagta
     1261 ttgataaaat ttatataccg aagttttatt ggcatctaac caaatatttc cgccttctga
     1321 ttttccaaac tttgtaccat cagcttttgt taccaaagga caagttagtg cataagcttt
     1381 agcttcttca tcgctcattc ttctcacaag ttccgtacct gttgtaatat ttccccattg
     1441 gtctgagccg cccatttgta aaacacaatt gtagttttta tataagtggt aaaagtcgta
     1501 accttgaatt aattgataag taaattctgt aaatgacatt ccagggccat ctccagaaaa
     1561 acgttttttc actgaatctt tagccatcat ataatttaca gtaattcttt ttccgacatc
     1621 gcgagcaaat gcaataaaag aaaaatcttt catccaatca tagttattta ccataattgg
     1681 agcatttgta tctgtagaat taaaatctaa aaaacgagaa agcacacgtt ttataccagc
     1741 aacattttta tctaaaattt cttccgttaa taaatttcgt tcatcagatt ttccagatgg
     1801 atcacctatc attccagttg ctccacccac taaagctata ggctgatgtc catagttttt
     1861 cagatgtact aataatataa tcggtactaa acttccaata tgtaaagagt ctgatgttgg
     1921 gtcaaatcca atataagccg tggttgattc ttttatcaac tgttcttctg ttccatcaat
     1981 aatgttatgt actaatcctc tccaacttaa ttcttctaca atatttctca tattatcatt
     2041 ttttttacaa aaattgcatt ttaatttatt aaaaaccttt tactattcaa gtattttggt
     2101 aaatttacaa taatttaaat tattattgaa cttatgcgta atctgatttt gttgctattc
     2161 actttttctt ttggcggaat gctttttgct gaagctccta aatacagtaa tgactttatg
     2221 tatattggtg ttggagcaaa caacttcgga atgggaaatg ctgttgtagc atcaactaat
     2281 gacgttacag caggctattg gaatccagca ggattaacaa aaattcctaa caaatttcaa
     2341 atgtctttta tgcataatga atattttgct agtattgcca aatatgatta tttttctttt
     2401 gcagttccct tagatagtaa caaacatgca tttggagcaa gttttttacg gttcggtgta
     2461 gatgatattc ctaacacttt agatttgtta gaacctgatg gaacgataaa ctatgataat
     2521 attaaatcat tttctgttgg tgattatgga tttatgttta gttatgcaaa tgctatggta
     2581 aaaaatttat ctgtgggagc aactgctaaa gtcatttata gaaaagcagg aaaatttgcc
     2641 aatgcttggg gattcggaat tgatgttgga gcgatttata ccattaaaga ttggcgtatt
     2701 ggattgactg ttaaagatat aacaactact tttaatgctt ggtcttattc atttacagat
     2761 agagaaaaag aaattttaca acaagaagag aatgttattc caaaaaactc attagaaatc
     2821 acacttccta gaatacaatt tggtgctgcc tatactaaag tatttaaaaa agctgaatta
     2881 actgctgaaa tggactggga aattactact gatggaagaa gaaatacgct aatcgggaca
     2941 aatagcttga gttttgaccc acgaattgga attgaagctg gattctttaa aatattttat
     3001 ttaagagcag gagttagtaa tttccaaaaa atgaaagccg atggtcaatc aaaagctaaa
     3061 atgactgtac aaccaaattt gggtgttggt ttgcgattta agaccattgc cgtagattat
     3121 gcttatacag atgtaggaaa agcatctaac aatcaatatt cacatgtaat ttcagctaaa
     3181 attggtttta ataaaagaaa agcaaaaagt agtaacgaac taataggaag ccctgaacaa
     3241 cctattatta atgaaaacaa ccagtaatat gaaaaaaata aacctatatc tattttttat
     3301 tgtaggatta atatttagtc taaaggcaca gccttatgga aatgaatgga tagattttaa
     3361 taaaacgtac tataaattta aagtcttaaa taaaaagatt tgtagaattg attacaatac
     3421 gctagtaaac gctggaatat caaataactt attagttggt aattatttta aactttttaa
     3481 aaatggaaag gaagttccaa tttatgttac aacaactggt caatttgaca ataccagttt
     3541 tatagaattt ttaggtgacg gtaatgatgg aacattcgat accaatttat ataaagatcc
     3601 taattttcaa ccacatactg ctaaaagttt atttcaagat tctgcagtat attttttaac
     3661 gattgatgag aatggtccaa atcttcgatt aacaccagaa gcaaacgact taacagctcc
     3721 accaacaaag gaattatttg gatatagaac tttatcaaaa tttgtatcaa ccatagtaaa
     3781 tggaaataac gcttatcctc aatctactaa ttatacagat gtttttgaat ctgattttgg
     3841 aaacggagaa ggttatggtg gcaatttagg aggcggattt tcacaagcta caagtttttt
     3901 atataatact gacactttgc ttacagccca tgttaaaata aattattata ccaacacaca
     3961 tgttaatcat aatgctttaa taaaaattgg aagcacttat agtctaaatg atatatttta
     4021 tgggaaaagt gtaagaaaaa ttgacgttca tgttccaata aaaaatttaa caaataacaa
     4081 tacaagtatt accgtttctc caacgttggc tggagaagga gcctattctt ctttatattc
     4141 tgaaattacc tatcccgcaa cttttaatgc ttcaaataca tctgtatatg aatttggaat
     4201 tgacaaaaaa gacaatgtat atgttgaaat tccaggtttt aatgctcaaa gtagcaatcc
     4261 tattgtgtat gatttatcca ataataatag gtatgaaata aacaatgaag cgatattaaa
     4321 aattaattta gttgccacga cattacctaa atccaatatt tatatttcag cacaaaatac
     4381 cacttcaatt actaatatta ataatttaaa agctgtacaa tttgaaaatt attcagatgt
     4441 aagcaagcaa ggaaattaca ttattcttac aaattcaaaa gcaattacta gctctgatag
     4501 tattaatcat gttgaactat ataaacaata tagaaattct attgcaggtg gaacatacaa
     4561 tacacaaatc tacctgatac aacaattaga tgatcaattt gcttatggca taacacatca
     4621 tccattagcg attagaaact tcatcaactt tgcattggat cacttttcag aaaagcctga
     4681 acaactattt ataataggaa gaggaagaga ttatagagct ttaagaaata ataccgatga
     4741 ctatatacaa acttatggtt tcccaccatc agatatgtta ttaactgcac gctctgcgat
     4801 tcaaaatacg cctcaaattg gcgtaggaag attagatgta actgatggca ttcaaattag
     4861 agattactta gaaaaagtta aagaatatga ggcagatttg aatgacacaa ttccctctgc
     4921 acaaacgccc attaataaac tttggagaaa aaatatgatt catttaggtg gtggttatag
     4981 tgaaatggaa caattgattt tcaaatccta tttagatgga tataaaagta ccgctgaggc
     5041 tccaaaatac ggtgcaaaag tttatagttt ctttaagaat agttccagtc cacttcaaat
     5101 tgcagaaaca gctttagttg attcattggt tacaaatggc gtttcgttaa ttactttctt
     5161 tgggcattca actacatcaa atgttgactt caacttaaat ccagatatat tgcacaatag
     5221 tggcaaatac aatatgatgt ttaccaatgg ctgttttgtc ggaaatttat tttctggttt
     5281 atatggctat tctcatcgat ttgttttatc tcccaataaa ggagcaattg gttttttagg
     5341 gccttatgat ttggcctatt ctaatattct aaataactat gcagttcagt tttacaataa
     5401 attatgttta gataattata atgcaccaat tggaacaata cttaaagaaa ctgcaaaaca
     5461 agtcattgga cagccttat
//
LOCUS       DAOQCW010000136         5228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_7010_length_5228_cov_5.975449, whole genome shotgun sequence.
ACCESSION   DAOQCW010000136 DAOQCW010000000
VERSION     DAOQCW010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7010_length_5228_cov_5.975449"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            241..2853
                     /locus_tag="PLS16_10250"
     CDS             241..2853
                     /locus_tag="PLS16_10250"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNJ61506.1"
                     /translation="MNLFEILVEKTKEWRDNNYQSDDFPAISEILLFAKEDGKLRFLR
                     EPQYRAMEVYWYLRINQNTPDFITLYKTLFPKRSDFLQALNIDKKQEILQMIMDDEDY
                     LERIKTDDEFVNRNNLESLKESLELSYPSYILALAMGAGKTILIGSIIATEFAMSIEY
                     PEASFMKNALVFAPGTTIIESLKEIADIPFNKIIPPRLYRPFMANIKLTYTKTGEKDI
                     PVEQGGNYNLVVTNTEKIMLRKIRKSATQTQLEFEEKEKQEQLHANLRLQKIASLPNL
                     GIFSDEAHHTYGQKLGEDLKRVRSTINYLHEKKEIICVVNTTGTPYYKSQTLKDVIFW
                     YGIAEGIQDNILKSLENGIRSYSIKKDSESTKEMIEDIIQHFWDKYGAIKTIDGCNSK
                     IAFYFPTEDALLEGRPHIEAALAKIGQPTTIVLKNTQTSKKDEIDSFNALNDPKSIHR
                     VILLVGKGTEGWNCPSLFATALIRKLSGANNFLLQASTRCLRQIANNKLNATIYLDGN
                     NVATLDNELKETYGSSLRELNEKKAEMVTRRLFIIKTEIPKLLIKKVIQKVVQNEEES
                     KPVVFKMPADTKEEKISITTYNPNIVSEAKVLYQSAEGNKTISVNQSIDVYSASLMIA
                     ENYYLDQFDVLKKLKKLYTEEVPHNHLLQLFEQVESQVCNYTITTEEIEEALALIRLT
                     NFDGDNNFKTDEEGRLYTEIRYTKGNDTNFLDKEKTEANTNNFGFHYTPYNFDSGPEK
                     DFYINLLNMINEKPEDIEDIYFTGALTDPNKTDFYFEYKGNDNQFHNYYPDFVIRKKQ
                     KGKKESKFYIVEIKAENERDDSIVGEKGMKKLKMDELEKINKNRFKYEIVFVPGNTVP
                     ADKYNNVKEFLKAE"
     gene            2930..4909
                     /locus_tag="PLS16_10255"
     CDS             2930..4909
                     /locus_tag="PLS16_10255"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNJ61507.1"
                     /translation="METVKSFPSQLVEKFNIEEAPQVPTFESLKNNWTNLLLHGDNRE
                     VLSTLLVNGFQNKVNFVYIDPPFNTGLAYVRKVKLRGTNKKLEGEEMSFDEQIMYENS
                     FLESSFLQFMKDVLVLLSNMLNKDTGLIAVRIDYNYSHYIKVILDEVFSKENFINEIT
                     IGRSREAAGSPSKLEVTTESIYLYSKSNDYKLNPIFAKRPISEIAWTGFTMGGDRNPP
                     ERLFFNKKLLPSSGQHFTLKQEKCDKLIKEHFLRLKCKDCGCLYYYGNSENEFHKQSK
                     KKENRFKYYDITSNTEFKVITDLDKCLNCNTDNFDVEYLGNDDVQINNNWLDIPSYAT
                     TTGYPTENSEELLNRIIRLCAKPGDVILDCFNGSGTTAAVAQKLKMKWIACDINKSSI
                     QTTSNRLQSIIKSQNKIIEKENSKLALEEKEVYYNSFVHYKINDYDLQIQHNELKELV
                     YEHIGIKRMPTDSFFDGTLGTELVKIIPFNHPLSLLDLQLVKTELEKRKDETRNIVLV
                     SLGMEVKVKTAIDDYNKLRPVNKIRTIELRTDKKYGSFFVHKPAQAEVEITKTKDKGK
                     VILKDFISPTIIQRLNIDSKLFQAQIKDFRSQIDVVLIDTDYNGEVFNICISDVPEKK
                     KDFVKGEYEFAIKNKDSKIAVKIIDMLGEEVLIIE"
BASE COUNT         2053 a          804 c          916 g         1455 t
ORIGIN      
        1 ttgtaatgaa ctttagtgct aaaaatccgc cacttcgcca agccgcaaac cgttacaagc
       61 aagccgaaaa aaaaagacaa cgacaggatg aaaaagaatt tttgctgcat aaagacaaag
      121 aagtaaaatg aaaaatgccg acacacaatc cgacacttcg gcttgcaaaa gagctgtttt
      181 cttgctgatg ctcccgacaa aacgattaaa tttggacttt aaaaatttga aagaaattat
      241 atgaatttat ttgaaatatt agtggagaag acaaaggaat ggagagataa taattaccaa
      301 tctgatgact ttcctgctat ttccgagata ttattatttg ctaaagaaga tggcaaattg
      361 cgtttcttga gagaaccgca gtatagagca atggaagttt attggtattt gagaattaat
      421 caaaacactc ctgactttat tactttatac aaaaccttat ttcccaaaag gtctgacttt
      481 ttacaggcgt tgaatattga caaaaagcaa gagattcttc aaatgattat ggatgatgaa
      541 gattatcttg aaagaataaa aaccgatgat gaatttgtaa atcgtaataa tcttgaatca
      601 ttaaaggaat ctttggaatt aagctatcca agttacatac ttgctttagc tatgggtgca
      661 ggtaaaacaa tattgatcgg ctctatcata gcgacagaat ttgcaatgag tatcgaatac
      721 cccgaagcaa gttttatgaa aaatgcttta gtttttgctc caggcacaac aattattgaa
      781 tcactaaaag aaattgcaga cattccgttt aataaaatca tcccccctcg tttgtatcgt
      841 ccatttatgg caaacataaa attgacttac actaaaactg gcgaaaaaga catacctgtt
      901 gagcaaggtg gaaattataa tttggtggta acaaacaccg aaaaaataat gttgcgaaaa
      961 atccgtaaaa gtgcaacgca aacacaactt gaatttgaag aaaaggaaaa gcaagagcaa
     1021 ttacacgcaa atctacgatt acaaaaaatt gcaagtctgc ctaatttggg tatcttttct
     1081 gatgaagcac accataccta cggacagaaa ttgggagaag atttaaaaag agtaagaagc
     1141 accatcaatt atcttcacga aaagaaagaa attatttgcg ttgtaaatac aacaggaaca
     1201 ccttactata aatcccaaac actcaaagat gtaatttttt ggtatgggat tgccgaagga
     1261 atacaagata atattttaaa aagtttagaa aacggcataa gaagttacag cattaaaaag
     1321 gattccgaaa gcaccaaaga aatgattgag gatattattc aacatttttg ggataagtat
     1381 ggtgcaatta aaaccattga cggttgcaat agcaaaattg cgttttattt tccaaccgaa
     1441 gatgctttac tcgaaggacg accacatatt gaagctgcac tcgctaaaat cggtcaaccc
     1501 actacaattg ttttaaaaaa tacgcaaaca tcgaaaaaag atgaaattga cagttttaat
     1561 gctttgaacg acccgaaatc aatacacaga gttattttac ttgttggcaa aggtacagag
     1621 ggatggaact gtcccagttt atttgcaact gcccttattc gcaaactatc gggtgcaaat
     1681 aattttcttc ttcaagcaag tacaagatgt ttacgccaaa ttgccaataa taaactaaat
     1741 gcaacaattt atttagatgg taataatgtt gccacgcttg ataatgaatt gaaagaaact
     1801 tatggtagca gtttgcgaga actcaacgag aagaaagcgg aaatggttac aaggcgatta
     1861 tttattatta agactgaaat ccctaaactt cttatcaaga aagtaattca aaaggttgtt
     1921 caaaatgaag aggaaagcaa acctgttgtt tttaaaatgc ctgctgatac taaagaagaa
     1981 aaaatttcaa taactactta caatccaaat attgtgagcg aagcaaaggt tttataccaa
     2041 tctgccgagg gtaataaaac aatttcagtt aatcaaagta ttgatgttta ttctgcatcg
     2101 cttatgatag ctgaaaatta ttatctcgac caatttgatg ttttgaaaaa gttaaaaaaa
     2161 ctatacacag aagaagttcc acacaaccat ttattacaat tatttgaaca agttgaaagt
     2221 caggtttgta attacacaat aactaccgaa gaaatagaag aagctctggc attaattcgt
     2281 ttgacaaact ttgatggcga caacaatttt aaaacagacg aagaagggcg actatacaca
     2341 gaaatacgat atacaaaagg taatgacact aattttcttg ataaagagaa aacagaagca
     2401 aatacaaaca atttcggctt tcattacact ccttacaatt tcgattcggg tccagaaaaa
     2461 gatttctaca ttaacctgct aaatatgatt aatgaaaaac ccgaagacat tgaagatatt
     2521 tattttactg gtgctttgac cgacccaaat aaaactgatt tttattttga atacaaagga
     2581 aacgacaatc aatttcataa ctattacccc gactttgtga ttcgtaaaaa acaaaaagga
     2641 aaaaaggaaa gcaagtttta catagtggaa attaaagcag aaaatgaaag agatgatagt
     2701 attgttggtg aaaagggaat gaagaagtta aaaatggatg aacttgaaaa gataaacaaa
     2761 aatcggttta agtacgaaat tgtttttgtg cctggcaata cagtgcctgc cgataagtac
     2821 aataatgtta aagaattttt gaaagcagaa taatatggca gagaaaaaag aaatgaaaat
     2881 agcagaagtt aaaggacgac caatgttaca ttggattggc aaacaacctt tggaaacagt
     2941 aaaatcgttc ccttcacaac ttgtcgaaaa atttaatatt gaagaagcac cacaagtgcc
     3001 aacttttgag agtttgaaaa ataattggac aaatctttta cttcacggtg ataacagaga
     3061 agtattgtca acgcttttag taaatggttt tcaaaataaa gtaaatttcg tttacattga
     3121 ccccccattt aatactggct tagcgtatgt tagaaaagtt aaactacgtg ggacaaataa
     3181 aaaacttgaa ggcgaagaaa tgtcttttga tgaacaaata atgtatgaga actccttcct
     3241 tgaaagtagt tttcttcaat ttatgaaaga tgttttagtc cttttgagta atatgcttaa
     3301 taaggatact ggtttaatag cagttagaat tgattacaat tactctcatt acataaaagt
     3361 tattttagat gaggtgtttt caaaagaaaa ttttataaat gaaatcacaa ttggacgaag
     3421 tagagaagca gctggttcac catcaaaatt agaagttaca actgaaagta tttatcttta
     3481 ttcaaaatct aatgactata aattaaatcc tatttttgcg aaaagaccaa tatctgaaat
     3541 tgcttggaca ggttttacaa tgggcggaga tagaaatcca ccagaaagac tattttttaa
     3601 taaaaaatta ttaccctctt caggtcaaca ttttacttta aagcaagaga agtgtgacaa
     3661 actcattaaa gagcattttt tgaggttaaa atgtaaagat tgcggttgtt tgtattatta
     3721 tggtaattca gaaaatgaat ttcataagca atcaaaaaag aaagaaaatc gttttaaata
     3781 ttatgatata acatcaaata cggaatttaa agtaataacc gaccttgata agtgcttaaa
     3841 ctgcaataca gacaattttg atgttgaata tcttggtaat gatgatgttc aaataaataa
     3901 taattggtta gatattcctt catacgcaac gactactggc tatccaacag aaaattcaga
     3961 ggaattatta aataggatta ttcgcttatg tgctaagcca ggtgatgtta ttttagactg
     4021 ctttaatggt agtggcacaa cagcagcagt agcccaaaaa ctaaaaatga aatggattgc
     4081 ttgtgatatt aataaaagtt ctattcaaac aacaagcaac agattacaat caattataaa
     4141 aagtcaaaat aaaataatag aaaaagaaaa cagcaaatta gctttagaag aaaaagaagt
     4201 ttattacaat tcattcgttc attataaaat aaatgattac gatttacaaa ttcagcataa
     4261 tgaattaaag gaattagtat acgagcatat aggaataaaa agaatgccaa cagatagttt
     4321 tttcgatggt actcttggaa ctgaattggt taagattatt cccttcaatc atccgttatc
     4381 attactcgat ttgcagttag taaaaacaga actggaaaaa cgaaaggacg aaacgagaaa
     4441 tattgttttg gtttcgcttg gtatggaggt taaagttaaa acagccattg acgattacaa
     4501 taaactaaga ccagtaaaca aaatcagaac aatagaatta cgcactgata aaaaatatgg
     4561 ttcgtttttc gttcacaaac ctgcacaggc agaagtggaa attacaaaaa caaaagacaa
     4621 agggaaggta atccttaaag actttatttc tccgacaatt attcaacgcc tgaatattga
     4681 cagcaaacta tttcaagcac aaataaaaga tttccgcagt cagattgatg ttgtgcttat
     4741 tgataccgac tataatggcg aagtatttaa catttgcata agcgatgtac cagaaaaaaa
     4801 gaaagatttt gtaaaaggag aatacgaatt tgccatcaaa aacaaggact cgaaaatagc
     4861 agtaaaaata attgatatgt taggtgaaga agtactaata attgaatagc caacacttcg
     4921 tagtaaagca catttgcagg tcgcacaagc cgacacacga ccaaaacctg caaaagagct
     4981 tgcctacccc aacgcacgga caggaaatat gcagcaaaaa tgaaagacag aatgacgaga
     5041 gacaagaacg gccagcttgt aacaggcggt atattttatt gccgagatag tgcaagtttc
     5101 agcgttacta catctaataa actttcgtgt aacttgacag gacagtgctt cgaaatcggc
     5161 aacaaaaaca taccgccaaa ccgttataag caagcctaaa gacgatcgtt catccttcgg
     5221 gcggacac
//
LOCUS       DAOQCW010000137         4919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_7763_length_4919_cov_4.018503, whole genome shotgun sequence.
ACCESSION   DAOQCW010000137 DAOQCW010000000
VERSION     DAOQCW010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7763_length_4919_cov_4.018503"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            280..675
                     /locus_tag="PLS16_10260"
     CDS             280..675
                     /locus_tag="PLS16_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61508.1"
                     /translation="MKIKISLLILLLSVFALHKLDRHHIIFYHCFASGHHTNIDKKGN
                     YYATNQHNAFVRKFTQGGHQIPIDITVDFEKIKEEREKKSDIQDYFISKVVIPLLSFS
                     IMTQYIESISIPLFESVTKKFLLHSVFRI"
     gene            805..1887
                     /locus_tag="PLS16_10265"
     CDS             805..1887
                     /locus_tag="PLS16_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNJ61509.1"
                     /translation="MNRLSLSTVLVMLLLFTSCKNKETAKEEKVQFLATAPLKKDTTL
                     TKDYVAQIHSFQHIELRALEKGYLQNIYVDEGKYVKEGQVMFRIMPRVYEAELAKAKA
                     EVKAAEIEVLNAKTLSDKNIVSKNELLMAQAKLEQAKAEANLASVRLSFTEIRAPFSG
                     YMNHLEARRGSLLDEGDLLTTLSDNSKMWVYFNVPESEYLNIKLNANNDTLMKVNLLM
                     ANGQKFKYNGVVQTIVADFDNETGNIAFRATFPNPDALLRNGETGNIQVTLPFKNALM
                     IPQKATFEVLEKKFVYVIDKNNTVKSREINIAAELPHIFIIKSGLEETDKILLEGLRQ
                     VHENDKIEYKFEKPESVISNLNLYAE"
     gene            1911..>4919
                     /locus_tag="PLS16_10270"
     CDS             1911..>4919
                     /locus_tag="PLS16_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNJ61510.1"
                     /translation="MFSKFIQRPVLAIVISVVIVFVGVLAIEKMPISQFPDIAPTTVN
                     ISIAYPGSSADVLVKSSLITLEQAINGVQDMRYMATDATSAGEATLRIIFEPGTDPND
                     AVIRVKTRVDQVMPLLPELVQREGVVIMPIQPSMLMYVNLYSKNKSMDEKFLFNYATV
                     KMIPEIQRTKGIARAQILGSRRYAMRVWLNPERMRAYNISVEDVMDAIGEQSIIGRPG
                     RIGQSSGIAAQSLEYVLTYKGRYNTPEEYEDIIIRAKSNGESIHLRDIAKVELGSEFF
                     DIYSNLDGHPSAAIVLKQNYGSNASDVIKEVKEKLDEMKKDFPPGMDYKISYDVSQFL
                     DASIEQVLETLRDAFILVALVVFVFLGDWRSTLIPILAVPVSLVGAFFVIQFFGLTIN
                     LVTLFALVLAIGIVVDNAIVVVEAVHAKMEEDPEISSYKAVKKVLAEIGGAIIAITMV
                     MVSVFIPISFMSGPVGTFYRQFSITMASSIVISAITALTLTPVLCAMLLKNQHGKPKK
                     KGLLTRALDAFNGRFEILTEKYVNLLKRIVSRRMVTFVVLILFCVGVYFGNKFVPSGF
                     IPNEDQGTIYAIIQTPPGSTLEQTNQVSQRLQKICEKVEGVESVSSLAGYEIMTEGRG
                     SNAGTCLINLKSWSERSHTVKEIMEELEEKSKGIGAIIEFFEPPAIPGFGASGGFSMR
                     LLNKTTNTDYHDFDKVNKEFMANLGKRKELTGLFTFFAANYPQYELEIDNKMAMQKGV
                     TIGKAMENLNIMIGSTYEQGFIKYNQFFKVYVQSDPQFRRLPSDILNLFVKNEAGEMV
                     PYSAFMKLKKGQGPNEITRFNMYNSAAIQGLPAKGYTTADAIAAIREVAKTTLPNGYD
                     IAFDGLSYDEAMRGNESLYIFLIVLLFVYFVLAAQYESFSIPMAIILSLPVGIFGSFL
                     VLKLMGLENNIYAQVGMIMLVGLLGKNAVLIVEFAVQKRTQGASIFDAAVEGAKVRFR
                     PILMTSFAFIAGLIPLVIAKGAGAIGNRTIG"
BASE COUNT         1696 a          672 c          922 g         1629 t
ORIGIN      
        1 aataaaaaat ctgtctaaag gaatttattt attgaatttg aacaatgaag atggtatagc
       61 aacttatcaa ctattggtgc aataataaat cgaattacaa tctcgcaata acaaaaagca
      121 actcttgggt tgcttttttg tttaattaaa attaattact tgaaacaatt ctttagtaat
      181 atatagatat tgttaagtaa aatttatgta ttgcttactt aacagatttt aataaattag
      241 taatgtttaa aatcatatcc attatataaa tttggattcg tgaaaattaa aatatcacta
      301 ctaattttac ttttatctgt ttttgctcta cacaagctgg acagacacca tataatattt
      361 tatcattgtt ttgcttctgg tcatcatacc aatattgaca aaaaaggaaa ttattatgca
      421 actaatcagc ataatgcatt cgtaaggaaa tttactcaag gaggacatca aattcccatt
      481 gatattaccg ttgattttga aaaaataaaa gaggaaagag agaaaaaatc tgatattcaa
      541 gattatttta ttagtaaagt ggttattcca ttattatctt tctcaataat gactcaatat
      601 attgaaagta tttcaattcc attgtttgag tcagttacta agaaattctt attacacagc
      661 gttttccgaa tttgatttta aaatagctaa tttttatcag actacatagt ctgatactta
      721 agttgtttat acctctatca aattaattta actctaatca atcaaagtcg caagacacaa
      781 ttttttaaaa attattttat ttatatgaac agattatcat tatctactgt gctagtaatg
      841 ctattactat ttaccagttg taaaaacaaa gaaactgcaa aagaagaaaa agtacagttt
      901 ttagctaccg ctccattaaa aaaggatact actttaacca aggattatgt tgcacaaata
      961 cactctttcc aacatattga gttacgtgca ttagagaaag gttatctaca aaatatttat
     1021 gtggacgaag gaaaatatgt aaaagaagga caagtaatgt ttcgaataat gcctagggtt
     1081 tatgaggcag aattagctaa agccaaagca gaagttaaag cagctgaaat tgaggtgctt
     1141 aatgcgaaga ctttatcaga taaaaatatc gtttctaaaa atgaattatt aatggcacaa
     1201 gcaaaactag aacaagccaa agcggaagca aatttagcta gtgttcgtct ctcttttact
     1261 gaaattagag cacctttcag tggctatatg aatcatttgg aagccagacg aggaagtttg
     1321 ttagatgaag gagatttatt aactacatta tctgacaaca gtaaaatgtg ggtttacttt
     1381 aatgttccag aaagtgaata cttgaatatc aaattaaatg caaataatga tactttaatg
     1441 aaagtgaatt tattaatggc aaatggccaa aaatttaagt ataatggcgt agtacaaacc
     1501 attgtcgctg attttgataa tgaaactggt aatattgcat ttcgtgctac atttcctaat
     1561 ccagatgcct tgttgagaaa tggggaaaca ggcaatatac aagttacttt gccatttaag
     1621 aatgcactaa tgattcctca gaaagcaaca tttgaagtat tagaaaagaa atttgtatat
     1681 gttatagata aaaacaatac cgttaaatca agagagataa atatagcagc tgagttaccg
     1741 catattttta taataaaaag tggtttagaa gaaacagata aaatattgtt agaaggattg
     1801 cgtcaggttc atgaaaacga taaaattgaa tataagtttg aaaaaccaga atctgtgatt
     1861 tctaatttga atttatatgc tgaataatta aatcctgaaa agtaaaagtt atgtttagta
     1921 aatttataca aagaccggta ttagctattg ttatatctgt tgttatagtt tttgtaggtg
     1981 ttttggcaat tgaaaaaatg ccaatttctc aattccctga tattgcaccg acaacggtta
     2041 atatatcaat tgcttatcca ggttcaagtg ctgatgtttt agtaaaatca tcacttatta
     2101 cactagaaca agccattaat ggtgtacaag atatgcgtta tatggctact gatgcgacaa
     2161 gtgcaggtga agctacatta agaattattt ttgaacctgg cacagacccc aacgatgcag
     2221 taattagagt gaagactagg gttgatcaag taatgccttt attgcccgag ttagtacaac
     2281 gtgaaggcgt agttattatg ccaattcagc cgagtatgtt gatgtacgtt aacctttatt
     2341 cgaagaataa gagtatggat gaaaaattct tattcaatta tgccacagta aaaatgatac
     2401 ctgaaattca acgtacaaaa ggtattgcaa gagctcaaat tttgggtagc cgaagatatg
     2461 caatgcgtgt ttggttgaat ccagagcgta tgcgagctta taatatttct gttgaagatg
     2521 tgatggatgc tattggagaa caaagtatta ttggacgtcc aggaagaata ggacaaagtt
     2581 caggaattgc tgcacagtct ttagagtatg tgcttacata caaaggacgt tataatacac
     2641 ctgaggaata tgaggatatt ataattcgtg cgaaatctaa tggagaaagc attcatttaa
     2701 gagatattgc taaagtagaa ttgggcagcg agttttttga tatttattct aatcttgatg
     2761 gacatccatc tgctgctatc gttttaaaac aaaattatgg tagtaatgca agcgatgtaa
     2821 ttaaggaagt taaagagaaa ctcgatgaga tgaaaaaaga ctttcctccc ggtatggatt
     2881 ataaaatcag ttatgatgta tcacaattct tagatgcgtc aatagagcag gttctggaaa
     2941 ctttaagaga tgcatttatt ttagtagcat tggttgtttt tgtgttttta ggcgattggc
     3001 gttctacatt aatcccaatt cttgcagtgc cagtatctct agttggtgca ttttttgtga
     3061 ttcaattttt tggacttaca attaacttgg ttacactctt tgcattggta ttagcaattg
     3121 gtatagttgt agataatgca atagtagtgg tcgaggcagt gcatgctaag atggaagaag
     3181 acccagaaat ttcttcctat aaagcagtta aaaaagtact tgctgaaatt ggaggagcaa
     3241 ttattgcaat cactatggta atggtttctg tatttattcc tatttcattt atgtctggtc
     3301 cggttggaac attttatcga caattttcga ttacaatggc gagttctatt gttatttcag
     3361 caattacggc acttacactt acgcctgtat tgtgtgcaat gctattgaaa aaccaacatg
     3421 gaaagccaaa gaaaaaaggt ttgctgacaa gagctcttga tgctttcaat ggaagatttg
     3481 aaatccttac tgaaaaatat gtgaatttgt tgaagcgaat tgtaagtaga agaatggtaa
     3541 cgtttgttgt tttaattcta ttctgtgtgg gtgtttattt tggaaataaa tttgtgccat
     3601 ctggttttat tccaaatgaa gaccaaggta ctatttatgc tattattcag acacctccag
     3661 gttcgacatt ggaacaaacc aaccaagtat ctcagagact tcaaaaaatc tgtgaaaaag
     3721 tagaaggtgt tgagtcggta tcttctttag ctggatatga gattatgacc gaaggacgtg
     3781 gctctaacgc aggtacttgt ttgattaatc taaaatcatg gtcagaacgt tctcatactg
     3841 tgaaagagat tatggaagaa ttagaagaga aatcaaaagg tattggtgca attattgaat
     3901 tttttgaacc acctgcaatt ccaggcttcg gagcatcagg tggtttttca atgcgtttat
     3961 taaataaaac gaccaataca gattaccatg attttgataa agtaaataaa gaatttatgg
     4021 caaatttagg taagcgaaaa gaattgacgg gcttgtttac tttctttgct gcaaattatc
     4081 cacagtatga attagaaata gataataaaa tggcgatgca aaaaggcgta actattggga
     4141 aagcaatgga aaatctcaat attatgatag gtagtactta tgagcaaggt tttattaagt
     4201 acaaccaatt tttcaaagtg tatgtgcaat cggatccaca atttagaaga cttccatctg
     4261 atattttaaa tctatttgtt aaaaatgaag ctggagaaat ggtgccttat tctgctttta
     4321 tgaaactaaa aaaaggacag ggtccaaacg aaattactcg ttttaatatg tataattcag
     4381 cagctattca aggtcttcct gcaaaaggat atacaacagc agatgctatc gcagctataa
     4441 gagaagtggc aaagacgaca cttccaaatg gttatgatat cgcctttgac ggattgtctt
     4501 atgatgaagc tatgagaggt aatgaatcgc tttatatatt cctaattgtg ttgctgtttg
     4561 tttactttgt attagctgct caatatgaaa gtttctcaat tcctatggca ataatactct
     4621 ctttgcctgt gggtattttt ggttcatttt tagttcttaa attgatgggt ttagaaaata
     4681 atatttacgc tcaagtgggt atgattatgt tagtagggtt attgggcaaa aatgcggtat
     4741 tgattgtaga atttgctgtt cagaaaagaa ctcaaggagc aagtatattt gatgctgcag
     4801 ttgaaggtgc taaggttcgt tttcgtccaa ttttaatgac ttcttttgca tttattgcag
     4861 gattaatccc acttgttatt gcaaaaggag caggagcaat tggtaacaga actattggt
//
LOCUS       DAOQCW010000138         4862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_7912_length_4862_cov_5.208238, whole genome shotgun sequence.
ACCESSION   DAOQCW010000138 DAOQCW010000000
VERSION     DAOQCW010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7912_length_4862_cov_5.208238"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /gene="rplB"
                     /locus_tag="PLS16_10275"
     CDS             complement(<1..317)
                     /gene="rplB"
                     /locus_tag="PLS16_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584594.1"
                     /note="one of the primary rRNA-binding proteins; required
                     for association of the 30S and 50S subunits to form the
                     70S ribosome, for tRNA binding and peptide bond formation;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNJ61511.1"
                     /translation="MATKKFTPITPGLRFKVANAYTEITTDKPEKSLIAPIKRSGGRN
                     QKGAMTMRYIGGGHKKQYRIIDFKRDKADVPATVKTVEYDPNRSAFIALLFYKDGEKR
                     YIIA"
     gene            complement(339..629)
                     /gene="rplW"
                     /locus_tag="PLS16_10280"
     CDS             complement(339..629)
                     /gene="rplW"
                     /locus_tag="PLS16_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNJ61512.1"
                     /translation="MGILKKPLITEKSQKLQDSSKKYTFIVDKKANKVAIKRAIEEMY
                     GVNVAVVNTNTMPSKPKSKYTKSNIISGRRGSYKKAVISLSAGEEIDFYTDL"
     gene            complement(633..1265)
                     /gene="rplD"
                     /locus_tag="PLS16_10285"
     CDS             complement(633..1265)
                     /gene="rplD"
                     /locus_tag="PLS16_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNJ61513.1"
                     /translation="MKVDVYNKLGQSTGRQVELPDSIFAIEPNEHVIYLAVKQYLANR
                     RSGTHKTLEKSEISGSTRKLHKQKGTGGSRKGSIKNPLFRGGARIFGPRPRNYGFKLN
                     KKVKELAKYSALSAKVASNSIKVIEDFSMEAPKTKEYINLLKGFGLENKKSLLIMPEL
                     NDNIYLSSRNIQGAGLSDIASLNTYDIMNNSMILLSEKSVEALSNQIENN"
     gene            complement(1304..1921)
                     /gene="rplC"
                     /locus_tag="PLS16_10290"
     CDS             complement(1304..1921)
                     /gene="rplC"
                     /locus_tag="PLS16_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNJ61514.1"
                     /translation="MKGLIGTKIGMTSIFGDQGQIIPCTVIEAGPCVVTQIKSKAKDG
                     YDAVQIGYGTKKEKNTTKALKGHFEKAQTAPKSKLVEFRGTFDVELGAVVSTDILKEG
                     DKISISANSKGKGFQGVVKRHNFSGVGGQTHGQHNRLRSPGSIGACSTPSRVFKGMKM
                     GGRMGNETVKLPGSKIIKIIPEQNYIIVKGSVPGHNGAIVVLEKK"
     gene            complement(1927..2232)
                     /gene="rpsJ"
                     /locus_tag="PLS16_10295"
     CDS             complement(1927..2232)
                     /gene="rpsJ"
                     /locus_tag="PLS16_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNJ61515.1"
                     /translation="MTQRIRIKLKSYDHNLIDKSAEKIVKTVKNAGAVVTGPIPLPTN
                     QKIFTVLRSPHVNKTAREQYRLNTYKRLLDIYSATPKTVDALMKLELPSGVDVEIKG"
     gene            complement(2237..4342)
                     /gene="fusA"
                     /locus_tag="PLS16_10300"
     CDS             complement(2237..4342)
                     /gene="fusA"
                     /locus_tag="PLS16_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNJ61516.1"
                     /translation="MARDLKYTRNIGIAAHIDAGKTTTTERILFYSGVNHKLGETHEG
                     SATTDWMAQEQERGITITSAAVTIGWKYRNDNYHVNIIDTPGHVDFTVEVNRSLRVLD
                     GLVFLFSAVDGVEPQSETNWRLANNYNVARLGFVNKMDRQGADFLNVVGQVKSMLGSN
                     AVPLQLPIGAEDDFKGVVDLINFRGMVWNTEDYGMTYEVVDIPADMLEEATEYREKLL
                     EAVSEYDEKLMEKYFEDPSSITEREILDALRAATIDGSIVPMVCGSSFRNKGVQTMLD
                     LVMEILPSPLDKENINGTDPKTDAPISRKPSYDEPFTALAFKIATDPYVGRLAFTRAY
                     AGVLEAGSYVLNTRSGNKERISRIFQMHANKQNPIERLGAGDIGAVVGFKDIRTGDTL
                     CDEKAPIILESMKFPDPVIGLAIEPKTQKDADKLGMALGKLAEEDPTFKVRYDEDTAQ
                     TVISGMGELHLEIIVDRLLREFKVECNQGAPQVNYKETITQSVNHREKYAKQSGGRGK
                     FADIAVEVGPGAEDKEGLEFINDIFGGAVPREFIPSVEKGFKEAMKTGVLAGYEVPSL
                     KVRLHDGSFHQVDSDSFSFEQAAKFAFREACRKANPILLEPIMKLEVITPEENTGDVV
                     GDLNRRRGMMEGMEMKNNANVIKAKVPLSEMFGYVTDLRTITSGRATSIMEFSHYAQA
                     PNNITEQVIAKVKSKVKAD"
     gene            complement(4379..4846)
                     /gene="rpsG"
                     /locus_tag="PLS16_10305"
     CDS             complement(4379..4846)
                     /gene="rpsG"
                     /locus_tag="PLS16_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNJ61517.1"
                     /translation="MRKQRAKKRYLAPDPIYNDTVVTRFVNTIMLDGKKSIAYTVFYE
                     AMDKIKASTGEEGYDIWKKAMDNVMPAIEVRSRRIGGATFQIPTPIREDRKMFLMMKW
                     LVNFARKRNGKSMGDKLAAELIAASKGEGSSVKRKEDMHKMAEANKAFSHFKV"
BASE COUNT         1455 a          946 c          606 g         1855 t
ORIGIN      
        1 gcaataatat atcttttttc tccatcttta taaaataata aagcgatgaa tgccgatcta
       61 tttggatcat attcaactgt ttttacagtt gcaggaacat cagctttatc tcttttaaaa
      121 tcaataattc tatattgttt tttatgacca cctccgatat aacgcatagt catcgcacct
      181 ttttgatttc taccgccact tcttttgata ggagctatca aagatttttc tggtttatca
      241 gtagtaattt ccgtatatgc attcgcaacc ttaaagcgaa gacctggagt tattggtgta
      301 aatttttttg ttgccatctc ttgttattct ttcttatctt ataaatcagt ataaaaatca
      361 atttcttcgc cagcagataa agaaataact gcttttttat agctacctct tctaccagaa
      421 ataatattac ttttagtata tttagatttt ggttttgatg gcattgtatt cgtattcact
      481 acagctacat tcactccata catttcttct attgctcttt taatcgctac tttattcgct
      541 tttttatcaa caataaaagt atattttttt gaagaatctt gaagtttctg agatttctcc
      601 gttattaaag gtttctttaa aattcccatc tcttaattgt tttctatttg gttagacaat
      661 gcttccactg atttctcact caataatatc atactattat tcatgatatc ataagtattt
      721 aaacttgcaa tatcagataa accagcacct tgaatatttc tagatgataa gtagatatta
      781 tcatttaatt caggcataat taataatgac tttttgtttt ctaatccaaa accttttaat
      841 aaattgatat attctttagt tttaggagct tccattgaaa aatcttcgat tactttaata
      901 gaattagaag caacttttgc agataaagca gaatattttg ctaattcttt aaccttttta
      961 ttcaatttaa aaccatagtt tctaggtctt ggtccaaaaa ttcttgcacc acctctaaat
     1021 aatggatttt tgatactacc ttttcttgaa cctccagttc ctttttgttt atgtaacttt
     1081 ctagtagaac ctgaaatttc agatttttct aaagttttgt gcgtaccact tcttctatta
     1141 gctaagtatt gttttacagc taaatagata acgtgctcat ttggttcaat tgcaaaaatt
     1201 gaatctggga gctctacttg gcgtccagtg gattgaccta atttattata tacatctact
     1261 ttcatctctt cttatgtaat ttgcttatta aacaaataga tttttatttc ttttctaata
     1321 ctacaattgc accattatgt ccaggaacag aaccttttac tataatataa ttttgttcag
     1381 gaataatttt gataatttta gaacctggta atttaacagt ttcatttccc attctaccac
     1441 ccattttcat acctttaaaa actcttgaag gcgttgaaca agctccgata gaaccaggag
     1501 atcttaatct attatgctga ccatgagttt gtccaccaac tccactaaag ttatgtcttt
     1561 ttacaacacc ttgaaatcct tttcctttag aattggcact aatactaatt ttatcacctt
     1621 cttttaatat atctgtagaa actactgctc ctaattcaac atcaaaagta cctctaaatt
     1681 caactaattt agatttaggt gcagtttgtg ctttttcaaa atgacctttt aaagctttag
     1741 ttgtattttt ttctttttta gtaccatatc ctatttgcac tgcatcatag ccatctttag
     1801 cttttgattt aatttgagta acaacacaag gtccagcctc aataacagta caagggataa
     1861 tttgaccttg atctccaaag atactagtca ttcctatttt agttcctatt aatcctttca
     1921 tttcaattaa ccttttattt ctacatcaac accacttggt aattctaatt tcattagagc
     1981 atcaacagtt ttaggtgtag cactataaat atctaacaat ctcttataag tatttaatct
     2041 atattgttct ctagcagttt tattaacgtg tggagaacgt aatactgtaa atattttttg
     2101 gtttgtaggc aaaggaattg gtcctgtaac tactgcacca gcatttttaa ctgttttaac
     2161 gattttttct gctgatttat caatcagatt atgatcgtaa gattttaatt ttattctgat
     2221 acgttgtgtc ataatattaa tctgctttta ctttactttt aacttttgca attacttgtt
     2281 ctgtaatatt gtttggagct tgtgcataat gcgagaactc cataatagat gtagctcttc
     2341 ctgatgtaat tgttcttaaa tcagtaacat aaccaaacat ttcactcaaa ggaactttag
     2401 ctttaattac gttagcatta tttttcattt ccataccttc catcatacct cttcttctgt
     2461 tcaaatctcc tacaacatca cctgtatttt cttctggagt aataacttcc agtttcataa
     2521 taggttctaa taaaatagga ttagcttttc tacatgcttc tctaaaagca aatttagcag
     2581 cttgctcaaa agagaatgaa tctgaatcca cttggtggaa tgaaccatca tgtaatctta
     2641 cttttaaaga aggtacttca taacctgcaa gaacacccgt tttcatagct tctttaaatc
     2701 ctttttctac agaagggata aattctctag gtacagcacc accgaaaata tcattaataa
     2761 attctaaacc ttctttatct tcagcacctg gtccaacctc aacagcgata tcagcaaatt
     2821 tacctctacc accagattgt ttagcatatt tctctctgtg atttacagac tgcgtaattg
     2881 tttctttata atttacttga ggagcacctt gattacattc cactttgaac tctcgtaata
     2941 atctatctac gatgatttct aagtgtaatt cacccatacc actaattaca gtttgagcag
     3001 tatcctcatc atatctaact ttaaaagttg gatcttcttc agctaatttt cccaaagcca
     3061 tacctaattt atcagcatct ttttgagttt taggctcaat tgccaaacca attactggat
     3121 caggaaattt catagattct aatatgattg gagctttctc atcacataaa gtatctccag
     3181 ttctaatatc tttaaaacct acaactgctc caatatctcc tgcacctaat ctttcaattg
     3241 gattttgttt atttgcatgc atttggaaaa tacgagagat acgttcttta tttccacttc
     3301 ttgtatttaa tacataagaa ccagcttcta atacaccagc ataagctcta gtaaatgcta
     3361 aacgtccaac ataagggtca gtagcaattt taaatgctaa agcagtgaaa ggctcatcat
     3421 aacttggttt tctagaaatt ggagcatctg tctttggatc agttccattg atattttcct
     3481 tatctaatgg acttggtaat atttccatta ctaaatccaa catagtttgt acccctttat
     3541 ttctaaaaga tgaaccacaa accataggaa caatagaacc atcaattgta gctgctctta
     3601 atgcatccaa aatttctctt tctgtgatag aagatggatc ttcaaaatat ttttccatca
     3661 atttttcatc gtattcagaa acagcttcta ataatttttc tctatattca gtcgcttctt
     3721 caagcatatc agctggaata tcaacaactt cataagtcat accataatcc tctgtattcc
     3781 aaaccattcc tcggaagtta attaaatcaa ctacaccttt gaaatcatcc tctgcaccaa
     3841 ttggtaattg taatggaact gcattacttc ctaacattga ttttacttga ccaacaacat
     3901 ttaagaagtc agcaccttgt ctatccattt tattaacgaa gcccaaacga gcaacattat
     3961 agttattagc taaacgccag ttagtttcag attgaggctc aacaccatca acagcagaga
     4021 ataagaacac taaaccatct aacactctta atgaacgatt tacctcaact gtgaagtcaa
     4081 cgtgacctgg tgtatcaata atgtttacat ggtaattatc atttctatat ttccaaccaa
     4141 tagttacagc agcagacgta atagtaatac ctctttcttg ctcttgtgcc atccagtcag
     4201 ttgttgcaga accttcgtga gtttctccaa gtttatgatt cacaccagaa tagaaaagaa
     4261 tacgttctgt tgtagttgtc ttacctgcat caatgtgtgc tgcaatacca atgtttcttg
     4321 tatattttaa atcgcgagcc atttaatctt tcgtattatt ttttctttta tttttaaatt
     4381 aaactttaaa gtgagagaat gccttattag cttcagccat tttatgcata tcttcttttc
     4441 tttttacaga tgaaccttca cctttagaag ctgctattaa ttcagctgct aatttatcac
     4501 ccattgattt tccgtttctt tttcttgcaa aattcaccaa ccacttcatc attaagaaca
     4561 tttttctatc ttctctgatt ggtgttggaa tttgaaatgt tgcaccgccg attcttctac
     4621 ttctaacttc aatcgctggc attacattat ccatagcttt tttccagata tcataacctt
     4681 cctctccagt gcttgcttta attttatcca ttgcttcata aaaaactgta taagcgatag
     4741 atttttttcc atccaacata atagtattca caaaacgtgt aactaccgta tcattataga
     4801 ttggatccgg tgctaaatat cttttctttg ctctttgctt tctcatctta tattatagaa
     4861 at
//
LOCUS       DAOQCW010000139         4849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_7947_length_4849_cov_6.433041, whole genome shotgun sequence.
ACCESSION   DAOQCW010000139 DAOQCW010000000
VERSION     DAOQCW010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7947_length_4849_cov_6.433041"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PLS16_10310"
     CDS             complement(<1..314)
                     /locus_tag="PLS16_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HNJ61518.1"
                     /translation="MKILLIRFSSIGDIVLTSPIIRCLKTQLAQVEIHYLTKNIYTEI
                     LKANPYIDKIHSIQKNTSEIIDLLKKEQYDVCIDLHNNIRSNQIKSALKIKNTYTFNK
                     QS"
     gene            complement(366..719)
                     /locus_tag="PLS16_10315"
     CDS             complement(366..719)
                     /locus_tag="PLS16_10315"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNJ61519.1"
                     /translation="MNKLTSNQKIGINGEAVALNFLKNLNYTILHTNWRHKHAEIDII
                     AQDKNCIVFIEVKTRNSNKFGYPEEFVTANKIKKMHEAADAFLEQTNWQNELRFDIIA
                     IENYARITHFIDAFY"
     gene            828..1820
                     /locus_tag="PLS16_10320"
     CDS             828..1820
                     /locus_tag="PLS16_10320"
                     /inference="COORDINATES: protein motif:HMM:NF006426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HNJ61520.1"
                     /translation="MHQLVDEFPNQLRRAIDIGQKAKIGKPKFPIRNVLITGLGGSGI
                     GGTIISNILRDDLQVPVVINKEYRIPAFVNENTLVIVSSYSGNTEETLSAMMQAFKKD
                     AQIVCITSGGLVKEYADTNDFDYVLIDGGAPPRAAFGQSFVQIFFILHYLGLLQNDFI
                     NYLEKSIALLEEDKADIKKVAYDIASKLNGKIPVIYSDAKYEGVAVRFRQQINENSKM
                     LCWHHVIPEMNHNELVGWREKNENIAVVFLKNAQDYERNQERMEFVKTVVSQYASTTI
                     EIESKGEKDIERVLFLIHLTDWVSCYLADLKGVDAIEVDVISQLKNKLAENPII"
     gene            1822..2508
                     /gene="lipB"
                     /locus_tag="PLS16_10325"
     CDS             1822..2508
                     /gene="lipB"
                     /locus_tag="PLS16_10325"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNJ61521.1"
                     /translation="MQQIQLHDVGRIRYADALTLQEEFFNHLVEQKLAGISNENQHHL
                     ILCEHFPVITVGKAGKDSNILLPETLLHEKGVDVFHINRGGDVTFHGFGQITGYPILD
                     LDFFTSDLKHYMWLLEEVMIQTISSYNIEGYRIDGATGVWVNSKIDKQPKKIAAFGVK
                     TSRWVTMHGFSLNVDVDLNYFNLINPCGFTDKGVTSIKEETNADINIEEIKQLILKNF
                     SKIFNISFYL"
     gene            2618..3664
                     /locus_tag="PLS16_10330"
     CDS             2618..3664
                     /locus_tag="PLS16_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61522.1"
                     /translation="MKTITKYTVLLLITILLFSCGKDKGGNPTKKEEEKTVSIYAIGN
                     SKTKIKLWKDSTSQTITDGTYAAEAFTIIISGDDVYVSGSEKNSNNKSVAKIWKNGTA
                     QALTDLTSNSKDAAAYDIKISGSDVYAVGYESGLDNKAKLWKNGTVETLTGGTAGHVS
                     SKANAITLSGSDVYVAGNLAKSGKSVAGIWKNGALKLLTDGKRNAEANAIAVSGSDVY
                     VAGNSENGNGSVRVAVLWKNGKAQNLTEGKGSETAGAHAVAISGTDVYVAGFERKTNG
                     AIVAILWKNGKAQELTNDSSNSNDARAYTIAISGKDVYVAGVRNGNAIIWKNGKALTL
                     VDNKAASIYDLFIK"
     gene            complement(3934..>4849)
                     /locus_tag="PLS16_10335"
     CDS             complement(3934..>4849)
                     /locus_tag="PLS16_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61523.1"
                     /translation="IFDFVKRVNLRAVNKKNIESLSQAGAFDSFEDLHRAHYNHQFND
                     GTTFIEKLIKYGNHSRDIESQNQNSLFGGTDDAGIPEPDIPPANKWSNFEQLQKEKEV
                     TGIYISGHPLDDFNLEISHVCNTDFEGLDEFKTKDVIIACMVGSINKRVDKNGKPWAI
                     VTFQDVMGSQEVLFFNKDYINFLAYLEVGTLVVLRGKYIPSFRDKSIFELRINSIEYL
                     HDLMEKSAKSLNIFMHYASLNEKFVDEIYGVLQKHKGKKPFKVYLLDDNKNEETLSFA
                     NKKFGVNITKDLYNELDEIRNIIVKLTF"
BASE COUNT         1738 a          636 c          811 g         1664 t
ORIGIN      
        1 atactttgtt tgttgaaagt ataagtattc ttaatcttta acgcagattt tatttgattg
       61 gaacgtatgt tattgtgtaa atcaatacat acatcatact gttctttttt aagtaagtca
      121 ataatttcag atgtgttttt ttgaatacta tgaattttat ctatgtatgg attggctttt
      181 aatatttcgg tataaatatt tttagttaaa taatgaattt ctacttgagc aagttgtgtt
      241 tttaaacatc gaataatagg agaggtaagg acaatgtcac caatggaact aaatctaata
      301 aggagaattt tcatttttat ttttggaaat ttttgggaac taaaatacaa tgtttaaata
      361 gtaaatcaat aaaatgcatc aataaaatga gtgattctcg catagttttc aatagcaata
      421 atgtcaaatc ttaattcatt ttgccaattg gtttgttcta aaaaggcatc agcagcttcg
      481 tgcatttttt tgattttatt agctgttaca aactcttcag gatagccaaa cttattagaa
      541 tttcttgttt ttacttctat aaatacaata caatttttat cctgagcgat aatgtcaatt
      601 tctgcatgtt tatgtctcca attggtatgc aaaatcgtat aatttagatt tttaagaaaa
      661 tttagggcga cagcttctcc atttatccca attttttgat ttgatgttaa tttgttcaag
      721 ataaaaaatt tgttttgcag attaaattta attattttgt ggcatatatt ctgatgattt
      781 tttattagaa tagtgtttaa taaaataaaa taagtaaaat gataacaatg catcaattag
      841 tagatgaatt tccgaaccaa cttcgtagag ccatagatat aggtcaaaaa gcaaaaattg
      901 gcaaaccaaa attcccaatt agaaatgtat taattacagg attaggcggt tctggaattg
      961 gcggtacaat tatttcaaat attttaagag atgatttaca agtgcctgtt gttattaata
     1021 aagaatatag aatccctgca ttcgtaaatg aaaatacttt ggtcattgta tcttcctatt
     1081 caggaaatac agaagagact ttaagtgcga tgatgcaggc atttaaaaaa gatgcacaga
     1141 ttgtttgtat tacatcaggc ggtttggtaa aagaatatgc cgatacaaat gattttgatt
     1201 atgttttaat tgatggtggc gctccgccac gtgccgcatt tgggcaatca tttgtacaaa
     1261 tatttttcat attgcactac ttaggattat tgcaaaacga ttttataaat tatcttgaaa
     1321 agtcaatagc tttattagaa gaagataaag ctgatattaa aaaggtagct tatgatatag
     1381 caagtaaatt aaatggtaaa attcctgtta tttattcaga tgcaaaatat gaaggggttg
     1441 ccgtgcgatt tagacaacaa atcaatgaaa attctaagat gctgtgttgg catcatgtga
     1501 taccagaaat gaaccacaat gaattggtgg gttggagaga aaagaatgaa aatattgctg
     1561 tagttttctt gaaaaatgct caagattatg agcgtaacca agaaagaatg gagtttgtaa
     1621 aaacagtggt aagtcaatat gcttcaacta cgatagaaat tgaatctaag ggtgaaaaag
     1681 atatagaaag agtacttttc ttaattcatc ttaccgattg ggtttcttgt tatttagcag
     1741 atttaaaagg tgttgatgct attgaagtag atgttatttc acagttgaaa aacaaattag
     1801 cagaaaatcc tatcatataa aatgcaacaa attcaactgc atgatgtagg gagaattcgg
     1861 tatgctgatg ctttaacact tcaagaagaa tttttcaatc atttagttga acaaaagtta
     1921 gctggaatca gtaatgaaaa ccaacatcat ctgattttat gtgaacattt tcctgttatt
     1981 acagtaggaa aagcagggaa agattctaat attttattgc cagaaacttt attacacgaa
     2041 aaaggtgttg atgtttttca tatcaatcgt ggaggcgatg taacctttca tggatttgga
     2101 caaattactg gttatccaat tttagattta gatttcttca cttcagattt aaaacattat
     2161 atgtggcttc ttgaagaagt tatgatacaa actatttcaa gctacaatat agaaggttat
     2221 cgaattgatg gtgctactgg tgtttgggta aattccaaga tagataaaca accaaaaaag
     2281 attgcagctt ttggtgtaaa aacttctcgt tgggtaacaa tgcatggttt ttctttgaat
     2341 gttgatgtgg atttgaacta ttttaattta attaatcctt gtggatttac ggacaaaggc
     2401 gttacgtcta tcaaagaaga aacaaatgct gacataaata ttgaagaaat aaagcaactt
     2461 attctcaaaa attttagcaa aatatttaac attagttttt atttgtaata atagcttctt
     2521 ttaattcttt ttaaaagata attctctata ttaattttta aaatctattt acaaattatt
     2581 tttttattag taatttcagt cttataaaat cataataatg aaaacaataa caaaatacac
     2641 agtattatta ttaataacaa tattactatt ttcttgtggc aaagacaaag gtggaaatcc
     2701 aacaaagaaa gaagaagaga aaactgtttc tatctacgca ataggcaata gcaaaactaa
     2761 aattaaacta tggaaagata gtacttctca aactattaca gatggaactt atgctgctga
     2821 agcttttacc attataatat caggagatga tgtttatgtt tcaggtagcg aaaaaaatag
     2881 caacaacaaa tcagttgcaa aaatttggaa aaatgggacg gctcaagctc ttactgattt
     2941 gacatcaaat tctaaagatg cagcagctta tgacattaaa atatcaggca gtgatgttta
     3001 tgcagtcgga tatgaatctg gattagacaa taaggcaaaa ctatggaaaa atggaactgt
     3061 agaaacttta actggcggaa cagcaggaca cgtaagttct aaggcaaatg ccattacttt
     3121 atctggaagc gatgtttatg tagctggaaa tttagctaaa tctggaaaat ctgtagccgg
     3181 aatttggaaa aatggagcat taaaattatt aactgatggc aaacgaaatg cagaggcgaa
     3241 tgctattgca gtatctggaa gtgatgttta tgtagctgga aattcagaaa atggaaatgg
     3301 ctctgttcgt gtggctgttt tatggaaaaa tggaaaagct cagaatctta cagaaggaaa
     3361 aggctctgaa acagcaggag ctcatgccgt agcaatttca ggaactgatg tatatgtagc
     3421 tggttttgaa agaaaaacca atggggctat agtcgcaata ctttggaaaa atggcaaagc
     3481 tcaagaactt acaaatgata gtagcaattc aaatgatgcg agagcatata ctattgccat
     3541 atctggaaaa gatgtttatg tagctggagt tagaaatgga aacgcaatta tttggaaaaa
     3601 tggaaaggca ttgactttag ttgataataa agcagcatct atctatgatt tgtttatcaa
     3661 ataaatagtt caagaaaaat aaatctctta atttagaaga gcaaaatatt taacgttagt
     3721 tttttttgat ttttatttgt aataagtcta aataaactta tctttgcttc atcatgcaaa
     3781 agcagataga agaaaagaaa attacattag agcaaacaca tctttctgat gatggtattt
     3841 gcagtttgca aaaattttta atgcaggctt gttgcaaaaa atctaaatgc tgcgaaaaat
     3901 ataaaagaaa aggaaagcat tgtaaaaaat gtcctaaaaa gtaagtttta caattatatt
     3961 tcttatttca tctaattcat tgtacaagtc ttttgtaata tttacaccaa attttttatt
     4021 ggcaaacgat agcgtttctt catttttatt atcatctaat aaatagacct taaagggttt
     4081 tttgcctttg tgtttttgta gtacaccata aatttcatca acaaattttt catttaaaga
     4141 agcataatgc ataaaaatat ttagtgattt tgcacttttt tccatcaaat catgtaaata
     4201 ttctatacta ttaattctaa gttcaaaaat tgatttatct ctaaaacttg gtatatattt
     4261 acctcttaaa acgactagtg ttccaacttc taaataagcc aaaaagttta tataatcttt
     4321 attgaaaaat aaaacttctt gtgaacccat aacatcttga aaagtaacaa tagcccaagg
     4381 ttttccgttt ttatcaactc ttttgttgat gcttccaacc atgcatgcaa taattacgtc
     4441 tttcgtttta aactcatcaa gtccttcaaa gtcagtattg caaacatgac tgatttctaa
     4501 gttaaaatca tcgagcggat gcccagaaat gtatatacca gttacttctt tttccttttg
     4561 caattgttca aaattagacc atttattggc aggtggaata tctggctctg gaatgccagc
     4621 atcatcagtg ccaccaaata atgaattctg attttgagat tcaatatctc ttgaatgatt
     4681 cccgtattta atgagttttt caatgaaagt agtaccatca ttaaattgat gattataatg
     4741 tgctctgtgt aaatcttcaa aactatcaaa agctccagct tgtgataaag attcaatatt
     4801 ttttttatta acggctctaa gatttacacg ttttacaaaa tcaaagata
//
LOCUS       DAOQCW010000140         4821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_8015_length_4821_cov_5.310533, whole genome shotgun sequence.
ACCESSION   DAOQCW010000140 DAOQCW010000000
VERSION     DAOQCW010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8015_length_4821_cov_5.310533"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PLS16_10340"
     CDS             complement(<1..211)
                     /locus_tag="PLS16_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61524.1"
                     /translation="MAKKTATSAPAKKAAAPAKKAAAKAPAKKAAAPAKKAAAKAPAK
                     KAAAPAKKAAAKAPAKKAAAPAKKAA"
     gene            697..1674
                     /locus_tag="PLS16_10345"
     CDS             697..1674
                     /locus_tag="PLS16_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61525.1"
                     /translation="MKNIVYIFLLAIVLSIAYTSCKRNKSDVGEIVEDSTMVAYELLP
                     TEQQDVINLVYSWNRAHDTNAIYGLDTIYNETVLFYKKTFSKRNIIETKKNRIKRADS
                     YKQRIIGRIGIYPSDTGDYKCDFLKLVTIDGKPKVYHTYLVFQRFNDVWKVIVESDKE
                     SDAQPELANLFTDFKESESTSEGDFDGDGEKEELIVIKPPQDSIGKYLSTTTKISFTN
                     FNIPEIDIENCIGVNIMNENDVDGDGADDFSIVIKKPDGSYGSIILYSYKRGNWKQLA
                     KFKATQDDITQNRKELIEFAGSGNIKIRVAEKLPDNRDTTLIKTISTWD"
     gene            1722..2957
                     /locus_tag="PLS16_10350"
     CDS             1722..2957
                     /locus_tag="PLS16_10350"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNJ61526.1"
                     /translation="MIEFSKIVLENELKVIHHYDASTPFIIVNVLYHIGAKDEDENRT
                     GFAHLFEHLMFGGTKLFPNFDHPLQDAGGENNAFTNNDYTNYYDVIPKENIETALCLE
                     ADRMLDLDINEQSLKVQKDVVCEEFKEHYINQPYGDAWHKLRALVYEQHPYKWPTIGK
                     NLKQIEEATLQDVRDFYAAYYSPSNAILVISGNIEKENAFSLTEKYFGHLKSKTVQHK
                     AFSEPEQQVAKTQTTYSDVPLNAIFIAFKITDKKNKDYYTADTITDILSTGTSSRLYQ
                     NLIKKEKAFVEIDAYITGSNDVGMFVIEGKIAEDCSVERAESLIWNELEQLQKELISA
                     KELQKIKNKMLTYMAFSESSLMNRTVALAHFELLGDANQINQEIDAYEKVSAEAIQTF
                     ANAYLIKSRSNTLYYLKQQ"
     gene            2959..4218
                     /locus_tag="PLS16_10355"
     CDS             2959..4218
                     /locus_tag="PLS16_10355"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNJ61527.1"
                     /translation="MNIPPIQAIGAIQFTLPKQLQLSNQIPVYIFNDDKNPILKLDIV
                     FDAGRWTEEQKLAADAMAALSKSGTKNLSSFEINEAIDQLGATLKIGTGYNTCTLSIS
                     CLSKFLEPCLELALTCLTDIVFPENELQIFKKNTKAKLLVNNEKTDYLSGVIFRETIF
                     GKEHPYGYRTNNERIEQIDRNEIIRYYQSNLLASNCTVFIAGDVNENCLNQLESVLSR
                     FKNTSVNNFKPQHIIQTDSNLKIREKKAKSTQASLTMGKILFNKQNKDYGNFILLNTI
                     FGGYFGSRLMSNIREDKGLTYGIYSMLNPLKHDGFWAIYTDTNIDKLDICIQEIEIEL
                     DRLQNELISENEIQLARNYLLGRFLKRTDGAFNLMETFKSYIIEGVDIQHFGTFIEDI
                     KNADAKLLQNLAQKYLSLNSMHQVIVG"
     gene            4220..>4821
                     /locus_tag="PLS16_10360"
     CDS             4220..>4821
                     /locus_tag="PLS16_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61528.1"
                     /translation="MKFNIKKYFFVCLLIAQSIGYAQNNLFEAPNLYCVRNFTSTSIV
                     LAWQKPAVASCFVAYDVYYKPENGNFSLLFSLNNSNKKDTTINHPNPGQTGYFYMTQR
                     GSCNSSSTTKIYSDTLDSRLSFPSVEIKKVSVVNNQIELQWIPDAYKEIKGYLIYSNK
                     TTNNQFNTPIDTVFGNMVGTYIDTKVNVNDSQYSYKIRTL"
BASE COUNT         1807 a          610 c          791 g         1613 t
ORIGIN      
        1 ctgctgcttt tttagctgga gctgctgctt tttttgcagg tgcttttgct gctgcctttt
       61 tagctggagc cgctgctttt tttgcaggtg ctttcgctgc tgctttttta gctggagctg
      121 ctgctttttt tgcaggtgct tttgctgctg cttttttagc tggtgcagcc gcttttttag
      181 ccggagccga tgttgctgtt ttctttgcca ttgtatgact tttaattttt gtttcatttg
      241 gttacagctg aaacgattac tgtagatctt tttatttttt aataggttga aaaaatcttt
      301 taaaattcct acaatataaa agtaaaacat aatttatata taatcctatt ttctgcttgt
      361 aacaacttga tgcacgatga tttgcaatgt gaataagtat aggtataaaa tcaataaaga
      421 tagatttatt tattgagtac aaaataaatg gagaattgta tgttcgacgt tataaccata
      481 ataaaataag gcatcttaga aagttatatt gcaaagtgaa tactaaagta ttttattata
      541 tgaggctgta acacttgcaa aaatggtgct tacagcaaat tctattcata aaaaaatgtg
      601 gatatttttt taaaaaaata aaaataacat actttaattc aaataaggat aagatattaa
      661 ttctatctat gtaattttgt aacattaaaa ttaaagatga aaaacatagt ttatattttc
      721 ttgttagcaa ttgtattaag tattgcttat acatcttgta agcgtaataa aagtgatgta
      781 ggagaaatag tagaagactc cactatggtg gcttatgaac tattacctac agaacagcaa
      841 gacgttatta atttagtata tagttggaat cgtgcacatg atacaaatgc tatctatggt
      901 ttggatacta tttataatga aacggtgttg ttttataaaa aaacatttag caagcgaaat
      961 attattgaaa ctaaaaaaaa tagaattaaa agagcagact cttataagca aagaataatt
     1021 ggacgcattg gaatttatcc atcagatacg ggtgattata aatgtgattt cttgaaatta
     1081 gttactatag atggcaaacc taaagtatat catacttatt tagtatttca gcgatttaat
     1141 gatgtttgga aagttattgt agaaagcgat aaagaatctg atgcacaacc tgaacttgcg
     1201 aatttattta cagattttaa agaaagcgaa tcgacttcag aaggcgattt tgatggtgat
     1261 ggtgaaaaag aagaattgat tgttattaaa ccacctcaag attctatagg aaaatattta
     1321 tcaacaacaa ctaaaatttc ttttaccaat tttaatattc cagagattga tattgagaat
     1381 tgcattggtg taaatattat gaatgaaaat gacgtcgatg gagatggtgc agatgatttt
     1441 tctattgtga taaaaaaacc tgatggctct tatggtagta ttattttata tagttataaa
     1501 agaggtaatt ggaaacagct tgcaaagttt aaagccactc aagatgatat tacacaaaat
     1561 agaaaagaat taattgagtt tgcaggaagt ggaaatatta aaattagagt tgctgaaaaa
     1621 ttacctgaca atagagatac gactttaata aaaacaatat ctacttggga ttaaaatgag
     1681 tttagattac aacaattgta atctgcaatt aaaaaaaata tatgattgaa ttttcaaaaa
     1741 tagtattaga aaacgaactt aaagttattc atcattatga tgcatcaaca ccatttatta
     1801 ttgtaaatgt attgtatcat attggagcaa aagatgaaga tgaaaacaga actggttttg
     1861 ctcatttatt tgaacacttg atgtttggcg gaacaaaatt atttccaaat tttgatcatc
     1921 ctttacaaga tgctggaggc gaaaataatg catttacaaa taatgactat accaattatt
     1981 atgatgtaat tcctaaagaa aatatagaga cggcactttg tttagaagcc gacagaatgc
     2041 tagatttgga tattaatgag caatctttaa aagtccaaaa agatgttgta tgcgaggaat
     2101 ttaaagaaca ttatattaat caaccttatg gcgatgcttg gcataagctt cgagctttgg
     2161 tttatgagca acatccatat aaatggccaa caattggaaa aaatttaaag caaattgaag
     2221 aggcaacatt gcaagatgtt cgagattttt atgccgccta ttactcgcca agtaatgcaa
     2281 tattagtaat ttctgggaat attgaaaaag aaaatgcgtt ctctttaaca gaaaaatatt
     2341 ttggacacct aaaatcaaaa actgttcaac ataaagcttt ttctgagcca gagcaacaag
     2401 ttgcaaaaac acaaacaact tatagtgatg ttccattgaa tgctattttt attgcattta
     2461 aaataacaga caagaaaaac aaagattact atactgctga tactataaca gatattttat
     2521 ctactggtac atcttcaaga ttgtatcaaa atttgataaa aaaagaaaaa gcatttgttg
     2581 aaattgatgc atatattaca ggaagtaatg atgttggtat gtttgtaatt gaaggaaaaa
     2641 ttgctgaaga ttgttctgtt gaacgagcgg aatctttaat ttggaatgaa ttagaacaat
     2701 tacaaaaaga attaatcagt gctaaggaat tacagaaaat aaaaaataaa atgttgacct
     2761 atatggcatt ttctgagagt agtttaatga acagaactgt agcattggca cactttgaac
     2821 tattaggcga tgccaatcaa atcaatcagg aaattgatgc ttatgaaaag gttagtgctg
     2881 aagcgataca aacttttgca aatgcatatt taattaaatc tagatctaat actttgtatt
     2941 atctaaaaca acaataaaat gaatattcca cctatacaag ctatcggagc aatacaattt
     3001 acgctaccaa aacaattaca attaagcaat caaattccag tctatatttt taatgatgat
     3061 aaaaatccta ttttaaaatt agatattgtt tttgatgctg ggagatggac agaagaacaa
     3121 aaattagcag cagatgctat ggctgcttta tcaaaaagcg gtactaaaaa tttaagttct
     3181 tttgaaatta atgaagcaat cgaccaactt ggagcaacct taaaaattgg aacaggttat
     3241 aatacttgta cactttccat atcttgtttg agtaagtttt tagaaccttg tttggaattg
     3301 gctttaacat gtttaacaga cattgttttt ccagaaaatg aattgcaaat ttttaagaaa
     3361 aatacgaaag ccaaattatt agtaaataac gaaaaaaccg attatttatc tggcgtaatt
     3421 ttcagagaaa ccatatttgg gaaagagcat ccgtatggct atcgaacgaa taatgaacgt
     3481 attgaacaaa ttgatagaaa tgaaattata cgttattacc aaagtaattt actagcttca
     3541 aattgtaccg tttttattgc tggagatgta aatgaaaact gtttaaatca attagaaagc
     3601 gttttatcta ggtttaaaaa tacgagtgtt aataatttta aaccacaaca tataattcaa
     3661 acagacagta atttaaaaat aagagaaaag aaagccaaaa gtactcaagc ttctttgact
     3721 atggggaaaa ttttatttaa taaacaaaat aaagattatg gcaattttat tttattgaat
     3781 acaatttttg ggggttattt tggttcaaga ttaatgagta atatccgaga agataaaggg
     3841 ttgacgtatg gtatttattc tatgctgaat cctttaaaac atgatggatt ttgggcaatt
     3901 tatacagaca ccaatattga taaattagat atttgtattc aagaaattga aatcgaatta
     3961 gatagattac aaaatgaatt aatttcagaa aatgaaattc aactagcaag aaattattta
     4021 ttaggacgat ttctgaagcg aacagatgga gcctttaacc tgatggaaac atttaaaagc
     4081 tatataattg aaggtgttga tattcagcat tttggcacat ttattgaaga tataaaaaat
     4141 gctgatgcta aactattaca gaatttggct caaaaatatt tatctttaaa ttcaatgcat
     4201 caagtaattg ttggataata tgaaattcaa tatcaaaaaa tatttttttg tatgtttgtt
     4261 aattgctcaa tcaattgggt atgctcaaaa taatttattt gaagctccta atttatattg
     4321 tgtccgcaat tttacttcaa cttctattgt attggcgtgg caaaaacctg cagtcgctag
     4381 ttgttttgtt gcttatgatg tgtattacaa acctgaaaac ggcaatttta gtttgttgtt
     4441 ttcattgaat aattctaaca aaaaagacac aaccatcaat catccaaacc caggacaaac
     4501 aggttatttt tatatgaccc aacgtggaag ttgcaatagc tcttcaacaa caaaaatata
     4561 ttctgataca ttagatagcc gactttcttt tccatctgta gaaattaaaa aagtgtctgt
     4621 tgttaataat caaattgaat tacaatggat tccagatgca tataaagaaa taaaagggta
     4681 cttaatttat tctaataaaa ctactaataa tcaatttaac acaccaattg atacagtatt
     4741 tggaaatatg gttggaactt atatcgacac aaaagtaaat gtaaacgact ctcaatattc
     4801 ctataaaatt agaactttaa t
//
LOCUS       DAOQCW010000141         4794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_8096_length_4794_cov_5.479004, whole genome shotgun sequence.
ACCESSION   DAOQCW010000141 DAOQCW010000000
VERSION     DAOQCW010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8096_length_4794_cov_5.479004"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1592)
                     /locus_tag="PLS16_10365"
     CDS             complement(<1..1592)
                     /locus_tag="PLS16_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HNJ61529.1"
                     /translation="MSKTTRIYIVAFTALIVLLCVYYLSFTFKVNSIEKNVAKIAEQS
                     VKMEAPEKRYPGNRLLQYLYQDTIRQEQINFKNRYLDSIQNEKVFLGVTYKKAKEQQL
                     NLGLDLQGGMSVVLQVSLRELLTSMSDNSKDPNFIKALDQAEKQMQTSQDDYITLFAK
                     AFKEINPNGKLASLFATRQYADRINFNTSDKDVLKVIREESTSAVQRTYSIITSRIDQ
                     FGTKQPTVSLDLGRGRISVEIAGVDNPARVRKLLQATADLEFWETYKTNEIGNSLNKA
                     NDALKVVLDNEKSGDKVSSPLATNDNILTQGTTETKSNTTEVKDTAKDILGNLANKAK
                     TDTSQEAKMEEYKNENPLFSVMYPSVDNNNRYTESPIIGYVRALDTATVNRYLSYPEV
                     LDAFPRDIKFMYGMKPVGKEDDPNNKAKVYALYAIKMGSTDGEAPLDGAAITNSKKDI
                     DQSGQVVVSMSMNHEGARTWSDLTKRNIGNFIAIAVDNQIVSAPVVNTQIDGGNSVIQ
                     GGFTQEEAQDLSNMLKIGKLPAS"
     gene            complement(1744..3114)
                     /locus_tag="PLS16_10370"
     CDS             complement(1744..3114)
                     /locus_tag="PLS16_10370"
                     /inference="COORDINATES: protein motif:HMM:NF013275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNJ61530.1"
                     /translation="MISVQNHLQLLSGNNKIVLFLLVIGMFTSCAAGKKSGSSNGANV
                     TEIVEPNIVKDTIKKEVIPFDVIEEIKDSVKSEIKEEVKPITSQITPEILKDKYRISV
                     VLPFILKQIPLSGVYVNDTTKQLLPDSKKAMDFYLGCKLAYEEISNYSKKLNVYFLDD
                     NNNPTDLIGLLDGSIFDKSDYIIAPFDNEQVKMLAEFGKVNQKIVLSPTVNSIYITRD
                     NPYYYSVNPTLNSQYQFILDKIKDATPNTTIEVVYSSTDSISENISLLKNIIAQNIKS
                     GSNVNIQYYDLISTTNVAQQLYSKDTISKRVVLLYSSNLNYIKPILNQMGSIKNPLDI
                     YTNSSVRYSKNLVTAKNFKQRIYCPAAYDANNDQTKLFKQKYIDKFVKEPTELSYLGY
                     DLMKLLFYKIDKNETLLQNSKSIYNEYLQQDFNFVPINNSKGELQYYDNAYLNMLHFS
                     NGIFVQ"
     gene            complement(3071..4612)
                     /gene="guaA"
                     /locus_tag="PLS16_10375"
     CDS             complement(3071..4612)
                     /gene="guaA"
                     /locus_tag="PLS16_10375"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNJ61531.1"
                     /translation="MQHNKILIFDFGSQFTQLIARRIRELNVYCEIVPYNTKFQDLMS
                     ENIKGIILSGSPFSVLQDNALEFPILDFIKAYNLPILGVCYGAQYLAKTMNGTVEKSS
                     KREYGRANINILQGADLLKDIPQKSQVWMSHGDSIIQLPDDFELLASTDDIPVAAFKW
                     KNKNVFGIQFHPEVTHSIDGKTLLKNFVVDICGCQQDWSELSFIEETVQELKQTIGSD
                     KVVLGLSGGVDSSVAALLLHKAIGQQLYCIFVNNGLLRYNEYDSVLESYKQLGLNIIG
                     VDASNDFYSELAGTSEPEAKRKIIGKVFIDIFQREANKLENVTYLAQGTIYPDVIESV
                     SVHGPSAKIKSHHNVGGLPEKMHLKIVEPLRKLFKDEVRRVGKALELPENILNRHPFP
                     GPGLGIRILGDITKEKVEMLQLADDIFISSLKEDNLYNDVWQAGVILLPIQSVGVMGD
                     ERTYEQVVALRAVSSVDGMTADWCHLPYEFLAKVSNKIINNVRGINRVVYDISSKPPA
                     TIEWE"
BASE COUNT         1586 a          817 c          609 g         1782 t
ORIGIN      
        1 gatgctggca atttaccaat tttcaacata tttgataaat cttgtgcctc ttcttgagta
       61 aatccgcctt gaattactga atttccacca tcaatttgtg tattaacaac tggtgctgaa
      121 acaatttgat tatcaacagc gattgcaata aaattaccta tatttctttt agtcaaatca
      181 ctccaagttc tagcaccttc gtggttcatt gacatactta caacaacttg tccggattgg
      241 tctatatctt ttttagagtt tgttatcgca gcaccatcta atggagcttc accatcggtt
      301 gaacccattt ttatagcata taaggcataa actttagctt tattatttgg gtcatcttct
      361 ttcccaactg gtttcatacc atacatgaat tttatatcac gagggaatgc atctaaaact
      421 tcaggataac ttaaatatct atttacagtt gctgtatcta atgctcttac ataaccaatt
      481 ataggacttt cagtatatct attattatta tctacggaag gatacattac actaaataat
      541 ggattttcat ttttatattc ttccattttt gcttcttgcg aagtatccgt ttttgcttta
      601 ttagctaaat ttcctaaaat atcttttgca gtatctttaa cttcagttgt attggatttt
      661 gtttcagtag tgccttgcgt taaaatatta tcatttgtcg ctaatggaga acttacttta
      721 tcgccagatt tttcattatc taaaactact tttaaagcat cattagcttt gtttaaagag
      781 tttccaattt catttgtttt ataggtttcc caaaactcta aatctgcagt agcttgcaat
      841 aattttctaa cacgtgctgg attatcaaca cctgcaattt ctactgaaat acggcctctt
      901 cctaaatcta aacttacagt tggttgtttc gttccaaatt ggtcaattct agaagtaatg
      961 atactataag ttctttgaac agcagaagtt gattcttctc ttattacttt taaaacgtct
     1021 ttatcgctag tattaaaatt aattctatca gcatattgac gagttgcaaa taaagaagct
     1081 aattttccat ttggattaat ttctttaaaa gctttagcaa ataatgtaat ataatcatct
     1141 tgtgaagttt gcatttgctt ttcagcttga tctaaagctt taataaaatt tgggtcttta
     1201 gaattatcag acatagaagt caataattca cgaagcgaaa cttgcaacac aacactcatt
     1261 ccaccttgta aatctaaacc taaattcaat tgttgttctt tggctttttt gtacgttaca
     1321 cctaagaata ctttttcatt ttggatagaa tccaagtatc tatttttaaa attaatttgt
     1381 tcttgacgga tcgtatcttg atacaaatat tgcaataatc tatttcctgg atagcgtttc
     1441 tctggtgctt ccatttttac tgattgttca gcaatttttg caacattttt ttcaattgaa
     1501 tttaccttaa atgtaaagga taggtaataa acacaaagta atacaataag tgcagtaaag
     1561 gcaacaatat aaattctggt agttttactc atgatatttt tttaaacaag gtgcaaatat
     1621 actattttaa cgtgaaaaaa acagaaaatt cccaaatatt aaaaatatgt tattatccac
     1681 agataactta ttcaaaacca aagtgttgat tattggagtg aattagcaat tataaaaaat
     1741 gaattactgg acaaaaatac cattagaaaa atgtaacata ttcagataag cattatcgta
     1801 atattgcaac tctccttttg aattatttat cggtacaaaa ttaaaatctt gctgtaaata
     1861 ttcattgtat atagactttg aattttgtaa caatgtttca tttttatcta ttttatagaa
     1921 taataacttc ataaggtcat agcccaaata agacaattct gtaggttctt taacaaattt
     1981 atctatatat ttttgtttaa acaatttggt ttgatcatta tttgcgtcat aagcagctgg
     2041 acaataaata cgttgcttaa agttttttgc tgtaaccaaa tttttactgt aacgaacaga
     2101 agaattagta taaatatcta aaggattctt tatacttccc atttgattaa gtataggttt
     2161 tatatagttt aagttgctag aatacaacaa aacaactctt ttactaatgg tatctttgga
     2221 atacaattgt tgagcaacat ttgtagttga aatcaaatca tagtattgga tatttacatt
     2281 agaaccactt ttaatatttt gagcaataat atttttcagt aaactaatat tttcagaaat
     2341 agaatctgtg gaactataaa cgacttcaat tgtagtgttt ggagttgcat ctttaatttt
     2401 atccaaaata aactgatatt ggctattcaa agttggattg acactataat aataaggatt
     2461 atctcttgtt atataaatag aattaaccgt aggtgataaa actatttttt gattgacttt
     2521 tccaaactct gccaacattt ttacttgttc attatcaaaa ggagcaatga tataatctga
     2581 tttatcaaaa atacttccat ctaataatcc aattaaatca gttggattgt tattatcatc
     2641 tagaaaataa acatttagtt tcttactgta attactaatt tcctcataag ctaatttaca
     2701 gcctaaataa aaatccattg cttttttaga atctggtaat aattgctttg ttgtatcgtt
     2761 tacatataca ccactcaatg gaatctgctt taaaataaac ggtaatacaa ctgaaattct
     2821 atatttatct tttaatattt caggagttat ttgagatgtt atcggcttta cttcttcttt
     2881 gatttcactt tttacgctat ccttaatttc ttctataaca tcaaaaggaa ttacttcttt
     2941 ttttatagtg tcttttacaa tattaggttc tactatctca gtaacgtttg caccatttga
     3001 actaccgctt ttttttccag cagcacaaga tgtaaacatc ccaataacca ataaaaataa
     3061 tactatttta ttattcccac tcaatagttg caggtggttt tgaactgata tcatagacaa
     3121 cacgatttat tcctcttacg ttattaataa ttttatttga aacttttgcc aagaactcat
     3181 agggcaaatg acaccaatct gcagtcattc catcaacaga actaacagca cgcaaggcta
     3241 caacttgttc gtaagtacgc tcatcgccca tcacaccaac agattgaatt ggtaacaata
     3301 tcactccagc ttgccaaaca tcattgtata aattatcttc ttttaaacta ctgataaaaa
     3361 tatcatctgc taattgtagc atttcaactt tttctttagt aatatctccc aatattctaa
     3421 ttcctaaacc tggtccagga aatggatgac gatttaagat attttcaggc aattccaaag
     3481 ctttacctac tctccttact tcatctttaa atagttttct taaaggctct acaattttca
     3541 aatgcatttt ttctggtaac cctccgacat tgtggtgtga ttttattttt gcagaaggtc
     3601 catgaacaga aactgactct atcacatctg gatagatagt accttgtgct aaataagtta
     3661 cattttccaa tttattggct tctctttgaa atatatctat aaaaaccttg ccaattattt
     3721 ttcttttagc ttcaggctct gaagttcctg ctaattcaga ataaaaatca ttcgacgcat
     3781 caacacctat gatattcaaa cccaattgct tataagattc taaaactgaa tcatattcat
     3841 tatatctcaa taaaccatta ttaacaaaaa tgcaatatag ttgctgtcca atggctttat
     3901 gcaataatag agcagccaca cttgaatcaa cacctccact taaacccaaa acaaccttat
     3961 cgcttccaat ggtttgtttt agctcttgga cagtttcctc aataaatgac aattcagacc
     4021 aatcttgctg acaaccacaa atatctacta caaaattttt caataaagtt ttaccatcaa
     4081 tggaatgggt aacttctgga tgaaattgaa taccaaatac atttttattt ttccatttaa
     4141 aagctgcaac tggaatatca tcagtagaag ctaataattc aaaatcatca ggcaactgaa
     4201 tgatagaatc gccatgcgac atccacactt gacttttttg tggaatatct ttcaacaaat
     4261 ctgctccttg aagaatattg atattagcac gaccatattc tcttttagaa gatttttcta
     4321 ctgtgccgtt cattgttttt gccaagtatt gagctccata gcaaacacct aaaatcggca
     4381 aattatatgc tttaataaaa tctaaaatag gaaattccaa agcattatct tgcaatactg
     4441 aaaatggact tcctgaaagg ataattcctt taatattttc agacattaaa tcttgaaatt
     4501 tggtattata aggaacaatt tcgcaataaa catttagttc cctaattcta cgtgcaataa
     4561 gttgagtaaa ctgtgaacca aaatcaaaaa ttagaatttt attatgttgc atggacaaat
     4621 ttaagactat tttgccttag cctacaattt ttgtgtatta attatttatt ttattataat
     4681 cttgagctat aaaatataat ctgatttttt atctacgttc taagatttct ttcaaaactg
     4741 cttgagcagc aaattctcga tttcctgctt gttcaataac taaagactta tcac
//
LOCUS       DAOQCW010000142         4752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_8230_length_4752_cov_5.457100, whole genome shotgun sequence.
ACCESSION   DAOQCW010000142 DAOQCW010000000
VERSION     DAOQCW010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8230_length_4752_cov_5.457100"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="PLS16_10380"
     CDS             <1..693
                     /locus_tag="PLS16_10380"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNJ61532.1"
                     /translation="NNKDIGQVASKIRKAPYFNDLKKLFKISNTKDDQMVMYATMLAL
                     KSFQTSKQVNPYTSKFDFYLQGKVKLSDAEMRGWKLFQDTTKSKCSLCHLSEANKFTN
                     TIVFADFSYDNIGVPKHPSQIHLPLDSGFAKAEFVGKPIVINPLEIGRFKTASLRNIN
                     KTAPYMHNGVFETLEEVLEFYNTRDVNAKYVPEYTPTMNTEDMGDLKLSDTDIADIIA
                     FLKTLDDGYRLK"
     gene            complement(678..1337)
                     /locus_tag="PLS16_10385"
     CDS             complement(678..1337)
                     /locus_tag="PLS16_10385"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNJ61533.1"
                     /translation="MKVCTDACLFGAWINIPKHTKHIVDIGTGTGLLSLMLAQQSEEQ
                     VKIDAVELEENAFEQAINNFAESIFSERIKGIYVNFFNYKTEKKYDILVCNPPFYKQQ
                     FATKNKANILARHSDNFDFNDFFLLCSQLSNEDASLFLLMPFYRKNEIEKIALQNKWY
                     PKEITLVVNKIGQQPIRVMFHFVKNIEIQVLKNELIIYQENNAYTDEFTEKLIPFYFN
                     L"
     gene            complement(1409..1708)
                     /locus_tag="PLS16_10390"
     CDS             complement(1409..1708)
                     /locus_tag="PLS16_10390"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNJ61534.1"
                     /translation="MEIKALPVSGMPDVITAMDLTKYKNGMYKAISGESYIELIQFTK
                     EGVKIESIQPYGQSNKKGSKHYDDQMEMFVKHKLKPMSMDYNELLANNESVYHPK"
     gene            2301..3506
                     /locus_tag="PLS16_10395"
     CDS             2301..3506
                     /locus_tag="PLS16_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61535.1"
                     /translation="MSTQKNLYPIVTLIILIFSLSCEKETNNINSNLKLAKEFHIKTI
                     SKELASFSKALTPIFNNQQNLNLLKEAIYLKKRDENITINDLNKSLESSFNFEQELYT
                     NLTKINKISFEDFKRLIDEYDLTIYWEFIENWDGVTIPKIGYPINEEEDVNNNYDLLH
                     YSVNFDGIEKIIERTYLKNNPVLIIRPNEDFENNSPSYYSKEPYIWLYDLMNKNDYKN
                     AKIEQVPFDRFRLETINTGGSTLDGSGGPELRFFRNSPTSYTNGKYGFATILSLNLTA
                     SASQTTQDIEVKNYISNILHDDWHIIDFRSLIGTYEEDGGLIHNQVVSGIGIEANLLG
                     VPVVNISFNSKISRKDDLLDKYEMYRSSFVYNVQQKNNWGWGFNYMGLPYRKSTNNTS
                     LKVTYKNNY"
     gene            3508..4179
                     /locus_tag="PLS16_10400"
     CDS             3508..4179
                     /locus_tag="PLS16_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61536.1"
                     /translation="MKYIIIYLIILFLNNNISIAQDSNQYKYNNLGTNFYYNTDNNYF
                     FFKPGFILDVYYERVLNRWFGLQTSFGYNRAKTKLNDWKYRFFTEGIADYPSLSSSIL
                     LSMSGNFYCLHKINHKIKIGLGIEYRNITDVITTGAVYLYPDSIQNNYQKLYPITSFE
                     QYNDLGFSGNLNYQYKFKKGIGISSTFGFNYYFLNTSKQARTHNRISDSQLLLKFGIG
                     AFYSF"
     gene            complement(4262..>4752)
                     /locus_tag="PLS16_10405"
     CDS             complement(4262..>4752)
                     /locus_tag="PLS16_10405"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNJ61537.1"
                     /translation="KIEIFTYPKKTIILKKGNICSYLCYIEKGLAHLYSDEGKKMFTN
                     DIVKNGEILTSFASFVSEQPSSENIELLEDSVLQGISFDNLQLLYKKYPELERIGRLV
                     AEHHYNSLAAQTHRLRFYTSAERYNYFYKSKFELVQRVPLGIIASYLGMTLENLSRIR
                     SK"
BASE COUNT         1758 a          659 c          648 g         1687 t
ORIGIN      
        1 aacaataaag atattggaca agttgcttct aaaatcagaa aagctccgta tttcaatgat
       61 ttaaagaaac tttttaaaat tagcaataca aaagatgacc aaatggttat gtatgcgacg
      121 atgttggcct taaaatcttt tcaaacctca aaacaagtca atccatatac ttcaaaattt
      181 gatttttatt tgcaaggaaa agtaaaattg agtgatgccg aaatgcgtgg ttggaaatta
      241 tttcaagata cgacaaaaag caaatgctca ctgtgtcatc tttcagaagc aaataaattt
      301 acaaacacta tcgtttttgc agatttttct tatgataata ttggcgtacc aaaacatcca
      361 agtcaaatac acttaccatt agattcagga tttgctaaag cggaatttgt tggcaaacct
      421 attgttatca atccattaga aattggacga tttaaaactg cctctttaag aaatatcaat
      481 aaaactgcac catatatgca taatggtgta tttgaaactt tagaagaagt cttagaattt
      541 tacaatacaa gagatgtaaa tgctaaatac gtaccagaat atacgcctac aatgaatacg
      601 gaagatatgg gtgatttgaa attaagtgat acagatattg cagatatcat tgctttctta
      661 aaaacattag acgatggcta tagattaaag taaaaaggga ttaatttttc tgtaaactcg
      721 tctgtataag cattgttttc ttgataaatt ataagttcat tttttaaaac ttgtatttct
      781 atattcttta caaaatgaaa cattactcgg ataggttgtt gtcctatttt atttacaact
      841 agagttattt cttttggata ccatttattt tgtaatgcaa ttttttcaat ttcatttttt
      901 ctataaaaag gcatcaataa aaacaaactg gcatcttcat tagacaactg agaacaaagc
      961 aaaaaaaagt cattaaaatc aaaattatca ctatgtctgg ctaagatatt tgctttgttt
     1021 tttgtggcaa attgttgttt atagaacgga ggattacaaa ctaaaatatc atattttttt
     1081 tcagttttat aattaaaaaa attaacataa attcctttta ttctttcaga gaaaatagat
     1141 tctgcaaagt tgtttatagc ttgttcaaaa gcattttctt ctaattctac agcatcgatt
     1201 tttacttgtt cttccgattg ttgtgctaac attaaactta acaatcctgt tcctgtgcca
     1261 atatctacaa tatgtttcgt atgctttgga atatttatcc aagcaccaaa caaacaagca
     1321 tcagtgcata ctttcatggc acatttatct tgatgtacga cgaattgttt gaatttaaaa
     1381 aatgaatttg ccaatacgac aaaagtaatt atttcgggtg atatacactt tcattattcg
     1441 ctaataattc attataatcc atagacatag gtttgagttt atgctttaca aacatttcca
     1501 tttggtcgtc ataatgttta ctgccttttt tattgctttg cccataaggt tgaatacttt
     1561 ctatttttac gccttctttt gtaaactgta tcaattcaat ataagactca ccagaaatag
     1621 ctttatacat tccattttta tattttgtta aatccatcgc cgtaatcaca tcaggcatac
     1681 ctgaaacagg caatgctttg atctccatct actacttttt ttgcctcatc tataaacttt
     1741 cggatagagc atcctgttct tagttcgatg tgcttagcta caactagtgc cataaaacat
     1801 accaaaatgt gaagtttgat aggttcttct ttaaaatgga agatagggta gctttgcacc
     1861 tcgctgtagt agctataaat tatagagaat tggaagatta aaaataatct tgtgcagata
     1921 aattagtgtc atatatattt atctcttcca atataaagta tcttcatctc aataaaatct
     1981 aaaatgctat ctcttatatg aataggcaaa acatatttct taaaataata aatctatttt
     2041 agaaaaagtt ctttttatta tttagattaa cttaagataa caaaacatta aatatgtaag
     2101 gcttttattt taataattta tttttattat atacatttgc aatataacaa ggattatatg
     2161 ttatcacata catacataca tacatacata catacatata acatttattg taagtttatt
     2221 tttatttata cctccttaat atttttaatt accaaaagag aataaacata aaatgataaa
     2281 ataaaaataa attataacaa atgagtactc aaaaaaactt atacccgata gttactttaa
     2341 taatattaat attttcttta agttgtgaga aagaaactaa caatattaac tcaaatttga
     2401 aattagctaa agaatttcat attaaaacta taagcaaaga attagcatca ttttcaaaag
     2461 cattaacacc tatatttaat aatcaacaaa atttaaatct attaaaagaa gcaatttacc
     2521 tcaagaaaag ggatgaaaac attacaatta atgatttaaa taaatcatta gaatcttcct
     2581 ttaactttga gcaagaatta tatactaatt taacaaaaat aaacaagata agttttgaag
     2641 acttcaaaag gttaatagat gaatatgatt tgacaattta ttgggaattt attgaaaatt
     2701 gggatggtgt tacaattcct aaaataggtt atccaataaa tgaggaagaa gatgttaata
     2761 ataattacga tttattacat tatagtgtaa attttgatgg aatagagaaa attattgaga
     2821 gaacctattt gaaaaataat ccagttctta ttatacgacc aaatgaggat tttgaaaata
     2881 actctccatc gtactattct aaagaaccct atatttggct ttatgattta atgaataaga
     2941 acgattacaa gaatgcaaaa atagaacaag taccttttga tagatttaga ttagaaacca
     3001 ttaataccgg aggcagtacg ttagacggaa gtggtggtcc agaacttcga ttttttagaa
     3061 atagtccaac tagttatact aatggtaaat atggatttgc aactatatta agtctaaatc
     3121 taacagccag tgcttcacaa acaactcaag atattgaagt aaaaaattat attagtaata
     3181 tcctgcacga tgactggcat attatagact ttcgaagtct tattggaact tatgaagaag
     3241 atgggggttt aattcacaat caagtagttt ctggtattgg aatagaagct aatttactag
     3301 gtgttcctgt cgtaaatatt agctttaact ctaaaatttc tcgtaaagat gatttattag
     3361 acaaatatga aatgtatcga agttcttttg tttacaatgt tcaacaaaaa aacaattggg
     3421 gatggggatt taattatatg ggactacctt atagaaaatc aactaataat acaagcttaa
     3481 aagtaactta taaaaacaat tactagtatg aaatacataa ttatttattt aataatttta
     3541 ttcctaaata ataacatttc tattgctcaa gatagtaacc aatataaata taacaatttg
     3601 ggtactaatt tttattacaa cacagataat aactattttt tcttcaagcc tggatttatc
     3661 ttagatgttt attatgaacg tgtacttaat cgttggtttg gattgcaaac atcatttgga
     3721 tacaatagag ccaagacgaa actaaatgac tggaagtatc gtttttttac agaggggatt
     3781 gcagattatc caagtctgag ttcaagtatt ctattaagta tgagtggaaa tttttattgt
     3841 ttgcataaaa taaatcacaa aataaaaatt ggattgggta tcgaatatag aaacattacc
     3901 gatgttatta caaccggagc tgtttatttg tatcctgatt caattcaaaa taattatcaa
     3961 aagttatatc caattacatc atttgaacaa tataatgatt tgggattctc agggaactta
     4021 aattatcaat ataaatttaa aaaaggaatt ggaataagtt caacttttgg atttaattat
     4081 tatttcttaa ataccagtaa acaagcaaga acacacaatc ggattagtga cagtcaatta
     4141 ttgttaaagt ttggtatagg tgctttttat tctttttaaa gcaaaattgg aatttgattg
     4201 ggtacaattt attattgtac ccaatcaaaa ttcatccttc ataaataata ataagaaact
     4261 tttatttact cctaattctg ctaagatttt ctaaagtcat tccaagataa gatgcgataa
     4321 taccgagagg cactctttga actaactcaa atttggattt gtaaaaataa ttatatcgtt
     4381 cagcacttgt ataaaaacga agcctgtgcg tttgagcggc taatgaatta taatggtgtt
     4441 cagccactaa acgccctatt ctttctagtt ccggatattt tttgtacaat aattgtaaat
     4501 tatcaaagct aataccttgc aatacactat cttctaataa ttcaatattt tcactggagg
     4561 gttgttctga aacgaaacta gcgaaagatg ttaaaatttc accattttta actatgtcgt
     4621 tcgtaaacat tttcttacct tcatctgaat aaagatgtgc taaacctttt tctatataac
     4681 atagataact acaaatattc ccttttttta aaattatagt cttttttgga taagtaaata
     4741 tttctatttt ct
//
LOCUS       DAOQCW010000143         4582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_8737_length_4582_cov_5.523084, whole genome shotgun sequence.
ACCESSION   DAOQCW010000143 DAOQCW010000000
VERSION     DAOQCW010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8737_length_4582_cov_5.523084"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..1488
                     /locus_tag="PLS16_10410"
     CDS             253..1488
                     /locus_tag="PLS16_10410"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNJ61538.1"
                     /translation="MNYLDIIKIAFQAIKTNLVRTIITCSIIGIGIMALVGILTSVDG
                     LKMYMTNSFSAMGANSFKIRNSSIGFNLDGDNVAPKIYPNITYLEANNFKKMFVNYST
                     SIQYIGSGGATLKNKTTKSNPNIMIIGTDENYIENEGYHLEYGRNFSLRELQQGINVA
                     ILGYSTAKKMFGSNFASIQDNPLVKIDDIQYNVIGVLNEKGSSFITTDNITLIPIVKA
                     RQDFASAKPSYIISVKNGTTDSLGAVVDKAIAVMRNVRKLKPSNENNFDIMKSDSISG
                     MFVEKMGMATIAGFIIGLITLFGAAIGLMNIMMVSVTERTKEIGTLKAIGATNKNILI
                     QFLFEAIVICQLGGLLGIILGVSVGNLVSISIGGTFMIPWNWILLGITFCFIVGLISG
                     IYPAIKAAKQDPIEALRYE"
     gene            1501..1827
                     /locus_tag="PLS16_10415"
     CDS             1501..1827
                     /locus_tag="PLS16_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNJ61539.1"
                     /translation="MKTILLLIISNTFMTFAWYGHLKNPTWPLPKVILLSWGIAFFEY
                     CFLIPANKIGYQVFSVFQLKIIQEIISISIFVLFAYFYFGEKLKWNHFVAFLFVFLAA
                     WFAFRK"
     gene            complement(1838..3094)
                     /locus_tag="PLS16_10420"
     CDS             complement(1838..3094)
                     /locus_tag="PLS16_10420"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNJ61540.1"
                     /translation="MVSIDKDSTAYEISRLTYNLLLDFKVPEDIAKHLNTIVLLVILV
                     ILVYLLQFIVRGIIRGVLRRAQKRTGYSFFGYLLNKRFAHFFALIAPFSLVKNSIPIV
                     FVGFPKWISILDKFADAYMVLMIIWMIMALLSSGAELLKENPAYRQKPLESYLQVLRM
                     IMFLFGAIIMFSIFTGKSPVAFFTAMGAMSAVLLLMFKDTIMGFVASIQVTTNDMVQI
                     GDRITMSKFGADGNVTEINLTTVKVQNFDKTITTIPTYALTSESFQNWRGVEKAGVTR
                     IKRPIYIKQSSIRFVEDTELEKFNQIQGISNYIKEKSAEILAHNELIGADRNVRINGR
                     NLTNLGLFRQYVEWYLRNHPKVRQDMTLMVRQLPVSSIGLPLELYLFTNTTKANEFEN
                     IVSDIFDHLFSSVKYFDLEIYEVASN"
     gene            complement(3291..3893)
                     /locus_tag="PLS16_10425"
     CDS             complement(3291..3893)
                     /locus_tag="PLS16_10425"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013352894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNJ61541.1"
                     /translation="MENLQYGYADLEKAIDKETMEIHFSKHYLGYTNNLNKAVEGKSI
                     ANQSIEEILKNLDMSNGALRNNGGGFYNHRMYFATMSPNGGGTPTGKLAEAINQTFGS
                     FDALKKQLEEAGAKRFGSGWAWLFVNKQGKLQVGSTANQDNPLMPGLDISGTPILGID
                     VWEHAYYLKYQNKRGAYLDAFFSVVDWNKVAENYAKAIAQ"
     gene            4149..>4582
                     /gene="selD"
                     /locus_tag="PLS16_10430"
     CDS             4149..>4582
                     /gene="selD"
                     /locus_tag="PLS16_10430"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020909986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HNJ61542.1"
                     /translation="MEELEIKLTHYSHGAGCGCKIAPAVLDKILHSDTIKFTDKNLLV
                     GNNERDDAAVLDLGNDTALISTTDFFMPIVDDPYDFGRIAATNAISDVYAMGGKPVLA
                     IAILGWPIQKLAPEVAQKVLEGARAVCTQAQISLAGGHSIDSP"
BASE COUNT         1514 a          724 c          725 g         1619 t
ORIGIN      
        1 aatgagatgt gaattcaaaa tgaatctcgc cattggcagg tgttatcacc tgccaataca
       61 cacaagtata gagtatagag aagttttgta aaaggtgata acaccttttg agagcaaagt
      121 gtgggattga taatttctgc aaaaggagat aacacccttt gaaagcagga tagggtttcg
      181 ataaaggaaa taatattcat tgattaattg gtacattatt taattcattc attaaatgat
      241 tatctttgtt tagtgaatta cctagatatt attaaaattg cttttcaggc tataaaaaca
      301 aatttagtac gtactataat aacttgcagt attattggta tcggtattat ggctttggta
      361 ggtattctaa cttctgttga tgggcttaaa atgtatatga caaatagttt tagtgcgatg
      421 ggtgccaatt catttaagat tagaaatagt agtattggtt ttaatttaga tggggataat
      481 gttgctccaa aaatataccc gaatattact tatctggaag ccaataattt caagaaaatg
      541 tttgtaaatt atagcacatc tatccaatat attggttcag gcggagcaac cctgaaaaat
      601 aaaacgacaa agtctaatcc taatattatg attattggga cagatgaaaa ttatatcgaa
      661 aatgaaggtt atcatttaga atatggtcga aattttagtt taagagaatt acagcaaggt
      721 attaatgtcg caattcttgg gtattctact gcaaaaaaaa tgtttggctc aaatttcgct
      781 tcaattcaag acaatccttt ggtaaaaatt gatgatattc agtataatgt aattggcgtt
      841 ttaaatgaaa aaggctctag ttttatcacg actgataata tcacattaat tccgattgta
      901 aaagcaaggc aagattttgc aagtgcaaaa ccatcttata ttattagtgt aaaaaatgga
      961 acaacagata gtcttggtgc agtagtcgat aaagcaattg cagtgatgcg gaatgttaga
     1021 aagttaaagc ccagtaatga aaataacttt gatattatga aaagtgatag tatctctggt
     1081 atgttcgtag aaaaaatggg tatggcaacc atagctggat ttattattgg attgataaca
     1141 ttatttggtg cagcaattgg actaatgaat ataatgatgg tcagcgtaac ggaacggaca
     1201 aaagagattg gcacactaaa agctattggt gcaacaaaca aaaatatttt aatacagttt
     1261 ttatttgaag caattgttat ttgtcaatta ggtggacttt taggaattat cttaggtgtt
     1321 tctgttggta atttagtttc tatttctatt ggtgggactt ttatgattcc ttggaattgg
     1381 atattattag gtattacttt ttgttttatc gttggtttga tttcaggaat ttacccagct
     1441 attaaagccg ccaaacaaga cccgattgaa gctttacgat atgaataact aactgatttt
     1501 atgaaaacca ttctattact aattatttct aatactttta tgacttttgc ttggtatgga
     1561 catcttaaaa atcctacttg gccattgccc aaagtaattt tattgagttg gggaattgcc
     1621 ttttttgagt actgtttttt aattcctgct aataaaattg gctatcaagt attctctgta
     1681 tttcaattaa aaattattca agaaattatt tctatttcta tttttgtttt atttgcatat
     1741 ttctattttg gcgaaaaatt gaaatggaat cattttgtag cgtttctatt tgtttttcta
     1801 gcagcttggt ttgctttcag gaaatagtta gtttggctta attactagct acctcgtaaa
     1861 tttctaaatc aaaatacttt actgaggaaa ataaatgatc aaaaatatca ctcacaatat
     1921 tttcaaactc attagctttg gtcgtatttg taaacaaata caattccaaa ggcaatccta
     1981 tactagaaac gggcaactga cgaaccatta aggtcatatc ttgtcttact ttcgggtgat
     2041 tgcgtaagta ccattctaca tactgtctga acaatcctag attcgtcaaa tttctaccat
     2101 taattcgaac atttctatct gctccaatta attcattatg ggccaaaatt tcggcacttt
     2161 tttcttttat gtaattggat attccttgta tttgattaaa tttttctaac tctgtatctt
     2221 ccacaaaacg aatagatgat tgtttgatat atattggtcg ttttattctt gttacacctg
     2281 ctttttctac acctctccaa ttttgaaaag attctgaagt caaagcataa gttggaatag
     2341 ttgtaattgt tttatcaaaa ttttgaactt ttacagttgt taaattgatt tccgtaacat
     2401 ttccatctgc tccaaattta ctcattgtaa tcctatcgcc aatctgaacc atatcatttg
     2461 tggtaacttg aatacttgca acaaaaccca taatggtatc tttaaacatc aataacaata
     2521 cagctgacat tgctcccatt gcagtaaaaa atgcgactgg agatttgcct gtaaagatgg
     2581 aaaacataat gatagctcca aatagaaaca ttatcattcg caatacttga agataacttt
     2641 ctaaaggttt ttgtctatat gcaggatttt cttttaacaa ttcggcacca gatgacaata
     2701 aagccattat catccaaata atcattagta ccatataggc atctgcaaat ttatctaaaa
     2761 tagatatcca tttaggaaat cctacaaata caataggaat tgaattttta actaaactaa
     2821 atggtgcaat taatgcaaaa aaatgagcaa atcgtttatt cagtaaatat ccaaagaatg
     2881 aataaccagt ccttttttgt gctcttctta aaacacctcg aataattcct ctcacaataa
     2941 attgtaaaag atagactaga attaccaaaa taaccagcaa cactatggta ttgagatgct
     3001 ttgctatatc ttcaggtact ttaaaatcaa gcaataaatt ataagttaat cgacttattt
     3061 cataagccgt gctatcttta tctatactta ccattttact tcaaaattat gaggcaaaga
     3121 tattgaatcc aatacaatta aaattcaagg atatattgta aaatgttaaa tatctatgat
     3181 ttacactaac ttattttcaa cacaatactt acttaacttc tattaaccta aacaaaataa
     3241 ataatccacc gaaaggcggt ggatttttta atatattata tattctaagt ctattgagct
     3301 atcgcttttg cataattttc agctacttta ttccagtcca ctacgctgaa aaaagcatct
     3361 aaataagcac ctcgtttatt ttgatatttt aaataatagg cgtgttccca aacatctatt
     3421 cccaaaattg gcgtacctga aatatctaaa ccaggcatca atgggttatc ttgatttgct
     3481 gtagaaccca cttgcaattt tccttgttta ttgacaaaca accaagccca acctgaaccg
     3541 aatctttttg caccagcttc ttccaattgc ttttttaaag catcaaaaga accaaaggtc
     3601 tgattgatag cttctgctaa ttttcctgtt ggcgttccgc ctccatttgg actcatcgtt
     3661 gcaaagtaca ttctgtggtt ataaaaacca ccaccattat tgcgtaatgc accgtttgac
     3721 atatctaaat tttttagaat ttcttctatg gattgattag ctatggattt tccttcaaca
     3781 gctttgttta aattatttgt ataacctaag tagtgctttg aaaaatgaat ttccattgtt
     3841 tctttatcaa tggctttctc taaatccgca taaccatatt gtagattttc aatttggaat
     3901 ggaccactcg ccgcttttac atcagctgga tttcctactt ttacttccgt aggtttcaca
     3961 caagtagtag ctgccttttt ttgacaacaa gatttctttt ttccttcttt tgcagttgca
     4021 acactaaaag ttaagcctat tgcacacatt aatataaata atttcttcat aaacataaat
     4081 ttttccaaaa ttagctaatt gtataaggct tccctaattt ttaacaatta ttatcattag
     4141 ttttgagaat ggaagaacta gaaattaaac tgactcatta ctctcatggg gctggttgtg
     4201 ggtgcaaaat tgctcctgca gttttagata aaatattaca cagcgataca ataaaattta
     4261 ctgataagaa tttattggta ggaaataatg aaagagatga tgccgctgtt ttagacttag
     4321 gaaatgatac cgctcttatt tctactactg atttttttat gccaattgtt gatgaccctt
     4381 atgattttgg aagaattgct gccacaaatg caattagcga tgtgtatgcc atgggtggaa
     4441 agccagtttt agcaattgct attttaggtt ggccaataca aaaactagct ccagaagtgg
     4501 cacaaaaagt attggaaggt gcaagagctg tttgcacaca agctcaaatt agtttagctg
     4561 gtggacatag tattgatagc cc
//
LOCUS       DAOQCW010000144         4387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_9425_length_4387_cov_5.065559, whole genome shotgun sequence.
ACCESSION   DAOQCW010000144 DAOQCW010000000
VERSION     DAOQCW010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9425_length_4387_cov_5.065559"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PLS16_10435"
     CDS             <1..606
                     /locus_tag="PLS16_10435"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HNJ61543.1"
                     /translation="IASSEETKTNDSIYSKILPLDSLITSNKNFKSLDKKKTSKNNTI
                     PKPRFFKDSTRTSAKDAALLGGMFPGLGQIYNRKYWKLPIVYGGIAGSIYWLASSAIK
                     TKEYNGYLVKAAKNEPLPPNIAIYGASQIESIRNTHRRNVQLASFVTVMVWGFTIIDA
                     AVDAHIRPFDVSDNLSMQIKPSIHQFQQENYFALSVNLNLK"
     gene            654..1397
                     /gene="dapB"
                     /locus_tag="PLS16_10440"
     CDS             654..1397
                     /gene="dapB"
                     /locus_tag="PLS16_10440"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNJ61544.1"
                     /translation="MNIALIGYGKMGKAIHEIANQKNEQLGENKYNIQLIVDIDNRAT
                     ITLEDLQKCDVAIEFTSPHTAIDNIKWCFEAGIPVVVGSTGWLDKLDEIKALASSKNQ
                     TILYSPNFSIGVNIFFEMNRQLAKIMNKYDAYDVEMEEIHHTEKKDAPSGTALYAALD
                     VLKFVDRKADWKNEQTKSENTLDIISLREPNVPGTHTVTYNSEIDSIELKHTAHNRKG
                     FAGGAVVAAEWLVSKKGVYTMDDVLGFNQ"
     gene            complement(1448..2734)
                     /locus_tag="PLS16_10445"
     CDS             complement(1448..2734)
                     /locus_tag="PLS16_10445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNJ61545.1"
                     /translation="MLVSENTIDVIVIGGGFSGLSTAYYLQKKGLNVKVFDASGRIGG
                     RAKTDQIDGFLLDWGTHFYHHSTTELQKIINLTKLGLENIYPGYLLRHNDKFLLYSNP
                     MLKTIDVFPAIFANNISIKDKLRLFGFSLKLKALSYSNIVKQTEMSTLQYFKRAGFSK
                     QTIDTLFKPIISSAIFDRSLQSSSKLSLLFLKSLFNDFVSLPKNGIGSIAEAMVKELA
                     PASVCLKSRVKSISEEGVEFSDGRLIKSKAVVIATNAIDAKKICCKIDLNTESTHVST
                     IYFETEHPPVSKPVMVLNANEHGLVNHVFVPSLLHANYAPKGKHLVAVNIIKSNELDD
                     DELIAEVMVELSGWFGLQVKDWRHIKTYHIKYAMPFKPIIDDVEFTKHLKDNIFYAGD
                     ALSIGTMESALRSGRETAENVIQYLKNEKKLSSLNK"
     gene            complement(2839..>4387)
                     /locus_tag="PLS16_10450"
     CDS             complement(2839..>4387)
                     /locus_tag="PLS16_10450"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HNJ61546.1"
                     /translation="ISIDRRSNLNFVNDYMLKQSFTEVKNIGWFMEHEERATNIAFSK
                     RKNARSFRLYRSISRENIQLNEPIPDTIFTEPKYYKEDGYRKKTDSFWVDNRHDTLSP
                     AESKIYVLMDSLMNTKFMKRIMKFGEFLSSGYYRINKFQVGGLHQFFSLNDVEGYRFR
                     MGFKSNWRLSEKIQFKTYLAYGTLDKKVKYGGNIDFRLPNKKQIYNIFGFSYKDDYQR
                     FSLEPNALEYDHIINVLTRKKNIPDLIYVRQVQAYYKRQIFSDYNIGVNLTYKKFFTV
                     PGKIEFNKTMPDSSVVSHQSFKIFSPQFIFSGTPGAKMLSTANRQVFLKGKLPRFTFT
                     YTPSFKNLLSDFSFHRLELVIEERLPTPIGRTFILFEASKLFGNAPYPLLVIHPGNQS
                     WLSQYRRFNMMNESEFAADQQITFILEHHFDGFFFNKIPLWKKLGLREVFFTKMAVSS
                     LDKNKIGFSDLPSNMKGLNGFYAEIGFGIESILKLFRVDFNWRLTQLDHANAKKFRVT
                     FAFSPNL"
BASE COUNT         1545 a          675 c          671 g         1496 t
ORIGIN      
        1 attgcatctt cagaagaaac taaaaccaat gactcaatat attctaaaat attgccattg
       61 gatagtttaa taactagcaa caaaaatttt aaatctttag ataaaaagaa aacatcaaaa
      121 aataatacaa ttcctaaacc aagatttttt aaagattcta ccagaacatc agccaaagat
      181 gctgctttgc tcggtggaat gtttccaggt ttaggacaaa tttataatag aaaatattgg
      241 aaattaccca tagtttatgg tggaattgcg ggttcaattt attggttagc atcatcagcc
      301 atcaaaacaa aagaatacaa tggttattta gtaaaagcag caaagaatga accattgcca
      361 ccaaatattg ctatttatgg tgcaagtcaa attgaaagta taagaaatac acatcgtaga
      421 aatgtacaat tagcatcgtt tgtaaccgta atggtttggg gatttacaat aatagatgct
      481 gcagttgatg ctcatatccg accatttgat gtatccgata atttatctat gcaaattaaa
      541 ccgagcatac atcagttcca acaagaaaat tattttgctt tatctgtgaa cttaaaccta
      601 aaataaccaa gaataccgtt ttctttttct atttttgaac taaaatttaa gacatgaata
      661 ttgcacttat tggatacggg aaaatgggta aagcaattca cgaaattgcc aatcaaaaga
      721 atgaacaatt aggcgaaaat aaatacaata ttcaattgat tgttgatatt gataatagag
      781 caacaataac tttagaagat ttacaaaaat gcgacgtagc aatagagttt acatctccgc
      841 atacggcaat tgataatata aaatggtgct ttgaagctgg aattcctgtt gtagttggca
      901 gtacaggttg gttggacaaa ttagatgaaa ttaaagcatt agcaagctct aaaaatcaaa
      961 cgattttata ctctccaaat tttagcattg gagtgaatat cttttttgaa atgaacagac
     1021 aattggcaaa aattatgaat aaatatgatg cttatgatgt cgaaatggaa gaaattcacc
     1081 atacagaaaa aaaagatgca ccaagtggaa cagctctcta tgctgcatta gatgttttaa
     1141 aattcgtaga tagaaaagct gattggaaaa atgagcaaac aaaatcagaa aatactttag
     1201 atattatttc tttgagagag ccaaatgttc caggaacaca tacagtaact tataattctg
     1261 aaatagattc tattgagcta aaacatacag cacacaatag aaaaggcttt gcaggcggag
     1321 cagtagttgc tgccgagtgg ttggttagca aaaaaggtgt ttatacgatg gacgatgttt
     1381 taggctttaa tcagtaaaaa aatatataat ttcactgtag tttttttatt caaaagtaaa
     1441 agcaaattta tttatttaag gaactgagtt tcttttcatt ctttaaatat tgaatcacat
     1501 tttctgctgt ttctcgtccg cttcttaatg cagattccat tgttccgatt gataaagcat
     1561 cgccggcata aaaaatatta tcttttagat gtttagtaaa ttcaacatca tcaataatag
     1621 gtttgaaagg cattgcatat ttgatatgat aagtttttat gtgtcgccaa tctttaactt
     1681 gcaaaccaaa ccaaccagaa agctcaacca taacttcagc aattaattca tcatcatcta
     1741 attcattaga tttaataata tttacagcga ctaaatgttt gccttttggt gcatagttgg
     1801 catgcaataa acttggtaca aatacatgat tgactaatcc atgttcattt gcatttaaaa
     1861 ccataacagg tttagaaaca ggtggatgtt ctgtctcaaa gtatattgta ctcacgtgtg
     1921 tactttctgt atttaagtct attttacaac aaattttctt tgcatcaatt gcatttgttg
     1981 ctattacaac cgcctttgat tttattaatc tgccatcaga aaattcaaca ccttcttctg
     2041 aaatgctttt taccctagat tttaaacaaa ctgatgctgg tgctaattct tttaccattg
     2101 cttcagcaat acttccaata ccatttttag gaagagaaac aaagtcatta aacaatgact
     2161 ttaagaataa taaagagagt ttagaagaac tttgtaaact tctatcaaaa atagcagaag
     2221 agataattgg cttaaaaagt gtatcaatag tttgtttaga aaaaccagcc cttttgaaat
     2281 attgtaaagt ggacatttct gtttgtttca caatattaga ataggagagt gcttttaact
     2341 ttaagctaaa tccaaataag cgaagtttgt cttttataga gatattattg gcaaatatcg
     2401 caggaaaaac atctatggtt tttaacatcg gatttgaata gagtaaaaat ttatcgttat
     2461 gtcttaataa gtaacctgga taaatatttt ctaatcctaa ttttgttaaa ttaattattt
     2521 tttgaagctc tgttgtagaa tggtgataaa aatgtgtacc ccaatctaat agaaatccat
     2581 ctatttggtc tgtttttgct ctgccaccta tgcgtcctga tgcatcaaaa acttttacat
     2641 taagtccttt tttttgcaaa taatatgcag ttgaaagtcc agagaaacca cctccaatta
     2701 caattacatc gattgtattt tcgcttacca acattaatac aaagatacaa agggttcatt
     2761 ctgttattat aaagtgaaaa ttatcaatac attaaaacag tattttgctc ataaataaag
     2821 taataaaaat cttaatcttt ataggttagg actaaacgca aaagtaaccc taaatttctt
     2881 tgcatttgca tggtctaatt gtgtcaatcg ccaattgaaa tctactctga ataattttag
     2941 aatactttct attccaaatc caatttctgc ataaaaacca ttcaatcctt tcatatttga
     3001 cggtaaatct gaaaaaccaa ttttattttt atctaatgaa ctaactgcca ttttagtaaa
     3061 aaacacctca cgtaacccta attttttcca aagtggaatt ttattaaaaa agaaaccatc
     3121 aaaatgatgt tctaaaataa atgtaatttg ttggtcggca gcaaattcag attcattcat
     3181 catattaaat cgtctgtatt gtgagagcca agattgatta ccaggatgta taaccaatag
     3241 tggatatggt gcattgccaa ataatttact tgcctcaaat agtataaaag ttcttccaat
     3301 aggcgttgga agtctttctt caatgactaa ttctaagcga tgaaaactga aatcacttaa
     3361 aaggttttta aaacttggcg tataagtaaa agtaaatcgt ggtagttttc cttttaagaa
     3421 aacttgtcta tttgcagtag atagcatttt tgctcctggt gttccagaaa aaataaattg
     3481 aggtgaaaat attttaaaac tttgatgtga tacgacacta ctatccggca ttgttttgtt
     3541 gaattcaatt ttaccaggaa ctgtaaagaa ttttttataa gtcaaattaa caccaatatt
     3601 gtaatcagag aaaatttgcc ttttgtaata tgcttgaact tgtcttacat aaattaagtc
     3661 aggaatattt ttcttgcgtg ttaaaacatt tatgatatgg tcatattcta aagcgtttgg
     3721 ttctaaggaa aatctttgat aatcgtcttt gtaggaaaat ccaaatatat tataaatctg
     3781 ctttttatta gggagtctaa agtcaatatt tccaccgtat ttgactttct tgtctaatgt
     3841 accataggct agataggttt tgaattgtat tttttcactt agtcgccaat tggatttaaa
     3901 acccattcta aatcgatagc cttcaacatc attcaagcta aaaaattgat gtaaaccacc
     3961 aacttggaat ttattgatac gataataacc agaggaaaga aattctccaa atttcattat
     4021 acgtttcata aacttcgtgt tcatcaaaga atccatcaat acataaattt tggattctgc
     4081 tggagaaagc gtatcatgtc ggttatcaac ccaaaagcta tctgtctttt tacgatatcc
     4141 atcttcctta tagtatttgg gttctgtaaa tatggtatct ggaataggtt cgtttagttg
     4201 tatattctcc ctagatatcg aacggtagag tctgaaactt ctagcattct tccttttgga
     4261 aaatgcaata ttagtggctc tttcttcgtg ttccataaac catccaatat tttttacttc
     4321 tgtaaaactt tgtttcagca tgtagtcatt cacaaaattt aaattggaac gtctgtctat
     4381 gctgatt
//
LOCUS       DAOQCW010000145         4314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_9708_length_4314_cov_5.743367, whole genome shotgun sequence.
ACCESSION   DAOQCW010000145 DAOQCW010000000
VERSION     DAOQCW010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9708_length_4314_cov_5.743367"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..279
                     /gene="infA"
                     /locus_tag="PLS16_10455"
     CDS             61..279
                     /gene="infA"
                     /locus_tag="PLS16_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNJ61547.1"
                     /translation="MAKTDLIKLDGTISEALSNAMFRVKLENEHEILATISGKMRMHY
                     IRILPGDKVAVEMSPYDLTRGRITFRYK"
     gene            288..404
                     /gene="ykgO"
                     /locus_tag="PLS16_10460"
     CDS             288..404
                     /gene="ykgO"
                     /locus_tag="PLS16_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HNJ61548.1"
                     /translation="MKVRASVKKRSEDCIIVRRKGKIFVINKKNPKFKQRQG"
     gene            410..787
                     /gene="rpsM"
                     /locus_tag="PLS16_10465"
     CDS             410..787
                     /gene="rpsM"
                     /locus_tag="PLS16_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNJ61549.1"
                     /translation="MARIAGIDLPKNKRGFIGLTYIYGIGSSTADYILEKANVDRMKK
                     VHEWNDDEQTAIRNVISNEIKVEGALRSEIQLNIKRLMDIGSYRGIRHRKGLPVNGQS
                     TKRNSRTRKGKRKTVAGKKKATK"
     gene            812..1201
                     /gene="rpsK"
                     /locus_tag="PLS16_10470"
     CDS             812..1201
                     /gene="rpsK"
                     /locus_tag="PLS16_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNJ61550.1"
                     /translation="MAQAKKQVKKRVVKVEAEGFAYVNATFNNIIISLTNKQGQVISW
                     SSAGKGGFRGSKKSTPYAAQVAAHDAGQKAYDAGLRKVEVFVKGPGGGRESAIRTIAA
                     TGIEVTVIRDVTPLPHNGCRPPKKRRV"
     gene            1256..1864
                     /gene="rpsD"
                     /locus_tag="PLS16_10475"
     CDS             1256..1864
                     /gene="rpsD"
                     /locus_tag="PLS16_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012010904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNJ61551.1"
                     /translation="MARYTGPRTKKARTFGDAIYGYDKSYEKRKFPPGQHGNGRRRSS
                     QSEYSIQLKEKQKAKYTYGVLERQFLKTFNEADRKHGITGENLMRLLEQRLDNTVYRL
                     GFAPTRSAARQLVSHKHITVNGRILNIPSYTVKVGDVISVREKSKDLELVQDTLATGR
                     VKKYTWLELNSETLEGKYVELPNRDQIPENIKELLIVELYSK"
     gene            1906..2901
                     /locus_tag="PLS16_10480"
     CDS             1906..2901
                     /locus_tag="PLS16_10480"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNJ61552.1"
                     /translation="MAILSFQKPDKILLQKATDFEGTFEFAPLEPGFGNTVGNALRRV
                     LLSSLEGYAITSVKISGVQHEFSTIKGVIEDVTEIILNLKQVRLKRVLSEADAGFEKV
                     YISLKGKDQFVAGDIVAHTNVYEVTNPELVICNMDKSVSLELELTIGKGRGYVPAEEH
                     FVDSSVVGIIPVDSIYTPIKNVKYKIENTRVEQRTDFEKLIIDIKTDGTIHPEDAIKE
                     AAKILIQHLILVSDENITFETESKQQEEVVDEHILHMRKILKTNLEDMDLSVRAFNCL
                     KAAKINSLSELVQYDTNELLKFRNFGRKSLVEIEELIIEKGLHFGMDLSKFKLED"
     gene            2935..3438
                     /gene="rplQ"
                     /locus_tag="PLS16_10485"
     CDS             2935..3438
                     /gene="rplQ"
                     /locus_tag="PLS16_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNJ61553.1"
                     /translation="MRHGNKINHLGRTYGHRSALLKNLSNALIMHKRIETTLAKAKEL
                     RVYVEPLISKSKDNTTHSRRTAFSKLQNKYAAKELFDIIGPKVGERPGGYTRIIKLGK
                     RLGDNAEMALIELVDFNEIYSANKSAQTTTDTKKRTRRATSKKKQTEETQDAVESTET
                     PTENAEA"
     gene            3550..>4314
                     /locus_tag="PLS16_10490"
     CDS             3550..>4314
                     /locus_tag="PLS16_10490"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HNJ61554.1"
                     /translation="MQKAILLLEDGTHFEGLACGKIGTTTGELCFTTTMTGYQEIFTD
                     PSYNGQLLILTNVHLGNYGIKDSETESDKIQINGIICREFTNNYSRDQANSNIQNYFE
                     ANNLVGITNIDTRAIVNYIREKGTMNAILSSETDDIAVLKAKLQNVPSMEGLELASVV
                     STKNTYTVGDESSAKYRIAALDFGIKRNILNNFVSRNAFVKVFPANTSYAEMMQFNPD
                     GFFISNGPGDPAVMDYAVKTIQEILEHNHPIFGICLG"
BASE COUNT         1664 a          533 c          785 g         1332 t
ORIGIN      
        1 taaaaaagtc tgattatatt agtgatttct agatatttat tacgatattt gcactacaat
       61 atggctaaaa cagatttaat aaaattagac ggaacgatat cagaagcatt atctaatgca
      121 atgtttagag tgaagttgga aaatgaacat gaaatattgg caacaatatc tggtaaaatg
      181 cgtatgcact acatcagaat tttgccagga gataaagtag cggtagagat gtcgccttat
      241 gatttaacaa gaggaagaat aacatttaga tacaaataaa tagaaaaatg aaagttagag
      301 catcagtaaa gaaaagaagc gaagactgca tcattgtacg cagaaagggc aaaatttttg
      361 taattaataa gaaaaatcca aaatttaaac aaagacaagg ttaataatta tggcaagaat
      421 agcgggtatt gatttaccaa aaaacaaaag aggcttcatc gggttgacat acatttatgg
      481 aattggtagt tcaactgctg attacatttt agaaaaagcc aatgtagatc gaatgaagaa
      541 agttcatgaa tggaatgatg atgaacaaac tgccattcgt aatgtaatct ctaatgaaat
      601 taaagttgaa ggtgctttgc gttctgaaat tcaattgaat atcaaacgtt taatggatat
      661 tggttcttac agaggaatta gacatcgtaa aggtcttcca gtaaatggac aaagtactaa
      721 acgtaactct agaactagaa aaggaaaacg taagactgta gctggaaaga aaaaagcaac
      781 taaataatca taaataaaat aactgaaagt aatggctcaa gctaaaaaac aagttaagaa
      841 aagagtagtt aaagtagaag cagaaggttt tgcttatgta aatgctactt ttaataatat
      901 tattatctct ttaacgaaca aacaaggtca ggtaatttct tggagtagtg ctggtaaagg
      961 tggatttaga ggttctaaaa aaagtacacc ttacgctgct caagttgctg cacatgatgc
     1021 aggtcaaaaa gcttatgatg caggattgag aaaagtagaa gtatttgtta aaggaccagg
     1081 tggtggtaga gaatctgcta tcagaacgat tgctgcaaca ggtattgaag taacggtaat
     1141 tagagatgtt acacctttac ctcacaatgg ttgtcgtcca cctaaaaaac gtagagtata
     1201 atttgattta aaatgtagat gtttttttag acattagtaa aatatatagt aaaaaatggc
     1261 aagatataca ggacctagaa ccaaaaaagc gagaactttc ggagacgcaa tttatggtta
     1321 tgacaaatct tatgagaaac gtaaatttcc tccaggacaa cacggaaatg gtagaagacg
     1381 ttcatctcaa tctgaatatt caattcagtt gaaagaaaaa caaaaagcta aatacactta
     1441 tggtgtttta gaaagacaat tcttgaaaac atttaatgaa gcagatagaa aacacggtat
     1501 cacaggtgaa aacttaatgc gtttattaga acaaagatta gataacactg tttatagatt
     1561 aggatttgct ccaactagaa gtgctgctag acaattagtt tctcacaaac atattacagt
     1621 taatggtaga attttaaata ttccatctta tactgtaaaa gtaggtgatg taattagtgt
     1681 tagagaaaaa tctaaagatt tagagttagt tcaagatact ttagcaactg gtagagtaaa
     1741 aaaatatact tggttagaat tgaattctga aactttagaa ggtaaatatg ttgagttgcc
     1801 taatagagat caaattcctg aaaatatcaa ggagttatta atcgtcgagt tatattctaa
     1861 ataattttta caaataatat ttgtaagtaa aataaataaa gtaaaatggc tatattatca
     1921 tttcaaaaac cagataaaat cttattacag aaagcaactg attttgaagg tacatttgaa
     1981 tttgcacctt tagaaccagg ttttggaaac actgtaggta atgctttaag aagagtttta
     2041 ctttcttctt tagaaggtta tgcaattaca tctgtgaaaa tttcgggtgt tcaacacgag
     2101 ttttctacta ttaaaggtgt tattgaagat gttactgaaa ttattcttaa cttaaaacaa
     2161 gtaagattaa aaagagtgct ttcagaagct gatgcaggat ttgaaaaagt atatatttct
     2221 ttaaaaggaa aagaccaatt tgttgctggt gatattgttg ctcataccaa tgtttatgag
     2281 gtaacaaatc cagagttagt tatctgtaat atggataaaa gcgtaagctt agaattggaa
     2341 ttgaccattg gaaagggtag aggatatgtt ccagctgaag aacattttgt tgattcttct
     2401 gttgtgggta ttattcctgt tgattcaatc tatacaccaa ttaaaaacgt taagtataaa
     2461 atcgaaaata ctcgtgtaga gcaaagaact gactttgaga aattaattat cgatattaaa
     2521 actgatggta ctattcatcc tgaagatgca ataaaagaag ctgctaagat tttaattcaa
     2581 catttaatct tagtttctga tgagaatatt acatttgaaa cagaaagtaa acaacaagaa
     2641 gaagttgttg atgagcatat cttacacatg agaaaaatct taaaaacaaa tttagaagat
     2701 atggatttat cagttagagc attcaattgc ttgaaagctg ctaaaattaa ttcattatct
     2761 gaattagttc aatatgatac taacgaatta ttgaaattta gaaatttcgg tcgtaaatca
     2821 ttagtagaaa ttgaagaatt aatcattgag aaaggtttgc attttggtat ggatttatct
     2881 aaattcaaac tagaagacta aaataaatat tagaaattaa ctcggtataa tataatgaga
     2941 cacggaaata aaattaatca tttaggtaga acgtacggac acagaagtgc tttattaaag
     3001 aatttgtcaa atgctttgat tatgcacaaa agaattgaga ctactttagc aaaagcaaaa
     3061 gaactgagag tttatgttga gcctttgatt tcaaaatcaa aagataatac aacacattct
     3121 agaagaactg cttttagtaa attacaaaat aaatatgcag ctaaagaatt atttgatata
     3181 attggaccaa aagttggtga aagacctggt ggttatacaa gaattattaa attaggtaaa
     3241 cgtttaggtg ataatgcaga aatggctttg attgaattag ttgattttaa cgaaatttat
     3301 tctgctaata aatctgctca aactactaca gatactaaaa aacgtactag aagagcaact
     3361 tctaaaaaga aacaaactga ggaaactcaa gatgctgttg aatcaactga aactccaaca
     3421 gaaaacgcag aagcttaaga tgaaaattaa gttatataat gaaaggttcg tattttttac
     3481 gaaccttttt tttatctttg gttttgaatt ataatcagtt ctcttttcaa ttaaataaat
     3541 tcatttttaa tgcaaaaagc aatattatta ttagaagatg gcacacactt cgagggtttg
     3601 gcatgtggta aaattggtac aacaactggt gaattatgtt ttacaacaac catgactgga
     3661 taccaagaga tttttaccga cccaagttat aatggtcagt tattaatctt gacaaatgtg
     3721 catttgggta attatggaat taaggatagt gaaacagaat ctgataagat tcagattaat
     3781 ggtatcattt gccgggagtt tacaaataac tattctcgag atcaagctaa ttcaaatatt
     3841 caaaattatt ttgaagctaa taatttagtg ggtattacca atatagatac tcgtgctatt
     3901 gtgaattata tcagagaaaa aggaactatg aatgcaattt tatcctctga aactgatgat
     3961 attgcagttc taaaagctaa gttgcaaaat gttccatcta tggaaggatt agaattagct
     4021 tcagttgtga gtactaaaaa tacctacaca gtaggtgatg agtcatctgc aaagtataga
     4081 attgctgctt tggattttgg aattaaacga aatatcctta ataattttgt aagtagaaat
     4141 gcctttgtaa aagtgtttcc agcaaatact tcttatgctg aaatgatgca gtttaatcca
     4201 gatggctttt tcatttctaa tggtcctggc gacccagctg ttatggatta tgctgtaaaa
     4261 accattcaag aaattttgga acataatcac ccaatttttg gaatatgctt aggg
//
LOCUS       DAOQCW010000146         4255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_9979_length_4255_cov_4.701667, whole genome shotgun sequence.
ACCESSION   DAOQCW010000146 DAOQCW010000000
VERSION     DAOQCW010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9979_length_4255_cov_4.701667"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..167
                     /gene="rpsS"
                     /locus_tag="PLS16_10495"
     CDS             <1..167
                     /gene="rpsS"
                     /locus_tag="PLS16_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNJ61555.1"
                     /translation="SRASMITPDFVGHTFAVHNGNKFIPVYVTENMVGHKLGEFSPTR
                     QFKGHSGNRK"
     gene            177..605
                     /gene="rplV"
                     /locus_tag="PLS16_10500"
     CDS             177..605
                     /gene="rplV"
                     /locus_tag="PLS16_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNJ61556.1"
                     /translation="MGARKRLSAEARKAANKQICTAQAIAVPTSPRKMRLVADIIRGR
                     NVDEALNILTFSSKHASITLEKLLRSAIANWEQKFNSSAADSQLFVESVSVDGGRVLK
                     RFQPAPQGRAYRIRKRSNHIQLTLASKNQNNVVESNQENN"
     gene            612..1382
                     /gene="rpsC"
                     /locus_tag="PLS16_10505"
     CDS             612..1382
                     /gene="rpsC"
                     /locus_tag="PLS16_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNJ61557.1"
                     /translation="MGQKANPIVNRLGITRGWDSNWYGGKDFSDKLVEDFKIRKYLNA
                     RIQKGGVSKIIIERTLNKITISIHTSRPGIIIGKGGGEVDKIKEEIKKITNKEVQINI
                     VEIKRPEVDAAIVGETICKQLEARVNFRRAVKMAIASAMRLGVEGIKVKCSGRLGGAE
                     MARSEEYKEGRTPLHTFRADIDYAHKEANTIYGIIGVKVWICKGEVLSKRDFSQNIVG
                     DEQKRAPRGVDKRAPRGDRNDRNDRRSGGGERRERKSK"
     gene            1409..1831
                     /gene="rplP"
                     /locus_tag="PLS16_10510"
     CDS             1409..1831
                     /gene="rplP"
                     /locus_tag="PLS16_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNJ61558.1"
                     /translation="MLQPKRVKFRRVQKGRNTGNAGRGFELAFGSFGLKSLDEGRITN
                     RQIEAARIALTRNMKREGKVWIKIFPDKPITKKPAEVRMGKGKGALDHWVALVQPGRL
                     LFEIEGVSKQLAQESLELAAQKLPIKVKFVVRRDYDGQ"
     gene            1844..2056
                     /gene="rpmC"
                     /locus_tag="PLS16_10515"
     CDS             1844..2056
                     /gene="rpmC"
                     /locus_tag="PLS16_10515"
                     /inference="COORDINATES: protein motif:HMM:NF013027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNJ61559.1"
                     /translation="MSKNKLYTEIANTSTEALQSEFTAAKMRLTKMKFNHAVSPLEDT
                     SVLKKAKKEIARMATELNKRKLSNQA"
     gene            2068..2340
                     /gene="rpsQ"
                     /locus_tag="PLS16_10520"
     CDS             2068..2340
                     /gene="rpsQ"
                     /locus_tag="PLS16_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNJ61560.1"
                     /translation="MSAEQTIERKERKTRIGVVESNKMNKTITVKVERKIQHPKYGKY
                     LKKSKSFHAHDENNECNEGDTVKIMETRPLSKTKRWRLVEILERAK"
     gene            2342..2710
                     /gene="rplN"
                     /locus_tag="PLS16_10525"
     CDS             2342..2710
                     /gene="rplN"
                     /locus_tag="PLS16_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNJ61561.1"
                     /translation="MIQSESRLKVADNSGAKEVLCIRVLKGSKSRYASIGDKIVVSVK
                     SAAPNGNVKKKQVSKAVIVRTKKEIKRKDGSYIRFDDNAVVLLNNQDEPRASRIFGPV
                     ARELRDKDYMKIISLAPEVL"
     gene            2713..3051
                     /gene="rplX"
                     /locus_tag="PLS16_10530"
     CDS             2713..3051
                     /gene="rplX"
                     /locus_tag="PLS16_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNJ61562.1"
                     /translation="MKTNTQKRFAPKLKIKKGDTVVVITGASKGKEGKVLEVYPTKER
                     VLVEGVNIVKKHTKPNAQNQQGGIVEQEASIHISNVKLKAGNTVTRVGRKVQEDGKIV
                     RFAKKTGEVI"
     gene            3053..3604
                     /gene="rplE"
                     /locus_tag="PLS16_10535"
     CDS             3053..3604
                     /gene="rplE"
                     /locus_tag="PLS16_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNJ61563.1"
                     /translation="MSYVIRLKKKYKEEVVSKLMEKYNYKSIMQVPKLTKIVVNQGVG
                     IAVGDKKIIDTQVDEVTRITGQKALSTKSKKAISNFKLRENMPIGVMVTLRGDRMYEF
                     LDRLISVALPQVRDFKGVNDKSFDGRGNYTLGIKEHIIFPEIDIDKINRISGMDITFV
                     TTAQTDDEAHALLSEIGIPFKKK"
     gene            3619..3888
                     /gene="rpsN"
                     /locus_tag="PLS16_10540"
     CDS             3619..3888
                     /gene="rpsN"
                     /locus_tag="PLS16_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HNJ61564.1"
                     /translation="MAKESMKARDAKRAKLIAKYKEKREQLKKEGNWEELDKLPKNSS
                     PVRYRKRCKITGRPRGYVGFFGISRIKFRDLANEGKIPGLTKASW"
     gene            3921..>4255
                     /gene="rpsH"
                     /locus_tag="PLS16_10545"
     CDS             3921..>4255
                     /gene="rpsH"
                     /locus_tag="PLS16_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNJ61565.1"
                     /translation="MVIDPISDYLTRIRNAQMAGHTIVDIPASNLKKKLTEILYENGY
                     ILKYKFEDEGSQGLIKIALKYNASTKEPAIRELLRVSRPGLRKYTPADEIPRVKNGLG
                     IAILSTSKGV"
BASE COUNT         1751 a          521 c          826 g         1157 t
ORIGIN      
        1 ggtcaagagc atctatgatt acgcctgatt ttgttggaca tacatttgca gtacataacg
       61 gaaataaatt tatacctgtg tatgtaacag aaaatatggt aggacataag ttgggagagt
      121 tttcgccaac aagacaattt aaaggacatt caggtaacag aaaatagtaa ataacaatgg
      181 gagctagaaa aagattatca gcagaggcta gaaaagcagc aaataaacaa atttgtactg
      241 cacaagcaat tgctgttcca acatcacctc gtaaaatgag acttgttgca gatataatta
      301 gaggaagaaa tgttgatgaa gcattaaata ttttaacatt ttcatctaaa catgcatcta
      361 ttacattaga aaaattattg cgttctgcaa ttgctaattg ggaacaaaaa tttaattctt
      421 ctgcggctga tagtcaatta tttgtagaat cagtatctgt agatggtggt agagtattaa
      481 aaagatttca acctgcacct caaggtagag cttatagaat tagaaaacgt tctaatcata
      541 tccagcttac tttagcaagt aaaaatcaaa ataatgttgt agaatctaat caagaaaata
      601 attaagaatt aatgggacaa aaagcaaatc caatagtaaa tcgtttaggt atcacaagag
      661 gttgggactc taattggtat ggtggaaaag atttcagcga taaattagtt gaagatttca
      721 aaatacgtaa atacttaaat gcccgtattc aaaaaggtgg agtttctaaa attatcatcg
      781 aaagaacttt aaacaaaatt actatttcta ttcatacatc tcgtccaggt atcatcattg
      841 gaaaaggtgg aggtgaagtt gataagatca aagaggaaat taaaaaaatt acaaataagg
      901 aagttcaaat caatatcgtt gagataaaac gacctgaagt agatgctgca atcgttggcg
      961 aaacaatatg taagcaatta gaagctcgtg tgaatttcag aagagcggta aaaatggcta
     1021 ttgcatcagc aatgagatta ggtgttgaag gaattaaagt taaatgttct ggtagattag
     1081 gtggtgctga gatggcaaga agtgaagaat ataaagaggg aagaacacca ttacatacat
     1141 tcagagctga tattgattat gctcataaag aagcaaatac aatttatggt atcatcggtg
     1201 ttaaagtttg gatttgtaaa ggcgaagttt tatcaaaaag agatttttca caaaatattg
     1261 ttggtgatga acaaaaaaga gcaccaagag gcgtagataa gagagcacca agaggcgata
     1321 gaaacgacag aaatgataga agatcaggtg gcggtgaaag aagagaaaga aaaagtaaat
     1381 aataattata aatcattaac ttagtaaaat gttacaacct aagagagtaa aatttagaag
     1441 agttcagaaa ggtagaaata ctggtaatgc tggtagaggc tttgaattgg ctttcggttc
     1501 tttcggctta aagtctttag atgaaggaag aattacaaat agacaaattg aagctgcacg
     1561 tattgctctt actagaaata tgaagagaga aggtaaagtt tggattaaaa tattcccaga
     1621 taaacctata actaaaaaac ctgctgaagt acgtatgggt aaaggtaaag gtgctttaga
     1681 tcattgggtt gctttagttc agcctggaag acttttattt gagattgagg gtgtttctaa
     1741 acaattagct caagaatcat tagaacttgc agcacaaaaa ctaccaatta aagttaaatt
     1801 tgtagttaga agagattatg atggccaata aaatataatt aagatgtcaa agaataaatt
     1861 atatacagaa atagctaata caagtactga agcattacaa agtgaattta ctgctgctaa
     1921 aatgagatta actaaaatga aattcaatca tgcagttagc cctcttgaag atacaagtgt
     1981 attgaaaaaa gctaaaaagg aaattgctag aatggcaact gaattgaata aaagaaaatt
     2041 atctaaccaa gcataaaaaa gcaagatatg tctgctgagc aaacgataga aagaaaagaa
     2101 agaaaaacca gaattggtgt tgttgaatct aataagatga acaaaacgat tactgtaaaa
     2161 gttgaaagaa aaattcaaca tcctaagtat gggaaatact tgaaaaaaag taaatcattc
     2221 catgctcatg atgagaataa tgaatgcaat gaaggagata cagtaaaaat aatggaaact
     2281 cgaccattaa gtaaaaccaa acgttggaga ttagttgaaa ttttagaaag agcgaaataa
     2341 catgatacag tcagaatcta gattaaaagt tgcagataac agcggagcaa aagaagtact
     2401 ttgtatccgt gtccttaaag gatctaaatc aagatacgca agtatcggtg ataagatagt
     2461 tgtatctgtt aaaagtgccg caccaaacgg taacgttaag aagaaacaag tttctaaagc
     2521 agtaattgta cgtactaaga aggaaattaa aagaaaagac ggttcttata tcagatttga
     2581 tgataatgct gttgttcttt taaacaacca agatgagcct cgtgcaagcc gtatttttgg
     2641 cccagttgct agagagctta gagacaagga ttatatgaaa attatatcat tagcacctga
     2701 agtattataa agatgaaaac aaatacacaa aaaaggtttg cacctaaact caaaattaaa
     2761 aaaggagata cagtagttgt tattactggt gcaagtaaag gaaaagaagg caaagtttta
     2821 gaagtatatc ctactaagga aagagtattg gttgaaggcg tgaatattgt taaaaaacac
     2881 actaaaccaa atgcacaaaa tcaacaagga ggaattgttg agcaagaagc ttctattcat
     2941 atttcaaatg taaaattaaa agctggaaat acagttacta gagttggaag aaaagttcaa
     3001 gaagatggta aaattgtacg tttcgctaaa aaaactgggg aggtaattta atatgagcta
     3061 cgtaataaga ttaaaaaaga agtacaaaga agaagttgta tcaaaattaa tggagaagta
     3121 taattacaaa tccattatgc aagttccaaa attgactaaa attgttgtaa accaaggtgt
     3181 tggtattgca gtgggagata agaaaatcat tgatacacaa gttgatgaag taactagaat
     3241 tactggtcaa aaagctttat ctactaaatc taagaaagcg atttctaact tcaaacttag
     3301 agaaaatatg ccaattggtg taatggttac acttagaggt gatagaatgt atgagttttt
     3361 agatagatta atttctgtag cacttccgca agttagagat ttcaaaggtg taaatgataa
     3421 atcttttgat ggtagaggta attatacttt aggtatcaaa gaacatatta ttttcccaga
     3481 gatagatatc gataaaatta acagaattag tggtatggac attacttttg taactacagc
     3541 tcaaacagat gatgaagctc atgcactatt atctgaaatt ggaataccat ttaagaaaaa
     3601 ataaaaatat catactagat ggctaaagaa tcaatgaaag cacgtgatgc gaaaagagca
     3661 aaacttatcg ctaaatacaa agaaaaacgt gaacaattaa aaaaagaagg taactgggaa
     3721 gagttagata aattacctaa aaattcatct ccagtaagat atagaaaaag atgtaaaatt
     3781 acaggaagac caagaggtta tgtaggtttc ttcggaattt ctagaattaa attcagagat
     3841 ttagctaatg aaggcaaaat tcctggatta acaaaagcaa gttggtaaaa aaaattataa
     3901 acgtataaaa tagaataaag atggttattg atcctatatc agattatctg acaagaatta
     3961 gaaatgctca aatggctgga cataccattg tggacattcc tgcttcaaat ttaaaaaaga
     4021 aattaacaga aattctttat gaaaatggtt atatcttaaa atataaattt gaagacgaag
     4081 gatctcaagg attgattaaa attgcattaa aatataatgc atcaacaaaa gaacctgcaa
     4141 ttagagaact tttaagagtg tctagaccag gtttaagaaa atacactcca gcagatgaaa
     4201 ttcctcgtgt taaaaacggt ttaggaattg ctatattatc aacttcaaaa ggagt
//
LOCUS       DAOQCW010000147         4142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_10487_length_4142_cov_6.040372, whole genome shotgun sequence.
ACCESSION   DAOQCW010000147 DAOQCW010000000
VERSION     DAOQCW010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10487_length_4142_cov_6.040372"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..2016
                     /locus_tag="PLS16_10550"
     CDS             73..2016
                     /locus_tag="PLS16_10550"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HNJ61566.1"
                     /translation="MIIIPETKAMTELENPRIRPVGDLLQENFFVPRYQRGYRWGKQE
                     ITELLDDILQYYNATQNRENKVSKFYCLQPVVVKKKEWFNNSKEKTKGWELIDGQQRL
                     TTILLILNYLEDVRKLLNNNMDIYSIDFETRENCKPFFQDKTYKKTVDETNVDFYHIS
                     KAYEYINKWFEKNNHKVEILNTVLKSDYNVSIIWYEAVDNNQDNDDSSIELFTRLNDG
                     KIPLTDAELIKALLLQADLYPTNEDRFVKQRLFEIATEWDDIEAKLQDEKFWLFLNDT
                     NYQPSSKIEFIFKLLADKWNEFEKQSLVKYNLIDGKPKHYEFLVFDKYLSNKRAAFSD
                     KDEDGKDILEPVNEIWKDIKEIFSIFYEWYNNHNLFHYIGFLLAIEKNKEELIRDLID
                     LKLTKTDFEDNIKNRIANSVKINKKDKESGIIKQLNQIAYGEDNQEIIKVLLLFNVDA
                     LVKHKKENAKFPFHLYKKEKITSIEHIHPQNPPSIDADEDRARIWLSQHKTSLSSMKE
                     FAGSNKDEIDNVLQQTDSLLINYDKEVFKSLFTQTLDLYIKISGFKESELHTLYNLSL
                     VDKDTNSQLNNSFFDIKREILKENKLGRYVPICTQRVFSKYYSNSSKEMIFWNNDDRQ
                     AYFKAIESVYLLFINLIGACNGN"
     gene            2006..>4142
                     /locus_tag="PLS16_10555"
     CDS             2006..>4142
                     /locus_tag="PLS16_10555"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HNJ61567.1"
                     /translation="MATNNTTFWNLINSYKISIPIIQRDYAQGREEEFEKREKFLNAI
                     LKHLNNDKKLHLDFVYGRVKDNTFYPIDGQQRLTTLFLLHWYFALKEKVSSEEKLKLS
                     QFVYDTRISSREFCQSLIKEDIELPITPDDDAFITAIKNKPWYRDKWDTDPTIKAMLV
                     MIQDIHNKFYNINSPSVFTQLINENSISFELLDLGTKGFELTDELYIKMNARGKQLTA
                     FENFKANFIQLIDKYFKDKKLDHPIKGKISYSGYFSYKIEKEWTDLFWAFRGVKKTTD
                     EGLSNYFEFLTQMLYFKTHKDANADDFKNSFTQYEEVYSTEENLLFLFNSLDKLYDVF
                     CLNGGAKKENINNFFTSIENYTSFFWNTTKGNSLFESIVTNTKNEDARNKISLFCILH
                     YLIKHELSTSNNQLVNYIRIVRNLMQATRQRNETKYNTNIRINFFGSYWMLFEQLSSA
                     DVYNKIQEPTLNNKSTRISDTSLNNEIEKAKILQTNDSNNGALLSIENIEDFGGLIHQ
                     LKPELTKSKLQNYLQAIKEIWSNSISDTLRIQALIACGFDSLYIKDTKMGKTYYFGKR
                     GNWSVILTNDEEEISKSILSLLDTYINNKEKNNKLQTIIDEWLVTNKTDRSYKYYFLK
                     YPEFTSKLNYFVWPNDYEIRMLGTEGSNPLIAYHINPFVLTVCRKINDKNVCDENQCY
                     LQYSGNSPLILKNGVTMTCTKEGWKMPVNS"
BASE COUNT         1651 a          552 c          665 g         1274 t
ORIGIN      
        1 tcgtagaaag ttcagtggtt aaaatccctg cctgcgtata gctgagaacc gttatagcca
       61 atggaaagaa atatgattat aataccagag actaaagcaa tgacagaatt agaaaaccca
      121 agaataagac ccgttggaga tttactccaa gaaaactttt ttgttccacg gtatcaaaga
      181 gggtatcgtt ggggaaaaca ggaaattaca gagctgcttg atgatatttt acagtattac
      241 aatgctactc aaaatagaga aaataaagtt agtaagttct attgccttca accagttgtt
      301 gtaaaaaaga aggagtggtt taataatagc aaagagaaaa ccaaaggttg ggagctgatt
      361 gatggtcaac aaaggcttac aacaatacta ctcattttaa attacttgga agatgtaagg
      421 aagttattaa ataacaatat ggacatttat tctattgatt ttgaaactag agaaaattgc
      481 aaaccatttt ttcaggataa aacctacaaa aaaacggtag atgaaacaaa tgtcgatttt
      541 taccacattt caaaagcata tgagtatatt aacaagtggt ttgaaaagaa taatcataaa
      601 gttgaaatac taaacaccgt actaaaatcg gattacaatg tttcaataat ttggtacgaa
      661 gccgttgaca ataaccaaga taatgacgac agttcaatag agttatttac ccgcttaaat
      721 gatggaaaaa tccctttgac ggatgctgaa ttaatcaaag cattgttgct tcaggcagat
      781 ttatatccaa caaacgagga cagatttgta aagcagcgac tatttgaaat agctaccgaa
      841 tgggacgata tagaagctaa actacaagat gagaaatttt ggcttttttt gaacgacaca
      901 aattatcaac cttcatctaa aatagaattt atttttaaac ttctagctga caaatggaat
      961 gagtttgaga aacaatcgct tgtgaagtat aacttaattg atggcaaacc caagcattat
     1021 gaatttttag tttttgacaa gtatttatca aataaacgag cagcattttc agataaagat
     1081 gaggatggta aagacatatt agaaccagtc aatgagatat ggaaagatat aaaagaaata
     1141 ttcagtatat tttatgaatg gtacaacaac cataatttat ttcattacat aggtttctta
     1201 ttggctattg aaaaaaacaa agaagaactt attcgagatt taatcgatct aaaactaaca
     1261 aaaacagatt ttgaagataa tatcaaaaac agaattgcaa attcagttaa aattaataaa
     1321 aaggacaaag agtctggtat tattaaacag ttgaaccaaa tagcttatgg tgaagataat
     1381 caggaaataa taaaagttct tttgctattt aatgttgatg ctttagttaa acacaaaaag
     1441 gaaaatgcaa aatttccgtt tcacttgtat aaaaaggaga agattacaag catagaacat
     1501 attcatcctc aaaatccacc gagtattgat gcagatgaag acagggcaag aatatggctt
     1561 tctcagcata aaacatcttt gtcttcaatg aaagaatttg ctggaagcaa taaggatgag
     1621 attgataatg tattgcaaca aacagatagt ctattaatta attacgataa agaagttttt
     1681 aaaagtctat ttactcaaac attggatttg tatattaaaa tttctggttt taaagaatct
     1741 gaattgcata cactgtataa tttatcctta gtggataaag acacaaacag ccaactcaat
     1801 aattcttttt ttgatatcaa gagagaaatt ttaaaagaaa ataaattggg tagatatgtg
     1861 cctatttgca ctcaaagggt attttcaaaa tactattcta acagttcaaa agaaatgata
     1921 ttctggaaca atgacgatag acaagcctat tttaaggcaa tagaatctgt ttatctttta
     1981 tttattaact taataggagc atgcaatggc aactaataat acaacatttt ggaatctgat
     2041 aaattcctat aaaataagca tacccattat tcaaagagac tatgctcaag gtcgtgaaga
     2101 agaatttgaa aaacgtgaaa agtttttaaa tgccatcctt aagcatttaa acaatgataa
     2161 aaaactgcat ctagattttg tttatggaag agtaaaagac aatacttttt accccataga
     2221 cgggcaacag cggttgacga ctttattcct attgcattgg tattttgcat taaaggaaaa
     2281 agtcagtagt gaagaaaaat tgaaattaag ccaatttgta tatgatactc gaattagttc
     2341 aagagagttt tgtcaatcat taattaaaga ggatattgaa ttgcctataa ctcctgatga
     2401 tgatgcattt ataactgcta ttaaaaacaa accttggtat agagataaat gggatacaga
     2461 ccctactatt aaagcaatgc ttgtaatgat tcaagacatt cataataaat tttataatat
     2521 taattcacct tcggtattta ctcaactgat taacgaaaac tcaatttctt ttgaattatt
     2581 ggatttaggg acaaaaggat ttgaactaac cgatgagctt tacataaaaa tgaatgcaag
     2641 aggcaaacaa ctcactgctt ttgagaattt taaagcaaat ttcattcaat taattgataa
     2701 gtattttaaa gacaaaaagc ttgaccatcc aattaaaggt aaaatatcct attctggcta
     2761 cttttcttac aaaatagaaa aagagtggac tgatttgttt tgggctttta gaggagtcaa
     2821 aaaaactaca gatgaagggt tatctaatta tttcgagttt ctaacgcaaa tgctttattt
     2881 caaaacacat aaggatgcaa atgctgatga ttttaaaaat agttttacac aatatgaaga
     2941 agtatatagc actgaagaaa acctgctttt tctattcaat agtttagata aactatacga
     3001 tgtattttgc ctaaatggag gagctaaaaa agaaaatata aataatttct ttacctcaat
     3061 tgaaaactac acctctttct tttggaacac aactaaggga aatagtctat ttgaaagtat
     3121 tgttacaaat acaaaaaatg aagatgcaag aaataaaatt tctctttttt gtattttaca
     3181 ttatttgatt aaacatgaac tatcaacttc aaataatcag ctggtaaatt atattagaat
     3241 agttagaaat ttaatgcaag caacgagaca aagaaacgaa acaaaataca atacaaatat
     3301 tcgtattaat ttctttggaa gttattggat gctttttgag caattatcat cagctgatgt
     3361 ttacaataaa attcaagagc caactttaaa caataaaagt actcgaataa gtgataccag
     3421 cttaaataat gagattgaaa aagctaaaat acttcaaaca aatgactcta ataatggagc
     3481 gctattatct attgagaata ttgaggattt tggtggttta atccatcaac tcaaacccga
     3541 attaactaaa tctaaacttc agaattattt acaagcaatc aaagaaatat ggagtaactc
     3601 aatttctgat acattaagaa tacaagcatt aatcgcttgt ggtttcgata gtctttatat
     3661 caaagatact aagatgggaa aaacttacta ttttggaaaa agagggaact ggtcagtcat
     3721 attgacaaat gatgaagaag aaatttcaaa aagtatttta tcattacttg atacctacat
     3781 aaataataaa gaaaagaata ataaattaca aacgattatt gatgagtggt tagttactaa
     3841 taaaacagat aggtcgtata aatattattt tttaaaatat ccagagttta catcaaaact
     3901 aaattatttt gtttggccaa acgattatga aattaggatg ttaggcacag aaggttcaaa
     3961 tcctttaata gcttatcata taaatccttt tgtactaaca gtttgtagga aaattaatga
     4021 taagaatgta tgcgatgaaa accaatgtta tcttcaatat tcaggtaatt cccctttgat
     4081 tttaaaaaat ggagttacta tgacttgcac aaaagaagga tggaaaatgc cagttaattc
     4141 aa
//
LOCUS       DAOQCW010000148         4044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_10957_length_4044_cov_5.484081, whole genome shotgun sequence.
ACCESSION   DAOQCW010000148 DAOQCW010000000
VERSION     DAOQCW010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10957_length_4044_cov_5.484081"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..1314)
                     /locus_tag="PLS16_10560"
     CDS             complement(67..1314)
                     /locus_tag="PLS16_10560"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNJ61568.1"
                     /translation="MISEGYSFIFWLIIIFILFEALLNFILDRRNSQSWLLPIPEILK
                     DVYNQEQYTKAQKYHAESERLAAISSLFGIILTLGFLIFKGFAFVYLWIGTLVDSELL
                     QILLFFGFFMIVSDIISLPFSLYSTFVIEEKYGFNKMSLKTFILDKIKGYALGILIGG
                     LLLSAFVWFYQNTGQLFWIYAWILFTVFSVFMAMFYTSWIVPIFNQLRPLEEGNLRTK
                     IEEFAAKVQFPLTNIFVIDGSKRSTKANAYFSGLGSKKTIVLYDTLIAEHSEDELVAI
                     LAHEVGHYKLKHIQKSLAIGILQMGIVLFVLSWAVSFPQLTSALGLESNKPVFALGLI
                     AFSMLYAPISTLTGLLMNIYSRKNEFEADNYAKENTGSGVFLISALKKLSSNNLSNLN
                     PDKWYVFFNYSHPPLKNRIEKLL"
     gene            1407..1514
                     /locus_tag="PLS16_10565"
     CDS             1407..1514
                     /locus_tag="PLS16_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HNJ61569.1"
                     /translation="MGKGDKRSKRGKIVNGSFGNTRPAKTKAKAAPKKD"
     gene            1568..2695
                     /locus_tag="PLS16_10570"
     CDS             1568..2695
                     /locus_tag="PLS16_10570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ61570.1"
                     /translation="MSESKNTTPLKLAMVLDTYDDARNGAVISTQRFTKLLRENGNEV
                     IVVSTGKEESGKVVLKEFYPPILIVRGVMERMKFIFAWPDKQKLKEVISNVDLVHSHF
                     TFILAFQTIKIAKQLNKPIVSTFHVQAEQIANNVGLTSPWIISFIYKIFIKFFYNKSD
                     LVICPSQFASDEIKRYGCTASTVVISNGVTEEYQEKNLEKHQRFTILTVGRNAAEKRQ
                     EILIQAVANSKHKDKIDIQIIGDGPKRQNLENLANQLLPGQVEFNYLPTQQVIDYYNK
                     AHLYVHCAAVEVECMTAIEAMACGLPIIIAESELSATKQFALNQDYLFKTVTELTSKI
                     DYLFENRNILEEAKVAYLQKVEQYKIKNSYLKLVEAYKSVL"
     gene            2835..>4044
                     /gene="dnaE"
                     /locus_tag="PLS16_10575"
     CDS             2835..>4044
                     /gene="dnaE"
                     /locus_tag="PLS16_10575"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNJ61571.1"
                     /translation="MCDFTPLHCHTQFSLLDGAADIDEMVKKAKEDGMTAVAITDHGN
                     MFGVFKFFAACKKHGIKPIIGCEVYVVEDRFNKKFTRENKDKRYHQLLLAKNEIGYKN
                     LMHIVSLGFIDGLYMDFPRVDFELIKAHSEGLIATTCCVGAEVPQTMIYKGVAEAEKV
                     FLKWLDVFKEDYYIELQRHDIDDLDGTGWSQEAINQELLKFAKKHNVKPIATNDSHYV
                     NQKDYEAHDILLCMQTGKDFIDPNRFKFPNDKFFFKTQYQMKDLFKDVPNSIENTMEI
                     AEKVSSLDLNRDILLPAFQIPPAFKDQDDYLRFLTFDGAKRRWGAILPVEIIDRLDFE
                     LATIKKMGFPGYFLIVQDFIQEGRNLGVSIGPGRGSAAGSAVAFAIGITNVDPIKYNL
                     LFERFLNPDRV"
BASE COUNT         1554 a          628 c          587 g         1275 t
ORIGIN      
        1 atattgcgat ttacattata caccaatttc aacattttaa agaaagaaaa gcaaaattag
       61 tcgctcttat aaaagctttt caatacgatt ttttaatggt gggtgtgaat aattaaaaaa
      121 cacataccat ttatctggat ttaagttact taaattatta ctacttaatt ttttcaaggc
      181 acttattaag aacactccag agcctgtatt ttcttttgca taattatcag cttcaaattc
      241 attttttctt gaataaatat tcatcaataa tcccgtcagt gtagaaattg gtgcatacaa
      301 catactaaaa gcaattaatc ctaaagcaaa aacaggctta ttcgattcta aacctaatgc
      361 acttgttaat tgaggaaatg aaactgccca agataaaaca aacaagacaa ttcccatttg
      421 aagtattcca attgctaaac tcttttgaat atgtttcaat ttataatgac ccacttcatg
      481 tgctaatatg gcgaccaatt catcttcact atgttcagca atcaaagtat catataaaac
      541 tatagttttt ttactaccca aaccactaaa ataagcattc gccttggtag aacgtttgct
      601 accatcaatt acaaaaatat tagtaagtgg aaattgaact tttgcagcaa attcttcaat
      661 ttttgttctt aggttgcctt cttctaatgg ccttaattga ttaaaaatag gaacaatcca
      721 gcttgtataa aacattgcca taaaaacaga aaaaacagta aatagaatcc aagcatatat
      781 ccaaaataat tgtcccgtat tttgataaaa ccatacaaaa gcactcaata atagaccacc
      841 aattaaaatt cctaaagcat aacctttaat cttatctaaa ataaaagttt taaggctcat
      901 tttattaaat ccatactttt cttcaataac aaaagtacta tacaaactaa aaggcaaact
      961 aataatatct gatactatca taaaaaagcc aaaaaatagc aaaatttgta ataactcact
     1021 atccaccaaa gttccaatcc ataagtaaac aaaagcaaat cctttaaaaa ttaagaaacc
     1081 caaggttagg ataattccaa acaacgaaga aatagctgct aatctctcag attctgcatg
     1141 atatttttgt gcttttgtat attgttcttg attataaaca tctttaagaa tttctggaat
     1201 aggcaataac cacgattgac tatttcttct atccaaaata aaattcaata aagcttcaaa
     1261 caatataaaa ataattatca accaaaatat aaaagaataa ccttctgaaa tcatagcaca
     1321 aaactattct atttttaatt ataatttagc atactaatta tttatattta aagaaaatca
     1381 ctaattttgc ataaaaataa aataatatgg gaaaaggaga taaacgctca aagagaggta
     1441 aaattgtaaa tggaagtttt ggaaatacaa gacctgctaa aactaaagca aaagcagctc
     1501 ccaaaaaaga ttaatagatt aaacgctttt tattaagtat ttcaagaata tttagaaaaa
     1561 atctataatg tccgaaagta aaaatacaac accgctcaaa ttggcgatgg ttttggatac
     1621 ctacgacgat gctcgtaatg gagccgttat ttctacacaa agatttacaa agttgctcag
     1681 agaaaatggc aatgaggtta ttgtggtatc tactggcaaa gaggagtcag gtaaagttgt
     1741 cttaaaagaa ttctatccgc ctattctaat agtaagagga gtaatggagc gtatgaaatt
     1801 catttttgct tggccagata aacaaaaact aaaggaagta attagcaacg tagatttagt
     1861 tcatagccac tttacattta ttttagcctt tcaaaccatt aagatagcaa agcaactcaa
     1921 caaacctatt gtttcaactt ttcacgtaca ggctgaacaa atagcaaata atgtaggact
     1981 cacaagtcca tggataatat cttttatcta taaaatcttc attaagtttt tttacaataa
     2041 atcagattta gtaatttgtc ctagtcaatt tgcgtcagat gaaataaaaa gatatggctg
     2101 tacagcatct acagtagtta tatctaatgg tgtaactgaa gaatatcaag aaaaaaattt
     2161 agaaaagcat caacgtttta ctatccttac cgtaggaaga aatgctgccg aaaaaaggca
     2221 agaaatttta attcaagccg tagcaaattc taaacacaaa gacaaaattg atattcaaat
     2281 aattggtgat ggtcctaaaa gacaaaattt agaaaatcta gccaaccaat tattaccagg
     2341 acaagtagaa ttcaattatc ttccaacaca acaagtaata gattattata acaaagcaca
     2401 tctatacgta cattgtgccg ctgttgaagt agaatgtatg actgcaattg aagcaatggc
     2461 ttgcggttta cctattatca ttgccgaatc tgaattaagt gccacaaaac aatttgctct
     2521 caaccaagat tacctattta aaacagtaac agaactaact tctaaaattg attacctctt
     2581 tgaaaataga aatattttag aagaagccaa agttgcttat ttacaaaagg tagaacaata
     2641 taaaatcaaa aattcttacc taaaattagt tgaagcctat aaaagtgttt tgtaaacaca
     2701 ttaaattcaa ttatatttta tttttttata attgagtaat tatttctcca cattgaaaca
     2761 atttaacaaa tgtaattgag taagtaattt gttaatgagt atatttactt ttcactttca
     2821 aaaattctaa aactatgtgt gatttcactc cgttacactg ccatactcaa ttttcacttt
     2881 tagatggtgc cgcagatatt gatgagatgg taaaaaaagc caaagaagat ggtatgactg
     2941 ccgttgcaat tacagatcat ggtaatatgt ttggtgtatt taagtttttt gcggcatgta
     3001 aaaaacatgg tattaaacca attatcggat gtgaagttta tgtagtagaa gaccgtttca
     3061 ataaaaaatt tactcgagaa aataaagaca aaaggtatca tcaattatta ttagcaaaaa
     3121 atgaaattgg ttataaaaac cttatgcata ttgtttcctt aggttttatt gatggcttat
     3181 atatggattt ccctcgtgta gattttgaat taataaaagc acatagtgaa ggtttaattg
     3241 ctacaacttg ttgtgttgga gctgaagttc ctcaaactat gatatacaaa ggtgtagcag
     3301 aagctgaaaa agtattctta aaatggctag atgtttttaa agaagattat tatatcgaac
     3361 tgcaaaggca cgatattgat gatttagatg gaactggttg gagtcaagaa gccattaatc
     3421 aggaattatt aaaatttgca aaaaaacaca atgtaaaacc aatcgctacg aatgattctc
     3481 attatgtcaa ccaaaaagat tatgaagcac atgacatttt actttgtatg caaactggta
     3541 aggattttat agatccaaat cgttttaaat tccctaatga caaattcttc tttaagacac
     3601 aatatcaaat gaaagatttg tttaaagatg taccaaattc catagaaaac acgatggaaa
     3661 ttgccgaaaa ggtgagttca ttagatttga atagagatat tttattacca gctttccaaa
     3721 ttccaccagc atttaaagac caagacgatt atttacgttt ccttacattt gatggagcaa
     3781 aaagacgatg gggtgcgata ttacccgtcg aaattataga tcgcctagat tttgaattag
     3841 caaccataaa aaaaatgggt ttccctggct attttttaat cgttcaagat tttatacaag
     3901 aaggtagaaa cttaggggtt tctatcggac ctggcagagg ttcggcggca ggttcagcag
     3961 tagcttttgc tattggaatt accaatgttg acccaattaa atataatcta ctttttgaac
     4021 gtttcttaaa tccagataga gttt
//
LOCUS       DAOQCW010000149         3970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_11339_length_3970_cov_5.929757, whole genome shotgun sequence.
ACCESSION   DAOQCW010000149 DAOQCW010000000
VERSION     DAOQCW010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11339_length_3970_cov_5.929757"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PLS16_10580"
     CDS             <1..271
                     /locus_tag="PLS16_10580"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNJ61572.1"
                     /translation="AAQEQLINAVTSSDDDFDSKAYDQLTAQITTRELEILQHIALGL
                     TSQDIANKLFISKNTVETHRKNMLSKLNVNNTAALLKIAYKKGLV"
     gene            346..1314
                     /locus_tag="PLS16_10585"
     CDS             346..1314
                     /locus_tag="PLS16_10585"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HNJ61573.1"
                     /translation="MKQIKIDTFLLLTFVTILISYCFPQLGNKAYKGILDNIISIGIA
                     FIFFFYGLKLSIEKIKTGLKNLKLHFVIQITTFVFFPLIIILFYPYFKGENYFYTWLS
                     FLFLAALPSTVSTSVVMVSIAKGNLPAAIFNASISGLIGVFITPIWMGFFILNSDVDY
                     NLSHIYLKLIIEILIPVVLGLSLQKKNGNYVMKHIQLLNKFDKSVILLIIYKSFAESF
                     EQKIFSEVGTMYLLIVTFLCCLLFYLVYFFTGWVAEFLNFNREDTITAQFCGTKKSLV
                     HGTVFSKILIPATIPMGIILLPIMIFHAFQIFVITFISERKSKEIE"
     gene            complement(1358..>3970)
                     /locus_tag="PLS16_10590"
     CDS             complement(1358..>3970)
                     /locus_tag="PLS16_10590"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNJ61574.1"
                     /translation="PSEIEQKWYQHWQNKNYFHSEPDERVPYTIVIPPPNVTGVLHMG
                     HMLNNTIQDVLIRKARMQGKNACWVPGTDHASIATESKVVAMLKEQGIEKKDLTRTEF
                     LKHAWEWKEKYGGIILKQLEKLGASCDWQRTKFTMDESLSEAVIDIFIDLYNKGQIYR
                     GVRMVNWDPKGKTAVSDEEVIHKEVNSKLYYIKYLIEENGQISDQYITVATTRPETIF
                     GDTGVCVHPDDERYKHLKGKRAIVPIIKRSVPIIFDDYIDIEFGTGTLKVTPAHDIND
                     YNLGIKHQLESIDILNDDGTLNEKAQFYIGEDRFAVRKKVAIELEEANLLEKVEDIKN
                     KVGYSERTDAVIEPKLSLQWFLKMDKISKPALENVLNNNIQLTPEKFKNTYKHWMENV
                     HDWCISRQLWWGHQIPAWHDTKGNFVVAKTQNEALEIYKNKFKELFNDQTQLTQDEDV
                     LDTWFSSWLWPISVFDGFKNPEGKDINYYYPTNDLVTAPEILFFWVARMIIAGYEYRN
                     EKPFSNVYLTGIVRDKQGRKMSKSLGNSPDPIDLIEKYGADGVRTGMLFSSPAGNDLP
                     FDEKLCEQGRNFTNKIWNALRLIKNWEIDETSASKIDLTTAFEWFENKLAQTIKTTNE
                     LYSQYRLSEVLTTLYTFIWDDFCSWYLEYIKPSFGEKIDAKSYQKTVGYFEEILKLIH
                     PFMPFITEEIYHLLDENRASDIIVAPYPNERNFDVNKIATGEKMKTFIANVRDVRNRN
                     GLSPKQTLDVYVQTKDKVFYEVCNDILLKISNINPINYTDTNIENAITNLVDKDKVFI
                     VTGIEINTDEEKEKLKKEILYYEGFITSIEKKLNNERFVQNAKPEIVEIERQKLKDGQ
                     TKLQAFKESLSKLG"
BASE COUNT         1199 a          627 c          581 g         1563 t
ORIGIN      
        1 tgctgctcaa gaacaattaa tcaatgcagt tacttcatct gatgatgatt ttgattctaa
       61 agcttacgac caattaacgg cacaaattac aaccagagaa ttggaaatat tacaacatat
      121 cgcattaggt ttgacgagtc aagatattgc aaataaattg tttatcagta aaaatacagt
      181 agagacgcat cgtaaaaata tgctttctaa attgaatgtc aataatacag cagcattatt
      241 gaaaattgcc tataaaaaag gattggttta gtttgttaca ttctgtaatt ataaaataat
      301 aaaatcgttt aggttttagt ttttctttat ttgatatttg ataaaatgaa gcaaattaaa
      361 atagatactt ttttgttact aactttcgta acaattttaa tttcgtattg ctttcctcaa
      421 cttgggaata aagcctataa aggaatttta gataatatta taagcattgg gattgccttt
      481 atctttttct tttatggatt gaaattaagt atagagaaga taaagacagg gttaaagaat
      541 ttgaaattac atttcgttat tcaaataacg acttttgtat tttttccatt gataattatt
      601 ttgttttatc catactttaa aggagaaaat tacttttata catggctttc atttttgttt
      661 ttagctgcat tgccctcaac ggtctcaact tcagttgtga tggtttctat cgcaaaagga
      721 aatttaccag ctgctatttt taacgcaagc atctctggat taattggagt tttcataacg
      781 ccaatttgga tgggcttttt tatcttgaat agtgatgttg attataattt gagccatatt
      841 tatttaaaat taataataga gattttaata cctgttgttt taggtttgtc tttgcaaaaa
      901 aaaaatggta actatgtaat gaaacatata caattattga ataaatttga caagtcggtt
      961 attcttttaa ttatatataa aagctttgca gaatcatttg agcagaaaat attcagcgaa
     1021 gttggaacga tgtatttgct tatagttact ttcctttgtt gtttgctatt ttatctggtt
     1081 tatttcttta caggttgggt tgctgaattt ttaaatttca atagagaaga tactattaca
     1141 gctcaatttt gtggtactaa aaaatcattg gtgcatggta cagttttttc taaaatactt
     1201 attccagcaa ctattccaat gggtattata ctgttgccca taatgatatt tcacgccttc
     1261 caaatttttg ttataacctt tatttccgaa agaaaatcta aagaaataga ataaagatta
     1321 actagcttcg gtcttatagc ataaaaaatt atactattta gcctaatttt gataaacttt
     1381 ctttaaaagc ctgaagtttg gtttgaccat ctttaagctt ttgtctctct atttctacaa
     1441 tttcaggttt agcattttgt acaaatcttt cattatttag ctttttttca atcgatgtga
     1501 taaagccttc ataatataga atttcttttt taagtttttc tttttcttcg tctgtattga
     1561 tttctatgcc agtaacgatg aaaactttgt ctttgtcaac taaatttgta atggcatttt
     1621 ctatattagt atcagtgtag tttattggat ttatattgct gatttttaat aatatatcat
     1681 tgcatacttc atagaacact ttatcctttg tttgtacata aacatctaaa gtttgttttg
     1741 gagaaagtcc atttctattt cttacatcac gaacattagc tataaaagtt ttcattttct
     1801 cgccagttgc aattttgttt acatcaaaat ttctttcgtt gggatatgga gcaacaatga
     1861 tatcacttgc tctattttca tctaataaat gataaatttc ttccgtgata aaaggcataa
     1921 aaggatgaat gagtttcaag atttcttcaa aatagccaac tgttttttga tagctttttg
     1981 catctatctt ttcgccaaaa gaaggtttga tgtattctaa ataccaagaa cagaaatcat
     2041 cccaaataaa tgtataaagt gtggtcaata cttctgacaa tctgtattga ctgtacaatt
     2101 cattcgttgt tttaatggtt tgtgctaatt tattttcaaa ccattcaaaa gctgttgtta
     2161 aatcgatttt tgaagctgaa gtttcatcaa tttcccaatt tttaattaaa cgaagtgcgt
     2221 tccaaatttt attagtaaag tttctgcctt gttcacatag tttttcatca aatggtaagt
     2281 cgttgccagc aggtgaggag aatagcattc ctgttctaac gccatctgca ccatatttct
     2341 caattaagtc aattggatcg ggtgagttgc ctaatgactt agacattttt cgtccttgtt
     2401 tatctcgtac aattccagtt agatatacat ttgaaaatgg cttttcattt cgatattcgt
     2461 aaccagcaat aatcattctt gccacccaaa agaataaaat ttctggtgca gtaactaaat
     2521 cgttggtagg ataataataa ttaatgtctt ttccttcagg atttttaaag ccatcaaaaa
     2581 cagaaattgg ccatagccaa gaagaaaacc aagtatctaa aacatcttca tcttgagtaa
     2641 gttgcgtttg gtcattaaat agttccttga acttgttttt ataaatttct aatgcttcat
     2701 tttgtgtttt tgctaccaca aaatttcctt tcgtatcgtg ccaagctgga atttgatgtc
     2761 cccaccacaa ttgtctagaa atacaccaat cgtgtacatt ttccatccaa tgtttatagg
     2821 tatttttaaa tttctcagga gttaattgaa tattattatt caatacattt tctaatgcag
     2881 gtttggatat tttatccatt ttcaaaaacc attgcaacga taattttggt tctattactg
     2941 catctgttct ttcggaatag cctactttat ttttaatatc ttctactttt tctaataagt
     3001 tggcttcttc taattcaatg gcaacttttt ttctaacagc aaatctatct tcgccaatat
     3061 aaaattgtgc tttctcattt aaagtaccgt catcattcaa tatatctata gattctagtt
     3121 gatgttttat tcctaaatta tagtcgttaa tgtcgtgtgc aggtgtaact ttcagtgttc
     3181 cagtaccaaa ttcaatatca atataatcat caaaaattat aggaacgctt ctcttaatga
     3241 ttggcacgat tgctcttttt ccttttaaat gtttatatct ttcgtcgtcg ggatgcacac
     3301 aaacgcccgt atcaccaaaa atagtttctg gtcgtgttgt ggctacagta atatattggt
     3361 cgcttatttg tccgttttct tcaattaaat atttgatata atatagttta gaattcactt
     3421 ctttgtgaat gacttcctcg tctgaaacag cagtttttcc ttttggatcc caattaacca
     3481 ttcgtacgcc acgataaatt tgacctttgt tgtataaatc tataaatata tcaattacag
     3541 cttcagacaa actttcgtcc attgtgaatt ttgttctttg ccaatcacaa gaagctccaa
     3601 gtttttctaa ttgttttaag atgattccac catatttttc tttccattcc caagcatgct
     3661 ttaaaaattc tgtacgtgtc aaatcttttt tttctattcc ttgttctttc agcattgcaa
     3721 cgactttaga ttctgttgca attgaggcgt gatctgtacc aggaacccaa catgcatttt
     3781 ttccttgcat acgagctttt cggattaata catcttgaat tgtattattt agcatgtgtc
     3841 ccatgtgtaa tacacctgtt acatttggcg gtggaataac aattgtatat ggcactctct
     3901 cgtcgggttc agaatgaaaa tagtttttat tctgccaatg ttgataccat ttttgttcaa
     3961 tttctgatgg
//
LOCUS       DAOQCW010000150         3936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_11523_length_3936_cov_6.240660, whole genome shotgun sequence.
ACCESSION   DAOQCW010000150 DAOQCW010000000
VERSION     DAOQCW010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11523_length_3936_cov_6.240660"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="PLS16_10595"
     CDS             <1..1080
                     /locus_tag="PLS16_10595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNJ61575.1"
                     /translation="LSTWSNFPYFFTETLKEKGIKVNYVNIQPNKFLKNIYNFTIDKG
                     LKKTKQHNAFDYYRTPINRFFANKIIQSAIKKFPNADANLFLNFSFSAKPYSNKPTIL
                     FGDWTYEHYFPHFKKRKPGKLEQTFIDAENQCIENADAVFVMFPKMAEIIKNRYNNKN
                     IFYLGGYFINAKVEKNAETILAKKTNSNKILFIGTIKYKEGVLTLIEAVEILRQQNHH
                     LEIHIIGMKKSDFSKLPDDVFCYGYLDKAKAKDLHIYNELMESAKMIVNTTPKWSGFS
                     SVIEAMSFYNPIITIPYDDFIETFDKKINFGIYNQSPETLADDILNIIQDKNYATLCQ
                     NAHLAVKDFTWSNYIDRFLSKLAEI"
     gene            1107..2033
                     /locus_tag="PLS16_10600"
     CDS             1107..2033
                     /locus_tag="PLS16_10600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNJ61576.1"
                     /translation="MKISVVIPVHNEEGNIFPLYQELLNTIQKIGTAYEFIYIDDGST
                     DLSYRNIQILAEQDKNVKYIHFSKNFGHQNAVFAGLEKSNGDAIVIIDADLQDPPQLI
                     EKLYQKFIEGYEVVFAQRTYRSDETWLKLASAKLFYRFINKLSDVYIPEDTGDYRMIS
                     KKVKDVICSMPEQNKFLRGQIAWAGFKQVGVPFERQGRRSGKTNYSYSQMFKFAYDGI
                     VSFSNRPLHLATYVGFLISLTAFAVIIYSVYQKYWNNNTVQGWTSIMLSVLFLGGVQL
                     ICLGIIGEYIGRILDNVKDRPNYVIKESNFED"
     gene            2159..2386
                     /locus_tag="PLS16_10605"
     CDS             2159..2386
                     /locus_tag="PLS16_10605"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
                     /protein_id="HNJ61577.1"
                     /translation="MGKYVYQHHEYDNWAEFITIKIKLAQTNAAIKVNSEMLTLYWEI
                     GYSILEKQNKNGWGSKVIDLLAVDLAKNFPQ"
     gene            2418..2642
                     /locus_tag="PLS16_10610"
     CDS             2418..2642
                     /locus_tag="PLS16_10610"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
                     /protein_id="HNJ61578.1"
                     /translation="MRAFAEAYPEFPFVQIPLAQSQDEFVQVPLAQITWYHHISLLTK
                     VKDTAARAFYIAETAKNEWSRDVMLLQIQN"
     gene            2931..3341
                     /locus_tag="PLS16_10615"
     CDS             2931..3341
                     /locus_tag="PLS16_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNJ61579.1"
                     /translation="MVYLTRRETFNAAHKLYNNQWTVEQNAEVFGKCANKNWHGHNYE
                     LFITVKGKPNLDTGFIMNVKDLSRLIKKEITEKLDHKNLNLDVDIIPKTMMPSTENLA
                     IIIWNILNPLITDAQLHCVKLVETENIFVEYYGE"
     gene            3347..3790
                     /locus_tag="PLS16_10620"
     CDS             3347..3790
                     /locus_tag="PLS16_10620"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNJ61580.1"
                     /translation="MHFILKSFEELNAHQLYAILQLREEVFIIEQNVVYKDLDDKDQK
                     AKHLLGFYEDKLVAYARLIPDGISYPDFTSFGRVVVAPKLRNTKFGILLMQEVMQKIE
                     LLYPEMPIKISAQSYLIPFYEKFGFVVNSAEYIEDHLPHTEMVKK"
     gene            3845..3929
                     /locus_tag="PLS16_10625"
     tRNA            3845..3929
                     /locus_tag="PLS16_10625"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3879..3881,aa:Ser,seq:tga)
BASE COUNT         1479 a          542 c          609 g         1306 t
ORIGIN      
        1 ctaagtactt ggtctaattt tccttatttc tttactgaaa cattgaaaga aaaaggcata
       61 aaagtaaatt atgtcaatat ccaacctaat aaatttttaa aaaatattta taattttact
      121 attgacaaag gtttaaaaaa aaccaaacag cacaatgctt ttgattatta tagaacacct
      181 atcaatcggt tttttgcaaa taaaatcatt caatctgcga taaagaaatt tccaaatgca
      241 gatgccaatt tatttctaaa ttttagcttt tcagcaaaac catattctaa taaaccgact
      301 atattatttg gcgattggac ttatgagcat tatttccctc attttaaaaa aagaaaacca
      361 gggaaattag agcaaacctt tattgatgct gagaatcaat gtattgaaaa tgctgatgcc
      421 gtatttgtaa tgtttccaaa aatggcagaa attataaaaa accggtataa caacaaaaat
      481 attttctatt tgggcggtta ttttatcaat gccaaagtag aaaaaaatgc agaaactatc
      541 ttagctaaaa aaacaaattc caacaaaatt ctttttattg gaacgataaa atataaagaa
      601 ggtgttttga cattaatcga agctgttgaa atactccgac aacaaaatca tcacttagaa
      661 attcatataa taggcatgaa aaaaagtgac ttttctaaat tacccgatga tgtattttgt
      721 tatggttatt tagataaggc aaaagccaaa gacttgcata tctataacga attgatggaa
      781 tctgctaaaa tgatagtcaa tacaacaccc aaatggagtg gcttttcttc tgttattgag
      841 gctatgagtt tttataatcc tattattaca attccttatg atgattttat agaaactttt
      901 gataaaaaaa taaattttgg catttataat caatcgccag aaacattagc tgatgatatt
      961 ttaaacatta tccaagataa aaattatgct acattatgtc agaacgcaca tcttgccgta
     1021 aaagatttta catggagcaa ttatatagat agatttttgt cgaaattagc tgaaatctaa
     1081 attttttatt tattaatttt agcttgttga aaatctctgt tgtcatacca gtacataacg
     1141 aagaaggaaa tattttccct ttgtatcaag aactattgaa tactatacaa aaaattggta
     1201 cggcttatga atttatatat attgatgatg gcagtacaga tttatcttat cgaaatattc
     1261 aaattctagc tgagcaagat aaaaatgtaa aatatattca ttttagtaaa aattttggtc
     1321 atcaaaatgc tgtttttgct ggtttagaaa aatcaaatgg cgatgctatt gttattatag
     1381 atgctgacct gcaagaccct ccacaattga tagaaaagtt gtatcaaaaa tttatagaag
     1441 gatatgaagt tgtttttgca caaagaacct atcgctctga tgaaacgtgg ctaaaacttg
     1501 cttctgccaa acttttttat cgattcatca ataaattatc tgatgtttat attcctgaag
     1561 atactggcga ttatagaatg atttcaaaaa aagttaaaga cgtaatttgt agtatgccag
     1621 agcaaaataa atttttgcgt ggtcaaattg cttgggctgg atttaaacaa gtaggcgttc
     1681 cttttgaaag acaaggcaga cgaagtggca aaaccaacta ttcctattcc caaatgttta
     1741 aatttgctta cgatggtatc gtctcttttt ctaatcgtcc attacatttg gcaacttatg
     1801 taggattttt aatttcatta actgcttttg cggttatcat atattctgta tatcaaaaat
     1861 attggaacaa taataccgtt caaggttgga cttcaattat gcttagtgtt ttatttttag
     1921 gtggcgttca attaatttgt ttagggatta ttggtgaata tattggtaga attttagata
     1981 atgtaaaaga tagaccaaac tatgtaatca aggaaagtaa ttttgaagat taggaatgtt
     2041 taattcttgt tatagctagt tttgtcaatc ataaaccttt aattagacac caatcaatta
     2101 gttattataa ctaacaaaaa atatgtaaga aaggtactaa aaaacagata actctaaaat
     2161 gggtaaatac gtttatcaac atcatgaata cgataattgg gctgaattta ttactataaa
     2221 aataaaactt gctcaaacga atgccgctat taaagtaaat tcagaaatgc ttacacttta
     2281 ttgggaaatt ggatattcta ttcttgaaaa acaaaataaa aacggttggg gtagcaaagt
     2341 gattgatctt ttagccgttg atttagctaa aaacttcccc caataattca ggcttttctg
     2401 taaggaatct aaaatatatg agagcttttg ccgaagctta tccagaattt ccatttgtgc
     2461 aaattccact tgcacaatca caagatgaat ttgtgcaagt accacttgca caaataacgt
     2521 ggtatcatca cataagccta ttaactaaag taaaagacac agcagcaagg gcattctaca
     2581 ttgcagaaac agctaaaaat gaatggagta gagatgtgat gttattacaa atacaaaatt
     2641 aactttatga aagagatgga aaagccttaa ataattttga aacaacatta ccagactatc
     2701 aatctgattt agctaaaagt attttcaaag acccttacaa tttcgatttt ttaatgcttt
     2761 ctagaaaagt tagtaaaaga aaatattgat aaatttgaac agttagaaga tatagaacag
     2821 tagaaataaa atccacttta tttttatttt atagaattct cataattaaa attaataaaa
     2881 gtactaagaa tttaacacta tgtaattatt tgaatactta aatttgtttt gtggtttatc
     2941 taacaaggag agaaactttt aatgctgccc ataaattata caataatcaa tggacagttg
     3001 aacagaatgc tgaagttttt ggaaaatgtg ctaataaaaa ttggcacgga cataattatg
     3061 aattatttat aaccgtaaaa ggcaaaccca atttggatac tggtttcatt atgaatgtca
     3121 aagatttaag taggcttata aaaaaggaaa taacagagaa attagaccat aaaaatttga
     3181 atttagatgt cgatattatt ccaaaaacta tgatgccatc aacagaaaac ttagctatta
     3241 ttatttggaa catcttaaat ccattaatca cagatgcaca attgcattgt gtgaaattgg
     3301 tagaaacaga aaacatattt gtagaatatt acggagaata aattatatgc actttatatt
     3361 aaaatcattt gaggaactca atgcacatca gttgtatgct attttacaac tcagggaaga
     3421 agtttttatt attgaacaaa atgttgtcta taaagattta gacgacaaag accaaaaagc
     3481 aaaacatctt ttaggatttt acgaagataa attagtggct tatgctcgtt taatacctga
     3541 tggtatttca tatcctgatt ttacttcatt tggaagagtt gttgttgctc caaagttaag
     3601 aaacacaaaa tttgggattt tattgatgca agaagtcatg caaaaaatag aattgttata
     3661 tccagaaatg cctatcaaga ttagtgcaca atcttattta attccttttt acgaaaaatt
     3721 tggatttgtt gttaatagtg ctgaatacat tgaagaccat ttgccacata ctgaaatggt
     3781 gaaaaaataa gtaagcaaca aaaatttaat ttacaataat aaaaaatatt ctatctttgc
     3841 actcggagag atggcagagc ggtttaatgc ggcggtcttg aaaaccgttg actgtaacag
     3901 gtccgggggt tcgaatccct ctctctccgc tccata
//
LOCUS       DAOQCW010000151         3919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_11618_length_3919_cov_4.335663, whole genome shotgun sequence.
ACCESSION   DAOQCW010000151 DAOQCW010000000
VERSION     DAOQCW010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11618_length_3919_cov_4.335663"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PLS16_10630"
     CDS             <1..317
                     /locus_tag="PLS16_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61581.1"
                     /translation="SVGAYIGYGARYRLHHLAVGARGSFHWWQLLDDKVSADLKSDKI
                     DIYMPVHLGVYMYMGGGTTARFNGGAGFGFRYYFIDNIGVNVEFGRQEMSWAKLGFQF
                     KL"
     gene            417..2012
                     /gene="nadB"
                     /locus_tag="PLS16_10635"
     CDS             417..2012
                     /gene="nadB"
                     /locus_tag="PLS16_10635"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF006567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNJ61582.1"
                     /translation="MYDFLVIGSGIAGLSFALKVADKFPNKKILILTKANEDDSNTKY
                     AQGGIAVVTDFDVDNFEKHIEDTHIAGDGLCNPNVVDFVVREGRQRVDELIKWGTQFD
                     KEDNGTYKLGREGGHSEFRVLHHKDVTGREIERALLQKVHETPNIEIHQHFFVTDLIT
                     QHHLGRMITRATTNNQCFGVYALNKDTNQIEKIISKITVLATGGFGQVYRSTTNPTIA
                     TGDGIAMVYRAKGKVSNMEFVQFHPTALYEPDVSPSFLITEAVRGDGGILKTKGGEEF
                     MKKYDKRESLAPRDIVARAIDNEMKISGEACMYLDCRHMDFDNFIHHFPNIYEKCKSI
                     GIDVQKDMIPVVPAAHYACGGIRVNEFGQTSINNLYACGECSSTGLHGANRLASNSLL
                     EAIVYSHRIAEHAIQQVDNIEVDEKRFQEWDTTGTSHPKEMVLITQSLRELKDIMSNY
                     VGIVRNNVRLKRASERLYLLFKECEDMYNTTVISPQLMEVRNLITIGYLITRSALLRK
                     ESRGLHYTTDYPNHSEFTEETVL"
     gene            complement(2045..2200)
                     /locus_tag="PLS16_10640"
     CDS             complement(2045..2200)
                     /locus_tag="PLS16_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61583.1"
                     /translation="MKGLKASFSIKILQDGGHYPMETTALAQLTKYTDEFIQSIEQLD
                     KEKNSIL"
     gene            complement(2354..>3919)
                     /gene="recG"
                     /locus_tag="PLS16_10645"
     CDS             complement(2354..>3919)
                     /gene="recG"
                     /locus_tag="PLS16_10645"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNJ61584.1"
                     /translation="QLKPENIHENIPEYVLKHYHLISRFEAYKQIHIPQNIDDQNNAT
                     KRLKFEELFFIQLNLLKNKVIRKKEKGILFEKIDTYFNNFYNNNLPFDLTNAQKRVLK
                     EIRKDTLSGLQMNRLLQGDVGSGKTIIALLSMLMAFDNNYQAALMAPTEILAQQHFQN
                     IQEVLNGMDVKIELLTGSTKTKQRKIILEQLKNGELNGIIGTHALIEDQVIFKNLGLA
                     VVDEQHRFGVEQRAKLWKKNTIVPHILVMTATPIPRTLAMTLYGDLDVSVIDELPPGR
                     KAIKTVHRYESHRIAVFDFMRDEIKKGRQIYIVYPLIAESEKLDLIDLTNGYESICRA
                     FPMPEYHVSIVHGKMKPDDKNFEMDRFVKGITQIMVATTVIEVGVNVPNASVMIIENA
                     DRFGLSQLHQLRGRVGRGAEQSFCILMTDFKLSSDAKTRINTMVETNDGFKIAEVDLK
                     LRGPGNIEGTQQSGTLDLRIANLATDGIILEEARKVAMNILEQDENLELPEHYPLKVF
                     IQQKRQYNWSNIS"
BASE COUNT         1253 a          606 c          677 g         1383 t
ORIGIN      
        1 caagtgttgg tgcttatata ggttatggag caagatatag gttacatcat ttagcagttg
       61 gagctagagg gtcattccat tggtggcaat tattagatga caaagtaagt gcagatttga
      121 agtcagacaa aatagatatt tatatgccag tacatttagg agtttatatg tatatgggag
      181 gaggaactac tgctagattc aatggtggtg cagggtttgg ttttagatac tattttattg
      241 ataatattgg agtaaatgta gaatttggtc gacaagaaat gagttgggca aaactaggtt
      301 tccaatttaa gttgtaatca atattcttat aaatttaatt atagcaacct ctcttgaggt
      361 tgctttttta atttttttac tgaatttttc aatcaaatca ttacatttgt ttttctatgt
      421 acgatttttt agtaattggt tcaggaatag ccggtctgag tttcgctttg aaagtagcag
      481 ataaatttcc aaataagaaa atattaattc ttacaaaagc caacgaagat gatagcaata
      541 ctaagtatgc acaaggtgga attgcagtag taaccgattt tgatgtcgat aattttgaaa
      601 aacatattga agatacacat attgcaggcg atggtttatg caatccaaat gtagtagatt
      661 ttgtagttcg tgagggtcgg caacgtgttg atgagcttat taaatgggga acacaatttg
      721 ataaagaaga taatggcact tataaacttg gcagagaagg tggacattct gaatttagag
      781 ttttacatca taaagatgta acgggtcgag aaatagaaag agccttattg caaaaggttc
      841 acgaaacgcc taatattgaa atacatcaac atttctttgt tactgattta attactcaac
      901 atcatttagg aagaatgatt actagagcaa caactaataa ccaatgtttt ggcgtttatg
      961 ctctaaataa agataccaat caaattgaaa aaataattag caaaattacc gttttagcaa
     1021 caggtggctt tgggcaggtt tatagaagca caaccaaccc aacgattgct actggtgacg
     1081 gaattgctat ggtttataga gctaaaggaa aggtttctaa tatggaattt gtgcaatttc
     1141 acccaactgc attgtacgaa ccagatgtaa gtccaagttt tttaattact gaagctgttc
     1201 gaggagatgg cggtattttg aaaacaaaag gtggcgaaga gtttatgaaa aaatatgaca
     1261 aacgtgaatc acttgcacct cgagatattg ttgcaagagc aatagataat gaaatgaaaa
     1321 ttagcggtga ggcgtgtatg tatttagatt gtcgccacat ggatttcgat aattttattc
     1381 atcatttccc aaatatttat gaaaaatgta aaagtatagg tattgatgtc caaaaagata
     1441 tgattccagt tgtgcctgct gctcattatg cttgtggagg aatcagagta aatgagtttg
     1501 ggcaaacttc tataaataat ttatatgcgt gtggcgaatg ttctagtact ggtttgcatg
     1561 gtgccaaccg tttagcgtct aattcattat tagaagcgat tgtttattct catagaattg
     1621 ccgaacatgc tatacaacaa gtcgataata tcgaagtcga tgagaaaaga tttcaagaat
     1681 gggatactac gggaactagt catcctaaag aaatggtatt aattacacag agtttacgag
     1741 aactcaaaga tattatgagt aactatgtgg gtattgttcg taataatgtc cgattaaaac
     1801 gagcttctga gcgattgtat ttattattta aggaatgtga ggatatgtat aatacaacgg
     1861 taatttcgcc tcaattaatg gaagtaagaa atttaattac aatcgggtat ctaattacga
     1921 gaagtgcttt gcttagaaaa gaaagtcgtg gtttgcacta caccactgat tatccaaatc
     1981 actcagaatt tacagaagaa acggtgttgt aagaattact ataatcttat ttctaaatac
     2041 aaaattacag aatactattc ttttctttat ccaattgctc tattgattgg ataaactcgt
     2101 cagtatattt tgttaattga gctaatgccg tagtttccat tgggtaatga ccgccatctt
     2161 gtaaaatttt tatagaaaat gaggctttta atcctttcat agaaatctca cttaaaaatt
     2221 cttttctaaa attagcacct cacttttttg tcctaaattt taaataaaat cattgtttat
     2281 cggcttcggt aacttttcta tcaataattc taaatattga gacttgtcca tcatttttat
     2341 ataattaaaa attctaagaa atattgctcc aattatattg ccttttttgt tggataaaaa
     2401 cttttaatgg ataatgttct ggcagttcta ggttttcatc ttgttctaaa atattcatag
     2461 caactttcct agcttcctct aaaataattc catcagtggc taaattcgca attcgtaaat
     2521 ctagtgtgcc actttgttgt gtgccttcta tatttccagg gccacgtaat tttaaatcga
     2581 cttcagcaat tttgaatcca tcattggttt caaccatcgt gttaattcta gttttagcat
     2641 ctgaacttaa tttaaaatca gtcattaata tacaaaaaga ttgttcggca cctctgccca
     2701 cacgtcctcg caactggtgc aattgagaca agccaaatct gtccgcattt tctattatca
     2761 tcacacttgc attcggcaca ttaacaccga cttcaataac tgtggttgcc accataattt
     2821 gtgttattcc ttttacaaaa cgatccattt caaagttctt atcatctggt ttcattttgc
     2881 catgcactat actcacgtga tattctggca ttggaaaagc acgacaaata ctttcataac
     2941 cattcgtcaa gtcgattaaa tccagtttct cgctttctgc aattaatgga taaacaatat
     3001 aaatttgtct gcctttttta atttcatcac gcataaaatc aaaaactgca attctatgtg
     3061 attcatagcg atgtactgtt ttaatagctt ttcttcctgg tggcaattca tcaattacag
     3121 aaacgtctaa atcaccataa agtgtcatcg ccaatgtccg tggaattggg gtcgctgtca
     3181 ttactaaaat atgcggcaca attgtatttt tcttccaaag tttagctctt tgttctactc
     3241 caaaacgatg ttgttcatca accacagcta atcctaaatt tttaaaaata acttgatctt
     3301 ctatcaatgc atgtgtgcca atgattccat tgagttctcc attttttaat tgttctaaaa
     3361 taatttttcg ttgtttcgtt ttggttgatc ctgttaatag ttcaattttt acatccatgc
     3421 cgtttaaaac ttcttggata ttttgaaaat gttgttgagc caaaatttca gtaggagcca
     3481 tcaatgccgc ttgataatta ttgtcaaatg ccatcaacat agaaaggagt gcaattattg
     3541 ttttaccaga acccacatca ccttgtaaca atcggttcat ttgcaaacct gataaggtat
     3601 cttttctaat ttcttttaat actctttttt gtgcattggt caaatcaaaa ggcagattat
     3661 tattgtaaaa gttattgaaa tatgtatcaa ttttttcaaa tagaatacct ttttcttttt
     3721 ttctaatcac tttatttttc aatagattga gttgaataaa gaataactct tcaaatttca
     3781 atcttttagt tgcattattt tggtcgtcaa tattttgagg aatatgtatt tgtttgtaag
     3841 cttcaaatct tgagattaaa tgataatgtt tcagcacata ttctggaata ttttcgtgta
     3901 tattttcagg ttttaattg
//
LOCUS       DAOQCW010000152         3907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_11670_length_3907_cov_5.075026, whole genome shotgun sequence.
ACCESSION   DAOQCW010000152 DAOQCW010000000
VERSION     DAOQCW010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11670_length_3907_cov_5.075026"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..699)
                     /locus_tag="PLS16_10650"
     CDS             complement(103..699)
                     /locus_tag="PLS16_10650"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNJ61585.1"
                     /translation="MEISNLIERSGAIRKFYNLPDLSIKDWAFLMSISSFRSIKKGDI
                     FIKAGTVSEELGFIIKGSFRFFYEKEDVEKTAYFVFENQIMASFEGILEKAPSKTTIQ
                     ALEDGEIFVIDYNKMRKMYHTSPKFENIGRLIGEYYILMLHNQLSSFLLDTPEERYLK
                     LVENNADLLNKVPLQYIASYIGVTPVSLSRIRKRIISK"
     gene            complement(740..1168)
                     /locus_tag="PLS16_10655"
     CDS             complement(740..1168)
                     /locus_tag="PLS16_10655"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNJ61586.1"
                     /translation="MLAEGIKAPIIKGVDFKGKNTILYFYPKDDTPGCTANACNFRDN
                     YQSLTKKGFTIIGVSPDNESSHAKFKEKYELPFELIPDTDKTIAKAYEVWGTKKMYGK
                     EYEGILRTTFVINNDGVITNIISKVDTKNATEQLLDTLGI"
     gene            complement(1192..2793)
                     /gene="pckA"
                     /locus_tag="PLS16_10660"
     CDS             complement(1192..2793)
                     /gene="pckA"
                     /locus_tag="PLS16_10660"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HNJ61587.1"
                     /translation="MDATISDKQTNNLEYLGVTNPNQTWNTTREDLINKSLELNLGTL
                     NDSGALCINTGEFTGRSPKDKFTVKDELTADSVDWNNINQPFAPEDFDKLQQRVVEYI
                     NTLDNIYIRDVSACADPKYKTNVRVITETPWASLFADNMFIRSSQEELKNFQPEWTIL
                     CIPSFHADKNIDKTRQHNFSIINFTKKVILIGGSSYTGEIKKGIFTILNYVLPHQKNV
                     LSMHCSANIGADGDTALFFGLSGTGKTTLSADPNRKLIGDDEHGWSDTGVFNFEGGCY
                     AKCIDLTEEKEPDIYRAIKPGAILENIGFHPETNQVNYEDTTITENTRVSYPIHHINN
                     IAEPSVGGIPKNIFFLSYDAFGVLPPISKLTPEQAMYLFISGYTSKVAGTEAGVTEPQ
                     TTFSACFGAAFLPLHPTEYADMLGKRLKESGANVWLINTGMTGGAYGVGQRMSLKYTR
                     AIIAEALNGHLENVAYETLPIFNLSIPTSCAGVPSEILNPRNTWTDKAAYDIKAKELA
                     IKFNENFKKYANLASEAILAAAPKA"
     gene            2970..>3907
                     /gene="bshA"
                     /locus_tag="PLS16_10665"
     CDS             2970..>3907
                     /gene="bshA"
                     /locus_tag="PLS16_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HNJ61588.1"
                     /translation="MNIGIVCYPTYGGSGVVATELGKALAKRGHKVHFITYREPARLL
                     DTFCANIYYHEVSLADYPLFEFAPYETALASKLVDVALHDDLDIFHVHYAIPHASAAY
                     MAKQILETKGKQIPVITTLHGTDITLVGKDPTYEPVVTYSINHSCGVTAVSEDLRRDT
                     LAHFNIEKDIKVIPNFIDFSRFKKTNKEHFRKAIAPNGEKILVHTSNFRKVKRIDDVV
                     KVFSKIQQIIPSKLLLIGDGPERQHIEILARNICKIDDVRFLGRQDAVEEILAISDLF
                     LMPSESESFGLAALEAMACEVPVISSDAGGIPEVNIT"
BASE COUNT         1278 a          605 c          590 g         1434 t
ORIGIN      
        1 ttttacaaag ataaagtctt atagataaat tgtttatgtt taacaagtct ttaactatgt
       61 aaagaatgac agtaatcata agtgaataaa taagataatt tattacttag aaataattct
      121 ctttcggatt ctgctcaaag aaactggtgt tacaccaata tatgaagcaa tatattgtaa
      181 aggaacttta tttaataaat ctgcattatt ttctactaat tttaaatagc gttcttctgg
      241 tgtatctaat aagaaagagg agagttggtt gtgaagcatt aaaatataat attctccaat
      301 taatcttcca atattttcaa attttggaga tgtatggtac atcttacgca ttttattata
      361 atcaataaca aatatttctc cgtcttctag tgcttgtatg gttgttttac taggtgcttt
      421 ctccaatatt ccttcaaaag atgccattat ctgattttca aaaacaaaat aggctgtttt
      481 ttctacatct tctttttcat aaaaaaatct aaaacttccc tttatgataa aacctaattc
      541 ttcagaaaca gtacctgctt taataaaaat atcacctttt tttatggaac gaaaactaga
      601 tatagacatt aagaatgccc aatcttttat ggataaatca ggtaaattat aaaattttct
      661 gattgctccc gaacgttcaa ttaaattgct tatttccatt cttcttattt tgaaattaca
      721 aaaaaaataa aaataattat tagataccta gagtatccaa taattgttcg gttgcatttt
      781 ttgtgtctac ttttgaaata atattagtta taacgccatc attatttatt acaaaagtag
      841 tacgtaatat accttcatat tcttttccgt acattttttt tgttccccaa acttcataag
      901 ctttcgctat tgttttgtcg gtgtctggaa ttaattcaaa tggtaattcg tatttttctt
      961 taaatttagc gtgagagctt tcattatcag gactaacacc aataattgta aatcctttct
     1021 ttgttaaaga ttgataatta tctctaaagt tgcaagcatt tgcagtacaa ccaggcgtgt
     1081 catcttttgg ataaaaatac aagatagtat tctttccttt aaaatcaact cctttaatta
     1141 ttggagcttt tatcccctct gctaacataa tgctatgttt taaagtttaa attatgcttt
     1201 tggagctgca gctaaaattg cttcacttgc taagttagca tattttttga aattctcatt
     1261 gaattttatt gccaactctt ttgcttttat atcataagct gctttatctg tccaggtatt
     1321 tcttgggttt aagatttcgc ttggtacacc agcacatgat gttggaatgc ttaaattaaa
     1381 gataggcaaa gtttcatacg caacattttc taaatgacca tttaaagcct ctgcaataat
     1441 agctctagta tatttcaaac tcattctttg tccaacacca taagcaccac cagtcatacc
     1501 agtgttgatt aaccaaacat tagctccact ttcttttaat cttttaccta acatatcagc
     1561 atattcagta ggatgtagag gcaagaatgc tgcaccaaaa caagcagaga aagtggtttg
     1621 tggctcagta acacctgctt cagttcctgc tactttagaa gtatagcctg aaatgaataa
     1681 atacatagct tgttcaggcg ttaattttga aattggaggc aatactccaa atgcatcata
     1741 acttaaaaag aaaatatttt ttggaatacc accaacagat ggttccgcaa tattattaat
     1801 atgatgaata ggatatgaaa ctctagtgtt ttcagttatt gtagtgtctt cgtaattaac
     1861 ttgattagtt tcaggatgga aacctatatt ttctaaaata gcacctggtt tgattgctct
     1921 ataaatatct ggttcttttt cttcagttaa gtcaatacat tttgcataac aaccaccttc
     1981 aaaattaaaa acacctgtat cactccagcc atgttcgtca tctccaatta gttttctgtt
     2041 tggatcagca gaaagtgttg tctttcctgt tcctgacaaa ccaaagaata atgcagtatc
     2101 accatcagct ccaatatttg ctgaacagtg catgcttaat acattttttt gatgaggtaa
     2161 tacataattc aaaattgtga atatgccttt ttttatctca ccagtatagc ttgaaccacc
     2221 aattaaaatt acttttttag taaagttgat gatagagaaa ttgtgttgtc tagttttgtc
     2281 gatattttta tctgcatgga aactaggaat acataaaatc gtccattcag gttggaagtt
     2341 ttttaattct tcttgagatg aacggataaa catattgtct gcaaataaag aagcccaagg
     2401 tgtttcagta attacacgaa catttgtttt gtattttgga tcagcacaag cacttacatc
     2461 tcttatatat atattatcta aagtgttgat atattctact actctttgtt gtaatttatc
     2521 aaaatcttct ggagcaaaag gttgattaat attattccaa tctactgaat ctgcagtgag
     2581 ttcatctttt acagtaaact tatctttagg cgatcttcca gtaaattcac cagtatttat
     2641 acataatgca cctgagtcat ttaaagttcc taaattaagt tctaatgatt tgttgattaa
     2701 atcttctctt gttgtattcc aagtctgatt tggatttgtt acaccaaggt actctaaatt
     2761 gtttgtttgt ttgtcactaa tagtagcatc cattttataa aagtattgat ttatgcaaaa
     2821 ataatcattt ttttatacat aaaaaagtaa gttcatttaa agttaatata agaggatatg
     2881 ataaatatca tttgtttatt cccactatat ttttactata attagtgtgc ttattattat
     2941 taattattga aaaaatgtaa cttgcatgta tgaatatagg aattgtttgc tatccaacct
     3001 acggtggaag tggtgttgtt gcaaccgaat taggcaaagc tttggcaaaa cgaggccaca
     3061 aagtgcattt tattacttat cgagagccag caagattatt agatacattt tgtgcaaata
     3121 tctattatca tgaagtaagt ttggcagatt atcctttgtt tgaatttgct ccttatgaaa
     3181 ctgcattggc aagcaaatta gtagatgttg cgttacatga cgatttagat atttttcatg
     3241 tgcattatgc gattccgcat gcatcagcag cttatatggc aaaacaaatt ttggaaacta
     3301 aaggaaaaca aattccagtc attacaactt tacatggaac agatattact ttagtaggga
     3361 aagacccaac ttatgagcca gtggttacct attcaattaa tcattcttgt ggagttactg
     3421 ccgtttctga agatttacgt cgtgatacct tagctcattt taatattgaa aaagatatta
     3481 aagtgatacc caattttatt gatttttcta gatttaaaaa aacgaataaa gaacatttta
     3541 gaaaagcaat tgcacccaat ggagaaaaaa ttttagtgca tacttctaat tttagaaaag
     3601 taaagcgtat agatgatgtt gtgaaagtat ttagtaaaat acaacaaatt ataccttcca
     3661 aattattatt aattggcgat ggcccagaaa gacaacacat cgaaatatta gcacgaaata
     3721 tttgtaaaat tgatgatgtt cgctttttag gaagacaaga tgctgtggag gaaattttgg
     3781 caatttcaga tttgttttta atgccctcag aaagtgagag ttttggtttg gctgcacttg
     3841 aagcaatggc ttgtgaagtt cctgttattt cttcagatgc aggtggtatt cctgaagtaa
     3901 atataac
//
LOCUS       DAOQCW010000153         3904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_11685_length_3904_cov_4.652117, whole genome shotgun sequence.
ACCESSION   DAOQCW010000153 DAOQCW010000000
VERSION     DAOQCW010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11685_length_3904_cov_4.652117"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1134)
                     /locus_tag="PLS16_10670"
     CDS             complement(64..1134)
                     /locus_tag="PLS16_10670"
                     /inference="COORDINATES: protein motif:HMM:NF025454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4268 domain-containing protein"
                     /protein_id="HNJ61589.1"
                     /translation="MTFSELKFREREHLQEWIANNPNSLGEELLIIQKEFSGFNETNE
                     RLDLLALDKLGNLVIIENKLDDSGKDVTWQVIKYASYCASLTKQDILKIYQDFLGSKA
                     DAQEMISEFFDNRDISEIVLNQGLNSQRLMLVAANFRKEVTSTVLWLMNFKIRLQCFK
                     VTPFAFGEQLFLNVEQILPTKDTEDFAISIATKAQEEIEVQESLKNRHLVRLKFWEQF
                     INASNQVNQLFANNSPSKESWIGKGIGMSGVNINLAMSNNYCRSEIIFNRGSQEENKE
                     LFDFIYQMKDKIESDFGGPLTWERMDDKITCRIKSQLDGVSYFEESDWKKMNEFLIDV
                     SVRMEKAFKEPIKKLNNYWKNK"
     gene            complement(1342..1608)
                     /locus_tag="PLS16_10675"
     CDS             complement(1342..1608)
                     /locus_tag="PLS16_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61590.1"
                     /translation="MYKNNINYISLSIDKNKDTLLLNYDNFFSNQFDSAHIMYDSTKI
                     KRYNFTGNRCTENRNIFCENNYVKSSVFTYTFTNQDYIEADTIR"
     gene            complement(1751..3538)
                     /locus_tag="PLS16_10680"
     CDS             complement(1751..3538)
                     /locus_tag="PLS16_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61591.1"
                     /translation="MKKHTIKFLTLLCITVMLYTSCKKDALQNQENNFEQKETKTSAG
                     ISIVLGAKQTNAYTVENMLEAYKNLKAKENLSRNSSASTSSLVLPDLATMQNILVANT
                     KYVRVLPRNDEDFDSLREVYDTLEFSDFPLDYDLSQVGNYYHDPSLPDSQITWQYVVL
                     KKSDNISSYFQQQLITDNIFLAENSPYYNQYPESFWDNLEAAALQRVGQSDKLVNPTS
                     PSSARYRPNGNIKVLDDQLAQIIGLEGAKIMAYASWRHSKAYTDINGNFSGNHTFITK
                     CSYFIKWESDNHRGRKYDIRSGWYGQAWTFLQGNSYNACYKTLDRSFQSGLPLFYASI
                     HRGIYDVYFHDPFGLSNPRIRLKIWGKDGGYLVNPNGTGIFHPIASIVGGPDIVIWRY
                     EQYLNGPIFEKNTLKTYGTTVHEVGHAIQRSLGIAQFAFCSNLIRESWADCVEWCFVM
                     HKYNAPNSIYASDTRFNVLNSPEVGANLNYIEYPSTNFIYNFDRNTSKVNLSYTPLFI
                     DIIDNVPCNSQFTISNFNNGISVCPKDVVGGYSLKEIENCLANKDWSVSWSANLDHIK
                     TWLYNNSTNPTKYSLAPYLQWYYNYYKGN"
     gene            complement(3576..>3904)
                     /locus_tag="PLS16_10685"
     CDS             complement(3576..>3904)
                     /locus_tag="PLS16_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61592.1"
                     /translation="SFHLSHADKIELKKISSPSEIKKYANAQPVTTKTEKRSIDNGLQ
                     GRTTRTTGYNSGFAKKRVQWLIEHSTSHQILWYVDSLVLRNPLLRKAAKRCKPFSEIC
                     ASNQIK"
BASE COUNT         1143 a          659 c          612 g         1490 t
ORIGIN      
        1 aggtagaatg ttcaattaac cacgtcaccc gccattgagc caaacgcctg ttagcacacg
       61 taattatttg tttttccaat agttattcag ttttttaatc ggttctttaa atgctttttc
      121 catccgaact gaaacgtcaa ttagaaattc gttcattttt ttccaatcgc tttcttcaaa
      181 ataactcaca ccgtcaagtt gggatttaat tctgcaagtt attttgtcgt ccatacgctc
      241 ccaagtcaaa ggcccgccaa aatcactttc tattttatct ttcatttggt agatgaagtc
      301 aaaaagttct ttattttctt cttggctacc acgattaaaa attatctcac ttctgcaata
      361 gttattactc attgcaaggt taatgtttac accactcatt ccgatgcctt tgcctatcca
      421 actttctttt gatggtgagt tatttgcaaa aagttgattt acttgattac ttgcgttgat
      481 gaattgttcc caaaatttca gtcttacaag atgtctgttt tttaaagatt cttggacttc
      541 aatttcttcc tgagcttttg tcgctatgct aattgcaaaa tcttctgtgt ctttggttgg
      601 tagtatttgt tctacattaa ggaaaagttg ttctccaaaa gcaaaaggtg tcactttaaa
      661 gcattgcaac cttattttga agttcattaa ccataaaacg gtagatgtaa cttcttttcg
      721 gaagtttgcg gctaccaaca ttaatctttg cgagtttaag ccttgattaa gcacaatttc
      781 agaaatatcc ctattgtcga aaaattccga aatcatttcc tgtgcgtcag ctttagagcc
      841 taaaaagtct tggtaaattt ttaagatgtc ctgctttgtc aaacttgcac aataggaagc
      901 gtatttaatt acttgccaag taacgtcttt gcccgagtcg tcaagtttgt tttcgatgat
      961 tacaagatta ccaagcttgt ctaatgcaag taagtcaagt ctttcatttg tctcgttaaa
     1021 tccgctaaac tctttttgaa tgataagaag ctcttctcca agtgagtttg ggttgttagc
     1081 aatccattcc tgtaaatgtt ctctttccct aaatttcagt tcggaaaaag tcatttgttt
     1141 cagtttggat attctatttt tttctttgtc aattagatac attctggtcg tcttttatta
     1201 tgtgtgctaa cggcgaagca ttgctgaagt gcggtatttt actaccacac tttttgcaat
     1261 gttctttctg taaagttatt taatttctgt tgttatttgt atttcacacc gcattttagc
     1321 aatgctatgt tgtaagccgt tttatctaat tgtatcggct tcaatataat cttggtttgt
     1381 aaaagtgtac gtgaaaactg atgattttac atagttattc tcacaaaata tgtttctatt
     1441 ctcagtacat ctattgcctg taaaattata tctcttgatt tttgtcgagt catacataat
     1501 atgtgctgaa tcaaattgat tcgagaagaa gttatcatag tttaataata atgtatcttt
     1561 gtttttatct atactaagcg agatataatt aatattattc ttatacattt ttatttttat
     1621 ttttgtgttt gttgaattaa tataattaat atcattaata gatatacatt tcccacaatc
     1681 tctatctgtg caggaacaca ttagagtact tattgttatg attaagatga ttgttttcat
     1741 tttatttatt ttaatttcct ttgtaataat tataatacca ttgtaagtat ggtgctaatg
     1801 aatactttgt agggttagtt gaattattat ataaccatgt tttaatatga tctaaatttg
     1861 cagaccaact aacagaccaa tctttattag ctagacagtt ttctatttcc tttaatgaat
     1921 atccaccaac aacatctttt ggacatactg aaattccgtt attaaaatta cttatggtaa
     1981 attgagaatt gcaaggaaca ttatcaataa tatcaataaa taatggtgta tatgataagt
     2041 ttactttcga tgtgttcctg tcaaaattat aaataaaatt tgttgatggg tattctatgt
     2101 agttcaaatt tgctcctact tcaggtgagt ttaacacatt aaatctcgtg tcacttgcat
     2161 aaatagaatt tggagcatta tacttatgca ttacaaaaca ccattctaca caatctgccc
     2221 aactttctct tataagatta gagcaaaatg caaattgtgc aatacctaat gaacgttgta
     2281 ttgcatgacc tacttcatgt acagtagtac catatgtttt caaagtattc ttttcaaata
     2341 taggaccatt taagtattgt tcatatctcc agattacaat atctggacct cctacgattg
     2401 acgcaattgg atgaaaaata ccagtaccat ttgggtttac caaataacca ccgtctttac
     2461 cccagatttt caatctaatt cttggattgc ttaagccaaa tggatcgtga aaatacacat
     2521 cataaattcc tctgtgaata gatgcataaa atagtggcaa tcctgattga aaactcctat
     2581 ctaatgtttt ataacaagca ttatatgaat tgccttgtaa aaatgtccat gcttgaccat
     2641 accaaccgct tctaatatca tattttctac ctctatggtt atcgctttcc cattttatga
     2701 aataagaaca tttagttata aatgtgtgat tgccactaaa attgccgttt atatctgtgt
     2761 atgctttact atgtcgccaa cttgcataag ccattatctt tgcaccttct agtccaataa
     2821 tttgagccaa ttggtcatca agtactttta tatttccatt gggtctatat ctagcggagc
     2881 ttggtgatgt cggatttact aacttatcag attgtccaac acgttgtaga gcagctgcct
     2941 ctaaattatc ccaaaacgat tcaggatatt ggttatagta tggagaattt tctgcaagaa
     3001 atatattgtc agttataagt tgttgctgaa aatatgatga aatattatct gatttcttta
     3061 atacaacata ttgccatgtt atttgtgaat caggcagaga tgggtcgtga taataatttc
     3121 ctacttgcga taaatcataa tctaacggaa agtcagaaaa ttctaaagta tcataaactt
     3181 cgcgtaatga atcaaaatct tcgtcatttc gtggtaaaac ccgaacatac ttagtatttg
     3241 caactaaaat attttgcata gtagctaagt caggaagtac caatgaactt gttgaagcag
     3301 atgaatttct acttagattt tcttttgctt tcaaattttt gtatgcttct aacatatttt
     3361 ctacagtata agcatttgtt tgttttgctc ctaatacaat cgaaatacca gcactagttt
     3421 tggtttcttt ttgttcaaag ttgttttctt ggttttgtaa tgcatctttc ttacaacttg
     3481 tgtagagcat gacagtaata cataataaag ttaagaattt aatagtgtgt tttttcatac
     3541 cgttgttttt ttattttaag ttaacgaata gattttcatt ttatttgatt gcttgcacaa
     3601 atttcagaaa atggcttaca acgttttgcg gctttgcgaa gaagcggatt tcggagcact
     3661 aaactgtcaa cataccacaa aatttgatgc gaggtagaat gctcaattaa ccactgaacc
     3721 cgcttttttg caaaaccgct gttatagcca gtggttcttg tcgttcgtcc ttgcaaacca
     3781 ttgtcaatac tacgtttctc tgtctttgtg gtgacgggtt gtgcgtttgc gtatttttta
     3841 atttctgatg gggaggagat tttttttaat tcaattttgt ctgcgtggga aaggtggaag
     3901 ctct
//
LOCUS       DAOQCW010000154         3889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_11771_length_3889_cov_5.506781, whole genome shotgun sequence.
ACCESSION   DAOQCW010000154 DAOQCW010000000
VERSION     DAOQCW010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11771_length_3889_cov_5.506781"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PLS16_10690"
     CDS             <1..196
                     /locus_tag="PLS16_10690"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNJ61593.1"
                     /translation="FDFLSGLYIRAPKLIRTLKLEWFWRTMLHPTRHYQKRLRDTTII
                     FRPFLDRWANVKPYFNIINK"
     gene            313..1518
                     /locus_tag="PLS16_10695"
     CDS             313..1518
                     /locus_tag="PLS16_10695"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006090.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNJ61594.1"
                     /translation="MTEAYIYDCVRTPRGKGKKNGALHEVTPTELLAQMLKAIQKRNN
                     LDTSLVEDVIVGCVTPIGDQGGNIAKTAIMMADYPDSVPGFQLNRFCSSGLEAVNLAA
                     AKIRSGWSDLIIAGGVESMSRVPMGYDGGAWVLDMRVNDHTNFVPQGVSADLIATIEG
                     FSRTDADNYALRSQKLAAIAKAEGRFKKSIVPIVDINGLPILEEDEYNRPDTTIEGLS
                     SLAPSFQMMGEMGFDAVALQKYTQLEQINHIHTPGNSSGIVDGASLILVGSKAIGEQL
                     GLKPRARIVSIGTTSTEPTIMLTGPGPASLIALKKAGMTINDIDLVEMNEAFASAVLR
                     LQRDINVPDEKLNVNGGAIALGHPLGATGAMILGTIVDELERTNKATGLATLCVGGGM
                     GVTTIVELV"
     gene            1696..3501
                     /locus_tag="PLS16_10700"
     CDS             1696..3501
                     /locus_tag="PLS16_10700"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNJ61595.1"
                     /translation="MATKQSNLVGGSFLLKDAQLDDIFIPEQFSEEQNGIKDMVIDFC
                     KQEIHSMGFEKLSALSADKDLDKVKEIFDKASELGFCAGSIPETYGGMGLDFNTGLVF
                     SEAIALGFSFATTIGAQTSIGSLPIVWYGTEAQKQQYLPKIASGEYACSYCLTEPTSG
                     SDANSAKTNAVLNAEGTHYLINGQKMWITNGGFADVFIVFAKIENDERLSAFIVEKEF
                     GGIEIGKEEKKMGIKASSTVQLFFNNCPVPKENLLGERGKGFNMALNILNSGRIKIAA
                     GATGGIKFGTKTSVEYAAQRIQFGKPISDFGAMKQKIGNMVMQGFVLESAVYRTGADV
                     DKKYDELKAQGKSENDCKIDAMREYAIECAILKCQGSEAMCNAADEAIQIFGGMGYSM
                     ETGVEMAYRDARITKIYEGTNEINRMLSLAEFYKRAFQTKEIKLKDASTKIPLGLAQN
                     LNPFSNGKFDKEEEIVNNLKNLFLIITGAAGRKLKTKLVDEQEIVMNLADILAMAYMT
                     ESALLRVKKLASMKDVDAKDLEMKTKAMQLYIYEALDVARKSAANAIDSYASGVEKFA
                     MRRLVDGLLRAYEINPKDMRRALADYAVEKMDYPF"
BASE COUNT         1371 a          486 c          747 g         1285 t
ORIGIN      
        1 ttttgatttt ttgagtggtt tgtatatacg tgctccaaaa ttaataagaa ctttaaagtt
       61 ggaatggttt tggagaacga tgttacatcc aacaagacac tatcaaaaaa gattaagaga
      121 tactactatt atttttagac catttttaga tagatgggct aatgttaagc catattttaa
      181 tattatcaat aaataaaaga taagaatttt acttttttac taaattttta gatgcaaaaa
      241 gatgcattat tgttttttct ttctatattg gttaaaataa attttaaact aaattaaact
      301 aaacaaacga atatgactga agcatatatc tatgattgcg ttagaacgcc aagaggaaaa
      361 ggtaaaaaga atggtgcatt gcacgaagtt acaccaacag aacttttagc acaaatgtta
      421 aaagcaatcc aaaaaagaaa taatttagat acttctcttg tggaagatgt tattgtcggt
      481 tgcgttacac caataggtga tcaaggtggt aatattgcaa aaacggccat aatgatggca
      541 gactatccag attcagtacc tggttttcaa ttgaatcgct tttgttcatc tggattagaa
      601 gcggtaaatt tagcagctgc aaaaattcgt tctggttgga gtgatttaat aattgctggt
      661 ggtgttgaat ctatgagtcg tgtgccaatg ggttatgatg gtggtgcttg ggttttagat
      721 atgcgtgtta atgatcatac taattttgta ccacaaggcg taagtgcgga tttaattgca
      781 actattgaag gtttttctag aacagatgcg gataattatg ctttacgttc tcaaaaatta
      841 gctgcaattg ctaaagcaga aggtcgtttc aaaaaatcta tagttccaat tgtagatatt
      901 aatggcttac caattttgga agaagatgaa tacaatcgac ctgatactac tatcgaagga
      961 ttgagttcat tagctccatc ttttcagatg atgggcgaaa tgggttttga tgctgttgct
     1021 ttacaaaaat atacacaatt agagcaaatt aatcatattc atacaccagg aaattcttca
     1081 ggaattgttg atggagctag tttaatttta gttggttcta aagctattgg tgagcaatta
     1141 ggcttaaaac caagagcaag aattgttagt atcggaacaa cttctactga gcctacaata
     1201 atgctcacag gacctggccc agcatcttta atagccttaa aaaaagctgg aatgactatt
     1261 aatgatattg atttagtaga aatgaatgaa gcttttgcct ctgcggtttt acgcttacaa
     1321 agagatataa atgtacctga tgaaaaatta aatgtaaatg gtggtgcaat tgcattaggt
     1381 catcctcttg gtgcaactgg tgctatgatt ttaggtacta ttgttgatga attagagcga
     1441 actaataaag ctactggctt ggcaactctt tgtgttggtg gaggaatggg tgttacaaca
     1501 attgtagaat tagtataacc tagaatatat aagaaatgat aaaagagtct cttttgtgag
     1561 actctttttt attgtcaaaa aaagcttgtt tttattagta ttacacctaa acaatcattt
     1621 aaagtttaat taatctaaaa ttcaatgcat gcattgaatt tatagataac tttattcaaa
     1681 attttaatca atattatggc aacaaagcaa tctaatttag taggaggctc ttttttattg
     1741 aaagatgcac aattggatga tatatttatc ccagaacaat tttcagaaga acagaatgga
     1801 attaaagaca tggtaattga tttctgtaaa caagaaattc atagcatggg ttttgaaaaa
     1861 ttatcagcat taagtgccga taaagatttg gataaagtaa aagaaatttt tgataaagca
     1921 tctgaacttg gtttttgtgc tggttcaatt cctgaaactt atggtggaat gggattagat
     1981 tttaatactg gtttggtatt tagtgaagca attgcattag gattttcttt tgcaacaact
     2041 attggagctc aaacatctat tggttcatta ccgattgttt ggtatggtac agaagctcaa
     2101 aagcaacaat acttacctaa aattgcaagt ggtgaatatg cttgttcata ttgtttaact
     2161 gaaccaactt ctggttctga tgcaaatagt gccaaaacaa atgctgtttt gaatgcagaa
     2221 ggtacgcact atttaatcaa tggacaaaaa atgtggatta ccaatggtgg ttttgcagat
     2281 gtatttattg ttttcgctaa aattgaaaat gatgaaagat tatcagcatt catcgttgaa
     2341 aaagaatttg gtggaataga aattggaaaa gaagaaaaga aaatgggtat taaagcatct
     2401 tctacagttc aattattctt caataactgt ccagttccaa aagaaaactt attaggagaa
     2461 agaggaaaag gatttaatat ggctttgaat atcttaaata gtggtagaat aaaaatcgct
     2521 gctggagcaa ctggcggtat taaatttggt actaaaactt ctgttgaata tgcagctcaa
     2581 agaattcaat ttggtaagcc aatttctgat tttggtgcaa tgaaacaaaa aatcggaaat
     2641 atggttatgc aaggatttgt tttagaatca gcagtttaca gaactggtgc tgatgttgat
     2701 aaaaaatatg atgaattaaa agcacaagga aaatctgaaa acgattgtaa aattgatgca
     2761 atgcgtgagt atgctatcga atgtgccatc ttaaaatgcc aaggttctga agcaatgtgt
     2821 aatgctgctg atgaagcaat tcaaattttt ggaggtatgg gttattctat ggaaactggt
     2881 gttgaaatgg cttatagaga tgctcgtatt actaaaattt atgaaggaac aaatgaaatc
     2941 aacagaatgt tatcgttggc tgagttctat aagagagcat tccaaactaa agaaattaaa
     3001 ttaaaagatg catctacaaa aattccttta ggtttagcac aaaacttaaa tcctttttct
     3061 aatggtaaat ttgataaaga agaagaaatc gtaaataatt tgaaaaactt attcttaatt
     3121 atcacaggag ctgctggtag aaaattaaaa acaaaattgg ttgatgagca agaaatcgtt
     3181 atgaatttag cagatatctt ggctatggct tatatgacag aatcagcttt attgagagtt
     3241 aaaaaattag cttcaatgaa agatgttgat gctaaagatt tagaaatgaa aactaaagca
     3301 atgcaattat atatctacga agcattagat gttgcaagaa aatctgcagc aaatgctatt
     3361 gatagttatg caagtggcgt agaaaaattt gccatgagaa gattggtaga tggtttatta
     3421 cgtgcttatg aaatcaatcc aaaagatatg agaagagcat tggcagatta tgcagttgag
     3481 aaaatggatt atccattcta atgtatagat tgaattttaa aagagaggct gtccgaaaag
     3541 gcagcctctt tttttttagt ttttttctgg attagtgatt aattaaataa tgaagtaatg
     3601 aagtaaagta ataatgaatt tctcaatact cggcaattgt ctgaatcagg atttatagga
     3661 ttttaggatg aacaggattt ttttagtggt tagtgatgta atgtaatagt gaagtaatgt
     3721 aataatggga gtaattaatt tctaactact caatactttc tacttatatc tcaataaagt
     3781 cttgactctt gactcttgac tctaatctct cactacttaa tactcatact gtgttactta
     3841 gtcaaataag aatgcgatat cttcttggct taaatttttt acgaagcta
//
LOCUS       DAOQCW010000155         3636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_13296_length_3636_cov_4.489528, whole genome shotgun sequence.
ACCESSION   DAOQCW010000155 DAOQCW010000000
VERSION     DAOQCW010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13296_length_3636_cov_4.489528"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PLS16_10705"
     CDS             <1..447
                     /locus_tag="PLS16_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNJ61596.1"
                     /translation="ASLADYEQLVEYTHAIYQRGTDLAKEHHLILVDTKYEFGKIGNQ
                     IFLMDEIHTPDSSRYFYADGYEERLEKGEPQKQLSKEFVREWLIANDFMGKEGQTVPQ
                     MTPEVIENISKRYIELYEHILGKTFEPIDAPQNAEEIIIQCIERYS"
     gene            616..2991
                     /locus_tag="PLS16_10710"
     CDS             616..2991
                     /locus_tag="PLS16_10710"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNJ61597.1"
                     /translation="MSVLNNITGVLLKPIIKEMSRAALPKIDGNIYVDGLSNEVEIIR
                     DEWGIAHIYTDKLEDALFAQGYIHAQDRLWQMDLNRRAARGKLAEIIGKDALDADRIA
                     RTFGYDRIGKKDWELLTDDLKSYLKAYCNGVNAFIENQTSRFPVEYKLLGLKPELWNP
                     IDVCAFSKMLTSLLTFGWYDEIIRAKLIELLGLEAVLELDNSYPENNPVTLPKGVEFN
                     TIDIDNKFNAMNGPYIPKISGSNAWTIHGSKTKTGKPYLCNDPHLTLKNPNIWYQMHI
                     HTKDYHVSGVSCPAIPAILIGHNDNIGWGITLSFTDIEDVYVEKFTDETMKTYMYDGK
                     IMDSEIIEEKIYIKGENMPHIEKVISTVNGTLISHVTNHKAQAYTLCSMAFRPGKMFE
                     GWFNLNKARDWNDFVKAVECIQAPGLNIVYADIVGNIGYYNSGKVPIRDKQIASVPMP
                     GWTSENKWSDFVPFEKMPHVLNPQQGHVITANHKIVADDYPYFLGDIYMNGYRANRLE
                     KMIEGKTNLSPKDFTEMQMDWTCLPAEQFKDYYTNIDMGNTELQQYVQMLLNWDGVLR
                     PEQIEGSLYKVCKKEMVKYIYDRNIEDKNLVNELLGAGFHEIFQPLNAFLGHNTVALF
                     RILDNPDSILLKNAGGKEHVLKTGIKYGIDWLKLNFGTNPKKWNWGSVHAIVFPHAFS
                     VKPPLDKVFNVGPFPIGGDTDTPFQTYVMAENAYGGELNSVSYRQTIDFADFDNSTII
                     MPLGNSANMASPFYKNQLDKWFKGESIAMCFSRKKVEEHKKHTLWLKKKTK"
     gene            complement(3011..>3636)
                     /locus_tag="PLS16_10715"
     CDS             complement(3011..>3636)
                     /locus_tag="PLS16_10715"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNJ61598.1"
                     /translation="PIVYGMTIGEYAQMLNGEKWLTNKAQCKLTVIKNQNYDHNTFYS
                     LPIKPSPNLPNIQSIYLYPSLCFFEGTNTVSLGRGTDFPFQIYGSPDFPNTLGYQFTP
                     IPKEGAKNPPLLNQKCYGYNLSETNLDTLKQQKFTLKYLINAYQLSSAKPTFFTSFFE
                     KLAGTSTLRKQIESGMNENAIKATWQKDLNCFKLIRKKYLLYPDFKE"
BASE COUNT         1300 a          475 c          667 g         1194 t
ORIGIN      
        1 gcatcgttag cggattatga gcaattggta gaatacacac acgcaattta tcaacgaggt
       61 acagatttgg caaaggaaca tcacttaatt ttagtggata caaaatatga atttgggaaa
      121 attgggaatc aaatattttt gatggatgaa attcatacac ctgattcttc aagatatttt
      181 tatgctgatg gctatgaaga aagattagaa aaaggagagc cacaaaaaca gttgtcaaag
      241 gaatttgtac gcgaatggtt gattgctaat gattttatgg gtaaagaagg acaaactgtt
      301 ccacagatga caccagaagt tattgaaaat atttctaaaa gatatattga gttatacgaa
      361 catattttag gtaaaacttt tgaaccaatc gacgcacctc aaaatgcaga ggaaattatt
      421 attcaatgta ttgaaaggta ttcgtaattg atgttgtttt aaaagcctat tatttattta
      481 aaaagaaagc tattttgttt gaagcgaacg gctaaaacgt aaaatttgag tagaacaata
      541 aaagtatcat tgcacactaa aatctaataa tttctataaa ttagtttatc tttagtatcc
      601 aaataagata attgtatgtc tgtacttaat aatattacag gagttttatt aaaacccatc
      661 atcaaagaaa tgagtcgagc ggcattacct aaaattgacg gaaatattta tgtagatggc
      721 ttgagtaatg aagtagaaat tatacgtgat gaatggggaa ttgcacatat ttatacagat
      781 aaattagaag atgcactttt tgctcaaggt tatattcatg cacaagacag attgtggcaa
      841 atggatttga atcggcgtgc ggctcgtggt aaattagcag aaattattgg gaaagatgcc
      901 ttagatgccg accgtatagc tcgaactttt ggttatgata gaataggaaa gaaggattgg
      961 gaattattga ctgatgattt aaaatcttat ctgaaggcat attgtaatgg tgttaatgct
     1021 tttattgaaa atcagacttc tagatttcca gtggaatata aattattagg tctaaagcca
     1081 gaattatgga atcctattga tgtttgtgcc ttttctaaaa tgctgacttc tttactaact
     1141 tttggatggt atgatgaaat tattagagca aagctgatag agcttttagg tctcgaagct
     1201 gtcttagaat tagataattc ctatccagag aataatcctg taactttacc taaaggtgtt
     1261 gaatttaata caattgatat tgataataaa ttcaatgcaa tgaatggtcc atatatacca
     1321 aaaataagtg gaagtaatgc ttggacaatt catggaagta aaacgaaaac aggtaagcca
     1381 tatttatgta atgacccaca tctaacactt aaaaatccga atatttggta tcagatgcat
     1441 attcacacca aagactacca tgtttcaggc gtttcctgtc ctgcaattcc agcgatattg
     1501 attggacata atgataatat aggttgggga attactttat cttttacaga tattgaagac
     1561 gtttatgtag agaaatttac agacgaaacg atgaaaacgt atatgtatga tggaaaaatc
     1621 atggactctg aaattataga agaaaaaatc tatatcaaag gggaaaatat gccacatatt
     1681 gaaaaagtaa tcagtacggt aaatggtact ttaatttctc atgttacaaa ccataaagct
     1741 caagcatata cattatgttc gatggcattt cgaccaggaa aaatgtttga aggttggttt
     1801 aatctaaaca aagctagaga ttggaacgat tttgtaaaag ctgtcgaatg tattcaagca
     1861 cctggattaa atattgttta tgcagatatt gtaggaaata tcggttatta taattctgga
     1921 aaagtaccta ttcgagataa gcaaattgct tctgtgccta tgccaggttg gactagcgaa
     1981 aataaatggt cagattttgt tccatttgaa aagatgccac acgttctaaa tccacaacaa
     2041 ggtcatgtaa ttactgcgaa ccacaaaata gtagcagatg attatcctta ttttcttggt
     2101 gatatttata tgaatggtta tcgtgcgaat cgtttggaaa aaatgattga aggaaaaact
     2161 aatttaagtc ctaaagattt tactgaaatg caaatggatt ggacttgctt gccagcagaa
     2221 caatttaaag attactatac caatattgat atgggaaata cagaacttca acaatatgta
     2281 caaatgcttc taaattggga tggtgtattg cgaccagaac aaattgaagg ttctttgtat
     2341 aaagtctgta aaaaggaaat ggtaaaatat atttacgata gaaatattga agataaaaat
     2401 ttagtaaatg aattattggg tgctggattt catgagattt ttcaaccttt aaatgcattt
     2461 cttggacaca ataccgttgc actttttaga attttagaca atccagattc tatattgtta
     2521 aagaatgctg gaggcaaaga acatgtgcta aaaacaggta tcaaatatgg aatagattgg
     2581 ttgaaactaa attttggtac aaatcctaaa aaatggaatt ggggcagtgt tcatgcaatt
     2641 gtatttccgc atgcattttc agtaaaacca ccattagata aagtatttaa tgttggtcca
     2701 tttcctattg gtggagacac cgatacgcca tttcaaactt acgtaatggc agaaaatgct
     2761 tatggaggag aattaaattc agtttcatat aggcaaacta tcgactttgc agattttgat
     2821 aattctacga ttataatgcc actcggaaat agtgccaaca tggcgagccc attttataaa
     2881 aatcaattag ataaatggtt taaaggcgaa agtattgcga tgtgtttttc tagaaaaaaa
     2941 gtggaggaac acaaaaaaca tacactttgg ctaaagaaaa aaacaaaata ataaatgttg
     3001 ttttttttaa ttattcttta aaatcaggat aaagtagata ctttttgcga attaatttaa
     3061 aacaattcaa atctttttgc caagtagctt ttatagcatt ttcattcatt ccactttcaa
     3121 tttgttttcg taaggttgag gttcctgcta gtttttcaaa aaatgatgta aagaaagtag
     3181 gtttggcact acttaattga taagcattaa taaggtattt taaagtaaat ttttgttgtt
     3241 ttaaagtatc taaatttgtt tctgataaat tatatccata gcatttttga tttagtaaag
     3301 gtggattttt ggcaccttct tttggtattg gtgtaaattg ataacctagt gtattcggaa
     3361 aatctggact tccataaatt tgaaatggaa aatcagttcc tctacccaaa cttacagtat
     3421 ttgtgccttc aaaaaaacat aaacttgggt acaaataaat actttgtata tttggtaagt
     3481 taggagatgg ttttataggt aaactataaa aagtattgtg gtcgtagttt tgatttttaa
     3541 taacagttaa tttgcattga gctttattag ttagccactt ttctccattc aacatttgag
     3601 catattctcc tatcgtcatt ccgtaaacaa ttggaa
//
LOCUS       DAOQCW010000156         3536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_13993_length_3536_cov_5.414536, whole genome shotgun sequence.
ACCESSION   DAOQCW010000156 DAOQCW010000000
VERSION     DAOQCW010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13993_length_3536_cov_5.414536"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="PLS16_10720"
     CDS             <1..264
                     /locus_tag="PLS16_10720"
                     /inference="COORDINATES: protein motif:HMM:NF036577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor"
                     /protein_id="HNJ61599.1"
                     /translation="EKYTLEVSSPGLDQPFKVPQQFEKSINQKVEVLKVDGEKFIGTL
                     LVYDGEKVTIKSEKKIKKEIKEEIIEINLQEIKSIKKHFIFKV"
     gene            270..1505
                     /gene="nusA"
                     /locus_tag="PLS16_10725"
     CDS             270..1505
                     /gene="nusA"
                     /locus_tag="PLS16_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNJ61600.1"
                     /translation="MNTADLIESFSEFKDFKNINRPTMMRVLEDVFKTLLRKKYTSDQ
                     NFDIIVNPDKGDLEIWRHRDVVEDGAVEDRLAQVEISYAKKIEPDMEVGEELYEPILL
                     ESFGRRSVLAARQTLISRILDLEKDEVYKKYKDRIGEIISAEVYQVWKKEILLLDDDG
                     NELILPKSEQIKTDFYKKGDTIRAVIKDVELRGNTPQVILSRTAPEFLAKLLEQEVPE
                     IFDGLIIIKKIVREPGERAKVTVESYDDRIDPVGACVGMKGSRIHGIVRELRNENIDI
                     INYTNNLMLLIQRALTPAKIESISVDEEKKKVSVFLKPDQVSLAIGKGGYNIKLAGKL
                     TGYEIDVFRDTDEIQDDVDLEEFKDEIDDWIIKILKDMGCDTAKNVLEIDRDDLIRRT
                     DLEEETVDEIIRILSAEFE"
     gene            1545..>3536
                     /gene="infB"
                     /locus_tag="PLS16_10730"
     CDS             1545..>3536
                     /gene="infB"
                     /locus_tag="PLS16_10730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNJ61601.1"
                     /translation="MSETKTIRLASAAKELNVGIGTIVDHLQSKGYSEIDSKPNTKLT
                     EEQYNVLLKDFSSSIEIKERAEQLNIGKRKDEEEIYVKKEEKVEKVEVPEPKEEPKPE
                     PEIKEVKIEPISTESEIDTLKVVGKIDLDALNKKPAKKTIKKKEEVATETTTKTKVEL
                     ESKKDEIQPEAPKEKAEVVEKVVAIEPEPEKEVELIRAEAEKLAGPKILGKIELKQEK
                     RPEQIYRQEEKQRKEKEEEKKKEEAREQEKKKRARIHANKVDVKVFEKSNPGKTSDNN
                     FRKDNNFRKGGKFTKPEPEAVSEKDINDKIKATMAKLAGTRGKSTGVKYRRDKREEHA
                     RRAEEESVDSKVLQVTEFITVSELASLMNVEPTAIIKVCMGLGVFVSINQRIDAEIIE
                     LVAGEFDYEVQFTTITEQEEEEMAEVDDPKDLVPRSPIVTIMGHVDHGKTSLLDYIRD
                     GNVVAGEMGGITQHIGAYEVTTAKGNKITFLDTPGHEAFTAMRARGAKLTDIVIIVVA
                     ADDKVMPQTREAISHAQAAGVPMIFAINKIDKPAANPDAVRTQLSEMNILVEEWGGKY
                     QCQEISAKKGLNIDILLEKILLEAELLDLKANPNKKAIGSVIEASLDKGRGYVSTVMV
                     QDGTLKIGDVMVAGQYHGKVKALYNERGLRINSAGPSSPV"
BASE COUNT         1456 a          427 c          709 g          944 t
ORIGIN      
        1 gaaaaatata ctttagaagt atcatcaccc ggtttagatc aaccatttaa agtgccacaa
       61 caatttgaaa aatctatcaa tcagaaagtt gaagttttaa aagtagatgg cgaaaaattt
      121 attggaacac ttttggttta tgatggcgaa aaggttacga taaaatctga aaaaaaaatt
      181 aagaaagaaa taaaagaaga aattatagaa ataaatcttc aagaaatcaa atcaattaaa
      241 aaacatttta tttttaaagt ataataagta tgaatactgc agatttaata gagtcatttt
      301 ccgagtttaa ggactttaag aacattaaca gacccacaat gatgagggta ttagaagatg
      361 tatttaaaac attacttaga aagaaatata catctgacca aaattttgat attatcgtaa
      421 atcctgataa gggcgactta gaaatatggc gacatcgtga tgttgttgaa gatggtgcag
      481 ttgaagatag attggcacaa gtagagataa gttatgctaa gaaaattgag ccagatatgg
      541 aagtgggcga agaattatat gaaccaattt tattagaatc atttggtcgt cgttcagtat
      601 tggctgcaag acaaacttta atttcaagaa ttttagattt agaaaaagac gaagtttata
      661 aaaaatataa agatagaatt ggtgaaatta tcagtgctga agtttatcaa gtttggaaaa
      721 aagaaatttt gttattagat gatgatggta atgaattaat cttacctaaa agcgaacaaa
      781 ttaaaactga tttctataaa aaaggcgata cgattcgtgc cgttattaaa gatgtagaat
      841 taagaggtaa tacacctcaa gttattttgt ccagaactgc accagaattt ttagctaaat
      901 tattagaaca agaagtgcct gaaatttttg atggcttaat cattatcaaa aaaatagtaa
      961 gagaaccagg tgaacgtgca aaagttactg ttgaatctta cgatgataga attgacccag
     1021 taggtgcatg cgttggtatg aaaggttctc gtattcacgg tattgtaaga gaattaagaa
     1081 acgaaaatat cgatatcatc aattatacaa ataatttgat gttgttaatt caacgtgcat
     1141 tgacaccagc aaaaattgaa tctatttcag ttgatgaaga aaagaaaaaa gtttctgtat
     1201 ttttgaaacc agatcaagta tctttggcaa ttggaaaagg tggatacaat attaaattgg
     1261 ctggaaaatt aactggttat gaaatagatg tatttagaga tacagatgaa atccaagatg
     1321 atgttgattt agaagaattt aaagatgaga ttgacgattg gattatcaaa atcttaaaag
     1381 atatgggctg tgatacggct aaaaacgtat tggaaattga tagagacgac ctaattagac
     1441 gaacagattt agaagaagaa actgttgatg agattataag aattctgagt gcagaatttg
     1501 aataattatt actttaaaac aaaaaattat ttttataaca taatatgtcc gaaacaaaaa
     1561 caataagatt agccagtgct gccaaagaat taaatgttgg cataggtact attgtagatc
     1621 acctacaaag caaaggctat tctgaaatag acagcaaacc aaatactaaa ctaacagagg
     1681 aacaatacaa tgtacttcta aaggatttct cttcatcaat agaaattaaa gaaagagccg
     1741 aacaattaaa tatcggtaaa cgtaaagatg aagaagaaat ttatgtaaaa aaagaagaaa
     1801 aagttgagaa ggtagaagtt cctgagccaa aagaagagcc aaagccagaa cctgaaatta
     1861 aggaagtgaa aatagaacca atttctactg aatcggaaat tgatacactt aaagttgttg
     1921 gtaaaattga tttagatgca ctaaataaaa aaccagcaaa gaaaactatt aagaagaaag
     1981 aagaagttgc tactgaaact acaacaaaaa ctaaagttga attagaatca aagaaagatg
     2041 aaattcagcc agaagcccca aaagagaaag ctgaagttgt tgagaaggtt gtagcaatag
     2101 aaccagaacc tgaaaaagaa gtagaattaa taagagctga agcagaaaaa ttagcaggtc
     2161 ctaaaatctt aggaaaaatt gaattaaagc aagaaaaaag accagagcaa atttacagac
     2221 aggaagagaa gcaacgtaaa gaaaaggaag aggaaaagaa aaaagaggaa gccagagaac
     2281 aagaaaaaaa gaaacgtgct agaatacatg ccaataaagt ggatgtaaaa gtatttgaaa
     2341 aatcaaatcc aggaaaaaca tcagataata atttcagaaa agataataat ttcagaaaag
     2401 gaggtaagtt tacaaaacct gaaccagaag cggtttctga aaaggatatt aacgacaaga
     2461 ttaaagccac aatggctaaa cttgctggaa ctagaggaaa atcaactggt gtaaaataca
     2521 gaagagataa acgtgaggaa catgcacgaa gagctgaaga agaatcagta gattcaaaag
     2581 tattacaagt tacagagttt attactgttt ctgaattagc aagtttaatg aatgttgaac
     2641 caacagcgat tattaaagtt tgtatgggat tgggtgtctt cgtatctatt aatcaaagaa
     2701 ttgatgctga aattatagag ttggtggctg gtgagtttga ttatgaagta caatttacta
     2761 cgattacgga gcaagaagaa gaggaaatgg ctgaagttga tgacccaaaa gatttagtac
     2821 caagatcacc aattgttacg attatgggac acgtggatca tggtaaaaca tcgttattgg
     2881 actatatccg tgatgggaat gtagttgctg gtgagatggg tggaattaca caacatattg
     2941 gagcttatga agtaacaact gcaaaaggta ataaaattac tttcttagat acaccaggtc
     3001 acgaagcatt tacagcgatg agagctaggg gtgcaaaatt gacagatatt gtaattatcg
     3061 tagttgctgc cgatgataaa gttatgccac aaactcgtga agcaattagt cacgcacaag
     3121 cagctggtgt accaatgatt tttgctatta ataaaattga taaacctgca gctaatccag
     3181 atgctgttcg tacgcaactt tctgaaatga atattttagt agaagaatgg ggcggtaaat
     3241 atcaatgtca agaaatttct gccaaaaaag gtttaaatat tgatattctt ttagagaaaa
     3301 tattattaga agcagaatta ttagacctaa aagcaaatcc aaataaaaaa gcaattggct
     3361 ctgtaattga ggcaagttta gataaaggac gtggatatgt ttcaacagtg atggtacaag
     3421 atggaacatt aaagattggt gatgtgatgg ttgctggtca atatcatgga aaagtaaaag
     3481 cattatacaa tgagcgtgga ttgcgaatta attcagcagg accatcttcg ccagtt
//
LOCUS       DAOQCW010000157         3487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_14362_length_3487_cov_5.171911, whole genome shotgun sequence.
ACCESSION   DAOQCW010000157 DAOQCW010000000
VERSION     DAOQCW010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14362_length_3487_cov_5.171911"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="PLS16_10735"
     CDS             <1..550
                     /locus_tag="PLS16_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61602.1"
                     /translation="LDANHLNYKELAGEDFDMWIQIAKHSKIYNIPKPMLRYRIHQGN
                     MIHSNWYKLDDGLFSIIKEQLFYYFQSKITNDDVEAHYSLINFNRMNQLDELPNVYEW
                     LKKIINLNNEFKVYNPIALKKVLSERMTKKLMRMSANSFMLTIYLIKCFNLLGFKNIF
                     FNINRSIYIVFLSMFRIKIKNV"
     gene            543..1316
                     /locus_tag="PLS16_10740"
     CDS             543..1316
                     /locus_tag="PLS16_10740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HNJ61603.1"
                     /translation="MFNKIKIFFRKLNWFIKNYKVLYDSSEIVYGPLTYNTDGLATRN
                     NADFLKDEKFIKAYSKSYKTKSWEGFDIRWRAYIAYSFADLVKNIEGDFVECGVNKGG
                     LSIGIIEYINFNQLNKTFYLLDTFQGYDEKYLSTDEKNLGLKEQYNMYVDCYDDVVNT
                     FKEHPVKIIKGSVPETLTQCDTNKIAYLSIDMNCVLPEIEAIKYFWDKITIGGVIVLD
                     DYGFPNHINQKCAHDELAKVLGYNIISLPTGQGIIIKNK"
     gene            1319..1918
                     /locus_tag="PLS16_10745"
     CDS             1319..1918
                     /locus_tag="PLS16_10745"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNJ61604.1"
                     /translation="MILDFYKKYKRRKYLKSINQYIEMDKSSIITEDFRLSIPFAIKN
                     KKYLFIGKDNIILGNFQINNQEGIIKIGNNTQTNGDFICVHKIEIGDNVFIGWGTTLF
                     DNDSHPIEYKLRQLDMENQLKDLRSGKSFIENKNWSVVSSAPILIKNNVWIGMNVIIL
                     KGVTIGEGAIVAAGSVITKDVPDWTLVGGNPAKIIKNLK"
     gene            1915..2811
                     /locus_tag="PLS16_10750"
     CDS             1915..2811
                     /locus_tag="PLS16_10750"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNJ61605.1"
                     /translation="MNILIIGSEGFIGSNLKRYFQANGNSITALDIIEKKADNIDYFN
                     LCQTTLEKIFNHKQYDIVIFCAGNANVQKSFSAIDYDFISNTKFVHDILLHIRNFQKN
                     CKFLHISSAAVYGNPTQIPIMEKSPKNPISPYGFHKYQAELICKEFNTLFNIPTCSIR
                     PFSVYGPGQKKLLFWDIYQKAQNNKTIQLQGRGTESRDFIYIDDFVDAVNCIINNASF
                     NNDIINIANGNEVTIKECVETFIKVFDDTLEYEFNGINLIGNPLRWAADITQLSALGY
                     QQKISLKQGLEKYKAWIEENQF"
     gene            2787..>3487
                     /locus_tag="PLS16_10755"
     CDS             2787..>3487
                     /locus_tag="PLS16_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61606.1"
                     /translation="MDRRKSILIPTSGSYQNAASMIIYLLNIIKGLNNIVDDKNKPNI
                     ILYHDNETPIDEFKKINYPYLIYFNYKKDVSFIKRVFNLIARKYLNKNLFKTYSQKVD
                     YVFPALPDNSFVDANELVFWKADFQEKYLPHFFTVNDIKYSESFFTNLLEYQQSTLVL
                     SSQDAYKSFKQFYPNHQNPVFVLPFVSFLKIKEIDFHSIKEKFQIKKPYYIVCNQFWP
                     HKNHIRVLEAISQLK"
BASE COUNT         1353 a          416 c          516 g         1202 t
ORIGIN      
        1 tttagatgct aatcatttaa actataaaga attagccgga gaagattttg atatgtggat
       61 acaaattgca aaacacagta aaatctataa tatacccaag ccgatgcttc gatatagaat
      121 tcatcaaggc aatatgattc atagcaattg gtacaaatta gatgatggct tgtttagtat
      181 tatcaaagag caactcttct attattttca atcaaaaata actaatgatg atgttgaagc
      241 acattatagt ttgataaatt ttaatcgaat gaatcaactc gatgagttgc caaatgtcta
      301 tgaatggttg aagaaaatta taaatttgaa taatgaattt aaagtttata atccaattgc
      361 tttaaaaaag gttttgtcag aaagaatgac taaaaaatta atgagaatgt cagcaaatag
      421 ctttatgctc acaatttatt taataaaatg ctttaaccta cttggattta aaaatatctt
      481 tttcaatata aaccgctcga tttatattgt tttcctttct atgtttagaa taaaaattaa
      541 aaatgtttaa taaaataaaa atatttttta ggaagttaaa ttggtttata aaaaactata
      601 aggtactata tgacagttct gaaattgtat atggcccatt aacttataat acagatggat
      661 tagcgacaag aaataatgct gattttttaa aagacgaaaa atttataaaa gcatattcca
      721 aatcttataa aacaaaatca tgggaaggat ttgatattcg ttggagagct tatattgctt
      781 attcttttgc tgatttagta aaaaatatag aaggagattt tgtagaatgc ggtgtaaata
      841 aaggtggttt atcaatagga attattgaat acattaattt taatcaactt aataagactt
      901 tttatttatt agatacgttt caaggttatg acgagaagta cttatctact gatgagaaaa
      961 atttaggctt aaaagaacaa tacaatatgt atgtggattg ctatgatgat gttgtcaata
     1021 catttaaaga acatcccgta aaaattataa aaggtagtgt tcctgaaaca ctgacccaat
     1081 gtgatacgaa taaaattgca tacctttcta ttgatatgaa ttgtgtgttg cctgaaattg
     1141 aagccataaa atacttttgg gacaaaatta caattggtgg tgtaattgtg ttagatgatt
     1201 atggttttcc aaatcatatc aaccaaaagt gtgcacatga tgaattagcc aaagtattag
     1261 gttataatat tatttcactg ccaacaggac aaggaattat tatcaaaaat aaatagccat
     1321 gattttagat ttttacaaaa aatataaaag aagaaaatat ctaaagtcaa tcaatcaata
     1381 tattgagatg gataaatctt ccataataac tgaagatttt agactttcaa taccatttgc
     1441 aattaaaaat aaaaaatatc tttttattgg aaaagacaat attattttgg gtaattttca
     1501 gataaacaat caagaaggta ttattaaaat tggaaataat acacaaacaa atggagattt
     1561 tatttgtgtc cataaaattg aaattggcga taatgtattt attggttggg gaactactct
     1621 tttcgataat gattcacatc ctattgaata taaattaaga caattggata tggaaaatca
     1681 attgaaagat ttacgctcgg gtaaatcatt tattgaaaat aaaaattggt cagttgtgtc
     1741 atctgctcca attttaataa aaaacaatgt ttggattggt atgaatgtaa ttattttaaa
     1801 aggcgttaca attggagaag gtgcgattgt tgccgcagga agtgtcatta ctaaggacgt
     1861 tcctgattgg actttagttg gtggaaatcc tgcaaaaatt attaaaaatt tgaaatgaat
     1921 atactcataa ttggctcaga aggatttatt ggttcgaatt taaaacgcta tttccaagct
     1981 aatggaaatt caattactgc tcttgatatt attgagaaaa aagctgataa tattgactat
     2041 ttcaatttgt gccaaactac tttagaaaaa attttcaacc acaaacagta cgatattgta
     2101 atattttgtg ctggcaatgc aaatgtccaa aaatctttta gtgctatcga ttacgatttt
     2161 attagtaata caaaatttgt acatgatata ttacttcata taagaaattt ccaaaaaaat
     2221 tgtaagtttt tacatatttc aagtgctgct gtttatggca atccaacaca aattcctata
     2281 atggaaaaat cacctaaaaa ccctatttcg ccttatggtt ttcacaaata tcaagcagaa
     2341 ttaatttgta aagaatttaa tactttattc aatattccga cttgtagtat tagaccattt
     2401 tctgtttatg gtccaggaca aaaaaaactt ttattttggg atatttacca aaaggctcaa
     2461 aataataaaa ccatacaatt acaaggaaga ggaacggaat ccagagattt tatttatata
     2521 gatgattttg ttgatgctgt taattgtatt ataaataatg cttcatttaa taatgatatt
     2581 ataaatatcg ccaacggaaa tgaagttacg ataaaagaat gtgttgaaac atttattaaa
     2641 gtatttgatg atactttaga atacgaattt aatggtatta atttaattgg aaatcctttg
     2701 agatgggctg ctgatattac acaattaagt gcattaggct atcagcaaaa aataagttta
     2761 aaacaaggac tagaaaaata taaagcatgg atagaagaaa atcaatttta attcctacgt
     2821 cgggtagtta tcaaaatgca gcatctatga ttatctattt gctcaatatt attaaaggat
     2881 tgaacaatat tgtagatgat aagaataagc caaatattat tctgtatcac gataacgaaa
     2941 cacccattga tgaatttaaa aaaatcaatt atccatactt aatttatttt aattataaaa
     3001 aagatgtatc atttatcaaa cgagttttta acctaatagc tcgtaaatat ttaaataaaa
     3061 atttattcaa aacatatagc caaaaagtgg attatgtttt tccagctctg cctgacaata
     3121 gttttgttga tgcgaatgaa ttagtatttt ggaaagctga ttttcaagaa aaatatttac
     3181 ctcatttttt tacagtaaat gatataaaat attcagaaag tttttttaca aaccttttag
     3241 aatatcaaca atctacatta gtattaagta gtcaagatgc ttataaaagt tttaagcaat
     3301 tttatcctaa tcatcaaaat ccagtttttg ttctgccttt tgtatccttt ttaaaaatta
     3361 aagaaattga cttccattca ataaaggaaa aatttcagat taaaaagccc tattatattg
     3421 tgtgcaatca attttggcca cacaaaaatc acatcagagt tttagaagca attagtcaat
     3481 taaaaag
//
LOCUS       DAOQCW010000158         3401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_15036_length_3401_cov_5.298267, whole genome shotgun sequence.
ACCESSION   DAOQCW010000158 DAOQCW010000000
VERSION     DAOQCW010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15036_length_3401_cov_5.298267"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1367
                     /locus_tag="PLS16_10760"
     CDS             <1..1367
                     /locus_tag="PLS16_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNJ61607.1"
                     /translation="KATRQILSETLIDIHQVKGLAFTTQAMGVIPVDSHGNVLYRNIT
                     WVDGRAEEQAKKAMQYFGHKRIFKAIVGTELTGKDVIPKIIWLKDKQPDIYNNTHKIL
                     DVNGYLKMKCTGKMVAEWSGACSYGFDVKKKDWERLFFKLCKIDLNKLPDLVRSIDLV
                     GTLTNQAAEELGLPKQVEVYGGCDDTQSAAVGTTAIGEGEPHIYVGTSAWIGVATEKA
                     PKFKNGAFTLQSADPAKNLVVGITESAGINTQWLINNFYSEELKHKKDQEFFAYLEDE
                     INKTPAGADYLIMTPWFLGERCPVSTTTTRSTLFNLTHQHTKAHIARANFEGIAMNLR
                     WSIQNFERDFGFKIERIKITGGGSRNHTFMQILADVLGKTIITTSQPLNAGALGAAMC
                     AMVGSKVLNGFNDIEKIIQTQDEFKPNINNQPIYDELFSTYQSVYHQLEKSYQSINKK
                     RFEL"
     gene            1385..1963
                     /locus_tag="PLS16_10765"
     CDS             1385..1963
                     /locus_tag="PLS16_10765"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNJ61608.1"
                     /translation="MTVQEAKEKVCAAGKQLLKEGLVARTWGNVSIKVNERQMVITPS
                     GRKYDELTPEDMVLVDFYTLKYEGIKPSSELKLHCEVYKTRPHIESVIHTHQMYASIV
                     AAARKDVIVSDLEHQKILGGNIIKAAPYALPNTKKITVETARAIENSNAALMSNHGVV
                     CIGKDIEDVFVVARTLEAACALFVENQKNKIA"
     gene            1960..2562
                     /locus_tag="PLS16_10770"
     CDS             1960..2562
                     /locus_tag="PLS16_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61609.1"
                     /translation="MTSLEAKNIILETYNYTTQQSAGYQTWGKVDILQIAVRINQQEY
                     AFSKDGVNLKSITENDIIISNDKTNLFGKLFHSKKKIEVILITKQTYAAQVKEEIPPI
                     LDDQAQLLGVSIRIAKNENNLSSALCSRYAAIFADGRSICIGTNLEDAYVAAQLLEKT
                     SKAFVEAKVLGGAKSINKIEAWLMQQFYQLKYSKEAKKNK"
     gene            complement(2566..2982)
                     /locus_tag="PLS16_10775"
     CDS             complement(2566..2982)
                     /locus_tag="PLS16_10775"
                     /inference="COORDINATES: protein motif:HMM:NF014346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNJ61610.1"
                     /translation="MKQKITILLTLLSFVFIANSFAMKMKKKETSQFKNLQVLPNDID
                     MESLKGIMKSFNTALGVKCDHCHAASAINPEELDFASDAKFQKEIARGMMKMTKEINE
                     NYFAKYPHDGMVNQIECMTCHNGKAEAHHIQKPVAP"
BASE COUNT         1280 a          472 c          637 g         1012 t
ORIGIN      
        1 gcaaagctac ccgacagatt ttaagtgaaa cactaattga tattcatcaa gttaaaggat
       61 tagcctttac aactcaagcg atgggcgtta ttccagtgga ttcgcatgga aatgtattgt
      121 atagaaatat tacttgggtg gacggaagag cggaagaaca agctaaaaag gcaatgcaat
      181 atttcggcca caagcgtatt tttaaagcaa ttgttggaac tgagctaaca ggaaaagatg
      241 taattccaaa aattatttgg ttaaaagata aacaacctga tatttataac aatacgcata
      301 aaatacttga tgtcaatggt tatctaaaaa tgaaatgcac tggaaaaatg gtggctgaat
      361 ggtcgggtgc ttgttcttat ggttttgatg taaagaaaaa agattgggag cgtttgtttt
      421 tcaaattgtg taagattgat ttgaataaat tacctgattt agttcgttca atagatttag
      481 taggtacttt aaccaaccaa gcagcagagg aattgggttt acccaaacaa gtcgaagttt
      541 atggtggttg tgatgataca caaagtgcag cagtgggaac aactgcaata ggagagggcg
      601 aaccacatat ttatgtaggc acatctgctt ggattggcgt agccactgaa aaagccccaa
      661 aatttaaaaa tggagcattt accttacaaa gtgctgaccc tgcaaaaaat ttagtagttg
      721 gtatcacaga atctgctgga attaatacgc aatggctgat taataatttt tattcggagg
      781 aattaaaaca taaaaaagac caagaatttt ttgcctattt ggaagatgaa ataaacaaaa
      841 cgccagcggg tgcagattat ttgataatga caccttggtt tttgggcgaa cgttgtcctg
      901 taagcacgac caccactcgt tctaccttgt ttaacctaac acatcaacat acaaaagcac
      961 atattgcaag agccaatttt gaagggattg ctatgaattt acgttggtca attcaaaact
     1021 ttgaaaggga ttttggattt aaaattgagc ggattaaaat tactggtgga ggaagtcgta
     1081 atcatacatt tatgcagatt ttagctgatg ttttaggcaa aacaattatt accacttctc
     1141 aaccgctgaa tgctggtgct ttaggtgctg caatgtgtgc aatggttgga agtaaagtat
     1201 tgaatggttt taatgatatt gaaaaaatta ttcagacgca agatgagttt aagccaaata
     1261 tcaataatca acctatctat gatgaactat ttagtactta tcagtccgtg tatcatcaat
     1321 tagaaaaaag ttatcaatcg attaataaaa aaagatttga attgtaaaaa ataaataagg
     1381 acgtatgacg gttcaagaag caaaagaaaa agtttgtgca gcaggaaaac agttattaaa
     1441 agaaggctta gtagctagaa cttggggcaa tgtaagtatt aaagtgaatg aaagacaaat
     1501 ggtgattaca ccaagtggta gaaaatatga tgaactcaca ccagaagata tggttttagt
     1561 tgatttttat actttgaaat atgaaggtat aaagccatca tctgaattaa aattacattg
     1621 tgaagtttat aaaactagac cacatataga atctgtaatt catacccacc aaatgtatgc
     1681 atctatcgtt gctgcggcac gtaaagacgt tattgtaagc gatttggagc atcaaaaaat
     1741 attgggtgga aatatcataa aggcagcacc ttatgccttg ccgaatacca aaaagattac
     1801 agttgaaact gctagagcca tagaaaattc taatgcagcc ttaatgtcca accacggagt
     1861 tgtttgtatt ggaaaagata ttgaagatgt gtttgtagtt gcaagaacat tggaagccgc
     1921 atgtgcatta tttgtagaaa accagaaaaa taaaattgca tgacaagcct agaagctaaa
     1981 aatattattt tagaaactta taattatacg acacaacaga gtgcaggata tcaaacttgg
     2041 ggaaaagtag atattttaca aattgcagta agaattaatc aacaggaata tgcattttct
     2101 aaagatgggg taaatttaaa atctataacc gaaaatgata ttataattag taatgacaaa
     2161 actaacttat ttggaaaatt atttcacagt aagaaaaaaa tagaagttat tttaattaca
     2221 aagcaaacgt atgctgcaca agttaaagaa gaaataccac caattttaga tgaccaagct
     2281 caattattag gtgtgagtat tcgaattgct aaaaatgaaa ataatttaag ttcagcttta
     2341 tgcagtagat atgcagctat ttttgcagat ggtcgcagta tttgtatcgg tacaaactta
     2401 gaagatgctt atgtcgccgc acaattattg gaaaaaacat caaaagcatt cgtcgaagcc
     2461 aaagttttgg gcggagcaaa atctattaat aaaattgaag cttggttaat gcaacaattc
     2521 tatcaattga aatattccaa agaagcaaaa aagaataagt aatttttacg gagcaactgg
     2581 tttttgaata tgatgtgcct ctgcttttcc attgtggcaa gtcatacatt caatttgatt
     2641 caccatacca tcgtgtggat attttgcaaa ataattttca ttaatttctt tagtcatttt
     2701 catcatgcca cgagcaattt ccttttgaaa ttttgcatca ctagcaaaat ctaattcttc
     2761 agggttgatt gcacttgcag catggcaatg atcacatttt acacctaatg ccgtattaaa
     2821 agatttcata attcccttta atgattccat gtcaatatca tttggaagca cttgcaaatt
     2881 tttaaactga gaagtctctt ttttcttcat cttcatagca aatgagttag caataaatac
     2941 aaatgacaat aaagtaagta gaattgtgat tttttgtttc ataagataga tgtttagttg
     3001 tttaaagtta aaccaaagaa acaatataaa caagaaaata ttaaaatgag tatttagtag
     3061 aaagtattga gagttgagaa tcaagattat tagagccaag agccaagacg aaaacgaatt
     3121 gagaattaaa aattgaaaat tgagaattaa aagctaacaa ctaaccactg tataaaaatc
     3181 tttgtgaact ctgtgtaaaa acttagtgtc ctttgtggtt aaaaaagtat tgagaatcaa
     3241 gatttttaga accaagagtc aagaaccaag acgaaaacga attgagaatt aaaaattgag
     3301 aattaaaaat tgagaattaa aaactaaaaa ctaaaagcta acaactgtga aaactttgtg
     3361 aattctgtga aaaaacttag tgtcctttgt ggttaaaaaa g
//
LOCUS       DAOQCW010000159         3384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_15179_length_3384_cov_5.911986, whole genome shotgun sequence.
ACCESSION   DAOQCW010000159 DAOQCW010000000
VERSION     DAOQCW010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15179_length_3384_cov_5.911986"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="PLS16_10780"
     CDS             complement(<1..863)
                     /locus_tag="PLS16_10780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HNJ61611.1"
                     /translation="MTRVNINKVAKPFLKWAGGKTQLINDIELLLPKDLIKTNFTYIE
                     PFVGSGAVLFWMLNNFPNMKKAVINDINADLINTYKTIASKPNELIALLDILQSEFHA
                     LEGNEDNKKCYYYQKRELYNLRSESQIAQSALFIFLNRTCFNGLYRVNRKNEYNVPMG
                     GYNKPTICDKENILSVSQALQRVEILCGDYEETLNYADNNTLFYFDPPYKPLSETSSF
                     NSYAKDDFNDNEQIRLKEFCHKLDILNHTWILSNSDVKGKDENDNFFDDLYADFNIQR
                     VEAKRNINANP"
     gene            1126..>3384
                     /gene="uvrA"
                     /locus_tag="PLS16_10785"
     CDS             1126..>3384
                     /gene="uvrA"
                     /locus_tag="PLS16_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNJ61612.1"
                     /translation="MIENQLPIITKEELTHINIFGAKVHNLKNIDVHIPRNQFVVITG
                     LSGSGKSSLAFDTIFAEGQRRYLDSFSNYLRNFIGNMERPDVEKISGLSPVISIEQKT
                     TNRNPRSTVGTTTEIYDFLRLLFARAGEAYSYNTGKKMTKFSEDQIIENISTKYKDKK
                     IAILAPLVRGRKGHYRELFEDIRKKGFLRVRIDGETQEIQPKMQVDRYKIHDIEAVID
                     RLTVQEKELQRIRKSVQEALKLGDNLIFIEDLETKKVSIYSKSLMCVETGISYEIPSP
                     NTFSFNSPYGYCPTCKGLGVLNAPDLKKIIPDNNKSLAEEGIVPLGEVRDTYSFKIID
                     QYLKRYKVNQQTPIKDYPKELLDIILYGSDEKIKIEAKDNKDRKTNNTHWQLSFDGVF
                     NQIERTMEDGNAGNALQNWASQYISTIQCPDCQGTRLKNESLWFKLDNKNIAEVASMD
                     IQELKTWVEQLESKLDAQQKLIAKDILKELRDRIGFLLDVGLSYLNLNRPTGTLSGGE
                     SQRIRLASQIGSQLMGITYILDEPSIGLHQRDNHRLIDALRNLTDIGNTVIVVEHDKD
                     IMLASDYIIDMGPAAGEYGGTIVAQGTPKDFIQQDALTAQFLNNKIRIELPKERRKGN
                     GKFLELSGASGNNLKNVTIKIPLGSFVGISGVSGSGKSTLINETLYRILNKHFYRTPY
                     QPMAYKKIKGLEHIDKVIEIDQSPIGRTPRSNPATYVGVFDAIRNLYASLPESQIRGF
                     KPGRFSFNVKGGR"
BASE COUNT         1206 a          512 c          561 g         1105 t
ORIGIN      
        1 tcaggatttg cattgatatt tctttttgct tcaactcgtt gtatattaaa atcagcatat
       61 aaatcgtcaa aaaaattatc gttttcatct ttacctttca catccgaatt gcttaatatc
      121 caagtatggt ttaaaatgtc taatttgtgg caaaattctt ttaaacgaat ttgttcgtta
      181 tcgttaaaat cgtcttttgc ataagaattg aaacttgaag tttcgcttaa tggtttgtag
      241 ggtggatcga aataaaatag ggtgttattg tctgcataat tcagtgtttc ttcatagtcg
      301 ccacaaagga tttccactct ttgcaatgct tgacttactg ataaaatatt ttctttatcg
      361 caaatcgttg gtttattata accacccatc ggcacattat attcgttctt gcggttcact
      421 cgatacaatc cattaaaaca agtccgattt agaaaaataa acaaagcaga ctgagcaatt
      481 tgtgattcac ttcttaaatt atacaattct ctcttttggt aatagtaaca ttttttatta
      541 tcctcatttc cttctaatgc atgaaattca ctctgtaaaa tatcaagtaa agcaattagt
      601 tcatttggct tacttgcgat cgttttataa gtattaatta aatcagcatt gatgtcattg
      661 attacggctt ttttcatatt ggggaagttg ttcaacatcc aaaataatac agcaccactc
      721 ccaacaaaag gctcaatata ggtaaaattt gttttgatta aatcctttgg cagaagcaat
      781 tcaatatcgt ttatgagctg agtttttcct ccagcccatt ttaagaaagg tttcgccact
      841 ttattaatat tcactcttgt cattccaata tgtatgtttc aaatttacaa aattatcttg
      901 atgaaagtct atctgaaaat ggaaagttgg tttttaggat gaaagtacaa aagttgttgt
      961 atctttctaa aaagatttac agttttcatt tttacacatt tttttagtta ttaatgtata
     1021 aagctatttc aaaacaagga aataaaacat atagccgcta ctttgtaatt catctgaagt
     1081 ctaaattatt taatttctac ctaattttga attacttttg ttattatgat tgaaaatcaa
     1141 ctacccatta ttacaaaaga agaacttaca catattaata tatttggagc taaagtacat
     1201 aatctaaaaa atatagatgt tcatattccg aggaatcaat ttgttgttat cacaggcttg
     1261 agtggtagtg gtaaatcttc attagcattt gatactattt ttgccgaagg acaacgacgt
     1321 tatttggata gtttttcaaa ttatcttagg aatttcatag gaaatatgga gcgacctgat
     1381 gtcgaaaaaa tatctggatt atcgccagta atttctatag aacaaaaaac aaccaataga
     1441 aacccaagaa gtacagtagg cacaactaca gaaatctatg attttctacg tttacttttt
     1501 gcacgagctg gcgaggctta ttcctataat actggaaaga agatgacaaa attctctgaa
     1561 gaccaaatta tagaaaatat ttctactaaa tataaagata agaaaattgc tattcttgct
     1621 cctttggtaa gaggtcgaaa aggacattat cgtgaattgt ttgaagatat aagaaaaaag
     1681 ggctttttac gagttcgtat agatggcgaa acacaagaaa tacagcccaa aatgcaagta
     1741 gatcgatata aaattcacga tatagaagcg gtaatagaca gattaactgt acaagaaaag
     1801 gagctacaac gcatcagaaa atctgtacaa gaagctttaa aattaggaga caatcttatt
     1861 tttattgaag acttagaaac taaaaaagtt agcatttata gtaaatcttt gatgtgtgtt
     1921 gaaactggta tctcttatga aattccttct cctaatacat tttcgtttaa ttctccttat
     1981 ggttattgtc caacttgcaa aggattaggc gttttgaatg ctccagattt aaaaaaaatt
     2041 atacctgata acaacaagtc tttagctgaa gagggtattg taccgcttgg tgaggttaga
     2101 gatacttact ctttcaaaat catagatcaa tatttaaaaa gatataaagt taatcaacaa
     2161 acaccaataa aagattatcc taaagaatta ttagatatta tattgtatgg gagtgatgaa
     2221 aaaataaaaa tcgaagctaa agataacaaa gaccggaaaa caaataatac acattggcaa
     2281 ctttcatttg atggtgtttt caatcaaata gaacgaacaa tggaagatgg aaatgctggc
     2341 aatgcactgc aaaattgggc atctcaatat atatcaacaa ttcaatgtcc tgattgtcaa
     2401 ggtacaagat tgaaaaatga atcgctttgg tttaagctag ataataaaaa tattgctgaa
     2461 gttgcttcaa tggatattca agaattaaaa acttgggtgg aacaactaga aagcaaactt
     2521 gatgcacaac aaaaattaat tgcaaaagat attttaaaag aattacgaga tagaattggc
     2581 tttttattag atgttggact aagttatttg aatttgaatc gtccgacagg tactttatct
     2641 ggaggtgaat cccaaagaat tcgtttggca tctcaaattg gttcacaatt aatgggtatc
     2701 acttatattt tagacgaacc gagcataggt ttacaccaaa gagataatca tcgattaatt
     2761 gacgctcttc gaaatttgac ggatatcgga aatactgtaa ttgtcgtgga gcatgataaa
     2821 gatattatgt tagcatcgga ttatataatc gatatgggtc cagcagctgg agaatatggc
     2881 ggaacaatcg ttgcacaagg cacacctaaa gattttatac aacaagatgc cctaactgct
     2941 caatttctaa acaataaaat tcgcatagag ctaccaaaag aaaggagaaa aggcaatggg
     3001 aaattccttg aattatctgg agcaagtggt aataatctaa aaaatgtaac gataaagata
     3061 cctttaggct catttgtcgg catttcaggc gtttcaggtt caggaaaatc tactttaatc
     3121 aatgaaacat tataccgaat actcaataag catttttata gaacaccgta tcagccgatg
     3181 gcttataaaa aaatcaaagg cttagaacat attgataaag tgatagaaat tgaccaatca
     3241 ccaattggtc gaactcctag aagcaatcca gcaacttatg ttggagtttt tgatgccatt
     3301 cgtaatttat atgcttcttt acctgaatct caaattcgtg gatttaagcc tggacgattt
     3361 tcatttaatg taaaaggagg tcga
//
LOCUS       DAOQCW010000160         3273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_16107_length_3273_cov_5.606588, whole genome shotgun sequence.
ACCESSION   DAOQCW010000160 DAOQCW010000000
VERSION     DAOQCW010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16107_length_3273_cov_5.606588"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(302..1210)
                     /locus_tag="PLS16_10790"
     CDS             complement(302..1210)
                     /locus_tag="PLS16_10790"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNJ61613.1"
                     /translation="MEELQACPCCTSKNFKHNIEVKDYYVSQETFSIALCNDCGFQFT
                     NPRPEVDKIGKYYDSVNYISHNSNDDSLFHKVYRFIRNIMTKKKINTLRQYVHKESAD
                     LSLLDYGAASGDFLAACQLNNITKVIGIEQDETCRNNAKLNYNLDIKSPKELMDIQAE
                     SIDIITLWHVLEHVHNIDETLLHFHRILDKNGVLAISVPNIISYDSIYYKQFWSAIDV
                     PRHIYHFEPKTIEILMQRLGFQLMGTMKMPFDSYYISLHSEYYQKRSGIMAIIRAIVI
                     GFISNWKARKTNNYSSLTYFFKIKKV"
     gene            complement(1215..1967)
                     /locus_tag="PLS16_10795"
     CDS             complement(1215..1967)
                     /locus_tag="PLS16_10795"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HNJ61614.1"
                     /translation="MFKKFLNFFIFAYARIQNLPYFWFSRLINKGYHIIVPSATYAPW
                     IHDKFFLKTFAEAKKNSLLNFYQAWELWQLAENTSGKEGNILEVGVWRGSSSIMMGTK
                     LKQIGSNKKIFACDTFEGVVKAGTKEDNFYVGGEHKDTSLEFVKNHIENNFHLSNVQL
                     LKGIFPDDTGHLVTNEKFCLVHIDVDAYISAKDIMTWVWDRLTIGGVVVFNDYGFPLT
                     KGITLLVNEYLQRGDAVVVHNLNGNGIIVKIK"
     gene            complement(1979..2815)
                     /locus_tag="PLS16_10800"
     CDS             complement(1979..2815)
                     /locus_tag="PLS16_10800"
                     /inference="COORDINATES: protein motif:HMM:NF025024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HNJ61615.1"
                     /translation="MNFLNNKTVHELLDFGKENHDAYINAKPFPSGYYDNLFNPEMLR
                     EIIAEFPEIGKVKEDIQYTNPNELKLATKGEYRFGEKTKAFIHFLNSQPFLEFLQNLT
                     GIKETLIPDPYLEGGGFHEIKPGGYLKMHVDFHKNQRTNLDRRLNILVYLNEDWKDEY
                     GGHFELWEKDMSKCVTKIAPLFNRLAMFSTTDFSWHGLPDPLTCPPDRSRRSIALYYY
                     TNGRPEGEVNVGDKGRITTTFATRKGQDSTKMTIFNSFVNFANDILPPFLVRLIKKFR
                     NT"
     gene            complement(2959..>3273)
                     /locus_tag="PLS16_10805"
     CDS             complement(2959..>3273)
                     /locus_tag="PLS16_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNJ61616.1"
                     /translation="KETENSFVVEAAIPGVKKEDVKVELNENVLTISSETKKEENESN
                     DKYTRREYSYSSFKRSFTIDEDAINTEGIDAKFENGVLAIILPKKEAKAPEQKVKTIN
                     VV"
BASE COUNT         1110 a          570 c          413 g         1180 t
ORIGIN      
        1 attggttgtc caaatgattt ctataataac tttaataaaa aagaaacgac aactaaaaat
       61 atgaatagca ataaacctca aaaaaacaag aaacctgtga gcaatccaat gggaccgcag
      121 tagttagagc ccagattgtt agagccaaga atcaagactt tatggagtat taagtagaaa
      181 gtagtgagta ttgagaaatt tattatttca ttattacatt aatttactaa tcattaaaaa
      241 aattctgcta atcttcaaat cctataaatc ctgattcaga caattaggca ataaacattt
      301 cttatacttt ctttatctta aaaaaataag ttaggcttga ataattattt gtttttctag
      361 ctttccaatt gctaataaat cctataacaa ttgcacgaat gattgccatt atgccactac
      421 gtttttgata atattcacta tgtaaactga tataataact atcaaaaggc attttcatag
      481 tacccattaa ttgaaaccct aaacgttgca ttaaaatttc aattgtttta ggttcaaaat
      541 gataaatatg tcttggcaca tcaatagctg accaaaactg tttataatat atagaatcat
      601 aagaaattat attcggaaca ctaattgcca aaaccccatt tttatctaaa attctgtgaa
      661 aatgcaataa ggtttcatca atattatgta catgttccaa aacatgccaa agtgtaataa
      721 tatcaatact ttcagcttga atatccatta actctttagg agattttata tctaaattat
      781 aatttagttt tgcgttattt ctacaagttt catcttgttc aattccgatg acttttgtga
      841 tattattgag ctgacaagct gccaaaaaat ctcccgatgc tgctccataa tctaataaac
      901 ttaggtctgc actttcttta tgtacatatt gacgaagtgt attaatcttc tttttcgtca
      961 tgatatttct gataaaacga taaaccttat gaaataaaga atcatcattc gaattatggg
     1021 aaatataatt aacagagtca taatatttcc ctatcttatc aacttctggt cttggatttg
     1081 taaattgaaa gccacagtcg ttacataaag caattgaaaa agtttcttgg gaaacgtaat
     1141 aatctttaac ctcaatatta tgtttaaaat ttttactggt acaacacgga caagcttgta
     1201 attcttccat tatactactt tatttttaca ataataccat tgccatttaa gttgtgtacc
     1261 actactgcat cacctcgctg taaatattca ttaactaaaa gtgttatacc ttttgtaagt
     1321 ggaaatccat aatcattaaa aacaactaca ccaccaattg ttaatctgtc ccaaacccaa
     1381 gtcataatat cttttgctga aatataggca tcaacatcaa tatgcactaa acaaaatttt
     1441 tcatttgtta ccaagtgtcc tgtgtcatct gggaaaatac cttttaataa ttgaacatta
     1501 ctcaaatgaa aattgttttc tatatgattt tttacaaatt ccaatgatgt atccttatgt
     1561 tctccaccaa cataaaaatt atcttcttta gttccagctt taaccactcc ttcaaatgta
     1621 tcgcaggcaa atattttttt attactacca atttgtttta gttttgttcc catcataata
     1681 gaacttgaac cacgccaaac gccaacttct aaaatattac cttctttgcc agaagtattt
     1741 tctgctaatt gccaaagttc ccaagcttga tagaaattca atagcgaatt tttctttgct
     1801 tcagcaaatg tttttagaaa aaatttatca tgtatccatg gtgcataagt cgctgatgga
     1861 acgataatat gatagccttt attgattaat cttgaaaacc aaaaataagg taaattttgt
     1921 attcttgcat aagcaaaaat gaagaagttt aagaactttt tgaacatgaa tttcttgttt
     1981 aagtatttct gaattttttg attaatctta ctaaaaatgg aggtaaaata tcgtttgcaa
     2041 agttgacaaa tgaattaaaa atagtcatct tggtagaatc ctgacctttt cttgtggcaa
     2101 aggttgttgt aattctacct ttatcgccca catttacctc tccttcaggt cgtccgttgg
     2161 tgtaataata taaagcgatt gaacgtctgc ttctatctgg cggacaagtt agtgggtctg
     2221 gtaagccatg ccaagaaaaa tctgttgtag aaaacatcgc taatcgatta aatagtggag
     2281 caattttagt aacacatttg ctcatatctt tttcccaaag ctcaaaatgt ccgccatatt
     2341 catctttcca atcctcattc aagtaaacta atatatttaa acgtctatcc aaatttgtcc
     2401 tctggttttt atgaaaatca acatgcattt tcaaataacc tccaggttta atttcatgaa
     2461 atcctccacc ttccaaatat ggatcaggaa taagtgtttc ttttattcca gtcaaatttt
     2521 gtaaaaattc caagaatggt tgcgaattta gaaagtgaat aaatgctttc gttttctcac
     2581 caaaacgata ttctcctttg gtggctaatt tcaactcatt aggatttgta tattgtatat
     2641 cttcttttac tttgccaatt tcaggaaact cagcaataat ttcacgcaac atttctggat
     2701 taaacaaatt atcgtaataa ccacttggaa atggttttgc attaatatat gcatcatgat
     2761 tttcctttcc aaaatctaaa agctcatgta ccgttttatt atttaaaaaa ttcattcttt
     2821 aattataaaa tacaaaaata aataacctat tggaaaatca gtactaattc aattaataaa
     2881 tgtgtacaaa ctataccaat acagcacaaa ataaaaaagc ggtcattact gaccgctcca
     2941 aaaacacttc ttattaaact aaactacatt gatagttttt actttttgct ctggcgcttt
     3001 ggcttctttt tttggtaata ttattgctaa tactccattc tcaaatttag catcaattcc
     3061 ttccgtatta atggcatctt catcgatagt aaagctacgt ttgaaactag aatagctata
     3121 ctctcttcga gtatatttgt cattgctttc gttttcttct tttttcgttt ctgatgaaat
     3181 agtcaataca ttttcattca attctacttt tacatcttct ttttttacac ccggaatggc
     3241 agcctcaact acaaaagaat tttctgtttc ttt
//
LOCUS       DAOQCW010000161         3123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_17637_length_3123_cov_4.616362, whole genome shotgun sequence.
ACCESSION   DAOQCW010000161 DAOQCW010000000
VERSION     DAOQCW010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17637_length_3123_cov_4.616362"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PLS16_10810"
     CDS             complement(<1..287)
                     /locus_tag="PLS16_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61617.1"
                     /translation="MEGADKFNKDIEKSADVILDVAKANKIADQSSDELAKSIGQTAN
                     QLQILGKEGKTSTDEFNKLNTSLKGQISSYEKVSTGATSMRSGIRATREELA"
     gene            complement(492..1067)
                     /locus_tag="PLS16_10815"
     CDS             complement(492..1067)
                     /locus_tag="PLS16_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61618.1"
                     /translation="MIKKIKNLFKKKTDIVLLHVHTFNDGTKLFTYREEDLGKVSSRY
                     YRNIMEANNYLHTFLLTKEQWTTAVNELKKRSTEALASNNNKEIVQTLLDINSTLDWF
                     IEKSSTLKNANETILELMFCMFFLLEDEAPTGYSDSFNQRKIELLNSNLEVRDFFLSN
                     LKRNLPNLAPLSDYDLKLQMIINKMTAEVLT"
     gene            complement(1151..2629)
                     /locus_tag="PLS16_10820"
     CDS             complement(1151..2629)
                     /locus_tag="PLS16_10820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HNJ61619.1"
                     /translation="MVLQKKKLNLGIDSSTILDTYRHILEPNDIDIEFLYGGRDSGKS
                     HFTAQKLIIDCLQLPYFRCIMIKKTHESIKDSQWQTIKDIVEDWGIGHLFIFKTAPLE
                     IICIVNNNKFISRGCDNPTKLKSIRNPSHAWYEEGDQISLEDFTTITTTLRTSIGKVK
                     QYFLFNPELPKGVTDKKDFWLYKNYFSHTNDKNFTKEIEYEIRGKVYKTKYRATHSTY
                     IDNLANVTADRISMYENLKITNPSKYLPYCLGEWGQYSNEVPFFYGYNKMKHFAHTNY
                     EPKEEYYIDIGFDFNITPCVCVIGQFDRENQIYHIFDTIMQDHTNIYNLSSLQAVCRK
                     IKEKYLDSGYYLRSRIRITGDASGRSGSADRQVKQNYYYTIQKELQLLPQQFYVRGAN
                     LPHTVSGEIINEVFVNMRNNALILHNQDLLERDIETSFPDAKQSLNEAKAKFGLHVVD
                     AWRYLMDFWFNFDKSNRTFVSKNHKKFTELIIKNVNNFNHYG"
     gene            complement(2629..>3123)
                     /locus_tag="PLS16_10825"
     CDS             complement(2629..>3123)
                     /locus_tag="PLS16_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61620.1"
                     /translation="KQILQLLREGYATLDILGVMSSKWELPETTFYNHLKEAKETYQK
                     ELLSQQKAIEEVREQEVKKVAQKGLKSKFERDLELQNDIEYYQDVLSGKIKTTFILGQ
                     KVSATESLPIQIVLLIQKTINDLRIELSKRLGEYATTQVELTNKNFIPDEVVITIIDG
                     KRVD"
BASE COUNT          903 a          586 c          442 g         1192 t
ORIGIN      
        1 gcaagttcct cacgtgtggc tcttatgcct gaccgcatac ttgtagcacc agtagatact
       61 ttctcgtagc ttgatatttg accttttaaa gaggtattta gcttgttaaa ttcatcagtg
      121 gaggtcttac cctccttacc taatatttgt aattgatttg ctgtttgtcc aatggatttt
      181 gctaactcat cagaactttg gtctgctatc ttattagcct tagccacgtc aagaatgaca
      241 tcagcagact tttcaatatc tttgttgaac ttatcagccc cctccacttg aaattttacg
      301 attatatttt cattagccat cttccctatt ttgtttatca aagttagcct ttttttcttt
      361 catttcttta ataaaattat agaggtaaat aaaataatgc tcaatatcta aatcttctat
      421 ctctttcatt gatagattat aatgatgtga taaccttaaa ataagagtat ctatattatt
      481 tttaaatgtt attatgtcaa tacttccgct gtcatcttgt tgattatcat ttgaagtttc
      541 aaatcgtaat cggataaagg agctaagttt ggtaaatttc gtttcagatt gctcaaaaaa
      601 aaatctcgaa cttctaaatt tgaattaaga agctcaattt ttctttgatt aaaactatct
      661 gaatagcctg taggtgcttc atcttcaaga agaaaaaaca tacaaaacat aagctctaaa
      721 atcgtttcat ttgcattttt taatgtgctt gatttttcaa taaaccaatc taaggtgcta
      781 ttaatatcaa gcaacgtttg tactatttct ttattattat tagaggctaa cgcttcggtg
      841 cttcttttct ttagttcatt aaccgctgtc gtccattgct cctttgtcag taagaaagtg
      901 tggaggtaat tgtttgcctc cataatgtta cgatagtacc ttgacgacac ttttcctaaa
      961 tcttcctccc tgtatgtaaa tagctttgtg ccatcgttaa acgtgtgaac gtgcaaaaga
     1021 acaatgtcag tctttttttt gaatagattt tttattttct ttatcatctt tcattttttt
     1081 aattatattt tgtgcttcaa tttgcttttc caaatttagc atatttttct tacttaataa
     1141 ctcttttagc ttatccatag tggttaaaat tattaacatt cttaattatt aattctgtaa
     1201 actttttatg gttcttactt acaaatgtac gattactttt gtcaaaatta aaccaaaaat
     1261 ccataagata cctccacgca tctacaacgt gcaatccaaa tttagcctta gcttcattta
     1321 acgactgctt agcgtcagga aacgacgttt caatatctcg ttccaataag tcttgattgt
     1381 ggagaattaa ggcattattt ctcatattaa caaacacttc gttaattatt tcgccactca
     1441 cagtatgcgg taaatttgcc ccccttacat agaactgctg tggtagtagt tgcaattctt
     1501 tttgaatagt atagtaataa ttttgtttta cttgcctatc cgcagatccg cttctaccac
     1561 ttgcatcgcc cgtaattctt atacgacttc taagatagta gccactatca aggtattttt
     1621 cttttatttt tctgcacacc gcctgtaaac tactaagatt atagatatta gtatggtctt
     1681 gcattatagt atcgaatatg tgatatattt gattttctct atcaaactga ccgataacgc
     1741 aaacgcaagg agtaatgtta aagtcaaatc ctatgtcaat atagtattct tcctttggct
     1801 cataattggt atgagcaaaa tgtttcattt tgttgtagcc atagaaaaaa ggcacttcat
     1861 tactatactg cccccactcg ccaagacaat acggtaagta ctttgacgga tttgtaattt
     1921 ttaaattctc atacatcgaa atcctatccg ctgtaacatt tgcgaggttg tcaatgtacg
     1981 ttgaatgagt agcacgatat ttagttttat agactttacc acgaatttcg tactcaattt
     2041 ccttcgtaaa attcttatcg ttggtatgag aaaagtagtt tttgtacaac caaaaatcct
     2101 ttttatcagt tacacctttt ggtagttcag gattaaaaag aaagtactgc tttacttttc
     2161 ctattgaagt tctaagcgtt gtggtaatag tcgtaaaatc ttcaaggcta atttggtcgc
     2221 cctcttcata ccaagcgtgt gaaggatttc ttatagattt taacttagta gggttatcac
     2281 accccctgct aatgaatttg ttattattta cgatgcaaat tatctccaaa ggtgcggttt
     2341 tgaatatgaa taaatgccca attccccaat cctccactat atctttaatc gtctgccatt
     2401 gactatcttt tatactttcg tgagtttttt taatcataat acaccgaaag taaggcagtt
     2461 gtaggcaatc aattattagc ttttgagcgg taaaatgcga cttgccacta tcacgtccac
     2521 cataaagaaa ttcaatatca atatcatttg gctctaaaat atgcctgtaa gtgtcaagaa
     2581 tagtactact atctattcct aagtttaact ttttcttttg caaaaccatt aatcaacacg
     2641 tttaccatca attatagtta taacaacttc atcaggaata aagtttttat tcgttaattc
     2701 tacttgcgta gttgcatact cgccaagcct tttacttaat tcaattctta aatcattgat
     2761 agttttttga attaaaagaa caatctgaat aggcagactt tccgttgcag atactttttg
     2821 ccctaagata aaagtcgtct ttattttacc tgataaaaca tcttgataat actcaatgtc
     2881 attttgcaat tccaaatctc tttcaaattt tgattttaag cctttttgag caaccttttt
     2941 tacttcttgc tcccttacct cctcgattgc tttttgttgg cttaaaagtt ctttttgata
     3001 cgtttcttta gcttctttta agtgattgta gaatgtagtt tcgggcaact cccacttact
     3061 actcattact cccaagatgt ccaatgtcgc atatccttcc ctcaacagct gtaaaatctg
     3121 ctt
//
LOCUS       DAOQCW010000162         3017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_18800_length_3017_cov_9.061783, whole genome shotgun sequence.
ACCESSION   DAOQCW010000162 DAOQCW010000000
VERSION     DAOQCW010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18800_length_3017_cov_9.061783"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="PLS16_10830"
     CDS             <1..1061
                     /locus_tag="PLS16_10830"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HNJ61621.1"
                     /translation="AKNTLIITKKKAISDIEKQYQDFGFTFNLWVINYESLHKIDNDF
                     DFVIIDEAHSISAYPKPSLRTKLIKEKFSNKRILLMSGTPAIESYSQWFHQFWISVFT
                     PFSQHKNFYRWADIFVNKKEKKIGTHSIIDYSDARTELIDNIIKPYIVKMTQAEAGID
                     IQIKEVFLHVKTPDSIQAFATKLLNNKAIKLHESNKYIMADTPTKMQTKIHQILNGTC
                     ILEDEHGATEPIILSNFKVDFIKEKFKDKKIIVMYYYQSERKMLEEAGIDCIQQSSTE
                     GVNLSEYDAIVYLNFGFSGKNFVQSRDRLSSVTRTKENVIYFIFEEKGINEKIYKRVS
                     KKQDFNLIAFKKDYEAKR"
     gene            1045..1284
                     /locus_tag="PLS16_10835"
     CDS             1045..1284
                     /locus_tag="PLS16_10835"
                     /inference="COORDINATES: protein motif:HMM:NF020354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HNJ61622.1"
                     /translation="MKQKDKLVKYWKDKGYFVINLIRVTPIGMPDYIALKPNEVIFIE
                     SKETWDRLSELQKIRLEMLNKIGFTCYVNFDKYIK"
     gene            1355..1933
                     /locus_tag="PLS16_10840"
     CDS             1355..1933
                     /locus_tag="PLS16_10840"
                     /inference="COORDINATES: protein motif:HMM:NF016024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rad52/Rad22 family DNA repair protein"
                     /protein_id="HNJ61623.1"
                     /translation="MEKVTKEMREALRMPLPKEAVKQHPTKTFLSSIKAIYVTERLND
                     VFGVGSWQIKVNHVTTTDKSMVVVKVEFSIPEYGIYFECYGGNDNGGENSKNFDLGDA
                     YKGATTDALTKIGSYLEIGIDVFKGLNTAPAPQQQFKKWLNITDSNGSDTKEWLNVIT
                     AINEKKITSIAEVKKYYNLSKEVEQKINELWK"
     gene            1924..2373
                     /locus_tag="PLS16_10845"
     CDS             1924..2373
                     /locus_tag="PLS16_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61624.1"
                     /translation="MEIIKKIETTMPVKANIDILANELTEGASSGFVNPLEFLVKIEF
                     LTKVLEQAKKQVKELALQNLTQPQELFGAKVEVADIGVKYDYSQNEIWQELKEKMQPL
                     EDELKKVEEQIKMATKIGKSIVDESTGELISPVQKTSTTSIKITLGR"
     gene            2405..2692
                     /locus_tag="PLS16_10850"
     CDS             2405..2692
                     /locus_tag="PLS16_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61625.1"
                     /translation="MRSYKNELEAIANDLLTQNAEAKGNENKPNYSNRDFMNATIIFQ
                     TALMDKMYDNQDYDGMSMDDRMKMAESCGLALRKLIHTYTGLDTHKIEEFL"
     gene            2763..3008
                     /locus_tag="PLS16_10855"
     CDS             2763..3008
                     /locus_tag="PLS16_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61626.1"
                     /translation="MGYSIRIPNGIISMVDVDFKCPKCECPHSEEDYYDRLYKSKNGL
                     IYKQCKGCKTKLGITTDIRGDVRVWLKEYERKDGLIA"
BASE COUNT         1225 a          397 c          556 g          839 t
ORIGIN      
        1 gggcaaaaaa tactttaata ataactaaaa aaaaggctat ttcggatatt gaaaagcagt
       61 atcaagattt tggctttaca tttaatctat gggttattaa ttatgaaagt ttacataaga
      121 ttgacaatga ttttgacttt gtaataattg atgaagctca ctctatttcg gcttatccca
      181 aaccgagcct tagaactaaa ttaattaaag agaaatttag caacaaacgc atcttattaa
      241 tgagtggtac tccagcaatt gaaagctact cacaatggtt tcatcaattt tggatttccg
      301 ttttcacgcc tttttctcaa cataaaaatt tctatcgttg ggcagatatt tttgtaaata
      361 aaaaagaaaa gaaaataggc acacattcga ttatagacta ttccgatgca agaactgaat
      421 taattgataa tataattaag ccttatatcg tgaagatgac acaggctgag gctggtattg
      481 acatacaaat aaaagaagtt tttttacacg tgaaaacgcc tgatagtatt caagcttttg
      541 cgacaaaatt acttaataat aaagctataa aattgcacga aagcaataaa tacataatgg
      601 cggacacacc gactaaaatg caaacaaaaa tccaccaaat actaaacggc acttgcattt
      661 tagaagatga acacggagca actgagccta taattctaag caattttaag gtagatttca
      721 taaaagaaaa atttaaggat aaaaagataa tagtaatgta ttactatcaa agtgaacgta
      781 agatgttaga agaagctggt attgattgta ttcagcagtc ctccacagag ggggtaaatc
      841 tttctgaata tgatgctatt gtttatttga atttcggctt tagcggaaag aattttgtgc
      901 aaagtagaga tcggctttcg tctgttactc gaactaaaga aaatgttatt tattttattt
      961 ttgaggaaaa aggcataaat gaaaaaatct acaaaagagt atcaaaaaaa caagatttta
     1021 atttaatcgc attcaaaaaa gattatgaag caaaaagata agttagtaaa atattggaaa
     1081 gataaaggct attttgttat taatttaata agagtaacac caataggaat gcctgattat
     1141 attgcactaa agccaaatga agtaatattt attgaaagta aggaaacgtg ggacagacta
     1201 tctgaattgc aaaaaatacg tttagaaatg ttaaataaaa taggttttac ttgctatgtt
     1261 aattttgata agtatattaa ataattatta aggattaaaa aagtacttga taattcaaaa
     1321 ctaaataact aattttacaa aaaaattaat cacaatggaa aaggtaacaa aagaaatgcg
     1381 agaggcacta agaatgccac taccgaaaga agcggtaaaa cagcacccca ccaagacttt
     1441 tttaagttca attaaggcta tatatgtaac tgaacgactg aatgatgtgt ttggcgttgg
     1501 aagttggcag ataaaagtta atcacgtaac aactacagat aagtctatgg tcgttgttaa
     1561 ggtagaattt tcgatacctg aatatggaat ttactttgaa tgttacggtg gtaatgataa
     1621 tggaggggaa aattctaaaa acttcgattt aggcgatgcc tataagggtg ctacaactga
     1681 tgcactaaca aaaataggct cgtatcttga aataggaatc gatgtattta agggattgaa
     1741 tacagctcca gcaccacagc aacaatttaa aaaatggcta aatataaccg atagcaatgg
     1801 aagtgacact aaagaatggt taaatgtcat taccgctata aatgagaaaa aaataacatc
     1861 tattgcagag gtaaaaaaat attataattt gtcaaaagaa gtggaacaaa aaataaatga
     1921 attatggaaa taataaaaaa aatagaaacg acaatgccag ttaaggctaa tatagatatc
     1981 cttgctaatg aacttacaga gggagcatcg agtggttttg ttaatccttt agaatttctt
     2041 gtcaaaatag aatttctaac caaggtcctt gaacaagcaa aaaagcaagt taaagaattg
     2101 gcattgcaaa acttaacgca accgcaagag ttatttggtg ctaaggttga ggttgctgat
     2161 ataggtgtaa aatatgacta ctcacaaaat gaaatatggc aagaattaaa ggaaaaaatg
     2221 caacctttgg aagatgaatt aaaaaaggtg gaggaacaaa taaaaatggc aacaaaaata
     2281 gggaaatcca ttgtagatga aagtacagga gagttaattt cgccagtgca aaaaacatca
     2341 actacttcta taaaaataac attgggaaga taataaaaaa aacagttaat taaaattaca
     2401 aaagatgaga agttacaaaa atgagttaga agcaatcgct aatgacttac taacacaaaa
     2461 tgcggaagca aagggaaacg aaaacaaacc caattattct aaccgtgatt ttatgaacgc
     2521 tacaataatt tttcaaactg ctttgatgga taaaatgtat gacaatcaag attatgatgg
     2581 aatgtcgatg gatgacagaa tgaaaatggc agaaagttgt ggacttgcat taagaaagct
     2641 gatacacact tatacaggtt tagacactca caagattgag gagtttcttt aaggttgccg
     2701 ctaacgccaa acgcatgtta tgcgttcggg cagatttgaa aactaattaa aaatataaaa
     2761 aaatgggata ctcaattaga ataccaaatg gaattatatc aatggttgat gtagatttca
     2821 aatgtccgaa atgtgaatgc ccacactcgg aagaagatta ttatgatagg ctttacaaga
     2881 gcaagaacgg tttgatttac aagcaatgta aaggttgtaa aactaaatta ggaattacaa
     2941 cagatataag aggtgatgtt cgtgtttggc tgaaagaata tgaacgtaag gacggtctga
     3001 tagcctgacg cataacg
//
LOCUS       DAOQCW010000163         2953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_19498_length_2953_cov_4.953416, whole genome shotgun sequence.
ACCESSION   DAOQCW010000163 DAOQCW010000000
VERSION     DAOQCW010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19498_length_2953_cov_4.953416"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..990
                     /locus_tag="PLS16_10860"
     CDS             136..990
                     /locus_tag="PLS16_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61627.1"
                     /translation="MNIFNSLIITCCFVFFIACGNGIDSEKYEGTLTIYADINQEKFL
                     NQFIPIFENIHPKSKLKFEYLDAQRLMQLYFTDSISNMILSRDLTSTELIYANQHRKL
                     VSKQYYFADDAIAIIGNKSNIDSIFDFKENKKTIVISKYNKDYLKFFTDSLLSRNIYA
                     LETPGNVIDYISKNDAIGLVPFSIFSNDKSIKHQELRTKIKLLKYQRNDSVFPLAQAY
                     ISDFSYPLVTHTVLITPKHPEPFLKLFSLFLFKEGTSRAILRYGLVPARLPERKVQLK
                     SESFVIEK"
     gene            1080..2339
                     /locus_tag="PLS16_10865"
     CDS             1080..2339
                     /locus_tag="PLS16_10865"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HNJ61628.1"
                     /translation="MSEENLTITLGGKTTVCPIIVGSENEPAIDIINLRQDTGFVTLD
                     SGYKNTGATTSKITFLDGEKGVLRYRGYDIADLAENSNFVEVIYALLNGDLPNVAQLA
                     EFEQQLKDNSAVPTEIGAIIKAMPKSAHPMATLAAATIALSGEFGADAAKATPQTIIN
                     ILAKFPVVVAMVIRNSQGLDFVPSDSKLSYVENFLNMTFGKVPSPVVVEAMDKLLILH
                     ADHEQNCSTSTVRMVGSSNANIYASICGGLAALWGPLHGGANQAVLEQLEAIEKDGGD
                     TEKFVDMAKDKESGFRLMGFGHRVYKNFDPRAKIIKVAADNVLTELNIQDRKLTIAKE
                     LEHAALTDEYFIKRNLYPNVDFYSGIIYQALGFPTDVFTCLFALGRIPGWVAQWKEMK
                     ENKEPIGRPRQIYTGATLRSYTNIDAR"
     gene            2462..2932
                     /locus_tag="PLS16_10870"
     CDS             2462..2932
                     /locus_tag="PLS16_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61629.1"
                     /translation="MKTFKLFAIITAIACITFSCKKDDNKIDDGGGGGGSKISSLVPK
                     GTIVPLAQRHITLTGIDEGASLRIEKGGKWWKIKDGAKIVSFNIVCSGQKVEDNVKTE
                     ELDEDEGPYISFYNNGDIMKSFSSDGGNAVKEGTWRWKDANKDAIRPLAKFTLS"
BASE COUNT         1047 a          389 c          534 g          983 t
ORIGIN      
        1 ttccattttc ttttgaattt ttaaccagca caacaaaagt agcacttgca gtgttactta
       61 tcctatatgg tatttttagg atgtatcgtg ctgtaaaaat ggcaaatatt tctaatgaaa
      121 tttaattgtt ttagaatgaa tatatttaat agtctaatta ttacgtgttg ttttgtcttt
      181 tttattgctt gtggcaatgg aattgactcc gaaaagtatg aaggaacatt aacaatttat
      241 gcagacatca accaagaaaa atttttaaat cagtttattc ctatttttga aaatatccat
      301 ccaaagtcta aattaaaatt tgaatacctt gatgctcaac gcctgatgca attatatttt
      361 acagatagta tttctaatat gattttgagt agagatttaa cttcaacgga attaatctat
      421 gcaaatcaac ataggaaatt agtttcaaag caatattatt ttgctgatga tgcgatagca
      481 ataattggta ataaatctaa tatagattcc atttttgatt ttaaagagaa taaaaaaact
      541 attgttatta gtaaatacaa taaggattat ctgaaatttt tcacggattc tttgttatct
      601 agaaatattt atgcgttaga aactccagga aatgtgatag attatatttc gaaaaacgat
      661 gcaattggtt tagtgccatt tagtatattt tctaatgata aatccattaa acatcaagaa
      721 ctaagaacta agattaaact gttgaaatat caaagaaatg attctgtttt tcctttagct
      781 caagcctata tttctgattt tagttatcct ttggtaacac atacagttct aataacacca
      841 aaacatcctg aaccattttt gaaactgttc tctctatttc tttttaaaga gggaacttca
      901 agagccattt tacgatatgg tttagttcct gctagattac cagagcgaaa agtgcaacta
      961 aaaagcgaaa gttttgttat agaaaagtaa gaatacttat agttttaagc aagtaacaat
     1021 atcttaattt cataagtgta actatttttt ttaattttat caaatataat taaattgcta
     1081 tgtcagaaga aaatttaacc attaccttag gcgggaaaac tacagtatgt ccaattattg
     1141 ttggaagtga aaatgaacct gctattgata ttataaactt gagacaagat acaggttttg
     1201 taaccttaga ctctgggtat aaaaatactg gagcgactac aagtaaaatt actttcttag
     1261 atggagaaaa gggagtttta agatatagag gctatgatat tgctgattta gctgaaaact
     1321 ctaattttgt tgaggtaata tatgcattat taaatggaga tttgccaaat gttgcccaac
     1381 tagcagaatt tgaacaacaa ttaaaagata attcagctgt acctactgaa ataggtgcaa
     1441 ttattaaggc aatgccaaaa tcagctcatc caatggcaac attggcggca gcaacaattg
     1501 ctttatctgg tgagtttgga gcggatgcag caaaagctac acctcaaaca attataaata
     1561 ttttagcaaa attccctgtt gtagttgcta tggtaattag aaactctcaa ggcttagatt
     1621 ttgttccaag tgatagtaag ctttcttatg ttgagaattt tttaaatatg acattcggga
     1681 aagttccatc gccagtggtt gttgaggcta tggataaatt attaatctta catgctgatc
     1741 acgaacaaaa ctgctcgaca tctactgtaa gaatggtagg ttcatctaat gcaaatatat
     1801 atgcttcaat ttgtggtgga ttagctgctc tttggggtcc attacacggt ggagctaatc
     1861 aagctgtttt ggaacaatta gaagcaatcg aaaaagatgg tggagatact gagaaatttg
     1921 ttgatatggc aaaggataag gagtctggat ttagattaat gggctttgga catagagtgt
     1981 ataaaaactt tgacccaaga gctaaaatta ttaaagttgc tgcagataat gtattgacag
     2041 agttaaatat tcaagataga aaattgacta ttgctaaaga attagagcat gctgcattga
     2101 cagatgaata ttttatcaaa cgtaatttat atccaaacgt agatttttat tctggcatca
     2161 tttatcaagc attaggattc ccaactgatg tatttacatg cttatttgct ttaggaagaa
     2221 ttccaggatg ggttgctcaa tggaaagaaa tgaaagaaaa caaggaaccg ataggacgac
     2281 caagacaaat ctatacaggt gcaactttaa gaagttatac taatattgat gcaagataat
     2341 agtgtttaat aatggataag tgaggcggaa tttttatatt ccgccttttt tgttaaaaga
     2401 tattaaaaag aaagctaggt atatatttta tattagatta gtgtaactaa atattttaat
     2461 tatgaaaaca ttcaaattgt ttgcaatcat tacagcgatt gcatgtatta cttttagctg
     2521 taaaaaagac gataacaaaa ttgacgatgg tggtggagga ggtggttcta aaattagttc
     2581 tcttgttcct aagggaacca ttgttccttt agctcaacga catattacat taactggcat
     2641 cgatgaaggt gcttcgctca gaattgaaaa aggaggaaag tggtggaaaa ttaaagatgg
     2701 tgcaaagatt gtatctttta atatcgtttg ttctggtcag aaagttgagg acaatgtaaa
     2761 gactgaagaa cttgatgaag atgaaggacc ttatatttct ttttataaca atggagacat
     2821 aatgaagagt ttttcttcag atggtggaaa tgcagtgaaa gaaggaacct ggagatggaa
     2881 agatgcaaat aaagatgcta ttagacctct tgcgaaattc acactaagct aataaaaaac
     2941 tatttccaat cta
//
LOCUS       DAOQCW010000164         2612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_24353_length_2612_cov_4.832616, whole genome shotgun sequence.
ACCESSION   DAOQCW010000164 DAOQCW010000000
VERSION     DAOQCW010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24353_length_2612_cov_4.832616"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PLS16_10875"
     CDS             <1..296
                     /locus_tag="PLS16_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016284764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HNJ61630.1"
                     /translation="DKPGPTIVGRGGGTGGPVIAVHPNCKRRFTVRETARIQSFPDNF
                     EFYGSVSSQFRQIGNAVAVEFAKHLGKVLKDFEDGKLKNKKATSNVQAELLLV"
     gene            complement(293..1291)
                     /locus_tag="PLS16_10880"
     CDS             complement(293..1291)
                     /locus_tag="PLS16_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61631.1"
                     /translation="MDSILKFPVIPKAIYIEYRKLKKTPVSNHETFKKLITGLVRDAK
                     SNGTMTSKLAKLILFDWKSYINHPAIYKEKSTASALENRIALLGHGNTSDVLPKKDPL
                     ISTLLSKNEYDKLPIEVQSKICSNFREKGDVIFYDSKLNSSYKVSIKSLIFDNKEINF
                     GALEFASIIRGILPDEFLALGERRSKINKTIGKRDYEIGRGSRQQLGEFFDYIKADGK
                     WKIFLERWKIVFSGIFKEDVIIYIKEHEKLKLYLISNKSFVECIYNSLVDTKDGYTNM
                     IINRWEGNSIRVDRDLLIKHVDFKVEESFDNFFDENSIKIKMQEMENLKYKKLLQD"
     gene            complement(1294..1500)
                     /locus_tag="PLS16_10885"
     CDS             complement(1294..1500)
                     /locus_tag="PLS16_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNJ61632.1"
                     /translation="MDIKKKFGEKVKLLRTKKGWSQEKLALSADLDRTYIPSIEKGER
                     NVSISVIEKIAKAFKVSISELFKD"
     gene            1765..>2612
                     /locus_tag="PLS16_10890"
     CDS             1765..>2612
                     /locus_tag="PLS16_10890"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HNJ61633.1"
                     /translation="MSIRKTTVLFCLFVLHFISIAQTQYKPKSPSPFQVLYDLENEKL
                     EYISKKLDSLTTHTVPKDAILIKHKGFYAAYSSTHKQPYWVSHIVPKDVLYGSYTRND
                     GFILDSLYLNTADSTDFWGSGYDRGHLAPAADFRWNKEAMRESFYYTNIATQNKDFNR
                     GAWAKLETQVREFAIDADEIYVVTGVVLNQKLAQIPQGTYSVSIPKYFYKILYDLKQP
                     KAIAFLMPNKNIIYDIDKYIVSVDSIESLTELDFLPILEDKIESKIEKEKDIYAWNPN
                     HQKYTET"
BASE COUNT          908 a          433 c          357 g          914 t
ORIGIN      
        1 gggacaaacc tggaccgaca atagtaggaa gaggcggagg aacaggcgga cctgttattg
       61 cagtacatcc taattgcaaa agaagattta cggtacgaga aaccgctaga attcaatctt
      121 tccctgataa ttttgaattt tatggttcag tgtcttcaca atttcgacaa atcggaaatg
      181 ctgttgcagt tgaatttgca aaacacttag gaaaagtttt aaaggacttt gaagacggta
      241 aattgaaaaa taaaaaagca acaagtaatg ttcaagccga attattattg gtttagtctt
      301 gtaaaagctt tttatatttt aaattttcca tttcttgcat ttttattttg atgctatttt
      361 catcaaaaaa attatcaaat gactcttcaa ctttgaagtc aacatgtttt attaataaat
      421 ctctatcgac tcttatcgaa tttccttccc atctatttat tatcatattc gtgtagccat
      481 cttttgtatc gaccaaacta ttgtaaatac attcaacaaa agacttgtta ctgataagat
      541 acaatttcaa tttttcgtgt tctttaatat aaataattac atcttcctta aaaattccag
      601 aaaaaacaat tttccaccgt tctaaaaata ttttccattt cccatctgct tttatataat
      661 caaaaaactc tccaagttgt tgtctagacc ctcttccaat ttcatagtct cttttaccaa
      721 tagttttgtt aatcttactt ctcctttctc cgagtgccaa aaactcatca ggcagaatac
      781 ctctaataat agatgcaaac tctaatgctc cgaaattaat ttctttattg tcaaatatta
      841 aagacttaat agaaacctta taacttgaat taagtttaga atcatagaaa attacatcgc
      901 ccttttctct gaaatttgag caaatttttg attgaacttc tattggcaat ttatcgtatt
      961 catttttact taacagagta gaaattaaag gatccttttt gggcaataca tctgaagtat
     1021 ttccgtgacc taaaagtgct atcctattct ctaaagcact agccgtagat ttttctttat
     1081 aaatcgcagg atgattaata tagcttttcc aatcaaataa aattaacttg gcaagtttag
     1141 atgtcattgt tccatttgac ttagcatccc taaccaatcc agtaattaat tttttaaatg
     1201 tttcatggtt agaaacaggc gttttcttta actttcgata ctcaatgtaa atagcctttg
     1261 gtataactgg aaatttaagt atactatcca taatcaatct ttaaataatt cactaatact
     1321 tactttaaat gcttttgcga ttttttcaat cacagaaata gaaacatttc tttccccttt
     1381 ttctatactt ggaatatatg tacggtctaa atctgctgac aatgctaact tttcttgtga
     1441 ccaacctttt ttagttcgta acaacttaac cttttcccca aattttttct taatatccat
     1501 tgtgtaaaga ttgtgatatg tagacaatta tacaacggat gattgtctac ataaagtaca
     1561 aaatattaaa aaaataaaac tctataatca aacgcctaac aaaatcgaac ctcaatactg
     1621 gctagttcgt agttcgaaaa atagttttga ataaatttga atgtcaggac tcagaataaa
     1681 agtttaaata taaattcgtt accttaagga aggctaaaat tatcctcaaa attagattgg
     1741 aataaatttt gtacttttat aattatgtct attagaaaaa ctactgttct attttgctta
     1801 tttgtgttgc attttatttc aattgctcaa actcaatata aaccaaaatc tcctagtccc
     1861 tttcaagttt tatatgattt agaaaatgaa aaattagagt acatctctaa aaaactagat
     1921 tccttaacaa cgcacacagt accaaaagat gccattctta ttaaacataa aggtttttat
     1981 gccgcatatt ccagtacaca taagcaacct tattgggttt cacatattgt gcctaaagat
     2041 gtattatacg gaagttatac ccgaaacgat ggttttattt tagatagtct gtatctaaat
     2101 actgctgata gtacagactt ttggggaagt ggttacgacc gtggacattt agctcctgcc
     2161 gcagattttc gatggaataa agaagccatg cgagagtcct tttattatac caatattgca
     2221 acacagaaca aagatttcaa tcgtggagca tgggcaaaat tggaaactca agtaagagaa
     2281 tttgccattg atgctgatga aatttatgtc gtaactggtg ttgtattgaa tcaaaagtta
     2341 gcacaaatac ctcaaggaac atatagtgtt tccattccaa aatatttcta taaaatactt
     2401 tatgatttaa aacaacctaa agctatagca ttcttgatgc ccaacaaaaa tatcatttat
     2461 gatatagata aatatattgt ttctgttgat agtattgaaa gtttaactga gctagatttc
     2521 ttgccaatat tagaagataa aattgaatct aaaattgaaa aagaaaaaga tatttatgct
     2581 tggaatccaa atcatcagaa atatacagaa ac
//
LOCUS       DAOQCW010000165         2592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_24740_length_2592_cov_5.187623, whole genome shotgun sequence.
ACCESSION   DAOQCW010000165 DAOQCW010000000
VERSION     DAOQCW010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24740_length_2592_cov_5.187623"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PLS16_10895"
     CDS             <1..323
                     /locus_tag="PLS16_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61634.1"
                     /translation="IKQDMPNSISKMEQQTLYKVQGRDGISYENATLGNSIPNKSVDI
                     SASQMSDKLIQAGGEKTLINGGYRIDLNGTRYVFYNRGSTGSAGVSIQINGLTIQKYL
                     LNGW"
     gene            328..906
                     /locus_tag="PLS16_10900"
     CDS             328..906
                     /locus_tag="PLS16_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61635.1"
                     /translation="MEIINFNSPIFNITNKIEHEIFIFEKKFPDFLFKIPFEKYYFLW
                     DSMSFNTLKLFQEVVLPLLNNGLCKEAILTSLLPHSNGKELLKFKLDNSDSAEIFNQT
                     MRYDPISTNESHNIMYSTQAPAIYSMDKSWAIVGDSWDTDITVLAINSELKQFLPIND
                     FCTIETIEKLLFFVQDENVKTNLLKQIKKNWT"
     gene            924..1610
                     /locus_tag="PLS16_10905"
     CDS             924..1610
                     /locus_tag="PLS16_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61636.1"
                     /translation="MKKNCNLRLFPLVLVLLFSDSCVSTKRYTSFTKEKVWELQKTSQ
                     VTQKLDYLEIRTDSLIVQDSLIKVQKLKSFFIPAIFYWQSEHSLKCELSPKVPVRFFE
                     QEFMRLADSADLKSKLNGQKIELSILQIPNSFVYTHKEKTIIFIVAYIVSSLEAIYPD
                     NKELKIRYRIVENDENKKTGIITIPNTELPIKNVWKSSKKFTWVYLDKYKKINEKTAK
                     DVFENLIILF"
     gene            1792..2085
                     /locus_tag="PLS16_10910"
     CDS             1792..2085
                     /locus_tag="PLS16_10910"
                     /inference="COORDINATES: protein motif:HMM:NF013500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HNJ61637.1"
                     /translation="MKKIKEVIIPKEIKTKDELLKHLSFVLNLPKYFGFNWDALQECL
                     CDLHWQKEKKLIIIHEQGFNLKETDNDIYRDVLKASVESWHSSDIHTIEVYFQ"
     gene            2146..2466
                     /locus_tag="PLS16_10915"
     CDS             2146..2466
                     /locus_tag="PLS16_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61638.1"
                     /translation="MRKLHTKEKKLILCLLKKKDTSFNCPNLNEVYVEDMDDGGMGSL
                     YIISEIKEKKNRKMLKSIAELTVKDKDGITISITLNIDHEGKLFELDIWKVDFSPLID
                     YPEC"
BASE COUNT          990 a          337 c          433 g          832 t
ORIGIN      
        1 ttattaaaca ggatatgcca aatagcatat ctaagatgga gcagcaaact ttgtacaaag
       61 ttcagggaag ggatggaatt agttatgaaa acgctacttt gggtaattca attcctaata
      121 aaagtgttga tatttctgcc tctcaaatgt ctgataaact tatacaagca ggtggtgaaa
      181 agacacttat caatggaggc tatagaattg acttaaatgg aacaagatat gttttttaca
      241 acagaggttc tactggatct gctggagttt ctattcaaat caatggatta actatacaaa
      301 aatatttatt aaacggttgg taatgctatg gaaataataa attttaattc gccaattttt
      361 aatattacaa ataaaatcga acatgagatt tttatttttg aaaaaaaatt ccctgatttc
      421 ttatttaaga taccatttga gaaatattat tttttatggg attcgatgtc ttttaacaca
      481 ttaaaattgt ttcaagaagt tgtattgcca ctattgaaca atggcttatg taaagaagct
      541 attcttacat cgttactacc acattctaat ggcaaggaat tattaaagtt taaactcgac
      601 aattctgact ccgctgaaat atttaatcaa acaatgcgct acgacccgat tagtacgaat
      661 gaatcacata atataatgta ttcgactcaa gcacctgcta tttatagtat ggataaatca
      721 tgggctattg taggcgattc atgggatact gatattacag ttttagcgat aaacagtgaa
      781 cttaaacaat ttcttccaat taatgatttt tgtactattg aaacaattga aaaactatta
      841 ttttttgttc aggatgagaa tgtcaaaact aatttattga aacaaataaa aaagaattgg
      901 acataataga attaatttga aaaatgaaaa aaaattgtaa tttaagatta ttcccccttg
      961 ttctggtact tttattttca gatagttgtg taagtacgaa acgttatact tcatttacaa
     1021 aagaaaaagt atgggaattg caaaaaacca gtcaagtaac acagaagctg gattatcttg
     1081 aaataagaac agacagcttg attgttcagg acagtttgat aaaggttcag aaattaaaat
     1141 ctttttttat tcccgcaata ttttactggc aatcggaaca tagtctaaaa tgcgaactta
     1201 gtcctaaagt tcccgtaagg ttttttgagc aggaatttat gcggcttgcc gattcggctg
     1261 atttaaaatc gaaattaaac ggtcaaaaga ttgaattatc aatcttacaa attcctaaca
     1321 gttttgttta tactcataaa gaaaaaacta tcatttttat tgttgcttat attgtcagta
     1381 gcttagaagc aatataccct gataacaaag agctaaaaat aagataccga atagtagaaa
     1441 atgacgaaaa taaaaagacc ggaattataa ccatacctaa tacggaactg ccgataaaaa
     1501 atgtatggaa atcatcaaaa aagtttacgt gggtttatct tgacaagtat aaaaaaatta
     1561 atgagaaaac agcaaaagat gtttttgaaa atttgataat attgttttga gaatttatag
     1621 ctactactac attacaaaat atcacctgat tctaattgct aaaagtgcac acgcaggaca
     1681 tcccaacttc gggaattagt ggacctggtg cgcaaatgat agtaacaggc gccaatgggg
     1741 aagtatggtt tagcccagac cattatagaa catttataaa aatcagataa tatgaagaag
     1801 attaaagaag tcataattcc aaaagaaatt aagacaaaag atgagctttt aaagcactta
     1861 tcatttgtct taaatttacc taagtatttt ggctttaatt gggatgcatt gcaagaatgt
     1921 ttgtgtgatt tacattggca aaaagaaaaa aaattaataa ttattcatga gcaaggattt
     1981 aatcttaaag aaactgataa tgatatttat agagatgttt taaaagcatc tgttgagagt
     2041 tggcatagtt cagatataca tacaatagaa gtttattttc aataactata tatgaattac
     2101 cttcaggggc aatcaatatt ggaagaattg ttattcatta gtaaaatgag aaagttacat
     2161 acaaaagaaa aaaagctgat tctttgcttg ctaaaaaaga aagatacatc atttaattgt
     2221 cccaatctaa acgaagttta tgttgaagat atggatgatg gaggcatggg aagtctttat
     2281 attatttcag aaattaagga gaaaaagaat agaaaaatgt taaagagcat tgctgagttg
     2341 acagttaaag acaaagatgg aataacaatt agtattactt taaatattga tcatgaaggt
     2401 aaactttttg aattagatat atggaaagtt gattttagtc ccttaattga ttaccctgaa
     2461 tgttaaggtg gtaatggaat tccattttgc tcctgcacca gttttaacac ctaacagagt
     2521 taagccatga atccatatta ttatctgttt tacataattt acaagtttgt taaacttaca
     2581 acaaaggaag aa
//
LOCUS       DAOQCW010000166         2539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_25705_length_2539_cov_6.087359, whole genome shotgun sequence.
ACCESSION   DAOQCW010000166 DAOQCW010000000
VERSION     DAOQCW010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25705_length_2539_cov_6.087359"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="PLS16_10920"
     CDS             <1..418
                     /locus_tag="PLS16_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61639.1"
                     /translation="QKDTTAKTIKTPKVKPNKNTIKIEKLKVVIQKDSTSKVIKMPKV
                     KADKIPALIYKIKLATVLVDPGAKNFENISSFGQITFTKIANQTIYYIGNYKSYLEAV
                     KYLQKIITQGYSKAYITPFYLDKEISISEAIEKENK"
     gene            complement(428..1240)
                     /locus_tag="PLS16_10925"
     CDS             complement(428..1240)
                     /locus_tag="PLS16_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61640.1"
                     /translation="MEDTIKVYGDTMVNSQTEENRFIASYRIVKNLVSILKIKNSYQY
                     QLDSLLPMQILNSPDNKFRIFTWFVINDNQQYRYFGAIQMNSDTLSLFPLVDYSEFIE
                     KPETQTVDNNNWYGALYYQIVPVKQKKKTYYMLLGSNGFTLVSNKKVMDVLWFSEDNK
                     PRFGAPLIEKDNKTLNRFILEFSNDTWAALRYVPEETKIIYDHVLPNDEDLVGFFSQY
                     LPDGTYEGFHWKKGKWHHIDMIEYQKREDGEAPNVQKKEKIDYKNLYKKKVQ"
     gene            complement(1440..>2539)
                     /locus_tag="PLS16_10930"
     CDS             complement(1440..>2539)
                     /locus_tag="PLS16_10930"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNJ61641.1"
                     /translation="GNIFALYAQLDKKFKDRVTLSGGLRMETYKYEKNVGVTPPVGRL
                     GMNIRVGKESFLRFNLGQAFRFPSFAERFVDETVGNESKTYHLVDTTYPGDGLGYVRE
                     RDSVVSNPLLKIMPNPNLKPEYGWTSEIGFQQIFKTKSNKYSGVFDAAFYWQEYRDLV
                     DFGGVIDSSSNAKINLKAQNISRARIAGWDISFKNNMNFKNHSFDLNLGYTYAFPIEL
                     NSNIQNPNFDNSTIGGYIKNMFKSFTKRIGGIDFYQVLKYRNRHLITLDAEYSFKKKI
                     TIGLNARYYSNFEGYDIVFLGIEGIPEKVSQIDKKGKGDWVLNGQLFYTVKEKHNFGF
                     IIKNMLNKEYWLRAGKLESPMNFTVQYRIEF"
BASE COUNT          903 a          424 c          297 g          915 t
ORIGIN      
        1 gcaaaaggat actacagcta agacaattaa aacgcctaaa gtcaagccta ataagaatac
       61 tataaagatt gaaaaactta aagtagtgat tcaaaaagat tctacttcta aagttattaa
      121 aatgccgaaa gtaaaggcag ataaaattcc agctttaatt tacaaaatta aattagcaac
      181 tgttttagta gatcctggag ctaaaaactt tgaaaatatt tcttcatttg gccaaattac
      241 ttttactaaa atagccaatc aaactatcta ttatattgga aattataaaa gttatttgga
      301 agctgtaaaa tacctacaaa aaattattac tcaaggatat tccaaagcgt atatcacacc
      361 attttacctt gataaagaaa tctctataag tgaggcaatt gaaaaagaaa ataaataagt
      421 aaacaaacta ttgcactttt ttcttatata aatttttata gtctattttt tcctttttct
      481 gtacattagg agcttcacca tcttctcttt tttgatattc tatcatatca atatggtgcc
      541 atttaccttt tttccaatga aaaccttcat aagttccatc aggtaggtat tgcgagaaaa
      601 agccaactaa atcttcgtca ttcggcaaaa catggtcgta aataatctta gtttcttcag
      661 gaacatatcg caatgctgcc caagtatcat tactaaattc taaaataaat cgattgagtg
      721 ttttattgtc tttttcaatc aacggcgcac caaatctagg tttgttatct tcgctaaacc
      781 atagtacatc catcactttt ttattactaa ccagtgtaaa tccatttgaa cctaatagca
      841 tataataggt tttctttttt tgttttactg gcactatttg ataatacaat gctccatacc
      901 aattattatt atcgacagtt tgagtttctg gtttttcaat aaattctgaa taatcaacta
      961 aagggaaaag tgaaagtgta tccgagttca tctgaattgc accaaaatat cgatattgtt
     1021 ggttatcatt aatgacaaac caagtaaaaa ttctaaactt attatctggt gagttaagga
     1081 tttgcatcgg caataaagaa tctaattgat attgataaga atttttaatt ttcaaaatac
     1141 ttacaagatt ttttacaatt ctatagctag caataaatct attttcttca gtttgactat
     1201 tgaccattgt atcgccatat actttaatcg tatcttccat tctttgaata aaaacagaat
     1261 ccttttttga aaattgggct tgggaatgaa aagaaattcc aataaataaa aaaagtaaaa
     1321 aaatgtattt ttgcataata aaatctgtaa tacaaaataa gcaccatttt tataaaaaat
     1381 ggtgcttatt caaagtttta aatataatta acgtaaataa gaataagtaa actaaacttt
     1441 tagaactcta ttctatattg cacagtaaaa ttcattgggg attctaattt acctgcacgc
     1501 aaccaatatt ctttattcaa catattttta atgataaatc cgaaattatg cttttctttt
     1561 acagtataaa ataattgacc attaagtacc caatcgcctt tccctttttt gtctatctga
     1621 ctaacttttt caggtattcc ttcaattcct aagaatacaa tatcatatcc ttcaaaatta
     1681 ctataataac gtgcattcaa accaattgta attttctttt taaaactata ttctgcatca
     1741 agtgtaatta agtggcgatt tctatatttt aatacctgat aaaaatctat acctccaatt
     1801 cttttagtaa atgacttaaa catatttttt atataaccac caattgtact attgtcaaaa
     1861 ttaggatttt gaatattcga gtttaattca ataggaaatg cataagtata acccaaattc
     1921 aaatcaaatg aatgattttt gaaattcata ttatttttaa aagaaatatc ccaacctgca
     1981 attcttgctc tactaatatt ttgtgccttt aaattaattt ttgcatttga gctggaatca
     2041 atcacacctc caaaatcaac taaatctcta tattcttgcc aataaaaagc agcatcaaat
     2101 acgcctgaat atttattgct tttagtttta aatatctgtt gaaatccaat ttcagaagtc
     2161 catccatatt ctggttttaa atttggattt ggcataattt ttaataatgg attagaaaca
     2221 acactatccc tttctcttac atatcccaag ccgtcccctg gataggtagt gtccaccaaa
     2281 tgataggttt tagattcatt tcctactgtt tcatctacaa atcgttctgc aaatgaagga
     2341 aaacgaaacg cttgacctaa attaaatctt agaaaacttt cttttccgac gcgtatattc
     2401 atgcccaatc ttcctactgg cggtgttact cctacattct tttcatactt atatgtttcc
     2461 attcttaaac ctccagataa ggtaactcta tctttaaatt ttttatctaa ttgggcatat
     2521 aatgcaaaaa tatttccaa
//
LOCUS       DAOQCW010000167         2528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_25932_length_2528_cov_5.602103, whole genome shotgun sequence.
ACCESSION   DAOQCW010000167 DAOQCW010000000
VERSION     DAOQCW010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25932_length_2528_cov_5.602103"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PLS16_10935"
     CDS             complement(<1..218)
                     /locus_tag="PLS16_10935"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNJ61642.1"
                     /translation="MKVLDKYIIKQFLITFFFAVFLLSLIAIIIDITEKIDNFLENNA
                     PLKTIIFDYYVNFVPWIVLLIAPLFVFIS"
     gene            complement(229..1356)
                     /gene="tgt"
                     /locus_tag="PLS16_10940"
     CDS             complement(229..1356)
                     /gene="tgt"
                     /locus_tag="PLS16_10940"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNJ61643.1"
                     /translation="MNFSIQYQDAQSNARVGKITTDHGTIETPIFMPVGTQGAVKAVH
                     FDELHNDIQAQIILGNTYHLYLRPGLEILEKAGGLHQFNTWKKPILTDSGGYQVYSLS
                     HRRKITEQGVTFQSHIDGSKHLFTPEGVMDIQCTIGADFMMAFDECTPYPCAYNYAKK
                     SLKLTHDWLDRCIERFDNTPAKYGYEQQLLPIVQGSVYKDLRIKSAEFIASKNRFGNA
                     IGGLAVGEPAEEMYAMTEVVTNILPKDKPRYLMGVGTPANILESIALGIDMFDCVMPT
                     RNARHGLLFTSQGIINIKNQKWKDDFSPIDSLTCPTSQVHSKAYLRHLFVTAEILAMQ
                     IASIQNLSFYLWLVNEAREQIKAGTFSNWKAPMIQKIMQRL"
     gene            complement(1420..2427)
                     /locus_tag="PLS16_10945"
     CDS             complement(1420..2427)
                     /locus_tag="PLS16_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61644.1"
                     /translation="MRTLKHKIFIKNNLSSVSFLFCFLLFSSCSGFKNSQHAIHLDKS
                     YCNKQKQTIYTQADLPKPLYELDIDTVLKNRFSNTSLNMANAIGILDILTDYTKLLLD
                     SSHSSSLEKRIAILEHIHQIDQKINFTSLEISAIASELDCEEERTNQFASYLKERESQ
                     TEKGLVIGSIIVGAAGAIAAEVVAKKSSNDNLVTGFTIGTSLVETSLGVLMLVNKRKI
                     DFKHSDNALTDIWNNSTVSSYFPPSIWYYLTYENPNTKEKSLAKLLVEKWFLFGQVEE
                     ESSNPKDNNNKLYFGNGGQYGADELDNRADMLDQTESYVTLMKQDLKTLMAEIAKLDK
                     K"
BASE COUNT          859 a          421 c          374 g          874 t
ORIGIN      
        1 gaaataaata caaaaagtgg agctatcaat aaaacaatcc acggtacaaa attcacataa
       61 taatcaaaaa tgatggtttt gagtggagca ttattttcta aaaaattatc aattttttca
      121 gttatatcaa ttataatagc aattaaactc aatagaaaaa cagcaaagaa aaaagtgatt
      181 aaaaactgct ttataatata tttgtctaat actttcaaga ctgttgattt ataatctttg
      241 catgattttt tgtatcatag gagctttcca attggagaaa gtaccggctt taatttgttc
      301 acgtgcttca ttaaccaacc aaagatagaa acttaaattt tgaattgatg caatttgcat
      361 cgctagaatc tcggctgtta caaataaatg acgtagatat gcttttgaat gtacttgact
      421 agttgggcaa gttaaggaat caatcggtga aaaatcatct ttccattttt ggttttttat
      481 attaataatt ccttgagagg taaataataa accgtgtcga gcatttcttg ttggcattac
      541 acaatcaaac atatcaatac ctaaagcaat ggattctaaa atatttgcag gcgtaccaac
      601 acccatcaaa tatcgtggtt tatcttttgg caaaatgttc gttacaactt cagtcattgc
      661 atacatctct tctgcaggtt cgccgacggc taaaccacct atggcattgc caaatctatt
      721 ttttgaagcg ataaattctg cagattttat tcttaaatct ttataaacac taccttgaac
      781 aatcggcaat aattgttgct catagccata ttttgcaggt gtattatcga atctttcaat
      841 acatctatcc aaccaatcgt gtgttaattt taaagatttt ttggcataat tataagcaca
      901 aggataaggt gtacattcat caaaagccat cataaaatct gcaccgatag tacattgtat
      961 atccattacg ccttctggag taaacaagtg tttgctacca tcaatatgcg actgaaaagt
     1021 aacaccttgt tcggtaattt ttcttctatg acttaatgaa taaacttgat aaccaccact
     1081 atccgttaat atcggctttt tccaagtatt gaattgatgc aaaccaccag ctttttctaa
     1141 tatttctaaa cctggacgta aatataaatg ataagtattt cctaaaataa tttgagcttg
     1201 gatatcatta tgcaattcgt cgaaatgcac agcttttacg gcaccttgag taccaactgg
     1261 cataaaaatt ggcgtttcta tggtgccgtg atcggttgta attttaccga cacgagcatt
     1321 cgattgagca tcttgatatt gtatagaaaa attcaaattt tttgaacaaa gataaaatta
     1381 aactcgtaat gttcaaatat agctaagaat ccaattggct tactttttat caagcttagc
     1441 aatttctgcc ataagcgttt ttaaatcttg tttcattaag gtaacataag attctgtttg
     1501 gtcgagcatg tcagcacgat tatctaattc atcagcacca tattgaccac cattcccaaa
     1561 atacaatttg ttgttattgt cttttggatt actgctttct tcctcaacct gtccaaataa
     1621 aaaccatttt tctactaata attttgctaa agatttctct ttcgtattag gattctcata
     1681 agtgaggtaa taccaaattg aaggtggaaa ataacttgaa accgttgaat tgttccaaat
     1741 atcagtcaaa gcattatctg aatgtttaaa atcaattttt cttttattca ctagcattaa
     1801 aacacccaat gatgtttcta ctaaactcgt tccgatagta aaaccagtta ccaaattatc
     1861 atttgatgat ttcttagcaa cgacctctgc cgcaattgca ccagcagcac caacaattat
     1921 ggaaccaata accaagcctt tctccgtttg gctttcacgt tcttttaaat aagatgcaaa
     1981 ctgattggtt cgctcctctt cacaatccaa ttcactagca atagcagaaa tttcgagaga
     2041 agtaaaattt attttttggt caatttggtg tatatgttct aaaatagcaa ttcttttttc
     2101 gagagaagac gaatggctac tatctaagag aagttttgtg tagtcggtta aaatatctaa
     2161 tattccgata gcatttgcca tattgagtga agtattagaa aatctatttt ttaaaacagt
     2221 atcaatgtct aattcataaa gaggtttagg taaatctgct tgggtataaa ttgtctgttt
     2281 ttgtttattg caataacttt tatctaaatg aatggcatgt tgagaatttt taaatccaga
     2341 gcaagaagag aataaaagaa aacaaaatag aaaggaaaca gagctaagat tatttttaat
     2401 aaatatttta tgctttagcg ttctcatttt gtatcaaaaa taataaaaat tagattatgt
     2461 atggtttaaa accttaactt atcaaaaatt tcatacgcaa taatctgact attttttatt
     2521 gactgctc
//
LOCUS       DAOQCW010000168         2497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_26565_length_2497_cov_4.035627, whole genome shotgun sequence.
ACCESSION   DAOQCW010000168 DAOQCW010000000
VERSION     DAOQCW010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26565_length_2497_cov_4.035627"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..1859)
                     /gene="ltrA"
                     /locus_tag="PLS16_10950"
     CDS             complement(159..1859)
                     /gene="ltrA"
                     /locus_tag="PLS16_10950"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HNJ61645.1"
                     /translation="MIRYEWNEIPWRKLEIKTFKLQKRIYRAMQQDNIKLVHKLQRLM
                     LASTSARILATRKVSQDNRGRKTAGIDGKIALTQRERMNLALSINLKGRSKPLRRIWI
                     AKSGTNEKRPLGIPTIEDRAKQALLKMALEPEWEAKFEPNSYGFRPGRSGHDAIVAIH
                     NAITKKTGYVLDADISGCFDNINHQVLLNKLATTPMKRRIIKGWLKAGVIDGEVFYTT
                     KNGTPQGGVISPLLANVALHGLEKDTKNSLAKDLLEDFKKKTGRSSNIKAQTAISIIR
                     YADDFVVIHENKEIIMKAKATIEKWLANIGLKLKESKTNIVHSLNSDNGQKPGFDFLG
                     YTIRQFPDKNKRRGYKSYTRPSKQGQKEHLLTIKKILRNMIAMPQEKVIEKLNPIIKG
                     WSNYYTPGVSSSIFRKMDHKLHKKLWQWSIRRHPDKSRAFVQFKYFRQHGNYKWRFMT
                     HGGKFMILHSEHKIKRHIKVIGTKSPYNSDWEYWTTRLGKMPGIKPKLAKLMKMQQGR
                     CGKCKLFLNSDNVIEIHHNDGNHGNNKYENLVLLHGHCHDIIHSKGMYDKHQISRGAV
                     "
     gene            complement(2084..2218)
                     /locus_tag="PLS16_10955"
     CDS             complement(2084..2218)
                     /locus_tag="PLS16_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61646.1"
                     /translation="MRNRVDKYALPKGKGIIEIRMVESDLMFLNILGGKQAPNLNMKL
                     "
BASE COUNT          670 a          501 c          385 g          941 t
ORIGIN      
        1 atgcaaatgc agcaagtaaa gaagcagtaa aagggtcagt tttaggaaaa aacagctcat
       61 taaggagaac agccatatga acaaataaca taagttatct aagtagagat agtcacccat
      121 ctctactcga cttcaaaacc gtacgtgaca gtttcccgtc atacggctcc tcgactgatt
      181 tggtgtttgt catacatacc tttgctatgt ataatatcgt ggcaatgtcc atgtaataac
      241 actaaattct catatttatt gttaccatga tttccatcgt tatgatgaat ttctataaca
      301 ttatcagaat taaggaataa tttacatttt ccacatctac cttgttgcat tttcattaat
      361 ttcgctagct ttggttttat tcctggcatt tttccaagac gtgttgtcca atattcccaa
      421 tcgctattat atggtgattt agttcctatg actttgatgt gacgtttaat tttatgttcc
      481 gaatgaagga tcataaactt gccaccatgt gtcataaatc tccatttata gttgccatgt
      541 tgtctaaaat atttgaattg tacaaaagcc ctagacttat caggatgtct ccttattgac
      601 cattgccaca atttcttatg aagcttgtga tccatcttcc taaagatact acttgatacc
      661 cctggtgtgt agtaattact ccatcctttt attattggat tgagtttttc tattactttt
      721 tcctgaggca ttgctatcat attccttaag atttttttga tagttaacaa atgctctttt
      781 tgtccttgtt tacttggtct agtataagac ttatagcctc ttcttttatt tttatctgga
      841 aattgcctta ttgtgtatcc taaaaagtca aatcctggtt tttgaccatt atcgctattt
      901 aaactatgca caatatttgt cttcgattct tttagtttta gcccgatgtt tgctaaccat
      961 ttttctatgg ttgcttttgc cttcataata atttccttat tttcgtgaat aacaacaaaa
     1021 tcatccgcat aacgaattat gctaattgct gtttgagcct tgatgttact acttcttcca
     1081 gtcttctttt taaaatcttc aagaagatct ttagctaaag aattttttgt atctttttct
     1141 aacccgtgaa gtgcaacatt tgcaagtaga ggagatatta ctccgccttg cggtgttcca
     1201 ttcttggtag tataaaatac ttctccatca attactccgg cttttaacca ccctttaata
     1261 attcgtcttt tcatcggggt agtggcaagc ttatttagca atacttggtg atttatattg
     1321 tcaaaacacc cagatatgtc tgcatctagt acataacctg tttttttcgt aattgcatta
     1381 tgtatggcga caattgcatc gtgcccagac ctgccaggtc tgaaaccata agagttaggc
     1441 tcaaattttg cctcccactc aggttccagt gccatcttta aaagtgcttg ttttgctcta
     1501 tcttctatcg taggtatacc aagaggacgt ttttcattag tcccactttt cgcaatccag
     1561 attcttctta atggtttgct ccttcccttt aaattaattg ataaagctaa gttcatcctt
     1621 tctctttggg taagagctat ttttccatct atcccagctg tcttgcgtcc tctgttatct
     1681 tggcttacct tgcgagtagc gagtattctt gcacttgttg acgctaacat caatctttgt
     1741 aacttatgca caagtttgat attatcttgt tgcatggctc gataaattcg tttttgtagc
     1801 ttaaatgttt taatttctag ctttcgccaa ggaatctcat tccattcata cctaatcata
     1861 atgattggtt cataacctat taactactac taacaacttt accatccaaa ccatcgtgtc
     1921 tccgtctgct tatcctaggt attaccctag gcatttgctt ctgagacaat ccctctaaca
     1981 tagagcttgc ggttggcacc ttcttcaata tgcttacatt atcaaaaata ggaaaatgta
     2041 ttgagcataa tgaagagcaa tatgttagtt accctgttct atattataat ttcatgttga
     2101 ggttaggtgc ttgctttcct ccgaggatat ttaagaacat caaatcagac tcgaccattc
     2161 tgatttctat tatcccttta ccttttggta atgcgtactt atcaactcta tttcgcattt
     2221 cgaatatgac gaaggttcta atacaaattt ctctcgtcac catactcaac tttgttcgag
     2281 aggattccca atagagtttt gagttactcc cttttatgcc atgcttgcac aattatattt
     2341 gccagttata atcgtgtggc atatactttt atcccgatgt ctgggaagat ggaatttaac
     2401 catcaatata atatagcttg tctttatctt tcttttaatc tgataaagaa gtttcaggtc
     2461 acacggtcaa aatactcgag gaatgtacct atagata
//
LOCUS       DAOQCW010000169         2493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_26648_length_2493_cov_5.439295, whole genome shotgun sequence.
ACCESSION   DAOQCW010000169 DAOQCW010000000
VERSION     DAOQCW010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26648_length_2493_cov_5.439295"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PLS16_10960"
     CDS             complement(<1..399)
                     /locus_tag="PLS16_10960"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNJ61647.1"
                     /translation="MNNLKIFGAELIDDKCIEQIKNCISDDDLAVLTADAHYGYGHPI
                     GGAVAYKDKVSLSGVGFDIACGNKAVRTDIKAEDINVPNIMDEIFRRISFGVGRYNNE
                     PVQHEVFDKIANAEFIPQRKLLQLAKDQLGT"
     gene            complement(419..494)
                     /locus_tag="PLS16_10965"
     tRNA            complement(419..494)
                     /locus_tag="PLS16_10965"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(456..458),aa:Pro,seq:tgg)
     gene            complement(711..1037)
                     /locus_tag="PLS16_10970"
     CDS             complement(711..1037)
                     /locus_tag="PLS16_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61648.1"
                     /translation="MKRQVKKVNTKSSEDIIEEEPKECFCGECICGESICGESLLRCE
                     SNQILPPPVSYEESRYGLFLTKKEWKKYIKYNGTIKFIPTHIGTRVLCKNDKNKWIDI
                     TDYKSW"
     gene            complement(1042..1353)
                     /locus_tag="PLS16_10975"
     CDS             complement(1042..1353)
                     /locus_tag="PLS16_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61649.1"
                     /translation="MNKQEQIRIFEENVAKMRDILLSKGDDYANEDRLSNFKLVGQIC
                     GLTPQQVCLVFMATKVVRLGNLVASGAGPKNESIYDSVLDLGNYNMLFDMINTEDFIK
                     V"
     gene            complement(1477..1704)
                     /locus_tag="PLS16_10980"
     CDS             complement(1477..1704)
                     /locus_tag="PLS16_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61650.1"
                     /translation="MEIIKKFTAVQISTKTINNVVMAELEYGRISGPYYDVTEPETEF
                     DTEQEAIEWAYKEEKYADWLILTKVSFKSEY"
     gene            complement(1796..>2493)
                     /locus_tag="PLS16_10985"
     CDS             complement(1796..>2493)
                     /locus_tag="PLS16_10985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNJ61651.1"
                     /translation="EFEEIAKELYRVTKQGGVVVWVVGDSTIKGSETGTSFKQALSFM
                     QCGFNLHDTMIYQKSGAGAVGSNKCYIQNFEYMFVFSKGKIKTTNLIEDRKNKKVCTT
                     IANVSRDKNNADNERKYRTITTKEYGKRFNIWQIGQRNNQDEFHLLHPAPFPQQLAND
                     HIISWSNEGDVVYDPFMGSGTTAKMAMLNNRNYIGSEISKEYCEIAEQRLKMCGGLFS
                     NSFQTELSNEAGT"
BASE COUNT          712 a          525 c          350 g          906 t
ORIGIN      
        1 agtgccaagc tgatcttttg caagctgcaa gagttttctt tgtggaataa attcagcatt
       61 agcaatttta tcaaacactt catgttgaac aggttcatta ttgtaccttc caacaccaaa
      121 agatattcgc ctgaatatct catccattat attaggaaca ttgatatctt ctgcctttat
      181 atcggttctt acagctttat tgccacaagc tatatcaaag ccaacaccag acaaagaaac
      241 tttatctttg taagccacag caccaccaat aggatgccca tacccataat gggcatcagc
      301 agttaataca gctaagtcat cgtcagatat gcagtttttt atctgctcta tgcatttatc
      361 gtcaattagt tcagcgccaa atatttttaa attgttcatt ttattatctt aaaaattgtc
      421 gggaaggcag gattcgaacc tgcgaactct tgaatccaaa tcaagcgagg acgacctgac
      481 tcctccactt cccgatatgt gtctttccac atcgtcatta tgtactggtg tccagttatt
      541 cccacctcgg ggacactcca cgagactcat catgagtttc tggcatttca gccgttatca
      601 accgttttta aaataggtgg ccagatgtta gcagggtaca taaatcacta acccacgcat
      661 ctttaatatc tttgtttaat gtaaacacct ttattataaa atagttgcat ttaccaagat
      721 ttataatcag ttatatcaat ccacttattt ttgtcatttt tacacaagac tcttgttcct
      781 atatgcgttg gaataaattt tatagtaccg ttatacttaa tgtatttctt ccattctttt
      841 ttggttaaga ataagccata tcggctttct tcataagaaa caggaggtgg aagtatctgg
      901 ttagattcac atctcaaaag gctctctcca caaatgctct ctccacaaat gcattctcca
      961 caaaagcact cttttggctc ttcttctatt atatcctcag aacttttggt atttactttt
     1021 tttacttgtc ttttcatagt tttatacttt aataaaatcc tctgtattta tcatatcaaa
     1081 aagcatatta tagttgccta aatccagcac actgtcatat atagactcat tctttgggcc
     1141 tgctcctgac gctactaagt ttcccaatcg aacaaccttt gttgccatga atactaagca
     1201 tacctgttgg ggtgtgagtc cacagatttg accaaccaat ttgaagttag ataatctatc
     1261 ttcattggca tagtcatcac ccttggacag aagaatatct ctcatcttgg ctacattctc
     1321 ttcaaaaatc ctaatttgtt cttgtttgtt catatacttg tatatttttc tcttaacata
     1381 tttagtctat tgaaaaaagg aagggattct tcatccacta cttcaaacgc tatttctgta
     1441 cgcatatagc aaaatgccaa attccgttat gcagggctaa tactcgcttt taaaagaaac
     1501 tttagtcaat attaaccaat cagcatattt ctcttctttg taagcccatt caatagcttc
     1561 ttgttctgtg tcaaactctg tttctggctc tgtaacatcg taatatggtc cagaaattct
     1621 accatattca agttctgcca ttactacatt atttattgtt ttagtactta tttgtactgc
     1681 tgtaaatttt ttaataattt ccataataaa tttagttttt aaaaacccgt ccaacacata
     1741 acagagtata agcggacatt aaaacgtcgc ttatacgggt gttataagca agctgctacg
     1801 ttcctgcttc gtttgacaat tccgtttgaa aagaatttga aaaaagccca ccgcacattt
     1861 taagacgttg ttcagcaatt tcacaatatt cttttgatat ttcactccct atgtaatttc
     1921 ggttattcag catcgccatt ttagcagtag ttccgctacc cataaaaggg tcataaacaa
     1981 catcaccttc attactccaa ctaattatgt ggtcgtttgc aagttgttgt ggaaatggag
     2041 caggatgcaa taaatgaaat tcatcttggt tattcctttg tcctatttgc caaatgttaa
     2101 atctttttcc atattctttt gttgttatcg tcctgtattt tctttcattg tcagcattat
     2161 ttttatccct gcttacattc gcaatagtgg tacaaacttt tttgtttttt ctatcttcta
     2221 taaggtttgt cgttttaatt tttcctttac taaaaacaaa catatactca aagttttgta
     2281 tataacattt attgcttcca actgcacctg ctccactttt ttgataaatc attgtgtcgt
     2341 gtaggttaaa tccgcattgc ataaaggaca aggcttgttt aaaacttgtt ccagtttcgc
     2401 taccttttat cgtactatca ccaaccaccc aaaccacaac accaccttgt tttgttaccc
     2461 tataaagttc tttggcaatt tcttcaaatt caa
//
LOCUS       DAOQCW010000170         2333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_30145_length_2333_cov_5.366550, whole genome shotgun sequence.
ACCESSION   DAOQCW010000170 DAOQCW010000000
VERSION     DAOQCW010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30145_length_2333_cov_5.366550"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            244..342
                     /gene="ffs"
                     /locus_tag="PLS16_10990"
     ncRNA           244..342
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLS16_10990"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            371..700
                     /gene="yajC"
                     /locus_tag="PLS16_10995"
     CDS             371..700
                     /gene="yajC"
                     /locus_tag="PLS16_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNJ61652.1"
                     /translation="MFQLFILQQQAAPGNSTFTLIMFGLMFVVFYFFLIRPQSKKAKE
                     QEKYIADLKVGDKIVTIAGIHGKIVAVNEGDNTFSIQVDSTTKMKIERSSISLEMSKK
                     VQDSPAS"
     gene            706..1299
                     /gene="coaE"
                     /locus_tag="PLS16_11000"
     CDS             706..1299
                     /gene="coaE"
                     /locus_tag="PLS16_11000"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNJ61653.1"
                     /translation="MLKIGVTGGIGSGKTTVCLLFQQLFNIPVYYADVRAKHIINENQ
                     TIKDQIIALFGEDAYLNNEYNRTFIASKVFNNNTLLQQLNAIVHPAVFKDAQHFFNKH
                     QSENYVLYEAAILFESGGYKTMDKNILVIAPTELRIERTMNRDGITSDKVKQRIKNQK
                     PDEEKIKLSNFVIVNDGEKDLKSQVQEIHRLILESIK"
     gene            1334..2266
                     /gene="trxB"
                     /locus_tag="PLS16_11005"
     CDS             1334..2266
                     /gene="trxB"
                     /locus_tag="PLS16_11005"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNJ61654.1"
                     /translation="MAEEIIDVLIIGAGPAGYTAAIYAARANLKPVLYTGIEPGGQLM
                     TTTDVENYPGYPDAVTGPKMMSDFQSQAERMGTDIRFGTVSKVDFSSKIHTVWIDDEK
                     ELKAKTVIIATGAKAKWLGLESETRLNGNGVSACAVCDGFFYKGKEVAIVGAGDTACE
                     EALYLSKLCPTVHMIVRKDEMRASKVMQDRVINTQNIKIYWNTETLEVLGGQKVEAIR
                     VKNNQTKEEQEIKLEGFFVAIGHEPNSKVFAPFLDVDDVGYIITEAKSSKTKVAGVFA
                     AGDVQDRIYRQAVTSAGSGCMAALDAERYLAEHF"
BASE COUNT          865 a          296 c          442 g          730 t
ORIGIN      
        1 gtatcaaaat tcatgctaaa aaagatatgc gtataatcat ttgttgaaaa ataactcaaa
       61 tgtaatgttt tagtatatat cttataatct tattgtagct gtaagttaag cctactcgct
      121 attaacatca attaacaact ttttattgtt tgtatatatg gaagttttat ttagtgagtt
      181 tgatgtaatt ttaaattatt gtttattgat acttaattag taaaaaaatg tatctttgca
      241 ctcgggcaag tctcatacga ccagctcctt acaaatcctc cagggtcgga aggcagcaaa
      301 ggtagtcggt tgtagcggtg cgatatgagt aacttgcctt tcttttttat attgaaaatc
      361 taattatatc atgttccaat tatttatatt acagcaacaa gcagctccag gaaactcaac
      421 atttacatta attatgtttg gattgatgtt tgttgttttt tatttctttt taattcggcc
      481 tcaatctaag aaggcaaagg agcaagaaaa atatattgct gacttaaaag taggtgataa
      541 gattgtaaca attgctggta ttcatggaaa aattgttgcc gtaaatgaag gagataatac
      601 attttctatt caagtggata gtacaacaaa aatgaaaata gaacgtagtt ccatttcttt
      661 agaaatgagt aaaaaagttc aagacagtcc tgcatcctaa ttgctatgct aaaaattggt
      721 gtaacaggtg gcataggtag tggaaaaact acagtttgtt tgttatttca gcaacttttt
      781 aatattccag tttattatgc cgatgttcgt gcaaaacata tcattaatga aaatcaaacg
      841 ataaaagacc aaattattgc attatttggc gaagatgctt atctaaacaa tgaatataat
      901 agaacgttta ttgcttcaaa ggtttttaac aataatacac ttttacaaca attgaatgca
      961 attgtacatc ctgctgtgtt taaagatgca caacattttt ttaataaaca tcaatcagaa
     1021 aattatgttt tatatgaagc tgctatttta tttgaaagtg gaggctacaa aacaatggat
     1081 aaaaatattt tagttattgc acccacggaa ttgaggattg aaaggacaat gaatagagat
     1141 ggaataacat cagataaagt taagcaaaga attaagaacc aaaaaccaga tgaagaaaaa
     1201 ataaaactta gtaactttgt gattgtaaat gatggagaaa aagacttgaa atcacaagtt
     1261 caagaaatac atcgtttaat tttagaaagt ataaaataaa gatagaattt ataacagata
     1321 aaaaatacaa aaaatggcag aggaaattat tgacgtatta attattggag caggaccagc
     1381 aggttatacc gccgcaattt atgcggcaag agctaattta aaaccagtac tttatacagg
     1441 aattgagcca ggaggacaat taatgacaac gacagatgta gaaaattatc caggatatcc
     1501 agatgctgta actggaccca aaatgatgtc tgattttcaa agtcaagctg aaagaatggg
     1561 tacggatatt cgttttggaa cagtaagtaa agtagatttt tctagtaaaa ttcatacagt
     1621 ttggattgat gatgaaaaag aattaaaagc aaaaacagta attattgcaa caggtgcaaa
     1681 agcaaaatgg ttgggattag aatctgaaac gagattgaat ggaaatggag tttctgcttg
     1741 tgctgtttgt gatggttttt tctataaagg taaagaggtt gctattgttg gagctggaga
     1801 caccgcatgt gaagaagctt tgtatctttc aaaactttgt ccaacagtac acatgattgt
     1861 ccgaaaagat gaaatgcgtg catctaaggt tatgcaagac agggtaataa atacgcaaaa
     1921 tattaaaatt tattggaata cggaaacttt agaagtattg ggcggacaaa aggtagaagc
     1981 tattagagta aaaaataatc aaacaaaaga agagcaagaa ataaagttag aaggattttt
     2041 tgtagctata ggtcatgagc caaattctaa agtatttgca ccatttttag atgttgatga
     2101 tgtgggttat attattacag aagcgaaatc ttcaaaaact aaagttgcag gtgtttttgc
     2161 cgcgggtgat gttcaagata gaatttatag acaagctgta acatcggcag gcagtggctg
     2221 tatggctgcc ttagatgcag aacgatattt agcagaacac ttttgataaa tgattaaaat
     2281 catatttcta tttcaattta gttggatgtt tctaaagctg accgctaaca aaa
//
LOCUS       DAOQCW010000171         2244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_32424_length_2244_cov_6.394244, whole genome shotgun sequence.
ACCESSION   DAOQCW010000171 DAOQCW010000000
VERSION     DAOQCW010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32424_length_2244_cov_6.394244"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PLS16_11010"
     CDS             <1..542
                     /locus_tag="PLS16_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61655.1"
                     /translation="STYSRTQALPFEIYLLGTKQKPNPNLFNRKDVVLATLLEGDFTS
                     LFLNQYTSDLQSLLDKQGIEFRGKSYKNKMIVIADGDVIANDLDSKGSVIPLGYDRYG
                     QQQYANKDFILNCVEYLVDDNNLIEARNREVKMRLLDKAQLTEKKELWQFLNFAIPVF
                     FISIFGYIYFKQRQRKYAK"
     gene            574..1527
                     /gene="rsgA"
                     /locus_tag="PLS16_11015"
     CDS             574..1527
                     /gene="rsgA"
                     /locus_tag="PLS16_11015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNJ61656.1"
                     /translation="MELKTQQKGKVTQTTGSWYAVLLENGNTVQCRLRGKLKLQDLDT
                     TNPVAVGDFVLLENTLEDGEFVITDILERQNYIIRQSPRKEHQRHIIASNIDQFFIVA
                     TISQPRTSLGFVDRCIAAAECYHIPVHIIINKKDLLRKKDIEKLKEWQQIYHSIAYPI
                     HVISANDKADIEMLKNILKDKTTLISGHSGVGKSTILNAVNGDLRLKTGKISSKWEKG
                     MHTTTFATLYSIFENTFVIDTPGIKEFSILHVEPEEVSGYFVDIKEHAKSCKFNNCMH
                     INEPDCAVLSALDDGRLAPTRYENYLNIVENIKEINYWERK"
     gene            1673..>2244
                     /locus_tag="PLS16_11020"
     CDS             1673..>2244
                     /locus_tag="PLS16_11020"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNJ61657.1"
                     /translation="MLQATNLSIIFGSRILFDNVTFTIQPRDRIGLVGRNGVGKSTLL
                     KVINGEISPQEGGISKPNGYTIGFLKQEIRGQHEPTVWLEAQTAFKEINALQEQLDKI
                     QHELETRTDYDTDDYMNLSQSFCDLNDRLVHLDIDDKDKKTELVLLGLGYKREDFEKA
                     PSTFSGGWAMRLELAKLLLQNPDLLMLDEPT"
BASE COUNT          833 a          308 c          390 g          713 t
ORIGIN      
        1 cttctactta ttccagaaca caagcgctgc cttttgaaat ttatctttta ggaacaaaac
       61 aaaaacccaa tcctaattta ttcaatagaa aagatgtggt gttggcaaca cttttagaag
      121 gcgattttac ttctcttttc cttaatcaat atacttctga tttacaaagt ttgttggaca
      181 agcaaggcat tgaatttaga ggaaagagtt acaaaaacaa aatgattgtg attgcagatg
      241 gcgatgtaat agccaatgat ttagacagca aaggttccgt aatcccactt ggttatgaca
      301 gatatggaca acaacaatat gcgaataaag attttatttt aaactgtgta gaatatttgg
      361 tcgatgataa taatttaata gaagccagaa atagagaagt aaaaatgcga ctattggaca
      421 aagcccaatt aacagagaaa aaagaacttt ggcagttctt aaattttgca attccagtat
      481 tttttatttc tatctttgga tatatctatt ttaaacaaag acaaagaaag tacgcaaagt
      541 aaaaaaggag aatatatttt ccaatccgat ttcatggaat taaaaacaca acaaaaaggc
      601 aaagtaactc aaaccacagg aagttggtat gctgtattgt tggaaaatgg gaatacagtc
      661 caatgtcgtt tgagaggaaa actaaaatta caagatttag ataccacaaa tccagttgca
      721 gttggagatt ttgtcctatt agaaaacaca ctagaagatg gagaatttgt aattactgat
      781 attcttgaaa ggcaaaatta tattatacgt caatcaccga ggaaagaaca ccaaagacat
      841 atcatagcat caaatatcga ccagtttttt attgtagcaa ctatctcgca acctcgaact
      901 tcattaggtt ttgtggacag atgtattgcc gcagctgaat gttatcatat acctgtgcat
      961 attattatta ataaaaaaga tttactgcga aaaaaagata tagagaaatt aaaagaatgg
     1021 caacaaattt atcattctat tgcttatcct attcatgtaa taagtgcaaa tgataaggct
     1081 gatattgaaa tgctaaaaaa tattttaaaa gataaaacta ctttaatttc agggcattct
     1141 ggcgtaggaa aatctacaat attgaatgct gtgaatggag atttacgact aaagacaggc
     1201 aaaatttcaa gtaagtggga aaaaggaatg cataccacca cttttgctac actttattca
     1261 atttttgaaa atacttttgt gatagataca ccgggcatca aagaattttc tattctacat
     1321 gtagagccag aagaagtatc aggttatttt gtagatatta aagaacatgc aaaatcttgt
     1381 aaatttaata attgtatgca cattaatgaa cctgattgtg cagttttatc ggcattagac
     1441 gatggtagat tagcaccaac tagatatgaa aattatctaa atattgtcga aaatattaaa
     1501 gaaattaatt attgggaaag gaaatgagtt atttcaattc tttacacaac ttcaatttat
     1561 atttgttttt ttaaaatttt acttcgagta gcatatatta aaaaacaaac aaattcaact
     1621 aattcaggcg tttagttttt atttggtgct aggaatatat tatttttgcg gcatgttaca
     1681 agcaacaaat ttgtctatta tttttggaag tcgtatttta tttgataatg ttacttttac
     1741 tattcaacca cgcgatagaa ttggtttagt tggtcgaaat ggtgttggaa aatctacttt
     1801 attaaaagtt ataaatggtg agatttctcc tcaagaaggt ggcatttcca aaccaaatgg
     1861 ttatacaatt gggttcttga aacaagaaat tcgtggacaa cacgagccaa ctgtttggtt
     1921 ggaagctcag actgcattta aagaaattaa tgcattacaa gaacaattgg ataaaattca
     1981 acatgaatta gaaacaagaa cggattacga tacagatgat tatatgaatc tttctcagtc
     2041 tttctgtgat ttgaacgata gattggttca tttagatatt gacgataaag acaaaaaaac
     2101 ggaactcgta ttattaggat taggttataa aagagaagat tttgaaaaag ctccaagtac
     2161 tttttctgga ggttgggcaa tgcgtttgga attagcaaag ttattgttac aaaatcctga
     2221 tttactaatg ctcgatgagc caac
//
LOCUS       DAOQCW010000172         2229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_32825_length_2229_cov_5.181693, whole genome shotgun sequence.
ACCESSION   DAOQCW010000172 DAOQCW010000000
VERSION     DAOQCW010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32825_length_2229_cov_5.181693"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..1518)
                     /locus_tag="PLS16_11025"
     CDS             complement(148..1518)
                     /locus_tag="PLS16_11025"
                     /inference="COORDINATES: protein motif:HMM:NF018269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HNJ61658.1"
                     /translation="MKRLTPIVLYIILVMTSYSCKKDTNITNVFSEEFPGGETTYPNI
                     FSGVFEQPSPNLNATELAQHGKGDVNFSAIFVTSPAIKNYGLGPIFNHNSCVSCHVKD
                     GRTPQPFSGDDLKGLLLRLSIPGTDPHGGPLDAPGFGGQLQNKGIVGTQAEAKVQLSF
                     VETSYTFDDGTPYTLRKPIYTLVNSYLPLPGSLMVSPRIGPQIYGLGLLEAIKENDIL
                     SKEDVSDADGDGISGKANRVWNPVTQKVELGRFGWKANAPNLLVQSAGALQQDMGITS
                     FVFPQEASYNQSQYDGLLDDPEIDSQFLFDVTFYTQTLAVPIRRDFNHPSVLNGKQLF
                     MKIGCNKCHSPGYITGVGATQALNQQVIYPYTDLLLHDMGADLADNRPDFLADGNEWR
                     TPPLWGIGLFPMVNGHSNLLHDGRARNVLEAIMWHGGEAEQQKQKVKKLSAKEREDLV
                     KFVNSL"
     gene            complement(1532..>2229)
                     /locus_tag="PLS16_11030"
     CDS             complement(1532..>2229)
                     /locus_tag="PLS16_11030"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HNJ61659.1"
                     /translation="YIDQLTGDAGTGLKGFHAIEYILWGSDGNRAAGSFSARELEYLV
                     ACAESLKGQAETLLNAWTSGGYANELKSQNGVYSSEKAVLIELAEGIRGIAGEVWNTK
                     IQNTLDGYHADGVEGGIREEESRFSNNTKLDFTNNIISIKNVYSGMYGTKGNGNGLNK
                     VVKQLDQNLANKIATQINEAIAAIQNIGGNTSTTYSQALASDMASIETAQEKVEALET
                     TIQNELLPLINKL"
BASE COUNT          683 a          417 c          334 g          795 t
ORIGIN      
        1 tctgcttctg gcatgtctct ctttgttgag actaaatatt tcaacctata attattaaat
       61 taaagtaatg cattagcatc aatttctaac gaattggaac tgtgtcgtat aatttcttaa
      121 agttgaggta atgtactgat atatcgttta taaagaattt acaaatttta ctaagtcctc
      181 tctttctttt gcagataatt ttttgacttt ttgtttttgt tgttcagctt ctccgccatg
      241 ccacataatt gcttctaata catttcttgc tcgtccatca tgtaataaat ttgaatgacc
      301 atttaccatt ggaaatagtc caattcccca aagtggtggc gttctccatt cattaccatc
      361 agctaaaaaa tcaggtctgt tgtctgctaa atcagcaccc atatcgtgta ataacaaatc
      421 tgtataagga taaataactt gttgatttaa tgcttgtgtt gctccaactc cagtaatata
      481 tccagggcta tgacatttat tgcagccaat tttcataaac aattgcttgc cgttcaaaac
      541 ggaaggatga ttaaaatctc gtctaatggg aacagctaaa gtttgcgtat aaaaagtaac
      601 atcaaataga aattggctat caatttcagg atcatctaat agtccatcat attgtgattg
      661 attataacta gcttcttgtg gaaaaacaaa agaggtaatg cccatatctt gttgtaatgc
      721 accagcagat tgtactaata aattcggagc attggctttc caaccaaatc ttccaagttc
      781 aactttttga gttacgggat tccaaactct attagctttt ccagaaatgc catctccgtc
      841 tgcatctgaa acatcttcct tagataaaat atcattttcc ttaatggctt ctagtaaacc
      901 taaaccataa atttgaggtc caattcttgg agataccatt aatgaacctg gtaaaggtag
      961 ataagaattt acaagtgtat aaatcggttt tcttagtgta tatggcgttc catcgtcaaa
     1021 cgtataactt gtttcaacaa atgacaattg aacttttgct tcggcttgtg tgccaacaat
     1081 acctttgttt tgtaattgac caccaaagcc cggtgcatca agtggcccac cgtgaggatc
     1141 tgtacctgga atacttaatc ttaataataa tccttttaaa tcatcgccac taaaaggttg
     1201 tggagtcctg ccatctttga catggcagga aacacatgaa ttatgattga aaattggtcc
     1261 taaaccatag ttttttatag ctggacttgt tacaaatatt gcactaaaat tgacatctcc
     1321 tttgccatgt tgagctaact ctgttgcatt tagattcgga gaaggttgtt caaaaactcc
     1381 actaaaaata ttagggtagg ttgtttcgcc tccaggaaat tcctccgaaa atacattagt
     1441 aatatttgta tcttttttac aagaataaga ggtcattact aagatgatat ataatacaat
     1501 tggagttagc cttttcatag aaaagatttt tttataattt attaattaat ggcaaaagct
     1561 cattttgaat agtagtttct aatgcttcaa ctttctcttg agcagtttca atagaagcca
     1621 tatctgatgc taatgcttgg gaataagttg tacttgtgtt gccaccaata ttttgaatag
     1681 cagcaattgc ttcatttatt tgagtagcga ttttattggc taaattttgg tctaattgtt
     1741 ttacaacttt atttaatcca ttaccatttc ctttagttcc atacattcct gaatatacgt
     1801 ttttgatact gataatatta tttgtaaagt ctaattttgt attgttacta aatctacttt
     1861 cttcttcacg aataccacct tcaacgccat cagcatgata accatctaat gtattttgaa
     1921 tttttgtatt ccaaacttcg ccagcaatac cacgaatacc ttctgcaagt tcaattaata
     1981 cagctttttc tgaagaataa acaccatttt gggattttaa ttcattagca taaccaccac
     2041 tagtccatgc attcaataag gtttctgctt gtccttttaa actttcagca caagctacta
     2101 aatattctaa ctctcttgca gaaaaactac ctgcagctct atttccatca cttccccata
     2161 aaatatattc aatggcatgg aaacctttta atccagttcc tgcatcgcct gttaattggt
     2221 ctatatatg
//
LOCUS       DAOQCW010000173         2186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_34007_length_2186_cov_5.778508, whole genome shotgun sequence.
ACCESSION   DAOQCW010000173 DAOQCW010000000
VERSION     DAOQCW010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34007_length_2186_cov_5.778508"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1186)
                     /locus_tag="PLS16_11035"
     CDS             complement(<1..1186)
                     /locus_tag="PLS16_11035"
                     /inference="COORDINATES: protein motif:HMM:NF019735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61660.1"
                     /translation="MLLKNKYFISLVVVSVLTFISITCVLSKNNSNKANKTTSPLYQK
                     TLLKIVYNDTVDANRLQKFVSLFDTQTGIWTDIDYTDDARTNWDPIAHLARIRYLAID
                     YSKNQNKKYLEVINLGLEKWFQEDYTCPNWFVNDITAKNHLVDILVAVGTNITPSNYN
                     NILKKLDALYPEKRTYVNRVYLATIQLKYALYFNDTTLINNAKELINNEIYISNDVEG
                     LQADYTYRFHKARIQEYAYGKGFLESCVDVFELLNQTPYRFSDNKIAILRNFILESWQ
                     WLARGSYTVPSTMDRSSSRKGALKAPQIIETLERFKKIDTLYVLDYQKIIEQQNNIKN
                     FNLVGVKSFPYSDIAVAHSSNASFFIKVLSDRNLPTESINSENLLGKFLHFGNHFIIK
                     DAI"
     gene            complement(1170..>2186)
                     /locus_tag="PLS16_11040"
     CDS             complement(1170..>2186)
                     /locus_tag="PLS16_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HNJ61661.1"
                     /translation="KLLIDIKHPAQLNLFKNLAIDLKNENWDITICYLQRGKIPKIIE
                     KEFQGFHTIPVGSSKGTKWSIIWNGNIVRILTFLKLIFQNKYNICISASSVPLALACK
                     LTNTPILQFYDDPERKSLTKINAFLSTKIYFPFIVEQTDKIEVFNCLKEWSYLSPKRI
                     NPSTKVLQEYNLEKHNYVFVREVSNKSFNYYGQDDSVVLSFADKIDKTVKIVLSLEDK
                     SIKDKFPNDWILLEEPVSDIHALIYYSKLVISSGDSMAREGAMLGVPSIYCGFREMKA
                     NNVLIKKGILVHKTENEAIEYINQKINSNFEDSNQVAFRSQLAEEWDDMIDFMKQKIN
                     AFKK"
BASE COUNT          727 a          341 c          291 g          827 t
ORIGIN      
        1 caatggcatc tttgatgata aaatgattgc caaaatgtaa gaattttccg agtaagtttt
       61 cagaattgat actttcagta ggtaagtttc tatcggataa tacctttata aaaaaagagg
      121 cattggaaga atgagcgact gcaatatctg aataaggaaa actttttaca ccaactaaat
      181 taaagttttt aatattattt tgttgctcga ttattttttg ataatctaaa acatataaag
      241 tatctatctt tttaaatcgt tctaatgttt ctataatttg tggtgctttt aaggcacctt
      301 ttctagaact agatctatcc attgtacttg gtactgtata acttcctctt gctaaccatt
      361 gccaactttc taagataaaa tttcttagaa tggctatttt attatctgaa aaacgataag
      421 gcgtttggtt taacaattca aaaacatcca cacaactttc taaaaaccct ttgccataag
      481 catattcttg aatacgtgct ttatgaaatc gataggtata atctgcttgc aatccctcca
      541 catcgttgga aatataaatc tcattattga ttaattcttt agcattattg attaaagttg
      601 tgtcattaaa atacaaagca tattttaatt gaattgtagc taaataaact ctattaacat
      661 aagttctctt ttctggataa agggcatcta gttttttaag tatattatta tagtttgatg
      721 gtgttatatt tgtacctact gccaccaaaa tatccaccaa gtgatttttg gcagtaatat
      781 catttacaaa ccaatttgga caagtataat cttcttgaaa ccatttttct aatccaagat
      841 taataacctc taaatatttt ttattttgat ttttggagta atcaatagct aaatatcgaa
      901 ttctagcaag atgtgctatt ggatcccaat tggttcgagc atcatctgta taatctatat
      961 ccgtccagat tcctgtttga gtatcaaaaa gcgatacaaa tttttgtagt ctattagcgt
     1021 caacagtgtc attataaaca atctttaata atgttttttg gtatagagga gaagttgttt
     1081 tattggcttt gttagaattg ttttttgaca ggacacaagt aatagaaata aaagttaaaa
     1141 cacttactac aacaagactt ataaaatatt tattttttaa aagcattaat tttttgtttc
     1201 ataaaatcaa tcatatcatc ccattcctca gcgagttggc ttctaaatgc aacttgattt
     1261 gaatcttcaa aattagaatt tattttttga ttgatatact ctattgcttc attttctgtt
     1321 ttatgtacta atattccttt ttttattaac acattattgg ccttcatttc tctaaatcca
     1381 caataaattg aaggaacacc caacattgca ccttctcttg ccatactatc tccagatgaa
     1441 ataaccaatt tagaataata aattaaagca tgaatatcag aaaccggttc ttccaataat
     1501 atccaatcat taggaaattt atctttgatt gatttatctt ctaatgagag tacaattttt
     1561 acagtcttat ctattttatc agcaaaactt aaaactacac tatcatcttg tccataataa
     1621 ttgaaagatt tattgctaac ttcccgaaca aagacataat tatgcttctc taaattatat
     1681 tcttgtaaca cttttgtact aggattaatt ctctttggac tcaaataact ccattctttc
     1741 aggcaattaa agacttctat tttgtccgtc tgttccacta taaatgggaa atagatttta
     1801 gtagataaaa aagcatttat tttagttaaa ctttttcttt cagggtcgtc ataaaattgc
     1861 aaaattggtg tatttgttaa tttgcaagct aaagccaacg gaacgctgct tgctgaaatg
     1921 cagatattgt atttattctg aaagattaat tttaaaaaag taagaatacg aacaatattg
     1981 ccgttccaaa taatagacca tttagtacct ttgctgcttc cgacaggaat agtatgaaat
     2041 ccttgaaatt ctttttctat tatcttaggg attttccctc tttgcaaata acaaatagtg
     2101 atatcccagt tttcattttt taaatcaatg gctaagttct taaataaatt caactgagca
     2161 gggtgcttga tgtctattag gagttt
//
LOCUS       DAOQCW010000174         2113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_36292_length_2113_cov_5.974733, whole genome shotgun sequence.
ACCESSION   DAOQCW010000174 DAOQCW010000000
VERSION     DAOQCW010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36292_length_2113_cov_5.974733"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            280..729
                     /locus_tag="PLS16_11045"
     CDS             280..729
                     /locus_tag="PLS16_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61662.1"
                     /translation="MKKIKIFSLVVFGSMLFASCSSTSLNKINTSVYDTRQQKINKNS
                     IPYNPVVVDLVVDINKKVSGSNLRQVDRYTDYELQNAKEAALYNAMTNSGADVIIDPI
                     YKINISNNDGRDAKITIQTEVTGFYAKYTNAHKADANEIQNLKILNK"
     gene            732..1391
                     /locus_tag="PLS16_11050"
     CDS             732..1391
                     /locus_tag="PLS16_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61663.1"
                     /translation="MNRIHKLLAIIIVALVFPSCSQMAYNKINTSVYETRQQDIDKGA
                     LPFNPVIADLQVDLSKKISGTSIRQVSRFNDLELENTKEAALYNAVTNSGADVVVDPI
                     FKINISNNDGRDEKVTIQSEVTGFFGKYTSIHKATTAELENAVYDRSLLYANTPTATN
                     KNSSAVLPIEEDMALAAEKAAALKKKKRKAIGWAVGSTLILSLTLGLGVGLGMGNSYG
                     F"
     gene            complement(1470..>2113)
                     /locus_tag="PLS16_11055"
     CDS             complement(1470..>2113)
                     /locus_tag="PLS16_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HNJ61664.1"
                     /translation="QKVAAELKAQAELKLKQDAELAALKKQQLEQEQKKAEETALLAK
                     EMVAKEPTLENIDINSKTDNSIFPNPKITNLVYSKKDDNLFFTDISNGIFTGSTTKNL
                     TIDIAGEESKIQFVKGKIPKLLIKTENVKPTADYINLYTCDFKKGNRQITVRPAKNRV
                     FILPSEISSNVYELVLPENLGEGEYVFVLQNDVNKPITINSTSTKVFCFGVKY"
BASE COUNT          764 a          308 c          321 g          720 t
ORIGIN      
        1 tttttacaat tcacaaaaat gctttttgaa actgctgtct aacataatta tgatgcaaat
       61 aaattcaata ataggagcca agtaaacaaa tctataagaa gcaacttcaa tataaaagta
      121 gtaaaaatca cagaagaact gcgacagaaa tatccatatt ttatagaata ttgttgataa
      181 ggagagatga aattggaatt ataaaaatta ttgaaataaa aactatgttt aagtttattt
      241 tgtatattag taaatatttt aaacacttaa ataaaaatta tgaaaaaaat taaaatcttt
      301 agccttgtcg tatttggttc aatgctattt gcgtcgtgtt caagtacatc attaaacaaa
      361 atcaatacga gcgtgtacga tactcgtcaa caaaaaatca acaaaaattc aattccatac
      421 aatccagtag ttgtagattt agtggttgat atcaacaaaa aagtatctgg ttctaatctc
      481 cgtcaagttg accgatatac agactatgaa ctccaaaatg caaaagaagc agcattgtat
      541 aatgccatga ctaattcagg agctgatgtg attattgatc caatctataa aattaatatt
      601 tcaaataatg atggacgaga tgctaaaata accattcaaa cagaagttac tggcttctat
      661 gcaaaatata caaatgctca caaagcagat gctaatgaaa ttcaaaattt aaaaatacta
      721 aataagtaac catgaataga atacataaat tacttgcaat aattattgtt gcattagtct
      781 ttccttcctg ttctcaaatg gcatacaaca agattaatac atcggtgtat gaaacacgtc
      841 aacaggacat tgataaagga gctctacctt tcaatcctgt aattgcagat ttacaagttg
      901 acctatccaa aaaaatatct ggaacttcga ttcgacaagt tagtagattt aatgatttag
      961 aattagaaaa cacgaaagaa gcagcattat ataatgcggt aactaattct ggcgctgatg
     1021 ttgttgttga tccaatattt aaaattaaca taagcaataa tgatggcaga gatgaaaaag
     1081 taactattca atcagaagtt actggtttct ttggaaaata tacaagcatt cataaagcaa
     1141 caactgctga gttagaaaat gctgtatatg atagatcttt gttgtatgca aatacaccaa
     1201 cagcgacaaa taaaaattcg tcagcagttc ttccaataga agaagatatg gcattagcgg
     1261 cagaaaaggc agctgcatta aagaagaaaa aacgaaaagc aataggctgg gctgttggct
     1321 ctacattaat tttaagttta acacttggat taggtgttgg attaggaatg ggaaattctt
     1381 acggttttta attcctgcat ataatcattc taaaaataca aagaggctgt ttcactaatg
     1441 aaacagcctc tcttttattt caatttaagt taatatttta caccaaaaca aaatactttt
     1501 gtagaagttg agtttattgt tattggttta tttacatcat tttgtaatac aaaaacatat
     1561 tcaccttcac ctaaattttc tggtaatact aattcatata cattagaaga aatttcacta
     1621 ggtaaaataa acactctatt tttcgcaggt cggactgtaa tttgtctatt tcctttcttg
     1681 aaatcacaag tatatagatt gatatagtca gcagttggtt ttacattttc agttttaatt
     1741 aataatttag gaattttgcc tttcacaaat tgaatttttg attcttctcc agcaatatca
     1801 atagtaaggt ttttagttgt actgcctgtg aaaattccat ttgaaatatc tgtaaaaaat
     1861 aaattatcat ctttttttga gtaaactaaa ttggtgattt tgggatttgg aaatatagag
     1921 ttgtctgttt tactattgat atcaatattt tctaaagttg gttctttagc aaccatttcc
     1981 ttagctagca atgcagtttc ttcagccttc ttttgttctt gttctaactg ttgttttttt
     2041 aatgcagcca actctgcatc ttgtttaagt tttagttctg cttgtgcttt tagttctgcg
     2101 gccacctttt gtt
//
LOCUS       DAOQCW010000175         2089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_37111_length_2089_cov_5.521632, whole genome shotgun sequence.
ACCESSION   DAOQCW010000175 DAOQCW010000000
VERSION     DAOQCW010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37111_length_2089_cov_5.521632"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            305..1723
                     /locus_tag="PLS16_11060"
     CDS             305..1723
                     /locus_tag="PLS16_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61665.1"
                     /translation="MRKYFTLLLVVLYSNTFAQKIKVDAQMIQMGWTFVSPLILKKIN
                     DPLTKEIISKTVPKLINQDAKGAAYEVSDAIYRIKNVKVLNKEFLSQVEKSITDGVKA
                     INRKDYASAVNGLVKTVAFSEAYIKKGILDQPTPPTQVTNASINAVKTDIVTAKEINE
                     KLHLGKNNDYLFFIPTGNFTQKETTEKENLFAEDFNVNIDSEKKFNMGVGRQISSDLQ
                     NVSVERFQTDKNASDKLKETMADILSKSLGEGQIIKSEIATYPNFKSLKYTYLYTDKE
                     TSTSSMAYVLITFQDTSMFIIYFNTPANDFLETSKSFEDLMRTFFIIGIDEISNANSK
                     TYSKAVITKIAINKIPSIKSTGEPWDNPFGNNLPDIYYIITDKNGQILRSLNPMERKE
                     DVNPQNLPVYFSLTGKGFVLDDLKETIFIFLYDYDSVTDPDKVGSVGFKLSDYMFGKD
                     AYKEQITKANGNTEISISVKWE"
BASE COUNT          788 a          360 c          324 g          617 t
ORIGIN      
        1 agttgctgtt tctttcctta aatcaaatga aattaattta tatttgtaaa tgcttctgaa
       61 attatttttt tcacagactg atgttagcgg caatgttaac cactccgatt gaacgttact
      121 gctaagcgaa aatgacgagc tcagaaagtc accaaaaccg ttttggctat cgcttcttta
      181 aaataaaaag agaagcaacg cttgcacaca agcataccat tcttttactt cacattttac
      241 tttaccctgc tgcactaccg ttgtcacaag atatttacat ttgacttttt aaaaccaaaa
      301 taaaatgaga aaatacttta cactgctttt ggtagttctt tattctaaca cgtttgcaca
      361 aaagattaaa gtggatgcac aaatgataca aatgggttgg acatttgttt cgccactcat
      421 tttaaagaaa attaatgacc cactaactaa agaaattatt agtaaaacag tgccaaaatt
      481 aattaatcaa gatgcaaaag gagcggctta tgaagtctct gatgctattt acagaataaa
      541 aaatgtaaag gttttaaata aagaattctt atcgcaggtg gaaaaaagta ttacagacgg
      601 agttaaagca atcaaccgaa aagattatgc ctctgctgta aatggattag ttaaaacagt
      661 tgcattttcc gaagcataca taaaaaaagg aatactagac caacctactc caccaacaca
      721 ggttactaat gcttcaatca atgcagttaa aacagacatt gttacagcaa aagaaataaa
      781 tgaaaaatta catcttggga aaaacaacga ttatctgttt ttcattccaa ctggaaattt
      841 tactcaaaaa gaaactacag aaaaagaaaa tttatttgca gaggatttca atgtaaacat
      901 tgattcagaa aaaaaattca atatgggcgt tggaagacaa atatcatccg atttgcaaaa
      961 tgttagtgtt gagcgttttc aaactgataa aaacgcaagc gataaattaa aagaaactat
     1021 ggctgacatc ctttctaaaa gtttaggcga aggacaaatc ataaagtcag agatagcaac
     1081 ttatccaaat tttaaatcac ttaaatatac gtatctctat actgataagg agacttctac
     1141 ttcctcaatg gcatatgttc ttataacttt tcaagataca tccatgttta taatttattt
     1201 caacacacct gcaaacgatt ttctcgaaac ttcaaaatcc tttgaagatt taatgcggac
     1261 tttctttatt attggtattg atgaaatatc aaatgctaat tctaagacat attccaaagc
     1321 agtaatcaca aaaattgcaa ttaataaaat tccttcaata aaaagcacag gcgagccttg
     1381 ggacaatccc ttcggaaata atcttccaga tatttactac atcattacag ataaaaacgg
     1441 acaaatactt cgtagtttaa acccaatgga aagaaaagaa gatgttaacc cacaaaactt
     1501 gccggtatat ttttcgctca ctggaaaggg ttttgtatta gatgatttaa aagagacgat
     1561 ttttattttc ctatatgatt acgactcagt aactgaccca gataaagtcg gaagcgttgg
     1621 atttaaatta agtgattaca tgttcggaaa agatgcctac aaggaacaga ttactaaagc
     1681 gaatggaaat acagaaatct caattagtgt taaatgggaa tagtcttcaa gaaataccac
     1741 catgtttgct ttcacaatta aaagccttgc aacacgacac tggaaactca cctgtctaca
     1801 aaactcgcaa ctgcatagct tcgtctatta catttttcaa tcaaacagtt gtgttaaact
     1861 ctgacagtag attttcatgg aaacagtcgg gtgatacgta taaaatcaag tcaaccttgc
     1921 acaacccaat taaaagagta aaaagggata taaaacgatt ttattagctg cctttaaaca
     1981 aagcagctgt tttttattgt cggtttgtat tgagttccga acaaattgta ctgcaattgc
     2041 aacaaacaaa tattaaaaac aaagagccgc tgaaaaatac gcggctctt
//
LOCUS       DAOQCW010000176         1976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_41203_length_1976_cov_5.797501, whole genome shotgun sequence.
ACCESSION   DAOQCW010000176 DAOQCW010000000
VERSION     DAOQCW010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41203_length_1976_cov_5.797501"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="PLS16_11065"
     CDS             complement(<1..733)
                     /locus_tag="PLS16_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61666.1"
                     /translation="MTSFAQEVKLTWSPEIELKRKESISDIIHADKTGLMYTTVERKG
                     GMMGYYKPSTFKSVEKLSSNFSPVFSKEITAFDDDYAVQDFYYTKNHFLIFVNKHFKK
                     EDQTKYYVANMDMNGNINGNAKEYFTANKDDFSETASFSYYYSPDSSHLLAVAERSVS
                     FQGSGFGFKAIKAYKSNMKKEMKFEIIDIDNTGKKLWNKTIQIENKEDRSVVIEQKAI
                     NAKGDIFFLIKEYKSDKPKETIKDAN"
     gene            974..1969
                     /locus_tag="PLS16_11070"
     CDS             974..1969
                     /locus_tag="PLS16_11070"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HNJ61667.1"
                     /translation="MKHLTVKQRYQIEALYAQYPRVTQKQISKQLGITESQLSRELKR
                     NKMQNGKYKAENAQQYTDFRHHTKPKKKRLTPQLKLQIEQLLQQDLSPEQISCRLALE
                     GHQTVSHETIYLYIWQDKKQQGNLYQYLARKQRCRRKRGKKNDERGHIPNKTSIEQRP
                     QEANDRVRFGDFEQDTIIGANHKGAILTLVDRKTLFTKLVLLQGKNAEQLSVETIKTL
                     NHFKPYLHTITSDNGKEFSLHQHIAQQLEINYFFTHPYSSYECGTVENTNGLIRRYIP
                     KKSSFENLTQQTLDIIEHKLNNRPRKKLGFYTPNEILNAIFNNDKLILNKIAFIT"
BASE COUNT          690 a          391 c          290 g          605 t
ORIGIN      
        1 catttgcatc tttaatcgtt tctttaggtt tatcagattt atattctttt atcagaaaga
       61 atatatcacc tttagcattt atagcttttt gctcaataac tactgatcta tcttctttgt
      121 tttcaatttg aattgtttta ttccataatt tttttccggt attatcaata tcaataatct
      181 caaatttcat ttctttcttc atattacttt tgtatgcctt tattgcttta aatccgaatc
      241 cactaccttg aaatgaaaca ctacgttctg ctacggctaa taaatgagag ctatctggtg
      301 aataataata tgaaaagctg gctgtctcag agaaatcatc tttattcgct gtaaaatatt
      361 ctttagcatt cccattaata tttccattca tatccatatt tgccacataa tattttgtct
      421 ggtcttcttt tttaaagtgt ttgtttacaa aaatgaggaa gtgattttta gtataataaa
      481 aatcctgaac ggcataatca tcatcaaatg cagtaatttc tttgctgaaa acaggtgaaa
      541 aatttgaaga taacttctca accgacttga aagttgaggg cttataataa cccatcatac
      601 ctcctttacg ttcgactgtg gtgtacatta gtccggtctt atctgcgtga ataatatcag
      661 atatagactc ctttctttta agttcaattt caggactcca agtaagcttt acttcttgag
      721 caaaggatgt catacaaaag cactgaggat aacaaaaaat aatcttttca tattaagcgt
      781 gattgtttta tctgaacttg ttttatcttt gtacaaaatt atacatttat gcgagtaatc
      841 ctaacctaag ttcaagtgtt aaatgcaatt ttttgggggc ataaaataac attttttcaa
      901 aacagagagg aggttatcct ccctgctctg aatggatggt gttatattgt gccttcgtcc
      961 aaaaaattac aatatgaaac accttaccgt taaacaaaga taccaaatag aagctttata
     1021 tgcgcaatat cctcgtgtta cacaaaagca gatttcaaaa caactcggta tcaccgaatc
     1081 tcagttaagc agagagctga aacgcaataa gatgcaaaat ggcaaataca aagccgaaaa
     1141 tgcacaacaa tataccgact tcagacacca tactaaacct aagaaaaagc gacttactcc
     1201 tcagctaaaa ttacaaatag agcagcttct tcaacaagac ttatcacctg aacaaatcag
     1261 ttgtcgccta gctttagaag gtcatcaaac cgtaagccac gaaaccattt atctctatat
     1321 ttggcaagat aagaagcaac agggtaacct ataccaatac ttagcccgca agcaacgatg
     1381 ccgcaggaag cgaggtaaga aaaatgacga acgagggcat atccctaata aaacaagcat
     1441 agaacaacgt cctcaagaag caaacgacag ggtgcggttt ggcgattttg aacaagacac
     1501 gatcatcggt gctaaccaca aaggtgctat tcttaccctt gtagacagaa aaacgctgtt
     1561 caccaaatta gtcttattgc agggcaaaaa cgcagaacaa ctgtctgtag aaaccataaa
     1621 aaccctaaac cactttaaac catatttgca tacaattaca tcagataatg gaaaagaatt
     1681 ttcgctccat cagcacatag cccaacagct tgaaatcaat tatttcttca cacatcccta
     1741 ttccagttat gaatgtggca cggtagaaaa taccaatggg cttattcgta gatatatccc
     1801 caaaaaatca tcctttgaaa acctgacaca acaaactctt gacatcatcg aacataaact
     1861 caataacaga ccaagaaaaa aactcggatt ttatacccct aatgaaatac taaatgctat
     1921 ttttaacaac gataaactta ttcttaataa aattgcgttt attacttgaa ttcagc
//
LOCUS       DAOQCW010000177         1918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_43705_length_1918_cov_5.412238, whole genome shotgun sequence.
ACCESSION   DAOQCW010000177 DAOQCW010000000
VERSION     DAOQCW010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43705_length_1918_cov_5.412238"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="PLS16_11075"
     CDS             <1..796
                     /locus_tag="PLS16_11075"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HNJ61668.1"
                     /translation="IALERAGYEVIQYFASEIKPHAIRCTQSNYPNTIQLGSVLNVKG
                     SDLPKIDLLIGGSPCKGISRLNKNQEGLEHSESKLFWEYVRLLEEVKPKYFLLENTHG
                     NKEATEIITETLGVKPISINSKLVSAQNRPRYYWTNIPGVKQPADKGITTKDVFDYSG
                     ELADECRVKWLISESGKKSVGKGYTRVNPYPKSGCLTANGHKKWNENYLFKDGVYRYL
                     SQNELEKLQTLPIGYTSILNYDEAYDCIGDGWTVDIIAHILKYAVW"
     gene            964..1206
                     /locus_tag="PLS16_11080"
     CDS             964..1206
                     /locus_tag="PLS16_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61669.1"
                     /translation="MENHKGYELFNDASYYDMVCIRKSGSRDFNDTIHVSTMQEAKQT
                     VDEIIVLSDYMDWYTNKYGGVIMVSDYWIKEFVSGR"
     gene            1386..1670
                     /locus_tag="PLS16_11085"
     CDS             1386..1670
                     /locus_tag="PLS16_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61670.1"
                     /translation="MTKKEFVEAVASGLRKMNYTPDYLLIIADRFNDWEWDEEALCGI
                     KVIKSYISVNSGHSGHDYPVIPCFTDVSEKDIFMLVNYFQRGFEDSAGSF"
BASE COUNT          706 a          257 c          372 g          583 t
ORIGIN      
        1 aatagcgtta gaaagggcag gatatgaagt aatacaatac tttgcaagtg agataaaacc
       61 acacgcaata agatgtactc aaagcaatta tccaaacact attcaattag gtagtgtatt
      121 aaatgtgaaa ggtagcgatt taccaaaaat tgatttattg attggcggct caccttgtaa
      181 aggaatttca agattgaata aaaaccaaga aggacttgaa cactctgaaa gtaaattatt
      241 ttgggagtat gtaagattac ttgaagaagt aaagccaaag tattttttac ttgaaaacac
      301 acacggtaac aaagaagcta cagagataat cacagaaaca ttaggagtaa aaccgatttc
      361 aataaatagc aaattagttt ctgctcaaaa tagaccaaga tattactgga caaacatacc
      421 tggagttaaa caacctgcgg ataaaggaat tacaactaag gatgtatttg attattctgg
      481 tgaattagcc gatgaatgta gggtaaagtg gttaatttct gaaagtggta aaaaatcggt
      541 tggtaaagga tacacaaggg ttaatccata ccctaaaagt ggatgcttaa cagcaaatgg
      601 acataaaaaa tggaacgaaa attatttatt taaagacggt gtatatcgct atctttcaca
      661 gaatgaactc gaaaaattac aaacattgcc aataggttat acatccattc ttaattatga
      721 tgaagcgtat gattgtatag gtgatgggtg gacagttgat attattgcac acattctcaa
      781 atatgcagtg tggtagtatt gcctgtaacg ttgaagcatt ggcgaagaat gggaatatat
      841 ggcacttcgc ttaatgatag tacaagagtt aaattaataa acaaaggctc aataacattc
      901 gacagcccat tttttgccaa tgcaatgtta tgtgccgttt ttcttcacaa atcttaatta
      961 aaaatggaaa atcacaaagg ttatgaatta tttaatgatg catcatatta tgatatggtt
     1021 tgcattagaa agtcaggctc cagagatttt aatgacacaa ttcatgtctc aactatgcaa
     1081 gaagcaaaac aaacagttga tgaaattatt gtattgtcag attatatgga ttggtacaca
     1141 aataagtatg gtggtgttat tatggtttct gattattgga taaaagagtt tgtatctggt
     1201 cgttaaaatg gcacataacg attcggggct ttgcgtagta gcccttagta gaaacttaaa
     1261 attaaccacg acacttgata gggctattac gcaaaaccct tgttatgtgc cgtttttata
     1321 gaagattaga aagaagaccc acttccttta ggtgtgggag ttgtcaatca caaatcaaaa
     1381 ttaaaatgac aaaaaaagaa tttgttgaag ccgttgcttc tggtctaaga aaaatgaact
     1441 acacccccga ctatttacta attatagcag atagatttaa tgattgggaa tgggatgaag
     1501 aagccctgtg tggtattaag gtaataaaaa gttatatttc agtaaattct ggacatagtg
     1561 gacatgatta ccctgtaata ccttgtttta cggatgtatc agaaaaggat attttcatgc
     1621 ttgtcaacta ttttcagcga ggttttgaag atagtgcagg gtctttttaa aatggcacat
     1681 aacgtatata tgtaagaaac ttttataaac aaaacaaaat caatacttta aaaatgaatt
     1741 ttctaaaatg gatagataaa tattcgactg attataaact taaatagtat ggaattattt
     1801 gaaatatctg gtttttggaa cgatgttaaa actgaatttg aaggctacat tgtaagtcct
     1861 tctgacgaaa tagacgaagt aaatgatgac gatatatttt tttatggtct gtctgagg
//
LOCUS       DAOQCW010000178         1866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_46245_length_1866_cov_4.920486, whole genome shotgun sequence.
ACCESSION   DAOQCW010000178 DAOQCW010000000
VERSION     DAOQCW010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46245_length_1866_cov_4.920486"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..120
                     /locus_tag="PLS16_11090"
     CDS             13..120
                     /locus_tag="PLS16_11090"
                     /inference="COORDINATES: protein motif:HMM:NF017317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61671.1"
                     /translation="MQEGYLKRTPRGREATAKAYTHIGKVPPSQTGSLF"
     gene            complement(131..697)
                     /gene="ruvC"
                     /locus_tag="PLS16_11095"
     CDS             complement(131..697)
                     /gene="ruvC"
                     /locus_tag="PLS16_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNJ61672.1"
                     /translation="MAEKKQITILGIDPGSHTTGYGIINSDTRNAELILIGTIYTEKY
                     DDHYDRLCKIYDEVLEIINMYKPDVLAIEAPFFGKNVQSMLKLGRAQGMAIAAALSKK
                     IPIQEYSPKKVKQSITGNGNAAKEQVAAMLCQILKTNALPKQLDATDGLAVAMCHHFS
                     LLGLQNTTTKYSGWEAFAKANANKIIKK"
     gene            765..1727
                     /locus_tag="PLS16_11100"
     CDS             765..1727
                     /locus_tag="PLS16_11100"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HNJ61673.1"
                     /translation="MQNKYINFLIKLGVLIFLFVILYKQLFLNQSFKNAIQHFKISTD
                     GNVYLIYSVIILMIINWSVETIKWKFLIDKIHFISWLDALEGILFGITFSMFTPSRVG
                     EFGGRVFALNSDRIQAIVSTILGSATQLVANISLGVIGFLSYLLIFEKFEKYTLSIVF
                     FLALFLVLLLHICLFNLDIIATKFPKVKQLENIRKYIDIISLYSNIDLLKIELLSVVR
                     YIIYNVQFILLMYYFNIEISILYAVIISMTIFLFQTINPLNIALIDFGFRGNVALFFL
                     MPFTDNSLNILAATIFLWFVNLVIPAIFGGISALRFKFVSEEQQ"
BASE COUNT          592 a          254 c          285 g          735 t
ORIGIN      
        1 gaaccttttt taattcaaga aggttatctg aaaagaacgc ctagaggtcg agaggcaaca
       61 gcaaaagctt atacacatat tggtaaagtt ccaccgtccc aaactggtag tttgttttaa
      121 attctaagat ttattttttg ataattttat ttgcatttgc tttggcaaat gcttcccagc
      181 cagaatattt agttgtcgta ttttgcaaac ccaaaagaga aaaatgatga cacatagcga
      241 ctgccaaacc atcagtggca tctaattgtt tgggcaaggc attcgttttt aaaatctgac
      301 agagcattgc ggcaacttgc tcttttgcgg catttccatt tcctgtgata ctttgtttta
      361 ctttttttgg tgaatattct tgaatgggaa ttttttttga taaagcagca gcaattgcca
      421 tgccttgagc tcgacctaat tttaacatag attgtacatt ctttccaaaa aatggagctt
      481 caatcgctaa aacatcaggt ttatacatat tgatgatttc taaaacctca tcatagattt
      541 tacacaatct atcgtaatgg tcgtcatatt tttctgtata gatagttcca attaagatta
      601 attccgcatt tctagtatct gaatttataa taccgtaacc agttgtatga ctacctgggt
      661 caatacctaa aattgtaatt tgctttttct cagccacgct caaaattaat agttttagct
      721 tataaaataa aaaattactt atttttatcc aattgaaaaa agttttgcaa aataagtaca
      781 taaattttct gattaaatta ggtgttctaa tattcctttt tgtaatactt tataagcaac
      841 tattcctaaa tcaaagtttt aaaaatgcta tccaacactt taaaatttct actgatggaa
      901 atgtatattt aatatattca gtaattattt taatgattat aaattggagt gttgaaacca
      961 tcaaatggaa atttttgata gataaaatac attttatttc ttggttagat gctttagaag
     1021 gaatattatt tggaattacg ttctccatgt ttacaccaag tagagtagga gaatttggcg
     1081 gtcgagtttt tgcactcaat tcagatagaa ttcaagctat tgtttcaacc attttgggaa
     1141 gtgcaacaca attagtagct aatatatctt tgggtgttat tggttttcta tcttatcttt
     1201 taatctttga aaagtttgaa aaatatacat tgagtattgt tttcttttta gcattattcc
     1261 ttgttttatt attgcatatt tgtctattta atttggatat cattgctaca aaattcccga
     1321 aagtcaagca attagaaaat attagaaagt atattgatat tataagtctt tattctaata
     1381 ttgatttact aaaaatagaa ttgctttctg ttgtacgata tattatttat aatgttcaat
     1441 ttatattgtt gatgtattat tttaatatag aaatttcaat attatacgca gtgattatat
     1501 cgatgactat ttttttattt cagacgataa atccgttaaa tattgcatta atagattttg
     1561 gttttagagg aaatgttgcc ttgtttttct tgatgccgtt tacggataat agtctaaata
     1621 ttttggcggc aactattttc ttatggtttg taaatctggt aattcctgct atatttggag
     1681 gaatttcagc attgaggttt aaatttgttt cagaagaaca acagtaaaaa taaatatatg
     1741 agtatagatg taatggtaat tttaattggt tctattttct gtggactgat gattttgttt
     1801 acctatttga ccaatagagc tattcaacct aaacaacttt ttctactttt ctttacagaa
     1861 atgtgg
//
LOCUS       DAOQCW010000179         1841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_47491_length_1841_cov_7.338746, whole genome shotgun sequence.
ACCESSION   DAOQCW010000179 DAOQCW010000000
VERSION     DAOQCW010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47491_length_1841_cov_7.338746"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1651
                     /locus_tag="PLS16_11105"
     CDS             <1..1651
                     /locus_tag="PLS16_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNJ61674.1"
                     /translation="EYQKYFEKDKALERRFQKVMVQEPSVEDTISILRGLKERYENHH
                     KVTIKDEAIIAAAELSSRYITNRFLPDKAIDLIDEAAAKLRLEMDSVPEELDELERRI
                     RQLEIEREAIKREDDEVKVQELSKQIAELSEKRNAFKAKWESEKTILDRIQNEKVKIE
                     NFKLEAEQAERNGDYGKVAEIRYGKIKEAENNLNQLEAELQNNKEDASLMKEVVTSEN
                     IAEVVSKWTGIPLTKMLQKEKEKLLNLEEHLHERVVGQDEAIVAVADAIRRSRAGLQD
                     PKKPLGSFLFIGTTGVGKTELAKALAELLFDDEKAIVRIDMSEYMEKHAVSRLIGSPP
                     GYVGYDEGGQLTEAVRNHPYSVVLLDEIEKAHPDVFNVLLQVLEDGRLTDNKGRTVDF
                     KNTIIIMTSNIGSHLIQQNFENVQTDNEIFAATETSKVQILDAIKRVLRPEFYNRIDE
                     IIMFKPLLQNDIKSIVKIQLANLSKQLKAMEIDLDFSNELVNYFTEEGYDVQYGARPL
                     KRLINKEVVNELSKMILADKIQKDKPVIADIFNGNIVFRNE"
BASE COUNT          754 a          238 c          337 g          512 t
ORIGIN      
        1 tgagtatcaa aaatattttg aaaaagataa agccttagag cgtcgtttcc aaaaagtaat
       61 ggtacaagaa cccagtgtag aagataccat ttcaatattg agaggtttaa aagaacgata
      121 tgaaaaccat cataaagtaa ccattaaaga cgaagctatt attgctgctg ctgagttatc
      181 cagtcgttat attaccaatc gttttttacc agataaagcc atcgatttaa ttgatgaagc
      241 tgctgcaaaa ttgagattgg aaatggattc agttccagag gaattagatg aattagaaag
      301 aagaatccga caattagaaa ttgaaagaga agccattaaa cgtgaagatg atgaagtaaa
      361 agttcaagaa ttatctaaac aaattgcaga actttctgaa aaaagaaatg cttttaaagc
      421 taaatgggaa agtgaaaaaa caatattgga tagaatccaa aatgagaaag taaaaattga
      481 gaattttaag ctagaagcag aacaagcgga aagaaatggt gattatggaa aagttgctga
      541 gattcgctat ggcaaaatca aagaagctga aaataatttg aaccagttag aagcagaact
      601 acaaaataac aaagaagatg ctagtttgat gaaagaagtc gtaacgagtg aaaatattgc
      661 tgaggtggtt tcaaaatgga caggtattcc attgactaaa atgcttcaaa aagaaaaaga
      721 aaaattattg aatttagaag agcatttaca cgaaagagtt gttggacaag atgaagcaat
      781 agttgccgta gcagatgcaa ttagaagaag tcgtgctggc ttacaagacc ccaaaaagcc
      841 attaggctct ttcctattta ttggaactac aggcgttgga aaaacagaat tagcaaaagc
      901 tttggctgaa ttattatttg atgatgaaaa agccatcgtt agaattgata tgagtgagta
      961 tatggaaaaa catgctgttt cgagattaat aggttcgcct ccaggatatg ttggatatga
     1021 tgaaggtgga caattgacag aagccgttag aaatcatcca tattctgtgg ttttattaga
     1081 tgaaattgaa aaagcacatc ctgatgtctt caatgtttta ttgcaagtat tagaagacgg
     1141 acgattgacc gacaataaag gcagaactgt tgattttaaa aatacaataa tcatcatgac
     1201 ttccaatatt ggctcacact tgatacaaca aaattttgaa aatgtgcaaa ctgacaatga
     1261 aatttttgct gcaacagaaa cctcaaaagt tcaaatttta gatgcgataa aacgtgtatt
     1321 aagacctgaa ttttacaata gaattgatga aataattatg ttcaaaccac ttttacaaaa
     1381 cgacattaag tctatcgtaa aaatacaatt agctaattta agcaaacaat tgaaggctat
     1441 ggaaattgat ttagatttct ctaatgaatt ggtaaattac tttacagaag aaggatacga
     1501 tgttcaatat ggtgcaagac cattaaaaag attaatcaat aaagaagttg taaatgaatt
     1561 gtctaaaatg atattagccg ataaaattca aaaagacaaa ccagtaattg cagatatttt
     1621 caacggcaat attgtattcc gtaacgaata aaacccatta gggcatgaat aaaaaacatg
     1681 cctttttttt aaacaaaata aggataatta caaatctgaa actcttatta aataaggaac
     1741 aaattcagat caaatacggt tatgtattat acttattgtt ataaataata cgaaatacaa
     1801 tattgcgtat tatattcata aatacaggta atacgcaata a
//
LOCUS       DAOQCW010000180         1603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_62198_length_1603_cov_6.685401, whole genome shotgun sequence.
ACCESSION   DAOQCW010000180 DAOQCW010000000
VERSION     DAOQCW010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62198_length_1603_cov_6.685401"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="PLS16_11110"
     CDS             complement(<1..303)
                     /locus_tag="PLS16_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61675.1"
                     /translation="MRTLFLIITLVFCFYYSSAKPPKKKIKDNHINLDEIVITPTNEF
                     TDYQAARTRDFDLLHTKIEIEPILQNKTANGKATLILKPYFYPKDELILDAKYMQIN"
     gene            complement(420..950)
                     /gene="hpt"
                     /locus_tag="PLS16_11115"
     CDS             complement(420..950)
                     /gene="hpt"
                     /locus_tag="PLS16_11115"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNJ61676.1"
                     /translation="MIQILDKQFKLYIDATTIQNRVMAIADELNKDFVDSRPIILVVL
                     RGAFMFAADILKHLTIPCEIDFVQLSSYDGLSSSGKILLKSEPKTNLENRKVIIIEDI
                     VDSGLTMDFFIDYLKSKNIAHYTLVSLLAKPDNIEKPVHIDYLGFNIPNLFVVGYGLD
                     YNELGRNLDAIYQIVE"
     gene            complement(969..1499)
                     /locus_tag="PLS16_11120"
     CDS             complement(969..1499)
                     /locus_tag="PLS16_11120"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNJ61677.1"
                     /translation="MTKFQIHTITYQTSIGTLVLGEFNGQLCCCDWKNRKLFEQIQKR
                     IQSYTKADFIEKSTDFLSNVTQQLDAYFNRELTTFDISLFFAGTEFQKQVWNELIKIP
                     YGQTTTYLSLSKKLNNESAIRAVASANGANAISIIVPCHRVIGSDGNLVGYAGGLYAK
                     KKLLEIEQFFAQPELF"
BASE COUNT          537 a          233 c          242 g          591 t
ORIGIN      
        1 attgatttgc atatactttg catccaatat taattcatct tttggataga aatagggttt
       61 caagattagt gtagcttttc cgtttgcagt cttattttga aggataggtt ctatttctat
      121 tttagtatgt aataaatcaa aatcccttgt acgagcggct tgatagtctg taaattcatt
      181 ggttggtgta attacaattt cgtctaagtt gatatgatta tcttttatct tttttttggg
      241 cggctttgca gaagaataat aaaagcaaaa tacaagcgtt attattaaga ataatgttct
      301 catttaaaaa tttgtgtaaa tataagttga atagtataga aattataata atataataat
      361 caagatggtt ggatagagta aatgaaagca gtgaagctct tttctattta attagaagac
      421 tattctacaa tttgatagat agcatccaaa tttctaccca actcattata atccaaacca
      481 tagccaacta caaatagatt gggaatgttg aaacctaaat agtcaatgtg aacaggcttc
      541 tctatattgt caggttttgc caataaggaa actaaagtgt aatgtgcaat atttttgctt
      601 ttcaaataat caataaaaaa atccattgtc aaaccagaat caacaatgtc ctcaataatg
      661 attacctttc tgttttctaa gttggttttg ggttcacttt ttaatagtat ttttcccgat
      721 gaacttaatc catcataaga tgataattgc acaaaatcta tttcgcaagg tattgttaga
      781 tgttttagta tatcagcagc aaacataaat gcaccacgta acacgactaa aataattgga
      841 cgactatcaa caaaatcttt attgagttca tcagcaattg ccatcactct attttggata
      901 gttgtagcat caatatataa tttgaattgc ttatctaaaa tttgaatcat aaagcgatta
      961 tttgactgct aaaatagttc aggttgagca aaaaactgct ctatttccaa taattttttc
     1021 ttagcatata gtccaccagc atagcctacc aaattgccat cactcccaat cactcgatga
     1081 caaggaacta tgatggatat tgcatttgca ccatttgcac tcgcaacggc acgaatagca
     1141 ctttcattat tgagtttttt tgataaactt aaataggttg ttgtttgtcc gtaaggaatt
     1201 ttgattaatt cattccaaac ttgcttttga aattcagtgc ctgcaaaaaa taatgaaata
     1261 tcaaaagttg taagttctct gttaaaatat gcatcgagtt gttgggtaac attacttaaa
     1321 aaatcagtac ttttttcaat aaaatcggct ttagtataag attgaattct tttttgtatt
     1381 tgttcaaata gctttcgatt tttccaatca caacaacaca attgaccatt aaattctcct
     1441 aaaactaatg tgccaattga agtttggtat gtaattgtat ggatttgaaa tttagtcaaa
     1501 gtaatttttt tgaatgatta ttaatacttt ctacgcacat taattgtctg aactttgatt
     1561 tttgtgatta aatgatggac aggatttatt ttagttgtta gtt
//
LOCUS       DAOQCW010000181         1500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0772
            NODE_70881_length_1500_cov_5.438062, whole genome shotgun sequence.
ACCESSION   DAOQCW010000181 DAOQCW010000000
VERSION     DAOQCW010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810404
            Sequence Read Archive: SRR3501885
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:02:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,225
            CDSs (total)                      :: 2,197
            Genes (coding)                    :: 2,193
            CDSs (with protein)               :: 2,193
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70881_length_1500_cov_5.438062"
                     /isolate="AMR_MDS_0772"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..>1500)
                     /locus_tag="PLS16_11125"
     CDS             complement(77..>1500)
                     /locus_tag="PLS16_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNJ61678.1"
                     /translation="ILNTTDTFYVSSVIGMVAPGQLRRSFLAYLERERAVPWRSFVLY
                     NSWYELNIDRNDNPNPLKRMTEQQCLPILDAWENKLYKPHGISIDAFVWDDGWDDFNS
                     LWDFHIGFPQGFSKLSAKAQEQKSGIGTWLGPVGGYGASKAQRLAFWNKTHSPPIKNF
                     QLSNKEYFDAFIGRCSQMIKDYDMRYFKFDGISDYFSATGPKNEEDAEGILKILSALR
                     KKRENIFFNCTVGTWASPFWLQYGDAIWRQEGDFGMIGKQGNSREKWITYRDRLVYQN
                     FVKNSPLCPINSLMTHGLIVTQHGPPTEMSYDNSPATFKEIVKEMRCAFACGTSMVEL
                     YLDNDLMSKIGGDDKLWSELASCITWHRNNADVLADTHWVGGNPWDGSKANIYGWAAW
                     NENKATLTLRNPSGFTQTFKITLRKALDIPDFIKGEIFLSSAFPDQNIYKNISEQKID
                     IDTIITFEMPAFDVIVFNGKKVN"
BASE COUNT          483 a          297 c          220 g          500 t
ORIGIN      
        1 ttaataatga cgaaattaaa tttcctacat caaaaggttt gagttataca attagcgaat
       61 taaaataaag aaacgcttag tttactttct tcccattgaa tacaataaca tcaaatgcag
      121 gcatttcaaa tgttataatc gtatcaatgt ctattttctg ttctgaaatg tttttataaa
      181 tattttggtc tggaaaagct gatgacaaaa agatttctcc tttaataaaa tcaggaatat
      241 ctaaggcttt acgtaaggtt attttaaatg tttgagtgaa cccacttgga ttacgtaagg
      301 ttaaagttgc tttattttca ttccatgctg cccaaccata aatatttgct ttagaaccat
      361 cccaaggatt tcctcccacc caatgtgtgt ctgccaatac atctgcatta ttacgatgcc
      421 aagtaataca acttgctagc tcgctccata gtttatcatc tcctccaatt ttactcatta
      481 agtcgttatc taaatataat tctaccatac ttgtgccaca agcaaaagca caacgcattt
      541 cttttactat ttctttaaac gtagcaggtg aattatcata agacatttct gttggaggtc
      601 cgtgttgtgt aacgattaac ccatgtgtca taagtgaatt aatagggcac agaggagagt
      661 tttttacaaa attttgataa actaatctat ctcgataggt tatccatttc tctctgctat
      721 taccttgctt accaatcata ccgaaatcgc cttcttgtcg ccatattgca tcgccatatt
      781 gcaaccaaaa aggagatgcc caagtaccaa ctgtacaatt aaaaaagata ttttctcttt
      841 ttttgcgtaa agcactaagt attttcaaaa tcccttcggc atcttcttca ttctttggac
      901 ctgtggcact aaagtaatca ctaattccgt caaatttaaa gtaacgcatg tcataatcct
      961 ttatcatctg agaacagcga cctataaacg catcaaaata ttctttattg gataattgaa
     1021 aatttttgat aggtggcgaa tgtgttttat tccaaaaagc taatctttga gctttagatg
     1081 ctccataccc tcctactgga cccaaccatg taccaatgcc acttttctgt tcttgtgctt
     1141 ttgcacttaa cttagaaaag ccttgtggaa acccaatatg aaaatcccat aaactattaa
     1201 aatcatccca accgtcatcc catacaaaag catctatact tattccatga ggtttatata
     1261 atttattttc ccatgcatct agtattggta aacattgttg ttcagtcatt cgctttaaag
     1321 ggttaggatt gtcattccta tcgatattta attcatacca agaattatat aaaacaaagg
     1381 aacgccaagg aacagcacgt tctcgctcaa gataagctag aaaagagcga cgaagttgtc
     1441 caggtgcaac cattcctata acactagaaa cataaaaggt gtctgttgta tttaagatag
//
